LOCUS       DAMZLE010000001        90242 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMZLE010000001 DAMZLE010000000
VERSION     DAMZLE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 90242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.001"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            144..2249
                     /gene="clpL"
                     /locus_tag="POB92_000001"
     CDS             144..2249
                     /gene="clpL"
                     /locus_tag="POB92_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDI6577531.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(2318..2488)
                     /locus_tag="POB92_000002"
     CDS             complement(2318..2488)
                     /locus_tag="POB92_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDI6577532.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(2501..4495)
                     /gene="feoB"
                     /locus_tag="POB92_000003"
     CDS             complement(2501..4495)
                     /gene="feoB"
                     /locus_tag="POB92_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDI6577533.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVDKQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVVYPLVTAYVLTFIFYQIGHLFV"
     gene            complement(4507..4734)
                     /locus_tag="POB92_000004"
     CDS             complement(4507..4734)
                     /locus_tag="POB92_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDI6577534.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4950..7439)
                     /gene="farE"
                     /locus_tag="POB92_000005"
     CDS             complement(4950..7439)
                     /gene="farE"
                     /locus_tag="POB92_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDI6577535.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKKGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKIY
                     PQGATNVSKHQDVHGQDDVRSIVLDDKTMALYQEVKQQSASSFFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7584..8132
                     /gene="farR"
                     /locus_tag="POB92_000006"
     CDS             7584..8132
                     /gene="farR"
                     /locus_tag="POB92_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDI6577536.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTISDTINNKEDLRDIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFMHWIKDE
                     KIDWPGEEIDKIFHKVINIKIK"
     gene            complement(8208..8375)
                     /locus_tag="POB92_000007"
     CDS             complement(8208..8375)
                     /locus_tag="POB92_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577537.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISYF
                     NSDKHDKNKKR"
     gene            complement(8487..10031)
                     /gene="pruA"
                     /locus_tag="POB92_000008"
     CDS             complement(8487..10031)
                     /gene="pruA"
                     /locus_tag="POB92_000008"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDI6577538.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     GILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10221..10820)
                     /locus_tag="POB92_000009"
     CDS             complement(10221..10820)
                     /locus_tag="POB92_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDI6577539.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            11059..11250
                     /gene="cwrA"
                     /locus_tag="POB92_000010"
     CDS             11059..11250
                     /gene="cwrA"
                     /locus_tag="POB92_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDI6577540.1"
                     /translation="MRILITGTVAILIILGLVKTIQDHEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11490..13898
                     /locus_tag="POB92_000011"
     CDS             11490..13898
                     /locus_tag="POB92_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDI6577541.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTYYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            14247..14453
                     /gene="copZ"
                     /locus_tag="POB92_000012"
     CDS             14247..14453
                     /gene="copZ"
                     /locus_tag="POB92_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDI6577542.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(14543..15541)
                     /locus_tag="POB92_000013"
     CDS             complement(14543..15541)
                     /locus_tag="POB92_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6577543.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELDIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(15564..16718)
                     /locus_tag="POB92_000014"
     CDS             complement(15564..16718)
                     /locus_tag="POB92_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6577544.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQQALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(17145..18653)
                     /locus_tag="POB92_000015"
     CDS             complement(17145..18653)
                     /locus_tag="POB92_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6577545.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            complement(18665..19528)
                     /locus_tag="POB92_000016"
     CDS             complement(18665..19528)
                     /locus_tag="POB92_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDI6577546.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(19565..20692)
                     /gene="crtQ"
                     /locus_tag="POB92_000017"
     CDS             complement(19565..20692)
                     /gene="crtQ"
                     /locus_tag="POB92_000017"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDI6577547.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(20698..22191)
                     /locus_tag="POB92_000018"
     CDS             complement(20698..22191)
                     /locus_tag="POB92_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6577548.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGIPMVTLSGQQVADKINAREAKNRK"
     gene            complement(22184..22672)
                     /gene="crtO"
                     /locus_tag="POB92_000019"
     CDS             complement(22184..22672)
                     /gene="crtO"
                     /locus_tag="POB92_000019"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDI6577549.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(22847..23614)
                     /locus_tag="POB92_000020"
     CDS             complement(22847..23614)
                     /locus_tag="POB92_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6577550.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(23975..25786)
                     /locus_tag="POB92_000021"
     CDS             complement(23975..25786)
                     /locus_tag="POB92_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6577551.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(26299..26787)
                     /locus_tag="POB92_000022"
     CDS             complement(26299..26787)
                     /locus_tag="POB92_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6577552.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            complement(27063..27764)
                     /gene="isaA"
                     /locus_tag="POB92_000023"
     CDS             complement(27063..27764)
                     /gene="isaA"
                     /locus_tag="POB92_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDI6577553.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(28372..29427)
                     /locus_tag="POB92_000024"
     CDS             complement(28372..29427)
                     /locus_tag="POB92_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDI6577554.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGISGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(29545..29682)
                     /locus_tag="POB92_000025"
     CDS             complement(29545..29682)
                     /locus_tag="POB92_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577555.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYRCY
                     C"
     gene            29837..30406
                     /locus_tag="POB92_000026"
     CDS             29837..30406
                     /locus_tag="POB92_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6577556.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            30604..31104
                     /locus_tag="POB92_000027"
     CDS             30604..31104
                     /locus_tag="POB92_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577557.1"
                     /translation="MTSIFVFAYLNNTSDNMLFRQQMICACLLANVQHSSIVCLNNTC
                     CIVDIRCLNTKIGGIDVSETDEPQGFERTHNILNINQNRQSLMTFIISKLKSTLKQRI
                     INARGKKRIDYRLSYNFYITYYDTVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            31324..31554
                     /locus_tag="POB92_000028"
     CDS             31324..31554
                     /locus_tag="POB92_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDI6577558.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(31717..32094)
                     /locus_tag="POB92_000029"
     CDS             complement(31717..32094)
                     /locus_tag="POB92_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6577559.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(32111..32932)
                     /locus_tag="POB92_000030"
     CDS             complement(32111..32932)
                     /locus_tag="POB92_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDI6577560.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(32951..33250)
                     /locus_tag="POB92_000031"
     CDS             complement(32951..33250)
                     /locus_tag="POB92_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDI6577561.1"
                     /translation="MSLNKEQRRITCEELQAHFEESTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            33382..33939
                     /locus_tag="POB92_000032"
     CDS             33382..33939
                     /locus_tag="POB92_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6577562.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            33932..34636
                     /locus_tag="POB92_000033"
     CDS             33932..34636
                     /locus_tag="POB92_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6577563.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            34734..35744
                     /locus_tag="POB92_000034"
     CDS             34734..35744
                     /locus_tag="POB92_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6577564.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSA
                     NN"
     gene            35764..36582
                     /locus_tag="POB92_000035"
     CDS             35764..36582
                     /locus_tag="POB92_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6577565.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     IVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGMKTFG
                     ETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKYSDKI
                     TLLNGTDSRGSFPQDVNFYINKETSIPIVDIPGGHLGYIQKPEGFADVLLNMWG"
     gene            complement(36729..37619)
                     /locus_tag="POB92_000036"
     CDS             complement(36729..37619)
                     /locus_tag="POB92_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6577566.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPAKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(37716..39056)
                     /locus_tag="POB92_000037"
     CDS             complement(37716..39056)
                     /locus_tag="POB92_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDI6577567.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTVCSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(39050..40156)
                     /locus_tag="POB92_000038"
     CDS             complement(39050..40156)
                     /locus_tag="POB92_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDI6577568.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(40451..40747)
                     /locus_tag="POB92_000039"
     CDS             complement(40451..40747)
                     /locus_tag="POB92_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDI6577569.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(40735..41103)
                     /locus_tag="POB92_000040"
     CDS             complement(40735..41103)
                     /locus_tag="POB92_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577570.1"
                     /translation="MSNKLDEINKIITAKHKQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(41118..41396)
                     /locus_tag="POB92_000041"
     CDS             complement(41118..41396)
                     /locus_tag="POB92_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDI6577571.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            complement(41735..42601)
                     /locus_tag="POB92_000042"
     CDS             complement(41735..42601)
                     /locus_tag="POB92_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6577572.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMNKILQDTTS"
     gene            42819..43883
                     /locus_tag="POB92_000043"
     CDS             42819..43883
                     /locus_tag="POB92_000043"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDI6577573.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            44038..44313
                     /locus_tag="POB92_000044"
     CDS             44038..44313
                     /locus_tag="POB92_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577574.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(44499..45221)
                     /locus_tag="POB92_000045"
     CDS             complement(44499..45221)
                     /locus_tag="POB92_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDI6577575.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            45575..46024
                     /locus_tag="POB92_000046"
     CDS             45575..46024
                     /locus_tag="POB92_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDI6577576.1"
                     /translation="MTALFPYIAFENSKEAIAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(46105..46692)
                     /locus_tag="POB92_000047"
     CDS             complement(46105..46692)
                     /locus_tag="POB92_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6577577.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(46670..46778)
                     /locus_tag="POB92_000048"
                     /pseudo
     CDS             complement(46670..46778)
                     /locus_tag="POB92_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            46835..48517
                     /locus_tag="POB92_000049"
     CDS             46835..48517
                     /locus_tag="POB92_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDI6577578.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQHQVQLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAVSEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAYVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(48615..49211)
                     /locus_tag="POB92_000050"
     CDS             complement(48615..49211)
                     /locus_tag="POB92_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6577579.1"
                     /translation="MLIFTDNANAENNKPEGVNENSNLIPVRQPDANYPGPVSDIARV
                     KKLDNLIDGTKDPDVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLLKHPDNLIDGTKDP
                     NVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDSKSKSVHKQQLKEL
                     PKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ"
     gene            complement(49332..49715)
                     /locus_tag="POB92_000051"
     CDS             complement(49332..49715)
                     /locus_tag="POB92_000051"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI6577580.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIQNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(49717..50568)
                     /locus_tag="POB92_000052"
     CDS             complement(49717..50568)
                     /locus_tag="POB92_000052"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI6577581.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(50561..51379)
                     /gene="panB"
                     /locus_tag="POB92_000053"
     CDS             complement(50561..51379)
                     /gene="panB"
                     /locus_tag="POB92_000053"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI6577582.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            51452..52312
                     /locus_tag="POB92_000054"
     CDS             51452..52312
                     /locus_tag="POB92_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6577583.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(52398..53102)
                     /gene="budA"
                     /locus_tag="POB92_000055"
     CDS             complement(52398..53102)
                     /gene="budA"
                     /locus_tag="POB92_000055"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI6577584.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFSLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQNIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(53380..54339)
                     /locus_tag="POB92_000056"
     CDS             complement(53380..54339)
                     /locus_tag="POB92_000056"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI6577585.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            55026..56474
                     /locus_tag="POB92_000057"
     CDS             55026..56474
                     /locus_tag="POB92_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6577586.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(56554..57891)
                     /locus_tag="POB92_000058"
     CDS             complement(56554..57891)
                     /locus_tag="POB92_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDI6577587.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEITPGDF
                     EKRVTFDLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDGSAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            58149..58565
                     /locus_tag="POB92_000059"
     CDS             58149..58565
                     /locus_tag="POB92_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577588.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPFILEIICHLLIGSLIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            58688..59578
                     /locus_tag="POB92_000060"
     CDS             58688..59578
                     /locus_tag="POB92_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDI6577589.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDNLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(59772..61268)
                     /gene="mqo"
                     /locus_tag="POB92_000061"
     CDS             complement(59772..61268)
                     /gene="mqo"
                     /locus_tag="POB92_000061"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6577590.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            61564..61893
                     /locus_tag="POB92_000062"
     CDS             61564..61893
                     /locus_tag="POB92_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577591.1"
                     /translation="MDYSILNLYSILIQYKCKINHKKEIQYLHPRKTSQLPLILKTIL
                     NLSLIGIVIIVFTHAEFQLNAGDSNVFLLTWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(61953..63551)
                     /locus_tag="POB92_000063"
     CDS             complement(61953..63551)
                     /locus_tag="POB92_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6577592.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(63745..64188)
                     /locus_tag="POB92_000064"
     CDS             complement(63745..64188)
                     /locus_tag="POB92_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI6577593.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(64468..64680)
                     /locus_tag="POB92_000065"
     CDS             complement(64468..64680)
                     /locus_tag="POB92_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6577594.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(64958..66667)
                     /gene="betA"
                     /locus_tag="POB92_000066"
     CDS             complement(64958..66667)
                     /gene="betA"
                     /locus_tag="POB92_000066"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDI6577595.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDAKEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(66928..68418)
                     /gene="betB"
                     /locus_tag="POB92_000067"
     CDS             complement(66928..68418)
                     /gene="betB"
                     /locus_tag="POB92_000067"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDI6577596.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            68487..68582
                     /locus_tag="POB92_000068"
     CDS             68487..68582
                     /locus_tag="POB92_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577597.1"
                     /translation="MKNVSIYKTTQELSFVSLLDFYLTKHLTLML"
     gene            68671..69234
                     /locus_tag="POB92_000069"
     CDS             68671..69234
                     /locus_tag="POB92_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDI6577598.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(69422..70108)
                     /locus_tag="POB92_000070"
     CDS             complement(69422..70108)
                     /locus_tag="POB92_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6577599.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(70367..71989)
                     /locus_tag="POB92_000071"
     CDS             complement(70367..71989)
                     /locus_tag="POB92_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6577600.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            72121..72282
                     /locus_tag="POB92_000072"
     CDS             72121..72282
                     /locus_tag="POB92_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577601.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKTIVLSYDLTNLTNFTRTAFKNIY
                     TLLTNIYLL"
     gene            complement(72298..72408)
                     /locus_tag="POB92_000073"
     CDS             complement(72298..72408)
                     /locus_tag="POB92_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577602.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(72508..73044)
                     /gene="nrdG"
                     /locus_tag="POB92_000074"
     CDS             complement(72508..73044)
                     /gene="nrdG"
                     /locus_tag="POB92_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDI6577603.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNKKTIWVWTGFLYEYLANDCAERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(73041..74891)
                     /gene="nrdD"
                     /locus_tag="POB92_000075"
     CDS             complement(73041..74891)
                     /gene="nrdD"
                     /locus_tag="POB92_000075"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI6577604.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNYLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(75137..76507)
                     /locus_tag="POB92_000076"
     CDS             complement(75137..76507)
                     /locus_tag="POB92_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDI6577605.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLIIMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILIMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(76983..77588)
                     /locus_tag="POB92_000077"
     CDS             complement(76983..77588)
                     /locus_tag="POB92_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDI6577606.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGLVASRRAQTLSQYVEHIMVISPEI
                     TEKLQNMVDKGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDMNHSEKQQLLSQIVTKEFINDTKQAQFLAWLDKK"
     gene            complement(77651..79531)
                     /locus_tag="POB92_000078"
     CDS             complement(77651..79531)
                     /locus_tag="POB92_000078"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDI6577607.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQQQRYQRD
                     VY"
     gene            79725..79817
                     /locus_tag="POB92_000079"
     CDS             79725..79817
                     /locus_tag="POB92_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577608.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(79925..80422)
                     /locus_tag="POB92_000080"
     CDS             complement(79925..80422)
                     /locus_tag="POB92_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6577609.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(80432..80692)
                     /locus_tag="POB92_000081"
     CDS             complement(80432..80692)
                     /locus_tag="POB92_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577610.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(81074..81187)
                     /locus_tag="POB92_000082"
                     /pseudo
     CDS             complement(81074..81187)
                     /locus_tag="POB92_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(81846..83846)
                     /locus_tag="POB92_000083"
     CDS             complement(81846..83846)
                     /locus_tag="POB92_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577611.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFIIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDFVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(83843..84598)
                     /locus_tag="POB92_000084"
     CDS             complement(83843..84598)
                     /locus_tag="POB92_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577612.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(84706..85593)
                     /gene="nsaS"
                     /locus_tag="POB92_000085"
     CDS             complement(84706..85593)
                     /gene="nsaS"
                     /locus_tag="POB92_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDI6577613.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLLIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(85604..86269)
                     /gene="nsaR"
                     /locus_tag="POB92_000086"
     CDS             complement(85604..86269)
                     /gene="nsaR"
                     /locus_tag="POB92_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDI6577614.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(86295..86495)
                     /locus_tag="POB92_000087"
     CDS             complement(86295..86495)
                     /locus_tag="POB92_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577615.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
     gene            86693..88117
                     /locus_tag="POB92_000088"
     CDS             86693..88117
                     /locus_tag="POB92_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDI6577616.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKNDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAKVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            88187..88297
                     /locus_tag="POB92_000089"
     CDS             88187..88297
                     /locus_tag="POB92_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577617.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(88400..88855)
                     /locus_tag="POB92_000090"
     CDS             complement(88400..88855)
                     /locus_tag="POB92_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6577618.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(89100..89861)
                     /locus_tag="POB92_000091"
     CDS             complement(89100..89861)
                     /locus_tag="POB92_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6577619.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDITAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(89977..>90242)
                     /locus_tag="POB92_000092"
     CDS             complement(89977..>90242)
                     /locus_tag="POB92_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6577620.1"
                     /translation="DSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
BASE COUNT        28977 a        16756 c        13678 g        30831 t
ORIGIN      
        1 aatattcata aagttttaaa aatattctgt ttaatcatct attattagtg gaaaaatata
       61 agagcaattg tttatagttt gcataacgct tcaaaagtat tttctttttt gtttagttca
      121 aaaaaattta gaggtgatgt tatatgaata acggtttttt caatagcgac tttgattcaa
      181 ttttccgaag aatgatgcaa gatatgcaag gttcaaatca agtcggtaac aaaaagtact
      241 atattaatgg taaagaagtt tcacctgaag aactagcgca actcacacaa caaggtggca
      301 atcactctgc tgaacaaagt gcgcaagctt ttcaacaagc agcacaaaga caacaagggc
      361 aacaaggtgg caacggcaat tatttagaac aaattggtcg taaccttacg caagaagcac
      421 gtgacggttt attagatcca gtcattggtc gtgataaaga aattcaagaa actgctgaag
      481 tcttaagtag acgaactaaa aacaatccta tattagttgg agaagctggt gttggtaaaa
      541 ctgcgattgt tgaaggttta gcacaggcaa tcgttgaagg aaatgtacca gcagcaatca
      601 aagacaaaga aattatttct gtagatattt catcattaga agctggaacg caatatcgtg
      661 gtgcttttga agaaaatatt caaaaattaa tcgaaggtgt taaatcttca caaaatgccg
      721 tattattctt tgatgaaatc catcaaatta tcggttcagg tgccacagga agtgattcag
      781 gtagcaaagg gttatctgat attttgaaac ctgcattaag tcgtggtgaa atttcaatta
      841 ttggtgcaac aacacaagat gaatatcgaa acaatattct taaagatgct gcattaacgc
      901 gcagatttaa tgaagtactt gttaatgaac caagcgctaa agatactgtt gaaattttaa
      961 aaggtattcg cgaaaaattc gaagaacacc atcaagtaaa attaccagat gacgtattaa
     1021 aagcatgtgt tgacttatca attcaatata ttccacaacg attattacca gataaagcaa
     1081 tcgatgtgtt agatattaca gcagcacatt tatctgcgca aagtccagct gtcgataaag
     1141 ttgaaactga aaaacgaatt tctgaattag aaaatgataa acgtaaagcg gtaagtgctg
     1201 aagaatataa aaaagctgac gacattcaaa atgaaatcaa atcattacaa gataaattag
     1261 aaaatagtaa tggtgaacat actgctgttg ctacagttca tgatatttca gatactattc
     1321 aacgattaac tggtattcca gtttctcaaa tggatgataa cgatattgaa cgtttaaaaa
     1381 atatttctaa tcgtttaaga agtaaaatca taggtcaaga tcaagctgta gaaatggttt
     1441 cacgtgcaat tcgccgtaat cgtgctgggt ttgatgacgg caaccgccca attggcagtt
     1501 tcctatttgt tggccctact ggtgttggta aaacagagct tgctaaacaa ttagcaattg
     1561 atctatttgg taataaagat gcactgattc gacttgatat gagtgaatat agtgacacaa
     1621 cagctgtttc aaaaatgatt ggtacaactg ctggttatgt cggttatgat gacaattcaa
     1681 acacgttaac tgaaaaagta cgccgtaatc catactcagt cattctattt gatgaaatcg
     1741 aaaaagcaaa tccacaaatt ttaacattgt tattacaagt aatggatgat ggtaatttga
     1801 ctgatggtca aggtaatgtc atcaacttta aaaatacaat tattatttgt acatcaaatg
     1861 ctggctttgg caatggcaat gacgctgaag aaaaagatat tatgcacgaa atgaaaaaat
     1921 tcttccgccc tgaattcctt aaccgcttca acggcatcgt tgaattctta catttagata
     1981 aagatgcatt gcaagatatc gtcaacttat tattagacga tgtacaagtt acattagata
     2041 aaaaaggtat tacgatggac gtttctcaag atgcgaaaga ttggttaatt gaagaaggct
     2101 atgatgaaga attaggtgca cgtccattaa gacgtattgt tgaacagcaa gtacgtgaca
     2161 aaattacaga ttactattta gatcatacag acgttaaaca tgtggatata gatgttgagg
     2221 ataacgaatt agtcgtaaaa ggtaaataac gacactttaa catatcgcgc atcaaaaatg
     2281 agcatcaggt cgcccttgcc tgtgctcatt tttttaatta tttccctgga aaatgattcg
     2341 ctgtgtgctg ttctgttcca caacaatcac gattaatgtc acatgtacca cattttcctt
     2401 gtttcgaacg tttgaaaaat ttcactagtg tatacaaggc atatccgaaa attgctaaaa
     2461 aaattaaaat gttaataatg actgacactt taaccactcc ttaaacaaat aaatgtccga
     2521 tttgataaaa aatgaatgtt aagacatatg cagtgactag aggatagaca actgcaagtg
     2581 ccgtccattt ccatgaataa gtctctttac ggattgctgc cactgtagaa acacaaggaa
     2641 tatacaatag tataaatatc ataaatgcat acgcagatag cggtgtgaat tgattttgaa
     2701 tcacattaac aaggcctgca tcacctgatg aatagataat cgccatcgaa cttacgataa
     2761 cttcttttgc taaaaatcct ggcactaacg tagcacctgc ttgccatgtt ccaaatccga
     2821 gcggttgcac taacatacca aagaaactac caaccatatg taaaaaactt tgattgatat
     2881 tcacattgat accatgtggt cctacataac ttaatagcca aatgactact gagccgccaa
     2941 aaataaatgt acctgcttta cgaacaaagc ccttagcctt ttcccaagta ctacgccaca
     3001 acgttttaat ggaaggcaca cggtatgttg gcaattccac aataaagatt gcattatcat
     3061 tttttaaaat cgtcttagta agtactgtac tgactaaaaa tgccataata atacctaaaa
     3121 catacaagct taatactact aaagattgat tctctttgaa aaagatacct acgaacaacg
     3181 catacactgg cagtctagca gagcatgaca tgaatggtgc aattaatatc gttgttaaac
     3241 gctctttttc attttcaata ctgcgcgcag ccataatact cggtacgtta caaccaaatc
     3301 cgataatcat cggtataaaa gacttcccgc ttaaaccgaa cgattccata atacgatcca
     3361 ttaaaactgc aatacgcgcc atataacctg aatcttctaa taaagatata aagaaaaata
     3421 gcacaacaat ttgtggtaca aagactaata ctgatcctac accagcaata atgccatctg
     3481 taattaaatc ttgtaaaaat ggtataacgc caagataatt cataatcgtc ttcacactat
     3541 ctgtaaatgt accacctata aatgcatcga gttgatccga caaaggtgtg ccaatccatg
     3601 taaatgtagt ttgaaagatc aaccacataa tcgctagaaa gataggcatc cctatatatt
     3661 tatgtgttaa tatcttgtct attctagagc tgaaatattg cttatcttca tctggatacg
     3721 ttaccacgtc ttgcaataac gtctcaatat aatgattgcg tatacgctcc atctctcgac
     3781 gaacagatac agcctctact tgtttatcaa cttgatcacg taaactcgac aatttattta
     3841 caacctctga attaagttcg tttgcaattt gcatgttatt taataagaat tgaatcgcaa
     3901 taaatctagc ttgatattta tcatgagatg tctctgtcat tattatttga cacatatttt
     3961 taattgtctc ttcaatcttc tcaccataat tgattttaaa atgcggttga tacccttccc
     4021 ctagatgctt tatttcgcca agtaaatgtt tagttccttt gcctgtacgt gccacaactg
     4081 gaaaaatagg tgtttttaac tttttcatca atttatgata atcgattttt atcccgcgct
     4141 ttgtagctac atcaatcata tttaatccga tgtatattgg ttgattaagt tctaacaatt
     4201 gtactgttaa ttgcatattc ctttttagtt gactcgcgtc aacaatgtta atgattcctg
     4261 aaaatgaatc gtttaataaa tagtctgtca ctacagtttc atctttagaa atcggcgata
     4321 aatcatatgt acctggtaaa tcaattaatt gtcctacatt ttcttttagc ttccctactt
     4381 ttttctctac cgttacgcca ctccagttgc ctatatattc atacgaacca gttaaagcgt
     4441 taaacaaaga tgttttacca acattagggt ttcctaaaat acaataattt tccattcgtc
     4501 cggctcctat tcttctaatg caatagaaca agcatcgcaa tgcctaatac ttaactgttg
     4561 tccgtttact tcaataatac atggcccttt aaataaacat ttttgtttaa tcgttatgat
     4621 agcgtcatct gttaacccaa aggcacttag acgatacaac atattctcat tagcaatatc
     4681 cattcgcttt attttataag ccttattcat ttcaccattt ttaatgttta acatactatt
     4741 tcgctctcct attagaaata ataatcatta tcacttaaaa atcataaccc ttaaaattgt
     4801 agctcgcaat actttattta aataattttc atttttcatg taaaatttgt gacattgcaa
     4861 aaatgttttg tataatcact ttcatgcaaa aaacctccgc ttcaaatgtc attgatagac
     4921 atttaaagcg gaggcatgta catgttattt tatgatttat ttcgtagatt tttttctaac
     4981 aattgattga gcttttcaat atttttattc aactgttcta tattcgttac ttgttgtttt
     5041 gaatttagtt gatgcttgtt ttgataatct attaaagcat catataagaa aaatgaactt
     5101 gctgattgct gtttcacttc ttggtacaat gccatagttt tatcatctag tactattgaa
     5161 cgtacatcat cttgcccatg tacatcttga tgtttcgata catttgttgc accttgtgga
     5221 tatattttaa aggttgtgtc gtatgtttcc ttactatctt tagcttcaga ttcctgtgat
     5281 tcaaccgttt tatatttttc aagtgcatgt ccttcaatat caactcgtgg aataatgcga
     5341 ttcaaccatg ctggtaaata ccacgaacct tttccaaaca atttcgttaa tgcaggaatt
     5401 aacatcattc tgactacgaa ggcatcaaag agtacaccaa acgctaatgc catacccatt
     5461 gatttaatca tgacatcttc ttggaataca aacgcaaaga agacactaaa catgattaat
     5521 gcagctgcta caataacagg accgctttct ttcaacccta ctttgataga ataatcatta
     5581 tcccctgttt tactatattc ttcatgaatt cgcgacataa ggaagacttc ataatccatc
     5641 gctaatccaa ataagatacc tatagtaata accggtaaaa atgctagcat tggtcctgtc
     5701 gtttcaatac caaacagacc tttcataaaa ccatcttgca ttactaatgt tgtaaatcct
     5761 agcgttgcca ttaatgacaa gacaaatcct aaaactgctt ttaacggtat tagaattgaa
     5821 cggaagacaa tcattaataa gaaaaatgcc aatacaacaa ttactgttgc aaataaaggt
     5881 atcgcctcat ttaacttttt agacatatca atattaatga cactttgtcc cgaaatctcc
     5941 gttttgaacc catatttatc ttgtgcatct ttatgataat ctcgtaaatc atgcactaaa
     6001 tcatttgtac tctctgcatt aggccctttc ttaggtatca cgaccatcaa agcgtaatca
     6061 ttatctttac tcatttgtgg cggcgtaacg atatctacat ttttcttatc tttaatatct
     6121 ttatatacag actgtaaatc ttgttgtaat ccttgtggat catccttttt atctttcaca
     6181 tttatcaaca tcggaatttg gccattaaat ccttcaccaa atttatccga gattatatcg
     6241 taagcttttt tctgtgtaga atctgctggt ttaacaccgt catctggaat accaagtcgc
     6301 atatgactaa ctggtattgc agctgctact aatatgatta aacctagtaa tactgccgca
     6361 agtgcatttc ctgtaataaa tttagaccaa ggcgtatcaa tatctttttt aaaattagac
     6421 tgcaatttgt tcactttaat gcgtttatgg aaaatactaa ttaacgcagg taataaagtc
     6481 agtgcactga atacagcaaa tataacacta attgccgaag caaaccccat cactgctaag
     6541 aaatctatgc caactagcga taaaccgcat acggcaatca caactgtcac accagcgaag
     6601 atgactgcgc tacctgctgt tcctattgca agaccaatgc ctttaatgta atctgtttca
     6661 gttttcataa cttgtcgata tctgaataaa ataaataatg cataatcgat accaactgct
     6721 agtccaatca ttacggctaa tgtcagtgtg acatttggta tatcgaatgc ataagttaac
     6781 aaactgataa tgccaacccc agatgctaga ccaatcaatg cacttataat tggtaatcct
     6841 gcagcaatga ctgaaccaaa tgtaatcaat aacactacaa atgcaacaat gataccaact
     6901 agttcagaat taccgcctac ttctgtagat gtcattcctg taccagttaa ctctgtttgt
     6961 acattatgat tgtcacgtaa gtctttcaaa tgacttttaa cattatctct agagccatct
     7021 tttaaagatg tttgactaac gtcatatgtg atatctgcaa atgcagttgt tttatcttta
     7081 ctaatttgct tattttcata aggatctgat attttatcaa tgtgcttgtc atctttttta
     7141 atatcatcta acgttttctt aatatcttta gtaatgttcg gttgcacaat accatcatct
     7201 ttagtcgtct taaagacaac acgtatttgt gccttttcac tatcttgatt aaaatgtttt
     7261 tcaatctttt tattcgtatc caacgactct aatcctgtca ttttaatatc attgtcaaat
     7321 ttcggtgcat ttgtagcaag tggtatcaat attgcagcta caatcactat ccatgcaatg
     7381 accgcggacc atttatgttt tgcgatgaat gtccccatct tatataaaaa ttttgccaaa
     7441 gtatattgcc tccttttaaa atcaacgtta tagtttaaat atacagtgta gattattgtt
     7501 agattatagt atctatcccc gacctcttaa agaatcaatt ggaaaatttt gtatattaaa
     7561 ctacacacaa aggagaaatg tagatgaaag agactgattt acgagttata aagacaaaaa
     7621 aagcattgtc gagtagcttg ctacaattgt tagaacagca attattccaa acgattactg
     7681 tcaatcaaat ttgcgacaac gcactcgtac accgcacaac attttataaa catttttatg
     7741 ataaatacga tcttctagag tatttgttca atcaattgac taaagactat tttgccagag
     7801 acattagcga tcgccttaac cacccatttc aaacgattag cgatacaatt aacaataaag
     7861 aagatttgcg agatattgca gaatttcaag aagaagacgc tgaatttaat aaagtattaa
     7921 aaaatgtctg cattaaaatt atgcataacg atatcaaaaa taatagagac cgtatcgata
     7981 ttgacagcga catcccagat aatctcatat tttatattta tgactcgttg attgaaggtt
     8041 ttatgcattg gataaaagat gaaaaaattg actggcctgg ggaagaaatt gataaaattt
     8101 tccataaggt aataaatatt aagattaaat agtagatgag aaactcatga gcgttaccaa
     8161 cattcataat aaaaacgata gtgtacacgt taatgaattc gtgtacacta tcgtttttta
     8221 tttttatcat gcttatcgct attaaaataa ctgatacaca acacataaac tatgaagaaa
     8281 aaaataaatc cgctatctaa atgactttga ctcagttgtt taaatgacca aattgctaat
     8341 acaattccca ttattattga aataacgtat ctcacattct tatacctata atccttttct
     8401 aaaaatatgg ttgctattac ttaattttta aagttataaa taaaaagagc caaccgcaat
     8461 ggatggccct tgttcattat gaagcattag aacatttctg aaacaacctt ttgttctaag
     8521 aagtgtaata agtagtctgg actacctgtt ttagcgtccg tacctgacat tttgaaacca
     8581 ccaaatggat ggtatccaac aactgctgaa gtacagcctc tgttaaggta taaattgcct
     8641 acatcaaatt cgtttaccgc tttaatccaa tgctcgcgat tatttgtaat cactgcacca
     8701 gttaaaccgt aatctgtatc atttgcaacc tcaattgctt catcaaaatc gttaactttc
     8761 acaaagccaa caactggacc aaaaatttct tcttgcatga ttctatcttt agatttaagt
     8821 cctgaaatga ttgttggttc tacaaagtaa ccttttgaat catcagtgcc gccaccttgt
     8881 tctaatttac cttcttcttt accaatctca atataatttt taatcttatc aaattgtttt
     8941 ttattaataa ctgggcccat atacgtattg tctacagtat tgcccaacgt taattctttt
     9001 gttaatttga ttgatttctc taatacttcg tcataaacgt ctttatgcac aattgcacgt
     9061 gaacatgctg aacatttttg accagaaaaa ccaaatgctg acgttacaat agcttctgct
     9121 gccatatctg tatcaatatt ttcatcaact acaatggcat ctttaccacc catttcagcg
     9181 ataacacgtt tcaagaagtt ttgaccttct tgaacaacgg cactacgttc ataaattcta
     9241 gtacctgtcg cacgtgatcc tgtaaatgta acgaaatgcg tatctttatg atcaactaag
     9301 taatcaccaa tttctttcgg atcaccagga acaaagttaa ctacgccttt tggtaatcct
     9361 gcttcttcta aaattcccat taatttataa gcgatataag gtgtatcctc agcaggtttc
     9421 aataacactg tattacctgc cacaactggt gctaaagttg taccagccat aatcgcaaac
     9481 gggaagttcc acggcggaat tgtaacacct gtaccaattg atttatagaa atatttattg
     9541 tgttcacctt cacgatcaag tactggctta ccttgagcca agtccatcat tgaacgtgca
     9601 tagtattcaa taaaatcaat accttcagct gcatcaccaa ctgcttcatc ccatggctta
     9661 cctgcttcat aaaccataat tgctgcaatt tccgcttttc gacgacgaat aattgccgaa
     9721 acacgtaaca taagctctgc acgatcattt gctgaccatg ttttccaaga tttataagct
     9781 tcgtttgctg ctttaaacgc atcttcaaca tcttgttttg ttgcctttga tgcatttgca
     9841 atcacttgtg atgtgtctgc aggattgatt gatttaattt tgtcatcttt gaaaatcttc
     9901 tctccattaa tcactaatgg tatgtcttga cctaattctt tttccacgtc tttcaatgct
     9961 ttcttaaaca tatccacatt ttcttggact gaaaaatcgt aaccaggttc atttttaaat
    10021 tctactacca tgtacactta ccccctataa attttgaaag tggtttaacc ctttgattta
    10081 atgatataac atcatttaaa ctcattttac tatgattaag gttagttttg caatcgcttt
    10141 catttttatg ctttatcact tattctcaag tattttgaaa ttgattggtt actttttaaa
    10201 atttatatgg gtcgcaactg ctactttatc gtttcgtcat ttaatgtttc ggatggtagg
    10261 tcattatcaa ttttacgaac gactttacaa gggtttccaa ccgctaagct gtgtggcgga
    10321 atatctttag tgacaacact accagcacca atcacactgc cttctccaat cgtcacccct
    10381 ggtaacacgg ctacatgacc gccaaaccaa gtattactgc caatatgaat gggtccggct
    10441 ttttcaaaac cttcatttct atgatggaaa ttaagtggat gtgtcgctgt gtagaatcca
    10501 caattaggtc ctataaaaac attatcgcca attgtaatct gtccaccatc cataaagtaa
    10561 caattggtgt ttacatagac attttttcct agtttaacgt tccaaccata atctgtatca
    10621 aaaggaatcg aaatacttac attgtctgtt gttgtttgaa ataattgatc aattaattcc
    10681 tttcttttat ttgtagcact cggtcttgta tgatttaatt caaagcaaat atctttcgct
    10741 cgtgcacgtt cattgattaa gtattgatca aagtttgcat cgtaccattt ttctgctaac
    10801 attttttctt tttcagtcat tacacctttc aactcctaat aacttattta cttgtttaaa
    10861 agttaatcaa ataaacattc gcctatgcaa ctaatacgct ataacattat gaaatcatga
    10921 ccttatcacc cttatctata caattctcgc atcaaatact gctaaagtag tagataaatt
    10981 caatactaca gacgcattca ttttttaatc tattaacgta caatgtgagt aagagaaata
    11041 taaaggagta tgatagcgat gagaatatta attacaggca cagttgctat cttaatcatt
    11101 ttaggtttgg tcaaaacgat acaagatcac gaaatgacaa acgacacgag tcgtcagttg
    11161 tcagacaaca aagatgatga taaagtcatc catcttaata attttaaaaa tttacatgcg
    11221 aaagaattta acccatctga tttcttttaa gtcacctaag aattgcaaat ccagaagtca
    11281 tttaagtttt acctttcatt catacatcct ttaatattaa ttacgacttc ttttatatag
    11341 atgctaaata gagagattgt tgtgcaatgt ttgcacggca atctctcttt ttctttttaa
    11401 aattggtaaa agtaaaacgc aacgattgac ttatatacct atagggggta cattagacgt
    11461 gtaacaatga atcacaggga ggcaataatg tggctaatac gaaaaaaaca acattagata
    11521 tcactggtat gacttgtgcc gcatgttcaa atcgtatcga aaagaaactg aataaacttg
    11581 atgacgttaa tgcccaagtg aatttaacta cagagaaagc aactgttgag tataaccctg
    11641 atcaacatga tgtccaagaa tttattaata cgattcaaca tttaggttac ggtgtcgctg
    11701 tagaaactgt cgaattagac attacaggta tgacttgtgc tgcatgctca agccgtattg
    11761 aaaaagtgtt aaataaaatg gacggtgttc aaaatgcaac ggtcaattta acaacagagc
    11821 aagctaaagt tgactattat cctgaagaaa cagatgctga taaacttgtc actcgcattc
    11881 aaaaattagg ttatgacgcg tctattaaag ataacaataa agatcaaacg tcacgcaaag
    11941 ctgaagcgct acaacataaa ttgattaagc ttatcatatc agcagtatta tctttaccac
    12001 tattaatgtt aatgtttgtg catcttttca atatgcatat accagcacta tttacgaatc
    12061 catggttcca atttatttta gctacacctg tacaatttat tattggatgg caattttatg
    12121 taggtgctta taaaaactta agaaatggtg gcgccaatat ggatgtactt gttgctgttg
    12181 gtacaagtgc agcatatttt tacagtattt atgaaatggt tcgttggcta aatggctcaa
    12241 caacgcaacc gcatttatac tttgaaacaa gcgccgtact aattacctta atcttattcg
    12301 gtaagtattt agaagctaga gcgaagtctc aaacaaccaa tgcgcttggc gaattattaa
    12361 gtttgcaagc taaagaagca cgcattttaa aagatggtaa tgaagtgatg attcctctaa
    12421 atgaagtaca tgttggagat acacttatcg ttaaaccagg tgaaaagata cctgttgatg
    12481 gcaaaattat taaaggtatg actgccatcg acgaatctat gttaacaggt gaatctatcc
    12541 ctgttgagaa gaatgttgat gatactgtaa ttggttcaac gatgaacaaa aacggtacta
    12601 ttactatgac agcaacaaaa gttggcgggg acactgcgtt ggcaaatatt attaaagttg
    12661 tcgaagaagc tcaaagttct aaagcgccga ttcaacgatt ggcagatatt atttctggtt
    12721 atttcgttcc tatcgttgtt ggtatcgcac tattaacatt tatcgtgtgg attactttag
    12781 ttacaccagg tacatttgaa cctgcacttg ttgcgagtat ttccgttctt gtcattgctt
    12841 gtccatgcgc attaggactt gctacaccaa cttctattat ggtaggtact ggtcgcgctg
    12901 ctgaaaatgg tattttattt aaaggtggcg agtttgttga acgcacacat caaattgata
    12961 ccatcgtttt agataagacg ggtaccatta caaatggtcg tccagtcgtg acagattatc
    13021 atggtgacaa tcaaacgcta caactacttg ctactgctga aaaagattct gaacacccat
    13081 tggcagaagc cattgtcaat tatgcaaaag aaaagcaatt aacattaact gagacgacaa
    13141 catttaaagc agtacctgga catggtattg aagcaacgat tgatcatcac catatattgg
    13201 ttggtaaccg taaattaatg gctgacaatg atattagctt gcctaagcat atttctgatg
    13261 atttaacata ttatgaacga gatggtaaaa ctgctatgct cattgctgtt aattattcat
    13321 taactggtat catcgcagtg gcagatactg taaaagatca tgccaaagat gcaataaaac
    13381 aattgcatga tatgggcatt gaagttgcca tgttaactgg cgataataaa aacactgctc
    13441 aagccattgc aaaacaagta ggcatagata ctgttattgc agatatttta ccagaagaaa
    13501 aagctgcaca aattgcgaaa ctacagcaac aaggtaagaa ggttgcgatg gttggtgacg
    13561 gtgtaaatga tgcacctgca ttagttaaag ctgatatcgg tatcgccatt ggtacaggta
    13621 cagaagttgc cattgaagca gctgatatta ctattcttgg tggcgacttg atgcttattc
    13681 ctaaagccat ttatgcaagt aaagcaacca ttcgtaatat tcgtcaaaat ctattttggg
    13741 cattcggcta taatattgcc ggtatcccta tagctgcatt gggcttactt gcgccatggg
    13801 ttgctggtgc tgcaatggca ctaagttcag taagtgttgt cacaaacgca cttagattga
    13861 aaaagatgcg attagaacca cgccgtaaag atgcctagat tccttaataa tgaaggattc
    13921 gttggtgatt ctgagatagg ctagtgattg gctctataat gtcgcggttt acagttggat
    13981 tttcgctcca actgcatata tagttacact tttcgcttgg cgaattagtg tatcttacct
    14041 aatagctccg cctattaggt tccatcatta ttataaataa taagtacact acggtttaca
    14101 gttggatctt cgctccaact gcataagagc cactaattat tataaataat aagtacacta
    14161 cggtttacag ttggtccttc gctccaactg cataagagcc actaattatt ataaataata
    14221 agtggctcta aaaggaggtg aatgctatgt cacaagaaat tttaaatgtt gaaggtatga
    14281 gctgtggtca ctgcaaaagt gctgttgaat ctgcattaaa taatattgac ggtgtcactt
    14341 cagctgacgt taaccttgaa aatggtcaag taagtgttca atatgatgac agtaaagttg
    14401 ctgtatctca aatgaaagac gcaattgaag atcaaggtta cgatgtcgtt taattaggca
    14461 atattcaacg tcatcaacac caaattaaaa aatcgaactg atgagaatcc caacaatcca
    14521 aattatctca tcagttcgat ttttaattta ctcgtaacct agtatctcca gtctgcaata
    14581 catctaatgt tgcatctaat gcatcgacaa ttagattttt aactgcagct tcagtataaa
    14641 acgcaatatg tggtgttaat atgacatctt ccctgtcaat caacgattct aacaatggat
    14701 cgttcagtgt tttgcccctt tgatcacttg ggaaaagttt gcgttcaaat tcatacgtat
    14761 caagtgctgc acctttaatc acaccattgt ctaatgcgtc taataacgcc ttagtatcta
    14821 ctaaagaacc tctcgcacaa ttgacaaata ctgcgccctt tttaaaatgt ttaaataatt
    14881 cagcattaaa tagataatga ttatatttcg ttgcaggtac atgtaatgtc acgatatcag
    14941 caccttcaac cgcttcctca atcgtatctt tgtaatcgac atacgttgca attttagcat
    15001 taggaaacgg gtcgtatgcg accacatcac tttgataacc attggcaaat atatcggcta
    15061 ctacacggcc aattcgacct gtaccaataa cagctacttt taaatcttta atggatttcg
    15121 ataaaatagt aggttcccat ctaaaatcat gctcccgcac tttcgtttga atttgattaa
    15181 aatgacgaac cacattaata gcctggttca cagcaaactc cgcaattgaa ttcggagagt
    15241 atgacggcac atttgacaca atcaagttat acttgtttgc taactccaaa tcatatgtat
    15301 caaatccagc actacgttgt gcgatttgtt taatatcaag ttcatttaat cgtttataaa
    15361 catgctctga taatggtatt tgttgtgata gcgataagcc atcataacca gcgacacctt
    15421 caacattgtc atcagttaat gcttctttag taatatctac ctcaacatga tgtttctctg
    15481 cccacgcctt gatataaggc atatcttcat cacgtacact catgatttta atttttgtca
    15541 ttttaacatc acccttaact ttattattca tataaatatg ctagttctgt taatcttatt
    15601 gcagcttcgt ctaatttctg gtcatctaac gccaatgaaa ttctcacata acgattacca
    15661 ttctctccaa atggtttccc tggagcaaca agtattgact tctcttgcac taaaaattgc
    15721 tcaaattgct cgctgtcata accaggcggt gtttccaacc atacatatat gccaccttta
    15781 gcatgaacaa atggcaaatc agcttttgca agcatggctt cgaatcggtc acgacgtgtt
    15841 ttaaatacat tgctttgttc ttctaaaaaa tcatcataat gattcaaagc atatattgcg
    15901 gcatcttgta atgcaccaaa catcccagca tttgtgtgcg tttggtactt tttcaaagct
    15961 tgaatcatat ctttattacc aactgcaaaa ccgactctaa aacctgacat gttataacct
    16021 ttagacaagg aataaatttc aatcgcaaca tctttcccat tttccgaagc aagtatacta
    16081 ggatttttag cgtcgaaacc gaaagcacca taagcaaaat catgcacgat tttagtgtct
    16141 gtatctttaa atttagctat cgcttcatca aaaacttctt tcgtagctgt cgatccagtt
    16201 ggattatttg gatacgttaa ataaatgagt tttgttttat ctattatttg tgaatcaact
    16261 gtggaccaat ctggcaaata atgtggcggt tctaaattaa gcgggacagg cttgccatca
    16321 gctaaaagta cacctgctaa ataatccgtg tagcctggat caggtagtaa tacatagtct
    16381 cctggattga taacacatgt tggtactgcc actaatccat tttttgtacc atataaaatg
    16441 cacacttcat cttctttatc taacgtcaca ttatattgtc tttgataaaa atctacaata
    16501 gcttgcttga acgcttcttt accatgaaaa gcaccatatt tttgattttc aggaatagtt
    16561 agtgcttgtt gaaaatgatc aataatacct tgtggcgtag gcccatcagg gattccaact
    16621 gccatattaa ttaatggcaa tggtccatgt tcgattttac gtcccatcgt tttcccgaaa
    16681 taactatcag ggatatttgc taatttgtta gagatcatca aattcctcct ctatcattaa
    16741 acatagcctg ggcgactatc ataatcctaa caacttgtat cactctcatt tagatggtta
    16801 caatgacatc gccattcacc gttatgttca acagaactta tgacacacgt tgtattgaat
    16861 gaatttattt tcattttagg taggtataat attattgtca atattaggaa ttttcagatt
    16921 aatatacact caatcattat gattcaactg ccatgcatat ccgcatgcgc aaccagttag
    16981 atatgcttat ataaagtata acgcccatca aggtacgtat tcaaacgtga accttaacag
    17041 gcgtcattca ttgttaaata aaacttctta agcacatact tatttcacta tgccttttac
    17101 gttcccctta tacttttctc acatctttct cttagactac tcccttacac gccttgctca
    17161 atatctttaa tcatttcatc tacagttatt ttcgcactcg ttaagacaat aggaacgcct
    17221 gcacctggat gcgtacttgc acctgcaaaa tataaatctt tataatctcg cgatacattt
    17281 tgtggacgat aataattact ttgcgctaaa gttggcatta aaccgaatgc cgaaccaaat
    17341 ttcgcatgat acgtttgctc aaaatcattt ggcgtaaaga ttgtttctga aacaatatgc
    17401 gattttatat cttcaaatac ttcaatcgtt gctaatttac gataaataat ttcctttatt
    17461 tgttgcgtca aagcttcatc tgaccaatcg attccgctac ctgttttaag ttccggcgtc
    17521 ggcattagca cataaatacc agttttgcct tctggcgcaa gtgatttatc agcgaccgct
    17581 ggtacataca cataaataga aggatcatat gataaacgtc cctcaaatat ttcttcaata
    17641 ttgcctctaa agtcatctga aaaaataaca ttatgaagtc tcacttgatc tgtcacatca
    17701 atatctatac cgatatacat taaaaatgct gaacaagagt aatctaagtc tgcaatttta
    17761 tgtggtggat actttttaat aggtgcaaaa tctggcatta atgattccgc aacactaggg
    17821 aaatcagccg tacataaaat tttatcaaat tttcttatgt caccattcac ttttatcgca
    17881 tcggcccgtt tgaatttagg atcaataata atttgctcaa tttcagcatt tagttcaata
    17941 ttaacgccta agtctttatt taattgcgct agcccttgag ccatgccata cataccgcct
    18001 ttaataaaat gcacaccaaa catcatttca atcataggaa taattgaata tagtgacggg
    18061 cctcgttttg gatcaattcc tatgtataac gtttgaaacg ctaaaagctt ttgtatcttt
    18121 tcgttatcaa tataatgttc aattagctga tctgcatgat ttaacgtttt taacttagca
    18181 ccttgcacaa gtgacgtcat attataaaag tcactcggtt tgcgatacgt tctttctaag
    18241 aaatagcgac gtgcaatttc atatttttta taaacatccg ttaaaaagga cataaaacca
    18301 tgcgttgaac caggttctat actttctagc atttgctgta attcagctaa atctgtaggc
    18361 accgttatac gatcatcgtg gtcaaaatac acatcgtaaa tataacgtaa ttgtctcaat
    18421 tcaatataat cttcataatt tttaccacac gctgtaaaaa catctttata aacatctggc
    18481 atcatgacaa ttgtgggacc catatcaaat gtaaagccgt ctttctttaa ttgattcata
    18541 cgcccgccta cattattatt tttttcaaat atcgtcactt catgaccttg agaagcaata
    18601 cgggctgccg ctgctaatcc tgtgacacct gcaccaatta ctgcaatctt cattattcaa
    18661 ccacctatat tctatgatat ttactattta tttcatgaaa caactttgcc tttttccgct
    18721 tatccacaaa aacacgttca tgtaatgtat agttagcctg tctcacttcg tccagtattt
    18781 caatatatat acgtgctgct aattctatga ttggttgtgc ttcaatacta aatactttga
    18841 tttgatccat aacatcttga aaatcttttt ctgcgatagc tgcataatat tcccataagt
    18901 caatataatg attattaaca ccattttggt acacttcagc aatatcaact tcatattgct
    18961 ttaatcgttg cttactaaaa tatatccgtt cattgtcaaa atcttcaccg acatctctta
    19021 atatattaat caattgcaac gattcaccaa gtcttcttgc gacatcgtat gtctgatgtg
    19081 tttcatgatc acttaaaatc ggcgtcaata cttcacctac tgtaccagca acaccataac
    19141 aatatccgaa taattcagcg tccgtttcaa acattgtaaa atgttgatct ttatatacag
    19201 tatcaatgag attatagaaa gattgaaagg cgatattttt atgttgtgca acatgctgaa
    19261 gtgccatcat tattctacga tcactttgaa agtgatgatg ttcatatggg tatttttcaa
    19321 tagattgtat atcttctttt atttgattta aaaattgaat atcgccataa acatctatac
    19381 tgtcatcaat tttacgacac acagcataaa ttgcccaaac cgcttttctt tgatcttctg
    19441 gtaacaagtc aaaagcgtaa gaaaagcttt ttgaatgttt cttcatgatt ttatgacaat
    19501 atttaaaatt catatccatc attgtcatac tagtcctcct atattgaaat gccattacct
    19561 tgatttattg ttctttagat atagaatatt gacgaccttt ccattcaact acaccatacc
    19621 gatgcgtttg tttccaagat tggatgaaaa ttttagtaaa aaacataaat aacaatggat
    19681 gacataccat taataaatta gaaaattggc cgacccttcg atgcagataa ataaattgtg
    19741 tagtatagct caagtaaagt gctaccattt ttcttacaga catttggcga tattttaaac
    19801 taagacatag ccctaaaata ctcgctatag aaccaaacaa ccacaacaca attgctgtca
    19861 tgatcttagg ctttgtgcca cctgccccag ttgacaaatg ctttgtccat ccttcttgta
    19921 atgactgaaa tccttcttga tacatgcgaa atgcaacaaa tggaaaccct tcataaactg
    19981 ttacgggcaa tgattgtgaa gtatatgcac ttcctaaagc aaatccttca ataatatgac
    20041 ggtttgcact tttatgacct ccagttgcat aataatcttc tttatttgtt aatgtcaccg
    20101 gtccaaatgc atgctggtta gtccgaccgt cgtctaaggt agaaaataca ttcataccaa
    20161 cgactgtcat taaattaaat atcgctgaaa acccttcgta gaaacgcttt gttatatgat
    20221 aaggctgtac gcttaacaat cctttttcac cttgtaattg atactgatta atcaacgttt
    20281 caacagcatc tttccttaag aaagttacat cagcatctac aaaggcaata cgattcgtac
    20341 atgcatgcgt cacaccttga taacaagcat gtgatttccc ataccatttg ccgtcagtat
    20401 catcaacaac atctactaca gtcgcaccat atgaacgtgc tacacgtgct gtttcatctg
    20461 tcgatccgtc attcataaca atgacatcta ctggaacttg ctgttgtatt atcgaatgta
    20521 gtaaatgacc tattcttttt tcttcgtttc tagccggaat aataattgtt aatgctttat
    20581 gattgaagtt cagcgttttc gcctttagct gatgtctacg attaaatatc aatgcaccac
    20641 acgccataga catggtcact attactgtta atattcgtga taaccatttc atagatatca
    20701 cttcctattc ttcgcttctc gcgcgtttat tttgtctgcg acttgttgcc cacttaatgt
    20761 aaccattggt atgccaccac caggatttac tgatccacct acaaagtaca agttttcaaa
    20821 atactgactt tctttaggaa atttaaatcc tttgtttttc tttttatctg ctacaacacc
    20881 atatattgca ccacgattag aacgataatt tttttcaata tcctccggtg tccaaacatc
    20941 ttcataaata atgtgttttc ttaaatcagt aagtcccatt ttttctaatt tatccaaaat
    21001 tttatcccta aacttcgcat aatcttcagt tgtaaaaggc tgatcttgaa tatatggaat
    21061 atgtggtaag actttgatat tttcataacc tactggcgct tgtgtgtgat cagttttatt
    21121 cgtatttact agataaatgg tcggatcatc tggcaatacc ttttcatgaa aaacttgttg
    21181 ataattgaga taagcatttt ccgtaaaaaa gaaattatga tgtgctaatt gcgggtattg
    21241 gcaagcaaca cctaaatgca tcacatatcc tgagcttgcc ggctcaaatt ccctctctaa
    21301 tttgtttaat cgttgagtat caaggtgaat taaatattta taagtaggta tgacttccat
    21361 atttgaaata atataatctg cctttacaaa ctcacctgta tctaatctga cacccgtcac
    21421 acgtctttga tatgttttaa tattgtccac acgtgcacct gtatgaattg tgacaccttc
    21481 ttcacgcgct agcttttcca aggcattggc taaatgatgg attccacctt ctacatacca
    21541 aaggccttgc tcttgttgca tatggaataa catagataat acagctggcg catcgtatga
    21601 tgaagaacct acatatttga taaaatagcc taacatttgt cgcaagtatg gattcgagat
    21661 acgcttgtct atggcctgtt gcatagtatg tacataatca taattaataa gagcatttaa
    21721 tggcccatga aatttaataa tttgagatag tgtatctaaa ccatagttaa aatacccttt
    21781 ttcagtaata cgatcgattc gtcttgtata attcaaataa ttttgcagtt cctctatatc
    21841 ctgtttcgac aatatcgcat tatgctgacc tgtttcttta ataccttcat acaaatcgat
    21901 agtcgttcca tctggaaaaa agctacgcca ttgatgtggc aatcgcttga ttgtaacgta
    21961 gtctgacatt tgcttcttgc tatattcgaa taatttttca aaaatataag gcatcgttaa
    22021 aatagatgga cctaaatcaa agccaaagcc atctgattca tgacgattca ctttgcctcc
    22081 tatatgatta ttttgttcat ataatgagac cgaatagcca ctttgtgcca ttcgaattgc
    22141 tgcagaaatc ccacctaagc caccaccaat aacgatgata tgtttagtca tgacgttcac
    22201 ctcttcgttt taatatgcgt agtaactgcg ttaatctcgg tcgattatag cgttgcacaa
    22261 taatgattgg aacattagct atcattgcat agaaaatatt tatatacttt actaaacgag
    22321 ggcctttatt gaatatgatg actggaagta tcgatatcca atgaatcaac tctgccctct
    22381 ttgtctctat aatcattttt tcaatttcat cagtattgat tgtcattaaa tgacgctgat
    22441 catatatatt ttgattaagc tgatgaccat ctaaaatctt atgtttccat tttcttacgt
    22501 aaaaccattt gttccaaaat tttccatgct tctcaaagtt aaatgattta aatattatgt
    22561 acttctgacg aaaatactta tcaggaattc ttgtacctaa atttgctata tttagttgaa
    22621 caattaacca atacatacta caaaaaaatg ctgttttaat atattttttc atggttttca
    22681 tctaaattga atcactctca atcatactga catagtataa attatcactt tattacttag
    22741 ttccctaaag atcagtgaat aaaccgctct catttatatt tatataaaac aaaaacgcca
    22801 atactctcat gttagagaat attgacgtca tttgatttac atttgattaa tgaatgaaat
    22861 tatatgaacc tgcttggttt gctgaaattg tacgagacgt aaccacacca gcaccatgtc
    22921 catagttcat ttctgaaaca cgaacagaac cgttgctgtt aacgccttca acgtaagcaa
    22981 catgaccgta atagccttgt gttgtttgca tgatagcacc aacttttggt gtattgttca
    23041 ctgtatagcc agatgatgca gctgcgttag cccaattact tgcgttaccc catgttgaac
    23101 caattttccc accaacacga tcaaatacat aataagtaca ttgtcctgaa gtatataagt
    23161 tacttcctga tgcataacca ttagaaattg aacgaccatt tgaagatggc gctgcagttg
    23221 tagtcacatg aacattatta cttgttgtgc tataacttgc acctgaacca ccagtataat
    23281 agttatttgt tgctgtttga ctattgtttg tatagttatt gtattgatat gaatgattat
    23341 agtagttatt gtaagatgca ttgttataac tataagtatt gtagttgttg tagtagtatg
    23401 tgttgttttg cgttaattga cttggattcc aatttcctgt ccaagtgtaa tgataattac
    23461 cttgtgcatc aattgtatac gtatagctgt atgattgagc gtcattagaa ttatatccat
    23521 tgttattttg ttctgcggct tgtgcatcat gtcctgcaaa tgcgatagtg gcaagtcctg
    23581 ctgtagcgat tgtagctgta acgatttttt tcataattaa atatcctccc aataatcaaa
    23641 acactctata catatagtca ttagcgactc aattatttta aattttatat tttttatttt
    23701 tttagtatgc aaataattat aacttttata gttttgcgac aaaacaaact ataacagaga
    23761 ttaggtgtct tgcgggcttt gttatcataa tgtaatctgt tcctaataaa gcttcattga
    23821 atgtaatata ccaattagaa tgtttacaac actttttctg caccgttacc atgggatttg
    23881 aaaataaaaa agcactaaac ttttaatgaa atacatcatt tttgataata aatatcagaa
    23941 gtaatgtaat atttcaaaag tttagtgcat caaattattt cttatttgta gcatgtgttt
    24001 ccatcgtttt tacaatcaaa tcagtcagcg ctttactacc tgcatattct aagtgaatac
    24061 cgtcatatgc aaagtattcc ggatgacctg cagaagcttt ataccaatcg actagatgta
    24121 cattagagcg cttttcagct gcctcataaa ttaatttatt tattctacct tcataatctc
    24181 taggtactct aatagaaact aaataaatgt ctgcttttcc aaaactatcc aatagttcat
    24241 ttaattgatc tttcgtaaat gccccatttg taccaagctc tactacaact ttttgacctt
    24301 ttttagcata gtctttatat tgcgatttca caattggtgt agcatcaacg agttgccgtc
    24361 caactttacc atcaatttgt gcatttggta ttttcttagt aaagacatta ccaatatcca
    24421 ccatgaccga gtcaccaatt aatagtggtg atgacttttt aatatcctct ttgctattcg
    24481 ctgtctgctt atcctcttgc ttatcttttt taacgacttt tttcttcgtt gttttaaatt
    24541 ccgtcttctt ctctttcttc tcggcttcat gttgtttgcc aagtgcatca aactgtccac
    24601 tgagcacaac gatagacgga accaataata ggataactaa cactgttcta gcaaattgcc
    24661 ccttcttttt aggtaaaaat gcaaaagctt taaatccttt tttacgtata ggtgtttcaa
    24721 taaagcgata cgaaatttca gccattaacg ctgttaacaa aatttctata atataaacgt
    24781 ataccggtat ttgtccttgt acgtaataac tgttcacaaa aacaatgata ggataatgcc
    24841 ataaatataa gctatatgat cgtttaccta taattagtaa aggtttcata cttaaaaatt
    24901 tagcaaataa actagaagga tgtaccgcaa ttgcaataat gaataaagtt gcaaatgata
    24961 taatgtaaaa tcctccatta tagatccatt gatcttggtc gccaactata aagaacaaag
    25021 tcattagaac cgcaaaacca gatatcccta taatatctaa tgatacgaca atctttttag
    25081 aaatatcttt tttcaaagca aacggaggcc aaataaatgc taatatacaa ccaagcaata
    25141 aagtttgcag tcgtgtatct gtcccaaaat acacacgtga attatctcca gtgatgaaat
    25201 gaatcactat cataagtcct aaagaaatca acgatacaat aaatagcgtt tgaataatat
    25261 ttctcggttt aaatctatgt aataagaacg tgataaccaa tgggaaaagc aagtaaaatt
    25321 gttcttcaat ggctaaagac cataaatgtt tgagtggttc aatagcaaat tggttaaaat
    25381 aatctacatt ctgtgagatg taccaccagt ttgaaacata gaatatagct gcaatagcat
    25441 ctcgtttcat ttgtataatc aattccggtt taaatatcag tgtgaacgta agcacgacac
    25501 aaattaaaaa caacactgcc ggaatgagtc gtttcaatcg tcgcttccaa aactctagca
    25561 aatcaatttt ttgcgtccga taatactcac ttatcaacaa acttgttatt aaataacctg
    25621 aaataacgaa gaatgtatct actcctaaaa agcccccact taaccattgt gcattcaagt
    25681 gataaataat gattcctata actgcgaatg ccctcaaccc atctaatccg ggtaagtatc
    25741 gcggggaata cattttttct aaacgtttaa agtcttttgt atccatgtta ataaacgccc
    25801 catttatttt tctctatttt gtagcatatc acaatatttt tgaaaataaa atattgcact
    25861 gattttcatt aattgattta acccttaatt aagatagttt taaatttttt attaagtaga
    25921 aaacaattat tacagttaat ttcattactg caaaccacat ataaatttgt cgattttact
    25981 acataacata gattatcata gattcttgaa tttttagcaa aataactgtt attttcatta
    26041 tatttttaca aaaaaaaggt tcgttttata ttttatgcct cttactgtaa cagaatcatt
    26101 aagatatgct attcgaatat actttttcaa aatttatata atgaataaat taacatgtat
    26161 tgaaaaaaaa gcgaaatgca gcctatcctc taatgtaaac caaacgatat atctcgtcag
    26221 actttatatt taaacgctat gtgtcacttt taaaatgaat attactaaga ttgtcatatc
    26281 aattattatt gcatcgaatt aatcttttaa atttctgtaa taaaacctat tatctacatc
    26341 taatttagga aaactacttg gtaaatctcc tcgcttaatt tcacggaagc cattattaga
    26401 ataaaaatac tgtgcactaa taaatttatc aattgttcct aaataaatac catcgatatt
    26461 ttgttcttta catgtcatta taactttatc taaaagtttt ttacctattt ttaaattcct
    26521 ataaccttta tcaacaaaca tttttttaag tgcagacata ttattatcta gtctaatcaa
    26581 acctatagta ccaacaatat tttgatgatt gtttattgca agccaaaatt gccctccatt
    26641 attcaaatag ttatgttcga tgttctccaa atcaggttga tcatctctat caatttttat
    26701 attaaattca tttttttgaa tcgataaaat aaactcgatt agctcttcct tataagacct
    26761 attatattca attatgttta tagccatttt tatctccttt ttcatttaat ttaattataa
    26821 aatgtgcgtt tagtttgtat ctagtgtact cagtacagcc tcaaatgaag tttcattcca
    26881 cttggcactt aataaagaca agtattttag cagtaataca ataaagtcca ataaatttcc
    26941 ctaacttcaa tatccacttt ttaaaaaatg tatttttaat taataaaaaa actctcccca
    27001 atttctatgg gaagagctat atatttaatg tctaaacatt acttttattt attatgaagg
    27061 aattagaatc cccaagcacc taaaccttgt gctttgtatg ctttaacagc tgcgttgatt
    27121 tgttggtcaa cagtgtttgt tggaccccaa cctggcatag tttggaataa acctgaagca
    27181 cctgatgggt tgtaagcatt tacttgacca tttgattcac gagcgatgat tgcagcccat
    27241 gtagaagctg aaacaccagt acgttgagcc atgatttgag ctgctgatga accagtagca
    27301 cctgcagtat taccattgct taatctcact gaacttgaag tagttgaagt gctgtagtta
    27361 tggtaagttg gagctgaaac agcttcaacg tttgagttac ttgattgtgc attgtagctt
    27421 actgattgta catttgaacc ttggttgtat gaagtagtgt agtctgcacc tgcaacgttt
    27481 gagaaaccag cagtttgacc attagctgct tcatagctcc atgaccatgt agtaccattt
    27541 gaagtgaagt tatattggaa accatctttt acaaagtgga tgtcatatgc accatctttg
    27601 attggagctg catttaattg atcttggtga ttgtgtgcta agtcaactaa gtgtgcttga
    27661 tcaacgttta cttcagcagc gtgtgcttga tgtcctgtac ctgctgcgta acctgttaca
    27721 cctaatgcca ctgctaatga tgatgccata attgtctttt tcatagtaaa aaatcctcca
    27781 gtaataattg taagtttatg tttttagtaa ttatattttg aatttgaatg tcgtagtgca
    27841 agtttaaatt gtcttttatt tctttcaacg gtactcacta tatcacaaaa aaccagccag
    27901 taaattacac tttctttaca aaacattaca atatcaagtt ttatttgtaa tgttgaaata
    27961 tggctgtttt atactgtaat gtgaaatatg tgccctttag aatccaataa acccttgaaa
    28021 tagtctttaa cgcataagat ttttactata tttagctcaa ctattacagc tttcgtaata
    28081 ttacagattg tatttttgtt acatagctgt aatatatctg acatgtaact tctcttattt
    28141 tcgtacatta aacgcaatta aaaagcaatc aacacatatg tttctacaca tgtattgatt
    28201 gctattattg ttgtatatcc aaagttttaa aacacacatc ttttgtgaat tgtcttatct
    28261 tttataagca caaataaact gcaccttaat tatattgcta aactctatta tcgtaattca
    28321 caataacatt aaataatttt tgatctcata ttttcaaaaa acatactgtt attatcccat
    28381 gaatttaaaa atatcattag tatataaacg aaacacttta cgataaatga tatctgcaag
    28441 ccaagctgtt acaaatggta caacaaagaa cgctactaca attagtaaga cactcaacca
    28501 agcagaatca acctccataa atttaaatgc attaatcggt cctaccattc ctataaaacc
    28561 aaatccagct gactctttcg ttccatgaat acctactaat gctgatacca aacctgatac
    28621 aatggctgtc gttaatattg gtaacataag aattggatat ttcaccatat taggtatcat
    28681 cattttaacg cctccaaaga agacggataa cggcacccct aaacgattca ctttacttgt
    28741 accaattatc aatactgctt cagtcgcgga gataccaatt gaagctgatc cagctgctaa
    28801 acctgaaata cctatcgcaa aggcaatggc cacagttgat agtggcgaaa taataataag
    28861 actaaatacc attgaaatca aaatactcat gacaatcggt tgtaattctg taaaaccatt
    28921 aaccatatta ccgatggctg ttgtaatcat tttcgtatac ggcaatatta aaacaccaat
    28981 tgcacctgaa ataccgccaa caactgttgg gaatacaatc aatgccatac tacctacgcg
    29041 atgttgaata agtaaaatga acaacactgc aatcgctgct gtaatcattg tattaattaa
    29101 atcaccaata cccgtaatca tccaagcacc atttttaaac tgcgctgcac cgcttcctac
    29161 atatgctgca ctcgccacaa cagcaattgc taatggcgat aggtcaaatt tcatggcaac
    29221 caatgcacca atcaaagcag gtactgtaaa ttgaattgca acgacaacgc ctaataacgt
    29281 tttaaaaatc ggatgataat ccataaagta tttaaaaatt tctccaagta tcgcattagg
    29341 aactaaaccc gcaacaatac ctatggcgac acctgataaa actctaaata taaaatcttt
    29401 gggtgtaatt gttttaattg atgtcataat atcatccttc catttatgta tatacatctt
    29461 tatgcaaata ataaagagcc ttaagttata agctgccact agcttaaatt ctaagatgtg
    29521 catgccgatg ttgttatatt taggctagca gtaacatcta taactcaaga ctatgaaaaa
    29581 tagtatatcg caaaattctg aatttttaga taaagaaatt ggcaattttt caaacatatt
    29641 gttacaatac acttttattt tatcttcatt tttaaaatcc attaatacaa tagaagaaag
    29701 acattcaaat gcttaccaaa aaggtacatt atttgttagg agcgtatcag cacttatata
    29761 tcatcaacac aattgacaat ataatagaag atactgataa taagtgttaa aacaacagat
    29821 gttaggtagt gaacaaatga tggaaagtaa atccatagat ccaagaatcg ttagaaccaa
    29881 acaattgctt gtcgatgctt ttcttaaaat ttctagagaa aagaaattat ctcaaattac
    29941 agttaaagat atcactgata ttgctacgct aaatcgtgca acattttacg ctcatttcac
    30001 tgataaagaa gacctcctag actacacatt atctgtaacc attttaaaag acttgaatga
    30061 taatttgagc atttctaatg tcattaatga aaaggttctg cgtaatattt tcatttcaat
    30121 tgcgagttat atcaaagatg ctgcaaagtc ttgcgaatta aatagtgaag cattttgcaa
    30181 caaagcacat caacgtatta ataatgaatt agaagatatt tttgcgatta tgttagaaaa
    30241 cagctatccg gagcatcaac gagatatcat tgtaaatagt gcgagttttt tagcagctgg
    30301 tatctcaggc ttagcattac attggtttaa cacgagtcaa gagacagccg atgtgtttat
    30361 cgatcgcaac cttccatttt taattcatca tatagcacat ttttaataaa acttggtatt
    30421 tagtcatgca tcttgaaatc actatgtgac ttaggttcat acttgtacac acaataaaat
    30481 ttaacgtatt acgattgatt agccgtgtct aggacataaa tcaacgtcct atactctaca
    30541 atgtcatatt agcagtcgtt aactgaatga aaataagctt gtcattaaaa catatagatt
    30601 ttagtgacaa gcatttttgt ttttgcgtac ttaaacaaca cttcagacaa tatgttgttt
    30661 aggcaacaaa tgatatgtgc gtgtttattg gcaaacgtac aacatagtag tatagtatgt
    30721 ctaaacaaca catgttgcat agttgatata cgttgtttaa atactaagat aggagggatt
    30781 gacgtgagcg agacagatga acctcagggg tttgaacgca cgcataatat attaaatatt
    30841 aatcagaata ggcagagtct aatgacattc attataagta aattgaagtc gacgttgaag
    30901 caacgcataa taaatgctcg tggtaaaaag cgaatcgact atcgactgtc gtataacttt
    30961 tacattacgt attatgatac tgtagaaatc aagaaaatcg actgtgaata tacctatgct
    31021 atgcccattg caattttaat aagacacacg atgtcattcg acaatgctca tttctttgct
    31081 cagttacgtc atcctgtctt ataaaacaac attgcagaca tgtatatcaa acgacacttc
    31141 aataacatca ctttgccatc gtactactat taaaatcgtg tctcaaaccc cttattttaa
    31201 ttccaaaaat ctgctggtca aaagaccgag aaactaaaaa cattactgga tgtgttgata
    31261 aattatctta tagaaataaa ctcaaaatat atcaacactt gattctaagg aggatatgac
    31321 aatatgaaaa ttttagatag aattaatgaa cttgcaaata aagaaaaagt ccaaccactt
    31381 actgtagctg aaaaacaaga acaacatgca ttgcgtcaag actacttaag catgatccga
    31441 ggacaagtat taacaacatt ttccacaata aaagtggttg atccaatcgg tcaggatgtc
    31501 acaccagata aagtgtatga tcttcgccaa caatacggtt atattcaaaa ttaatatttg
    31561 ctcacgaggt attgcactta aggtgccaac tgacctcata aacaaagccc atactgattg
    31621 aagacactaa tgtgtccacc atgtctctta cgacatgtgc acattaaacc ttcatctttg
    31681 tatgggcttt ttatttattc ttttgagaat ttcattttag cagaccaaaa aattaaaatg
    31741 gtgtccatca agatctttga acattgcacc ataaaaccct tggcttactg ttggttcttg
    31801 aacgactgtg ccacccgctt ctttcacttt attcaccaac tggtcaactt cttcatttgt
    31861 gttcacacct agagaaatca tcacttcatt tggttcagta ttaaggcttt gctgacttac
    31921 attttgaaaa tgcttgtttt ctattaaaat tacggttgtt tgacctattt gaatgccgac
    31981 cattttatct aacatttgtg ggtttctatt tattttaaat cctaacgctt tataaaactg
    32041 tgcgctcttt tctaaatctt gcacatgcaa attaaaccac attgattgaa tcatgattgc
    32101 accccattca ttacttatta tagttttgga ctttaagcca atcacttaat gataatcttg
    32161 ttggatttat ttcagccatt aattcaaagt ctacttcata acctttttct tccaaccatt
    32221 gcttttctgc aacaccacta acaaattctc cttctataac agtagattta cctgtcactt
    32281 cactaaaaat tgttgctgct tcacttaatg taacttcatc ggaaccaatc tctattgatt
    32341 gatgcgtaaa gctttgtgga tgtgcaaaaa tatacgatgc aattttagct atatcaatag
    32401 aagaaatcat tgtgaatttt atattcggat taataaattc tggtaatgta atacgttcat
    32461 cttcgacttt agcaatgcgt aaaaaattat ccataaagaa tgatggtttg ataactgttg
    32521 catttatatt agattccatt aatctatttt ctatttttgc tagtacttca aagtgtgggc
    32581 cagttcgatt tcgattaacc cctcccgcag tactatacac aatatgttga atattttctt
    32641 gctcagctat ttcaattatc ttcatacctt gtcttaattc ttcgctaaca tcatctttaa
    32701 cgattggctg aatactgtat aagccatact tacctttcat cgctgattgc aaactaacat
    32761 tatcactcag atcaccttca acgattgata aatgcggatg tcctatgtct gaaagtttac
    32821 gattattctt atttctagtt aatgcactta cataccatcc atcctctaac aactgtttta
    32881 caactgcatt accttgcttc cctgttgcgc ctattactaa aatatctttc attttagatc
    32941 cactcctctt ttatcttaaa aaccaatagt cctctttttc accttttaaa tacgtatatg
    33001 gttctggtgt ttgtccattt cctttaatat tgtcgtttat tacatctctg acatcccata
    33061 ctaaatgaat aaatcgttgt aattgatgac taaaaatgcc tagtggcgct gtcatagcta
    33121 atactttttc cacatcttct gtagtgacat tcagtttttc agcaatcatt tgaaccgata
    33181 aggtagattc ttcaaaatgt gcttgcaact cttcgcacgt aatgcgtctt tgctctttat
    33241 ttaaagacat atgcaacact cttttctttt ttaaatattt acagcacttt gcaaattcat
    33301 tgctatcttt cttaacacgt tttataataa acggagagtt atccgtttgt caattttaga
    33361 gaattttaag taggtgtaat catgcgaaaa gatgcaaaag agaataggca acgaattgaa
    33421 gaaatagcgc acaaactttt tgatgaagaa ggcgttgaaa acattagtat gaatcgtatt
    33481 gctaaagaac taggtatcgg tatggggaca ttgtatcgac atttcaaaga taaaagcgat
    33541 ttatgctact acgtcataca aagagacctt gatattttta tcactcattt taagcaaatt
    33601 aaagatgact atcattctaa ttatgaggtt atgcaagtgt cgttagatta tcttttacaa
    33661 tttaaaatcg ataataaggc actactgcaa tgtattgaag caggcaacaa taaattacgt
    33721 ttttaccaaa gcgcatttta tcaagaatta tttgattttt attatgactt atttaaaagc
    33781 gatgacgaca cttacacgaa gttcaaaaca gatatgctgt tgcaatcatt atcaactagt
    33841 gtatttgcct tccaaataga gcatcgccat atttcaattg aagcgtacag aaattattta
    33901 ttaaacattt atttagatga ggtggaacgt aatgactaaa attgtgttaa tcacaggagg
    33961 caataaaggg ttaggctatg caagtgcaga ggcacttaaa gcattgggtt acaaagttta
    34021 tataggttct cggaatgatg taagaggaca acaagcatca caaaaattag gtgttcatta
    34081 tgtacagtta gatgttacaa gtgactactc agtcaaaaat gcttataaca tgattgctga
    34141 aaaagaaggc cgtcttgata tcctcattaa caatgcaggt atatctggtc aattttcagc
    34201 accttccaaa ttgacacctc gtgacgttga agaagtatat caaacgaatg tattcggtat
    34261 cgtacgaatg atgaatacat tcgtccctct cttagaaaaa tctgaacaac ctgttgtcgt
    34321 caacgtatca agtggtttag gttcatttgg aatggttaca aaccccgaaa cagctgaatc
    34381 taaagtgaat tcattagctt attgttcgtc taaatcggca gtaacaatgc taacgctaca
    34441 atacgctaaa ggattaccta acatgcaaat caatgctgct gatccaggcg ctacaaatac
    34501 tgatttagtt ggtgatttta gtaataattc caaacacgtt tctgaaggaa tcaagccaat
    34561 cattcaatta gcaacgattg gtgcagatgg cccaacaggt acatttatta atggcgatgg
    34621 agagatgcct tggtagatca attttatgtt acaaacattg aaattagagg tatataaaaa
    34681 atgcttctgc aatagatgca ataaacatgc aatacaatat ggaggcgaag taaatgaaaa
    34741 gtattacgtt tgaagaacat tatgtcattg aagatattca aaaagaaacg atgaatgcga
    34801 tatcagcaga tcctaaaggt gtaccgatga aagtaatgtt agaaggcctt gaaaaaaaga
    34861 caggttttac aaatgccgac gaattatcac atcatgatga acgtatccaa tttatgaata
    34921 atcaagacgt tcaaattcaa gtcttatctt atggaaatgg ttctccttca aatttggttg
    34981 gtcagaaagc cattgaatta tgtcaaaaag caaatgatca attggcaaac tatattgcac
    35041 aatatcccaa tcgctttgta ggctttgcaa ctttacctat caatgagcct gaagccgcag
    35101 cgcgagaatt cgaacgttgc atcaatgatt taggattcaa aggtgcgctc attatgggac
    35161 gtgcacaaga tggttttctt gatcaagaca aatatgacat tattttcaaa acagctgaaa
    35221 atttagacgt accgatttat ctacatcccg cgccagttaa cagtgacatt tatcaatcat
    35281 actataaagg aaattatcct gaagtaactg cggcaacatt tgcttgtttt ggttatggtt
    35341 ggcacattga tgtcggtatt catgcaatac atctagtatt atctggtatt tttgatcgtt
    35401 atccaaagtt aaatatgatt attggacatt ggggtgagtt tatcccattc ttcttagaac
    35461 gtatggatga agctttattc gctgaacatt tgaaccaccc tgtaagctat tactttaaaa
    35521 ataattttta tatcacaccg agtggcatgt taacgaagcc acagtttgat ttagtcaaga
    35581 aagaagtagg tattgataga attctttatg ctgctgatta tccatatatt gagcctgaaa
    35641 aattaggtgt atttttagat gaactgggtt taacagatga agaaaaagag aaaataagtt
    35701 atacaaatgg tgctaaatta ttaggtttat cagctaacaa ttaacaaatg gaggtcattg
    35761 aatatggaaa ctttagaatt acaaggcgct aaattacgat accaccaagt cggacaagga
    35821 cccgtgctca tctttattcc tggtgcaaac ggtaccggag acatttttct gcctcttgca
    35881 gaacagttaa aagaccattt tactgttgta gccgttgatc gtcgtgatta tggagaaagc
    35941 gagttaactg aaccactccc tgattccgct tcaaaccctg acagtgatta tcgtgtcaaa
    36001 cgcgacgcac aggacattgc cgaacttgca aagtcattaa gtgatgaacc tgtctatata
    36061 ttaggttcaa tcgttgcgat gcatgtgtta aaagattacc ctgaagtcgt taaaaagatt
    36121 gcatttcatg aaccaccaat taatacattt ttaccagaca gtacttattg gaaagataaa
    36181 aatgatgaca ttgtgcatca aattttaaca gaaggcttag aaaaaggcat gaaaacattt
    36241 ggtgaaacat taaatatcgc accaattgat gcaaaaatga tgtctcaacc tgctgatact
    36301 gaagaaggac gtatagaaca gtacaaacgt acgatgttct ggtcagaatt tgaaattaga
    36361 caatatacgc attcagatat tactttggat gatttcacaa aatacagcga taaaatcacc
    36421 ctactaaatg ggacagattc tagaggctcc ttcccacaag atgttaactt ttatataaac
    36481 aaagaaacta gcatacctat tgtagatatt ccaggcggtc acttaggtta tattcaaaaa
    36541 cctgaaggct ttgctgacgt attattaaat atgtggggtt aatctatcgt tctctaataa
    36601 aacagcttgg acagttttca aattatattt gatggctgac caagcttatt tttgtttacg
    36661 tttaaaagtt caaatcaact cgttattata catgaaggca tgttaaccat tctcttccac
    36721 tgaaaacact actgttccaa acattgatag tcttcttttg aaataccctt tccgataagc
    36781 accaaatatt ctggcacttt ttttgaaaat gctacaggtg ttaattcata ttgtccttgt
    36841 gtaaattgaa tgagatacgt atgtgcggtg tcttcaaatg tcataaaccc tttcaaccta
    36901 taaatatgag acggaaggca tgctaaacgt tttataaact ctgctttagt acatttggct
    36961 ggtgattgca caaattgatg attaatcatt tgatgtaacg acttatgttt attgtcagaa
    37021 gttgctgtca tttgtctaac tgatattggc aaagtgacgc tgccatgcat accgacttgt
    37081 atatcggcct ctgggttaat aacttctaaa tctttcaata gtttgctcgt tgtttcaaca
    37141 tctgctgaat ctattttatt aacaaataag acagaacaat atgctaattg ctcataaaat
    37201 aagccttgga tgtctttagg gaatgattta atgtgtttat acatgcttgc gtctattaca
    37261 ccaatcatat gtgtaattgt tgtaaacgga gctaaaatag gcgttaaaca agcatctaag
    37321 acagagactg gttctgcaat cccactacat tcaataaata caatgtctgg ttgctctttt
    37381 aaatataatt gatgtaactg ttctgataca tctgctttca ttgcgcaaca gatacacccc
    37441 tctgtcagtt cacttaaagg tatgtcttca gatactaagg caccatcaac actcattttc
    37501 ccaaattcat tcatgatgac tgctggtttc agcgattcct ttaatgattc agcgagcaaa
    37561 tgatttaaga cagtcgtttt accgccacct aaaaacccac ctataataac tattttcatc
    37621 tgcgactctc ctcttatact tcatatgaat agcatattcc aagatagatc tcctattgac
    37681 tgacatgact ctatattcag ttatagttca tttttttaaa taatcagcat tactatttta
    37741 acaatgatgc ctacaccaat aactaaagac aatgatgtca ccatttgctt tccaattaat
    37801 tttagtgctg actgaccacc taaatgtttc aaaagcatcg tcattgtgac cgagcacact
    37861 gtaaaagttg aactaaaaaa cacaagtagt agtaactgtg ttgctgtcat tccttgtaat
    37921 aaggcgccct gatgcaaatt aaacaagagc atgccgtctt ttcgaatcat tgaaaataaa
    37981 atccctggtg acaattctga cgagatgcct aataacgata atataggtgt aaatatttgt
    38041 gataaaacat tcaaaattgg cgttagtgat aaaatactaa caataagaca gattgtgata
    38101 aaaataggta gcgcttgaac gataaacatt tgtatatttt gccacatttg cagcaacatt
    38161 tgacgtatat ttggcatatg taattgtcta tcataaggta gcggaacgct aagttgttga
    38221 tcattctttt tcaaccatat cctattatgt aagatgccac ctaaaagtac taatattaag
    38281 tacggcataa atagccatga ctttccagct acactaaaaa tagataatgt cgcacctatt
    38341 tgataactac aagaactacc aaagcttatt aaactcatac actgtgtctt cgtgcaggta
    38401 tggcattggt gtgctgcttg tgaaatagct gctgcattac atccaaatcc ttctaatagt
    38461 ggcacgatat cattaccttg taatccgatc cataacattg acggttcaat tgcccatatc
    38521 atccatgatt tgagtcctgt ttgatcaatt attgcagttg ataaactaat taatattaca
    38581 accggcaatg cccatacgag cgaatatgtt ccaagtgata gcacaccata atcaccaaat
    38641 aatatatgct gtaatatatc gtgattgagc aatgttgttt gagttaaata ttctatccat
    38701 gctgatacat actgattttg aaaccaatct gaaaattgat aagcgatacc gattggtaat
    38761 gcaaacatta aaaatatcaa caaccaggct aaaaatgtcg cacttatttt aggccttaaa
    38821 tgtcgtcgct catgttgaaa tagtgtcaat tgttctatca catgttcgct cgctataggc
    38881 gaagaaacta cactttctgc caatgaaata tctaacttct gttttatgat taataatctt
    38941 aatttcgctt tttctgttgc ggtaagttta tccgcatgac taataactaa ataatgaggt
    39001 tcattaggta tgggcgtata atgcccagtt ctcatatcat atttcacttc taacatgtgc
    39061 atgacacttc acaacatgaa taatcatcta aatacatttg atttttttga taattttcaa
    39121 tgacatcttg tttagctggt aagccttccc gctgtgttaa aagatgggca agtactgcaa
    39181 atcgcgctct gaatttatag atatagcata atttggcatt atcattttca actgttgcac
    39241 caatcataaa aatattcgga taacgtgtcg attcatcatg tgttgttaat ttaatatctt
    39301 gatttgttgt cacaaataat tgttgaacga ttggattttt tgttgcatca aagccagttg
    39361 ctagtattgg ttcatgaggc gtatgcacac tttgtccgct atcaaaactg atatgatact
    39421 gtccattgtt aaaatcaata tctttaactg tataatgtac attcatttcg atgcgagcac
    39481 cttgcttaat gacattacct agtcgctgac gtgtataagg tgacaatcta acactaggat
    39541 cagcatccgg atcatttaaa ccggttgtgc tagtataaag tgcgatgtca gagccatttt
    39601 ttgcaagttg atatgcagca tcaaagccac tttcattacc tccgataacc acatattgcc
    39661 ccttattaaa gttatcaaag tcttcaattt cactataatg aataccatat ttaaatggct
    39721 ttttagggaa attataatca cctgttgcga caaagatata atccgcgtga tatatctctg
    39781 ttgtcgttgc aatcgtataa tatgcatcat ctacagatat atttgtgaca actgtatttt
    39841 caaagatatt cagctcgtaa tggttggcaa ccacttgtaa atattcagca tatgtttctc
    39901 cggaaatatg ttcttcatta aatgtaaatg ctggtgaagt atccatggaa attgcattca
    39961 tatcaggcat gccaaatcca ttagacgtaa atgatggcgt gatcgtacga gtcgatttcg
    40021 gccaatgttt aaatgaatgt cctactgttc ctttttctaa aataatgaca tctgttatac
    40081 cgaaatcttt taaggtaatg gccataccta tacctgcagc accggcaccg ataatagcca
    40141 ctttatgatg ttgcatatta acactccctc aatgatctat ttcaagttat tgtttttgac
    40201 tataaatcgt aataattacg atttataaag tatagaccta tccttgagaa atttcaatat
    40261 taaaagaaaa acttccgtta ttgtagagaa ttcatctaca tttcggaagt tggtatgtgt
    40321 cttttttatt aaatttaaag atgctacggt tagttcaaat gattatgaat gacatttcat
    40381 ggtatcatcc agcctcagta cttggagata acattttggc ggtgagacag aaatcatatt
    40441 tttacaaaat ttattggggt gtaacttctc cacgttgtat ttcactatcc aaattttgtt
    40501 tcatattttc aaacaactct tgaaatctaa cctcatcttg atcagaataa cctttaaaaa
    40561 taactgaatc tatattttcc tcgttaaaat aataaatttc atcttctaca acaccaatcg
    40621 gatatttaca tgcagaatag tcaaacatat acttttgatt ttcaatttct acaattggtc
    40681 ctctattaat aatcattaac ttttgcgaac cttcttttaa ataaataatg cttcctattg
    40741 tttccattta ttttcctcct ttgtcaccgt ctcaatttca acattccttc gcatttccaa
    40801 gtcaatcatc tcttcttctg ctttacgaat gccatgttga atttgctgtt gctgctcgta
    40861 taacgcttcc gttgaacgtc gtttcgcaaa atgaaattca tcgcggaact gttccatacg
    40921 actagccata ttgctactat aataacgctc acctaaatgt tgatataatt gatctatcga
    40981 atgattaagc gcatcacttt catctatcaa tgctttaacc tctcgctttt catcatataa
    41041 gtcatccatt tgcttatgtt tcgctgtgat tattttattg atttcatcca gtttattcga
    41101 catcatttca ccacgtttta atttttacca atttcattac caatcattac gtcagtttgg
    41161 tcaaaagcct caccaacttt ttgtatatta tcgctcgcag ttcgtattgc ttcagcaaca
    41221 gattgattct tactcatcaa atcatctatc acactatgag cattattatt tccacttact
    41281 gttgtacttt catctttatc cggtttcgac acattattac atgtttcaac agcgctttta
    41341 atagatgatg atgcactatt ggtcttaaaa atatcacttt gaactgtact catcatttac
    41401 acctctctct ccattttaat tattttaaaa ttattagaaa ctgtatcaat atatgatatt
    41461 caaacattca attatactta aatattgttt tatgattata ttcaaaatta ttgtatcaat
    41521 aaatatgtat cattcctaga attttattga acaaaaagtt ataaaataac ttttattgca
    41581 actcaacctt gttttacatt acagaaatta acaatagcga ttaaaatttt attttccaac
    41641 aataaaaaag cacaaatatt tttattgagg cagctgtatc gcttctcatt gtaaaaatat
    41701 ctgtgcatat tattatctca atctaacgta ataactaact tgttgtatct tgtaaaatct
    41761 tattcataga atgcgcaaca ctatcacggt acatttcacc aaatttcaat aaatggacca
    41821 tcaataaata taaacgataa aattcaagtc tataggatgc accttttgcg agtggataat
    41881 gtttattata cgcatcataa aattcgctcg taaaaccacc aaataccgtt gtaataccga
    41941 tatcgaattc tctgtcacca tataatggtg ctggatcaaa taatgcagga cgtccatctt
    42001 ttaaaaacat ataattacca ccccataggt caccatgtaa taaagacggt ttactttgat
    42061 gcttttctaa ttccgccaca atttgacgtc gcactttgtc atatactttg atatcgttgg
    42121 catcccatag ccctctattt agcaactcat ctttcaaatg gtccaagcgc ttgtcgacaa
    42181 aaatggtaca ccaatcgtct tgccaatgat tatcaaaaga aatatcgcca ccttcataag
    42241 gtaatgagaa gccaaatttg ccttcttctt gttgctgact gtgtaattga gctacgagtt
    42301 gccctaattg gcgttgactc cctgaagccc cttcttctaa atacgtcatc actaaatacg
    42361 catcaccgtt aacctcgcca cttgcaatta ctctaggtgc cgtgatacct gcacgttcaa
    42421 attcatttaa acctgcaatt tctgcagcat aaaatgattc tttacgtcca cgttggacaa
    42481 gtaagaaaaa tgtatccgta tctgtttcga ctcgatatgc ttcgtttaca tcaccaccac
    42541 tcactggtga aatctcttta atatctttta aaggtaaatg ctctaaccat tgttcattca
    42601 tacaaacacc ttccaatcta cttactttat actataatat tgcctaatta ggatgattta
    42661 atcttaaaaa cgttattcaa acttgcgtca attttcaaaa ctttttagtt ttaactagga
    42721 aattatgtag ggaaatgcta gtctattaaa agaatatatt tcacatatat taagttgagg
    42781 atagtaagcc tcaaatataa tgaaatagga gcatactcat gtacaaatta attaaacctt
    42841 tcttattcaa aatcgaaccc gaaaaagcac acggactaac tatcgatgca ttaaaaacgt
    42901 tacaaaagtt tccggtttta ttcccagtcg tcgataaact atttacttat aagaatccaa
    42961 cgttatcaca aacgatacaa ggtaatacgt acgacaatcc aattggctta gcagctggtt
    43021 tcgacaaatc ttgcgaagta ccaaaagcat tggaacacct tggattcggt gctttagaat
    43081 taggtggtat cacacctaaa cctcaaccgg gtaaccctca accacgcatg tttagattat
    43141 tagaagatga tgccttgata aatcgaatgg gcttcaataa tattggtatg aacaaagcac
    43201 tcagtcattt gcgtaaaaat gcttatcaag tacctgttgg tatcaatgtt ggtgtgaata
    43261 aaatgacacc ttatgaagcg cgttatcaag attatataaa agtcattgat acgtttaaac
    43321 acgacgtttc atttttcaca gtcaatatca gttctccaaa tactgaaaac cttcaaaatt
    43381 tccatgataa agatgaattt tcaatgttat gtcaagcttt gacaacattt aaaaaacaac
    43441 atgatgtaac agtgccaatt tatttaaaac taacgtctga tatggatttc gatggcttaa
    43501 aagcactatt accagcgatt actgagacat ttgacggtat catcttagca aacacaacgc
    43561 gacaacgaga tggtttaact tctgctaata aagtcgaaga aggcggtttg agtggtcgtc
    43621 cattatttga acgtaattta aaattgatta agtatgctta tcagcaaaca aatggtgaat
    43681 ttttaattat aggtacaggc ggcgtattca gtactgaaga tgcaatcaaa atgatgcgtc
    43741 acggtgcgtc acttattcaa atttattcat cacttgttat tgaaggccca ggtttaacta
    43801 agaaaatgaa caaaggcatc gcacgttact taaaagatca tcattttgac aatgtcagtg
    43861 atattatagg actagatgcc taatccaaca tagtcaaata atactttaaa acttgctgaa
    43921 tcgtcttatt aaaattatga tataactcaa gaagatatat ttatcaccat ttttcaaaat
    43981 aatggcttaa atatcacata tcattaaata tagcgtttta taacggaggc gtttgttatg
    44041 actaaagaaa cagataataa aaaaacagta gcgacttctg acaacaaaga cacacagcaa
    44101 caaaatcaac ctgaagaaga tgaacgtcaa ggtttgaatg gttatcgtaa aacagatctt
    44161 gatttagaaa tcgagcaaga gctacgtgaa atgatgaaaa caggagaaaa tgaaacgaaa
    44221 aatgactata aaaagtttaa agtgttttca cttatttcaa cacttgtcat tgtcatttta
    44281 gcaattataa gatttgttca taaaatgatg taattagagt gagacattgt tttatgtctc
    44341 aggatccagt tattcattat atctacaata tttacgatta tataaataac ccgagatttt
    44401 agtatgattc atttcactaa aatctcgggt ttctatttga taatttttaa tgggatatgg
    44461 catgtatacg ttcttgcctt ttatctcatt tccaatgatt aatctggata ttgttctaaa
    44521 aatgctttcg cttctttatt aactgtttta aaatcaatac cttgttgcat cgctgcaaag
    44581 acacatccac aataacactg cctaaagata ttatagtcat tacacatttc tatggaacgc
    44641 tcataacctt tacttttctt aaaatcactt ggcaaatagt tcacatcgta tattttttgg
    44701 acatccatac caagttcatt gattaattgt gcgttctttt taggtgataa tgtgattgca
    44761 ctgccaaaat aatcatagcc atgttctaca gctgctttcg ctacaatatc caaacgcatt
    44821 tcaaaacaag cggtacaacg taagccgcct tctttttcat cagctaattc tttatccttc
    44881 accattttca caaacttatg cggttcataa ggtgcttcaa tatacttcac attcgcacca
    44941 gtcttgcgat taaaatcttc cacaaattgt tcttgcactt tagcacgtcg taagtactca
    45001 tttttcggat gaatatttga attcgcgaaa taaattgcaa tgtctgcata ttgtgttaaa
    45061 aactctaatg tatatgtact acaaggtgca caacaactat gcaataagat tttaggtctg
    45121 attgcttctc tttcccactg cccgattaat ttcttcaaca ccttgtcata attaattttt
    45181 tgatttttca ttttactgat aataggttca gcattaatca tgatttaacc tcccacattt
    45241 aatcattaac ttctattata tatgattcat attaaatgtc agtcaaaaaa gttagaaatt
    45301 cattttaatg catttatatt tcgaaaatcc cttatgtatc taaaagcatt ttttaaactt
    45361 gaattttaaa acactaaaca acacatacgt ctctgtgtca ttttcatttt ttgtatgtca
    45421 tatatatgtt tacttcattt aaatcaattt catcttataa tttatcgtgt attttacaaa
    45481 agattgactt caattcatcg taaaagttat acttttgcca ttttttaatg taacatggtg
    45541 ttagtaataa aaataataca ttgaggtgtt ttatatgaca gcattattcc cttatatcgc
    45601 ttttgaaaat tcaaaagaag ccattgcata ttacgaagaa gtatttggtg caactgacgt
    45661 taaacgttta gaagttggcg aagaacaagc gtcacatttt ggcatgacta aggaagaagc
    45721 gcaagaagca actatgcatg ctgagtttga agtgcttggc gtaaaagtgc tatgttctga
    45781 ttcttttggt cgcgctgaca aaattaataa tggcatatca ttattaattg attatgatgt
    45841 taacaataag gaagatgctg ataaagttga agcattctat gagcaaatta aagatcattc
    45901 ttcaattgaa atagaattac cgtttgctga ccaattctgg ggtggcaaaa tgggcgtctt
    45961 taccgataaa tacggtgttc gttggatgtt acatggtcaa gactatactg caatccaaca
    46021 ataatgttat tgataaagat atcatttaat aagcttatgt tatttagcaa ggcaagccac
    46081 tctcgattca tggcttgcct tattttattg tatattttta attatcgttg ctggcccttg
    46141 attgacaatc gtattaaaat gccttattaa gtcaactttg tctatacggt ttggatcttc
    46201 tacccaatgt cttataaaag acaatcccgc acctgaaaca taactcatga aataagaaaa
    46261 tggtatacca ttaatttgat cattttcatt tttataacgt tgtaaatgac acataattaa
    46321 atcataaagt ttttcgtgga tatttgaatc tttacccata cgaaacatca attgataaaa
    46381 tgcgatgtct ttttctatca tttctattaa aacggtcata atttgatgta tgttatccgt
    46441 ggataactta actgctccat ttaacttctc atcatgaatg aagtctctta tttcctccaa
    46501 ctgctggtcc tctaattttt caagcaaatc atacttatca taataatgcg tataaaatgt
    46561 actacggtta acatcagcta aatctgcaat ttgttgcaca gtaatctctt ctaattggtg
    46621 ttgatgtaaa agttcaataa atgcatttct cattgcaact tgtgattttc taatacgtcg
    46681 atctatagtc atttgtatca agtcctcccc aatgattata aacaattata aatgttattt
    46741 tcattatcct acaaatctcc aacattgatg attggcacac aatgtttacc tgtttaatat
    46801 aggtgataca aacaaacaga aaaaggtgat aacaatgaac caacatttac taggaaatcc
    46861 aaaattaact gtaactcatg tcaatgaagt gaaagccggt attaaccaca tcgttgtcga
    46921 cagtgttcaa tatggaaatc aagaaatgat tatggaaaaa gatgtcactg tggaaatgcg
    46981 cgatggcgaa aaattatata ttaatatttt cagaccaaat aaagatggca aattccctgt
    47041 agttatgtct gcagatactt atggtaaaga taataagcct aaaatcacca atatgggtgc
    47101 cctttggcca acattaggcg ccattccgac atctagtttt acacctgaag aatcaccaga
    47161 cccaggattt tgggtgccaa atgattatgt tgtagttaaa gttgcattac gcggtagtga
    47221 caaatcaaaa ggcgtcttat ctccatggtc aaaaagagaa gcggaagatt attacgaagt
    47281 gattgaatgg gcagcaaatc agtcatggag taatggaaat atcgggacaa atggtgtttc
    47341 ttatcttgcg gtgactcaat ggtgggtcgc atcattaaat ccaccacatt taaaagcaat
    47401 gattccttgg gaaggcttaa atgatatgta tagagaagta gcctttcatg gaggtatacc
    47461 ggatactggc ttttatcgtt tctggactca aggtattttt gcgagatgga cagataatcc
    47521 aaatatcgaa gacttgattc aagcacaaca agaacaccct ttgttcgatg atttttggaa
    47581 acagcatcaa gtgcaattat cacaaattaa aacacctcta ctaacatgtg ctagttggtc
    47641 tacacaaggt ttgcacaacc gtggctcttt tgaaggattt aaacaagctg tatcagaaga
    47701 aaaatggcta tatgtgcatg gacgtaaaga gtgggaaagt tactacgcta gagaaaatct
    47761 cgaacgccaa aaatcattct ttgattttta ccttaaagaa gaaaataacg attggaaaga
    47821 tacgcctcat gtcatttatg aagttagaga tcaattttat aaaggcgaat tcaaatcagc
    47881 gtcagcgttc cctttaccta acacagaata tacaccattg tatttgaatg ctgaaaatca
    47941 cacattgaat catgcaaaga ttagtagcgc gtatgtcgca caatatgact ctgaagataa
    48001 acgacaagat gtaagtttta aatatacgtt tgacaaagat actgagttag ttggaaacat
    48061 gaacttaaaa ctatgggtaa gcactaaaga ctcagacgat atggatttat ttgcaggtat
    48121 taaaaagtta gatcgtcgtg gtaatgaagt taacttccct gattttaatc atattgaaaa
    48181 tggtcaagta gctaccggtt ggttacgcgt atcacatcgt gaattagatc aagaaaaatc
    48241 cacaattgcg caaccttggc ataaacatga aacagaatta aagttgtcac aagatgagat
    48301 tgtacctgtt gaaatcgaat tgttaccttc aggcacgcta tttaaacaag gcgaaacatt
    48361 ggaagttgtt gtaaaaggta gtgaaattgt aattggtaat agtacccctg gcatgaaaac
    48421 gcgttatgaa catgaagaaa ccgtaaataa aggcatgcac atgatttata ctggtggtaa
    48481 atatgattca caattaatca ttcctatcgt taattgatat gcagcaatta cggtcgcttt
    48541 tgattaaaag tgacatagtg atagaagaga aggccacacg cttggaatct taaaccaagg
    48601 tgtggccctt tttattattg atggctattt aattttataa atcttgtcac taaacttact
    48661 acaagcaata ttaaaccaat aaataaagcc attattgctg tattactttc gttgtttact
    48721 cctgtttttg gcaattcttt caattgttgc ttatgtactg acttgctctt gctatccaaa
    48781 tgaatacttt gttgtttagc catttgttta ttactttttg tgtgaatatc atttgcttta
    48841 atacttggat ttacagcact attattcgat gttccatttg cgtttttcaa ctgattaata
    48901 acatttggat ctttcgtgcc atctattaaa ttatctggat gttttagtaa atgtctgcag
    48961 tcggatacta ttttagaatc ttttgccttt accgcattat tcggcacctc attatcatct
    49021 ttcaactgct tgataacatc tggatctttc gtgccatcta ttaaattatc aagttttttc
    49081 acacgtgcta tgtcactaac tggcccagga taatttgcat ccggttgtct aactggtatt
    49141 aaattgctgt tttcattcac accttcaggc ttgttatttt cggcgttcgc attatccgta
    49201 aaaatcaaca ctgataaaat tgcaacgata atacctttac ttctattacg ctaacccaat
    49261 tggcataaaa ataaaaaccc ctaatggata actacattgt tatctactag aggtttctaa
    49321 cttatactat tttataacac tatcgtattt tctttttcat gaatcatttc aataatgaca
    49381 ttgtcttcat tcattactgc tactttaggt gcatggtttt gaatttcttc ttcattcaac
    49441 tgtgcataag tcatgattat gactacatcg cctacttcaa caagtcttga tgctgcaccg
    49501 tttaaacaaa ttttaccact acctctttca ccagctatta cgtatgtttc aaaacgtgca
    49561 ccattattat tattcacgat ggctactttt tcatttggca agatgtctac cgcttccaat
    49621 atatctgaat caatcgtaat gctacctaca taatttaaat ttgactcagt cactcttgct
    49681 ctatgaattt tagcattcat cattgttctt atcactttat tcagctccaa ttattatatt
    49741 atctattaaa cgcgcttttg aaaatttaac agctaacgag ataaatatgc gtccagttat
    49801 ttcgtgttgt tctactaatt gaggataact ataaacagca acttcttcaa tgcgtccact
    49861 tatatgtgat tcaagatatt cagtaaccct gtctataatt actttacttt gacgttcacc
    49921 gtcttgatac aacgcttgtg ctaatagcaa acttttactt aaatgtaccg cttcttgtcg
    49981 ttcttgctct gttaaataaa catttcttga acttttcgcc aaaccatctg cttctcgaac
    50041 gatatcaata ccaataattt caacggcatg attgaagtct tttaccattt gctcgacaat
    50101 agccaattgc tgggcatctt ttttaccaaa ataagcataa tccggcataa caatattaaa
    50161 tagcttatta actactgtta ccaccccatc aaaatgccct ggacgcttcg ctccttctaa
    50221 cacatcagct aatggaccta ctttgacatc aatacctaat tcacctggat acatatcttc
    50281 tactgcagga tgaaaaacaa tgtccgcccc tacttccgat actaattcta aatctttatc
    50341 aatttgtctc ggataagcat cgaaatcttc gtttggacca aattgtaatg gattaacaaa
    50401 tacactcaca actgtaatat catttgtact aactgattcg cgtaccatcg ttaaatgtcc
    50461 atcatgtaag gcacccattg ttgggataaa accaatcgtt gttcctgagc gtttggctgc
    50521 tttaacaatg tgttgcatct cttttaccgt agtaatcagc ttagtcattg ttattaacct
    50581 cattcataat tttcttttta tacgtatatt cttctgatgg aaatgcacca gatttaactt
    50641 cttgatcgta ttgttttaaa ccatccacac caacactaaa atcagcaaat tgcttcacaa
    50701 atttcgcttt atgttcaaca ccataattta acatatcgtg ataaaccaat acttgaccat
    50761 ctgtaccttt tcctgcacca ataccaatga ctggaattgt taagtgcttg ctaatttctt
    50821 ctgctaaatc atttggaatt gcttcaagta ctaacgcaac tgcaccagct tgttctacat
    50881 ttttcgcatc taaaataagt tgctccgctg cttctttcgt tgcaccttgt aatttatacc
    50941 ccataacgcc aacactttga ggtgttaatc ctaaatgtgc aacaacagga ataccaattg
    51001 ccgttgcttt ttcaataaat ggtgtaatat gcgctccttc tgctttaatt gcatttgcat
    51061 tcgtctcctg ataaagcttt agagcatgat ttaagtcttg tgtcatagag atgcctactg
    51121 caccaatcgg catatcaaca actacaaatg tatttggtgc gcctcttctt actgcacgac
    51181 cgtgatgaat catatctgct aacgtcactt gtacggtact ttcataacct aatacagtca
    51241 taccaagtga atccccaaca agaatcatat caatacccgc tgcttccact tgtttagcac
    51301 ttggaaaatc ataagctgtt accatagaaa ttttagtttg cttttgtttc atatctatta
    51361 attgacttac tgttttcaat gttattcaac ctctttttgc agtattatta gaaatagtat
    51421 aatgaatctt ttatagaaag gaaagaagaa gatgttatca gttgcgatta tcggcccagg
    51481 cgctgtcggt acaactattg cctatgaatt acaacaatca ttgcctcata caacgcttat
    51541 cgggagacac gccaaaacaa taacatatta tactgtacca catgcacctg cacaagatat
    51601 tgttgtgaaa ggttatgaag atgtcacaaa cacatttgat gttattatta ttgcagtcaa
    51661 aacacatcaa cttgatgcag ttattcctca tttaactcat cttgcacatg aggacacgct
    51721 catcatttta gcccaaaatg gttatggtca actcgaacat atttcattta aaaacgtatg
    51781 ccaagcagtt gtctatataa gtggtcaaaa gaaaggcgat gttgttacgc acttcagaga
    51841 ttatcaatta cgtatacaag ataatgcatt aactcgacaa tttagagatt tggtccaaga
    51901 cagtcaaatc gacattgtat tagaagcaaa tattcaacaa gctatttggt ataaattgtt
    51961 agttaacttg ggcattaatt ctatcacagc actcggtaga cagacagttg caatcatgca
    52021 taatcctgaa atacgtacac tgtgtagaca gctattacta gatggttgtc gtgttgcaca
    52081 ggcagagggg ttaaactttt cagaacaaac tgtcgatact attatgacta tatatcaagg
    52141 ttatcccgac gaaatgggaa cgagcatgta ttacgacatt gtgcatcagc aacctcttga
    52201 agtcgaagca attcaaggct ttatttatcg acgtgcacgt gaacacaatc ttgacacccc
    52261 atatcttgat actatttata gctttttacg cgcgtatcaa caaaatatgt aagctcatat
    52321 ataatataaa aagaccttaa cgatgcattt agaaccacct gttccttata tcgctaaggt
    52381 cttttaatta tattagacta ttcagcttct ctaatttctt ctgccacatc tttatagtct
    52441 attttcgctt taacaaacgt ctcgttattt accgggaaat gttgttggaa tgtttcaaag
    52501 ttttgtatct caacgacaac gtcatccact tcaaagtcaa gaacatgtcc accataagct
    52561 ctttcatcat ctgcaaaatg tatatgaaaa ccagcagatc ctacgccatg aaataattct
    52621 ggtgtaaaaa atccaacaat ggcaccacga atattttgtc ttttttcctc aggttgtctg
    52681 cgtgctgaat caatcaaacg tgtatatggc ggttgctgag caggcatcat tcgtacatgc
    52741 atatgtttaa atgtgccata aattttaaca gccgaaaata aattctcact taacatttca
    52801 tttttaattt gtgcgaatac atcatcttgt gataattgtt gcaatgaaaa tgtcttactc
    52861 gctttaaaat tagtaatcga tgcatacggt actttctcat cgccttttaa ttctataaat
    52921 tctttatgct cgttagcatg atatgccttc ccgtctaaaa atattacttc gccatcagac
    52981 cctgttaacg ttgcaatccc taaattccca tgttctaaca attcattaat tgtagctgtg
    53041 ccttctagta agccagccat taacgtacct aatgtaccat gttgatacaa gacattcgtc
    53101 attccaaaac catctcccta tagttattcc tcgttgtcat gctctacttc gtgattaatg
    53161 cagaactact ttcctttata ctcaatgacg cctcaacatt tttattcatg tcactaaatt
    53221 ttcacgagtc ataatacttt attcaattat tattttacgc aaaagtattg cctctatcaa
    53281 tgcttgctaa aaaaatggac ctatcagtgc cacgtgttac tgcaacacta atagatcctc
    53341 ttaccattaa tatatttcta attaatactt aaaataggct tagtcttcta ataaatattt
    53401 aattgaatca aatgtatctt ctaatgtttt aacagattta tcgaacaacg cttgttcttc
    53461 ggcacttaat ggcatttcat aaattttaac tgcgccatgt tggttaacta atgttggtac
    53521 acctaggtaa acgcctttgt gaccaccata ttgaccatct aattgtatag aaacatttaa
    53581 gacattattt tcattattta aaatggcttt tgaaatgcgc atcaatgcta atgcaatacc
    53641 atagtatgtt gaccctttag cttggataat ttcataagca gcgtcacgtg tatttacata
    53701 aatttcttcc gctttagctt cgctaccagt ttgttctttt aatgtgtcat atactgaaat
    53761 acctgctaca tttgcttgtg accaaactgc aagttcagta tcaccatgct caccaataat
    53821 actagcgtca acacttgaag gtgcaacacc aagttcttgg ctaattaaat attgtaaacg
    53881 tgcactgtcc aatacagtac ctgaaccgat aacacgctct gctggtaatc cagtatattc
    53941 ttttacaaat cttgttaaaa tgtctacagg gtttgccgcg attaagaaat atccatcaaa
    54001 gccactatcc ataacactct taacgatgct cttcataatc ttagtatttt tttcaactaa
    54061 ttgtaaacgt gtttcacctg gcttttgagg tgcaccagct gtaataacaa ctaaatctgc
    54121 atctttacag tcttcgtatt cacctgcttt cacatcaact ggtgaaggac tgtggactgt
    54181 accatggttt aaatcttgaa catctgcttt tactttgtct tttgcaatgt caataattac
    54241 aaattcatca gcaacacctt gcgtaaccat tgcaaaggca tagcttgatc ctacagatcc
    54301 atctccgatt aatacaacct ttttaccaaa tgttttcata attatgtctc ctaacctaat
    54361 tgaatttatt ttacacgtga ctacacgtaa aaatatctat actgaaatta aagattaaca
    54421 attttatttt attataatat tatgctacca aattacaaac ataaaactga gatgtaagcg
    54481 gagtcaaatt acactctatc gccaataaat attgatatct caagctttag cacgtaattt
    54541 gaaatttgtg acaattttta gcattctaaa attgcgtaac gacgctattt aaaggcttta
    54601 acaaactatt gagctttcta tttttttcat tttaaaaagg atggctattc gacttaaatg
    54661 acttactata taacctccta ttttccgcct taccctcatc atatttgatg catcatttta
    54721 tagtcaaaga attacatttc atacaaacta gccaatcaga cataaacatg aaattgattt
    54781 taaaaatata atgtgcaaaa ttgttagaaa attagcaaaa aatagtactt attcatttaa
    54841 tatctgttat aatcagtttt atcatttaaa aaatctatgg atgttatcaa gacaagtcat
    54901 taatacgtaa cataaagttg tgatttttca gaatttttag acaaaatatt gattatttgt
    54961 gctatgatat aacatattaa aaaacaccgt atagaattgt ttatgaatgt ttgggaggaa
    55021 agtttatggg aagttttttc aataaaatag cacgaaaaga ggatccggct atctatcaaa
    55081 ataaagatgg tcatttaaag cgtacacttc gggtgcgtga tttcttagct ttaggtgtag
    55141 gaacaattgt atcgacatct atctttacac tacctggcat tgttgctgca gaacatgcag
    55201 gaccggccgt tgcgttatca ttcttactcg ctgctattgt tgctggttta gttgcattta
    55261 cttatgcaga aatggctgcc gctatgccat ttgcaggttc agcctattct tgggtcaatg
    55321 tattatttgg tgaatttttt ggatgggttg ccggttgggc tctattagct gaatatttta
    55381 tcgccgtagc ctttgttgca tcaggattct cagcgaattt acgcggactt gtgaaaccaa
    55441 ttggcatcga attaccagca gcattatcaa atccatttgg tacgaatggc ggttttatcg
    55501 atattattgc tgctatcgtt attttattaa ctgcattatt actatcacgt ggtatgtcgg
    55561 aagcagcacg tatggaaaat attttagtta ttttaaaagt attagctatt attttatttg
    55621 tcatcgtagg tttaacagca ataaatgtta gtaactatgt gccatttatt ccagaacaca
    55681 aagtaactgc tacaggtgac tttggtggtt ggcaaggcat atatgctggt gtttcaatga
    55741 ttttcttagc gtatatcggt ttcgattcta tcgcagcaaa ctcagcagaa gcacttgacc
    55801 ctcaaaagac aatgcctaga ggtattcttg gttctttaag tgttgctatc gtattattta
    55861 ttgctgtagc acttgtgtta gttggtatgt tccattactc acaatacgca aacaatgctg
    55921 aacctgttgg ttgggcttta cgtcaaagtg gtcatggtgt tgtagcagct attgttcaag
    55981 ctatctctgt tatcggtatg tttacagcat taattggtat gatgttagca ggctcacgtt
    56041 tactttattc atttggacgt gacggcttat taccttcatg gttaagccat ttaaacgaca
    56101 aacatttacc taatcgcgca ctagttatac ttactattat tggtgtttta attggttcta
    56161 tgttcccatt cgcattttta gcacaattaa tttcagcagg tacacttgtt gcatttatgt
    56221 tcgtttcatt agcaatgtat cgtttgagaa agcgtgaagg taaagatcta ccaattcctg
    56281 catttaaatt acctttatat cctgtgttac cagcagttac atttgtttta gtattgctag
    56341 tattctgggg attaggattc gaagcgaaat tatacacttt aatttggttt attgttggta
    56401 tcattctata tttaagttat ggacttagac attctaagaa aaatgacgta gctgaatacc
    56461 acccaccaaa ataaaaatca aaaaaatcat ttcccatctg attaacatcc agatgtggaa
    56521 atgatttttt attttatatt aagataatcg ctattaccaa ccttgtccag atatgtcata
    56581 ttgatctaag ttacctgctt ccaaagcagt cagtgcatct tctatagtgt ttaacgccgt
    56641 gtctaattgc tcatatgtta ttaccaatgg cggttggaat cgcaacacat ttcctgctac
    56701 agctataata actacgccat gctcaaagca gtaattacaa attttaagtg ccgcactgcc
    56761 atcacgtgtt ttgagttttt tgtcggaaac aatatcaata ccaatgctca aacctttacc
    56821 tctaacatcg cctacactat tgtatttaga tacccattga tccattcgtt tcctaacata
    56881 ttcccctttt tccgcactag cctgaagaag cgactgatct tcaatcattt gaattgtggc
    56941 taatgcagct tcacaactaa ctggattagc acctgttgta aataaatgtg ctggcgcttc
    57001 taaacaattc ataatctctt tgcgtccaac aattgctgac ataggcatac cacctgctaa
    57061 agattttcca aaagtgatta aatcaggcgt aaaattaaaa tgcgagactg aactccatgt
    57121 acctgttcgc ccaaaacctt gttgaatatc atcgacagcg attaaaatac catgttcacg
    57181 acaaatcttt tctaacgctt caaaataccc tggaactggt tctaaaagtc caccatcgcc
    57241 ttgtatcgtt tcaataacaa tacatgctac ttcgtcagca ggtacatatt tcgcaaacat
    57301 ttcttttaag ggtgctaaat attcttctac tgaattagct tgtggctgct cgtacatacc
    57361 acgatattta tctggaaacg gaatatgata aaaaccattc aataacggac cataatgttt
    57421 gcgcatattt aaactaatag ctgacataga caatgagcca aaagttgaac catgatatgc
    57481 attagtgaaa ctaatgatat aaggacgccc tgtatatgct ctggcaaatt taatgatgcc
    57541 atcatttgcg tctgatccgg ttaaatcgaa ggtcactctt ttttcaaaat ctccaggtgt
    57601 aatctcacaa agcttcttag ctaaacgtac caacggttca tgatacatat atgctggtgt
    57661 ataatgaata aatttatcaa cttgtgcctt tatcgcttct gttacttctc taggtgcatg
    57721 acctacgttt tgagaactcg cactcgataa caaatcgata tatgtcttcc cctcaatatc
    57781 aaccaatgtt gctccatatc catgatcaat cactaacgga taatatttaa tacgtcctga
    57841 tttcgcaaaa taatgttcat cctcttgaat taattgatgt gctttactca tatacattcc
    57901 cctcccattg acagtattaa tgtgcaagca aagtggcctc tcttaaatcg attataaaat
    57961 ccgatttaaa atactgctca aataccgtca tgcctctatg aatactatcc aatatactaa
    58021 taaatttcag atttgtaaat atattctgat aattaagaat tttatatcaa tctactttgt
    58081 ttatgcgcat aattttgttc gattagggaa ttgtttacta tattccattt gactctgagg
    58141 tgatacgaat gcgtttaaaa gtattgttcc atttcattgc tgctattttc atatcattca
    58201 tgttgctatg gatgaccatg ttgtttgact taataagcaa tcaatcacat ttaaaagctt
    58261 tattattgaa tttagatttt ctaattcctt ctgacaatac accatttatt ctagaaataa
    58321 tatgtcactt actaattggt agcctcattt attttgtatt tgtgttactg tttcatactt
    58381 ctaaaaggct ttactatcta tgttatatcc ctttattttt cttatttata gcattgtatc
    58441 cctttttagt atttattgcg caacgtccaa tttttcaatt tagtgtgaca gaattaatcg
    58501 gttggattat tacacatatt ttctttatgt cattaatggc gttagtcata cctagaataa
    58561 aataatattt ttaattaaac tgacaattat ttttcacatt ttatacactt ttcaaggttt
    58621 atgacttgag aaatgttctc atttttatca tactggaggt gtattgaaaa gttaaggagt
    58681 ggaaagtatg aataaagagc aattagaaaa aatgaaaaat ggaaaaggct ttattgccgc
    58741 attagaccaa agtggtggta gtacaccaaa agcacttaaa gaatatggtg taaacgaaga
    58801 tcaatatagc aatgaagacg aaatgttcca acttgttcac gatatgcgta cacgtgtggt
    58861 aacttcacct tcattctcac cagataaaat tttgggtgct attcttttcg aacaaacaat
    58921 ggatcgcgaa gtagaaggca aatacactgc agattactta gctgataaag gtgttgttcc
    58981 gttcttaaaa gtagacaaag gtcttgctga agagcaaaat ggtgttcaat taatgaaacc
    59041 aatcgacaac ttagacagtt tattagaccg tgcaaacgaa cgtcacattt ttggtacaaa
    59101 aatgcgttct aacattttag aattaaatga gcaaggtatc aaagacgttg ttgaacaaca
    59161 atttgaagtt gctaaacaaa ttattgctaa aggtttagtt ccaattatcg aaccagaagt
    59221 taatattaat gcaaaagaca aagctgaaat tgaaaaagta ttaaaagctg aacttaaaaa
    59281 aggtttagat aacttaaatg ctgatcaatt agtaatgttg aaattaacaa ttcctactga
    59341 accaaactta tacaaagagt tagctgaaca tcctaatgtt gttcgtgtcg ttgtattatc
    59401 aggtggttac agcagagaaa aagcaaatga attacttaaa gataacgctg aacttatcgc
    59461 aagcttctca cgtgcattag ctagtgattt aagagctgga caatctaaag aagaattcga
    59521 taaagcatta ggtgacgctg tagaatcaat ctacgacgca tctgtaaaca aaaactaatt
    59581 tatacattaa acatattttt tgatataaac tactaaaagg actcatacat gtgctttaaa
    59641 ggcattgtgt atgagtcttt ttgattaaag ttgattttgc catatgtatt atagattacg
    59701 cgaaaaaatc ggacagctga aaaatctata agatttctca gttgcccgat tttaaatata
    59761 gctattagaa tttagttttc gtagtaacct aattctaagt cttttgaagt ttgtttacgg
    59821 atttttctca ttaatttttc gtcttcaatt aatgattcac cgtatgatgg aatcattttc
    59881 ttgattttag gtgcccattc agttttgtat tcagggaagt tacgttctaa tacttctaac
    59941 gcaactgaaa ctgaagttga agcccctggt gattcaccta ataatgcgat tacagtgtgg
    60001 tcttgtgagt taaccacttc tgtaccgaat tggatgaatc ctttaccgtg ttcaggtgta
    60061 tctttgataa cttgtacacg tttaccagca gtgtataatt gccaatcttc attacgtgct
    60121 tctggataga aagtacgtaa gtggttcata caaccttctt ttgtcataat tacttggtca
    60181 aatgagtatt taattaaagg taagttttta actgctgctg ctaataaagt tgtaatgttg
    60241 tatgttttaa cagacttgaa taaatctaag ttagaaccat ttttcaagaa tttaggtcca
    60301 acattagcaa atggtccaaa taacaatgtt ctttggccgt caatgtaacg cgtatctaaa
    60361 tgaggtacag tcattggtgg tgtaccaggt ggctctttac cataaacttt ggcatcgtgt
    60421 tgttcaataa cttgtgggtt tgtacaagct aagaattgac cactgatagg gaatccaccc
    60481 aaatgtttac tttcagggat acctgttttt tgtaataatg gaatcgctcc accgccagca
    60541 ccgatgaata catagtcagt tacttgtttg aatttctcac cagttaggcg atttttaaca
    60601 gtaacttccc attgaccatt tgataattgt tcaaaatcaa caacttcatg gttaaattgc
    60661 actgtagcat ttggatgtgc ttcaatgctt ttagccattt tacgtgttaa ttcaccgaag
    60721 tttacatctg taccttcgtc aattttactt gccgccatga taccagggtt atcttcacgg
    60781 cctttcatca tcaatggaat ccattttttc attacttcga tgtcttcagt atattcgata
    60841 ttatcgaaca tagggaaagc tttcatcgct tcgtaacgat cttttaagaa tttaacattg
    60901 tttttacctc taacataact gatgtgtggt aatggattga taaattctct tgggttctcg
    60961 atgctaccgc ttttcactaa gtgaccccag aattgttttg aaatctcaaa ctcttcgtta
    61021 atcactttcg ctttttcgat gtcgatagaa ccatcaggtt gtaaaactgt gtagttcaac
    61081 tcacataatg ctgcatgacc cgtaccagca ttatttcttt cgtttgaact ttcgattgca
    61141 ggacgatcca agcgttcgta aacgtggata ttccagtctg gctcaatttc ttttaacatt
    61201 gaaccaaatg ttgtgctaag tacaccggct ccaattaaaa cgatgtcttt actattagac
    61261 ttagccattg gtttcacctc tccaaaaatt gtaaatgtgt atcatcaata tgaaagttac
    61321 ataaaactga catatttctt taaaatatca acgccataga taacttcctg ttttaattga
    61381 tacgctgtaa caaaatacta tagttagtgc ttacatgtat atgttaaagc aagcagtggt
    61441 aaatgtaaat tataattatt cattaacttt gcaatatatt aaatctttta ttcatagaag
    61501 ataaatatca aatcgatcat aattatttga caacaaatag ctaacgattg ttttaatcta
    61561 catttggatt atagcatttt aaacctatac tctattttga tacaatataa gtgtaaaatc
    61621 aatcataaaa aggagattca atatctgcat ccaagaaaaa catcacaatt acctttaata
    61681 ttgaaaacta ttttgaatct ttcacttata ggcattgtga ttatcgtttt tacacatgct
    61741 gagtttcaac ttaatgccgg tgatagtaat gtgtttttat taacatggga tattcgaggt
    61801 ttaacatctt atattttctc attcttaata ctggttgcgc ttgtttctaa actaatttta
    61861 atatttataa ctagacgaca aagaacatca taaataaaca agcagtccag tgcgtatcat
    61921 cataccttac gcattggact gctttgctat gtttattgat tgctatcttt ttgttgttgc
    61981 catttttcta tttcagaatc tcgtagttca acacgacgaa ttttacctga gtttgttttt
    62041 ggtaagtcgt caacgaattc aatctctctc ggatatttat atggtgcaac ttcattttta
    62101 acaaattgtt gtagttcttt aactaacgta tcatcacccg cagtatggtc ctgtaaaata
    62161 acgaatgctt taacaatatt tcctcgtata tcatgaggct ttgctactac agcacattct
    62221 ttaacggctg gatgattcgt taacgcatct tcaacttcaa aaggcccaat cgtatagcct
    62281 gaactaataa taatgtcatc tcgacgtcct tcaaaccaga aataaccatc atcatctaca
    62341 tgagctaagt caccagtgat gtagtattta cctgtttgcg ctttcgccgt acgttctggc
    62401 tctttataat accctttaaa aagtgctggc aaatcaagtg gtactgcaat attccctttc
    62461 gtattagcag gtacgctatt cccctcatca tctactacag tgaccgaact acctggaatg
    62521 cctttaccca ttgatccaat cctctgtggt gtatctttta aaaagcctat aagcaaggta
    62581 ctttcagtct ggccatatcc atctcttaca gttaaattaa agtatttctt gaattgttca
    62641 actacttctc gatttagtgg ctcacctgca gaaacggcac tatgtaaatg cgttaagtca
    62701 taatcattta agttctgtaa tttagccatc atacgatatt ctgtcggtgt acaacataaa
    62761 acattaattt gatatttttg aagcaattct aagtatgttt caggactgaa tcttccatta
    62821 aatacaaaag cagttgcgcc tgaacctaat acagataaga aaggactcca tacccacttt
    62881 tgccaacctg gtgctgctgt tgcccaaact aagtcatctt cattaataca taaccaatgt
    62941 tttggtgcca tttgtaaatg tgcaaatccc catccatgac aatgtgtaac ggctttagga
    63001 ttgccagttg taccagatgt atatgacaga atcgccatat catcacgcgt cgtatctgcc
    63061 atttctagtt tgttacttgc gttttctttt tcagcttcaa gtgaaatcca tccatctttt
    63121 tgaccggcaa taacaaattt agttaacgca tcatattctt taattttttc aaattcaact
    63181 gtgaatggct ctagtgcaat aactgcattt atttcaccgt gcgtgatacg gtattgtaaa
    63241 tctttagttc ttagcatttc agaacatgga atgattgcaa cacctaattt taaagcagca
    63301 atatataatt catacgtcgc aatagatcgt ggcatcataa tgagtacttt atcgccttta
    63361 gataaaccgt gcgatgctaa aacattacct actttattag actgttcaat gagctgttgg
    63421 taagtgactg atatatcttc gccttcagta ttatgatata aaattgcctt tttatctggt
    63481 atgtggctat atttttcgat ttccgaaata atgttatatt tttcaggcgc gaatagagct
    63541 gacttttgca taactaactt cctttcatac atccactttt cctgtgatga acattgtaat
    63601 tttataaatg aattatatat atcatacgcc tatctttaca gaattttcaa ttaaataggg
    63661 ttaaatacca aagtcctcga cactacactt tgacatgacg tagcattcaa ggactttcaa
    63721 atgattgagg gttgatatct cgggctagac catatcagct aattcaatac gaatattgta
    63781 tgataattca cgattaatta tttttacatc tgcacctttc gaagtgccac gatgcttgtg
    63841 tgtatgcttg tactcagctg aattttgcca atgataaaat gcttgcctat tttcccacag
    63901 cgtaataatg atatagtgtc taccagctgt tctaggtctt aaaaacctta atgctttaaa
    63961 tccatcaacg ttttttaaat gcttattacg tcgcaaaaat ttttgctcga atgtttcttc
    64021 ttgtccttcg ttaatatata aatgatttaa cacacataat gaatcatttg ataaatcatt
    64081 tatcgcttcc aaaacatcgt aatatgcagt gtcattattt ttttgtattg tgagacaatc
    64141 atccagttct tctattacat aacttctata ttcatcataa atgttcataa taaatgcctt
    64201 catttcattt atatttttgg tcatattact ttatatctat tactaattca ttcccgtatt
    64261 tattaattac aatcatagtt tggcttcttt ttaaaagata agactttgta aaaagtatta
    64321 atatttcatg caaatgggga caggagtcgc ccactatttt tgtgtcttca atttcatgat
    64381 cattatttaa cattagtcat gaaaatagcc gacaacttca tctgtgaaat caccggcctt
    64441 ttattttagc taactttatt tctgatttta cgattttaat tgatcataca gagaaagtga
    64501 tctttttaca atttctaaaa actcatgatc tatattggac atttgatgaa aataagacaa
    64561 aatgttttct gttagcttct cttgttttgg gaatgaatca tcttctttaa tccaaatcgc
    64621 taattcgcct aatggtgttt tatcatcttt aaatgtttgt atatattcgt aaaagctcat
    64681 agtattcctt ctctcaattt acttatataa atcctaccac gaaagctttc aagaaaacac
    64741 aattaaatgt ctatttagtg aactttttaa ggttgtgcac tcttttaatg tctgccaatt
    64801 aggtcaatta atcatcacaa tgtacaatta actctatttt cagttcatat actcacacac
    64861 cgtttttgaa caacacatta acttctcatt tagataaaac gcaaaaaagc ctggcaccaa
    64921 tacaatagat gccagactaa gagcctacta tataaattta tttagcgtat ggttttactt
    64981 cgattgcacc ttcattttca tcatgaacac catgcttata ataatcaata tattgtggct
    65041 ctaaaggctt tctgccacgt ataatgtctg ctgctttttc agctaacatt aaaacaggtg
    65101 cgtgtatatt gccatttgtc gtacgtggca tagcggatgc atcaactaca cgtaaatttt
    65161 ccataccgtg gactttcatt gttaacgggt caactactgc cattggatct gaagcaggac
    65221 ccattttagc actacaagat gggtgtaatg ctgtttcacc atctctacga acccaatcaa
    65281 gaatttcttc gtctgtttgc acttctggtc ctggtgaaat ttctccacca ttgaatggat
    65341 ccattgcttt ttgagataag atatttcttg ctacacgaat tgcttctacc cattcttttt
    65401 tatcttcttc tgttgataaa taattaaagc ggatacttgg tttttcgaat ggatctttag
    65461 atttgatttt caagctacca cgagagtttg aatacattgg tcctacgtga acttgataac
    65521 catgtgcgac cgctgccttt tgaccatcat atcttacagc tattggtaag aaatggaaca
    65581 ttaagttagg ataatcaact tcgttatttg aacgtacaaa tccgccacct tcaaaatggt
    65641 tagatgctgc tgcacctgta cgtgtgaaaa tccattgtaa accaataaat ggcatgcgct
    65701 tgatatctaa gcttggctgt aatgatacag gttccttaca tttatgttga atgtatacct
    65761 ctaagtgatc ttcaaagttt tcacccacac caggtaaatg aacacgtggc tcaatgcctt
    65821 ttgattttag gaactctgaa tcacctatac cagataattg tagtaattgt ggcgtattga
    65881 atgccccacc agacaaaatg acttctttag catcgatggt atgtagtttg ccatttttct
    65941 tatacgtaac accagttgct cttctacctt cataatgaat ttcagttaca aaggcacgtg
    66001 tttcaacggt taagttttta cgcttcatcg ctggatgtaa atatgctctt gaagctgaca
    66061 ttcggcgacc acgatgtact tgactatcga acggtccaaa accttcttgt ctaaatccat
    66121 tcacatcagg tgttttatga tagcctgctt caacacctgc atcaaagaat gactggaata
    66181 aaggattcgt tgctggccct cgttttaact taattggtcc atcatggcct ctaaatttat
    66241 cataaggcgc tgcaccgtat gttttttcta attttttaaa atacggtaaa cagtgtgcaa
    66301 aatcccaagt ttccatacct tctggttctg cccagccttc atagtccatt ggattaccac
    66361 gttggtaaat catgccatta atcgaacttg atccacctaa aactttacct cgggcatgtg
    66421 ctactttacg accgcccata tgtggttctt catctgttga ataaatccaa tcgtaaaatt
    66481 tattgcctga agggaacatt aacgcagcag gcatttggat aaataaatcc caaaaataat
    66541 cactgcgacc cgcttctaat actaagactt ctttatcttt atcttcactc agacgattac
    66601 ctagtacaga acctgcactg cctcctccaa tgatgacata atcatatgat ttgtttttgt
    66661 tactcatcgt tatgacctcc aaagtttgtt gtatttcaaa gtgagtattg catgaaatat
    66721 ttgttagtgg ctgattggtt atcgttgaat tgctaaactt agctaaccaa aacactcgat
    66781 tttcctttta cgattgttac taacagtcat gaaataatct atgattcatt ttgcgtttag
    66841 tccaaattat caatcaacat ttcgccactt atacaaaaca aagtgagtgt cttgtgttct
    66901 tacaatggca ctcaccttat ctaattttta tttgctaaac caattcacta attgtggatt
    66961 tgtatttgtt aaaatgtgtt ttgaaacaag gtactcttct aagccttctt tgcctaattc
    67021 tctaccgata cctgattgtt tgtatccacc ccatggcgct tgtgcaaaat atggatggaa
    67081 atcattaatc cacaccgttc caagtttcaa cttgttagca acgcgttgcg cttttccaat
    67141 atctttagaa aatacagcac ctgctaaacc atatatagaa tcattcgcta attgaatcgc
    67201 ttcttgttca gtttcaaagc cttctacagt aacgacaggt ccgaaaacct cttcttgtac
    67261 aatacgcatt gacgtatcac aatttgtaat gactgttggc tcgaagaata gaccatcttt
    67321 taaatcgtct ctatctggac gtttaccacc aacagcaatt gttgcgcctt ctgctttagc
    67381 tacatccata taagattcga tcttattacg atgttctgtt gaaatcactg gtcccatttc
    67441 agtatcagca tcaaaaccat tacctaattt gatttttttc acgcgatcaa taagtgcttg
    67501 ctcaaatttg tctttaatac tgttttgtac taatattctt gatcctgctg aacaaacttg
    67561 acctgcatgg aaatatccac catttaacgc ttggtctact gccaattcaa aatcagcatc
    67621 atcaaagata atgtttggat ttttaccgcc aagttccaag gcaatattcg taacattatt
    67681 agcagcattt ttcataatat gcttaccagt ctcaatgcca cctgtaaatg atacaaggtc
    67741 aacctcttta tgacctgaca ttacgtcacc aacttcagaa cctgcaccta gaataagatt
    67801 aattgttcct ttagggaaac caacttcttc cattaattca aaaacacgta ttgttgttaa
    67861 tggtgtaatt tcacttggtt tcataactag tgaacaaccc gtagcaagcg ctggcgcaat
    67921 tttccatgat gcttgtaata acggataatt ccaaggtgta atttgtgtaa ctacacctac
    67981 tggttcttta acaattttgc tttctgtatc tggaattggt gaatcaatca tttcgccacc
    68041 gtctttatct gctaatccag caaaatacat aaacacatta tgaatatcat ccatatctgc
    68101 atatgattct tctaacgttt ttccagtatc taatgtttct aatcgtgcta acgcttcgcg
    68161 atgttcttta atcttgtccg cgatggcacg tactttttta cctcttgttt cagcagtttc
    68221 ttgcgaccat tcaccagact caaacgcacg tcttgcagct aagattgcac gttctgcatc
    68281 ctcttttgtc ccttcagaaa ccgtaaatat cacttcttga ttgtaaggat tgataatatc
    68341 tcttgtattt ttattcgcgc tttcaaccca ctcaccatca atatattgac gctgagataa
    68401 atgttttaaa agttccattc aacaacctcc gttaagtttg ttaaattttt atttaacgaa
    68461 ttttacaaac ttatgttagc atagaaatga aaaatgtgtc aatttacaaa acaactcaag
    68521 aattaagttt tgtaagtttg ttagattttt atttaacgaa acatttaact ttaatgcttt
    68581 aaatattttt aatgtctttt tttagaaaag gttttataat gtattagtat ttattttaga
    68641 atgaccaaaa tgatgtacgg aggtgcaatg atgactcgtt ctcagaacaa acagcaacaa
    68701 ttagaacaag caaaagatat tgtaattaat tcaattggac agacaatgga tttgtatggc
    68761 actaatagaa gtgtaggaaa cttatatggc actatggtgt ttgaaggcag tatgacgctt
    68821 gatgaaatgc gtcatcaatt gcaaatgagc aaaccaagta tgagtgctgg cgttaagaaa
    68881 ttgcaggaat ttgatattgt taaacaacaa tttacgagag gaagccgcaa acaacatttt
    68941 atcgccgaga aagacttttt catctttttc cgaaactttt tcactaaaaa atttcagcgt
    69001 gaaattgaca tcaatgtcga agcagtaaaa gatgctcaag ctattatcaa tccattactt
    69061 gaaagcagtg atttaactga agctgaaaca caagaggcac aaaaaattaa agctcagctt
    69121 gatcatacac atgtttatta cgaatggtta gaacagttaa ccgaagcgat tgaaagcggt
    69181 gaaattttca aatatttccc aattccagaa cagccttctg attctgaaaa ttaaaattga
    69241 ctcaaacaac gacaatgatg attatgcatg atgaaaacaa tgcaataggt ccacgaaata
    69301 aacggtaatt tctatctcaa taaatagaaa ttaccgtttt tgttttatat gacaatcaat
    69361 catggaggta ttttctagag ctttcttaga ttcgttacat tgcttcgcca atttatttca
    69421 attagctatt ctcgccctca cgtactctat caataatatt ttgtatttgt tcacgttttt
    69481 catattcatt ttctacaaaa taaactaacg gttcatcttt gatagtaacc caaggtacaa
    69541 gtacatcaaa atctaattct ttaaccatct ctaaactttg aatagatttt tctctatcac
    69601 ttgaacctaa tataactgta cgccatctct tcccttcaaa acatataaaa tctccagtaa
    69661 ataagtaacg gtgatgacca tcatcccata aaaatagtgt cgtccctggt gtatgtccag
    69721 gcgcgggaat aacttctaaa tccttatcca acattacacg attactaaat tgtttttgaa
    69781 cagaaatctt atgtttcagt gcagccacat cattttcatg tataaagtaa ggggcttcaa
    69841 cttgattaga acctcctata gattcatgat catgattcat caatactttt gaaacaccac
    69901 ctaatgatgc aatgtcttgt tgggagtcac ctaagtgtcc ggaatgatag ataacaatat
    69961 tacctgtttc acgttttaaa ataaaagatt taaatagaaa tcgattatca aaaggcagtt
    70021 ccgaagtagg tgtcgcatat aagttttttg tgatggattc taaactgtca tgtaatttgg
    70081 actgtttatt tttaaattga ttagtcattt tatttcatcc ttcgattaat ttgaattgta
    70141 catacgcagt accctttatt taataatcaa tgagagtctt tcaaacttcg atttagaata
    70201 tgcttcatac aataaataag tacttggagc actaaaaaat taaatctttt agtacaacat
    70261 taatgaaaac ctaaaagttc atcctacaat gctactaaaa aaaggggaat ggaacagaaa
    70321 tgatattttc acaaaattca tttcgtcgtc ccagacccgc tttgaattat aaattatcgt
    70381 cttgttcttc ttctgatact tgaacgattc gcaatgaacg acgttcaact tcttttaatt
    70441 tttcagcacg cgtttcaagt ttaattctat cgcgccctaa aatgattaaa aatgatatca
    70501 tcatgaaaat aaaaataaca attaatggca cacttgccag tattgaagca gttttcaata
    70561 cttctaatgc acgttcgcca ccaactagca tcaatgaaaa tggcaataag cacaatgcaa
    70621 atgcccagaa taaacgattg gcacgtaatg gttcgcctac cacttttttc tgagatgctg
    70681 ccgctaaaat atatgaaccc gaatcaaatg ttgttgctaa gaataagaaa gcagatacta
    70741 agaatagtac aatcatcaat gatgggaatg gtaaatgatg caccacttca ataatggttg
    70801 cctctgtacc atgtgtattt aaatattgtg ttacattaaa ctgtccagaa atttgcaaat
    70861 acacagcata gttaccaaaa ataccaaaga ataatacgca tccaagcgtt ccataaataa
    70921 ttgttcctag cacgacttct ttaaggcgtc gaccttttga aattctagcg ataaataaac
    70981 cgataaatgg cgcatatact aaccaccatg accagtagaa tattgtccag tcttgtggga
    71041 aattcgtttc ttttcgacct ttaataccac cgaatggttc taaccatgtt gccatatgga
    71101 aaaaatctct caacatattt ccgaaccctg tcactgtcgt ttccataata aaaacagtcg
    71161 gtccaataat aaatataaag gctaaaagta caaaggatag ccaaacgttg atatcactta
    71221 acttttgaat accttttttc aatcctgtat atgaactaat ggcaaatata accgtgattg
    71281 ttaataaaat ggccgaacgt aaaatcatat ttttaccatc taaaccagtt aatctttcta
    71341 tgcctgcaga aattaatggc acacctaacg ctagtgatgt tgccgcacca cctagcaatc
    71401 caaagataaa taagatatct acaactttac ctacaaattt atctgtttga ccttttaaaa
    71461 tcggacgaca agcttgacta attttataca ccggttgttt tttaacaaat actaaataac
    71521 caattggtaa tgctggtaaa acgtaaatag cccaagcaat tggcccccag tggaacatac
    71581 catattgcgt cgcatattgg agtgcttcat cactcatact tttcgcgcca tttggtggaa
    71641 cttgatagta aaaagcccat tcaataacgc cccagtataa aatatcagag cctatgcctg
    71701 cacaaaacag cattgccgcc catgtaaatg tattaaattc tggtttatca cttgctttac
    71761 caagtgtgac attaccatat ttaccaaatg cgatatacat tacaaagcaa aaaatcgcca
    71821 gccccataaa taaatatatc gaaccaattg aatcagaaat ggcactatta ataccagtga
    71881 tgatatcttc acttgctttt ggaaaagcca tcataggtat aactgcaaaa agaagtacag
    71941 ctactgtccc tataaaggtc gtccagtcca taactttctc ttttttcaat tgtgctcccc
    72001 ctaattatta attttatgaa tcctgtttcg atttatctca aaatgtaata attatattga
    72061 ttcacaaaat tgacaataac taacatttaa taataatgca aatttcatac aatttgaaac
    72121 ttggcaatta ttgaatattt atataatttt tcccgtaata aacaaaacct taatagcgct
    72181 aaaacaatag tgttaagtta tgatttaacg aatttaacaa attttactag gacggcattt
    72241 aagaatattt atacgttatt aacgaatatt tatttattgt aaaacgctac caaaaagtta
    72301 gacttccttc ccactaaaat accacttttc tctttcaact tttttaaaaa acggatatgc
    72361 aacttttagt attggtatca aaatgattgt taggtcatat tctatcaata tatttttcta
    72421 aagaattgct tttattaact ttcaattatg tacctaacct aaaaaaaagc caaggcaacg
    72481 aatgttacct tgacttctaa tacatattca actaactata tattcaatca tacgcgcatg
    72541 cgagagtgat tgttgtacat ctataatgcg ttgatttaaa gaacctttat atggtaaatc
    72601 aggtttgaat aagtgttgta taaatagacc atctactaaa acgtcaatgt atgataataa
    72661 ctctcgacgt tctgcacaat catttgctaa atattcatat aaaaatccag tccataccca
    72721 aattgtcttt ttatttccaa aacgtgctcg aaatgctttg acaagattta atgtaatatc
    72781 caaattacaa aatggttcgc cacctaatag acttagccca gatatataat catgatcgca
    72841 atcatctaat atttctgcta atatttcatc agtgtatttc tcgccatatc tgaacttttg
    72901 tgaggctttg ttataacatc caacacaatt aaatggacat cctgatacat aaacactgca
    72961 tcttactcct tcaccgtcaa caaagctatt tgattctatt ttagcaatat aaccttgtcc
    73021 ttgtttaatg tctaaaagtg tcattcttta ggcgctttca tatgttttac tcgtgcgcaa
    73081 atttctttat gacggccttt aattactgga cgttgaactg gattgcctaa gtaaccacat
    73141 gttcgtttaa cgacatcaac tgttttagga ttatcattgc cacagttcgg gcatttaaat
    73201 cctttttcag ttgcttcaaa atctccatcg taatcacatt cataacaatg atcaatcgga
    73261 atatttgtac ctaagtaacc aactttgtca taagagtagt cccataccgc ttctagtgct
    73321 ttcaaattgt gttgcaattt cggatactca caatagtgaa tgaaaccacc actcgcataa
    73381 taaggataat ctttttcaaa atctaacttt tcaaaaggtg taacatcttt acgtacatca
    73441 taatggaaag agttttgata atatccttta tctgtaatgt ctttaatatc tccaaatctc
    73501 tcttggtcta aacgacaaaa acgatccgtt agcgattcac tcggcgtact gtaaatactg
    73561 aaccaaatgt catataattc tgtccatttc gtttgataac gtttcatttc tttaagaatt
    73621 tcaagcgtaa atgcttttgc ttcttgagat gtttcccagt ctggaccata gaaaacagta
    73681 gctgtttcat acaaccctat atagcccatt gaaatcgttg cacgtttatt tttaaataac
    73741 tcagcaacat catctgtttc ttttaattta tagttaaatg cgccactttt atataaaatc
    73801 ggtgcgttat tcggtacagc atctttcaaa cgatttatac gataaagtaa tgcatcatgt
    73861 aacacatcga tacgttcata aaagatttcc cagaatttcg tcatattacc ggcagattct
    73921 aatgccattc taggtaaatt aagtgtaaca acaccaagat tacaacgacc attattttca
    73981 aaatgacctt ccgcatcttt ccaacttggt aaaaatgaac gacaacccat tggcgctttg
    74041 aaatcaccta atatttctac gagtttgtca taatttaaaa tatctggata catacgtttc
    74101 gttgaacact ttaatgctag ttgtttaatg tcatagttcg gatcttgcgg actaaagttg
    74161 gttccttttt taattgaaaa tacaagtttc gggaaaatcg ctgtcgtgcg gtcttttcct
    74221 aagcctttga tacgagtatt taagatagct tgttgaattt tgcgacttaa atgatctgta
    74281 cctaagccga atcctaatgt tacaaaaggt gtctgtccat tagatgtata taaggtatta
    74341 atttcatatt ctagactttc aatcgcatca ttgatgtctt tagtgacttg ttgatcaaca
    74401 taacgatcaa tttcagattc tttgacaaat tgctttgcga tatttctatg ttgttcttca
    74461 ttatgtcgtg catatgtact aagtaattcg tcaacgcggt caaccgtaca gccaccatat
    74521 tgactgctgg aaacattggc tataatttgt acaagctgcg ctgatgcagt ttgtattgat
    74581 tttggtgaag ttacattcgc gttgcctatt tcaaatccat tatgtagcat atttttagca
    74641 tctattaaac aacagtttgt taacggttgg aatggatgat aatctaagtc gtgaaaatgt
    74701 atgtcccctc tttgatgtgc atctgcaacg tgctttggta ataaataatt taatgcgtaa
    74761 gatttagaaa cgatacctgc tgttaaatct ctcattgttg aaaatgtatc actatctttg
    74821 ttggcatttt cgttaacaat agcaggatct ttagaaatca aacccgttaa tgcttcttcg
    74881 atttgattca ttttacccct tcttcctata tattgtgtct actcattacg ttataccact
    74941 atatatagta ttttcaaatt tttattagtc tgttaactaa aacgtcacaa agttattaac
    75001 atttagtgaa attcatattt ctacacaatt atttcattaa tttttaaaat tgttctcgaa
    75061 tcgttctgtc atttcgacat gtttatgcaa ctattatttc attttgcacc gggactatag
    75121 tttctatttt tcatacttaa atcgtcacaa tgcccatcaa cattgcaatt actaacataa
    75181 cgatggcgaa tccccaaatc caaaagaatg catacttaat atacgtgccc atgtttgcct
    75241 ctgctaaacc aattgccaac cataaggctg gtgaaaacgg actgacaaat gtacctataa
    75301 tattccctat aaccattgaa taagctgttg atacagacgg tacaccaaat tgccctgctg
    75361 tttgttcgac gatcggtaac acagcaaaat aataagcgtc tgtgctagtt agtaaatcta
    75421 atggtacgcc aagtaaacct acaataatat gcaagtatgg tcctacttct gcaggaatca
    75481 ctttgattaa attcgtcgca atcgctttaa gcataccggt ttcatttagt acacctaaaa
    75541 acatacctgc tgcaataatc actgcagcca tcattaatgc attcggcgca tgcgctctta
    75601 atcgctccat ttgttcatcc actgatttaa aattaataac gagcgccaat gagacaccta
    75661 tcatgaatgc aaattcaggt ggcgcaatat ttattaacat cgatagaata acagctaaag
    75721 ttaaagctgt attcacccat tttatccatg attttgttct tgcacgtcct tttacaggaa
    75781 accttacatc ttgatctcgt tcatatactt caactaattt atgtacatct atatcttgtg
    75841 tttgcggtaa ttcatttctc tctattgctt ttttgatacg tttctgttct ttaaatccaa
    75901 gatataccgc aaacaacata ataagaatga atcctattat ttgaatagga attaatccat
    75961 accataattc attgacactt ttggctttta acactgcagc tacacgtgcc attggacctc
    76021 cccaaggtac catgttcata atcgccgcgc ttaatgctaa tagtaaaatc aataaatatt
    76081 tattcatatt taacgcttta tataaaggta ataatgcagg aatagaaagc aaaaatgtta
    76141 ccgcaccggc cccatctaat tgggctattg tgccaattaa agctgtcatt gcacagacaa
    76201 tgacgacatt gcctcgtgtc attattatta agcgtttgac aagcggcttg aataaaccac
    76261 tatcgttcat gatgccaaag aaaataatag caaagataaa cataataaca acgttgatga
    76321 cttgatctaa ccctttagca aaaaatccaa ccaaatctgt cacactatat cctaaaatca
    76381 ttgcacctaa gcaaggtatg attgtcatac caacaactgg atttatcttt ttggcaatga
    76441 gtaaacctac aattgaaata ataataatga gccccattac tgttaaccac atattatcac
    76501 tattcatatt attttctccc tccactttta atacaattta cataatcccc ctctttaact
    76561 taaatattat aattaatata attttttata tttaagttat ctcattttat caaaaaaatc
    76621 ttttttgcgt aatagttaat tcggttatct aatttttatg cagtatctct aatgactatt
    76681 tgaatccaat tgattaattt gaatcttttc ataagtatca tttttaatag caatggttga
    76741 attctcggtt ctattacaca aaaaaactgg ctaaaattag ctattattca tagcaacttc
    76801 aaccagtttt tctaataacc tatatattta ttaagatttt tcaaatcgtc tatttctcta
    76861 tgttagaaat catcttacat atgtttatca tcttcgctta gaaattatta gtatgcatcc
    76921 catcttcatt tacaaacaga catgttatta cttcaagact gcctttagac ggcacacagt
    76981 tattattttt tatccaacca cgctaaaaat tgagcttgtt tagtatcgtt tataaattct
    77041 ttcgtcacta tttgtgataa caattgctgc ttttcgctgt gattcatatc aagtaacttt
    77101 attttctgtc ggcaagtata taaaaagtcg atatacgaac tgtatgatgg tggatataac
    77161 gcctcaagct ctgccataat tgattttgtc aacttaggac tcgcaccatc agttgataca
    77221 ctgatagtta gcttgtcgcg gtgtagtgca cttggaaata caacattgcc atttgatgca
    77281 tctccaacat tattaaaaag ggcatgctca ggtaaggctt gttttaccgc ttcattgaca
    77341 cgtggctcat tggttgctgc aataactaga tacgcgtcta caatatcgct tggttcaaac
    77401 tctttttctt tccatatgac gacaccttta tctaccatat tttgaagttt ttcagtgatt
    77461 tcaggactga taaccattat gtgttcaacg tattgactta atgtttgtgc ccgacgactt
    77521 gcaacgaggc ctccaccaac tatgacgaca tttttatttg ttaaatctat cattaatggc
    77581 atattcatgt tgagcaacat cctttatatc tttttacgtc atttcatctt aaaacattta
    77641 acaccaatcg ctaataaaca tcacgttgat agcgttgttg ctgtttcatt tgacgcaata
    77701 tcgtctccgc ttcttcttga gaaatatgac gttcttttac caatacatct ttaatggctt
    77761 gatggacatc tttcgccata catttttcat caccacaaat atagattgct gcgccttgct
    77821 caatccattc attgaaacgt tcactttctt ccattatacg atgctgtaca tattctttgt
    77881 gttcttggtc tcttgaaaat gctaaatcta cgcgtgttaa gtttccattt tcaagccatt
    77941 cttctatttc ttcttcatat aaaaagtcag aactacggtg ttgatcgcca aagaacaacc
    78001 atgttttccc agtcatacca agttcttcac gttcttgtaa atatgctcta aaaggagcaa
    78061 ttccagtacc tggtccaatc ataataaccg gtatatcttg cttcatcgga aatttgaagt
    78121 tcggattttt cttcaaataa attggtacta tatcgcctgg tttaattcgc tcagcaaaat
    78181 gaaccgagca tacacctttt ctctcacgtc catgtgcttg ataacgaacc gtaccaacgg
    78241 taatatgcac ttcatctggc gttgccataa aactactaga aatcgaatac tctcttggtg
    78301 gtaattttct aaggatttga tacatattct caggttgtaa ttctatagtt ggaaaatctg
    78361 ttattaaatc tataaagtcc cgatttataa catattcacg cgcccatgac tcatcttgaa
    78421 tacgttcaga taattcttca ttgtcaaaat agatatctgc atttttcaat aatggcaatg
    78481 ttaatttagt aaattcaaaa tgtgatgtta gtgcttcaac aataggaact gtatcaccat
    78541 gatcattaat tggcaccgga gattgcggat cccaacctaa catggatatt agtttttcaa
    78601 ccaattcagg gtcgttttgc ggtaatgcta ctatacaatc tcctggttca tatgattcac
    78661 taaaatcatc aagtaaaaat tctatatgtc gtgtttcttt atttgaatcg gtaccattta
    78721 aattgatatt cgctaaaact tctgcttggt atggatttaa tttagaatat ttcttttctt
    78781 tggcagactt aattgattca cttatcaccg attcactttg aataccttct gatgtagtat
    78841 caatcatatt aatgatgtct gccatccact tttctgcgtc ttcttcataa tcaatatcac
    78901 aatctacacg cttacatata cgctcagcgc ctagattttc tagtaaagca tctacatctt
    78961 taccagcttg acagaaaaat tcatatgttt gatcacctaa agctagtact gaatatctca
    79021 catgatttaa attaggtgcg ttatcgtctt ctaaaaattc aaagaaatcc catgcattat
    79081 caggcggttc tccttcacca tgagtagacg taataataaa taaatcttct aactgcgcga
    79141 tgtttgtcgt atcatattca tccattgaca tcaaaacaac ttgatgtccg atatcactta
    79201 aacgttctga aaaaatttca gctaaacgca tggcattacc tgattcagaa ccatatagca
    79261 ctgtaacatg ccgctgatta gcttcgatat gtggctcttt attttgtaac atatacgttg
    79321 ttgaagttgc ttgttcttct gatatttgtg attgttgcca cacttctgtc tctaattgtt
    79381 gttgattact atcacttgtc gtatcgttac tattagctag taaaaaacca ctaagccact
    79441 gttgttggct ctctgttaat gtttgcagca gattgttaat ctctgtgact tgtttttctg
    79501 taaatggact attagatgta tttaatttca aatgttttca cctcaataaa cttgaaatag
    79561 atgattgact cttttttatc gatagtttag tcaatttttt aatatgtatg gtataaatta
    79621 aaacaaactt gagttgtgtc tgatagttat acttgcaaat atttacaact ttcatgttgc
    79681 ttgaatgaca ttatcagtaa ttcagacaaa actttgatta tgtcgtgtca gttattaatg
    79741 catacagtaa tgcatcgttg ttatttttta tttatttact atttattatg taccaaatca
    79801 gcctttcagt aaaataggtc aaattactga ttttctaaat ataaaatgcc tcctaatgac
    79861 atactattag tacatcatta agaggctctt gtgttatttg catactaagc gctcaatttt
    79921 aaatttaaga tgaagattct tgcaataata tttctatatt tgtcgatata tccaatggat
    79981 cttccattgg caagaatcga tttacaacat tgccttgtcg atcgattata aattttgtga
    80041 aattccattt tattggggac ccaaagattc ctggttgttc attctttaaa tgcgtaaata
    80101 acggatgttc atcgttcccg ttcacagata ttttagctag cactggaaat gtaacaccaa
    80161 atttctcacg actaattttc aagatttctt cattagaacc tggttgtcga ttgtcaaaat
    80221 tattattcgg aaaactcaac actacaaacc cacgatcctt atatttttga aacaaagtct
    80281 ctagtttttt caattgttcg ctatatatac attctgttgc agtattaaca actaaaatca
    80341 ctttacctct aaatgcttct aatttataag ttaagccttt ataatcactt acttcgatat
    80401 catacacatt tctattattc ataagacacc cctacacagc cttttttata ttgaatatgt
    80461 tctttttaga atgttctgat aaaataagtg cgcgtttaca ccgcgaacat acattatata
    80521 gcgtgataca tttttcgagc acacgataaa taatgttcga gtttatgttg ttgctcaacc
    80581 tatccgattt accgtctttt ttcacctgtc ttactttatt aaaataatca cattgtatag
    80641 ctgtattagt ccagctacaa tatctttcgt tataacccct cattataatc atccttattt
    80701 tctattttta aaaagacaat tagaccgctc tttaaactat agattaatac ttaagttaaa
    80761 ctcatacata ctgataccat acgttagatc taacaattta aaattcgtta taactatgga
    80821 ttaaagagct gcccaactca tataatcctt aaaaacttca catgtgattg tttattaagc
    80881 cctcctttat cttattaaat atccttataa cccttttaaa attaaactga cacactcata
    80941 cattgttaca cgcgacgata aacgtcatat tattgcatat cattaaaaca agtcactttg
    81001 tatcgtgtgc gaccgaatac atattaaatc atcaataatg attattcaat tattaaaaat
    81061 aaaaacgaac attctatata gagtttctac ttattgtttt ttcatcattg tctacattac
    81121 ttaacattcg tgtttataag taatattgac cgccaatatt tagacacttt ataagtatgc
    81181 cattcatcat ttttaatcat attatttttc ttattgatat ttctattcat atatttctga
    81241 ttgtatattt agatataata atttgttatt ggaaaatata tgaaacaaat ttgactcatc
    81301 tttttcaata tctaaatgac acgatgcatg atttttgaat tttgtaatta acttcctttt
    81361 aaattagact gaattatgac aattttcaaa cattttaatg tgtaaatcat tggcaaaata
    81421 tacgaattgc atttaaatcg tcaatttaca tatatttttg cgtagtaatc aatcgttatc
    81481 attgttattt atcgttacat tatttcgagt atcagtatgt atttcgggct tcgtttgata
    81541 acgacatttc tttgtgacat cgcttcatca gtgtaacaac aaatacaatg atttcgtgat
    81601 gttagttacc cattttatgt gttgcataaa atatgttgtt ataaaacatt ttaaatcatt
    81661 ttatataaac aatctatata tttttgggat ttccaaaata tcacttgtta tattaaaaac
    81721 cgacaagaca ttttatctta tcggttgaaa tttgttattg ttatttgtaa tgtttttagg
    81781 tttcttttta atataatata tttcagtgaa aatacatgat tgattgtgat ttcactgaaa
    81841 catggttaat tgcgttgttg atgaataact ttagcataaa tataggaagt tattttgtac
    81901 atcgccatat atagaaacga aattataatg acagctagta cgtaacttgt taaaaatata
    81961 tgatggttat taatacctat catatttagt aacgtatata caatgttact agaaattaat
    82021 gtgtgaatca gtgctactgt tattggtatt gcgaacaaga aagtcatttg atttcgtgtt
    82081 atctttgcta ttcttccatt atctaaacca agttttttca taatgtcata attgtgctga
    82141 tttgcatagg cttctgaaac ttgtttatag tacatcatta gaaatattcc aatgacaagt
    82201 acaattgata ctacgccacc tacaaatatt agtccacctg tcaaacgatc ccaaaccatt
    82261 agcatttctg atttagaatt tattgacgca tcgtattttt tcgatagctt ttgtatttgt
    82321 gatgttgtta atttcgtctt tccaaataca ttaaagttta tcgatattgt cgaattcttt
    82381 tgttcatgtg cataaaatcc ttttactttt tgtaattgat tgttatcttt aacaattaaa
    82441 accatactat cttgaatcat catcaaattt aacgcatgtg cgtcaagttg tttcacttta
    82501 aagacttcat tattaagacc gaccttagac tgctttttaa aaataggtac attcgtaatc
    82561 atacctatac tttgattgtc taagtgtaat ttgttttggt gatatcgatt ataatcttgt
    82621 gcagtaaaag ctgtaatcat tactgattgt cttgaaaata tcgcgccttc attaccaatg
    82681 aagtctattg gaatatcatc actagcctgt tttaacttgt aataagcacg attcttttct
    82741 aaagaagctc taaataatgt cgtttcatat actttgaagt cttttgcatt aaccgaaccc
    82801 tgtatatcac gtttaatatt ctcaattgtt tgttgacgtt ctatttgtga cttagaattg
    82861 tcgctaaatg acaaatcata atcattcgta atcaatttcg taatggtatg attcatatca
    82921 cgataggttg ttaatgtcat tgtgagcgtt acaatcaaaa atgtacacag caaagtgatt
    82981 gttgcaagac tgactgcatt cattttaaga cgtacacgca tcccaactac cacaaaaaaa
    83041 taccgtggat gatagtaaac ttttgatacc tgctgcaata tactaataat tatttcactt
    83101 atacctacaa aaaatgcata agtaccgata ataactaatc ctatgacaat ccatatttta
    83161 aaaaaggcac caaacgtcgt atcttgttgt aatgcaataa agtagcctaa atatagtgct
    83221 gcgcttccta taacaattaa aacgtaacgc aaccatcgtg atatgacact agaatcggaa
    83281 cgatgttgat aagttatcgg ccgttgaaaa ttaatactga acaagttgaa tataagtagc
    83341 atgcccatta atacagcaat gataatcaaa gtaataaaca tcgctacaaa gtcaaatgga
    83401 taatacctta acgtcgcaac ttcactgccc attattttct gtataaacaa gaaaaatatc
    83461 gcaccaagta agtatccgcc ggcaatacta acgactgaaa ttattataaa ttgtaagata
    83521 gtttccatta ctacaattaa acgcatactt ttcttggtca tgcccaatgt cataaaaatg
    83581 ctaaactctt ttcgtctttg tgacatcata aagtgatttg catagaaaat aaaaataaaa
    83641 gttaaaagcg ccataaaaaa attagctatg ataataaatg gtactaggaa gtcattcttc
    83701 tgttttatgt agctatttaa cccaattgac acaagcgtat attcaatcat gaatagtaca
    83761 cttaccgcta aaatgaatgg aatgattaca agtcgctgcg taataaattg acgtcgcact
    83821 atgtgccata acaatcttaa cttcatatat tgacacttcc tccattcaca agtgctaagc
    83881 tatctgttat tcgttgttga aaagctaatt gactttcttc gccacgatat atttcatgat
    83941 atagacgccc atctttaata aaaattactc gctctgcata agacgcatcg atatttgaat
    84001 gagttaccat taaaattgtc tgttccaatt gatgaatctc ttgaaataac atcatcaatg
    84061 cctttgatgt ttttgaatca agtgcacctg taggttcatc ggctagtaaa atcgtcggtt
    84121 tagtaactaa tgcacgtgca atggcaatgc gttgcttctg accccccgag atttcagaag
    84181 gatacttgtt taagaatccc tctaaatgta attgtactgc caactgatgt accctttgtt
    84241 ctatatcttt tcgtttagca ccagctaaaa ttaatggcat cattatattt tctttattcg
    84301 tcattgttgg taaaagatta aaatcttgaa aaacaaaacc taccatttgt tgacgataca
    84361 gtgctttact tttatttttc atattattta aatgtgcgcc atccacaata atgtcacctt
    84421 cagttagtcc atcaaaagaa gcaattaaat ttagtagtgt agacttccct gacccagact
    84481 cacccataat tgcaacaaat tcaccagctc caactgataa attcatttga ttaagtgctg
    84541 tcgtagcatt caaaccttta ccataaacct tttttacatg tttcacttct agcaacatgg
    84601 catgcacctc atttctttgt tagttctata ttaaaacaaa aaaatactaa taacatttaa
    84661 attagtgaaa aaatgttact agtatgctta caatattgaa agtttttatt catctggaaa
    84721 ttgaatcgta aatgttgtac cctcattttg tttagatacg actgaaacag gatggtttgt
    84781 gtgagttgaa atttgtttta cgataaataa gccaatccca cttgagttac tttgacgctg
    84841 gccattataa cctgaatagc ccttatcaaa tattttaggc aagtccgctt cactaatacc
    84901 gataccatta tcttttacgt ataattgatt ggattgctca tcaaattcaa tccatatatc
    84961 tttacctctc gcatacttaa gtgcattatt tattaattgt tctatcagta aagaggtcca
    85021 tctaacgtca gttaatactt cgtgatgaca aggttcataa tggatttttg ttttttgatc
    85081 aataaactgt attgaatatt tcataataat tgggcgaatg atattgttaa tcgaaatttt
    85141 agtgacagaa atatcagaag tttcatttaa taactttaaa taactaagtg ctaaacttgt
    85201 atagttatca atttgaataa cctcttgacg aacacgattc acaacattag gctcatctct
    85261 ttcaagtaac agttgtgctg cagtaatggg tgttttcatt tgatgtaccc atgttaaaaa
    85321 gtagctctct acatcatttt tatattcaat ttgttcattt ttaagctgat acaacgcatt
    85381 ttctaagttt tcaatttgtt gttgttggct tatagttttt acaaaactta aatatttaat
    85441 acctatgaat atgagtaata ataataaaat aacgccaagt gctagtaaat atgcttctaa
    85501 tggcaaatga tacaggtaaa acattaagcc aaataaagca aaaataacta tgactattgc
    85561 tatttcctga gtaatacttt taagaaaggt cattgcattc accctatact ttatatccga
    85621 catttttctt tgtaataata aaatcattaa cgccaatagt atttaatttt ttacgcaggc
    85681 gcgtcatgtt aacagctaat gtgttatcat ctatgaagtt ttctgattcc caacattttt
    85741 caattaaagc agttctactt acatatttat cttcattttg aaataataac tttaatattt
    85801 gtaattcagt caaagatagc tgtatgtttt gttcttgata cacgactttt gcttcatcta
    85861 atattaatgt acaaccttta accgtcaatg aatcattagc tactgacaag tcataagttc
    85921 gtctcaataa tgcttgaatt ttggcaatcg ttaatgacaa gttaaatggt ttttcgataa
    85981 aatcatctcc ccccatttgt attgccataa tttggtccat attatcaata cgggaactaa
    86041 taaatataat tggcacatta gatgtttttc ggatttcttg acaccaatga aaaccattta
    86101 acgttggcaa attaatatct agcaatacaa gctgaggttg atgttggtta aagatagaca
    86161 gtatatcatc aaattgttca acgacaataa caccgtaatt ccatttttta agttcagatg
    86221 ctaaactttc tgctataaca aaatcatctt caacaattaa tattttcatc tataatcttc
    86281 ttccttcaat tgaattaccc tttgttatct ttttgttctt tagcctgttg caatgacggc
    86341 tcttgatttg aactttccgt gtcatttgta ttttgattta gtgtatttgc tttttctttc
    86401 tcgagttgct ttttcttcgt acgtttacgc tcctctaaaa tagacaacac tataaagaat
    86461 atgattaata atggagataa cattactagt atcatttttc cacctcaaat tatatttaat
    86521 tctatatttt cacacgcttc tattctaacg ctttctataa caaattgtca tatatattca
    86581 ttttttgtta ttctaaatca ctctgtttac atatttttaa ctaaaactac ataattgctc
    86641 aaaattcctc ttttacactg aagacatagt catatactca ggaggatgtc ttatgttatc
    86701 agttaaaaaa gccacaaaat ttgcagttgc taaccttatt atcgtgtcaa cggtttttag
    86761 ttctgtcaca cctgcaattg cgcaatccga taaaagttct aaagatgaaa gtttcgcggt
    86821 tggtaatact aaaaatccaa aaaatgttat ctttttagtt ggcgatggca tgggtccatc
    86881 atttaacaca gcttatcgtt attataaaaa tgatccgcat gctaaagaaa tgacccctac
    86941 tgcttttgat tcatatttaa aaggcacaaa tcgtacatat tcaaatgacc caaaacagaa
    87001 tattactgat tctgcagctg gcggaacagc ctttagttct ggtcataaga catataatgg
    87061 tgccatcggt gttgacagta acaagcagaa agtaaagacg gtgcttgaac gtgcaaaaga
    87121 aaaaggtaag tcgactggtc ttgtttcaac agctgaatta acagatgcaa caccagcagc
    87181 atatgctgca cacgtgactt ctagagatga taaaaacgaa atcgctaaac aattttataa
    87241 agataaaatc aacggaaagc ataaagttga tgttttatta ggcggcggcg ctaaatattt
    87301 cggtaaaagt aatggcaatt tagataaaaa attcaaaaat gatggttacg accttgccac
    87361 aaacagtaag gagttatcaa aatctgataa agataaagtt cttggtttgt ttgcagataa
    87421 aaatatgcca cttgcaatag atgcttctaa agatgaacca tcattagctg atatgcaaca
    87481 aagtgcactt agtaagcttg agcgtaataa aaaaggattc tttttaatgg tcgaaggtgc
    87541 ttctatagat aaatcagcgc attccaacga tattacaggc gttatgtctg aaatggaagg
    87601 ttttgaaaaa gcattcgatg acgccattca atatgctaag aaacataaag atacacttgt
    87661 cgttgcaaca gcagatcact ctactggtgg tctaacaatt ggtaaagata aaggatacga
    87721 atggaatcct caaccgatta aatcgatgaa acactctgga tcatatatga cagaaaaaat
    87781 ggctaaaggt gaagaccctg aaaaagttat taatgaaggt tatggctttg aattttcaca
    87841 agatgatatg aaaaaggtta aaaaagagaa taaaaagcta aaaagactac ttaaaaaaga
    87901 aaaagacgaa aaatccgcaa aagtagaaac gcaaacaaaa gccctacaac acgctattca
    87961 gaagccagta aatgataaat cttatacagg gtggacttcc gatggacata caggcgagga
    88021 tgttaatacc tacgcttatg gacctagatc agaatcattt agtggtaata tcgaaaatac
    88081 agaaagcgca aaaataatat ttgatatatt caagtaaagc ataaaaaaat tcccgagcac
    88141 tataacaagg tgttcgggaa ttttttatgg tttaatttag tactcaatgt taggtgcttg
    88201 gttaacacgt tcttggtatt ttttaaatgc gaagtataaa ccgccaacta atgttaaaat
    88261 aactgctaat tttttcatca ttcaaactcc tttctaagat gtgttgaaac tgagttcaat
    88321 taaattatat gtttttatta tacacttttt gacatatttt ttaaatttaa gaatgcgaag
    88381 atttttaaca tttctgatgc tagctttctt tttcatcttt caaatgctta ataaaatcaa
    88441 agccaggtcg ccctttagcc aatgcttcca tcacatcata ccaatccgaa ccgacttgtt
    88501 gatatatatt ttgcaataat tcatcttgcg ccttgttcaa ttcagcaatg agttgatcgc
    88561 ctttatccgt cgaatataat tttttgacac gtcgatcagt ctctaaaact ttttcaataa
    88621 tgagaccttg ctcttttaat ttttgaagtg ttgcatgtga tccttgttta gaaatttcta
    88681 atatttctag taatgattta atagtaatac caggtaattt attgataaaa aacaaaaaac
    88741 gatgatgctg acggctcata ccatacttct ctataatttc atcagcggta gtaataaacg
    88801 ttttatatgc aaaataaaac aacatatgct cataatcgtt tttattggtc gacatgatga
    88861 tgactccttt caaagcgtta acctcattat aataaaattt ataatgtaag tcatacaaat
    88921 gatgataaaa ctataagcta aatctaagtt tcacgagaaa catttttgcg tatcagcaaa
    88981 ttgcgccaat catattaata caaatttaat ttaaacactc agaaatgtta gtcgtgcgta
    89041 aatagtttgt atctctcact ctgtgtattt aatgcaacca catattttcc aggaaataat
    89101 taatcattca ccatccatgt tatagcacgc ttaatcgctt ggtcccaata tgcataatca
    89161 tgatctcctg gtccatcttc aaattgataa ggaacatttt tacgtgataa ataatcgata
    89221 aaatctaagt tgtcttgata taaaaagtct tgtttaccac acataatgag caattttgga
    89281 atttgtttat cttcagctac agctttgtct agcaaataat acggatcatg ttcagttcct
    89341 ttaacacttg aaagattgcc aattatggcc tcttttgaaa aatcattcca ctctagatcc
    89401 attaaatttt gcgcttcaaa cacagcagat aatggtacag ctttggcaaa tttatcccct
    89461 tgtgttaatg caaatttaat tgtgccatat cctcccatag agtgacctgc tataaaattg
    89521 tcatcacgct ttttggaaag tggaaatatt tgatgaacat aatcatatac ttccaaaata
    89581 taatcataat agctatgacc atatgccatg ttagcatatg cgctatgatc cacattgggc
    89641 ataatcacag ctaatttgtg ttcattcgca tacctttcta tgcttgtata tctcatatat
    89701 gtcgtttcat cacttgataa tccatgtaac aacattaaag tttttaatgg tttagcagtt
    89761 atatcgttat taaagaagct ttgatcttcc ggtaaaatga ctgtcaaatt ttgatgcata
    89821 ccaattgttg gtgaatgata gtttaatgaa atataagcca tacgtcatga cccctttcta
    89881 attctactct atcaacattt tacgcttaat caattcactt taaaatcatt ttcaacaaaa
    89941 aaaccgaata caaatgtatt cggcctaaaa aagtatttac gctttttctt tatgatcttg
    90001 cttgcgtttt ctaaacaata gtaatgatcc taataatgcc atcattgcac caaataaagt
    90061 tgcatttgtg ttttcgctct tatctcctgt ttctggtaaa gcatcagttt tgtgttgttt
    90121 tgatacctta ttagaatggt ttacttcacc tttaggattt gatggtgctt tctgttcatt
    90181 atttggtggt gtaactcttg aatcggagtc actatctgaa tccgagtcgc tatccgagtc
    90241 tg
//
LOCUS       DAMZLE010000002        73710 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMZLE010000002 DAMZLE010000000
VERSION     DAMZLE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 73710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.002"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            141..1139
                     /locus_tag="POB92_000093"
     CDS             141..1139
                     /locus_tag="POB92_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6577621.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1371..2252
                     /gene="rbsU"
                     /locus_tag="POB92_000094"
     CDS             1371..2252
                     /gene="rbsU"
                     /locus_tag="POB92_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDI6577622.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2267..2671
                     /gene="rbsD"
                     /locus_tag="POB92_000095"
     CDS             2267..2671
                     /gene="rbsD"
                     /locus_tag="POB92_000095"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI6577623.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2699..3613
                     /gene="rbsK"
                     /locus_tag="POB92_000096"
     CDS             2699..3613
                     /gene="rbsK"
                     /locus_tag="POB92_000096"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI6577624.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKSTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3711..3845)
                     /locus_tag="POB92_000097"
     CDS             complement(3711..3845)
                     /locus_tag="POB92_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577625.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3864..4625
                     /locus_tag="POB92_000098"
     CDS             3864..4625
                     /locus_tag="POB92_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6577626.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPITIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(5118..6554)
                     /locus_tag="POB92_000099"
     CDS             complement(5118..6554)
                     /locus_tag="POB92_000099"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6577627.1"
                     /translation="MTKLPRNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITKSIDQNHYYPNHEGIDFYHRYKEDIALFNEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQDIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6570..7361)
                     /locus_tag="POB92_000100"
     CDS             complement(6570..7361)
                     /locus_tag="POB92_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6577628.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YIPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVRIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDRVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7510..8214
                     /locus_tag="POB92_000101"
     CDS             7510..8214
                     /locus_tag="POB92_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6577629.1"
                     /translation="MLKYEHIAKQLNAFIHQSKFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8322..9023)
                     /gene="lrgB"
                     /locus_tag="POB92_000102"
     CDS             complement(8322..9023)
                     /gene="lrgB"
                     /locus_tag="POB92_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDI6577630.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9016..9459)
                     /gene="lrgA"
                     /locus_tag="POB92_000103"
     CDS             complement(9016..9459)
                     /gene="lrgA"
                     /locus_tag="POB92_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDI6577631.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPGSV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9572..10312)
                     /locus_tag="POB92_000104"
     CDS             complement(9572..10312)
                     /locus_tag="POB92_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDI6577632.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10315..12069)
                     /locus_tag="POB92_000105"
     CDS             complement(10315..12069)
                     /locus_tag="POB92_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6577633.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12313..12987)
                     /gene="scdA"
                     /locus_tag="POB92_000106"
     CDS             complement(12313..12987)
                     /gene="scdA"
                     /locus_tag="POB92_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDI6577634.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13131..14852)
                     /gene="tarS"
                     /locus_tag="POB92_000107"
     CDS             complement(13131..14852)
                     /gene="tarS"
                     /locus_tag="POB92_000107"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6577635.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     TLLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(14885..16573)
                     /locus_tag="POB92_000108"
     CDS             complement(14885..16573)
                     /locus_tag="POB92_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6577636.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16595..17620)
                     /locus_tag="POB92_000109"
     CDS             complement(16595..17620)
                     /locus_tag="POB92_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI6577637.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17613..18329)
                     /locus_tag="POB92_000110"
     CDS             complement(17613..18329)
                     /locus_tag="POB92_000110"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDI6577638.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(18605..19774)
                     /locus_tag="POB92_000111"
     CDS             complement(18605..19774)
                     /locus_tag="POB92_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6577639.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDRILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
     gene            complement(20800..22494)
                     /locus_tag="POB92_000112"
     CDS             complement(20800..22494)
                     /locus_tag="POB92_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6577640.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWIHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(22516..23541)
                     /locus_tag="POB92_000113"
     CDS             complement(22516..23541)
                     /locus_tag="POB92_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI6577641.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LPSSYFRSSGYDGFMQDYVVMAHDRIVPLPNAIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKINHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(23534..24250)
                     /locus_tag="POB92_000114"
     CDS             complement(23534..24250)
                     /locus_tag="POB92_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDI6577642.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDYIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAEKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSAQKEILSDACKIIVESGHPVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(24778..25821)
                     /locus_tag="POB92_000115"
     CDS             complement(24778..25821)
                     /locus_tag="POB92_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6577643.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(25845..25991)
                     /locus_tag="POB92_000116"
     CDS             complement(25845..25991)
                     /locus_tag="POB92_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577644.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(25993..27048)
                     /locus_tag="POB92_000117"
     CDS             complement(25993..27048)
                     /locus_tag="POB92_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6577645.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(27066..28325)
                     /locus_tag="POB92_000118"
     CDS             complement(27066..28325)
                     /locus_tag="POB92_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI6577646.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(28552..28830)
                     /locus_tag="POB92_000119"
     CDS             complement(28552..28830)
                     /locus_tag="POB92_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6577647.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(28853..29320)
                     /locus_tag="POB92_000120"
     CDS             complement(28853..29320)
                     /locus_tag="POB92_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6577648.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(29305..31401)
                     /locus_tag="POB92_000121"
     CDS             complement(29305..31401)
                     /locus_tag="POB92_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDI6577649.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQLEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRIHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(31532..31628)
                     /locus_tag="POB92_000122"
                     /pseudo
     CDS             complement(31532..31628)
                     /locus_tag="POB92_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31738..32673)
                     /locus_tag="POB92_000123"
     CDS             complement(31738..32673)
                     /locus_tag="POB92_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6577650.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            33035..34564
                     /locus_tag="POB92_000124"
     CDS             33035..34564
                     /locus_tag="POB92_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6577651.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGILMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(34884..35837)
                     /locus_tag="POB92_000125"
     CDS             complement(34884..35837)
                     /locus_tag="POB92_000125"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI6577652.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(36148..36255)
                     /locus_tag="POB92_000126"
     CDS             complement(36148..36255)
                     /locus_tag="POB92_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577653.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            36410..37555
                     /locus_tag="POB92_000127"
     CDS             36410..37555
                     /locus_tag="POB92_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDI6577654.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSEKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            37581..37754
                     /locus_tag="POB92_000128"
     CDS             37581..37754
                     /locus_tag="POB92_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577655.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            37911..38267
                     /locus_tag="POB92_000129"
     CDS             37911..38267
                     /locus_tag="POB92_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDI6577656.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            38465..39940
                     /locus_tag="POB92_000130"
     CDS             38465..39940
                     /locus_tag="POB92_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6577657.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANAKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(40254..41396)
                     /locus_tag="POB92_000131"
     CDS             complement(40254..41396)
                     /locus_tag="POB92_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDI6577658.1"
                     /translation="MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMLLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            41837..43414
                     /locus_tag="POB92_000132"
     CDS             41837..43414
                     /locus_tag="POB92_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6577659.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDGYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTERAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            43440..44945
                     /locus_tag="POB92_000133"
     CDS             43440..44945
                     /locus_tag="POB92_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDI6577660.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIER
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            45057..46268
                     /locus_tag="POB92_000134"
     CDS             45057..46268
                     /locus_tag="POB92_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDI6577661.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            46455..48716
                     /locus_tag="POB92_000135"
     CDS             46455..48716
                     /locus_tag="POB92_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDI6577662.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQTFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDEIGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            48746..49930
                     /locus_tag="POB92_000136"
     CDS             48746..49930
                     /locus_tag="POB92_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDI6577663.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALLTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYEAQRDGRFNDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            50244..50351
                     /locus_tag="POB92_000137"
     CDS             50244..50351
                     /locus_tag="POB92_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577664.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(50533..52433)
                     /gene="coa"
                     /locus_tag="POB92_000138"
                     /pseudo
     CDS             complement(50533..52433)
                     /gene="coa"
                     /locus_tag="POB92_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744066.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
     gene            52623..52967
                     /locus_tag="POB92_000139"
     CDS             52623..52967
                     /locus_tag="POB92_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDI6577665.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            complement(53131..54894)
                     /locus_tag="POB92_000140"
     CDS             complement(53131..54894)
                     /locus_tag="POB92_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDI6577666.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(54932..55126)
                     /locus_tag="POB92_000141"
     CDS             complement(54932..55126)
                     /locus_tag="POB92_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577667.1"
                     /translation="MRQLLQWLKEDVFVSSYADKECDVLLKVGFQYHHLADVEWVIIC
                     YLHMNMSLFKFLLTLSNEKY"
     gene            complement(55230..55985)
                     /gene="pflA"
                     /locus_tag="POB92_000142"
     CDS             complement(55230..55985)
                     /gene="pflA"
                     /locus_tag="POB92_000142"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDI6577668.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(56008..58257)
                     /gene="pflB"
                     /locus_tag="POB92_000143"
     CDS             complement(56008..58257)
                     /gene="pflB"
                     /locus_tag="POB92_000143"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6577669.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            58845..59813
                     /locus_tag="POB92_000144"
     CDS             58845..59813
                     /locus_tag="POB92_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6577670.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            59810..61366
                     /locus_tag="POB92_000145"
     CDS             59810..61366
                     /locus_tag="POB92_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6577671.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRGLLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            61359..62117
                     /locus_tag="POB92_000146"
     CDS             61359..62117
                     /locus_tag="POB92_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6577672.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(62476..63855)
                     /gene="uhpT"
                     /locus_tag="POB92_000147"
     CDS             complement(62476..63855)
                     /gene="uhpT"
                     /locus_tag="POB92_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDI6577673.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            64088..64582
                     /locus_tag="POB92_000148"
     CDS             64088..64582
                     /locus_tag="POB92_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDI6577674.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(64943..65911)
                     /locus_tag="POB92_000149"
     CDS             complement(64943..65911)
                     /locus_tag="POB92_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6577675.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(65966..67006)
                     /locus_tag="POB92_000150"
     CDS             complement(65966..67006)
                     /locus_tag="POB92_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6577676.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(67031..68107)
                     /locus_tag="POB92_000151"
     CDS             complement(67031..68107)
                     /locus_tag="POB92_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6577677.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIMLESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(68181..69020)
                     /locus_tag="POB92_000152"
     CDS             complement(68181..69020)
                     /locus_tag="POB92_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6577678.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(69022..70290)
                     /locus_tag="POB92_000153"
     CDS             complement(69022..70290)
                     /locus_tag="POB92_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6577679.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(70293..71564)
                     /locus_tag="POB92_000154"
     CDS             complement(70293..71564)
                     /locus_tag="POB92_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6577680.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKRVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(71577..72674)
                     /gene="ugpC"
                     /locus_tag="POB92_000155"
     CDS             complement(71577..72674)
                     /gene="ugpC"
                     /locus_tag="POB92_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDI6577681.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(73057..73635)
                     /locus_tag="POB92_000156"
     CDS             complement(73057..73635)
                     /locus_tag="POB92_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDI6577682.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
BASE COUNT        23523 a        13753 c        11678 g        24756 t
ORIGIN      
        1 atactatagg cgtctgtttg tagacaataa aagtcattca cgtcttcata tgtcatcaaa
       61 tgtttatcat gatatgatga atataataat cgggtatata actgtatgat taattacaca
      121 ataaagagaa ggaatataat atgaaaaaag tgtcaattaa agatgttgct agagaagctg
      181 gtgtatcagt tacaactgtg tcacatattt taaatcataa tgatagtcgt ttttccgcaa
      241 caacgataaa aaacgtacat gctgtttcag aacgtttagg ctatgcccct aataaacatg
      301 caaaacaatt gcgcggcagt aaaattcaaa ctattggcgt cattttgcct agcttaacaa
      361 atccgttttt ctcagcactg atgcaaagta ttcatgacca taaaccatct gatgttgatt
      421 tatgcttttt aacatctaca gcaactgatt tgtatgacaa tattaaacat ttaattgatc
      481 gaggtattga tggattaatt atcgcacaat acatttcatc cccggacgcc ctaaataatt
      541 atctaaagaa acatcatgta ccttatgtcg tactggatca aaatgaccat caaggctata
      601 cagattttgt tcggacaaat gaatatcaag gcggacaact tgcagcacaa catttaatag
      661 aactcggtca caacaatatg atgattgttg caccatatga catgatggcg aatatgtcga
      721 ctcgtgtcgc tggatttgtt gatactttgc gcgcgaatca attgccagaa ccacaaatcg
      781 tccatactga attatctaag cgcggtgggc taaccattgt tgatgacatc atggttcaat
      841 ctgccactgc aatcttcgct attaacgatg aactcgctat tggcatttta cgaggactaa
      901 ttgaacatgg catcagtatt ccgaaagata tctcattaat aggttatgac gacattgatt
      961 atgcagcgta cgtctcgcca cctttaacta ctgtggcaca acctataact gatattggca
     1021 aaacatcttt aaccttatta cttcaacgat tacagcactt agataaatcc attgatatga
     1081 ttgaattacc aacgacttta aaaattcgtg caacaactgg ctatcatctt tcaaactaac
     1141 tacgtatctt ccgaaatata ctcatcattg ttatgccctt agcgttgctt taatgctgag
     1201 ggtttttaat cataattatt tcactaagaa attaaaataa taatgtatga atttttaaat
     1261 atgatttaaa cgttttcatt ttttatgaaa acgcatgcat tttacaaata aaaatggtac
     1321 gatggcactg gtaaaacgtt ttactaaaaa caaatcgtga ggtgtataac atgagcattg
     1381 ttgcattact tatcgggtta ggccccttaa ttggctgggg cttcttccca acagtcgctt
     1441 caaagtttgg tggtaaacct gtacatcaaa ttatcggtgc tactgtaggt acgttaatct
     1501 tcgctattat attagccgta gtcacatcaa gtggcttccc tactggaacc aatttgctat
     1561 tcgccttatt atcaggtgca ggatggggat tcggacaaat cattacattt aaagcgttcg
     1621 aattagtcgg ctcatctcgt gccatgccag tcacaacagc attccaatta ttaggcgcat
     1681 ctttatgggg tgtctttgca ttaggaaatt ggccaggcat tggtcataaa atcattggat
     1741 ttacagcttt agtcgttatt ctaattggag cgcgtatgac agtttggagt gaacgcaaag
     1801 aagcaagtaa cgccaaaaat ttacgtcgtg cagtggtact tctgttaatt ggtgaatttg
     1861 gatactggtt atattcagct gcaccgcaag caacttctat tgatggccta actgcctttt
     1921 tacctcaagc aatgggtatg gtaattgttg cagtcattta tggctttatg aatatgaaag
     1981 cagagaatcc attccgtaat aaaattacgt ggttacaaat tatttcaggt ttcttctttg
     2041 catttggtgc tttaacatat cttatttcag cacaacctaa tatgaatggt ttagcaactg
     2101 gatttattct ttctcaaaca tccgttgtgc ttgctacatt aactggtatt tatttcttaa
     2161 aacaacataa aacgtcaaaa gaaatggtta ttacaatcat cggcttagta ctcattttag
     2221 tagccgcttc tgttacagta tttataaaat aaggagtgta gatgtcatga aaaaatcagc
     2281 tgttttaaat gaacatattt caaaagcaat cgcgacaatt ggtcattttg atttattaac
     2341 gattaatgac gctggcatgc caattccaaa tgatcatcgt cgtatcgacc tagctgtaac
     2401 taaaaactta ccacgcttta ttgatgtctt agctacagtg ttagaagaaa tggaaatcca
     2461 aaaaatatac ttagcagaag aaataaaaga acataaccct acacaattgc aacaaattaa
     2521 acaattgatt tcatcggaaa tcgaaatcat tttcattcct cacgaagaaa tgaaaagtaa
     2581 cttagctcac ccattaaata aaggtaatat tcgtactggt gaaacaacgc cctactctaa
     2641 tattgcatta gaatcgaatg ttacttttta aaagttataa cttgaaagga gcatacacat
     2701 gaccaacaaa gttgttattt taggttcaac gaatgtcgat caatttttaa cagttgaaag
     2761 atatgcacaa ccaggcgaaa cattacatgt tgaagaagca caaaaagcat tcggcggagg
     2821 taaaggtgcc aaccaggcta ttgccactgc acgtatgcaa gcagacacaa catttattac
     2881 taaaattggc actgatggcg ttgctgattt catcttagaa gattttaaag cggctcatat
     2941 tgatacatca tatattatca aatcaactga agcaaaaaca ggccaagcct ttatcactgt
     3001 gaatgcagaa ggacaaaaca ccatctatgt ttatggtggt gcgaatatga cgatgacacc
     3061 tgaagatgtt attaacgcaa aagacgctat aatcaatgca gactttgtcg ttgcacaatt
     3121 agaagtaccc atcccggcta ttatatctgc atttgaaatt gccaaggcac atggtgtgac
     3181 gacagtatta aatcctgcac cagcgaaagc attacctaat gaattattat cattaatcga
     3241 tattattgtg ccaaacgaaa cagaagccga attgttatct gggattaaag taactaatga
     3301 acaatctatg aaagacaatg ccaattactt tttatcttta ggcattaaga ctgttttgat
     3361 tacgctaggt aagcaaggta catattttgc tactaaaaat caaagccaac acatcgaagc
     3421 ttataaagta aatgcgattg atacaactgc tgcaggcgac acatttattg gtgcatttgt
     3481 cagtcgctta aacaagtcgc aagataactt agcagatgct attgattttg gtaataaagc
     3541 gagctcactc actgtacaaa aacacggcgc gcaagcatct attcctctac tagaagaagt
     3601 aaatcaagtt taaatgaagc aaacacagct atgatatgaa ggtttagcat ataacatgca
     3661 acattcgtat atcatggctg tgctttttta tctttataaa acatcatcta ttagaaataa
     3721 ttttccacta aacctatgct tgttgactca tgtttagtta taaatgaagt gacaattttt
     3781 tgtaatcttt ttaacttcca aattattgca tataaatatg ctatattaat gataataatt
     3841 atcaattaaa aggaggttat gctatgtcta aagaagctgg tcatacattt ttagctaaat
     3901 taggaaaaac tcgtctacgc cccggtggta aagaagcaac agattggtta atacaacaag
     3961 gggcattttc acaagataaa caagtgttag aagtggcatg taatatgtgc acaacatcta
     4021 tttatctagc tcatacatat ggctgtcaca ttcaaggcgt tgatataaat aagaaagcat
     4081 tagaaaaagc acaggaaaat atttcagcag caggtcttga atcatatatt caagttcaac
     4141 aagcgaatgc tgttaaattg ccctttgatg acaatcaatt cgatatcgtt ttaaatgaag
     4201 caatgttaac aatgttaccc atcaccataa aggaaaaagc attacgcgag tactaccgag
     4261 tcttaaagcc tgggggtatc ttgttaacac atgatattgt catcgttaat gaatcacatg
     4321 ccacacatgt agttaaatca ttatctgcag caattaatgt caatgtctca ccgcagacga
     4381 aacttggctg gttagattta tataatcaag ctggttttaa tcatgtgcat tatcatactg
     4441 gtccaatgag tttaatgaca ccaaaaggtt taatttatga cgaaggtatt gttggaacta
     4501 taaagattat caacaatgct ttaaaaaaag aaaatcgacc aatgttttgt aaaatgttta
     4561 aaacgatgac taaattgcgt aaagatatga attatattac ttttgtcgct aaaaaagagg
     4621 actaaatata atgccactaa ccgtactttg tatctatgtt tgactgctac tttaatttct
     4681 ttgtgacact aatcatctac ttaacaatat cgttatcgtt gattagtaag tcatcaattt
     4741 tggttaaaga ctttcataaa cactcaaaca ttaacactat acatacttag ttagtggcat
     4801 tattttttta aaaattttaa catcacggga ttggaacaga aatgatattt tcgcaaaatt
     4861 tattcctgtc cttccccaac ttgcacatta ttgtaagctg actttccgcc agcttctgtg
     4921 ttggggcccc gccaacttgc acattattgt aagctgactt tccgccagct tctatgttgg
     4981 ggccccgcca acttgcacat tattgtatgc tgactttccg ccagcttcta tgttggggcc
     5041 cgccaatata acactgtagg tcctactata ttaatttatg tctcgggctc taaccctaaa
     5101 atacttataa tataccttta taatttatct ccatttgatg ctatcacttg ttgataccaa
     5161 tagaacgaat cttttttcaa gcgttccatc gtgccatgac catcattatc tcgatcaaca
     5221 tatattaaac catagcgttt cttcatttca ccggttgtaa atgaaacaat atcaatgatt
     5281 ccccacggtg tataaccgat taaatctaca ccatcttgat caactgcttc tatcgctgct
     5341 gtaatatgtg cttttaaata ttcaatgcga taatcatcat gaatatgtcc atctaccact
     5401 tcatcaactg caccaaaacc attttccaca ataaaaagtg gtaactgata acgatcatat
     5461 aacacattca atgtatatct taagccatct ggatcaatcg cccaacccca atcactcgtt
     5521 gcgatatgcg gattctccac agaatgattc aaaccaccgt tgacgatgtt gttttcaact
     5581 gtagtatcaa catcatgttt tacagccgta gacatgtaat aactaaagcc aatataatca
     5641 accgtgccct gctgtaaaat actgtcgtcc ccttcttgcc atccaacatc atatcctttt
     5701 cgtgccaaca tttttttagc atagcttgga taataacctc tcacttggac atccgggaaa
     5761 aagaaacgta agcgattcgc aatttgtgct tccatcatat ctttcggatg acacgaatat
     5821 ggataaatgg gtacatgtga aatcattgtt ccaatcttaa acttcggatt aatctcttta
     5881 ccaagacgaa ctgctaaagc actggctaaa agttcatgat gtgctacttg atacaagact
     5941 tcttcaggat tatcattttc tgtcaatgct accccagaat tcgtccataa aaagatagga
     6001 tttgatgtgt ccatctgatt attaatttca ttaaacgtca tccaatatgt cactttatct
     6061 ttatatcttt caaatacaac acgcgcaaaa tgcacaaaat aatcgacaac ttctctattt
     6121 ctaaatccac cataatgttt cgctaaatga agtggcatct caaaatgtga taacgtcaca
     6181 acaggttcaa tatcttgtgc aattaattca tcaaagatac gatcataaaa ggcgagtcct
     6241 tcttcgtttg gcacatcttc atccccattc ggaaagatac gtgtccacgc aatcgacgta
     6301 cgtaaacatt tcaatcccat ttcattaaac aaggcaatat cttccttata acgatgataa
     6361 aaatcaatac cttcatgatt tggataatag tgattttgat ctatagactt tgtaatctga
     6421 cgtgctttgc catgtgcacc actcgtcata acatcaatta cacttaaccc tttaccccct
     6481 ttatcatatc caccttcaaa ttgatttgcg gcaagagcgc caccccacat aaaatttcgc
     6541 ggtaatttgg tcattattac aacacactcc tagtttttaa atattttaaa aataacatct
     6601 tcagtcgtaa tgatttcatt cattgtcagt tctacttttc ctaaatcggc agagttgcta
     6661 ataacgacaa taacgtcagc attgtagcct tgttgttgta tatattgctg gtcgaattgt
     6721 aacaacgttt gccctgcttc aacacgatca ttttgcttta caaagcactt gaacccttta
     6781 ccttccaagt caactgtatt cacgccaata tgaatgacta tgtccacacc gtcttctgat
     6841 tgaatacgaa ctgcatgctt agttggtaca atcatagata ttaaaccatt gaacggtgcg
     6901 attactttgg attcttcatg agctttgatt gccaagcctt cgcctaccat tttctctcta
     6961 aaaattgaat ctcttacttc tttaagtaaa atacgacgac cagcgattgg tgctttcact
     7021 aaaattggtg ttgttacttt tacttgaggt atataacttg tatcttggct attaatattg
     7081 tcagtattct cactctcttc gacattagca ttcttaatta atcgctgtaa ttgctgatac
     7141 acttcatcga catttccatt taattttatg actaactgcg catcattttc tgctacagat
     7201 gtcacatcaa ctatttgttt cacttcattt gctgtagaag gaattgtatg ttgcatatga
     7261 attgtaatgc acttcgtgtc atacgttgca tcaacaatgt tttcgacacc acctacagct
     7321 gtaagaatgt cttgggcttg ttctttatat ttattgctca taaatattac cccctcaact
     7381 tgatagtaga atgaaaatgt atttacttac ctccaattat aattgtaccg gttcaatttg
     7441 taaacgccga tacaattata atattttgtg ctataataat tacagacaaa gtgaaacgag
     7501 gacagaatat tgttaaagta tgaacatatt gctaagcaac ttaatgcgtt tatacatcaa
     7561 tctaaattca aacccggtga taaattgcca agcgtgacgc aattaaaaga acgttatcaa
     7621 gtaagtaaga gtactatcat taaagcatta ggcttattgg aacaagatgg tttgatctat
     7681 caagcacaag gcagtggtat ttatgtgaga aatattgcag atgccaatcg tatcaacgtc
     7741 tttaagacta atggtttctc taaaagttta ggtgaacatc gaatgacaag taaggtactt
     7801 gtttttaagg agatggcaac gccacctaaa tctgtacaag atgaactcca attaaatgca
     7861 gatgataccg tctactattt agagcgatta agattcgtgg acgatgatgt tttatgtatc
     7921 gaatattctt attatcataa agaaatcgtg aaatatttaa atgatgatat tgctaagggc
     7981 tctatcttcg attatttaga atcaaacatg aaacttcgta ttggtttttc agatattttc
     8041 tttaatgtag ataaactcac ttcaagtgaa gcttcattac tacaattgtc tacaggtgaa
     8101 ccatgtttac gttaccacca gactttttat acaatgactg gcaaaccctt tgattcgtct
     8161 gacatcgtat ttcattatcg tcatgcacag ttttatattc ctagtaaaaa gtaataaata
     8221 cataaaaacg tctatatccc agttataaac tggagtatag acgttttttt acgataataa
     8281 caatggctca aattgctatt atcttgctta ggtttttcgt tttagaagaa tattgctaca
     8341 aagacaggca caactgctac aacaactaca ccaactaaca ctaaagctat acttgccatt
     8401 gattcttcta caggtcctaa ttctttggct ggtgctacac ctaatgtgtg accacttgtt
     8461 ccaagtgcta atcctcgggc aatagggtta gtaattcgga aaagcttcaa gaatttatta
     8521 cctagggcat aaataatgac accatttaaa ataactgcta atgatgttaa ttcttttata
     8581 ccaccgatac cagctgatac tggtaacgca atcgctgtag ttgctgcttg aggtaacatt
     8641 gataaaataa catcattggc aaattgtgct aacttcgcaa aagttaaaat aattaataac
     8701 gctacaactg taccgatacc aatacctccg atgatacgat gccaatgttt aacaagcact
     8761 tcacgctttt tatataacgg aatcgcaaaa cagattgttg ccggttctaa gaagaagtaa
     8821 ataatgtctc cacctatttt gtaagtctta tacggaatgc ctgttaaata gaggaaggcc
     8881 acaccaaata ccatactgac aaatagcggt gcgaataaga agaaacgatt agttttttca
     8941 aataatatgg tcgctaagaa aaatggtata acggataaca gtattccgaa gtaaggtgta
     9001 tttagtgcta agtggttaat catgagcttg tgcctcctct attttgatct tttttgtgac
     9061 tttgtcacct ttagatctcg aagtaacttt cataataatt tgtgtgacat agccagtaca
     9121 aataagtaat agtattgttg agacgattat tagtccaatg attaaaaatg gtgcttggct
     9181 aatgacacct aaagagttaa caactgagat accggctggt acgaagagta agccaatgtt
     9241 atttgttagt gtcgttccta ctttttcgac ttcgcctaac ttaacagcac cagtacataa
     9301 taatacaaat aataatacta aaccgattac tgatccaggc ataggaattg gcataaatga
     9361 ttcaattatt ttcgatacaa agagtactaa agcaattaca atgacttggt gaaaaaagtg
     9421 tgctggtttt gatgcgtctt tttgttgttt cacgaccatt gcctcctacg tttgatttaa
     9481 ctaaagtata gatggctcac ttcgatttgc gtaaatttta gtccgaaata caaaatatca
     9541 taggtaaaat gcataaaaaa aaggattact gttaaagtaa ccctatcgac gctttaaaat
     9601 ctttcataaa tgaacgtcca acttgcatct tgacaccatt tgtcaatatt accatataag
     9661 tgtagttaaa ccattgttgc acttctttaa tgtgtttcgt attaataata tatgaacgat
     9721 gaatacgtat gaaataagcg ggattcaatc gtttttcata acgattcaat ggctctgttg
     9781 tttcgtattt atgattcgtt gtatgtatgg ttgtaatacc attatgtgtg ccaatcccaa
     9841 taatattttg ttgctttaac atgtgaattt tatcgtctat ttcaacaggt aaactttgat
     9901 caaaattcgc cgacatatca ttcgcaattg cacttgcgct attatcatct ttggctttag
     9961 tcgcacgcac tttattgact gcttgttcaa tacgtttttg accaaacggt ttcaaaatat
    10021 agtctgtcgc atttaattca aatgcctgta ctgcgtattg gtcatgtgca gttgcaaaaa
    10081 taatcgcagg tggctctttc atcttttgaa tcttagctcc taattcgatc ccattttcat
    10141 ccattaaatt gacatctaaa aatataatgt catattgatt gatcagtagt gcttccaatg
    10201 tttcttttac attttctgcc tcattaattt cttcaaaacc accaatttca tttaataaat
    10261 atgttaattc attacgtgct aatggctcat catctatgat taatgctttc atatttattc
    10321 ctcctcttgt ctttcataag gaagtacaca ccaaaaagtg gtaccgctcg atgtcgattc
    10381 aaattgtaat gctgcggatt ttccaaataa tccttttagg cgtaagttta aattttctaa
    10441 agcactacca gttccagact ctgattctac agatgtttct cccaacaaat gcattttatc
    10501 tttagaaata ccctgaccat tatcttgtac aataatacgt acatgtgttg cagtttcttt
    10561 aatcactgac acgtcaatat cgttaccttg ctttcgattc gtaaacgcat gtttgatggc
    10621 attttcaact aaaatttgaa ttaaaaatgg tggtacaagc acatcgcgat atttgtcttc
    10681 aacattaata ttgatattaa atcttcctgg ataacgtgct tgttcgagtg ataagtatgc
    10741 acgcacttga cttaactctt tatctaaagt aatcgtatgt tgcttagagc cttgtaaatt
    10801 cgctctgaaa aaataactca attctaatag taactctcgt gccttttcgc tatttattct
    10861 aactaaagct gaaatcgtgt taattgaatt gaagaaaaaa tgtggactca cttgtgcctg
    10921 taatgactta atctcagcat ctttcaataa cttactttgc gtttcggctt caccaagttc
    10981 aatttggcta ctaaaaatat ttgccaatcc ttctgcaagt tgacgttcca caaaagttaa
    11041 atcattaggg tttgtaaaat acatcttcaa tgtaccgacg atagaaccat gcatctcaag
    11101 tggtatcacg atagctgctc taagcgggca attcggatga ctacaaccaa tctcttcttt
    11161 agtatgaact tctttcaact ttcctgattt caatacatct ttagacagac ttgttaatat
    11221 ttcatttgtt ggtatgtgat gatcactacc tgcacctaca tgcgataaga tttcattttt
    11281 gcttgtaatt gctacggcag atactttcat taaattttta ataatcatcg caatttgctg
    11341 tgccgattct ctattcaatc cttctttaaa atacggcaat gtctggttca tcaattgcag
    11401 tacatcatgt gtttgaacag ccttcatttg ctcctcttgc tttaatgttg aaatgataat
    11461 agacataaaa atcgccgtac cgacgctatt aacaataatc attggtagtg caattaatga
    11521 tatgaggtca accgcatatg ctttgtcgtg ggaaaatgtt aaaatgctca acatttgaat
    11581 catttccata acaattccaa tcatggcact tttcgcaata ctcgggtaac gcttgcgtct
    11641 ttgagcttgt aagccaaaat aaccagcaat tataccgata aatatagatg agataagata
    11701 aacttgtgca tccgccccac ccatatacac tctgaaaata cctgaaataa cgccaacaaa
    11761 tagacctaca aaagggccac caactaatcc tgcgacacct atcgttaata cacgtgtgtt
    11821 agctaaagat acatcatcat ctaaacggaa gtacacactt cctgacaaac tatgttgatg
    11881 atcgatgacg ataccagtta aattagacat taaggcaaac aaactgaaaa taatacataa
    11941 ttgccaacgt gctttccatg tacgtcgacg attcattaag tttttaaaat atggaatatt
    12001 catcaacaca taggccaaaa taataattaa acctacacgc tcaagtaata acattgttag
    12061 cgatagcacg atagcacctc agtaaattat aattatgttt attttatcat aatagtgcat
    12121 gtaaaattca tgaataaatt attaaaaaac acgctactat ttaattatct ataccattaa
    12181 tttttcatat caatttttat gctaagttga ccatttttct tttcaaataa tgctgttcat
    12241 atcactaaat ttctgttaag tatagcgaaa aactgctgac attactttgc caacagtttc
    12301 taatcgcgtt atttatgata cttttttaaa taaaacatga ttctctaaat gaacatgctc
    12361 atgtgttaac acttcaagtg cttttaatct ctgataaacg agtcgccacg taccacatgc
    12421 ctctatcggt ggttcatagc tagatgttaa atcgctcatt ttcactaaca attgtcccgt
    12481 tgcaatgtga tcagatacta aatcatcaat cactgtatta atatcgtcta ctacttcacc
    12541 ttgttcatat ttaattagtt ttggaaaatc aacatcatct tctttttgca tatgctctaa
    12601 catgccattt ttaaatgtat catatgtttc ttttaactcg actaaatatg gatggttagg
    12661 tccatgtact ttcgataatt tcgtcacata aggtgttaaa tttttaaatt cttctctaag
    12721 aggttcatga tatgctgctt gaatatattg aataagtgac gaaacattta aaaatttagg
    12781 gttaagcgaa cctggtgtat tcgtttgttc aacgtcattg agacgctgta ataattcgtt
    12841 caaatctaca tttttctttt ctaaggatgc tgcttctata cttacttgtc caccgcaaca
    12901 aaagtctata cctacgcttc taaaaatatc tgctgctttc ggataatctg ttactacatc
    12961 tgctactatg tcatttttat ttatcattat agacgacctc ctcattttca gtgtatacaa
    13021 tttaatgtat atgaaaatga aaatcaggga aatgtcataa tcatttcata aatagtaaaa
    13081 taaaaccctc aaatcatgtt agttcacata gatttgaggg catttattat ttattttagc
    13141 gagtaagtca tatgtgcagt gtttaactta acaatatttt tgaagtctcg cttcttaata
    13201 tctttactaa gtaagatttc cacttcgttt tctgacgttt ggcttaacat tcccataatc
    13261 atgtcgtctt ttagcacgac actagccata ccttcttcaa atacataacc acgatccaga
    13321 ccagttaaaa tgattttgtt tttacctttt aacttcaatg agattgaatc cgttaatgta
    13381 atagctgtaa agtataacga aatgttatta aagtcttttg tgaaatacgg tgttaatcta
    13441 taaaaatcat tattcaaatg aattaatgca cttgtttctg cagtatatgc atatttatta
    13501 cgttgattac caacacgcac ttcaaatact tccgtaccta tttggacttc taagaaaaca
    13561 tcccaaattt tttctttagg taaatacgct ttaatatctt ctaacataat gttaaaacga
    13621 tattgtttat tcgcattgac acttaaaggt acatacattg tgtgcttctt gtttcgtgaa
    13681 tgtaatttaa gtctgacatc ataatgtcct tcgccaatca taaattcatt taacgttagc
    13741 tctgtaacaa ttttatgatt atcaaattta aatgctgtca ttttcacttt tggcttagcg
    13801 atatcaatgc cttcaaagta tggttcgcct tcattgaatt gaatgtttaa tttaccatct
    13861 acaatatcaa aacggtagta ttgaccttga cggtaacttt cttctacagt tctatagttg
    13921 tctatatctt tcgctctcgc gtagtgcaac aatggtcgta atttactcat caccaatgca
    13981 tctacacgtt ctggaactgc ttgtataaag tctcctagag cattaatcca ttgcggttga
    14041 tcttctagtt taactttaag tgagaagcca ttcgtacgag aaaaactaaa atgacgattt
    14101 aaaaattctg ctaagatttg atctttatga gcttcttcta aatctgcatt taatatttct
    14161 acagcaatca atctcatgac ttcgtaaaag tcttcaggtg aaacatacgc actactcata
    14221 tgttcacctt cacgctttgt agcataataa tacgctttat cacttaacac actgattcga
    14281 tttgcattca aatatgcttt cattgtaaat aattgatctt ctgcactctt taattcttct
    14341 ggaaaataaa tgtcattatc ttttagtaat gttgttctat agattttagt cgggcttaaa
    14401 gtatagataa ttcttgaatt taacaaagta acttcaggtg ctgtttcttt aaacatagat
    14461 tgtggtacac cacgaccatt aacacctttc attttaatca ataagacatc ggagtgatgt
    14521 tcatcaataa atgctgctgc atcttttaaa gtctctttgt ttatatagtc atcggagtcc
    14581 acaaataata caaattcacc ttctgcttgt tttaacgcca catttctagg tttacctgga
    14641 ccaccagaat ttgtttcgag ttgacttact ttcaagttca atttattgcg atacttttca
    14701 actatttgta acgtttgatc tgttgaacag tcatcaacta caaccacttc aaattcagtt
    14761 ttcggaaaat cttgtttcgc aaggctatta agtaattctg ttatatactt ttctgaattg
    14821 tatgttggaa ctattactga aaatttcatc attatacctc tcccactttg actactatat
    14881 aaacttagct accaaataaa tttctgacta aacgctcact tgatcggcca tcttgatatt
    14941 taaaatgttt atctaagaat ggaatgactt tttctccttc ataatcttca ttgtccaagg
    15001 cgtccattaa tgcgtcaaat gattgcacaa ttttacctgg aacaaatgat tcatatggtt
    15061 cataaaaatc acgcgtcgta atataatctt ctaaatcaaa tgcatagaaa atcattggct
    15121 ttttaaatac tgcatattca tatattaaag atgaatagtc actaattaat aaatctgtta
    15181 tgaacagtat atcattaact tctctaaagt cagaaacgtc aacaaaatat tgtttatgtt
    15241 tgtctgcaat attaagtcta tttttcacaa atggatgcat tttaaataat acaaccgcgt
    15301 tatttttttc gcaatatctt gctaaacgtt caaaatcaat tttgaaaaat gggtaatgtg
    15361 ctgtaccatg accactacct ctaaatgttg gtgcgaaaag aatgactttc ttacctttaa
    15421 taattggtaa ttcatcttcc atctcttgtt tgatctgtgt cgcataagct tcatcaaata
    15481 gtacatcagt acgtggaaca cctgtaggca ctacattttt ctctttaata ccaaatgctt
    15541 cagcgtagaa tggaatatcg gtttcagatg atacataagc ttttgtatag ctacgatgat
    15601 ttaatgaatc aataaatggt ccaccctttt taccagtacg actaaagcca actgttttaa
    15661 aggcaccaac ggcatgccat acttgaataa cttcttgaga acgtctaaaa cgcactgtat
    15721 aaatcaatgg gtgaaagtca tcaacaaaga tgtagtctgc cttcccaagt aaatatggca
    15781 atctaaactt gtcgatgatg ccacgtctat ctgtaatatt cgctttaaaa acagtgtgaa
    15841 tatcatactt tttatctaaa ttttgacgta acatttcgtt atagatgtat tcaaagtttc
    15901 cagacatcgt tggtctagag tctgatgtaa acaacaccgt attccctttt ttcaagtgga
    15961 aaaatttcgt cgtattaaat atcgctttaa aaataaattg tcttgtatta aatgattgtt
    16021 tgcggaaata cttacgtaat tctttatatt tacgaacgat ataaatactt ttaacttccg
    16081 gagtcgttac aacaacatca aggacaaatt cattaacatc gctagaaatt tcaggtgtaa
    16141 cagtataaac cgttttcttc gaaatgccgc cttttctaaa ttcttttagg taagtctgca
    16201 ataagaaatt gattttacca ttttgtgttt ctaattcgtt gtattcttct tcttgttctg
    16261 gctttagatt ttgatatgca tcattaatca catctgggtt taactgtgca atataatcaa
    16321 gttcttgctc attcactaat aagtacttat cttcaggtaa gtaataacca ttatctaaga
    16381 tagctacatt gaaacgacaa acgaattgat tcccatctat tttgacatca ttcgccttca
    16441 ttgtacgtgt ctcagttaaa tttcttaata caaaattact atcttctaaa tctaggtttt
    16501 cactatgtcc ttcaacgaat aactgaacac gttcccaata gattttatct atatatatct
    16561 tacttttaac caacgttaat tcatcctttt ctatttacat aatccatttt aatactgttt
    16621 taccccaaga tgtagacagg tctgcttcaa aagcttctgt aagatcatta attgttgcaa
    16681 tttcaaattc ttgacctttt aacaacgcta atttatctac aatatctggg tattgaatgt
    16741 ataagtctac aacatcttgg aaatcttttg aaccacttcg actactacca atcaacgtta
    16801 gtcctttttc caatactaga cgtgtattaa cttctactgg gaactcactt acacctaaca
    16861 gtgcaatgct tccttctggt gaaatgtaat cgatcatttg atttatggct gattgactac
    16921 cgcgaccacc cacacactca aatgcatgat caaatgttaa gccttcaggt attttattaa
    16981 taaagaagac atcatcaaca aatgagaagt gactcaattt ataatctgtt ttaccaaaga
    17041 catatatttt agactctgga tataatttac gtaataaaat ggctgtaatg taacctaagt
    17101 taccatcacc ccaaatacca aatgtatttt tatttgaaat agatttcttt tcaaaacgac
    17161 gaatagcatg caaacttact gttacaagct ctgtatatga aataatactt aaatcaatat
    17221 catcaggtag tggtacagct ctatcatgat ttagcaacac aaaatcttgc ataaacccat
    17281 catgtccact tgatctgaag tagctcgatt ttaaatagtt ttcagcaatg acatcgtctt
    17341 tttctgtcgg cgtattcggt accataacta cttttgtacc tttattaaac acacctttac
    17401 tgtcaaatac gacctcacca acaccttcat gaattaaaga cattggcaat ttctgagata
    17461 agacattctc atcacggcta ccagtataat atctttgatc ggcagcacaa attgacatat
    17521 ataaaggacg tacaatgaca tagtcactgt aaatatctac gttgttatac gtaacttcaa
    17581 attgtctagg tgcaactaat tgatatactt gattaatcat cggcaatacc acctcgaata
    17641 atagcattcg ctacttttaa atcgtaaggt gttgttactt taatgttata taactcacct
    17701 tttacaagtc gaaccggttt gtttgtttct acaataatct tacaagcatc agataaaata
    17761 ctcttttgct catcactcaa ctgtgcatag ctttctttta ataaattaat attaaacgat
    17821 tgaggtgttt gaccttggta catttcatta cgcactggaa ttgcatcaat cgtttgatta
    17881 tctttagatg taacaatcgt atctatagca tcaatcactg tatctactgc accgtactct
    17941 aaagcagctt gaatattttc tttaataata cgatgcgtta aaaatggtct aactgcatca
    18001 tgtgtcacaa tgacatcgtc atcgttaata ccatttgttg attcaatatg tttaacgata
    18061 ttcataattg tatcgttacg atcgctacca ccttgaatga cttcaattct ttcatcagaa
    18121 attttgaatt ttctaagtgt atctttcgta tgcgtcatcc attgttgtgg cgtcgcgata
    18181 ataatttttt caaaatcatt aattaaaata aatttttcta atgtatggat taaaatcggt
    18241 ttgttgtcta aatctaaaaa ttgtttaggt aaaggtacgt tacccattct tgagcctata
    18301 cctccagcta gaataccagc gtatttcata aaatacttcc tccattcaac tatatctata
    18361 tttaattatt taaatttcgt tgcatttttc aattgaaaac tcattttaaa atcaaaactc
    18421 taaatgtctg tgtattactt aaaattatac atattttgct tatattttag catattttgt
    18481 ttaaacctat attacattat atcagacgtt ttcatacaca aataataaca tacaagcaaa
    18541 catttcgttt attatttata tcacttaact aattaattta taatttttta ttgtttttaa
    18601 gttatcactt aaaaatcgtt tggcaaattc gttgtgacgc gtgtccatct tctaatgaac
    18661 agaatttttg ataaaatacc gttcgtgctt caatatactc atttgcagtc tcatcgattt
    18721 gttttaatgc atcaatgagt gctgtttgat tttcaacaat tggacctggc aactcttttt
    18781 tataatccat gtaaaaacct ctaagctcat cgccatattt atctaagtca tacgcataga
    18841 aaatttgcgg acgctttaat acaccgaagt cgaacatgac agatgagtag tcggtaacta
    18901 acgcatcgct gattaagtat aaatccgaaa tatcttcata atctgaaacg tctttcacaa
    18961 aatcatcatg ttcatcaata cgtgtcacaa ctaaataatg catgcgtaat aaaataacat
    19021 aatcatcatc cagcgcttga cgcaaagctt ctatatcaaa gttaacatta aattgatatg
    19081 aaccttctcg aatcgcttca tcgtcacgcc aagttggcgc gtacataatc acttttttat
    19141 ctaatggaat atttaatctt gtcttaatac cattaatata ttcagtatca ttgcgtttat
    19201 gtgataattt atcatttctt ggataacctg tttccaaaat cctatctcga ctaacatgaa
    19261 atgcatgttg aaatatcgat gtcgaatatg gattaggtga cactagataa tcccaccgtt
    19321 ggctttcttt tttaaagcca tcttggtaat tttgagtatt tgttcctagc attttaacgt
    19381 tactaatatc caaaccaatc tttttcaatg gcgtgccatg ccatgtttgt aagtacgtcg
    19441 ttcgcggtga tttatataac caatctggtg tacgtgtgtt aatcatccac gctttcgctc
    19501 ttggcatcgc taaaaaccat ttcattgaaa actttgtaac atatggtaca ttgtgctgtt
    19561 ggaatatgtg ttcatatcct ttttttacac cccatattaa ttgggcatcg ctatgttcag
    19621 ttaagtattc atataatgct ttggggttgt cgctgtattg tttaccatga aagctttcaa
    19681 aataaattag attcttgttt ggcaattttg atagtaattt aaaagtcgta tatatactat
    19741 gttctatcaa ttttttaatt gtatttttaa tcatgtcgta cctccgacgt gtttttgtaa
    19801 ttatattaat atgtatgagc aagctcattg taaccatgcc tattatagca tttcatcata
    19861 aaatacattt aaccattaca cttgtcgtta attatcatac gaaatacatg attaatgtac
    19921 cactttaaca taacaaaaaa tcgttatcca ttcataacgt atgtgtctac acatttatga
    19981 attagataac gattggatcg attattttat tttacaaaat gacaattcag ttggaacagg
    20041 ctgggacata aatcaatgtt ctatgctcta cgaagttata ttggcagtag ttgactgaac
    20101 gaaaatgcgc ttgtaacaag cttttttcaa ttctagtcag gggccccaac acagaagctg
    20161 acgaaaagtc agcttacaat aatgtgcaag ttggggatgg gccccaacac atagaatttc
    20221 gaaaagaaat tctacaggca atgcaagttg gggtgtgggc cccaacacag aaaattcatt
    20281 ttattgaatt ttacatttat gtgcaagttg ggcaaagtat cttatttttc aaagtattta
    20341 aaagtaaaat tacatgttaa tacgtagtat taatggcaag actcctgagg gagcagtgcc
    20401 agtcgaagac aggggcccca acacagaagc tgacgaaaag tcagcttaca ataatgtgca
    20461 agttggggtg gctgagacgg caccctagga agggacccgt catcaaaaat tctatttata
    20521 gaattttaca gtaatgtgcc agacgggcaa agcgaagcca ttcaatacga agtattgtat
    20581 aaatagagaa cagcagtaag ataattttca attagaaaat atcttactgc tgttttttag
    20641 ggatttctgt cccagcctct tatgcatgaa aaatcaaaag gttattctca ttgtatagtc
    20701 ctgcttctca tcatgacatg ttgctaactt cattgtcaga acccttcttg aaaactatgc
    20761 cttatgactc atttgcatgg caagtaatat atgccaacat tagcgtctaa acaaatcttt
    20821 gactaaacgt tcacttgagc gaccatcttg atatttaaaa tgtttatcta agaatggtac
    20881 aactttttca acctcataat cttcattgtc caaagcatcc attaatgcat caaaggattg
    20941 tacaatttta cctggaacaa atgattcata tggttcatag aaatcacgcg tcgtaatgta
    21001 atcttctaag tcaaatgcat agaaaatcat cggcttttta aatactgcat attcatatat
    21061 taaagatgaa taatcactaa tcaacaagtc tgtaacaaag agaatatcgt taacttcacg
    21121 atgatctgac acatcgataa agtattgtct atgttcacgt gaaatattaa gtctattttt
    21181 tacgaacgga tgcattttga ataacacaac tgcattatgc ttctcgcagt atcttgctaa
    21241 acgttcaaaa tcaattttaa aaaatggata atgtgccgta ccgtgaccat tacctctaaa
    21301 tgtcggtgcg aatagaataa ctttcttacc ttttataatt ggcaattcat cttccatctc
    21361 ttgtttaatt tgtgttgcat aagcttcatc aaatagtaca tcagtacgtg gtacacctgt
    21421 tggtacaaca ttttcttctc taattccaaa tgcttcagca taaaatggaa tatcggtttc
    21481 tgatgaaaca tatgctttcg tgtaactacg atggtttaat gaatcgataa atggaccacc
    21541 ttttttacct gtacgactaa agccaactgt tttaaaagca ccaacggcat gccacacttg
    21601 aataatttct tgtgatggtc taaagcgaac cgtataaatt aatggatgga aatcatcaac
    21661 aaaaatataa tctgccttac caagtaaata tggcaatcta aacttgtctc tccatttacg
    21721 tctatccgta atattcgcct taaataccgt tttaatatca taatcaaaat ctactttttg
    21781 gcgtagtaac tcatcatata catacttgaa attccctgat aaattcggac gcgaatctga
    21841 tgtgaataat attgttttgc ttcttttaat atgtagtaac tttgtaatat taaaaatagc
    21901 tttaaataag aaacttctac tttcaaatga agctttatga ccttgtttat gaatccagtg
    21961 tgcacttgtc gcaatgaccc ctgatttctc ttgaggtaag gtgatttcaa tatcaaatac
    22021 aaattcgtta acgtcacttg ctatttccgg agtaatggta tagaccgtat gttgtgatac
    22081 gccaccttta cggaaaactt tagcatcata cgctaataaa tagttcactt ttccattttg
    22141 tgtagtcagc tcattgtatt cttcaatttg ctcttcattt aatgccgcat aagcttgatc
    22201 aataatattt ggattaagtt gtccaatata ctctaactgg tcatgataaa caaataaata
    22261 tttatccatt ggtaaataat acccatcgtc taatattgcg acgttgaatc tacatataaa
    22321 tgtattaccg tctattttca tttcatttgc ttctaatgtt tttgtttcgg ttaaattcct
    22381 cagaacaaat tgtcctgatg ttaaatcgac accttcacta tgtccttcaa tatataactg
    22441 aacacgttcc cagtatatgt tatcaatatg tattgcttgt ttcgtttttg tcattgttat
    22501 tcccccatcg gtttattaaa tcgtccattt caatactgtt tttccccaag atgtcgataa
    22561 atccatttca aacgcttgga cgatatcttg catcgtacat acattaattt catgtccttt
    22621 taataaggct aacttttcaa ctatgtctgg gtacttacga tataaatcaa caacttgctc
    22681 aaaatcttta gagccgcttc gactactacc aatcaatgtt aatccttttt caagtactaa
    22741 tcgtgtattc acttccacgg gtaattcact tacgcctaac aaagcaatac tgccttctgg
    22801 tgaaatatgt tcaactattt gttgaagtgc aacttgactt cctttacctc caacacattc
    22861 aaatgcatga ttaattttaa gatcatctgg tatttgattt actgtaaaga tgtcatctac
    22921 aaatgaaaaa tgacttaatt tatagtctgt cttaccaaat acataaattt tagcttctgg
    22981 gtacaactta cgtagcaaaa tagcagtaat ataacctaag ttaccatcac cccaaatacc
    23041 aaagctggtt ttcaaaggta tagatttacg ttcaaatcgt tgtatagcat gataacttac
    23101 tgacactaac tctgtgtatg aaatcgtact caaatcaatg gcattaggca gcggaacgat
    23161 acgatcatgt gccatcacaa cgtagtcttg cataaaacca tcataaccac tagatctaaa
    23221 ataactagag ggcaggtaat tctcggcaat cacgtcgtgt ttttctgtag gtgtattcgg
    23281 caccattact actttcgtac ctttttcaaa tacgccttta ctatcgaata caacttcacc
    23341 aacagcttca tgaactagtg acattggtaa ttttttgcgc agtacatttt catctcttcg
    23401 acctgtgtaa tacctttgat cagctgcaca aatagacaag tataaaggtc ttacaatgac
    23461 atgattacca taaatatcaa cattattata tgtgacgtcg aactgtctcg gtgcaacgag
    23521 ttgatatact tgattaatca tcggcaatat caccttgaat aatggcattt gctactttta
    23581 aatcatacgg tgttgtcact ttaatgttgt atagttctcc acgtaccaat ttaactggat
    23641 gtccagattc gacaatgatt ttacatgcat ctgataagat ttctttttgt gcactactta
    23701 aggcgcgata actatcttgt aataatttaa tattaaatga ttgtggtgtt tggccttgat
    23761 acatttcatt ccttacaggg atactgtgta tgttctgttt atctttagac attacaatcg
    23821 tatcaattgc ttcaatgact gtatctactg caccatattt ttctgctact tcaatattct
    23881 ctttaataat acgttgagtt aaaaatggtc ttacggcatc atgagttaca atcacatcat
    23941 cattattaat tccatttaca ttgcgaatat agtcgataat gttcataatt gtttcgtttc
    24001 gatccgtacc acctgcaact actttgacac gttgatctgt aatgttatat ttttttaaaa
    24061 tatcctgtgt atgggaaatc cactgtgctg gcgttgcgat aataatctca ttaaattcac
    24121 tcactaaaat gaacttctca attgtatgga ttaaaatcgg tttattatca atatctaaaa
    24181 attgttttgg taatggcacg ttccccattc tcgaaccaat acctcctgct aaaatacctg
    24241 cataaatcat gttgtcctcc attctgtcat tacatcattt ccatttatac attactgacc
    24301 tatgcccgca cataagccta acctattgct cacttgcctc ttttattaat ccaaagatag
    24361 ttgtcacaat agtgtgataa ttttttataa aaatgtattt ttgtaactga ccattctaag
    24421 ttgttttgcc atgcagttaa tcattaactc tgacgatatt aaattgttaa aggtattaat
    24481 gtttactctt tttcaaattc attattactg ccatcatttt accatatatt ataataaatt
    24541 tatcttatta agtggctgta cttgattttc actttaaaaa ttatcaaata ttgccatctc
    24601 attttaagta tacaaaatgc aaaacaaccg attcacaagc atatttcaca caagtaaacc
    24661 ggctatttag caacgtatat tcgaagatga gttatttcga tagtatctgt agaccagacg
    24721 gcattcgcac tttcatagct ataactatac cagtgttttc gtcctcaaag gtgcatacta
    24781 ataaatcgta aacatgactt tatcaaatcg ttctttcttg ttaactaatt tatcaaatgt
    24841 ctctgggcct ttttctaacg gtaaaaaatg agaaataata ggctttacat taatatcttt
    24901 cgtcttcata taatgtaagg ttgccgtcca ctctttgccc ggaaaattac tggacaaaca
    24961 gttccaagag ccacatactg tcaactcgtt acgcagaatt ttttcaaaat gaacgcgatc
    25021 aatctcaata tcatcatatg gtattccgag taataccacc tcgccacctt ttttaggtag
    25081 cgtcaatatt tgaccaatcg taactttagc acctgatgat tctatagcta aatcgatttg
    25141 attggcgtaa tgattttcga tgaatttctc aagattttct tcttttgaat tgattgtttg
    25201 atgtgcgccc aatgatgttg caatatctag tttatgcgca tctatatcta tagcgatgat
    25261 atgtgcagca ccaaatattc gtgcccattg aatagctaac aaacctatac tgccacaccc
    25321 cattactgca acagtcatac caggttgtat attcgattta taaaacccat gcgcaacaac
    25381 ggctgatggc tcaaccattg ctgcttcaat gtaatcaaca ttgtctggaa cctttaaaac
    25441 attttgcgct ggcaatttga catattccgc gaacgatcca ggttcatatg agccaataac
    25501 gaataacttt tcacatcgtg catattcacc ttttaaacaa tactcgcatt gataacaagg
    25561 tattgctggg caacctgtca ctttgtcgcc tacattaaca tgcgtaacat cacttccaat
    25621 ggcatctaca acacctgaaa attcatgacc aaatggcata cctttaatgt atggccccat
    25681 ttttttgtat cgtgacgtgt ctgaaccaca tatgccagtc gctcgtactt taacaataac
    25741 gtcattcgca ctttcaatga ctggcttttc attatcctca taccgtaaat cttccacgcc
    25801 atataatttc aatgctttca cttgtaaatc acctcaaatt tgatttaatt cacaactttt
    25861 ttctttttaa aaatacctgt cgcaaaataa cctgcaatga caatggaatt acttacgagt
    25921 aaatgttcca tataaaaatc agtgatttgt cttaatggcc caagcataaa agttagcatt
    25981 gattctacca tattaattct ctccttcatt ttcatctaaa tcgagcggct taagtaatgc
    26041 tttaatacct tcgccagatt ttaaatgttg atacgcctcg tcccatttcg aaatatcata
    26101 tatttttgtc accaaagctt cagcatttac taaaccatcc gccataagtt gcaatgaagg
    26161 ttcccaatct gctggctttt gacttctact accaacaact gttatttctt tttgaatcac
    26221 tttttccata tcaaatggaa tttcagcatc cttaaaaata cctatttgac tgtagaaacc
    26281 ttttttgcgt aaaatatcca aaccttgtcg tgctgctgga actgcacctg aacattcaac
    26341 aacaacatct gcaccgtaac cgtctgtaat tccattgata tacgttttta agtctgtttg
    26401 ttgtaaattg actacataat ccatgtgcaa tgcttctgct ttatctaatc tgactttgtc
    26461 attgtccaat ccagttacca caacagttgc gcctttactt tttaacactt gtgctacaag
    26521 taatccgatt ggcccaggtc ccattacaac tgctacatcg cctgaattga cttgaatctt
    26581 agaaacgcca tgatgtgcac atgctaatgg ttctgtcata gctgcagact gatacgatac
    26641 ttcatctgga atatgatgca aactttcttc acgtgcaatg acataattag taaatgcgcc
    26701 atcaacttgt gttccaatac cttttcgatg gttgcataaa ttatagtctt ttgatttaca
    26761 gtattcacac tcattacaaa catagaatgt cgtttcagaa gtgacacggt caccaacttt
    26821 aaaatcttta acgtctgctc caacttcaac gatttcacca gaaaattcat gacctaatgt
    26881 cactggaaaa ttaactttat aatgcccttc ataagtatga atgtctgtgc cacaaattcc
    26941 tgcataatgt actttaatct ttactttatc atctagcggt gttgcaactt ctttatcaag
    27001 aagttctaag ttgccatgtc cttctcttgt ttttactaaa gctttcacca caaacacctc
    27061 gatttttaat tgaatagact aaatagttta aagataagat agttaacgat attaccacct
    27121 tgatcaatac ttgaaatttc agatgaacct tttggcattt gtacattcgt acctttcgcc
    27181 atatctgtga aaatgggtgc tacgtctgtt gcaatatata atgaaattgc aatcataatc
    27241 gtgcccacaa tgacagaatg aataatgttt cctcttgctg caccaacaat aaacgcgaca
    27301 acaaatggta tagttgctaa gtcaccaaaa ggtagtactt ggtttcctgg taaaataacg
    27361 gctaataaaa cagtgatagg tactaaaatt aatgctgtcg aaataaccgc tggatgacct
    27421 aatgctacag ccgcatccaa tccaatataa atttcacgtt cgccaaaacg tttatttagc
    27481 catgttcttg cagactctga aactggcatt aaaccttcca ttaagatttt taccattcta
    27541 ggcattaata ccattactgc agccattgac attcctaaat taatgatgtc tccaggtttg
    27601 taacctgcta acacaccaat acctaaacct aaaattaatc cgacaaagat agactctcca
    27661 aatgcgccaa aacgtttttg aattgtttca ggatcagcat ctaacttatt cagaccgggt
    27721 actttttgta acaatttaac taagtaaata cctggtgcat aagaaattgt acttcctgta
    27781 gcaatcgata cacctggtaa atcaaagaac tcactcatca tcggtgctgt ccaatctgct
    27841 actttcaaac agataacttg gaaaataact gctgctaata acgcttgcca aatactgcct
    27901 gatacggcat aaaccattgc tgctgtaaac gtataatgcc aaaaattcca aatatctaca
    27961 ttcatcgtct ttgtcacttt agttactagc aatacaacgt taactatgat tccgagtgga
    28021 ataataaatg ctgcgacaga tgatgcccaa gcgatagatg atgttgctgg ccaacctaca
    28081 tcaatcacat tcagactgac gcctaaattt ttaaccatcg cttgtgctgc tggccctaaa
    28141 tttttaacta ataaatcgat gactaagaaa atccctacaa aagccacacc tattgttaaa
    28201 ccagacctaa atgccgctcc aattttctgc ctaaagaata ggccaagcaa gaatatgaca
    28261 accggtaaaa taacagttgc acctaaatct aaaaatcccc ttacaaaatc agtgaagtaa
    28321 ctcatattta aaccctccct gttatatatg cattgtcacg atactttccg attgtgatta
    28381 catttgacgt tacagtcatt tcaacgacaa cccttgctac attcgactgc agtcattttg
    28441 aattacagtc actgcgtttc tatgtcatca acaatcattt gtcgtgatag tcatttatat
    28501 gcaatttgca tatattaata tgttatcgac ccacgttaca tatcaattcc gttatttttg
    28561 taactctgtt aagatttgtt gttttgtttc ttcaatacca ataccagtta agaaattacg
    28621 tgcgttgata actgggaatt tatattcttt ctttgtcatt gcagttgtaa ctaataaatc
    28681 tgcagtgtct tcataaggtc caacttctgt aattttgatt tgtttaatat ctactttaat
    28741 attgtgttcc tttgccattt cctcaattgc attatttact actgttgacg ttgcaatacc
    28801 tgcaccacac gctactaata cttgtttcat tttcaattcc tccaattaat ttttagttat
    28861 attccaaata atcattgatt agtgttgcta aaatcgtttc atctttcgtt cgcagaatct
    28921 gctccaattt ttcttcactt tgaaaaattt gcatcaactg ttgtaacaac ttaagttgat
    28981 catctacttt atccatcgct aacataaaaa cgattttcac ttctgtctgt tgatcaagtg
    29041 ttcccatttc aataaacggc acctcttttt ctagaacagc cacacctatc gttctatggt
    29101 taatatgttc gacatctgta tgcggtatag cgaccgaaca tagatgtgtt ggtaaaccag
    29161 tagcaaattc tttttctctg tcgatgactg catctttaaa cgttgacttc acgaacccat
    29221 tttgaaataa cacatctgac atttgtgaca atacggattc tttatcagtt gccgacaaat
    29281 tgagcattat attttcttta tgcactaatt gctgtcccat ccattttccc tcgcttcttt
    29341 atttgaataa ttttttaaaa tctcatttac atcagaattt ttgcgacttt gtatgatgcg
    29401 cttaattgcg tcattgtctt gcgccacatc tctcaattgt agtaacgctc ttaagtgtgt
    29461 cactttatca acagcagcaa taggtacaat aatatggatt gctgtgccat ctgacatgta
    29521 tattggttct tgtaatatca acatactcat cgctgtttta tgtacatgct tttcagagta
    29581 tgcatgtgga atagcaatat tttgcatgat aaccatatac gaatcatcaa atacctgttg
    29641 catctcatga atatatttaa tatcaataaa atgattagca actaacacat cacttgcttt
    29701 agcaatagct tcatcaatat tttcaacatg atgtattctt ttcacgtgcc ttgccggtat
    29761 caagtcagct aaatctaatg tcttattttg tgtgacaatc gatccattaa tggttgaaat
    29821 tgaattataa ttcgcaataa aatcttctaa accatcacgt agttctgtaa tgtcattaac
    29881 tgtcgtagtg cgttcaatta atgccattaa cttgtttatt tccttatcaa tgtcagccga
    29941 ttccttatta atgtacttca tcacttcttt acgtaacttt cgttgctcat tttcagataa
    30001 agctactttt gtgataaata attttttatg tgttaggaca aacattggtg aaaagacgat
    30061 gtcataatct aatgtgtaat tttcaaatgt tctaagtgaa atcgcatcta agaaaataat
    30121 ttctggaaat aagtttcgca actcgtataa catcatttgt gatactgacg tgccttgtgt
    30181 acacacgata atagctttta tcttgccatc gaagttttca tcttgacgtc tcaaactacc
    30241 tccgaacaac atggttaaat atgctatttc attatcaggc aacgattttc cgaaatattc
    30301 agttaacgat tgacatgatt gtttcaccat atgaaataag gattgataat ttccttgtaa
    30361 aggatttatt aattcatcac gatccgttaa gttatattta atcctataaa aagcaggcgt
    30421 taaatgtaac aagagttgct gtgataattt ctccttatct tcaatgttaa taaaagtgat
    30481 ttgttcaaaa tggtgaatca tttgagcgat agccatcgtt aaattcgata tgttatctga
    30541 ttcctgcaaa tcagtccatt gcacacttgt tgaaagtaag tgtaatgtca aatataactt
    30601 ttccgcttct ggcaaatccg gctcaagttg cgtcataatc tccgttgctt gatattcttt
    30661 cgtatccctc aaatactgat aattaatatt taatggattc atcacatgac cactttgaat
    30721 tcgtctacga atcacacaaa ggacataagg caatgaacta agtgatttgt ctataaagcg
    30781 actcttcaaa aattgttcta cctgtttgat tttgtctttt tgatatgcga tatcttcgaa
    30841 tgttaagttg agcgccttta aaacttcact tttagtaata tcatgattca acctttgatc
    30901 aatcaactta atgaagaaac ggcgaacttc aaattcatca ccaacaattt cataaccatg
    30961 ttttcgagaa tacttaagtg acaaaccatg attttccaat tgctctttca catgatttat
    31021 atcgtgaatg acagtatttt tactgacttg taaatcaatt gaaaaatggt ttagagacat
    31081 tgcgttttcc ttactaaaaa gcatgagcat taaataataa cgacgtgttt ctatgctaaa
    31141 aatgacattg ttgccgttta acatttgctg ctctgataca tctcgtttga ataacgtcat
    31201 gatttcagaa cttacaataa aatttccttg gcttgttctt tcaagttttg gataaccctc
    31261 ttgttcaagc cacaaattga ttttttgaat gcgatatcct agttgtctac gagacaaacc
    31321 aaatatcgat tcaagttctt taccatgaat agtaggattc aatacaattt ctctgagtat
    31381 ttgctgatta tccccgttca tgttcgatcc tcctttgagc atctgagctc aatttatcat
    31441 caatcgtcac accgatgtac acactttgaa cacatatttt caaaatgagc atgtacatca
    31501 ttgtgatgtt ttaacaacat ttcaattata tctatatttt ttgtgatttt aatcttttaa
    31561 aataaagcaa ttgaaatttt gaatatattt ttgtattttg tgttttttga agcattttta
    31621 acatacatat ctcaatcatt atcaaattgt catgaccatt gtaatccaat acaaaaaccc
    31681 taaggacgct tatatcaggc accttagggc taactgtatc tatttaatta agtattatta
    31741 ttcgtatgta cgtaacttat ggtctatcaa gttccacact tcttcaacat caactgctgt
    31801 agcaaaataa gcattggcag gcttacctgt aacatgattt aaatcgacag ccatagtgcc
    31861 ataagttagt ggactttgat gttcaatgtc gatattaacg ggtaccattg taaacaattc
    31921 tggttgtaac aaatacaaaa ttgtacaagc atcatgtatt ggaccaccat ccatattaaa
    31981 gtgagtcttg tatgtcttct taaagaattg caataattct acgacgaact gtgcaacagg
    32041 attattgata ctttcaaagc gttcaatcac gtgatcgtcg gctaaaactt gatgtgttac
    32101 atctaaacca aacacattta tagtaatccc actttcaaaa acacgcttcg ctgcttcagc
    32161 atctacccaa atattgaatt ctgctgtagg cgtccaattt ccaaatgtac caccacccat
    32221 caaagtaata gattcaatat gctcagcgat tcttggctca cgaatcaatg ccgttgctac
    32281 attcgtaaga ggacctgtcg ctacaattgt tacaggtgta tcactcgtca tcactttgtt
    32341 tataatcaca tctgatgctg gcattgcaac tgcttgacgt gatggtgtcg acggtagttt
    32401 cggaccatct aatccagatt ccccatgtat ttcagaagca aaggcagctg gtttaattaa
    32461 cggcctatcc gcacctttcg ctactgctat atcttggcgt cccataatat ccaatacgtt
    32521 caaggcgttt gtcgtattct tgtcaactga ttgattacct gcgactgttg ttacagctaa
    32581 tatctctagt ggactgtcaa ttgcccccgc taaaattaat gctattgcat catcgtgtcc
    32641 tggatcacaa tccataataa tctttctttt catttatata tccacctttc ttaagttgtt
    32701 atcgatagct tatgtatatt tatttatgtg gtgaatcatg tttattttga aaaatagttt
    32761 taactttctc atatttttgg atacaaacac tatttatcta ttttatggct tataaattta
    32821 tccgatatgc cttatcaacc tacctcgcta aaaataggat gtctacatat ctataccgac
    32881 ttttgtcaac tcattttcac aacaatataa acagcaattt atatgattgt tacatgattc
    32941 aaacaatttt tatgaaaaat attttcatac acagaatata tattgatatt aaatttctca
    33001 aaagctatat tgagaataat taggagggat gttgatgaaa tctttatttg aaaaagcaca
    33061 gcagttcggc aagtccttta tgttacctat cgcaatctta ccagctgcag gtctattgtt
    33121 gggtatcggt ggtgcattaa gtaatccaaa caccgttaaa gcatacccta ttttagatat
    33181 taccttatta caaaatattt ttacattaat gtcagctgca ggtagtattg ttttccaaaa
    33241 tttaccggtc atctttgcaa ttggtgtcgc aatcggatta tctagaagcg ataaaggtac
    33301 tgcaggttta gctgcgctgc tcggtttctt aattatgaac gcaactatga atggcttatt
    33361 aactatcacg ggcacattgg caaaagatca gcttgcacaa aatggacaag gcatggtgct
    33421 cggtatacaa acggttgaaa ccggtgtttt tggcgggatt atcacaggta ttatgaccgc
    33481 aatacttcac aacaaatatc acaaagtggt attaccaccg tatttaggtt tctttggtgg
    33541 ctctagattt gtccctattg tcacagcatt tgccgcaatc tttttaggta tattgatgtt
    33601 tttcatttgg ccaagcatac aagccggcat ttatcatgtt ggtggatttg taacgaaaac
    33661 aggtgccatc ggtacttttg tttatggctt catcttaaga ttgttaggtc cactcggttt
    33721 acaccatatt ttttacttac cgttttggca gacggcactt ggtggtactt tagaagtcaa
    33781 agggcactta gttcaaggta cgcagaacat cttctttgct caacttggtg atccagatgt
    33841 gacgaagtat tattcaggtg tgtcacgctt tatgtcaggc cgttttatta cgatgatgtt
    33901 cggcttatgt ggtgccgcac ttgcaattta tcacacagct aaacctgaac ataaaaaagt
    33961 tgtcggcggt ttaatgttat ccgctgcact cacttcattt ttaacaggta ttaccgaacc
    34021 tttagagttt agtttcttgt ttgtcgcacc tattctttat gtaatccatg ccttctttga
    34081 tggattagca tttatgatgg cagacatttt caacattaca attggtcaaa ccttcagtgg
    34141 aggctttatc gatttcttac tctttggtgt gctacaaggt aatagtaaaa caaactacct
    34201 atacgtcata cctattggaa ttgtgtggtt ctgtttgtat tacatcgttt tcagattctt
    34261 aattacgaaa tttaatttca aaacacctgg tcgagaagat aaagctgcag cacaacaagt
    34321 tgaggctact gaaagagcac aaactattgt tgctggtttg ggaggcaaag ataacattga
    34381 aatcgttgac tgttgtgcaa cgagactacg cgtcacactt catcaaaatg acaaagtcga
    34441 taaagtatta ctcgaaagta ctggtgccaa aggtgtaatc cagcaaggca ctggtgtgca
    34501 agtaatttat gggcctcacg ttacagttat caaaaatgaa attgaagaat tgctcgggga
    34561 ttaagactaa ccgaaatatc aacagaacta atggcaacga tgtacgaagt aagaagtgac
    34621 atcgttgctt ttatttttaa tgttacattt gaagcattaa gttcatcatg cactgtagtg
    34681 agcccgcaaa tcgcctctgc tagacaatca tcttaatgct atgattaaag cttaagtgcc
    34741 agatttgaat ttaatttcaa caacgacttt cactacatta aaaatagggc tactcgacac
    34801 atatagttgt atcaaatagc cctttataca attttttggg taaggtttta caatttttgg
    34861 aatggtatag attttataaa aagttattta agttcttctg cttcagccat aatatctttt
    34921 aatgttttag ctgaatgtgc gaacttgctt tgttcttcgt cgtttaatgg gatttctacg
    34981 acgttgcgaa taccgtttct attgatgact gctggaacac caatataaac atcttcttca
    35041 tcatattcgc cttctaataa tgctgataca gtcaatacgg catcttcatt tctgaaaatc
    35101 gcttcagtaa ttctagctaa tcccattgca acaccataat aagtggcacc tttagcttga
    35161 ataatgtcat atgctgcatc acgtgtttga acaaaaattt gttcaatttg cgctttgccc
    35221 tcaggacgtt gttcaagtaa tgtcttcaaa ggttgacccg caatattagc gtgtgaccat
    35281 actggtaatt cagtgtcacc atgttcacca ataatttgag catcgacgct acgtggcgca
    35341 acatcgaacg cttcgcttaa caataatcta aagcgtgcag agtctaaaat tgtaccagaa
    35401 cctataacac gttctttagg taaaccagag aatttccatg ttgcatacgc taaaatatca
    35461 acaggatttg tagctaccaa gaaaatacca tcaaattttg atgccattac ttcaccaaca
    35521 attgatttga atattttcaa gtttttagat actaaatcta aacgtgtttc tccaggtttt
    35581 tgtgcagcac cagcacagat gacaactaga tccgcatcat gacaatcact gtattcgcca
    35641 gctttcacac gaactgttgt tggagaatat ggtgtggcat gttttaaatc cataacatct
    35701 cctcgaactt tttcagtgtc taaatcaatg atgactaatt catcaacaat gctttggttc
    35761 actaatgaaa atgcgtagct tgaacctact gcaccattac ctattaatac aactttgttc
    35821 cctttaaatt tgttcattac aaaaactccc ttatgattaa ttcactaaca tacgtgtagt
    35881 ttcaaatatg ttagtttaat gctgcttatt gacgatacaa aaacaaataa acatctcttt
    35941 tattttcaac gcataactta aaaggtcatg tgtcatccgc ttttaagttt gtgatttatt
    36001 tcacatataa aatgtaacat acattaagta ctaggtcaat attaaattgt gatttatttc
    36061 acattttatt ttaattttta caccttttta atttgtatac gattacatct tagatgtctt
    36121 tagtcttcgt acttcgccag tgattattta cactttcaca tttttattat catgtttact
    36181 tttttctaag aaaacaacaa tgttttttga attagtcaaa taaatgcgcg caatcgtcgg
    36241 tgtgcaaaca gacaattgta cacaatgctt attgataagt atttaaaaaa ttaaaaatgt
    36301 catacaatta tcaaatttgc cattttattt atattttctc aaactaatta attgaatatc
    36361 gaaattttta gtagaataat caaaatagac agattaaagg aggagtatca tgcttacaga
    36421 acaagagaaa gacattatca aacaaacagt gcctttactt aaagagaaag ggacagaaat
    36481 tacgtcaatc ttttatccaa aaatgtttaa agcgcatcct gaacttttaa acatgtttaa
    36541 tcaaacgaac caaaaacgag gcatgcaatc ttcagcatta gcacaagctg taatggccgc
    36601 agcggttaat atcgataact taagtgttat taaaccagtc attatgccag tcgcatataa
    36661 acactgcgca ctacaagttt atgctgaaca ttatccaatt gtgggggaaa atttattaaa
    36721 agccattcaa gacgtgacag gattagaaga aaatgaccct gtcattcaag cttgggcaaa
    36781 agcatatggc gttattgcgg atgtgttcat ccaaattgaa aaagaaattt atgatcaaat
    36841 gatgtggata ggttttaaac cattcaaaat taccaatatt aaacaagaat ctgaagacat
    36901 taaatcattt acagttgaaa ctgaagaata tgactttagt gaatttacac caggccaata
    36961 catcacagtt gatgtttcta gtgaaaaact tccatataga gctaaacgtc actattctat
    37021 cgtatcaggt gaaaaaaacc atttaacttt tggcgttaaa cgtgatgtca caacagaaca
    37081 tgaaggcgaa gtttcaacaa ttttacacga tgagattaaa gaaggcgata tgattaattt
    37141 agctgcgcct gtaggtggat tcgtattaga gaatacgact gaaccacaac ttttcttagg
    37201 ttcaggtatt ggtgttacac ctttagtagc tatgtatgaa gctgcctctg ccaaaggttt
    37261 agatacacag atggttcaag ttgctgaaaa tgaacaacat ttacctttca aagacaactt
    37321 caacagtatc gcaagccatc atgacaacgc taaattatat acacacttaa aagataaaca
    37381 aggctatatt ggcgctgaag aattacaagc atttttagca aataaacctg aaatttatat
    37441 ctgtggtggt acaaaattct tacaatctat gatcgaagca cttaaatctt taaattacga
    37501 tatggatcgc gtacactacg aaacatttat tccaagacta agcgttgcag tataactgag
    37561 aaaagaggtt tagtgctcat atgaaactta atcaacgtta cgtaaaagta tttgcattat
    37621 atttcgtaag tattgttact gcaaatatta ttgttaaaaa taataattta attaaaacat
    37681 tgatacaaac catagccggg tacacggtct ttgcagttgg tttaaagtat ttaactaaac
    37741 gtaaaaataa ataacatata tacaaaaaca gctatgactt tactttgtca tggcagtttt
    37801 ttaattggga gtgggacagt aatgagattt tcgcaaaatt tatttcgtcg tcccaccaca
    37861 acatgcattg atgtatgctc actgaatttc ataagaaagg agttcacaag atgaccgtag
    37921 atattggacg gatttatgac aataaagata ataccgacgc tattcgtatc ctagtcgata
    37981 gagtctggcc gagaggtatt tcgaaaagaa ctgctaacct agattattgg ttaaaagaca
    38041 ttgccccttc tactgagttg cgacaatggt tccaacatga tcctaaactt tttggagctt
    38101 ttaaagaaaa atatgaaaaa gaattacgtg atcaggatgc acaaaaagat gcttttgaaa
    38161 aattaaagga tattgtaaat cagcataatc atgttctatt gttatatgca gcaaaagata
    38221 ctaaacataa ccaagctgta gtactacagc agttgctcaa tacttaggta tatatgttta
    38281 tcgtgaatca ctataaatat cgctaacttc atttctaata tttattttta aaaagcataa
    38341 ttactacaat taattgaact ttaatgatta ctaaacttga acaatatttt actttaacaa
    38401 aataaagttt taaaatatta attgttgggt tttacatagt aattagaaag agagtgttaa
    38461 tgaaatgaag tttaaaagac tagcaactat attttcagca gtattagtgt tatcaggatg
    38521 tggctcaatg cattcatctg gcaaagactt aaatatttcg ttaccgttaa aaacaaagtc
    38581 tattgcacct tatgaaacag atgtaccagt taagattggt gctgcagaat cattatttaa
    38641 aacaaatgat caaggaaaaa tagaaaaagc attagtgaaa tcgtatcatc aaccaaatga
    38701 tacaacgtta gatatagaat taaaagataa tattaaattt caaaacggtc aaaaattgac
    38761 tgcagaaaaa gtgaaatcta gccttgaaaa tagcatgaaa aaaagcgact tggtcaaata
    38821 ttcattacca atatcatcaa ttaccgctaa aggtcaaaaa ctgacaatta aaaccaactc
    38881 cgcttaccct gaacttgtat ctgaattagc taatcctttt atggcaattt atgatacaga
    38941 tgctaaatca gatgttaatc aaactcctgt tggtacaggc ccttaccaaa taaaagatta
    39001 taagcaatct cgaaaaatat cattgtcgaa ttttaaggac tattggcaag gtaaacccaa
    39061 acttgatcat attactgtga cgtaccaaga agacggcaat aatcgcgtca gaaatttaga
    39121 atctcaaaaa gatgatttaa taactgatgt cccagttaat aaagttcaag acatagaaaa
    39181 taatcaaaat ttaaaagtgt caaaagaatc tggatttaga acttctttac ttatgtataa
    39241 tcatactaat aaaaaaatga ctaaatccgt tcgtgaagca ttagatcata tcattgatag
    39301 acaaggtatt gcagatcata tttatcaagg ttatgcgaaa cctgcaacga gtccatttaa
    39361 tgataaaatt ccatatatta aagaacctaa gttaaccaaa caaaatatcg aacaagcaaa
    39421 aacgttatta gctaaagatg gttatacaaa agaacaccct ttaaaaatta agttaattac
    39481 gtatgacggt cgtccagagc tatcgaaaat tgcgcaagta ttacaatcag atgctaaaaa
    39541 agcaaatatt gaaatcgaca ttaaaagtgt tgatgacata gaaggttact taaaagaccg
    39601 ttctgcatgg gatgcaacga tgtatagttt tgggacaatc cctcgcggtg atacaggtta
    39661 tttctttaat caagcatata aaaaagatgg tgctattaat aaaggagact acaataatag
    39721 caacgtcgat gatttaatta atcaattgaa tcatactgta gatgttaagg aacgtcacaa
    39781 tatttctaat gatattatta aattatcaag tcgagatgta ccaaacagtt atattgctta
    39841 caacgatcaa atagtcgctg ctaacgctaa ggttaaaaat tataaagtaa cacctgaagg
    39901 tatctattta atcgattatc gaacaacgat tgaacgataa gggagtgtgg gacattgatt
    39961 tatgtctcag ccctgcttca actataaaaa ccacgccgaa gtcatccaat atgattatgg
    40021 cgtggtttaa tttgcttata tgattgtatt aatttctgtt tgatgaattt caataaaatg
    40081 attattacat actttcagtg aatcatgttc attttatata aatctaattg tcactatttg
    40141 tttaacgtga aacacttttt taactccgaa aagtattcct attccactct tttaaagaca
    40201 aattataaaa atagcttatg tcacaaacat atgagcaaca taagctttaa ttattattcg
    40261 tctacttttt tctgttgttc tttaaattct ttctgcagta aattcacttg ttttaaacct
    40321 gcccccatta aaatgaatac aagtatccac acaataacga ttaaaataaa tatttttaac
    40381 gtatcactgc caagtacagt taaagatgta tcccaaatgc catgtaaacc aacggctgat
    40441 aaaaagaata ttaaaaagcg tttatcaaaa atatctttga attcaaagcc atgctgttct
    40501 ttcgcaataa ctattgcagc accaacaatc gctgaccaaa ctaaatgacc accaatcgca
    40561 gtccacgcac gtgtgaagac aatatctaat aatgggacat tttcccctaa agcgaaattc
    40621 aaaatataac ctgctgattc aaaaactgcg aaccctgcac caatagcagc accgattaat
    40681 aatccattca aaatcttatt tgttttcaat ttattgacga aataaacaat aatgagtgct
    40741 tttccagttt cttcaactaa tcctactaaa aatgcatcga aaaatgttaa agaaccaaac
    40801 ctttcgaatt gatcactaaa aacgacaaat ctatataata acatcgtact tagtaatgaa
    40861 aatacgccgc caataaagaa catgataata acttcaaaaa tgctaatatt tttaaacgca
    40921 tttgattcat aaaagaagaa caaacccgat aatggtactg ttaaagcccc tataaaaatg
    40981 agacccggaa tcgcgttagt gttattaaaa atcgctgcca tgacccataa tccaataaat
    41041 gttactgtga aagcgatgaa tactcgagaa aagagccatg gcttccccca ttcttctgat
    41101 atgtcacaaa ttgctggcgt agttttagca gtacccgcta taaatatttc atctgcttcg
    41161 tttttagtat gcggcttaaa tacttcagaa aacatatctt ttaaattaag atttaagctt
    41221 tcatcattgc cggctagtct accgattgat ttggttgcat tatcaaataa acttcttgct
    41281 tcggttttta aatctgcatt ttgcgctgtt tcatgcgctt tggcttccat ttgtttattg
    41341 atagattcat tatgtatgtc ggacgtctta gttgattgtg tacttgctga tgacatcccc
    41401 tcttcacctg tattaggtgc aactgattgc gtttgtgttg tctcttcttt ttctgtattt
    41461 cttcgatgat taagaccttc catagactta cctactttca cttttcataa taaaactatt
    41521 ttaatttaat attgatggat tttccatatt ttaatataaa tactttgttc gattgggcgc
    41581 tttgtatttc atatttaaaa atcaaaacct atttaacttt ttatattccg agcaatattt
    41641 ttatataagt attttaatga tattcaattc attacaaacc cttgatatta caattgttat
    41701 caaccatcaa aatgtgtcta tactgtgtct attatcagaa taaaaagata tttctaattg
    41761 attttaaacg tcgttatgtt atatttcttg taagggttta cacaaagtgt aaaaacgcta
    41821 tattttatca aatgtgatgg aggtgacttg cttgaaacaa atcacatggc acgacttaca
    41881 acatatcatt aaagatggtg atgtgattgg tttaccagca ttagctgtag ccaacttacc
    41941 cgccgaagtt ctacgtgctg tgttagcgca acatgacaca tatcatacgc ccaaagattt
    42001 aacgtttata ttagcgaatg atatccatag tttaggtgcc gcaccggatt tagatgattt
    42061 tatagaacgt cgcatgatta aacgtgtcat tatgagcatt ttaacggctt cttccaaaac
    42121 ggcacaagca atgaaaaata atgacattga agcttatttt ttaccacaag gtatcattgc
    42181 aactcattat cgtcagagta atcaattatt acctggagtt attactaaaa tcggattaaa
    42241 cacagctgtt gatcctagat acggtggcgg taaagtaaat acacgaacaa ctgatgattt
    42301 agtttcatta gtaaccatca acgatgaaac atacttacat tacacattcc ctagcgttga
    42361 tgtggcacta ctgagaggaa catacgcaga tcaacaaggt aacatttatt taactcaaga
    42421 agcgtacttg agcgagtgtt atcatgtcgc attaaacgcg aaagccaatc atgggaaagt
    42481 tattgtacaa gttaaagctt tagttgacgg atatcaacta aaaccgaatg aagttgttat
    42541 cccaggaaat cttgtcgatt atgtatacgt cacagaagat gaaaagaatc accgccaagt
    42601 aattcagagt cattatttac cagccttgtc tggagaagaa cgaattgatg gaatacctga
    42661 acccgcatta ccttttaata gtcgcaaatt gattctccga cgtgctgctc agtttttaac
    42721 ttatggcgat acaattagca tcggttatgg catcaataat gaactctcta atttattgca
    42781 cgaagaatgt gttgaacatg atgtgcaacc gattttagat gttggcattt tcggtggatt
    42841 cgttgggagt cgtgaacatt ttggtatgaa ttacaatgca gatgtgcgca tgcctcatga
    42901 tcgagcatgg gattttattt ataacaatgg tgtatcagtt gcctatctta gctttgctga
    42961 ggttgatcaa tacggcaatg tcaacgtgtc ttacttcaat gaccgactaa atggatgtgg
    43021 tggctttata gacattacgc aatctgtaaa taaaattatc ttttcaggta cttttgtagc
    43081 tggcagtcat gtctcatgcc ataatcaacg attaaacatt gaaactgaag gacaaaacca
    43141 gaaatttgta tcagatgtga gccatatcga ctttaatgca caatattcac aatcactcga
    43201 gcaagaagtc tattttgtta ctgagcgtgc agtattcgaa ctcaccaatc aaggcttgaa
    43261 actaattgaa attgcaccag gtcttgattt gcataaagat attttgaatc aaatggcttt
    43321 taaaccaatt attgctgatc atttaaaatt aattgatacc agcatttaca aagaaaaatg
    43381 gggacaactt aaacaatcaa ttcataaagt atgaaagtga agctaaaggg ggtatactaa
    43441 tgaatttcga ttggataaaa acgcgttcag acttcgatga tgacaagcct gccgttattg
    43501 atcacgcaaa acaaacatct tggacatacc aacaactcaa tgcacgcgct gataatatgg
    43561 cacattattt aacatctcaa ggtgttaaaa aaggcgatgt tatcggtatt tttgcgccaa
    43621 atgatattgc aatattagat ttattgtttg cttgttttaa aacaggtgcg gtttttttac
    43681 cattgaattg gcggcttaac ccaaaagaaa ttgcagccat tgtggaagat gcacaattaa
    43741 aactgctctt ctatgctgaa aaacatttaa gttcactcac cgatattgac caaaacttat
    43801 tgcatatgga tattgatgta gcgcaatatg atgaaatcgt aaatccagat tatcaccaac
    43861 cttttcaagc aacacctgtc gaaccacaag atcttgcagc attaatttat acaagtggta
    43921 caaccggatc acctaaaggt gtgatgttct catatgaatc atttgttcat aacggtgcaa
    43981 acctagagct gacgtataag ttcaattcaa actatattac gattgtatca acaccaatgt
    44041 tccatgtttt agggtttaac gatactgtat taccagtatt gatgtcaggt ggcacactta
    44101 ttcttcaacg ttactttaac ggcgaagaat tgaatgacat gattgcacaa tatcacccta
    44161 catttattat catgattccg acaatgtatt atagtacgct acgtgctagt aattttaatc
    44221 ctgaaaattt taaagctatg gattatatca tccaaggtgg ttcacaacca ttaccaagta
    44281 tccaagcagc ttttaaacaa tatggcataa acattattaa cggctatggt ttaactgaag
    44341 cacctcttgt acttgttaat acaccggaaa attcaaaacg taagccaatg agtattggta
    44401 aagcagtcat gttcgttgat gcacgtatcc ttgatgataa cggtgaggaa gtacctactg
    44461 gtgagattgg cgaacttgca attaaggcta aaaatgtcac gccaggatat tggaataaac
    44521 cagcagagac tgccaaagca tttcatggtc gttatttatt aactggtgac ttagcgaaga
    44581 tggacgacga tggcgatata tttattattg aacgcaaaaa agaattaatc ataactggtg
    44641 gcgaaaatgt cttaccatcc gaagtcgaaa atgctttagc tgagcatcca ctagtagacc
    44701 ggtgtgtggt cgttggctat gatcatccaa aatatggtga atcaattgct gcagccatta
    44761 tacttcgcga agatgaacct cattacgctg aaattttaga tcaacatatg cgaagtcgtt
    44821 tagcaggtta taaagtccca agaatgtatg taccagtgac acatatgccg ttaaacagta
    44881 cgcagaaacc agataaactt gcgattcgac aaatgatgaa tgacaaagtc tcacaaacac
    44941 tttaaaggtg ataaaaattt ttgacattta gtgtaagcgt ttacaaataa agcgtgttgt
    45001 ttttgaatta aatgcatttc acattagtat tcatattatt tttaggagga atttatatga
    45061 catttgaaaa agaaacggtc ttaaaaacat tatttcctga agatgtactt agtattgcta
    45121 aaggtttaac agacggtgaa gtcgaatttt tacaacaagt agattcattg ctagaaagta
    45181 agtaccgtga aaatattaat caacattgga tagacgctac tgtacccgag gactatttta
    45241 aagatctggg agaattaaat tattttaaca atccattact ttacaaggat cgtccaaacg
    45301 ccaaaatgcc tagtcaacta tttcagtttt tcatgtctta cctactcgcg cgatttgata
    45361 tttccttagc taccctactc ggtgttcacc aaggtttagg gcataacact ttctatttcg
    45421 gaggtagcaa agaacaaatt gcgaaatatg tacctaaatt acagtcacat gaactgcgta
    45481 catgctttgc tttaactgaa ccagaacacg gttcggacgt tgcgggaggt cttgaaacag
    45541 tcgctgaacg ccagggcgat acttgggtta tcaatggtga aaagaaatgg attggtggtg
    45601 cacatgtatc tgatgtcatt ccagtattcg cagtaaataa agaaactggc aaaccccatt
    45661 gctttgtagt cagaccagaa caagatggcg tcgatattga agtcattgat aataaaatcg
    45721 cacttcgcat tgttcctaac gccctaatta aattaactaa tgtcaaagta gatgaagcgg
    45781 atcgcttaca aaacataaca agctttaaag atattgccaa aattctttat tcaacgagag
    45841 caggcgttgc ttatatggct acaggtggta tggctggcgc tttacgtgcc acattagatt
    45901 atgtcactga gcgtaagcaa ttcggcaaac caattagtaa atatcagtta atacaagaaa
    45961 agctagcaat gatgcaaggt aatttagctc aagcaatggc aacatgtgct caattagcta
    46021 atatgcaagc acatggtgaa tatgacgagg ttgcaacttc aacggcgaag atgatgaatg
    46081 ccttacgttt gcgtgagaca gtagctatgg gccgtggtat tacaggtggt aatggcatac
    46141 tagctgacga ttatgatatt gcacgtttct tctctgatgc agaagcgatt tacacgtacg
    46201 aaggtacaca tgaaattaat gccttagtaa ttggacgcgc tttgactgga gattctgctt
    46261 tcgtataaat agcaaagaat tatatgagat gcagtaatat cactaaaaaa agacttattt
    46321 taagcataaa gctttttcct taaataagag gctaagatga ctgtcaaaga tacttaatta
    46381 attttataaa atagcaacgt tattccaatt atcttaatgg ttatcttatc ctcaactaaa
    46441 ttggaggaat cactatgaca attaataaag taaccgttct tggcgcaggc acgatgggcg
    46501 ctcaactggc agcactcttt gtgaatgctg gacttaaagt aaaactatta gatattgtag
    46561 tggacaaaaa cgatccaaat ctcattgcga aaaaatctta cgataaaatt acagataaga
    46621 aacggccgct actattcgac ttaaatctag cgagtcattt aacatatggt aattttgatg
    46681 atgacttggt aaatgatgat gctgatttat atatcgaagc agttaaagaa gatattgaaa
    46741 ttaagcatgc tgtttggcaa caagttctac aacatgctaa agaagatgct ttattcgcta
    46801 caaatacatc aggtattcca attaatgcga ttgctcaaac atttaacgag aaggatcaag
    46861 aacgattctt tggtctacat ttctttaacc caccacgtat tatgaaatta gtggagttaa
    46921 tacctacgtc acacacgaag gaatctatca tattagatgt aaaaaatttc gcgcaaaatg
    46981 tgttaggtaa aggtgtcatt gtcgtcaatg atgtgcctgg ctttgtcgca aatagagtcg
    47041 gcacgcaaac aatgaatgat attatgtatc gcgccgagca acacaagtta agcattgtag
    47101 atgtggatgc tttaactggg caagcgattg gtcgtcctaa aacaggtaca tatgccctat
    47161 ctgacctagt cggtttagat attgcagtgt ctgtaattaa aggcatgcaa caagtacctg
    47221 aagaaacgcc ttattttcat gatgtcaaaa ttgtaaatac gttgtttgac aatggcgcac
    47281 tcggacgtaa aacgaaacaa ggattttaca aaaaggataa agaaactaaa gctcgacttg
    47341 tttacgatgt tgaaaaacaa gattatgtac ctgtatcgca accacaatta ccaattttaa
    47401 atgaatttaa taaagactta gtgcataacc ttgatgtcat attcaatgcg caagacgaag
    47461 cgggactatt tttatgggag acattacgta ataatttcta ttactctgct atcaatgtac
    47521 ctaaagctac cgatgatttc cgagacatag accgtgcgct tgtctggggg ttcaactgga
    47581 aacttggtcc attccaatta tgggatgcaa tgggatatga acgtgttaaa acacgtatgg
    47641 aagacgaaat tggagactta ccacaatgga ttagtgattt agatggtggc ttttataaac
    47701 aagatgagac catcgaatat gcaacacctg tttctcactt cgtaaaagat gaactttggg
    47761 ataaaggtga tgccaaactt tccgtaactc atgatgatca actgttactg aaattacaaa
    47821 gtaaaaataa tgtcattacc gatgaattca acgatgcgtt agttgatgcg attgatttac
    47881 tggaaaatga ccattacaca agtatggtta tttatgcaga tggtaacaat ttcagtgtgg
    47941 gtgctaacct tttcttaatg aaaaaggcgc atgaagacgg tcttgtagat gatgtcgttg
    48001 cacaatcaat tgataaatta cattatagct ttaatcgttt gaagtatagt ttgaaaccag
    48061 tagtcacagc tgttcaaggt cgtgccttag gcggtggctg tgagcttgta ctttactcac
    48121 ctattgttgt cgctgcaagt gaaacgtata tcggtcttgt tgaagcaggc gttggcttat
    48181 taccgagtgg cggtggcctt gcagaaatgg ctgatcgcat attacgcaca tcgcataagt
    48241 ttgatgacaa acaagcttcc atgacaaaag tactgacgaa tatcgcattt gcgaaagtct
    48301 ctacaaatgc ctttgaggca cgtcgttatg gttatttacg tgatacagat acgattattt
    48361 tcaatacagc acaacgtgtc gaagttgcgc tcaaacgtgc gaaatatgaa gcagaaacaa
    48421 actatattcc gaatcctaga catcaatata tcgctttagg tgaagacttc aaagcattga
    48481 tccaaggaca attagatgcg caaagacggg gtcattttat tagcgaccat gattatcata
    48541 tcgccttaaa tatcgccaca attttagcgg gtggtgattt accaagaaat acatttatca
    48601 atcaacgtta cattcaatcg ttggagaaaa ttggctttat tgacttacta aaatctaaaa
    48661 aatcatatga aagaattgca catatgttaa aaactggtaa gccattacgt aattaaaaga
    48721 tagtcattaa gagaggatga taaccatgca agaagcatac attgtagctt atgggcgttc
    48781 agccgcagcg aaagcaaagc aaggcgcatt attccacgaa agacctgatg atgtcgcagc
    48841 caaagtatta caaggcgtat tgaaacgtat tgacggaaaa ttcaataaga atatgattga
    48901 agatgtcatt gttggtacgg cttttccaga aggattacaa ggacaaaaca ttgcacgaac
    48961 gattgcattg cttacgggat tatctgacac ggtaccgggt caaacagtga atcgctactg
    49021 ctcatcagga ttacaaacca tcgcgattgc agccaatcaa attatggctg gtcaaggaga
    49081 tatacttgta gctggtggcg ttgaattgat gagtgccgta ccaatgggtg gcaacgagcc
    49141 cacaaacaat cctaccttac aatatgatga tataggtgcg tcatatccta tgggtttaac
    49201 tgctgaaaat gtagcatccc aatttgacgt atcacgcgaa gatcaagatg cttatgctgt
    49261 cagaagtcat caacgtgcct atgaagcaca acgtgatggt cggttcaacg atgaaattat
    49321 cccaatacaa gtaaactcag ttgaatatac aaacgcagga ccaaaagtac acacaaatat
    49381 ctttgaccaa gatgaattta tacgccctga caccacgatg gaggcattag ccaaattacg
    49441 tacagtattt aaagctgacg gcactgtgac tgcgggaaca tctgccccac tttctgatgg
    49501 tgcaggattt gtagttttaa tgtctggaga taaagtgaaa gaactaggcg tgacacctat
    49561 tgcacgattc gttggttata aggcagtagg cgttgacccg aaaattatgg gtattgggcc
    49621 tgcatatgcg attcctgaag tattgtcact cagcaatcta tctgttgaag acattgattt
    49681 gatcgaattg aacgaagcat ttgcttctca aacaattgca tctattaaag aagtaggtct
    49741 agatatatca cgtacgaatg tgaatggtgg cgctattgct ttaggtcatc cattaggtgc
    49801 tacaggcgca atgttaaccg cgcgtttact taatgaaatg ggtagacgtc ccgatagccg
    49861 ttacggcatg gttacgatgt gtattggtgt cggtatgggt gcagctgcta tatttgaata
    49921 tgtgcgttag aatggttgat tttggatgaa gcggattcgt tttgttattg aatgaagtag
    49981 gctgaagttg aagccagttg aagttgaagc gggttgaagc aatttcgttt tattaatgaa
    50041 gctgtgtgaa atatagtgat tgaacaaaaa agtggtttaa tgggatggtg gttatttccg
    50101 ttttagaatt taacatttac acgtctaatt ttaatcattg ttttaaattt tatgaatcga
    50161 agccctttga tttaataata tttgctaatg ctagtaactt atctgattgt tcatgtttaa
    50221 aataaagaaa accactcaca tcagtgtgtg ttcgaactag acttgtaagt tccagttcgg
    50281 cacgactttc taaagcaatt attattgctg tgattgtcgt atatcactta gatgtgcgtg
    50341 gtttatttta ataggttagt aatatattag gtcatgttat gtttaagact ataatgaata
    50401 aataatttag aaatatgctt ccgattgttc gatgctttaa ttcagttaga agcatcatag
    50461 aatgcatgat tactgttgta aagatacgta atgttttgta ttgactgtat gtctttggat
    50521 agagttacaa acttattttg ttactctagg cccatatgtc gcagtaccat ctgcatgtgt
    50581 tgttacattg tatgcatttg ttttacttgg cttcttgtat gtcgggcgag ctccgtatga
    50641 cacttgaccg tttgcgtgtg ttgttacgtt atatgcattt gttttgcttg gcttgttttg
    50701 tgtcgggcga gctccgtatg atacttgacc atttgcatgt gttgttacgt tatatgcatt
    50761 tgttttgctt ggcttgtttt gtgtcgggcg agcgccatat gatacttgac gatttgcatg
    50821 tgttgttacg ttgtatgcat ttgttttgct tggtttgttt tgtgttgggc gagcgccata
    50881 tgttactgtg ccatcttgat ttgtcgttac gttgtatgcg tttgtttctg atggcttatt
    50941 gaatcttggt ctcgcttcat atccaaatgt tccatcgttg tattcacgga tacctgtacc
    51001 agcatctcta tatttaacat atttaggtgt tttgttaaat tgaggtcttg ctggataatg
    51061 tgatgcttct gttgtttcag atgcttgagg tttaacttca atatcacttg attctccttg
    51121 agtacctttt aacgttgatt cagtaccttg tggttttatt tcaagtttag atgagctacc
    51181 ttcaagacct tctaaaatag ggttcggtgt cgtcggttga gtataattat cgcttaaaga
    51241 tggtctgttt tgttccattg ttgggaaatc tggaccttga acgatttcac cttgtaacgt
    51301 tttattttcc atcgttggat attccggacc ttttacaatt tcacctgtaa ttgtgccctg
    51361 tggaatttta actaatggct gtgccactgg ttgtgttgtt tcttcagctt tatcaactgt
    51421 aattttaacc tcttgttggt tatcaaattt aggtgattga ggttcttcaa ctttcttctc
    51481 ttcttttaca actggagatt ttgttacagt ttcctcgtat tttttgacag ttttattttt
    51541 ccaagattcg tcagcctttg caaccgcttc tcttgtttct ttaactagag catcaaatcc
    51601 atctttatgt ttatggtaat catgtttagt tccatcatac ctagtaatgt gtttaggtct
    51661 attcaacttg gtttcaatga agaaatcatc aataatagat tctaaatctt taatcatttc
    51721 tttttcagta cgttgattcg taactctaat tggatctttt tcatcaccta aaatcaaatc
    51781 aattttcgcc ctaagattta atgcttctgt tttatgttgt gcatctgtaa cgtaggcaaa
    51841 atatacacta tatgcttgat caacaagtga atctatttta ccatatgctg ttctttcttc
    51901 gctttcagaa tatggtttta aatctggatt ctctgattca attttcttta cttctaactt
    51961 aaagttatcc aatgcttttg ttaataattt tgataagtca ttgtattctt ccatagttaa
    52021 atcgccaaga tcgtatttat caaatgcttt cattttaact tgagtattag gattctcttt
    52081 tttgtatttt ttatatagtt ctttataaat ttcgtatttt tcttttttct ttctagcagg
    52141 tattggtctt cctctaaaac tctagttttt aatttatcta ctgcatcttt atattctttt
    52201 tctgcatatt tatgctgatc cagtaaatga aaaatattgt aaaactggtt ttctagattt
    52261 tcaattattc cccaataata tccatctgca atttgtttcc catttttact cccagcatta
    52321 acttgtgatt tcccactata atcctttgtt actatcgcat ctgctttgtt atcccatgta
    52381 aataagctag atgcaactgc taatgcgcct agcgaaatta tttgcttttt cataattttt
    52441 taattcctcc aaaatgtaat tgcccaatct acattaaaga aacaaaatat taaaagacat
    52501 taactatata ttaactagaa taaacaaagc attaactatc tttttgttat agttaattag
    52561 ctttgcaata tacaatagtg tataattttt ttgattaatg tacatcaaag gagtaacaaa
    52621 gcatgacaac acaaatgaaa atcaaaacat atttagttgc tggtattaaa gcggcgctcc
    52681 ttgatacgac tggtattaaa ttagcaagca aatctgaaac tacatcacat acgtatcaac
    52741 atcaagcgct tgtagatcaa ttacatgaat taatagcaaa cactgactta aataaattat
    52801 cgtacctaaa tttagatgcg tttcaaaaac gcgatatttt agctgcgcac tatattgcaa
    52861 aatccgctat acgcactaaa aatttggatc aaatgactaa agcgaaacat agattagaaa
    52921 gtatttacga ttcaatttct aaccctttgc attcacaaaa caattagcaa cttatcaata
    52981 aatcatgtaa gtgtttgtga cgccaaattg ccatacaaat acttgtcata tgaatataaa
    53041 cgaatgaaac gattgcctat cccatagatg gcaacattaa aaagacctct gaaggtatca
    53101 tcttgataac tttagaggtc tttgtttata ttattcaaac aaaattctta tagaacgatc
    53161 gaagtatgtt tcgtctttct tttcttcttt aatctgatca gctaatgctg ggtcatctgt
    53221 gataatacca tcaacattgg tttgtaagta tttcgttaaa tcttcttcgc cgttaatggt
    53281 ccaagtatag acttctttat tttccaagtg cgcttgatta acaagtcttg gcgaataaga
    53341 aaaatcttcg atgacaaaga aatctaatga tgtttcttta aaatgaccaa actgcaacgg
    53401 aatgatataa ccacacttga gatatggcgc ttcttttttc aacttagtca tcacatcata
    53461 atccaatgac atcacacgat attgatgttc aacaccatgc tttttcaaaa tatcaataac
    53521 acgttgtgta taatctgctg gttctttacc atgtggcttt aactctacta gtagcttcac
    53581 atttgattgt ttagccgttt caataaattc gtctaaggat acaagttttg cttcatgtcc
    53641 attttgacgc attttcaaac cgacgacatc tttgaaatta gattcagaaa tatttttatt
    53701 aacacctgtt aaacgtttca aattgttatc atgactaaca acaaattgtt tatctttcgt
    53761 cataattgta tctaactcaa cgtattcgac attcgctttt gcagcagctt tcaatgacgg
    53821 aatagaattt tcaacgcctt tatcttcgaa accacgatgg ccaataatgg agatatttgt
    53881 attgatagta ttattgtaaa gtaagtacat gttataaccg ataaaacatg tcactgcaag
    53941 taccattgaa attataaaga acctagactt ccgtttcggt tttgggtatt taaattctaa
    54001 gcccggttgg tctaatacat tctcttgttt taagtgcagt actaacacac tgattaatga
    54061 taatttcgta aataaataat agaagaacaa tgcgcttttc aatacaacaa ataaaattga
    54121 tgagactaaa aacttatctc cttcttcatc tacacaaatg gcaagatatg ttgctcctga
    54181 aataattaat gttaaaatcg caccaatgat gagttccaat ataactattt ctataacaag
    54241 tcgaaactta tttcgcttcg taatttgcca acttagtctc atatttttaa ataacgactg
    54301 acggtttaaa atcgttaacg gtaaggtaaa tattaattta aagtttaata taaatacagc
    54361 aatcataaag gtaccgtaaa tgattatacc tttcgtcgtt ttcataagtt cttccgttaa
    54421 aaatttaggt atgtaaatat tttttgttaa tactgaactt agtcctaggt tggcaatggg
    54481 tatcattaac attaaataaa taacaaagaa aataactggt acacctatga gtttacgcac
    54541 atttacaaag gcatttttaa aaatggattt aaatgtaata atctgtcgat caaagccggc
    54601 ataaaccata taaactaaca atgaaaactc tacataaatc agaaaggcaa cacttaatat
    54661 gaatataata agaagtatca cactggcggg atgactaacg atttccgtcc aattgttaat
    54721 cgtaagttgg ctttgcccag ctacttttaa catcatatta aatagtaaaa ttaagtatgt
    54781 actactaata aaaatcatga ttaactgcaa tagtaaggca ttaatgctaa aacgcccttt
    54841 attttgatac agtaatttaa atactgccca tatatcttta ctaattctct tcataatcac
    54901 gctccgcatt gctttaatat taagtttcat cttaatattt ttcattactc agggtcaata
    54961 aaaatttgaa aagactcata ttcatatgca agtagcaaat aataacccat tcaacatcag
    55021 ctaaatgatg atattggaac cccaccttta acaagacatc acattcttta tcagcatagc
    55081 tacttacaaa aacgtcttcc ttcaaccatt gtagaagttg tctcatatac ttactacgtc
    55141 ttgctaatac tctatactca attgaaaaag aagcatatgc cccttcactc ttgaagttgc
    55201 atatgctttc ttttcggtct gaattgtatt tataattcaa cgggaatttt ccctttgaag
    55261 ttaacataac ggtaggctgc tttaacagct tcatcatcgg gcgcttcgac atcttctaat
    55321 tcatatgcaa tgcccaatgt tttccactta tgaacaccta actgatgata tggcagaatt
    55381 tcaaactttt cgacgttatc aagagaatta atgaattccc ctagtttaat taaatcgtct
    55441 ttatcatcag aataaccagg cacaaggaca tgtcgaatcc atacaggttg tttcatatct
    55501 gacagtttgc gcgcgaagtt aaggatgtgt gtattaggct ttcctgtcaa tctaatatgt
    55561 ttgtcattat caatatgttt tatatctaat aatatcaagt ctgtatgttt ttgtaattct
    55621 tcaaaatgcc tttgaaatgc ttttgtatca ttagcacatc cagccgatgt gtctaagcaa
    55681 gtgtgcacac cattttcttt taattccgca aataattttt ctaagaatgg catttgtaac
    55741 aatggttcgc caccactgac tgttacaccg ccacccgatg catcaaagta tggtttgtat
    55801 ggtaatattt cattcaccat ttcatcaact gtgacttctc ttgatggctc actaattttc
    55861 caagtatctg gattgtggca atacaagcat ctaagtaagc atccttgtgt aaataatata
    55921 tatcttaatc ccggtccatc gacagtacct aaactttcga cagaatgtaa gtgtccctta
    55981 agcatagtgc tcccacctta aattttgtta catactttca tggaatgtac gagaaattac
    56041 atctaattgt tgttcacgtg ttaatttaat gaagttaaca gcgtaaccag atacacggat
    56101 tgttaactgt ggatattctt ctggatgttc cattgcatct attaatgttt cacggttaaa
    56161 tacgttaata tttaagtggt gaccacattg cattgcgtaa ccatctaaca tactagttaa
    56221 gttacggttt tgatcttctg gttctttacc taatgatttt ggtacgatac tgaatgtatt
    56281 tgaaatacca tctttacagc aatcgtaagg gatcttagct acagaactta atgaagataa
    56341 tgcacctttt tggtcacggc catgcattgg gtttgcacct ggtgcaaatg gttcgccagc
    56401 tttacgtccg tctggtgtgt taccagtttt cttaccgtat acaacgtttg aagtaattgt
    56461 taatacactc attgtatgtt ctgaatcacg atatgtttta tgactacgta atttagtcat
    56521 aaagcgttct actaaatcaa ctgcgatatc atctacacgg tcgtcattgt taccgtattt
    56581 agggaagtcg ccttcgattt caaagtctac tacaagacct tcttcgttac gaattggttt
    56641 aacttgtgca tatttaattg cagataatga gtcagctgct actgataaac cagcgatacc
    56701 tgttgccatt gtacgtacaa tttctgtatc atgtaatgcc atttcaatac gttcatagct
    56761 gtatttatcg tgcatgtagt gaataacatt taatgagtta atgtaaacac ctgctagcca
    56821 atccatcatt tgatcaaatt tcttgaatac ttcgtcatat tctaatactt cgctgttaat
    56881 accttcgaag tttggaccaa cttgtgcacc agatttttca tctttaccac cattgatagc
    56941 gtaaagtaat gttttagcta agttcgcacg tgcaccgaag aattgcattt gtttaccaat
    57001 tgtcatcgct gatacacaac atgcgatacc atagtcatcg ccatagcttt cacgcataat
    57061 gtcatcattt tcatattgga tagaacttgt tttaatactc atttttgcac agtatgtttt
    57121 gaagttgtca ggtaaacgta ctgaccataa tactgttaag tttggttctg gagctggacc
    57181 taagttatct aatgagtgta agaaacggaa tgagtttttc gtaacaagtg gacgtccgtc
    57241 aatacctaca ccaccgatag attcagttac ccaagttggg tctccagaga ataattcatt
    57301 gtaatcaggt gtacgagcaa atttaacaat acgtaatttc atgatgaagt ggtcaataat
    57361 ttcttgaact tcgctttcag taataacgcc tgctttaagg tcacgttcag catagatatc
    57421 taagaatgtt gatgtacgac ctaaactcat tgctgcaccg ttttgttctt taattgcagc
    57481 aaggtatgct aagtataacc attgaactgc ttctttgaag ttttctgctg gacggcttaa
    57541 atcgaaacca tatttttgtc caagttcttt taattctttt aatgcacgat attgttctga
    57601 taattcttca cgtaaacgaa ttacatcttc tgacatttct gtagacatcg tgttgaagtc
    57661 gtgcattttt tcttccatta agaaatctac accatataaa gctacacgac gatagtcacc
    57721 gataatacgt ccacgtccgt atgcatcagg taaaccagtg attacacctg ctttacggca
    57781 gttcaacatt tctctagaat atgcatcgaa tacaccttgg ttatgtgttt tacgataatc
    57841 tgtaaagatt ttttcagttt cttcgtctaa ttcgtaaccg taagcttcac aagctgcttt
    57901 cgccatacga ataccaccga atggttgcat tgaacgtttg aatggctttt cagtttgtac
    57961 acctacaatt gtttctaaat ctttgtctaa ataaccagca tcatgagatg tgattgttga
    58021 agctactttc gtgtccatat cccacatgcc gccacgttca cgttcttctt tcgataactg
    58081 cattacttgt tcccaaagtt tagaagttgc ttctgttggt cctgctaaaa atgaatcatt
    58141 accttcataa agagtgtagt ttaattggat aaactctctt acatctacgt gtctgttcca
    58201 tcttccattt ttaaatcctt gccaagctgt tgcatgattt ttatttgttt ctaacatact
    58261 aaacgcctcc atctgtataa tgttgtgaat ttgaaatgct atgttaacgt tttcatagtc
    58321 attgtagcat gtttgttgtg atttttttca cataaattat gtacaatttt catcaaatgt
    58381 atgtcaaaac atagaaactg tactaatttg tgtaacagta atatgctaat tgcattctgt
    58441 tacacaacag ttataatgtt cactattttc attgctttaa aagttaattt aggagtatat
    58501 atttgtttat aacaaatgat tgttgataag cgttaaatca acacttagaa agatttactt
    58561 ttatatatga agagactgga ttaaatactt ttattgacgt aaaaatatac ttttgaaccg
    58621 ttcaatatct tgccaatttt tatataacag ctacaaataa aatataacag tttgatttta
    58681 caacctcggt aagtcatctt gacaaacaaa aattatgtgc tatcacaaca tttgcaacgt
    58741 cttaacaagt catctttaaa catttctaaa tatttaacat tacttatgcg tcatttattg
    58801 ctaaaattat tgtattaaaa tatacataga attgatggga tatcatgaaa tcaaaaattt
    58861 atatcttgct attatttctc atttttttat cagcatgcgc taatacgcgt cactctgaat
    58921 cagataaaaa tgtattaaca gtttattctc cgtatcaatc aaacttgatt cgtccaattt
    58981 taaatgaatt tgaaaaacaa gagcatgtca aaattgaaat caaacatgga tctactcaag
    59041 tactgctttc aaacttgcat aacgaagatt tttcggagcg tggtgacgtc tttatgggtg
    59101 gtgtgttatc agaaacaatt gatcatccag aagattttgt tccctatcaa gatacatctg
    59161 taacacagca attagaggat tatcgctcga acaataaata tgttactagt tttctattaa
    59221 tgccaacagt tatagtagtg aattcagatt tacaaggaga tattaagatt cgaggttatc
    59281 aagatttatt acaacctata cttaaaggta aaattgcgta ctcaaatcca aatacaacaa
    59341 cgacaggcta tcaacatatg cgcgctattt atagcatgca tcatcgagta agtgatgtgc
    59401 atcaattcca aaaccatgcg atgcaactgt caaagacgtc taaagtcatt gaagatgttg
    59461 caaaaggtaa atattacgca ggtctaagct acgaacaaga tgcacgcaca tggaaaaaca
    59521 aaggttatcc tgtatcaatc gtttatccaa ttgaaggaac aatgttaaat gttgatggta
    59581 ttgctttagt taaaaatgca caccctcatc ctaagcgtaa aaagttagtg caatatttaa
    59641 caagccgctc agtacaacaa cgattagttg cagagttcga tgccaagtca attcgaaagg
    59701 atgttagtga acaaagcgat cagtctatcg aaaatcttaa gaacatacct ttaataccga
    59761 aatctaaatt accggacatt ccacatcata aatttttgga gatgattcaa tgacggcata
    59821 caaaccttat agacatcaat tgagacgatc gttatttgcg tctactattt ttccggtatt
    59881 tttagtcatt attattggac tagtaagctt ctatgccatc tatatttgga ttgagcatcg
    59941 tacgattcat caacatgttg atgaaagtca gtcatcttta catcacactg aaaagcagat
    60001 tcagacattt attacacagc ataataattc gtttcaagag ctcgatttaa caaatcatca
    60061 tgatgttacc gctacaaaac gtggattatt aaaattaata caccaacagc ccgcaacact
    60121 ttattatgaa ttatctggac caaatcagtt tataaccaat aattatgaac atttaaacac
    60181 taaaaatatg tatttatttt caacgcatca gcttaaattc aaaaatagta catatatgct
    60241 taaaatttat atggctaaca ccccacgttt aagtgaaatt aagaaagata gccgacaatt
    60301 cgcacttatc gttgatcaat atgacaatat tttatacgcc aacgatgacc gttttactat
    60361 aggtgaaaag tatagaccac aacaattcgg atttatgaat gaatctgtaa aattaaacca
    60421 tgctgaccat cgtctcatta tttataaaga tatacatgaa aacattgaag atggtatcac
    60481 gttgttaatt gttatggccg tagttcttgt tttactagta atatttggtt tcattagcgc
    60541 agataatatg gctaaacgac aaacaaaaga tatcgaaacg attattcaaa aaatttacta
    60601 tgccaaaaat cgtcatctag gtacatatac acctttaaaa aacaacagtg aactagaaga
    60661 aattaataat tatatttacg atttatttga atcaaatgag caactgatcc attctatcga
    60721 gcatacagaa cgtcgcttgc gtgatattca gttaaaggaa atagaacgcc aattccaacc
    60781 tcatttccta ttcaatacga tgcaaacaat acaatattta attacgttat ctccgaaatt
    60841 agcacaaact gtggtccaac aattatcgca aatgttacgt tactcattac gtacaaactc
    60901 acatacagtg gaattaaacg aagagttgaa ctacattgaa cagtatgttg cgatacaaaa
    60961 catccgtttt gatgacatga ttaagctcca tatcgaaagt tctgaagaag cgcgtcacca
    61021 gacaattggt aaaatgatgc ttcaaccact catagaaaat gctattaaac atggtagaga
    61081 tactgagtca ttggatatta ctatacgcct gacattggca cgtcagaatt tacatgtttt
    61141 agtttgcgac aatggtattg ggatgtcgtc atctcgcctg caatatgtac gccaatctct
    61201 taataatgat gtatttgata ctaagcattt gggattaaat catttacaca ataaagcaat
    61261 gattcaatat ggttcacatg cacgactcca tattttttca aagcgtaatc aaggtacgtt
    61321 aatttgttat aaaataccac tttcaagggg gaatgtagat gtttaaggta gttatttgtg
    61381 atgatgaacg aattattcga gaaggtttaa agcaaatcat tccttgggga gactatcatt
    61441 tcaatacaat atacactgct aaagatggtg ttgaagcatt atcactaatt caacaacatc
    61501 agccagaatt agtaattact gatattcgta tgccacgtaa aaatggcgtc gatttactca
    61561 atgatattgc gcatctagat tgtaatgtca tcatattatc gagttacgat gactttgaat
    61621 atatgaaagc cggtattcaa catcatgttc ttgattattt attaaaacca gtggaccatg
    61681 cacaattaga agttatttta ggaagacttg tccgtacctt attagagcaa caatcacaga
    61741 acggccgtag cttagcatct tgtcatgatg ccttccaacc attattaaaa gtagaatacg
    61801 atgattatta tgtaaatcaa attgttgatc aaattaagca atcttatcaa acgaaagtga
    61861 ctgtttcaga tttaattcaa catatcgatg ttagtgaatc gtatgcgatg cgaacattta
    61921 aagatcatgt tggtattaca attgtcgatt atttaaatcg ctatcgtatt ttacaatctc
    61981 tgcaattgtt agatcgacat tacaaacact atgaaattgc agacaaagta ggtttttcag
    62041 agtataaaat gtttagctat cattttaaaa aatatttaca aatgtcgcca agtgattatt
    62101 gtaagcaagc aaaatagatt agcacataac taatgattat ggcgcctaca ttttgttcta
    62161 tgaaaaagcg gactgataga gaagttttca cttttctttc agtccgcttt ttagagagtt
    62221 taaatataaa acaatactcc ctacaatatt gacaccgcta tatgtcttta ttgtaaggag
    62281 taaattcatt gagtcaccct aagtcgggca aataaggata cttcataaca gggaattatg
    62341 atctgccccg agacatagag atgatatatt tgcaagggta gaagcagtat ctttcatctc
    62401 ttcacgcaca tgccgtcggg gcaggattgc caactatatt gcaatagatt ggcgatactt
    62461 ttaatccgat ttatattaaa tttttaaact tctaattttc ttttcttcat agaaagcaac
    62521 gattcctaat agaatcatgc ctaggaataa tgcaacatag aagacgatga aaacatctgt
    62581 ccatccactt aatgtatatc caaagatgtt taaaccgtta cgtgttggat cagcaatagc
    62641 cgccaaacca actttcgcca ttgagtcacc gaatagatac gcgaatgaac ctgtcattcc
    62701 gtttgctaca ctgatggcat ttttaggaac aaaaccagtc aatgatacac caattaataa
    62761 ttgcggacca aagattaacg cacctaatgc aaacaatgaa atgttaacca tcatgacact
    62821 tgtagcattt gtgtagaata agacaacaaa tgtaatcata aacatacagc caatagctac
    62881 aattgcacga cgacctttta ataagtctga tacgtagccc cataataaac ttgcaactaa
    62941 tgcaccaatt tcaaagtaga atatcgtatt aactgcatcg cctttactaa agtgtaaatg
    63001 ctctgacaca tataacggtg cccagttatc aataccgatt cgtacaatgt atacaaagac
    63061 gtttgaaaca catagaatcc atataacagg atttcccagg atatattttt taaagatctc
    63121 ccatttcgtc ataccttgag aatcaatatt ttctttatcg accggctctt cccaaatttc
    63181 ttcagcacga ttccatccta attcttctgg atcatctttt ccgataaata atgttgcgat
    63241 accaataagt aatgcaatca ccgatgggaa aatgaacatc cctataacat ttccatggaa
    63301 gaatacatta gcaccccaaa gtgcaacacc acctgcaatg gcaccaccga tattatgtga
    63361 tgtattccaa aatcctaagt atcggccacg tttcgttctt ggcgcccatc ttgaaatcgt
    63421 tgaataactt gcaggtccac caactgattg gaacaccccg ttaagtcccc aaagtacaat
    63481 taataatccc attacagaac caaagtaact taaaacaaat cccataatta aaactgtaat
    63541 cgcagataag ataagtaaga acgagataat acgttttgtg ttacgtccat cgacaaaata
    63601 tccaagtaat gttttcccta aaccgtacgt gatactaaat gctaatccga tataaccaag
    63661 ttctaatgta gataatccaa tttcctcttt taaaaacggt tgggccgcct taaagttgtt
    63721 tcgaattaaa tacatagcca tataaacaaa gaacactaca aagaaagctt gcatgaagtt
    63781 tctaagccat aatttacgtt gtaccgataa tggaatgcct ttgttcggaa tcttatggat
    63841 atcaaaaaag ttcatctctg tcacctcaat cattttcgtt aagccaaata tatcacgtcg
    63901 gtgtaagcgc ttaatatcct taaaattatt aaattatcca aaatactgaa cataaacaca
    63961 tacttagaaa catatttatg tactaaaatt aataaatagt taatagtatt tgacaagttt
    64021 tatctataaa atactttatg ttgtcaaaat caacgcttat acatatgaca taatgacgga
    64081 ggacatcatg attctatcta tattacttat ctttttctgt atcagacttg tcagcttaaa
    64141 gatatctatt aatcactcaa aacaattaaa agcagacggt gcagttgaat atggcgttaa
    64201 aaattcgaag tttttagcaa taacacacgt tttaatttat gtattggctg gtgtagaggc
    64261 atttattaat aaagatacat ttagctttgc aaatggtatt ggcttagtta tattaatctt
    64321 tgcttatatc atgttattta tggttattaa aactttaggt ggtatttgga cattaaaatt
    64381 attcatttta cctaaccatc ctattattaa atctggatta tataaaatta caaaacaccc
    64441 aaattacttc ttaaacatca ttcctgaatt aatcggtgta ttattattaa cacacgcaac
    64501 atatacaact attttattag taccgtatgc gtatttctta tatgtacgta ttaaacaaga
    64561 agagaaatta atgaatattt aaaaagatga aagcctgtca tcacattagt cgacatcgac
    64621 gtccatcttt aaagtgcgtt ctgaaatatt atattcagaa gcctttaaag tatgtcgtgt
    64681 ctatgaccat tgtgacaaca ggcttttttt attatcatat acctcactat cgaaaaggtt
    64741 atatactctt ttcaatggtg aggattgtta ctgtgaatgg gtatgaccat cgctaaacac
    64801 ttatataaag tacaccttct ttacaaacaa tgacatgatt taaggtgtga aatttttggc
    64861 aatggcttgg cgacatatgc atttattcaa cactgccacc agtcttactt cagacaaact
    64921 tcagactaac ctcgagttcg tattatgccc accacatgtc tgctggcttg tcgtaaatat
    64981 taacagattt caaagtttga caagcttttt ggaaaccttc ttctactgac ataataggat
    65041 cttcatgttc aatacttaat acataatcat atccattaat aataagttga cttatgatat
    65101 ctgcccatac atatggacta tgtccataac caactgtacg gaatgtccat gctcttgtcg
    65161 caacgttacc atatggttgc atatcagtta gaccatacat atttacattt tcttgattaa
    65221 tatacgtatc tttagcatgg aagtgatgaa ttgcatttgc ttggcctaat atgcgaatcg
    65281 cagcaattgg gtcaatacct tgccaccata gatgactagg atctaagtta gcaccgatat
    65341 attcatttgt agcctcacgt aacttcaaca ttgtatatgg tgtatgcact aaaaatcctg
    65401 catgcaactc tatggcaatt tttacatctt gctcttttgc aaactcagct aaatcttgcc
    65461 aatatggtat caacttttca ttccactgat aatcataaat ttcagagtag gctgttggcc
    65521 aaggtgtaac aggccaatta ggctttttag cggtatcatc tgatcctgca atgccagaaa
    65581 atgtattaac aactggcacg tctaatagat ttgctaaacg gattgtttta cgtaacgttt
    65641 catcggcttc tttcgcttct atcggatctg gagaaattgg attgttatga caactgaaac
    65701 cacttatttg taagcctctg tctgtgattg acttcataaa tgcttggcgc ttgtcttcat
    65761 tttctaacaa ctcatctaac ttacaaaatt tatctcctgg gttaccacct gttccaactt
    65821 caatcatatc caatccagat tctgagacat aatctaacat atcttcaaaa tttttatcgt
    65881 aaaataatac tgaaaataca cctattttca ttgaaaagca ctccttcttt ttatattgag
    65941 aattttgtat catatatgat aatcattaaa gttgtatgct cttgttatca agatcgctac
    66001 gataaatcgc ttctataagg gcatttacat tgcgtgcttc ttccggtttc accacaatct
    66061 cttctatccc taaacacgca ttgacaaagt tacgcgcctg tctctcacca gcaatgtctt
    66121 cgttatgctc aacattagct ttgctttcaa aaatagttcc aaagcggggc tcatatattt
    66181 caaatggaaa taaattgata ccgccatctt ctcctgataa actaacgtga accttatctt
    66241 ctttgatatt tgcagaccac gaacattcaa actgcatgct tgctcgattg gcaaatgtca
    66301 tataactagt aacatgatca tcgacatcaa atttagtatg atcaaatgtt ccccaatcat
    66361 taatttgatt cggttgtttg ctcaattgat tatatgtttt tcctagcact tcatgcggca
    66421 ccatatcttt acctagtagc cacaaagata agtctaacaa gtggcaacca taatcgatta
    66481 aactaccgcc accttgcaac gctttattgg taaaaacacc ccagccaggc actttacgcc
    66541 tacgcatcgc ttgtacacgt gctactaaag gtttaccaac cacacctgat tcaattgctt
    66601 ttttagcagt aattgccaca tctgtgtgac gataatgata tgcgacagtt aataatttgt
    66661 gatttttatt agccgcttca atcatgcgat cacactcttc cgtcgtcatc gccattggct
    66721 tttcacacaa tacatggaca ccatggttca atgcttctat agaaagatca gcatggaatt
    66781 tattaggtgt acaaatgacc accgcatcaa caagtttaaa cagctcgcta ggtgtctcaa
    66841 ctgcatgagg tatattaaag cgcttcgcaa catcaatcat ctgcactgta ttaatatctt
    66901 gtactgcaac taatgagact gtgtctttga gtttcagcaa tgctggaata tgacggtctt
    66961 gtgcaatacc accaacacct atcacaccaa cttttaattt tgtcatgatg tgcctcctta
    67021 ccgtatgatg ttattcaaag taaattgctt tgcctgattt tgcagactga taaatcgctt
    67081 caagaatttt tgtaactacc attgcttgtt ccggtttcac aactggttca gtatcattta
    67141 caactgcatc aatccaagct tttgcttctt cttcagcttc atccacttca ttaccttcat
    67201 aaaagtcgac gcctttgttt tccaattcaa cgtgtttggt ataaagtgta cccatgtctt
    67261 caccatgaat acgtagacca tctttcatat cagcacctgc tttagttcct gataatgaac
    67321 attttgcctc atccacttct aaagaattaa tcgcccaagc ggattctaac atgatcgtcg
    67381 ctccattctt cattttaata aatccaaacg cagaatcttc aactgtaaat tcatctggat
    67441 tccatgaacc ccaagcgttt gccgcatgat gctgtttatt taatttatgg aatgttgaac
    67501 ccatcactga ttctggttca taattatcca tcatccataa cgttaaatct aaagcgtgtg
    67561 taccgatatc gattaatggt cctccacctt gagcttcttc gtctagaaag acaccccatg
    67621 ttggtactgc tcgacgacga atggcatgtg cctttccgaa gtaaatgtct cctaagtcgc
    67681 cacgttgcgc tgcttgatgt aaaaattgac tatctgctcg gaaacgattt tgataaccta
    67741 ttgttaattt tttacctgtt gatttagctg tatctatcat tttttgagct tctgctgtcg
    67801 ttttagccat tggtttttca cacatcacat gtttaccagc atgcaaccct gctacagtaa
    67861 tttcacaatg cgagtcattt ggcgtacaaa catggataac atcaatcgtg tcatctttta
    67921 acaatgcttt gtaatcatca taaacctttg cattgtcagt tccgtatgct tctgccgcac
    67981 tcgctgcttt cgaaatgtct acgtcacaaa atgcgatcat ttcaacattt tcaacttttt
    68041 gtaaacttgg catgtgcttg ccattcgcaa taccaccaca cccaatgatt ccaactttaa
    68101 tcgtcatcat aacgtcctcc ttttaatctt tatcgtcatt agtggttctt tatcaattct
    68161 gccccactcc taatttcaaa ctaacctttt gtcgcacctg ttgttaaacc tgatactaaa
    68221 tagcgttgca agaacaagaa tacgattgct ataggtactg caatcataat tgcccctgct
    68281 gcaaacactg tgaaattatt tgcatactta tcattaataa agttgaacaa tccaactgct
    68341 aatgtgaatt tttcaggact tcttaatagt attttaggta atataaagtc cataaatggc
    68401 cccataaaat tgaacaaagc aacaactgct aaaatcggct tagctaatgg aagcataatt
    68461 tgtaagaaaa tacgcatatg ccctgcacca tcaattttgg cagattcatc aagttctttt
    68521 ggaatcgtat cgaagtaacc tttcactaaa aaggcattca tcggtattga tccaccaata
    68581 tataccagtg ttagtccaaa taaagaatct aataatccaa ttgtatttag caaaatatag
    68641 attgcgacca ttgccattaa tacagggaac atttgtaaaa tcaaaaatgt aatcagcccg
    68701 tatttacgac caacaaagcg atatctagaa aaagcatatg ctgttaacgt gacaaatatc
    68761 acactaaaca gtgcatttgc agatgctacg ataagcgtat ttttatacca agtcaggtat
    68821 tgactactgt catcgaatag taagaatgca taatttttaa atgttgcatt gtctggtatc
    68881 attttggcac catacaagtt cgtacctgga ttaagggaaa tgccaaatgt ccacagtagt
    68941 ggatataaaa tgatgacaaa catcatcgct ataaaactgt aaataccgat tgcttttaat
    69001 atgtttttct tctttgtcat cttataaacc tccctcatct ttaaacgtac ttgtacgtct
    69061 gaattgaata aatgcgacaa tagcaacaat aaatccaata attaatgaca caactgcacc
    69121 catgttgaag ttgttaaact caaatgtcag attatacacc caagatatca agatatctgt
    69181 actaccagca ttctgccctg acactggtgg accgccttta ttaaatagat aaataagatt
    69241 aaaattattg aaattacctg catattgcat aattaacaat ggcgctgtgg cgtaaatgac
    69301 atgcgggaat gtgatgtttc taaacttttg ccaactagac gcaccatcca tatctgctgc
    69361 ttcgtaccaa tctgatgaaa tactttgcag tactccagtg aacagtgcaa agacaaatgg
    69421 gaatccaagc catacttgaa tgccgattaa tgccactttt gcccaaaacg gatcacttaa
    69481 ccatgctggt gctacaccta ataaaggttg caaaatatca ttatttatcg caccaaattc
    69541 atcattaaat aacgctacaa atattaaaat tgtcacaaat gatggtacag cccaaggtag
    69601 gattaacaca gtacggataa atttcttacc tttgacgaca gggtgattta caataattgc
    69661 caaaaacagc cctaatgcaa tttgaagtgt cgttgcaaca agcgtccata ctaatgtcca
    69721 agtaataaca ctgaaaaatg ttttacgcca aacgccaatt gtgaataacg ttttaaagtt
    69781 atctaaacca acccattcta atgtgtgtct cggaggcgcg ttgtataaat tgtaatttgt
    69841 aaatgctact ccaaacataa atattaatgg aaatacaact acaaatatca ataagaatgt
    69901 acctggtgag attagtaagt atgggaacgt cttgtcccat gttgccacca tacgcgcctt
    69961 cggatccttt atttcctcat tgcgattaaa tcgttcagca ttacgatatg catctaaaat
    70021 attaatgata tatagcatga ttgcgaaagc aacgagtaag atagaaataa taccttgtgc
    70081 aagtaagaca cgagaatcgt ctaacttagg tactgtccct aatgtgaaca atccccaaaa
    70141 accaatattt aaaaagctat aaaaaacaga aataaaactg atgaaaaaga taaaaaatat
    70201 cgtcccttta atgggtcttt tattataaaa ctgtcccaaa ccaggtataa cagataataa
    70261 tgccgctaat ttagggttac gtttcgtcat aactaatctc ctttcttatc attttgtgat
    70321 ggatgaagaa tcttaatatt ttgcgttata tcattcgtcg cctcatctaa cgcttgttta
    70381 ggattcttac catttgaaat aaatatgctt gcattgccca tcggttccca aacttgtcgc
    70441 atttcaggaa tattaggcat cggttcagca tgacgtgctt gcttttcaaa cacttttaaa
    70501 tttggattag atgatttcac gtcaacacgt ccagtaattt cgctcatttc atctgtatat
    70561 ttttgtaatg tatctttact agtgatatac agcattaaat ctttagccca atacttatgt
    70621 ttactatatt cagataaata ccaaccacgt acacctagaa atggtttcat aggtttgcca
    70681 ccatctgtag gtaatgttgt tactcctaaa tctttaccaa acgtttcttg atattcatta
    70741 atgttccacg gtccagtgac aaattgtcct acttttcctt ctttaaaaag accaatcatg
    70801 acatcatgtg ttgctgcctt aggaagatac cctttgtcgt accatttttg taatcgttca
    70861 gcattcttga cgacacgttt tgaatttagt cctagctgat gaatatcata ttcactgcca
    70921 tttttcttga aaatataatc atcattgccg aataaaaacg gataattaaa atagaaattt
    70981 ttagcatcaa ataacatacc gtattgtttc tttttactat cagttagttt ggcagcatta
    71041 gcttcaactt cttctaacgt ttgcggtgca tttttcacta attttttatt ataaaaaagt
    71101 gcggttgttt caacgatagc tggcaatgct agttgcttat tgtcataatt catcgcttta
    71161 agtgcttgct tattgaaacc tttcaactca tcttttgata atttgatttc agcagctaag
    71221 ccttgtagat aggcacttcc agtattatca tgtgctaaga aaaagatatc tggacctttt
    71281 cctgcaggag cgtctagcga aatattttct agttgatcat tttgaccaat atttacaagc
    71341 tttactttga tgccagtttt tttagtatat tgatccgtaa tttttttata aaacgccatt
    71401 tgcttgtcgc catccaccca catcgtaagt tggttaggct tgtctttaga attatcttta
    71461 ttcaatgctt tatcaatatc ttctttcgaa cgattagggc cacatgcagt tacgattaat
    71521 aacattacca cggctaacgt gatacatttt aaaattttag acatgaatac tcccccttag
    71581 acgatacgat ttcctgtttt ttcatcaaaa aagtgacact tattcatatc aaatgctagt
    71641 gtaatcttgt cgttcgccat cacttgagtt cttgaatcta atttagaaat caattccatt
    71701 ccttggaatg tgctatgtac cataatttct gaacctaaca gttcggatac aactacttca
    71761 gattcaaatt gtgtctctgg agaagtttga ataaatattg gttcttcatg aatgtcttca
    71821 gctcgaatac ctaaaataat ttccttgtct aagtagccag cagcttttaa cttttcaaat
    71881 tttttattat gtaatttaaa atggtgtgtt cctaccttca atccgtccat ttcaactgta
    71941 gcattcaaca tattcattgc tggtgagccg ataaattgcg ccacaaatat gcaattaggg
    72001 gcatcatata tttctctagg tgtgccgact tgcataatgt cgccatcttt caaaacaaca
    72061 attcgactag ccatcgtcaa tgcttcagtt tgatcatgtg taacataaat tgtcgtagta
    72121 ttaagtcgct tatgtaattt caatatttct gtgcgcattt gtactcgaag cttcgcatca
    72181 agattcgata atggttcatc cattaaaaag actttcgcat ccctaacaat agctctgccc
    72241 aaagcaacac gttgacgctg tccgccagat aacgcttttg gtttacgacc aagatactca
    72301 gttaatccta atatttcagc tgcttcatta actttttgtt caatctcttt tttatttact
    72361 ttacgtagct ttagcccaaa tgccatattt tcaaaaacag tcatatgtgg atataatgca
    72421 tagttttgaa ataccatcgc aatatctcta ttctttggtt caacatcgtt catgcgttcc
    72481 ccatcaatat aaaaatctcc agatgtgata gactctagtc cagcaaccat tcgtaatgtt
    72541 gttgatttac cacatcccga tggtccaaca aatacaatga attctttgtc agtaatatgt
    72601 agattaaaat ctttcactac agtattgttg ttatcatacg tctttttaat atgctctaac
    72661 tttagttctg ccatgttatg cctcccatac tttgtttaca gtttgattgt gcaatacact
    72721 ttgtgaaaac gtttgcacaa atacttacat aaatcatata ttcagtcatt attaatgtca
    72781 atcataaatt gtaaacgttt acattactgt cagaattaaa cttttataca gaaaattgat
    72841 atagtgcaca actattttct agtgtgacaa taatgttttt tatgtgcgat atttcactgc
    72901 caatgctaac aaccttgtct tacattttaa gtaatatcta aatcaaacaa gtatgactta
    72961 gcattacgat tttcattgaa aaaagcaacc attatgtaag tatcatactc acacaatgat
    73021 tgcttaattt tttagactgc gggcaccctt ctataactac aaatcatata ggctacgttc
    73081 cccatcaggt aactgatcga taaacggttt tggatcttct gcatatgcat tacctacgcc
    73141 acccttcatt ctttgaaaat gtaaatgtgc gcctgtcgtt tgtttacctg tattgcctga
    73201 atatgcaata atatcacctg ctttgactcg atcacctact ttgacattat atttgtctaa
    73261 gtgtagatac cactggtgat attctccatt gtcttcggca atctgtaata ccttgccgcc
    73321 caattcatta ttaaagatac gtgttacttt accattcgtc ggcgctttaa ttggtgtacc
    73381 ttttggcaat gcaaaatcaa tgccataatg cttaccatca aatggtgaat gttgatattt
    73441 accaaaagtc tcagtaatcc gactccctct aaaaaagtct tcccattttg agtgtccttg
    73501 tcctttatga ttttgtgtca atttatcggt atattggtta aaccattttt gaatgtcatc
    73561 tatataaata atcgtaaata atagtgaaag aaaaatgata attgttgcca aaatacgttt
    73621 tggtcgcttt gtcatagtgg catccatcct tcatatattt tgggtattaa gtatatattc
    73681 taaatcaact atttcataat taaatgcact
//
LOCUS       DAMZLE010000003        61823 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMZLE010000003 DAMZLE010000000
VERSION     DAMZLE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 61823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.003"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     misc_binding    165..534
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            600..1703
                     /locus_tag="POB92_000157"
     CDS             600..1703
                     /locus_tag="POB92_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6577683.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETFFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYNIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            1700..2860
                     /locus_tag="POB92_000158"
     CDS             1700..2860
                     /locus_tag="POB92_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDI6577684.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            2829..4670
                     /locus_tag="POB92_000159"
     CDS             2829..4670
                     /locus_tag="POB92_000159"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDI6577685.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIHEGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDNAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            4667..6895
                     /gene="metE"
                     /locus_tag="POB92_000160"
     CDS             4667..6895
                     /gene="metE"
                     /locus_tag="POB92_000160"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI6577686.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTINDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            6938..7690
                     /locus_tag="POB92_000161"
     CDS             6938..7690
                     /locus_tag="POB92_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDI6577687.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(7773..8954)
                     /locus_tag="POB92_000162"
     CDS             complement(7773..8954)
                     /locus_tag="POB92_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDI6577688.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            9493..10626
                     /locus_tag="POB92_000163"
     CDS             9493..10626
                     /locus_tag="POB92_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDI6577689.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(11016..11645)
                     /locus_tag="POB92_000164"
     CDS             complement(11016..11645)
                     /locus_tag="POB92_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDI6577690.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIVASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(11645..12487)
                     /locus_tag="POB92_000165"
     CDS             complement(11645..12487)
                     /locus_tag="POB92_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577691.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPRQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEITKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(12512..13225)
                     /locus_tag="POB92_000166"
     CDS             complement(12512..13225)
                     /locus_tag="POB92_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDI6577692.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYTNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     VIQTIIAFVVLLIFVVGQAEANAMLFFLIPFFASAIFNTQFTLFNRKFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(13222..13425)
                     /locus_tag="POB92_000167"
     CDS             complement(13222..13425)
                     /locus_tag="POB92_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6577693.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEV"
     gene            13665..14054
                     /locus_tag="POB92_000168"
     CDS             13665..14054
                     /locus_tag="POB92_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDI6577694.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSTIPVAQKSNQSIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            14153..14377
                     /locus_tag="POB92_000169"
     CDS             14153..14377
                     /locus_tag="POB92_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDI6577695.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            14394..15095
                     /gene="tatC"
                     /locus_tag="POB92_000170"
     CDS             14394..15095
                     /gene="tatC"
                     /locus_tag="POB92_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDI6577696.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCLISPVMFYQLWAFVAPGLHNNERQFIYKYSFFS
                     VLLFIFGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGILFQL
                     PILFIGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     TFIVKFTYRGKPPTH"
     gene            complement(15187..16899)
                     /locus_tag="POB92_000171"
     CDS             complement(15187..16899)
                     /locus_tag="POB92_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDI6577697.1"
                     /translation="MKHYLIKFVAMLITAAMVCSFGLLKSQAAKQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAVMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSDTSAKDQYHVSQLNDELEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPTYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(16880..18109)
                     /gene="efeB"
                     /locus_tag="POB92_000172"
     CDS             complement(16880..18109)
                     /gene="efeB"
                     /locus_tag="POB92_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDI6577698.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDKVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETSRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(18106..18960)
                     /locus_tag="POB92_000173"
     CDS             complement(18106..18960)
                     /locus_tag="POB92_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577699.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANIEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(19227..19772)
                     /locus_tag="POB92_000174"
     CDS             complement(19227..19772)
                     /locus_tag="POB92_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6577700.1"
                     /translation="MFGMKVNEQIILKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFSDGNGFQCGIWYEGTLVGVIGLHEINRMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            20077..21072
                     /locus_tag="POB92_000175"
     CDS             20077..21072
                     /locus_tag="POB92_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDI6577701.1"
                     /translation="MASLKNKHFMIGLTLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSNGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(21132..21698)
                     /locus_tag="POB92_000176"
     CDS             complement(21132..21698)
                     /locus_tag="POB92_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6577702.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21712..22773)
                     /locus_tag="POB92_000177"
     CDS             complement(21712..22773)
                     /locus_tag="POB92_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6577703.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAPTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSIASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSPDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(22787..23713)
                     /locus_tag="POB92_000178"
     CDS             complement(22787..23713)
                     /locus_tag="POB92_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6577704.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNVYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAINTPQMSDLDAIYKKLQQQTQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            24025..25383
                     /gene="glpT"
                     /locus_tag="POB92_000179"
     CDS             24025..25383
                     /gene="glpT"
                     /locus_tag="POB92_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDI6577705.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(25466..25906)
                     /locus_tag="POB92_000180"
     CDS             complement(25466..25906)
                     /locus_tag="POB92_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDI6577706.1"
                     /translation="MYKSKILLKYIFSEEPEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(26013..27368)
                     /gene="mepA"
                     /locus_tag="POB92_000181"
     CDS             complement(26013..27368)
                     /gene="mepA"
                     /locus_tag="POB92_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDI6577707.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQDIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(27475..27894)
                     /gene="mepR"
                     /locus_tag="POB92_000182"
     CDS             complement(27475..27894)
                     /gene="mepR"
                     /locus_tag="POB92_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDI6577708.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            28106..30061
                     /locus_tag="POB92_000183"
     CDS             28106..30061
                     /locus_tag="POB92_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6577709.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            30066..30509
                     /locus_tag="POB92_000184"
     CDS             30066..30509
                     /locus_tag="POB92_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6577710.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            30511..30795
                     /locus_tag="POB92_000185"
     CDS             30511..30795
                     /locus_tag="POB92_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6577711.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            30810..32156
                     /locus_tag="POB92_000186"
     CDS             30810..32156
                     /locus_tag="POB92_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI6577712.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISNGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(32249..33274)
                     /locus_tag="POB92_000187"
     CDS             complement(32249..33274)
                     /locus_tag="POB92_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDI6577713.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFVLSLGAKFNHQAKEGKLLLDMNRNVSNERAKILLGWEPIATQKEAILAAVDS
                     MAKYHLI"
     gene            complement(33590..34612)
                     /locus_tag="POB92_000188"
     CDS             complement(33590..34612)
                     /locus_tag="POB92_000188"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI6577714.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRGITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELVDKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFGNVTVGSL
                     VEMILS"
     gene            complement(34590..35534)
                     /locus_tag="POB92_000189"
     CDS             complement(34590..35534)
                     /locus_tag="POB92_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDI6577715.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETKDVLNRTD"
     gene            complement(35524..36324)
                     /locus_tag="POB92_000190"
     CDS             complement(35524..36324)
                     /locus_tag="POB92_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDI6577716.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(36325..36657)
                     /locus_tag="POB92_000191"
     CDS             complement(36325..36657)
                     /locus_tag="POB92_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDI6577717.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(36691..37692)
                     /locus_tag="POB92_000192"
     CDS             complement(36691..37692)
                     /locus_tag="POB92_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6577718.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(37806..37898)
                     /locus_tag="POB92_000193"
     CDS             complement(37806..37898)
                     /locus_tag="POB92_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577719.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            37994..39193
                     /locus_tag="POB92_000194"
     CDS             37994..39193
                     /locus_tag="POB92_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6577720.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            39266..40093
                     /locus_tag="POB92_000195"
     CDS             39266..40093
                     /locus_tag="POB92_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6577721.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(40335..42407)
                     /gene="lip2"
                     /locus_tag="POB92_000196"
     CDS             complement(40335..42407)
                     /gene="lip2"
                     /locus_tag="POB92_000196"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDI6577722.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTN
                     AAVQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQLSKYQSTQ
                     NSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            42825..44138
                     /locus_tag="POB92_000197"
     CDS             42825..44138
                     /locus_tag="POB92_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDI6577723.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFPTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            complement(44269..44937)
                     /locus_tag="POB92_000198"
     CDS             complement(44269..44937)
                     /locus_tag="POB92_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI6577724.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYNHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            45078..45878
                     /locus_tag="POB92_000199"
     CDS             45078..45878
                     /locus_tag="POB92_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6577725.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLTHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            complement(46155..47015)
                     /locus_tag="POB92_000200"
     CDS             complement(46155..47015)
                     /locus_tag="POB92_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI6577726.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            47173..48054
                     /locus_tag="POB92_000201"
     CDS             47173..48054
                     /locus_tag="POB92_000201"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDI6577727.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            48094..49626
                     /locus_tag="POB92_000202"
     CDS             48094..49626
                     /locus_tag="POB92_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDI6577728.1"
                     /translation="MKEIGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKMAT
                     KK"
     gene            complement(49732..50952)
                     /locus_tag="POB92_000203"
     CDS             complement(49732..50952)
                     /locus_tag="POB92_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6577729.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(50963..51886)
                     /locus_tag="POB92_000204"
     CDS             complement(50963..51886)
                     /locus_tag="POB92_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDI6577730.1"
                     /translation="MANLQKYIEYSREVQQALENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            complement(51861..52979)
                     /locus_tag="POB92_000205"
     CDS             complement(51861..52979)
                     /locus_tag="POB92_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6577731.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            complement(53322..54353)
                     /locus_tag="POB92_000206"
     CDS             complement(53322..54353)
                     /locus_tag="POB92_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6577732.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            complement(54561..55238)
                     /locus_tag="POB92_000207"
     CDS             complement(54561..55238)
                     /locus_tag="POB92_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577733.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            complement(55251..56300)
                     /locus_tag="POB92_000208"
     CDS             complement(55251..56300)
                     /locus_tag="POB92_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDI6577734.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQAEAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            complement(56550..57440)
                     /locus_tag="POB92_000209"
     CDS             complement(56550..57440)
                     /locus_tag="POB92_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDI6577735.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKVNLGNQNIMAVAWYQNSAEAKALYLQGYNSAKVQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKDVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            58024..59331
                     /gene="brnQ"
                     /locus_tag="POB92_000210"
     CDS             58024..59331
                     /gene="brnQ"
                     /locus_tag="POB92_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6577736.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVSLLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            59570..60394
                     /locus_tag="POB92_000211"
     CDS             59570..60394
                     /locus_tag="POB92_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6577737.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(60644..61042)
                     /locus_tag="POB92_000212"
     CDS             complement(60644..61042)
                     /locus_tag="POB92_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6577738.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(61192..61566)
                     /locus_tag="POB92_000213"
     CDS             complement(61192..61566)
                     /locus_tag="POB92_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDI6577739.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
BASE COUNT        19448 a        11780 c         9460 g        21135 t
ORIGIN      
        1 attaaccaca ctttatttaa aaaataattc atttttcaac ttcaagatac caattacaat
       61 ttcacaaaca aattaacttt taaatcaaaa ttttctgaat aattgttgac aaatcttttt
      121 ttactctgta agctaattaa tatcttataa cagtttaatg aaacgtaaac acaataaaga
      181 ggaaagtaaa acacaccctg cttatacaga gagtctttag tagctgagag aagattttga
      241 aagcgtgttt gaaaatggcc ttggagtgtt gatgccaata tgaggtgtct acgggttcgc
      301 ccgttatagc gatacagtat taacattgat gttaaatggc gtactggatt ctttacgcac
      361 gattttttgt taataagtat gggatagcac attactatat ccttacttac tgactttaat
      421 tgtgataatt gttcagtaag catatttact tttaatgcgt actgaataag gttatttcag
      481 cgatggaata acaaataaag gtggtaccgc gaaacataag ctttcgtcct ttttatccga
      541 ttcattcggg tacgaaggac ggaagctttt tttatttttt cttataggag ggtcttaata
      601 tgaaggatac acagttagcc caaatcacat taaccgatga ttcaaccggt gctatagcga
      661 atccaatcca tttatctact gcctacaagc atccaaaact aggacaatcg acaggttttg
      721 attatacacg tactaaaaat cctacacgct caacatttga aacctttttt gccaaactcg
      781 aacatggtat tgcatcattc gctacatcaa gtggaatgtc agccattcaa ttaatatgta
      841 atttatttaa accacacgat gaaattttag tttcattcga tttatacggc ggcacattta
      901 gattatttga attttatgag caacaataca atatcaaatt taagtacgtt gattttacag
      961 attatgaaca agttgaaaaa gaaatcactg ataaaacagt tgcattattc attgaaccaa
     1021 tatctaaccc acaaatgatt gctattgatg taaagccata ctatcaactt tgtaaagcta
     1081 aaggcttatt gtcaattatc gacaatactt ttttaacacc ttatctttca acaccactag
     1141 cagaaggtgc tgatatagtc ttacattcag ccacgaaata tattggcgga cataacgatg
     1201 tactagcagg tgtcgtaacc gtcaaagatg aatcactcgc gcaacagttg tttgattttc
     1261 acaacatgac tggcgcaaca ctttcaccaa tagatagtta tttgttgtta cgtggactta
     1321 aaactttgca tttacgcatt gagcgtgcgc aatcaaacgc tagaaaactt gctaaaaaat
     1381 gtcagtcact tcaagcaatt gacgaagtac tatatagcgg gcaaactggc atgcttagtt
     1441 taagacttaa caaggcctat agcgtcgcta aattattaga aaatttagac atttgcattt
     1501 ttgcagaaag tttaggaggt actgaaacat tagtgacctt cccttacacc caaacacatg
     1561 ttgatatgcc agatgctgaa aaagataaac gtggcattga tgagtattta atccgcttat
     1621 cactaggtgt tgaaaattat gaagacatcg aacgcgatat catccaagca ttagataaag
     1681 ctcagattgg agagattgta tgacactttc aaaagagaca gaagtgatat tcgattggcg
     1741 tagaggtgtg gaatatcatt cagctaaccc accactctat gattcttcaa cattccatca
     1801 aacaagtctt ggtggcgatg ttaaatatga ttatgcacga agtggcaacc ctaaccgtga
     1861 acttttagaa gagaagttag cacgattaga acagggaaaa ttcgcttttg cttttgcatc
     1921 aggtattgct gctatttcag cagtactttt gactttcaaa tctggtgatc atgtcatctt
     1981 acccgatgat gtatatggcg gtacttttcg cctcactgag caaattttga atcgatttaa
     2041 tattgaattt acaaccgtag atactaccaa gctcgaacaa atcgagggtg ccattcaatc
     2101 aaacacaaaa ttaatttata tcgaaacacc atcgaatccc tgttttaaaa ttactgatat
     2161 caaagctgtt tctaaaatag ccgaaaagca tgaactactg gtagctgtgg acaatacatt
     2221 tatgacaccg ttaggccaat cacctttatt acttggcgct gatattgtca ttcatagtgc
     2281 tacaaaattt ttaagtggac atagcgattt aattgctggt gctgtcatta ctaataatga
     2341 ggcaattagt gaagctcttt atttaataca aaatggtaca ggcaatatgt tatctgctca
     2401 agatagctgg acacttgcta aacatttaaa aacatttcca atcagattta aacaatctgt
     2461 cgaaaacgcg caaaaaatcg tgtcattttt aataaagcaa gatgaaattt cagaagttta
     2521 ttatccggga ctcactactg ctcatttaga acaagctaaa aatggcggtg ccgttattgg
     2581 ctttcgttta gctgatgagt ctaaagcaca acaatttgtc gatgcactga cattaccact
     2641 cgtttcagtg agtctgggcg gtgttgaaac gatcctttca catccagcaa caatgtctca
     2701 cgctgcacta cctgaagaag tgagacaaga acgtggtatc actttcggtc tattccgatt
     2761 aagtgttggt ctcgaagatc ctgatgaact cattgcagac atcaaatacg cattaaagga
     2821 ggcattcaat gagtcaattc ctcacacaat tgaaagataa tgttttagta gctgatggcg
     2881 ctattggaac cattttatac tctgaaggat tagacacctg tccagaagca tataatctta
     2941 gccatccaga taaagttgaa cgtatccatc gttcatatat cgaagctggt gctgatgtca
     3001 ttcaaaccaa tacttatggt gcaaattttg aaaagttaaa acgattcggt cttgaagata
     3061 aagttaaagc tatacatcaa gccgccgtac gcatcgcaaa aaaagcagca aataaagata
     3121 cgtatatatt aggcacagtt ggtgggttta gaggtatcaa acaagaggat atcagcttac
     3181 aaactattct ttatcatact gaaattcaaa tagacacctt aattgaagaa ggcgttgacg
     3241 cgctactttt cgaaacgtat tacgacctag aagagttaac aaatgtcatt tcacgaacga
     3301 gaaagaaata cgacattcca atcattgctc aattaaccgc ttcaaacaca aattacttag
     3361 ttaatggtca ggcaatcaat gaaggattaa aacaactcgt tcaatgtggt gccaacatcg
     3421 tgggactcaa ttgtcatcat ggcccgcacc atatgcaaga gtctttcaca catattgaat
     3481 taccagagca cgcatactta tcttgctatc caaatgccag cttattagat attgaaaata
     3541 gtgaatttaa gtatagtgac aatgcacaat atttcggcca agttgctcaa aatttaattc
     3601 acgaaggtgt tcgtttaatt ggtggttgct gtggtacaac gccagagcac atcaaattta
     3661 ttaaagaatc tattcagaca cttaaacctg ttaatgacaa aaaagtgatt ccgataccaa
     3721 cgaaagcact tttcaatcca tctcaaaata aagttagaca atcattaaca tctaaggttc
     3781 aagaacgtcc aaccgttatt atcgaattgg atacaccgaa acatttagac acggatagat
     3841 tttttgaaaa tatcgctaaa cttgacaaag ctaatgtaga tgcggtaaca ctcgcagata
     3901 attcattggc aactgtcaga attagcaaca ttgctgctgc aagcttaatt aaacaatatt
     3961 acaatattga accactcgta catattacat gtcgagaccg aaacttaatc ggcttgcagt
     4021 cccatttact tggattatcg ctcattggcg ttaacgaaat attagccata actggtgatc
     4081 cttcaaaagt tggtcactta ccaggtgcaa ccaatgtcta tgatgttaat tctaaaggat
     4141 taactgaact cgctctaaga tttaatcaag gtattaacac tgacggtgat gcgctgaaga
     4201 aacgtacaca cttcaacatc gctggcgcct ttaatcctaa tgttcgaaaa ttagatggtg
     4261 ccgtcaaaag attagagaaa aagatagaaa gcggaatgtc ttattttata acacaacccg
     4321 tgtacagcaa agagaaaatc attgaaattt accatgccac taagcacttg aacaaaccat
     4381 ttttcatagg cattatgcct atcgcaagtt acaaaaacgc actctttttg cataatgaag
     4441 tgccaggaat caagatgtca gatgaaattt tacaacaatt tgaagcagtt aaagatgata
     4501 acgccaaaac acgagagcta agtcttaagc tttcaaagga tttaatcgat actgttcatg
     4561 aatattttaa tggtttatac attatcacac cgtttcaaaa tgtcgaagat tcattagaac
     4621 ttgcagcata ctcaaaatct attactgctc acaaggaggc aatattatga caacaattaa
     4681 aacatcaaac ttaggattcc caagattagg tagaaaaaga gaatggaaaa aagccatcga
     4741 aagttattgg gcaaaaaaga tttctaaaga agaattagat caaacattaa ctgatctaca
     4801 taaagaaaat ttattattac aaaagtacta tcacttagat agcatcccag ttggtgattt
     4861 ctctttatat gaccatattt tagatacgtc attattattc aacatcatcc cagaacgttt
     4921 ccaaggtaga actatcaatg atgacttatt atttgatatt gctcgcggta ataaagacca
     4981 tgttgcaagt gcacttataa aatggtttaa cactaactat cattacatcg tacctgaatg
     5041 ggacaatgtt gagcctaaag tgagtcgcaa tgtattatta gatcgcttta aatacgccca
     5101 atctttaaac gttaacgctc accctgtcat tgttggtccg attacttttg ttaaattatc
     5161 aaaaggcggt caccaaactt ttgaagaaaa agttaaaacg ttattaccac tttataaaga
     5221 agtgtttgaa tcattaattg atgcaggtgc tgagtacatt caagttgatg agccaatctt
     5281 agttacagac gacagcgaaa gctatgaaaa tattacacgt gaagcttatg actatttcga
     5341 aaaagctggt gttgctaaaa aattagtcat tcaaacatac tttgaacgtg cacatcttaa
     5401 attcttaagt tctttacctg ttggtggttt aggtttagac tttgtccatg ataatggcta
     5461 taaccttaaa caaattgaag ctggagattt tgataaatca aaaacattat acgctggaat
     5521 tattgatggt cgtaatgtgt gggcaagtga cattgaagct aaaaaagtct taatcgataa
     5581 attgttagca cacactaatg aacttgtcat tcaaccatca tcttcattat tacatgttcc
     5641 agtatcttta gatgatgaaa cattagatac aagtgttggc gaaggcttaa gctttgcaac
     5701 tgaaaaatta gacgaattag atgcattgcg tcgcctattt aatcaaaacg atagtgttaa
     5761 atatgataaa ttaaaagcac gttatgagcg tttccaaaat caatcattca aaaacttaga
     5821 ttatgatttc gaaagcgtgc gtacttcaag acaatcacca ttcgcgcaac gtattgaaca
     5881 acaacaaaaa cgtttgaact taccggattt accaacaaca actattggat cattcccaca
     5941 aagccgagaa gttcgaaaat accgtgcaga ttggaagaac aaacgcatta cagacgaagc
     6001 atatgaaaca ttcttaaaaa atgaaattgc tcgatggatt aaaattcaag aagacattgg
     6061 cttagatgta ttagttcacg gtgaatttga acgtaatgac atggttgaat tcttcggaga
     6121 aaaattacaa ggtttcttag taactaaatt cggttgggtg caatcatatg gttcacgtgc
     6181 cgtaaaacca ccaatcattt atggtgatgt aaaatggaca gcgcctttaa ctgttgatga
     6241 aacagtttat gcacaaagct taacagataa accagttaaa ggtatgttaa ctggacctgt
     6301 aacaattcta aactggtcat ttgaacgtgt tgatttacca cgtaaagtcg ttcaagatca
     6361 aattgcttta gcaatcaacg aagaagtatt agcacttgaa gctgcaggaa tcaaagttat
     6421 ccaagttgac gaacctgcat tacgtgaagg cttaccatta cgctctgaat atcacgaaca
     6481 atatcttaaa gatgctgttt tatcatttaa acttgcaacg tcttcagttc gtgatgaaac
     6541 tcaaatccat acacatatgt gttattctca attcggtcaa atcattcatg ctattcatga
     6601 cttagatgct gatgttattt caattgaaac atctcgtagc catggtgatt taattaaaga
     6661 ctttgaagat attaattatg atttaggtat tggtctaggt gtgtatgata ttcatagtcc
     6721 acgtattcca acaaaagaag aaattactac agcaatcaat cgttcactac aacaaatcga
     6781 ccgctcatta ttctgggtaa accctgactg tggtttgaaa acgcgaaaag aagaagaagt
     6841 taaagatgca ttgactgtgc ttgtgaatgc tgttaaagct aaacgccaag aataatcgca
     6901 gcatttatca tataataaac taaaaacggg tgattttatg acacaatatc ctttatggca
     6961 acaattaaat actttaaaac aagcaacttg ggttgattta acgcatacat ttgatccgga
     7021 aataccacgt tttagtgagt ttgaaaaagg cgaagtttca actttattca cggttaaaga
     7081 tcatggtttc tatgtgcaac gttggaatat tgtaacgcaa tatggtactc acattgatgc
     7141 accaattcac tttgtcgaga ataaacgtta tttagaagac attgatttaa aagaattggt
     7201 attgccgtta attgtattag acttttcaac agaagtagcg aacaacaatg atttcattgt
     7261 aacacgtgcg catatagaag catgggaaaa agagcatggt actattgaac ctggcacttt
     7321 tgttgcactt cgtaccgatt ggtcaaaacg ttggcctaat attgaaaagt ttgaaaacaa
     7381 agatgcgaac ggacaacaac atgcaccggg ttggggatta gatgctttga agtacttaat
     7441 tgaagaacgt cgtgttgaag ctgttggtca tgaaacattc gataccgatg catctgtgga
     7501 cgttgtaaaa aatggtgatt tagttggcga acgttatatt cttggtcagg ataaatacca
     7561 agtcgagtta ttaactaatt tagatcaatt accaactcgt ggcgccatca tttacgctat
     7621 cagccctaaa ccaaaagatg cacctgggtt ccctgttaga gcatttgcaa ttaaaccatc
     7681 aaatgactaa tattacccaa taaatccata aacaaataaa aacaggttat gccaacatgt
     7741 aattaatact gttgtgataa cctgttttct tattatttat actttgatac aactgcagcg
     7801 atagcttgac cgccaccaat acacaatgat gttaaaccag tttcaacttc atcattcagt
     7861 tgatgcaata atgtcactaa aactctagca ccagatgcac caatagggtg tcctaatgca
     7921 atagcgccac ctttaacatt caccttttca ggaggtaatt ttaattcacg atcaacagct
     7981 aatgattgtg ctgcaaatgc ttcatttaat tcaaatacat caatatcgct taattctttt
     8041 ttactacgtt tcaaagcctt ttctacagcg ccaactggtg caatacccat aatagaagga
     8101 tctacaccat gacttccaaa gccatcaagc actgccaatg gttcgatatt taattcttta
     8161 gctttgtctt ctgacatgac taacatcatc gcagcaccat cattgattcc tgatgcatta
     8221 cctgctgtaa ctgtcccgtc ttttttgaaa gctggtctta agcgacttaa tttttcgact
     8281 gatacatttt cacgtacacc ttcatcctta gtgactaaga ttggttcacc tttacgttga
     8341 ggaatcgata ctggaactat ttcactatca aattcaccat tttgctgtgc acgtactgct
     8401 ttttgttgtg agtttacagc aaatgtatct tgttcttctc ttgaaatacc atattgctcc
     8461 actaaatttt cagcagtaat acccatatga tattgattaa atacatctgt taaaccatca
     8521 tataccatgc tatcaaccat tgattgatgt cccattttaa aaccgaagcg actgttgttg
     8581 acaagcattg gtgactgaga catattctcc ataccgccag ctagcacgat gtcattttca
     8641 ccagtcacaa tagattgata tgctaattga atcgacttta acccagaacc acatacttta
     8701 ttcactgtaa atgcaggtac tgtttctggc aagccacctt tcatagcagc aattcgtgct
     8761 ggattttgtc cttgtcctgc ttgtagtacg ttaccgatga taacttcatc aatctcactt
     8821 ggattcaaac ccgtctcttt aataatatgt tctattaaag tcgcacctaa atcataggct
     8881 ggcacgtctt taaacgcacc tccaaaaacg ccaataggtg tcctgtatgc tgctgctaat
     8941 acgactctcg tcatgtcata ttcatctcct tatgatatat acaattttat tactaatacg
     9001 tctaatgaac caatacgcca ctaacatcca atttatgaaa ccaattgcta cgtatgttac
     9061 attttcattc ttgttatgca tttaacaaat catattatac aatgatgtaa tttgtttggg
     9121 caatcggaaa cgtaaacggt ttcactttaa catttcacac tcttaaatat gctctatcat
     9181 cctcatctat agtacaaaat tatgctttga tttaccacaa attatcatta atcatttcgc
     9241 gtaatgactc taattaaatt ctatctatca ttgcatcgct attcacagct ggtttcacca
     9301 tcaatttctc atcaattttt tattaccatt acgtctctag tcaaatatta aatcgctgat
     9361 gattgttaat tccgcaatta ccatgcagtc acctctcctt ccacttcatc gccaaatctg
     9421 cccctattca ctagaaaaaa taaatagtat acttagtaaa ttaattacac atcttaaagg
     9481 agttaaaatt ttatggttaa tgcattaaca ccaaaacatt tagctgaaaa gcgtgcagga
     9541 ttccatgagt tgttctttga tttaattttc gtctatgcca ttcaaaaaat tgcacatgtg
     9601 attttaacaa cacaaaacgg ttccatttca gctgatttat ttttcaaata catcgtcatg
     9661 agtttgttct tatggttaat gtggtctcat caaacatttt ttacaaatcg atttggtcaa
     9721 gttacattca aagacgtctc gtttatgatg tttaatatgt tcatcatggt cttcctatct
     9781 aacagcctat atccggactt tgaaaagaca ttcttcccat tcttcttatg cgtggcaatt
     9841 atgtatttaa gtatcggatt acagtattta ttacatatac gtacagggtt agattatggc
     9901 gataagcgta catgtcaagc ttttgcgagc gtagcctttg tgatttcatt tttatcattt
     9961 ctatcattag tactaccaca aagtatccat tacatccctg gatttttagg cgtattcatt
    10021 gcagcgacag gacttattcc atttcagaaa tatcttgtat tatcacctgt taatatgatg
    10081 catttagtag aacgtttttc gttactaaca attattatct ttggcgaggt actggttggt
    10141 ttagcttcgt catcatttag cattgatcat tttagttaca tttatatatt ccaatttatg
    10201 attttaatta gtcttttcgg tgtatattgg atcattactg aaaattacat taatcataaa
    10261 ttgtcatcta tcggtttccg tttatcgtat acgcatctat taatcaatat cgctttaggt
    10321 gtgattaatg ctgcaattgt ttttagtaat aacaataaac taaatgactt gtttgaaatt
    10381 aatatgatgt atatcagtgt tttaatattc tatattggat tatggttaat cacgccttat
    10441 ttccataatg aactgacaaa cgcaaaatat atttcttctt cactaggcat acttgtagta
    10501 tcatatatca tcagccttat ctttaagggg catgaccaag taatgattat ctcggtatcc
    10561 gttgcgactt tctgcatcat gctgatttat tttaaaaatc aacgtctacg taataaacat
    10621 gaataaatag tattcacctc ggtcaaagta tttgaactga atccaaaagt gtcgaacttc
    10681 acattatact tttgtgacaa tcatcagtta tgatacatga tcgagttttt atatttccga
    10741 ggaaatcatt ggcatatacc atcatcaatt gatacacgca aataaatttc agcaacatac
    10801 tcaatgatat atacacgtta atcctcacta taaaaggatg caattcccac acaacctaat
    10861 cttctaaaaa caaatgatat gatatagaga tgtcacctca acctatcatc ccgcatttca
    10921 ttccacaaca ccaaaaagag cagatgataa tcgtattgat ttatcacctg ctctaaatat
    10981 tatttcaatg actagcttca caccaagaat attgattata gttcttgttt gttaaaaatt
    11041 ttaatagaca atatataaga aacacttgtt ataacgatac caatgaccgc atatactatg
    11101 acaactaacc atccttcagt gctaattttt tcaaattcta atgccgatgc accgctaacc
    11161 ataccatata taaagaaaaa gaccacgaaa gaaaaaagta gtaggataga tgcaactatc
    11221 ataatgacat tagagttttc agcgccaaat ttaaatgtca atgggaaaat gatagagtat
    11281 gcccctatga taccaaaact cattaaacct gacataatac caatcatcac actttgggtc
    11341 acgatcgtag tcacaactaa tccaatcatt aaacttgcac cgaataagat taaataaaag
    11401 gcaaagtatg acttaatata atcactacgt ttaaccggta aagtagatac ataatacatc
    11461 catcttgagt ctttttcatg tttaatatta tcagtaatgg gtgtgattaa cataacccca
    11521 gccattgccg aactcatcaa cggattaaat actgcaaagt atcccgcagc tatgatagcg
    11581 actataaaat aaatatatgt ttgctttctc gttgcataaa aactacttag gaacatacct
    11641 ttcattatac ttcacctcgc attatgattt tcgtagcgtc atcaatgtgt tttaaaggct
    11701 ctgcattttc aatttctgca taatctttaa ctaaaatttg atatttcccc tttgacaatc
    11761 tagaagcaat gattaaatgc ttagtaattt caacatcttt atctcctgtc gtaacaattc
    11821 catattgtgc taaaagtata tctttctgtt cagttaaaat cattcgtcca tctttcataa
    11881 acactaattt atccgctaat tgttctatat cttcagaaat atgcgatgat attaagatgc
    11941 ctccaccttg agcgacaaaa tcttctaata gttccattac ttcttctcgt ccagaaacat
    12001 ccatacctgc agttgcttca tctaagatta atagcttcac atcatgagaa agcgcaatcg
    12061 ttaaagctat cttcattcgc atccctcttg aaaaagtttt aattttagtt tgtcgtggta
    12121 actcgaaata tttgattaaa tcaaagaatt tttgactatt ccaagtcgta taaatagatt
    12181 gaaatacttt atcaatatct ttaatagtca atttattagg tactctcaga tcatcaaaca
    12241 caacacctat gtgttcttta tattcgacat catccgcagc atgttcttct ccaaaaaatg
    12301 caatactacc gttatcttta tgtcgattgc ctactagaat attaattaat gtcgatttac
    12361 ctgagccatt tttcccaatt aatccaactg tctcattagt agaaatcgtt aaagatgcat
    12421 cttgcaattt aaaatccgaa tctttgtatt gtttgttcac acgatctacg tttaacaatg
    12481 ttgtcatatc catgctcctc tttgtttaat tttaataaaa acgtcttctc ttcaataaat
    12541 agctaaaggc gttatatatg aaaatagcaa tgataagtag caaactaaag ctttgattaa
    12601 ttcctgtagc aattgaataa gaccctataa aaataatggc tagtatcaat attgataagt
    12661 tgatcgcata tgttttaaat aatgcaatta attctatatg gcgttcacct tcatccaata
    12721 tttccaatcg cttttcagtg taattcttat ctgcaatttt tggcattctg tcatcaaatt
    12781 ttctattaaa cagtgtaaat tgtgtattga aaatagcact agcaaaaaat ggtattaaaa
    12841 agaatagcat tgcatttgct tcagcttgcc ctaccacaaa aattagtaac actacaaagg
    12901 caataattgt ctgtataacg cttaatatac ttccatttaa aacataacga ttcgcaagca
    12961 attcatattg atctgttgca tcgatatcta cctcttcatc tacaagtcgc ttatacttca
    13021 atgcacgtct ttgattcatc aaaacaatgg cttctaccaa tatgataata atcgtcgcaa
    13081 caatcgatat tacaacgaca ttggtatatg tcgcaaacgt caagttctca atacccaatc
    13141 catcaaaatt acctaaaatt ccaccaatga taccccctac aagtccacct agtaaaagat
    13201 atccgatata tcttagtatt ttcatacctc atcctcctca ataataaaaa cagtttccac
    13261 cgtttcattg aagatacgag caatctttat tgcagttaat accgatggca taaagttatt
    13321 tcgctcaatt agcgaaatgg tttgtcttga aacacccgct agtttggcaa gctgcgtttg
    13381 gtttaagcca tctcgtgctc gtaattcttt caatcgatta cgcacatcgc atcaactcct
    13441 taattacact gtaaatgata tttgtctttt tgacaactat atttgtcaaa tatgtacaaa
    13501 aaatatgatt atccagctat gtatgacatt ttgaaacaaa cacctcaacg cctacaagtc
    13561 ataattgttt actttcgtta caccttcccg cataattaac agcattctaa ttttagtatg
    13621 atgcacgcat tttcactaaa tcaaacgatt caaaggagac tattatggca tttacattat
    13681 ctgcaattca acaagcacat caacaattta ctggtgttga ttttccaaaa ctattcaaag
    13741 cttttaaaga tatggggatg acttacaata tcgtcaacat tcaagatggc actgcaacat
    13801 acgtacatca atcagaagat gatatcgtta cgtcatctgt gaaaagtacg attcctgttg
    13861 ctcaaaaatc aaatcaatct atcgttcaag atgtattaac tagacatcaa caaggacaaa
    13921 cagattttga aacattttgt gatgaaatgg ctgaagctgg catttataaa tggcatatcg
    13981 atattcaagc gggcacttgt acttatatcg acttgcaaga ccaagctgtt atttcagaat
    14041 taatccctca ataagttgtg tttatagcaa tattttaatt attatataaa atttgttgat
    14101 aatcattatc gttcggtata aagtaaatac tatatactac ttatgagtga ggttgattat
    14161 catgataact aacactttta ttttaggcat cacaggtcca acaagtcttg tcgtcattag
    14221 cattatcgct ttaattattt ttggtccgaa aaaattacca caatttggtc gtgctatcgg
    14281 ttctacttta aaagaattta aatctgcaac agaagattta gataaagagt ctcacgatac
    14341 acccagtaag gaatcgaagc aacagcgaga gcaatagtac tgatgataca ttaatggttc
    14401 actttagcga actacgccat cggttagtaa aaattttatt gtcgttcgtc attacggtca
    14461 tcgtcgtata tgtttcatca ttttggtgga tgacaccatt cataacgtat attacccgtg
    14521 cacatgtgtc cttacatgca ttttcattca cagaaatgat ccaaatatac gtgatgatta
    14581 tatttttcat cgcgttttgt ctcatttcac cggttatgtt ttatcaattg tgggcgtttg
    14641 ttgcgcctgg tttgcataat aatgagcgcc aatttattta taaatatagc ttttttagcg
    14701 tacttttatt tatttttggt gtcgcttttg cattttatgt tggctttcca atgatcattc
    14761 aattcgcgtt aaaattatca ttaactttaa acatttcgcc tgtcattggc tttaaagcat
    14821 atttgattga acttatacgt tggttattca catttggcat tctatttcaa ttaccaatat
    14881 tattcatagg acttgcaaaa ttcggtctta tagataccac atcattgaag cattatcgaa
    14941 aatatattta tttcgcttgc tttgtattag caagtatcat tgcaccaccc gatttaacat
    15001 taaatatatt actcacattg ccactcatat tgctctttga gttcagtacg ttcatcgtta
    15061 aatttacata ccgaggcaaa ccgccaacac attaatctta taagaattaa tgctgtgatt
    15121 atcgttatac tgcaataccg aatatttcca acatcgcaac aaaaaaacag gcactcggcc
    15181 tgttttttat tttttaaatt taaagattaa gatagcaact accataatat aggcagcttg
    15241 tgctatcaat ggttcataag ttggatagaa tcctaaccag ttaatcgttg ggaatccttc
    15301 aatgacatgt ctcggcatag cacctaataa ttgtaacttt tgaatactta cgccaagcat
    15361 tttgaatccc ataataaaaa taaaaatcga caacactcta aatatataga atataggtat
    15421 caatctgacg ataaatctaa ataataacgc aaagataatt aatataacga tagctaaagc
    15481 aataccaata ataaaatctt ttgtcgctag ctcacctatc atccccatat agaaaatgat
    15541 aacctcaaca ccttcacgca acacagatat taaaccaatc gtcgctaaca ataccaaatt
    15601 accattacta atcgcattgg catacatatt tttaatcata tcattccagc gtttcgcatt
    15661 tgaacgtttg tgcatccaaa caccaacgat aaacattaat atgaccgcaa caatacctaa
    15721 tcccgcttcc atactttcac gaagaatgcc actattacct aaagtttcta caaacgtaat
    15781 tgctaagata atactcagta caagtccggc aattgcacca ccaatcacac ttgcagtacc
    15841 cttcttatct ttgacattac gcgtcatagt agtcaatgtc attacaatta ataacacttc
    15901 tagaccttca cgtaaaaaga taatcatcac atcaacaaag ctataactat ggccaacaac
    15961 ctctttaatt tggttattta aatctactaa accatctttc acatgtgctt tattatgttc
    16021 gtctaataca ctttgataat atggtatttt atcttcaatt ttcgtataca aagcaccgtc
    16081 tttagtttga atttgacctt caacatacgg ccaaatttct ataaaatgtg taagcgcagc
    16141 atcagcatcc gacaattgat tgtcgtcgat agctttaatc gccttctcta actcatcatt
    16201 taattgtgat acatggtatt gatcttttgc agacgtatca cttttcttat cgacatgatc
    16261 aatatttgat ttaaaagttg tccaagcatg tgacactttc gctgtatcta atggcgactt
    16321 atgaattgcg attctaagtt gcagtaatgc gacttcaatt tgtccatatt gatttgcgtc
    16381 ataattacga attaccgttt cattacttgt ccaaatctga ttcaaactat tgttcagcga
    16441 ttctagctct gctttatttt tatctttaat cgcttttgtc attactgcat ctttggcatc
    16501 gacttgctgt tgcaatagtt taattttaga acccgcatct ttactagcca atttctcttc
    16561 ataagcaatt aatgacttcg ttaattgcga aagtgtatct ttttgattat cattcgcttt
    16621 tgcatcttca agctttctca catctgattt gacagcatta ccttcgctat tatcttcaag
    16681 cgataatttc ttaactgcac ttaccacttg ctcaattgct ttctgcttat tgtcattcga
    16741 tatcgaatta ttagaaagtg cagatttcgc atccgttatc acactatata catcactaat
    16801 actttgttgt tttgctgcct gacttttcag taacccaaag ctacacacca tagcagcagt
    16861 tattagcatt gctacaaatt taatcaaata atgtttcacc aaggtatcct cccttactaa
    16921 cacctggtaa tactaaaaat gaagcagaac ctctatgtgt aatatattca tttaatttat
    16981 cattactacc taaattattt tgtatatcga taaattgttt tgtcgctttt tgaaaagcaa
    17041 tgaacaacaa gcctgtttcg aagttacctg tgcggtcatc cgtaccatca acataattaa
    17101 aggctctacg taaaattgac gtatttgctt ctttcgctag tctcgtatgg gcatctttat
    17161 caataatata ctcgccatga ctatctttcg cttttaagtc aatttcatca aactctttcc
    17221 cacctgttaa cggcgcacca ctatgtcgtt tccgaccaaa tgtagcctct tgttcttcca
    17281 gtgcagtacg atcccacgtt tcaatgtgta tttgaatacg tctgacaaca caataagttc
    17341 catgtttcgc ccatccgtca tcaataaaca cataatcttt aagttgatta ctctttctag
    17401 gattaattgt tccatcttta aatgccatta aatttctaga tgtttcctta cctttagcag
    17461 agataaaacc agattgcgcc caacgtacct taacaatatc tcgaaatgga cgaactaaat
    17521 tatgaaccgc atgaaaggat acttgcgaat catttgagca tgcttgaatc ataatatcac
    17581 catcgctgta atcgtctatt aactgatcat tcggaaaatg cggtaaatct ttaaaggcat
    17641 cgggaatttt actagataac ccaattttct tcatcaaaga cttactaatc ccaaaggtaa
    17701 tcgttaactt gcttgcacct aatcctatcg attcaccggt atctactggt ggcattaaag
    17761 gattgttact cggcttgcca actttatcac catccatcat acgatcagcc ataaccgtcc
    17821 actttttaaa cattgcctta attgcatctc tatcttttga cttcaaatct aacgcaacga
    17881 aattgcatgt tttttgcgtg ggtgtggtaa tgcctggttg cactttacca taaaattcat
    17941 acgcatcttt ttccggatct tctggtgtat tgaacattga tttgaaagac cacatgctac
    18001 caacaccact tgcgccaatt gcaacaccgg caccgccaat acctaacatt ttcaaaaatg
    18061 tacgtcttga aaattgcgta ctatcgttaa cttgttcata atttgtcatt cagtaatcac
    18121 agccatttta cttagtggct ctcctaatgc attaacagca tccgcaaatg ctttacggtc
    18181 tttcttagat actttttcaa atgactcata accgccgttg ttatctttat atttatccaa
    18241 taattgattc actttatcga agttcatttg aatatcatca cttaattttt tatctttttt
    18301 ctctaaaata ggtttaaata agtcataaat tttttgtgca ccttcaatgt tcgctttaaa
    18361 atcatataaa tctgtatgtg aataaatttc ttcttcacct gtgattttag aagttgcaac
    18421 ttcatttaat aggtcaacag aaccttgtaa cattaatttt ggtgtgatat ctaatgtatc
    18481 agctttggca tgcaattctt ttgcatcttt caataattgt tgtgcatctt ttttagtcac
    18541 atcatcaatt ttcttatctt cgtataatgc tttttcaatc ttatgatatc ctgaccattc
    18601 tttttctttt ttctcttctt tcatatctgc aagacgtgca tcaattttag gatctaaatc
    18661 tccaaatgct tctgcaactg gttcagagcg ttcataatac atacgaactt ttggatataa
    18721 cgcttttgct tgggccatat cattattttc aatcgcttta acaaattttt ctgtaccttt
    18781 taaaaattca tttaactgtt catcagtata tttatcatat gcttttgttg cttgttttaa
    18841 ttctgcttta acaccatcat cttttttcga tgtctttgaa tcatccttct tactatcgtc
    18901 gttcccacat gcagcaatta ataacgttga tgctaataat agcgttgtta actttttcat
    18961 tttgacacct cattatagaa attctattat ccccatagac atgccctcat tcgtactatc
    19021 tcacactccc taaatgccaa gataatacgt ggctcttaaa atgatgattt taaggcatcc
    19081 tcccatgaac attatctagt tgataataat tatcaaaatc attatattgt tataaaataa
    19141 tactgtcaac aaccttttat tttaaaaaat taaatgaaag agcgatgcca ttttaaatgt
    19201 catcactctt ttgtaagtct aatttgtcat ttttggtcgt attctgattt taataaacta
    19261 tagatgtaac tcgatgaata aataccattt agtaattcat tgtcacgtaa cataccttct
    19321 ctagtaaatc ccagcctttc aggaatagct cggctttttt cattattaac tgcggcacta
    19381 atctcaattc ggtttaagtc aagctcttcg aaacaatact ttatcaatgc ctcaactgct
    19441 tgtgtcataa tcccatgacc ctcaaattgt ttatctaaat aatatcctaa tgaagttttt
    19501 ctgtgcatgc gattaatttc atgtaaaccg atgacaccaa ctagcgttcc ttcataccaa
    19561 atgccacact gaaatccatt accatcagaa aattgcaaaa gtcctctttt aataaacgca
    19621 cgcgtatctg atggttgctc agttgcatct acccaaggta accattccct aagtgaattt
    19681 cttgaacgat tgactaaatt gaaaagcgct tctgtgtcat gggcttctaa aatttttaat
    19741 attatttgtt cattcacttt cattccaaac ataccatcac accctcattt atttttcata
    19801 taattttgta aattatcata ataataacat attaatatta cgccttgtat cttaaatttg
    19861 ttgtccttcc ccaacttgca ttgcttgtag aatttctttt cgaaattctc tttgttgggg
    19921 ccccgccaat aagcgttgta gtgcctatta ctttgattga tgtccaagtt tcctatcaca
    19981 caacttaatt taatctctac tttattaacc attccaaatt tatatttaaa aatacgacaa
    20041 aacagtgcta aaatgctatt aaaggaggca ctgctcatgg catcattgaa aaataagcat
    20101 tttatgattg gactaacatt aacatttata gtcgcattat ttagcttttt agctgctaaa
    20161 ttgccaatct tagataaggt tggcgcatta actatcgcga tattaatcgc tattctatat
    20221 cgtcacttta gaggatatcc tgaacaatat agctcaggta ttacgttttc atctaaatat
    20281 ttattaagat ttgcaatcat cctatatgga cttaaactca atatattcga tattatcggt
    20341 caaggtagta gactattagc catcgatgta ggtgtcgtta tttttagtat tgtaatgatg
    20401 ctctttgtca acaaactgtt gcatggtgac aaaaacatcg cattattact aggtgtaggt
    20461 acaggcgttt gtggtgctgc cgctattgct gcagtcgctc caatattcaa atcacgtgaa
    20521 aaggatacag ctattagtat cggtatcatc gcattgattg gtacgatatt ttcacttata
    20581 tatacagcta tctatgctat cttttcaatg acgacaaatg tttatggcgc ttggtctggc
    20641 gttagtcttc atgaaattgc acacgttgtc ctagctggtg gctttggtgg tagtgatgca
    20701 cttaaaattg cacttcttgg taaacttggc agagtattct tactgattcc attaaccatc
    20761 gtacttattt taattatgcg tttccgttca tcagaatcat ctagcaatgg tcgaataagc
    20821 attccatact ttttaattgg atttgtcatt atggccttag tgaatacata tgtaactatt
    20881 ccgtcagcat tgctaaatat tttaaatact gtatcaacaa tttgcttatt aatggccatg
    20941 gttgctttag ggttaaatgt tgcatttaaa gatcttaaaa atcgcgcact taaaccgctt
    21001 atgacaatca ttattacctc aatatgcttg tcatcacttg cctttatcgt cgttcattgg
    21061 ctatatagtt aatatgaaaa gacgctcaaa cacttgcaaa tttagtgtgc cgagcgtctt
    21121 atttttatac tttaaaagtc gtattggttg ttttcttctt caaacacttg ttgttgtact
    21181 ttggcagttc gcattgtgga ttgtgccaac gcttttaacc gaaatacaac atcatcattg
    21241 acaatttgtt gatttgaaaa atctttctct tcaataaata catacgtttg catcgtatgt
    21301 gctttcatgt aactcaatat tggttttaaa tgcatttcag gaattaaata atgtttacta
    21361 gaacctgctg tcgctacaag tcctattacc ttgtcacgaa acgcattgac tggaagtaga
    21421 tcaaacacat ttttcaaagc accagggatg gaagcttgaa aaattggaaa accaataaaa
    21481 atcacatcag cctgcattaa cgacgtcgtt aatttatata catctcctgt agtatctaga
    21541 taattctttc caacgctaaa ttctaattca agttctcgta aatccatcaa ctctatttga
    21601 tgtccctcat tgatgacttc tagttcattt tttaaatcat ccatagcaat tctcgtttta
    21661 gaacctactg tggaacctga caataataca atattcatga tgacgcccct cctattttga
    21721 taaatgcttt ttaatcgccg gtataatgtc gttgccaatt aactctatat tcttcataac
    21781 attttcaaat ggcacaccgc caaaatcaag ctgtgccata aaacgttgat gaccatacaa
    21841 ctcgtgttga tacaatatct tttcaataat ttgttgcggg cttccaacca ttagcgcttc
    21901 tctataatct ggtgaattag caaattgctg tttcggataa ccaacgccac gaataaatga
    21961 catccctgta ttcaaatgtg gataaaattc tctcatagca tcctgagttg tttcagctgt
    22021 ataaaacaga ctcgctatac ttactggtaa agacttaggc gaagcatcga aacctgcttc
    22081 agttgctgct tgacgataag catctataga acctttaaag ttcattgctg ggccaccaag
    22141 ggttgtaatc atcattggca caccttgttt tcccgcttta atagcacttg caggtggacc
    22201 accaacagca cgccatattg gcaatgtatt atcgattggt cttgggaata ttttcatatt
    22261 tctaagttct ggacgatatt ttccagacca agtaatacgc tcagttttat ttaactctaa
    22321 aagtaaacct aatttttctt caaacaattc atcatagtct tttaaatcat agccaaacaa
    22381 gtcaaaaata cctgttcttg atgctctgcc agctacaatt tcggctctac catgagaaat
    22441 caaatctaat gtcgcgaagt cttcaaatac tcttacagga tctgttgcac taataatcgt
    22501 tgaagaactt gaaactttga tatgctttgt cgcttgggca attgcaccta acacaaccgt
    22561 aggtgcctgt gttgtaaaat gctcctgatg actttcacca actgcaaaaa catcaatacc
    22621 tgcttcatct gctaatttac ttgcttcaat aatttcatta atacgttgct cataactaac
    22681 tttttcacct ttcaatggat tcaataaatg atcacctaag gaatacaaac caaactcaag
    22741 aggtgtattt ttattcattt ctaattttgc cattaaacat tctcctttat atttcgtgta
    22801 acttactttc aatttgttca cgttctgctt ctaaaaagtc aggcaagttc aattgacttc
    22861 ctaattgttc aagaggtgta tcaatagtaa atccaggcgc ttcagtcgca aattcataca
    22921 taattgaatt atggcgatag tataatgatt taaagaaata gcgatcaatt atacctgaat
    22981 tactttgtgt ttgttgttgt aatttcttgt aaattgcatc taagtcactc atttgtggtg
    23041 tattaattgc aatatgatgg atataacctc gtccaggtct ttcacgttgt ccctgttgct
    23101 caataactac aaaatccgaa tataaacctt gtggtgctaa tgtcagcaca tcgaatgatt
    23161 tattatctct tttgcgataa cctaagacat tttccaaaaa ttctaccgta cgcgctgcat
    23221 ttctaactct taattcgact ggccctattc ctaaaatttg atatgcttga ggtatttcac
    23281 tataaacgtt atgttgccat tgatgtggta cttcaaaact atcattaaca agcaatatga
    23341 tttctaagtc gtcttcatct ttaaagacaa caccttcttg tccaagatag tacaaacgtt
    23401 cagacgtaat accattatta tcaaggcgtt tttcaaaata atgaagtgcc gcttgatttg
    23461 gtactaataa agaaaatcga taaatcgttt cagtacctgg ctgcttatga cccgcattgg
    23521 gaatttcaaa aaagcttaaa attgttccgg ctgtacctac ttcgtcccca taaaacaaat
    23581 gatacattga aggattgtct tgattaaccg acttttcaac taatcgtaat ccaaggacat
    23641 ttgtgtaaaa atccttatta cgttttgcat cttttgtata catggatatg tgatgatgcc
    23701 ctactatatt catactatcc ctcctattag tataaatttt ataccaactt acataaaaaa
    23761 gatagttata tgccatttac cccttttctg caaataaaaa tatgtttgta ctcattcgta
    23821 gatacacagc tagtatgtat aacgacataa taattgcata attataaatt tatgtgtagc
    23881 aatttatttt ttctaaatgt ttataaaata cttcaataac taaatatgca agatttcaca
    23941 aaagtatatt gacaacgctt tcatacatgt ctaatataag tttatcgcac aaaatgcata
    24001 taaatacata ttaaggagga ttttatgaat tttcttaaac ctgcaaagca tattaagcct
    24061 ttgccagaaa atcagataga tgatacctat aaacgattac gtctccaagt atttcttggt
    24121 attttcatcg gttacgctgg gtactattta ttacgtaaaa acttttcatt agcgatgcca
    24181 gcattgcaag agcaaggttt tacaaaagcg gaactaggtt ttgcactttc tgctgtttcc
    24241 atcgcatatg gatttagtaa gttctttatg ggtactgtaa gtgatcggag caatgctcgg
    24301 atattcttag ttcttggatt agcactcact gctatcgtca atttgttaat gggatttgta
    24361 ccgttcttta catcaggtat cggtattatg tttgtcctat tattcttaaa tggatggttt
    24421 caaggtatgg gctggccacc ttcaggccgt gttctcgttc actggtttag tgtaagtgaa
    24481 cgcggaagta agactgccct ttggaacgtt gcgcataatg ttggtggagg tattatggca
    24541 cctattgctg cttggggtat tacaacaaca gcatttatca actttggtta tttaaaaggg
    24601 ttcgaaggtg tattcattta ccctgcactc ttagcactta tcattgccgc aatttcatac
    24661 gtattgatta gagacacacc tcaatctcaa ggtttacctc caatcgaaat ttataaaaac
    24721 gactttgcta caagcgataa gaaaacatta gaaacagaat taactacaaa agaaatttta
    24781 tttaaatatg tactaaacaa taaatgggta tgggcaattg cctttgcaaa tatatttgtt
    24841 tatttcgtgc gttatggtgt acttgattgg gcgccagtct acttaagtga agaaaagcat
    24901 ttcgacttaa aagcatcagg ttgggcatac ttcttatacg aatgggctgg aattcctggt
    24961 acattattat gtggttacat ttctgataaa ttattcaaag gtcgtcgagg acctgcaggt
    25021 ttcttcttta tgttaggtgt cacagtattt gtattaattt attggttaaa tcctccaggc
    25081 aatgcttggt tagacaatgt ctcattaatt gccattggtt tcttaatata tggaccagtt
    25141 atgttaattg gtttacaagc attagattat gtacctaaaa aagcagctgg cacagcagct
    25201 ggattaacag gattatttgg ttatctgttt ggtgctgtaa tggccaacat cgtcttaggt
    25261 gctgtagttg ataaattcgg atgggatgtc ggttttattt tattaacagc aatcagtgtg
    25321 tttgcaatgt tgagctttat cctcacttgg aataaagtag gacaagaaac cgttcatcat
    25381 taaatgataa aaaataaagt catatggtta tcttatcgaa agatgatata ttcatctctt
    25441 ataagttcaa ccatatgact ttttattagt attcaaaaaa atatttacat tgccactttt
    25501 gtgtttgccc tgctgtttta ttcaattgat tacaccactt aggataaact ctaaaagcca
    25561 tcttaccttt atgttcaaat gtagttaaga tacctttttc taccaataat tccctaggaa
    25621 ataaaaaata accgcttaat tcttcatcaa taactataat cattaagtaa tctgcaaatg
    25681 cctcttttga gtatggttga ttacaattgt tctcgtcttt tgtccaacat gtaacaaaat
    25741 accctgcttt agtcggtgtt ttcttagcta acctactttg atatgtttcc tctttaaagc
    25801 taaatgttaa tgcttcgtaa tcttgattat atttttcttc agttaaatct ttaacttctg
    25861 gttcttcact aaaaatatat ttcaacagta ttttagattt atacatatgt cattctcact
    25921 ttattttttt cataatcgta tcatatattt attttttatt cgaaaaatac acttccgacc
    25981 gaaatatact cagccagaag tgtacgaaat atttaacctt ctattaattc agatgtatca
    26041 actgtcaaac gatcacgtaa taaatagacg attaacattg ctgccaaagc acaaagtgac
    26101 tcagcaatta ataatgacca tatgacacct gtgaggccga acaaagcatt cataataaat
    26161 aatacaggaa tgataatcgc accttgtaaa atggccataa ttgtagcacc acgaccttgc
    26221 ccagtcgctt gaagcatacc agtaaacaag aaacctatac catttaataa caatgatgtc
    26281 attgttactt tcaaaataaa tgtcgccatc tcaacgatgt cttgatcagt agtaaatagt
    26341 ccgaccatat gatgtccaat tgtaaataca gcaatcatac atacaacaaa gataacaccg
    26401 atagacatga taactgcttt gataacgtct ttcatacgac ctttatttgc cataaagtta
    26461 tatgcaatta gtggtacaac accttcacat aatcccatga taataagttc tggaaattgc
    26521 acaagtctaa atgagatacc ataacttgca atcgcgaagt ttccataatg tgctaaaaat
    26581 aaatttaaaa ctaatcctgt gaatcccatt aagatactca ttaaaaatgc aggaataccg
    26641 attttaaaga tttcagaaag catttcttta ttaggtttcg caagtttaat attaactgac
    26701 acaacgtcac tatttttcat aaaataaacg ataaagaaca gagcagcagc aacattactg
    26761 attgcagtac ccaaagctgc accaacaacg tttaaatcaa aaccaaaaat taaaattgga
    26821 tctaaaataa tatttaagcc tacactagct aacataccaa tcatagaaat cattggtgct
    26881 ccaattgcac gtgcaaattg ttctaatatg aagaacaaaa ttacaaaagg tgcacttaaa
    26941 aacattactt tcaaataatt acttgttaaa gctaacgttt cacctctcgc ccctaaaatt
    27001 gctgcgattt gatcactgaa tggtaaagta actaaaatca cgataagtcc tagtgcaata
    27061 ccaccataaa tagagaaact acttacaaat ttactcttac tatagtcttt cgcacctaat
    27121 aaacgtgaaa tataagttcc tgcaccaacg ccaaataaat tacctaaccc cattaagata
    27181 gcaaatactg gcagtgttag agagatagca gaaatcatgt ggctatcttc taaaaatcct
    27241 ataaagtaaa tatttaatat gccataaata acgcttaata aagtccctat catcattggc
    27301 aatgagaaat gcatcatcgc tttaaatact ggcgatttct caaaataata taattgttcg
    27361 tctttcattg ttcaatactc cttgtctttt ccaataatta gcttactaac aattagatat
    27421 ctaactataa tattaaagac aaagtgactg atttctacca gtcacactta tcatttattg
    27481 taaactagat aacattttag ttaagtttgc tttcatttgt tcattttctt cttcagataa
    27541 ctgcgatacg agtgtttgtt ccatttcatc aaatatcgaa gtgaatgctt ctacgagttt
    27601 aatcccagag gtagtcagcc ctatattctt tcttctcgta tcttgtgcat cgacatagcg
    27661 atagatcagc tttttacgtt caaggttcct taataaatta ctgacagttg gacctgttcg
    27721 ttgtaatgct ttagcaatat cattttgtgt cagtccatct tgttgatgtg cataaagata
    27781 acctaacgta tgaccttgct catttgtaat atcaaattgc tctaactttt gatcagcctt
    27841 ttgtttcatc tcatgactaa tcattctaaa taaatacgaa taagtgaatt ccatttattg
    27901 caccaccatt tcgttagatg tctaactaaa tatactgcga catttttaaa ttgtcaacat
    27961 gaatatctaa tttattttaa aagttcctat aaaatcttgc gccataaaca ttttgatatg
    28021 caatttttcg gattaaaata tgaaagagat tgcatatcta cgtttttaag aaatcgctta
    28081 caattacaaa taggaggata ttacgttgtt aagttataga caaatcgaaa ttttgtatct
    28141 tttaattcat gagcaaacgt ttattccaat caataccatt gctaatcaac ttggtgtatc
    28201 acctcgtacc attcaatatg atatagcata tattgagcaa tatgctgaaa cttatcatta
    28261 ccaagtcagc cgtaataaag ctgcaggcat caaagtaaca acagctcacg cgacactatt
    28321 aaatgaatta gagcacaatc taacaaatca aattcatttt tcaaaagatg aacgactcac
    28381 acatatcgct ttaaagttat tcgaaacaac cgatcctgtt tcaacaaagc aacttgcgca
    28441 agatgttaat gtttcgcgtc ggacaattgc agatgatatt aaaatgattc aagcccaact
    28501 agatcaatac catttaaagt taaattatgt ccacaataaa ggattcaata ttattggtga
    28561 ggaagatcat tatcgtaaag cgtatgcaca ctttatacat caatatatga aacaagctgc
    28621 accttttata gaggcggata tctttaattc agaatcaatc gcattggttc gccgtgccat
    28681 tattaagaca ttaaatagtg aaaattatca tttagttcag tcggctatcg atggcttaat
    28741 ctatcatata ctcattgcca ttcagcgttt aaatgaaaat ttttcgttcg atatacctat
    28801 caatgaaatt gataaatggc gacatactaa tcagtatgct attgcttcaa aaatgataga
    28861 aaacttagaa cgcagttgta atgtcacatt tccagagtct gaaattatct ttatcacgtt
    28921 acatttacta ggctctaaaa tgactgaaca tacagcatct tcaattacct ttgaatacca
    28981 tgatttatcg caaaatatac atgaattgat cacttgtgtt agccaagaat taggcattga
    29041 tatgtcaaaa gacaacaagt tacataccag tctgatcaca catatcaaac cagctataca
    29101 tcgtattaaa tacgatatgc tacaacctaa tcctttgagg caagaagtta tgcgtcgcta
    29161 tcctcaaatc attgaagccg ttagcaagca tattagtcca attgaacaag atgctgctat
    29221 tcgcttcaac gaagatgaat taacatacat tacaattcac ttcgcatcaa gtatagagcg
    29281 tgttgcaaca cataaacaat caatgattaa ggttgtctta ctatgtggtt ctggtatagg
    29341 cacgtcacaa cttttaaaat caaaactaaa tcacctgtat cctgagtttc acatttggga
    29401 tgcctattcc atttatcaat tggaagaaag tcgattattg caagataaca ttgattatgt
    29461 catttcaaca gtaccttgtg aaatatcagc tgtaccagtt attcatgtcg atccatttat
    29521 caatcaacaa tctcgtcaaa aattgaatca aattatcaat gactcaagag aacaacgagt
    29581 catgaaaatg gcaactgatg gcaagtcact cgcagattta ttgcctgaac atcgcatcat
    29641 tataaataaa caaccattat caattgaatc cgcaattgca gtggctgtgc aacctttaat
    29701 caatgatggc attgtctatt caaattatac agctgcaatt ttaaaacaat ttgaacaatt
    29761 cgggtcatat atggtcatta gtccacatat tgcacttatt cacgctggta ctgattatgt
    29821 acagaatggt gtaggtttcg cactaacata tttcactgaa gggattatct ttggtagtaa
    29881 agctaacgat cccgttcacc ttgtaattac attagcaacg gaccacccca atgcacattt
    29941 aaaggcattg ggacagttaa gcgaatgctt aagcaacgac ttatatcgac aagatttctt
    30001 agatgggaat atttttaaaa ttaaacaaca cattgcttta actatgacaa aggaggctta
    30061 ataacgtgtc attagacatt ttgtcaacaa cacgcatcat tgtaaaagaa caagtaaatg
    30121 attggactga agctataact atagcttctc agccattact acaagaacaa attattgaac
    30181 aaggctatgt tcaagcaatg attgatagcg ttaatgaact tggaccttat atcgttatcg
    30241 cacctgaaat tgcaattgca catgcaagac cgaacaatga cgtacatcaa gttggtttaa
    30301 gtctattaaa gttgaatcaa catgtggcat tttgtgatga agatcactac gcatctctca
    30361 tttttgtatt gagtgccatc gacaatcatt cacacttatc tgtattacaa aatttagcaa
    30421 ccgtactggg cgataaccaa acagtccagc aactattaac tgcaacaaat gcacaagaca
    30481 ttaaaaacat tttaaaggag catgattaat atgaaaattt tagtagtatg tggccacggt
    30541 ttaggaagta gttttatggt agaaatgaac gcacaagaag cacttaggca acttaatgca
    30601 ccatctgata tcgaagttga acatagtgac attatgacag caagtccaga gatggctgac
    30661 ttgtttattt gtggtagaga tttagctgaa aatgccgaac gtctagggga tgtcttagtt
    30721 cttgataata ttttagataa agctgaatta caacaaaagc tctcagaaaa attacaacaa
    30781 cttaacatga tttaaaggag gtacgaccta tgcaagcaat ccttaatttt atagtcgata
    30841 ttttaagtca accagccatt cttgttgcac tgattgcctt tataggttta atcgttcaga
    30901 aaaaacctgc cgcaacgatc acttcaggaa ccattaaaac gatattaggc ttcttaattt
    30961 taagtgcagg tgctgatgtc gtcgttcgat ctcttgaacc attcggcaaa atattccaac
    31021 acgcatttgg tgtgcaaggt atcgtaccta acaacgaagc tatcgtctca ctagccttaa
    31081 aagattttgg aacaacagct gcactcatca tggtctgtgg catgattgtt aatattttaa
    31141 ttgcccgctt cactaattta aaatatatct ttttaacagg tcatcataca ttttacatgg
    31201 ctgcgttttt agcaatcatt ttaacagtca gtcatattaa aggctggcta acgattgtta
    31261 tcggcgcact cgtattagga ttaatcatgg cagtattacc tgcattactc caacctacga
    31321 tgcgaaaaat tacagggaat gaccaagtag ctttaggtca ttttggctca atcagttact
    31381 ttgccgcagg tgctgtaggt caattattca aaggtaagtc taaatcaacg gaagagatta
    31441 aatttccaaa aggcttaagt ttcttacgag aaagtacaat tagtatctcg attacgatgg
    31501 cattacttta cttcatcgca tgcttatttg cgggcgttag ttatgtacac gagtctatta
    31561 gtaatggtca aaactttatt gtcttttcat taattcaagg tgtgacattt gctgctggtg
    31621 tatttattat tttaacgggc gttcgcttaa tcttagctga aatcgtccca gcatttaaag
    31681 gaatttctga aaagcttgta ccaaattcta aacctgcatt agactgccct attgtgttcc
    31741 cttatgcaca aaatgcagta ttaattggat tctttgtcag ctttattaca ggtgtcatcg
    31801 gtatgtttat cttattctta tttggtggcg tcgtcatttt acctggcgta gttgcacact
    31861 tcttcttagg tgcaacggct gctgtattcg gtaatgcaag aggcggtatt aaaggtgctg
    31921 ttgctggcgc cgctctaaat ggtatcctaa taacgttttt accattatta ttcttgccat
    31981 ttttaggcga attaggtggt gctgcaacaa cattctcaga tacagacttt ttaactgtcg
    32041 gtatcgtgtt cggtaacgca gtaaaatata tgggattatt tggtgcgatt ctatttatta
    32101 ttatcgtagg tgcgacagca attttattaa aaggccgtca aaagccacag caatagtgtt
    32161 aacgtaaata tataaaacac cgtcacatat tgagtgaatg cctcttttat caagaggaaa
    32221 gccacttact tatggacggt gttttgtatt atattaaatg atacttagcc atactatcga
    32281 cagctgctaa aattgcttct ttttgtgtcg caatcggttc ccaaccaaga agtatttttg
    32341 cacgttcgtt acttacattt cgattcatat ctaataaaag tttaccttct ttagcttgat
    32401 gattaaattt agcacctaga ctcaaaacaa agtctggtaa ttttttagta gaaacttttt
    32461 gagctatttc aggtctcttt tctttaatta attttgcaat ttccaacaaa ttaatttgtc
    32521 catcagctgt agcaataaat cgcttgccat tagcctgttc atttgtcatt gccaaaatgt
    32581 gtagttcagc tacgtctctc acatcaacaa cattcaatgg aatttgcggt acacgtttca
    32641 ttgaaccatt caataaattt tctaataaat gaaagcttcc tgaaacgtgt gcatctaatg
    32701 atggcccaaa aattgcaact ggattgattg tggcaaattc tactgttgta ttttcattct
    32761 caacaaaatc ccacgctgcc ttttcagcta acaattttga tttttcatat actgataagc
    32821 ctggttcatc ttcatttgtc caatgacttt catttgtgat tgaattttta tctttattgc
    32881 taaaaccaac tgcaccaaag tttgcagtca tcaccacacg cttcacgcca gcatgttctg
    32941 cagctcttaa aatacgttgt atgccttcaa tggcaggctt cgccatcact tctgcatcgt
    33001 ctgttttacc gaaaaacacc ggagatgcta cactcaagac atatttacaa tctttcattg
    33061 cttcatccca atgttcatct tgtgataaat ccgcttcgac aaacattaat cgctctgtgg
    33121 aaatgccatt gtcttgcatt gttttaatta ctttatcagc tttacttaaa tcacgtatcg
    33181 ttgtttgtac ctcataacct tgttctaata atcgtgaaat aattcgcatg ccaacaaacc
    33241 ctgtaccacc ggttactaat actttattat tcatcacata atctcctctt tgtgtctatt
    33301 gattactcat tatagcagtc attatatttt gacgtatata aatagacttt agtagctagt
    33361 atttaatctt acttatataa atgaaaatga atttattcct ttaaaaacta tcattaacca
    33421 ttttcaaata cccaatcatt tagaaacaca aacgctataa atatgatgtc tttgctaatt
    33481 aactaaattc attttcaatc gtatgttcaa attacataac tacatagtca ccattttaaa
    33541 agcaaataca catattcatt gagggtgcga cattaacgcc taaacaatat taacttaaaa
    33601 tcatctcaac caatgatcca accgtaacat taccaaaata ataaacgatg tctaattgct
    33661 ttaactgatg cgttaaatct tctcttgtca tttttgttcc ttgtaatgct tcttccacat
    33721 cttttatatc accttgtcca aagaaatcac caaaaattct acaatctgca atacgatttt
    33781 gttcaacaga aatggtaata tctacagtac ctgatgataa tctttcactt cgattatatt
    33841 catatttggg tgacttgcca taattccaat cccaattttt atacttttta tcaactaatt
    33901 catcaatcgc ttcccaatct gcatccgtta attcgtatcg ttttgcctct ttaatgtcat
    33961 cgattcccaa aatctgtgtc accattaaat ttttaaattc ttcaatggtt ataccacgat
    34021 acttttcatc cagtgcttct ctaagatgac caacacgtgc gcgcacagat ttaatccctt
    34081 tcgatgcaat ctttttgcga ttaggcttta acactttatc cattgcttca taattaacat
    34141 caagtaatag cgaataaccg ccataaatac gattgttcat taatgtcatt gcggcgcctg
    34201 acactttttt accattcaat gttaaatcat ttctaccgct ttgtatcaca tctgttgcac
    34261 ctaatgtatg caacgccttt atagctggct gataaaatcg ttgaaaatca ccataaattg
    34321 aagtgtcttg ttctaaaata caacacatat taacggcacc tttatcaaca tacacagcgc
    34381 caccaccagt atctcggcga actacttgaa tactgtgttt atctatataa tcttgattca
    34441 cttctatagc agtattttga aaacgtccaa tttccacttt tggatcacaa taataaggga
    34501 ataaaatatc ttcatctaaa aagatatgct gattaacata aacttgcata gcgagtgcaa
    34561 tcgcaccatc agtaatatat tctccattcc taatcggttc tattaagtac atcttttgtc
    34621 tctccaatgt tgttattttg cgttgtgcta tcgttccgga gtgctgttgt aatcaatgtt
    34681 gagatatcct cagttaaatg tatggtacgt tcttgaattg aattcggaat gcgatatgcc
    34741 tttttattca tcgtcatata aagtgcattt tcatttttac gtgtcatacg ctgaaaagga
    34801 tgcttcacaa attgtggtgt agtataacca attccaattt ccaaatacaa cactttatca
    34861 tcttgatgtt gctctagaaa agcattataa cgatgtagtt gcgcatgaaa ttcagcatct
    34921 tcaaccatcc caacttccgc tttacgttta ttcacttcca ttggggcatc acattttgga
    34981 catcttggaa tcatctccca aggtataagc atatcttgtt gcgcaacaac cattttacga
    35041 attaaatcat cattgcgata cgtttgagca tgacaatgct gactacactg ttgcagtata
    35101 tactcccctt gtatatgaaa tacatgagtc atatcataat cagctacatc gaaagcatta
    35161 tctgcattcg tagttataat gtggtactgt ttaccttcca ccaaggattt taaagcgagg
    35221 taagactgac ctacaggttg atctaaatag tttaatgtaa taaaacgact ctcaaatgcc
    35281 caatactctt gccaactgcc ataaggatgt aaactcgctt gcaacatatc aaagaagcga
    35341 tatttttcaa taaaatctgg gaaattttcc gtaaaacgct ctcctacata tgtaaatccg
    35401 tcagatgcag acatgcctgc accaattcca atcactatcg catctgcttc atcaatcgca
    35461 gtacgcaata cttcagcctg ctttgtcttg tcatccatta acagagacat tgcattccac
    35521 ttactactct gcatcacggt taaatgcctc cttatacagt tgtaaatcct tatctgtaaa
    35581 tacattgaac acgactttca atgttgaatt tgtttctttg agatagcttt ctactgttcg
    35641 aacagcaatt tctgctgctt catcttgagg aaaagcaaat acacctgtag atatacagca
    35701 aaaagcgaca tgatttaaac tatgttgatc agccaattta agacagctaa gataacattt
    35761 agctaacaag tcctgattca tctttgaaac aggcaatcga cgtatttgcg gaccaaccgt
    35821 atgaattata tactttgctg gcaaattata tccacgtgtt attttggctt tacctacacc
    35881 ttcattgcgc ccttgttgtc gaatgatctc tgcacaatca agtcgaactt gaacacccgc
    35941 ttttgtatga ataatattat caatgcagtc atgattagct tgcatacatc ctagaaaacg
    36001 actatttgca gcattaacaa tagcatcgat ttttaacgtc gtgatatcac cttgccaaac
    36061 aaagatattg tcacctttta tcggcttcaa atccttcaaa tccgtaacat gttgacgatt
    36121 caaatcactt aacatagcat cttgtacagc taaatattca tcacttacag gtaatgccgg
    36181 tctgacattt gctaagcctc gatataattc ccacaaatct tcaaatgaag atggcattac
    36241 caatacgtca ttgtcatttc tctctcgatg catatcattg attaaatatt caagtctcgc
    36301 tttatttgat tttaacgttt ccatttaagc ctccggtaat gctaggaatg cttctttatc
    36361 gacatcatcc aatttgaaca accaattttc ttctggtttt tcagagttta aaattgtcgg
    36421 ttcttcttcc gcttttgtat ttcgctcaat aatcgttcct gacaatggcg tttgcacatc
    36481 aatgaccgtt ttcgatgctt cgatactcac aatttcatca tccactttaa cttcatctgg
    36541 acttacgaat tcaacataac ctactgtccc aatatcatct tgcaattcag gtgtcatact
    36601 aaacacatac aaatctccta ctttttctac ccataaataa ttggctaact ttttcatctt
    36661 atcttcatcc tttctcttct atttaaaatg ctacaaataa atttccgcga gtaattttaa
    36721 tgttttacaa cgctgttcaa tacctggaat aagcggtgct actaacacct catcaacttc
    36781 aaacgtagca atgaaatcat ctaattgtgc tttaaccttt tcttgtgtac ctgcaatgat
    36841 gcgtgcttga tgtgcttgaa tcatctcttt gtctcgatca ttaagcttat acttttgtgc
    36901 tgtgtctact gaaggaaaat cttcaaattc tgcaaattgt aatttaccta ataaccaaac
    36961 atctaaggca tgttgtaatg ctgctacttc cgcttcgtta tcagctacaa tgacaaatac
    37021 agatgccatc acctttgcgt ccattttaat cgttgatgct tggaaatgtt ttttgtaaat
    37081 atcaatgtta tccttcgctg catgtatcgc atttatatct ggtagcaaaa atgttccaac
    37141 agaaagccct atacctagtt cggcagctat tttggcagat gttgcgctac tacttaataa
    37201 ccacatttct ggaaaatgat caatgtgtgg ttggacacct aacgtatgcg cacttggttt
    37261 atcctttatt gtaagataat cacgtaataa cgaaatcgat tcatcgtaac tatcatatgt
    37321 aggatttatt ccatctaaag cttgctttac catagtagta cctggattat taccaatacc
    37381 taaatcaata cgatttggat ataacgctgc catcattcta aaatgctcag caattttata
    37441 aggtcgatag tgcggcagca tcacaccacc agagccaact cgtatgtgat ttgtctgcgc
    37501 caatgtatgc atcatcaaaa gttctggact actacacgca aacgctggta cattatgatg
    37561 ttccgtaaac caaattcgct taaagccaag tcgatctgct aattttgcaa gtgtcactga
    37621 atcttgcaat gccttttgtg catccttacc ttcatctatt aaggcatagt ctaatacgct
    37681 taatttaacc aatccgtcat ctccaaactt atcctgtcat gtcaaaccga cataacattt
    37741 tagcgttctt aataccattc cctcttcata tacccacgta tatgataacg tttttcaatt
    37801 aacttttata tcttttcgcc tttatttttc tttttcatat tcatattttc agacctacaa
    37861 atgccattca ttagtttaat tttaaaaata ctttccatgt accaattcta ttctatcaag
    37921 actgttcatc attgattatt cattctttcc atggtacttt gaaaacaata ttgaatagaa
    37981 aaggaatcat agtatgtacc gatacaaacc acttttacaa tcaatccact tacctaatgg
    38041 catcaaaatt tcgaatcgct ttgtattatc accaatgacc gtaaacacat caacaaaaga
    38101 aggctatatt acaaaagaag acttggctta tgctgcacgt cgctctaatt cagctggaat
    38161 gcaagtcacc ggcgctgctt acattgaacc ttatggtcaa ttgtttgaat atggctttaa
    38221 tattgatcat gatgcttgta tccctggatt aactaacatg gcatctacga tgaaacaaca
    38281 tggtagcctc gctattatcc agctagcgca tgctggtcgt ttttcaaatc aagccatttt
    38341 aaattttgga aaggtgtatg gaccaagccc tatgacattg cattcaccga ttgaacacga
    38401 tgttatcgcc atgtctcatg agaaaatcaa tagcatcatt cagcagtatc gcgatgctac
    38461 attacgcgcg attaaagcag gttttgatgg tgtggaaatt tcgattgcac aacgtttgct
    38521 gattcaaaca tttttctcaa ctttttcgaa taaacgtaca gaccattacg gtgccgactc
    38581 attaaaaaat cgtgcaagat tatgtttaga agttatgcgt gcggtacaag aagtgattga
    38641 taaagaagca cctgacaatt ttattttagg atttcgagca acgccagaag aaaccagagg
    38701 cagtgattta ggatatacca ttgacgagtt caaccaatta atcgattggg tcatggacgt
    38761 ttcaaacatc caatacttag caatcgctag ctggggtcga catatttatc aaaatacgtc
    38821 tcgtacacca ggtgatcatt tcggtcgacc agttaaccaa attgtctatg aacatttagc
    38881 aggtcgtatc cctttaattg caagtggcgg gattaattct ccagagtctg ctttagatgc
    38941 tttacaacat gcagatatgg tcggcatgtc ttcgcctttt gtcacagaac ctgactttgt
    39001 gcataaactt gctgaacagc gaccacatga tatcaatctt gaattttcta tggcagattt
    39061 agaagattta gccattccac atgctgcttt taaagatatt gttaaaatga tggattatgg
    39121 ggaaggtcta aagaagcata cccgtgacgc tttacgtcaa ttggaacaga attatcatga
    39181 ctcatcgtct taagactatt tttcatggaa atttaagaca gtgttataat gacatcaata
    39241 tcaaaaataa ggagcctatt aaatcgtgac tgaaattaaa gataaagtaa ttactaaaga
    39301 tgcatttgca ttaccttaca caattattaa agcaaaaaat caaccaacaa aaggcgctat
    39361 tgtctacatc cacggtggtg gattaatgtt cggtaaagct aacgatttat ccccacagta
    39421 tatcgatatc cttacggaac attacgattt aattcaacta agttatcgtt tattaccaga
    39481 agtaagtctc gattgcatta ttgaggatgt ctacgcatca ttcgatgcga ttcaatcaca
    39541 atattctaat tgccctattt ttacctttgg cagatcatca ggtgcatatt taagtctatt
    39601 aattgcacga gacagagata ttgatggtgt tattgatttt tatggttata gtcgcatcaa
    39661 cactgaacct tttaaaacaa ctaatagtta ttacgcaaaa atagctcaaa gcgtaaacga
    39721 aacaatgatt gcacaactca cttcaccaac accggttgtt caagatcaaa tcgcacaacg
    39781 ttttttaatc tacgtttatg cacgtggcac aggtaaatgg ataaatatga ttaacattgc
    39841 tgattatact gattcaaaat ataatattgc accagatgaa cttaaaacac ttccacctgt
    39901 tttcattgcg cattgtaatg gcgattatga tgttcccgtt gaggaaagcg aacatatcat
    39961 gaatcatgtt ccacattcaa catttgaacg cgtgaataaa aacgagcatg attttgatcg
    40021 tagacctaat gacgaagcaa tcactattta tcgtaaagtt gttgatttct taaacgctat
    40081 aacaatgatg taatatatca ttattttaaa aaactcaatt tatcatcatg ataaacagtt
    40141 attcgatcaa ccatattatt catacaattt attaagctca taaacagata cgcctgtccc
    40201 tttagttttc gctaatgaga caggcgcttt atttcacttt actattttta aattcaacac
    40261 tgtgatgcta cttatatctg atgtgccatg ataacagcac gatttacata gcatattaaa
    40321 ttcagaagat gaatttaact tgctttcaat tgtgttcctt tactttcagt cgcttcaaca
    40381 cgcaacaagt catttataat acctgtatag aagttggcaa gttctgcacc tttacgtttg
    40441 aaatccagga agtccacacc gataaaatcg acatgatccc atccttgtat gattggttta
    40501 acttgccaga tacctctgcg tgtggcaggt tcatcattcg taacattaac aaatggttga
    40561 ttggacggat gtaatgacga aatcactggt acgacaccat catttttacg ccattcttct
    40621 cttgcatcat gaccaataat tctactcgtt gtatccatta agaaaaatgt acctaaatca
    40681 ggattttcat aacctaatgg accagtatga gatgatacac ctgtataagt cgtatacgta
    40741 atattaggat tcatacttgt catgttgttc aattttgcag agccatctaa cgttaaatca
    40801 taggcagcat tgtcgtctga tgtccaaatt ttgcttttac taacgcgttt tatatagtca
    40861 atgtaactct catttggtaa ttgtttaaag ccccattgcg ttaatcctaa atcgatattc
    40921 gaatacttgt tacccataaa tcgatttaaa gcgaacatga tttttctaac agcttctgta
    40981 tttccaaact tatcagctgc ttgtgaacca ttatgtggtg ttgctaatgt tgtgattgat
    41041 gcaaccatat tgttatgacc accagtgaat aatggtgata tttctccacc atgcgcttta
    41101 tgataggcaa tttcttcttt gttaccattt cttaaaaact cttccattaa acgaattgtt
    41161 tgaccaccca tactatgccc tacaagatgt acctttttac caggttccca attaggcatg
    41221 attcctttat aagtcttacc atagcgctca tgtccgtatt tagctgcatg tgctgcgcca
    41281 taatctacgc gaccaccttt aatgtaataa taaagttcta cagcgcgatc atagttacta
    41341 ccaaatgcac ttacacttgc ttgatgtaca ttatagcctt gctttctcaa ttcttcgata
    41401 actttaaatt tatttccacc ccaataattt ggatataaag caggtgcatt atcgcctact
    41461 aatcctaaaa atccatgtac aaaaacaact ggatatttgt taagtggttg tacttgattc
    41521 gctttaagtg gtcgaacctt tttagcatct gctgctgcat ttgtagtcga acgtgtttta
    41581 ggcgcttgaa gcgcatcttt gattagttgt ttattatcag tatttttatc tgtcggtttc
    41641 tctggggaat tttgaccatc gatatgttta cttaaatcgc caacttgtgg tttctgttca
    41701 ctttggcgaa cagtatcatc ttgctttgca tctatgattt gatgcgcggg ttgatgtgta
    41761 tcttgttgat ttggatgttt gtccgtggtt gcatcttgtg cttttgttga tttaggtgca
    41821 gttttatcat tagacggggg tgtagttgaa gttggtgctg ctttatcatg attgggtaat
    41881 accttttgtg ccggaatgga tggttgtgat ttatcaacat cattcgattg tgacgttgtc
    41941 gattgtgatc cattacgttc agcatgatgg gtattttgaa cctgatcatt ttgacgttca
    42001 gtattattag tcgcattatt ttggtgtgac tgtttagaac cttgtcgttc agaatcttgc
    42061 tttacttgat tatcattttg cgttctgatt gttttactat tttgtgttga ttgatacttc
    42121 gataattgaa gcgtatcttt accttctgtc actgttccac ttttaccatt ctctttatgc
    42181 atatcgtcta attgctttcc tgactgcatt tgatgtgacg ttatcgcatt cccttgttct
    42241 cccggttgat ttagtgtttc tttttgtacc gctgcattag ttggtgtttt ttccgaggct
    42301 tgtgcttcat gtgatgacac aacaaacatt gtagccgcta acactgacac cacgcctatt
    42361 gaatactttc taatactata ctttctttct tcttgtcctc ttaacatata atcacctcaa
    42421 aagtattttt ttgaccccct cttgagaatg aaaaacaact gcaccttcaa tataacatga
    42481 catatattat tttttgatta agactacaaa attaataaaa atttaataat acgatattaa
    42541 atcgatacta atcatttaca aatatgtgca tttccaaata atttataaac acttttagtt
    42601 gtggcgttga atcatcacaa caacatttaa gataaatatt ttattaagta atatagtttc
    42661 aatctatata taattagtta atttgtattg gtctgaaaat aattcaaact atacaattat
    42721 aataagtttt cagatttttc taaattaaaa taatgattca tctcattaag gggaagatgc
    42781 atcatacagt tttgtgtatt catttaaaca aaggggttgc tttaatgaat caatatcatt
    42841 ctaatgcaca acaaccaagt gcatggcgtt tttttgtcta tagtttagtg ggcatactat
    42901 gtttctttat tccttttacg attaatggta acaacactat tttcgtcgat catgttcatc
    42961 tagccattcg ctcaatcata ggtccactta tgccctatgt tgcactgatt atgattttaa
    43021 ttggtacagc gttaccaata gtgagacgta cttttatgac ttcaatcaca aacttggtca
    43081 ttacattatt taaagttgca ggtgcaatga ttggtataat gtatgtattt aaaatcggtc
    43141 catcaatact atttaaagct aactatggtc cgttcttgtt tgaaaaatta atgatgccat
    43201 taagtatctt aattccagta ggtgcaattg cgctttcttt attagtaggc tatggcttat
    43261 tagaatttgt cggtgtttat atggagccta ttatgagacc tatttttaaa acaccaggaa
    43321 aatccgctgt cgatgcagta gcttcgtttg tcggcagtta ttccttagga ttattgatta
    43381 ctaatcgtgt ctataagcaa gggatgtaca acaaacgaga agccacgatt attgcgactg
    43441 gctttccaac agtttcagca acttttatga ttatcgttgc taaaacttta ggattaatgc
    43501 cgcattggaa tttatacttt tggataactt tagtcatcac atttgtcgtg actgcaatta
    43561 ctgcatggct accgccaatc accaatgaat caacagaata ttataacgga caagaaggag
    43621 aacaagaagt tgctattgaa ggaagcagac tgaaaactgc atatgcagag gcgatgaaac
    43681 aaaatgcatt aacaccatcc ctcgtgaaga acgtttggga caatttgaaa gacggtttag
    43741 aaatgactgt tggtattttg ccttctatat tatcgattgg ttttttagga ctgattgtag
    43801 cgaactatac accattcatt gattggcttg gctatatctt ctatccattt atttatattt
    43861 tcccaattgc tgatcaggct ttacttgcaa aagcgtcagc gatttctatt gtagagatgt
    43921 ttctaccatc tttgttagta actaaagctg caatgagtac taaatttgtc gtcggtgtag
    43981 taagcgtatc agccattatc tttttctcag cattagtgcc atgtatacta gcaactgaaa
    44041 ttaaaatacc tgtctggaaa ctcatcatca tttggttttt acgcgtggca ttgtcgctat
    44101 taatcaccat ccccgtcgct ttacttattt ttggataatt caaattgaaa ataaaatcac
    44161 tatcgtaaac aaacaaaacg agatgaaccc attacattca ttcagccacc taagataatt
    44221 aggtaccttt aattaatgat ggtcatctcg tttttttatc gttatcattt aatcttccat
    44281 aacttgaaca aaacgtttcg taatttcttt tggtcgtgtt atcgcaccac caacgactga
    44341 acaatgaacg cctaagtcca tcacgcgttt atacatatcc ggtgtaatga cattaccttc
    44401 cgcaataact tttgcatcaa cactttgtag tacatctttt agaaattgga agtcattttg
    44461 ataaagtaat tgtccttgcg tataactagt atagccatgt aacgtcgtgc caatataatc
    44521 aaagccaagt cgtgcggcat ttttagcttc ttcaacggtc gcgatatcag ccatgatttc
    44581 aacgttcggt gcatgtgttc taatatatga tactaattcg tctaacgttt cttttggacg
    44641 ttgctgtaac gttgcatcca atgcaatgac ttcacattgg ctttctatca gttcatcaac
    44701 ttcttttgac gttgctgtaa tgaaaacatc tgagtgatta tagtcacgtt tcacaatgcc
    44761 aataactggt aaatctaccg tttctttaat tgctaaaatg tcttccttag tatttgcgcg
    44821 aataccaaca gcaccacctt catacgcagc taatgccatt ttcgacataa taaaagatga
    44881 atgcaatggt tcatctggta gtgcctgaca agatactatt aatccatgtg gtaacattat
    44941 tcctcactcc ataagtcgtt tttttcacgt aaagtcattt acaatatatc attaagataa
    45001 tttttatcaa tgctttttac tatcttgaaa atatttttca tatataatag taatcattaa
    45061 gatgaagggg tgacacaatg aaatttgaga atcgtgttca acgttatcaa catttattta
    45121 caaaaactga taaacgcatc gtcaattata taagacaaaa tggttatagc gatgcctttt
    45181 caacgattaa ctccttaacc catgcgattg gtacatcacc agcaacgatg acacgcttta
    45241 gtcataagct ggattatgag aatttccaag atttaaaatt taatattcaa caagaaatga
    45301 cagagacagt tattgaaaat agcccaatta ttcaaagaat tcataaatat catcaacaaa
    45361 tcattcaaca aactggtgaa tttattgata acgacattat ccaaaccttt atcgacaaac
    45421 ttcaatcaag tcgacatata ctctttgctg gactaggtag ctctggttta tcagcgactg
    45481 aattttatta tcgtatgatt cgtatggggc taaaaggtaa tgttactacc gattcacatt
    45541 taatgaaaat atcggcatcc ctactatctc attcggatat gtttattgct atgtcaaata
    45601 gtggtaatac ttcggaatta atttctgcag cggaagtcgc caaatctcat ggtgcatatg
    45661 tcgttgccat cacaaatttc gaaggtagca aacttacaga ttgtgcagat ttagtacttt
    45721 taacaacgga tcaatcgcgt aataccgacc atcaatttat caacacacaa attgcgacac
    45781 tctttttaat cgatatcgtg agctatcatt tattagaaaa tacgaatctg agtcaaactt
    45841 atcaacatac taaatctatt atcctagaca acaaataact agtagtatat tcaaatccac
    45901 ctgcaactgt agacaatcat cgtgattgtc ccaacgagtt gctaggtgct tttttaattt
    45961 caataccaaa tatttttttc attttctaaa attttaatat ctcagcttct tcaatttaat
    46021 atgtctgtgg cacactgttg gttgatatag tttagattgg tcctagatag agtgtgatgt
    46081 gtggaactaa caacagctct catctctcgt aacggtttca aattctattt tagaatgact
    46141 ccttctttca actatcattg caaacagcca tataatgctg catcattttt actcttagtc
    46201 gtttgtattg gtgcataaac atagtctttt ggtaaatagt gtgcaacttt cggctcgata
    46261 tatttaatga gattatctcc ttgttcagat ataccgcccc caattaatat aagccctgga
    46321 tcatacatga cctgtatttg ggcaatccct tctgctacat cttctgccca ctcattcaat
    46381 atttgttttg caatatcatc accttcttca gctgcttcaa acaatactgg cacatgtgtg
    46441 cttctcgtaa atcctccggc aatcatgcgc tttttcaatg cactcgttgc agcacgttgc
    46501 tcaaacgttg tattttcagt tggacgatac aataaatacc caacttcatt tgccttatga
    46561 agctcaccat tatcaatatg accttgatta ttcttgtacg cacccccaat gcctgtacca
    46621 agcgtcatac aaaagatccg ttctgcttga tattgatgta atttcaattc gcctagtaat
    46681 gcagcgttta catcattttt tactttgaca taaggagaca gtgattttaa taatcgctta
    46741 aaattagtac ctttataatt cggaatggtt ggccctgcgt atacaatttc gcctttttgt
    46801 tcatcaacaa cgcctgctga tgaaatacct atgacaggtt ggatcaactg atattgcttc
    46861 atatatcctg ttacaatctc atatactttg tcagtaataa gctcactttt gttgtccggc
    46921 gttgatattt gttgatagtc aaacatattc aattgcttat caataactgc cgatttaatt
    46981 tgagtgcctc caatatcgat tgcgatgtaa tacaccttac tcactccatt tctattgatt
    47041 ttgaaaaaca ttttcattaa ttatacataa atatgactac aattttcaaa aagatattgt
    47101 tacattttat gatagcgttt acaattatag ctgtaaacta tattatcaaa tgatttttgg
    47161 agggacttca taatgaacaa agatttaaaa ggtttatatg cagcgttact cgttcctttt
    47221 gatgaaaatg gtcaagtaaa tgaacaagga ctcaaacaaa ttgctcaaaa tgccattgaa
    47281 actgaagaat tagacggtct ttatgtaaat ggtagctcag gcgaaaactt tttattaaat
    47341 acagagcaga agaagcaagt tttcaaagtt gccaaagagg cagttgggga taaagtgaaa
    47401 ttgattgctc aagtaggttc gttagattta aatgaagcca ttgaacttgg aaaatatgcc
    47461 acagaactcg gttatgatgc actttctgcc gtaacaccat tctactatcc atttactttt
    47521 gaagaaatta gagattacta tttcgatatt atcgaagcaa ctcagaacaa tatgattatt
    47581 tatgcaatac cagatttaac aggtgtgaat atttctattg aacaattcag cgagctattt
    47641 aatcatgaaa aaattgttgg tgttaaatat acagcgccaa acttcttcct actcgaacgt
    47701 attagaaaag cattcccaga caaattaatc ttatctggct ttgatgaaat gttagttcaa
    47761 gctacgattt ctggcgtaga tggtgcaatt ggttctacat ataacgttaa tggtcgccgc
    47821 gctagaaaaa tcttcgactt agcaagacaa ggtcaaattc aagaagctta ccaactacaa
    47881 catgattcaa acgacattat cgaaacagtg ttatcaatgg ggatttatcc aacattgaaa
    47941 gaaatattac gtcatcgtgg cattgatgca ggattaccga aaagaccttt caaacctttt
    48001 aacgaagcac atcgacaaac attagatcaa ctcattgcaa aatacgattt atagacatca
    48061 aagaatcaat acattgtaaa ggggatgttg cccatgaaag aaattggatt tggcacacta
    48121 aactgggttg ccgttatcat ttatctacta gctatgttgt tcattggcgt ttattttacc
    48181 aagcgcgcga gccaaagtac caatagtttc tttaccgcaa gtggtcgctt gccatcttgg
    48241 gtagttggct tttcaattta tgctactacg ttaagtgcga ttacatttat gtcgacacca
    48301 gagaaagcat ttttaacaga ttggtcatat atcgctggta acattgctat cgtcgcaatt
    48361 attccattac tcatttattt ctatgtccct ttctttaaaa agttaaaagt aacatcagca
    48421 tatgaatatt tagaagctag atttggccct agcatacgtg ttattggctc attattattt
    48481 gtcgtttacc atttagggcg tgttgcaatc gttatctact taccaacatt agcaatcaca
    48541 tctgtatcag acatgaaccc ttatatcgtt gcatcactcg ttggtttact atgtatttta
    48601 tatacatttt taggtggatt cgaaggtgtg gtttggagtg atttcattca aggcgtcatt
    48661 ttattaggcg gcgctttagt tatcattatt ctaggtgtta tgaacattaa aggcggtttt
    48721 ggcactgtct ttgcagatgc gattgagcac aaaaaattaa ttagtgcaga caattggaaa
    48781 ctaaatactg cggcagctgc cattccaatt attttcctag gaaatatttt caacaacttg
    48841 tatcaataca cagcgagtca agacgtcgtg cagcgttatc aagcttctga tagtttaaaa
    48901 gaaacaaata aatcgttatg gacaaatggt atcctagctt taatttcagc ccccttattt
    48961 tatggtatgg gtacaatgct gtattcattt tatacacatg aagctgtttt accaaaaggc
    49021 ttcaatacat catctgtagt gccatatttc attttgactg agatgccacc atttgtagca
    49081 ggattactta ttgcagccat tttcgccgct gcacagtcta ccatttcatc tagtttaaat
    49141 tctatatctg cttgtatttc aatcgacatt aagcaacgct tcttcggaaa aggtagcgag
    49201 cgacacgaag ttaactttgc acgtttcatt atcatcattg caggtatttt cggttttgga
    49261 atgtcactat acttaattgc ttctaattca aatgacttat gggatttatt cttgtttgtg
    49321 actggattat tcggcgttcc attggctggt gtatttgcag ttggtatttt cactaaacgt
    49381 acgaatacat tcggtgttat ttgtggatta atattgggta tcatctttgc ttatgtctat
    49441 aatggtgttg gcaaaggtaa ctcacctttc tatgtatcta ccatttcatt tacagttgct
    49501 tttgtctttg cttatatact tagcttcatt gtcccttcaa aacataaaaa agatataacg
    49561 ggattaacaa tttttgaaaa agataaacca tcaacataca tttcaaaaat ggctacgaaa
    49621 aagtagattg ttatgataaa accccgtcac taagttatga tgcgctgttg cgccaacttg
    49681 gtgacggggt tttgatgttg ttatgaattt aatttaggta cttcgattaa attataatac
    49741 taggccaatg attgatcctg aaatgataga agctagagtt gaaccaagta gcaacctcat
    49801 tgcaaaggat gcaacttttt ctccttgttt atcactaatg cctttaattg aacctacgat
    49861 gataccaacc gtaccaaaat tagcgaagct tactaagtaa actgaaatga taccttgtgt
    49921 tcgagctgat acatcaccca ggacattttt aaaatcaagc attgctacaa actcatttgt
    49981 aattaattta gtcgccatta aagagccagc tggaacagct tcgctccatg gaatccccat
    50041 taagaatgcg attggtgcaa acacatagcc aataagctgt ttaaagttca aaccaacact
    50101 accaaacatg atattaattg cttccattaa tgaaataaat gctaacaaca ttacggctac
    50161 tacaacagcg attttaaacc catccatcgc actatcacca atcatttgga aaaaggcaac
    50221 tttcttaggt tttcctgttt ttccattcac tgatttcgtt tccgttgatt tagttaagtt
    50281 atcaatttca acatcactat catcagattt atagggattg attacactgg cgatgataag
    50341 cgcactaaaa atatttaaca ttacagctgt aactacgaac ttgggctcaa tcatctgcat
    50401 atatgaaccc aacattgcca tactaacagc actcatacca gacgtcgcaa ttgtatataa
    50461 tttcgctcta gataatctcg gaataatatc ttttattgtt aaatatactt ctggttgtcc
    50521 aaacattgct gttgaaatag caaagtaact ttctaagcgc cccattctcg ttattttatt
    50581 aatagcgata cctacatatt tgataataaa tggtaatacc ttaatataat taaaaatgcc
    50641 tattaataca gaaatgaaca ctaatggcag caacacattt aaaaagaacg taaagccatt
    50701 tttattttgt atatctccaa aaacaaagtt tatacctgct ttactaacat ttattaaccc
    50761 ttcaaaaaat gagcctaatg cagttaagat tgttaaacca atcgttgtgt tcatcataaa
    50821 taacacaatc aacacttgaa taaatatcat tattaacgtc tttttgaagt ctatgttttt
    50881 acgatcaaaa ctgaataaaa acgcaacaaa tagtgcaaat gctatccctg tgatagcgaa
    50941 taaaatattc atgtatcatc acctacaata atttattaac agcgacagca attttagcac
    51001 ccaacgccgc attgttttca acaagtttta tatttgctgc taaactttta ccattcgttt
    51061 tttctacaat tttccctaac aagaacggtg tggcgtcctt acctttaata ccttgatttt
    51121 ccgcttcaac aacagcttca tttatgattg cctcaatata tgcttttgat aaggcatgct
    51181 catatggaat tggattagca acaacaatgc caccttcaag atttaactgc tgttttgtta
    51241 aatgaatgtc agcaagtcgt tctggcgttt caaccgaact tgttaactta acaccgcttt
    51301 cgcgagtgaa gaatgctggc aattcattcg tttgatatcc aataactgga acgccttttg
    51361 tttctaaata ctccatcgtc ttaggtaagt ctaaaattga tttggcacct gcacagataa
    51421 cagtgacatt tgttttagac agttcttcta agtctgctga aatgtccatc gtatgttctg
    51481 cacctttatg gacgccccca atacctcctg taacaaaaaa ttgaatacca gccattgcag
    51541 cacatatcat cgtcgtcgct acagtagtag caccaacaca cttcatcgca acaacttctg
    51601 ctaaatccct tctagatact ttagcaacgt ctttactagt tgccagtatt tctaaatctt
    51661 cgctttctaa accaatttta attttgccat ctataatggc tatggttgct ggaatggcac
    51721 cattattcct gataatttgc tctactgttg ttgccatttc aacattttgt gggtacggca
    51781 taccatgcga tataattgtt gattctaatg ctacaatcgg ttgattgttc tccagtgctt
    51841 gctgaacttc tcgagaatac tcaatatact tttgtaaatt tgccattttt ataatcctcc
    51901 atatcgtgat aaagttgctg ttgatctagg ttttgcctaa ctgtatattt cgtttctatc
    51961 gttttctttg cgttaaccat accagcaatt aatatatctt cagtagacat cccatttaac
    52021 cagctataca ctactgcagc acagaatgaa tcgcctgcac ctgtaacatc tttcacacta
    52081 tttgatggca taactgactt aatgatttct tcctcaccac ttcgataaat gagttctttc
    52141 acgccatttg tcacaataac atttttaaca cctaaatcat tccagcgttt agcagctatt
    52201 tttaaatcat cagtagattc tatttttaaa tttaagtatg tttctgtttc atctttattc
    52261 gtgataatcc aatcaatagc atgtaatgaa tcaggcatat ttttcatttt tggagaagaa
    52321 accgtggtga taactaattt gatttgatgt ttcgtggtat aggcacataa gaagtttaat
    52381 gcctctttgc ctaaattcaa atcgacaata atgcacttag cctttttcaa taagtgtgaa
    52441 cgcttaatta aaaattcagg cgtaatgtag tcaaacactt ccatatctgc taagccatat
    52501 gtcatgtcgc cttctttact aattaaagct gtatatgaac ctgtactcgc attttcaaat
    52561 tgttgaacat gatccaaatt cataaatggt gtggacaatc gtttaatcat ttcccattca
    52621 ctatcttgtc cactagcaga taaaaaagcg accgtttcgc caagcctacc taagttctca
    52681 gcaatatttc ttgctacgcc accaatagag cgtgttgacg ttacaggatt tgatgtttct
    52741 gcaactaaat ttttatgcac ataaaactta cgatctacat tcgctgcgcc aatacaaaca
    52801 ataggataat cttcatttaa aacatatgcc tttcccataa catattcctt ttgtattaat
    52861 cctgaaataa tgtttgctac gctgggtcta gataatccaa ttgcctcagc aagttcacgt
    52921 tgtgaaataa acggattatc tttaattctt tttaaaattt ctttctcaga atcgctcata
    52981 acatccctcc tcatattgac ttaaaatact tgaatcagtg aattcacaaa aatatcgtag
    53041 catgctgtta tttctgttgt aaacgcttac atttaccgtt atatcaatga tatttgttta
    53101 taaaatacgc ataatttcgt aaaaagtaag ttcattccat cgtgtaaaag gtacttgaga
    53161 aaatgataaa tgtttataca gtatgatatt tgtcgacaat aaattatgac atttttatgt
    53221 cttatatttt tctataaaaa aacgtgactc attatcgact gttacatcga ctgaatcacg
    53281 ttactatgtt ttatttgttt ttactattca atatgatgat gctatgcgtc tactgcacct
    53341 cgattaatca tgtcttgctt agtaacaatt ggatattttt taaatacaat tgaaccaaga
    53401 taacctgcaa gcgcacctac aatcgccatt actacaccat aaatcacaat tgtcgtcgga
    53461 tgattaaatc caaacataac taaaaatcct gcaatcggtg tagctgtacc tgtcgcatcg
    53521 ttaattaaac ctgacatagc aataattaaa ccagcaatcg caccaccaaa gaaatttgta
    53581 atatagattg gaattggatt ggctgataca atatctgctt gtgataaagg ttcaatactt
    53641 actgcaatcg tagacttacg atcacctaat tttaagcgat ggaatagcgt cccattcata
    53701 aatgccgaac taaatgctgc catggcacca atagccatag gtacacccgt taaacctaat
    53761 aatgctgtca atgccattga actcaatggc gctgtaccga caaccgtgat aataccacct
    53821 aaaatgatac ccatgataat tggattcgta ttcgtactac tttggataat atcacctatt
    53881 cgaatcaatg tactattcac tactggcgtt aataatactg caataagacg agctaatggc
    53941 gctaagatga caatcgatcc aattaagtcg acaccatccg gcacatattt ctctgtatat
    54001 ttcatcacat atccaatcat ataacccgca aagaaacctg gtaataaatc cataccaccg
    54061 cacgcggctg ctataactaa tgcatatact ggtgacacac cgatagctaa tgctgttaat
    54121 ccagcggcag cgacaccacc tagacctccc gcagcgtctc ctagctcttc taaaaattta
    54181 ataccgaata cttcgccacc aacatattta ttaaatgctt ctactaaaaa tgttgcgatt
    54241 gctgcattag ctaatgctcc catggcacgc ataccattag gtgctttata tgtaaataat
    54301 gtaaaaatca ctaagaccaa aaataaaaat aaagtaccta tcaataaatc cataaccatt
    54361 gacccccatt acgataattt tatattcaat ccccatattt aaatattgaa tacttagagg
    54421 ccattttact acaaaaaata ttatatttca cggaatattt ctaattttcg ataataatta
    54481 ttattaattg aatgtgaaag tttattcaaa taatacataa acataaaaat accggcacat
    54541 gatgtaccgg cttaataaaa ctattctact gtttcatttt caagattaag gacgccatct
    54601 ttcatatgat atgaacgatc acaatatgct ttaagtcgtt catcatgtgt aacaataata
    54661 catgctttct ttctttgttt ggcttgatca cgtagaattt taatgacttc aatcgcattt
    54721 tcagtatcta acgccgcggt aggttcatcc gctaaaataa tcgacggatt cgtatataac
    54781 gctttagcta tcgccacacg ttgtttctga ccacctgaaa tttctgaagg taacttatta
    54841 agcaatgaag ttagccctaa ttgtgacata agttgctgat agtcttcatt agacataaca
    54901 ttcttatttt tctttttcaa taatgtaaat tgttgcttta ccgttaaaaa tggcacaagg
    54961 tttgtagctt gtaaaataaa acctatttca gacattctaa cttttgccaa tgctttttgc
    55021 ttcattgtcg taatatcttg gttattgatt aaaatgtgcc cagatgtcgg cgtttgtaaa
    55081 gcacctgcca tagttagaaa tgtactttta ccagagccag aaggtccaac caatgctata
    55141 atatcacctt tatttatctc aaaatttgta tctttaaccg cttcaatgtt acgattccca
    55201 tctttaaatg actttgttac attttcaaat ttcaacattt gctacacctc ctacccaatc
    55261 gcctttaacg gatctatttt tctaattgtt aaaatggaga ataaacttcc taatacagag
    55321 acaatcatta acacaattgc aaatacgagt aatgttagta catcgaattt gacaggtact
    55381 gcatcaggta aaaatgcacc tgtaacgcct gttaacagta agccaaatgc cgtaccaaat
    55441 aatgctaata tgagcgtctg cgaaataact acgttcgcta aatagccatt cgtaaatcct
    55501 tgagccttta atatgccaaa taaacttgtt ttttgtaatg tcatgacata taagaaaatg
    55561 cctataactg tagctgaaat gacaaataag aatgaaatca taaagtttaa tgttaagttc
    55621 tgtggtttat aacctggtaa attttcaata aagtcattaa tacttaccgc ttcaagctct
    55681 tggtttaatt ttttgtcttt ccaatttgta tcacgtacaa caactgcatt aattttatct
    55741 ccagtcaatc taggattgat cttggcaatg gtagcgtcat tcgtgaaaat gactggtgac
    55801 gcattatatt ttgcactttc tgtaaaacct acgatatgca atttttcatc tgattgagat
    55861 agtgataatg tgtcgccaat tttaaatcct ttatttttaa gtgtttcatc agctaacact
    55921 tcattatctt tagtcgcttt atgcccttct attaaactcg gaactaaaaa tgatgacttt
    55981 tcaacaccga acactaaaac attgtctttt tgatggccat tagacacaat ttcccctgtt
    56041 tgcttcaaag tagcttgctt cttgtattta ttttcaatat ctttcttgtt aaaaacagat
    56101 tgttgcacag tttgattggc atctttattt agaacaatgg cctctgcttg ccacttatca
    56161 ataccttctt tattcatatt gataagacca ttcgccaatc cagataataa aaatagcaag
    56221 taactaatca tcgttaacac accaataatt agtccaaact tcaatttgtt gcgccgtatt
    56281 tcattccaag ctaaaaacat gcatttctct ccctactact atgatttaaa cattgtttat
    56341 attcttagat gcacgtacgt cgtgttgcgc tctgtaatgt tatacataca cttatccttc
    56401 attatacccg aactttttat attaaaagca aatttatgga aaatgcaatt aattgtctat
    56461 tatttttgta cggtacattt aaaatttaag gatcaattta aaaacgccta catatacctt
    56521 taagtacatg tagacgtcca attcatatat tatttaactt cgcctgtttt aggatcgaat
    56581 tgcttaatag catttttacg taatttatct tttgctttgt cacttgcttt atagttattg
    56641 ttgtaaattg tagcttccca actaccatac attgggttag ggaaaatgat atatttctta
    56701 ccgaaatcgt ctttatgttt ttcaattaat gcttcacgag attcagctgt agcttctttt
    56761 ggatctgtaa agtctaataa attatctcca aatagcatga caagtttatg atccttttga
    56821 accatttgtc tgcgtgattc tttactctta tcatctttac cttttagtaa aatatgactc
    56881 ttcttagctt gagggatacc ttgttgtttt aagttctttt gtgttgcctt taaatctttt
    56941 tctttatctc tatcagaaat atagtagata tcgacatctt ttttgtcagc atatttcaag
    57001 aattcttttg cgccatagac aggtttagct ttagcagctt gtacccattc atgccaacct
    57061 tctgggaaag gtttattatg tattgatgca tagccttgat atggagaatt atctaaaact
    57121 gtttcatcta aatccaaagc aatagctaac ttatgtttac ctttattctt tttaatctct
    57181 ttatctagtt gaactttcgc actgttataa ccttgtaaat ataatgcttt tgcttcagct
    57241 gaattttgat accaagccac tgccataata ttttgattac caagattcac cttttgtgat
    57301 gctggtatag aagcttgttg cgtttgttga acttcagcag aacttttggc aaacgctgta
    57361 gaatttgtcg tttgtggtgc tgaaactgta accgctaccg ataatgatgc tattgcaata
    57421 tactttgaaa ttttattcat cttatcacct catgattaat atttaaaata cagttaaaat
    57481 tataaatgca tttatttaat attgctatac tatgaaaaga tatttaatat tatttcttga
    57541 aaagctaaca aatatgtgaa catttcatat aagcatgatt taatgggaga cctatcacat
    57601 gagagagatt tgtgatttat cacgtaataa acttaaattc atttgagtga ctaggccttc
    57661 ctatcagaca tattcactca tccacgtatc attatgtgta cagtgtgcta tctcttattt
    57721 acctattgga acaactataa actcatccat agtttacctt ttataaatag cagtcctcac
    57781 tcatacaatt tctcataaaa atcacaacgc tccaacgtat ttccaactta ttttcactta
    57841 ttttaattca taaaaacgac actttaattg tcattatcca ataatagcaa gacgttatta
    57901 ttgcaatctt ttttataaaa taatagaatc atagtattgt catttaaaga taaagtaaga
    57961 acgtttttat ttttcagatt ttttaaatta ttatgaatat ctagttttag gaaggaaatt
    58021 acattgaaaa aacaagttat tatttcgggc ctcatgttat tttcactatt ttttggagcc
    58081 ggaaatttaa tattcccgcc catgcttggc catacagcgg gtcaaaatat gtggattggt
    58141 atgctaggct ttgctcttac aggcatatta ctccccttta ttactgttat tgttgttgca
    58201 ttttatgatg aaggtgttga aagtgtaggc aatcgtatac atccatggtt cgggtttatt
    58261 tttgctgtcg tgatttacat gtctatcgga gcattttacg gtattccacg tgctgcaaat
    58321 gtcgcgtacg aaattggtac aagacacatt ttacctgtgc acaaccaatg gactttaatt
    58381 atattcgcag caatcttttt tgccatcgtt tactggatta gtttaaatcc atcgaaaatc
    58441 gttgataatt taggtaaatt attaacaccg ttattactat taatggtctc tctattaagt
    58501 attgctgtca ttttcaaccc tgaatctgca ctaagtgcac ctaaggataa atatataaca
    58561 catcctttca tttcaggaag tttggaaggc tattttacaa tggatcttgt tgctgcgtta
    58621 gctttttccg ttgtcattgt caatggctat aagtttaaag gcctcacaga tcgcatgaaa
    58681 attttaaaat atgtctgctt ttcaggtctt attgcagcca tattacttgg aatgatttac
    58741 tttgcacttg catacgttgg ggcatcaaca gctccaggaa actttaaaga tggtacagat
    58801 atattgacgt acaactcatt acgagtattt ggttcgttcg gtaacctcgt atttggaatg
    58861 acggttatcc ttgcatgcct aacaacatgt ataggactcg tcaatgcttg cgccacattt
    58921 actaagaaac acgtacctaa gttttcttat aaaatattcg cacttatttt ctctatcata
    58981 gggttcttat ttacaacact tggtttagaa atgattttaa aaattgctgt cccattattg
    59041 acattaatat atcccgtgtc tattgcactt gtactgatat catttgctaa catgtttagc
    59101 acattcagat tcagttgggc ctatcgactc gcaactgtta ttacattgat tatttcaatt
    59161 ttacaaatac taaatagttt taacttatta cacggtgtta ttttgaaatc gtttatgatg
    59221 ttacctttag cagatatcga tttagcttgg cttgtaccat tcatgctctt tgctattatc
    59281 ggtttcataa tcgatgtatt tatacgccgt ccgaaacaag ctacaactta atagacgcat
    59341 aatctcctag taatgatcgt cgcatcatta ctaggttttt ttgtatgaaa atctgtattt
    59401 attcattgat actaactgac gattaattat tttaaaataa atgatttgtt aaaaaatcct
    59461 agctgttatt caaaaatatt agtttttaaa attaattttg tgaaattatt cacaaataag
    59521 acgattcata gtatactctg attaacaaat gaaaagtgag gatgtcattg tgaaggagaa
    59581 acatattaaa tgggataaaa tattttatgg ggacgactgg gtaaataacg tagtcgaaac
    59641 aataagaaca aaagatatat tacaaagcgt ttatttaaaa cgttatttat tacgcgcgat
    59701 gatggcagga tttattatcg ggattattac ggtcttcgta ttatcagtta aagcaacaca
    59761 cgaaccagat ttaccgccag gcattgtgaa tatggccagt gccattacat tcagctttgc
    59821 gttagtactc attttattta caaactccga actactaacc agtaacttca tgtactttac
    59881 tgtaggcctg tattataaag taattaaacc gactagagta ttgaaaatat ttttattgtg
    59941 ctttgcagga aatattttag gtgctgccat tttatttagt ttcatgcgtt tttcaaatgt
    60001 aatgacgcca gatatgttaa atcagttatc agcagttatt gagcacaaaa cgttgtctac
    60061 tggttttgta agtatattaa tgaaagcgat attcgccaat ttctttatca atatttcatt
    60121 agttattgcc atgcaaattg acgatgtact cgcaaaaatg tttgtcatga tgttcggtgt
    60181 taccatattc gcctttatgg gttatgaaca cgtcgtttac aacagttgct tatttatggg
    60241 tggtttaatt tatcaagttg atacattgca cttcatacca gctatttcga atatcgctgc
    60301 ggcttttatc ggtaactata ttggaggagg actcattata ggtttattct atgcttactt
    60361 gaatgatcat catcaatttt ataaaaataa ttaactctat ttgaacaatg cgttagtcga
    60421 tgcctttaat atttagaagt gcattgtcta acgtattttt ttaaagttcg tcaatggttt
    60481 ttgtccgatg ctatgagcat tatcaaatac aaaatgctct cttaaaagca gttattgact
    60541 gaaaatctac ttctaagaga gcactttatt taatcggtta atttattaaa aactaacaga
    60601 aatataatta cttaagaaat cttgaaattt caatatacga tgtttatgat aagtcgctta
    60661 tttcatcttt aggcttgtta ttagtaagta gtttaatacc actgactaac cataaagcaa
    60721 atgtaattat gttaccactt attacagctc caataatcaa caatatacca ctcatttttt
    60781 tgtttttaga tgctttaaac ataccgattg cacctaaaat aattgaaatg attccaaata
    60841 tgaataggaa taagaataat acagtgaaaa ttgctgctgc tgtttctgca tcaactgggt
    60901 caacctcacc attaactgtt gttgacacat ctgtagtcat tacacctgat gctgctaaaa
    60961 ccatcaatag taaactaaat accgttgcta aaccctgaat aacaattcct attattgtca
    61021 ttattttgat cgatttttcc attgacttac tcctttatta ttatttacaa acttacgttt
    61081 gtgttcttat atataaatgt ttaaatttat atcgtgttta ttttaaaaat tattgtcgct
    61141 aaagctcatt tactttttga aacaggttaa aagttttaaa gaaagcctat gttaatcttt
    61201 ttccacttct acattttcat cttcataatc tgctttatgg tctttcatat acttaattgg
    61261 gtctatttct ctattaatct ctgccttacc atcttttatt tcatatgcga aataatacat
    61321 tttttcacga tctttatata attgaatacc aataacaatg actttaccat ctttataaac
    61381 atgtgtatta ctgtcttctt gtttaatgtc tgttttaata gtgttttgta ctttcgaggc
    61441 aattttattc atttgattct gttctttgta cactttatca atatcttttt gatacttttc
    61501 acctttatta gaacaacctg ccaatataat tgctaacatt aaaaatacta ccaatattct
    61561 tttcatcatt tttctcctta gatggctgtc attttccaca cactacaata ctgataatat
    61621 gctttatcat tgtgatgttt gaacaatgtt ttgccaattg ttatcaactt aaaattcgtt
    61681 taaatgtttg acgcaagatt ttgattaatt atgatattaa agtcttctta atataaacat
    61741 ctatttcagt gttaatttac aactatctac tgaaaattat gtcatcataa taaattatgc
    61801 aataactatt tgataccttt ata
//
LOCUS       DAMZLE010000004        52182 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMZLE010000004 DAMZLE010000000
VERSION     DAMZLE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.004"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            130..912
                     /gene="modA"
                     /locus_tag="POB92_000214"
     CDS             130..912
                     /gene="modA"
                     /locus_tag="POB92_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6577740.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            926..1597
                     /gene="modB"
                     /locus_tag="POB92_000215"
     CDS             926..1597
                     /gene="modB"
                     /locus_tag="POB92_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI6577741.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYHRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1598..2203
                     /locus_tag="POB92_000216"
     CDS             1598..2203
                     /locus_tag="POB92_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6577742.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            2373..3377
                     /locus_tag="POB92_000217"
     CDS             2373..3377
                     /locus_tag="POB92_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDI6577743.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLIDDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWDGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            3408..3914
                     /locus_tag="POB92_000218"
     CDS             3408..3914
                     /locus_tag="POB92_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDI6577744.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3916..4410)
                     /gene="moaC"
                     /locus_tag="POB92_000219"
     CDS             complement(3916..4410)
                     /gene="moaC"
                     /locus_tag="POB92_000219"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDI6577745.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNKTIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            4473..5732
                     /locus_tag="POB92_000220"
     CDS             4473..5732
                     /locus_tag="POB92_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDI6577746.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5729..6214
                     /gene="mobB"
                     /locus_tag="POB92_000221"
     CDS             5729..6214
                     /gene="mobB"
                     /locus_tag="POB92_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDI6577747.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            6228..6674
                     /locus_tag="POB92_000222"
     CDS             6228..6674
                     /locus_tag="POB92_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDI6577748.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6680..6913
                     /gene="moaD"
                     /locus_tag="POB92_000223"
     CDS             6680..6913
                     /gene="moaD"
                     /locus_tag="POB92_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDI6577749.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6920..7519
                     /gene="mobA"
                     /locus_tag="POB92_000224"
     CDS             6920..7519
                     /gene="mobA"
                     /locus_tag="POB92_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDI6577750.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            7539..8561
                     /gene="moaA"
                     /locus_tag="POB92_000225"
     CDS             7539..8561
                     /gene="moaA"
                     /locus_tag="POB92_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI6577751.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(8843..9013)
                     /locus_tag="POB92_000226"
     CDS             complement(8843..9013)
                     /locus_tag="POB92_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577752.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            9139..9489
                     /gene="sarV"
                     /locus_tag="POB92_000227"
     CDS             9139..9489
                     /gene="sarV"
                     /locus_tag="POB92_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDI6577753.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(9591..10802)
                     /locus_tag="POB92_000228"
     CDS             complement(9591..10802)
                     /locus_tag="POB92_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6577754.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYPIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(10795..11235)
                     /locus_tag="POB92_000229"
     CDS             complement(10795..11235)
                     /locus_tag="POB92_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDI6577755.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHKILLKETHND"
     gene            11397..12161
                     /locus_tag="POB92_000230"
     CDS             11397..12161
                     /locus_tag="POB92_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6577756.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNNIEAVKN
                     KLQSERVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(12153..12404)
                     /locus_tag="POB92_000231"
     CDS             complement(12153..12404)
                     /locus_tag="POB92_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577757.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLAIIHMGILAHSTYVYLY"
     gene            12793..14058
                     /gene="femX"
                     /locus_tag="POB92_000232"
                     /gene_synonym="fmhB"
     CDS             12793..14058
                     /gene="femX"
                     /locus_tag="POB92_000232"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDI6577758.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            14175..17342
                     /locus_tag="POB92_000233"
     CDS             14175..17342
                     /locus_tag="POB92_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDI6577759.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTFAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVVTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGNIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(17526..17843)
                     /gene="mspA"
                     /locus_tag="POB92_000234"
     CDS             complement(17526..17843)
                     /gene="mspA"
                     /locus_tag="POB92_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDI6577760.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTMMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(18088..18252)
                     /locus_tag="POB92_000235"
     CDS             complement(18088..18252)
                     /locus_tag="POB92_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577761.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            18385..19293
                     /locus_tag="POB92_000236"
     CDS             18385..19293
                     /locus_tag="POB92_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDI6577762.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            19464..19595
                     /locus_tag="POB92_000237"
     CDS             19464..19595
                     /locus_tag="POB92_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577763.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(19712..20575)
                     /locus_tag="POB92_000238"
     CDS             complement(19712..20575)
                     /locus_tag="POB92_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDI6577764.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(20756..21643)
                     /locus_tag="POB92_000239"
     CDS             complement(20756..21643)
                     /locus_tag="POB92_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6577765.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            21709..21804
                     /locus_tag="POB92_000240"
     CDS             21709..21804
                     /locus_tag="POB92_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577766.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            21830..23965
                     /locus_tag="POB92_000241"
     CDS             21830..23965
                     /locus_tag="POB92_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI6577767.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            24078..25412
                     /locus_tag="POB92_000242"
     CDS             24078..25412
                     /locus_tag="POB92_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI6577768.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(25603..25992)
                     /locus_tag="POB92_000243"
     CDS             complement(25603..25992)
                     /locus_tag="POB92_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577769.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLIFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            26349..26657
                     /gene="rpsJ"
                     /locus_tag="POB92_000244"
     CDS             26349..26657
                     /gene="rpsJ"
                     /locus_tag="POB92_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI6577770.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            26685..27347
                     /gene="rplC"
                     /locus_tag="POB92_000245"
     CDS             26685..27347
                     /gene="rplC"
                     /locus_tag="POB92_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI6577771.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            27374..27997
                     /gene="rplD"
                     /locus_tag="POB92_000246"
     CDS             27374..27997
                     /gene="rplD"
                     /locus_tag="POB92_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI6577772.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            27997..28272
                     /gene="rplW"
                     /locus_tag="POB92_000247"
     CDS             27997..28272
                     /gene="rplW"
                     /locus_tag="POB92_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI6577773.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            28305..29138
                     /gene="rplB"
                     /locus_tag="POB92_000248"
     CDS             28305..29138
                     /gene="rplB"
                     /locus_tag="POB92_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI6577774.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            29205..29483
                     /gene="rpsS"
                     /locus_tag="POB92_000249"
     CDS             29205..29483
                     /gene="rpsS"
                     /locus_tag="POB92_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI6577775.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            29512..29865
                     /gene="rplV"
                     /locus_tag="POB92_000250"
     CDS             29512..29865
                     /gene="rplV"
                     /locus_tag="POB92_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI6577776.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            29889..30542
                     /gene="rpsC"
                     /locus_tag="POB92_000251"
     CDS             29889..30542
                     /gene="rpsC"
                     /locus_tag="POB92_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI6577777.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            30545..30979
                     /gene="rplP"
                     /locus_tag="POB92_000252"
     CDS             30545..30979
                     /gene="rplP"
                     /locus_tag="POB92_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI6577778.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            30969..31178
                     /gene="rpmC"
                     /locus_tag="POB92_000253"
     CDS             30969..31178
                     /gene="rpmC"
                     /locus_tag="POB92_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI6577779.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            31202..31465
                     /gene="rpsQ"
                     /locus_tag="POB92_000254"
     CDS             31202..31465
                     /gene="rpsQ"
                     /locus_tag="POB92_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI6577780.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            31497..31865
                     /gene="rplN"
                     /locus_tag="POB92_000255"
     CDS             31497..31865
                     /gene="rplN"
                     /locus_tag="POB92_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI6577781.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            31901..32218
                     /gene="rplX"
                     /locus_tag="POB92_000256"
     CDS             31901..32218
                     /gene="rplX"
                     /locus_tag="POB92_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI6577782.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            32245..32784
                     /gene="rplE"
                     /locus_tag="POB92_000257"
     CDS             32245..32784
                     /gene="rplE"
                     /locus_tag="POB92_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI6577783.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            32807..32992
                     /locus_tag="POB92_000258"
     CDS             32807..32992
                     /locus_tag="POB92_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDI6577784.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            33024..33422
                     /gene="rpsH"
                     /locus_tag="POB92_000259"
     CDS             33024..33422
                     /gene="rpsH"
                     /locus_tag="POB92_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI6577785.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            33447..33983
                     /gene="rplF"
                     /locus_tag="POB92_000260"
     CDS             33447..33983
                     /gene="rplF"
                     /locus_tag="POB92_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI6577786.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            34014..34373
                     /gene="rplR"
                     /locus_tag="POB92_000261"
     CDS             34014..34373
                     /gene="rplR"
                     /locus_tag="POB92_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI6577787.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            34394..34894
                     /gene="rpsE"
                     /locus_tag="POB92_000262"
     CDS             34394..34894
                     /gene="rpsE"
                     /locus_tag="POB92_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI6577788.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            34911..35090
                     /gene="rpmD"
                     /locus_tag="POB92_000263"
     CDS             34911..35090
                     /gene="rpmD"
                     /locus_tag="POB92_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI6577789.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            35107..35547
                     /gene="rplO"
                     /locus_tag="POB92_000264"
     CDS             35107..35547
                     /gene="rplO"
                     /locus_tag="POB92_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI6577790.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            35547..36839
                     /gene="secY"
                     /locus_tag="POB92_000265"
     CDS             35547..36839
                     /gene="secY"
                     /locus_tag="POB92_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI6577791.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            36856..37503
                     /locus_tag="POB92_000266"
     CDS             36856..37503
                     /locus_tag="POB92_000266"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI6577792.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            37696..37914
                     /gene="infA"
                     /locus_tag="POB92_000267"
     CDS             37696..37914
                     /gene="infA"
                     /locus_tag="POB92_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI6577793.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            37946..38059
                     /gene="rpmJ"
                     /locus_tag="POB92_000268"
     CDS             37946..38059
                     /gene="rpmJ"
                     /locus_tag="POB92_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI6577794.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            38082..38447
                     /gene="rpsM"
                     /locus_tag="POB92_000269"
     CDS             38082..38447
                     /gene="rpsM"
                     /locus_tag="POB92_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI6577795.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            38471..38860
                     /gene="rpsK"
                     /locus_tag="POB92_000270"
     CDS             38471..38860
                     /gene="rpsK"
                     /locus_tag="POB92_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI6577796.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            38935..39879
                     /locus_tag="POB92_000271"
     CDS             38935..39879
                     /locus_tag="POB92_000271"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI6577797.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            39896..40264
                     /gene="rplQ"
                     /locus_tag="POB92_000272"
     CDS             39896..40264
                     /gene="rplQ"
                     /locus_tag="POB92_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI6577798.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            40802..41611
                     /locus_tag="POB92_000273"
     CDS             40802..41611
                     /locus_tag="POB92_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDI6577799.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFSIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQTITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVGEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTAIEIFDHAEELTTIGLDLPFPIKINQMLGYQTSFLTYEGLVDQL"
     gene            41608..42468
                     /locus_tag="POB92_000274"
     CDS             41608..42468
                     /locus_tag="POB92_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDI6577800.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            42458..43264
                     /locus_tag="POB92_000275"
     CDS             42458..43264
                     /locus_tag="POB92_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6577801.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            43269..44072
                     /gene="truA"
                     /locus_tag="POB92_000276"
     CDS             43269..44072
                     /gene="truA"
                     /locus_tag="POB92_000276"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI6577802.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTATAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            44312..44749
                     /gene="rplM"
                     /locus_tag="POB92_000277"
     CDS             44312..44749
                     /gene="rplM"
                     /locus_tag="POB92_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI6577803.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            44763..45161
                     /gene="rpsI"
                     /locus_tag="POB92_000278"
     CDS             44763..45161
                     /gene="rpsI"
                     /locus_tag="POB92_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI6577804.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            45508..45690
                     /locus_tag="POB92_000279"
     CDS             45508..45690
                     /locus_tag="POB92_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577805.1"
                     /translation="MKDFIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSMYLMIIFL
                     ICLGTASIIYNYKNNR"
     gene            46137..47801
                     /gene="alsS"
                     /locus_tag="POB92_000280"
     CDS             46137..47801
                     /gene="alsS"
                     /locus_tag="POB92_000280"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDI6577806.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEP
                     FINQQHLDELEQLRAHIDEETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            47838..48542
                     /gene="budA"
                     /locus_tag="POB92_000281"
     CDS             47838..48542
                     /gene="budA"
                     /locus_tag="POB92_000281"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI6577807.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(48929..49354)
                     /locus_tag="POB92_000282"
     CDS             complement(48929..49354)
                     /locus_tag="POB92_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6577808.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(49543..51990)
                     /locus_tag="POB92_000283"
     CDS             complement(49543..51990)
                     /locus_tag="POB92_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDI6577809.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSIDKYQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFEATENEAKKLLSEMKTESDRKYLWE
                     NSKDLDTKSADMTRTYRNIEKIAEAMKHKDTKLKIDENKKKVKDALEWLHKNAYGKEP
                     DKKVADLTSNFKNKTSRNTNLNWWDYEIGTPRALTNTLILLNDQFSNDEKKKYTAPIK
                     TFAPESDKILSSVGQPEQAKGGNLVDIAKVKLLESIIEEDKDITKNSIDAFNKVFTYV
                     QSNATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLSDAMDDSTK
                     AKYKQIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSVF
                     LESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSNT
                     DSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSDSTQTFIIN
                     NTKVEVKAKGMFILKKKDDKTYECSFYNPESTNTASDIESKISMTGYSITNKNTSTSN
                     ESGVRFELQQTLNKDDN"
     gene            complement(51987..52154)
                     /locus_tag="POB92_000284"
     CDS             complement(51987..52154)
                     /locus_tag="POB92_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577810.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
BASE COUNT        18528 a         7906 c         9819 g        15929 t
ORIGIN      
        1 ataaatatca atcttatgca aatttgacaa ttgtttgaat caatatataa acaggcaacg
       61 gttcttttca aatataatag taagtgtata atgaaaatgt aaatattatt aaaaaagggg
      121 gttcactcaa tgaaaatgaa acgttttata gctattgtaa tggcattatt tttagtatta
      181 gctggttgct ctaattctaa cgataataat gaaagtaaaa aagatgacgc agacaatggt
      241 aagaaacaag agattcaagt tgcagcggca gcaagtttaa cagatgtaac caagaaatta
      301 gcttcagaat ttaaaaaaga gcataaaaat gctgatatta aatttaacta tggtggatca
      361 ggggcattaa gaaaacaaat tgaatcaggc gcacctgttg acgtatttat gtctgcaaat
      421 actaaagatg tagatgcatt aaaagacaag aataaagcgc atgatacata taaatatgcg
      481 aaaaatagtc tagtattaat tggtgataaa gattcaaatt acacttcagt aaaagactta
      541 aaagacaatg ataaattagc attaggtgaa gtgaaaactg taccagcagg aaaatatgcg
      601 aaacagtatt tagataacaa taacttattt aaagaagtcg aaagtaaaat cgtttatgct
      661 aaagatgtaa aacaagtatt aaattatgtt gaaaagggta atgcgaaaca aggttttgtg
      721 tataaaactg acttatataa acaaaacaaa aaaattgata ctgtaaaagt aattaaagaa
      781 gtagaactta agaaaccaat cacatacgaa gctggtgcta catcagatag taaattagca
      841 aaagagtgga tggaattctt aaaatcagat aaagctaaag aaatattaaa agaataccac
      901 tttgcagcat aaggagttgt aatccatgcc tgacttaaca cctttttgga tatcaatacg
      961 agttgctgta atcagtacga ttattgtaac ggttttaggt atttttatat ctaaatggtt
     1021 gtatcatcgt aagggttcgt gggttaaagt attggaaagt ttattgatat tacctattgt
     1081 tttgccgcca acggtattag gttttattct attaatcatc ttctcgccaa gaggaccaat
     1141 cggtcaattc tttgcgaatg tactacattt acctgtagtg ttcactttga caggtgctgt
     1201 gatagcatct gtcattgtta gttttccact aatgtatcaa catactgtgc aaggcttcag
     1261 aggtatagac acgaaaatga ttaatacagc tagaacgatg ggagcaagtg aaacgaaaat
     1321 tttcctcaaa ttaattttac cattagctaa acgctctatt ttagcaggta taatgatgag
     1381 ttttgctcgt gcattaggtg agtttggtgc tacattaatg gttgcaggat atattccaaa
     1441 taaaacgaat acactacctt tagaaatata cttcttagtg gaacaaggta gagaaaatga
     1501 agcgtggtta tgggtattag tgctagtcgc attctctatt gtggttatat ctacaattaa
     1561 tttattgaat aaagataaat ataaggaggt cgactagatg cttaaaatca atgtgaaata
     1621 tcaattaaag aacactttaa ttcgcatcaa tatagatgat actgaaccaa aaatttatgc
     1681 agttcgtggt ccatctggca ttggtaaaac tactgtttta aatatgattg ccggattacg
     1741 taaagcagat gaagctatta tcgaagtgaa tgggcaatta cttactgata cggcaaaaaa
     1801 cgtgaatgtt aaaattcaac aacgacgtat tggatatctg tttcaagact accaattgtt
     1861 tcctaatatg acggtctata aaaatattac ttttatggct gaaccatctg aacacatcga
     1921 tcaattaatt caaactttaa acattgatca tttgatgaaa caatatccta tgacattgtc
     1981 aggtggagag gcacaacgtg tagcacttgc acgtgcactt agcacgaaac cagatttaat
     2041 tttattagat gaaccttttt ctagtttgga tgatactaca aaagatgaga gtattacatt
     2101 agttaaacgt attttcaacg aatggcaaat accaatcata tttgtgacac attcaaacta
     2161 tgaagcagaa caaatggctc atgaaattat tacaattggg taatcattta tttgccatta
     2221 aagagtttag aacgtattta aaattgtaga agtgaatgct tctatcagca ttttaatgat
     2281 gttttaaact cttttttagg ggcagttttt ttgagagaca ttgacgcgcg tcatataatg
     2341 aaagtaatga taaaaagaaa ggataactta atgtgagtca agaacgttat tcaaggcaaa
     2401 ttttatttaa acaaataggt gaaataggtc aaagcaaaat aaatcaaaaa tgtgcgttga
     2461 ttattggtat gggcgcatta ggtacacatg tggctgaagg acttgttaga gcaggcattg
     2521 ctaaactaat cattgttgat agagattata ttgaatttag taatttacaa agacaaacat
     2581 tgtttactga agaagatgct ttgaaaatga tgcctaaggt ggttgcagct aaaaagcatt
     2641 tgctagcgtt acgtagtgat gttgatattg atgattatat tgcccatgtg gattattatt
     2701 ttttggaaac acatggacag gacgttgacg ttattattga tgcaaccgat aactttgaaa
     2761 cacgacaact gattgatgat tttgcatata aacatcgtat accttggatt tatggtggtg
     2821 ttgtacagag tacatataca gaagctgcat ttatacctgg taaaacacct tgctttaact
     2881 gtttggtacc acaattgcca gcattaaatt taacatgtga tacggtaggg gtcattcaac
     2941 ctgccgtgac gatggcaaca agtttacaat taagagatgc gatgaaagta ttaacggaac
     3001 aaccaattga cacaaaaata acttatggcg atatttggga tggtagtcat tattcatttg
     3061 gtttcagtaa aatgcaacgt tcagactgta caacttgtgg agatgtacca agttatccgt
     3121 atttaaacaa gaatgaacaa cgttatgcaa cattgtgtgg tagagacact gtacagtatg
     3181 aaaatgcatc aattacacac gacattcttg ttcaattttt aaaacaacat caactaaatt
     3241 atcgcagtaa ttcgtatatg gttatgtttg aatttaaagg acaccgcatt gttgctttta
     3301 aaggtggaag gtttttaata catggcatga cacgcacatc agatgccaca catctaatga
     3361 atttattgtt tggataaaaa aagataagac aaaaggagtg taatattatg ggcgaacatc
     3421 aaaacgttaa attgaatcgt acagttaaag cagccgtact aacggtatca gatactagag
     3481 actttgatac agataaaggt ggtcaatgcg tgcgccaact attacaagca gatgacgttg
     3541 aagtgagtga cgcacattat acaattgtga aagatgaaaa agtagccatc acgacgcagg
     3601 tgaagaagtg gttagaagaa gatattgatg tcatcattac gactggtgga acaggtattg
     3661 cacaacgtga tgtgacgatt gaagcagtaa aaccactttt aactaaagag atagaaggct
     3721 ttggggaatt gtttagatat ttgagttatg ttgaagatgt tggcacgcgt gcattattgt
     3781 ctcgtgctgt agcaggtaca gttaataata aattgatatt ttcgattcca ggatcaacag
     3841 gcgcagttaa attagcatta gaaaagctca ttaaaccaga attaaatcat ctgattcatg
     3901 agcttacaaa ataatttatt gatttgattg gcgttgaaaa tctccagatt taccgccaga
     3961 cttgctttca aggtaggttt cgccaataat cataccttta tcaactgctt tcgtcatgtc
     4021 gtaaatggtt aaagccgttg ctgatgcagc ggttaaagct tccatttcaa caccggtttt
     4081 gccagttgta gagacagttg tttgaatgtt taaagtataa aggggtgcat ttgtttcatc
     4141 ccagctgaag tgaacatcta tgccagtcaa tggtaatgga tggcacatcg gaataattgt
     4201 tgatgtattt ttggcagcca taataccagc gatttgagca gtgttcaata cattgccttt
     4261 ggtattggtg ttattagaaa tctgcttaaa aatagtttta tttacagtaa tgcttgaatg
     4321 agcgacagca gttctttttg taatttgttt gtctgataca tcgaccattt tggcgtggcc
     4381 ttgttgatta atatgagtaa actcagtcat tttacccctc ctagtgcatc tagtatatca
     4441 tgaaaaaata aaagttttgg agatgatttt taatggtagt agaaaaaaga aacccaatcc
     4501 cagttaaaga agcaattcaa cgtatcgtta atcagcagag ttcaatgccg gcaattacgg
     4561 tagcacttga aaaaagtcta aatcatatct tagcagaaga tattgtagct acttatgata
     4621 taccaaggtt tgataaatca ccttatgatg gttttgcaat tcgcagtgtt gattcacaag
     4681 gggcaagtgg tcagaatcgc attgagttta aagtgattga tcatattggt gcaggttcag
     4741 tttctgataa attagttggg gatcacgaag cggtgcgtat tatgactgga gcacaaatac
     4801 ctaatggcgc agatgctgtt gttatgtttg aacaaacgat tgaactagaa gatacattta
     4861 caattcgtaa accattttca aaaaatgaaa atatatcttt aaaaggtgaa gaaacaacga
     4921 caggcgatgt tgttctaaaa aaaggacaag taattaatcc aggggctatc gcggtccttg
     4981 caacatatgg ctatgcagag gttaaagtta ttaagcaacc gagtgtcgct gttattgcaa
     5041 caggaagcga attattagat gttaatgatg tattagaaga tggaaaaatt cgtaactcta
     5101 atggcccaat gattcgtgcc ttagcagaaa aattaggtct tgaagttggt atttacaaaa
     5161 cacaaaaaga tgatttagat agtggcatcc aagtcgttaa agaagctatg gaaaaacatg
     5221 atatcgttat tacaacgggc ggagtttctg ttggagattt tgactattta cctgagattt
     5281 ataaggctgt aaaggcggaa gtgttattta ataaagtagc aatgcgtcct ggtagcgtaa
     5341 caacggttgc atttgtagat ggaaagtatt tgtttggatt atctggaaat ccatcagctt
     5401 gttttacagg atttgaacta tttgtgaaac cagctgttaa acatatgtgt ggcgcactag
     5461 aagtcttccc gcaaataatt aaagcaacat taatggaaga ttttaccaag gcaaacccat
     5521 tcacacgatt tatacgtgct aaagcaacgt taacaagtgc tggagctact gtagtacctt
     5581 caggattcaa taaatcaggt gcggttgtag cgattgcaca tgctaactgt atggtcatgt
     5641 taccaggagg gtcacgtggt tttaaagcgg ggcatacagt agatattata ttgactgaat
     5701 ctgacgctgc tgaagaggaa cttcttttat gattttacaa attgtaggtt acaaaaagtc
     5761 tggtaagaca acattgatga ggcatattgt ctctttctta aagtcacatg gttatacagt
     5821 tgctactatt aaacatcatg ggcatggtaa ggaagatatt caattacagg attcagacgt
     5881 cgatcacatg aagcattttg aagcgggggc agatcaaagt attgtacaag gttttcaata
     5941 tcagcaaact gtaacacgtg tagataatca aaatcttact caaattattg aaaaatctgt
     6001 tacaattgac accaatatcg tattagttga aggctttaaa aatgctgatt ttgaaaaagt
     6061 cgtagtctat cgaaatgaag aagagttgca agtattacaa caattgtcga atgtttgtta
     6121 tagcattaat gtaagggagc atgaagattt tacagcattt gagcaatggt tattaaataa
     6181 aattaaaaat gattgtgata cacaattaac atagaggatt gaaatgaatg aaacaatttg
     6241 aaatcgtgac agaaccgata caaacagaac aatatcgtga attcactata aatgaatatc
     6301 aaggtgcagt agttgttttt accggtcatg ttcgcgaatg gactaaaggc gtcaaaacgg
     6361 aatatttaga atatgaagcg tatattccaa tggctgaaaa gaaattggca caaattggag
     6421 atgaaataaa tgaaaaatgg cctggaacga taacgagtat tgttcataga atagggccat
     6481 tacaaatttc agatatcgct gtattaattg cggtttcttc accgcatcgt aaagatgcct
     6541 atcgagcaaa tgaatatgca attgagcgta taaaagaaat tgttccgatt tggaaaaaag
     6601 aaatttggga agatggttca aaatggcaag ggcatcaaaa agggaattat gaagaagcga
     6661 agagggagga ataagagaga tgaaggtact ttacttcgca gaaattaaag atatattaca
     6721 aaaagcacag gaagatattg tgcttgaaca agcattgact gtacaacaat ttgaagattt
     6781 attgtttgaa cgttatccgc aaatcaataa taaaaagttt caagttgctg taaatgagga
     6841 atttgtacaa aaatcggatt tcattcaacc taatgatact gttgcattaa ttccaccggt
     6901 tagtggaggt taagggagca tgaaagcaat aattcttgca ggtggtcatt cagtgcgatt
     6961 tggtaagccc aaagcttttg cggaagtgaa cggtgagacc ttttatagta gagtaattaa
     7021 gacattagaa tcaacaaata tgttcaatga aattattatt agtacaaatg cgcaattggc
     7081 aacgcaattt aaatatccaa atgttgttat agatgatgag aatcataatg ataaaggtcc
     7141 attagcagga atttatacaa tcatgaagca acatcctgaa gaagaattgt tttttgtcgt
     7201 ttctgttgat acaccaatga ttactggtaa agctgtaagc acgttgtatc agtttttagt
     7261 ttctcatctt attgaaaatc atttagatgt cgcagctttt aaagaagatg gacgttttat
     7321 tccaacaatt gcattttata gtccgaatgc attaggcgct ataactaaag cactacattc
     7381 tgataattac agttttaaaa atgtatatca tgaattatca acggattatt tggatgtaag
     7441 ggatgtagat gcgccctcat attggtacaa aaatataaat tatcagcatg atttggacgc
     7501 tttaattcaa aaattgtaag ctgttaggag gtccacaaat ggtagaacaa ataaaagata
     7561 aactaggacg tcccatccgt gacttacggt tatctgtgac agatcggtgt aactttaggt
     7621 gtgattattg catgcctaaa gaggtatttg gagatgattt cgtattttta cctaaaaatg
     7681 aacttttaac gtttgatgaa atggctagaa tcgctaaggt atatgcagaa ttaggtgtaa
     7741 aaaaaatacg cattacaggt ggagaaccat tgatgcgacg ggatttagat gtacttatag
     7801 ctaaattaaa tcaaatcgat ggtattgaag atattggttt gactacaaat ggtttgttat
     7861 taaaaaagca tggacaaaag ttatatgatg ctgggctgcg cagaattaat gtcagtttgg
     7921 atgctattga tgatacgcta tttcaatcaa tcaataatcg taatattaaa gcgactacga
     7981 ttttagaaca aattgattac gcgacgtcta ttggtttgaa tgtaaaagta aatgttgtta
     8041 tacaaaaagg tattaacgat gatcaaatca taccaatgct tgaatatttt aaagataaac
     8101 atatagagat tcgatttata gaatttatgg atgttggtaa tgataatgga tgggatttca
     8161 gtaaagttgt aactaaagat gaaatgctta caatgataga gcagcacttt gaaatcgatc
     8221 ctgtagaacc aaaatatttt ggggaagtag caaaatatta tcgccataag gataatggtg
     8281 ttcaatttgg tttgattaca agtgtttcac aatcattttg ttctacatgt acacgcgcaa
     8341 ggctgtcatc agatgggaag ttttacggat gtttatttgc aactgtcgat ggatttaacg
     8401 ttaaagcgtt tattcgttct ggcgtgaccg acgaagaatt aaaagaacaa tttaaagctt
     8461 tatggcaaat aagagatgat cgatattcag atgagagaac tgctcaaaca gttgccaatc
     8521 gtcaacgtaa aaagataaac atgaattata ttggtggtta atgtgtaggg accactacat
     8581 attaaatcat tagagatgtt ttaatatttc tgtcttactc cctaaaatac aatattattt
     8641 attaaagtaa aaacggtcat atctatgcca gatttaatag aaatgatcgt ttttaaagtt
     8701 tttacaagtt ggcggggccc caacacagaa gctgacagaa agtcagctta caataatgtg
     8761 caagttggcg gggccccaac atagaaggtg acgaaaagtc agcttacaat aatgtgcaag
     8821 ttggggaaca acgaaataaa ttttatgaga atatcatttc tatcccactc ttaagaatca
     8881 ctacataata aatctttagt ggttctttaa cattgatgtc acactccatg ccattgagtt
     8941 gtaatatatc ttttttaggt ataaatgttg tcgaataaac aacaagttgt ccaaaagata
     9001 taaatctaaa caagatatag ccagcaattt aatatttgta atagataaaa tgctaagttt
     9061 gatatataat aaatttaagt aattgtataa taatatgaat tacaaacatc taagaagaaa
     9121 cataggaggc atcatattat gagtaataaa gttcaacgtt ttatagaagc agaaagggag
     9181 ttaagtcagt taaagcactg gttaaaaaca acacataaga tttcaattga agaatttgta
     9241 gtacttttta aagtgtatga agctgaaaag attagcggta aagaattgag agatacatta
     9301 cattttgaaa tgctatggga tacaagtaaa atcgatgtga ttatccgtaa aatctataaa
     9361 aaagagctta tttctaaatt gcgttctgaa acggatgaaa gacaagtatt ctatttctat
     9421 agtacttctc aaaagaaatt gttagataaa attactaaag aaatagaagt gttaagcgtt
     9481 acaaactaaa aacttaaaaa gcatgccaat cagtacatca taattgcgtc ttggggacag
     9541 acaaatgatg aatagagatt ggcatgcttt ttaattttga atataaatat ttagttcatg
     9601 gcatttctag ttacatgacg tccatgaatt aagaagtaaa caagcatagt aatgattgct
     9661 aaagcggcca taaagccgaa gatttcacta tatgaaaaca tatgagtaaa taacccaagg
     9721 aatgatggac cgaagccgac acctgcatct agaccaacgt aaaaagtaga tgtcgcgata
     9781 ccatatttaa tcggaggtga gacttttatc gcaatagatt gcattgcaga tgataaattt
     9841 ccatacccta aacctaggca agcaccagca agtaatatta accagctttg atagcttgaa
     9901 attaagcata caaatgaaag gaaaagcatg ataaatgctg ggtagacaat aatattttca
     9961 tttttatcat ccatcaatct accagcaata ggtctagtaa ttaacgatgc tatagcatag
    10021 caaataaaga aatagcttgc tgcagttact aggtgtcgct ctaaagcaaa tgcttgtaaa
    10081 taagttagga tggacgcata ggtaacgcca attaaaagca taattacagc aacaggaatg
    10141 gcctcttttg caataaattg atgaatacta aatcttggtt tatcaatgac attagtttca
    10201 gttttattat ttgttacttc gaaatcaact tttataaata atgagataat gagtccgagt
    10261 atgcctaata tgacacaaat aataaacagt aaatcaattg ggtattttgt aataagtaac
    10321 atgcctagaa atgggccaat cgctgtacct aatactaaac ttaaggaaaa taaactgatg
    10381 ccttcacttt ttctattaac aggggtaacg tatgccgcaa tagtacctgt tgcagttgtc
    10441 acaactgcag ttgcgatacc gtttatgaga cgtacaaaga ttaaaaaagc taaagatcca
    10501 tcaataaaat aaagtaattg cgtgataatt aaagcaatta aaccaataaa taataatcgt
    10561 ttaggtccga tttgatttac aaatttacct gtagcaaatc gaccaattaa agacccgaca
    10621 ataaatagtc ctacaactaa tccggctaat gaatcggaag cgtcgaaagc ttgcttacta
    10681 tagccagcaa taactacaag taacaaatac atgcataggt atacgataaa gttgacaaaa
    10741 aagttgattg taaaactttt ggtgaaaata ggtgatttat ttaatgagcc ttgattagtc
    10801 attgtgtgtc tcctttaata atattttatg aatttgattc atcacattta tggtttcttg
    10861 catttgatct tgcgaaatgt ttgttttggc aactactgct tgttggagct tttgtacatc
    10921 aagacaaaca atctcatata attcatgccc tttaggtgtt aaagttaaaa acttttgtcg
    10981 tttatcctct aaactgtttt caactgttat aaggtcattt tctattagag cttttaaagt
    11041 ttttcttgca gtaggctttt caattgcacg tctatgtgaa atttcaataa gagtagtggg
    11101 ctgatgttta gcgatatcgc gtaagattaa ccattggcca taatatatat tgtgtttttc
    11161 taaaatcggc tcggctaatt ttaaataggg gcgatatatt gttataaaac tattgaaaaa
    11221 ttcttgtgat agcatagtga catctcctaa gacaaaatag ttagcttagc taaccttttt
    11281 acaacaatag taattataaa acgggagcaa ttagaaatca atatataatt attaagagca
    11341 aaaatattta tactttgtta aaataagcgt aattacatgt aaataggggg atactaatga
    11401 tattgaaatt tgatcacatc attcattata tagatcagtt agatcggttt agttttccag
    11461 gagatgttat aaaattacat tcaggtgggt atcatcataa atatggaaca ttcaataaat
    11521 taggttatat caatgaaaat tatattgagc tactggatgt agaaaataat gaaaagttga
    11581 aaaagatggc aaaaacgata gaaggtggag tcgcttttgc tactcaaatt gttcaagaga
    11641 agtatgagca aggctttaaa aatatttgtt tgcgtacaaa taatattgag gcagttaaaa
    11701 ataaactaca aagtgagcgg gttgaagtag taggtccgat tcaaatggaa agagatacac
    11761 ataaagatgg taaggtaaag tggcaattgc tttatataat gaatcaggat gatgatgaaa
    11821 ttaagccacc attttttatt caatgggaag aaagtgattc catgcgtact aaaaaattgc
    11881 aaaaatattt tcaaaaacaa ttttcaattg aaactgttat tgtgaaaagt aaaaaccgat
    11941 cacaaacagt atcgaattgg ttgaaatggt ttgatatgga cattgtagaa gagaatgacc
    12001 attacacaga tttgatttta aaaaatgatg atatttattt tagaattgaa gatggtaaag
    12061 tttcaaaata tcattcggtt atcataaaag acgcacaagc aacttcacca tattcaattt
    12121 ttatcagagg tgctatttat cgctttgaac cattagtata aatatacgta agtgctatga
    12181 gcgagaatgc ccatatgaat aatggcaagc acaatggaaa gaatcgttaa tatattattt
    12241 aatcgtgatg acttaattaa aatgaaaaag attgataata taaatgtgaa aaagataagt
    12301 ataacccgta aactaaagta attcacggtg agaggttgac tcaatgtcat aatgattgca
    12361 acgatgttca taattataaa tagacttaaa ataattgttc tcatatcaaa cacctcattg
    12421 ttagattatt gacattataa caggggtaat tgtatatgaa cattaatgtg gttgcttgag
    12481 gaaaaattta ttcattgaag tcaagttggt tcattttaga aatgaatatc gtgttagatg
    12541 atgaaagtat attgaagtat aggtaactag ttgaaaagta ttaattgtac gataacatta
    12601 aatttaacac gaaacataga tataaaatga ttcacaatta aaatgggtaa atttgaactt
    12661 gctaaactat taattggagc atggacattt caaaaataag agttcaaatc ttacacaagc
    12721 tctgaatcga cactataaga tacaaactgt ataattaaag gtattgttaa atagaaggag
    12781 atatcataaa tcatggaaaa gatgcatatc actaatcagg aacatgacgc atttgttaaa
    12841 tcccacccca atggagattt attacaatta acgaaatggg cagaaacaaa gaaattaact
    12901 ggatggtacg cgcgaagaat cgctgtaggt cgtgacggtg aagttcaggg tgttgcgcag
    12961 ttacttttta aaaaagtacc taaattaccg tatacgctat gttatatttc acgtggtttt
    13021 gttgttgatt atagtaataa agaagcgtta aatgcattgt tagacagtgc aaaagaaatt
    13081 gctaaagctg agaaagcgta tgcaattaaa atcgatcctg atgttgaagt tgataaaggt
    13141 acagatgctt tgcaaaattt gaaagcgctt ggttttaaac ataaaggatt taaagaaggt
    13201 ttatcaaaag actacatcca accacgtatg actatgatta caccaattga taaaaatgat
    13261 gatgagttat taaatagttt tgaacgccga aatcgttcaa aagtgcgctt ggctttaaag
    13321 cgaggtacga cagtggaacg atctgataga gaaggtttaa aaacatttgc tgagttaatg
    13381 aaaatcactg gggaacgcga tggcttctta acgcgtgata ttagttactt tgaaaatatt
    13441 tatgatgcgt tgcatgaaga tggagatgct gaactatttt tagtaaagtt ggatccaaaa
    13501 gaaaatatag cgaaagtaaa tcaagaattg aatgaacttc atgccgaaat tgctaaatgg
    13561 cagcagaaga tggaaacatc tgaaaagcaa gctaaaaaag cgcaaaatat gattaatgat
    13621 gcgcaaaata aaattgctaa aaatgaagat ttaaaacgag acctagaagc tttagaaaag
    13681 gaacatcctg aaggtattta tctttctggt gcactattaa tgtttgctgg ctcaaaatca
    13741 tattacttat atggtgcgtc ttctaatgaa tttagagatt ttttaccaaa tcatcatatg
    13801 cagtatacga tgatgaagta tgcacgtgaa catggtgcaa caacttacga tttcggtggt
    13861 acagataatg atccagataa agactcagaa cattatggat tatgggcatt taaaaaagtg
    13921 tggggaacat acttaagtga aaagattggt gaatttgatt atgtattgaa tcagccattg
    13981 taccaattaa ttgagcaagt taaaccgcgt ttaacaaaag ctaaaattaa aatatctcgt
    14041 aaattaaaac gaaaatagat taacgactga aatctgaacg ctcataagac tgtcatttgc
    14101 gttcagattt ttttacacaa tatagaatgg ttgagtaaaa tatttttgaa tatagtgaaa
    14161 gagggggaag tactgtgata aaaaagctat tacaattttc tttagggaat aagtttgcta
    14221 tctttttaat ggttgtttta gttgtcttgg gcggtgtata tgcgagtgct aaattgaaat
    14281 tagaattact accaaatgta caaaatccag ttatttcagt tacaacaaca atgccgggtg
    14341 caacgccaca aagtacccaa gatgaaataa gtagtaaaat tgacaatcaa gtaagatcat
    14401 tggcatatgt gaaaaatgtt aaaacgcaat ccatacagaa tgcttcaatt gtaacagttg
    14461 aatatgaaaa taatacagat atggataaag cagaagaaca gcttaaaaaa gaaatcgata
    14521 aaattaaatt taaagatgaa gttggtcaac cagaattaag acgtaattcg atggacgctt
    14581 ttccggtttt agcatattca ttttcaaata aagagaatga cttgaaaaaa gtaacgaaag
    14641 tactgaatga acaattaata ccaaaattac aaacggtaga tggtgtgcaa aatgcgcaat
    14701 taaatgggca gacgaaccgt gaaatcaccc ttaaatttaa gcaaaatgaa cttgaaaaat
    14761 atgggttgac tgctgatgat gtagaaaact atctaaaaac ggcaacaaga acaacaccac
    14821 ttggattgtt ccaatttggt gataaagata aatcaattgt tgttgatggt caatatcaat
    14881 ctgttgatgc ttttaaaaac ataaatattc cattaacgtt tgcaggagga caagggcaat
    14941 ctcaatccca aagtgacaat aaacaaaatt cagccatgtc agacgttaat caggcatcac
    15001 cacagcaaaa ttcaaaagcg tcagcaccaa ataatataag tggtatgccg acagcgaaac
    15061 taggagattt agctgatatt acagttggtg atgtgcgtac ttctatttct aaaacgaatg
    15121 gaaaggatgc ggttaatcta caaataacta aagctcaaga tgccaataca gttcaagtag
    15181 ccaaagatgt acaacgtaaa attgatacat tcgttgatga aaataaagat tttaatgtca
    15241 caaaaacaat ggatactgca aagcctgttg agaaatcact ttatacgatg gttgaaaaag
    15301 catcattagg tacaatcgtg gcaattatag ttattttgct gtttttaaga aacattcgta
    15361 cgacggcaat ttctattata tccattccgt tatcacttct tatggcgctt attgctctga
    15421 aattgagtga tgtttcattg aatatactaa cgttaggtgc attaacagta gcgattggac
    15481 gtgtgataga cgattcgatt gtagttgttg aaaatattta tcgacgctta acagattcag
    15541 aagaacaatt aaaaggtgaa aatttaatta tcagtgcgac aactgaagta tttaaaccaa
    15601 taatgtcatc gacactagtt actattatcg tcttcttacc acttgtgttt gtatcaggtt
    15661 cagtaggcga aatgtttaga ccttttgcat tggctattgc atttagttta ttagcatcgt
    15721 tattagtgtc aattacactc gttccagctt tggcagcaac actatttaaa aaaggcgtta
    15781 aacgtcgtaa taaacaacat caagaaggat taggtgttgt tagtacaact tataaaaaag
    15841 tattgcattg gtcactaaat cataagtgga ttgtaattat attaagtact ttaattttgg
    15901 ttgtaactat tgtatttgga ggaccgagac taggcactag ctttatttca gcaggtgacg
    15961 ataaattttt agctattact tatacaccga agcctggtga aacggagcaa gcagtgttga
    16021 atcatgcgaa agatgttgaa aaatatttaa aacagaaaaa gcatgtaaaa acaattcaat
    16081 actcagttgg tggtagtagt ccagtagatc caacgggtag tacaaatagt atggcaatca
    16141 tggttgaata tgataatgac acgcctaatt ttgatgtaga agcggataag gttattaaac
    16201 atgcagatgg ctttaaacat cctggagagt ggaaaaatca agatttagga acaggtgcag
    16261 gtaataaatc tgtagaggtt actgtaaaag gtccatcaat ggatgccata aaatcaactg
    16321 taaaagatat tgaacagaaa atgaaacagg ttaaaggact agccaatgtc aaatctgatt
    16381 tatcgcaaac atatgaccag tatgaaatta aagttgatca aaataaagcg gcagaaaatg
    16441 gtatttctgc aagtcaactt gcaatgcact tgaatgaaaa cttaccagaa aaaacagtta
    16501 cgactgttaa agaaaatggt aaaactgttg atgttaaagt caaacaaaat aagcaaacag
    16561 actggtcaga agataagttg aataatataa ctttgaaaaa gccgactggt ggtacgatta
    16621 aattgggaaa tatcgctacg ttagttaaaa caacgacacc aagtaaattg acgcaagaac
    16681 aaggagatta tgcaacgacg gtatctgcta aagtaacaaa taaagatgtg ggtggcacaa
    16741 cacgacaagt gatgtctaaa ataaataatt tggacaaacc gaataatgta aaggttaata
    16801 tcggtggtgc atcagatgat attaacaatg caatgacaca attagccttt gcaatgttag
    16861 ctgcgatcat tatcgtatat ttaatcctag ttattacatt taaaggtggc ttagcaccat
    16921 ttacaattct attctcttta ccatttacag ttatcggtgt aattattgca ctattaatca
    16981 caggagaaac aatatcagta ccaagtttaa ttggtatgct aatgttaatt ggaatcgtag
    17041 taacaaatgc cattgtgtta atagaccgtg tcattaataa tgagcagcag ggcatggaga
    17101 tgaaagaagc attaatcgaa gcaggcggta ctagaattag accgatatta atgacggcaa
    17161 ttgcaacaat cggtgcatta gttcctttgt tatttggtca agatagctcg attcttattt
    17221 cgaaaggatt agccgctaca gttattggtg gtttaatttc atcaacttta ttaacacttg
    17281 tagttgtacc agtaatatat gaaattctct ttactttgaa aaaacgattc actaaacgat
    17341 agataatata aaagccataa aagcggtcat gatattgact acttttatgg ctttttaaaa
    17401 tttgaaattt tagatcttat ttcacgtgcg atttaatcga tgtgttaata aaacaatgct
    17461 ggcattcaac atgtacgata ataaaataaa attgcaatta tgttattgca tgttttgttt
    17521 attacttaga ataatacgat ggttaagatg aaatatatga caaagataaa taatgacatt
    17581 aaattcaaga tgtttacacc ttttagatct tttttaagca ttttaaatcc tgtcacttcg
    17641 acattaccga ctaagagtaa taagacgata aatacccacc aattctcttt tggaaaactc
    17701 atcatcgtta atgctaagac gaataataac aacacaccca taataattct caaaatgcga
    17761 gtaaatacaa cttccatttt aaaaatacta ataaaactaa caattagata aagtattgct
    17821 agtaaaatca gataaaattg catatgataa cttcctctcc gtttcaaagg gttcatgtag
    17881 tacagcagcg tagaaaacat aaatagcagt gttgggatag atatgcgtgt atgttgttaa
    17941 tgaaacacta aatcataaca cgacaacatc ttgtgattct agatgtaatt gagcgaaaaa
    18001 taaagcaatt catcaatata acgtattcag agcaatgcta gtcaaattta tgtaacgaaa
    18061 agcatttatt gcttgaatga tagattatta atcttgcatt tgagcgcgaa cgtttctaat
    18121 ttcattttca atatctttaa gttgacgaac aagtgcggaa gtatctaaat tcaaagattc
    18181 ccgtttttct aagtcacctt gaagacgaac ataatcatat ttaagttcgg ctagttggtt
    18241 ttctaaagac atactataac actcctttga aatataatta agtgcattta atgtagatta
    18301 tagcatgaaa cagtagactt ctaaaatatc tgtggagtat gttaaagtta taaattatga
    18361 agattattga aagaagggat aacagtgaca gaacaattta ctatgattat tttattaatt
    18421 gcgcttggtt actttttgaa gcgcattaat tttataaaag caacagatag tcaagtcatt
    18481 gctacattag ttcttaatgt cacactccca tctttagtta ttgtgaattt aaacagtgca
    18541 gaattaaaat tgtcattctc cattttacca attttaatga ttatatatgg tattgtggcg
    18601 aaaatgattg ctgtctggtt ttttagaaaa tatgataatc acatgcgtgg atcagtaggt
    18661 atgatgactg gtgctatgaa tattggattg tttgcatatc cactaattga agtgatatgg
    18721 cctaaaacgg gacttattta ttttgttatg gctgatatag gtggtgcctt tgtgatgttt
    18781 ggaattactt actttgtagg tagctacttt agtgaaggta gtgatcaatt cgattttaaa
    18841 tatttaggta agaaattatt acaatcagtg ccactcgtta catacattgt aatgtttata
    18901 ttgaatataa gtcacatcca tttaccacat gtggcaatcg atttctttag catactttcc
    18961 catgcaaata tgccgttatc aatgattttg ctaggtgtca tgttgaattt tactctggaa
    19021 agaaagtata tacctgctac tattaaatat ttatgtttgc attatggatt agcattagtt
    19081 gcgggattgt tagtatatgt gttgttacca gtatcagatg atatgattaa gacgacattg
    19141 ttggtcactt ggatgttccc ggtaggtgtt gcaattattt cttatggtat tcagtttaaa
    19201 tatcgaacac tgccatttat aggtatgacg accaatttga caatcattat tagtatcatt
    19261 attttgtacg tgtatcaggc agtgtttgtc tagtttacac atttgaaaat aaaatccatt
    19321 ttagtattcg tagaaaatga gagccccctt taccacaata tgtaaatgta tattgtgaaa
    19381 agggggcttt tatttatgac atagttgttc aactcataca ttcaagttga taatgcagtg
    19441 cgttatgcag ctatccactt catatgtatg gcgcatactt gatatatgaa tatcatcaag
    19501 attttaaaag tatcgctaat tttaaagtct taaaacgcat gattaatgtc atgatgtgtt
    19561 attgcgtttt gtttttagag ccacgtattt cctaggacat atagaggaac attcaacgta
    19621 aaattcccga aaattaattt taatcattct ataagtgaat ggttattttc agaagtgatt
    19681 acatttttac gttacatgag tatttaactt tctatttcaa attacctaga attatagcag
    19741 ctatcacgat aataataata cctgcccaaa tacccgtcat ctgacgacga tcttttcttt
    19801 ctcctaaaat gaaaataccg cctaaggttg aaacgataac aagtaactgt gataatgaga
    19861 aacttgtagc tacaccaact tttggttgag aatagaacat gaacaagtta ccaattcccc
    19921 aaatcacacc tggcaataga ttaagtgctg ttgatttaag tgatgtttta tgattcatgg
    19981 ataggataaa gccaccaatt gccataccga cagattggaa gaacaatgca tcagttccac
    20041 caacaccaaa gatgtcacca agtacaacga aacctacata tccaactgta gatacaatta
    20101 aaatacccat tgctttttta aattcaggat tatctgattg acgttcattt ttagctttaa
    20161 gtgaagtaag tgctacacca gtaactaata atatcatggc gattaaacca aagataattt
    20221 gagtcattga agaccattca cctaaaaaga taacgctgaa taatgttgta ccaactaatt
    20281 gcatacctgt agaaactggc atagtatttg atacaccgat taaactaata gatttaagct
    20341 gattcgcttg tccaaacgcc cataatgcac cagaaataag accgacaata attacagtag
    20401 gattattgaa ttttgcgaaa ccagtaataa gtaaacctaa tccaacaatg agtgcgccta
    20461 gcgtcgtacc acgaatttgg ttgtaaggtc caccgccgac gaacacatta ataagaacga
    20521 cacttcccca gaataaagca ggtaaaagtg cgattaagaa atcaagaaat tgcaagacta
    20581 ctcactctcc tatactattt atgcaagtgc tccccttaat tgacacttgc atgttattgt
    20641 aaagcattct gccctaatag taaaaaacaa ctacttataa ttttcaatat ttacaaaaag
    20701 tagaaaaacc cacaatacat gctaatgatg tactgtaggg tatttgcttg gtgttttatt
    20761 cagggattgt ttgatctttt acgttacgtg attcgaattt gaatttaatg tactcatagt
    20821 tgatatggaa acctagacca ttaaatcgtt cgatgagttt attattttta ttacgaattt
    20881 taaaataaac ttttctaata tcaaaattat caaaagcata ttgcaatgca tacgcaagca
    20941 actcgaaagc gatacctttc aaacggtaat ctacatgaga actgaagtat ttaatctcgg
    21001 cgattgactg ttgtgaatca atttcaaggt ataaataacc tttaagtaaa ccttcgctaa
    21061 caaataaaaa caaatgatga tggtcgtcta aactatcaat gatatcttgt ggtgactgtg
    21121 cctgatattt aaatgtagtt gtgtgtaatt tgctgaaagc acgataaaac cctttgtgat
    21181 aaggaatgat atttggttgg tgcatatctt cttctaatct tgtacgggct tctatgtaat
    21241 agtcagtgaa gttataactt gcttgaataa ctttcattaa tggtttgtat tgttgaatgc
    21301 cttcttcaaa agaaaagtta aagacggcat cgtcaggttg gttcgaagtc attgctttaa
    21361 atagcgtttc aaacgtatcg acagataata cataattgtc agccacgaaa gggcctatga
    21421 ctttgaattt atttttatcg tatgcaaaag acaatatgat tgctttgatt gaatcgtttt
    21481 ctttaatcga aaatacacca ggtgattcaa tcgcatcggt tattaataca tctatttcat
    21541 tgtactgttg aggaagttta tataagtatg atgctgattc ataattcgaa tgttcaatga
    21601 acgattttat ttgtgtaata tcatataact gaactatgct catgtcatta cctccgtact
    21661 ttttgttact tttattatat agtatttcaa ctgaaatgaa agttaatagt gatattaaca
    21721 tgttacaata catttaacac atcatttgca atcattgaat ttaaatcaaa gattagtgga
    21781 atagatgaag cacgttcgaa ataaaagaac gtatgagaaa ggataattta tgaaatcttt
    21841 aatattagct gaaaaaccat cagtcgcaag agatattgct gatgctttac aaataaatca
    21901 gaagcgtaat ggttactttg aaaataacca atatattgtc acgtgggcgt taggtcatct
    21961 agtgacaaat gcgacacctg aacaatacga taaaaattta aaggaatggc gattagaaga
    22021 ccttccaatt atacctaaat atatgaaaac tgttgttatt ggtaaaacaa gcaaacaatt
    22081 taaaacagta aaagcgttaa ttttagataa taaagtgaaa gatattatta ttgcaacaga
    22141 tgctggacga gaaggtgaac tagttgcaag attgattttg gataaagttg gtaacaaaaa
    22201 gccaatccgt cgattatgga ttagctcagt tactaaaaaa gctattcaac aaggttttaa
    22261 aaatttaaaa gacggtcgtc aatataacga tttgtattat gcagcgttag cgagaagtga
    22321 ggcagattgg attgttggga ttaatgcaac gcgtgcacta acaacaaagt atgatgcaca
    22381 gctatccctg ggacgtgttc agacaccaac gattcaatta gtaaatacac gacaacaaga
    22441 gattaatcag ttcaaaccac aacaatactt tacattatca ttaacggtaa aagggtttga
    22501 ttttcagcta gaatcaaatc agcgatatac caataaagaa actttagaac agatggttaa
    22561 taatttgaaa aatgtcgatg gtaagattaa atctgttgct actaaacata agaagtcgta
    22621 tccgcaatca ctgtacaatt taacagattt acaacaagat atgtatagac gttataaaat
    22681 tggacctaaa gaaacattga atacacttca aagcttatat gagagacata aagtcgtaac
    22741 ctatccaaga acagattcaa actatttaac aactgatatg gtagatacta tgaaagaacg
    22801 tattcaggcg acgatggcaa caacatataa agaccaagca cgcccattaa tgtctaaaac
    22861 attttcatca aaaatgtcga tatttaataa tcaaaaagta tctgatcacc atgcaattat
    22921 tcctacagaa gtgagacctg tcatgtcaga cttaagtaat agagaattaa agttatacga
    22981 tatgattgtc gagcgttttt tagaagcttt aatgcctccg cacgagtatg acgcgataac
    23041 tgtaacttta gaggttgcag ggcacacatt tgttttgaaa gagaatgtaa caactgtttt
    23101 aggttttaaa tctattagac aaggtgaatc tattacagag atgcaacagc ctttttcaga
    23161 aggcgatgaa gtgaagattt caaaaacaaa tattagagaa catgaaacaa cacctccaga
    23221 atattttaat gaaggttcgt tattaaaagc gatggagaac cctcagaact ttattcaatt
    23281 gaaggataaa aaatatgcgc aaactttaaa acaaacaggt ggtatcggca cagttgcaac
    23341 aagggccgac attatcgata aattatttaa tatgaatgcc attgaatcaa gagacggtaa
    23401 aattaaagta acgtcaaaag gtaaacaaat attagaatta gcaccagaag aattaacgtc
    23461 gccactttta actgcacaat gggaagaaaa attactttta attgaacgtg gtaaatatca
    23521 ggcgaaaaca ttcattaatg aaatgaaaga ttttacgaaa gatgttgtaa atgggattaa
    23581 aaatagtgat cgtaaatata aacacgataa tttaacaacc acagaatgcc caacgtgtgg
    23641 taaattcatg attaaagtta aaactaaaaa tggtcagatg cttgtgtgcc aagatccatc
    23701 ttgtaagacg aaaaagaatg tacagcgcaa aacaaatgca agatgtccaa actgtaaaaa
    23761 gaaattaacg ttgtttggta aagggaaaga agcggtatat cgttgtgttt gtggacattc
    23821 tgaaacgcaa gcacatatgg atcagcgtat gaagtctaaa tcctctggta aagtatctcg
    23881 taaagaaatg aaaaagtata tgaataaaaa tgaaggttta gacaataatc cgtttaaaga
    23941 tgcattaaag aacttgaatt tatagataaa atcgaacaaa gttgaatcag aaaaacgaaa
    24001 agttcgcttt tggtattgtt ttttattaag aatgatatta aactattaag gtattttaaa
    24061 aaaaggagca tccattcgtg aaaaactatt tccagttcga taaatatgga acaaacttta
    24121 aaagagaaat cttaggcggt atcacaactt tcttatctat ggcctatatt ttagcagtta
    24181 acccgcaagt tttaagttta gcaggtgtta aaggcgtatc agaagatatg aaaatggacc
    24241 aaggtgccat ttttgtagcg actgcattag cagcatttgt aggctcgcta ttcatggggc
    24301 taatagctaa atatccaatt gcgttagcac caggtatggg attaaatgca ttctttgcat
    24361 ttactgttgt gttaaccatg ggtattcctt ggcaagttgg tttaacaggc gttttattct
    24421 caggtatatt ctttgcgata ttaaccgtca cagggtttag ggaagttatt attaatgcaa
    24481 ttccttatca aatgaagatg gctgtttcag caggtatagg attattcatt acatttgtag
    24541 gtttacaaag ttcgggtatt atagttaaaa atgaatctac attagtaaca ttgggacatt
    24601 taactgatcc accagtactt ttggcgatat tcggtatcgt tattacagtt attttatatg
    24661 caattaaatt gccgggttct atctttatcg gtatgatcat cacagcaatc gttggtatgt
    24721 tcactggatt gattcaaatg ccatctggta ttgtaggtaa gattccaagt attgaaccaa
    24781 cgttcggtgc tgcatttgaa gcgtttaaag acccaagtca attattaacg atacagttct
    24841 taattgttat tttaacgttc ttatttattg atttctttga tacagctggt acattagtag
    24901 ctgttgctac tcaagcaggt attatgaaag ataataaatt accaagagct ggtagagcat
    24961 tgttctcaga ttctttagct acgattgtag gatcagtttt tggaacaact acaacaactt
    25021 cttatatcga atcaacttca ggtgttgctg taggtgctag aacaggattt gctagtatcg
    25081 taacaggttg ttgtttccta ttagcgctgt tctttagtcc gttaatcgct gttgtaacaa
    25141 gtgcagttac gacaccagct ttagtggtgg taggtgtatt aatggctgca aactttgctg
    25201 agataaactg gaaaagtttt gaagttgcag tgccagcatt cattacaatc attatgatgc
    25261 ctttatcata ctcaattgca actggtattg cgtgtggatt tattttctat ccaattacaa
    25321 tgcttatctc taaaaaacat aaagaagtac atccgataat gtatgtatta atggtactat
    25381 tcattctgta cttcatattt gttcatggtt aaatccagac acaaaaaacg actgccaatg
    25441 attggtagtc gttttttctt tgaagtaagg ataaaataag aagcgaaata acataaagca
    25501 atattatgaa tgtctttaaa tatccgatat aaatatactt cttttaaaat tgttggacgt
    25561 gtttcttcta tattatatac ctgattaaaa aggttttaag aattattctc tatgtccatc
    25621 tttgtattgt gtgtaaatga cacgagcatt tttattaggt atataggttg gcgctgataa
    25681 tacaactagg acaaatagaa tcattaggaa aaataaaatc caatgaatca aaattcctaa
    25741 gaaggggatg atagctaaaa aactacctac tataccaaat attggaataa tcgctatagg
    25801 tctaatcgca ttttgtctat tcactaataa tataatagta agaattagat aaagtaatgc
    25861 attaaagatt aaaggctgcc atccaaaact taaaataaac gttgcaccta aaaaaggaat
    25921 gccatagaaa aactcacaaa gtattaaaaa gatacctata agtgctaaag ataattttgg
    25981 tccatttttc atattcattc accaccgttc ttatgactaa ttatatagaa taattttcaa
    26041 tgataataat aaaatgattc aaggtacgaa tttacaataa agaaggaaat aaaaaatatc
    26101 tcgaaaatag ttgaactgac taagtatata aagtaaaata taaaagtatg tgttagacag
    26161 atagaaaaat tatgtgaaaa gccttgattt atcgatgttt ttcgtgtaat ataactaacg
    26221 ttggacttta agaaaagcga tgaagcgaaa ggttactgac acacccggcc gctttgccat
    26281 ggcgctgtgt aagatagttt tcgtggagaa gtctatcact aaatgtagac gaataaggag
    26341 ggaaaattat ggcaaaacaa aaaatcagaa tcagattaaa agcttatgat caccgcgtga
    26401 ttgatcaatc agcagagaag attgtagaaa cagcgaaacg ttctggtgca gatgtttctg
    26461 gaccaattcc gttaccaact gagaaatcag tttacacaat catccgtgcc gtgcataagt
    26521 ataaagattc acgtgaacaa ttcgaacaac gtacacacaa acgtttaatc gatattgtaa
    26581 acccaacacc aaaaacagtt gacgctttaa tgggcttaaa cttaccatct ggtgtagaca
    26641 tcgaaatcaa attataatag acaattttag gaggtggact ttcgatgacc aaaggaatct
    26701 taggaagaaa aattgggatg acacaagtat tcggagaaaa cggtgaatta atccctgtaa
    26761 cagtagtaga agctaaagaa aatgttgtat tacaaaagaa aactgtagaa gttgatggat
    26821 acaacgcaat ccaagttgga tttgaagaca aaaaagcata caaaaaagat gcaaaatcta
    26881 ataaatatgc taataaacca gctgaaggtc acgctaaaaa agctgacgca gcacctaagc
    26941 gcttcattcg tgaattccgc aatgtagacg tggatgctta cgaagtaggt caagaagtct
    27001 cagtagatac ttttgtagct ggcgacgtta ttgacgtaac aggcgtatca aaaggtaaag
    27061 gtttccaagg tgcaattaaa cgccacggac aatctcgtgg acctatgtca cacggttctc
    27121 atttccacag agcaccaggt tctgtaggta tggcttcaga tgcttctaga gtatttaaag
    27181 gccaaaaaat gccaggacgt atgggtggaa acactgtaac tgttcaaaac ttagaagtag
    27241 ttcaagttga cacagaaaac aaagttatct tagtaaaagg taacgtacct ggacctaaaa
    27301 aaggtttagt agaaatcaga acttcaatta aaaaaggtaa taaataataa gaagtgaaag
    27361 gaggaaatgc ataatggcta attatgatgt tttaaaatta gacggaacta aatcaggttc
    27421 aatcgaatta agcgatgcag tattcggtat tgagccaaat aatagcgttt tattcgaagc
    27481 tattaattta caacgtgctt cattacgtca aggtacgcat gctgttaaga atcgttcagc
    27541 agtaagcggt ggcggacgta aaccatggaa gcaaaaagga acaggtcgtg ctcgtcaagg
    27601 tacaatccgt gctccacaat ggcgtggcgg tggtatcgta ttcggaccaa ctccaagaag
    27661 ttatgcatac aaaatgccta agaaaatgcg tcgtttagct ttacgctcag cattatcttt
    27721 caaagctcaa gagaatggct taactgtagt tgacgcattc aacttcgaag ctccaaaaac
    27781 taaagaattc aaaaatgtat tatctacatt agaacaacct aaaaaagtat tagtagttac
    27841 tgaaaacgaa gatgtaaatg ttgaattatc agcacgcaac atccctggcg ttcaagtgac
    27901 aactgctcaa ggtttaaatg ttttagatat cactaatgct gacagcttag taattactga
    27961 agctgctgct aaaaaagttg aggaggtgct cggataatgg aagcaagaga tattcttaag
    28021 cgccccgtaa tcactgagaa atcttctgaa gcaatggctg aagacaaata cactttcgac
    28081 gttgatactc gtgttaacaa aacacaagta aaaatggctg ttgaagaaat cttcaacgta
    28141 aaagttgcaa gtgttaatat catgaattac aaacctaaga aaaaacgtat gggccgttac
    28201 caaggctata caaacaaaag aagaaaagcg attgtaactc ttaaagaagg atcaatcgac
    28261 ttatttaact aataaaacaa ttaccattaa ggaggtaagc gacaatggct attaaaaagt
    28321 ataagccaat aacaaatggt cgtcgtaata tgacttcgtt agatttcgca gaaatcacga
    28381 aaactacacc tgaaaagtca ttattaaaac cgctaccgaa aaaagcggga cgtaacaacc
    28441 aaggtaaatt gactgtaaga caccatggtg gtggacacaa acgtcaatac cgtgttatcg
    28501 atttcaaacg taacaaagat ggtatcaatg caaaagttga ttctattcaa tatgatccaa
    28561 accgctcagc aaacatcgct ttagttgtat atgcagacgg tgaaaaacga tatatcattg
    28621 ctcctaaagg attagaagta ggtcaaatcg ttgaaagtgg tgctgaagct gacatcaaag
    28681 ttggtaacgc attaccatta caaaacattc cagttggtac agtagtacac aacatcgagc
    28741 ttaaacctgg taaaggtgga caaatcgctc gttcagctgg tgcaagtgct caagtacttg
    28801 gtaaagaagg taaatacgta ttaatcagat taagatctgg tgaagttcgt atgatcttat
    28861 ctacttgccg tgctacaatc ggtcaagttg gtaacctaca acacgaatta gttaacgttg
    28921 gtaaagccgg acgttcaaga tggaaaggta tccgtccaac agttcgtggt tctgtaatga
    28981 accctaacga tcacccacac ggtggtggtg aaggtcgtgc tcctatcggt agaccatctc
    29041 caatgtcacc atggggtaaa cctacgcttg gtaagaaaac tcgtcgtggt aaaaaatcat
    29101 cagacaaact tatcgttcgt ggacgtaaga aaaaataata taatcaactt atttgggtgt
    29161 gcggcttaaa gctgcacgca cataataaga agggaggcgc ccaaatggct cgtagtatta
    29221 aaaaaggacc tttcgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gaaggaagcg
    29281 aaaagaaaca agtaatcaaa acatggtcac gtcgttctac aattttccct aatttcatcg
    29341 gacatacttt tgcagtatac gacggacgta aacacgtacc tgtatatgta actgaagata
    29401 tggtaggtca taaattaggt gagtttgctc ctactcgtac attcaaagga cacgttgcag
    29461 acgacaagaa aacaagaaga taatatctat taagtagagg aggacatcct aatggaagca
    29521 aaagcggttg ctagaacaat aagaatcgca cctcgtaaag taagactagt tcttgactta
    29581 atcagaggta aaaatgctgc tgaagctatt gcaattttaa aattaacaaa caaagcttca
    29641 tcaccagtaa ttgaaaaagt attaatgtcc gctttagcta atgctgaaca taactatgac
    29701 atgaacacag atgaattagt agttaaagaa gcatatgcta acgaaggacc aacattaaaa
    29761 cgtttccgtc cacgtgcgca aggtcgtgca agtgcgatta acaaacgtac aagccacatt
    29821 acaatcgtcg taagtgacgg taaagaagaa gctaaagaag cttaattaac ttttaaggag
    29881 ggaatactgt gggtcaaaaa attaatccaa tcggacttcg tgttggtatt atccgtgatt
    29941 gggaagctaa atggtatgct gaaaaagact tcgcttcact tttacacgaa gatttaaaaa
    30001 tccgtaaatt tattgataat gaattaaaag aagcatcagt ttctcacgta gagattgaac
    30061 gtgctgcaaa ccgtatcaac attgcaattc atactggtaa acctggtatg gtaattggta
    30121 aaggcggttc agaaatcgaa aaattacgca acaaattaaa tgcgttaact gataaaaaag
    30181 tacacatcaa cgtaattgaa atcaaaaaag ttgatcttga cgctcgttta gtagctgaaa
    30241 acatcgcacg tcaattagaa aaccgtgctt cattccgtcg tgtacaaaaa caagcaatca
    30301 ctagagctat gaaacttggt gctaaaggta tcaaaactca agtatctggt cgtttaggcg
    30361 gagctgacat cgctcgtgct gaacaatatt cagaaggaac tgttccactt catacgttac
    30421 gtgctgacat cgattatgca cacgctgaag ctgacactac ttacggtaaa ttaggcgtta
    30481 aagtatggat ctatcgtgga gaagttcttc ctactaagaa cactagtgga ggaggaaaat
    30541 aataatgtta ctaccaaaac gtgtaaaata tcgtcgtcaa catcgtccta aaacaactgg
    30601 tcgttctaaa ggcggtaact acgtaacatt tggtgagttt ggtttacaag ctacaacaac
    30661 gtcttggatc acatctcgtc aaatcgaatc tgctcgtata gcaatgacac gttacatgaa
    30721 acgtggcggg aaagtttgga ttaaaatctt cccacataca ccatatacta aaaaaccttt
    30781 agaagtacgt atgggtgctg gtaaaggtgc ggttgaaggc tggatcgcag ttgttaaacc
    30841 aggtagaatt ttattcgaag ttgctggcgt ttctgaagaa gttgcgcgtg aagcactacg
    30901 tttagcaagt cacaaacttc cagtaaaaac taagtttgta aaacgtgagg aattgggtgg
    30961 tgaaacaaat gaaagctaag gaaattagag acttaaccac ttcagaaatc gaagaacaaa
    31021 tcaaatcttc aaaagaagag ctttttaacc tacgctttca gttagctaca ggtcaattag
    31081 aagaaactgc acgtattcgt acagtaagaa aaacgattgc acgtctaaaa actgttgctc
    31141 gtgaaagaga aattgaacaa agtaaggcta atcaataatt aagtaagagg aggttacaaa
    31201 agtgagcgaa agaaacgatc gtaaagttta tgtaggtaaa gttgtttcag acaaaatgga
    31261 caagactatt acagtacttg ttgaaactta caaaacacac aaattatacg gtaaacgagt
    31321 aaaatactct aaaaaataca aaactcatga tgaaaacaat tcagctaaat taggagacat
    31381 tgttaaaatt caagaaactc gtcctttatc agcaacaaaa cgttttcgtt tagtagagat
    31441 tgttgaagag tcagtaatta tttaatacaa gtttagagat aagggaggtt taactaatga
    31501 tccaacaaga aacacgcttg aaagtagcag acaactctgg tgctcgtgaa gttcttacaa
    31561 tcaaagtatt aggtggatct ggtcgtaaaa cagcaaacat cggcgatgtt atcgtatgta
    31621 ctgttaaaaa tgcaacacca ggtggcgttg ttaaaaaagg tgacgttgtc aaagctgtaa
    31681 tcgtacgtac taagtcaggt gttcgtcgta atgacggttc atacatcaaa tttgatgaaa
    31741 atgcatgtgt tatcatccgt gatgacaaag gcccacgtgg tactcgtatc ttcggacctg
    31801 ttgctcgtga attacgtgaa ggtaacttca tgaaaatcgt atcattagca ccagaagtac
    31861 tttaattaat aacaaccaat acaaataagg aggtgcccac atgcatatca aaaaaggtga
    31921 caacgttaaa gttatcgcag gtaaagacaa aggtaaagaa ggtaaagtaa ttgctactct
    31981 acctaaaaaa gaccgtgtcg ttgtggaagg tgttaacatt atgaaaaaac accaaaaacc
    32041 aactcaatta aatcctgaag gtggaatctt agaaacagag gcagcaatcc atgtttctaa
    32101 tgtacaatta ttggacccta aaacaaacga accaactcgt gtaggttaca aatttgttga
    32161 tggtaaaaaa gttcgtatcg ctaaaaaatc tggcgaagaa attaaatcta ataattaata
    32221 acaagttgaa aggaggatcc cactttgaac cgtttaaaag aaaagtttaa cactgaagtt
    32281 actgaaaact taatgaaaaa attcaattat agttcagtaa tggaagtacc aaaaatagat
    32341 aaaatcgttg tgaacatggg tgtaggtgac gcagtacaaa attctaaagt attagacaat
    32401 gctgttgaag aattagaatt gatcactggt caaaaaccat tagtaactaa agctaaaaaa
    32461 tcaatcgcga ctttccgttt acgtgaaggt atgccaatcg gtgcgaaagt aacacttcgc
    32521 ggtgaaagaa tgtatgaatt cttagacaaa ttaatttcag tatcattacc acgtgtacgt
    32581 gacttccaag gtgtttctaa aaaagcattt gacggacgcg gtaactacac tttaggtgtt
    32641 aaagaacaat taattttccc agaaatcgac tatgataaag taagtaaagt tagaggaatg
    32701 gatattgtta tcgtaacgac tgctaacact gatgaagaag ctcgtgaatt gttagctaac
    32761 ttcggtatgc cattccgtaa ataattattt aaaggaggct aattaagtgg ctaaaacttc
    32821 aatggttgct aagcaacaaa aaaaacaaaa atatgcagtt cgtgaataca ctcgttgtga
    32881 acgttgtggc cgtccacatt ctgtatatcg taaatttaaa ttatgccgta tttgtttccg
    32941 tgaattagct tacaaaggcc aaatccctgg cgttcgtaaa gctagctggt aataaaaaag
    33001 agtctgaaag gaggcaacaa tcaatgacaa tgacagatcc aatcgcagat atgcttactc
    33061 gtgtaagaaa cgcaaacatg gtgcgtcacg agaagttaga attacctgca tcaaatatta
    33121 aaaaagaaat tgctgaaatc ttaaagagtg aaggtttcat taaaaatgtt gaatacgtag
    33181 aagatgataa acaaggtgta cttcgtttat tcttaaaata tggtcaaaac gatgagcgtg
    33241 ttatcacagg attaaaacgt atttcaaaac caggtttacg tgtttatgca aaagctagcg
    33301 aaatgcctaa agtattaaat ggtttaggta ttgcattagt atcaacttct gaaggtgtaa
    33361 tcactgacaa agaagcaaga aaacgtaatg ttggtggaga aattatcgca tacgtttggt
    33421 aataaaaaat aaggaggtgc cataacatga gtcgtgttgg taagaaaatt attgacatcc
    33481 ctagtgacgt aacagtaact tttgatggaa atcatgtaac tgttaaaggt cctaaaggtg
    33541 aattatcaag aactttaaat gaaagaatga cattcaaaca agaagaaaac acaattgaag
    33601 ttgtaagacc atctgattct aaagaagata gaacaaacca tggtacaact cgtgctttat
    33661 taaacaatat ggtacaaggt gtttctcaag gatacgtaaa agtacttgaa cttgttggtg
    33721 taggttaccg tgctcaaatg caaggtaaag acttaatcct taacgttggt tattctcacc
    33781 cagtagaaat taaagctgaa gaaaacatta ctttctcagt tgagaaaaac acagtcgtta
    33841 aagttgaagg tatttcaaaa gaacaagttg gagcattagc atctaacatc cgttcagtaa
    33901 gacctccaga gccttacaaa ggtaaaggta ttcgttacca aggtgaatac gttcgccgta
    33961 aagaaggtaa aactggtaaa taatagataa actctaaaga aaggagtatt gaaatgatca
    34021 gtaaaattga taaaaataaa gtgcgtttaa aaagacatgc tcgtgttcgt actaacttat
    34081 caggtacagc tgaaaagcca cgtttaaacg tatatcgttc aaacaagcat atctacgctc
    34141 aaattattga tgataataaa ggcgtaacat tagctcaagc ttcttcaaaa gacagcgaca
    34201 ttgctactac agcaactaaa gttgaattag caactaaagt cggtgaagca attgctaaaa
    34261 aagctgctga caaaggcatt aaagaaatcg tatttgaccg tggaggatat ttatatcacg
    34321 gacgtgttaa agcattagct gaagcagcaa gagaaagcgg attagaattt taatttaaag
    34381 gagggacaaa tacatggctc gtagagaaga agagacgaaa gaatttgaag aacgcgttgt
    34441 tacaatcaac cgtgtagcaa aagttgtaaa aggtggtcgt cgtttccgtt tcactgcatt
    34501 agttgtagtt ggagacaaaa atggtcgtgt aggtttcggt actggtaaag ctcaagaggt
    34561 accagaagca atcaaaaaag ctgttgaagc agctaaaaaa gatttagtag ttgttccacg
    34621 tgttgaaggt acaactccac acacaattac tggccgttac ggttcaggaa gcgtatttat
    34681 gaaaccggct gcacctggta caggagttat cgctggtggt cctgttcgtg ccgtacttga
    34741 attagcaggt atcactgata tcttaagtaa atcattagga tcaaacacac caatcaacat
    34801 ggttcgtgct acaatcgatg gtttacaaaa ccttaaaaat gctgaagatg ttgcgaaatt
    34861 acgtggcaaa acagtagaag aattatacaa ttaaggaggg aaaactagtt atggctaaat
    34921 tacaaattac cctcactcgt agtgttattg gtcgtcctga aacacaacgt aaaactgttg
    34981 aagctttagg tcttaaaaag actaacagtt cagtagttgt tgaagataac cctgctattc
    35041 gtgggcaaat caacaaagtt aagcacttag taacagtaga agaaaaataa tcaaggaggt
    35101 gccgaaatga aattacatga gttaaaaccg gcagaaggtt cacgtaaaga acgcaatcgt
    35161 gttggacgtg gtgttgcgac aggtaacggt aaaacaagtg gtcgcggaca caaaggtcaa
    35221 aaagctcgtt caggcggtgg tgtaagacca ggatttgaag gtggtcaatt accattattc
    35281 cgtcgtttac caaaacgtgg ttttactaac ataaatcgta aagaatatgc tattgttaac
    35341 ttagaccaac ttaataaatt tgaagatggt actgaagtaa ctccagcttt attagtagaa
    35401 tctggtgttg ttaagaatga aaaatctggt atcaaaatac taggtaatgg ttcacttgat
    35461 aagaaattga cagtgaaagc tcataaattc tcagcttcag cagcagaagc tattgatgct
    35521 aaaggtggag cacacgaggt gatctaatga ttcaaaccct tgtgaacttc tttagaacaa
    35581 aagaggttcg taacaagatt ttcttcacac tagcaatgtt agtaattttt aaaataggga
    35641 cttatatacc agctccagga gtaaatcctg cagcttttga taatccccaa ggttctcaag
    35701 gtgccactga gttattaaat acttttggtg gcggagcctt gaaacgattt tctatttttg
    35761 caatgggtat tgtaccctac atcactgcat caatcgtaat gcaattatta caaatggata
    35821 ttgtccctaa attctcagaa tgggcaaaac aaggtgaagt aggtagaaga aagttaaata
    35881 acgttactcg ttatttagca atttctttag catttatcca atctataggt atggcattcc
    35941 aatttaataa ttatctcaaa ggtgcgctga ttatcaatca gtcaattatg agttatttat
    36001 taatagcact agttttgaca gcaggaactg ctttcttaat atggcttggt gatcaaatca
    36061 ctcagttcgg tgttggtaat ggtatttcta ttatcatatt tgcgggtatt ttatcaacgt
    36121 tgccagccag tctaattcaa tttggacaaa ctgcgttcgt tgggcaagaa gatacatcat
    36181 tagcatggtt gaaagtatta ggacttttag tgtcactgat actattaact gtaggtgcta
    36241 tttatgtact tgaagccgtt cgtaaaattc cgattcaata tgctaagaaa caaactgctc
    36301 aaagattagg ttcacaggca acttatctac ctttaaaagt gaactcagcc ggtgttattc
    36361 ctgtaatctt tgcaatggcg ttcttcttac tgcctagaac attaacgttg ttctatccag
    36421 ataaagaatg ggcacaaaac attgcgaatg ctgcaaatcc ttcaagtaat gttggtatgg
    36481 ttgtatatat tgtactaatt atactattca catacttcta tgcatttgta caagttaatc
    36541 cggaaaaaat ggctgataac cttaagaaac aaggtagcta tgttccaggt attagacctg
    36601 gtgaacaaac caaaaaatat attactaaag ttttatatcg tttaactttt gtaggttcta
    36661 tcttcttagc cgttatttca attctaccga tattagcaac taagtttatg ggattaccac
    36721 aatcaattca gattggtggt acaagtttac ttatcgttat tggtgtagcg attgaaacaa
    36781 tgaaatcttt agaagctcaa gtatctcaaa aagaatataa aggctttggt ggtagataat
    36841 tttaggaggg cacttatgaa tatcattttg atgggtttac ctggcgcagg taaaggaact
    36901 caagcaagtg aaattgtcaa gaaattccca ataccccaca tttcaactgg tgacatgttc
    36961 agaaaagcta taaaagaaga aactgaatta ggtaaagaag ctaagtctta tatggaccgt
    37021 ggcgaattag ttcctgatga agtgactgta ggtatcgtta aggaaagaat ttctgaagac
    37081 gatgcaaaaa aaggcttttt attagatggc ttcccaagaa caatcgagca agctgaggca
    37141 ttaaataata ttatgtctga gcttgacaga aacattgatg ctgtcatcaa tatcgaagtt
    37201 ccggaagaag aattaatgaa ccgtcttaca ggtcgtcgaa tctgtgagtc atgtggtaca
    37261 acgtatcatc ttgtatttaa tcctccgaag gtcgaaggta tttgtgatat cgatggtggt
    37321 aaattgtatc aacgagaaga tgataatcct gaaacggtag ctaatcgttt gagtgttaat
    37381 attaaacaat ctaagcctat tttagatttc tatgatcaaa aaggtgtatt gaaaaatatt
    37441 gatggttcaa aagatattag cgacgttacc aaagatgtca ttgatatttt agatcatttg
    37501 taatagatca acttttagcg gtctatttta agtgaggcat gatttaatca tattagatag
    37561 atgagagtgg caattaacga taattaattc ccgtaatttt tgtttaactt ataaaattaa
    37621 tcactgatag attcgtaaca ataaagttgt ctattgacga ttaccttact attgcaaaaa
    37681 gggggaagtt aatcaatggc taaacaagat gtaattgaat tagaaggtac tgtattagat
    37741 actttaccga acgcaatgtt taaagtagaa ttagaaaatg gtcatgagat tttagctcac
    37801 gtaagtggta aaatcagaat gaattacatt cgtattctac ctggcgacaa agtaactgtt
    37861 gagatgtctc cgtacgattt aacacgcgga agaattactt atcgttataa ataatcgtca
    37921 ctccataata tagggaggta taaaaatgaa agtaagacca tcagtaaaac ctatttgcga
    37981 aaaatgtaaa gtcattaaac gtaaaggtaa agtaatggta atttgtgaaa atccaaaaca
    38041 caaacaaaga caaggttaat aaaagagagg tgtaaattaa tatggcacgt attgcaggag
    38101 tagatattcc acgtgaaaaa cgcgtagtta tctcattaac ttatatatac ggtatcggta
    38161 cgtcaactgc tcaaaaaatt cttgaagaag ctaacgtatc agctgatact cgtgtgaaag
    38221 atttaactga tgacgaatta ggtcgcatcc gtgaagttgt agacggttat aaagtcgaag
    38281 gtgacttacg tcgtgaaact aacttaaata tcaaacgttt aatggaaatt tcatcatacc
    38341 gtggtatccg tcaccgtcgt ggtttaccag ttcgtggtca aaaaacgaaa aacaacgcgc
    38401 gtactcgtaa aggaccagtt aaaacggtag ctaacaagaa aaaataatag gtaaaggagg
    38461 caaattttaa atggcacgta aacaagtatc tcgtaaacgt agagtgaaaa agaatattga
    38521 aaatggtgta gcacacatcc gttcaacatt caacaacact attgtaacta tcactgatga
    38581 gttcggtaat gctttatcat ggtcatcagc tggtgcatta ggattcaaag gatctaaaaa
    38641 atcaacacca tttgcagcac aaatggcttc tgaaactgca tctaaatcag ctatggagca
    38701 tggtttaaaa acagttgaag taacagttaa aggacctggt ccaggtcgtg aatcagctat
    38761 tcgtgcatta caatctgcag gtttagaagt aactgcgatc agagacgtta ctccagtacc
    38821 tcataacggt tgtcgtccac caaaacgtcg tcgtgtataa tttatgatgg tattgttaca
    38881 ggtcactgag caaacatttt aaattaagtc gacgtatata aggaggatat ttaaatgata
    38941 gaaatcgaaa aacctagaat tgagacaatt gaaattagtg aagatgctaa attcggtaag
    39001 ttcgttgttg aaccactaga acgtggctac ggtactacac taggaaactc cttacgtcgt
    39061 atcctactat cttcattacc aggtgcagcc gttaagtata ttgaaattga gggagtttta
    39121 catgaattct cagcagtaga caatgtagtt gaagatgttt ctacaattat tatgaacatt
    39181 aaacaattag cattgaaaat ttactctgaa gaagataaaa ctttagaaat tgatgtacgt
    39241 gatgaaggcg aagtaacagc aagcgacatt acacatgata gtgatgttga aattttaaac
    39301 ccagagctta aaattgcaac agtatctaaa ggtggtcact taaaaattcg tctagttgct
    39361 aacaagggta gaggttacgc attagcagaa caaaataata ctagtgattt accaattggt
    39421 gtaatccctg ttgattcatt gtattcacct gttgaacgtg tgaactatac tgttgaaaat
    39481 acacgtgtag gtcaaagcag tgattttgat aaattaacat tggatgtttg gactaatggt
    39541 tcaatcacac cacaagaatc agtttcatta gcagcaaaaa taatgactga acacttgaat
    39601 atcttcgttg gtcttactga tgaagcgcaa aacgctgaaa tcatgattga aaaagaagaa
    39661 gatcaaaaag aaaaagtatt agaaatgtct attgaagaat tagacttatc tgtacgttca
    39721 tataactgct taaaacgcgc aggaatcaat tctgttcaag agttagctga caaatctgaa
    39781 gctgacatga tgaaagtgcg taatttaggt cgtaaatctt tagaagaagt taaatacaaa
    39841 ttagaagatt taggattagg attaagaaaa gaagattgat aaaggaggtt aactcatggg
    39901 ttacagaaaa ttaggtcgta cttctgatca acgtaaagct atgttacgtg acttagctac
    39961 atcacttatt attagtgaac gtattgaaac tacagaagct cgtgcaaaag aagttcgcag
    40021 tgttgttgag aaattaatca ctttaggtaa aaaaggagat ttagcttctc gtcgtaatgc
    40081 agctaaaact ttacgtaatg ttgaaatctt aaacgaagat gaaactacac aaactgcact
    40141 tcaaaaatta tttggtgaaa tcgcagagcg ttacacagaa cgtcaaggtg gttacactcg
    40201 tatccttaaa caaggccctc gtcgcggtga cggtgctgaa tcagtaatta tcgaattagt
    40261 ataagaattt atataaatac actaactact tttatataaa gcaaatgagt gcaacgataa
    40321 tgtttgccac atacaaatat tgtctagctc agagtacccc atcaactaaa taaagattta
    40381 aagcgtgaac tcaacaaact tatgatgggg tgcgcgcttt tttattgaat aagatatcgg
    40441 acttatgaac agaaatgttt ttaagtccga tttctttata tctacttttt tgagtgcgaa
    40501 tctaaaaata tgatcaagct agtttgatta atttcactag aactgtatga agtgaaagta
    40561 ttatcgttga atcaaacgcg gccaacccta tgttagtaaa tcatttattt tattaacgtt
    40621 tatacataat tgttagagag ggataaatgt ccttatcatt agttgttaaa caacacttta
    40681 aagactagaa atgagtcaga aaatactagt aatatataga gaacccagtc aatgtcatat
    40741 acagcgattt ataaatattg taaaatatga tagatacacg aatcgtaaag gagagagaca
    40801 agtggaggat aagaattcag ttattgtatt taaaaatgtt tcatttcaat atcaaagtga
    40861 tgcatccttc acattgaaag atgtttcttt tagtatacct aaaggtcagt ggacatctat
    40921 tgttggtcat aacggttctg gaaaatctac aattgccaag ttaatgattg gcatagagaa
    40981 agttaaatct ggagaaattt tttataataa tcaaactata acagatgata attttgaaaa
    41041 gttaagaaaa gacataggaa tagtattcca gaatccggat aatcaatttg ttggttcaat
    41101 tgtaaaatac gatgtggcat ttggactcga aaatcatgcg gttccacatg acgaaatgca
    41161 tagaagagtc ggcgaagcac ttaaacaagt tgatatgtta gaacgtgcag attatgaacc
    41221 taatgcatta tcggggggac agaagcagcg tgtggctata gcaagtgtat tagcacttaa
    41281 cccctctgtc attatattag atgaggcgac ttctatgtta gatcctgatg cacgtcaaaa
    41341 tttattggat ttagtgagaa aagttaaatc agaacataat attacaatca tttctattac
    41401 gcatgattta tctgaggcga tggaagcaga tcatgttatc gttatgaata aagggactgt
    41461 ctataaagaa ggcacagcga ttgaaatttt cgaccatgca gaagagttaa caacaatagg
    41521 tctagatttg ccattcccaa tcaaaataaa tcaaatgctg ggataccaaa catcattctt
    41581 aacttatgaa gggctggtgg atcaactatg actatacgct ttgacaatgt aagttatacc
    41641 tatcaaaaag ggacaccata ccagcatcaa gctattcatg atgttaatac agaatttgaa
    41701 caaggtaaat attacgccat cgttggacaa acgggtagtg gtaaatcaac attgatacaa
    41761 aatattaatg cactgttaaa gccaactact gggacagtta cagttgatga cattactatt
    41821 acacataaga ccaaagataa atatattaga cctgtaagaa aaagaattgg aatggtattt
    41881 caatttcccg aatctcaatt atttgaggac acagtagagc gtgaaatgat atttggacct
    41941 aaaaacttta aaatgaattt agatgaagcc aaaaactatg cccatcgtct gttgatggat
    42001 ttaggctttt caagagatgt aatgtcacaa tcaccatttc aaatgtcagg tggacaaatg
    42061 cgtaaaatag cgattgtatc gatattggca atgaatcctg atattatcgt ggttgatgaa
    42121 cccacagcag gacttgatcc acaaagtaaa cgacaagtaa tgcgattact aaagtcatta
    42181 caaacagatg aaaataaggc aattatccta atttcacatg atatgaatga agtcgcgcgt
    42241 tatgcagatg aagtcatcgt tatgaaagaa ggtagtatag tgtcgcaaac atcaccaaaa
    42301 gagctcttca aagataaaaa aaaattggcg gattggcata ttggtttgcc agaaattgtt
    42361 cagttacaat atgactttga acaaaaatat caaacaaaat taaaagatat tgccttaact
    42421 gaagaagcat ttgtaagctt gtataaggag tggcaacatg aaaaataaat tgattatagg
    42481 gcgatattta ccaattaatt cttttgttca tcatcttgat ccaagagcaa agttaatgtt
    42541 tgtttttctt tttattatat taatattttt ctgtcattca ccattaacat atctttgggt
    42601 gtttgcactt atcttattca ttatgagatt agcaaaaatt caattatggt tcctaatcaa
    42661 aggtttaaca ccaatatttt tctttttaat cttcacatta atgatgcata tatttttaac
    42721 taaaggtgga tatgtattag ttgaatggca tggcatcacg attgaaacta atggtatttt
    42781 agaagggctt tatatatcat tgcgtttaat tgggattgtg atgattgcaa caattatgac
    42841 actatctaca agtccaattg atttaacaga tgcgtttgaa agattacttg caccactaaa
    42901 gatgtttaaa ttaccagttc atcaattaag tatgataatg tccattgcgt tacgattcat
    42961 cccgacgtta atggatgagt tagataaaat tattttggca caaaagtcgc gtggttctga
    43021 aataagttca gggaacattg caacacgtat taaatcattt attccactac tagtgccact
    43081 attcatctca gctttccaac gcgccgaaga attagcggtc gcaatggaag ttaggggtta
    43141 tgatgccaat gttaagagaa cgagctacag acagcttaaa tggcaattga gagatacgtt
    43201 atctttaatc atgattatcc caattgctat tattttattc gtattaaaat attcaggagt
    43261 gtaaattcat gcgtatatta gtagaaattg cgtatcaagg aaataatttt ctaggttttc
    43321 aaattcaaca aaatggtcga acggtacagc aacaatttga aaagctatta caacgcatgc
    43381 ataaaagaca tgtgagaatc catccttcaa gtaggactga tagaggtgta catgcgatac
    43441 agcaatactt tcatttcgac acagagctga atatacctat gtcacaatgg caatatgcaa
    43501 tgaaccgaac gttacctgat gatatttatg tcaacaatgt agttacagtc gatgacgatt
    43561 ttcattgtcg ttacgattgt gtaggtaaac gctatcgata taaagtatat caagcacaac
    43621 accgcgatcc atttcaaagt ggtttgaaaa catttattcc tgaaacgttg gatttagata
    43681 aaatgaacag agcggcacaa caattcatag gtacacatga ttttacggga ttttgttcgc
    43741 aaaaaactga agtagaaagc aaggttagaa cattatacca aagtgaaatt gtaaagacag
    43801 atgatggctt tgattatatt gtcactggat ctggattttt atataacatg gtgcgtgtct
    43861 tagttgcatt tttaatagaa gttggaaaag gacgacatga aatttctgat gtgccgaagc
    43921 tacttgaatc aaagaatcgt aagaatgttc ctttcactgc aacggctgaa ggattatatt
    43981 tagagaagat ttatttagat gaaaatgagt tattaaaaga ctttggcaac gatatcaaaa
    44041 tacatcgtaa aaaatcatta caaaatgact gattgccatt gacaaaaagc ttgtgaaatt
    44101 ataagattat taacggtatt gttttatatc caccccacga taagccccgg aaacttattg
    44161 tgttacaaga tatataagca gaaacgaaca acagttaaca aaataaatga aattaaacgt
    44221 tttaaaaatg aaacaaatga aatcatctat taggttatga aactgtttat agcttgaata
    44281 gaagcattta ttttttagga ggacaattat tatgcgtcaa acatttatgg caaatgaatc
    44341 aaacattgag cgcaaatggt atgttatcga tgctgaaggc caaacattag gtcgtttatc
    44401 atcagaagta gcatctatct tacgcggtaa aaataaagta acttacacac cacacgttga
    44461 tactggtgat tatgtaatcg ttattaatgc atcaaaaatc gaatttactg gtaacaaaga
    44521 aactgacaaa gtttactacc gtcactcaaa tcacccaggt ggtatcaaat caatcactgc
    44581 tggtgaatta agaagaacta acccagaacg tttaattgaa aactcaatta aaggtatgtt
    44641 accaagcact cgtttaggcg aaaaacaagg taaaaaatta tttgtatatg gtggcgctga
    44701 acatccacac gctgcacaac aaccagaaaa ctacgaatta cgtggttaat tagaaggagg
    44761 aaatgacttt ggcacaagtt gaatatagag gcacaggccg tcgtaaaaac tcagtagcac
    44821 gtgtacgttt agtaccaggt gaaggtaaca tcacagttaa taaccgtgac gtacgcgaat
    44881 acttaccatt cgaatcatta attttagact taaaccaacc atttgatgta actgaaacta
    44941 aaggtaacta tgatgtttta gttaacgttc atggtggtgg tttcactgga caagctcaag
    45001 ctatccgtca cggaatcgct cgtgcattat tagaagcaga tcctgaatac agaggttctt
    45061 taaaacgcgc tggattactt actcgtgacc cacgtatgaa agaacgtaaa aaaccaggtc
    45121 ttaaagcagc tcgtcgttca cctcaattct caaaacgtta attgtcggac gatatataca
    45181 aaacacctcg aagttatgtc gaggtgtttt tttacgtttg tgtggggaat atggaacgtg
    45241 tagagtataa tgaattttcc tttatttaat gtctcaggaa tagaataaat tcaactatgt
    45301 attagtgatt tgcacgtatg atttgtacat gcttaaacaa tcaaaagtgt ccattattaa
    45361 attatcattt agcttgcgga ggccgtatga taaccggacg attgtgttct aatcatagag
    45421 gctcaatgga agaactacgg gacgaaaacc agacgataac tcaagaacca tgatttaaga
    45481 gtattatcaa aataaggagt gacttaattg aaagatttta tattttggaa gaaagctttt
    45541 attcctgttt attttattgt tgcgatgctg gtgtttctac tttttaggtt ttatattaaa
    45601 acagacaact tttctatgta tttaatgatt atcttcttaa tttgtttagg aactgcatct
    45661 atcatttata actataaaaa caatcgataa atataagtat gaaatgtata agtcacagtt
    45721 agatctaagt cttgctgtgg ctttttttat cgaaatgaca attttaaatc gccaaaaaca
    45781 acattttcag tccgccataa aacaacaatt tcaaatcgcc attgactaaa cacgacatcc
    45841 aaaaattatt gtaacaattc ttcacatcaa tcaatcctcc atcactcata atcactccct
    45901 acaaaatcta ctcatgcatt tttggaatac ttagtattac aaataacgat ttttattcat
    45961 cttacaaagg ttatataatg taatgaaggc aatttttatg tattgcaaat ctaatcgtac
    46021 atgtaaaatt ttgataaaca tcattaattt tgcgtaacta tcattagatt acaaatcaca
    46081 atgtaattac atgtaataca catctataca tcacatttga agggaaatga atataaatga
    46141 ctgataaaaa gtacactgca gccgatatgg ttattgatac tttgaaaaat aatggggtag
    46201 aatatgtttt tggtattccg ggtgcaaaga ttgactatct atttaatgct ttaattgatg
    46261 atggtcctga acttattgtc acgcgtcatg aacaaaacgc cgcaatgatg gcgcaaggta
    46321 ttggaagatt aacaggcaaa ccgggtgtag tacttgttac aagtgggcct ggtgtaagta
    46381 atttaacgac tggactatta acagctacat ctgaagggga tcctgtctta gcgttaggcg
    46441 gccaagtgaa acgtaatgat ttattacgat taacgcatca aagtattgat aatgctgctt
    46501 tattaaaata ttcatcaaaa tatagtgaag aagtacaaga tcctgaatca ttatcagaag
    46561 ttatgacaaa tgcaattcga attgctactt cagggaaaaa tggcgcaagt tttattagta
    46621 ttccgcaaga tgtgatttct tcaccagttg aatctaaggc gatatcactt tgtcaaaaac
    46681 caaatttagg tgtaccaagt gaacaggata ttaatgatgt cattgaagcg attaaaaatg
    46741 catcatttcc ggttttacta gctggaatga gaagttcaag tgcagacgaa acgaatgcca
    46801 ttcgcaaatt agtagagcgt accaatttac ccgttgtaga aacattccaa ggtgcgggtg
    46861 tgattagtcg tgaattagaa aatcatttct ttggccgagt gggtttattc cgtaatcaag
    46921 ttggtgatga attattacgt aaaagtgatt tagttgttac aatcggttat gatcctatag
    46981 aatatgaggc tagtaactgg aataaagaat tagatacgca aataatcaat attgacgaag
    47041 ttcaagctga aattactaat tatatgcaac cgaaaaaaga gttgattggt aatattgcta
    47101 aaacgattga aatgatttct gacaaagtag atgaaccatt tataaatcaa caacatttgg
    47161 atgaattaga acagttaaga gcgcatattg atgaagaaac tggtattaaa gcgacgcatg
    47221 aagaaggcat cttacaccca gttgaaatta tagaatctat gcaaaaggta ttaactgatg
    47281 aaacaacagt gacagttgat gttggtagtc actatatttg gatggcacgt aatttcagaa
    47341 gttacaatcc aagacattta ttatttagta acggtatgca aacgcttggt gtagcattac
    47401 cgtgggcaat ttcagctgca cttgtacgcc ctaatacgca agttgtatcc gttgctggcg
    47461 atggaggctt tttattttca tcacaagatt tagaaacggc cgtacgtaaa aatctaaaca
    47521 ttatccaact tatttggaat gatggaaaat ataatatggt tgaattccaa gaagaaatga
    47581 aatataaacg ttcatcaggc gtagacttcg gtcctgtaga tttcgtaaaa tatgctgaat
    47641 catttggcgc aaaaggttta cgagttacta atcaagaaga attagaagcg gcaattaaag
    47701 aggggtatga aacagatggc ccagtattaa ttgatatacc tgtaaactac aaagataata
    47761 tcaaactttc aacaaatatg ttacctgacg tatttaacta aaacaaagat aaatgttaat
    47821 gaggagtggg aaattttatg gctagtgttt tatatcaaca tggtacatta ggcacattaa
    47881 tggcaggatt attaaaagga actgcatcaa taaatgaatt attgcaacat ggtgacctag
    47941 gtatcgctac actaacaggt tcaaatggtg aggtcatctt tttagatgga aaagcttacc
    48001 atgcaaatga acataaagaa tttgtagaat taaaaggtga tgagttaaca ccatatgcaa
    48061 ctgtaactaa attcgtagca gatacaagct atgaaacgaa agataaatct tctgaagcag
    48121 tttttgcaga aattaaggaa aagatgttga gtgaaaattt attttctgca gtaaaaattt
    48181 caggcttatt taaaaaaatg catgtacgta tgatgccggc acaagagcca ccttatacac
    48241 gtttaatcga ttcagctaga agacaacctg agcaaactga aacatacgtc aaaggttcag
    48301 tcgttgggtt ctttacacca gaactattcc atggtatcgg atcagcagga ttccatgtac
    48361 actttgcaaa tgatgatcgt aattttggtg gacatgtctt agattttgaa gtagaagatg
    48421 ttaaggtaga aatccaaaat atagaaacat ttgaacagca ttttccaatt caagatgaag
    48481 atttcataaa tgcaaatatt gactataaag atattgcgga cgaaattaga gaagctgaat
    48541 aatgaatgcg gaaatgtaat gacggtttat gaaaattgac ttcaaaatgc gttatttgga
    48601 aatggtagtt tttttatatg attaagcaag actacaatag aacaaatata tttataatta
    48661 cgtctaagta ataaaataaa tcccttcact attagcggta gtgaggggat ttatttaggt
    48721 tccagatatt tgagatttgc tgttatgttt agataataaa ttgaggaaga cattaatata
    48781 gaatttactt tagtataggc caatctaatc tattgtcagt agaagcaatc aataataaat
    48841 tacaagttcg atcattcatt tcatactaaa aagacaaacg caatttttca gcgtttgcct
    48901 catgtaatta ttcttcattt tcaaattatt atttaactac aatttgtatc tgtttgattg
    48961 tacttggatc aattgaattt ttagcgtcgt catttttctt tagattcact acttttttat
    49021 taccatcatt caaagtaagt gtataagttg cagtttgcgc attattaatt ttttctgttg
    49081 taacaccacg ttgtgaagct aattcatttt ttactttact gtcaatttct tgataaagta
    49141 cctttttatt ttctggaaaa acaaagtaag ttcgatgtaa tgcagtaata ccatctactg
    49201 agatcgtgta aggtacagtg tgtttcccat ccattgtcaa ttccttataa ccgttattac
    49261 tatctgcaga tgcttcatga ctcggtaaag ctgcacctac tgttgataat aatcccaatg
    49321 ctaaagtggc agtaacaaat gattttagtt tcataactca atctcctttt tgttttgtag
    49381 gatattacac tatttgtata acacgtttca taaagtttaa aacatacttt ttgatttttt
    49441 tatatttttg atttttagta atagttttta acgagggata tgactcgtgt attttgaaaa
    49501 aagaaatata ataaaccatc atgttactat aattttaaat cattaattat catctttatt
    49561 taatgtttgt tgtaattcaa agcgcacgcc ggattcatta gaagtcgacg tatttttgtt
    49621 tgtaatagag tatccagtca ttgaaatttt agattcaata tctgaagcgg tatttgtaga
    49681 ttcgggatta tagaagctac attcataagt tttatcatct ttctttttaa gtataaacat
    49741 accttttgct ttaacttcga ctttagtgtt gttaatgata aaagtttgag tgctatcgct
    49801 gtaattaaca ccagcccaaa ccgattcatt atctttcaca acgtggaagt catcatcttg
    49861 tttaacgaca gttactttat ctttttttgt attaaagtca tctttagata agcctggata
    49921 caacacatat gcatatttac tatctgtatt agaatgcttt tgagttactt cataatattg
    49981 attagtttta ctgtcttgct tttgactttt atttatatca ctccacttac cagtatgact
    50041 ttcttttttt acagttattt tcgattcgtt taaaaaatga tagcctatat tgtttttagg
    50101 tttattggtt gactctaaaa agacggaatt ggttccctga ttatcggaag cggttgtttg
    50161 tttatcgtct gtatataacg tatacccatt cgctttgcga ttttcaatcg ttgtaactgg
    50221 attctttgat gaatcagtac ttttaatgcc agttcctaag aagacaattt tatcgtttaa
    50281 tatgaaatat gattttttgg cagttaaagt tttgtcctga ttttcaaaat ccattccgat
    50341 actagcatgt tggtcatcga attttgttcc gcctacaaaa gttttactcg actttttaac
    50401 atccgtacct ttaggttctt cattttctaa agttgttgta cctgctaaac gtttcatatc
    50461 agctgtcgcc cagaagttat cacggtagtg tttcacatcg ctattgtata aataagacat
    50521 tccagcacca gtgtgccaac cttttaagtt ctctccgttg atactttcgt aacgtgcgac
    50581 gtttttcgac gtcatactta aaccaaatgc aaagtctaag tctttattgt gataggtgac
    50641 acgatccatg tcattatata ttttaagttg ttgtgttaaa ccgttagtag aaatagtgct
    50701 gtcttccatt aaagacttca ttttgcttat atccgaataa gagcttaatg tatcattttg
    50761 gccgtaactt gaatcagatt taacagaagt tttaacgatt tgcttatact tagcttttgt
    50821 tgaatcatcc atggcatcac tcaatctcaa caatgatttc attacagtcg ctgacgctgt
    50881 atgactcgtt tcattttcac gactgatagc tctacctctt gataaatcca tcatttcacc
    50941 tttataaatg agtggcataa atccgtcgtc aatccatgac tttaaggttg tatcattttg
    51001 ggttttatca ttaaaaggtg tttcttttat catcggcatc atttgagaaa tcccctctaa
    51061 gagtacaacg ccataagcac cagtgtatgg gacgtcttga tgatcaatat aagagccgtc
    51121 tttatagaat ccattacgtt ctttaccagt tgcattactt tgaacgtaag tgaagacttt
    51181 attaaatgca tctattgaat ttttagttat atctttatct tcttcgataa tactttctaa
    51241 aagttttact ttagcaatgt ctactaaatt tcccccttta gcttgttcag gttgtcctac
    51301 tgatgataat attttgtcac tttctggggc gaaagttttg ataggggcag tgtatttttt
    51361 cttttcgtca tttgaaaatt gatcattcag caatataagg gtatttgtta atgctctagg
    51421 tgttccaatt tcataatccc accaatttaa attggtattt ctagaagttt tatttttaaa
    51481 gtttgaggtc aaatcagcaa cttttttatc tggttctttt ccatatgcat ttttatgcag
    51541 ccactcaagg gcatctttca ctttcttctt gttttcatct atttttaact tagtatcttt
    51601 atgcttcatc gcttctgcga ttttctcaat attacgatag gtacgagtca tatccgcaga
    51661 cttcgtatct aaatcttttg agttttccca caagtatttc ctatcacttt cagttttcat
    51721 ctcactaagt aatttctttg cctcattctc cgtagcctca aacttcttct tcattgcgtc
    51781 attcttctca tcatacttat cataaccata gttaacgtcc agccatgtgt tcctcaactt
    51841 ttcataatct ggcgtttgaa cattcgtatc agccacagcg atttgatatt tatcaatact
    51901 tctgaattca ccaccattca aagtaatcac accagccatt aataacgtaa tggtggataa
    51961 tttttgccat ttctttattc tatatgtcat tgacatgtct cctttttgtg ttgcgcgtgc
    52021 gcaatgaata ttatgattaa ataatgattc aatttttcaa aattcgttaa cgtatacaaa
    52081 tgactgtcta ctgtcaaaca atccacaaag aatgttgatg catataaaca atcgatcacc
    52141 caaattttcc gcatcaccca aaaattgcaa cgaaaataat tt
//
LOCUS       DAMZLE010000005        51047 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMZLE010000005 DAMZLE010000000
VERSION     DAMZLE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 51047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.005"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            116..1594
                     /gene="mqo"
                     /locus_tag="POB92_000285"
     CDS             116..1594
                     /gene="mqo"
                     /locus_tag="POB92_000285"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6577811.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(1660..2115)
                     /locus_tag="POB92_000286"
     CDS             complement(1660..2115)
                     /locus_tag="POB92_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDI6577812.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVNTINKQLKQLK"
     gene            complement(2112..2555)
                     /locus_tag="POB92_000287"
     CDS             complement(2112..2555)
                     /locus_tag="POB92_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6577813.1"
                     /translation="MMKLNLFINAKETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(2718..4091)
                     /gene="hssS"
                     /locus_tag="POB92_000288"
     CDS             complement(2718..4091)
                     /gene="hssS"
                     /locus_tag="POB92_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDI6577814.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDNTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            complement(4084..4758)
                     /gene="hssR"
                     /locus_tag="POB92_000289"
     CDS             complement(4084..4758)
                     /gene="hssR"
                     /locus_tag="POB92_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDI6577815.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            4894..5949
                     /locus_tag="POB92_000290"
     CDS             4894..5949
                     /locus_tag="POB92_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577816.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            5949..6614
                     /locus_tag="POB92_000291"
     CDS             5949..6614
                     /locus_tag="POB92_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577817.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(6845..7813)
                     /locus_tag="POB92_000292"
     CDS             complement(6845..7813)
                     /locus_tag="POB92_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI6577818.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            8008..8463
                     /locus_tag="POB92_000293"
     CDS             8008..8463
                     /locus_tag="POB92_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6577819.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            8703..10085
                     /locus_tag="POB92_000294"
     CDS             8703..10085
                     /locus_tag="POB92_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577820.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKTVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            10116..10244
                     /locus_tag="POB92_000295"
     CDS             10116..10244
                     /locus_tag="POB92_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577821.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            10354..11562
                     /locus_tag="POB92_000296"
     CDS             10354..11562
                     /locus_tag="POB92_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6577822.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(11792..12343)
                     /locus_tag="POB92_000297"
     CDS             complement(11792..12343)
                     /locus_tag="POB92_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6577823.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            12464..13111
                     /locus_tag="POB92_000298"
     CDS             12464..13111
                     /locus_tag="POB92_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6577824.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            13124..15055
                     /locus_tag="POB92_000299"
     CDS             13124..15055
                     /locus_tag="POB92_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDI6577825.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(15301..15939)
                     /locus_tag="POB92_000300"
     CDS             complement(15301..15939)
                     /locus_tag="POB92_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577826.1"
                     /translation="MTNPNQRLEPFDETFQQSNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            16147..17067
                     /locus_tag="POB92_000301"
     CDS             16147..17067
                     /locus_tag="POB92_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6577827.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGPQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDDDVTQNN"
     gene            complement(17175..17594)
                     /locus_tag="POB92_000302"
     CDS             complement(17175..17594)
                     /locus_tag="POB92_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577828.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(17591..18082)
                     /locus_tag="POB92_000303"
     CDS             complement(17591..18082)
                     /locus_tag="POB92_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6577829.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLLELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            18179..19126
                     /gene="corA"
                     /locus_tag="POB92_000304"
     CDS             18179..19126
                     /gene="corA"
                     /locus_tag="POB92_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDI6577830.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            19157..20206
                     /locus_tag="POB92_000305"
     CDS             19157..20206
                     /locus_tag="POB92_000305"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI6577831.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMYSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(20254..21462)
                     /gene="gltS"
                     /locus_tag="POB92_000306"
     CDS             complement(20254..21462)
                     /gene="gltS"
                     /locus_tag="POB92_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDI6577832.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKLLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTSQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(21799..22407)
                     /locus_tag="POB92_000307"
     CDS             complement(21799..22407)
                     /locus_tag="POB92_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDI6577833.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            22806..23426
                     /locus_tag="POB92_000308"
     CDS             22806..23426
                     /locus_tag="POB92_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6577834.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPKTPEETDNQL"
     gene            23657..24556
                     /locus_tag="POB92_000309"
     CDS             23657..24556
                     /locus_tag="POB92_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577835.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            24549..25781
                     /locus_tag="POB92_000310"
     CDS             24549..25781
                     /locus_tag="POB92_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577836.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            25765..25950
                     /locus_tag="POB92_000311"
     CDS             25765..25950
                     /locus_tag="POB92_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577837.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            25999..26325
                     /locus_tag="POB92_000312"
     CDS             25999..26325
                     /locus_tag="POB92_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDI6577838.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            26355..27374
                     /locus_tag="POB92_000313"
     CDS             26355..27374
                     /locus_tag="POB92_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDI6577839.1"
                     /translation="MIVEVEHQRQSVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(27610..28263)
                     /locus_tag="POB92_000314"
     CDS             complement(27610..28263)
                     /locus_tag="POB92_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDI6577840.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPIHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNELYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            28437..29123
                     /locus_tag="POB92_000315"
     CDS             28437..29123
                     /locus_tag="POB92_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDI6577841.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(29189..30448)
                     /gene="sdpC"
                     /locus_tag="POB92_000316"
     CDS             complement(29189..30448)
                     /gene="sdpC"
                     /locus_tag="POB92_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDI6577842.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            30738..31673
                     /locus_tag="POB92_000317"
     CDS             30738..31673
                     /locus_tag="POB92_000317"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDI6577843.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQESTSGTSFRQILEEDDNTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLNH
                     VSPPIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(32166..33050)
                     /locus_tag="POB92_000318"
     CDS             complement(32166..33050)
                     /locus_tag="POB92_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6577844.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIKSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            33210..34871
                     /gene="hutU"
                     /locus_tag="POB92_000319"
     CDS             33210..34871
                     /gene="hutU"
                     /locus_tag="POB92_000319"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDI6577845.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            34871..36109
                     /locus_tag="POB92_000320"
     CDS             34871..36109
                     /locus_tag="POB92_000320"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDI6577846.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
     gene            36385..37506
                     /locus_tag="POB92_000321"
     CDS             36385..37506
                     /locus_tag="POB92_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDI6577847.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            37617..38498
                     /locus_tag="POB92_000322"
     CDS             37617..38498
                     /locus_tag="POB92_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6577848.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYHPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            38985..39554
                     /locus_tag="POB92_000323"
     CDS             38985..39554
                     /locus_tag="POB92_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577849.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      39607..39702
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            39779..41092
                     /locus_tag="POB92_000324"
     CDS             39779..41092
                     /locus_tag="POB92_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDI6577850.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKSYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            41259..41765
                     /locus_tag="POB92_000325"
     CDS             41259..41765
                     /locus_tag="POB92_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDI6577851.1"
                     /translation="MAKFNVENEHVEVEVEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(41823..42587)
                     /locus_tag="POB92_000326"
     CDS             complement(41823..42587)
                     /locus_tag="POB92_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6577852.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            42917..44521
                     /locus_tag="POB92_000327"
     CDS             42917..44521
                     /locus_tag="POB92_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6577853.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(44587..45117)
                     /locus_tag="POB92_000328"
     CDS             complement(44587..45117)
                     /locus_tag="POB92_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577854.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            45385..46302
                     /locus_tag="POB92_000329"
     CDS             45385..46302
                     /locus_tag="POB92_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDI6577855.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFISPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            46377..47012
                     /locus_tag="POB92_000330"
     CDS             46377..47012
                     /locus_tag="POB92_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6577856.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            47023..47214
                     /locus_tag="POB92_000331"
     CDS             47023..47214
                     /locus_tag="POB92_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577857.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(47310..47495)
                     /locus_tag="POB92_000332"
     CDS             complement(47310..47495)
                     /locus_tag="POB92_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577858.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIIVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(47492..47848)
                     /locus_tag="POB92_000333"
     CDS             complement(47492..47848)
                     /locus_tag="POB92_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577859.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            48128..49507
                     /locus_tag="POB92_000334"
     CDS             48128..49507
                     /locus_tag="POB92_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6577860.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     IAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(49894..50766)
                     /locus_tag="POB92_000335"
     CDS             complement(49894..50766)
                     /locus_tag="POB92_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6577861.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT        17453 a         7116 c         9545 g        16933 t
ORIGIN      
        1 aatagagcat caccatggga acctattgag acacttattg atttaaagtg gtattaatat
       61 gtcgtatttc tcgaacgttc cattattcat tttaaaaagg gggactgtat ttgttatgac
      121 aacacaacat agcaaaacag atgtcatctt aattggtggc ggtattatga gtgcaacatt
      181 aggaacatta cttaaagaat tatcacctga gaaaaatatt aaagtgtttg aaaaattagc
      241 acaacctggc gaagagagtt caaatgtatg gaataatgcc ggtacagggc attcagcact
      301 ttgcgagttg aactatacaa aagaaggtaa ggatggcaca gttgattgta gtaaagcaat
      361 taagataaat gagcagtacc aaatttcaaa acagttttgg gcatatttag ttaaaacagg
      421 acaattagat aacccagatc gctttattca agcggtgcca cacatgagtt ttgtcattgg
      481 cgaagataat gtagctttta taaaaagtcg tgttgcaacg ttaaagaaaa gtgttttatt
      541 cgaaaaaatg aaattatcgc aagatgaaga agaaatgaaa tcttgggtac cgttaatgat
      601 tgaaggtcgt aagtctgatg aaccaattgc tttaacttat gatgaaactg gtacagatgt
      661 taactttggt gcgttaactg caaagttatt tgaaaattta gagcaacgtg gtgtgggaat
      721 tcaatataag cagaatgtat tagacatcaa gaaacagaaa tctggggcat ggctagttaa
      781 agttaaagat ttagaaacta atgaaacgac aacatatgaa tctgattttg tatttattgg
      841 tgctggcggt gcgagtttac cattactcca aaagactggg attaaacaat caaaacatat
      901 tggtggtttc ccggtaagtg gattattcct gcgctgtaca aatcaagaag tgattgatcg
      961 tcatcatgct aaagtgtacg gaaaagcagc agtgggtgcg ccaccaatgt cagtgccgca
     1021 cttagataca cgttttgtag acggcaagcg ttcattgtta tttggtccat ttgcaggttt
     1081 ctcacctaaa tttttaaaaa caggttcaca tatggattta attaaatcgg ttaaaccaaa
     1141 taatatcgtg acgatgttat ctgcaggtat caaagaaatg agtcttacga agtatttagt
     1201 gtcacaattg atgttatcta atgatgagcg tatggatgat ttaagagtct ttttcccaaa
     1261 tgctaaaaat gaagattggg aagtgattac agcagggcaa cgtgtccaag taatcaagga
     1321 tactgaggat tctaaaggta acttacaatt tggtactgaa gtcattacgt cagatgatgg
     1381 cacattagct gcattacttg gtgcatcacc tggtgcgtca acagctgtag atattatgtt
     1441 tgatgtttta cagcgatgct atcgtgatga attcaaagga tgggaaccaa agattaaaga
     1501 aatggtgccg tcatttggtt atcgcttaac agatcatgag gatttatatc acaaaattaa
     1561 tgaagaagta actaagtatt tacaagttaa ataataaatg aaacggtaat gtctttttta
     1621 atgtgataga cattaccgtt ttttagttgt taatgaaaat cattttaatt gtttcagttg
     1681 cttgttaata gtgtttacgt agttcttatt tttaaagaat tgaattatcc aaattaatac
     1741 ataaaccaca atgaagataa ttgtgaatat gattagataa tgcactgtta gtggaaacca
     1801 accggcaagc attgctaaag gcaagaatcc gacatacgtt gttatgaaat gcattatagt
     1861 tgctttagta atgctccaat ctgtgtattt aaagataaaa tctccaagga aaaagacgac
     1921 gcctatgagt aaccataaaa tgatagaaat caacattacg gtagtttctg tgaaatgcgt
     1981 ataatacaat atgccaatag ttgattgtgg gttcagtgga taatatttgc cgtctgcaaa
     2041 taacatacta aagaacagtg aaagggacaa accaatgatt aagctaataa ataatgagtt
     2101 tttcaaattt ttcatattga taagcgctcc tttatagatt ttaaataacg tctagaagaa
     2161 taggtgtagt gtgcatcttt aagatacata cgtataagtc catttggctc taataataat
     2221 ttttcaatgt aatacttgtt gacgatttct gatttggaaa tgcgaatgaa atgttgtggt
     2281 aactgttttt ctagttcata aagtcgtaat tttagtttga atttttgatt gcttgtaatg
     2341 gctgttacat ttttattaat aacttggaat gttataacat ctgatacatt aatgatatgg
     2401 atttctttgt ctatgtatcc gactaatgta tgtgatttgt ctaaatcatt gactgcatta
     2461 ataatacttt gaacgtgatc attcatttta ggtgcatgta tatcaatata agattccgtc
     2521 tcttttgcat tgataaataa attgagtttc atcataggtt aatgcctcct tcaaaattat
     2581 taaaccataa atgaccatcg atatatttaa attttgttga atggtagaaa ttaaatgtta
     2641 agtggctaga aagcgctaat caatataaaa gatacctcct gaaataaaaa cagaaatgtt
     2701 ttttcaggag gtagagatta aagtgaatta tttggcagtg taatagtaaa ggtggttaca
     2761 tactcgttac tttgtgtgaa ttggattgta ccatgatgca attcaatgat ggattttgta
     2821 attgcaagac ctaaaccatt gctattatca tgtttgctca ctttataaaa acgttcaaat
     2881 aaacgtgctt cagcttgtgg actaattggt gaaccatcat tacttattgt aaaaatgatg
     2941 ttgttgtgac tatgttgcaa agcgatgtca atggcaccac caacatctgt atacttaata
     3001 gcatttatta ataaattact caatgcttga tgtaacaaac gttgatttcc taggaaattg
     3061 attgattcta gatcagctaa aataattaac gacttttcat cagcagcaaa ttgttcatgt
     3121 cgaatgatat ctttaatgag ttgatcgact tgaatcttat catcgaataa taaatgttgg
     3181 tgattatcta attcggataa gagcaacaat tcagttgtta atccgctcag ctgcgttgta
     3241 atggtataaa tatcatttat atattgttgt cttaatgtat tatcagaagt ttgttgtaac
     3301 tcgcttaata aatgatgaat atgtgttaat ggtgttttga tttcatgaga tacattttgt
     3361 acaaaatgct gcctcatttg atcaacttgt cccaatgact ctctcatctt attaaagtga
     3421 tactgtaatg ttccaatttc atctttgcgt gtttgtttga taggtgtttc aaaatcacca
     3481 tcaattaagc gttcggtcgc tagctttaac ttttttactg gacgaatgat tgaataggtt
     3541 gatgcgataa ctaatgaaat cgagataaat aataacaaca ttaacaagac ggctaagaat
     3601 gttctaaact cgctaaatgt ttctccaata tctggacgca tgaatactgc tattgaaccg
     3661 tcttttgtct taaagttaat accaacagta ttatctgtta cattgtcgaa aaatccagtt
     3721 acgaataatg cgaatggttt atctttaata ccatggtaat ctttattatt taatacgttg
     3781 ttaatagcat tttgtgataa agtatcttct ctaaatggct ccccataaaa tgtcttatga
     3841 cctttttgat caacggtcat tatttggtaa ttcatttgcc ctaaatgttt gaaatattgt
     3901 tgaatgtgcg ttggtttagc tgattgttca tattgtcttg cttctttaag tgtcttcatg
     3961 attttcgcgt cattagatgc ttttaaatta taatgatagt aaacatttgt taatacaaaa
     4021 cttattaatg cactaaataa aataaccgta atggaataaa tcgcaattct agcatagagt
     4081 gttttaaaca tgattctcca ccttatagcc ttgtcctctt actgtttcaa ttgtaagtgt
     4141 ggcatttaat ttttttaatc tttggcgtag tcgcttaata tgaacgtcaa ctgttcgttc
     4201 atctccttca taatcatagc cccaaatttt ttctattatc tgttcacgtg taaagatttg
     4261 cttaggacgt gctgcaagca taaataataa ttgaaattcc ttgttcggta acgtcatcgt
     4321 tttattagat acttggagtt ccaaatagga ttggtttagc gttaagttgc caatagtcat
     4381 ttctgaattt gaattgatat tatatcgacg taataccgca cgaattctaa aaataagttc
     4441 cttaacctca aagggtttgg ttacataatc gtcagtaccg cttataaacg cacgctcttt
     4501 gtcactaagt gcatcccgcg ctgttaacat aataactggt atatcataat cattttttaa
     4561 tgtattacat aattgaaagc cgtccatacc atccatcata atatctacca ctgcaatatc
     4621 gacacgctgt ttttctaaaa gttttaatgc agcttcacca cttggttgtg tgtatgcatc
     4681 aatgtgctct gtttgtaaat ggctagctat ataatttagg attcgaggat cgtcatcgac
     4741 aacaagacat tgcaccatag ctataaactc ccttatcttt ttcatttatt atacatgtaa
     4801 aatatttttg cgtaaaaaaa caattgttca tattgagttc atatttcaac cttatactga
     4861 cgctaaagaa gaaataggga gaagtgaatc gatatgaaat tagcgataaa agagattatg
     4921 ttttacaaat ttcgttatat tttaatcaca ttaattattc ttttattaag tattatggtg
     4981 ttatttatta gtggtttagc tcaagggctt ggtagggaga atatttcgtt atttgaacac
     5041 tttgataatg atgaatatgt tgttcaaaaa atgaaagagc cgcaaattga gaaatcgcaa
     5101 cttagtgata cgcaacagaa tcaaattaaa aaggtaattc atcaagaacc atataaaatg
     5161 aatattcaaa cattgaaatt atctaataaa gaacaagatg tgattacaat gaatgacgtt
     5221 aaacaacaac gcatacaact taaaaaaggt gattatccta aaaatgctca tgaggtcgct
     5281 attaatgata agttagctgc agacaacatt agagtcgggg atagattaca ttttaaaaat
     5341 aattcaacta gttatagagt ttctggtatt ttaaacgaca caatgtatgc gcatagttcc
     5401 attgtgctat tgaacgataa cggatttaat gcattgaata aggttaatac ggcattttat
     5461 ccagtgaaaa atttaacaca acaacaacgt gatgagctta ataaaataaa tgacgttcaa
     5521 gtggtgagtg aaaaagattt aacaggtaat attgcgagtt atcaagcaga gcaagcaccg
     5581 ttaaatatga tgattgttag tttgtttgct attacagcaa tcgttctaag tgcatttttc
     5641 tatgttatga cgattcaaaa aatatcacaa attggcattt tgaaagcaat tggtattaag
     5701 acaagacatt tattgagtgc gttagtttta caaattttaa cactaacaat aattggggta
     5761 ggtattgctg tgatcatcat agtaggacta tcatttatga tgccggtaac gatgcctttt
     5821 tacttaacaa cgcaaaatat tttattaatg gtggggatat ttatattagt agcgatttta
     5881 ggtgcctcac tatcatttat caaattattt aaagtggatc ctatcgaagc aattggaggt
     5941 gcagaataat ggcattagtc gttgaagata tcgtcaaaaa tttcggagaa ggtttgtctg
     6001 aaacaaaagt tttaaaaggt attaattttg aagtggaaca aggggaattt gtcattttaa
     6061 atggtgcctc tggttctggg aaaacaacat tgctaacgat attaggcgga ttgttaagtc
     6121 aaacgagtgg tacagtgctt tacaatgatg cgccattgtt tgataaacag catcgtccta
     6181 gtgatttacg attggaagat attggtttta tttttcaatc ttcacattta gttccttatt
     6241 taaaagtgat agagcaattg acactcgtag gtcaagaagc gggaatgacc aaacaacaaa
     6301 gttcaacaag agcaatacaa cttttgaaaa atattggttt agaagatcgc ttgaatgtat
     6361 atccgcatca gttatctggc ggtgaaaagc aacgtgttgc gattatgaga gcatttatga
     6421 ataatccgaa aatcatttta gcagatgagc ccacagcaag tttagatgcc gatagagcaa
     6481 caaaagttgt tgagatgata cgtcaacaaa ttaaagaaca acaaatgatt ggtattatga
     6541 ttacacacga tcgaagatta tttgaatatg cagatcgagt gattgaatta gaagatggca
     6601 aaataactga ttagtggctt gtaaagacgc taaatgttaa tgatttaaga catagtagta
     6661 taaaagttag ataacagaat acgatttggg tttacaaaaa agcacgtcac tattatataa
     6721 aatgatgacg tgctttagtt tatttatttt caatataaca attgtctaat caaggattaa
     6781 cgaatattta aagatagttt gacgcaatat tagaaacaac ctataataat agtttgtttg
     6841 tggattaact attataaata aaagcggcgt aaagacatat aaaccaacta cttgaacaat
     6901 ataacgttaa taacaatcta tactgataca ttacgcctag ataatcttta atgagcacat
     6961 gtaagaaaaa gtgatatggt gtatgacttc cgacaccatc gatagataaa cctaattttt
     7021 gggctagtcg taaggcgcgt aatacatgaa actgacttgt tacacaaaca attttaactg
     7081 cttcatgata caaattgttg atgatttgtt tagaatataa aaagtttgtg tatgtattta
     7141 tagagtgaga ttccattagt atatctgttt tatcaacacc atgtgcaatc aaataacgtt
     7201 gcatagctaa agcttcagaa attggttcgt ctggtccttg tccgccagat acaatgatct
     7261 ttgttgctga tgcttgttgt tgatagatat caagtgcacg gtctaaacgc gctgcaagca
     7321 ttggtgtgac aaattcggta aaaataccag cacctaacac aattatgata tcaacttctt
     7381 tgttgtatga tctatgtcta tatgatactg tccaaacgag ataacaaata aaggttagta
     7441 acagggaaag acataatata gctaaccaca tagacaaacc tttcacaata ggtgactgaa
     7501 tcgtacttat aaatagaagt gctgatgtgt agagtacaaa tttatatgaa aaagataata
     7561 attttttaat aaataagcga ctagaagtat gagaaaataa atatctatgt ttgaatagca
     7621 tgataatact gattattata aatgttacaa acatagacca agggaaagta taggtcatga
     7681 tgctatagat gagtgacaaa aatatcgata tgacaactaa gatgtagcat gttaaattta
     7741 acgtcagagt atagttgaaa attaacggac aaataacgat aagtataaat attaataata
     7801 aattcaataa catactgaca cctcgcttat aataaatatt aaatataaat gtagatgatt
     7861 taatttatta aagcaaggag aacgcagcaa catgtaagtc ttaatttgtt atattatata
     7921 tgggtcaata tttttgtgtt ttttagatgt gcgatacaat gattgctgag gatactttat
     7981 aaaaatagta attagggaga aataattatg gtaaaacatt tacaagacca tattcaattt
     8041 ttagagcagt ttataaataa cgttaacgca ttaactgcaa aaatgttgaa agatttacaa
     8101 aatgaatatg aaatttcatt agagcagtct aacgtattag gtatgttaaa taaagaacct
     8161 ttgacaatta gtgaaatcac gcaaagacaa ggtgtaaata aggccgcagt aagccgacga
     8221 attaaaaagt taatcgatgc taaattagtt aagttagata aaccaaattt aaatattgat
     8281 caacgtttga aattcataac cttaactgac aaaggtagag catatttgaa agaacgtaat
     8341 gcgattatga cagatattgc gcaagatatt actaatgatt taaattctga agatattgaa
     8401 aatgtaaggc aagtattaga agtcatcaat catcgcatta aaacatatag caatcataaa
     8461 taggtttttg aaggtgttca aatataagac taaatatgta tgtattgaac accttctttt
     8521 tagttataat tgagagagta ctttcataaa taatgtaata aggtacaatt ctaaagtaaa
     8581 gttaatcatt tcttgaattg tattcttggg ataatatttg taaaataata atggtaaaat
     8641 aatgaaataa tttttgtgat atacatataa caattgaaga ggaacagaat tgaggtgaaa
     8701 gtatgaaatc ttgcccgaag tgcggacaac aagcacaaga tgatgtacag atatgtacac
     8761 aatgtggaca taaatttgat agtcgtcaag cattatatag aaagtcaacc gatgaagata
     8821 tacaaactaa taacatcaag atgagaaaaa tggtgccttg ggcaattggt tttttcattt
     8881 taatcttaat tattatattg ttctttttac taagaaactt caattcacct gaagcgcaaa
     8941 ctaaaatatt agtgaatgcg attgagaata atgataaaca aaaagtagca acattattaa
     9001 gtactaaaga taataaagta gattctgaag aagcgaaagt atacattaac tatatcaaag
     9061 atgaagttgg gcttaagcaa tttgtcagcg accttaaaaa tacggtacat aaattgaata
     9121 agagtaagac cagcgtagct tcttatattc aaaccagatc tggtcaaaat atattacgtg
     9181 taagtaaaaa tggcacgcgt tatatctttt tcgataatat gagctttact gcacctacca
     9241 agcaaccaat tgttaaaccg aaagaaaaaa caaaatatga gtttaaatct ggtggtaaga
     9301 aaaagacggt tatagctgaa gcaaataaag tgacgccaat aggtaatttt ataccgggga
     9361 catatagaat tccagctatg aaatcaactg agaacggtga ttttgcaggc tatttaaaat
     9421 ttgattttag acaaagtaat tctgaaacgg tagatgttac tgaagatttt gaagaagcaa
     9481 atataactgt aactttaaaa ggcgatacaa aattaaatga tagttctaaa aaagtaacta
     9541 taaatgaccg tgaaatggca ttttcaagtt ccaaaacgta tggtccatat ccacaaaata
     9601 aagatattac catttcagct tcaggtaaag cgaaagataa aacatttaca acacagacga
     9661 agacgattaa agctagcgat ttaaaataca atacagaaat aactttgaat tttgacagtg
     9721 aagatatcga agactatgtt gaaaagaaag aaaaagaaga aaacagcttg aagaacaaat
     9781 tgatagaatt ctttgctgga tattctttag cgaataatgc tgcgtttaat cagtcggatt
     9841 ttgattttgt atcatcatat ataaaaaaag gatcatcttt ttatgatgat gtaaagaagc
     9901 gtgtatctaa aggaagttta atgatgatta gttcaccaca aattatagat gctgaaaaac
     9961 atggtgataa gattaccgca accgtaagat taataaatga aaacggtaag caagtagata
    10021 aagaatatga gcttgaacaa ggctcgcaag accgcttgca attaatcaag acatcagaaa
    10081 aatagagtat aaaacttaaa taagatgcat gtgtcatgga atacaaacaa catcctttca
    10141 aagtatttac aattagtgtg aattactttg aagggattgt tttttgtgga aaaatttatg
    10201 aagtgctgag gtttatatca ttttatacct tttataagtt gtaatgatgt tcatgaaact
    10261 tgtgactaat gctaatatat aaaagagcat atttaaattt agtttctgtt cgtgatagtt
    10321 ataaactaat cattatataa aggagatagt catatgaatg atttaacgca ttttaacaag
    10381 cgttcaccga tatttattat tattttagga tctttgactg ccattggagc attatcgata
    10441 gatatgtttt tgcctggatt accagatatt agacatgatt ttcaaacaac gacgtctaac
    10501 gcacaattaa cattatcgat gtttatgatt ggtttagcgt ttggtaattt gtttgctggt
    10561 ccaatttcag attcaactgg gcgcagaaag ccgcttatta tagctatgat tatttttaca
    10621 ttagcaagtt taggtattgt ttttgtacat aatatttggc tgatggttgc attaagattt
    10681 cttcaaggag tgacaggtgg cgcagcagca gttatttcaa gagctatagc aagtgatatg
    10741 tatagtggta atgaactaac taagtttatg gcattattga tgcttgttaa tggtattgca
    10801 ccagttgttg cgccaacaat agggggcata attttaaatt attctgtctg gcgtatggtc
    10861 tttgtcattc tgactatctt tggcttcgtc atggtcatag gttctttatt aaaagtacct
    10921 gaatcattaa cagtgacaaa tcgtgagtca agtagtggat taaagacaat gtttaaaaac
    10981 ttcaaaatat tattgaagac gccacgtttt gtattaccaa tgctcattca aggtatgaca
    11041 tttgtaatat tatttactta tatttctgcg tctccgttta ttattcaaaa aatttatggc
    11101 atgactgcaa tacaatttag ttggatgttt gcaggaattg gtattacgct cattatatca
    11161 agtcaactta ctgggtacct tgttgatttt atagactcac aaaaattgat gagaggcatg
    11221 acgatgattc agattatcgg tgtaatttta gtgacgattg tattgcttaa tcattggaac
    11281 ttttggattt tagcgatagg atttatcatt ttaatagcac cagtgacagg tgttgcgaca
    11341 cttggtttta caatagctat ggatgaaagt agtagtggta gagggagttc atcaagttta
    11401 ttaggtttag tgcagttttt atttggtggt gttgcatcgc cattagtggg ggtaaaaggt
    11461 gaagataatc ctatacctta cataattatt attatagcta cagcagttat attaattatt
    11521 ttacagattt ataatatgaa ggtatttaaa acaaatcgat gataaacata gtttcttcta
    11581 ttatattgaa ttgaaaaatt aaaaaacaaa acttgagcta ttcatagacc tatgaattta
    11641 gcatcaaaaa taaaaatgcc gagattatta tgtggaaatc tcggcatttt tatattgata
    11701 gcaccgtaac atatgtacta ttatctatcc atttggttta actttatcta gttgttttat
    11761 agcatttctt tattagaatg aaaccgtatt attagttttg aagtttaatc gtattagcca
    11821 ataaaatgtc agcaacttct tcaggtgatt cattacaacc attcgaaagc caattaataa
    11881 acaccccagc ttgaccgcct atgatatatg taacataaag ctgtttattg gttactgtag
    11941 tactatagtc attcataatt tgttgatagt aatctctcgc taaatttata tagtctatga
    12001 ataatgtttt cattggatag gtaactaaaa tgtctttgaa aaattgctca ttgtgcttga
    12061 tatacttagc aataataatg aagaatttat aaagtttaag cttatcctgt ttaatatttt
    12121 cgaaattatg atgtagcgcg cttagaagaa attggaattt ttctttatgc tttgagttga
    12181 tggtatccaa taaatcaaat ttatctttgt aataatcata aaaagtagaa cgattaatat
    12241 tacaatacgc gcaaatcatt ttagtggata tttcttgaaa aggatattca gccatcaaat
    12301 caacaagtgc gttttgaatt tctattttgg cttgtcgttt catattgtta cacctttgtg
    12361 aattttaacc aacactctta taaaaattgt tggtgttttt ttagttatca tgtctctata
    12421 ataaataaag tgaattttaa aagcaaatag gaggaaatgt ttcatgaaaa agatgatatt
    12481 aattaatgtc attacggttg ttgtactgtt agcaattggg attgctggct tttatttctg
    12541 gaataaaaca acaagctatg taacaactga taatgcaaaa gtaaatggcg atcaaattaa
    12601 aatagcaagt cctgcatctg gacaaattaa atcacttaat gttaaacaag gtgacaaact
    12661 cgataaaggt gacaaagtag caactgttac tgtacaaggt caagatggcg aaacgaaaga
    12721 tatggatctt aaaatgcctc aaaaaggtac aattgccaaa ttagatggta tggaaggttc
    12781 aatggtgcaa gctggtaacc caatcgctta tgcatacaat ttagatgatt tatatgtaac
    12841 agcaaatatt gacgaaaaag atattaaaga tgttgaagta ggtaaagatg ttgatgtgac
    12901 tattgacggt caaaaagctt ctattaaagg taaagttgat agtattggaa aagcaactgc
    12961 tgcaagcttt tcattaatgc catcatcaaa tagtgatggt aactacacta aagtttctca
    13021 agtaatacct gttaaaatca ctctagaatc agaaccgtct aaacaagttg ttcctggaat
    13081 gaacgctgaa gtaaaaatcc ataaaaatta agggggtcat taaatgacta cgaccttcat
    13141 tattagctac attattttag cgctcattat tgttggggtt atcaatttat ttttaataag
    13201 atcaagaaaa aaaggcaaac gccaacaaaa ggaacaacaa tttacgacac gtcaatcaaa
    13261 tcaatctaaa tttaaagcta gtgatttaga caaaacaact gatcaatcga cacaacgtat
    13321 gacgcatgaa gagttgcgtg ttgacaatca agatgatcat agccaagtta gtctaaatgg
    13381 ttacacaaag gggtctgaga aagatcaaga agcattcact aataataatg gtgaggaagc
    13441 agttgctgct aaaaatcctg aatcagaaga atataaagtg aatgaaaaaa taaaaaaaga
    13501 acataaaaac tttatttttg gtgaaggtgt ttcacgcggt aaaatattag cggcattatt
    13561 attcggtatg tttattgcga ttttaaacca aacattatta aatgttgctt tgccaaaaat
    13621 taatacggaa tttaatattt ctgcgtcaac agggcaatgg ttgatgacgg gattcatgtt
    13681 agtaaacggt attttaatac caattacggc gtatctattt aataaatatt catatcgaaa
    13741 attattttta gttgcgttag tactctttac aattggttca ttaatctgtg cgatttcaat
    13801 gaatttccca atcatgatgg ttggacgtgt attacaagcc attggtgcag gtgtactaat
    13861 gccattaggt tcaattgtta ttattacaat ttatccacct gaaaaacgtg gtgctgccat
    13921 gggtacaatg ggtatcgcaa tgattttagc acctgcaatt ggtccaacat tatcaggtta
    13981 tattgtacaa aattaccatt ggaatgtgat gttttacggt atgttcatta tcgggattat
    14041 cgctatttta gttggttttg tatggtttaa gttatatcaa tatacaacaa accctaaagc
    14101 tgatatccca ggtatcattt ttagtacgat tggttttggt gctttgttat acggcttttc
    14161 agaagcgggg aacaaaggct ggggttctgt tgagatagaa acaatgtttg cgattggtat
    14221 tatctttatt attctatttg taataagaga attaagaatg aaggcgccaa tgttgaattt
    14281 agaagtattg aaattcccaa catttacatt aacaacaatt attaatatgg ttgtaatgtt
    14341 aagtttgtat ggtggtatga ttttattacc aatttactta caaaatttac gtggtttctc
    14401 agcattagat tccggattgt tattattacc tggttctcta attatgggtc tactaggacc
    14461 atttgctggt aagttgttag acacgattgg tttaaaacct ttagccattt ttggtattgc
    14521 tgtcatgact tatgcaacat gggaattaac taaattaaat atggatacac catatatgac
    14581 aatcatgggt atctatgtac ttcgttcatt tggtatggca tttataatga tgccaatggt
    14641 aactgcagct attaatgcgt taccgggacg acttgcctct catggtaatg ctttcttaaa
    14701 tacgatgcgt caattagcag gctctatagg tacagcaatc ttagttactg taatgacaac
    14761 acaaactaca caacacttat cagcttttgg ggaagagtta gataaaacga atcctgttgt
    14821 acaagatcat atgcgtgaat tagcatcaca atatggcgga caagaaggcg caatgaaagt
    14881 gttactacaa tttgtaaata aactagcaac ggttgaaggt attaatgatg catttatagt
    14941 tgcaacgata tttagcatca tcgccttaat tttatgttta tttttacaaa gtaataaaaa
    15001 agcaaaagct acagctcaaa agttagatgc agataatagt atcaatcatg aataaataaa
    15061 ataaattaat tgaagtgtga ctaatcaaaa attatgttgt ggggacatga tttttaaagt
    15121 atcggtgcca aatatggtta tcgatacttt ttttatttgt tgatttgtag aatgttagag
    15181 gaattatatt aaaatttggc attgacgtag taggtcatta ataaagaaaa agcaggaagt
    15241 gggtcaacga aatgaatttt gtgaaaataa catttctgtc ccaatcccta ctatataaca
    15301 ttattttaaa cggggcatgc gattacggaa gaataagctt ataacaagta aaccgatgct
    15361 acagcccagt aaaatgatgc cgttatgaat agcgtcagct tctgtaatca cttgatctgg
    15421 tggtacattt aaataatatt tttttaaagc atctgcaatt tcttgtgcga gtgctttaat
    15481 aatataactg aaatacaatg taatgactaa cataattgtt aacactaaaa tatttactac
    15541 ttttagaact gtgcgattaa atacaattgt cagtattgtt atgactaggc taacgataca
    15601 taaaataaag aaaccgttaa agactaaaat taagcgatcg attccttgtt gtaaatcatt
    15661 gatattaccc aaattaatat ttaattgatg catttttcca attgtctcgt taaaattttt
    15721 aatgccatca aaatctacag attctttggt gaagacaata ttaaaaatgg gttgtttcca
    15781 catgctgtat ccagcgattg cggttaatat aacaacaatg atttgaataa tcatgcttac
    15841 caatgaacgt tttttcttac catatcgctt accacgatga atattcgatt gttgaaatgt
    15901 ctcatcaaat ggttctaatc gttgatttgg atttgtcatg caaggtacct ccgtataaag
    15961 ttcttttctt tacttatcaa tatgtctagt tgtctgttat aatgatgaaa aatcactgtt
    16021 caattgtagt cttaaatttg aaagtgaaaa agaaaatatc ttctacatta tatacttttt
    16081 ttggcaaaga atgtagggga tagacaatca ttggatacat aaaatatata aaggtaggtt
    16141 aagacattga ttagaaaccg tgttatgaat agcgttgtca ataaatattt gcttcacaat
    16201 cgctcgatta tgtttaaaaa tgatcaagat gttgaaagat ttttttataa aagagaaatt
    16261 gaaaatagaa aaaaacataa gcagccttca acattaaatg ttaaagcaaa tttagaaaaa
    16321 ttatcattag atgatatgca agtctttcgc tttaatttca gacatcaaat tgataagaaa
    16381 attttatata ttcacggtgg attcaatgca ctacaaccat caccgttcca ttggagattg
    16441 ttggataaaa tcactttaag tacattatat gaggttgtac tgcctatcta tccaaagaca
    16501 ccagagtttc atattgacga tactttccaa gcgatacaac gtgtttatga tcaattagta
    16561 tctgaagtag gacctcaaaa tgtcgtagtc atgggtgatg gttcaggtgg tgcactggca
    16621 ttatcctttg tacaatctct tttagataat caacagccat taccgaataa attgtattta
    16681 atctcaccaa ttttagatgc aacactatct aataaagata tttcggacgc cttaatcgaa
    16741 caagatgcgg ttctaagtca gtttggtgtc aatgagatta tgaaaaaatg ggcgaatggc
    16801 ctaccattaa cagataagcg catatcgcca attaatggca caatagaagg attgccacca
    16861 gtttatatgt ttggtggtgg acgtgaaatg acacatcctg atatgaagct atttgaacaa
    16921 atgatgttgc aacatcatca atacattgag ttttatgatt atcctaagat ggtacatgat
    16981 tttccaattt atccaattag acaatcacat aaagctatta aacaaattgc caaatcgata
    17041 gatgatgatg taacacaaaa taactaataa catataaatg acccagttac gatttcatgc
    17101 aagatgatat ttgacatgaa atcgtaattg ggttttgttt ttgaaaatat agcaagggtt
    17161 atatagacta attgttaatg agcttctgag tgaaaatcac caataagtgt attgatatgt
    17221 gtgatgataa agtattgccc tttaatttga taaaattgat ctaaaaaatt atcgaattga
    17281 cgtggaatat cactaagttc tgcaaagtta gctgttttat cataattgcg atgagcaacc
    17341 tcattttgaa atttatcata atgttctagg aaatgttgat cttcaagctg ttgttccaca
    17401 atagtcataa tattatcgat ggcttgcttt tgtccttgaa cataagccat atgcacgcca
    17461 cgcttaagta gctgatttgc tggtccgtta atgtataacc ctttttgtgc atttaattct
    17521 gttaattcaa acttaacatc atctaataaa tctagtaatt cgatatgaat cttttgttgt
    17581 aatgcatctg tcattttaat gctcctttgt gtggaattgc tattttatgt cctaattgtt
    17641 caggccaagt gatagttggt tgatttttat aatagtgtgc tatttgtgtt gaaaatgatt
    17701 caggaaaagc atcagaacga cgtgtgtgct gattaattcc catcatcatt acttcatttg
    17761 ttgatgctag tgtaccatct tctttagtta atgttaaaaa taaatgcaac cgcttataat
    17821 cgtaatcata aatatataaa gtaacagtaa atacatcgcc aagagacaat tctaagaggt
    17881 atgtcgtatg ttcttctagt gtaaatagcg tatatgctaa attttcgcgt tcttttaaag
    17941 aaagaccgtg gctgtaatta aaacgattca cgacgtcact aaaaatgata ttataatttg
    18001 catcatgcat atgattgtta tggtcaatga attcagatgt tacggtttga gtatgtgtaa
    18061 aaagttgttt gataataacc atctccttag tgactatcat actaaaataa attaagacat
    18121 acacgttatt taattcatga taaaataata gagaattcgt tatttagggg gagtatcgat
    18181 ggctttaaag atacggtatc aaactacata tgaaccgttt aaagtagttg atgatatcaa
    18241 agagatacca aaagatgcga ctattgtttg gtatgatttt gatgaaccga atgaacaaga
    18301 aaatgaatgg tttaaagcac attttaattt taatgattta gaagtggatg atgcaattaa
    18361 cggtatgcca cgagctaaat ataaatctta taaagattat caatacttag tattccatag
    18421 tattatgggg tcaaactatt caccaatagc actaaatatt tttatacaag acaatgtatt
    18481 agtaacatat catcatcaaa cgttggaatc attaaataaa gtagtctata agtatatgaa
    18541 tacattagat gcagaattag attgtgcaga tgtcgtcata ttaattttag atatgatggt
    18601 tgataaatat ttcaactttg tatatgcact tgaagatagt gtttatcatt ttgaagaccg
    18661 ccatgtagat gatcgcttta ataaaatggt catggatagt gtctttaagt tacgttcgga
    18721 tttgattaaa gtaaaacgtg tactgtttcc tatgcaagag ctaattgata caatgaaaca
    18781 aaatggtgat ttgattatag ataataaaca ttcgctatac attcaacata tcgatgacca
    18841 cttgattaag caacgtaata tcattcgtac tgctcaagaa atgacaaacg aaatacgaga
    18901 aaattttgag tcatacacgt catttagaat gaatagtatt atgcaagtat taacgttagt
    18961 atcagttata ttttcaccgt taacattcat agctgggatt tatggtatga actttgtaaa
    19021 tatgccggca ttacatttac attatggata ttatatttgc ttagctgtaa tgtttgttat
    19081 agctgtcgta ttaatcatct ttttcagacg taaaaaatgg ttttaaatta gaaatgacga
    19141 aaaggtaggt ttaatcatga gtgattttca aagagaacag agaaaaaatg aacatgttga
    19201 aatagcaatg gctcaatctg acgcaatgta ttcagatttt gataagatgc gttttgtgca
    19261 tcattctatc ccatcaatta atgtaaatga tatcgatttg acatcacaga cgcctgactt
    19321 aacgatggca tatccggttt atattaatgc aatgacgggt ggtagcgagt ggacgaaaaa
    19381 catcaatgaa aagctagctg tagttgcaag agaaactggc ttagcgatgg cagttggatc
    19441 aacacatgcg gcattgagaa atccacgcat ggctgagacg tttacgattg cgcgaaaaat
    19501 gaatcctgaa ggcatgattt ttagcaatgt tggtgcggac gtaccagtag aaaaggcttt
    19561 ggaagcagtt gaattacttg aggcacaagc gttacaaatc catgttaatt ctcctcaaga
    19621 attagttatg cctgaaggga atcgtgaatt tgtgacttgg ttagataata tagcgtcgat
    19681 tgtatcacga gtgtctgttc cagtcattat aaaagaagtt ggatttggta tgagcaaaga
    19741 attaatgcat gacttacaac aaataggcgt caagtatgtc gatgttagtg gcaaaggtgg
    19801 tactaacttt gtagatattg aaaatgaacg tcgtgcaaat aaagatatgg attacttatc
    19861 atcatgggga cagtctacag ttgagtcatt acttgaaaca acggcttatc aaagcgaaat
    19921 ttcagttttc gcgagtggtg gtttacgtac accactcgat gcaattaaaa gtttagcact
    19981 tggtgcaaag gcaactggta tgtctcgtcc gtttttaaat caagttgaaa ataatggcat
    20041 tgcacataca gtagcttatg tagaatcatt tattgaacac atgaaatcaa taatgacgat
    20101 gttagatgct aaaaatattg acgacttaac acaaaaacaa atcgtattta gtccggaaat
    20161 attgtcatgg atagaacaac gtagcttgaa tatacatcga ggataaataa aagaggaggc
    20221 gaagacctcc tcttttattt atgagtttga tgtttaacta aaccattgaa tgaatcccat
    20281 aatgactata acaccaatta aatcgattaa gaatgcacca acaataggta caactaaata
    20341 tgctttaggt gagttaccat attttttagt aataacatct aaatttgcca tggcatttgg
    20401 cgttgcacca agcccatgcc cgataaaacc acctaccatt actgcagcat cataatcttt
    20461 tcctaaacct ctaaataaaa ttaaaacagc aaataaaatc ataacgacaa cttgaactaa
    20521 aacaataata ataagaggta tagcaagttt ataaatttcg attaattgaa tgctcattag
    20581 cgcaagagat aagaaaatac ctaatgcgac atcgccaatt tgattagtaa tttttaaatc
    20641 tacaatatta aaattaaaac tttcagagat atttcggaca ataacagcta caaataatga
    20701 gccaacgtaa attggaacat taatccctgt ttgagatgta aacaaatgac tgaaataact
    20761 tccaactgcc atacagaata caacgattgt aaattgaatg aagaatactt cagtggcatt
    20821 aaatttacta tgtaaatgtt cgttatatgc tacttggcta taatctttaa atgtatcgtc
    20881 actatgttga ggctttaagt tataacgttt gattaaaaat tttacaactg gaccaccgat
    20941 aagccctcca aatacaagac ctaaagttgc agctgcaaga gccgctgtca gtgccgaatc
    21001 aatacctaaa tcttgaattg tcttaccata agcagcagca ttaccatgac cgccttccat
    21061 agacatggaa cctgctgtta atcctaacaa aggtttaata tttaatactt ttgctagtga
    21121 tacaccaact atgttttgaa tgactgaaat gatagcacaa aacataaagt ataatagcaa
    21181 gagtttgcca cctaatttaa ataatttcaa tgatgcacca agaccgattg tcgtaaagaa
    21241 tgctaacatg aagaaatctt gaatgaatga agcatctaat ttaatcttaa ccatgccaaa
    21301 tgaatccaaa gccgcaacta aaatagcaaa gattaagccg ccaatcactg gtgctggtat
    21361 acaaatacgt tttaaaaaat taacgtgatt aacgatagcc ttaccaagta aataaaggat
    21421 acaagctaaa cataatgttg taattgcatt aagttctatc attttcaaat cccccttgtt
    21481 gaatatccct ctccctaaat aagcttttat atgtaagcgt tataaaaggg cgcttttaac
    21541 attatacatg caacaaatca atatttctag tttttgaaaa taatatcaat aatattaatg
    21601 catctaaatt taataattat aaataattct attgttatat tttataatta agaacaagtt
    21661 caataagagg agatgaatca aaaaatatga tggatacaaa gaaaaacaat caacccatcc
    21721 gcatgttgac ggcacgattg attgacttat aaggagatag atttcatttt catgatagta
    21781 atcaatgaaa gatggcattt atttccaagt atcttcggga aacatacaat ctgatttacg
    21841 catgcgagac acaaatggat tacctttcac tgtgtaacgt aaagatttat gtgtccaatc
    21901 acctttattt ggaataccga ttcgtggact agcaataata tctttaggat atttacgatt
    21961 cttagtatca atagacaatc tacagtcatt taacgtagcg ccatcgatag cccgtggaat
    22021 gttaaatgcc ttagtccatt ttcctgggcc attcgttacc tcgtagcctt tcttacctct
    22081 gttacggaac atagcggata aaccttcttc tggttcaatt gcgcgtataa gtacgccttc
    22141 aggtatacct tcagattttg ttacaaaatt aatgagtaaa tgcgtatgca tgacatgtgc
    22201 ataaattgta ccaccacgtt tatataatga cgtgacttta ggtgttattt taccgccata
    22261 gccatgagcc gcacgatcat tcaaacctaa gtaagcttcc gtttccacga tgtagcctgt
    22321 atacgtttga gtggtatcct gataaatcac tttgacacct aataagtttt tagcaatttg
    22381 tcttgtatca ttattaacga aatccataat attctcctcg caattatatg attaaattaa
    22441 ataaaaatga taaagatatt tttattgtgg taaatgtttg ataaaatagt attgttaaga
    22501 attataaaaa tgtaaagtat atactttgtt atggaatatt ttatttgcag agattgagac
    22561 tcttttaaac gtgaacattg cagtgtgaca tatttttagt atagttaacg aagcgttgac
    22621 tttcaaatga gtctccatat agatgtgtac ttgtagcggg ttttcatttt actagtgatg
    22681 aaatataaag ttcattttcc aaagtgattg acgattgaaa tgaagtggct tgataatgaa
    22741 tcttgtaaat tataacggta acaataatta aaaatataaa tatgattaac gaaagggagg
    22801 ttacaatgga acaattacct caagtaggtt ttatgaaatc attcgtatta ttttggaaaa
    22861 attatgtaaa ttttaagggg agatcaagac gtagtgaata ttggtatatg gcactatggc
    22921 atttgatact tatggttcca gctgtgtgtg tgctaataat aaacctattt ctattcttta
    22981 tttctatagc ggcagataac gaaacattaa tagttatgag tatattgatg attatcctta
    23041 catctgtgta tacttttgtc tatagtttgg caacgtttat tccaaattta gcgttgtgcg
    23101 ttcgaagatt tcacgatata tcaagaacaa tgttatttcc aatgattatg acagtattca
    23161 gtattatatt ttatatagta ttacagatta tcgatagtta ttatgacagt gattttacat
    23221 taatgccgat gggtcttaat attttgttag tacttcttta ttttgtttat ttaggcctaa
    23281 caattgttat gatagtattc ctatgctttg acagtaagcc ggcaaataaa tatggcgaaa
    23341 gcccaaaata cccgtctaca attaaaaatc aaccagtgac atctgaaaca ccgaaaacac
    23401 cggaagaaac agataatcaa ttgtaatgat ataaatatct cggcacaatg atgctgggat
    23461 atttttttac aaataaggca ataaagatat gggaaatgcg tatctagcgc taatgatact
    23521 taacatgcaa agaagatact atttaaatat ttcaatttca gaaattttaa aaataaattc
    23581 actttaaaaa tattgcattt atagagaata gaaatgttat attaagataa atacatattt
    23641 ttttaggagg caaagtatgg gacttgtaat tgaacatgtt acgaaacgtt ttggcaagat
    23701 gacggcagta aatgatattt cattagagtt agaatcaggc aaaatgttag gttttctagg
    23761 aagaaatggt gcaggtaaaa caacgacatt tcgtatgatt ttaggcttaa gtgaaccaac
    23821 tgaaggacac attacgtata acggtaaaaa actagataaa acaatgtaca atcgtattgg
    23881 ttatttaccg gaagaacgcg gtttacatgg gaaattgaca gttgaagaag aattgaaata
    23941 tttggcaact ttaaagggaa tgtcaaaaac agaaatacaa cagcaaatat cgtattggct
    24001 tgagcgtttt gatattactg aaaaccgcaa aaaacgaatt gatagtctat caaaagggaa
    24061 tcaacaaaaa attcagctgt tagcaagtat gttacataaa ccagaattac taatattaga
    24121 tgagcctttc agtggtctag acccagttaa tgttgagtta ttaaaagaag cagttaaaga
    24181 cttaaatgat tggggcagta caatcgttta tagttctcac cgtatggagc atgttgaaga
    24241 attatgtgat gatgtttgta ttttagataa aggtcaactt gttgtttctg gtgatatcaa
    24301 tcatgtacgt gcatctaatg gtaacaagaa agttgtcatt gaatcagaaa caacactacc
    24361 agatttaaca aacattagag gcatcattca tagtgaaaac atgaagcaag gtcttcagtt
    24421 gacgattgag aatgaagacg tagccaaaga tatttatcaa gttgtggcac atcaaggtta
    24481 tgttaaacga ttccaagttg tagaaccatc attacaagat atattcattg agaaggttgg
    24541 tggcaaggat gcataagttt tgggcgacat ttactttgac atatatcaaa aaaattaaag
    24601 ctaaatcatt tgtaattttt atgattatta ttgctgcctt aatgattggc ctatctaaca
    24661 ttgataagat tattaacatg tttgatgatg gtccagataa aattggtgta gcagcaccga
    24721 atgaacaaat ctataaggtt ttcaagcaac aggcgaatac atttcatagt gatgctaagt
    24781 ttactaaagt ttctatagaa gatgctgaaa aagaagtaaa aaaacataaa cttgataaag
    24841 cttatattat caaggtgaat caaaatcgca cgttacaagg gacaatcatt agtgagaagc
    24901 gagtaagtca tgaagattca caaaaagtac aagcactgct tacagctatt caaacaaata
    24961 tggtagccgg ggaattaaat atcaacaaag aagacttaca aaaattacaa gcacaaagta
    25021 aagttgataa taaggtgatt tctaatgatg aagtcgataa agtatcagaa ggacaaaaga
    25081 ttttcaacta tgctttagct tatggcatta tctttctaat gttctttatt gttttaaatt
    25141 atgcatctca aatagctatg gaaattgcta gtgagaaaac atcgcgtgtc attgaaatga
    25201 ttattacaag tatttcacca atccaacata tttttgctaa aattttaggc gttatagcag
    25261 ttgccgtgac tcagattatt ttaattgtat tgatggcagt tatatgcatt tttgcctttg
    25321 acttaaaaga cttgctacaa ggttttaacg tggaaatgaa tcaattgtct tggcaaatta
    25381 ttattgtcgg aattataagt agtattgttg gaattatggc gtatgtatta cttgcagcca
    25441 tcctcggatc attaacatct agaatagaag atttaaatca atctttgatg ccactgacat
    25501 tacttggtat gattgcattt tatatcgcga tattcacgat aaataatcct gatgggcgac
    25561 ttgctttaat cacaagttat attccgtttt tagcgccatt tcaattagtt gtacgcgctc
    25621 aaacttcagg gcttcaaata tatgaggtca tcttaagttc tttaatctca attgtaatgg
    25681 tagctgtctt gatatggatt gcaattaaaa catataaaga ttcagtactt acatttgaac
    25741 gtggcttatt taattcaatg aaacgtgtat ttaaaaaata aaattgatga gagtgacaca
    25801 catttatcat ataaaatgat gatgaaagcg tgttactctt atttttttat atttagctat
    25861 agaataatgt ctaaaaaagt gtacaatatg aatgtgaaaa cgaacttttt aaaatgggaa
    25921 tgtgaagtaa gaagaaatgt aaacaaatga tatgtagccg atagattatt ggtagcaaat
    25981 gtgtgatgga gtgatttcat gttatatccg atttttatat ttatattagc tggcttatgt
    26041 gaaataggtg ggggatacct gatttggctg tggcttaggg aaggacagtc ttcacttgtt
    26101 gggctaatag gcggtgcgat actcatgtta tatggtgtca ttgcgacatt tcaatcattt
    26161 ccatcattcg gaagagtata tgcagcatat ggtggcgtat ttatcatcat gagtttgatt
    26221 tttgcgatgg tcgtagataa acaaatgcca gataaatatg atgttatcgg tgcaatcatt
    26281 tgtattgtag gtgttttagt aatgttgcta cccagcagag cttgaacact tgtggttaga
    26341 agggagaagt aaaaatgata gttgaagttg agcaccaaag acaaagtgta gatttaatca
    26401 aaattgataa tgatgaaact aaaattattt ttacgaatta tggagcaaga gtagtgtctt
    26461 ggaaatatca tgataataat attgttcttg gaaatgttgt tgaagcggat gaattttatc
    26521 atagtaatcc ttttaaattt ggtgcttcta taggaagata tagcggtaga attgataatg
    26581 ctaaatttaa gttgaaaggt aaagaatatc agttagaaaa aaacaacggc gaacatcatt
    26641 tacatggtgg atgtcatggt ttggacaata agttatttga ttatgaaatt agaaatgaaa
    26701 ttgctcaaat taaagtgata tttaagacgg tgttgaaatc agctgatgat catttcccgg
    26761 gtgatatcga tgtgacgatt acacatattt atgatgcgga tcaccaatgg tcgattgaat
    26821 acgaagcagt tgcatcagaa gacacattgt ttagtcctac gaatcatgtg tacttcaatt
    26881 tgaatcgaga taataatgtc gttgataatc atcgcatttc aagtaatcaa ttagatatgt
    26941 atgtattaga tgagcgaaat attgttacag gtgatattct agatttacat gaagtgtttg
    27001 aagataacaa gataaaattg agtgatattt ttacatcaca acatgcgcaa ttaagccaac
    27061 aaatgacacg ttttggtggt ctcgatcacc catttacagt tggtgaacat aaaatgtatg
    27121 ttgaaaatca tgaatttatg ttagaggtcg atacagatat gcctcatgtt gtgttcttta
    27181 cttttaacca gcctgatgag tgggatagtc catttaatat ttataaacca cattctggct
    27241 ttacattaga aacacaattt ttaccaaatg atattaattt atatggtgaa aaagcaccgt
    27301 caattttaaa agctggtgaa gtgttcaatt cgaaaacaag tttccgtatc gaagaaaaag
    27361 tagaaaatga ttaaaagtat cggcatttat ataatattta tgttatttgg cggaattttg
    27421 aataggttat caagaatcgt atcaaatgaa gtgacatcat taatatacag tttgaaataa
    27481 agaaaatata taacataaaa aaggaatcaa tagcatcagt ggttatagat aagactatta
    27541 tacaaacgat gtgtaagttg attccttttt gttttgaaaa gttcatttaa cacttataat
    27601 gaaaggtaat tatttcaatg ttctgttata cattttttga agtttatcgc gtcttgttgg
    27661 tgacagaaat ggattgttta gtgcatatcg taacatatct tgattttgtc tatcattata
    27721 ataaagttca ttaagctgtt gtacagataa acgatgttcg attggtgctt cttgtattaa
    27781 gtaaagctga tcatttggag atacatagcc ttgttttaat acccggaaat agaaacctgt
    27841 tttaccagat gtagacatgc gcttcactaa atcaggaata ttatattttg cttgaatttt
    27901 ccagcatggt tctctaattt cagaaacctc aattaacgct tcacctaaac gatattgatt
    27961 accaaagtaa acatcagatt cgtctaaata atcaaatgtt aaattctctc caaacatcgc
    28021 atgaataggt agtgtaggta aatcatcttg atacagtgca taattatgct tactaaaccc
    28081 acaaactgct ttatgtggtc caccatgatc tttataagcc tgttcatcac cagtgaaacc
    28141 agttgaagat aaccatgttg tttgtgaaat cttagtttta tctaaagcag agcgcatcgg
    28201 tcttttcgag ctataaggca aatcttggat ttttcctgtc gaaattgcgt gaattgatat
    28261 catatcattc tccttaacga gtattattta aaatatattg tactataaaa catttttaga
    28321 attgtaaatt ctgattgata tgtttaaaaa aatgttagaa ataagggtat gttaatttat
    28381 acgagtcatg taaaatatga attgaataga ttacataaat atgggaggct accttcatga
    28441 aagatgtcaa agcactcaag ttaatgacat taaatgatgt tttaagtcaa atcaatggtg
    28501 acatgacgct gggaattggt acaggaagta caatggaatt actcttacct caaatggcgc
    28561 aactaattaa agaacgtggt tacaatatta caggtgtatg cacttctaat aaaattgcat
    28621 ttttagcaaa agaattaggt attaaaatat gtgaaatcaa tgatgttgat catatcgact
    28681 tagcaattga tggtgctgat gaagtagatc catctttaaa tataattaaa ggtggcggtg
    28741 gtgcgctgtt cagagagaaa gttatagatg aaatggcatc gcgttttgtt gtggttgtcg
    28801 atgaaacgaa aatagttcaa tacttaggtg agacgtttaa gttaccagta gaggtggata
    28861 aatttaactg gtatcacata ttacgtaaaa ttgaatcata cgctgatata aaagtagaac
    28921 gtcgtgtaaa cgaagatgtc gcatttataa ctgataatgg caattatatt ttagattgta
    28981 agttaccaaa aggaattgac ccatataagt tccatgagta tttaattcat ctgacaggtg
    29041 tgtttgagac aggatacttc ttagatatgg cagatcaagt tattgtaggc actcaagagg
    29101 gtgttaaaat tttagaaaaa taatatatag tatagaaaag cgcttgctag catcatcttt
    29161 ttaagaggta ctagcaagcg ctttgttatt atttgttttt atctgaagat tgttcttcag
    29221 ttttttcgat atgtttttct gcatcaatta caactgatga acgtctgtct tctttataag
    29281 tcgcaggttc ttttaatgat tgctctattt catcttcatt tgaatcgttt gttaaatcgt
    29341 cactttgtgg tgcttgttta actgattctg ctgattctac tgactccgaa gtgatgtcat
    29401 gatcttcatc atgattagat tcagtttgat tacctatgtg atgttgaggt tctgtttttt
    29461 cagtaccttc atgaatgctt ggttcgtcag ccactgtatt ttcagtatca tttttagcaa
    29521 caccgaccgt tgatgcagta gctacacctg ttttttcagc atctttaata tttgaagaag
    29581 atttttcagc ctcagtatga ttagtttctt catcgtcatt gacatggtct aaataattat
    29641 taggctcaag ttcttcaagg tttcgtcttg ttgttaaata tgcaatacct cggataatta
    29701 agcttaaacc aatgtatcct actgcaacga ttgctgttga aatggcgatg actttgattg
    29761 aaaatagatc gccgatttct tcgctaaaca agaaaataag tccgaatgtc attgaagcat
    29821 ggaatgtcgt tgcaatataa attgtacgtc ctttagtcgc tctaattaat tcaccaagaa
    29881 tcatagagaa tgagaatgta taaaggaagt tataagcagc aaattctgta ccataagttg
    29941 tatttgctga gaaaactgaa tacatcaaac caacaacaat acttgcaaag aacgtgttca
    30001 ttttcgtttc aacaatattt tgtaagtatg agcggaatcc gaattctact acgaacgcca
    30061 tcagaatatg tccaatcaga atgtgtgtaa taggtactga taagcctgtt gattgtaata
    30121 aaataaagct atctgcaaat gtattaaagc tgtacatacc aataattaga ataattaaag
    30181 gtaaaattaa agctagcaat agacgttcaa ttactttcag gctgattgag aattttaaac
    30241 ctgcaagttg gaccttttta tatttgaaaa cgagtataca aatgattgca gcaattaatg
    30301 gtgctagatc tgtaacttca aatataaagt gtttgacacc aattatagac tggaaatctc
    30361 tgagcataat ggataacgcc attgtaatga caaagaagac gaaaatcgtc attgcccatt
    30421 gaaaaccaga aattttattg ttcttcatta tatgtaacct ccattaggta acaagcaaaa
    30481 tatcttttag taacaattat acatattaca agccgagaga taaattgtct atgtcaatat
    30541 taatgaaaat gtaatagtct tgacgtattt tattaacata aatcagctaa gtattcgtta
    30601 ttacatagaa aataggggta aaagtaatgt agataagtat ttttaaggta cggatttaac
    30661 tatgaattga attcaaaatt tcctatacgt tatactttaa ttgttaataa gccacataaa
    30721 taagaggggg aaatgctgtg tacaagcaag gtgaaccaaa tttatggact ggaaggttag
    30781 atagtgaaac agacccgaaa aaatttagac attttcaaac agtaacattt gaagatttgt
    30841 ctaagctgga aaagagtagt atgccatcag gggtcggtat attaggctat gctgttgaca
    30901 aaggtgttgc tttaaacaag gggcgcattg gtgcaaaaga agggccagat gcgattaaac
    30961 aagcatttgc aggtttgccg gatttgaatc aatgtgaaac tttagtcgat tacggaaatg
    31021 tttatcatga tcatgaggaa ttaattgata ctcaaaaaga atttgctatg cttgcagcga
    31081 agtcaattgc taatcataga caaacatttt tattaggcgg tggacatgat attgcgtatg
    31141 cacaatattt agctacgcgt aaagtttatc caacacagtc aatcggtgtg attaacattg
    31201 atgcgcattt tgatactcgt gctgaacaag aatcaacatc tggtacgagt tttagacaaa
    31261 ttttagaaga ggatgacaat actgattatt tagttcttgg tatagctcaa ggtggtaata
    31321 cgcaaagttt atttgattat gctaaagaga aaaagattga ttatgtcttt gcagatgaat
    31381 tattaaacca tgtgtcgcca cctattaaag atatgattga gcgttttgtg catgaacatg
    31441 acgtcattat gtttacgatt tgtatggatg tcattgatag tgcatttgca ccaggggtaa
    31501 gtgcgcctgc agtgttaggt ttgtatccac atactgttct tgaattagca aaacgtatta
    31561 ttccaagtga taaggtgtct tcagttagta ttgctgagat gaatccaaca tatgacgctg
    31621 acaatagaac tgctaagctc gttgctaatt tagtgcatca ttttttaaaa taattaaaaa
    31681 tatttatatg tgacgagatt tgcattgcag ggcagaatgt gagtatttct taatagaagt
    31741 tatattaacg ttgatatatt tgaaaaataa ttgcagtaag aagactctta agttgtagtc
    31801 atcttactgc ttatttatgt ttgttggtct gaatgtaatg atttatacat gtaaatcata
    31861 aaattggttg tgaaattgtt ttacagttga ctgagcaact gcataagagg ctctaataac
    31921 ttaagttaaa ggcaaaaggt atacagttga ctgagcaact gcataagagg ttctaataac
    31981 ttaagttaaa ggcaaaaggt atacagttga ctgagcaact gcataagagg ctctaataac
    32041 ttaagttaaa ggcaaaaggt atacagttga ctgagcaact gcataagagg ctctaataac
    32101 ttaagttaaa ggtgaaaggt atacagttga ctacgcaact gcataagagg ctctaataac
    32161 ttaagttaaa gagcctctaa aagtccatat aaataatctt ggcattgttg tacgaatgta
    32221 tgtacttgtt cggagtgttt gcgtttttta tggtaattga tataaatttt tcggcctaag
    32281 tttggacgaa tttttttata ttctaaatta gacgtgtgaa atgattggta ataaaatctc
    32341 ggaatgatag cgtaacctaa gccgagatga acaaatccaa ctgctgattc gaatcgatct
    32401 gtttctacaa cgacatttgg acgaatattt cttctattaa aatagtcatc taagtgtttg
    32461 cgcacttgag aatttttgtt tggtaatatg agtggcaaat tttcaacatc tacccaattt
    32521 tgatttttaa atgtttcctt gggtgctaat aaaatgtaag attcctcata taatggaata
    32581 gattttatat cttcgtgggt tattttttca tttgttatag ctaaatgaat attaaaattc
    32641 agtaattgct ctatagattg atgtttatca tgtatttcat ataaacgata ttgctgttca
    32701 gggtagtcgg aatggtgctt tcgaattaaa ttcgcaatcc attgattcgt agattcaaga
    32761 gtcccaattt ttatccttgg ttctgatgta acgcttaaat catacatttt ttccatcgtg
    32821 gatcgatatt gttgaactaa ttcgctcgca taacgataaa actgtattcc tttttcggta
    32881 atcttgatgt cttttgttga acgtgtaaat aagtcataac ctaaatctgc ttccattttt
    32941 ttaatcgtag cagttaaaga tggctggcta atatgtaaaa attgtgcagc tttagtaaaa
    33001 ctattatatt tcacgatagc caagaagtat tctaactgaa taattttcat gtgttttgct
    33061 ccttttattt atagattcaa gctatggata attagtttat ttatatttgt tggctatatt
    33121 ttaacatact ataatgaata ttgaagataa taatgtaagc gttttaaatt tagtggaatt
    33181 aacttctaaa aagaacagga gcgaatttta tgagaaaaat tcaagcaaaa aaaggtttga
    33241 gtattgagtg taaaggttgg gaacaagaag cagtacttag aatgttatac aacaatttag
    33301 acccagaagt tgctgaaaga ccagaagatt tagttgttta tggtggtatt ggaaaagcag
    33361 cacgtaattg ggaagctttt gaagcaatag agaaaacgtt acgagaatta gaatcagatg
    33421 agacaatgtt agtacaatca ggaaagccag ttgctgtatt taaaacacat gaagaagcgc
    33481 cacgtgtatt aatttcaaat tcagtattag tgcctgaatg ggcaaactgg gatcatttta
    33541 atgaattaga taaaaaaggt ttaatcatgt atggacagat gacagccggt agttggatat
    33601 atattggttc tcaaggtatt gtgcaaggta cttatgagac atttgccgag ctaggtaatc
    33661 aacactttaa tggagattta gcaggcactg ttacactgac agcaggttta ggtggtatgg
    33721 gtggtgcgca accgttagca attactatga atcatggggt agcaatttgc gtggatgttg
    33781 atgaaacacg tgttgataag cgaattgata cgaaatactg tgatgttaaa acagctgatt
    33841 tagatgaagc attaaaatta gcagaagagg cgaaagaacg tggagaagga ttatcaatcg
    33901 ggttagttgg aaatgctgta gacatccatc aagcgattct agaaaaagga tttaaaattg
    33961 acattattac tgaccaaaca agtgcacatg atccgctaaa tggatatgtg ccacaaggat
    34021 actctgtaga agaagcgaaa gtattgcgtg aaaaagatcc gaaaaaatat gttgaactgt
    34081 cacaagcttc aatggcaaag catgttgaat taatgcttga attccaaaaa cgtggcgctg
    34141 tagcatttga ttatggtaac aatattcgtc aagtagcctt caataacgga gtgaaaaatg
    34201 cttttgactt cccaggcttt gtaccagctt acattagacc attattctgt gaaggtaaag
    34261 ggccattccg ctttgctgcg ttgagtggtg atccaaaaga tatcgagcgt gcggatgaag
    34321 aaatgcgcaa acttttccca gaaaatgaaa agttattaag atggcttgat ttagctgaag
    34381 aaaaaatttc atatcaagga ctaccatcac gtattgcttg gttaggctat ggagaaagag
    34441 cgaaaatggg cttagcttta aatcgtcttg tacgtgacgg tgaaatttca gcaccaattg
    34501 ttattggacg agaccattta gatgctggtt cagttgctag tcctaaccgt gaaacagaaa
    34561 gtatgaaaga tggtagtgac gcggttggtg attgggctgt attgaatgca ctcattaaca
    34621 cagctgcagg tggttcatgg atttcattcc atcacggtgg cggtgttggc atgggatatt
    34681 cacttcatgc gggtatggtt gttgtagcag atggatcaga gcgtgctgaa agaagattgg
    34741 aacgtgtatt gacgactgac ccaggtatgg gtgttgcccg acatgttgat gctggctatg
    34801 acatcgctat tcaaacagct aaagaaaaag gtattcatat tccaatgatt gataaagcag
    34861 gtgataagta atgaatgatt taataattaa tcatatagca gaattaattt taccgagatc
    34921 aacagataaa cctttgaagg gtaaggaatt agatgaatta aatgttgtga aaaatggtac
    34981 agttgtcatt aaagatggca aaattgtata tgctggaaca catacagatg actacgatgc
    35041 gactgagacg attgatgcta gtgggaaggt agtgtcccca gcattagtag atgcacatac
    35101 ccatttaaca tttggtggat ctcgagaaca tgagatgtca ttaaaacgtc aaggtaagtc
    35161 ttaccttgaa atattagaaa tgggtggtgg catattatct accgttaatg ctactagaga
    35221 aacatccgaa gatgacttgt ttaaaaaagc agagcatgat ttgcttacta tgattaaaca
    35281 cggcgtgctt gcagttgaaa gtaagagtgg ttatggatta gatagagaaa atgaactgaa
    35341 gcaattgaaa gtgtctaatc gtttagctga gaaatatgat ttagacatga aacatacttt
    35401 cctagggcct catgctgtac ctaaagaggc aagttcaaat gaggcatttt tagaagaaat
    35461 gattgcgtta cttccggaag taaaacaata tgcagacttt gcggatattt tctgtgaaac
    35521 aggtgtattt acaatagaac aatcgcaaca ttatatgcaa aaagccaaag aagcaggttt
    35581 taaagtgaaa atacatgcgg atgaaattga tccgttaggc ggactggaat tagcaattga
    35641 tgaacaagca atatcagcag accacttagt agcttctagt gataaaggaa aagaaaagtt
    35701 gcgaaatagt gatactgtag ctgtcctctt acctgcaacg acgttctatt taggtaaaga
    35761 agattatgca gatgcaagag gcatgctaga caataacggt gcgattgcat tagcaactga
    35821 ttataaccct ggtagtagtg tcacaaacaa cttacaactt gttatggcga ttgcagcctt
    35881 gaaattaaag ctatcaccta atgaagtttg gaatgctgtt acggtcaatg ctgctaaagc
    35941 aatagacatt aacgcgggta caattaacac aggtgataag gctaatttag taatttggga
    36001 tgcaccaaat catgaatata ttccatatca ttttggtatc aatcacgcag aaaaagttat
    36061 taaagacggt aaggtcatcg tagataacac attatctttt aaagcataaa ataattagaa
    36121 agctctaagc attggtttac actaatgttt agagcttttg tggtttgtaa aaacaagttt
    36181 atccgagaac acgtttttgt atagattctt aattataaaa gttagaatag tttgacaaca
    36241 gtgttacgat attattaaac aactgtctat cattagcgaa agtaattaac aagagtacat
    36301 tgttgtgaaa tcatcgttta atagtcaaag atgatcatcg tagggtagac aacatatcat
    36361 tgacttaagg aaggggaatg accgatggga ttaaaagatc aagctgttac ctggagaagg
    36421 tattttcatc aatttccaga actatccgat aaagaattta agactacaca aaaaattaaa
    36481 gatattttaa cagaacatca tattagaata ttagacttac cacttgcgac tgggcttgtt
    36541 gctgaagtgg gacaaggtct gagttgtata gctgttcggg ctgacattga tgctttaccg
    36601 attcaagagc ttgttgaaca ggattttaaa tctgaaaatg aaggtgttat gcatgcgtgc
    36661 ggtcatgata ttcacatggc tagtattttg gctacggctg taaaattaaa agaaattgag
    36721 ggcactctta ctgggcgtgt taaattcatt tttcaaccag cagaagaact aggacatggc
    36781 gcttttaaaa ttattgaaac acatgcatta aaagatgtac aagcagtatt gggttttcat
    36841 aatgacccat cgcgttcggt aggtacattt gcaatcaaaa caggggcaat tacatcagca
    36901 gtagatcgtt ttgagtttca tattaaaggc gtgggtggtc atgctgcaaa gccagaacaa
    36961 tgcaacgatc cagttattgt gttggcgcaa ttgattaata gtattcaatc catagttagt
    37021 agaaatctat ctgcgtttga tgaagcggta gtaacaattg gacaaatatc atgtggtaat
    37081 acttggaatg tcattgctga tcatgcgtat gtacaaggca cagtgcgtag ttttgatcct
    37141 gtggtacgaa agcttgtaga aacacgatta caagatattg cggacggatt ggctcaagca
    37201 tataatatga aaattaattt aaattataca catttacctg gcgctgtgat gaatgatgag
    37261 gcattgacgc ataaagcaat tgcagtagcc caacatgttg gatataaggt ggaaatgatg
    37321 gaacagccgc ttacgattgg agaagatttt tcaggttata gtcaacattt tccaagtgta
    37381 tttgcgttaa ttggttctca tagtgaatat gatttacatc atcctcaata taaaccggat
    37441 gaaaggattc ttgaaaaagt acctgagtat tttgttgaat ttgtaaaaag actattgcat
    37501 gagtaattcg agcttgtttt tataaagaag aaagtaattt ttagtgtaat catgattaag
    37561 ttcctaggaa gattgggtaa ataataacca ttaagcgaat aaggagatgt taaaaaatgg
    37621 cagctcaaga tcctagaaca aaatttaaaa caacggatta tgaaaaacaa gaacaagagg
    37681 taccgggttt acaatctgaa atgacgccag cacctgattg tggcgaaacg tcttaccaag
    37741 gtcatcaacg cttacaaggc tataaaatat tagtcacagg tggtgactca gcaatagggc
    37801 gtgcagcagc aattgcgtat gctaaagaag gtgcagatgt agcgattaac tatcttccga
    37861 gtgaagaaca agatgcacaa gaagtacgcc aagtgattga agaaagtggt caaaaggcag
    37921 tgttaattcc tggtgatata agagatgagc agttcaacta tgaccttgtt gaacaagcat
    37981 atcaacaatt aggtggctta gataatgtta ctttagttgc tggacatcaa cagtatcatg
    38041 atgatattca tggatttaca actgaggcat ttacagaaac atttgaaacg aatgtctatc
    38101 cgctattttg gacagttcaa aaagcattgg aatatttaaa accgggtgcg tcaattacaa
    38161 cgacatcatc tgtacaaggt tatcatccta gtcctattct tcatgattat gccgcttcaa
    38221 aagcagcaat tatttcatta actaaaagtt tttcagaaga attaggacca aaaggaatta
    38281 gagtgaactg tgtagcgcct ggtccattct ggtcaccatt acaaatttct ggtggacaac
    38341 ctcaaagtaa aatcccaaca tttggtcaaa aaacaccttt aggtcgtgca ggtcaacctg
    38401 ttgagttatg tggaacttat gtgctattag cgtcagaaga atcaagttat acaactggac
    38461 aagtgttcgg tgttagcggt ggagtgcaaa tagattaggt ttgtatgaag cttttaattg
    38521 aacaacataa tattaattta gttacttcaa tccattaaat taaaatgaat tggtgaagtg
    38581 tattttaaat agtcggtaaa ggtgtgtatc tttatcggct tattttaata ctctgtcata
    38641 aggagcgttt gctcagggtg tggattataa ggtttaagca gaataaactt tcttaatgag
    38701 gatgtcagga caacaacgaa aaaattaaaa aatgttgcgg gattgggttt agggacggtt
    38761 agtggaagaa agttaaattc attaacaagt gaaataccta aggctagata ttaaacgtca
    38821 gaacggacaa agaataatag gatatgtttt caattaaata gcgaagtatt tatatgttaa
    38881 gatgatgtaa attacataga aacgctaccc gtagaagtac tgatcataga aacgctgata
    38941 aacgtaaatt tgcaaactcg atattaaaca aaggggttgt tattatggca ttaatagtat
    39001 ctagagctat attatttttg attttagcat tagcttttgg aatagcgact gtttttcctg
    39061 aacaaaaatt accattatat atagtattca cattatttgt agcagttgcg atcgtttcga
    39121 atgtatggtt tacacatcaa aacgttaaaa agcatatcat taagtttaac aaatacgtcg
    39181 catacttttt atcctttgtc tcagggttac tttacattgg atttgtaatt tatacattaa
    39241 ctatagggag ctatgatgtt ttaccaatta ttatatttgc aataagtatc gtattggttc
    39301 aaatattaga aaatatactg ggaatcagta taaaaaatac gcgtctatca aacaaacagt
    39361 tgaaagtcat ctccaagcaa cgtgttcagc aatttaaagt catatcatta atattagtaa
    39421 gtgtgtctat cgtgttcatt tttattgcat taatgcttaa atggtggctt ttacttgtta
    39481 ttagcattat tgttttgatt attattacag tagcaatgat tgtttatgaa aaatattata
    39541 cgactcctga ttaatttatg attgacgtac taaggattat cttgtaacgt aaacgtaaat
    39601 aagcatcact tatctagaga ggtggaggga ctggccctat gaagcctcgg caacatctcg
    39661 aatgtgccaa ttccagtaac cgtaatggtt tgaagataag caggtaaagc acatgaaaac
    39721 ctctttcttc atcgtttgtg agaaagaggt atttttaatt ggaaagcagg taaaaaggat
    39781 ggaagtacat aaaaagagca atgcttgggc attattcccc ttgttattat ttgtggcgtt
    39841 gtttttaggc gtaggtatta tcacaggtga ttttacttca atgccattaa atgttgcaat
    39901 tacgataacg gtaattgtgg cgttattaat gaatcgaaaa gaatcatttg ctaaaaaagt
    39961 tgaggtcttc acgaaaggtg caggccattc aaacattatt ttaatgatgt tgatttttat
    40021 tttagcaggt gcattttcaa atacagctga aaaaatgggt ggcgtaaagt cgacagttaa
    40081 tttaggacta tcattaatcc cagaaaattt aattattgta ggattgtttg tgatttgtat
    40141 gtttgtttct atatctatgg ggacgtctgt agggactgtt gctgcaatag cacctgttgg
    40201 ttacggattt gcacaagcga cagatgtacc aactgcgcta gcaatggcta cagttgttgg
    40261 aggtgcaatg ttcggcgata atctatcaat gatttcagat acaacaattg ctgcggttag
    40321 aacacaacat acgaaaatga aagataaatt cagagtaaac tttaaaattg tattgccagg
    40381 tgctatttta acgattatta ttctgtattt cttaacgaat ggcatttctt taaatcatgc
    40441 taaaagctat gattataatc taattaaagt tgtaccgtat attttagtat tggtattagc
    40501 attattaggt gtaaatgtca ttatcgtatt gattggcgga acattattag ccggaatcat
    40561 tggtctcata gatggttcat tcggttggat ggggctacta gatgcagtgt ccaaaggtat
    40621 tataagcatg gaagatattg ctatgattgc gttgctaatt ggtggtttag tcggtattat
    40681 tcaacacaat ggtggtattg aatggttgtt gcaatttgtt cgctctaaag tgaaatcaaa
    40741 gcgtggcgca gaattaggaa ttgcgagttt ggtaagtgtc gcagatattg ccacggcaaa
    40801 caacaccatc tctattatta tgtcaggtcc tttagctaaa aatattgcag atgaatatga
    40861 tgtagattca aggaaatcag caagtattct tgatatattt ggaggctgct tccaaggttt
    40921 attaccatat agcccgcaag tcatttcagc ggctggtgta gctggtattt caccgttaat
    40981 gttattccca tacagcattt atcctatatt attaggtgta tgcggactta ttgcgatttt
    41041 atttaatttt ccaaagctaa aaaagaacac tagtcaagaa atgaaacatt aatgcttaac
    41101 aatatatcga tgtagccaag taaggtggct tgttatatta acagatcatg ttgtactcac
    41161 cgtataacgc taatctttta aaaatgatga tcatctatgt ttggaactga gcaaaaatac
    41221 gttaaaataa aagtaagagc gaaagtgggt ggacaacagt ggcaaaattt aatgtagaga
    41281 atgaacatgt cgaagttgaa gttgaaaagc tttataaatt ttctccagag ttagtatacg
    41341 aagcttggac aaagaaagat ttgttgaaac aatggtttat gacatcggcc agaacgaata
    41401 aagagattga agcggacgtt aaagaaggcg gtaaatatcg tattgtagat caacaacgta
    41461 atggcaaagt aaatgtgatt gaaggcattt atgaatcttt agttatggat gaatacgtta
    41521 agatgactat cggtatgcct ggactaagtg aaacacaaga tgtcattgag gtagaatttt
    41581 ttgaacgtga aacaggtggc acccaaatgc tattttatta tcgttcatta gttgaaaaag
    41641 aaagacgatt tactaattta gaatacaagc aaaagaaaaa agaatatcat gatgccatgg
    41701 tgcatggttt cgaattgatg tttgataaga tgtaccacgt tatcgaaaca tcaacacaac
    41761 aataaaatga taaaaggaag ttatgatagg ctcaaggcca ttaaatcata acttcctttt
    41821 ttctaatttg aaatatcatt atgtagtaaa tactcattat attttcttac aagtgtttca
    41881 aagaatatat gataactgat gaagctagaa tagtctgtac catcagttaa gtagaatgtg
    41941 ttactggata catagacgtt gtaatttgct ttttgagcaa gggtattatc tttcattgta
    42001 gtaacagaaa taaagtattt ttgtctaagt tgaagcaagt ttgttacttc taataattca
    42061 tgtgtttcac ctgataatga aataataaag aataaatcat taggttcact cttgttcaaa
    42121 atcattttaa gttcatgttc atcatgaagt acaatgacat gattatgtaa cgttaacaat
    42181 atacgctgtg cctctgaagc aacgtttaac tgtgcacgcc ctgtaccata caaatatatt
    42241 gttgaagctg tatgcatttt ttcagtaaga agctggtaat caacgcgttc taaataactg
    42301 aatgtactat cgatttcttg tttgaaacta tcgatagagt tagaaggtaa ttggtgtgtc
    42361 ttattacttt caaatttaag gtacgattta aaatcactat aaccgtcaaa acctaatttg
    42421 cgtgtaaatc tgtgaatggt tgcattcgag gcatgtgtga attgcgctaa atcttgtatt
    42481 ttcatatttt tacattcatc tatatgtgta ttgataaagt gagcgatatg taaatcatta
    42541 tcattaagtt tatcaaagtt tcgattaatg tgttcatcta aaaacatatt caacctcctt
    42601 gaaaataaat gtgtgataga tcgttattac attaaaatct tgtatgtcat tatatatatt
    42661 tgtgtattga tataggtttg attatgcgtt taaaacgaat gtattacaaa tacaacgatg
    42721 tcatatataa ggtcgtgaaa aaattttcac tatatgaaaa gataatctga ttatacaaaa
    42781 tactttcaaa atacgtcaat gtcgttgtag gtgtgtaagc gtttactata tattatattt
    42841 aaatagtaag acatctattt ataaatgaat ctaattagat gtattgttag gaaagatgat
    42901 atggagggaa tagaagatga atgcgataaa gcgtttcggt agtgcaatga ttgtaccggt
    42961 attaatgttc gctttctttg ggatagtgct tggctttgcg acattattta aaaatcctac
    43021 aatcatgggg agtttagcag atcaacatac gttttggttt aaattttggt cagtaataga
    43081 atctggtggc tgggttattt ttacgcatat ggaagttgta tttgttgtag gtttaccact
    43141 ttctttagct aaaaaagcac caggtcatgc agcacttgca gcattgatgg gttacttaat
    43201 gtttaataca tttataaatg ccattttaac gcaatggcca catacgtttg gcgctaattt
    43261 agaaaaaggc gttgaaaatg tacctggttt aaaatcaatt gcaggtattg ccacattgga
    43321 tacgaatatt ctaggtggta ttattatttc agctattatt acttggattc acaatagata
    43381 ctatagtaaa cgattaccag aaatggttgg tgtattccaa ggtttaacat ttgtagttac
    43441 gatttcattt tttgtgatgt taccattagc agcaattact tgtgttattt ggccaactgt
    43501 tcagcacggt attggttcaa tgcaacactt tatcatcgct tcaggctata tcggtgtttg
    43561 gctgtaccac ttcttagaac gtgtattaat accaacagga ttacatcact ttatttatgc
    43621 accaattgaa gttggaccag tagtagtcaa tcacggcttg aaagctgaat ggttacaaca
    43681 tttaaatgag tttgctaaga gtactaagcc attaaaagag caattccctt atggctttat
    43741 gcttcaagga aatgggaaag tatttggatg tttaggtatt gctttagcaa tgtatgctac
    43801 gacacctaaa gaaaatcgta aaaaagtagc agcattactt attcctgcaa cattaacagc
    43861 agtcgttgta ggtattactg agccattaga atttacattc ttattcatcg caccatactt
    43921 atttgtttta cacgcagttt tagcagcatc aatggatact ttaatgtacg catttggtgt
    43981 tgtaggtaac atgggtggtg gcttgttaga ctttatttca acaaactggt tgccattagg
    44041 taaagaacat tggggtactt atgttgcaca agttattatc ggtctgatat ttgtagcgat
    44101 ttacttcttc ttattcagat tcttaatttt gaaatttgat attccattac ctggacgtaa
    44161 gaaaacagaa gaggaagtta aattattttc taagcaagat tacaaaaata aaaaaggtga
    44221 tagcgtagat tctaaacgtg cttcaacagg taatgaatac gaagacaaag ctgcgtatta
    44281 cctcgacggt ttaggcggca aagaaaatat caaagatgtt actaactgta caacaagatt
    44341 acgcttaact gtttatgatg aaagtaaagt tgcggatacg gaatatttca cgcatcaaca
    44401 aatggcacac ggattagtaa agagtggtaa gagtatccaa gttgtagtag gtatgacagt
    44461 accacaagtt cgtgaagcat ttgaacaaat ggttgaggat caatcgtccg aagataaata
    44521 atacaaaaaa agacacatca ctatacgcaa tcattgtaaa tgatcatcgt atagatgtgt
    44581 cttttgttat gatatacgag ctttgctcat agatgacgat ctataattgg cagatagaat
    44641 gtcgatattt ctagttgcaa tcgtatgtaa caatttagca aatatttctg gcgtacatat
    44701 aatatgtgca tgagacttat tcaacgtata agtgatatca gtgtgatttt ggaacatatt
    44761 cataatatcg atgactttgt ctttaatgaa agcatcttgc aagccatcaa tattgatatc
    44821 gaaacgcttt gcagattgat ataaattatc agaatcaagt aattcattta ttttctttgg
    44881 tgaaagtact tggaaaatac tatactgatg aaaacttaaa aatgaaatga agtatggaag
    44941 ttgttgcttt ggtaagctaa tttctaatac atactgatta aactgtttgt ttattgagaa
    45001 ttgcaacgca tcacaaaatt gtacactttt gcataactta cttaattttt ttctagtagt
    45061 acggtcattt aaaccggcga tgttgatgat gtatgtttcg aatttatcaa ttaacatgtg
    45121 aggacctccc gaggaataca tggcattaaa tacacgttta atatttataa aggtgactta
    45181 attttgttca agttgatttt accacgcttt ttttctttat tcactaagac ttttgaatga
    45241 agtttaaaat aattttttat cagtgataaa atatttgcaa taagaagaga atgactaaat
    45301 aatcttaatt ttcagaaaag taattgtaac cttactggtc ttatggtaat atttttcaat
    45361 attatcgacg aggatgtgtt aacaatgttt caaaaattga gtatatttgc cacgaagagt
    45421 tttttggtat ggatgttagt agcagctgtt attggattta tttccccaca acatgttgcg
    45481 acattaggta agtgggtacc ttatttactt ggtattgtta tgttaggtat gggattaaca
    45541 attacaccta atgatttcaa aatggtcttt aaagcaccta gagcagtaat tattggtgtc
    45601 tgtctacaat tcagtattat gcccacatta gcatttataa ttgcaaagtc ttttcattta
    45661 ccacctgata ttgctgttgg cgtaatatta gttggatgtt gtccgggtgg gacatcaagt
    45721 aatgtaatga gttatttagc caaagctaac gtagcacttt ctgtttctat tacgacggtc
    45781 tctacgttgc tagcgccatt cgttacacct gcgttaatat atctatttgc aaatgaatgg
    45841 ttggaagtat ctttcttgag tatgttgtgg tcagttgttc aagttgtatt aattccaatt
    45901 gctttaggta ttgttttgca aattattaat cgtaaaattg ctgaaaaagc ttctacagct
    45961 ttgccaatta tatcagttgt tgctatttca ttaattttag caatagttgt aggtggcagt
    46021 aagcaccaaa tcttaactac aggattatta atatttttag tagttatttt acataacgta
    46081 ttaggatata cgattggata ttggttagct cgtcttttaa aattagatcg acaagatcaa
    46141 aaagcagtca gtattgaagt tggaatgcag aactctggtt tagctgtgtc attagcagca
    46201 ttgcatttta atccaattgc agcagtacca ggcgcagtgt ttagtttcat tcataatata
    46261 acagggccta ttttagcaaa gtattggtca aaaaagttat aattgcacta atagaatgaa
    46321 gtggtcatcg gactgtgtta agctttgata aagagaaaaa atagaggagt aaatatatgt
    46381 atagagcagt tatatttgat ttcgatggaa caataataga tacggaacaa catttattta
    46441 atgttattaa taaacattta gagatgcata atgccgatcc tataagcatt gatttttatc
    46501 gttcttctat tggaggagca gctacagatt tgcatgacca tttaattaaa gcgattggtt
    46561 cggaaaataa agataaactt tatgaagaac atcatcttac tagtacaaca ttaccgatga
    46621 ttgatacgat taaatcattg atggcatttt taaagcaacg tcacattcct atggcgattg
    46681 ccacaagtag tgtgaaagcg gaaataatgc ctacctttaa agcattaggt ctagatgatt
    46741 atatagaggt agttgttggt agagaagatg ttgaacaagt taaacctgat cctgaattat
    46801 atttatctgc agtacaacaa ttaaattata tgccgacaca atgtttggct attgaagatt
    46861 ctgtaaatgg tgcaacagcc gcgattgcag ctggattaga tgttattgtt aatacgaata
    46921 aaatgacaag cgcacaggac ttttctaatg tagattatgt agcaaaagat attgattacg
    46981 atcaaattgt agcgcgtttc tttacgaaat aggaggcgta tcatgatggg ttacattata
    47041 ttgttttttc tagctggtcc agtaatttta ggcgttggaa atttggtgat tggtcctata
    47101 tttaacaaac agacaccatt tcgcgtgcaa gtaagatctt ttgttgttgg ttcaatgatt
    47161 tacttaatac tcgcaacaat tggctatttt ttactattac aaggtaaact ttaacgagaa
    47221 aaccacctta cctcattaaa tggacgacca tatgtatgtg aaatggtaga acgttcatgt
    47281 ttatgtatga gatagggtgg tttaaatagt tacatatatt ttaataataa cgtcacgatg
    47341 ataagtacaa ttaagataat atctatgcct actataattg tagctcttgt tgcattactt
    47401 ccttgttctt ttgctgattt catagcacgg tagtttggca caaagctaat aattagtaag
    47461 attaatacaa ttacaccaat taatgctgtt gtcatgatga acgacctcct ttattttttt
    47521 caatcaattc ccaaataaac gtagcaatca caccgatgat taacccagtg atgacaccga
    47581 tttgtaccgt taaaaatatg gcgccgaaaa tgataccaat aattaatgca atcggtaaag
    47641 tcgtaccgag tttaatcttg cgctctggag aattaataat agtaaatact gtaagacaaa
    47701 tgagtatgaa agcaagtgtt gcaataatag ttcttccaac taaatatagg atgtcaggtt
    47761 tttccatacc gatataattt atgatgaaaa atgctacagc aaagagtacc gatatttttg
    47821 tagcacgtag cagtatttgt tttaacattg atatactcct ttttaatatt attaaaatta
    47881 tatcataatt accaagagta gctgaagttg tatgtgactc aacggttcac ggacaacttt
    47941 tttaattttt tagaaaaatc acaaaataat tgtttgcaaa gttgcaaaag cctgctatag
    48001 tagttctgta aacgattgca tggtatgcaa atattaatgt accaaaatcg ataatttata
    48061 gtataattac ggcaataagt ttttttatgg atttatttag tatcaatcag agatggggta
    48121 agaagttatg gagaacaatg aactacaaag gggattgagt gcccgtcaaa ttcaaatgat
    48181 tgcacttggt ggtacgattg gcgtggggct tttcatgggt gcgacaagta caattaaatg
    48241 gacaggccca tcagttatcc ttgcatattt aattgcgggt atctttttat ttttaatcat
    48301 gagagcaatg ggggaaatga tttatttaaa ccctacaaca ggatcatttg caacatttgc
    48361 aagtgattat atacatcctg cagcaggtta tatgacagca tggagtaata tattccaatg
    48421 gattgtagtt ggtatgagtg aggtcatcgc agtaggagaa tatatgaagt tttggttccc
    48481 ggaattgcca acttggattc ctggtgttat tgctatttta ttattaatgg cagcgaattt
    48541 attctcggta aaagcgtttg gagaatttga attttggttt gctttaatta aagttgtaac
    48601 aattatttta atgattattg ctggttttgg tcttattttc tttggttttg gaaatggtgg
    48661 ccatgcggta ggtatttcta atctatggac aaatggcgga tttatgccaa atggtattgt
    48721 tggtttcttc tttgcattat caattgtaat tggttcatac caaggtgtgg aactgatagg
    48781 tattacggca ggtgaaacta aagatcctca gaaaaatatc gttaaagcag tgaatggtgt
    48841 tatctggaga attttaattt tctatttagg tgctattttt gttattgttt cagtgtatcc
    48901 ttggaatcaa ttaggagaca ttggaagtcc gtttgttgca acatttgcta aaatcggtat
    48961 tacatttgca gctggattaa ttaactttgt tgtattaact gcagcaatgt caggatgtaa
    49021 ctcaggtatt ttcagtgcga gtcgtatgat ttatacactt gctcataaag gtgaaatgcc
    49081 gaaaatattt actaaaataa tgagaaatgg tgtaccattg tacactgtta tagcagtatc
    49141 tcttggtatt ttaattggtg ctttattaaa cgtaatttta cctttatata tcgatggagc
    49201 gaagagtatt ttcgtatacg tttatagtgc gtctatttta ccaggtatga ttccttggtt
    49261 tatgatttta ttcagtcact tacgttttag aaaattacac cctgaagaat tggaaggtca
    49321 cccattcaaa atgcctggtg gcgcagtaac taactattta acaatcttat tcttaatatt
    49381 agtattggtt ggtatggtat ttaatgttga aactagaata tcagtgctaa ttggtgtcat
    49441 cttcttaacg attgtaacga tttattactt tattagatat aataaaaata atgtaaaagc
    49501 aaaatagtat gattataatg tgcgcttatt ttatgacaaa tttggcccta gcttattaaa
    49561 gttaaatagc caaagaatgt tatgagtagg cgcatttttt tataaatata gcttaaatcg
    49621 aagttgtagt gtgaaaaact tataagtggc aatattatcg ttagttttta ctgtgggctt
    49681 taaaataaaa aatagcatta ttaaaataac aaacgcaatt gatatatgga atgaatccgt
    49741 taatcaactg cgtttgtttt taatataaga aattgaatgg ccttatcaaa tcattggtca
    49801 tatgcgtatg ttatacaaga taaaagacat gtaagctttg aaaatgatgc ataaattatt
    49861 atatgacatg attaaagaat ttatataaga ctgttaatgt ttaaagttaa tatttgataa
    49921 atgtttacga tagttatcta aagccatttt agattgtgtt atgaaatcta atgatgcgtg
    49981 ataatttaat gcaacataac gataatataa aatatcaata gtgaacattt gagcaaataa
    50041 tgaagttgtt gctcccatat gcatctcatt ttcatcagtt ttcccataag ttaaaacaat
    50101 atttgatgcc tgtgctacgg gattatccct tgtactagta attgtaatta taggtatatg
    50161 gtagtcatca ataactttaa ccattgattg catttcactt tgcgtaccat tgttagtaat
    50221 aagaataaca ctgtcgttcg aattgtgagt tgctaataat gttgcaaaaa tatgtgtttc
    50281 ttgaacaagt tgaatattaa gacctattct tgataacttt tggtataagt cggtagcaac
    50341 tacaaaagat gcgccaaaac catatataaa aatcgtctca gaacgtttta aacaatgaca
    50401 tatttgatca atagttttat catttaattc gttattagca tgattaagtg cgcgtgtagt
    50461 acgtgtatgg agtttagttc ttaaagattc tgtgctttcg ttattcatta attcaacatt
    50521 gtaaattgat gatgctttag gaacatattt agatatattt attttcaagt cgtgaaaacc
    50581 gccatcagta attttcttgc tgaatcgaat gactgatgat gtactaatat ctaataagct
    50641 tgctaagtct tgagacctca ttttaatcac cttttgaggt gaatttagta taaatttagc
    50701 tatctttttt tcatttttag tcatatatgg atattgacta tctatttctg ttagtacgtt
    50761 tgacatatta atcactccac tttaacgcaa tatattttat tagcgttggc tattgttgtt
    50821 taacgcgatg gtatttacaa cttgtaacgt aagagaatga aaaataagct gaatatgttg
    50881 tttgtttaat gatattgaat tataaaactg taaatatttt taagatgtat agataaaaaa
    50941 tagcatactc ccatgtgcaa ataatatatc aatatattga tatattaccg taagtcatac
    51001 tgtatattcc caatttatac attatgtact catctattta aatctta
//
LOCUS       DAMZLE010000006        49563 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMZLE010000006 DAMZLE010000000
VERSION     DAMZLE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 49563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.006"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..595
                     /locus_tag="POB92_000336"
     CDS             71..595
                     /locus_tag="POB92_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDI6577862.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            622..1860
                     /locus_tag="POB92_000337"
     CDS             622..1860
                     /locus_tag="POB92_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI6577863.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            1888..2517
                     /gene="upp"
                     /locus_tag="POB92_000338"
     CDS             1888..2517
                     /gene="upp"
                     /locus_tag="POB92_000338"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI6577864.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            2541..3668
                     /gene="wecB"
                     /locus_tag="POB92_000339"
     CDS             2541..3668
                     /gene="wecB"
                     /locus_tag="POB92_000339"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDI6577865.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKLSDF"
     gene            3736..4188
                     /locus_tag="POB92_000340"
     CDS             3736..4188
                     /locus_tag="POB92_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDI6577866.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLLIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            4209..4937
                     /gene="atpB"
                     /locus_tag="POB92_000341"
     CDS             4209..4937
                     /gene="atpB"
                     /locus_tag="POB92_000341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI6577867.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            4980..5192
                     /gene="atpE"
                     /locus_tag="POB92_000342"
     CDS             4980..5192
                     /gene="atpE"
                     /locus_tag="POB92_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDI6577868.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            5389..5910
                     /locus_tag="POB92_000343"
     CDS             5389..5910
                     /locus_tag="POB92_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI6577869.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            5910..6449
                     /locus_tag="POB92_000344"
     CDS             5910..6449
                     /locus_tag="POB92_000344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI6577870.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            6471..7979
                     /locus_tag="POB92_000345"
     CDS             6471..7979
                     /locus_tag="POB92_000345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI6577871.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            8010..8876
                     /locus_tag="POB92_000346"
     CDS             8010..8876
                     /locus_tag="POB92_000346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI6577872.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            8898..10310
                     /gene="atpD"
                     /locus_tag="POB92_000347"
     CDS             8898..10310
                     /gene="atpD"
                     /locus_tag="POB92_000347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI6577873.1"
                     /translation="MGIGRVTQVMGPVIDVRFKHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            10330..10734
                     /locus_tag="POB92_000348"
     CDS             10330..10734
                     /locus_tag="POB92_000348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI6577874.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            11072..11164
                     /locus_tag="POB92_000349"
     CDS             11072..11164
                     /locus_tag="POB92_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577875.1"
                     /translation="MLKSKFHSPLSTVDISFFESSYIFSYQDTL"
     gene            11296..11529
                     /locus_tag="POB92_000350"
     CDS             11296..11529
                     /locus_tag="POB92_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDI6577876.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            11640..12905
                     /gene="murA"
                     /locus_tag="POB92_000351"
     CDS             11640..12905
                     /gene="murA"
                     /locus_tag="POB92_000351"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6577877.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            12939..13379
                     /gene="fabZ"
                     /locus_tag="POB92_000352"
     CDS             12939..13379
                     /gene="fabZ"
                     /locus_tag="POB92_000352"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI6577878.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(13437..13877)
                     /locus_tag="POB92_000353"
     CDS             complement(13437..13877)
                     /locus_tag="POB92_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDI6577879.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            14071..14466
                     /locus_tag="POB92_000354"
     CDS             14071..14466
                     /locus_tag="POB92_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6577880.1"
                     /translation="MLNKIVIVERLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            14855..15550
                     /gene="sceD"
                     /locus_tag="POB92_000355"
     CDS             14855..15550
                     /gene="sceD"
                     /locus_tag="POB92_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDI6577881.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      15892..15994
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16074..16763
                     /gene="tenA"
                     /locus_tag="POB92_000356"
     CDS             16074..16763
                     /gene="tenA"
                     /locus_tag="POB92_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDI6577882.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            16756..17586
                     /gene="thiD"
                     /locus_tag="POB92_000357"
     CDS             16756..17586
                     /gene="thiD"
                     /locus_tag="POB92_000357"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6577883.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRRLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            17570..18370
                     /gene="thiM"
                     /locus_tag="POB92_000358"
     CDS             17570..18370
                     /gene="thiM"
                     /locus_tag="POB92_000358"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDI6577884.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQGLLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVEKYV"
     gene            18363..19004
                     /locus_tag="POB92_000359"
     CDS             18363..19004
                     /locus_tag="POB92_000359"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDI6577885.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIENTVNRFKDFFNN"
     gene            19090..19962
                     /gene="yidC"
                     /locus_tag="POB92_000360"
     CDS             19090..19962
                     /gene="yidC"
                     /locus_tag="POB92_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI6577886.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(20576..21223)
                     /locus_tag="POB92_000361"
     CDS             complement(20576..21223)
                     /locus_tag="POB92_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6577887.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKLYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(21251..22735)
                     /gene="cls"
                     /locus_tag="POB92_000362"
     CDS             complement(21251..22735)
                     /gene="cls"
                     /locus_tag="POB92_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6577888.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            22901..23194
                     /gene="csoR"
                     /locus_tag="POB92_000363"
     CDS             22901..23194
                     /gene="csoR"
                     /locus_tag="POB92_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDI6577889.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            23206..23415
                     /locus_tag="POB92_000364"
     CDS             23206..23415
                     /locus_tag="POB92_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDI6577890.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVI"
     gene            23543..23680
                     /locus_tag="POB92_000365"
     CDS             23543..23680
                     /locus_tag="POB92_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577891.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(23943..25145)
                     /locus_tag="POB92_000366"
     CDS             complement(23943..25145)
                     /locus_tag="POB92_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDI6577892.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            25442..26512
                     /locus_tag="POB92_000367"
     CDS             25442..26512
                     /locus_tag="POB92_000367"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6577893.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIISTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            26527..27885
                     /locus_tag="POB92_000368"
     CDS             26527..27885
                     /locus_tag="POB92_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI6577894.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQVINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            28402..29922
                     /locus_tag="POB92_000369"
     CDS             28402..29922
                     /locus_tag="POB92_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6577895.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(30291..30986)
                     /locus_tag="POB92_000370"
     CDS             complement(30291..30986)
                     /locus_tag="POB92_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6577896.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(30986..33643)
                     /locus_tag="POB92_000371"
     CDS             complement(30986..33643)
                     /locus_tag="POB92_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDI6577897.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGIHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKVEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDV"
     gene            33781..33891
                     /gene="kdpF"
                     /locus_tag="POB92_000372"
     CDS             33781..33891
                     /gene="kdpF"
                     /locus_tag="POB92_000372"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDI6577898.1"
                     /translation="MLLCCKEVMTMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            33915..35591
                     /gene="kdpA"
                     /locus_tag="POB92_000373"
     CDS             33915..35591
                     /gene="kdpA"
                     /locus_tag="POB92_000373"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDI6577899.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVFIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            35610..37637
                     /gene="kdpB"
                     /locus_tag="POB92_000374"
     CDS             35610..37637
                     /gene="kdpB"
                     /locus_tag="POB92_000374"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDI6577900.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKTFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            37657..38217
                     /locus_tag="POB92_000375"
     CDS             37657..38217
                     /locus_tag="POB92_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDI6577901.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            38426..38905
                     /locus_tag="POB92_000376"
     CDS             38426..38905
                     /locus_tag="POB92_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577902.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLCIKPLWQYHCRFYKVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            38898..40481
                     /locus_tag="POB92_000377"
     CDS             38898..40481
                     /locus_tag="POB92_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6577903.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            40468..40959
                     /locus_tag="POB92_000378"
     CDS             40468..40959
                     /locus_tag="POB92_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6577904.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNNISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            40963..41322
                     /locus_tag="POB92_000379"
     CDS             40963..41322
                     /locus_tag="POB92_000379"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI6577905.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            41388..42536
                     /gene="alr"
                     /locus_tag="POB92_000380"
     CDS             41388..42536
                     /gene="alr"
                     /locus_tag="POB92_000380"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6577906.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            42621..42791
                     /gene="mazE"
                     /locus_tag="POB92_000381"
     CDS             42621..42791
                     /gene="mazE"
                     /locus_tag="POB92_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDI6577907.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            42788..43150
                     /locus_tag="POB92_000382"
     CDS             42788..43150
                     /locus_tag="POB92_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6577908.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            43499..44500
                     /locus_tag="POB92_000383"
     CDS             43499..44500
                     /locus_tag="POB92_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDI6577909.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            44619..44945
                     /locus_tag="POB92_000384"
     CDS             44619..44945
                     /locus_tag="POB92_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDI6577910.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            44947..45426
                     /gene="rsbW"
                     /locus_tag="POB92_000385"
     CDS             44947..45426
                     /gene="rsbW"
                     /locus_tag="POB92_000385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDI6577911.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKNENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            45401..46171
                     /gene="sigB"
                     /locus_tag="POB92_000386"
     CDS             45401..46171
                     /gene="sigB"
                     /locus_tag="POB92_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDI6577912.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            46604..48754
                     /locus_tag="POB92_000387"
     CDS             46604..48754
                     /locus_tag="POB92_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI6577913.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKNDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            48747..49202
                     /locus_tag="POB92_000388"
     CDS             48747..49202
                     /locus_tag="POB92_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDI6577914.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT        17489 a         7197 c         9338 g        15539 t
ORIGIN      
        1 aataagttat aatagaggag tgtagcactt tgctatattc cttttttatt tttaggaagg
       61 gtgggctttt atgaaagatt tgacaatgtt attagacgaa ttaaaagata tgtccttttt
      121 taataaagga gatatatgtt taattggatg ttcaacatcg gaagtcattg gcgaaaaaat
      181 agggactgtc ggttcgatgg aagttgcaga aactattttt aatgcattag atgttgtgag
      241 taaagagaca ggcgttactt ttgcttttca aggatgtgaa catatcaaca gagcgataac
      301 aatagaaaaa tctcagtata atccattaac aatggaagaa gtttccgtcg ttccagatgt
      361 acatgctgga gggagtttgg caacatacgc ttttcagcat atgaaggatc cgatcgtggt
      421 cgaacatatt actgttcctt gtggaattga tattggtcaa acgttaatag gcatgcatat
      481 taaacatgta tgtgtacctg taaggacctc agtaaagcaa gtaggacagg caattgtaac
      541 tattgccaca tctaggccga aaaagattgg tggcgaacgt gctaaatacc aataagtttt
      601 tttgaaagaa ggggagattt tatgtcttat atcaccaagc aagataaagt tatcgcagaa
      661 gcaatcgaga gagaatttca gagacaaaat agcaacatag agttaatcgc atcggaaaat
      721 tttgtatcgg aagcggttat ggaagcacaa ggttcagtgt tgactaataa gtatgctgaa
      781 ggctatccag gacgccgata ttatggtggc tgtgagtttg tagatgttac tgaaagcatc
      841 gcaattgatc gtgctaaagc attgtttgga gctgaacatg tcaatgttca accacattca
      901 ggttcacaag cgaacatggc tgtttactta gttgcattag aaatgggcga cacagtttta
      961 ggtatgaatt tgagtcatgg tggtcacttg acacatggag cgcctgttaa ttttagtggt
     1021 aaattctaca atttcgttga atatggagta gataaagaca cagaacgaat caattatgat
     1081 gaagttcgta aattagcgtt agagcataag cctaagctta ttgtggcagg agcatcagca
     1141 tattcaagaa caattgactt caaaaagttt aaagaaatcg cagatgaagt aaacgctaag
     1201 ttaatggtag acatggcaca tattgcagga ttagtagcgg caggtttaca tccaaatcca
     1261 gtagaatatg ctgattttgt aacaactaca acacacaaaa cattacgcgg accacgtggt
     1321 ggtatgattt tatgtaagga agaatataaa aaagacatag ataaaacaat tttccctggt
     1381 attcaaggtg gacctcttga gcatgttatt gcagcaaaag cagttgcttt tggagaagcg
     1441 ttagaaaata atttcaaaac gtatcaacaa caagtggtta aaaacgcaaa agttcttgca
     1501 gaagcattaa ttaatgaagg atttagaatt gtttctggcg gtacagataa tcacttagta
     1561 gctgttgatg taaaagggtc tataggactt actggtaaag aagctgaaga gactttagat
     1621 tcagttggta tcacatgtaa caaaaatacc attccgttcg atcaagaaaa accttttgta
     1681 acgagtggta tacgtttagg tacacctgct gcaacaacgc gtggatttga tgaaaaagct
     1741 tttgaggaag ttgcaaaaat catcagttta gcattgaaaa atagtaaaga tgaagaaaaa
     1801 ttacaacaag ctaaagaacg cgttgcgaaa ttaacagctg aatatcctct atatcaataa
     1861 acatacacaa agataggagt aatttttatg agtaaagtac acgttttcga tcatccatta
     1921 attcaacaca aactaagcta tattagagat gtaaatacag gtactaaaga atttagagaa
     1981 cttgtagacg aagttggtat gttgatggca tatgaagtaa ctagagactt agagcttcag
     2041 gatgttgata ttgaaacgcc tgtaactaaa atgacggcta aacgattagc tggtaaaaaa
     2101 ttagctatcg ttccaatttt aagagctggt ttaggtatga ctgatggtat tttaagttta
     2161 gtgcctgcag ccagagttgg acatatcggt ttgtatcgag atcccgaaac attaaaggct
     2221 gtagagtatt ttgctaagtt acctcaagat attactgaaa gacaaatcat tgttgttgat
     2281 cctatgttag caacaggcgc atcagcaatt gaagctatta cttcattgaa gaaacgtggc
     2341 gctaaaaata ttcgcttcat gtgtttaatt gcagcaccag aaggcgtaga aaagatgcat
     2401 gaagcacatc cagatgtaga tatttatatt gctgcacttg atgaaaagct aaatgataaa
     2461 gcatatatca caccagggtt aggcgacgct ggagatagat tgtttggtac taaatagaag
     2521 aatcggagat aagtagtttg atgaaaaaga ttatgaccat atttggaaca agacccgaag
     2581 ccataaagat ggctccttta gttaaagctt tggaacaaga aaaaatgctc gagccaattg
     2641 ttgttgttac agcacagcat agagaaatgc ttgattcagt attaagtacc tttgaaatca
     2701 aaccaaaata cgatttgaat attatgaaat caggacagac actttctgag ataacttcta
     2761 aatcaatcac tcaattagaa caagtgattc aattagaaaa accagacatg gtacttgtac
     2821 atggtgatac gatgacgaca tttgcaggtg gtttagcggc attttataac caagtgccga
     2881 ttggacacgt tgaagcaggg cttagaagtt atgataaata ttcacctttt ccggaagagg
     2941 tgaatagaca attagttggt gtattagctg acttacattt tgcacctact aaaaatgctg
     3001 catcgcattt gttaagtgaa gggaaatatt cagaaagtgt tgttgtcact ggtaatactg
     3061 cgattgatgc tatgaaatat acagttgatg acaattataa atctaacatc atggataaat
     3121 accatgacaa aaaattcatt cttatgacag cacaccgacg agaaaatatc gggaaaccca
     3181 tggaaaatat atttaaagcg gttagacgct taatagatga gtatactgat ttagcactgg
     3241 tatatccaat gcataaaaac ccgaaagttc gagaggtggc tcaaaagatt ttaggtagtc
     3301 atgacagaat tgaattgatt gaacctttgg acgtagtaga tttccataat tttgctaaaa
     3361 aatcttattt tattttgaca gattcaggtg gaattcagga agaagcgcca tcatttaata
     3421 agccagtttt agttttgcga agcgttacag aacgtcctga aggtgttgaa gctggcacat
     3481 taaaagtcat tggaacaaat aagcaaaatg tgtatcaagc tgcaaaagaa ttaatcgatg
     3541 atgagagatt atatcatcaa atgtctgaag catctaatcc ttacggtgat ggatttgctt
     3601 cagaaagaat agttaatcat attaagtatt atttgaattt aatcactgaa aaactaagcg
     3661 atttctgagg aaaatagact ttttgtctaa ttgtgtctaa attgtgtcta actatgctta
     3721 tttttttaca acaatttgaa tagaattgac accacaaatc gcctatatta tcatttagtt
     3781 tgtatgagtg gaacggtttt catgattgat atcagtcaca cggaggcata taaaatgagt
     3841 cgtttctaca acatttttaa acagttcatt caatattatt tttatctatt aataatattg
     3901 ggaggattat acctttatac acaccatgca tttatcttag gattaatcat tggtgttact
     3961 ggttctgcaa tcaacacatg tatttttgaa tgctatttag ctaaagctaa aagaccagac
     4021 actatgcata tttcaactgg aaatatgtgg cgatacttag ttgcaattat tgcctgtatg
     4081 atttggtacc ttaataaagc gcatgtaagt atcatcggta taattattgg tttaatgatt
     4141 tcatatgttg tagttatcat acgtccttta ctaaaggtga gcaaataaat taagaaagag
     4201 gtgagattat ggatcacaaa tccccgctcg tgagttggaa tttattcggt tttgatatcg
     4261 ttttcaattt atcaagtata ttgatgatac ttgttacggc gtttcttgtt tttctacttg
     4321 ctatcatttg tacgcgtaat ttgaaaaaaa gaccaactgg caaacaaaat ttcgttgaat
     4381 ggatttttga tttcgtgagg ggaatcattg aaggtaacat ggcttggaaa aaaggtggtc
     4441 aattccactt cttagcagta acgctgattc tgtacatttt tatagctaat atgttaggtc
     4501 ttccgttttc tatagtaacg aaagatcaca cattgtggtg gaaatcaccg acagctgatg
     4561 caacagtgac tttaacgttg tctacaacga taatactgtt aactcacttt tatggaatta
     4621 aaatgcgtgg tacgaaacaa tatcttaaag gttatgtaca gccgttttgg ccattggcaa
     4681 ttattaatgt tttcgaagag ttcacttcaa cattaacgct tggtctgcgt ttgtacggta
     4741 acatatttgc aggtgagata ctattaacat tacttgctgg cttattcttt aacgaaccag
     4801 catggggttg gattattagt atcccaggat taattgtttg gcaagcattt tcaatatttg
     4861 taggaacaat ccaagcatat atctttatta tgctttcgat ggtttatatg tcacataaag
     4921 tggcagatga acactaaaaa tttcaataat tatatacaat cacaggagga aattaaatta
     4981 tgaatttaat cgcagcagca atcgcaattg gtttatcagc attaggagca ggtatcggta
     5041 acggtttaat cgtttcaaga acagttgaag gtgtagcacg tcaaccagaa gcacgtggtc
     5101 aattaatggg tatcatgttc attggtgtag gtttagttga ggcattacct atcatcggtg
     5161 tagtaattgc attcatgaca tttgctggat aattaacaga taaaagaggt cgggacaaag
     5221 cgcataggac ataattcatg atgcatatat agtaatatct ttgaacttta ttaaatagtt
     5281 gagatatgaa tgcaccatgc ctatcgcata aattcagtag gtcctaacct cgtcgttttt
     5341 tctatataac actagcgatt attttaatga aaggagtgtc atgaacccgt gactgaaaca
     5401 gctaacttat tcgttcttgg tgcagctgga ggcgttgagt ggggtactgt gattgtacag
     5461 gtcctaactt tcatcgtgtt acttgcgtta cttaaaaagt tcgcatgggg tccattgaaa
     5521 gatgtaatgg ataaacgtga aagagatatt aacagagata tcgatgacgc agaacaagct
     5581 aagttaaatg cacagaaact tgaagaagaa aataaacaaa aacttaaaga aacacaagaa
     5641 gaagttcaaa agattttaga agatgctaag gttcaagcac gtcaacagca agaacaaatt
     5701 attcatgaag caaacgtacg tgcaaacggt atgattgaaa cagcacaaag tgaaatcaat
     5761 agccaaaaag aacgtgccat tgcagatatt aataatcaag tatctgaact atcagtgtta
     5821 attgcttcta aagttcttag aaaagaaatt tctgaacaag accaaaaagc attggttgac
     5881 aagtatctaa aagaggcagg cgataaataa tggtaaaagt agctaacaag tatgctaaag
     5941 cattatttga cgtgtcatta gatacaaata atttagagac tattaatgaa gaattaacag
     6001 ttataaatga agcagtaaaa gataaaattg agcaattgaa aatggttgac agtaatccaa
     6061 ctcaaacagc agaacaacgt cgtgagttga ttaatggtgt atttactgac attaatccat
     6121 acattaaaaa tatgatgtac gtgttagcag ataatagaca tatctcatta atagctgacg
     6181 tattcaaggc gttccaaagc ttatataacg gacactacaa tcaagatttt gcaacaattg
     6241 agtcaacata tgaattgagt caagaagagt tagataagat tgtcaaacta gtaactcaac
     6301 aaacgaagtt atctaaagtt attgtagata caaaaattaa tccagattta attggtggat
     6361 ttagagttaa agtcggcaca actgtattag atggtagtgt tagaaatgat cttgtccaat
     6421 tacaaagaaa atttagaaga gttaattaat tataaagagg agtgacatag atggccataa
     6481 aagctgaaga aatcagtgca ttacttcgct cacaaattga aaattatgag tcagaaatgt
     6541 ccgtaactga tgtaggtact gtattacaaa ttggtgatgg tattgcatta attcacggat
     6601 taaatgacgt tatggctggt gagctagtag aattccataa cggcgtactt ggtttagccc
     6661 aaaaccttga agagtcaaac gtgggtgtgg ttattttagg accatacaca ggtattactg
     6721 aaggtgacga agttaaacgt actggtcgta tcatggaagt accagtaggt gaagaactaa
     6781 tcggaagagt tgttaatcca ttaggacaac ctattgatgg acaaggaccg attaacacaa
     6841 ctaaaacacg tccagtagag aaaaaagcta ctggtgtaat ggatcgtaaa tcagtagatg
     6901 agccattaca aacaggtatc aaagcaattg atgctttagt accaattggt agaggtcaac
     6961 gtgagttaat catcggtgac cgtcaaacag gtaaaacaac aattgcaatt gacacaattt
     7021 tgaaccaaaa agatcaaggt acgatttgta tctatgttgc tattggtcaa aaagattcaa
     7081 cagtaagagc aaatgttgaa aagttaagac aagcaggcgc tttagactac actattgttg
     7141 tagcagcatc agcttctgaa ccttctccat tattatatat tgcaccatat tcaggtgtaa
     7201 caatgggtga agaattcatg tttaacggta aacatgtttt aatcgtttat gatgatttaa
     7261 ctaaacaagc agcagcttat cgtgagttat cattattatt acgtagacct ccaggtcgtg
     7321 aagcataccc aggtgacgta ttctacttac atagtagatt attagaaaga gcagcaaaat
     7381 taaacgatga cttaggtggc ggttcaatta ctgcattacc aattatcgaa acacaagctg
     7441 gtgatatttc agcttatgta ccaacaaacg ttatttcaat tacagatgga caaatcttct
     7501 tacaatctga tttattcttc tcaggtgtaa gaccagcgat taatgctgga caatctgtat
     7561 ctcgtgttgg tggatctgca caaattaaag caatgaagaa agttgctggt acgttacgtc
     7621 ttgacttagc gtcatacaga gaacttgaat catttgcaca attcggttca gaccttgatg
     7681 aatttactgc aagtaaatta gaacgtggta aacgtactgt tgaagtctta aaacaagatc
     7741 aaaacaaacc attaccagtc gaacaccaag tgttgattat ttatgcatta acaaaaggat
     7801 atttagatga tattccagtt gtagatatca cacgttttga agatgagtta aaccactggg
     7861 cagaatcaaa tgctactgaa ctgttaaatg aaatcagaga aactggtggc ttaccagatg
     7921 ctgagaagtt tgacacagca attaacgaat tcaaaaaaag ctttagcaaa tctgaataat
     7981 aaacaagttt agtataaggt ggtgagatag tggcttctct taaagaaata gatactcgaa
     8041 taaaatcaac caaaaaaatg aagcagatta cgaaagcgat gaacatggta tcaagttcaa
     8101 aacttcgtag agctgaaaaa aatacaaaac aattcacacc atatatggat aaaatgcaag
     8161 atgcaattac tgcagttgca ggggcaagta gcaatacaaa ccatccaatg ctaagacctc
     8221 gtaaaattac tagaagtgga tatttagtta tcacgagtga taaaggttta gcaggtgcat
     8281 atagtgcaaa cgtgcttaaa aaattgatta ctgatattga agcgaaacat caagatagta
     8341 gcgaatacag tattgtagtt ttagggcaac aaggtgttga tttcctaaaa aatagaggtt
     8401 atgacattga gtattctcaa gtagacgtac ctgatcaacc ttctttcaaa tctgttcaag
     8461 cactagctaa ccatgctata gacttataca gtgaagaaga aattgatgaa ttaaatatat
     8521 actatagtca ttatgtcagc gttcttgaaa acaagcctac atctagacaa gtattaccat
     8581 tatctcaaga ggattctagt aaggggcatg gtcatttgtc ttcttatgaa tttgagccag
     8641 ataaagaatc tatcttaagt gtaatcttgc ctcaatatgt tgagagtttg atttacggaa
     8701 caatattaga cgcaaaagca agtgagcatg caacacgtat gactgcgatg aaaaatgcca
     8761 ctgataatgc aactgaactt attgatgact tatcattaga atataacaga gcgagacaag
     8821 cagaaattac gcaacaaatt actgaaattg ttggtggttc cgcagcgctt gaataatatt
     8881 taaaggagga aaatagcatg ggaattggcc gtgtaactca agttatgggt cctgtaattg
     8941 atgttcgatt taaacataac gaagttccta aaattaataa cgccttggtt attgatgtgc
     9001 ctaaagaaga aggtacaata caactaacat tagaagttgc gctgcaatta ggtgacgacg
     9061 ttgttcgtac aattgcgatg gattcaactg atggtgtcca aagaggcatg gatgtaaaag
     9121 atacaggcaa agaaattagt gtacctgttg gtgacgaaac attaggtcgt gtatttaatg
     9181 tactaggtga aacaattgac cttaaagaag aaattagtga ttctgttcgc cgcgatccta
     9241 tccatcgtca agcaccagca ttcgatgaac tttcaacaga agttcaaatt ttagaaacag
     9301 gtattaaagt agtagattta ctagcacctt atattaaagg tggtaaaatc ggattgttcg
     9361 gtggtgccgg tgtaggtaaa acagtattaa tccaagaatt aattaacaac atcgctcaag
     9421 agcacggtgg tatttctgta ttcgccggtg taggtgaacg tactcgtgaa ggtaacgatt
     9481 tatacttcga aatgagtgac agtggtgtaa ttaagaaaac agccatggta ttcgggcaaa
     9541 tgaatgagcc acctggtgca cgtatgcgtg ttgcattatc tggtttaaca atggctgaat
     9601 atttccgtga cgaacaaggt caagacgtat tattattcat cgataacatt ttcagattta
     9661 cacaagctgg ttctgaggta tctgcattat taggtcgtat gccttctgca gtaggttacc
     9721 aaccaacact tgctactgaa atgggacaat tacaagaacg tattacgtct acaacaaaag
     9781 gatcagttac ttctattcaa gcggtattcg tacctgccga tgactatact gacccagcgc
     9841 ctgcgacagc gtttgcccat ttagatgcaa ctacaaactt agaacgtaaa ttaactgaaa
     9901 tgggtattta tccagccgtg gatccattag cgtctacatc aagagcattg gaaccatcaa
     9961 ttgtaggtca agaacattat gaagtagctc gtgatgtaca atcaacactt caaaaatacc
    10021 gtgaattaca agatatcatt gctatcttag gtatggacga attatctgat gaagataaac
    10081 aaacagttga acgcgcacgt agaattcaat tcttcttatc tcaaaacttc cacgtagcgg
    10141 aacaatttac tggtcaaaaa ggttcttatg tacctgttaa gacaacagtt gcaaacttta
    10201 aagatatctt agatggtaaa tatgaccata ttccagaaga tgctttccgt ttagttggta
    10261 gcatggatga tgttattgca aaagctaaag atatgggtgt tgaagtataa caattaggag
    10321 gaatggataa tgaatacatt aaacctagat attgtcactc ctaatggttc tgtttacaat
    10381 cgtgataatg ttgaactcgt tgttatgcaa acaacagctg gtgagatagg tgtcatgagt
    10441 ggacatattc caactgtagc tgctttaaaa acaggctttg taaaagtgaa atttcacgat
    10501 ggaactgaat atattgctgt aagcgatggc tttgttgaag ttagaaaaga taaagtttca
    10561 atcattgttc agactgcaga aactgcaaga gaaattgatg ttgaaagagc taaattagcc
    10621 aaagcaagag cagagtctca cttggaaaat gatgacgaca atactgatat tcatagagcc
    10681 gaaagagctt tagagagagc aaataaccgt ttgcgtgtgg ctgaattaaa atagtaaata
    10741 aagggtcgaa gatgtgattt catatcttcg accctttttt gaattatatt gatttaaaga
    10801 tacaaaacat gagagggggg aaggaattga taaagaacta ttaaagattt atgatgtagt
    10861 ggttctttat cattaaacac agctaatgtg tatttaaaaa taggaataca tgagtaaaac
    10921 tcatgtataa gaaatactaa tttctaaaga aaaagtattt ctttatgttg gggccccgtc
    10981 aactactgcc aaatacaaca ctatagagtc tagacattga tttatgtccg actcccaaga
    11041 atagttttac ttttttacaa tcactaatag attgctaaaa tcaaaatttc attcaccact
    11101 atctacagtc gacatttcat tttttgaaag tagctacatt ttttcatacc aagatacttt
    11161 atagttatga tatttatgta aaaagaatta tatagtaagt tagcttaaac tttactaaaa
    11221 acgggtatta aactttgtat cattatttaa atttttcatg tacaatgtaa tacagtaatc
    11281 ttatgaggtg ataaaatgga ttatatcgga caatatgcag ttatccattt agtgttacat
    11341 gttgtatgta tttgtattgc ctattgggct ttacaatcaa ttagattaga tcaatttttt
    11401 aaaaaaggat acgccactca attacaagtg tgtatgatat ttgttgctat tttattaggc
    11461 actgcagtaa gcaattttat tgtagatttg ttacaatact cgacgcaggt aaaatattta
    11521 ataaaataag tctaactcta tgatttgtaa tcaaaactag atataattaa ataatgactt
    11581 aaaataattt taaaataggg aaatgtaaag taataggagt tctaagtgga ggatttacga
    11641 tggataaaat agtaatcaaa ggtggaaata aattaacggg tgaagttaaa gtagaaggtg
    11701 ctaaaaatgc agtattacca atattgacag catctttatt agcttctgat aaaccgagca
    11761 aattagttaa tgttccagct ttaagtgatg tagaaacaat aaataatgta ttaacaactt
    11821 taaatgctga cgttacatac aaaaaggacg aaaatgctgt tgtcgttgat gcaacaaaga
    11881 ctctaaatga agaggcacca tatgaatatg ttagtaaaat gcgtgcaagt attttagtta
    11941 tgggacctct tttagcaaga ctaggacatg ctattgttgc attgcctggt ggttgtgcaa
    12001 ttggaagtag accgattgag caacacatta aaggttttga agctttaggc gcagaaattc
    12061 atcttgaaaa tggtaatatt tatgctaatg ctaaagatgg attaaaaggt acatcaattc
    12121 atttagattt tccaagtgta ggagcaacac aaaatattat tatggcagca tcattagcta
    12181 agggtaagac tttaattgaa aatgcagcta aagaacctga aattgtcgat ttagcaaact
    12241 acattaatga aatgggtggt agaattactg gtgctggtac agacacaatt acaatcaatg
    12301 gtgtagaatc attacatggt gtagaacatg ctatcattcc agatagaatt gaagcaggca
    12361 cattactaat cgctggtgct ataacgcgtg gtgatatttt tgtacgtggt gcaatcaaag
    12421 aacatatggc gagtttagtc tataaactag aagaaatggg cgttgaattg gactatcaag
    12481 aagatggtat tcgtgtacgt gctgaagggg aattacaacc tgtagacatc aaaactctac
    12541 cacatcctgg attcccgact gatatgcaat cacaaatgat ggcattgtta ttaacggcaa
    12601 atggtcataa agtcgtaacc gaaacagttt ttgaaaaccg ttttatgcat gttgcagagt
    12661 tcaaacgtat gaatgctaat atcaatgtag aaggtcgtag tgctaaactt gaaggtaaaa
    12721 gtcaattgca aggtgcacaa gttaaagcga ctgatttaag agcagcagcc gccttaattt
    12781 tagctggatt agttgctgat ggtaaaacaa gcgttactga attaacgcac ctagatagag
    12841 gctatgttga cttacacggt aaattgaagc aattaggtgc agacattgaa cgtattaacg
    12901 attaattcag taaattaata taatggagga tttcaaccat ggaaacaatt tttgattata
    12961 accaaattaa acaaattata cctcacagac agccattttt attaattgat aaagtagttg
    13021 aatatgaaga aggtcaacgt tgtgtggcta ttaaacaagt atcaggaaac gaaccattct
    13081 ttcaagggca ttttcctgag tatgcggtaa tgccaggcgt attaattact gaagcgttag
    13141 ctcaaacagg tgcggtagct attttaaata gtgaagaaaa taaaggtaaa atcgctttat
    13201 ttgctggtat tgataaatgt cgttttaaac gtcaagtagt acctggtgat actttaacgt
    13261 tggaagtaga aatcactaaa attaaaggac caatcggtaa aggtaatgct aaagctactg
    13321 tcgatggtca acttgcttgt agttgtgaac ttacatttgc aattcaagat gtaaaataaa
    13381 acaaaaaaac attcaaagat ttaatgtgtt ggcaaaatct ttgaatgttt tttattttac
    13441 tcttctaatt tttcatcctt taactttggt ttagactgca tcattcgatt aaatgatttt
    13501 tttaattctt caccagataa tccatcatca ataagttgtt ctaataaact ttcagcatac
    13561 tgttgacgta aagtgtaaat atgacattca aatacgatag acattgttgt tttaaaattt
    13621 ttaatacttt tcacagtaga ttcaaataaa gctggatcat tggctggacg cgtgatgacg
    13681 acacgaatat ccaataactg ttcattgtct tgatattcct taaatgtttc ataatgctca
    13741 ttagaaatga caatttcaag caaccaaccc gtgccacttt cctctttatt aattattacg
    13801 ccatcttcta attcatattc tataattctg ccgtgttcat tcacaatttg aaaccttact
    13861 gctttaaaag ttttcatatt cccacatccc attagtttaa tatttatgat ttttgaatta
    13921 tttagaagat ttaaggtcat ttcatgacta atttctttaa atataacaaa taacgagatg
    13981 ctatagctat taattatata aaatttgcca ttttgaccat ttttaagcta gtaattaaga
    14041 tataggcata ggaggtgaat cacacactac atgctaaata aaatcgtaat tgtcgagaga
    14101 ctgacgaaag acgcacaaat atttgaaaag gaggatagaa aaattgcaac gttttgtgtt
    14161 gcaacgcacc gaaattataa agatgaaaat ggagaaatcg tctgtgatta cttattctgt
    14221 aaagcatttg gcaagttagc ttctaatata gaaaaatata ctaatcaagg tacattggtt
    14281 ggtataactg gtcaaatgag atcaagaaag tatgataaag acggacaaac acactttgtc
    14341 actgaattat atgttgaaac aataaaattt atgtccccta aatcccaaaa taatgaaatt
    14401 ctctcagata gtattttaga tattgactct caaaatatag ataatcatga cttattagaa
    14461 atttaaatcc aattatccta aacatcctta atatacattt aatccatcct aaatttttta
    14521 tagatctaat taacttgaaa tatactcact taaaaacgac ttacatgact agtactgttg
    14581 tgtaggtcgt ctttttgtgt agtttttcta atagaaaata gacatagatt gcaaaacgat
    14641 tacataaaaa attgcaaata aaaaacattt ttactgtcat gacaattctc gatagaatca
    14701 tatctgaaat tatgggggat ttcgttgtaa tcactgtgta aaaaacaagg ctattttttt
    14761 attttgttgt aacctgcacg gattttatag atgttacagt agtctctgta aaacttttag
    14821 agaaacaaat tacttgtagg aggattttaa gattatgaag aaaacattac tcgcatcatc
    14881 attagcagta ggtttaggaa tcgtagcagg aaatgcaggt cacgaagccc atgcaagtga
    14941 agcggactta aataaagcat ctttagcgca aatggcgcaa tcaaatgatc aaacattaaa
    15001 tcaaaaacca attgaagctg gggcttataa ttatacattt gactatgaag ggtttactta
    15061 tcactttgaa tcagatggta cacactttgc ttggaattac catgcaacag gtactaatgg
    15121 agcagacatg agtgcacaag cacctgcaac taataatgtt gcaccatcag ctgttcaagc
    15181 taatcaagta caatcacaag aagttgaagc accacaaaat gctcaaactc aacaaccaca
    15241 agcatcaaca tcaaacaatt cacaagttac tgcaacacca actgaatcaa aatcatcaga
    15301 aggttcatca gtaaatgtga atgctcatct aaaacaaatt gctcaacgtg aatcaggtgg
    15361 caatattcat gctgtaaatc caacatcagg tgcagctggt aagtatcaat tcttacaatc
    15421 aacttgggat tcagtagcac ctgctaaata taaaggtgta tcaccagcaa atgctcctga
    15481 aagtgttcaa gatgccgcag cagtaaaatt atataacact ggtggcgctg gacattgggt
    15541 tactgcataa gccatttatg catagctaat taatagttat ataagtaact tttagattgg
    15601 aatatatcgg ggaattctcg atatgttccg gtttttttat ttcgcaaata tatgtcatta
    15661 tacaggctgg gacataaatc aatgttctat gctctacgaa gttatattgg cagtagttga
    15721 ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct agtcaacctt gccggggtgg
    15781 gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccaact gagcaacaat
    15841 aattgaaaat gttgaaaccg tttgctaaga ttaatttgca attgaatact aaggaactac
    15901 taggggagcc taatgatatg gctgagatga attgttcaga cccttatgac ctgatttggt
    15961 tagtaccaac gtaggaaagt agttattgaa gtgatggggc tagtatagca acattgccat
    16021 caaactactt tcatgttgga agtagttttt ttatttagga aggatttttt acaatggaat
    16081 tttcacaaaa attgtaccaa gctgcaaagc caatcattaa tgatatttat gaggatgact
    16141 ttattcagaa gatgttatcc ggagatatag gagctgacgc attacgccat tacttaaaag
    16201 ctgatgctgc gtatttaaaa gaatttacaa acttatatgc tttgttaatt ccaaaaatga
    16261 atagcatgaa tgacgtaaaa tttttagtag agcaaataga atttatggtt gaaggtgaag
    16321 tacttgcgca tgatatttta gcgcaaattg ttggtgaatc atacgaagaa atcattaaaa
    16381 caaaagtttg gcctccaagt ggcgaccatt acataaaaca tatgtatttc caagcgcata
    16441 gtcgtgaaaa tgctatttat acaattgctg caatggcacc ttgtccatat atttatgcag
    16501 aattagctaa gcgctctcaa agtgatcata agcttaatag agaaaaagat acggcaaaat
    16561 ggtttgattt ttatagtact gaaatggatg acataattaa cgtatttgag gcattaatga
    16621 ataaattagc cgaatcaatg tctgacaagg aattggaaca agtaaagcaa gtatttttag
    16681 aaagttgcat acatgagcgt cgatttttca atatggctat gacactagaa caatgggaat
    16741 ttggaggaaa agtaaatgat taaacctaaa atagcattaa ccattgcagg tactgatcca
    16801 acaggtggtg ccggcgtaat ggctgattta aaatcatttc attcatgtgg tgtatatggt
    16861 atgggcgtcg ttacaagtat tgttgctcaa aatacattgg gcgtacaaca tattcataat
    16921 ttaaatcatc aatgggtaga tgaacaactt gatagtgtct tcaatgatac cttacctcat
    16981 gctattaaaa cggggatgat tgctacagca gatactatgg aaacgattcg tcattattta
    17041 atgcaacatg aatctattcc atatgtaatt gatcctgtta tgttggcgaa aagcggtgat
    17101 tcattaatgg ataatgacac aaagcaaaac ttgcagcata cgttattgcc attagctgac
    17161 gtagtaacac cgaatttacc agaagctgaa gaaataacgg gactaaccat tgatagtgaa
    17221 gaaaaaatta tgcaggctgg ccgcatcttt attaatgaga ttggtagtaa aggtgtcatc
    17281 attaaaggcg gtcattcaaa tgatactgat atagcaaaag attatttatt tactaacgaa
    17341 ggtgttcaaa catttgaaaa tgaacgattt aaaacaaaac atacgcatgg aacagggtgt
    17401 acattttcag cagttataac ggcagaactt gcaaaaggta gacgattatt tgaggctgta
    17461 cacaaggcta aaaagtttat ttcaatgagt atacaatata cgcctgaaat cggccgtggt
    17521 agaggtccag tgaatcattt tgcatattta aagaaagagg gattagacga tgaattatct
    17581 aaataaaata cgtattgaaa acccattaac aatttgttat acaaacgatg tagttaaaaa
    17641 ttttacagcg aatggtttat taagtattgg tgctagccct gcaatgagtg aagctcccga
    17701 agaagctgaa gaattttaca aagttgcaca agggctatta atcaatatcg gtactttaac
    17761 agcagaaaat gaacaagata ttattgcgat tgctcaaacg gcaaatgagg caggcttacc
    17821 tattgtattt gaccctgtag ctgttggtgc ttctacatat cgaaagcaat tttgtaaatt
    17881 attattgaaa tcagcgaaag tatcagtaat taaaggcaat gcatctgaaa tattagcgtt
    17941 gattgatgat acagcaacta tgaaaggtac agatagtgat gctaatcttg atgcggttac
    18001 aatagcgaaa aaggcttacg caacatataa aactgcaata gtaatcacag gtaaagagga
    18061 cgttattgtt caagataata aagccatcgt attagctaat ggatctccat tattagcacg
    18121 agtaactgga gctggttgtt tattaggagg cgttattgct ggatttttat ttagagaaac
    18181 agaaccagac atagaagcgt taattgaagc ggtaagcgta tttaatattg ctgctgaggt
    18241 agctgctgaa aatgaaaatt gtggtggtcc tggtacgttt tcaccattgt tgcttgatac
    18301 gttatatcat ttaaatgaaa caacctatca acaacggatt cgtattcaag aggtggaaaa
    18361 atatgtttaa ccaatcgtat ctaaatgtgt attttatttg tgggacctcc gatgttccga
    18421 gtcatcgaac tattcatgaa gtattagaag cagcattaaa ggcagggatt acacttttcc
    18481 aatttcgaga aaagggtgaa tcagctttaa aaggaaacga taaacttgta ttagctaaag
    18541 aattacagca tctctgtcat caatatgatg tgccttttat cgttaatgat gatgtatcct
    18601 tggcaaaaga aattaacgcg gatggtatac atgtcggtca agatgatgcg aaagtcaaag
    18661 agatagcaca atatttcact gataaaatta ttggacttag tattagtgat ttagatgagt
    18721 atgcaaaatc tgatttaact catgttgatt atataggtgt tggaccaatc tatcctacgc
    18781 cgtcgaagca tgacgcgcat ataccagtag gtccagaaat gattgcaacg tttaaagaaa
    18841 tgaatccgca actaccgatt gtagctattg gcggtatcaa tactaataat gtggcgccga
    18901 ttgtagaagc aggtgccaat ggaatttcag tcatttcagc catttctaaa agtgaaaata
    18961 ttgaaaatac tgttaatcga ttcaaagatt tttttaataa ttagttaaat ttttccactt
    19021 attgtttcat acgtactcat taatgtgata gaataaatcc tatgtgaata taatgataga
    19081 ggtggaaaaa tgaagaaaaa agcgttacta ccattatttt taggtattat ggtctttttg
    19141 gctggttgtg actattctaa acctgaaaaa cgtagtgggt ttttctacaa tacattcgta
    19201 gatccaatga aaaatgtatt ggattggttg ggaaataact tattaaacga caattatggt
    19261 ttagctatta ttatccttgt attggtaatt cgtattattt tattaccatt catgttgtca
    19321 aactataaaa atagtcatat gatgcgtcaa aaaatgaaag ttgcaaagcc agaagttgaa
    19381 aaaattcaag aaaaagtgaa acgtgcgcgt acacaagaag aaaaaatggc tgcaaaccaa
    19441 gaattaatgc aagtatataa aaagtatgac atgaacccga ttaagagtat gttgggttgt
    19501 ttaccaatgc taatccaatt gccaatcatc atgggattat actttgtact taaagaccaa
    19561 cttgtagatg gtttgtttaa atatccacac ttcttatggt tcgatttagg acgtcctgat
    19621 atttggatta caattattgc cggtgtttta tactttatcc aagcatatgt atcaagtaaa
    19681 acgatgccag acgaacaacg tcaaatgggt tacatgatga tggtcatttc accaattatg
    19741 attatctgga tttcattaag ctcagcatca gcacttggtt tgtactggtc agtcagtgcg
    19801 gcgttccttg tagttcaaac acactttgcg aacatttatt atgaaaaagt cgctaaaaaa
    19861 gaagtacaac ctttcattga agcgtatgaa agagagcaca acggcggcag caataaaaaa
    19921 ggcaaaaaca cacaagtagt gtctaaaaag aaaaagaaat aaccttagca agtccaaata
    19981 atgtgcaccc taaaagttga atataatttt aactttatag ggcgcacatt atttttcgct
    20041 tcaaaagcga aacttagtgt cacattatac aatatgtgat aaatagaaac agcagtaaga
    20101 tgatttcaaa tgtgaaagtc atcctactgc tgtttttatt tgaaataata ttaacgagtt
    20161 ttcagtatgt aaaaagtggt taataacaga gttgtcattc ttcatcgcaa tgtcatcttg
    20221 acacaatgat tcatcagtgt agcagtatat gtcgcaaatt tccacagcaa ttcacttata
    20281 catgtaacag tgtgaaataa taacgtcaca acggcataat acacattgca acgtcccaac
    20341 aacatatcat agaatcgtaa cggcatggca tcgacgccaa accggtctat aacaccataa
    20401 caccgcaatg ccacatcata gcactacatc aagtcgcatc gtagcaccag cacatcaaag
    20461 taacatggca tcaacaccaa gcttccacaa caacacatca aagtaacatg gcatgacacc
    20521 aagcttccac aacaacacgc cattacatcg catcatcata ctacaacttc aatgtctagt
    20581 cgtgataatt ccattccgta aaaaactgtt tcaagtacat catcataaag tcgtgacgtt
    20641 ctttagcaat catcttcgcc gtatctgtat gcattaaaga ttctaactta agtaattttt
    20701 caaagaaatg tttaattgca gatggtggca actgttcaac taaatcatca ttaatcttat
    20761 ctagtgacat atgttctgtc cacattggtt caccaaagtg tcctgcaaat tgaaatgttc
    20821 gtgcaacacc tatagcgcct atagcatcaa gacgatctgc atccctgaca atttgacctt
    20881 ctaaagataa agtgacatga tcatttttgc cattgcgata gctcatatta ttaataataa
    20941 ataaaatgtg ctcttgatct tcggttgata gtgataaaga agataaaaat gacttcaatt
    21001 caacatatag tttgttagca tctacaactt tttcgtcaac ggtatcatga agcaaacatg
    21061 cgagttcaat gactaaagta tcattaacac cttcattttc agcgattgat ttagctaaag
    21121 cagtgacacg atatacatgc gcaatatcat ggcctgtata atcattttga tgaatagatt
    21181 ccatatattt tttggcagca ctaatgacgt ctgattgttg cattaaagtt acactcctca
    21241 tatttctatt ttataagata ggtgacaata attgtgaaat accttcttta aatttaatcc
    21301 aaagacttcg cttagcataa cgtgcttttg ttaattcaga agatactgct aaatcatcta
    21361 taaaagcttg ttttaatttt ttggcaattt gttggtcata aataaaggcg ttgacttcga
    21421 aattcaatgt gaaactgcga tggtccatat tagctgttcc aacacttgca atttcatcat
    21481 ctataacaag tgtttttgag tgtaaaaagc cattgtcgta gtgaaatact ttaacaccgg
    21541 catctaataa ggatgctgca ttttttaaag tagcccaaaa aacaaacgga tggtcaggtt
    21601 tattaggaat catgatattg acatcaacac cacctaatgc cgcaatttta atagaatcta
    21661 aaaaggcttg atcaggtata aaatagggag attgaatata aatcgatttt ttagcagatg
    21721 aaatcatttt caaataaccg tatttaatct gttcccattc ttcgtcagga ccactagaag
    21781 ctatttgaac gccaattgtt ccaccagaat ttacatctgg gaaataacga tcatcatagg
    21841 agatgtggtc acgtgtggct tgtgaattcc aatctagaat aaatcgtaat tgcaatgcat
    21901 tcactgcgtc cccgacaatt cgtaaatgcg tatctcgcca atagccgaat ttttttgatt
    21961 tacctaagta ctcatcacca acattaaaac caccaacata tccaatttgc ccatctatta
    22021 caacaatttt tcgatggttt cgattgttca tacgcaagtt aattaaaggt aattttgatg
    22081 ggaaaaatgc ttcagcatgt ccacctttat tgcgaaacgg gcgtaagcct tttttacgca
    22141 gtccacgaga acccatgtca tcataaagaa ttttaacttc tacaccttgt tccgcttttt
    22201 taccaagttc atttaaaatg gtacgaccta attcatcatt ttgaataata tagtactgaa
    22261 aatgaatata atcagtagca ttacggatgt cttgtattag gtcatcaaat ttttcttggc
    22321 cgtctgtgta tatttttaaa tcattgtctg ttgttaaaaa tgctgcgtta ttatataaca
    22381 acatttgaat catttcttta aattttacaa tttgataatt ggaatttgaa aagttttcat
    22441 tttttaaagc agctaattgc tcatcaacga ttaactctaa tcctttttta tcttccttat
    22501 caattttgaa aatttggtca cgttgaattt gtcgtcctaa tagtaagtat aaaatgaagc
    22561 cgaataaagg caagaaaact aagactagta accaagccca gatagaattg gcagaacgtc
    22621 tttccatgaa aataatggta aaggcgaata ataagtttaa aataaatgca ccaataaata
    22681 ttgaatttaa aataatatta gaatgtttga gtgcaatgga taataactct atcatttgaa
    22741 acctcccatc gaaaatctta aacgagtagt atatcttatt aattgtatca tgaagcaatt
    22801 taaatatcat gacaatttag tttgaaaaac acactttgtg ttgacgttgt gttttatggg
    22861 atatagtata gtaaagaatg taaatgaagg agtgaatgct atgactgaac aagataatgc
    22921 acatcattct gaacaaataa aaacgaatct taaatcacgt ttaaatcgaa ttgaaggaca
    22981 agtgagagcg attaatcgca tgattgaaga agatgtctat tgtgatgatg tccttacgca
    23041 aataagagcg acacgttcgg cgttaaacag tgttgcgata aagttattag aacaacatat
    23101 gaaaagttgt attatgaata aagttaatca aggtgctcag gaagaggcga tggaagagtt
    23161 attagtgact tttcaaaaat tgattaaaga ctaaaggagt ttaagatgat acatcaaaat
    23221 acgatttaca cagcgggaat tgaaacagaa gaacaagtaa gtcaattgac agaacgcatt
    23281 tcaaatatga taggtgttca tcaagtgaat attaatataa tagatggtca agtaactgta
    23341 tcgtatgaga caccagcaaa tttgaatagt attgaaaaag aaatctatga tgaaggatac
    23401 aaaattgtaa tttagggtat aatgtagagt gcgctataga ttttaatttt gaaaataaat
    23461 taaaaatttt gtaaatgatg tagtaaaggt atgtcgaata gggtaagata atattgaaag
    23521 cgatccaaac gaggtgataa taatgaaacg tcctgaaaag attcaaaatg tagtcaaact
    23581 attgtcatca ttaggtgtga atattaaaaa aactaaatct cgtttagaca ttattaatac
    23641 tttgccagca tctaataaag taagtcacga attaaaataa ttgactgtga ttgtgaatca
    23701 cacaccgtta aagctatcgt tggactattc gatgatagct tttttgactg gatttactaa
    23761 aatgaaagca agtagtatat aactaaatcc ctctttagag tatacatttt acatgtgtac
    23821 ctcaaagagg gattgttatt atcgagtaat atctatatat gacattttaa atcgacgata
    23881 taaatagact atataatgtc atcttgaaaa gttatgatat ttgaaactca aaatagttta
    23941 aattaattac tttttggatg gtataaatcg acatatcgtt ttggttcatg ataataaatt
    24001 gataagacta tacctattcc agtcatcata ctccatagcg cactaccacc ataactaata
    24061 aatggtaatg gaataccagt gattggtaac aactgaattg tcataccaat attttgtaaa
    24121 atatggaaca caagtaaagt aacgaaacca acgataaaga ttttgttaaa ttgatcttca
    24181 attttcgcag ctaatcttat tagatggaag attaaaaata aaaatattaa gatcaatatg
    24241 acagaaccga taaaacctag ttcctctcca atcactgaaa agataaagtc agtatgattt
    24301 tcaggtatat aaacttcacc gtgattgtat cctttaccta gtaactgtcc agaaccgata
    24361 gctttaagtg attcagttaa atgatagcca tcgccactac tatatgtata ggggtcaagc
    24421 catgaattga ttcgtcccat ttgatacagt tggacaccta ataaattttc aattaatgcg
    24481 ggtgcatata gaatacctaa aatgactgtc attgcaccaa caatacctgt aataaagata
    24541 ggtgctaaga tacgccatgt tataccactt actaacatca cacctgcaat aattgcagct
    24601 aatactaatg tagttcctag gtcattttgc agtaatatta aaatacttgg tactaacgag
    24661 acaccaataa ttttgaaaaa taataacaaa tcactttgga atgatttatt gaatgtgaat
    24721 tgattatgtc tagaaacgac acgcgctaat gctaaaatta aaataatttt catgaattca
    24781 gatggctgaa tactgatagg gccaaacgtg taccaacttt tggcaccatt gataataggt
    24841 gtaataggtg actcaggaat aacgagcaag cctattaata atagacagat taagaaatac
    24901 aataaatatg tataatgttt aatcttttta ggtgaaataa acatgatgat acctgcaaaa
    24961 attgcaccta aaatgtaata aaaaatttgt ctgataccga aattagcact gtattgacca
    25021 ccgcccattg ccgagttaat aagcagaaca ctgaaaattg ctaaaacagc tatagtggct
    25081 actaataccc agtctacttt gcgaagccaa tgcttatccg gctgttgacg agatgaataa
    25141 ttcattgcaa actcctttta tactcactaa tgtttatatc aattttacat gactttttaa
    25201 aaattagcta gaatatcaca gtgatatcag ctatagattt caatttgaat taggaataaa
    25261 atagagggga atattgttct gattataaat gaatcaacat agatacagac acataacgcc
    25321 tcgtttttaa aatgcaaaat agcattaaaa tgtgatacta ttaagattca aagatgcgaa
    25381 taaatcaata ttaatttata ttaaggtagc aaaccctgat atatcattgg aggaaaacga
    25441 aatgacaaaa gaaaatattt gtatcgtttt tggagggaaa agtgcagaac acgaagtatc
    25501 gattctgaca gcacaaaatg tattaaatgc aatagataaa gacaaatatc atgttgatat
    25561 catttatatt accaatgatg gtgattggag aaagcaaaat aatattacag ctgaaattat
    25621 atctactgat gagcttcatt tagaaaatgg agaggcgctt gagatttcac agctattgaa
    25681 agaaagtagt tcaggacaac catacgatgc agtattccca ttattacatg gtcctaatgg
    25741 tgaagatgga acgattcaag gactttttga agttttggat gtaccatatg taggaaatgg
    25801 tgtattgtca gctgcaagtt ctatggacaa acttgtaatg aaacaattat ttgaacatcg
    25861 agggttacca cagttacctt atattagttt cttacgttct gaatatgaaa aatatgaaca
    25921 taacatttta aaattagtaa atgataaatt aaattaccca gtctttgtta aacctgctaa
    25981 cttagggtca agtgtaggta tcagtaaatg taataatgaa gcggaactta aagaaggtat
    26041 taaagaagca ttccaatttg accgtaagct tgttatagaa caaggcgtta acgcacgtga
    26101 aattgaagta gcagttttag gaaatgacta tcctgaagcg acatggccag gtgaagtcgt
    26161 aaaagatgtc gcgttttacg attacaaatc aaaatataaa gatggtaagg ttcaattaca
    26221 aattccagct gacttagacg aagatgttca attaacgctt agaaatatgg cattagaggc
    26281 attcaaagcg acagattgtt ctggtttagt ccgtgcagat ttctttgtaa cagaagacaa
    26341 ccaaatatat attaatgaaa caaatgcaat gcctggattt acggctttca gtatgtatcc
    26401 aaagttatgg gaaaatatgg gcttatctta tccagaactg attacaaaac ttatcgagct
    26461 tgctaaagaa cgtcaccagg ataaacagaa aaataaatac aaaattgact aactgaggtt
    26521 gttattatga ttaatgttac attaaagcaa attcaatcat ggattccttg tgaaattgaa
    26581 gatcaatttt taaatcaagt gataaatgga gtcacaattg attcacgagc aatttctaaa
    26641 aatatgttat ttataccatt taaaggtgaa aatgttgacg gtcatcgctt tgtctctaaa
    26701 gcattacaag atggtgctgg agctgctttt tatcaaagag ggacacctat agatgaaaat
    26761 gtaagcgggc ctattatatg ggttgaagac acattaacgg cattacaaca attggcacaa
    26821 gcttacttga gacatgtaaa ccctaaagta attgccgtca cagggtctaa tggtaaaaca
    26881 acgactaaag atatgattga aagtgtattg cataccgaat ttaaagttaa gaaaacgcaa
    26941 ggtaattaca ataatgaaat tggtttacct ttaactattt tggaattaga taatgatact
    27001 gaaatatcaa tattggagat ggggatgtca ggtttccatg aaattgaatt tctgtcaaac
    27061 ctcgctcaac cagatattgc agttataact aatattggtg agtcacatat gcaagattta
    27121 ggttcgcgcg aggggattgc taaagctaaa tctgaaatta caataggttt aaaagataat
    27181 ggtacgttta tatatgatgg cgatgagcca ttattgaaac cgcatgttaa agaagttgaa
    27241 aatgcaaaat gtattagtat cggtgttgct actgataatg cattagtttg ttctgttgat
    27301 gatagagata ctacaggtat ttcatttacg attaataata aagaacatta tgatctacca
    27361 atattaggaa agcataatat gaaaaatgcg acgattgcca ttgcggttgg tcatgaatta
    27421 ggtttgacat ataacacaat ctatcaaaat ttaaaaaatg tcagcttaac tggtatgcgt
    27481 atggagcaac atacattaga aaatgatatt actgtgataa atgatgccta taatgcaagt
    27541 cctacaagta tgagagcagc tattgataca ctgagtactt tgacagggcg tcgcattcta
    27601 attttaggag atgttttaga attaggtgaa aatagcaaag aaatgcatat cggtgtaggt
    27661 aattatttag aagaaaagca tatagatgtg ttgtatacgt ttggtaatga agcgaagtat
    27721 atttatgatt cgggccagca acatgtcgaa aaagcacaac acttcaattc taaagacgat
    27781 atgatagaag ttttaacaag cgatttaaaa gcgcatgacc gtgtattagt taaaggatca
    27841 cgtggtatga aattagaaga agtggtaaat gctttaattt catagagatt agtcgaggga
    27901 ccttttactt ataaaaatga tttgaattaa tactaaaaga ttacaaagaa gaggtggttt
    27961 tgtgtgtaaa tacaaaattg cctttttctt tttatgttaa atctataaat ttgaaactaa
    28021 atcaaggtta attctatgta cacactttat ataggaagta gtttgaatgt ttatataatg
    28081 ttttacaaaa agatgtagta ttataatgtc taatttcaca tgtgtttcag taaaatttgt
    28141 tgtggaatgt taacgatata cgtattttat aaaaaatttt ttataatgat tattcgaatg
    28201 atgcgtaacg cttacatctt atctaatgct agctttttga caaaaatatg acaatcaatt
    28261 aatgtgattc taataaatat tcgcaaattg ctttattgcg attaaatttt tttggtggta
    28321 ctatatagaa gttgatgaaa tattaatgaa cttatatgca aaagtatatt gagaaataaa
    28381 caggtaaaaa ggagaattat tttgcaaaat tttaaagaac tagggatttc ggataatacg
    28441 gttcagtcac ttgaatcaat gggatttaaa gagccgacac ctatccaaaa agacagtatc
    28501 ccttatgcgt tacaaggaat tgatatcctt gggcaagctc aaaccggtac aggtaaaaca
    28561 ggagcattcg gtattccttt aattgagaaa gtagtaggga aacaaggggt tcaatcgttg
    28621 attttagcac ctacaagaga attggcaatg caggtagctg aacaattaag agaatttagc
    28681 cgtggacaag gtgtccaagt tgttactgta ttcggtggta tgcctatcga acgccaaatt
    28741 aaagccttga aaaaaggccc acaaatcgta gtcggaacac ctgggcgtgt tatcgaccat
    28801 ttaaatcgtc gcacattaaa aacggacgga attcatactt tgattttaga tgaagctgat
    28861 gaaatgatga atatgggatt catcgatgat atgagattta ttatggataa aattccagca
    28921 gtacaacgtc aaacaatgtt gttctcagct acaatgccta aagcaatcca agctttagta
    28981 caacaattta tgaaatcacc aaaaatcatt aagacaatga ataatgaaat gtctgatcca
    29041 caaatcgaag aattctatac aattgttaaa gaattagaga aatttgatac atttacaaat
    29101 ttcctagatg ttcatcaacc tgaattagca atcgtattcg gacgtacaaa acgtcgtgtt
    29161 gatgaattaa caagtgcttt gatttctaaa ggatataaag ctgaaggctt acatggtgat
    29221 attacacaag cgaaacgttt agaagtatta aagaaattta aaaatgacca aattaatatt
    29281 ttagtcgcta ctgatgtagc agcaagagga ctagatattt ctggtgtgag tcatgtttat
    29341 aactttgata tacctcaaga tactgaaagc tatacacacc gtattggtcg tacgggtcgt
    29401 gctggtaaag aaggtatcgc ggtaacattt gtaaatccaa ttgaaatgga ttacattaga
    29461 caaattgaag atgctaatgg tagaaaaatg agcgcacttc gtccacctca tcgtaaagaa
    29521 gtacttcaag cacgtgaaga tgacatcaaa gaaaaagttg aaaactggat gtctaaagag
    29581 tcagaatcac gcttgaaacg catttctaca gagttgttaa atgaatataa cgatgttgat
    29641 ttagttgctg cacttttaca agagttagta gaagcgaacg atgaagttga agttcaatta
    29701 acttttgaaa aaccattatc tcgcaaaggc cgtaacggta aaccaagcgg ttctcgtaac
    29761 agaaatagta agcgtggtaa tcctaaattt gacagtaaga gtaaacgttc aaaaggatac
    29821 tcaagtaaaa agaaaagtac aaaaaaattc gaccgtaaag agaagagcag cggtggaagc
    29881 agacctatga aaggtcgcac atttgctgac catcaaaaat aatttataga ttaagagctt
    29941 aaagatgtaa tgtcttgagc tcttttttgt tttcaataat tgattctctg tagatatcaa
    30001 agtactaacg ttttaaaggt taaatattta attggattga gatctgtatg cggttatatc
    30061 attctgtgta aatatggttc tccaccaaat gtggtgagta tataatttaa agaactattt
    30121 ttaaattaag aataatcgaa cataaataaa ctttatgaaa tttcagtatc atgttcttgt
    30181 aaaaaaataa tagggctttt tgctgacgct agtgcgcgat aaataataag ttgaatataa
    30241 aaaagatcac tgccaatcat tcgtttaatg gcaggatctt ttttatttaa ttatttctct
    30301 ttccactgca acatttgata accaatgcgt ggatgtgttt taataatatc ttttgcgtcc
    30361 tcatgacatt gtgaaagttt ttgtcttaat gatgtcatat gcactcttaa actaggcatt
    30421 tcagtcttat taacatagcc atatattcgt tttaatatca tctcataagt gagtactttt
    30481 cctttatgat ttgacaatag ttctaacaag ctaaattcat ttggagtcaa atgtacctcc
    30541 tgattattaa taacaacaga tttggagcca aagtcgatgc ttagcaaacc gttagtaaat
    30601 acaatgttag tttcttgatg cgacttagcg attctctcga tgactcgtat tcgtgcccga
    30661 agctcatcaa cattaaaagg tttagtcata tagtcattcg caccgttatc taaagcttga
    30721 ataattgttt gttcttcttg tcttgcactt attacaatga taggaatgtc agtatgttgc
    30781 ctgatttctg aaatcaaaca taatccatct ttatctggta aacctaaatc taataaaatg
    30841 acatctggtt tatcaatttg aattttaaag tgtgcttgtg tggcattgtc ggctgtagtt
    30901 acattgtaat aatctaaagt taatgcaaca tcaagtaaat gtgtgattgc gtgatcatct
    30961 tcaattatca atattttaga ttgcattata cgtctccttc attaaagtct gtatctatat
    31021 tgaaatagaa tatactgccg tgtggttggt tcggtttata ttgtaagttt gattgatgtt
    31081 tgtgtaggat agtctgtact aaatataagc ctagtcccat gctttctttt tggttatctt
    31141 taaaatattt atttgatcct gtgtaaaaag gctcgaatat cttttgttgt tcttctaaac
    31201 taattccagg tccttcgtct ataacggcaa attcgatttg ttcatcgcta gcataacgaa
    31261 tagataaatt gattttggtg tcagtagaag tgtgtttaac tgcattttca atcaaattga
    31321 ataaagcttg taaaatcaac ttactgtcaa tgtgtataaa ctgtaaatct actgaggatg
    31381 atacagttat acgctttttt aaatggcgac gttctagaat catatcgatt tcttctacta
    31441 attcactaac gagataaggt tgcaatttta tctgaacatt tgatgactgt aattttgtta
    31501 atgataaaat atttgtcact aatagatata aatactgact ttcttgaaaa ctatgcacaa
    31561 gtaattgttc cttttctatg atagacatat ctttactatg tgatactaaa atatctaaat
    31621 ttcccataat tgttgttaac ggtgtacgta tgtcatgcga aattgatctt aaaaaatttg
    31681 aatgtgtcag ttgacgttca gcctgtaaca tggattctct cgtttgttta agtaacgtca
    31741 cattttcaac ggcgagagaa agttcattta acattgattc taatattgat gcatcatatg
    31801 gattaatcac ttgagaactt tggtaatcaa tggctagaat gcctttaatc ggagatgtgc
    31861 caattggtat caaccattta ttaatgcctg gaaatgtatc tgttgttgca ccagcttgtc
    31921 tttcattttt aattacccag cttaatgctt gttcatgctg ttgagtcgta ttatcgatat
    31981 ggttttgcaa tggtattgtt ttaattactt tcgattgatt gataacgtat atagtaattg
    32041 attgttgcaa taattgatta atttggtatc cagcatttat tagtaagttt tcaactgtat
    32101 aagtttgttt aatcgaatca ttaaattgaa ataataaatc tgtacgataa agttgctttt
    32161 tagtaatgga gtattggaat ttaatttgtt ttaataaagc actcgttaaa atacttgtta
    32221 aaatgctaac gataaatgta ataggatagt caaagcgata tacttcaaat gtatatctag
    32281 gttccgtaaa aaaataatta aaaacaaata cgttaataat tgctgctaaa aagccaatga
    32341 tgaaggatcg cgtccaaatg gataacaaaa tgatgccgat gagaaaaatc attaaaataa
    32401 tcgtactaga ctcatgctta tcaagttgat aaatccacag tcccatcatt acacaaatta
    32461 tttgaatcaa aatcattttt aacatatcta tggcgaaacg tttgccttta ggacgatagg
    32521 gtgatttgtt cattttcaat tcaacaggta tttgtttgat tggaacgatt tcgactttat
    32581 agctatgttc aaaggacatt aaatggtcaa ttaaaggtgt attgaaaaag tctcgccact
    32641 tatttctaat atgttgtccg ataattaatt tggttacatc ttgattttta caccattcgt
    32701 ctaatcctaa tgcaacggtt tggctataaa ctacttttac ttttgctcct aatgattttg
    32761 caagcatgag atgttgatgc acttgctttt gactatcttt atattgcctg tttttttcga
    32821 atacatctat ataaatagca gtgaatttcg catgttcttt ttgagcaata tggaatgcct
    32881 ctttaattac tgcttcatta taaatgctcc cactaattgc cacagcaata tgaggtttga
    32941 gtgacgtttt atgattgtgt cggacttttt ctttatcact catcaagtcg gcaactgttc
    33001 ttaacgtcaa tgtacgcagt tcgcttaggt gggcatacgt aaagaaatta ctaaatgcta
    33061 catctagcct atccttttta tataccttgc cagcttttaa gcgtttaatt aattgttcag
    33121 gtgagatatc tacgacttct aatacatcgg cgctcattat gaaatagtcg ggtacacgtt
    33181 ctttaacatg tataccggtc atcagttcaa tttgactact taaactttca atatgttgaa
    33241 tgttcaaagt ggtatgaaca tcgataccat gatttaaaat ttcttcaata tccatatatc
    33301 gtttctcatg acgatctcta gaaatattcg tatgtgctaa ctcatcgata agtactattg
    33361 tcggcgattc ttcgattatg cggtcgacat ctaaatattg aaaatgatga ctaccatgtt
    33421 tagtaaaatt ggttgtgatt ttaggtaatt gttcagctaa tgcatttgtt tcatcacgct
    33481 gatgcggttc aatatatcct attttaatat caacgttact ttgaaatagt tcaatggcat
    33541 ttgaaagcat ctcaaatgtt ttaccaacac ctggactgta accgatgtaa attgttaaag
    33601 atccacgctt ttttcctatg tttagcgatt cagtgtttga cataaccttc acctcgatag
    33661 caatatcata atattcttca caattaaatg ctataaaatt tatttctgca tacacatctt
    33721 aatgatttct taataactaa gtttgaaaat taatatttct ctcataaaca agttgttacg
    33781 atgttgttgt gttgtaagga ggttatgaca atgctcatcg ttttattact ggtagttatt
    33841 gcattagttt tgtatttatt ttatgcctta attcgtagtg aaaaatttta gaaatatatt
    33901 aagtgagggt tatcatggaa ataattttgt ttctaacaat gatggttatg attacgtatg
    33961 tttttagtgg atatctatat cgcgtagcat tggtgcaatc gtcaagagta gatttaatat
    34021 ttactagatt tgaaaatatg tgttttaaaa ttatcggcac agatttagaa cacatgtcag
    34081 ctaaaacata tgtcaaacat tttctggcat ttaatggatt tatggggttt ataacatttg
    34141 tattgctaat agttcagcaa tggctttttt taaatccaaa tcataattta aaccaatcga
    34201 tagatttagc gtttaataca gcaatatctt ttttaacaaa tagtaattta caacactata
    34261 acggtgaatc agatgtgaca tatttaacgc aaatgattgt aatgacatat ttaatgttta
    34321 catctagtgc atcaggttac gccgttttta tagcgatgtt gagacgttta actggattaa
    34381 ctaatatcat tggtaatttt tatcaagata ttgttcggtt tattgtccga gtacttttac
    34441 cattatcatg tttaattagt attttattga tgactcaagg tgtaccacaa acgttgcatg
    34501 ctaatttaat gattcggact ttaagcggac atattcaaca tattgcattt ggacctattg
    34561 catcacttga atcaataaaa catcttggta cgaatggtgg aggattttta gcaggaaatt
    34621 ctgcaacacc ttttgaaaat ccaaatattt ggagcaattt tatagaaatg ggcagtatga
    34681 tgttacttcc tatgtcaatg ttgttcttat ttggtcgcat gttaagtaga catggtaaac
    34741 gagtacatcg tcatgcgttg atattatttg tcgcaatgtt tttcattttt atagcaattc
    34801 ttacattaac tatgtggagt gagtatcgtg gtaatccaat actagcgaat ttaggcattt
    34861 atggaccgaa tatggaaggt aaagaggtac ggtttggagc aggcttgtca gcactattta
    34921 cagttattac gacggcattt acaacgggtt ctgttaataa catgcatgat agcttaacgc
    34981 ctttaggtgg attaggacca atggtattaa tgatgctaaa tgttgtattt ggtggcgaag
    35041 gcgtaggact catgaattta ttgatatatg tcttactgac ggtgtttata tgcagtttga
    35101 tggttggtaa aacaccagaa tatttaaata tgccaattgg cgcccgtgaa atgaaatgta
    35161 ttgtcttagt ctttctcata catccaattt taattttagt attttcagca cttgctttta
    35221 tgattcctgg agcaagtgaa agtataacga atccgtcttt tcatggtatt tcgcaagtta
    35281 tgtatgaaat gacatcagct gctgcgaaca atggatcagg gtttgaagga ctgaaagatg
    35341 atacaacatt ctggaatatc tctacaggaa tcattatgtt gctttctcgt tatataccaa
    35401 ttattttgca attaatgatt gcatcaagtt tagtgaataa aaaatcatac caccaagata
    35461 aatatacaat agcgattgat aaaccttatt ttggcgtatc actaatcgta tttatcgttt
    35521 tactaagtgg tttgacattt attccagtac tattacttgg tcctattggt gaatttttaa
    35581 ctttaaaata aagtaaaggg atggaaaaaa tgcatcatgt aaataaatat ttcaatcaaa
    35641 caatggtgat agaagctttg aaaatgagtt tttataagct gaatcctaaa caattaataa
    35701 aaaatccgat aatgtttgtc gttgaggttg gaatgatttt aacgttgata ttaatatgtt
    35761 ttcctgacat atttggaact agttatctat cgcgaggtta tttgattaca atttttatca
    35821 tattattaat aactatattg tttgctaatt tttcagaagc ctttgctgaa ggtcgtggaa
    35881 aagcgcaagc tgatagttta agacaagcac aatcaaattt aactgctcga ttgatagaag
    35941 aaaatggtgc gtatcggata gtgaacgcca cagagttaaa agccggacaa aatattcggg
    36001 tagaaaatgg agaaacaatc cctgctgatg gcgttgtaat aaatggatta gcaaccgttg
    36061 atgaatctgc aattactggt gagtctgcac ctgtgataaa agaatcaggt ggcgattttg
    36121 atggagtcat tggcggtaca cttgtaacat ctgattggtt agaaatacgt gttgagagtg
    36181 aagcgggtac atctttttta gataaaatga ttgccttagt tgaaggggcg gaaagaaata
    36241 aaacaccaaa tgaaattgca ctttttacat tattaacaac gttaacaatt atatttttag
    36301 tcgttattgt aacgttatat ccaatagcat cttacctgca tttaatttta cctattgcga
    36361 tgctcattgc gttaacagta tgtttaatac caacgacaat cggtggtttg ttatcggcga
    36421 ttggtattgc aggcatggat cgtgttactc aattcaatgt attagcgaaa agtgggcgtg
    36481 ctgtagaagt atgtggagat gtcgatgtaa tgattttaga caagacaggc acaatcactt
    36541 atggcaatcg aatcgctagt gaatttttac cagtgaatca acaaatgctg gaaaaattga
    36601 tagtcgctgc ttatatgtca tcaatctacg atgatacgcc agaaggaaaa agtattgtac
    36661 gattagcgaa gcagatgtat ataaatgagc tgcctaaaga cattgatggc acatataaga
    36721 cttttacagc tgaaactaga atgagcggaa tcattacaaa tgaaatatcg gtattcaaag
    36781 gtgcacctaa tagtatgata aatcttgtca aacaacaaca aggcaatatt cctcttaata
    36841 ttgagtctct ctgtatggat gtatcgagta aaggtggcac accattaata gtcattgaaa
    36901 acaatgtcat gcttggcgtg atttatttaa aagatgtgat taaagatggt cttgtagaac
    36961 gatttactga attgagaaaa atggggattg aaacagttat gtgtacaggt gataatgctt
    37021 taacagcagc tacaattgca aaagaagccg gggttgatag atttgttgca gagtgtaaac
    37081 ccgaagataa aattaaagtc atcaaagatg aacaagcgaa aggccatatt gttgcgatga
    37141 caggagatgg tacaaatgat gcacctgcgc tagcacaagc gaatatagga ttagctatga
    37201 actcagggac aattagtgct aaagaggcag ctaatctaat tgatttagat tcgaatccta
    37261 caaagttaat agaggttgta aaaataggca aacaattatt aatgacgaga ggtgcattaa
    37321 cgacatttag tttggctaat gacgtagcaa aatactttgc aattttacct gcactaatga
    37381 tgtcaacaat tccagaaatg acttcactaa atattatgca tttatcgtca cctaaatctg
    37441 caattatatc agctttgata ttcaatgcat taatcattgt tgccttaatt ccaattgcta
    37501 tgaaaggtgt aaaagtaaag ggttacagca ttgatagaat ttttataaat aatatgttga
    37561 tttatggatt aggtggatta atcgtcccgt ttttaggcat taaattaata gatatgattg
    37621 tacaattttt tgtgtaaaga ggaaggagcc tgtaccatga atacgataag aaatagtatt
    37681 tgtttaacga taataactat ggtattatgt ggatttttat tcccgttagc tatcacgtta
    37741 attggtcaaa tattttttta tcaacaagca aacggcagtt taattacgta tgacaatcgt
    37801 atagtaggat caaaattgat tggacagcac tggacagaga cacgatattt tcacgggaga
    37861 ccgagtgcag ttgattataa tatgaatcct gaaaaattat ataaaaacgg cgtatcatca
    37921 ggtggaagta atgaatcgaa tggaaacacc gaattaatag cacgcatgaa acatcatgtt
    37981 aaatttggga attcaaatgt aacaatagat gctgcaacaa gttctgggtc gggcttagat
    38041 ccgcatatta cggttgaaaa tgcattgaag caagcacctc gtattgctga tgctcgtcat
    38101 atatctactt caagagtggc cgacttgatt caacatcgta agcagcgtgg tgtgttaaca
    38161 aatgattatg tgaatgtgct tgaactaaat attgctcttg ataagatgaa agattgatta
    38221 tagatgaaag gcatcatgtt gataaaatta taacatggtg tctttttagc gattcaagca
    38281 ctttctgctt aactaaattt ttagatgtac ctttaggaag aataaattag catgaaatat
    38341 tccatatgct ataattagca acatataaaa ataaaatgaa atagattata aatccgtata
    38401 attttttatt tggggagagg ggtatatgtc agattatact aagttgtcta agagtcctaa
    38461 gtcagcattg ttgtattatt acattacgaa tggcttagaa tttatattga gtgtagctgt
    38521 gtatgtcatt ttttatttta tatggttaag atttgaatgg ccacattact tactttatat
    38581 tttgttggtt ttaggtgctc tgagtacatt aaaattatgt attaagcctt tatggcaata
    38641 tcattgtcgc ttttataaag ttgatcaact aagtgtacaa tatcgtacat catttttaat
    38701 atataaagaa gaaacgagtc gaattgaacg tttacaatac ttgtcgatta aatctaatcc
    38761 aattagtaaa gtgcttaatt tgtataaagt tggatttatg actgcaggtc atactattta
    38821 tttaccgatg atgtctcatg acgatgttaa aataatcgaa gcgagaacta tgtcgaattt
    38881 gagaggggtt gaaagtgatg tctgaacctc aaaaattaca tccgatttca tattttaatg
    38941 gcataatcaa tgctatcaaa caaaacatag tcgtcttttt tattttcatt atctttcaat
    39001 tgaaagattt tgattatacg aatcccgaat catatttatg gataggtatt gtatttgtat
    39061 tctttttaat atcatacatt tctcaaattg tcggaattat gaacacgcga tattggattg
    39121 aggataatta tttcatttta acaacaggta tttttaataa aaagcgtaaa gaattaaata
    39181 taaaacgtat tcagtccgtt gatatgacac aaggggtcgt caatcaaatt attggtggtg
    39241 tggatttaca aatcaaaaca ccaagtgatg gtattgtctt aagtgttatt tcgaaaaaac
    39301 aaggtgaata tcttgaacgc tatattgatc aattgcaaac agagttgaag acagaagtgg
    39361 cgcaaaagtc tagtgctgag aagaatgaca actgtaatca tgacattgaa ggtaatgtgg
    39421 aagacatcgc agaaggtcgc ttagatgaag tgcagaacac tgatagtatg cttaatcaga
    39481 aaaataaacg cattcctata tatcagatga attttaaaga atcactattt atggcaatga
    39541 ccagtggcgc cattggtgta acgttagcag ctttagtacc gatttacggg gctgtaagag
    39601 agttgatccc atggtcgaag ctaaatcatg aaattagtca atgggttcaa attgtaagta
    39661 tttcagtatt aatcatcatt ttactagtat taatagtggc atatattatt ggaacgatta
    39721 taacgatgat aagattttat ggcttttcgg tgatgcttga aaatgagcaa cttaaaattg
    39781 aatatggtct ctttaacaag aagaaagtaa ctgtaccaac gaaacgattg caagctgttg
    39841 ttgaacgtca gtcatatata cgaagattat ttggctacac agcaatacat tttatcatca
    39901 caagtgattt tgaaaataaa aaaatcgaag atgatgacaa tgattcaggt agtgtgccga
    39961 tattgccgtt tattaaacga gacaaagcat atcaaattat ccagaattta gtaccagatt
    40021 tatcttatca acaagttaat gaaggtatgc ctttgtcagg tttccacaga cactttttaa
    40081 tacctagtat tattgtcata gctatggcag gtattggctg ttatttttgg aatcaattat
    40141 tattgttatt tacgattata acaatagtca ttattggttt atttgtgatc aaaggatatc
    40201 tttatgtcaa acatgcgggt tttaaaatga cagaagaaca aattactatc cagaatttcg
    40261 agctattgaa acgaaatatt tattatttta aagcgcatca cattatagga tttgacactt
    40321 ggcaacatac attacttaaa cgcaacaatt taaataattt tagctttatt gttgctaaag
    40381 gtgtgggagc ccaatcgatt aacctaaaat acgcaagaag tgaagatgca aataaattga
    40441 aatcatggta tttaagaggt gaaaatgatg agggattata attttatgtc accacacgct
    40501 aaaaaagtga tgaggttaag tgctgtgctg ttttggatac ccatagcgct tgtactttta
    40561 atcgcattta atattttgaa ctggctattt tgggagatat tagataacaa tatatccatt
    40621 gttagtagtg tgattataat attacttgtt gctgttttca cgcttgtcat agcgccagta
    40681 tatcgatata agcactgcag atatgcattt gaaaaacacc atttgcgtgt acgaaacggc
    40741 attttatttt tggatgaaaa agtaatacca tattttcgta ttcaaaatat tgatatcgat
    40801 gttggtccta taatgagacg ctatcgatta gcaactttga ccctttatac agcgggtggt
    40861 caagctgaaa ttgaattaat agatagaagt gaagcacgta gattgaaacg atggttaaat
    40921 aacgagagac atttagaaaa caataaaaat gaggaataat caatgataca tggaattggt
    40981 gtagatttaa tcgaaatcga tcgaataaaa gtgttatata gtaagcagcc aaaattggtt
    41041 gagcggattt taactaaaaa tgaacagcac aaattcaaca atttcacaca tgagcaacgt
    41101 aaaattgaat ttttagctgg caggtttgct acaaaagaag cgttcagtaa agcattaggc
    41161 acaggcttag gaaaacatgt agcttttaac gatatagact gttacaacga cgaacttggc
    41221 aaaccaaaga ttgattacga agggttcatc gtacatgtta gtatctcaca cactgagcat
    41281 tatgcgatga gccaagttgt tttagaaaag tcagcatttt aaatttgtta tacaatgata
    41341 attaagatct agttttaaaa cgtgtgggaa ttacaggagg aagtaatatg tcggataaat
    41401 attatagatc tgcgtatatg aatgtagatt taaacgctgt tgcatcaaat ttcaaagtat
    41461 tcagtacatt gcatccaaat aaaacagtga tggctgtcgt taaagccaat gcctatggac
    41521 taggtagtgt taaagtagca cgtcatttaa tggaaaatgg cgccacattt tttgctgtag
    41581 caacgttaga tgaagcgata gaacttagaa tgcatgggat tactgctaaa attttagtct
    41641 taggtgtgtt accagctaaa gatattgata aagcgataca acaccgagtt gccttaacgg
    41701 ttccgtctaa acagtggttg aaagaagcaa ttaaaaacat ttctggtgag caagagaaaa
    41761 agttatggtt gcacattaaa ttagatacag gaatgggacg tttaggtatt aaagatacta
    41821 atacgtatca agaagtgatt gaaatcattc aacaatatga gcaacttgta tttgaaggcg
    41881 tgtttacaca ctttgcctgt gctgacgaac caggagatat gacaactgaa caatatcaac
    41941 gttttaaaga tatggtcaat gaagcaatta aacctgaata tatacattgt cagaactcag
    42001 caggttctct actaatggat tgccaattct gtaatgcaat aagaccagga atttcccttt
    42061 atggatatta tccatcagag tatgtacagc aaaaagttaa agtacacctt aaaccaagtg
    42121 tgcaattaat tgctaatgta gttcaaacaa agacgctaca agcgggtgag tctgtaagtt
    42181 atggtgcaac ttatacagct actgacccaa ctacaatagc attgttacct attggatatg
    42241 cagatggcta tttacgcata atgcaaggta gctttgtaaa tgtaaatggt catcaatgcg
    42301 aagttattgg tcgcgtatgt atggatcaga caattgttaa agtgccagat caagttaaag
    42361 ctggagattc ggtgatttta atagataatc atagagaaag tccacagtcg gtagaggtgg
    42421 tagctgaaaa gcaacatact attaattatg aagtgctttg taacttgtcg agacgtttgc
    42481 cgcgaatcta tcatgatggt gatcaacgtt ttgtaacaaa tgaattgtta aaataatatg
    42541 gtcagtgcat ataaaatttg ttatcattag agtaattaaa ggtcatttaa taacttttgg
    42601 aatcaattgg aggttctcat atgttatctt ttagtcaaaa tagaagtcat agcttagaac
    42661 aatctttaaa agaaggatat tcacaaatgg ctgatttaaa tctctcccta gcgaacgaag
    42721 cttttccgat agagtgtgaa gcatgcgatt gcaacgaaac atatttatct tctaattcaa
    42781 cgaatgaatg attagacgag gagatgttta tttagcagat ttatcaccag tacagggatc
    42841 tgaacaaggg ggagtcagac ctgtagtcat aattcaaaat gatactggta ataaatatag
    42901 tcctacagtt attgttgcgg caataactgg taggattaat aaagcgaaaa taccgacaca
    42961 tgtagagatt gaaaagaaaa agtataagtt ggataaagac tcagttatat tattagaaca
    43021 aattcgtaca cttgataaaa aacgattgaa agaaaaactg acgtacttat ccgatgataa
    43081 aatgaaagaa gtagataatg cactaatgat tagtttaggg ctgaatgcag tagctcacca
    43141 gaaaaattag gcgtctatta tatgtatttt tcagagataa ataaaatatt gatataaaag
    43201 acaataactt tataataatt ataactattt ctaaattctg tacgaagaat tttcttataa
    43261 acaaagattt tagcaaatac cagttatgat attcatattt tttattataa aaggatgtct
    43321 taagtttttt aggctttagg tattccatcc taaagttttt ttagcttaaa agtatcatct
    43381 acagcaaaat tgcaaacgac aaaattgata agtgcaatta aataaatgtt agtaagtgaa
    43441 tcataattat ccttgcttaa gcatttgctt tgtaagggaa gtgaggaggc aactaatcgt
    43501 ggaagaattt aagcaacatt ataagggttt aattgatgaa agtttaacgt gccaagataa
    43561 agtagaattg ataaaaaagt gtgagaaata cactgacgaa gtgattcgta aggacgtctt
    43621 gcctgaagac attgtcgata ttcacaaaaa ctatatattg acgttaaact taacgcgtga
    43681 agatgtgttc aagacattag atgtcttaca agaaatcgtt aaaggctttg gttatagtta
    43741 tcgagattat caaagattgg tagataaact tcaagttcac gataaagaga tagacttagc
    43801 ttctagctta caacaaacaa tgcttaaaac agatattcca caatttgata gtattcaaat
    43861 tggcgttatt tcagtggcgg cacaaaaagt aagtggagat tattttaatt taattgacca
    43921 taacgatggc acaatgagct ttgctgttgc agatgtcatt ggaaaaggta taccagctgc
    43981 tttagcaatg agtatgataa agtttggcat ggattcttat ggacactcac aattaccgag
    44041 tgatggttta aaacgtttaa atcgtgttgt tgaaaagaat attaatcaaa atatgttcgt
    44101 cacaatgttt tatggtttat atgaagaaat gaaccattta ttgtattgta gttcagctgg
    44161 tcatgagcct ggatatattt atcgcgctga aaaagaagaa ttcgaagaaa tttcagttag
    44221 aggtagagtg ttaggaatca gttcacaaac acgatatcaa caacaagaaa ttccaatata
    44281 ccttgatgat ttaattatca ttttaacgga tggtgtgact gaagctagaa atagtgaagg
    44341 tacctttata gataaacaaa aacttttaga atatattaaa aaacataaac atatgcaccc
    44401 acaagatatt gttcaaatta tctatgaagc aattttaaag cttcaaaacc caaataaaaa
    44461 agatgatatg actattttga ttataaaaag agtaaattaa tttaaaaaag aagattagaa
    44521 attatttcga tgggtatata ataatttgaa atataaatat ggtggataca gcgcttaaaa
    44581 tgaagataaa tatttttaat aagtaggagt gtaatgaaat gaatcttaat atagaaacaa
    44641 ccactcaaga taaattttac gaagttaaag tcggtggaga attagatgtt tatactgtgc
    44701 ctgaattaga agaggtttta acacctatga gacaagatgg aactcgtgat atttatgtta
    44761 atttagaaaa tgtgagttat atggattcga caggtttagg tttattcgta ggtacattaa
    44821 aagcattaaa ccaaaatgat aaagaactat acattttagg tgtgtcagat cgtatcggta
    44881 gactatttga aattactggt cttaaggatt taatgcatgt taatgaagga acggaggtcg
    44941 aataacatgc aatctaaaga agattttatc gaaatgcgcg tgccagcatc ggcagagtat
    45001 gtaagtttaa ttcgtttaac actttctggc gttttttcga gagctggtgc tacatatgat
    45061 gatattgaag atgccaagat tgcagttagt gaagctgtga caaatgcagt taaacatgca
    45121 tacaaagaaa ataacaatgt aggcattatt aacatatatt ttgaaatttt agaagataaa
    45181 attaaaattg ttatttctga taaaggtgac agttttgatt atgaaacaac taaatcaaaa
    45241 ataggtcctt acgataaaaa cgaaaatata gactttttac gcgaaggtgg cctaggttta
    45301 tttttaatcg aatctttaat ggatgaagtg acagtatata aagaatctgg tgtgacaatc
    45361 agtatgacta agtatataaa aaaagagcag gtgcgaaata atggcgaaag agtcgaaatc
    45421 agctaatgaa gtttcacctg agcaaattaa ccaatggatt aaagaacacc aagaaaataa
    45481 gaatacagat gcacaggata agttagttaa acattaccaa aaactaattg agtcattggc
    45541 atataaatat tctaaaggac aatcacatca cgaagattta gttcaagttg gtatggttgg
    45601 tttaataggt gccataaata gattcgatat gtcctttgaa cggaagtttg aagccttttt
    45661 agtacctact gtaatcggtg aaatcaaaag atatctacga gataaaactt ggagtgtaca
    45721 tgttccgaga cgtattaaag aaattgggcc aagaatcaaa aaagtgagcg atgaactaac
    45781 cgctgaatta gagcgttcac cttctatcag tgaaatagct gatcgattag aagtctcaga
    45841 agaagaagtg ttagaagcaa tggaaatggg acaaagttat aatgcgttaa gtgttgatca
    45901 ttccattgaa gctgataaag atggttcaac tgttacgcta ttagatatta tggggcaaca
    45961 agatgaccat tatgacttaa ctgaaaaacg tatgatttta gaaaaaatat tacctatatt
    46021 atctgatcgc gaacgagaaa tcatacaatg tacgtttatt gaaggattga gtcaaaaaga
    46081 gacaggtgag cgtatcggtt taagtcaaat gcatgtatca cgacttcaga gaacggcaat
    46141 taagaaatta caagaagcag cacatcaata gaatttgttt attaatgata cgttttataa
    46201 tgaaaaatcc atataattat cccttgatta ttaaattgaa atcgaggggt atttttaatt
    46261 taattaagat tttcgaatta atacattatt agtgtagttt aatgtgtatc cacataaatg
    46321 tcgcgatata gtattaataa tttaagtgaa gaagatatct aattgtcgtt ttaaataggt
    46381 gggttgctat tagaataaaa aagtagtctt agattatgaa atttagaaat gatggtgtgt
    46441 cattttcaat aatcttagtg cgttttaaaa tatagtatga cctaatcatt cgttttaaat
    46501 gttttggaag tgaaaattac attaagtatc ataccttaat agaagtattt tagaatatgt
    46561 taaaataaat gagtaaattt aagaaaaagt gtgggttaag taaatggaca atcaattgat
    46621 taattcaatc atagagaaat atcaatttag taaaaaacaa attgaagcag tattaacact
    46681 gctagaagaa aaaaatacag taccatttat tgcgaggtat cgaaaagagc aaactggtgg
    46741 actagatgaa gttcaaataa agcaaattga tgacgaatac caatatatgg tcaatttaca
    46801 aaaacgtaaa gaagaagtta tcaaaaatat agaacagcaa ggattactta ctgaggaatt
    46861 aaagaacgat attttaaaac agaacaaatt acaacgtgtt gaagacctat ataggccttt
    46921 taaacaaaag aaaaagacaa gggcaactga ggcgaaacgt aaagggttag agccattagc
    46981 gatatggatg aaggcacgta aacatgaagt ctcaattgaa gaaaaagcac aacaatttat
    47041 aaatgaagaa gtgcaatcgg ttgaagatgc tatcaaaggt gcacaagata ttattgcgga
    47101 acaaatttca gataatccta aatatagaac aaaaatttta aaagatatgt atcatcaagg
    47161 tgtgttaact acatctaaaa agaaaaatgc tgaagatgaa aaaggtattt ttgaaatgta
    47221 ctatgcatat agtgagccaa ttaaacgcat tgctaatcat agagttttag ctgttaatcg
    47281 tggtgaaaaa gagaaagtat tatctgtaaa gtttgaattc gatacgacat cagtagagga
    47341 tttcattgca cgtcaagaaa tcaatcataa taatgtaaat cgcagttata ttttagaggc
    47401 gattaaagat agcttgaaac gcttaattgt cccttcgata gagcgtgaaa tccatgctga
    47461 tttaactgaa aaagctgaaa atcatgcaat agatgttttt agtgaaaact taagaaatct
    47521 attactgcaa cctccaatga aaggtaaaca aatattaggc gtagatccag catttagaac
    47581 aggttgtaaa ttagcagtca ttaacccatt cggtactttt atagcaaaag gtgtgattta
    47641 tccgcatcca ccagtttcta aaaaagaggc agcagagaag gattttgtac aaatggttaa
    47701 agcgtatgat gtgcaattaa ttgcaattgg caatggtact gcaagtcgtg aaacagaaca
    47761 atttgttgca gatttaatta aaaagcatca gttgccagta caattcatca ttgtcaatga
    47821 agcgggcgct tcagtatact cagcatcaga aattgctaga gatgaatttc ctgattttca
    47881 agtggaagaa agaagtgccg tatcaattgg acgacgcgtt caagatccat taagtgaatt
    47941 agttaaaatc gatcctaaat ctattggtgt cggacaatac cagcatgatg ttaatcaaaa
    48001 agcactagaa aatgcattaa ctttcgttgt tgaaacagca gttaaccaag taggtgttga
    48061 tgtcaataca gcatcatcat cattattaca atatgtgtct ggtctaagtt cgcaaattgc
    48121 taaaaatata attgcatata gagaagaaaa tggcgcaata aagcataata aagagttaag
    48181 caagattaag cgtttgggtg ctaaaacttt tgagcagagt ataggatttt taagaatagt
    48241 agacggttca gagccattgg ataatacatc gattcatccc gaaagctata aagtaacgta
    48301 tcagttatta gataaactcg gatttggtgg caatgattta ggtagtgatg cattaaaagc
    48361 taaattaaat tcattggata tggatgaact ggcgattgaa ttacaagtcg gtgtacctac
    48421 attagaggat attattaaat ccttgaaagc acctaatcga gatccaaggg acgagtttga
    48481 aacaccaatc cttaaatccg atgtgctatc aattgaagat ttacaagaag gaatgaaatt
    48541 gagtggtaca gttagaaatg tagtagattt tggagcattt gtagatatag gtgttaaaca
    48601 agatggactt gtccatgttt cgaaactttc taagaagttc gtaaaaaatc caatggatat
    48661 tgtcagtgtt ggtgatatcg ttgacgtttg gatttacagt attgacaaaa ataaagacaa
    48721 agtatcatta acgatgattg atccacatga ataatgacaa acttcagcga atggttgaga
    48781 atctttcgga agaaaaattt ggacgtacgt ttcgacattg tgcatatttt aataaaaggt
    48841 tacgtacaac aggcggacgt tatcttttaa agtcccatga tattgaaatt aatccaaaac
    48901 aatatgagca ttatggagaa gatgctgttg taaaaattat actgcacgaa ttatgtcatt
    48961 atcacttgca tattgctggt aaaggttatc aacataaaga tcaagatttt aaacgattga
    49021 gccaacaggt tggagcgcct agattttgta acagcattga aagctatcaa caacgggcaa
    49081 attatgaata ttattgcact aaatgtcatg caaaatatat acgtatccgt aaagttgata
    49141 cgaatcgtat gcgttgcgga cattgcaatg gaaaattaag aatgaaaaga caattgaaat
    49201 aagtacaata caaatagagc taattcgtgt atatgtaaga ttatttatga gttgtatgga
    49261 atttatatag aatttatcag tgagttattt tgattggtaa acatagtaat tgaataagaa
    49321 gaaattgtgt ttataaaatg tttaaagccg agattgtcgt aatttaatac ttcgcaatct
    49381 cggcttttct acttatgata taggtaatgg cataaattgt gacttgatag gatgaaatta
    49441 aattgattag attaaaaatc ttgacgtatt ttagtaataa ataatatata aactttaccc
    49501 aattaaagct ttaaagtagc acaaaagata attatacatt tttaatgaca ttttgattga
    49561 act
//
LOCUS       DAMZLE010000007        45182 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMZLE010000007 DAMZLE010000000
VERSION     DAMZLE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 45182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.007"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..247
                     /locus_tag="POB92_000389"
     CDS             <1..247
                     /locus_tag="POB92_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6577915.1"
                     /translation="TIKSVHFTSLKRGPMGDAVIEGYINENKKADFVAYGSPEHNYQF
                     GGSLIKSKNLSTLLKPAHQTKTPDDIKKELDSKKNDR"
     gene            515..925
                     /locus_tag="POB92_000390"
     CDS             515..925
                     /locus_tag="POB92_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6577916.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYVSPENNFQ
                     FVGDLIKSERLSELLKPANQLKSPDDIKKELNKK"
     gene            952..1371
                     /locus_tag="POB92_000391"
     CDS             952..1371
                     /locus_tag="POB92_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6577917.1"
                     /translation="MNKKHVFVIIGVILCICIVVTAIHFKIKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTNFETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAHQLKSPDDIKKELNKKKSH"
     gene            1389..1808
                     /locus_tag="POB92_000392"
     CDS             1389..1808
                     /locus_tag="POB92_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6577918.1"
                     /translation="MSKKHGFIIIGVILCICIVASVIYLKMKYDEKEKQKALYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKADFVAYGSPEHNYQ
                     FGGTMIESERLSELLKPANQLKSPEKIKEELDKKEGH"
     gene            2260..2682
                     /locus_tag="POB92_000393"
     CDS             2260..2682
                     /locus_tag="POB92_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI6577919.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(2794..4332)
                     /locus_tag="POB92_000394"
     CDS             complement(2794..4332)
                     /locus_tag="POB92_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDI6577920.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WFISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            4567..5385
                     /locus_tag="POB92_000395"
     CDS             4567..5385
                     /locus_tag="POB92_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6577921.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            5648..6010
                     /locus_tag="POB92_000396"
     CDS             5648..6010
                     /locus_tag="POB92_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577922.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLAILKYLNTVSIENISHSELLNLIENTIPEDRDINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLK"
     gene            6885..7706
                     /gene="cntK"
                     /locus_tag="POB92_000397"
     CDS             6885..7706
                     /gene="cntK"
                     /locus_tag="POB92_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDI6577923.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            7717..8535
                     /gene="cntL"
                     /locus_tag="POB92_000398"
     CDS             7717..8535
                     /gene="cntL"
                     /locus_tag="POB92_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDI6577924.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            8528..9832
                     /gene="cntM"
                     /locus_tag="POB92_000399"
     CDS             8528..9832
                     /gene="cntM"
                     /locus_tag="POB92_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDI6577925.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVNGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLSQ"
     gene            9972..11570
                     /gene="cntA"
                     /locus_tag="POB92_000400"
     CDS             9972..11570
                     /gene="cntA"
                     /locus_tag="POB92_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDI6577926.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSGAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            11583..12518
                     /locus_tag="POB92_000401"
     CDS             11583..12518
                     /locus_tag="POB92_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577927.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAAIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            12515..13384
                     /locus_tag="POB92_000402"
     CDS             12515..13384
                     /locus_tag="POB92_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577928.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            13381..14196
                     /locus_tag="POB92_000403"
     CDS             13381..14196
                     /locus_tag="POB92_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577929.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNLERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDSKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            14189..14938
                     /locus_tag="POB92_000404"
     CDS             14189..14938
                     /locus_tag="POB92_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577930.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            14950..16143
                     /locus_tag="POB92_000405"
     CDS             14950..16143
                     /locus_tag="POB92_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6577931.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PASSL"
     gene            complement(16505..17254)
                     /locus_tag="POB92_000406"
     CDS             complement(16505..17254)
                     /locus_tag="POB92_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577932.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDAFTVFDIYPLIKGYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            17450..19027
                     /locus_tag="POB92_000407"
     CDS             17450..19027
                     /locus_tag="POB92_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDI6577933.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGERLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(19195..19650)
                     /locus_tag="POB92_000408"
     CDS             complement(19195..19650)
                     /locus_tag="POB92_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDI6577934.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            20009..20266
                     /locus_tag="POB92_000409"
     CDS             20009..20266
                     /locus_tag="POB92_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6577935.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            20266..20532
                     /locus_tag="POB92_000410"
     CDS             20266..20532
                     /locus_tag="POB92_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDI6577936.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            20888..21964
                     /locus_tag="POB92_000411"
     CDS             20888..21964
                     /locus_tag="POB92_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6577937.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            22212..22874
                     /locus_tag="POB92_000412"
     CDS             22212..22874
                     /locus_tag="POB92_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6577938.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVHNKEKIENIIFKLVEQDVAIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            22867..23643
                     /gene="fetB"
                     /locus_tag="POB92_000413"
     CDS             22867..23643
                     /gene="fetB"
                     /locus_tag="POB92_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDI6577939.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAIVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            24039..25226
                     /locus_tag="POB92_000414"
     CDS             24039..25226
                     /locus_tag="POB92_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6577940.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(25288..26640)
                     /locus_tag="POB92_000415"
     CDS             complement(25288..26640)
                     /locus_tag="POB92_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDI6577941.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLKSARNKAQYFQKLMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(26947..28584)
                     /locus_tag="POB92_000416"
     CDS             complement(26947..28584)
                     /locus_tag="POB92_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6577942.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIVVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            28764..29363
                     /locus_tag="POB92_000417"
     CDS             28764..29363
                     /locus_tag="POB92_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDI6577943.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARSEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            29367..29507
                     /locus_tag="POB92_000418"
     CDS             29367..29507
                     /locus_tag="POB92_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577944.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCACHGQHFDVGIPLQA
                     WE"
     gene            29587..29706
                     /locus_tag="POB92_000419"
     CDS             29587..29706
                     /locus_tag="POB92_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577945.1"
                     /translation="MDVCPPVTMDACALLQKFEFCNNISHFRHFFAIKQPFER"
     gene            30033..31259
                     /locus_tag="POB92_000420"
     CDS             30033..31259
                     /locus_tag="POB92_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6577946.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRSIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDSDNHLLGFLDIEDINQGIRGHKTLRDMMQQHIYTVQIDSKLQDSVRTI
                     LKRNIRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQAEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            31256..31891
                     /locus_tag="POB92_000421"
     CDS             31256..31891
                     /locus_tag="POB92_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577947.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            31908..32849
                     /locus_tag="POB92_000422"
     CDS             31908..32849
                     /locus_tag="POB92_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6577948.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            32849..33544
                     /locus_tag="POB92_000423"
     CDS             32849..33544
                     /locus_tag="POB92_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577949.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            33838..35238
                     /locus_tag="POB92_000424"
     CDS             33838..35238
                     /locus_tag="POB92_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6577950.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNTLLAIV
                     AFLVILLLVPKNQTNL"
     gene            35464..36399
                     /locus_tag="POB92_000425"
     CDS             35464..36399
                     /locus_tag="POB92_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI6577951.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     DAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            36721..37572
                     /locus_tag="POB92_000426"
     CDS             36721..37572
                     /locus_tag="POB92_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6577952.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(37664..39160)
                     /locus_tag="POB92_000427"
     CDS             complement(37664..39160)
                     /locus_tag="POB92_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDI6577953.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIHITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTQYITDHW
                     LKPLQMKHTTFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            39392..41221
                     /locus_tag="POB92_000428"
     CDS             39392..41221
                     /locus_tag="POB92_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6577954.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(41361..43439)
                     /locus_tag="POB92_000429"
     CDS             complement(41361..43439)
                     /locus_tag="POB92_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6577955.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(43577..44986)
                     /locus_tag="POB92_000430"
     CDS             complement(43577..44986)
                     /locus_tag="POB92_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6577956.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT        15410 a         6635 c         8564 g        14573 t
ORIGIN      
        1 cacgattaaa tctgtgcatt tcacaagttt aaaaagagga cctatgggcg atgccgtaat
       61 tgaaggttac atcaatgaaa acaaaaaagc tgattttgtt gcatatgggt ctccagaaca
      121 taattatcaa tttggtggaa gtttaatcaa aagtaaaaat ttaagcacgt tattaaaacc
      181 agcacatcaa accaaaacgc ctgatgatat aaaaaaagaa ctagattcta aaaagaacga
      241 ccgctaaaac tatgcggtcg ttttaatttt gttttcaagc taatcacact tacacaaaaa
      301 aataaaaact tgaattttta gtacaacatt ccactgtatt tatctgttaa ccactttata
      361 cccgcaatat ttcgttgatc ttatataaac acttttggct gcttttcctt gacctagatt
      421 aaaattatag attttctttg taagagtgtg ttttgtagta tcataaatat gtaaactgta
      481 taaaatccca ccacatttta ggagctggtg atagatgtcg aaaaagaaaa ttttaatatt
      541 aactagtatt atgttaatca tattaattag tgttgcaagt atctatttta aaatgaaata
      601 tgacgaaaaa gaaaaacaaa aagcaattta ttataaagaa caacaagcgc gcattacact
      661 ttatctcaag cataacacta aagaaccgaa cacgattaaa tctgtgcatt tcacaaaatt
      721 ggaaacaagt cctatgggaa gtgctgtgat tgaaggttac ataaatgaaa acaaaaaaga
      781 tgattttgtt gcctatgtat cacctgaaaa taactttcaa tttgtaggtg atttaattaa
      841 aagtgaaaga ttaagtgagt tactaaaacc agcaaatcaa ttaaaatcac cagatgatat
      901 aaaaaaagaa ctaaataaaa agtagagtca ctaaagttag gagttacttt aatgaacaaa
      961 aaacatgttt ttgtaattat tggtgtcatt ttatgtatat gtatagttgt tacggccata
     1021 cattttaaaa ttaaatatga tgaaaaagaa aaacaaaaag caatatacta taaagaacaa
     1081 caagagcgca ttacacttta tctcaagcat aataccaagg agccaaacac aatcaaaact
     1141 gtacatttca ctaactttga aacaagtcct atgggaagtg ctgtgattga aggctacatc
     1201 aatgaaaaca aaaaagataa ttttactgct tatgctacac ctgaacataa ttatcagttt
     1261 ggtggtgcta tgatagaaag tgaaagatta agtgagttac taaaaccagc gcatcaatta
     1321 aaatcaccag atgatataaa aaaagaacta aataaaaaga agagtcacta aagttaggag
     1381 ttgctttaat gtccaaaaaa catggtttta taattattgg tgtcatttta tgtatatgta
     1441 tagttgcatc cgtcatttat ttaaaaatga aatatgacga aaaagaaaaa caaaaagcac
     1501 tttactacaa agaacaacag gagcgtatta cactctatct caagcataat accaaagaac
     1561 caaacacgat taaatctgtg catttcacaa gtttaaaaag aggacctatg ggcgatgccg
     1621 taattgaagg ttacatcaat gaaaacaaaa aagctgattt tgttgcatat gggtctccag
     1681 aacataatta tcagtttggt ggtactatga tagaaagtga aagattaagt gagttactaa
     1741 aaccagcaaa tcaattaaaa tctcctgaaa aaattaaaga agaattagac aaaaaagaag
     1801 gccactaggg tcttctttat ttttgattta atcttccaat aatctatgtc attgctatcg
     1861 aaggtgtttc gcaattaata tcaatcactt catcatcacc aatacttccc cagttttgta
     1921 cagtacatta acacaaacga accacgttaa tttaaatgga ataatagttt ggccattata
     1981 agaacaatat atatcgaata acaatgtcga tgacgtggtt tattgacatt atcaaatttt
     2041 ctcaaatgtg tgtgattttt tataaaaaag ggtagagcaa ggtataatat cctttcaatc
     2101 tgttttcata ttttatatat tttttaaata taagcgctaa atgttttaac taaaacatag
     2161 attgacaaga tgttatacag aatttcaaat tctatccaat attgttcgaa gtgtagtatc
     2221 actggattgg tattaaacaa tgtaaaggag agattgcaaa tgccgtataa ttacaagaaa
     2281 caaaatggag agttaatgtc tgtaatgagc caaggtgaaa agtttattca tcaatcaccc
     2341 gttaatgatg aacttagtgc attgattaag ttattaattt caaaaattaa cggttgtcat
     2401 tattgtgttg atatccataa aaaagaatta aaggaattgg gtgtaacaca aatgaaaatt
     2461 gatgaagtct tgagttttag acatttagat ttatttactg atcaagaaaa agtgacgctt
     2521 gaatttgcag aaatgttaaa ttcaatcaaa gactttaaga agtttgaaat tattgaccgg
     2581 ctaaaatcat tttatgatga agaacaaatt attgatcttg tctttgttgt aaaccaaatt
     2641 aacggttgga acaggctaaa tattattagt gataaactat aattcttcat ataaatacag
     2701 agtttcatct cgaacgctat atcataacaa atcatgccac tatacaggtc aaatcttgta
     2761 tagtggcatt ttaatttatc cctttgaata ctgttattta acgaatatcg gtccacctgg
     2821 tccaacaggg atacctaata ggaaccaaat aatgacaaat actgtccaca caatacttag
     2881 cgcgattgaa tacggcatta aactagaaag taaggctccg agtttcatgc gtttatcgta
     2941 tttttgtgca taagttaata ataaaggtaa gtacggcatc atcggtgtaa ttggattggt
     3001 aattgaatct cctacacggt aaatgacttg tgtgaatgcg ggatgaaagc cgataaggat
     3061 taacattggt acgaatatcg gtcctaaaat accccattta gccgatgcgc ttccgattaa
     3121 catgttgacc attgcactca gtacaataat acctagtatc aatacaatgc cgttttgatg
     3181 ttctaataat ttggcacctt taacagcagc gataattcct aaattactcc actttaaata
     3241 cgcaagtagc tgtgctgcaa aaaacacaat aacgataaat gttcccattg atcctacagc
     3301 atcgccgaac attttaccta agtcttttgt atttttaatt tctttgctta aaatcccata
     3361 aactaatcca ggtactaaaa atacaacaag aataattaat ccgacaccgt taattaatgg
     3421 cgcatcgtct agtaagctgc ctgttttagc atttcttaaa aagctatgtt caggaatggc
     3481 tgtaataatt aataaaataa ttgtgactat gaaactgata tttgcccatt ttaaagcatg
     3541 agcttctttg tcagtaatat gtgaagacgt ttcttcatga tcatcatgca ttaaacgatc
     3601 atcatattga cctaacctag gaataatcag tttagttgta actagcaata tcgtaggaag
     3661 taatacaacg acactcgctg cgataaagta ccagttcata gcaacgtttg ttttaataga
     3721 atctgaaacg atacgtgttg ctggttctgt aaatgaatag accaaagcat cttgcatacc
     3781 aacaactata tttgcggcaa agccacctac agcggaagca tatgccatcg ttagtccagc
     3841 gataggatga tagccaattt taataaaaag catcgctgcg agcggcggca agataattgt
     3901 cgcagcatcg ccggctgtac tacctaaaat accaattaat ataatagtcg gtaaaattaa
     3961 gaaacgtggt gcgcgattca caacagaaat cattaacttg tcgaagtatc ctgttttctc
     4021 tgcaacacca ataccaatca tcactgctag tactaagcct aatgctggga actctgagaa
     4081 atttttaatc gtatcattca ttatcatcgt aaatccatca tggctaatta tatttttaat
     4141 ataaatggtt tgatgcgtac ctggatgctt aacagataca ttaaataatg agataaacca
     4201 cgtcataatg gctaagccca cacacattaa aaagaataaa acgctaggat ctggcaattt
     4261 atttccaatt ttttcaacac tattcaagaa acgattgacg atagacccct tttgttgatg
     4321 ttttgatgtc atcaatgatt cccccctttg ttaaatattt aaagtgtaac aaaaaatact
     4381 ctcaaaagta acaattttca gaaaatgaaa aatctaatat tgttaaatat tttgagttat
     4441 tcaatagaaa ttgtatagca gagagtagat aagactgcct ggagacttat ctattaggtt
     4501 tatgaagcat tgaacagtgg aaaataagga ctgtaagttt aagatatgtt gtataggagt
     4561 gactgaatga aacgtttgga aaataaagtt gcagtcgtaa caggagcaag tacaggtatc
     4621 ggacaagctt ctgcaatcgc tttagctcaa gaaggtgcgt atgtattggc ggtagacata
     4681 gctgaagcgg tatcggagac tgtcgataaa attaaaagta atggtgacaa agcaaaggct
     4741 tatattgtgg atatagcaag cgaacaacaa attgacaatt ttgcatcaga aattagagaa
     4801 cagattgggc atgttgatgt gttatttaat aatgccggtg tggataatgc ggctggtaga
     4861 attcatgagt atccaacaga tgtatatgac aagattatga atgtagatat gcgtggaaca
     4921 tttttgatga cgaaaatgat gttaccttta atgatgacta aaggtggctc tattgttaac
     4981 acatcttcat tttcaggaca ggcagcagat ttgtatcgct caggatataa tgctgcgaaa
     5041 ggggcagtga ttaattttac gaaatccatt gcaatcgaat atggtagaga tggcattcga
     5101 gctaatgcga ttgcaccagg tacgattgaa accccgttag tagataaact gacaggtacg
     5161 agtgaagata aggaaggcaa agcattcaga gaaaatcaga aatggatgac accgctggga
     5221 cgtttaggta aaccagaaga agtaggcaag ctcgttgtct ttttagcatc tgatgagagt
     5281 tcatttatta caggtgaaac aattcgtatt gacggtggtg ttatggctta cacatggcct
     5341 ggcgagatgt tgagtgatga ttcgtggaag cggacattgg aataattatt taaaatcgat
     5401 atccagtgga aaagaatttg gcatgaagta ggcaatgtat gcatattgtc gcaatgaaag
     5461 acatttaaag ccgagattaa ttaatctttg aaaatcacat attgttcatt tgaagtgtat
     5521 tgcttaaagc ttgttttaac gtattgtagg tcgtgcatct aatttatcag aatctcgagt
     5581 tcaaactttt ggagtatctt taaaataagc tgtattttgt ctattctaat aaattaagga
     5641 gaattttatg ttaaaagaaa aagaaagttt tagattgcta tatcaagcta taagagagat
     5701 tgcagataaa attggagata atcagttaga aactaattcc gttagtttat tattattgga
     5761 ctttgatttt gaacatgaag tatttgatga attgtatctt gcgattttaa aatatttaaa
     5821 tacagtaagt atagagaaca taagtcatag tgagcttttg aatttaatag aaaatacgat
     5881 tccagaagat cgagatatta acacgtttgt aaaaaataaa ataataattg gctttgctaa
     5941 taattatttc cctgaattac aagtattagc aaacgaaata aaatctgata tggctagttc
     6001 attaaaataa tgatattttt atttgaattt ttaaagcttt gtacgaaatt gtacaaagct
     6061 ttttttggtg cgcattgcat gggcaacaac ttgacgatga aaattcgtta caggattggt
     6121 gataggaaat gttagcgaaa gacaagggga tccattgtag attaacaaaa ggacgtttcc
     6181 acaagtgtgg gttattctca ctaaagcaat acgcagagac aacttacgta aaattttgaa
     6241 ctgacgagaa cggaacttct attcaattat tgataaaaat tttcaaaaag acttgaatgt
     6301 gctgagaata cgatgtttat ggaaggatta tcaaaatata aatgtgcatt catttacaac
     6361 ctttattgac aatgattctc aactaatata gtatataatc aaatcgtaat agttacgatt
     6421 tgttttctgc aacttttttg aagttttagt tgaggtgaaa acagtaaaag catctaagtg
     6481 aatgtagtta acggacaact gcattcgctt gtagagccac aagaagcaac tttaaataag
     6541 gtttacggtt gcatgttgat acaacaaccg attactaagt catgctttcc actttgcggg
     6601 ttagcatgac ttacctaata gatgggaaac tattaggttc atcttctatt aaaaattaca
     6661 ggtttagagg aatacagttg cttgcttcgc aacaactgca taagagccat ggttttcgct
     6721 tttgcgaatt agcatgactt acctactaga tagagctatt aggttcatct tctaaaaaat
     6781 tacaggttta gaggaataca gttgtttgct tcgcaacaac tgcataagag cctctagtaa
     6841 ttaaaattac agaggctcta aaaatacatc taaaggagtg tcgtatgaat cggcaggtta
     6901 tagaattttc taagtataat ccttcgggga atatgacgat acttgttcat tcaaaacatg
     6961 atgctagtga atatgcatct atcgccaatc agttgatggc cgcaacacat gtatgttgtg
     7021 aacaggtagg ctttatagaa tcaacacaaa atgatgatgg caatgatttt cacttagtta
     7081 tgagcggtaa tgaattttgc ggtaatgcga cgatgtcata tatacatcat ttgcaagaaa
     7141 gtcatttgct taaagaccaa cagtttaagg tgaaggtgtc tggctgttcg gatttagtgc
     7201 aatgcgcaat tcatgattgc caatactatg aagttcaaat gccacaagcc catcgtgttg
     7261 tgccaacaac aattaatatg ggtaatcatt catggaaagc aatagaaatt atttatgaaa
     7321 catatgtaca ttatgtgatt ccagttaaac aagtaacaac agaaattcaa catttggttg
     7381 aagcgtttgt gcgtgagcaa caatggagtc acaaatataa aacagtaggt atgatgcttt
     7441 ttgatgaaca acgtcaattt ttacagccat taatctatat accagaaatt caaagtttaa
     7501 tttgggaaaa tagctgtggt tctggtacag catcaattgg ggtttttaat aattatcaac
     7561 gtaatgacgc atgcaaagat tttacagtac atcagccagg gggcagtatt ttagtgacat
     7621 caaagcgatg tcatcaattg ggatatcaaa cttcaattaa aggacaggtt acaactgtag
     7681 ctacaggaaa agcatatata gaataaggag cctacaatga ataactttaa taatgaaatc
     7741 aaattgatat tacaacaata tttagaaaag tttgaagcgc attacgagcg tgtattacaa
     7801 gacgatcaat atatcgaagc attagaaaca ttgatggatg actatagtga atttatttta
     7861 aatcctattt atgaacaaca atttaatgct tggcgtgacg ttgaagaaaa agcacaatta
     7921 ataaaatcac tgcaatatat tacagcgcag tgtgttaaac aagtggaagt cattagagcg
     7981 agacgtctat tagacggaca ggcgtctacc acaggttact ttgacaatat agaacattgt
     8041 attgatgaag agtttggaca atgtagtata gctagcaatg acaaattatt gttagttggt
     8101 tcaggtgcat atccaatgac gttaattcaa gtagcaaaag aaacaggtgc ttcagttatc
     8161 ggtattgata ttgatccaca agccgttgac ctagggcgca gaatcgttaa cgtcttagca
     8221 ccaaatgaag atataacaat tacggatcaa aaggtatctg aacttaaaga tatcaaagat
     8281 gtgacgcata tcatattcag ctcgacaatt cctttaaagt acagcatttt agaagaatta
     8341 tatgatttaa caaatgaaaa tgtcgtagtt gcaatgcgct ttggtgatgg catcaaagca
     8401 atatttaatt atccgtcaca agaaacagcg gaagataagt ggcaatgtgt gaataaacat
     8461 atgagaccac agcaaatttt tgatatagca ctttataaaa aagcagctat aaaggtaggt
     8521 attacggatg tctaaattat taatgatagg cactggtccg gtcgcaatcc aattagcgaa
     8581 tatttgctat ttaaaatcag attatgagat tgatatggtt ggacgtgcct caacatcaga
     8641 aaaatcaaaa cgcttatatc aagcgtataa aaaagagaaa caatttgaag tcaaaataca
     8701 aaacgaggcg catcaacatc tggaaggtaa gtttgaaatt aatcgtttgt ataaagacgt
     8761 taaaaacgtt aatggtgaat acgaaacggt tgtcatggca tgcacagcag atgcttatta
     8821 tgacacacta cagcaattgt cgttagaaac tttgcaaagt gtcaaacatg tcattttaat
     8881 atcaccgaca tttggttcgc aaatgattgt cgaacaattt atgtctaaat ttaataaaga
     8941 tatcgaagtg atttcattct caacttatct tggcgataca cgtattgttg ataaagaagc
     9001 gcctaatcat gtgttgacaa caggtgtaaa aaagaaattg tacatgggat cgacacattc
     9061 aaactcaaca atgtgtcaac gaatctctgc tttagctgag caattgaaaa ttcaattaga
     9121 agtcgttgaa tcgccactgc acgctgaaac gcgaaacagt tcgctttatg tgcacccacc
     9181 actatttatg aatgactttt cattgaaagc cattttcgaa gggacggatg taccggttta
     9241 tgtgtataag ttatttcctg aaggaccgat aacgatgaca ctaatccgtg aaatgcgttt
     9301 aatgtggaag gaaatgatgg ctattttaca agcatttaga gtgccgtcag tcaacctgct
     9361 tcaatttatg gtgaaggaaa attatccagt acgtcctgaa actttggatg aaggtgatat
     9421 tgagcatttc gaaatcttgc cagatatctt acaagaatat ctgctttatg taagatatac
     9481 cgcaatcctc attgatccat tttcacagcc agacgaaaac ggacattact ttgatttttc
     9541 agctgtacca tttaagcaag tctataaaaa tgaacaggat gttgttcaaa ttccaagaat
     9601 gccaagtgaa gattattaca gaacggcgat gattcagcat attgggaaaa tgctaggtat
     9661 caaaacgcca atgattgatc agttcctaac tcgctatgaa gcaagttgcc aggcgtacaa
     9721 ggatatgcat caagatcaac acttatcttc tcaatttaat acaaatctat ttgaaggaga
     9781 taaagcactc gtcacaaaat ttttggaaat caatagaacg ctttcacaat aagggtttga
     9841 agttttataa tagaaaaaaa ttattgaatt atgtttgaca tttacataaa aataagcaaa
     9901 taattgagaa aaataatcat tacgatttga ttaagtaatg caacttatca atttagaaag
     9961 aggaaaagca aatgagaaaa ctaactaaaa tgagtgcaat gttacttgca tcagggctaa
    10021 ttttaactgg ttgtggcggt aataaaggtt tagaggagaa aaaagaaaac aagcaattaa
    10081 cgtatacgac ggttaaagat atcggtgata tgaatccgca tgtttacggt ggatcgatgt
    10141 ctgctgaaag tatgatatac gagccgcttg tacgtaacac gaaagatgga attaagcctt
    10201 tactagctaa aaaatgggat gtgtctgaag atgggaagac atatacattc catttgagag
    10261 atgacgttaa attccatgat ggtacaccat ttgatgctga cgcagttaag aaaaatattg
    10321 atgcggttca acaaaataaa aaattgcatt cttggttaaa gatttcaaca ttaattgaca
    10381 atgttaaagt taaagataag tacacggttg aattgaattt gaaagaagca tatcaacctg
    10441 cattggctga attagcgatg cctcgtccat atgtatttgt gtctccaaaa gactttaaaa
    10501 acggtacaac aaaagatggc gttaaaaagt tcgatggtac tggtccattt aaattaggtg
    10561 aacataaaaa agatgagtct gcagacttta acaaaaatga tcaatactgg ggcgaaaagt
    10621 ctaaacttaa caaagtacaa gcaaaagtaa tgcctgctgg tgaaacagca ttcctatcaa
    10681 tgaaaaaagg tgaaacgaac tttgccttca cagatgatag aggtacggat agcttagaca
    10741 aagactcttt aaaacaattg aaagatacag gtgactatca agttaagcgt agtcaaccta
    10801 tgaatacgaa aatgttagtt gtcaattctg gtaaaaaaga taacgctgtg agtgacaaaa
    10861 cagtcagaca agcgattggt catatggtaa acagagataa aattgccaaa gaaattttag
    10921 atggtcaaga aaaaccagca actcaattat ttgcgaaaaa tgtaacagat attaatttcg
    10981 atatgccaac acgtaagtat gaccttaaaa aagcagaatc attattagat gaagctggtt
    11041 ggaagaaagg taaagacagc gatgttcgtc aaaaagatgg taaaaacctt gaaatggcaa
    11101 tgtactatga caaaggttct tcaagtcaaa aagaacaagc agaatactta caagcagaat
    11161 ttaagaaaat gggtattaag ttaaacatca atggcgaaac atcagataaa attgctgaac
    11221 gtcgtacttc tggtgattat gacttaatgt tcaaccaaac ttggggatta ttgtacgatc
    11281 cacaaagtac tattgcagca tttaaagcga aaaatggtta tgaaagtgca acatcaggca
    11341 ttgagaacaa agataaaata tacaacagca ttgatgacgc atttaaaatc caaaacggta
    11401 aagagcgttc aggcgcttat aaaaacattt tgaaacaaat tgatgatgaa ggtatcttta
    11461 tccctatttc acacggtagt atgacagttg ttgcgccgaa agatttagaa aaagtatcat
    11521 tcacacaatc acagtatgaa ttaccattca atgaaatgca gtataaataa aggagcaatt
    11581 agatgttcaa atttatctta aaacgtattg cgctcatgtt tccattagtg attgtagtaa
    11641 gttttatgac atttctattg acgtatatta caaatgaaaa tccagctgtg acaattttac
    11701 atgcacaagg gacgccaaat gtaacaccag agttgattgc agaaacgaat gagaagtacg
    11761 gtttcaatga tccattatta attcaatata aaaattggtt acttgaagcg atgcaattta
    11821 attttggtac aagctacatt acaggtgatc ctgttgctga acgcattggt ccagcattta
    11881 tgaatacatt gaaattaaca ataatttcaa gtgttatggt gatgattaca tcaattattt
    11941 taggtgtagt tagtgcatta aaaagaggaa agttcactga tcgtgcgata cgttcagtgg
    12001 ctttctttct aactgcatta ccatcatatt ggatagcttc aatacttatt atttacgttt
    12061 cagtgaagtt aaacatattg ccgacttctg gattaacagg tccagaaagt tacatattgc
    12121 cagtgatcgt tattacgatt gcctatgctg ctatttactt tagaaatgtt agacgctcga
    12181 tggtggaaca attaaatgaa gattatgtac tttatttaag agcaagcggt gtgaaatcta
    12241 tcacattaat gttgcatgtg ttgcgtaatg ctttacaagt tgcggtatca atcttttgta
    12301 tgtctatacc aatgataatg ggtggactag ttgttatcga gtatatcttt gcatggcctg
    12361 gactaggtca attaagttta aaagcaatac ttgaacacga ttttccagtt attcaagcat
    12421 atgtattaat tgtagcggta ttatttattg tatttaatac attagcagat atcattaatg
    12481 cgctattaaa tccaagatta agggagggcg cacgatgata attttaaaac gattattaca
    12541 agataaaggt gcagtaattg ctttaggcat tattgtatta tatgtctttt taggattagc
    12601 agcaccactt gtgacatttt atgatcctaa ccatatcgat acagcaaaca aatttgctgg
    12661 catgagtttt caacatctac taggtactga ccatttaggt agagatattt taactaggtt
    12721 aatttatgcg attagaccaa gtttgttata tgtctttgtt gcgctatttg tttctgtact
    12781 tattggatct attttaggat tcttatcagg atatttccaa gggtttgttg acgccttaat
    12841 catgcgtgcg tgtgatgtta tgttggcatt cccaagttat gttgtaacgt tagcattaat
    12901 tgcattgttt ggtatgggtg ccgaaaatat tatcatggca tttattttga cgcgttgggc
    12961 atggttctgt cgtgttatac gtacaagtgt tatgcagtac actgcttctg accatgtaag
    13021 atttgctaaa acaatcggta tgaatgatat gaaaattatt cacaaacata ttatgccgtt
    13081 aacattagca gatattgcta tcatctctag tagttcgatg tgttcaatga tcttgcaaat
    13141 atctggcttt tcatttttag gattaggtgt caaagcgcct actgcagagt ggggcatgat
    13201 gcttaacgaa gctagaaaag tgatgtttac acatcctgaa atgatgtttg cgccaggtat
    13261 tgccatagtg attatagtga tggcatttaa cttcttatcc gatgctttac aaattgctat
    13321 tgatccccgc atctcttcta aagataaact tcgttctgtg aaaaaaggag tggtgcaatc
    13381 atgacattgt taacagttaa acatttgacg attacagata cctggacaga tcaaccactc
    13441 gtgagtgatg tgaattttac attaactaag ggtgaaactt taggcgttat tggagaaagt
    13501 ggtagcggta aatcaatcac ttgtaaatcg attattggtt tgaatctcga acgactcggg
    13561 gtgacaggtg aaattatctt tgatggtaca tcaatgttgt cattatctga atcgcaattg
    13621 aaaaagtacc gtggtaaaga cattgcgatg gtcatgcaac aaggtagtcg tgcctttgac
    13681 ccatcaacta ctgtcggtaa acaaatgttt gagactatga aagtacatac gtcaatgtct
    13741 acacaagaaa ttgaaaagac attgattgaa tatatggatt atttaagttt gaaagattct
    13801 aaacgtatat taaaatcata cccttacatg ttatcaggag gaatgttaca gcgattgatg
    13861 attgctttag cgttagcttt gaaaccaaag ttaatcattg ctgatgagcc gacaacggct
    13921 ttagatacaa ttacacaata tgatgtactg gaagcattta tagatattaa aaaacacttt
    13981 gactgtgcga tgattttcat ttcacatgat ttaacggtta ttaacaagat tgcagaccgt
    14041 gttgttgtga tgaaaaatgg tcagcttatt gaacaaggga cacgtgaatc agtcttgcat
    14101 catccagaac atgtttatac gaagtattta ttatcaacga agaagaagat taatgatcat
    14161 tttaaacatg tgatgagggg tgatgtacat gattaaaatt aaagatgttg aaaagtcata
    14221 tcaaagcgca catgttttta agcgtcgtcg aacacctatc gtgaaaggtg tgtcatttga
    14281 gtgtccaatc ggtgcgacga ttgcgattat cggagaaagt ggtagcggta aatcgacatt
    14341 gagtcgtatg atattaggta ttgagaaacc ggataaaggt tgtgtaacct taaatgatca
    14401 accgatgcat aagaagaaag tgagacgtca tcaaattggt gctgtatttc aagattatac
    14461 gtcatcatta catccatttc agactgttag agaaatctta tttgaagtga tgtgtcaatg
    14521 tgatggacaa cctaaagatg ttatggaagt tcaagcaatt acattgttgg aagaagtcgg
    14581 tctatctaag gcatacatgg ataaatatcc taatatgtta tcaggtggag aggcgcaacg
    14641 tgttgcgatt gcgcgtgcaa tatgtattaa ccctaaatat attttgtttg atgaagccat
    14701 tagttcactc gacatgtcaa ttcaaacaca aatattagat ttattgattc atttacgtga
    14761 aacgcgtcag ttgagttata tttttatcac acatgatatt caagctgcca cgtatttatg
    14821 tgatcaatta attattttta aaaacggaaa aatagaagaa caaattccga caagcgcatt
    14881 gcataaaagt gacaatgctt atacaagaga attaatagaa aaacaactat cattctaagg
    14941 agtgagataa tgaaaggtgc aatggcttgg ccctttttga gattatatat attaacattg
    15001 atgttcttta gtgccaatgc aatcttaaac gtatttatac cgttacgagg gcatgattta
    15061 ggcgcaacga atacggttat cggtatcgtt atgggggcat acatgttaac agcaatggtg
    15121 tttcgaccat gggcaggaca aattattgct cgtgtcggtc ccattaaagt attaagaatt
    15181 attttgatca tcaatgccat agctttaatt atttatggtt ttactggatt agaaggttat
    15241 ttcgtagcac gtgttatgca aggtgtgtgt acggcattct tttctatgtc tttacagtta
    15301 ggtattattg atgcattacc agaggaacat cgttctgaag gtgtatcatt gtactcgcta
    15361 ttttcaacga ttccaaactt aattggacca ttagttgccg taggtatttg gaatgcaaat
    15421 aatatttcac tatttgcaat tgtcattatc tttatcgcat taacaacaac attctttggc
    15481 tatcgcgtga cctttgctga acaggaaccc gatacgtcag ataagattga aaaaatgccg
    15541 tttaacgctg taactgtttt tgcgcaattt ttcaaaaata aagagttgtt gaacagtggt
    15601 attatcatga ttgttgcatc gattgtattt ggtgcagtta gtacatttgt accgttatac
    15661 acagtgagtt taggattcgc gaatgcggga atctttttga caatacaggc catcgcagtt
    15721 gttgtggcaa gattttactt aaggaaatac attccgtcag atggtatgtg gcatcctaaa
    15781 tatatggtat ctgtactatc attattagtg atcgcgtcat ttgtagtggc atttggtccg
    15841 caagtaggtg caattatttt ctatggtagt gcaatattaa taggaatgac acaagcaatg
    15901 gtgtacccaa cattaacatc atacttaagc ttcgtcttac caaaagtagg tcgtaatatg
    15961 ttgttaggtt tatttattgc ctgtgcagac ttaggtatat cgttaggtgg cgcattgatg
    16021 ggacctattt ccgatttagt aggatttaaa tggatgtatc taatttgtgg tatgttagtc
    16081 attgtaataa tgattatgag tttattgaaa aaaccaacac cacgcccagc gagtagtctt
    16141 taatgaaaaa gtttaaagca tattaagtta atgaatattt aaattttaaa aggtatattg
    16201 agcatggcga ttcatgggag tgggacagaa atgatatttt cgcaaaattt atttcgtcgt
    16261 cccaccccgg caaggttgac tagaattgaa aaaagcttgt tacaagcgca ttttcgttca
    16321 gtcaactact gccaatataa ctttgtagag cattgaacat tgatttatgt ctcaggctct
    16381 ctatatggct cgtttttaga acgacagtat atctaaataa agcacgctta gaagtgagtt
    16441 gcgatactga cttttaagtg tgctttttga tgtgtatgga taatggataa tactctgaaa
    16501 tattttacat gaaaatatgc aaaacgagta taactgctaa ttgataaaaa tagctcacca
    16561 taaaattacg gtatgatttt aaatataagt aagtcgcact acttgctagt atcaatgctg
    16621 gaatgaattc ccaccatgta ttaatgtatg gataatagta cagtgtttca agaataacgg
    16681 ataatactat tgtaatcttt aaagggatta atctgcttaa ttcttgaatt aaaatatgac
    16741 ggaaaataag ttgacaaatc aaagtattta atataatggt taacgaaaat atagctatta
    16801 aactgatgga accataccct ttaatgagcg ggtaaatgtc aaagacagta aaggcatcta
    16861 catttagtgc gaaaatattg aaatgattta aaagtaaaaa gagtacgaca cttagtgtaa
    16921 atgatataag aatatgccat ttatatttag cactagcaac gatttgcgaa cgtatcattg
    16981 gaataaacgc atcttcatgc atccgacgaa aaatagctag tgaaataata actgcgagta
    17041 aatagctaat gttcattgaa ataggaaaag agaaacccca tggggcttgt tgagtgaata
    17101 cagctactaa cccaaaagtt aaaaagacga taatgattgg caagatgtta accaaaaata
    17161 tataaaggaa aataaatcca atatcacgtt tgaaaaaacg agattgttca gtagcgtatt
    17221 cttcttctat gtaatgttta tttgtatttg acatagtata cctcttaaat agttgtatta
    17281 tatagatact ttagcacata ttactttgta ttgtatgttt tatacattaa aatttaaaat
    17341 gaaaaacata tcataaaatt gttttataaa atgaaacgct tccattgtgt tttattttgt
    17401 aaggtgtatc ataaatattg aattgaaatt ttttggggga gggattgtaa tgacgtttct
    17461 tacagtcatg caatttatag ttaacattat cgttgtaggc ttcctgctta cggttattgt
    17521 tatcgggctt atttggttaa ttaaagataa aagacaatca caacatagtg tattaaggaa
    17581 ttatccttta ctagcacgta ttagatatat ttcagaaaaa atgggaccgg aattacgtca
    17641 gtatttattt tctggggata atgaagggaa acctttttca cgtaatgact ataaaaatat
    17701 cgttttggct ggaaaatata actctcgtat gaccagcttc ggtactacta aagattatca
    17761 agacggcttt tacatacaga acacaatgtt tccgatgcaa cgtaatgaga tttcagtaga
    17821 taatacaaca ttgttatcaa cattcattta taaaatcgcg aatgagcgtt tatttagtcg
    17881 tgaagaatat cgtgtaccga caaagattga tccgtattac ttaagtgatg accatgcaat
    17941 aaaattaggt gaacgtttaa aacatccatt tattttaaaa cgtatcgtag gacaatctgg
    18001 tatgagttat ggcgctttag gaaaaaatgc tattacagct ttatctaaag gtctagctaa
    18061 agcgggcact tggatgaata caggtgaagg tggcttatca gaatatcatt taaaaggtaa
    18121 tggggatatc attttccaaa ttggtcccgg tttatttggt gttcgtgata aagaaggtaa
    18181 ttttagtgaa ggtttattta aagaggttgc acagttatct aacgtacgcg catttgagct
    18241 gaagttggca caaggtgcta agactcgtgg tggtcatatg gaagctgaaa aggtaaacga
    18301 agaaattgct aaaatccgaa atgttgaacc ttataaaaca atcaattcac ctaaccgtta
    18361 cgaatttatt cataatgctg aagatttgat tcgtttcgtc gatcagttgc agcaattagg
    18421 tcaaaaacca gtaggattca aaattgtagt aagcaaagtt tcagaaattg aaacacttgt
    18481 acgtacgatg gtggaactag ataagtatcc aagctttatt acgattgatg gtggtgaagg
    18541 tggtactggt gcaacattcc aagaattaca agatggtgtt ggcttaccgc tatttacagc
    18601 tctacctatt gtgtctggca tgttagaaaa atatggtatt cgagataaag tgaaattggc
    18661 ggcatctggt aagttagtga caccagataa aattgcgatt gcactaggtt taggtgcaga
    18721 ttttgtaaat atcgcacgtg ggatgatgat tagtgtcggt tgtataatga gtcaacaatg
    18781 tcacatgaat acgtgtccag ttggtgttgc aacaacagat gctaaaaaag aaaaagcatt
    18841 gattgttgga gaaaagcaat atcgtgtcac aaactatgta acaagtttgc atgaaggctt
    18901 attcaatatt gcagcagctg ttggcgtatc cagtcctaca gaaattactg ctgatcatat
    18961 tgtatatcga aaagtcgatg gtgagttaca aacgatacat gattataaat taaaattgat
    19021 tagttgattt atctatttcg agaaaatgaa agcagcggat tttagtgtta ctgcaaataa
    19081 ttttatatta gtagtggatg ctggtcacac aagaatttca aatattaaag ccctcagaat
    19141 atgagttaag gtttgtaacc ttagtcttat ctgagggcat ttttatgtta taaactattt
    19201 gtcgtccatt ttatcttttt cttttaagcc tctgtgcttt aattgctttt caagtttttc
    19261 aaaactaata tctttatttt ctttagttga aacaccaaga cgtttattta attttttcat
    19321 gtcaacttct gtgtaatcta tgtctaagtg ttcaattgct tttttatctt tatagtctac
    19381 tttgtatttt acgcctttaa ggtctttgaa aatactttca gatttggcga ataacttttt
    19441 ggcttcgtct ttatccatac ctagatcgtc atatttaatt gtgttgattg tagactgttt
    19501 taaaacttta tcatctttat atgtgataga agttagtaca tgtttaccac taacatcacc
    19561 ttcatatgtt ttggtttgtt ctttaccaca agctgataat gcaatgatac aaactaatgc
    19621 tactacaatt aatgaacata attttttcaa agtcagtcgc cttctttcga tatttgtatt
    19681 ataaagaaat tataacattt actaaaaaat gatgttattc aaaaatttaa attttgtcac
    19741 tttttttgaa gatatgagtt tttttaagcg gattcctcac aaaattttaa aaatatttaa
    19801 gcctgaaaat gataaagcgg tagggaacgt agtgtgttcc actgctgatt tctttttgag
    19861 cattatagac ggtatgttgg aagaaaagaa caaaagcttg tgtacgatag catataggta
    19921 ttaagaatac gatgaactcg ttggtaatat tgacgtactc aagtgcgtac gctataatta
    19981 gcgtaacaga aaacaaagga gggtttatat gattatcact agccctacag aagcgagaaa
    20041 agatttttat caattactaa aaaatgttaa taataatcac gaaccaattt atattagtgg
    20101 caataatgcc gaaaataatg ctgtgattat aggtttagaa gattggaaaa gtatacaaga
    20161 gacaatatat cttgaatcta ctggtacaat ggacaaagta agagaaagag aaaaagataa
    20221 tagtggtaca acaaatatag atgatattga ttgggataat ctttaatgag caattacacg
    20281 gttaagatta aaaattcagc gaaatcagat ttaaagaaaa taaaacattc ttatttaaag
    20341 aagtcatttt tagaaattgt tgagacttta aaaaatgatc cgtataaaat aacacaatct
    20401 tttgaaaaat tagagcctaa atatttagag cgatattcaa gaagaattaa ccatcagcac
    20461 agggtcgtct ataccgtaga tgatcgaaat aaagaagtat taatactatc ggcatggtca
    20521 cattatgatt aatgaatatt caatatctga ataactttaa tgataagtta attaaagaaa
    20581 ctagtattta agtgtaggga aaatagcgac gttaatgcgt tgttatctct acacttttta
    20641 attttataat agcgcaagac taaacagatt gaaattagta acaataaaag aataacgtat
    20701 tataataagg gattttaaat tgtgactttt tcggaatatt aaattttaga aatatgagtt
    20761 tttttaagcg gattcctcac aaaattttaa aaatatttaa gcctgaaaat gataaagcgg
    20821 tagggaacgt ttttctgaaa gttagtgata caatagttta aagttgaaat acaggaggat
    20881 gaataacatg aatcagtcag tcaaattact taaacattta acagatgtaa acggcattgc
    20941 tggttatgaa atgcaagtta aagaagcaat gcgtaactat atagagcctg tcagtgatca
    21001 aattattgaa gataacttgg gtggcatttt tggaaagaaa aatgctgaga atggtcaata
    21061 ctcaattatg atttctggtc atatggatga agttggtttt atggtaacaa agattgataa
    21121 acatggtttt atttcattta cgccagttgg tggatggtgg aatcaagtca tgctatctca
    21181 aaaagtaacg attacaacag attcgggcaa agaaattaga ggtatcatcg gttctaaacc
    21241 gccacatgtc ttaacgcctg aagaacgtaa aaagccaatg gaaatcaaaa atatgtttat
    21301 agatattggt gttagtagca aggaagaagc tgaagaagct ggcgttgaag taggcaatat
    21361 ggttacgcca tatagtgaat ttgaagtgct tgcaaatgat aaatatttaa ctgcgaaagc
    21421 atttgataat cgctatggct gtgcattagc tattgaggta ttaaaacgtt taaaagatga
    21481 aaatattggc attaacttat acagtggtgc cacagtgcaa gaagaagttg gtttgcgtgg
    21541 tgcgaaagtg gcagcgaata cgattaaacc agacttggcg atagctgtcg atgtaggtat
    21601 tgcttatgat accccaggta tgtcaggtca aacgagcgat agtaaactag gcggtggtcc
    21661 agttgtcatt atgatggatg ctacaagtat tgctcaccaa ggtttgcgta agcatattaa
    21721 agatgtagct aaggaacata acatcgaagt acaatgggat acgacaccag gtggaggtac
    21781 agatgcggga agtattcatg tcgcaaatga aggtattccc acgatgacaa tcggtgttac
    21841 gctgcgatac atgcattcta atgtttcagt gctcaatgta gatgattatg aaaattctat
    21901 ccgtcttgtt actgaaattg tccgttcatt aaatgatgaa agttataaaa atatcatgtg
    21961 gtaacccaat ccacaaataa taaagaatcc ttttaatatg gtaggttgtt aaacaattgt
    22021 ctaattttaa ttcttagtca ttagacagta tccatgttaa taggattttt tgtttttaat
    22081 ttaaatgctg aaaatcaatt atgcctaaat tttgatatta caagaaaatg attttttctt
    22141 aaatataatt gcactaaaaa ccaaaaaaac gggaataata tacctgatat attacatgag
    22201 gagcggtgca aatgttgtta gaaattaaag atttagtgta taaagcgagc gatagaatca
    22261 tactagatca tatcagtcta aaagtagata aaggcgagag tattgccatt ataggtccat
    22321 caggtagtgg taaaagtaca tttcaaaagc aaatatgtaa tttgattagt ccaactagtg
    22381 gagaacttta ttttaaaggt aaaccctata atgattatga cccggaagaa ttgcgtcaac
    22441 gaatcagtta tttgatgcag caaagtgact tgtttggtga aacgattgaa gataacatga
    22501 tattcccatc acttgcacgt aatgataaat ttgatagaaa acgtgcaaag caattaatta
    22561 aagatgtcgg tttgggacat tatcaattaa gttcggaagt ggaaaatatg tcgggtggtg
    22621 agcggcaaag aattgctata gcgcgccaac tgatgtatac accggatatt cttttattag
    22681 atgaatcgac cagtgcatta gacgttcata ataaagaaaa gatagaaaat atcattttta
    22741 agttagtgga acaggatgtt gctatcatgt ggattaccca tagtgatgat caaagtatga
    22801 gacattttca aaagcgcata acgattgttg atggcaaaat ttctaaggtt gaggagttga
    22861 atcaacatga gtaatacggc acttggattg acggcattgc ttttagtcat tccgattatc
    22921 atttcatata aagaaggttt acatattatt aaagatttaa ttgttgcgac actacgcgca
    22981 attgtgcaat taatcatttt gggatttttg ctgcattata tttttaaaat aaacgataaa
    23041 tggctgctta ttttatgtgt attggtcatt attattaatg catcatggaa tacaattagt
    23101 cgagcatcac cagtgatgca tcatgtgttt tggatatcat ttctagctat cttcattgga
    23161 acggcattac cgcttgcagg tactattgcg acaggggcca ttcaatttac agccaatgaa
    23221 gttataccta ttggcggcat gcttgctaat aatggcttga ttgcaattaa tttagcttac
    23281 cagaatttag atcgtgcatt cgtacaagat ggtactaata ttgaatctaa attatcactt
    23341 gcagctacac ctaaattggc ttctaaaggt gcaatacgtg aaagtattcg tttagctata
    23401 gtgccaacaa ttgattcggt taaaacatat gggcttgtgt cgattcctgg tatgatgaca
    23461 ggcttaatta ttggtggcgt accaccttta caagcgatta aatttcaatt gttagtcgtg
    23521 tttattcata caactgcgac cattatgtct gctttgattg cgacatattt aagctatggt
    23581 caatttttca atgcaagaca tcaattagta gcacgaaata ctgatgttaa gagtgaatca
    23641 tgatagattt tactgcatca gatttaggta ttagttttaa ttggaaatga agtgacgcgc
    23701 acatatagta tcgctattca ttaacacagc gaaaatattc ataaaggcac gcatactttg
    23761 tagtcagtta tctgttctga catataaagc gtgcgtgctt tttgggagtc attgttgaaa
    23821 ctgaagtaat tatacataat tattaaatga catacttgtg ttaatttttc aaatactgaa
    23881 aaacaatttc aataattttc caattaagca cagaaaatta aagcaaaata ttatataata
    23941 gaacggttat atataaaatt gtgttgcaca cattttttaa taaatcgtta ttctaaggga
    24001 aatgaatatc ggaaattttg tttgaaagga gttttaaaat gtcaatcatg cgactattta
    24061 cattcatttt aagtattttt atcgtaggaa tggttgaaat gatggttgca ggaattatga
    24121 acttgatgag tcaggactta catgtatcag aagctgtcgt tggtcaatta gtgacaatgt
    24181 acgctttaac atttgcgata tgtggaccta ttctggttaa attaacgaac cgtttttcat
    24241 caaggcctgt attattatgg acattactta tatttatcat tggtaatggc attattgctg
    24301 tagcgccaaa tttttcaata ttagtagttg gtagaattat ctcatctgca gcagcagcac
    24361 taattatcgt aaaagtatta gctattacag cgatgttatc agcacctaaa aatcgtggta
    24421 aaatgattgg acttgtctat acagggttta gtggggccaa tgtttttggt gtaccaattg
    24481 gaacggttat cggcgattta gtaggttggc gctatacatt tctattctta attattgtga
    24541 gtattattgt tggcttcttg atgatgatct atttaccgaa ggatcaggaa atacaacgag
    24601 gccctgtgaa tcatgagaca ccatctcatg aaaatcatgt tacttcgaaa atattaagac
    24661 ctgctgaagt agcaaaatat ttaattatta cgtttttagt attgattgca aactcagtga
    24721 cattcgtctt tataaatcca cttattttat ctaatggtca tgatatgtca ttcgtttcat
    24781 tagcacttct agtaaatgga atcgctggcg ttattggaac atcattgggt ggtatattct
    24841 ccgataaaat tacaagtaag cgttggttaa tgatttctgt ttctattttt atcgttatga
    24901 tgttacttat gaatttaatc ttacctggtt caggtctatt gttagcagga ctatttattt
    24961 ggaatatcat gcaatggagt actaatccag cagtgcaaag cggtgtgatt caacatgttg
    25021 aaggcgacac aagccaagta atgagttgga acatgtctag tttaaacgct ggtattggtg
    25081 ttggcggcat tattggaggc ttggtcatga cacatgtttc tgttcaagct atcacgtata
    25141 cgagtgccat cattggcgca ttaggattaa tcgttgtttt cacattgaaa aataatcatt
    25201 atgctaaaac atttaaatca tcataattct catatgaaaa gcacgcctgc tatcaaattc
    25261 aggtgtgctt ttttagatgc gataacgtta ttgatatgtg cgataatagc gacgttcatt
    25321 atgatacatc ggccaaggca ttttaccgct tttagcaaaa ttagctaaat cattttgcat
    25381 ttgtcgactt aaaaatttaa ggtgagcagt tgttggatat tgatgtgctg ctaatatttg
    25441 taagtttccg aaccaaaaca ccatatctag aatatggtaa gcactgcgat agtgtgcgct
    25501 agaagtatca tgccatgcaa attcagcaag ccatagttgt gcgtttgaat cttgtatatt
    25561 gagttgttgt aaaaattgct tcatcggctg tttgaaataa caatgtgtaa tcgctaaaga
    25621 ttgttgcttc gccgtctgaa catcttcgta ttttaaagga atatcattta attccataat
    25681 gtcgataaag cgttgcggcg ataatttttt ctgttcacta gtaatataaa tatcgccttc
    25741 atcttttgta taacatgcaa aaattggttt cgttgttgga taattatttt gtatataatc
    25801 tgttttaata ggcgcatata ttaaatcaag ccctttagaa ggtcctcgag attgttttag
    25861 cttcgccatc agcataagaa tatcatctgt cgataatgat gtaacatcat ctgtatctaa
    25921 ataatcgagc atcaattttt ggaaatattg tgctttattg cgtgcactct taagggtgtc
    25981 taatcgtagt gcgccactta gtagaacgac tttatggaag tatggctcaa tgtcaggtat
    26041 tttaagtaaa gtcaaaatgc tcatactgcc tgcagactga cccattaaag taatgttatt
    26101 agcgtcgcca ccgaaggatt caataaattg atgcacccat tttatgacat tgatttgatc
    26161 tgaaaggcca ttattggaat gaaaaccttt attaaaatat gaccagtcta aatatcctaa
    26221 tgcgcctaaa cgataattgc atgtaataac gataatgtcg ttattttgta ctaaatgtgc
    26281 cggttgatag agttcggctg taccatgacc attttcaaaa ctaccaccat aaaaataaat
    26341 gatgacaggt ttcttcgtct gatcattatg ttgtttccaa atatttagat ataaacagtc
    26401 ttcatgttca gtaaaagttg tagattgtga ggagaaaaaa tcttctaatt tgttgtctgg
    26461 ttgcggtggg atgggttgta tttcagttgc atcaattggc tctgaccatt gtgtttttaa
    26521 cgtagaatgt ttaaagcgat tgtcatgaac tggtggttct gcataaggaa tgcctaagaa
    26581 gatatctaaa ccatcttgtg taataccatg aatttgacca cctgtagtat taatcttcat
    26641 atttattaac ctcctttatc atccccaatt taatcatacc acggtctata gatgataggt
    26701 gacaagtata acaagacaat tttgtgactg aatcagacga gtttgatatc tgttttaact
    26761 caaaccattt tcagcattat atgatgttct tctacagtga catttcatta caatatcact
    26821 ctcgccatct tgttatgtta gttatttata accgattttc aaaataaaaa accaaccaag
    26881 atgttgaaat caatggttgg ttaatgtgtg tgttgaaatc atatatggca ctgtatttta
    26941 aaatatttaa tgagatgact ttgattcatt atttagtgac tcacgcatct tcgtattgat
    27001 tcgtgttgcg cgacggaaat agacgctttc aaatgaactc gttgtaccga tgtaatagaa
    27061 tataagcgcc acaacaataa taacgataaa gtcaaatgga tagtgaatca tatttaagcc
    27121 tttaaattct ttgcttccta taaatgacag tattgatagc acgattaaat atacaataat
    27181 ccataagcta ccaccaattt gtttctttgt attacgccaa ttcatacggt attcatagaa
    27241 gaagtagatt ggtaatccaa gtataatgat taaaataact tcagccgttg ttggccacat
    27301 tgcccaatat atagctaatg aagctaatac aaatgataat ggtgccatta cttttaaaat
    27361 ttttgctcta aatggacgag tcattgttgg tcccattttt cttaatgcaa tcactgtcgt
    27421 tgggccagtt aaataggcta ctaaagttgc agtagaaata actgctgcta gcgtacccca
    27481 atctctaaat aatgtaacca taatcatact aatgatggca ttaaagatga ttgctacacg
    27541 tgggatatga tatttttcat tcatcttccc aagaaattta gggatatgtc catttttctc
    27601 cattgctcgt aaaactcgac ctgtaacggc gacaaatgac acgccagtac cgaatggtga
    27661 tacaaaagct tcaatatata gtaaaattgc gagccaatta attcctaata agatagctaa
    27721 atcagcaaat ggtgaattga agttgatgcc attccatcca ctatgttgta acattgattg
    27781 aggcatagaa gtgataaacg tactttgtaa aatgatgtac aacactgcac tgattgacag
    27841 tgagatagcg atgcctcttg cgatattttt ttcaggattt ttaatttctg aacccatatt
    27901 aataattgtc tggaatgaat tgaatgaaaa aataatccct gatgctgttg ttgcagcaaa
    27961 aatcggtgca cttccgtaag gcataaatgt gcttgccgaa tggccgtaat ttgaagtgtc
    28021 gaatcctgat agcatcaaca tgatgatggt taacatgggt acgcctaatt taaatacaga
    28081 aattaaactc gtaaatgatg ttaaaagttt tacggaccaa tagtttaata atgaaaaaat
    28141 aacaatgatg agatatacag ctagcaatcc gtatgtgcta atagagccat tttccattaa
    28201 atatctcatt ggtttcgccc aatgccacgg ccaagaactc atatattgaa cagctgacac
    28261 agcttcgata ggtattattg tcaccaaaga cacccaattc gcccaagcag caataaagcc
    28321 taataatgag ccatgtgtat actgggcata gttactcatg ccacctgatt gaggaaacat
    28381 tgtgccgatt tcaatgtagt tataggcaat ggttccaatg actaggaatc caagaaccca
    28441 tgatatgatt gctgctggtc cagctattga tgatgcttcc cacgcaccaa atagccaacc
    28501 agatcctatt aaagagccta accctaataa gactaattgg gatagattga ttttgtcgtt
    28561 gtgcaattgc ttgtctttat tcataaatac tccttcgtta ttgccaaaaa tacaaaaaat
    28621 aatatgcgac aattatacgc ttattataat aatagtcaaa tgcatttttt gtaagaacgc
    28681 aaaagcattt gtaaaattag ttttttataa aattaggata tataatacaa tgtgtattga
    28741 aaaacaaaat ggaagtgatg actatgagtg agaaacaatc aaatgaacaa gttgaagcat
    28801 ttttaaataa agagagtcaa tggcaagaag aatataaata tttgagagcg ttaatcttta
    28861 atgaaacaga attagaggaa gcgtataaat ggatgcatcc ttgttacacg ttgaataata
    28921 aaaatgtagt acttatccat ggcttcaaaa attatgttgc actattattt cataaaggtg
    28981 ccattttgga ggataaatat catacactca ttcaacagac tgaaaaggtg caagcagctc
    29041 gtcagttacg atttgaaaat ttaacagaga ttcaagcacg tagcgaagaa attaaatatt
    29101 atctagctga agcaattaaa gctgaaaaag ctggtaaaaa agttgaaatg aagaaaacag
    29161 aggaatatgt tattcctaaa gagttagaag ctaagttcga agaaatgcca cagttagagt
    29221 cttcatttta taaattaacg ccaggcagac aacatcaata tatatatcat attggacaag
    29281 ctaaacgcag tgaaacaaga caaaagcgtg ttgaaaagta tattaaccaa atactagaag
    29341 gtaaagggat gcatgataag taattaatga gtaaagcata ccggttatac aacaacatac
    29401 aagatgacac gaaacaacca atggctcatg ctgttggttg tttttttagg tgtgcctgtc
    29461 atgggcaaca ctttgacgtt ggaattccgt tacaagcttg ggagtaggaa atgttagcaa
    29521 aaggcaaggg tgtctacaat gaatgatgaa aatattaaaa tataaggatg actttgtgag
    29581 tggctgatgg acgtttgtcc gcctgtaaca atggatgcgt gtgcattatt acaaaaattc
    29641 gagttttgca ataatatttc acatttccga cacttttttg ctataaaaca accatttgag
    29701 cgataataaa ttcgctttta aaaaatatga gttatctatt tagttgccaa agataaaata
    29761 ataatgttta ataacatcat atagagtatg ttagttttaa atgtcgaata tacgaatgtg
    29821 caaacaaagt aatcggtaga aattcaacat acatagcgcc gtttactgtt aagtattcac
    29881 attacagatg aaaaatataa aattctacat aatcaagacc atgatgtgta cttgtttaac
    29941 ttatgactct atttgtttaa caattgcgat gatggtcttt ttattttatg cgtatcattc
    30001 gtcatatttt ttatgaggaa ggagaaatga ttatgttaag tattaagcat ttaacgaaaa
    30061 tttattctgg taataaaaag gcagtagatg acatctcttt agatattcaa tctggggaat
    30121 ttatcgcatt tattggaacc agtggaagtg gtaaaacgac tgctttaaga atgataaacc
    30181 gtatgattga agcgacagaa ggacaaattg aaattgatgg taaagatgtt cggagtatga
    30241 atcctgtcga attgcgtcga agcattggtt atgttattca acaaattggg ttaatgcctc
    30301 atatgacgat taaagagaat attgtattgg tacccaaatt gttgaaatgg actaaagaag
    30361 aaaaggataa acgtgcaaag gaattaatta aacttgtgga tttaccggag tcatttttag
    30421 agcgttatcc agcagaacta tcaggtgggc aacaacaacg tatcggtgtc gtaagagcac
    30481 ttgcggccga acaagatatt attttaatgg atgaaccttt tggtgcattg gatcctatta
    30541 cgagagatac gttacaagat ttagttaaaa cgttacaacg aaaattaggc aagacgttta
    30601 tctttgtaac acatgatatg gatgaagcga ttaaattagc agacaaaatt tgtattatgt
    30661 cagaaggtaa ggtggtgcaa tttgatacac cagacaatat tttaagacat cccgcaaatg
    30721 attttgtacg tgattttata ggacaaaata gactgattca agaccgtccc aatgacaaga
    30781 ctgtagaagg tgtaatgatt aaaccaatca cgatacaggc agaagcaaca ctgaatgacg
    30841 ccgttcatat tatgagacaa aaacgtgttg atactatttt tgtcgttgat agtgacaacc
    30901 atttacttgg attcttagac attgaggata ttaatcaagg tatacgagga cacaaaactt
    30961 tgcgtgatat gatgcaacaa catatttata ccgttcaaat tgattcgaaa ttgcaagatt
    31021 ctgtacgcac gattttaaaa agaaatatca gaaatgttcc tgttgtagat gatcaacagc
    31081 gtttagtagg actgattacg cgtgccaatg ttgttgatat tgtatatgac acgatttggg
    31141 gcgatagtga ggatacagtg caagcagaac atgtggggga agacactgcg tcctcaaaag
    31201 tgcatgagca acacactact aatgtcaaag tacgtgacat aggagatgat aaatcatgat
    31261 tgagttccta catgaacatg gtggacagtt gatgtcgaaa acactggaac atttttatat
    31321 ttctatagtg gcattattac ttgccatcat tgttgcagta cctataggca ttttattatc
    31381 aaaaacaaag cgaactgcca atattatatt aactgtggca ggtgtcttac aaactattcc
    31441 aacactagct gtacttgcta ttatgatacc gatttttggt gttggtaaaa cgcctgcaat
    31501 tgtagcgcta tttatttatg tattattacc tattttaaat aacacggtac tcggtgttca
    31561 aaatattgat agcaacatta aagaagctgg aaaaagtatg ggaatgacac aatttcaatt
    31621 gatgaaggat gttgaattgc cgttagcatt accgcttatc attggtggca ttcgtttgtc
    31681 gtctgtgtat gtaattagtt gggctacact tgcaagttat gtaggtgcgg gtggattagg
    31741 tgatttcatt ttcaatggtt taaatttata tgatccactg atgattgtaa ctgcaacggt
    31801 actcgttact gcactagcat taggtgttga tgccttatta gctttagttg aaaaatgggt
    31861 agttcccaaa ggcttaaaag tatctggata attaggaggc taagataatg aagaaaatta
    31921 aatatatact tgtcgtgttt gtcttatcgc ttaccgtatt atctggatgt agtttgcccg
    31981 gactaggtag taagagcacg aaaaatgatg tcaaaattac agcattatca acaagcgaat
    32041 cgcaaattat ttcacatatg ttacggttgt taatagagca tgatacacat ggtaagataa
    32101 agccaacatt agtaaataat ttagggtcaa gtacgattca acataatgcc ttaattaatg
    32161 gggatgctaa tatatcaggt gttagatata atggcacaga tttaacggga gctttgaagg
    32221 aagcaccaat taaagatcct aagaaagcaa tgatagccac acaacaagga tttaaaaaga
    32281 aatttgatca aacgtttttt gattcgtatg gttttgcgaa tacgtatgca ttcatggtaa
    32341 cgaaggaaac tgctaaaaaa tatcatttag agacagtttc agatttagca aagcatagta
    32401 aagatttacg tttaggtatg gatagttcat ggatgaatcg taaaggcgat ggctatgaag
    32461 gatttaaaaa agagtatggt ttcgactttg gtacagtgag accaatgcaa ataggtctag
    32521 tctacgacgc attaaactca gagaagttag acgttgcatt aggttattct acagatggtc
    32581 gaattgccgc gtatgatttg aaagtactta aagatgataa acaatttttc ccaccttatg
    32641 ctgcgagtgc tgttgcaaca aatgaattat tacgccaaca cccagaactt aaaacgacga
    32701 ttaataagtt gacaggaaag atttcaactt cagagatgca gcgcttgaat tatgaagcgg
    32761 atggtaaagg taaagaacct gctgtcgtcg cagaagaatt tttgaaaaaa caccactatt
    32821 ttgataaaca gaaaggtggt cataagtaat ggaaggtaat ttattacagc aattattcaa
    32881 ttattatgtt acgaactttg gctatctatg ggatttattt ttcaaacact tattaatgtc
    32941 tgtctatggt gtgctgtttg cagctttaat tggtattcca ttgggaatct tgcttgcaag
    33001 atacacaaaa ctttctggat ttgtaattac aattgcaaat ataattcaaa cagttccagt
    33061 cattgcaatg ttagctattt taatgttagt catgggctta ggttcagaaa cagtagtttt
    33121 aacagtgttt ttatatgcgt tacttccaat tataaaaaac acttatactg gtatagctag
    33181 tgttgatgcg aatattaagg atgctggcaa aggtatggga atgacacgca atcaagtgct
    33241 acgaatgatt gaattaccgt tatctgtttc ggttattatc ggtggcattc gtattgcctt
    33301 ggttgttgcg ataggtgttg ttgccgttgg atcatttata ggagcaccta cgcttggtga
    33361 cattgtgatt cgtggtacaa atgcgacgga tggcacaacg tttattttag caggtgcgat
    33421 tccgattgct atcattgcaa tcgtcattga tgtactatta agatttttag aaaaacgatt
    33481 agacccaaca acacgacatc gtaaaaatca atctaatcat cggccgcaaa gtattaatat
    33541 gtaatagtag aagatgttta taatttagcg atttcgtttc atgatttata aaaaatgagg
    33601 ctactcaagg agctcaaata atctttgagt agccttttta taggttgtgt ttgtatgcgt
    33661 ttacactaaa atggcaatta ttatcatgaa agtttttgga taaaaagcgt taattattgt
    33721 aaaaatgcta aaaaatgaga tgttttattt ataattttct gcaaatttat gatattgttt
    33781 cttaatatat catattaaaa atttgttttt cttaaacata ggaggcttat ctaattcatg
    33841 gacacatcaa aacaatttag aggtgacaac cgattgcttt tgggtatcgt tttaggggtt
    33901 attacctttt ggctattcgc gcagtcactt gttaatcttg ttgtcccatt acaatcaaca
    33961 tatagtagtg acgttggaac gataaatatc gctgttagct tatctgcctt atttgctggt
    34021 ttgtttatcg taggtgctgg tgatgttgct gataaatttg gtcgcgtcaa aattacttat
    34081 gtaggattga tattaaatgt tgtaggttca ttactcatca tcattacacc tttgccagca
    34141 tttttaatta taggtagaat aattcaaggt ttgtctgcag catgtattat gccatcaaca
    34201 cttgctatta ttaacgaata ttatattggt acaagaagac aacgtgcctt aagctattgg
    34261 tctattggtt cttggggtgg tagtggtatt tgtacgttgt ttggtggctt aatggctaca
    34321 tatataggtt ggcgttcaat atttgttgtt tcaattctat taacattatt agcaatgtac
    34381 ttaatcaaac atgcacctga gactaaagca gaaccaatca aaggtatgaa agcagaagct
    34441 aaaaagtttg acgttattgg tttagtcatt ttagtagtga cgatgttaag tttaaatgta
    34501 atcatcacac agacgtctca ttttggttta gtttcaccgt taattctagg tttaattgtt
    34561 gtgtttatct gttcattagt tggttttgta tattacgaaa ataaaataaa gcatccactt
    34621 gttgattttt caatttttaa aaatagagga tacagtggtg caactatttc aaacttctta
    34681 ttaaatggtg tagcaggtgg tgcacttatc gtcattaaca cgtattatca acaacaatta
    34741 ggatttaatt cttcgcaaac gggttatatt tcattaacgt atttaatagc agtgttgtca
    34801 atgattcgtg taggtgaaaa gattttatct caacatggtc cgaagcgccc actattacta
    34861 ggaagtggct ttacagtgat tgggttaatc ttattgtcgt taacattttt accagaaatg
    34921 tggtatatcg tatctagtat agttggatat ttattgtttg gtactggttt aggattatat
    34981 gctacaccat caactgatac agcagttgct agtgcgccag atgataagtc gggtgttgct
    35041 tcaggtgtgt ataaaatggc gtcatcatta ggaaatgcat ttggagtagc agtatctggt
    35101 acggtttata ctgtgttagc agctaattta aatttgaact taggtggttt cacaggtatg
    35161 atgtttaata ccttgctagc aattgttgca tttttagtca ttttactatt agttcctaaa
    35221 aatcaaacga atttgtaaaa ctgaaatgaa agcaagttat tatgtacgga ttttaaagga
    35281 aattttgtga aagtaagttt atcatacaca cttaatgtta cgtattgacg tttaatgtta
    35341 ggtgtgttct tttatagacg ataaaagctg tgtgcatatt aagcgaatga ttttcaaatt
    35401 gacgctaata tgcgaaagta gtatttttaa aatgaacaac aacgatgaag aggggtttat
    35461 aggatgaaaa ttgcaattgc tggatcgggt gcattaggta gtggctttgg tgccaaacta
    35521 tttcaagcag gatatgatgt cacacttatt gacggatata catctcatgt tgaagcggtt
    35581 aagcaacatg gattaaatat aacgattaat ggagaggcat tcgagttaaa cattccgatg
    35641 tatcatttta atgatcaacc ggacgaaagc atttacgatg ttgtctttct atttccaaag
    35701 tctatgcaat taaaagaagt gatggaagat atgaagccac atattgataa tgaaacgatc
    35761 gtcgtatgta cgatgaatgg tctgaagcat gaagaagtca ttgcgcagta tgttgctcaa
    35821 tcacaaattg tcagaggtgt tacgacttgg acggcaggtc ttgaaagccc tggacacagt
    35881 catttacttg gtagtggacc agttgaaata ggtgaactag tggatgaagg taaagaaaat
    35941 gttataaaag ttgctgattt acttaacgaa gcggaattga atggtgtcat tagtaaagat
    36001 ttataccaat cgatttggaa aaagatttgt gttaatggta cggcaaatgc attaagcaca
    36061 gtgttggagt gtaatatggc atcgctgaat gaaagtagtt atgcgaagtg tttgatttat
    36121 aaattaacgc aagaaatagt gcatgtagcg acgattgata atgttcattt aaatgttgat
    36181 gaagtatttg aatatttagt tgatttaaat gaaaaagttg gtgcgcatta tccatccatg
    36241 tatcaagatt taattgttaa taatagaaaa actgaaattg attatattaa tgacgcagtt
    36301 gcaacattgg gtaaacaacg tcatattgaa gcgccagtca atcgctttat tactgattta
    36361 attcatacta aagaaagtca gcgacacgca caggattaat tggtgtttta ggtatgatta
    36421 caaggaaatg acaatcacgt gatattacgg tcattattaa gattgaaatg taataaataa
    36481 agaacagcag taaggtactt tcaaattgaa atgatcttgg tgctgttttt cttgattgat
    36541 tttcgtcata attcagattt gtcataggtc tacgacatac tattagtatt tactagacag
    36601 tttttacgac gacactttga aaaattttga ggcaaatcat ttggaagtct cacgtgaatt
    36661 ttgtaaactc atcaagcaag taattatatt aaaaagacaa atagagaaaa ggtgtttata
    36721 atgagtaaaa tttttgtaac tggtgcaacg ggccttattg gcattaaatt agttcaaaga
    36781 ctaaaagaag aggggcatga ggttgctggt tttactacat ctgagaatgg tcaacaaaag
    36841 ctagctgctg ttaatgtaaa agcatatatt ggtgatatat taaaagctga tactattgat
    36901 caagcgttag cagattttaa accagaaatc attatcaacc aaattacgga tttaaaaaat
    36961 gttgatatgg cagcaaatac gaaagtacgt attgaaggtt ctaaaaactt aattgatgcg
    37021 gcgaaaaagc atgacgttaa gaaagtaatt gcccaaagta ttgcctttat gtatgaacct
    37081 ggcgaaggat tagcaaatga ggaaacttca cttgatttta actcaactgg tgatagaaaa
    37141 gtaacggttg atggtgtggt tggtctagaa gaagaaacgg ctcgtatgga tgaatacgtt
    37201 gttttacgtt ttggttggtt atatggccca ggtacttggt acggaaaaga tggcatgatt
    37261 tataatcaat ttatggatgg tcaagtgaca ctttcagatg gcgtaacatc atttgtgcat
    37321 cttgatgatg cagttgaaac atctattcaa gctattcatt ttgaaaatgg tatctataat
    37381 gtagcagatg atgcacctgt taaaggttct gaatttgcag aatggtataa agaacaactt
    37441 ggtgttgaac caaatattga tattcaacct gcgcaaccat ttgaacgtgg cgtaagcaat
    37501 gagaagttta aagcgcaagg tggtactctg atttataaaa cttggaaaga cggcatgaat
    37561 ccaattaaat aataatttat ccgtttaata tacaaagaat aaagacttgg tcgaatcgtg
    37621 gatgatatat tatcaaacgc acggctcgaa caagtctttt ttattatgtc ttcgttatct
    37681 ttgtatgaag gaataacaga attacaatta atgtactgaa taatgcaatt aatgttgtga
    37741 ttagtgctaa tttaatttct attggtagcc aagtcagtac aaaagaccaa ttattgctac
    37801 cgagaatgag atatggtaat gcatataata tgagcgctat agcgatacat aaacataaag
    37861 ttagccaact caatacagca atccgttttg atctacgcaa aagaatttga ccatggcgca
    37921 tttgccaagc acgataagct gagaatataa atgctaataa aataagtgtt gtcatcaaaa
    37981 cggtaacaat attaaattgg tctttatatt gattgagtat agacgcaacc gtcgaatatc
    38041 gcttgtgatt tacaatttgt gtattaagat gctcaactaa tttgggtaca tattccgaat
    38101 ttagatttgc aagcacaaca attccataat tttgttttgg atttagtaaa ataaatgatg
    38161 aaaagttatc gagcgttcct gaatgaaaca ctaaatgttc atcattattg gtaaaccagc
    38221 ccgaagcata tgcattggca ttaggttcac caattgttga tgataaattt ttatgtgatt
    38281 gttgaactaa tgatttgtat ttatcaggtg gattaagttg gaattttatc caatgttcca
    38341 aatcttcagt tgatgtcatc atatatgctg atggtgtatc ccaaaggtta aattcaggtt
    38401 tagagacgac aggtgtcgaa ccttgtaatt cgtagccaat agcatcatgt tttgatttgt
    38461 aatttgtttg tttaaatgtc gtgtgtttca tttgaagtgg tttaagccaa tggtctgtta
    38521 tatattgcgt atatgattgc tttgtgacat tttggataat taaacctaat aaatcatagt
    38581 tcatatttga gtattcaaat tcttctccgg gcttatgatg taattcatca cccataattg
    38641 cacgggttac atcatttaaa cgattatttt tatttgttac ggcgtcttcg cttgtaatat
    38701 cactaggtat accacttgtt tgagccaaaa gttgcttaat cgtaatagtt tcattttgac
    38761 cattatagtt cattttaaaa tgaggcacat gtttggatac ggcatcattt aagtttaatc
    38821 gaccttcttg agctaatttt aaaattgcaa gacctgtgaa agctttcgta ttagaagcaa
    38881 tttcatactt tgttgtggga gaagctttga cttttttatc aacatcttga taaccataac
    38941 ctttatttaa aaaaactttg ccatttttta ctattaaaat tgatgctcca ggaatgtgtc
    39001 ccttttgtaa atcatgctcg ataattgtat ctatttgttg ttgcgaatca ttggttaacc
    39061 gtgtcttcgt attgctattt aatgttatat gtatagcaat tgaaatggcg actaaaatga
    39121 taatagaaat ggatagaaaa tacagttttt tagtagtcat aaacgttcct ttttatatgt
    39181 taaatttact taagtgccta atgagattta ttttattata ccagttctat tgaataaatt
    39241 aattaaagag gtatatttac cattattttt gacaccttat atgttaatag gtgtataatt
    39301 acataaaaaa tattaattaa ctctctatac tttttggaaa gataggcgta tagtgatatc
    39361 catcaaaaac tataaaaagg aataggagat tatgttcaat caatttaaaa gacttattat
    39421 agggcaacct aaaaaaaaca gagaactaaa agacgaaaag atatcgaagt ttaaagggtt
    39481 agcgattctt tcgtcggatg cattatcttc agtggcttac ggtcccgaac aaatactgat
    39541 tacattatca gtagtgggtg cagttgcaac ttggtatact ttaccgattg caggtgctgt
    39601 tctgatctta ttagctgctt taattatgtc ttataggcaa attatttatg catatcctaa
    39661 aggtggcggc gcgtatatgg tgtccaagac caatttagga gaaaaatggg gattgcttgc
    39721 aggaggctca ttattagtcg attacatact gactgtcgct gttagtatat catctggtgc
    39781 tgatgcattt gttgctgcat tcccaagttt atatgggcat aaagtactga ttgcatgttt
    39841 acttgtactt tttattttaa tattaaattt acgtggttta actgaatctg ccaccgtatt
    39901 atcctatcca gtatatcttt tcattatagg gttagtgata ttaatattta taggtacttt
    39961 ccgtgtggcg acaggagata ttcaaccaca tatgcatgca tcagtaggaa ctgcggttcc
    40021 tggagtaaca ttattcttgc tattaaaagc attttcatca ggggcttcat cattaacagg
    40081 tgttgaagct atttcaaatg cggtaaccaa ttttagagaa ccaagtgcta ataatgctgt
    40141 aaaaacgtta attgctatgg gttcaatttt agcgttttta ttagttggta tcgttggttt
    40201 agcatatgtt tatggaatta tgccacaaac ggaaacgaca gttttatcac aattagctat
    40261 gcaaatattt ggtgataatg ctgccttcta ttttgtacag gcaacgaccg tgatgatttt
    40321 ggtattagca gccaacactg gatttacagc ttttccgatg ttagcggctt caatgtctaa
    40381 agataaatat atgcctagaa tgtttactgt tagaggtgat cgcttaggct actctaactc
    40441 gattattatt ttaggtgtat tagccattat tttaattatt gtatttgatg gtatgactga
    40501 agatttaatt ccattgtatg cggttggtgt attcattcca tttacactag cacaattcgg
    40561 tatggttatt aaatggattc atgaacgtcc taagaattgg ttaagtaagt tgtctgtgaa
    40621 cttactaggt ggtatcgtga catttattgt ttttatgatt ttactcataa ctaaatttag
    40681 ccaagtatgg ccgatactta ttttcttgcc gttcgtggtg attttcttct tgaaaattaa
    40741 caaacattat cgagatatcg cagaacaatt acgttctgac attgatgttc ttaatgtgga
    40801 cgtggtagat cgtaatttag caattgtgcc gataacaagt attacaacag cagttgataa
    40861 gtctatttat tacgcacaaa tgcttgcgaa taatgatgtc atagcagtac atgtatcatt
    40921 tggagatgaa gatgaaaaag cattccaaga gaaatggaaa cgtcatttcc cagatgtaag
    40981 attagttatt ttacattcgg aatatcgcag tattatacga ccaatttcac gttttatcga
    41041 taagattaat cgtaaagcaa acgatcaaaa ctatatgatt acagtagtga taccagaatt
    41101 tattactaag aaacgttggc ataatttatt acacaatcaa actagtttgc gtatgaaatt
    41161 gtatttaatt tatcaaaaga atgttaacgt atgtacaatt ccatttaaac ttaaaaaata
    41221 gattctataa tcaaatcgga ttgatgaaag gttatttact tttcatcaat ccgatttttt
    41281 gatgttgaat ataaaaaccc cgtacatatg agatgataat gactttgtct catatgtacg
    41341 ggggtattgt tatgtcttgc ttaatgtttg gactgtaaag acaagacaat ttcgttgtaa
    41401 ttgatatttt ctcgaatttg agctgcaacg atattgttga ttttattttt gcgaattaat
    41461 tcatctaaaa gatgacgttg taagtacaat gcttccaatt tgtggttatg gaacatagct
    41521 tgcggtatga tttgattgct gttttcttga cgttcttttc gacgttgtgc acgccacttt
    41581 aatgagcgac tcatatcata gtaatgatta atgactaagc taacctcaag tacatttgta
    41641 ctattttgtt cttcttttaa gcgtaaaata atattgtgat tcacaatacg cataattttt
    41701 tgaacatctt tgaatgaatt ttcaaattct tgcttcttag attctttatt taaggctttg
    41761 tagtgcttca tcgctttaag tctaagccat aacatcttga agaagtttgc aaatttttca
    41821 acaaatgatg cagtacggtg tgtctctgtt aattcgacaa tattgcggta attattaata
    41881 tcctgatatg ttgccttacc tttatcaatt aaacgctcaa gtgtagatgt ttcgattact
    41941 tttgcaatat cttctaaacg tttgagttct tttgtatttt gattatcagg ttctgaattt
    42001 aataaaaata acaattctcc atagtattgg tttaatactg ggcgataatt tgtatcgttt
    42061 ttgtcttttt tactttcgtt tttaaaatgc tggatcactt tttgaacaat gaaaattttg
    42121 gcagattgat aagtcatacc tttaaaagta gtatcttcgg cagatggtgt aattaaaggt
    42181 aaaacaattt gcgctaagat taaactaatt aataccatga aagatgcaat aaacaataaa
    42241 tcattacggt attcgaatgc ttgtcctttt gtaatgataa atggtaatgt aagtgccatt
    42301 gaaagtgaaa ttgtaccgtg aataccacac atggtcataa taaatgcgta acgcacacga
    42361 gaaggtggtg tttcatgtga atcatgttcc tcgtctagat aagattgtat atttttcggg
    42421 aaataaaaat ctttgtacca gaaataaacc caaacaaatc gacatgcata aattgcaata
    42481 gcgattaata tagtgatcac tattaaaaat gaaaagttgt caggctctgt ttggaaaata
    42541 tctatgacta cagtaggaat cataaaacct aatacaacaa atacaaaacc atttaaggca
    42601 taactaaatg tgttccaaat ttgatggtaa ttcatttgta gttccgtttg tgctctaatt
    42661 aaacggtcgc gttctaaacc atggattaga cctgcgatta caactgcaat gatacctgaa
    42721 gcatgaactt cttctgctaa aaagtatacg acaaaaggag ttaataattg aataaaagtt
    42781 aaggtattgt tatcttttaa acctttatta gcggttaagt ctatacgtat tctaacgaca
    42841 acgaatccaa taattgcacc aataagtaca cctagtattg ttgaaatgat aaattgttca
    42901 acagcttgga ataatgaaaa ggtaccagtt actaatgcag taacagcaat tttaaatgaa
    42961 atgatacctg ctgcatcatt gagtaaagat tcaccttcta aaatcgtcat agaaccttta
    43021 ggtaataatt ttccgcgtgt aatagcagat actgctactg catctgtagg acataaaatt
    43081 gctgctattg caaaagcggc tggcattggt aaggcaggcc aaatccaatg tataaaatag
    43141 ccaacaccga ctacagttgc aaacactagt gccattgaca ttaatagtat aggtttacga
    43201 tattctaata attttgttcg agagacgtgg gtaccttcca caaaaagtag tggcgcgata
    43261 acggcaaaca taaatacttc agaattgaat tggaaatcaa cttgtattgg aataatgaaa
    43321 ataacgacac ctaatgcaat ttgaataaag gcagtaggaa tttgcgggaa tcgattattg
    43381 ataaccgaac taataatcac agcaaaaata aaaattaaaa atgcttctaa tagtgccata
    43441 caatactcct caaaatttta atagttaata ttttatcact tttaaggcat aatgacatag
    43501 atatattgat aaaatgaagt tattttcaaa aaaactctag tatcggttga actgatacta
    43561 gagcgagatg tttaaattat tgattgtcat atctgaaatg accgctgtca ttttgtcgtt
    43621 gttcatatgc gagtttttca gcattcgttt tgtatttttt ataaaagaaa aataaaaata
    43681 taaaccagaa cggcgaaata taaatagctg ctcttgtttc gtcactaaag aataataaaa
    43741 tgaatacaaa gaagaagaac gctagaataa tgtaagcaat aggcttgcca ccaatcaact
    43801 taaatttact gtttttatgt gcctcaggat gctttttcaa atacattaga taagcgacaa
    43861 taatcattgc ccaaacaact aaaaacaaca cagttgataa cgttgtaacg tatatgaata
    43921 gttgaattgc attagggaaa atgtagttta acaaggctgc tataagtaat gcaattgatg
    43981 aaactaacat tgataaataa ggcacgccat gactattcgt tttatttaaa actttaggac
    44041 ctaacccttg ttgtgacagt ccgaataaga tacgactatt cgaatagata ccactatttg
    44101 tagcagaggc cgcggcagtt agcacgacaa agttaacgac acctgctgca aatggcacac
    44161 cgattaatgt aaagagttta acgaaagggc tactatttgg atcgatatca ttccaaggta
    44221 tgactgacat aattactaat agaccaccga tatagaataa taaaatacgg ataggtacat
    44281 tattaattgc tttcggtaag gttttttcag gatctttcgt ttcaccagca gttacaccta
    44341 taagttcaat accaatgaat gaatatacag caatttggaa tgacattaag aaaccaaatg
    44401 ttcctccagg gaacatgcca ccgtgactga ttaagtttgt gaatgatgca tgtccataat
    44461 gtgttttaaa tgagaaaaag attaatacaa gaccaacgat aaccatcgca ataattgtga
    44521 caactttaat aatagagaac caaaactcca gttcaccaaa taatctggca cctaataagt
    44581 tgaagctaat caagattaaa acaataaata gcacggtaat ccaatttggg acttgtggat
    44641 accaaaatgc aaagtattgt cccatcgcag ttaggtctga catacttgat acaatccaac
    44701 ataaccagta agtccagcca atgacaaagc caccaaaagg gcctaaatat tcatttgcaa
    44761 tatcaacaaa tgaattaaat cttgtattgc tcaacaacaa ttcgcctaat gcgcgcataa
    44821 aagcgaatag tacaacccca ataatcatgt atgtgaataa cagtgatgga ccagttaaag
    44881 atattgtttg acctgatcct aggaataacc cagttccaat tgcgccccca attgctatca
    44941 attggatgtg tctgttattc aactctctat gcaattttct tgccataatt ttcctcctca
    45001 ggataaagca aataaaaaag ttgtgtgtaa gcgttttctt aattataaat actaaagaaa
    45061 tccatttaat aaagcaatta tttttttaaa tataagaaaa aacatttgat aggtaagggt
    45121 atgataacta aaacttatcg attttttgaa aagatataag tttttatgca aaaatataaa
    45181 at
//
LOCUS       DAMZLE010000008        41470 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMZLE010000008 DAMZLE010000000
VERSION     DAMZLE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.008"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(394..492)
                     /locus_tag="POB92_000431"
     CDS             complement(394..492)
                     /locus_tag="POB92_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577957.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(697..1293)
                     /gene="recR"
                     /locus_tag="POB92_000432"
     CDS             complement(697..1293)
                     /gene="recR"
                     /locus_tag="POB92_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI6577958.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1300..1617)
                     /locus_tag="POB92_000433"
     CDS             complement(1300..1617)
                     /locus_tag="POB92_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI6577959.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1707..3404)
                     /gene="dnaX"
                     /locus_tag="POB92_000434"
     CDS             complement(1707..3404)
                     /gene="dnaX"
                     /locus_tag="POB92_000434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI6577960.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSELKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLNQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDAQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3473..3997)
                     /locus_tag="POB92_000435"
     CDS             complement(3473..3997)
                     /locus_tag="POB92_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6577961.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQALAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4135..4236)
                     /locus_tag="POB92_000436"
     CDS             complement(4135..4236)
                     /locus_tag="POB92_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577962.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSEIDVQFE"
     gene            complement(4309..4578)
                     /gene="ffs"
                     /locus_tag="POB92_000437"
     ncRNA           complement(4309..4578)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="POB92_000437"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4640..5368)
                     /gene="treR"
                     /locus_tag="POB92_000438"
     CDS             complement(4640..5368)
                     /gene="treR"
                     /locus_tag="POB92_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDI6577963.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5393..7033)
                     /gene="treC"
                     /locus_tag="POB92_000439"
     CDS             complement(5393..7033)
                     /gene="treC"
                     /locus_tag="POB92_000439"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI6577964.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKFDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKKILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLTILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7097..8524)
                     /gene="treP"
                     /locus_tag="POB92_000440"
     CDS             complement(7097..8524)
                     /gene="treP"
                     /locus_tag="POB92_000440"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDI6577965.1"
                     /translation="MAVKREDVKAIVSAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(8994..9086)
                     /locus_tag="POB92_000441"
     tRNA            complement(8994..9086)
                     /locus_tag="POB92_000441"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9048..9050),aa:Ser,seq:tga)
     gene            complement(9355..10818)
                     /locus_tag="POB92_000442"
     CDS             complement(9355..10818)
                     /locus_tag="POB92_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDI6577966.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEEPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10836..15335)
                     /gene="gltB"
                     /locus_tag="POB92_000443"
     CDS             complement(10836..15335)
                     /gene="gltB"
                     /locus_tag="POB92_000443"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDI6577967.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEAVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHIAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGIQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKVNSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15517..16401
                     /locus_tag="POB92_000444"
     CDS             15517..16401
                     /locus_tag="POB92_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6577968.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16516..17634
                     /locus_tag="POB92_000445"
     CDS             16516..17634
                     /locus_tag="POB92_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI6577969.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17631..18413
                     /locus_tag="POB92_000446"
     CDS             17631..18413
                     /locus_tag="POB92_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI6577970.1"
                     /translation="MNAVFILALLLLFLMIIFGGRKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18582..19067)
                     /locus_tag="POB92_000447"
     CDS             complement(18582..19067)
                     /locus_tag="POB92_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6577971.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFIGRPVHIMALTI
                     K"
     gene            complement(19057..19452)
                     /locus_tag="POB92_000448"
     CDS             complement(19057..19452)
                     /locus_tag="POB92_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI6577972.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESRVQALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19601..19870
                     /locus_tag="POB92_000449"
     CDS             19601..19870
                     /locus_tag="POB92_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577973.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20055..21059)
                     /gene="aaa"
                     /locus_tag="POB92_000450"
     CDS             complement(20055..21059)
                     /gene="aaa"
                     /locus_tag="POB92_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDI6577974.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21391..22233)
                     /gene="gmpC"
                     /locus_tag="POB92_000451"
     CDS             complement(21391..22233)
                     /gene="gmpC"
                     /locus_tag="POB92_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDI6577975.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     AITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22270..22929)
                     /locus_tag="POB92_000452"
     CDS             complement(22270..22929)
                     /locus_tag="POB92_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6577976.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22933..23958)
                     /locus_tag="POB92_000453"
     CDS             complement(22933..23958)
                     /locus_tag="POB92_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDI6577977.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPEELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24255..25397)
                     /locus_tag="POB92_000454"
     CDS             complement(24255..25397)
                     /locus_tag="POB92_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDI6577978.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVITALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25390..26295)
                     /locus_tag="POB92_000455"
     CDS             complement(25390..26295)
                     /locus_tag="POB92_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDI6577979.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            complement(26512..27849)
                     /locus_tag="POB92_000456"
     CDS             complement(26512..27849)
                     /locus_tag="POB92_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDI6577980.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28014..28127)
                     /locus_tag="POB92_000457"
     CDS             complement(28014..28127)
                     /locus_tag="POB92_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577981.1"
                     /translation="MGIAPEVIMWRDVGDIDGVVCGEILAHVYSVEPGILM"
     gene            complement(28305..29039)
                     /locus_tag="POB92_000458"
     CDS             complement(28305..29039)
                     /locus_tag="POB92_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDI6577982.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSDILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29076..29750)
                     /locus_tag="POB92_000459"
     CDS             complement(29076..29750)
                     /locus_tag="POB92_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6577983.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29788..30204
                     /locus_tag="POB92_000460"
     CDS             29788..30204
                     /locus_tag="POB92_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577984.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30433..30795)
                     /locus_tag="POB92_000461"
     CDS             complement(30433..30795)
                     /locus_tag="POB92_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDI6577985.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30957..33662)
                     /locus_tag="POB92_000462"
     CDS             complement(30957..33662)
                     /locus_tag="POB92_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDI6577986.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMADSYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKITQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDYVVARLLANNDHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33675..35159)
                     /locus_tag="POB92_000463"
     CDS             complement(33675..35159)
                     /locus_tag="POB92_000463"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDI6577987.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHMSNSTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35680..35745
                     /locus_tag="POB92_000464"
     CDS             35680..35745
                     /locus_tag="POB92_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDI6577988.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35777..35842
                     /locus_tag="POB92_000465"
     CDS             35777..35842
                     /locus_tag="POB92_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDI6577989.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35894..35962
                     /locus_tag="POB92_000466"
     CDS             35894..35962
                     /locus_tag="POB92_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDI6577990.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36026..36088
                     /locus_tag="POB92_000467"
     CDS             36026..36088
                     /locus_tag="POB92_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDI6577991.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36745..37947)
                     /locus_tag="POB92_000468"
     CDS             complement(36745..37947)
                     /locus_tag="POB92_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6577992.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTINSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLETMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38079..38339
                     /locus_tag="POB92_000469"
     CDS             38079..38339
                     /locus_tag="POB92_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577993.1"
                     /translation="MSTLLYNGDKDISHERESRCINMLFVILVLYIIGITFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38623..38937)
                     /locus_tag="POB92_000470"
     CDS             complement(38623..38937)
                     /locus_tag="POB92_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577994.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMNAQKENIRSVVAPEHK
                     HKYKDIENGLKGEEKVLIEQMAHHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38956..39279)
                     /locus_tag="POB92_000471"
     CDS             complement(38956..39279)
                     /locus_tag="POB92_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577995.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39276..40601)
                     /locus_tag="POB92_000472"
     CDS             complement(39276..40601)
                     /locus_tag="POB92_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6577996.1"
                     /translation="MKDKYKIDPGPIKYNTEETTAISKISYEIENAKLYGIKSKTIGR
                     QIDQLKEAKKFPSNLEYVDSYTDSLNGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKL
                     KEGEFSRQNFTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKHSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(40591..41403)
                     /locus_tag="POB92_000473"
     CDS             complement(40591..41403)
                     /locus_tag="POB92_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6577997.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKADELSNE
                     GMVLYLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMKNNKIILLDKVEDPKLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDER"
BASE COUNT        12963 a         8532 c         5981 g        13994 t
ORIGIN      
        1 aactttttaa aattaatttt cgttagtaat tcaagttatt tcttaagttg aattgttttg
       61 tttgcctcaa caaataaata ctatacaggc tttcaaactg cttaacaaca gtaaaaatta
      121 cacctttgtc atctgtactg aacgccctta tcctaaaaat cgaacttaaa aaaatcccca
      181 cttataaata gtacgttttt tagaacaaga aacagatttt tttctaacca tatcaatcaa
      241 aaaaaccgca aaacagaacg ttattttttt accagaaatt aaaaataaat ttacaagttt
      301 taaataatct ctcaaaacac cgctatacag gctgatttaa taagctttag accaaagaag
      361 tacaacttac taagccagca tctatcattt caattacaaa agaatttcat ttgcatagtc
      421 attaaccgca tttatcaaat taatagcacc aacacaccat aaccatagat ctaattcgat
      481 aatccaattc acatcattac tattagaagt aacaaatcac aaccaacggc ttgcttccta
      541 aagattaccc atccctcagt aaaactaggt aaaccatacc accgccacac cgcaaataaa
      601 gtagtacatc acaccacacc ataaaaaaag ccacaatgat tacactgtga cttttcttac
      661 tatagtatta aaatcaaaaa tgtttaatag aagacattac atttctgttc tacctgcgat
      721 tgctttagat aatgttactt cgtcagcata ctctaaatcg ccacctaccg ataacccttg
      781 tgctaatctc gtcactttga tacctatagg cttaactaat ctagaaatat acatggctgt
      841 agattccccc tctaagttcg ggttcatagc taagattaat tcgctaactt catcgttttt
      901 caagcgttca atcaatgaag gaatattaat atcttctggt ccaatgccat ccataggcga
      961 aatagaccca tgtaaaacgt gatataaacc tttgtattct ctcatttttt ccatagctat
     1021 gacatctttg tcatcttcca caacacaaat aactgaacga tctctttgct tatcttcaca
     1081 aatataacat ggatcatttt cagtaatgtg accacatacg ctacaatatg ttaattctct
     1141 cttaacatct actaatgctt tggcaaactg aacaacatcg tcttctttca tatctaaggt
     1201 atgaaaagcc agacgttggg ctgtctttgg accaatgcct ggcaatttca taaagctatc
     1261 aataagtttt gatataggtt ctggataatg catctatgat cacattccag ggatgtttaa
     1321 gccttgagta tgtttaccta aacgttcttg agtaagctca tcagctttat tcatcgcttc
     1381 attagtagct gctaacacta agtcttgtag catttcaata tcatctgggt ctacagcttc
     1441 ttctttgatt tcaacgtcga caacttcttt atgaccagtt acagtaactg caaccatgcc
     1501 accgccagct gttcctacaa tacgctcttc tttaagtttt tcttgttctt gagccatttt
     1561 cttttgcatt ttttgcattt gtttcatcat ttgttgcatg tttccgccac cgcgcatatt
     1621 ccatttcctc cttgaaaatc aataaatatt tatcaataaa atgatgtttc tttcattata
     1681 catacgatta tatcgcttgt catgtatcac tcttcatcta tcacatgtac agtttcttca
     1741 ccgaaaagat cttttgcttt ttgagcaata tctgtttgtt gtgcttgttg ctttggcata
     1801 tcatcgcctt cgtttttacg attttgtaaa tactccgttc gaactctttg ccattgatct
     1861 gatggtacac caacaacttt aacgttttta ttaacgatat tacatacaac actttctata
     1921 ctactacgtt tctcgtcgtc tttattgacg atttcacaat ggatctcttc ctcaaatttc
     1981 acaagtacgt gatcttcact tgccgccaca ggttccgaat tttgcaataa actaacgagt
     2041 gattttttat cattgttttt ggcatgatca atcacttctt gccaatgatc tttcaacaat
     2101 ttgatatctg ccttattcgc tttatctagc acttttgcaa tttgttgcat tgaaaatgca
     2161 tttttagatt tttgtatgcc tctcgcaggc tttttcgaag atttttgaac aggagcgaca
     2221 ctcactcctt gtgcttttag tgtttttagt tcttgctcta actgttccat acgttgcaac
     2281 aatacatctg tgtttggcga tgaagcaatt tgtgctggtt cagctacatt cgcaatcact
     2341 tgtggttgac ccttaatctg ctcagctaat tttactaaca acacttcaaa atgaacgttt
     2401 tgattcacac taaaacgaat cgacactaat gtatcattaa taagatcaat catttgatat
     2461 aacatatcta attctaagtt catcagtgct cgatactcag tatctttctc agatgtttta
     2521 ttcataatcg tatctctgac aaaataaatc atatcattta ttaggcgatt cacttcttta
     2581 ccctctgtta taaactgatg gtatttttta aaagatgctt gtgcgtcacc ttgtacaata
     2641 tcatcaaaca agtgatccaa cgcttcatca tgtacgctac ctgtgacatt caacgcatct
     2701 tgcaatgtta acgtaccatc accaaatgca atagcctgat ccataatact taatgcatca
     2761 cgcatacccc cttcagacgc tttagcgata aatgccaagg cttcatcttc acattcaatt
     2821 tgttgtgcat ctgctacaaa ttttaaacgt tcaacaattt gatttaggct aattgcttta
     2881 aaatcaaaac gttgtgccct agaaatgatt gttggaggga ttttatgtgg ttctgtcgtt
     2941 gccaatataa aaatagcgtg tgctggaggt tcttctaacg tctttaaaag ggcattaaaa
     3001 gcacctgttg ttagcatgtg cacctcatct ataatataaa ctttatattt caattcactt
     3061 ggtgcatatt taactttgtc tctaatattt cttatttcat caacgccatt attactagca
     3121 gcatcaattt ctatcacatc tgaattagtc ccctgcgtaa tgcctttaca aatatgacat
     3181 tcattacaag gttctccatc agtgctattt aaacagttga ttgctttagc aaacactttg
     3241 gcaatactcg ttttccccgt gcctctcgga ccactaaaaa tataagcatg cgactgtttt
     3301 tctttagaaa tcgcattgcg caatgtcttc gtgacatgtt cttgtccgac gacatcctcg
     3361 aaactttggg gtctgtacat acgatataag gcttgataat tcaagttagc acctccataa
     3421 acaattacct ctcattatag catgataata cctttacttc ttaattgaac aattaataaa
     3481 aatgttctgg aaattttacg attccgtgtg gtgcatccgt caaattatcc cataaatatt
     3541 ttacaagtaa cggtgcatca ccagattcta atttaatgtc atgctcagca gcattttgat
     3601 aacccaactt ttcaaagtag tcaaaacaat ggtctacaac aaccgtacta tactcttgtg
     3661 ctttggcacg ctcttctact gcttgaacca agccacgacc taatttttgt ccacgtaatt
     3721 caggatgaac tgataaagag gcaatcgcca aaccataata cgtcttatca tcactattaa
     3781 tttctacttc aattaataaa acgtgtccaa cgacatcgtt attttcattt ttcgctatta
     3841 cttctaattc aaaattatag caaggagatt ttcttaaatg ttttactttc gcaagtgctt
     3901 gccaactcgt ttcaggatta tcatcaaaac tttcttcaat actatttaaa gatttatcat
     3961 aatctaactc tgttaaagta cttaaatata tttgcatatg tcctccgtag gcatttgatt
     4021 gtcaataatc ataacgtatc tcattaataa ttctattgta agatactccc cctcatttca
     4081 ccatcatttc tttatcatca aataataact tcttactttt cattgacaca aaaatcattc
     4141 aaactgcaca tcgatttcac ttgaatcaaa cttcacatat aaaaaagcta cttcccacaa
     4201 acatgttcca cgtataatac gctgaattgt cttcaagaaa gtagcttcta taaataaata
     4261 tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc gtgcacctaa
     4321 gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg ctaccctacg
     4381 gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt tcatgggttc
     4441 atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt cgcaaaaata
     4501 cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag ggaaccgcta
     4561 cctccccacc tagcacggca agatatatat tactatattt taatagttaa ttgcaagtat
     4621 aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcgtta aatttaaatt
     4681 catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct aaatacacaa
     4741 tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga tatgcttgtt
     4801 catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga tttaatacct
     4861 tttccaaata atcataaata gaatcactcg caacatcatt acctatgtct gaaacaatcg
     4921 actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga cgagttctaa
     4981 caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact tctggaactt
     5041 catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg cccatttctt
     5101 cttgcatttc tttaaaacta accagttcag aaaatggaaa ctctgtaacc tcctgataaa
     5161 tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc aacaaatcta
     5221 atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca tgttcagacg
     5281 gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac gcctcataaa
     5341 tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta ttttacgcgt
     5401 ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact gtgcctgttt
     5461 gaatcacaac acgaccttct ctagccaatc cttctggcaa atcaacagcc gatgctgaga
     5521 aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca aataaatgat
     5581 catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc gtatgtctta
     5641 attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt gcttgtctaa
     5701 cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct gtaaatccag
     5761 cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac ttctgtccta
     5821 aaatcgttaa cacatccgct tcagcatgcc cttcacttaa caactgatgg taggcattaa
     5881 tcgattcaac atcgcgatat tgtgctattg atgtaaaatg tgggtccgtc ataccaattt
     5941 cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata gctaacattt
     6001 tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac actacccgtg
     6061 gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa atacctcgtt
     6121 gccattgcat cagaattttc tttaacttat ggaaatcaag cttcgcattt gtccactttt
     6181 caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc aattcttggc
     6241 gttctggctg tgtatactta atacaatttt caatcgtcgt cgaagacatt tctcctacag
     6301 tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac tcatgcactc
     6361 taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat tcacctttag
     6421 aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc caatgattga
     6481 ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca gcttgactga
     6541 catcaaataa atgtaaataa tattcatctg tctcagaatc aaacttccat gcattaccac
     6601 cgaatttaga atgccaattt gttggcggcc cgtcttcaga tgatctgaaa aagtaataat
     6661 ctctataagg gttgtcttta gatttacggg cttctttaaa ccattcatgc tccgtcgacg
     6721 tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgagca actttgatta
     6781 acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa ttgctgatat
     6841 catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata taatcaacac
     6901 ccaataactt gatataatcc aatttttcta taattccatt gatatctcct ataccattcc
     6961 ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt ctccaatcta
     7021 tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa ataacgaacg
     7081 cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta ctaaaatgag
     7141 acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca atgagatata
     7201 ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca ccaacttttc
     7261 caagtacgtt attcatacca acgattgccc ccaatacaca agatgttgat atcgcagcga
     7321 taaatggata tttcagaggt aagttcacac caaacatggc tggttcagta acacctaaca
     7381 taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta cgacgtttat
     7441 agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc gcaacgattg
     7501 gccataagta cgtaccacct aagctgctac ccatcaattg gaaatctact gctaaaaaca
     7561 tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt gctccgccaa
     7621 gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta ccaatcaata
     7681 acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc gctacgggtc
     7741 caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt tcaattttag
     7801 ctagaacata agctgcaatt aaaactggca acacttgacc ttggtaattc aactgcttaa
     7861 tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca tactgagata
     7921 ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga ctaccaccaa
     7981 atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc gtactcgcaa
     8041 tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata agtgcttttg
     8101 gaccaaataa acctttcatt gtaagtaaat tattgatccc cattaacaaa ccagctgtca
     8161 caatcgcagg taatattggt ataaaaatat cccctaacaa tttgatcaaa cgttgtactg
     8221 gattcccttt ttgcgcagct gcttgtttcg cttcatcttt cgaagcttct tgagcacctg
     8281 tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg acaatttgat
     8341 attgatggtc tgctttgaac tgccccttga ccaacgcgtt attacttaat gcgtctttat
     8401 caactttgct ttcatcttta agcactaaac gtaatcgtgt tacacaatgc gttgcagctt
     8461 caagattttc ttttccccca atagcgctta cgatggcttt tacatcttct ctttttacag
     8521 ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga caagttatgt
     8581 aaaggctttc aacaaatatt ttttattttg tcacaattat ctggggtagc catttcagaa
     8641 aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg cgtgattaac
     8701 aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact cctaaattga
     8761 aaaatgcata tctataaata gcaaacttgc ccacacaagc catccatcat atttcacaaa
     8821 acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca atctcaatgc
     8881 cctatcattt ctatagctaa aaaaacgagc cattcaaaga caaataaaaa aggcaaatac
     8941 tcaaaatttg agcacttacc ttcattatat atagtattta atataaatat tgatggcgga
     9001 ggaagaggga ttcgaacccc cgcgagccgt taagcccctg tcggttttca agaccgatcc
     9061 cttcagccgg acttgggtat tcctcctaaa cacaatattt atcatatatg aacgttctgt
     9121 aaaagtcaat attgttttta agcattacat gattaccaca aatcattgtc ttccactaca
     9181 gttgacctat aaacgaatgt aaaaaatcac tacttataat taatcgattt gttgtcttac
     9241 aaacctttgt ttttaggtta caaagatggc atgtgacgtt gatagatact agattatttc
     9301 aaactcgatt cagcatgcca caacgtcaac gtaaccacca tttccataca aagattatac
     9361 acaaacttta ctcgctaaat actgatctac tgctttcgct acgcctctac cttctttaat
     9421 cgcccataca actaaacttt gaccacgtct agcatctcca gcagcaaata ccttttcatt
     9481 attagtttga tagtttgtat catccgccac gattcgattt ctatccgttt taatgttaaa
     9541 agcattcggt actgttggtt ctgtaccttc gaagccgatt gataataata caaggtcagc
     9601 aggccaaaat ctttcaggtt cttctttcat gaccatacca ttttcgcctt gctctaaaat
     9661 ttgagtatac aaaccacgta tgtgtccttt atcgtcaaca tcgtaacgca ttgtttgaac
     9721 accatatgca cgtggttcct taccaaactt agcttcgtac tcttggtgcg catagtccat
     9781 tttaaacacc ggcattgcta aaggccatga tgcattttct gtaaatgtaa ttgcttctgg
     9841 caatttcgta tatttattaa attgaacaat cgatttacaa ttttctctta atgctgtcgc
     9901 tacacagtct gcccctgtat caccagcacc aatgataatg acattcttat cttttgcagt
     9961 tattgttata tcatcaattt ctccatttaa caactgcgtt tgttcagtta aataatccat
    10021 agcgaaatgt ataccatcac ccatgcgtcc ttctaaaggt aaatctctac ctttttgtgc
    10081 accagtacat aatataatgg catcatactc agattctaac gttgctttat caatatcaac
    10141 gcctacttca acaccattaa tgaaagtaat gcccgcttct tccattaact taatacgacg
    10201 tcgaaccaca tctttatcaa gtttcatatt cggaatacca tacattaata aaccgcctga
    10261 ttctctagca cgttcataaa tagttacttg atatcctagt agattaagtt cttcagcagc
    10321 agctaatcct gctggaccgc ttccaacgat tgccactttt tcatctctac ggcgactcgg
    10381 aactttcggc gctacccaac cattttcaaa agcttcatca ataattgtgc gttcaatacc
    10441 tttaatcgca atcgattctc tattaatctt catcacacaa gcactttcgc atggtgcagg
    10501 acatacacgc cctgtaaagt caggaaagtt atttgtttcg cttaagcgtt cataagcagt
    10561 tttaaaatct tgatgataca ctaagtcgtt ccattcagga atgtagtttc caattggaca
    10621 acctattgtt tccctaccat actgttgtcc ggtttgacaa aacggcgttc cacaatccat
    10681 acatcgtgca ccttggatag aggcatcttc tttagtaaat cgttgttgat atgctttatg
    10741 atgcttcaaa cggtctacca gtgataattc acctaagtac tgtttgtcat acttcataaa
    10801 tcctttaaat tcacccatcg tatctccccc tttccttaat acacaacggc tggtttatgt
    10861 ttagcgtcga ttgttttact gtcatcgtaa aatgcagcta acatcgcttc atcttcattg
    10921 tcatgtaatg atttgtgcaa atgaattttt tgcatcatta attgataatc tttaggaata
    10981 actttaacga cgacatcttc aatgcgatca aaatgtttta acacatgaat cgctctcgta
    11041 ctattcgtgt gtgacacatg ttcttccagc atttgcttaa tgaatgcttt ttcttcttgg
    11101 tgtttaatct ttgtaaacga aagcgtatct agttgattat tttcaacaaa agcttctaca
    11161 tcagacggga taacgtaagc aataccacca ctcatacctt gaccgaagtt cttacctaca
    11221 tcacctaaat taatgacatg tccaccagtc atgtactcta atccatggtc accgatacct
    11281 tcaacgacaa catctacacc actatttcta atacagaatc tttctcctgc actaccgtta
    11341 ataaatgcct taccacctgt cgcaccatag aatgagacgt taccagcaat aatttcattt
    11401 tgtcgttctt caaaaggtgc tttgacaatg accgtaccac cagataatcc tttaccaaca
    11461 tagtcattcg catctccagt atgatgaatc attaagcctt tcggtgcata tgctgcaaga
    11521 ctttgaccag catgaccatt cgtataaaca ttaattgtat tttcaggaag tcctgcttct
    11581 ccatattgtt tcgaaatctc actacctgta ataactccta catcacgttg ttcattattt
    11641 actgtaaagc tacctgtata gcgacgccct tcagcaatat acggcttcgt tacttcatat
    11701 aaatttgtta aatcaaatcc atgctcaaga ttatgatttt gttgaatttc ttttgtgttt
    11761 ggcccatcga aaggacataa cagtttttca acatcaatac tagccgcttt gctattcact
    11821 tttaatgttg atgatcgttg taataaatca gttcttccaa ctaagtcttc tacacgtttc
    11881 aaacctaaag atgctaaaat ttctcttaat tcttgtgcaa taaaatgcat aaaattaaca
    11941 acatgatgtg ctttacctct atataaagca cgtaaatctt tgttttgagt tgcaactcct
    12001 actggacatg tatctttatg gcatacacgc atcataatac agcccaacac cactaatggt
    12061 gcagttgcaa atccaaattc ttccgctcca agcgcacatg cgtacgctac atctttacca
    12121 gttaataact taccgtctgt ttctaactta acacgacttc ttaagtcgtt tagttttaat
    12181 gtttgatgtg tttctgctaa accaatctcc caaggaacac cggcatgctg aatactcgtt
    12241 ttaggtgaag cccctgtacc accatcgtaa ccactgatga caattttatc tgcaaatgct
    12301 tttgccaccc cagatgcaat ggtaccaaca cctgttttcg aaactaattt taccgcgata
    12361 tctgcatctt tattcgcatt ttttaaatca tgtatcagtt gcgctaaatc ttctattgaa
    12421 taaatatcat gatgtggcgg tggtgaaatc agaccgatac ctggcgttga ccctcttgtc
    12481 ttcgcaatcc acggatatac cttagtacca ggtaattgac caccttcacc aggctttgca
    12541 ccttgcgcaa ctttaatttg aatttctttg gcatgttgta aataatcact agttacacca
    12601 aaacgcccag aagcaacttg tttaatcgca cttactttgt tgcttccatc aacttgtact
    12661 tcataacgtt ttgcatcttc gccaccttca ccactattac tctttccacc taattggttc
    12721 atggcttgtg ctaacgtttc atgtgcttcc gctgaaatcg atccataact catcgcccct
    12781 gtattaaagc gtttgacaat gtcacttacc ggttcaactt ggtcgatgtc aatcggtgta
    12841 catgctttaa attcaagtaa atgtctaatg tgatctgttc tatttttgtt caccgcttca
    12901 gagtatgctt taaattgcgc atagtcattt tctttacatg cgtgctgcaa taagaaaata
    12961 gattccggat taaaagcatg atgttgacct tgttgtctcc attggaatgt actacctgat
    13021 gcaagataat tatcatcact ttgttgacgt gctttatttt cagcatcaat ttgatcaatc
    13081 gaaatacccg ataacttaga ctgtatccca gtaaaataac gatcaatcac atcatgagac
    13141 aagccaatcg cttcaaatat ttgtgcacct tgataacttt gcactgtcga aattcccatc
    13201 ttagccatta ctttaatgac accttcggac aatacatccg tatatgtctt aacattatcg
    13261 acaacggtgc cttgtaaccc ttctgtcaat gtcagttgtt caactgtacg ttgcgctagg
    13321 tatggcacaa ttgcattcgc gccatatgcg agtaaacaag caacatgatg cacttctcgt
    13381 gtctcaccag atttagcgac taaacttgta gacatacgta aatctgcttt aataagtaat
    13441 tgatgcacat gacttattgc gagtaacatc ggcattgcaa agccattgct atcaactaat
    13501 ccactatcat ctaacactag aatttgagcg ccttgcttta cagcattcac tgcttctcgg
    13561 cctaatgctt ctaacgcatc ttccaaatcc ccttcatata ccgttgataa ataagttaat
    13621 ttaaaatgtt cctgatcaat cgctgctatg tgtgattcat tcaataccgg ccttttcaat
    13681 tgaatacgat ctaaaaccgt ttcgtcaggt gctagtaagt taccttcgcc acctaaataa
    13741 gaaagttcac tcgttacgat tttttcacga tacgcatcaa ttggtggatt cgtaacttgt
    13801 gcaaacagct gtttaaagta attaaatagt gattctggtc gctcgttcaa cactgcaatt
    13861 ggcgcatcat atcccattgc accgatagga tccttcttac cttctacaag ttcctgaata
    13921 tacttatgaa tctcttcttt cgtgtatgca aactgacgtt gtaatttaaa taacgtctca
    13981 tctttccatt gcgaatcttg atattgtata ttttcaaaat caaagtcaac tttatggtta
    14041 tcaatccacg ctttatatgg taattctcca gcaatcgcac ctttcaaatc attattttca
    14101 atgactttat tctgtttaaa atcaacaagc aataactttc caggattcaa ttgaccttta
    14161 aaagcaacat tactttcagg tacgtccaca acacccactt cagatgaaaa gacaataaag
    14221 ttatctttag taatcgtata acgacctgga cgtaatccat ttctatctgt aagcgcgcca
    14281 agtttgtcac cgttacagaa cgaaatcatt gtaggaccat cccacggttc cattaaataa
    14341 ctataaaatt cataaaatgc acgtacattt gcatcattcg cttcattgta taaccaaggt
    14401 tcaggtatga gtaacatcgc tgccttttct ggctccatgg ctaacgataa gaactctagc
    14461 gcattatcta caatggcaga gtcactacca tcctcatcga caatttgaaa cactttatgt
    14521 tgatcctcgc caaataatgt ttcgattaat ttatgttggc gtgctcgcat ccagttcaca
    14581 ttacctttaa tcgtgttaat ctcaccatta tgcattaaca tacggttagg atgcgccctt
    14641 ttccaactcg ggaatgtatt cgtactaaat ctcgaatgca ctaaccctag ctttgattga
    14701 tataaatcat ccgataaatc tgtatagagt tttttaattt ggtctgatcg taaccaacct
    14761 ttatatacaa ttgttttgcg tgataagctc gtaaaataca attctaaatc gcactgagtc
    14821 gaatagaact ctaattgttt tctcgctaaa aacaaacgct tttcaacatc ttcaatgtcc
    14881 ctaatatcaa taaacacttg ttgaatgact ggcatcgtat ctgctacatg tttagcaatg
    14941 gcatctttat taactggtac attacgataa ccaagaattg ataacccttc accttcaaaa
    15001 tattttttaa aaactgcttc atgttcagaa cctaaaatgc gttctttgga aaaaaataac
    15061 cccacggcat attcaccttc acctgggata tcaaagtccg ttacatgttg tttgaaaaat
    15121 gcaaaaggta tttcagtcat aatacctgcg ccatcaccag tgatgccatc tgcgccgacc
    15181 ccgcccctgt gatctaagcg tcgcaacatt tcaagcgatt tatcaatgat gtcgtgagac
    15241 cttttattat ccatattcgc ataaaaacca ataccacacg catcatgttc ctcacgatag
    15301 tcatataagc ctttaattaa tttctcattg tgcatgatgc acaccccttt ttttagaata
    15361 ttctgatatt agttattaat gataattact ataatctaaa aaatttatct gttcaatata
    15421 taaaatagaa gatattcatc taaaaaatag aacatttatc aataatattg ctataatagt
    15481 tacaaaacac tcatttttgg aggtgcctat atcactatgg aaattaaaca attaagatat
    15541 tttatcgagg tcgctaaaag agaacatatc tccgaaacag cacttgaact aaacattgct
    15601 caatcagcga taagccgtca aattacatta ttagaacaag aacttaatgt gtctttattt
    15661 aaaaagcaag gacgcaatat tacgctcact tctgaaggga aattattatt taatgaagct
    15721 ctgcgcatta ttgaacattt agatagcacg attgaacaat ttcaaagcca tggattaact
    15781 aaaaataaaa gtatctacat tggctatgat gagagtgacg tatcccacat gttactacca
    15841 cttatccaaa cgtttcattt acaaaatgat acacatgtca taccgaactt attagatcat
    15901 gacacgatta taaatagcgt attaaatggg aatatagaca ttggtttcac ggaacttaca
    15961 ccagaaatac gtaaacacaa acaattacat atgctacctt tattcgaaga gcactatcat
    16021 ttatatgcgc cttcagacga tccaattacg atggcgacac acccaccatt aattcagttt
    16081 gagcatagtc atatttattg cttggcacca tttgccgaaa ctgtaaaaaa acaattgcgc
    16141 aagattacta aatcagatgt atacacaatc agtagccaac cacttgcgca gtatctcctt
    16201 cgtcaaaaag aaggttacat catttcatca caaaatatac atttaccaga atctaaatca
    16261 tggatagata taaaattaga tcatactgaa ttaaaaagaa cgatatgtgc tattacgaaa
    16321 gaaccttata cgaagagtga tattggtatc ttattaacac tcattcagca attaatgact
    16381 aaaacgtcta cattccatta agtaattcga tgctatcaat gatgattttc gttttacact
    16441 tagcagtaat atctttgaaa ataaaaaatt aagggcgtat aatcaccata ttaattcatt
    16501 catagaattg gggttttgtt tttgaaatat agaaatttat ttacaaagcc atttaatata
    16561 attttaggtt tgttctgcat cgcattcatt ggtgctattt tatttacatt caataatgag
    16621 aacttttaca acaaaccaat tggtcaaatt atagatgtca aacatgtttc ttcgacacct
    16681 acaaaagatg cacaaaataa tcgtgatatt aaatataaaa atcaattaaa agtcaaaata
    16741 ttaaatggtc aatttgctgg agaaacgacg acgattaatc atcagtacgt caaatcgcaa
    16801 gcagattctg aggcatttcg cacacacgaa aaagtattat tacatatttc taacaagccg
    16861 agtgatgctt atattataga aaagaagcgt gataccttaa cggttatcat cactggtttg
    16921 ttcttattaa ctgtattatt ggttggtcgc aaagtcggac tgcaatcgat tttatcgtta
    16981 atcttgaatt ctattgccat attaatcgca atttacattc atattcaaca tagcaatatt
    17041 aatttattct tactcatgac cattgccatg atctgttcaa caatactcac tttattgctc
    17101 gttacaggct ggcatatgcg cacactcatt accatagcca gtacaattat cggaactttt
    17161 ttgagtatag gtttaacaga attaatcata tatatgacag acggtaaagg cattaaatat
    17221 gaaacgatga actttttatc attaccaccg aaagatatct tcctagcctc agtgttaata
    17281 ggttcacttg gcgctattat ggatgttgca attacaattg ctagtggtat gcatgaaatt
    17341 ttacagcgta cgccacacat ttcaatgagg agatgggcgc ttgctggccg aaacatcggc
    17401 caagatatta tgggaacaat gactaacatc ttattattct catatttatc cggtgcacta
    17461 cctatgtttt taatcttttt aaaaaatgcc aatacagtga cttatactat ttcgatgaat
    17521 tggtcgttag aaattgcgcg cgcactaaca ggtggtatag gtatcgtact tacaattcct
    17581 atcacaatat tgtttatgga aatatttgaa acattaagga gggcgaaaca atgaatgctg
    17641 tatttatatt ggcgttattg ctcctctttc tgatgataat tttcggaggt agaaagggat
    17701 tcatctctta tttaacccta tttttaaatt ttgctatttt aattattagt attgtgttga
    17761 ttatttttgg cgtaccgatt tatgtcgtca cattcttttt ctgcattatt attggcgctt
    17821 gtaatttatt cgtactcaat agttacaatg ttaaaacaca agctgcattt atcagcacta
    17881 tcgttacaac aatattgttg attacactca tttatttatc ggtctacttc ggtcatctac
    17941 aaggcttttc aaccgaacaa caagatgaaa catatgtgta ttcaatgaat attggcattg
    18001 atatggttca atttatggtg ttcactatta ttcttgctgt tattgcagct gtgatagatt
    18061 taacaattac cattagttca cccatttatg aattgcatca agtgaatcca acgcttacgc
    18121 aatacgaatt attccaatct ggcatgcgtg ttggtagaga aattttagcg acatctgcga
    18181 acacgattta ccttgccttt tttggtggcc aacttgcttt atttatttgg tttttcaaac
    18241 ttaaatattc atttggtcat attataaact cgaaaatatt tgcccaagaa tttatttcta
    18301 ttattttagg cggtattgcc gttgcaatca gtattccgat tacggcttgg attacggcat
    18361 ttttaataaa aagaccatct cgacagcgca aaaatgatga gtctatcaat taatcgcaac
    18421 tagcaatata acttaagtta agcgacctgt ggatatatgt caattgttca ctaggtcgct
    18481 ttttactata gtaaattgtc cgatgatcta taacaaaaag cacctttatt catcattaat
    18541 tgttgtggtc gacgatgagt aaagatgctt ttaaatttaa tttattttat agttaacgcc
    18601 ataatatgaa caggtcttcc gatgaacata ctatctccca tatattgata tccttgttgg
    18661 cgataaagtg ccatggcatg tggattgtca gtattaaccg ttaacacaat ctcattaata
    18721 tcctgaaatg ttgaagtgat aaatgacgcc aatttttcca ttaccacttt acctattcct
    18781 ctattacgat aacgttgatc aacactaaat gacctgaaaa atactgcatc ttggttatcg
    18841 ctaaatggtg cgaccccttt tccacgatgt aatgtaaaaa aggccacaca ttgcctgcca
    18901 tccattacaa cgatgggata cctttcatta tcagacattg cagctgtaat attttcctct
    18961 ggcgttttaa caaacttcaa atcactttca cttaattgaa aagcttctaa catcgaataa
    19021 tcttgtggga cataatgtcg taacatgatg gtgtcattat ttgtcatgtt taccaccata
    19081 cgtttcaatc caaactttga cagcaggcgc aatcaacgca tcattatctt tatcaatcca
    19141 gcgtatatcc gtaatttcat tattgatagt tacgttttcc caatcgattt tggttaatgc
    19201 gcgaaatcca tttaactcag ttaacatatc ctgttgtgga tatgcaggac ctacaattgt
    19261 cccgatatat tcaatttcat ccattgttaa tgttaaattt aattcttctt ttacttctct
    19321 taacagcgct tgtactcgtg attccccttc ttctatttta ccacctggga aataatactt
    19381 ttcgcgattc cttacttgaa caagtaatat tttatcagct gtttcttcaa ctaaacagac
    19441 acatttaatc atctttgaca ccccaacttt gtgaaatcaa tttttaaaat tatactgtac
    19501 aattatgtta tcatatatga gtagttatag cgcaaaacgt tagcaattca gcgcacccaa
    19561 tttttcatat aaacagaaga tactaggggg aattattatt atggcaaaac gttccaaatc
    19621 acaacgttta tcaagtttac taaatgtcgc aggtttcata gtcgacggct acaatggcta
    19681 taaatatcat gctaaaaata aaaaattagt atatctttca ttaggtttaa gcactgtagg
    19741 aaccgtgtta gacttttaca tttcaattaa gtcaccacgt aagttcaaaa aagcagtggc
    19801 agttgttact ttaataacaa acggtgctag attatttaca agcattcgca aagtaaagca
    19861 tgaatactaa ttcagaaaag gattggtcga acatagaaca tgaagttcat tcgaccaatc
    19921 ctttttctta tacaaaaatt cctattacaa caatcacgac tttgatagcc ccgccataaa
    19981 gtttaggatt caatccaact tttcagcttg tgaaatgtaa taggaattca ttatatattt
    20041 atatacgtaa gactttagtg aatatatcta taattattta cttggtaagc tggtaccgtt
    20101 ctgtaagtta aaatacctgg tgcagctgaa tagttcattt ctgaaactaa aatactacca
    20161 tcgttattta cacgttctac aaacataaca tgaccataat aacctacatc tgtttgagcg
    20221 atagaaccta cagtaggtct attgtcgata gtgtaaccat ctgctgccgc tgcgttatcc
    20281 cagttattag cattccacca ataagtacta atacctttac caatttcagc acgacgatta
    20341 aatacatgat atgtacattg accccatgta tataagtttt ggtgactgaa tactggtgta
    20401 ttgtaacctc tatttgtcgt tgttgcagat cctgagttcg tagatgcatt accagttact
    20461 ttcaattttt gacccggata tataaagaaa ttatttaagc catttaagct cataattttt
    20521 tgataagttg taccatattt tgatgcgatt aatgacaatg agtcacctgc ttgtactgta
    20581 tagtatgatc cgccacctga gttcgttgat ggacggctac tattgctcgc agcgttactt
    20641 gagctagcag tacctgatac ttttaatttt tgacctggat aaataaagaa attatttaaa
    20701 ccattaagtc gcataatgtt ttggtaagtt gtaccatatt ttgatgcgat taatgataat
    20761 gagtcgcctg cttgtactgt gtagtatgat ccgccacctg agttcgttga tggacggcta
    20821 ctattactcg tagaattact tgagccagat acttttagta cttggtttgg gaaaattaga
    20881 ttagatgtta aattgtttaa tgactttaat ttagcaatcg aaatcccata cttatttgaa
    20941 attgcccaca ctgattcacc cggttttact gtgtgagttg tagccgcatt tgcttgagtt
    21001 gccgcaacag cgctaatcgc gcttgtccca ataatagctg caattacttt tttttgcact
    21061 ttaaaatcct cctcttgctt aactttccta acattctttt atccgaattt atgaatacta
    21121 catcattata cgattttatt atgtataata ggttgatgtt tgatgacatt atgattacaa
    21181 aaaaatcata tactgtatca tcaaatttta taattacccc ttaaaattat tacaacttat
    21241 tagattttac aatatctaaa ttattacaat ttcataatat ttcactataa aatgattaca
    21301 atccctttct ctattggaaa taattttatt ctccaacaat aacgccctac aaacataagc
    21361 atgaactttg cttgtaggac gcaatataat ttattttgct aatgacgttt ctattgcctt
    21421 aatctcatct ttagataaat taacaggttt ctctccatct ttgacatctt cctgcaacgc
    21481 tttttgagct tcttttgaat gatacaattc tacgatttta gcatatgttt tgttatccaa
    21541 gtctttgtca ttaactgcaa caatattaat atatggcttt acagcatctg aatttgattt
    21601 ttctaaaaat atcggatcat ttttagggtc tttccccgct ttagttgcta caccgttatt
    21661 aataactgca atatcgacat cagataaagc acgcgcagtt tgctgtgcat ctactgcagt
    21721 aatttttaaa tgttttggat ttgacgttat agctttcact gtacctgcta atccgaaatc
    21781 ttttttcagt tttattaaac cagctgcttc taatagttta agtgcacgtg cttggtttga
    21841 tacatcattt ggaatgacaa ctttagcacc atctttaacc tttttgacat ctttaatttt
    21901 atctgagtaa atgcccaacg gtgctaaaac tgttgtactc aatgctgaaa tctttgttcc
    21961 tttatgtgcc ttcttatact gatctaaaaa tgcaaaatgt tggaatgcat tcatatcaat
    22021 atcaccatcg tttaatgctt tattcggtaa attgtaatcg gaaaagtgct taatctccac
    22081 atcaatatca tcttttttag ctaattcttt aaccttctcc caagccttag tgtcatttga
    22141 tgcgacacca attgttactt ttttatcatt gttactacca cacgctgcta ataatacaag
    22201 tgctacgata actaacccaa tcaatctttt cattctatca attcctttca aaatcttcac
    22261 tatatatcat taatgtctac gtatgaatct agctaggaca ttccctagcg tttgaatcac
    22321 ttggacaata atgactaata caataacggt aataataatg accgtcgtat caaatctttg
    22381 ataaccatac actaaagcta agtctcctat accaccaccg ccaacagctc ctgccatcgc
    22441 cgtacttcca ataagtccaa taatcgcagt ggtaattgct aatactaatg aacctaacgc
    22501 ttcaggaatt aaaaaatatc taatgatttg tagtggtgaa gcgcccatcg ctttcgccgc
    22561 ttcaataatc ccctcgtcta cttctaataa tgagttttca acaagtcttg caatgtaagg
    22621 tgccacatat actgttaaag gcacgatggc agcagtcgta ccaattgaag tacctactac
    22681 taattttgtg aatggtacaa tcgcaattaa caaaataata aatggtagtg accttaaaat
    22741 attgattata ggatttaaaa cttgatgtat cactatattc ggccatatgc cttgttttcg
    22801 agtaattacc aataagacac ctaatggaat accaatcact gctcctaaaa ataaagcaat
    22861 agataccata tatagcgttt cgtacaatgc ttgtaataac tgtgcactgt ctaaatcaga
    22921 accaaacata tgttaatgca cctcctcaaa ttgaatattt ttctctttga aatattgatt
    22981 tattgccgtg tcatcaaatt gttgatccat attaaatcga agccacatat aacatacggt
    23041 gttaccttgt atttctgaca tagatgaaaa taaaatttta acctctctgc cacaaatttg
    23101 aatcaagtca tttataatcg gttgtgtcac ctgagtttcc tcgacgaaga ttttataatc
    23161 tttaaaatcg ccaacttgtt cgtcattcaa tcgacgaatc aatgatgtac ttggctcagt
    23221 ctgtataact gtagacacaa aattttgagc aatcgtcgtt ttaggatgac taaacacctc
    23281 tttaacagtt cctgtttcaa ccacttgacc cttttccatt acagcaacac gattacaaat
    23341 gtctttaata acgcgcattt catgtgtaat catcataatt gtaatgccaa aggtttgatt
    23401 gacattcttt aataacgtca atatcgaagc agtcgttgct ggatccaatg cgcttgttgc
    23461 ttcatcgcat aggagtattt tcggattagt aacaagcgct cttgcaatag ccaccctttg
    23521 cttctgccca ccagataatt cttcaggaaa ttggtctttt ttatcactca atcctacaaa
    23581 ttcaagcatt tccgttactc gttgcttaat ttctgttttg cttttcttac ttaaaatgag
    23641 tggcattgct acatttttaa atacggtagc tgaatttaat aaattgaaat gctgaaatat
    23701 cataccgata tctttcttaa tatccctcat cattttatcg ctataattcg taatatcatg
    23761 tccatctaca atcacttgtc cattcgaggc agcttcaaga tgattcacga gtcttaccaa
    23821 cgtacttttt cctgcaccac tatatccaat cacaccaaaa atatcattgc gattgaccgt
    23881 aaatgatacg tccttcaaag catctatttt ttgcttcttt ttattaaagg tcttacttac
    23941 ctgtcgaaac tcaatcatgc aaagccccct ccttcaaatt cttatcattc cactaggaat
    24001 aaaagcatta tatgtaaaat tgcatatatc gtcaatacaa tttgccgaat tttctaaaaa
    24061 attaaaaaat aagcaattca tgtgacaatg acgaattgtg agactactat gacatttatc
    24121 aaattaaatc catacaaatg tccaccaaac ctccataact caatcacgaa tgattaacat
    24181 cgcacaaaaa aagcactagc atattcaaga acaacaaacg ttgaactcaa aatatatgcc
    24241 agtgctgcta ttatttataa cgtatctagt gcttgtttta aatcatcgac taaatcttca
    24301 gtatcttcaa tacctacaga aattcttaca agtccgtctg taataccttc tttagctcga
    24361 atatctgctg gaatggatgc atgtgtcatc aatgcaggta ctgaaattaa actttccact
    24421 gcacctaaac tttcagctaa tgtgtaatac gatgttgctt taatcaattg tttggcactt
    24481 tctgtatttt tcacttcaaa tgcaatcaca cctgtatggc catccgcttg agccatatgg
    24541 acatcatgat ttaaatgact ttcaatactt ggatggaaca cttgttgcac agctggatgt
    24601 gcttgtaaca ttttaataat ttcaataaca ctgcgattaa tttgttccat acgtaaacct
    24661 aatgttttaa tacccctcac aagtaaatag ctatcttgag gtcctaaaat gccacctgtt
    24721 gaatttgaaa taaatgctaa acgttctgca agcttgtcat ccgatgttgc aactaaacca
    24781 gcaacgacat cactatgtcc acctaaatat ttcgttgcag aatgcaagac aatatcgatg
    24841 cctaaatcta atggattctg ataataaggt gtcataaatg tgttatcaac gactgaaatc
    24901 aaaccgtgtt ctttcgcaat ttcagcagac tttttaatgt cagtaacacg taataatgga
    24961 ttagaaggtg tttcaataaa caacatcttt gttgttgggc gtatcgcttg tacaattgaa
    25021 tctgtatgcg ttgtatctac aaaatccact tcaatgccaa atcgtgtaaa tacttttgtc
    25081 aatgcgcgat aagtaccgcc gtatacatct gaatttaaaa taatatgatc acctttgtcc
    25141 aacagcataa caactgcact gatagctgca acacctgaac taaacgcaaa gccatgtttg
    25201 ccattttcta atgccgtaat aacgctttct acagaacttc ttgttggatt cgcagtacga
    25261 gaatattcat atccttgacg taaatcacca atatcatctt gtaaatatgt acttgtttga
    25321 taaattggtg tagtaactgc acctgtatag tcgtccgttg tgtgtccacc gtgaattaat
    25381 ttagttttct tattcataat cattctcctc ataattaaat atttgcttag acatatatcg
    25441 atcgctacca tctggaaata cgacaacaat cgtaccttca gataattgcg cttttaaatt
    25501 caatgcacct tgtaatgctg cacctgaaga actgcctact aacaaccctt catttatagc
    25561 caaacttttg acatttcgaa aggcatcttg atctttaatc gtaaatatcc catctacaag
    25621 acgtctctct aaaaatatcg gccatttctc agaaccgata ccttcagtgt catgtgcatg
    25681 agctggccct ccatttaaca cggacccttc tggctcaacg gcataacatt gcacgtgatg
    25741 ttgctttaaa taacgtgcgg tacctgtaaa tgtaccgcca gagccaatac cagccacaaa
    25801 ataatcaatt tgctgtaatg ctgaagtcaa ttcgggtccc aatgtatgaa aatatgtatc
    25861 cggattatgt tcggattcaa attgattcat ataaacggca ccatattttt cagcatagga
    25921 acgtgcagct aattgtgccc catgcatacc ttcagactga ctcgtccttg aaacatctgc
    25981 accaagcgct atcataatat taatcttttc ttctgaaaaa ccatacggcg caaagatctt
    26041 acattttaaa tgatgtctat tcgctgcaat agctaaccct atgcctgtat taccagcagt
    26101 cgcttcaaca atagtttgac ctgcacgcac acgcccttct tgaattgcct tctctactaa
    26161 atatttcccg agtctgtctt taacactgcc tccaggattc cattgttcaa gcttggcata
    26221 aattttaact ttatcatcac tataatgttc taacagtact aatggtgtat tgccaattaa
    26281 atcataagta atcatagatg caccctcatc tgacatgccg atcaaatgaa tgaaaccttt
    26341 cttcatgtct caattttaat tcttactttt cagataagaa ttataaacga cattttgtta
    26401 ttttgcaatt atctaagttt cgattaattc agaaccagta ctaacttttc aattccaaac
    26461 aaaaaaacac ctgagcaaca caaatacttg tgtgtcagat gcttctatat attaactaaa
    26521 taattgcacg ataaagacta aaataataac gacaggcatc gcatacttaa ttaagtaata
    26581 ccaaccactg aataatctaa atcgatcttt accaaaatat tgttgtaata attttttatc
    26641 taataattgt cctacgacaa gcgtagtacc taatgcgcct aatggcatca atacattcga
    26701 aacgatgaaa tccatattat caaaaatcgt tcccgcaccg aatcttacat cttttaagat
    26761 accaaaagat aaggttgctg gaatactaat gataaatact aaaatactac cgatcactgc
    26821 gactttttta cgttttgtat tgtcattctt cgtgaagtta gaaacattta attctaataa
    26881 agaaatagat gacgttaaag ccgcaaataa gaacagcact aagaatccca aatagaataa
    26941 tgtgcctaga tgcatttgac taaagaccat tggcagtact ttaaataata atccaggccc
    27001 ttcttgtggt tcatagccaa aactatgtaa cgccggaaat atagctagac ctgccaatac
    27061 agatacaaag atattcataa caacgataga aatagctgat gacttaatcg tcatgtcttt
    27121 agaggcataa ctcgcataag taatcatacc tgtagttcct aatgataacg taaagaatga
    27181 ttggcctagc gcaaacaaga taccgtcagc agtaatctct gacactcttg gttgtaaaat
    27241 aaatttcaca ccttctaagg cgccatctaa tgttaaagac ttaatcacaa tgatgattaa
    27301 aaagacaaac agcaatggca tcataacttt cgatgccttt tctaatcctt tttcaacacc
    27361 taacatgaca ataatcatcg tagcgaatat gaatatacct tgccctagaa cagttaacca
    27421 aggatttgat attaccgctt caaaattcat ttcttggaga tgattgatgc gttgaaatat
    27481 aactaattgc cataatactt gtccgatgta aatgacaatc caaccaccga taacactata
    27541 gaagccaaat aaaataaaca cggccaaatt accgttccag ccaatgatat tgagccattt
    27601 tttaccagtt aatttactat atatttgtgt tgtatatgtc cgtcccattt tcccaacagt
    27661 gaattccata atgagtaatg gtaacccaac aaaaatggtg aatattaaga acatagctag
    27721 aaaggcaccg ccgccataaa tccctgccat atatgggaat ttccacatgg caccaagacc
    27781 gattgcagaa cccgcactag ctaaaataaa tccagttgat gacttccatt gtgattgttg
    27841 tcttttcatc attcacatct cctttaaagc gttaaagcgt ctacgtgttt aagtaacact
    27901 ctaacatgta tgtaaataac atgcaataca cctgcatcaa aattgtttca ttaatacggt
    27961 agcgcctgga tttatcataa tatagtcaac tcttgtaatg tgttgtcatt acgctacatt
    28021 aaaattcctg gttcaacgct gtatacgtgc gccaatatct cgccacatac aacaccatct
    28081 atatccccaa catctcgcca cataataact tcaggtgcaa tgcccataaa atgcccacaa
    28141 cattacaata caaatcaact tctggtataa ctccataata tttccacgac atcacactaa
    28201 aaattaactt tagctaagtt ccctatacta tcacgccaca aaaaacactc aaatgacaaa
    28261 tataataaca tcgtcactca agtgttaatt tttaaaatct tcaattatgt taaagcattg
    28321 aaaaagcgaa taacattttc ttctaaaata tctctgcctt ctccatgcgt cattaaatga
    28381 tgcgaatcct tcagaccatt taattctttg tctactgatc ctaaatgttc ataaataaaa
    28441 tgtgcacttg tttcatagga tggcgcatct ttaccaccaa ataatatatt agctggcatt
    28501 ttaataacat ttaaatttgt catgatatca tcaataaaat gttgaaactt cgtaatttca
    28561 ccttcataat catcaattgc tgctaattgc gcactcgatg cttgctgatc taaatttaaa
    28621 atatccgaca tgcgttgact ataataaact aaatgttcta ttaaaccatc gtcactcttt
    28681 tcctttggtg ctgacatgac agcgatacgt ttcaaaggat agtgttgcgc caattttaat
    28741 gtcattaatc cacctaaaga cacacccgtt gcactgatag attcataacc ttcattgact
    28801 aaaaattggt aagctttctc aacttcttcc caccaatcat ctacattata tgtcatgaaa
    28861 tctttcaaca gtaagccatg acctggataa ttcggtgcat aacaactaaa tccttggtca
    28921 tttaactcag ctgcaagatg cttcacatcc cgatttgtac ctgtaaatga atgtaataat
    28981 aatatcgcat gtccatttgt gccttttaaa taagagggac tcggtgtttt aattctcatt
    29041 tttctatata cctccactat gtctaaagat gtttgctaaa cgcgttgtcg tcgatgatta
    29101 ataaagtatg ggtataccat taagaataac gctacccaaa tgattgctag tgacgtgccg
    29161 ccaatgacat ctgaaaagta atgcgcatga aaataaaggc gacaaaataa tatgctaagc
    29221 cataatattc ccataactag cgcactcaac acttttgtta ttgtcttagc agcaagtgaa
    29281 ataattatga tcattaaagc gaaatataat aatgtgctgg cgttggaatg tccacttgga
    29341 aatgaaaagc ctgtatcaac ggctaaatga ttatatggtc ttggacgtaa aacagtatct
    29401 ttaattaatt tgttcatgat gacacctgaa accaaatatg tcacaaacca aaccgctaaa
    29461 tgcctctgtt taataaacag tatgattgtg acgataatgg aaatcaacac gacacctttg
    29521 acatctccaa tttccgcact aaacgtcata tagtaattaa ataaattgtt aacatactga
    29581 cgttgtggct caccgaaata atctgtaaac catgttaatg atcccatatc tatattttta
    29641 agccattctt gatttgtcac tacactgtaa aacataccta taaatacaat cagcgcgatt
    29701 aaaaataaag gcactgtcat tttcggtgat gttaattttt tatctatcat cttacaatct
    29761 cctcgtatca tcattttcat tttacaaatg ttatccataa tatcaatgtg ccacaaattt
    29821 cactttaccg acaatatcaa aattataaag ttcatattgt tatgtatatt gcaaataaaa
    29881 cattgtataa ttgaaataac aatattttgc tattttcaat ttagtacgat ctatatttat
    29941 tatacagagg gggtaaggcg tatcaataga gttatttttg tctatatagc gttaatcatt
    30001 acattagtta gattctttac ccctattcat ccatcgtttt caaatttgat ttactggata
    30061 tttgtattat attttattcc tattatacta tgcgttatcg gtttcaaggc cgaaaaactt
    30121 attgcaacaa tggtcattat acctaatttt ttaggaatac tttatcgatt atatgcctac
    30181 gtcacacata tactctttat gtaaaaggat agtgggcatg tctcgcaaca aatatgcttg
    30241 cgtcgacttg tcaccattcg caaattttat gacatacgcc tttcacgggc tatattcaga
    30301 cccacgcatt catccacgta ataaacacat catgtaatag aaaaacagca cacccaaata
    30361 tatggcgttg cgctgtttaa ccaagcatac ttctatagct ttaataagcc agcaaaagca
    30421 tacctaacct tcttaaatat gcttttccaa attatcctca agtttgaata cgataatacg
    30481 ttcacctgta actgtactta aatcactatg gaagctcatc actttgatac ctgtaatttt
    30541 aaaaatgata tcattcaaat cttgctcacc ggattcaact aattcagaac gtgttcgttt
    30601 aatatttagt aatccttcat tcgtactaca cacacgatat tctgctggcg ttaagatacc
    30661 ttgtaaacta ataatcacca tatctcttaa aatgtctgat ttaactgaca aggaacctct
    30721 gccaaggaaa tctttttccc attgcgtaat ggctttactg atttcagctt cgatttcacc
    30781 tttcgttctt ttcatatcac taactccaat aatattaaaa ttgattactt catctttgta
    30841 tcgttatcac gacatcaact attacattaa gtttatcatt tttagtatat tttaagaagc
    30901 tagaacattg tagatatgat gatatattag ttacttagca tcgcaacata tcatcgttaa
    30961 atccaacttt taaaacgccc ttcctcatta acgctcatta aacgcagcca atgattagac
    31021 accttcctag cgaaatggtc attattcgcg agtagtcttg ctacaacata gtcgggtgcc
    31081 tgaacaacga caagcaaacg aattggcgaa tgatacatcg ttcgatcagc agccataaca
    31141 gattgccatg ataagccata catcagatca ctcgcattac cttgcatgac accaacacct
    31201 gacgtgacgg tttgtgtcgc tttattccca cttccgtaaa aatgcggcgc aactgtcgac
    31261 gcataatatt gtaaattaat ccattgtgcc acaagcgccg gaccagaaat gatagtattt
    31321 aacaatttgc catctttatc tttacgccaa tcataattgt gtaaaaatgt ccgcccttct
    31381 aaatcaatgc cttttgttaa ttggcgtcgt ccaattataa atgatgcatt tttagccaag
    31441 ccccattctg gacgtacctc actccaatca ctcgcaaacc gctgcgcttc ttccactgga
    31501 tgattcacac gaccaatcgt tggcagtttg tccaaacgtt cgcgattcgc gtgttcagaa
    31561 atcatcggca tcgcgtcatt caatgattca tatgcatcta aagcaataga agataatgtg
    31621 tctggcacat atacccatgc caacgtatca gtagacgtat gatgttctgc tgccgcaaaa
    31681 acagttgtct ctggaatata cacacctgat tgttttaatc cttgtctgac atttggacga
    31741 ttacatatca tcgctaataa cttagcatta aaaccgcttg atgcgccacc acaagcccca
    31801 cattcaagtg atgcatgatg tggattattg tgagaatgac tagcatgacc tgctaacaca
    31861 acgaacggcg caaatgcttc ggttaaatcc atcaatttca acgcttgtaa cgcgaaatca
    31921 atttgctctt gctcagtaaa tccaacaggt aagtctgatg ttcggtcaaa ctcacgatca
    31981 atcgttaact ttgtttcagg ctttttcaac catttttgtg ttattttttg taaagacgcg
    32041 cgactttttc taggcataat cgaattgaca atggtactca agcttaaaaa tggcccactt
    32101 aattcaggca ataacagact aggcataaca ttatttttca tcaatttaaa tgtgtaaaac
    32161 atcgatgaca ttgtctgttg ctgttgtcga taaacattca tatcgtagcg gtctgcaaat
    32221 tctttaatgc gatatgccgg cggtaccatg acaggtaatg aatcatgttt gaattgttcg
    32281 tctacggcat ctttttgaat aggtaatcca aagaaacctg caataccaat cgtttcaaag
    32341 ggccctgctg cttcgatatg tctacgaaat ggttctgaac gaacatctat acaaaatgca
    32401 atttgcgctt tcgttgatgt gcccacctga tttagctcgc tattattttc atcaactgct
    32461 tgtgtgtcat ttaataatac tgaatgtggc tgattagcgt tatcattttc tgagacattt
    32521 acttggttta catctaatgc gcccgccaca ctttcatgac ctgctttaat tttttgtttt
    32581 aactgagatt cgtatgtcat ttcccaggca attagccata aatttttaaa tacattttta
    32641 ttcatagttg ctgcaaaatg aataaacgtt tgaatttcat tgacgtcatg ttgtagtaat
    32701 acatcgctag gcatatcact gtagtaacac catgatgcaa cagtttgctt aaaccaattt
    32761 tccgatctac tttcacaatc tttagtgact gacttaaact catcaccaac tagcaattgt
    32821 tcgacaacta accgaattgc caaataatcc gttaacaaat gttgttcaaa gtgatgctgt
    32881 tgtgaacggt aatacaacat acctgcccaa cccggtaacg ccaaaagatg tccttcaaca
    32941 taagcttggt agtcttcctg atctattgaa aaatgagtta atactgactc tatcgtcatt
    33001 ttaggatcat tgggcaagcc tttaatcact tggcgctgtg ctttagtaaa actatgatca
    33061 tgttgcgcta aatgcaacca tgcatggtaa aaactttgct cacgcttcgg cattgtccaa
    33121 ctcgatagaa attgatcgat ataaagtttc gtccatttaa tcatttgacg attcacttgt
    33181 tcgctaagtg gctcaccttg ttcatctatt attgcatcac tcatcggacg tacatcatag
    33241 tgatgatatg aatcagccat atcacgtttt gatttttcta atagtagatc agcaacaaca
    33301 tcaacatttg aatgattcat atatgatgca ggtacgtctt ttaatgtttt aatgttatca
    33361 atataaagat tgatgtagtg ttgcgggata ttgtagtgat gttcaagtaa catatcagta
    33421 acaagttgat taaagacact ttcgtctaat tcaccacgtg ccacagcact ttctattaat
    33481 gctttatttg gaaaaatatc cacatctcga acatcacgta accattttgc aacatcttca
    33541 aacgtatccg cttctaatcc ttcccatgga tttcgtgctg caaaaatcga aatcggtgat
    33601 aatggtgtaa taacacgttt cgcattttca atgactgaat tgatatttaa ctgtgttgtc
    33661 atacctttca cctcctataa atacttcttc aaataattcg gatgactttc tatcgctttc
    33721 gagcgtgctt cacctagatt aactaaccac acgtacaata ccgcaaaacc cttagagtat
    33781 cgatgacgcg ccacccaaat acttaataaa ctaccaaata ttaaaatgac aacactaatg
    33841 atgacactca ctgtaggcgg cgttgtcgca tgtgttgtta tattttgtaa tacatcgtaa
    33901 aaataattgt gtgtgatgac gtagataaat gtcacaattg caatcaaaat catgccaacc
    33961 agacgtgcca tgcgcccttt actaaaggct accatctgat tccaagatac aagtaatgac
    34021 catgctagaa tgagtgcact taacacttca tatgcacttc tgtcactact catccaaaat
    34081 agaaatgcca cgataatagc taatacacgt cccatgacaa tccagccata agcgtcttta
    34141 gcagatgctt gttttggaat attgaatcgc ttcacgatag aacctgattg taaaaataat
    34201 gttgctttaa aaataccgtg caatattaaa tgaataatcg ctgctgaata tacacccaat
    34261 gcacattgaa ctaacataaa gcccatttga ctcatcgtag agccgactaa ctgtcttttg
    34321 taatccactt gaaccaagct aatacccgat cctaacaata cagaaatact agaaaggata
    34381 agtaataatg ataacgcaaa tccattatca aatatcggcg caaaacgagt tagaataaca
    34441 ccacctgcat tcacaattcc tgcatgcata attgccgata ctggcgttgg tgctgttaca
    34501 gattcaatca accatcgatg aaatggaaat tgtgctgctg gtatcatgac agctaataca
    34561 agtagtacat tcgtcaacaa tgaccatgtc gaattagaca tatgttgagg cacgcgccac
    34621 tcgccagtcg caatataaat agttacaatt gctccaacga atgcaagcca accacataaa
    34681 aatgtcatac ttgataattt cgcagactca cgtggcactt tccaaaaacg attaacgttc
    34741 atcagcaatg ttaaacataa taatgtcata ccccagcaga gtgccatcag tcttaagtct
    34801 tcagacatcc atgctaaaga tgcaaacgac gtaatcgcag tgaacaatgg aaagtaatgt
    34861 ctataatgat gatcacctag taaatatcgc attgaaaact tttgaataat aaaaccaagc
    34921 gccattacaa agccagctaa taaccaagat aaacgatcta ttttaaatgg acctaagaca
    34981 tgttgaccat gaataccgaa aaagccaatg actgcaaata atactggcat gactagtatg
    35041 tataaatgta atttaatata tctcattggc ataactggtg ctaaaaacaa caagccactt
    35101 atcaatgcaa tgataagcgc aataacaaac agtgaaaata gcaattgaaa acttaacact
    35161 gcataacctc cttatttcta atctctcgca taattgctta tgtataaaat aaaaacctac
    35221 aatagtagat tctgtacata atggcagaaa atttactatt gcaggtttca gtttaactag
    35281 acactgcatc acggtacgtt gatatacctt gttgcattgt tctctttaag cgtgctccca
    35341 tgcacatatg tatataaaat gttacttctg tctgttcaat tcatcttcat aaatatgctt
    35401 tgcctagacg agacctaacg tgttattcgt tttaaactta taacataaaa tataattaaa
    35461 tttctgcttc atgtcaaatt catgagctta acctctatta aaccaatgat tgtaaagatt
    35521 ttgtaaatgc acctgtacag ttaggcagta tttcccgtcc ttttaaaata aaaaattcgc
    35581 agttatgatc ataacaattc aagttaggaa aaaaaatcaa ttacgcacaa gataactatg
    35641 tacaatgaag ttaactcata agcaaaggag gtaatcttaa tgggtatcat cgctggcatc
    35701 attaaagtta tcaaaagctt aatcgaacaa ttcactggta aataagattt cataacaaac
    35761 aaaggaggtc tttcacatgg gtatcattgc aggaatcatt aaattcatta aaggattaat
    35821 tgagaaattc actggtaagt aagttataaa aatctcatag atatgaacat cttatttgaa
    35881 gggggccatt cacatggaat tcgtagcaaa attattcaaa ttctttaaag atttacttgg
    35941 taaattttta ggtaacaact aatctcaaac attaacgatc aacaactcat cactatgtta
    36001 aatcaacata caggaggaca aaacgatggc tattgtaggt actatcatta aaatcatcaa
    36061 agcaattatc gacattttcg caaaataatt taagcgaatt gaatacttaa aattctcagg
    36121 ccactatacc aatagggatg gtggctttgc tttgtgttga tttattgatg tgaggtgagt
    36181 cttgttagtt tgttgcaaat aaatggtctt ggttttttgt ataggatgtt cttggtggga
    36241 cgtacggaat attcgtgatc tttgtagtct gacgcgttat atttttgtgg cgtgttttat
    36301 gtttgatact cgaggtctga gacattcatg atttggcata cgaaatctta atgattttca
    36361 taatctatcc caagatatat tggccacgtg cagaattgcg ttgcacgttt agactgaaac
    36421 actcgtgtga ccgtaagtgt taatagtaca ttgatagctg catttacttc actcattttt
    36481 atgactgtta aacaatgatt gtaccttcaa ttaacagttg gtacgatggt tttgccattt
    36541 ttcatcaaag caaacataaa aaggactaag acatatacat gtcctagccc tatggataaa
    36601 atgcaaattt ctgctttatc aaaactatca cactttagat agattgaaaa caaaaagatc
    36661 ctaagaacat cttaactttt tattaattgt cataaattgc aaacaattaa gccacaattt
    36721 aaaaatgatt atacttcatt caacttatcg tgctggtcta atttgccatt gatatggatc
    36781 ttcaaattgt tgccaatctg catcaatttc ttgcgcattg actaagcatg catcgagttc
    36841 ttttgttaat ttttcttcat ctaagtcggt accaataatg acaaattgtg tatgacgatc
    36901 gccatattct ggatcccatt cagctgcgac gtcttggcgt tctgctaata tttgcgtttg
    36961 ttgtgcttca gacatactag ccacccaata tgtaactgga tgaatattgc aagatgaccc
    37021 tgcttgagat aataaacatg ctacgtgatt gtattgtgct agccatacga taccttttga
    37081 tcgaacgaca ttatttggca tggtttctaa ccaatcattg aaccttttag catggaaagg
    37141 aagtcgacgt ttatatacaa acgatgatat accatattct tctgtttcag gtgtatgcga
    37201 tgcatgccca ccagactcaa gttctttgat ccatcctgct gactcgctcg ctttttcaaa
    37261 atcaaaacgc tgcgtattta agacttcttt taaatccact tcagaattta ttgtcttaat
    37321 aattttagca gtaggttgca atgcacttaa cactttttct aacttcgcaa gttcttcttc
    37381 actaattaaa tcaattttat taataatcaa tacatcacaa aattcaactt ggtcaattaa
    37441 taaatcagca atcgaacgct catctgtttc atcaacgctt tgatcacgat ccatcaataa
    37501 atcttctgag ttgatgtcat gtacgaagcg gttagcatcc acaactgtaa ccattgtatc
    37561 taaacggcaa atcgctgtaa gatcaatgcc aagttcatca tcaatatatg agaaagtttg
    37621 tgcaacaggt actggctctg aaatccctgt tgactcaata acaatttgat cgatgccacc
    37681 ttttttcact aaacgctcaa cttcttttaa taaatcgtct ctaagtgtac aacagataca
    37741 accattagaa agttcgacta atttttcgtc tgtgcgcgat agtcccccac catctgcgac
    37801 aagatcttta tcgatattga cttcactcat atcatttaca attaccgcaa tacgtcgacc
    37861 ttctcgattt tgtaaaatat gatttaacaa cgttgttttc cccgagccta aataaccact
    37921 taataccgta actggaattt tagccatata tataccctca cttcaattta tttgtttcgt
    37981 tcgtttgcaa ataggaataa ttccgtttta cattatatag gagcatttcc tctttcgcaa
    38041 tcttcgataa taaaaaaata gtatacttaa ttaaattatt gagcacttta ctttataatg
    38101 gagacaaaga tatatctcac gagagagaat cgaggtgtat aaacatgcta tttgtcattt
    38161 tagttttata tatcattggt attacattta ttctactcag tgtttttggt tcaaagactg
    38221 aaggattatc tacgaaacat actttatata ccattggcag tgctattata acgattgcta
    38281 ttttcatttc aattggctat gccattcaat acttaactgc agcgctttat ggtttgtaag
    38341 gtgaggatga tttatggcgg atagttcgag agagtttgac ttgcgttgat tttgataaag
    38401 aggtcatagc ttttggtact tgaggatttt gttgttgctg tttggaattt ggatatgttt
    38461 aatgcgggac agtaatttaa gttgtgtttt tgcaattgta agtgtgatat ttcgattcag
    38521 ttcgaattac ttcacatggg aataaaataa aaagaagcgg cctagtgtca gttgtgaata
    38581 cactgaacat tggtcgcttt atttagtagt acgatatgta gtttagctat taattttttt
    38641 caggtcatcc ttaatgctgt ctatctcaga catggcactt ttaacccaat ctccttgagc
    38701 cgcgccttta aaattagctt taaaagcctc gcaatgatgc gccatttgtt caattaatac
    38761 tttttcttca ccttttaatc cattttcaat atctttatat ttatgcttat gttcaggtgc
    38821 aacaactgaa cgaatgtttt ctttttgagc attcatttta gatatgagat taagtgtttc
    38881 tactgtagta cttatatctg gcattcttaa ggtcatatct ggttctatta gagtcattta
    38941 atctcctcca aattatcagt cacttagttt atctaactgc ttttcataag acttttttaa
    39001 gtcttcttta tattcttcta atttccattc ttgctttcta attatctcat tgatttcttg
    39061 ctcatacgct tctatatttc tcgtcatttt cctttgtgca tcttcactat aattcatttc
    39121 gtgtttcaaa tattgaaaaa catcataact acgttcattt tcttttcgag ttctatgcct
    39181 aagttcattt aaattttgca gttcattctc tatacgtttt ttctcagatt catatgattc
    39241 ttctaattca tttttattat taatttgatt aaacatcata gtaatccacc tatttgtgat
    39301 gcaagcactt gatctttatc aacgatttca ttaatactga gtctaactct cataatataa
    39361 tttgatacag aagaccctat cgttgaaagt tgtctttctt ttgcagatat catttgttca
    39421 ggttctctta ccatactact ttcattcact tgaccttcat ctaaagcatt gagaactcca
    39481 tttacactca aatcttcacc tacagctttt ccagcttttt gagcgtcttc ccaatttttt
    39541 ccaaatttct ttttcttttc attatacatt tttttgattt gattaatttc atcgtctatc
    39601 agttgactga atgactgcgc aattgttaaa gctgttaaac tttctaaaac tttttgctgc
    39661 gaagaagata atcctcctcc atttgtagtc atcatatttg ctctaagtaa ttctatacta
    39721 gctaatttag agatagcatt atttgaatgc tttacaaatg acttattatt ttcttctgca
    39781 taaccttgta gttttttaag ttcttttttt atagcttttt gttcttcttc ggtcagaaag
    39841 ccttccattt catgaccttt accattttta aagactttat cattaccgaa aaaaacataa
    39901 tgcttatatt tcttcagatt ttctgtcaat gcatcatttt ctgcaacaaa cctagtaata
    39961 ttacctttat aatttttata taactctaac aatcgttttc catctgggga attaggattc
    40021 aaactagtta tagaaatagg agctggatta taaaccacaa tgttcggaat accattactc
    40081 attcctagaa ctaccgcatc tcttccacct agtgaatgtc cagtaataaa atcaatatca
    40141 tacttacttt ttattttatt tataaattct tgcgtattcg catatctata gttttgatca
    40201 gatgcaggag aatataatat tttaagatct gcatatccat ctttaactgt ttccaaagca
    40261 ttggtaaaat tttgtcttga aaattcacct tcttttaact ttttaaaggc ttcgccttgt
    40321 atattagtcc cagtcatccc aagagttact ttgcctgtat ctttatttaa aaaagcagaa
    40381 gttgttactc cattaagaga atcagtataa ctatcaacat attcaagatt agaagggaat
    40441 tttttcgctt cttttaattg atctatttgt ctacctattg ttttactttt aattccatat
    40501 aatttcgcat tttcaatttc ataacttatt ttagaaatag ctgtagtttc ctcagtatta
    40561 tatttaatag gtcctggatc tattttgtat ttatctttca tcttcatcca ccttcgccgg
    40621 ctggaaattt aatgaatcta ttactgacaa atcgcttttt tcaccttttg aaaagtcaat
    40681 ttccaacttt ttataaccca cagaacttcc tttcaaatta ccatcaatat gcatttttaa
    40741 taccggtgct ttatcagtag gaatattata acgacttctt aattgcttaa cattttcatc
    40801 tttattgctc attttaaatt ttgcgtcata acttggaaca ttctcattaa ttgagacatc
    40861 accattgttg taatttttca attctttaag gtttgcatat tgtccgaaaa atttaaagtt
    40921 ttctattctc ttttttagtt ttggatcttc taccttgtct aataagataa ttttattatt
    40981 tttcatttca actttatatt tttttctatc tgggaattta tccttttcat aaaatgtctt
    41041 aacaaaataa tgtccctttg ctgtccgtgt atttctatct aagtataaga ccataccttc
    41101 attacttaat tcgtctgctt tattactttt ggcaaaatct gtatatatcg tccacatacc
    41161 tttatcacct tttttaaact caccatctcg atatccttct ttgtcatata gatcctcgag
    41221 attcttaatt ggatacatat ctaacgtttt cgcaaaactc tttttgattt gttcttcttt
    41281 tgaatctgtt ttattcgttt gttcctcttt tttctgttca tctttcatat ttccacatcc
    41341 cccaataaaa atgattaaaa gtaatacact catgtacaaa gcaatttttt ttatatactc
    41401 catcatttca catctctctg ctttaatttt ataaacattt gaatatttca tataacttat
    41461 taaaatagtg
//
LOCUS       DAMZLE010000009        39548 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMZLE010000009 DAMZLE010000000
VERSION     DAMZLE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.009"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(709..2091)
                     /locus_tag="POB92_000474"
     CDS             complement(709..2091)
                     /locus_tag="POB92_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6577998.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
     gene            2195..3082
                     /gene="pdxS"
                     /locus_tag="POB92_000475"
     CDS             2195..3082
                     /gene="pdxS"
                     /locus_tag="POB92_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDI6577999.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            3086..3646
                     /gene="pdxT"
                     /locus_tag="POB92_000476"
     CDS             3086..3646
                     /gene="pdxT"
                     /locus_tag="POB92_000476"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDI6578000.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3852..5066)
                     /locus_tag="POB92_000477"
     CDS             complement(3852..5066)
                     /locus_tag="POB92_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6578001.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            5224..5685
                     /locus_tag="POB92_000478"
     CDS             5224..5685
                     /locus_tag="POB92_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDI6578002.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            5704..6270
                     /locus_tag="POB92_000479"
     CDS             5704..6270
                     /locus_tag="POB92_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDI6578003.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            6260..7267
                     /locus_tag="POB92_000480"
     CDS             6260..7267
                     /locus_tag="POB92_000480"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDI6578004.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            7281..9737
                     /locus_tag="POB92_000481"
     CDS             7281..9737
                     /locus_tag="POB92_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDI6578005.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            10221..11585
                     /gene="radA"
                     /locus_tag="POB92_000482"
     CDS             10221..11585
                     /gene="radA"
                     /locus_tag="POB92_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI6578006.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            11610..12683
                     /locus_tag="POB92_000483"
     CDS             11610..12683
                     /locus_tag="POB92_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDI6578007.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            13240..14694
                     /locus_tag="POB92_000484"
     CDS             13240..14694
                     /locus_tag="POB92_000484"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI6578008.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            15117..15764
                     /gene="cysE"
                     /locus_tag="POB92_000485"
     CDS             15117..15764
                     /gene="cysE"
                     /locus_tag="POB92_000485"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI6578009.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15748..17148
                     /locus_tag="POB92_000486"
     CDS             15748..17148
                     /locus_tag="POB92_000486"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI6578010.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGV
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDRGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            17141..17545
                     /locus_tag="POB92_000487"
     CDS             17141..17545
                     /locus_tag="POB92_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDI6578011.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            17553..18299
                     /gene="rlmB"
                     /locus_tag="POB92_000488"
     CDS             17553..18299
                     /gene="rlmB"
                     /locus_tag="POB92_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI6578012.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            18299..18823
                     /locus_tag="POB92_000489"
     CDS             18299..18823
                     /locus_tag="POB92_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDI6578013.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18904..19473
                     /locus_tag="POB92_000490"
     CDS             18904..19473
                     /locus_tag="POB92_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDI6578014.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSVFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            19588..19731
                     /gene="rpmG"
                     /locus_tag="POB92_000491"
     CDS             19588..19731
                     /gene="rpmG"
                     /locus_tag="POB92_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6578015.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            19787..19969
                     /gene="secE"
                     /locus_tag="POB92_000492"
     CDS             19787..19969
                     /gene="secE"
                     /locus_tag="POB92_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI6578016.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            19982..20530
                     /gene="nusG"
                     /locus_tag="POB92_000493"
     CDS             19982..20530
                     /gene="nusG"
                     /locus_tag="POB92_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI6578017.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            20711..21133
                     /gene="rplK"
                     /locus_tag="POB92_000494"
     CDS             20711..21133
                     /gene="rplK"
                     /locus_tag="POB92_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI6578018.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            21341..22033
                     /gene="rplA"
                     /locus_tag="POB92_000495"
     CDS             21341..22033
                     /gene="rplA"
                     /locus_tag="POB92_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI6578019.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    22121..22267
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            22305..22805
                     /locus_tag="POB92_000496"
     CDS             22305..22805
                     /locus_tag="POB92_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI6578020.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEESAE"
     gene            22848..23216
                     /gene="rplL"
                     /locus_tag="POB92_000497"
     CDS             22848..23216
                     /gene="rplL"
                     /locus_tag="POB92_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI6578021.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            23391..23999
                     /locus_tag="POB92_000498"
     CDS             23391..23999
                     /locus_tag="POB92_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6578022.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKINGIDNAIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            24214..27765
                     /gene="rpoB"
                     /locus_tag="POB92_000499"
     CDS             24214..27765
                     /gene="rpoB"
                     /locus_tag="POB92_000499"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI6578023.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            27929..31525
                     /gene="rpoC"
                     /locus_tag="POB92_000500"
     CDS             27929..31525
                     /gene="rpoC"
                     /locus_tag="POB92_000500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI6578024.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            31662..31916
                     /locus_tag="POB92_000501"
     CDS             31662..31916
                     /locus_tag="POB92_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDI6578025.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            32014..32427
                     /gene="rpsL"
                     /locus_tag="POB92_000502"
     CDS             32014..32427
                     /gene="rpsL"
                     /locus_tag="POB92_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI6578026.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            32493..32963
                     /gene="rpsG"
                     /locus_tag="POB92_000503"
     CDS             32493..32963
                     /gene="rpsG"
                     /locus_tag="POB92_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI6578027.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            33086..35167
                     /gene="fusA"
                     /locus_tag="POB92_000504"
     CDS             33086..35167
                     /gene="fusA"
                     /locus_tag="POB92_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI6578028.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            35384..36568
                     /gene="tuf"
                     /locus_tag="POB92_000505"
     CDS             35384..36568
                     /gene="tuf"
                     /locus_tag="POB92_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6578029.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(36850..38025)
                     /locus_tag="POB92_000506"
     CDS             complement(36850..38025)
                     /locus_tag="POB92_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6578030.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            38196..39383
                     /locus_tag="POB92_000507"
     CDS             38196..39383
                     /locus_tag="POB92_000507"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI6578031.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
BASE COUNT        14151 a         5856 c         7676 g        11865 t
ORIGIN      
        1 aaaatggatg cgatgagccg cattgagacc gcaaggtctc tttttttatg tctaaaacgt
       61 caaaataaaa agcaaacaca aagaaaaatg gcttggcgaa gtgaaaacgt ttgaatctga
      121 cgaaacgaga aaagaacgca acgagtttag tagagctaaa tgagtaagtg agagccgtag
      181 gagaggaaag aagcaagcga ttgtcacaag tcaagaaagg ttcttagcga cgatggtagc
      241 caacttacgt tccgctagag tagaacgttg ccaggcaaaa atggatgcga tgagccgcat
      301 tgagaccgtg aggtctcttt ttttatgtct aaaacgtcaa aataaaagtt aacacaaaga
      361 aaatggcttg acgaagtgaa aacgtttgaa tctgacgaaa cgagaaaaga gcgcaacgag
      421 tttagtagag ctaaatgagt aagcgagagc cgaagaaaat gataatttaa taatgtacac
      481 tttcgattgt ctaagtatgt ataactttaa ttttgtgttt atataaattt aaaatgatat
      541 catcgaaaac aaaatattgt ataaatagag aagagcagta agacggtatc taattgaaaa
      601 tgatcttact gctcttttat atactttatt gaaatacaaa atggaaatta attattatac
      661 aatagacaag ctattgcata agtaacacta acttttatca aagaagtgtt actttataat
      721 taatgatttt attagagcgt ctacatgcgg ttttaaagca tcatcgtcta taccgccaaa
      781 gcctaatata aatttagggg ttttcttata gtcttgatca tcatcaaaat tataaacttg
      841 taattttaac tttactttgt ttgctctatc aagacactct tgtaatgtta atccattttt
      901 tactgtaatt gtaaaatgca tacccgtttc agcaccttga atatcaagct gctctttgta
      961 aggtttcaat ctttttaaaa tataggttag ttttctacga taaattcgtc tcattttatt
     1021 taaatgcctt tcaaaaccac cggaagatat aaacgttgca ataaggtttt gcatatgaac
     1081 aggtacagtg ttgccttcaa tgtgattttg agaatgatat tttttcatta tagaataggg
     1141 taacaccata tatgcaactc gacagctagg aaaaatagac tttgaaaatg tactgatata
     1201 aatcactttt tctcctcttg aatatagacc ttgaattgct ggaatgggtt tgccgaaata
     1261 tctaaactcg gaatcataat catcttctat aataaatcgt tcttcttttt cttgagccca
     1321 ttgtattaat tgagttcgct tttttaagtc catcacatat ccagttggaa attgatggga
     1381 aggcgttata tatactatat ttttttgtga tttaataact tcatctacgt ttattccatt
     1441 atcttcaact tcaatttgtt catattcaac ttgtttttta tctaaaatat ttttgattgg
     1501 tggataacta ggtttttcga taataaatgt tgaagtataa agtaaatcga ctaattgatt
     1561 tactaattgt tcggtagatg agccaattat aatttgatta ggatcacaaa ttacaccacg
     1621 attagtaaat aaataaaatg ccagttgaaa tcttaaatgt aattctcctt gaaaatgtcc
     1681 tctacgtaat tgatttaaat gatttgtatc ataaagatct ttggaatact ttctgaaaag
     1741 ttctataggg aaatgtttcg tatctatttc atccaaatta aaagcataat cataagcttc
     1801 atcactcgct tttggtttat atgaatcatc atcaaaaaga gaggggatag gttgattgtt
     1861 taaaattgtt aaagattcaa tttcggacac aaaatatcca gagcgaggtc ttgaataaat
     1921 gtaaccttcg tctaatagaa gttgatatgc atgctctacg gttgtttggc tgatagataa
     1981 atgtttgctt aattgtcttt tagaataaaa tttatcgcct tctttaaatt gaccttcaat
     2041 tatttgtttt tttaattttt cataaagttg atggtataaa gtgtttttca attttataac
     2101 tgacctccta aatttatctt attttgtacc tttttaaata tcagtttata cattacaatg
     2161 tatttaatca acttgaaaag gggttttatg tataatgagt aaaattattg gatcagacag
     2221 agtcaaaaga ggtatggctg aaatgcaaaa aggcggcgtt attatggatg tcgttaatgc
     2281 tgagcaagca agaattgcag aagaagcagg cgcggtagca gttatggcat tagaacgagt
     2341 accttctgat attagagctg ctggtggcgt tgcacgtatg gcaaacccta aaattgtaga
     2401 agaagtaatg aatgctgttt ctattccagt catggctaaa gcacgtattg gtcatatcac
     2461 tgaagcaaga gtattagagg cgatgggtgt tgactatatt gatgaatcag aagtgttaac
     2521 accagcagat gaggaatatc acttaagaaa agatcaattt acagtaccat ttgtatgtgg
     2581 atgtcgtaat ttaggtgaag ctgcgcgtag aattggtgaa ggtgctgcta tgttacgtac
     2641 taaaggtgaa ccaggtacag gtaatattgt tgaagctgta agacatatga gacaagttaa
     2701 ttcagaagtt agtcgattga ctgtaatgaa tgatgatgag attatgactt ttgcgaaaga
     2761 tatcggtgcg ccttatgaaa ttttaaaaca aattaaagac aatggtcgtt taccggtagt
     2821 taactttgca gctggtggcg ttgcgactcc tcaagatgct gctttaatga tggaattagg
     2881 tgccgacggt gtattcgttg gatcaggtat ttttaaatca gaagatccag aaaaatttgc
     2941 taaagcaatt gttcaagcaa caacacatta ccaagactat gagctaattg gaagattagc
     3001 aagtgaactt ggcactgcta tgaaaggttt agatatcaat caattatcat tagaagaacg
     3061 tatgcaagag cgtggttggt aagatatgaa aataggtgta ttagcattac aaggtgcagt
     3121 acgtgaacat attagacata ttgaattaag tggtcatgaa ggtattgcag ttaaaaaagt
     3181 tgaacaatta gaagaaatcg agggcttaat attacctggt ggcgagtcta caacgttacg
     3241 tcgattaatg aatttatatg gatttaaaga ggctttacaa aattcaactt tacctatgtt
     3301 tggtacatgc gcaggattaa tagttctagc gcaagatata gttggtgaag aaggatacct
     3361 taacaagttg aatattactg tacaacgaaa ctcattcggt agacaagttg acagctttga
     3421 aacagaatta gatattaaag gtatcgctac agatattgaa ggtgtcttta taagagcgcc
     3481 acatattgaa aaagtaggcc aaggcgtaga tatcctatgt aaggttaatg agaaaattgt
     3541 agccgtccag caaggtaaat atttaggcgt atcattccat cctgaattaa cagatgacta
     3601 tagagtaact gattacttta ttaatcatat tgtaaaaaaa gcatagctta atgtatgcta
     3661 aatcaacgaa ttattgatat ttatagattt gttgagaaga caatatctcc ttcaaactta
     3721 gctttggagg agctattttt ttatgtcaaa attaaaaatg ataaaaaata aagatataca
     3781 taaaaaaacc cttcaaagag actgagaata gtcaaaattt tgaaggggtt aattcgatgt
     3841 tgatgtattt gttaaataaa gaatccagcg attgcagctg aaatgaaaga tactagtgtt
     3901 gcaccgaata atagtttcaa accaaagcgg gcaactgtat ctcctttttt gtcattaagt
     3961 gatttaatcg cacctgaaat aataccaata gagctaaagt tagcaaatga tactaagaat
     4021 acagatgtaa caccttttgc gtgttcagat aaatcactaa gtttaccaag tgcttgcatt
     4081 gctacaaatt cgttagataa tagttttgtc gccataactg aaccggcttg aactgcatct
     4141 tgccatggca caccgactaa gaatgcaaat ggtgcaaaga caaaaccaat taatgtttgg
     4201 aaatcccaag aaatagcgcc acctgaaact gtactaaaga tattgcttac aattccattt
     4261 aatagagcga taatagcaat gtatccgatt aacattgcgc ctacaatgac agctacttta
     4321 aatccatcta aaatatattc tcctagcatt tcgaagaatg attgttgtct ttcttcagtt
     4381 tcttcaacta ataatttgtc atcttcttca ttaactttat aagggttaat aattgaagcg
     4441 atgatgaaac caccaaataa gtttaagaca acagccgtta caacatattt aggttcaatt
     4501 aaggtaaagt atgcaccgat aattgaagca gaaacagtcg acattgctga agctgttaat
     4561 gtgtataaac gttgcttagg tatgtatggt aattgttttt taattgaaat aaatacttca
     4621 gattgtccca aaattgctgc agcaactgca ttgtatgatt ctaaacgtcc cataccatta
     4681 attttagaaa ttaagaatcc taaaacatta atgattaaag gtaaaatctt tgtgtattga
     4741 aggataccga taatcgctga aataaatacg ataggtaata atacactgaa gaagaatggt
     4801 ggttgcttag gatcgatata ttgaatacca ccgaatacaa agttaacacc atctgctgct
     4861 tttaataata agtagttaaa accgtttgaa ataccaccaa taaccttgat tcccattgta
     4921 gttttaagca agataaatgc aaagataagc tgaattgcaa gtaaaattcc tacatatttc
     4981 cagcgaatat ttttcctgtc tgagctaaat agaaacgcaa gtgctaaaaa gaagataatt
     5041 ccgataatcc caattagaat atgcatatat ttctcattcc tttagttttt tctacaatct
     5101 atcatacaat aaaatggaag ggctaacatc ataaattttt gaaaatataa aaacaaatta
     5161 attgaaaaag gtcaaaatag gtcatataat atagtcaaag aaggtcaaaa aggggtgata
     5221 tacatgcaca atatgtctga catcatagaa caatacatca aacgtttatt tgaagagtcg
     5281 aatgaagatg tcgttgaaat tcagagagcg aatatcgcac agcgttttga ttgtgtacca
     5341 tcacaattaa attatgtaat caaaacacga ttcactaatg aacatggtta tgaaatcgaa
     5401 agtaaacgtg gtggtggtgg ttacatccga atcactaaaa ttgaaaataa agatgcaaca
     5461 ggttatatta atcatttgct tcagctgatt ggaccttcta tttctcaaca acaagcttat
     5521 tatattattg atgggctttt agataaaatg ttaataaatg aacgtgaagc taaaatgatt
     5581 caagcagtta ttgatagaga aacgctatca atggatatgg tttctagaga tattattaga
     5641 gcaaatattt taaaacgttt gttaccagtt ataaattatt actaaatgaa atgaggtgtt
     5701 gaagtgcttt gtgaaaattg tcaacttaat gaagcggaat taaaagttaa agttacaagt
     5761 aaaaataaaa cagaagaaaa aatggtgtgt caaacttgtg ctgaggggca ccatccgtgg
     5821 aatcaagcta atgaacaacc tgaatatcaa gaacatcaag ataatttcga agaagcattt
     5881 gttgttaagc aaattttaca acatttagct acgaaacatg gcattaattt tcaagaagta
     5941 gcgtttaaag aagaaaaacg ttgcccatca tgtcatatga ctttgaaaga tattgcacat
     6001 gttggtaaat ttgggtgtgc taattgttat gcaacattta aagatgacat cattgatatc
     6061 gtccgcagag ttcaaggtgg acaatttgag cacgttggaa agacaccaca ttcttcacat
     6121 aaaaagatag ctttaaagcg aaaaatcgaa gaaaagaatg aatatttgaa aaaacttatt
     6181 gaaatccaag attttgagga agcagccatt gttagagatg aaattaaagc actaaaagct
     6241 gagagtgagg tgcaacatga tgacgcataa tattcatgat aatatcagcc aatggatgaa
     6301 aagtaatgaa gaaacaccaa ttgttatgtc ttctagaatt cggttagcgc gtaatttaga
     6361 aaatcatgtg catccactaa tgtatgctac tgaaaatgat ggatttagag ttataaatga
     6421 ggtacaagat gccttgccaa attttgaatt aatgcgtctt gatcaaatgg atcaacaaag
     6481 taaaatgaaa atggttgcaa agcatttgat tagtcctgaa ctaataaaac aaccagcagc
     6541 cgcagtatta gtgaatgatg atgaatcttt aagtgtcatg ataaatgaag aggaccatat
     6601 tcgtattcaa gctatgggaa ctgacacgac attacaggct ttatataatc aagcttcatc
     6661 aattgatgat gaattagatc gaagccttga tataagttat gatgaacaac ttggttattt
     6721 aactacatgt cctaccaata taggtactgg tatgagagca agcgtgatgc tacatttacc
     6781 aggtctatct attatgaaaa gaatgacacg gattgctcaa accattaatc gttttggata
     6841 tacaatcaga ggtatttacg gtgaaggttc gcaagtttat ggacatactt atcaagtatc
     6901 caaccaactt acacttggta aatctgagtt agaaatcata gaaacattaa cagaagttgt
     6961 taatcaaatc attcatgaag aaaaacaaat acgacaaaag ttagacactt ataatcaatt
     7021 agaaacacaa gaccgtgttt ttcgctcgct aggtatttta caaaactgta gaatgataac
     7081 tatggaagag gcttcttata gattaagcga agttaaactt ggtatagatt taaattacat
     7141 tgaattacaa aactttaaat ttaatgaatt gatggtagct atacagtcac catttttatt
     7201 agatgaagaa gatgacaaat ctgtaaaaga aaaacgagca gatatactaa gagaacatat
     7261 aaagtaggag gtcattattt atgttatttg gtagattaac tgagcgtgca cagcgcgtat
     7321 tagcacatgc acaagaagaa gcaattcgtt taaatcattc aaatatagga acagaacacc
     7381 tattattggg attaatgaaa gaacctgaag gaattgctgc aaaagtatta gaaagtttta
     7441 atatcactga agataaagta attgaagaag ttgaaaaatt aatcggacat ggtcaagatc
     7501 atgttggtac attgcattat acacctagag ctaaaaaagt tattgaatta tcgatggatg
     7561 aagctagaaa attacatcac aattttgttg gaacggaaca tattttatta ggcttgattc
     7621 gtgaaaatga aggtgttgca gcaagagttt ttgcaaatct agatttaaat attactaaag
     7681 cacgtgcaca agttgtgaaa gctttaggaa accctgaaat gagtaataaa aatgcacaag
     7741 ctagtaagtc aaataatact ccaactttag atagtttagc tcgtgactta acagtcattg
     7801 ccaaagacgg tacattagat cctgttatag gacgtgataa agaaattaca cgtgtaattg
     7861 aagtattaag tagacgtacg aaaaacaatc ctgtacttat tggagagcca ggtgttggta
     7921 aaactgctat tgctgaaggt ttagcgcaag ccatagtgaa taatgaggta ccagagacat
     7981 taaaagataa gcgtgttatg tctttagata tgggaacagt agttgcaggt actaaatatc
     8041 gtggtgaatt tgaagagcgt ctgaaaaagg ttatggaaga aatccaacaa gcaggtaatg
     8101 tcatcctatt tattgatgag ttgcatactt tagttggtgc tggtggtgct gaaggtgcta
     8161 tcgatgcttc gaatattttg aaaccggcat tagcacgtgg tgaattacaa tgtattggtg
     8221 ctactacatt agatgaatat cgcaaaaata ttgaaaaaga cgcggcttta gaacgtcgtt
     8281 tccaacctgt acaagttgat gaaccttcag tagtagatac agttgctatt ttaaaaggat
     8341 taagagatcg ttacgaagca caccatcgta ttaatatttc agacgaagct attgaagcag
     8401 ctgttaaatt aagtaacaga tacgtttcag atcgtttctt accagataaa gcaattgatt
     8461 taattgatga agcaagttct aaagtaagac ttaagagtca tacgacacct aataatttaa
     8521 aagaaattga acaagaaatt gaaaaagtta aaaatgaaaa agatgctgca gtacatgctc
     8581 aagagtttga aaatgctgct aacctgcgtg ataaacaaac aaaacttgaa aagcaatatg
     8641 aagaagctaa aaatgaatgg aagaatgcac aaaatggcat gtcaacttca ttgtcagaag
     8701 aagatattgc tgaagttatt gcaggatgga caggtatccc attaactaaa atcaatgaaa
     8761 cagaatctga aaaacttctt agtctagaag atacattaca tgagagagtt attgggcaaa
     8821 aagatgctgt taattcaatc agtaaagcgg ttagacgtgc ccgtgcaggg ttaaaagatc
     8881 ctaaacgacc aattggtagc tttatcttcc ttggaccaac tggtgttggt aaaactgaat
     8941 tagctagagc tttagctgaa tcaatgtttg gcgatgatga tgcgatgatc cgtgtagaca
     9001 tgagtgaatt tatggaaaaa cacgcagtga gccgattagt tggtgctcct ccaggatatg
     9061 ttggtcatga tgatggtgga caattaactg aaaaagttag acgtaaacca tattctgtaa
     9121 ttttatttga tgaaattgaa aaagctcatc cagatgtatt taatattcta ttacaagttt
     9181 tagatgatgg acatttgaca gatacaaaag gacgtacagt tgatttcaga aatacaatta
     9241 tcataatgac atcaaacgtt ggggcacaag aattacaaga tcaacgattt gctggattcg
     9301 gtggttcaag tgatggacaa gattatgaaa caattcgaaa aacgatgtta aaagaattaa
     9361 aaaattcatt ccgtccagaa tttttaaacc gtgtagatga tatcattgta ttccataaac
     9421 taacaaaaga agaattaaaa gaaattgtaa caatgatggt taataaatta acaaatcgat
     9481 tatctgaaca aaacataaat attattgtta ctgataaagc gaaagacaaa atcgcagaag
     9541 aaggatatga tccagaatat ggtgcaagac cattaattag agcgatacaa aaaactatcg
     9601 aagataattt aagtgaatta atattagatg gtaatcaaat tgaaggtaag aaagttacag
     9661 tagatcatga tggtaaagag tttaaatatg acattgctga acaaacttca gaaactaaaa
     9721 caccatcgca agcataatta taaaacagtc caaaacaaat taaagttttg ggctgttttt
     9781 ttagtagcat tgaactatag aaattcgtga aagtatccat caacgaaaca atctataaaa
     9841 caatcatcaa aggatagtta agaattatat gtaacaagtt aatgagccta cagcgatagc
     9901 ataaaggtat gaatttttat aaaggttttt tgtttgaaga tactagcaac ttacgtcaaa
     9961 ataaaatagg tctatataat attgtgataa atctagagaa aagagtttac taagaaatta
    10021 ttaagatttt atctttgaaa gataccgact atcaacatga tgtaagttta ttttataata
    10081 ttcataaaaa ataaatctgg taaaacagtt tgcgttagta gtcatgttaa aatacaaaca
    10141 aatgtaactc acatttaatt tgtcataatg ggaatgtgcg tttaaaatag atttgttcaa
    10201 aggaaagtgg aggtgcaatt ttggccaaga aaaaagtgat ttttgaatgt atggcttgtg
    10261 gttatcaatc tcctaaatgg atggggaaat gtcctaattg tggcgcttgg aatcaaatgg
    10321 aggaaattgt tgaaaaagca gccaatccta aacatggagt taaaaccaag gaattagcag
    10381 gtaaagtaca aaaattaaat agtattaaac atgaaacaac gccgagagtg ttaacagatt
    10441 cagcagaatt caaccgtgta ttaggtggag gtattgtgag cggatcgtta gtacttattg
    10501 gtggggatcc aggtattggt aagtcaacgt tacttttaca aatttgtgca tcgttatctc
    10561 aaaagaaaaa agtactatat attactggag aagaatcgct tagtcagact aaattacgtg
    10621 cagagcgatt agatgaagat tcaagtgaat tgcaagtatt agctgaaaca gatcttgaag
    10681 ttatttatca aacagtaaaa gaagaacaac ctgatttatt agtagtggat tcgattcaaa
    10741 caatatatca tcctgaaatc agctctgcgc caggttctgt ttcacaagtt cgtgaaagta
    10801 cacaaagttt aatgaatatt gctaaacaaa tgaacattgc aacttttata gtgggtcatg
    10861 taacgaaaga aggtcaaatt gctggcccaa gattgctaga acacatggtt gatactgtgc
    10921 tttattttga aggcgatgaa caccacgcat atcgaatttt gcgagctgtt aaaaaccgtt
    10981 ttggttcaac gaatgaaatg ggaatcttcg aaatgaagca aagtggatta aaaggtgtaa
    11041 ataatccatc tgaaatgttt ttagaagaac gttcaacaaa tgttccaggt tcaacaattg
    11101 ttgcaaccat ggagggaacc agaccacttt taatagaagt tcaagcgctg gtaactccaa
    11161 cgacttttaa caatccgaga cgaatggcaa cagggattga tcataatcga ttaagtttgt
    11221 tgatggctgt tttggaaaag aaagaaaatt atctattaca acaacaagat gcttatatca
    11281 aagtagctgg cggtgtaaag ttaacggagc cagcagttga tttaagtgta attgtagcaa
    11341 ctgcatctag ctttaaagat aaagctgtcg acggattaga ttgctatatt ggagaagttg
    11401 gtttaacggg tgaggtacgt cgtgtatctc ggatagaaca acgcgtgcaa gaggctgcaa
    11461 aactaggttt caaacgtgta attattccta aaaataatat aggcggatgg acatatcctg
    11521 aaggtataca agtaataggt gtaactactg tacatgaagc attgtcattt gctcttcatt
    11581 cataaaacat caagaaagga ggacattgtg tgaatatcgt taaactaatg gttattatta
    11641 tttacttaat tattgggagc gcattaggaa taattattat tcctgaaatt gcaaatgatc
    11701 ttggattaca aaactccagc tttttaaaaa atcactatgt agatggcatt atcggtagta
    11761 tttttatgtt cttaattttt ggtgtattta ttagacgagt tactaacgct ataaaaggtt
    11821 tagaacattt tattatgcgt agaagtgctg ttgaaatact attcgcaaca ataggtttaa
    11881 taatcggatt acttatttct gttatggtgt cgtttatatt agaatcaatt ggtaactcta
    11941 tttttaatca tttcattcct gtcataatta cgatattact atgttatttc ggtttccaat
    12001 ttggccttaa aaaacgagat gaaatgttaa tgtttttacc tgagaatata gcgcgttcca
    12061 tgtcacaaca tactaaaagt gctacgccaa aaattatcga cacaagcgca attattgatg
    12121 gtcgtatttt agaagtcatt cgttgcggtt ttatcgatgg caatatttta attccacaag
    12181 gtgttattaa tgaattacaa attgttgcag attcaaatga cagtgttaaa cgtgaaaagg
    12241 gtaaaagagg cttagatatt ttaaatgaat tgtatgattt agactatcct acaaaggtta
    12301 tacatccaac taaaacacat agtgatattg atacgatgtt attaaaacta gcaaaacaat
    12361 atcatgcaag tattataacg acagatttca acctaaataa agtttgtcat gtacatggta
    12421 ttaaagcatt aaatgttaat gatttatcag aagcaatcaa acctaatgta catcaaggtg
    12481 atcaactgca tattttactg acaaaaatgg gtaaggagcc tggtcaggca gtaggatatc
    12541 tagatgatgg tacgatggtg gttgttgata atgctaaaaa tcttattggc agtcatgtca
    12601 atttagaagt agtcagctta ttgcaaacat cttcaggaag aattgttttt gctaaaaaaa
    12661 tcgaagatac agtatcatta taaaaactgt agttgacgaa aacaaaatag atattaaaag
    12721 gtatatgaaa tggcgaagtt tgtaatttga agtataacta taaataaatg caaaacatat
    12781 cagttatcaa aaaatgcaaa tagtgctaat atatcattga aacaagttca ttaagtgtta
    12841 aattttagat attaataaag ttattcttat agcaatcaag acatacaaaa ttagaatcaa
    12901 ttttaacgta tttgaatagt taatttgaag tttaatttca cagtacataa tattcatttt
    12961 taagtcgttg aaatgtttat gtagtgcagt cttgattctt agggatagct tttttaaagt
    13021 gttgaaaaaa caactgcttt ctatttaaaa tttaagcata ttacccaggg aattatgtta
    13081 tgattaaaca atgaaattat tttaaggtca tgttaaaaag cattgtttcg ttttaacaat
    13141 aagacgagtt agacatttgt tatgacggtc gaagatagca aatgaacaat ttatatgaac
    13201 taacttgaaa atctgatatt taatttagga gtgaaaaaaa tgagcgatcg tataagagta
    13261 agatatgcac caagtccaac tggttatctt catattggta atgcaagaac agcattattc
    13321 aattacttgt atgctaaaca ttacaacgga gattttgtga ttcgaattga agatactgat
    13381 aaaaaacgta atttagaaga tggagaaaca tcacaatttg ataatcttaa atggttagga
    13441 ttagattggg atgagtctgt agataaagac aatggctacg gaccatatcg tcaatctgaa
    13501 cgtcaacata tctaccaacc attaatagat cagttactag cagaagataa agcatataaa
    13561 tgctatatga cagaagaaga attagaagct gaacgtgaag cacaaatcgc tcgtggtgaa
    13621 atgcctcgct atggtggaca acatgcgcat ttgactgaag aacaacgtca acaatttgaa
    13681 gcagaaggac gccaaccatc aattcgtttc cgagtacctc aaaaccaaac gtattcattt
    13741 gatgatatgg taaaaggaaa tatttcattt gattcaaatg gtattggtga ctgggttatc
    13801 gtaaaaaaag atggcattcc aacgtacaat tttgcagtag ctatagatga tcattacatg
    13861 caaatttcag atgtaattcg tggtgatgat catatttcaa acacgcctaa acaaattatg
    13921 atttatgaag catttggctg ggagccacct cgttttggtc atatgtcatt aattgttaat
    13981 gaagaacgta aaaagttaag taaacgtgat gggcaaattt tacaatttat tgagcaatat
    14041 cgtgacttag gttatttacc tgaagcgtta tttaatttta ttgcgttatt aggttggtct
    14101 cctgaaggtg aagaagaaat cttttctaaa gaagaattta tcaaaatctt tgatgaaaag
    14161 cgtttgtcaa aatcaccagc atttttcgat aagcaaaaat tagcatgggt taataaccaa
    14221 tatatgaaac aaaaagatac tgaaacagta ttccaattag cattacctca tttaattaaa
    14281 gcaaatttga ttcctgaggt gccgtcagaa gaggatttat cttggggacg caaattaatt
    14341 gcgctttatc aaaaagaaat gagttatgcc ggtgaaattg tacctttatc agaaatgttc
    14401 tttaaagaaa tgccagctct tggtgaagaa gaacaacaag tgattaatgg agagcaagta
    14461 ccagagttaa tgacgcactt attcagtaaa ttagaagcac ttgaaccatt tgaagcggct
    14521 gaaattaaaa agacaattaa agaagttcaa aaagaaacag gaataaaagg caagcaatta
    14581 tttatgccta ttcgtgttgc tgtaacaggc caaatgcatg gtcctgaatt accaaataca
    14641 attgaagtac ttggtaaaga aaaagtgcta aaccgtttaa aacaatataa gtaatgaaat
    14701 agacaagatc aaggtagtat atacttgaat tttataacgt aaccacatat gataaagttg
    14761 tattaacaaa ttacaacaat atgaataaag tattttatat aaaacgaagg attagtaatt
    14821 aaatttatac gatgcagaga gtgtacggtt gctgtgagta caacgtagaa attaatgaat
    14881 gcaccttcgt aaaatgaatt aaatatataa tgagagtgat gagcattaag ttgacttagt
    14941 ttccttgata atttggaagc gcccgcaata ttattaatgt tattcgctaa attcagagtg
    15001 gaaccgtgcg gaagcgcctc taacaataca atttgtatgt tagtggtgct tttttgatat
    15061 ttaatttcgc aggtacttca attatgtatt tacgtagttc aatcattgag gaggaaatga
    15121 tcttgttaaa aagaatgaga gacgatataa aaatggtatt tgagcaggat ccagcggcac
    15181 gttcaacatt agaagtcatt acaacgtatg caggtttaca tgcagtttgg agtcatttga
    15241 ttgcacataa gttatacaac caaaaaaaat atgttgcagc acgcgcgata tctcaaattt
    15301 caagattttt cacaggtata gaaatccatc caggtgctaa aattggaaag cgtctattta
    15361 tagatcatgg tatgggcgtt gtaataggag aaacatgtac aattggtgat aatgtgacaa
    15421 tctatcaagg cgtgacactt ggtgggacag ggaaagaaag agggaaaaga cacccagata
    15481 taggagacaa tgttttaata gcagccggtg cgaaagtttt aggaaatatt aaaataaatt
    15541 caaatgtaaa tattggtgca aattcagttg ttttacaatc agttccaagt tattcaacgg
    15601 ttgttggtat accaggacat attgttaagc aagatggtgt tcgagttgga aaaacatttg
    15661 atcatcgcca tctacctgat ccaatttatg aacaaattaa gcatttagaa cgacaacttg
    15721 aaaagactag gaatggagag attcaagatg attacattat ataatacgct tacacgtcaa
    15781 aaagaagtgt tcaagcctat agaaccaggg aaagtaaaaa tgtatgtatg tggtcctact
    15841 gtatataact acattcatat tggtaacgca agaccagcaa ttaattatga cgtagtgaga
    15901 cgttactttg aataccaagg atataatgta gaatatgtat caaattttac agacgtagat
    15961 gataaattaa ttaaacgttc tcaagaatta aatcagtctg ttcccgaaat tgcagaaaaa
    16021 tatatcgctg cttttcatga agatgttggt gtgttaaatg ttagaaaagc gacttcaaat
    16081 ccaagggtaa tggaccatat ggatgacatt attcaattta ttaaagattt ggtggatcga
    16141 ggttatgcat atgaaagtgg tggcgatgtt tactttagaa cacgtaaatt tgaaggttat
    16201 ggtaaattaa gtcatcaatc catagatgac ttaaaagtgg gtgctcgtat agatgcagga
    16261 gagcataaag aagatgcact tgattttaca ttgtggaaaa aagcgaagcc tggcgagatt
    16321 agttgggata gcccatttgg tgaaggtaga ccaggatggc atatagaatg ttctgtaatg
    16381 gcatttcatg agctaggacc tacaattgat atacatgcgg gtggttcaga tttacaattt
    16441 ccacatcatg aaaatgaaat agcacaatca gaagcacata atcatgcgcc atttgctaat
    16501 tattggatgc ataatggttt cattaatatt gataatgaaa aaatgagtaa atcactaggc
    16561 aactttattt tagttcacga tattattaaa gaagttgatc cagatgtact aagattcttt
    16621 atgattagcg tacattatag aagcccaatt aactataatc tagaattggt agaatcagca
    16681 cgtagtggac tagagcgtat tcgcaatagt tatcaattaa ttgaagagcg cgcacaaatt
    16741 gctactaata ttgaaaatca acagacatat attgatcaaa ttgatgcgat tttaaatcgt
    16801 tttgaaacag ttatgaatga tgattttaat acagctaatg caattacagc ttggtatgat
    16861 ttagcaaaac ttgcgaataa atatgtacta gagaacacaa catcaacaga agtaattgat
    16921 aaatttaaag cagtttatca aattttcagc gatgttttag gtgtaccgtt aaaatctaaa
    16981 aatgcagatg aattattgga tgaagatgtt gaaaaattaa tcgaagagcg taatgaagca
    17041 aggaaaaaca aagattttgc acgagcagat gaaattcgag acatgctgaa atcacaaaac
    17101 attatattag aagacacacc tcaaggggtt agatttaaac gtggataatc aacaagataa
    17161 tcacattaaa ttattgaatc cattgacctt agcatatatg ggagacgcag tcttagatca
    17221 atatgtacgt acctatatcg ttttaaagct taaaagtaag cctaataaac tacatcaaat
    17281 gtctaaaaaa tatgtatctg ccaaaagtca ggcgcaaacg ttagaatatt taatggagca
    17341 agaatggttt acagacgaag aaatggatat tttgaagcga gggcgtaacg cgaaaagtca
    17401 tactaaagct aaaaacactg atgttcaaac atatcgtaaa agttcagcga tagaagcagt
    17461 gataggtttt ctttatttag aaaaaagaga agaacgatta gaggcattat taaataaaat
    17521 aataacaata gtaaacgaaa ggtagtgacg atgtggaaga tacggttatt gttggtaggc
    17581 atgctgttag agaagcgatt attactgggc atccgataaa taagatattg attcaagaag
    17641 gtattaaaaa gcaacaaatt aatgaaattt taaaaaatgc aaaagatcaa aaaatcattg
    17701 ttcaaactgt accaaaatct aaattagatt ttttagcaaa tgcaccacat cagggtgttg
    17761 cagcgcttat tgcaccatat gaatatgctg acttcgatca atttttaaaa cagcaaaaag
    17821 aaaaagaagg tttatcgaca gtacttatat tagacggctt agaagacccg cataacttgg
    17881 gatcaatttt aagaacagcc gatgcaacgg gagttgatgg tgttattatt cctaaacgtc
    17941 gttcagttac actaacgcaa acagttgcaa aagcctcaac aggtgcaatt gaacatgtac
    18001 cagttattcg agtgacaaat ttagctaaaa ctatcgatga actaaaagat aatggctttt
    18061 gggtagctgg cactgaagct aataatgcaa cagattatag aaatctagaa gcggacatgt
    18121 cattggctat tgtaattggt agcgaaggac agggtatgag tcgcctagta agtgataaat
    18181 gcgattttta tattaagatt ccaatggttg gacatgtaaa cagtttgaat gcttcggttg
    18241 cagcaagttt aatgatgtac gaagtatttc gaaaaagaca tgatgttgga gaaatataat
    18301 gaaagaacgt tacttaatca ttgatggata caatatgata ggacaatcac caacgctaag
    18361 cgccattgca aaagagaatt tagaagaagc tagaatgcaa ttaatagatg caattgcaaa
    18421 ttataatgca gttatttcag atgaaattat ttgtgttttc gatgcttatg accaatcggg
    18481 tgttgaaaga gaatacatgt atcatggcgt taaaacgatt tttaccaagg aaaaagaaac
    18541 agctgatagt ttcatagaac gttatgttta tgaactttat gacaagcata ctaagcatat
    18601 tacagttgta acaagtgata tgagtgagca acatgctatc tttggatcag gtgcatatag
    18661 aatatcatct cgcgaaatgt ggagagattt aaaagaaaat gaaattgatg tgagtaaatc
    18721 attagatgat ataagtgaaa acaagccaag aactcgaatt ccgttatctt ctgaaatcct
    18781 tgcagaattt gaaaaaatac gaagaggaca tcataagaaa tgacatttcc gtcatcttga
    18841 aattttgaat gtaacaatat taaactaacc ttaaatttta gatagaaggg gttagtttaa
    18901 tatttgaaat acgatttgac aactcaagac agtacaatca aacgtaacaa tgcaataaat
    18961 gataaagact tcgaaaagtt agtaatggat ctacaaccat taattattcg acgcatcaaa
    19021 acatttggat ttaatcatta tgatttagaa gacttatatc aagaaatact tatacggatg
    19081 tataggtcgg tccaaacatt tgattttagt ggagagcagc ctttcacaaa ttatgttcaa
    19141 tgtttaatta cgtctgtaaa gtatgattat ttgagaaaat atttagctac aaataaaaga
    19201 atggataatt tgattaatga atatagagtt acgtatccat gtgcaataaa gcgttatgat
    19261 gttgaaaaca attatttgaa taaattagca attaaagagt tgattcgtca gtttaagtat
    19321 ttgagtgtat ttgaaaaaga tgtcatgtat ttaatgtgtg aacaatataa gccgagagaa
    19381 attgctcaac tgatgcatgt aaaagagaaa gtgatttata atgccataca acgatgtaaa
    19441 aataaaataa aacgttattt caaaatgatt tgaaaagcgc cttaggacgt gaattgaatt
    19501 agaacgtgtt acttactgat ggtttgacat ttgttataaa ttttatgtat agtatactgg
    19561 tattataatg aataaaggtg aattattgtg agaaaaatac ctttaaattg tgaagcttgt
    19621 ggcaatagaa attataatgt tcctaagcaa gaaggctcgg caacaagatt aaccttaaag
    19681 aaatattgtc caaaatgtaa cgcgcacaca attcataaag aatcgaaata aatacattcg
    19741 aaataatact ttgataatat gttcaaagga tttggaggtt gagcagatgg ctaaaaaaga
    19801 aagtttcttt aaaggcgtta agtctgaaat ggaaaaaaca agttggccga cgaaagaaga
    19861 gctatttaaa tatactgtaa ttgtagtttc tactgttata ttcttcttag tctttttcta
    19921 tgccttagat ttaggaatta cagcattgaa aaatttatta tttggttaga ggagtgaaga
    19981 catgtctgaa gaagttggcg caaagcgttg gtatgcagtg catacatatt ctggatatga
    20041 aaataaagtt aaaaagaatt tagaaaaaag agtagaatct atgaatatga ctgaacaaat
    20101 ctttagagta gtcataccgg aagaagaaga aactcaagta aaagatggca aagctaaaac
    20161 gactgttaaa aaaacattcc ctggatatgt tttagtggaa ttaatcatga cagatgaatc
    20221 atggtatgtg gtaagaaata caccaggtgt tactggtttt gtaggttctg caggtgcagg
    20281 gtctaagcca aatccattgt taccagaaga agttcgcttc atcttaaaac aaatgggtct
    20341 taaagaaaag actatcgatg ttgaactcga agttggcgag caagttcgta ttaaatcagg
    20401 tccatttgcg aatcaagttg gtgaagttca agaaattgaa acagataagt ttaagctaac
    20461 agtattagta gatatgtttg gccgagaaac accagtagaa gttgaattcg atcaaataga
    20521 aaagctttaa ttaacaatta aagttattaa actaaccaaa agataaaaaa gagtattgat
    20581 tttttaatta gaaaagtgtt aaaattatgt ggtcgcgctt ttagagcgcc catttcgtca
    20641 cgaaatgtta agagtgggag ggcaaaactg agccctgtga ccacatcacg atatcaagga
    20701 ggtgcacatc gtggctaaaa aagtagataa agttgttaaa ttacaaattc ctgcaggtaa
    20761 agcgaatcca gcaccaccag ttggtccagc attaggtcaa gcaggtgtga acatcatggg
    20821 attctgtaaa gagttcaatg cacgtactca agatcaagca ggtttaatta ttccggtaga
    20881 aatcagtgtt tatgaagatc gttcatttac atttattaca aaaactccac cggctccagt
    20941 attacttaaa aaagcagctg gtattgaaaa aggttcaggc gaaccaaaca aaactaaagt
    21001 tgctacagta actaaagatc aagtacgcga aattgctaac agcaaaatgc aagacttaaa
    21061 cgctgctgac gaagaagcag ctatgcgtat tatcgaaggt actgcacgta gtatgggtat
    21121 cgttgtagaa taattttacg aatattaaat ttgattacat gatttaaacg atgaagcaga
    21181 taacagagat aataatgatg aattataaat ataatctgaa tgactagatt aatgattgat
    21241 ttattcataa gattaattct tctgttgtct gctcttaact tgcatatagc aagtaatgtg
    21301 ggaggaaatt ccgctaaaac cactaaagga ggaactataa atggctaaaa aaggtaaaaa
    21361 gtatcaagaa gcagctagta aagttgaccg tactcagcac tacagtgttg aagaagcaat
    21421 taaattagct aaagaaacaa gcattgctaa ctttgacgct tctgttgaag ttgcattccg
    21481 tttaggaatt gatacacgta aaaatgacca acaaatccgt ggtgcagttg tattaccaaa
    21541 cggaactggt aaatcacaaa gtgtattagt attcgctaaa ggtgacaaaa ttgctgaagc
    21601 tgaagcagca ggtgctgact atgtaggtga agcagaatac gttcaaaaaa tccaacaagg
    21661 ttggttcgac ttcgatgtag tagttgctac accagacatg atgggtgaag ttggtaaatt
    21721 aggtcgtgta ttaggaccaa aaggtttaat gccaaaccct aaaactggaa ctgtaacaat
    21781 ggatgttaaa aaagctgttg aagaaatcaa agctggtaaa gtagaatatc gtgctgaaaa
    21841 agctggtatc gtacatgcat caattggtaa agtttcattt actgatgaac aattaattga
    21901 aaacttcaat actttacaag atgtattagc taaagctaaa ccatcatctg ctaaaggtac
    21961 atacttcaaa tctgttgctg taactacaac aatgggtcct ggagttaaaa ttgatactgc
    22021 aagtttcaaa taataaataa tataaacaat tacaggctga aagaaatatc tttcagtctg
    22081 taaaaatata ttgacaataa gtaatttcca agttatatta cttattgtga ttattttacc
    22141 taagacagta ggagttattt ataacttaaa atttatcctg ccgaggctaa aattgacttg
    22201 aacgtgatga tctatgatct ttcaagcact ttttgccgtg ggtagaaagt gcttttttta
    22261 ttaattttaa aaaaagcacc aaaaatttaa atggaggtgt ctgaatgtct gctatcattg
    22321 aagctaaaaa acaactagtt gatgaaattg ctgaggtact atcaaattca gtttcaacag
    22381 taatcgttga ctaccgtgga ttaacagtag ctgaagttac tgacttacgt tcacaattac
    22441 gtgaagctgg tgttgagtat aaagtataca aaaacactat ggtacgtcgt gcagctgaaa
    22501 aagctggtat cgaaggctta gatgaattct taacaggtcc tactgctatt gcaacttcaa
    22561 gtgaagatgc tgtagctgca gcgaaagtaa tttctggatt tgctaaagat catgaagcat
    22621 tagaaattaa atcaggcgtt atggaaggca atgttattac agcagaagaa gttaaaactg
    22681 ttggttcatt accttcacac gatggtcttg tatctatgct tttatcagta ttacaagctc
    22741 ctgtacgcaa cttcgcttat gcggttaaag ctattggaga acaaaaagaa gaaagcgctg
    22801 aataattttt agcgtaaaaa aattaaaaat aatggaggaa ttataaaatg gctaatcatg
    22861 aacaaatcat tgaagcgatt aaagaaatgt cagtattaga attaaacgac ttagtaaaag
    22921 caattgaaga agaatttggt gtaactgcag ctgctccagt agcagtagca ggtgcagctg
    22981 gtggcgctga cgctgcagca gaaaaaactg aatttgacgt tgagttaact tcagctggtt
    23041 catctaaaat caaagttgtt aaagctgtta aagaagcaac tggtttagga ttaaaagatg
    23101 ctaaagaatt agtagacgga gctcctaaag taatcaaaga agctttacct aaagaagaag
    23161 ctgaaaaact taaagaacaa ttagaagaag ttggagctac tgtagaatta aaataattca
    23221 agtatcttaa acttaataat caaagtttta tagcaagtat tgctataata taatgattct
    23281 ttgagaagtt aaaaccccgt tattttgata acggggtttt attcatttaa agactgagtg
    23341 aaatgttata attataatga cgagttacaa agtgaagatg aggtggaata atgagtcatt
    23401 attacgatga agatccaagt gtaattagca atgaacaacg tattcaatat caattaaacc
    23461 atcataaaat tgatttaata actgataatg gagtgttttc gaaagataaa gtagattatg
    23521 gttcagatgt tcttgttcaa acttttttaa aagcgcatcc acctggtcca agtaagcgaa
    23581 ttgccgatgt tggttgtggt tacggaccaa ttggtttgat gattgctaaa gtatcaccac
    23641 atcattcaat tacaatgcta gatgttaatc acagagcgct agccttagtt gaaaaaaaca
    23701 aaaaaataaa tggtattgat aatgcgatcg taaaggaaag tgatgctttg tctgctgtgg
    23761 aagacaaaag ttttgatttt attttaacca atccaccaat aagagcaggg aaagaaaccg
    23821 tgcatcgtat attcgagcaa gcattacata gattagactc gaacggtgaa ctattcgttg
    23881 taattcagaa gaagcaaggt atgccatctg caaagaaaag aatgaatgaa ctttttggaa
    23941 atgtagaagt ggtaaataaa gataaaggat attacattct gagaagtata aaagcttgaa
    24001 atgaaatgga tattctgtta tagttatata atgtaaaaat ttatgttcaa taagtgtgta
    24061 cttttacgtt aaatagataa gttaattaag aataaatata gaatcgaaaa tggtgtcatc
    24121 attagtgttg ccgttttctt tttgtctttt tattaatatg cttatggtat ttagctaaaa
    24181 gcggatcaca taatttttga ggggtgaatc tgtttggcag gtcaagttgt ccaatatgga
    24241 agacatcgta aacgtagaaa ctacgcgaga atttcagaag tattagaatt accaaactta
    24301 atagaaattc aaactaaatc ttacgagtgg ttcctaagag aaggtttaat cgaaatgttt
    24361 agagacattt ctccaattga agattttact ggtaatttgt cattagagtt tgtggattac
    24421 cgtttaggag aaccaaaata tgatttagaa gaatctaaaa accgtgacgc tacttatgct
    24481 gcacctcttc gtgtaaaagt gcgtctaatc attaaagaaa caggagaagt taaagaacaa
    24541 gaagtcttta tgggtgattt cccattaatg actgatacag gtacgttcgt tatcaatggt
    24601 gcagaacgtg taatcgtatc tcaattagtt cgttcaccat ccgtttattt caatgaaaaa
    24661 atcgacaaaa atggtcgtga aaactatgat gcaacaatta ttccaaaccg tggtgcatgg
    24721 ttagaatatg aaacagatgc taaagatgtt gtatacgtac gtattgatag aacacgtaaa
    24781 ctaccattaa cagtattgtt acgtgcatta ggtttctcaa gcgaccaaga aattgttgac
    24841 cttttaggtg acaatgaata tttacgtaat actttagaga aagacggcac tgaaaacact
    24901 gaacaagcgt tattagaaat ctatgaacgt ttacgtccag gtgaaccacc aactgttgaa
    24961 aatgctaaaa gtctattgta ttcacgtttc tttgatccaa aacgctatga cttagcaagc
    25021 gtgggtcgtt ataaaacaaa caaaaaatta catttaaaac atcgtttatt taatcaaaaa
    25081 ttagctgagc caattgtaaa tactgaaact ggtgaaattg tagttgaaga aggtacagtg
    25141 cttgatcgtc gtaaaatcga cgaaatcatg gatgtacttg aatcaaatgc aaacagcgaa
    25201 gtgtttgaat tgcatggtag cgttatagac gagccagtag aaattcaatc aattaaagta
    25261 tatgttccta acgatgatga aggtcgtacg acaactgtaa ttggtaatgc tttccctgac
    25321 tcagaagtta aatgcattac accagcagat atcattgctt caatgagtta cttctttaac
    25381 ttattaagcg gtattggata tacagatgat attgaccatt taggtaaccg tcgtttacgt
    25441 tctgtaggtg aattactaca aaaccaattc cgtatcggtt tatcaagaat ggaaagagtt
    25501 gtacgtgaaa gaatgtcaat tcaagatact gagtctatca cacctcaaca attaattaat
    25561 attcgacctg ttattgcatc tattaaagaa ttctttggta gctctcaatt atcacaattc
    25621 atggaccaag caaatccatt agctgagtta acgcataaac gtcgtctatc agcattagga
    25681 cctggtggtt taacacgtga acgtgctcaa atggaagtac gtgacgttca ctactctcac
    25741 tatggccgta tgtgtccaat tgaaacacct gagggaccaa acattggatt gattaactca
    25801 ttatcaagtt atgcacgtgt aaatgaattc ggctttattg aaacaccata tcgtaaagtt
    25861 gatttagata cacatgctat cactgatcaa attgactatt taacagctga cgaagaagat
    25921 agctatgttg tagcacaagc aaactctaaa ttagatgaaa atggtcgttt catggatgat
    25981 gaagttgtat gtcgtttccg tggtaacaac acagttatgg ctaaagaaaa aatggattat
    26041 atggatgtat cgccgaagca agttgtttca gcagcgacag catgtattcc attcttagaa
    26101 aatgatgact caaaccgtgc attgatgggt gcgaacatgc aacgtcaagc agtgcctttg
    26161 atgaatccag aagcaccatt tgttggtaca ggtatggaac acgttgcagc acgtgattct
    26221 ggtgcagcta ttacagctaa gcacagaggt cgtgttgaac atgttgaatc taatgaaatt
    26281 cttgtacgtc gtctagttga agagaacggc gttgagcatg aaggtgaatt agatcgctat
    26341 ccattagcta aatttaaacg ttcaaactca ggtacatgtt acaaccaacg tccaatcgtt
    26401 gcagttggag atgttgttga gtataacgag attttagcag atggaccatc tatggaatta
    26461 ggagaaatgg cattaggtag aaacgtagta gttggtttca tgacttggga cggttacaac
    26521 tatgaggatg ccgttatcat gagtgaaaga cttgtgaaag atgacgtgta tacttctatt
    26581 catattgaag agtatgaatc agaagcacgt gatactaagt taggacctga agaaatcaca
    26641 agagatattc ctaatgtttc tgaaagtgca cttaagaact tagacgatcg tggtatcgtt
    26701 tatattggtg cagaagtaaa agatggagat attttagttg gtaaagtaac gcctaaaggt
    26761 gtaactgagt taactgccga agaaagattg ttacatgcaa tctttggtga aaaagcacgt
    26821 gaagttagag atacttcatt acgtgtacct cacggcgctg gcggtatcgt tcttgatgta
    26881 aaagtattca atcgcgaaga aggcgacgat acattatcac ctggtgtaaa ccaattagta
    26941 cgtgtataca tcgttcaaaa acgtaaaatt catgttggtg ataagatgtg tggtcgacat
    27001 ggtaacaaag gtgtcatttc taagattgtt cctgaagaag atatgcctta cttaccagac
    27061 ggacgtccga tcgatatcat gttaaaccct cttggtgtac catctcgtat gaacatcgga
    27121 caagtattag agctacactt aggtatggct gctaaaaatc ttggtattca cgttgcatca
    27181 ccagtatttg acggcgcaaa cgatgacgat gtatggtcaa caattgaaga agctggtatg
    27241 gctcgtgatg gtaaaactgt actttatgat ggacgtacag gtgaaccatt cgataaccgt
    27301 atttcagtag gtgtaatgta catgttgaaa cttgcgcaca tggttgatga taaattacat
    27361 gcgcgttcaa caggaccata ttcacttgtt acacaacaac cacttggagg taaagcgcaa
    27421 ttcggtggac aacgtttcgg tgagatggag gtatgggcac ttgaagcata tggtgctgca
    27481 tacacattac aagaaatctt aacttacaaa tccgatgata cagtaggacg tgtgaaaaca
    27541 tacgaggcta ttgttaaagg tgaaaacatc tctagaccaa gtgttccaga atcattccga
    27601 gtattgatga aagaattaca aagtttaggt ttagatgtaa aagttatgga tgagcaagat
    27661 aatgaaatcg aaatgacaga cgttgatgac gatgatgttg tagaacgtaa agtagattta
    27721 caacaaaatg atgctcctga aacacaaaaa gaagttactg attaatacgc aatttacaaa
    27781 acaggcaaaa agatactaag ctgaatttta ttgatgattc agtttagtac tttaagccat
    27841 tttaaataaa tgcaaatcaa tcaaatagca cagctaatct aaattgaagg aggtaggctc
    27901 cttgattgat gtaaataatt tccattatat gaaaatagga ttggcttcac ctgaaaaaat
    27961 ccgttcttgg tcttttggtg aagttaaaaa acctgaaaca atcaactacc gtacattaaa
    28021 acctgaaaaa gatggtctat tctgtgaaag gattttcgga cctacaaaag actgggaatg
    28081 tagttgtggt aaatacaaac gtgttcgcta caaaggcatg gtctgtgaca gatgtggagt
    28141 tgaagtaact aaatctaaag tacgtcgtga aagaatgggt cacattgaac ttgctgctcc
    28201 agtttctcac atttggtatt tcaaaggtat accaagccgt atgggattat tacttgacat
    28261 gtcaccaaga gcattagaag aagttattta ctttgcttct tatgttgttg tagatccagg
    28321 tccaactggt ttagaaaaga aaactttatt atctgaagct gaattcagag attattatga
    28381 taaataccca ggtcaattcg ttgcaaaaat gggtgcagaa ggtattaaag atttacttga
    28441 agagattgat cttgacgaag aacttaaatt gttacgcgat gagttggaat cagctactgg
    28501 tcaaagactt actcgtgcaa ttaaacgttt agaagttgtt gaatcattcc gtaattcagg
    28561 taacaaacct tcatggatga ttttagatgt acttccaatc atcccaccag aaattcgtcc
    28621 aatggttcaa ttagatggtg gacgatttgc aacaagtgac ttaaacgatt tataccgtcg
    28681 tgtaattaat cgaaataatc gtttgaaacg tttattagat ttaggtgcac ctggtatcat
    28741 cgttcaaaac gaaaaacgta tgttacaaga agccgttgac gctttaattg ataatggtcg
    28801 tcgtggtcgt ccagttactg gcccaggtaa ccgtccatta aaatctttat ctcatatgtt
    28861 aaaaggtaaa caaggtcgtt tccgtcaaaa cttacttggt aaacgtgttg actattcagg
    28921 acgttcagtt attgcagtag gtccaagctt gaaaatgtac caatgtggtt tgccaaaaga
    28981 aatggcactt gaactattta aaccattcgt aatgaaagaa ttagttcaac gtgaaattgc
    29041 aactaacatt aaaaatgcga agagtaaaat cgaacgcatg gatgatgaag tttgggacgt
    29101 attggaagaa gtaattagag aacatcctgt attacttaac cgtgcaccaa cacttcatag
    29161 acttggtatt caagcatttg aaccaacttt agttgaaggt cgtgcgattc gtctacatcc
    29221 acttgtaaca acagcttata acgctgactt tgacggtgac caaatggcgg ttcacgttcc
    29281 tttatcaaaa gaggcacaag ctgaagcaag aatgttgatg ttagcagcac aaaacatctt
    29341 gaaccctaaa gatggtaaac ctgtagttac accatcacaa gatatggtac ttggtaacta
    29401 ttaccttact ttagaaagaa aagatgcagt aaatacaggc gcaatcttta ataatacaaa
    29461 tgaagtatta aaagcatatg caaatggctt tgtacattta cacactagaa ttggtgtaca
    29521 tgcaagttcg ttcaataatc caacatttac tgaagaacaa aacaaaaaga ttcttgctac
    29581 gtcagtaggt aaaattatat tcaatgaaat cattccagat tcatttgctt atattaatga
    29641 acctacgcaa gaaaacttag aaagaaagac accaaacaga tatttcatcg atcctacaac
    29701 tttaggtgaa ggtggattaa aagaatactt tgaaaatgaa gaattaattg aacctttcaa
    29761 caaaaaattc ttaggtaata ttattgcaga agtattcaac agatttagca tcactgatac
    29821 atcaatgatg ttagaccgta tgaaagactt aggattcaaa ttctcatcta aagctggtat
    29881 tacagtaggt gttgctgata tcgtagtatt acctgataag caacaaatac ttgatgagca
    29941 tgaaaaatta gtcgacagaa ttacaaaaca attcaaccgt ggtttaatca ctgaagaaga
    30001 aagatataat gcagttgttg aaatttggac agatgcaaaa gatcaaattc aaggtgaatt
    30061 gatgcaatca cttgataaaa ctaacccaat cttcatgatg agtgattcag gtgcccgtgg
    30121 taacgcatct aactttacac agttagcagg tatgcgtgga ttgatggccg caccatctgg
    30181 taagattatc gaattaccaa tcacatcttc attccgtgaa ggtttaacag tacttgaata
    30241 cttcatctca actcacggtg cacgtaaagg tcttgccgat acagcactta aaacagctga
    30301 ctcaggatat cttactcgtc gtcttgttga cgtggcacaa gatgttattg ttcgtgaaga
    30361 agactgtggt actgatagag gtttattagt ttctgatatt aaagaaggta cagaaatgat
    30421 tgaaccattt atcgaacgta ttgaaggtcg ttattctaaa gaaacaattc gtcatcctga
    30481 aactgatgaa ataatcattc gtcctgatga attaattaca cctgaaattg ctaagaaaat
    30541 tacagatgct ggtattgaac aaatgtatat tcgctcagca tttacttgta acgcacgaca
    30601 tggtgtttgt gaaaaatgtt acggtaaaaa ccttgctact ggtgaaaaag ttgaagttgg
    30661 tgaagcagtt ggtacaattg cagcccaatc tatcggtgaa ccaggtacac agcttacaat
    30721 gcgtacattc catacaggtg gggtagcagg tagcgatatc acacaaggtc ttcctcgtat
    30781 tcaagagatt ttcgaagcac gtaaccctaa aggtcaagcg gtaattacgg aaatcgaagg
    30841 tgtcgtagaa gatattaaat tagcaaaaga tagacaacaa gaaattgttg ttaaaggtgc
    30901 taatgaaaca agatcatacc ttgcttcagg tacttcaaga attattgtag aaatcggtca
    30961 accagttcaa cgtggtgaag tattaactga aggttctatt gaacctaaga attacttatc
    31021 tgttgctgga ttaaacgcga ctgaaagcta cttattaaaa gaagtacaaa aagtttaccg
    31081 tatgcaaggt gtagaaatcg acgataaaca cgttgaggtt atggttcgac aaatgttacg
    31141 taaagttaga attatcgaag caggtgatac gaagttatta ccaggttcat tagttgatat
    31201 tcataacttt acagatgcaa atagagaagc atttaaacac cgtaagcgtc ctgcaacagc
    31261 taaaccagta ttacttggta ttactaaagc atcacttgaa acagaaagtt tcttatctgc
    31321 agcatcattc caagaaacaa caagagttct tacagatgca gcaattaaag gtaagcgtga
    31381 tgacttatta ggtcttaaag aaaacgtaat tattggtaag ttaattccag ctggtactgg
    31441 tatgagacgt tatagcgacg taaaatacga aaaaacagct aaaccagttg cagaagttga
    31501 atctcaaact gaagtaacgg aataacaagt atataacaga ggctaatgct ttagcctctt
    31561 gttattttta tgtaaattat ttgatttaat gttgacgaat tctcttgttc aatgttaata
    31621 tattaaaggt tgatgcaagc agaactttgg aggataaatt attgtctaag gaaaaagttg
    31681 cacgctttaa caaacaacat tttgtagttg gtcttaaaga aacgcttaaa gcgttaaaga
    31741 aagatcaagt tacatctttg attattgctg aagacgttga agtatattta atgactcgcg
    31801 tgttaagcca aatcaatcag aaaaatatac ctgtatcttt tttcaaaagc aaacatgctt
    31861 tgggtaaaca tgtaggtatt aacgtcaatg cgacaatagt agcattgatt aaatgagaat
    31921 tagtaagtgt tttacttact aaattttatt taacctaaaa atgaaccacc tggatgtgtg
    31981 ggattaaaaa gtgaagagag gaggacatat cacatgccaa ctattaacca attagtacgt
    32041 aaaccaagac aaagcaaaat caaaaaatca gattctccag ctttaaataa aggtttcaac
    32101 agtaaaaaga aaaaatttac tgacttaaac tcaccacaaa aacgtggtgt atgtactcgt
    32161 gtaggtacaa tgacacctaa aaaacctaac tcagcgttac gtaaatatgc acgtgtgcgt
    32221 ttatcaaaca acatcgaaat taacgcatac atccctggta tcggccataa cttacaagaa
    32281 cacagtgttg tacttgtacg tggtggacgt gtaaaagact taccaggtgt gcgttaccat
    32341 attgtacgtg gagcacttga tacttcaggt gttgacggac gtagacaagg tcgttcatta
    32401 tacggaacta agaaacctaa aaactaagaa tttagttttt aattaaatct taaacttaaa
    32461 atatttaata taaggaaggg aggatttaca ttatgcctcg taaaggatca gtacctaaaa
    32521 gagacgtatt accagatcca attcataact ctaagttagt aactaaatta attaacaaaa
    32581 ttatgttaga tggtaaacgt ggaacagcac aaagaattct ttattcagca ttcgacctag
    32641 ttgaacaacg cagtggtcgt gatgcattag aagtattcga agaagcaatc aacaacatta
    32701 tgccagtatt agaagttaaa gctcgtcgtg taggtggttc taactatcaa gtaccagtag
    32761 aagttcgtcc agagcgtcgt actactttag gtttacgttg gttagttaac tatgcacgtc
    32821 ttcgtggtga aaaaacgatg gaagatcgtt tagctaacga aattttagat gcagcaaata
    32881 atacaggtgg tgccgttaag aaacgtgagg acactcacaa aatggctgaa gcaaacaaag
    32941 catttgctca ctaccgttgg taagataaaa gcttttaccc tgagtgtgtt ctatattaat
    33001 gaattttcat taagcgttca tgcttagggc atcgccatat ctatcgtatt tattcagtaa
    33061 tataaactgg aaggagaaaa aatacatggc tagagaattt tcattagaaa aaactcgtaa
    33121 tatcggtatc atggctcaca ttgatgctgg taaaacgact acgactgaac gtattcttta
    33181 ttacactggc cgtatccaca aaattggtga aacacacgaa ggtgcttcac aaatggactg
    33241 gatggagcaa gaacaagacc gtggtattac tatcacatct gctgcaacaa cagcagcttg
    33301 ggaaggtcac cgtgtaaaca ttatcgatac acctggacac gtagacttca ctgtagaagt
    33361 tgaacgttca ttacgtgtac ttgacggagc agttacagta cttgatgcac aatcaggtgt
    33421 tgaacctcaa actgaaacag tttggcgtca ggctacaact tatggtgttc cacgtatcgt
    33481 atttgtaaac aaaatggaca aattaggtgc taacttcgaa tactctgtaa gtacattaca
    33541 tgatcgttta caagctaacg ctgctccaat ccaattacca attggtgcgg aagacgaatt
    33601 cgaagcaatc attgacttag ttgaaatgaa atgtttcaaa tatacaaatg atttaggtac
    33661 tgaaattgaa gaaattgaaa ttcctgaaga ccacttagat agagctgaag aagctcgtgc
    33721 tagcttaatc gaagcagttg cagaaactag cgacgaatta atggaaaaat atcttggtga
    33781 cgaagaaatt tcagtttctg aattaaaaga agctatccgc caagctacta ctaacgtaga
    33841 attctaccca gtactttgtg gtacagcttt caaaaacaaa ggtgttcaat taatgcttga
    33901 cgctgtaatt gattacttac cttcaccact agacgttaaa ccaattattg gtcaccgtgc
    33961 tagcaaccct gaagaagaag taatcgcgaa agcagacgat tcagctgaat tcgctgcatt
    34021 agcgttcaaa gttatgactg acccttatgt tggtaaattg acattcttcc gtgtgtattc
    34081 aggtacaatg acatctggtt catacgttaa gaactctact aaaggtaaac gtgaacgtgt
    34141 aggtcgttta ttacaaatgc acgctaactc acgtcaagaa atcgatactg tatactctgg
    34201 agatatcgct gctgcggtag gtcttaaaga tacaggtact ggtgatactt tatgtggtga
    34261 gaaaaatgac attatcttgg aatcaatgga attccctgag ccagttattc acttatcagt
    34321 agagccaaaa tctaaagctg accaagataa aatgactcaa gctttagtta aattacaaga
    34381 agaagaccca acattccatg cacacactga cgaagaaact ggacaagtta tcatcggtgg
    34441 tatgggtgag cttcacttag acatcttagt agaccgtatg aagaaagaat tcaacgttga
    34501 atgtaacgta ggtgctccaa tggtttcata tcgtgaaaca ttcaaatcat ctgcacaagt
    34561 tcaaggtaaa ttctctcgtc aatctggtgg tcgtggtcaa tacggtgatg ttcacattga
    34621 attcacacca aacgaaacag gcgcaggttt cgaattcgaa aacgctatcg ttggtggtgt
    34681 agttcctcgt gaatacattc catcagtaga agctggtctt aaagatgcta tggaaaatgg
    34741 tgtcttagca ggttatcctt taattgatgt taaagctaaa ttatatgatg gttcatacca
    34801 tgatgtcgat tcatctgaaa tggccttcaa aattgctgca tcattagcac ttaaagaagc
    34861 tgctaaaaaa tgtgatcctg taatcttaga accaatgatg aaagtaacta ttgaaatgcc
    34921 tgaagagtac atgggtgata tcatgggtga cgtaacatct cgtcgtggac gtgttgatgg
    34981 tatggaacct cgtggtaatg cacaagttgt taatgcttat gtaccacttt cagaaatgtt
    35041 cggttatgca acatcattac gttcaaacac tcaaggtcgc ggtacttaca ctatgtactt
    35101 cgatcactat gctgaagttc caaaatcaat cgctgaagat attatcaaga aaaataaagg
    35161 tgaataatat aacttgtttt gactagctag cctaggttaa aatacaaggt gagcttaaat
    35221 gtaagctatc atctttatag tttgattttt tggggtgaat gcattataaa agaattgtaa
    35281 aattcttttt gcatcgctat aaataatttc tcatgatggt gagaaactat catgagagat
    35341 aaatttaaat attattttta attagaatag gagagatttt ataatggcaa aagaaaaatt
    35401 cgatcgttct aaagaacatg ccaatatcgg tactatcggt cacgttgacc atggtaaaac
    35461 aacattaaca gcagcaatcg ctactgtatt agcaaaaaat ggtgactcag ttgcacaatc
    35521 atatgacatg attgacaacg ctccagaaga aaaagaacgt ggtatcacaa tcaatacttc
    35581 tcacattgag taccaaactg acaaacgtca ctacgctcac gttgactgcc caggacacgc
    35641 tgactacgtt aaaaacatga tcactggtgc tgctcaaatg gacggcggta tcttagtagt
    35701 atctgctgct gacggtccaa tgccacaaac tcgtgaacac attcttttat cacgtaacgt
    35761 tggtgtacca gcattagtag tattcttaaa caaagttgac atggttgacg atgaagaatt
    35821 attagaatta gtagaaatgg aagttcgtga cttattaagc gaatatgact tcccaggtga
    35881 cgatgtacct gtaatcgctg gttcagcatt aaaagcttta gaaggcgatg ctcaatacga
    35941 agaaaaaatc ttagaattaa tggaagctgt agatacttac attccaactc cagaacgtga
    36001 ttctgacaaa ccattcatga tgccagttga ggacgtattc tcaatcactg gtcgtggtac
    36061 tgttgctaca ggccgtgttg aacgtggtca aatcaaagtt ggtgaagaag ttgaaatcat
    36121 cggtttacat gacacatcta aaacaactgt tacaggtgtt gaaatgttcc gtaaattatt
    36181 agactacgct gaagctggtg acaacattgg tgcattatta cgtggtgttg ctcgtgaaga
    36241 cgtacaacgt ggtcaagtat tagctgctcc tggttcaatt acaccacata ctgaattcaa
    36301 agcagaagta tacgtattat caaaagacga aggtggacgt cacactccat tcttctcaaa
    36361 ctatcgtcca caattctatt tccgtactac tgacgtaact ggtgttgttc acttaccaga
    36421 aggtactgaa atggtaatgc ctggtgataa cgttgaaatg acagtagaat taatcgctcc
    36481 aatcgcgatt gaagacggta ctcgtttctc aatccgtgaa ggtggacgta ctgtaggatc
    36541 aggcgttgtt actgaaatca ttaaataatt tctaatttct tagattttat ataaaaagaa
    36601 gatccctcaa tcgaggggtc tttttttaat gtgtaaattt tgtaatggct attcgattta
    36661 gaagaacaat aattgatgaa agactgacta ataaaactta taactgataa tactgtttaa
    36721 ataaaattgt tgagtcttgg acattgtaaa atgctccctt caaagttttc attttttcaa
    36781 tgtctacttt gaagggagca tttcattagt ttatgtctca gattcatatc tttcaattaa
    36841 tttaaatgct taatttgttt taaatacttg ctctaattca atgattttta aaaatacagc
    36901 tacagcgtat tttaatgatt tttcatcaat atcaaatttg ggattatggt gtggcgctgt
    36961 aataccttta ctttcattac cacaaccagt cagaaagaat gcacctggtc gtactttcaa
    37021 ataatgtgaa aaatcttctc caatcatcat taaatctgat tcattaaagc gtacatgtaa
    37081 gtcatttgtt gcttctttaa taacttgata tgctttctcg ttattatgga caggcaaata
    37141 ccctttaata taattcaaat catagttaat atcatttgct attgctaaac cttgtaaaag
    37201 cttatccatt ttgtccatta catgattctg tatatctgaa tcgaaagttc taactgtacc
    37261 tttacaaaat gcttgatcag gaataacgct atctgtggtg cctgcttgaa tcattccaaa
    37321 tgaaagtaca gcttgtttaa ctggatcgat cgtacgtgaa attatttttt gtgcacttaa
    37381 aatgaactct gccatgatta ctattgggtc aatggtttca tgaggtttgg caccatgacc
    37441 gccacgacct ttaattgtga cactaaattc atctggagag gccatgattg cccccgcacg
    37501 tgaatgaata gttccagtag gataaccact ccataaatgt gtaccgtaaa ttctatctac
    37561 attttccaga catccagcat ctatcatttc ttgagaacca cctggcatga tttcttcacc
    37621 gtactggaat attaatacaa cattaccttc taataaatgt ttatgttcat ctaaaatctc
    37681 tgctacagta agtaaaattg ctgtatgacc atcatgccca cacgcatgca tacatcctgg
    37741 atttttagac ttataaggca catcgtttaa ttcctcgaca ggtaacgcat caaagtcagc
    37801 tcttaatgca atggtaggtc ctgtgcccaa gcctttaaat gtggctttga taccattgcg
    37861 gccgatagga gtttcaatat cacaagataa ctggcttaat tggttaacaa tataatcatg
    37921 tgtttgaaat tcttcaaaag ataactcagg atattggtgt aaataacgtc tgagttgaat
    37981 tgttttattt tctttattat ttgctagttg gaaccaatct aacaccctta tcactacttt
    38041 ctaaaataat gtttatagta taacatttta tgaaattatc gtactaaatg attgctttga
    38101 gatattttat ctatgaatga taaggctttc aagttatgta gaattactgt atgataaagg
    38161 tattaccaaa caatacttaa gggggattat atactgtggt tcaatcatta catgagtttt
    38221 tagaggaaaa tataaattat ctaaaagaaa atggtttgta taatgaaata gatacaattg
    38281 aaggtgcaaa cggaccagaa atcaaaatca atgggaaatc atacattaac ttatcttcaa
    38341 ataattattt aggactagca acaaatgaag atttgaaatc agctgcaaaa gcagctattg
    38401 atacacatgg agtaggtgca ggcgctgttc gtacaatcaa tggtacatta gatttacacg
    38461 acgaattaga agaaacacta gcaaaattta aaggaacaga agctgcaata gcttatcaat
    38521 caggatttaa ttgtaatatg gctgctattt cagctgtcat gaataaaaat gatgctattt
    38581 tatcagatga gcttaatcat gcatcaatta ttgatggatg tcgcttatct aaagctaaaa
    38641 ttattcgagt taaccattca gacatggatg atttacgtgc gaaagcaaaa gaagcagttg
    38701 aatcaggtca atacaataaa gtgatgtata tcactgatgg cgtttttagt atggatggtg
    38761 atgtggctaa attacctgaa attgtagaaa ttgcagaaga atttggttta ttaacttatg
    38821 tagacgacgc tcatggttca ggtgttatgg gtaaaggcgc tggtacggtt aaacattttg
    38881 gtttacaaga taaaatcgat ttccaaatag gtacgctttc taaagcaatt ggtgtcgttg
    38941 gcggttatgt agcaggtaca aaagagttaa tagattggtt aaaagcacaa tcacgaccat
    39001 tcttattctc tacatcatta gcacctgggg ataccaaagc aataactgaa gcagttaaaa
    39061 agttaatgga ttcaactgaa ttacatgata aattatggga caatgcacaa tatttaaaaa
    39121 atggattgtc aaaattagga tatgatacag gtgagtcaga aactccaatt acaccagtaa
    39181 ttattggtga tgaaaaaaca actcaagaat ttagtaagcg tttaaaagac gaaggtgtct
    39241 atgtgaaatc tatcgttttc ccaacagtac caagaggtac aggacgtgta agaaatatgc
    39301 ctacagctgc acatacaaaa gacatgttag atgaagcaat tgcggcttat gaaaaagtag
    39361 gaaaagaaat gaagttgatt taatatttat ttattcccac ggcaaatatt gtcgtgggct
    39421 ttttttaaat gtttagttta ttaacagtaa gttcttataa caatgtttag tgctcccaaa
    39481 aattaaagtt ttaattttaa aagcatcttg taaaatttag ttgtattttt ttcaaagaaa
    39541 ttcatttt
//
LOCUS       DAMZLE010000010        36716 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMZLE010000010 DAMZLE010000000
VERSION     DAMZLE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.010"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(74..181)
                     /locus_tag="POB92_000508"
     CDS             complement(74..181)
                     /locus_tag="POB92_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578032.1"
                     /translation="MVLLTFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(218..541)
                     /gene="isdG"
                     /locus_tag="POB92_000509"
     CDS             complement(218..541)
                     /gene="isdG"
                     /locus_tag="POB92_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDI6578033.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(560..1294)
                     /gene="srtB"
                     /locus_tag="POB92_000510"
     CDS             complement(560..1294)
                     /gene="srtB"
                     /locus_tag="POB92_000510"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDI6578034.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1356..2321)
                     /gene="isdF"
                     /locus_tag="POB92_000511"
     CDS             complement(1356..2321)
                     /gene="isdF"
                     /locus_tag="POB92_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDI6578035.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLITKFTTG
                     NNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2334..3212)
                     /gene="isdE"
                     /locus_tag="POB92_000512"
     CDS             complement(2334..3212)
                     /gene="isdE"
                     /locus_tag="POB92_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDI6578036.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3209..4285)
                     /gene="isdD"
                     /locus_tag="POB92_000513"
     CDS             complement(3209..4285)
                     /gene="isdD"
                     /locus_tag="POB92_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDI6578037.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYILFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4285..4968)
                     /gene="isdC"
                     /locus_tag="POB92_000514"
     CDS             complement(4285..4968)
                     /gene="isdC"
                     /locus_tag="POB92_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDI6578038.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            5177..6229
                     /gene="isdA"
                     /locus_tag="POB92_000515"
     CDS             5177..6229
                     /gene="isdA"
                     /locus_tag="POB92_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDI6578039.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            6432..8369
                     /gene="isdB"
                     /locus_tag="POB92_000516"
     CDS             6432..8369
                     /gene="isdB"
                     /locus_tag="POB92_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDI6578040.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQK
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(8523..8696)
                     /gene="rpmF"
                     /locus_tag="POB92_000517"
     CDS             complement(8523..8696)
                     /gene="rpmF"
                     /locus_tag="POB92_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI6578041.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(8776..9333)
                     /locus_tag="POB92_000518"
     CDS             complement(8776..9333)
                     /locus_tag="POB92_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDI6578042.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            9460..10599
                     /locus_tag="POB92_000519"
     CDS             9460..10599
                     /locus_tag="POB92_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDI6578043.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(10661..11143)
                     /gene="coaD"
                     /locus_tag="POB92_000520"
     CDS             complement(10661..11143)
                     /gene="coaD"
                     /locus_tag="POB92_000520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI6578044.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(11145..11687)
                     /gene="rsmD"
                     /locus_tag="POB92_000521"
     CDS             complement(11145..11687)
                     /gene="rsmD"
                     /locus_tag="POB92_000521"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDI6578045.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            11757..12146
                     /locus_tag="POB92_000522"
     CDS             11757..12146
                     /locus_tag="POB92_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578046.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(12149..12403)
                     /locus_tag="POB92_000523"
     CDS             complement(12149..12403)
                     /locus_tag="POB92_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDI6578047.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            12642..13568
                     /locus_tag="POB92_000524"
     CDS             12642..13568
                     /locus_tag="POB92_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6578048.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVSTKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(13631..14065)
                     /locus_tag="POB92_000525"
     CDS             complement(13631..14065)
                     /locus_tag="POB92_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDI6578049.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(14081..15118)
                     /locus_tag="POB92_000526"
     CDS             complement(14081..15118)
                     /locus_tag="POB92_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDI6578050.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(15445..15906)
                     /locus_tag="POB92_000527"
     CDS             complement(15445..15906)
                     /locus_tag="POB92_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDI6578051.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(15931..16842)
                     /locus_tag="POB92_000528"
     CDS             complement(15931..16842)
                     /locus_tag="POB92_000528"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDI6578052.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            17282..18193
                     /locus_tag="POB92_000529"
     CDS             17282..18193
                     /locus_tag="POB92_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDI6578053.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLLIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(18333..21785)
                     /locus_tag="POB92_000530"
     CDS             complement(18333..21785)
                     /locus_tag="POB92_000530"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDI6578054.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEYGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(22339..23565)
                     /gene="ftsW"
                     /locus_tag="POB92_000531"
     CDS             complement(22339..23565)
                     /gene="ftsW"
                     /locus_tag="POB92_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDI6578055.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(23879..24154)
                     /locus_tag="POB92_000532"
     CDS             complement(23879..24154)
                     /locus_tag="POB92_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDI6578056.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            24295..24777
                     /locus_tag="POB92_000533"
     CDS             24295..24777
                     /locus_tag="POB92_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578057.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            24779..24946
                     /locus_tag="POB92_000534"
     CDS             24779..24946
                     /locus_tag="POB92_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDI6578058.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(25086..26933)
                     /gene="typA"
                     /locus_tag="POB92_000535"
     CDS             complement(25086..26933)
                     /gene="typA"
                     /locus_tag="POB92_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDI6578059.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            27035..27226
                     /locus_tag="POB92_000536"
     CDS             27035..27226
                     /locus_tag="POB92_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDI6578060.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(27380..28207)
                     /locus_tag="POB92_000537"
     CDS             complement(27380..28207)
                     /locus_tag="POB92_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDI6578061.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            28364..28978
                     /locus_tag="POB92_000538"
     CDS             28364..28978
                     /locus_tag="POB92_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDI6578062.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERNFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            29162..30514
                     /locus_tag="POB92_000539"
     CDS             29162..30514
                     /locus_tag="POB92_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI6578063.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVYIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(30596..31015)
                     /locus_tag="POB92_000540"
     CDS             complement(30596..31015)
                     /locus_tag="POB92_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6578064.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(31298..32317)
                     /locus_tag="POB92_000541"
     CDS             complement(31298..32317)
                     /locus_tag="POB92_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6578065.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISINIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(32391..33464)
                     /locus_tag="POB92_000542"
     CDS             complement(32391..33464)
                     /locus_tag="POB92_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6578066.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(33464..34273)
                     /locus_tag="POB92_000543"
     CDS             complement(33464..34273)
                     /locus_tag="POB92_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6578067.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(34279..35076)
                     /locus_tag="POB92_000544"
     CDS             complement(34279..35076)
                     /locus_tag="POB92_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6578068.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(35069..36163)
                     /locus_tag="POB92_000545"
     CDS             complement(35069..36163)
                     /locus_tag="POB92_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578069.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(36176..36715)
                     /locus_tag="POB92_000546"
     CDS             complement(36176..36715)
                     /locus_tag="POB92_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6578070.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
BASE COUNT        12378 a         6492 c         5101 g        12745 t
ORIGIN      
        1 atatcattat taattctttt cttcttcaaa ataaatcaaa atgatataat tgatgattat
       61 tttcaaagca cattcaaatc aaactatgtt ttagcaattt gttgttagca tgtttgtgtt
      121 cattaaaaaa acgaccatca tcggtatcat gtatggtcgt tacaaaagtt aacaatacca
      181 attgtcataa caagtactgc aacctcttta aattcaatta tttcatgtaa ctatagccta
      241 tatcatatgt aattactttg ttattgataa tcgggctact ttcatcttca tttttacttc
      301 taacatgttt atgtgctgct ttaaagacat cagattttaa ccaatccgta aaagcttgct
      361 ttgatttcca aactgttaaa attttcactt catcaaaatc ttcctgttct aaagtttgtg
      421 taacaaacat gccatcaaag ccttctaatg tttcaatccc atgtctcgtg taaaatcgtt
      481 ctataatatc ttttgctgtt ccttttgtta acgtcagcct attttctgcc ataaatttca
      541 taattatcct cttttctgtt taacttacct taattatttt tgcgacaaca acaattcttt
      601 tcgttgtttc actatatgca tcttcgcacg ttgataaagt cattattcta tcttttaccg
      661 ttacattaac atctgaatta attacagatt tacgttttgt ttcatctaaa aattgttgat
      721 aatcttgatc attttcaaaa tctgtacgta tgtaattatc tttagtagta gttttatatg
      781 cactaaatac ttgcaattga tatttaccat atttattgtc aaattcaatt atcttgtgtt
      841 tttcataaaa cgattgcttt aaataatctt ctaacacatc aaacatcgta ttatcaccga
      901 catggtgccc gtataaaata gtattatgat ttaaattctt caattcattt ctaaaatcca
      961 taaaaatact acctttacgt cgatgttctc gctcaaaatc taaatttaaa taatcgtgat
     1021 ttgtcttacc ttgtagtact ggataattta atgatgttcc tgataatttt atccatccaa
     1081 caatgtcttt atttattttt tcaagtgatt caaattgtgg tctcacatgt gcttgatgtt
     1141 tgctcatcag catttgaaat ttttgttgta atttctcata atttgcgcgt tcttgcttgt
     1201 cttcaatata tgtttgaaca attttgtaac caaaaatgat aataattaca accaataaaa
     1261 tttgtacaat agttaaaaat cgcttcattc tcataaaaat cctcttttat taacgacgtt
     1321 tcttcagtca tcactaaacc agttgttgta ccgttttaga ttcgatttcg ttgactttga
     1381 caaattaagt aaattagcat tggaccaccg acaatcatta aaatagcatt ggctggaatt
     1441 tctagaggag gctgtatcac tcgtcctaat aaatcagcca ctaacaatag ccatgcacca
     1501 ataactgtag aaaacggaat aagtactctg taattgcccc caactagctt tctaaccaca
     1561 tgtggcacaa taatacctaa aaaggctagt tgtccaacaa tcgcaacagt tgcacttgct
     1621 aaaaatactg ctaataaacc tgttaaccat ctgtaacgat caatattaaa accgatactt
     1681 cgcgcttgta tgtcgtctaa atttagtaaa ttcaatttag gggacaatag taatgttaat
     1741 attaatccca ataatgctga tactgctaat atgtatacgt cgctccatat tttcattgtt
     1801 aagccttgag gaattttcat taaagggttt tgagttaaaa tttctaaaac accatttaat
     1861 aatacgaata acgcaacacc tactaatatc atacttacag cattgaatct aaatttagaa
     1921 tgcaacaata taattattaa aaatggtatt aaacctccaa taaaacttaa taatggtaag
     1981 taaaagtaca attgtggaat aaacaacata caaagtgctc tcattataag tgcacctgag
     2041 gaaacgccaa tgatattcgc ctctgccaaa ggattttgta gtgctgcttg taataatgct
     2101 ccagaaactg ctaacattgc gccaaccatc aatgcaatta atatacgtgg caatcgcaaa
     2161 tcaatgattg aatccactgc ttcattgtta ccagttgtaa attttgtaat taggtcatta
     2221 aatgacaatt taattgtacc ggttacaaac gaaatataag cagttgcgat taaaatgact
     2281 aacaaacata aaaatagtag tttcttttta ttttttatca tcatatcaac tcactatttt
     2341 ttatccttat aaaataaatc atataattga gtcattgcct tatcagcatc aacatttgct
     2401 gtaataccga atggcacttc ctctaagtca taaacatgat tatttttcac agctttaaaa
     2461 tgtttccaaa tatcattctg tttaaattct ttttgaaaca ttttcttaac ttcttcaggc
     2521 attccgtgtg gtaatcttaa aataatatct ggattgatat tcaacaaatt ttcagtatta
     2581 gacgaaatat attgacgatc tttcacttta ataacatttt ctccacctgc tattttaact
     2641 aaatcaccaa tatatgattt atcagttgct actaaatagc tacccggtac acccattaat
     2701 attaatactt tgggatgttt cttttgttta gctgctttat tttcaatttt ttgctttaca
     2761 gaatttaaat ggtcatttaa ttcttttgct tgtgctttac gattaaattg atcacctaat
     2821 tgtgtaatcg acttttgcat cccttttaaa ctatcaaaat cataaaagta gcctttcata
     2881 tttaattgtt tgtaaaatgg ttgcatttca tctttaatcg ttgacacact caaaacatgt
     2941 gttggtttta attttttaac agcttcaaca ttcggctcca ttggttgacc aatttccggt
     3001 acatctttat aacgattagg caatgtctta tatgacgtgg gtttgccgac aattggtaaa
     3061 tccaatttgt ccaatgtcat tgtcaatgca acagttgttg gtacgattct gaattcgccg
     3121 gatttagttg attcttgaga actggaagat tgacagctgg ttaagataac gacgcttatc
     3181 actaaaatgg ttaaatactt tatgattctc aaacttggtt acctctcttt ctgctgtata
     3241 aatgtgccga aataagtaaa atagttgcta acacaatgcc agctgcaaat aaaatatatt
     3301 gctttttatg atttggttca tcaagttctt taatatcact agataattga ctactattct
     3361 tgctatcatc atctctatta aaagctggta acgctttttc tttttcaact acttttttct
     3421 tttccttttt tgatttttca tctttatgtt tcggttgctc tttgtgatta ttttcaacct
     3481 tttttactgt tggagggcta ttttcaactt ttaatgtacc tgatgcttta ggtcgctcat
     3541 tttcaatctt tttattactg tcttgttgca ctttatcttt ttgtacagga atatcattta
     3601 atcctttttc agtaaaaagg ctagtacgtc tttccttagt atctgatttt tgactatctt
     3661 ttttgtcatc aatatctttt ttaagctgat cttgattatt ttcttgattt tgatgttttg
     3721 aaggcacttc ttttgcatca tcatgttgta cttttttctc tttaaaatga atagaataca
     3781 ccatttcttt gttatctaga tttttctttt cagccaacgt tttaacatgc atattgattt
     3841 tatccatatg ctgttcaatt aaaattctta ctattgtgtc atccttttca tcttttataa
     3901 caaaaggaaa ttcttcattt ttgtcatttt gaaaaacaag ttgttcttta tctatttgac
     3961 cttttagctt tacttgcaag taaattttac cagtcatatc tgaaatcaat tgcgcctttt
     4021 cgtcaactag ttgttgttcc tcagttacat ttttattttc ttgattaaaa attagtaact
     4081 ctaacgcttt attctctttt atcttttcat caaaaattga tgtagttaat tctgttttat
     4141 cttttgcttc aggttgatta aatttggctg atggatattt tatctcatca gccaacacgc
     4201 taccaatcat tgttgtatat gtaagtatac ctaagctaat aatagctata atagattttg
     4261 tagcaatttg tttaacattt ctcattattc cacattgcct ttagattttt ttctaacaaa
     4321 caatgtgatt gcgattaata atgctaagga tgcaactggt attgcatata tatatgcagg
     4381 tgtaccagca tttgtttgtg gattttctac tttgtcttta ttcgaactat tagattcact
     4441 ttgaccagta gtcgttccat cagatccttt gtcactacct gaagcagaat ttttatcatc
     4501 tttacctggt gcattagcac ctgctacatc agttggtcca ttaaatttat atgtaatgtt
     4561 gtaatgatgg tcatatttga atggctttcc atttactttt tcatcgatat aaacgtcaat
     4621 ttttccatct attttaccgt tcaacttact tacttcaaat tcagaagtgc gttcatcttt
     4681 ggcagtgttt ttactaataa tattttcttt atgtccttcg atactcattc cagtaatcca
     4741 atgactgtgg ttgacagtta tttgaacata caatttacca tttttcttaa tgtactttgc
     4801 cggtttatta aaatagtcat tagcaattga cgtgtcattg gtattgtatt tgtaaacctc
     4861 ataattcaaa gtaccgctat ctgcggcatt tgcagaatta ctgaatgtcg cgatgatgat
     4921 aattaacgct aaaatcgttg tattaaaaac ttttaaaata tttttcaaaa cataatcctc
     4981 ctttttatga ttgcttttaa gtctttagta aaatcataaa taataatgat tatcattgtc
     5041 aatatttatt ttataatcaa tttattattg ttatacgaaa atagatgtgc tagtataatt
     5101 gataaccatt atcaattgca atggttaatc atctcatata acaacacata atttgtatcc
     5161 ttaggaggaa aacaacatga caaaacatta tttaaacagt aagtatcaat cagaacaacg
     5221 ttcatcagct atgaaaaaga ttacaatggg tacagcatct atcattttag gttcccttgt
     5281 atacataggc gcagacagcc aacaagtcaa tgcggcaaca gaagctacga acgcaactaa
     5341 taatcaaagc acacaagttt ctcaagcaac atcacaacca attaatttcc aagtgcaaaa
     5401 agatggctct tcagagaagt cacacatgga tgactatatg caacaccctg gtaaagtaat
     5461 taaacaaaat aataaatatt atttccaaac cgtgttaaac aatgcatcat tctggaaaga
     5521 atacaaattt tacaatgcaa acaatcaaga attagcaaca actgttgtta acgataataa
     5581 aaaagcggat actagaacaa tcaatgttgc agttgaacct ggatataaga gcttaactac
     5641 taaagtacat attgtcgtgc cacaaattaa ttacaatcat agatatacta cgcatttgga
     5701 atttgaaaaa gcaattccta cattagctga cgcagcaaaa ccaaacaatg ttaaaccggt
     5761 tcaaccaaaa ccagctcaac ctaaaacacc tactgagcaa actaaaccag ttcaacctaa
     5821 agttgaaaaa gttaaaccta ctgtaactac aacaagcaaa gttgaagaca atcactctac
     5881 taaagttgta agtactgaca caacaaaaga tcaaactaaa acacaaactg ctcatacagt
     5941 taaaacagca caaactgctc aagaacaaaa taaagttcaa acacctgtta aagatgttgc
     6001 aacagcgaaa tctgaaagca acaatcaagc tgtaagtgat aataaatcac aacaaactaa
     6061 caaagttaca aaacataacg aaacgcctaa acaagcatct aaagctaaag aattaccaaa
     6121 aactggttta acttcagttg ataactttat tagcacagtt gccttcgcaa cacttgccct
     6181 tttaggttca ttatctttat tacttttcaa aagaaaagaa tctaaataaa tcatcgtcac
     6241 actcataact taatatattt tttattttaa attttattta acctatgtca tagatatttc
     6301 ataatctata acataggtta tttttttata aaataacgtt gcaattaact accatttcaa
     6361 tgtacaatac aagtaatcaa ttgataatga ttatcagttg ataatataca attaggagtt
     6421 gtttttacaa catgaacaaa cagcaaaaag aatttaaatc attttattca attagaaagt
     6481 catcactagg cgttgcatct gtagcaatta gtacactttt attattaatg tcaaatggcg
     6541 aagcacaagc agcagctgaa gaaacaggtg gtacaaatac agaagcacaa ccaaaaactg
     6601 aagcagttgc aagtccaaca acaacatctg aaaaagctcc agaaactaaa ccagtagcta
     6661 atgctgtctc agtatctaat aaagaagttg aggctcctac ttctgaaaca aaagaagcta
     6721 aagaagttaa agaagttaaa gcccctaagg aaacaaaaga agttaaacca gcagcaaaag
     6781 ccactaacaa tacatatcct attttgaatc aggaacttag agaagcgatt aaaaaccctg
     6841 caataaaaga caaagatcat agcgcaccaa actctcgtcc aattgatttt gaaatgaaaa
     6901 agaaagatgg aactcaacag ttttatcatt atgcaagttc tgttaaacct gctagagtta
     6961 ttttcactga ttcaaaacca gaaattgaat taggattaca atcaggtcaa ttttggagaa
     7021 aatttgaagt ttatgaaggt gacaaaaagt tgccaattaa attagtatca tacgatactg
     7081 ttaaagatta tgcttacatt cgcttctctg tatcaaacgg aacaaaagct gttaaaattg
     7141 ttagttcaac acacttcaat aacaaagaag aaaaatacga ttacacatta atggaattcg
     7201 cacaaccaat ttataacagt gcagataaat tcaaaactga agaagattat aaagctgaaa
     7261 aattattagc gccatataaa aaagcgaaaa cactagaaag acaagtttat gaattaaata
     7321 aaattcaaga taaacttcct gaaaaattaa aggctgagta caagaagaaa ttagaggata
     7381 caaagaaagc tttagatgag caagtgaaat cagctattac tgaattccaa aaagtacaac
     7441 caacaaatga aaaaatgact gatttacaag atacaaaata tgttgtttat gaaagtgttg
     7501 agaataacga atctatgatg gatacttttg ttaaacaccc tattaaaaca ggtatgctta
     7561 acggcaaaaa atatatggtc atggaaacta ctaatgacga ttactggaaa gatttcatgg
     7621 ttgaaggtca acgtgttaga actataagca aagatgctaa aaataatact agaacaatta
     7681 ttttcccata tgttgaaggt aaaactctat atgatgctat cgttaaagtt cacgtaaaaa
     7741 cgattgatta tgatggacaa taccatgtca gaatcgttga taaagaagca tttacaaaag
     7801 ccaataccga taaatctaac aaaaaagaac aacaagataa ctcagctaag aaggaagcta
     7861 ctccagctac gcctagcaaa ccaacaccat cacctgttga aaaagaatca caaaaacaag
     7921 acagccaaaa agatgacaat aaacaattac caagtgttga aaaagaaaat gacgcatcta
     7981 gtgagtcagg taaagacaaa acgcctgcta caaaaccaac taaaggtgaa gtagaatcaa
     8041 gtagtacaac tccaactaag gtagtatcta cgactcaaaa tgttgcaaaa ccaacaactg
     8101 cttcatcaaa aacaacaaaa gatgttgttc aaacttcagc aggttctagc gaagcaaaag
     8161 atagtgctcc attacaaaaa gcaaacatta aaaacacaaa tgatggacac actcaaagcc
     8221 aaaacaataa aaatacacaa gaaaataaag caaaatcatt accacaaact ggtgaagaat
     8281 caaataaaga tatgacatta ccattaatgg cattattagc tttaagtagc atcgttgcat
     8341 tcgtattacc tagaaaacgt aaaaactaat aaatcgtctt tatatttaat tattaaatta
     8401 acaaatttta attggcggat gaggtatcca gttacctcgt tcgccaatta tttttcgcaa
     8461 tataaaaagt cccacttaaa acaatcattt taagcgggac tttttatatt gagtaactaa
     8521 aattatttag ctgctacttc ttcgccattg taagaaccac agtttttaca tacacggtgt
     8581 gataatttgt attcgccaca gtttgggcat tcagtcatac ctggtactga aattttgaaa
     8641 tgcgtacgac gtttgttttt tctagtttta gaagttcttc tttttggtac tgccatgata
     8701 tatcctcctt agattataaa cgaaaaatac taaatgttag tttaattaac aacattatat
     8761 cattaattaa actacttatt gctctttatc atataattgt tgtaattttt gaagccttgg
     8821 atcaacttgt cgtgattctg aatcatcttg ttcttgctgt ttagcaagct catctaattg
     8881 atcctcatcg attacttccc aaccattacc tactgtcaac atttggtcac tttgctctga
     8941 ataagctctc attggtttct caataataac tatatcctcg acaatatcct gaagattaac
     9001 cataccatct ttaataatgt gatagtgttc atctacatca tcttgatcat cgttatactg
     9061 attgtaccct tctaaatcaa atacttctgt agtagttaca tctagtggga cttttactgg
     9121 tacaagagta cgtgcacaag gcattgtata cgttccagta atgtgaatat ccgcaacgac
     9181 ttctgttgac ttaatggtta actgaccttg gattgtaatt ggagataaat caattaaatc
     9241 taatgattct tttaaattgt caaaactcac cgtttgatca aattcaaatg gcttaccttg
     9301 atatttcctt aattgcgtaa ttgaccattt catatggctt cacctctaac aagcacaaaa
     9361 tttattttaa ctttctaaga acaagttgtc aagatttttt cttaacatat catgttctgt
     9421 tacaatatta ataatgaata ccgaaaggac gaattgcaaa tgaaaagcgt tggcttaatc
     9481 acagaatata atccctttca taatgggcat caatatcata ttaatcaatc taaaaaactt
     9541 acaaatgctg acgttactat tgcaataatg agtggtaact ttgtcatgcg tggcgaacca
     9601 gcaatctata ataagtttac tcgtgcaaaa atggcgttat caacagctga tttagttatc
     9661 gaactaccag caactgccag tttatcatct ggcgatcatt ttgccgaact agcagttaaa
     9721 gtcgcagatt atatgagtgt cgatacaatt gcatttggta gtgaaaataa tgatatcaaa
     9781 acattaaagc aattagcaca cagcattaat gaaattgaac aatctgaatc cttttcacaa
     9841 aaagtaaaag aaggtaaaag ctaccctcgt atcattagcg aattattaga gcatcatgaa
     9901 gcactagcaa gtcctaacaa catacttggt attagttacc tgaaagcaat tgctaaaaat
     9961 gctaaaaaca tcaatgcaat ttctatcaaa cgagaaaatg ctcaacatca tgattcatta
    10021 attcaacacc atcagtttgc aagtggtaca tctattagaa catcaatcat tagtcaagat
    10081 gatcattggc atcatgtggt acctaaagat attcaacact tgtacgtaac acctcatata
    10141 acattaaatc aaatatttcc atatttgaaa tatcaaatca tagctatgac tacagattca
    10201 ttaaaaaata tttatactgt tactgaaggt tttgaaaatc gtttgaaatc taacatttat
    10261 gaagcaactg attttcatca ttttgtaaaa ttactaaaaa caaagcgtta tacatatacg
    10321 catatccaaa gactgttaat gaatgtatta ttaaacatta aacctacaga tgttacgagt
    10381 aacattcacg cagttaaagt attagcgatg aacgaccgtg gcagacaata tttaaagcat
    10441 ttaaaaacag cgttcccaga aagacaatat ataaccaata ttaacaaaag caatgcacat
    10501 tacttcacaa atgaaatcaa agcaacacac atatacaatg caataagcgg gcaacaacaa
    10561 acagatttca acacgcctgt catacagcaa tatcgttaac aaatgaaaac agtattttag
    10621 gattgtaaac catgataaac ctaaaatact gttattttta ttacttaaat ttcttcttca
    10681 atgccttttc aacataaggt ggaacgaatt cagaaatatc tgctcgataa gctgcaactt
    10741 ctttaacaat acttgaactt ataaatgaat aattagtact agacatcata tataacgttt
    10801 caatttcatt gttcaacttt ttattcattg aagttaagcg taattcatat tcaaaatcac
    10861 tgactgctct taaaccacgt atgattgttt tagctcctac ttgttcacaa taatcgacta
    10921 gtaaaccact aaattgatga accttgacat taggtaaatg tttaacagat tgttcaatta
    10981 aatccatacg ctcttctaaa ctaaacgtac cttctttttt actattttta agaacacaga
    11041 catgaatttc atcaaatcta tctgtacttc tctcaataat gtctaaatga ccataagtaa
    11101 tggggtcaaa actacccgga atgaccgcta ttgtatgttc catgctattc tcccttttct
    11161 aataacaatg tgtctgtcaa cccataatgg taacgtttaa tcatattaaa cggttgatag
    11221 tctatttctt catgattgct aaattcacaa acgatgatac cattttcttt caataaatta
    11281 aactctgaaa ttagttttaa agctttatca atgagacctt tattataagg tggatctaag
    11341 aaaatgacat caaattgaat atcccgtttt gacaatgctt ttaaagctct atctgcatta
    11401 tttttataaa cttcagattg tgcctctaaa tccaaattcg caagatttga tttaataact
    11461 tttacagctt taaaattttg atcaacaaag attaccttat ccatacctcg agagagtgct
    11521 tctattccaa gcgccccact tcctgcaaat aaatctaaac ctatacctga cacatcatat
    11581 aaactattaa agataccttc tttaacttta tccatagttg gtctcgtatt acggccttcc
    11641 atactttcta aagctttact tttatgttta cctgcaatga cgcgcatgtt gttcacactt
    11701 ccaattcatt tagttattta atataattta ttgagaaaaa ggagaatgat aaaccaatga
    11761 aacaaacatt tattacactt ggtgaaggtc taacagattt gttcgaattc atgacgatga
    11821 ttgaatataa ccatcaacgt attgataaaa ttatctattt tcattcacca caagctgaaa
    11881 ataaaaagtc atctgtagca atcattatga accctacaac tggcaatcat ttccaagcat
    11941 tttatatcat gattaacgct attaaatatc catatccaga ttcaaataaa aagtttcaaa
    12001 tgattaatga ttgtgctgaa aaattcgaca taccaatttt aggtatcgat gtacagcccc
    12061 ctcaagcatt tcatgattta tcgttatatt ataattattt aattagtgtg ttaaggctcc
    12121 aaaaatggat accagaactt caataatatt aattatatat ttcgtgtttc tctttttcgt
    12181 aagttttctt taagtattta tatggtgagc catcaatgtg tctaacgtat ttaagttgca
    12241 ttagtttatg tacaatttga tcaacatctt gctcattcac atacataatt acaaatttac
    12301 gatctctatt tgaatgaacg atatgtccat attttcggat ttgtcgttca tgtttcatat
    12361 gttttaaata aactacaata ctagttcttg ggattaaatt catttaaacc tcactccatt
    12421 tattttcagc attaatcata tcatgtcttt attaatttta aaatattaag ctaccttaaa
    12481 tatgtcttgt gtaattgctc gataaatgta tttagattat attaaacatc attatattaa
    12541 ttcaattctc aagagcatca ctatttgatt aaccacacat ttcactgtag tcagaactat
    12601 gattttgtta agctataaaa gtaaacgagg aggaattttc tatgaaaaaa gtaaacagaa
    12661 acattttaaa tgcggtacta gtcggtgctg gatttgtaag tagtctatta atgatcaata
    12721 aaaataaagt gagtactaaa aagcaaacca tccctgcttt tttcaaaggc aatgcaccat
    12781 atatatttgc gcatcgcggt ggtatggctt taagaccaga gcaaacgcaa cttgcatttg
    12841 actatgctaa acaattaggc gttgatggct ttgaaacgga tgtgagactc acaaaagatc
    12901 aacagcttat cgtttttcat gacgcaactg ttgatagaac gacgaacggc tctggaaaag
    12961 ttagtgccca tacattagca gaattaaaga aattagacgc agcttatcac ttcaaagata
    13021 tcaatggact aacaccatat cgtggtcatg cacacacagc tatactaacc ttcgatgagt
    13081 tgctgaagca atatcctgat atgtatataa acgtagactt aaaagatgct cctgaatcct
    13141 atgaaggctc aatcgcacca caaattatgt ttgatacaat tgcagaaaat caagcatttg
    13201 accgcgttct tgtgacaagt ttttataaag aacaaatcgt tcgttttaat aaaattgcac
    13261 aaggatccgt cgcaattggt gctagccaac aggaagttac agaagcattt ttaaaatatc
    13321 atttattagg tggtagatac taccaaccat tagcacaaac gttccaaatg cctactcatt
    13381 ttaaaggcat tgatttgact tcatctcgtt ttatcaaatg gttaaatgat atgaatatca
    13441 ttccaggcta ttatggcgta aatagtatag atctgatgaa tgatttatac caaaaaggtg
    13501 cacacacgat tgtgacagat cgaccagatt tagcacaaca attcaaacaa acaatcccga
    13561 ataaataaaa acatcgagtc agatgctagt tagtggtttc aataaccacc tttttctgac
    13621 tcgatgtttt ttataatgaa caattacagg atccgcccgt cgcacaacca ctatgtgatg
    13681 tttgaaagaa tggattacct gtttctattt tgacatgctc tgaaacagcg tacgcaattt
    13741 tcgtaacaac ttcatcaatt aaatgttgaa gtgccatttc cctagctttg taatgcatca
    13801 caacgtcaag catctcatat gctcttttgc gttgtcttgt ctctaacatg actttcttat
    13861 aatcaggatg atatttcccg aaacgcatta cttcatcata tttctctttt gattttaaaa
    13921 atgattgata aagtaaatgt gcttcgtcat tgttttcgag cgcctgttta gcttgctcaa
    13981 atgatgcata aatatctgat gcaactatca tatcagctag ttcatcaata tcatctaata
    14041 tcgctagtga agcttcatta atcataaata cccctcattg ttatttttct aagaaaatta
    14101 gtgaataata atctctcatt acatctccac ccatctcatc gtattttgaa tttaatagac
    14161 gagatcgatg tatatctgaa ttcatccaac tatggattaa tgttggtaca tcattaaacg
    14221 cataaccaac attttgagca gttgttttat aagtaacgtg atttttatct aattgccctc
    14281 ttaatgcgtc ctctgtaaat tcaacactat cagaaccatt agaggtcgct tcatataagt
    14341 tattagatgc aatatgtgct aaatcgctat tgattttcaa tggttttaat ccttttaatt
    14401 ttctcatttc attcgttact tcataaagag aaattaattg atttggattt tgctcaactg
    14461 gacgcttatt atgctcttct gacgtagaat tagaatttaa ttgataaggt tcaatatctg
    14521 ctaacatttc ttttgttaaa aatcgtacac ttagcacctt tttcgattgt tgatcagaat
    14581 acacttgtgc atatatgtcg ccatatttaa tcagtgtttg tgtttttaaa tcttcatctg
    14641 aaagttcaaa ttcatatttt ttaccatcaa ctttaaagga cggttctgga ttaatacttg
    14701 tatgattaaa aatttctgca gaatgttgtc ctatttttaa cggactaaca ttgactttct
    14761 cacctgtagc atacactgaa acgatttctt cacgtttagt tgaaacaatg taataactgt
    14821 ttttgtcttt aaacacataa tttttgtaac catctctaaa agggtagaca cgatctgctt
    14881 gtccaaattt agaagtaagc actttaatat ctttacctac ccatgtacca acaccttctt
    14941 taggctttgg attttcagca tggttatttg atttattcac ctgttcagat ctatttactt
    15001 tattaccttt attcgggttc tctaatacat caaattttaa tctcggtgaa taaaaaagat
    15061 atattaaaaa tccaactaaa aataatacac cgacgactct tataattaac tttttcatca
    15121 atcaaccacc taaaaagtat taatactatt gtaaaaaaca acacattaat tagcaaattt
    15181 tcaacactga cataactgtg tcgtttcgat gaaacaaaat catcttcagg cataatttta
    15241 acgtcataaa gattttccct acactctata tcataaccta tctttatgtt ttcaggttga
    15301 atttcattat taggattaaa atatgtataa aaaaatggac agttaaggta tcaaatgaat
    15361 accatcaact gtccgactat tcttcatcaa aaaacctgat aaaacaaatt gccttatcag
    15421 attagtatca tttgtataag catattaatg accaaatgtt gctttaatca gtgatgtcgt
    15481 ttctccacct ggatataata catataataa taagtaaact gctacacctg taattgcagt
    15541 acagaaccat ataactgaag cgaatggccc gaatttgcgg tgtacattat atttatcttt
    15601 aaatgcagta ataatttgaa ctaggcctag aataccacca attgttgcta aattaatgtg
    15661 gaaaaataag aaaatcgtat aatatttctt aattgatgct ggtccgccaa atgctgtatt
    15721 accgatgaaa atcgttctac ttgcatagat taagaaaaaa gttaaagcaa aaacagctgc
    15781 agctaacata acatttttgt gtttatttat ttcccttttc caaataagtc tccaaccaat
    15841 ggcaattaaa attgcactaa tgacaataca tgtcgtacta atcgttggta aaattggaac
    15901 gcccatattt ttcatcctaa cttaattaat ctagatcaaa gtaagtaatg aaacaatcac
    15961 agctaacacg aaaaagatca ctaaataatt tagtgaatat ataaacattt gtgttgccca
    16021 ttttgtttga tctgaatttt tcttaaatgt tgttaaacct aatgcaatcc atcctaaatt
    16081 taataatgta gctaacacta cgaatacgac acctaaattt attagtaata aaggtactgg
    16141 caataaaata atcaaccaga taaacatact gacacgtgta cgtttaaagc ctttaactga
    16201 tggtaacatt ggaatatttg caagtgcata ttcatcttta cgtttaatag ctaaggcata
    16261 aaaatgaatt ggttgccaac aaaatacaac taaaaacagc gcaatcgctg ttaaactaat
    16321 ttgtccttca attgcaaccc atccaattag tggtggtact gctccaggaa aactcccaat
    16381 cactgtgttc catgttgtat gtcttttaga ccatattgag taataagaca cataacctac
    16441 aatccccata agaccaagta cgcctgatgg tatattcaat aaaaacaaac aaatttctcc
    16501 aactaacatc ataccaaaac ttaataataa taaattttga tctgtaattc tattatttac
    16561 agttggtcta ttttgtttac taggcataat acgatcaata tcttggtcgt aataattatt
    16621 taacgcacat gcgccaccca taattaaagt agatcctaat agcattaata aaatttgagg
    16681 tattgatgat aagaaggaat gatttgtcat tacaactgct agccatgcgc ccgcaaaagc
    16741 cggaattaag ttaccttgaa caagtcccat tttaattatc tgttgcaatt ctttgaagtt
    16801 aactctgcta atattttgtg acaaagtatg ctctttgctc ataatccccc tccttaaatt
    16861 tgttcatata agattatgat atcttagatt gcataaaaag actaggttta ataaaattaa
    16921 attgtgacaa attaacgaca atagaaaatg tcaattttgt gacacaaata acatttaatt
    16981 tattgctata atgtatatgt tagaaaattt taataagtag aatcatgcat ctaaaagaga
    17041 ttaatattta agcttcaaat ttgagtaaac gtggattaca taattatccc aataaaaaaa
    17101 tcattacgat taagttcttt ttatgtcgtc cacatacaat acttgtaaaa ttaaatcata
    17161 tttcctgcgt tggatcccat cttttcatat cctacaatca ggtcaattta tagtatcatc
    17221 tcaaatccgg ctattaattc taattctcag tgatgcgttt ttttatgatg gggtgtataa
    17281 attgtttggc aaaaagaatt taaaatggtt aggtgtcgta gcaacgttaa tgatgacatt
    17341 tgtacaactt ggtggagcct tagttaccaa aaccggatca gctgatggtt gtggttcttc
    17401 ttggccacta tgtcatggtg cgttgattcc agaattcttt cctattgata cgattattga
    17461 gttaagtcat agagccgttt cagctttgtc tttattaatg gtcttatggt tagttatcac
    17521 tgcatggaaa catataggct atattaaaga aattaaacct ttatcaatca ttagtgttgg
    17581 attcttatta ttgcaagcat taatcggagc tgctgctgtt atttggcaac aaaacgatta
    17641 cgttttagca ttgcactttg gtatatcatt aatcagtttc tcatctgtat ttttaataac
    17701 attgattatt ttctctatag atcaaaaata tgaagctgac gaattatata tcaaaaagcc
    17761 attaagacgt ttaacatggt taatggcaat catcatttat tgtggtgttt atactggtgc
    17821 actagtgaga catgcggatg caagtttagc atatggtggt tggccattgc cattccacga
    17881 tcttgtacca cattcagaac aagattgggt tcaactcacg catcgtatca tggcttttat
    17941 agtgtttacg attattatga ttacttatat tcacgctgtt aaaaattatc caaataaccg
    18001 tactgttcat tatggttata cagctgcatt tatacttgtt atattacaag ttatcacagg
    18061 tgcattatct attatgacaa atgttaacct gctaatcgca cttttccatg cattatttat
    18121 cacttattta tttggtatga caacatactt tatcatgcta atgttacgat cagtaagaag
    18181 tgacaagcaa taacaaaaaa gcaaaccgta attttaatgg cacgcccatt aaaattacgg
    18241 ttttttatat caatatttaa aattaaacct aagccatgta aaaacgagat tacacgtcaa
    18301 ttgttgtgta atctcgtttt attttaatca ttttagtcag ttgctttttc aatttcgatt
    18361 aataaatcgc ctgtcgctat tgtgtcacca ttatttacag ttacttgttt aatcacaccg
    18421 tcaaatggtg cttgaattgt tgtttccatt ttcatagctt cagtaattag caacggctgg
    18481 ttagctttca cagattcacc tacactaacc ttgacttcag ttactgaacc tggcatttgc
    18541 gcaccgatat gacttggatt actcttatct gcttttggct taacgttcgc atttgtatgc
    18601 acattctcat ctttaatgta aatacgtctc gcttgaccat tcatcgcata gtaaatcgtt
    18661 ctattaccat tttcatctgg ttcactaatc gtttctagtt taataattaa tcgtttaccc
    18721 ttatcgattt cgatttctac tgtttcacca ttacgcattc caaagaagaa tgtaggcgta
    18781 tcaagtaacg ataagtttcc gtattgattt ctagtttgaa tatattgttc atatactttt
    18841 ggatataata cataactaat aatatcttgc tccgtaacag gaccttgttg ctcttcttca
    18901 agcaactcac ggactttttc aaaatcaact ggctctagat attcacctgg acgagctgtt
    18961 agtgcttctt ggccttttaa aataaccgct tgtaaatctt tattaaaacc atttacaggt
    19021 tgtcctattt cgcctttgaa gaacgacact actgattctg ggaaatctaa tttatagcca
    19081 tctgtaatca cggattgttc atcaagatca ttttgtacca tataaagtgc catatcacca
    19141 actactttag acgatggagt tacttttacg atatcaccaa ataagaaatt cactctgcga
    19201 tacatatctt tgacttcatc aaatctttcg cctaaaccta aacttttagc ttgttgactt
    19261 aaattcgaat actgtccacc aggcatttca tgttgataaa tttcagtatt cggtgatttg
    19321 atatcacttt caaagtctga ataataagta cgtacagttg accaataatg actaagtgac
    19381 tccatacctt caatatcagt tctaaggtgg cgtgggaagc catttaatgc ataatataac
    19441 gaattggcgc ttggctgact tgttaaacca ctcattgaag caacagcagt atcaatgata
    19501 tcgacaccag catctattgc ttgtttgtat gttaataaac cattaccact tgtatcatga
    19561 gtgtgaagat gaattggtaa atctacagct gattttaact caccaatcaa ttcgtaagcg
    19621 gctttaggtt ttaataagcc tgccatatct ttaatcgcta aaatatggaa accttcacgt
    19681 tctaactctt tagctagttt gacataatac tctaaagtat aaatgtttga tcgctcagga
    19741 tttaaaatgt cacctgtata acaaatagta ccttcagaga ttttgcccgc ttcttgtact
    19801 gcttcattgg caactttcat ttgatctacc cagtttaaag aatcgaaaat tctaaagaca
    19861 tctatgcctg ctttagcact ttcttgtacg aatttatgaa taacattatc aggatagttt
    19921 ttataaccaa ctgcgtttga agcacgtaac aacatttgga ataatacatt tggaatagct
    19981 ttacgtagac gttcaagtcg ttcccatggg ttttccttca agaaattata tgccacatca
    20041 aatgtagcac cgccccacat ttcaagtgag aaaccatctt taaatacgtc cgctgttttg
    20101 gatgcgatat taatcatatc cttagttcta actcgtgtag ctaataatga ttggtgtgca
    20161 tctctaaagg ttgtatctgt tagtaaaaca tcatcctgct ttttaaccca ttcagctaca
    20221 ccttttggac ctacttcatc aagcaattgt ttcgtaccac taaatgaagc gattttactt
    20281 gaagatacag ttggaattga tgctaattca tagtctggtt tcggacgttt ctcaacattt
    20341 gggaaaccat taattgttac attacctata tattctaatg ttttagtacc tctatctaga
    20401 gacggctgaa tgtcgaaaag ttctggtgtt tcttcaataa atttagttgt gtaatcacca
    20461 cttgtgaact ttttattctt cattacatta attaagaatg gaatattagt tttaacacca
    20521 cgaatacgca tttctcgtaa tgaacgtacc attttttctt ctgcttgttt aaatgatatc
    20581 gcgtgtgtag ataattttac gagtaatgaa tcataataag gtgatatctc agcaccttgg
    20641 aaaccatctc cagcatcgag acgtacacca aagccaccgc ttgaacgata agcaatgatt
    20701 gttccagtat ccggcatgaa atcatttaac ggatcttctg ttgtaatacg acattggatg
    20761 gcatagccca atgttgtaat atctttttgt tgcggcatat taatttcttc accaaataaa
    20821 tcggcacctg ctgcaactaa aatttgtgtc ttaacaatat caattcctgt taccatctct
    20881 gtaattgtat gctcaacttg tacacgaggg ttaacttcta taaagaagaa ttcgtcacca
    20941 gatactagaa attcaacagt acctgcattg acatatttaa tattttccat caattgaatt
    21001 gcagcatcac aaatacgttg acgtaatgtt ggtgataatc caactgatgg tgcaacttct
    21061 acaacttttt gatgacgacg ttgtactgaa caatcacgtt caaataagtg tacgatattt
    21121 ccatattcgt cacctataac ttgtacttca atatgctttg gattatcaat gtatctctct
    21181 atgtaaactt cactattacc aaatgatttt tcagcttctg attttgctct atggaaagca
    21241 tcttctaatt cactttcttc acgaacgatt ctcatacctt taccgccgcc accacttgtg
    21301 gccttaatca ttagcgggaa accagcttct tctgcaaatt cttttgctaa ttcatatgat
    21361 ttaattggac cgtctgtacc aggaataact ggtaaatctg ccttgatagc cgttgtacga
    21421 gctttaactt tatctccaaa catatctaaa tgttctaaat gaggaccaat aaatttaatt
    21481 ccttcttctg cacaacgacg cgcaaattgt tcattttcac ttaaaaatcc atagccagga
    21541 tgaatcgcat ccacattcgc ttgttttgct acatcaatga tacgctcaat atttaaataa
    21601 ctttcagcag gacctaaatc acttccaact aaataggatt catctgcttt atatctatgt
    21661 aatgaacttt tgtcttcatt cgaataaatt gcaactgtgc tgatgtctaa ttctgccgcc
    21721 gctctgaata tacgaattgc aatttctcca cggttagcaa caagtaactt ttttatttgt
    21781 ttcaatagcg atacactcct caaactatta gaattttcta actaattaga taataaaatt
    21841 ttatcttaaa gcgctctgtt ttgctatagt tatgtttcaa attttcaaaa tttaacattc
    21901 ttgagacaat taaaacctcc gcttcagaaa taataatttc aaaaatgact atgcaacaac
    21961 aggtagttca cgtttttgtt gtgaaacatt ttcgatttct acaactctaa aaaattaaaa
    22021 ataaaattgc aaaacatcaa catttattat caatagcgat aactttatct tatcatcatg
    22081 attctaattt cgccaccaca tttagtaatt tttagtcata aaatttagtt ataattatac
    22141 gttgttttgt ttataaaatt tgataatcat gagtaatctc gtaatatcaa aacaaaaagg
    22201 aagttaagcg ttgtttggtt gcctaacttc cgttattgaa ctcatcagtc caatttgaca
    22261 aagagtatat aaatagccaa tgtttagcat tgcttaatga cagaagtcat ctcgtactaa
    22321 atattggcta gtattttttt aattaaattg tcttcttata tcaacttttt gttgttgttt
    22381 ctttcgttgc tggtctactt tgatttgttt acctacaatc agaagtaatc ccatagcaat
    22441 acttaaacta atcattgatg atccaccaaa gctgataaat ggcaatggca caccagttaa
    22501 tggaattgtt gccgaaatac cgccaatgtt tacaaacgtt tgacttccaa agtatgtggc
    22561 aatcccaaca cacacaagtt tataaaaata tgatgatgtt ttatttgcaa actggaaggc
    22621 acgatataca ataaagaact ctaaagtaat aactagcaat cctccgatta aacctaattc
    22681 ttcgcaaata attgcaaaaa taaaatctgt atgtggttct ggtaaatagc ccaatttcat
    22741 tgcactattt cctaatcctt ttccaaatac gccaccgtta cctatcgcaa gcaatgaatt
    22801 ggaaatatgg tatccagttc ctgattcgaa ttggaatgga tctgttagcg tactaaatct
    22861 ggcagttaaa taacttggta accaaccagc cattaatgca atgacaaata ctactaagaa
    22921 tcctagtact gctggtatac caaatcttag gactttgttt accccaattc ctgaataaaa
    22981 aatgatcgca actaaaataa ttaatattag taatgtttgc cctacgtctt tttgtaggaa
    23041 aactaaaaac gtacaaccta atgctaatac aataggactt aaaattaatt ttggtttact
    23101 taatactcta ggcatttttt tactgatcat aaatgggata tataatatga ttgcaatttt
    23161 taataactca gatgcctgta agttcataaa tcctaagttt atccaacttt tagaaccatt
    23221 aatatcttta cctattacta acgtcagtaa taatagtgag acgatagtta taatcatacc
    23281 tttttgcact ttaatattac tcagtaattt aacattcatt aaaaatgcaa taaaaaatac
    23341 aattataaaa ctcattatga catatgctaa ttgtcggttg taaaaatacg ttcctggaac
    23401 atcgatacca ccagtcaatg tgcctttagt tgctggaacc atacttgcac tatataccat
    23461 cactaatcca attaaactca atacaatata tgtaactaat aacggataat caataaactt
    23521 tgaggtttta ccaatatacc gtaaaatact tctaaaattc ttcatatatt cagtcatcca
    23581 attctacaat ttatacttca aatgatttcg ttttatactt taattttaaa aatccatgtt
    23641 acttattata tacatgacac agtcataatc acaaatatta acaggtctct tgaggtaatt
    23701 tatagaatat ttgtttactt aatatcgtga attgctaaaa cccaataaaa gtgactgaca
    23761 tgtcatcaag aagacaacat aacagccact ttaatcagta attaaactat aaatataaaa
    23821 tcggcgcagc tcaattgtcg atatctcacg ccattttaat atgcttatat attaaacctt
    23881 aaacaagtgt aaaagcttca tgtaattttg aaagttcttt ctcaagacgt aacataattt
    23941 gtttgccatc ttcgcggtca actaaaccta atttaacagc aaaatcaact tctttttgaa
    24001 gtccaaacat ttgtgtatct agtacttctt catataatgg gcatgaaggt aatgttaaat
    24061 tatctagttg aactttaatc agatgcaaga tttcatcagc atctttagtc aattgtttca
    24121 aagctgcatt tttcattgtt gcttgtttcg ccatgacacg ctccccctct atataaattc
    24181 tcaagtctta ctaaaagttt atcttactaa atattaaaaa gcaagtagat ttaaaatgtg
    24241 tcgttagcaa tttgtgttaa aataatgcta tatacattga tagggagtgg gaaaatgata
    24301 caaattaaag gagccgtaaa gtttcctata acattagata gtacgacttg gattttcgat
    24361 gatagaaaag ttagcatcga agatttagaa aaaggtgtat ttgatggttc aaaaccaatc
    24421 gactttgaag ataatcatga atggaatcgt gctatcctag aaggacaaac taatccgcct
    24481 actttaaatt cagaaataaa atataaaaaa cgtgccatgt tagaaggttc atttgtcatt
    24541 aatatgacac cttttttcaa acattccgaa ccacatgact ctgcaactac tattcgatta
    24601 tcaaatgagc atgatcattt agatataccg atagatctat taccctattt atttttccaa
    24661 tttgcaaaag acggtaaacg actttacagc gataatggtg tagatagttt tatttacaat
    24721 caagaagatg gttattcata tcgaatcaac catgtaactc atattgaggt gatttaattt
    24781 gagacaagtg caatgcatta tctgtgatgc aaaagtattt atagatgagc gcacaactga
    24841 gtctaaacga cttaaaaaca atcctattcg aacatttatg tgtgatgatt gtaaaagtcg
    24901 attagacaca cctaaacaac gtgcgcaaca ctatccgctc gattaatatc tgaataaaca
    24961 agttagaaat ttgaaaatgt gaaagtaaca ggaaataata gcatttaaac actttttcaa
    25021 atattatatg ccaaaagaga agctaagcaa ctttcatcgc tcagcttctc ttttattatg
    25081 ttaaattatt cgttttcttg catcatttgt ttgatacgct ttgcttcttt ttcacgaaca
    25141 tttttgttta aaattttctt tcttaaacgt atgctttctg gtgtaacctc aacaagttca
    25201 tcatcattaa tgaattgtaa cgcttcttcc aatgttagaa tacgcggtct attcattgtt
    25261 tgtgtttggt ctttcgttgc agaacgtacg ttagtttgat gttttgtttt agtgatgtta
    25321 acagttaaat cattttcacg attatgttca ccaacaatca taccttcata aacttcagta
    25381 ccaggttcca tgaagtttac acctctatct tcaagtccca aaatggcata agtacttgca
    25441 gaaccttgat ccattgaaat taatgcacca ttacgacgac cgccaatttg tgctttaata
    25501 cgtggtctaa attcttcaaa tgtatggtta ataataccgt aacctcttgt cattgacata
    25561 aattcagtcg tataaccaat cataccacga gccggtacat taaagattaa acgtgtaagt
    25621 ccattatcag ttgtagtcat atcaaccatt tcacctttac gtgcacctaa tgattcaata
    25681 acagcacctg cattttcttg tggcacttca cattgcacac gttcaaatgg ttcacacatt
    25741 acaccatcta tttctttaat aattacttgt ggttttgaaa cttgtaattc ataaccttca
    25801 cgacgcatat tttcaataag gattgataaa tgcaattcac cgcgaccagc aactacccat
    25861 gtatctggag aatctgtgtt agaaactttc aaagatacat ctgtttctaa ttgttgattt
    25921 aaacgttctt gaatttgacg cgctgttaca aagtcacctt cacggccagc aaatggagaa
    25981 ttgttaactt taaatgtcat ttcaagagta ggctcatcaa tacgtagaac tggcaatgct
    26041 tcttgatggt catgtggtgt tacagtttca ccaacattaa tgtcttccat acctgaaaca
    26101 gcaattaaat ctccagcttg tgcttcttca atttctaaac gttttaatcc aaagtaacca
    26161 aagattttag ttacacggaa gtttttcact gtaccgtcta atttaattag tgatacatta
    26221 tctccgacac gcattttacc tctgaataca cgaccaatac caatacgtcc aacataatca
    26281 ttgtagtcca acaatgctac ttggaattgt aatggctcat cactgttatc aattggagct
    26341 ggtacataat caataattgt ttcatataat gattgtaaat tatcatcttg tttttcagga
    26401 tctaagctag ctgttccatt tactgctgaa gcataaacaa cagggaattc taattgttca
    26461 tcgtttgctt ctaattcaat aaataaatct aaaacttcat ctacaacacc ctctggacgt
    26521 gctgatggtt tatcaatttt attaacaaca acaacaggtt tcaggttttg ttctagcgct
    26581 tttttaagta caaaacgtgt ttgaggcatt gtaccttcat acgcatctac tactaagaca
    26641 accccatcaa ccattttcat aatacgttct acttctccac caaagtctgc atgtcctggt
    26701 gtatccaaaa tattaatacg tgtaccttta taatcaacag ccgtattttt ggctagaatc
    26761 gtaattccac gctctctttc gatatcgtta gagtccattg cacgttcatc gacatgttca
    26821 ttttctctga atataccaga ttgttttaac aactcatcta ctaaagttgt tttaccatgg
    26881 tcaacgtgag caataattgc tatattgcgg acatcttctc ttttattagt catataaata
    26941 gtttccttct ttcttaagta aaatcccact tttaattaca acttttttat tatatcatat
    27001 aattgagaaa agataagcaa aaggtgggga taggatgaaa caaaaaaaat ctaaaaatat
    27061 cttttgggta ttttctatat tagctgttgt tttcttagta ttatttagtt ttgctgttgg
    27121 tgcatcaaat gtaccaatga tgattttaac atttatatta ctcgttgcaa cctttggaat
    27181 tggatttact acaaagaaaa aatatcgaga aaacgattgg ctataagtta tatcgacaat
    27241 aacacgcgtt gatttaagac cacacatgtt catgatgaac atttaactgg ttttatattg
    27301 gcgcgtgttt ttattattta aggtataaaa gcgtaatatg ttacctaatt gttaggacta
    27361 catattacgc ttttggtaat tattttgatt ttcttttaaa tcgttgttca tgtaattgta
    27421 ttaacgcatc atggtggggc tctaaataat cattgctaat ttcttgatgg agaccacgat
    27481 ttccaactaa gattgaattt ggaccactaa ttgttaatgg ttctcctagt aaattggaag
    27541 cttgtccatt tacttcatat aaaataacca atccgccagc aaaatcccat ggttgaagtc
    27601 ttggcgtcat atatgcttct aaattacctg tagcaactga aacgatttca agcgctgcac
    27661 taccatatgc ccttgcactt ctagaatcat taacaatttc tttaaagatt tctcctaaaa
    27721 ttggtttagt taaccagttc ggattgatcc caataatgct ttgtcttaga ttagaatcat
    27781 tcaatggttt caagggttgg ctaccacgat atgcaccttc ccctacttta gcatgatata
    27841 agacatcagc cataacatca tatacaaaac ctgcataagg tttaccatcg atataaatac
    27901 caattgaaat tgcgaaattt tcttgttgat gaacaaaatt caatgtaccg tctattgggt
    27961 caacaaccca taccgtacct ttggaagtat cgatgtcatg accatgccct tcttcaccta
    28021 atacttgatg attgggatat gtttctaaaa ttgtatcaaa aataaaatct tctgttgctt
    28081 tatctacatt tgtaacaagg tcattcggat ttgactttgt ttcaattgtt aaattttgct
    28141 ccatcaattg tttaattcta attcctgctt cttgaataag tccttgggca aatccataaa
    28201 gtgccataac atcatctcct aacatcttta ttatacatca acattttata aaaataactt
    28261 cttatgataa aaatgaaaat actaatttta atgagagcgt cttaagccgc aattgataaa
    28321 acatatgcta caatattttt aaacactata caggaggtac tctatgacaa aatatacatt
    28381 taaacctaaa gatttcaaag cgttcaacgt agaaggctta gacgcacgaa tggaagcttt
    28441 aaacgaatac atacgaccac aactccatga attaggagaa tattttagtg acttctttac
    28501 aagtcaaact ggtgaaacat tttatcctca cgtagcaaag catgctagaa gaagtgtgaa
    28561 tcctcctaaa gatacatggg ttgcttttgc aacaaacaaa agaggctata aaatgttacc
    28621 tcatttccaa attggtatgt ttgaagatca actgtttgtt atgtttggaa tcatgcatga
    28681 agcaaaagat aaagcaacac gtgcaaaagt ttttgaaaga aactttaaag ctattcaaca
    28741 attacctgat gattatcgtg tttgcttaga tcatatgaaa cctgataaac catttattaa
    28801 agatttaacg gatgatgatt taaaagaagc gatacaaaga gccatcaatg tgaaaaaagg
    28861 tgaattcttt atagcgcgtg caatcacacc acaagataaa agattaaaaa gtgacaaagc
    28921 atttattgca tttttagaag aaaccttcga tcagttctta ccattttatt ctgcataaat
    28981 aactttgttt aaataataga gcacttaatc acatccatga tttcgtgctc ttttttctta
    29041 atattaaatc gaacgttcaa cataataatt catacttttt aaaaaattaa aataaattta
    29101 ggttgacctg aacattttat taggttatta tattgtccat aagaagtaga ggtgagtcaa
    29161 aatgaataat aaacgacatt caacaaatga acaattaagt ttagacgaaa taaacaatac
    29221 aattaaattc gatcatcgca gttcaaataa acagaaattt ttatcatttc ttgggcctgg
    29281 gttattagtc gctgttggtt acatggatcc cggaaactgg ataacatcaa tgcaaggtgg
    29341 cgcgcaatat ggctatactt tgctattcgt aattcttatt tcaagtttat cagcaatgtt
    29401 acttcaaagc atgacagtga gattaggaat agcaacaggt atggacttag ctcaaatgac
    29461 acgacattat ttatcaagac ctattgctat aatcttttgg atcattgcag aactagcaat
    29521 tatcgctaca gatattgctg aggttattgg tagtgctatt gctcttaatc tcctatttaa
    29581 cataccttta atcgtcggtg cactaataac tgtacttgat gtatttttac tactatttat
    29641 aatgaaatat ggttttagaa aaattgaagc tattgttggt acattaattt tcacagtgtt
    29701 attcatcttt atatttgaag tctatatttc atcaccacag ttgaatgctg tgttaaatgg
    29761 atttatacca catagtgaaa tcattacaaa taacggcatt ctctatattg cattaggtat
    29821 tattggcgct acaattatgc ctcataattt gtacttacat tcatcaattg tacaatctag
    29881 aacatactca agacataaca atgaagaaaa ggcgcaagcg attaaatttg ctacgataga
    29941 ttcgaacatt cagttatcaa tcgcatttgt agtcaattgc ttattattag tgttaggtgc
    30001 atcactattt ttcaactcaa acgctgacga tttaggtggt ttctatgatt tatatcacgc
    30061 cttaaaaact gaacctgtac taggtgcaac aatgggtgca atcatgagta cattatttgc
    30121 agttgcatta ttagcgtcag gtcaaaattc aacgattact ggtactttag caggacaaat
    30181 tgtaatggaa ggatttttaa gattacacat accaaattgg ttaagacgtt taattacacg
    30241 ttcccttgct gtcattcctg ttatcgtatg cttaatcatt tttaaaggta atgcagctaa
    30301 aattgagcaa ctacttgtat tctctcaagt atttttaagt attgctctgc cattctgttt
    30361 aataccatta caattggcta ctagtaataa agacttgatg ggacctttct ataataaaac
    30421 atgggtttat atcatttcat ggacacttat tattattcta agtattttaa atgtctattt
    30481 aatcgttcaa acattccaag aactacaaag ttaaaaataa aaccattaaa atgctggttg
    30541 tatctaataa ttaacaactt aacattttaa tggtttttct tttgctatga acatattaat
    30601 gataaatata ctcatcgtcc ttatgtgtag tttcttttga ttctttttta gaaatcaaca
    30661 taatcatcac gataacccat aagacaattg caattaaatt aagagccatc aaacttacaa
    30721 ttatcgcgat aacaaaaagt ataatcgata attttctacg tcctttaata caaattgtcg
    30781 caaatatacc ataaagtgtt gaaattaata aaataattgc taatgcaccc attgttgtct
    30841 gaagcatatc taaagaaacc ttgccaccgt tctgagataa ttgcttcgct aattcttcac
    30901 gttgtgatgc attaccactt tttaaagaaa aataggatag ccccataact aataaataaa
    30961 taatactaat actatttgca atccaagcca tgattaattc aggttttcta ttcatcttaa
    31021 aatcctttct ttgtttaata ttttaataag tatactacga gcctatagat atacgcacat
    31081 taaattactt gtatattaac atatttttca tctaattttt ataataaaca tctgaattca
    31141 gattgatgat aatttaggtg tatagtaatg agaaatcgtc atttaaaatt atgacaccta
    31201 aacactttaa ttaaacgtta tcgataatta acctcatata aaaacccaga ttaataagca
    31261 aaagacctct gcttactaat ctgggtatca tcaatattta ttcttgctct tttttgtcct
    31321 taacttcact tgaattgcgt tcttcagaaa tatcattgtt gtaaatttga cgtcgctctt
    31381 caaaattcat tcgacgttga ctaactgcgc taggttgatt tttcctacgt tctttcattc
    31441 gttggttata agctttacgt ttttcttttt tcttagcttt aatttcagct ttttctcgct
    31501 tttgttgtgc tttcaattct tttggatcaa cttcaggttc tgcataataa ccatcatctt
    31561 caatacctag ttcttcagta cttttttgtt tgtatttagc ctgtctggat aatactgatg
    31621 gttcttcctc agtgcgctca tcatgattat attcactttc aactgcacgt ttaggaaatt
    31681 gatcatattt atctctttcc tgttcttgat catatacagt atttgattta tccaattctt
    31741 tttcatattt aggcacatca taataatcat ctttcggttg ggggcgctca tacacaggtt
    31801 cacgataatc ataaatcggt ccgttatact cttcataata ttgtggcgca accatttcaa
    31861 ctttatcttt tcttataaat agcatcatcg caataataaa gaataatatc ggaatgatta
    31921 aagtaacgat aagtaaaact aaaggaatcg ttacaattgc tgatattaag aataagaaac
    31981 ctgacaagat tcttatattt atagatatga gagctaaaaa tgaaatcaac aaacaaacaa
    32041 taaagtaaac gataatagcc catataccat tttgtaacca tattacaaat tgtgttgtat
    32101 tatagccatt tccagctaat aattgctgga taaatgcatt attgtttaat gtattttcaa
    32161 gattagcaat cgatgtgtta ttactgaatg aaacaagtgc tataaacatc gtaatgacag
    32221 taagcactaa taacatcacc caacataacc aacctagaac tttttcagtt aatctactta
    32281 ctggacgttt aatttgagta aattgttctc cagtcattcg ttacaactcc ttatagtact
    32341 tatcccgtta ttataactaa atatacagta aataactact atttatgatt ttattttaat
    32401 gacattttga aattcaaaaa gttttcattg tattcactta aaacttcagg tcctaaatct
    32461 ttataaactt caaggcgttc ttgctctttc ttagtcggat aaaaacgatg gtcgtcttta
    32521 atctctttag gcaacaattg tcgagcagcc ttgtttggcg ttgcatagcc tacgaattct
    32581 gtattttgct tgttattttt agcatctaat aaaaaattca taaatttata tgcaccctct
    32641 ttattttgtg ccgtttttgg aattaccata ttgtcgaacc ataaattcga tccttcttta
    32701 ggaataacat aattatattt atccccttct tgcactagag gtgctgcaac accactccaa
    32761 acaaccgcta tgttaccttc attttgttga agcatcatgg taatttcatc acctacgaca
    32821 cctcttactt gtggtgctag tttggttaaa tctcgctctg cttcttttaa atggtgcgaa
    32881 ttacggtcat taagattata cccaagttta ttcaaactca tgcctataat ctctctagca
    32941 ccgtcaacta gtaaaatttg gtttttaaat ttaggattat acaatgactt ccaactatca
    33001 aatgattcat ttggatactt ttctttatta tataaaatac ctacagttcc aaagaaataa
    33061 ggtaaagaat atttattgcc tctatcaaat gacatattca tataatctga atctaaattt
    33121 ttaatattag gtaccttatt atgatctatt ggtaacaata aatgatctct tttcaatttt
    33181 tgaactgtat attcactagg aaaagcaaca tcataatgtg taccgccatt gcgaattttg
    33241 gcttccatcg cttcatttga atcgaaagtt tcataaacga cttgaatgcc agtttctttt
    33301 tcaaatttct taattaactc tggatcaata tattcgcccc aattgtatac gtaaattttt
    33361 tgatttgtat gcacttgttc tttagattta aaccaatgac ttaaagtaag acaaagcata
    33421 cccacaacta atgcacctat aatgagttgt aaaaaacgtt tcattatttt acacctcgct
    33481 tgattagttt tttcttattt atcacgtatt gaatcaaata atatcctagt attcctaata
    33541 caataacagc aaacagtaat gttgaaatcg cattaatttc catactaatt ccttttctcg
    33601 ccatagcata aacttcaact gataacacac taaagccatt accagtaacg aagaaactta
    33661 ctgtgaaatc gtctagtgaa taagttaaag ccataaagaa tcctcctata atagaaggta
    33721 aaatattagg aataataatg ttgcttaata attgtggttc agtcgctcct aaatctcttg
    33781 cagcatttaa catattatta ttcatttcat acagttgtgg taagacgata atcacaacta
    33841 taggtatgca aaatgcaata tgagatatta gaactgtcca aaatcctaaa ccaagaccag
    33901 taaaatggcc aatcgttgta aacataatta agaatgatgc acctatgaca acgtcggatg
    33961 ataccatcaa gacattattc aatgttagta aagttacttt aaacttttta tttcttaaat
    34021 aataaatagc aatggcacca aatgtaccaa taactgtaga aattgaggct gctaaaagtg
    34081 ctacagctat cgtattaaaa atgaccgaca ttaaatgatc attttgaaat aatgattgat
    34141 aatgctctaa tgtaaaatgt tcaaagtgaa tcatattacc agccgaattg aatgaataga
    34201 acattaaaaa gaatattggg atgtataaaa tcgctaaaag tatcccgata tacagctttc
    34261 cataccattt catatgattc accctctccc attagatgat tttgtaatga ttaaaatgaa
    34321 tgccataaat acaattaaga atatagctat agttgatccc ataccataat tttgaattgt
    34381 taaaaattgt tcctctattg ccgtacctat atttatgact ttattacctg caattaatct
    34441 tgtaatcata aataatgaaa gtgatggaat aaaggttact tgaatcccag tcataacacc
    34501 ttcttttgtt aacggcatga ttacttttct aaaagtatag aaaggactgg cacctaaatc
    34561 acttgaggcc tgcaataaat tattaggaat tgctttcatg ctattaaata taggtaaaat
    34621 cataaatggt atataaatgt aacttgccac tactaaaaac gcaccagttg taaataacaa
    34681 attgaatgat ggtaaattaa ataagtggaa aaattgatta atcacgccat catgacttaa
    34741 taaacctata aaagcatatg tctttaacaa taaatttatc catgttggaa taatcattat
    34801 cattaataag atattttgaa atttcgaacg agtaatataa taggcagctg gataactgat
    34861 agtcaaggta ataattgtta ttaaagcggc atataaaatt gaatatgcaa acattttcaa
    34921 atattttgta gtaaaaattt gttgataatt cgtgaaacta aaatgtccat tgatatctaa
    34981 aaatgaaaaa taaatgagta atacaactgg tacaataata aatataacca tccatagtaa
    35041 atacgggatt aagagaaatt tattagtatt acgcattgtc tacttcctca tagctttcaa
    35101 tacgtttatc aaattcttct tctgtttctc caggaaccat aatatgaatg gcttcaggat
    35161 caaaataaag accaacttca ctgcctactt cagctttttt agtcgtttgt attacccatt
    35221 cataaccttt attgtctata caacatattt catagtggac ccctctaaat aacatagaat
    35281 caacagttgc tttaaataat ccttcttcag ctttgattaa tgatatatct tctggtcgaa
    35341 taacgacttc tactttttta ttttcaggaa tacccatatc gacacattcg aaatcttgcc
    35401 cataaatatt cacgacataa tctctaacca tgcgcccttc aacaatatta gattctccaa
    35461 taaaatcagc tacaaatcga ttcactggtt cgtcatatat atctgttggt gtgccaaatt
    35521 gttgaatttt cccatctttt aatacaaaaa gaaagtcact taatgctaac gcttcttctt
    35581 gatcatgtgt tacaaatata aatgtaatac ctaatctaga ttgcaattct cgtaattcat
    35641 attgcatttc agtacgcaat ttcaaatcta atgcggataa agattcatct aacaataata
    35701 tttctggttc atttacgata gcacgtgcaa ttgcaacacg ttgcttttgt ccgccactca
    35761 tttcattaat atttcttttt tcataacctg aaagttttac taattttaat gcctcagtta
    35821 ctttttgatc aatttcggtt tttgataatt tttttaattt caaaccaaaa gcgatattat
    35881 catagacgtt taagtgtgga aataatgcat aatcttgaaa gactgtattc actttacgtt
    35941 tatttggtgg taaattacca attggtttgt tttgataaat cacttcacca ctgtcaggat
    36001 attcaaaccc tgcaattaat tttaaaattg ttgttttacc acaacctgaa ggacctaata
    36061 atgtataaaa gtatcctgat tcaatatcaa tatctatgtc atctaagata ttaagatcat
    36121 catagctttt actaactgat tttaatgata ataacggttc caaataagat cccccctata
    36181 aatatgaagc tgtcgctaca ataagtattc gtgtttcatt gtttgattcg tttgacaaac
    36241 gatgattatc tcgtgcttta aaatacaaaa cgtcttcttc ttgtgcttga tatatctctt
    36301 tgcctaaatt aagtgttatc tgacctgaca tacaataaat aaacgtatca gactctgatg
    36361 gattaaaatt tttatatgat gctccaggct ttaaagttaa tattaatggc tccatatcat
    36421 attcatttga ctttgaaact aaccaattta atatataacc ttcatcatac tcatcataaa
    36481 taacttgttc ttccttcttg tataatactt tttcattttc actgtcttta aaaaattcac
    36541 ttggcgtcgt tcctaacacc tctataatat ttaagaaagt ttccatactt ggtgaggcat
    36601 gttcactttc tatttgtgaa atgtagcctt tcgataagtc tgtacgttca gcaagttctt
    36661 cttgcgttaa atttttaatt cttctaagat ttttaatttt attacctatg ttcatt
//
LOCUS       DAMZLE010000011        34939 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMZLE010000011 DAMZLE010000000
VERSION     DAMZLE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.011"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            4..450
                     /locus_tag="POB92_000547"
     CDS             4..450
                     /locus_tag="POB92_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6578071.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            666..1094
                     /locus_tag="POB92_000548"
     CDS             666..1094
                     /locus_tag="POB92_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDI6578072.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1201..2370)
                     /locus_tag="POB92_000549"
     CDS             complement(1201..2370)
                     /locus_tag="POB92_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDI6578073.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2739..3654
                     /locus_tag="POB92_000550"
                     /pseudo
     CDS             2739..3654
                     /locus_tag="POB92_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
     gene            3633..4373
                     /locus_tag="POB92_000551"
     CDS             3633..4373
                     /locus_tag="POB92_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6578074.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQVSKLDKKISDLQLIRRSV
                     CEFINGHSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4631..5284)
                     /gene="nreC"
                     /locus_tag="POB92_000552"
     CDS             complement(4631..5284)
                     /gene="nreC"
                     /locus_tag="POB92_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDI6578075.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5307..6341)
                     /gene="nreB"
                     /locus_tag="POB92_000553"
     CDS             complement(5307..6341)
                     /gene="nreB"
                     /locus_tag="POB92_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDI6578076.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6365..6817)
                     /gene="nreA"
                     /locus_tag="POB92_000554"
     CDS             complement(6365..6817)
                     /gene="nreA"
                     /locus_tag="POB92_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDI6578077.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6837..7514)
                     /gene="narI"
                     /locus_tag="POB92_000555"
     CDS             complement(6837..7514)
                     /gene="narI"
                     /locus_tag="POB92_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6578078.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7507..8082)
                     /gene="narJ"
                     /locus_tag="POB92_000556"
     CDS             complement(7507..8082)
                     /gene="narJ"
                     /locus_tag="POB92_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6578079.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISTDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8075..9634)
                     /gene="narH"
                     /locus_tag="POB92_000557"
     CDS             complement(8075..9634)
                     /gene="narH"
                     /locus_tag="POB92_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6578080.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9624..13313)
                     /locus_tag="POB92_000558"
     CDS             complement(9624..13313)
                     /locus_tag="POB92_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI6578081.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQGMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILKRAINEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNENEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13621..14598)
                     /gene="cobA"
                     /locus_tag="POB92_000559"
     CDS             complement(13621..14598)
                     /gene="cobA"
                     /locus_tag="POB92_000559"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6578082.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNNTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14589..14903)
                     /gene="nirD"
                     /locus_tag="POB92_000560"
     CDS             complement(14589..14903)
                     /gene="nirD"
                     /locus_tag="POB92_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDI6578083.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKDKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(14907..17312)
                     /locus_tag="POB92_000561"
     CDS             complement(14907..17312)
                     /locus_tag="POB92_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6578084.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(17378..18109)
                     /locus_tag="POB92_000562"
     CDS             complement(17378..18109)
                     /locus_tag="POB92_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDI6578085.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGEEQQVGVMVVAHGNINGKFTKAHEELQICTQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18338..18865)
                     /locus_tag="POB92_000563"
     CDS             complement(18338..18865)
                     /locus_tag="POB92_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6578086.1"
                     /translation="MMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDAVKE
                     NIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQLLT
                     YVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMRKVL
                     IPERFDETILGQPPF"
     gene            complement(19109..19933)
                     /locus_tag="POB92_000564"
     CDS             complement(19109..19933)
                     /locus_tag="POB92_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6578087.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20150..20332)
                     /locus_tag="POB92_000565"
     CDS             complement(20150..20332)
                     /locus_tag="POB92_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578088.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(20416..20883)
                     /locus_tag="POB92_000566"
     CDS             complement(20416..20883)
                     /locus_tag="POB92_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDI6578089.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21069..22619)
                     /gene="adcA"
                     /locus_tag="POB92_000567"
     CDS             complement(21069..22619)
                     /gene="adcA"
                     /locus_tag="POB92_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDI6578090.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(22913..23179)
                     /locus_tag="POB92_000568"
     CDS             complement(22913..23179)
                     /locus_tag="POB92_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDI6578091.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(23179..23430)
                     /locus_tag="POB92_000569"
     CDS             complement(23179..23430)
                     /locus_tag="POB92_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6578092.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(23595..23696)
                     /locus_tag="POB92_000570"
                     /pseudo
     CDS             complement(23595..23696)
                     /locus_tag="POB92_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23703..24302)
                     /locus_tag="POB92_000571"
     CDS             complement(23703..24302)
                     /locus_tag="POB92_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI6578093.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(24321..24683)
                     /locus_tag="POB92_000572"
     CDS             complement(24321..24683)
                     /locus_tag="POB92_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDI6578094.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            24938..26188
                     /gene="fmhA"
                     /locus_tag="POB92_000573"
     CDS             24938..26188
                     /gene="fmhA"
                     /locus_tag="POB92_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDI6578095.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSTIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(26285..27016)
                     /locus_tag="POB92_000574"
     CDS             complement(26285..27016)
                     /locus_tag="POB92_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6578096.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     EAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(27013..27732)
                     /locus_tag="POB92_000575"
     CDS             complement(27013..27732)
                     /locus_tag="POB92_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI6578097.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27713..28492)
                     /locus_tag="POB92_000576"
     CDS             complement(27713..28492)
                     /locus_tag="POB92_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDI6578098.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28613..30052)
                     /gene="mdeA"
                     /locus_tag="POB92_000577"
     CDS             complement(28613..30052)
                     /gene="mdeA"
                     /locus_tag="POB92_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDI6578099.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLLSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSRQ"
     gene            30226..30330
                     /locus_tag="POB92_000578"
     CDS             30226..30330
                     /locus_tag="POB92_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDI6578100.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30484..31170)
                     /locus_tag="POB92_000579"
     CDS             complement(30484..31170)
                     /locus_tag="POB92_000579"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI6578101.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(31497..32363)
                     /locus_tag="POB92_000580"
     CDS             complement(31497..32363)
                     /locus_tag="POB92_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI6578102.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(32862..32966)
                     /locus_tag="POB92_000581"
     CDS             complement(32862..32966)
                     /locus_tag="POB92_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578103.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            33061..34374
                     /gene="sbi"
                     /locus_tag="POB92_000582"
     CDS             33061..34374
                     /gene="sbi"
                     /locus_tag="POB92_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDI6578104.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNVKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDKAVLTLLGDGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTITGLFNSFWK"
BASE COUNT        11330 a         6679 c         4926 g        12004 t
ORIGIN      
        1 tggatgtatg tagaaaacag ctatcttagc aaacagttgt gttttttatt ttatgtttct
       61 tcaaaagaaa taattaaaaa atacacaaac tatcttaagg aatatgattt aacctatact
      121 ggttacattg ttttaatggc gattgaaaat gatgaaaaac ttaacatcaa aaaattaggt
      181 gaacgtgtat tcttagattc tggaacactg acaccattac taaagaaatt agaaaagaaa
      241 gattacgttg ttcgaacacg tgaagagaaa gatgaaagaa acctacaaat ttctctaaca
      301 gaacaaggta aagcaataaa aagccctctt gctgaaattt ctgttaaggt ctttaatgaa
      361 ttcaacattt cagaacgaga agcatcagat ataattaata atttgcggaa ttttgtttct
      421 aaaaattttg actattccga caaaaaataa ctccctatca taaggtgatg ggcagaagta
      481 tgcatcgcta tgtttattca ttatctttat tgcttgtata aatctcgatg ctcagttctt
      541 ttgccctgat tccttatcag gcacaaatct acccatttat tactttttta tctccccttt
      601 tactttaact tttttaatac tactcatata atggctttgt aatacaaatt tctataatgg
      661 aagtgatgat attgaacttc aatcaattcg agaatcaaaa cttttttaac ggtaatccaa
      721 gtgatacatt taaagattta ggtaaacaag tatttaatta cttttcaaca ccttcatttg
      781 taacgaatat atatgaaaca gacgaattat attacttaga agctgaacta gcaggtgtaa
      841 ataaagaaga tattagtatc gatttcaata ataatacgct cactattcaa gctactagaa
      901 gcgcaaaata caaatctgaa caactcattt tagatgagcg taacttcgaa tcattaatgc
      961 gtcaatttga ttttgaagct gttgataagc aacatattac tgctagtttt gaaaatgggt
     1021 tattaaccat taccttgcct aaaatcaaac caagcaatga aactacttca tcaacatcta
     1081 ttccaatttc atagttaaat ttaaaatgaa tacactacta aagccttaat gtcatgctac
     1141 gaatattgcg catgatatta aggctttgta tttaactaaa tgaatgtcat gtattacata
     1201 tcaaccgttt ttcaatgagc catattcttt ttggtataaa tgacgcatgg taaataatgc
     1261 aatacatcca aataccgcta agaaaataaa tgctaaatga cttgatcctg ttaaattagc
     1321 tacgtacgtg attactagtg gtgggaagaa tcctcctaaa ccacccatca ttgatacgat
     1381 accatttgcg gatcccgctt catttaagaa gtaggatggt actaatttga agattaagcc
     1441 gttaccaata cctgcacaaa tacttattgt taaacagcct acagtgaata atgcgatatg
     1501 gtctgaaata cctaaaataa tggcaccgat aatcataaca acaaaatcaa tcatcaatac
     1561 tttaactgca ttaaatttat cacctaaaat gccacctatt ggtcttaaga atgttgccag
     1621 cgcaatgaat acacctgatc gaataccagc atctactttg tcaattccaa aatgatttac
     1681 taagtagtta ggtaagaaaa taccaaatgc tacaaaagca ccaaaagtaa tgaaatacca
     1741 ataacttaag taatataatt tgtagttttt agataatgtt ttcatttgtg ccattaatgg
     1801 cactttaatt ttacgttctt gtgtatcacc aaatataaac ataattaatg caaataaagc
     1861 gataataatt aagtaacttc taacagttgt ttgccaacca ataatacccg caattggcgg
     1921 tgctaaaaat gaagaaactg ctgtaccgat atttcccata ccataaatac catttgctag
     1981 acctactttt tctttaggga aatatttagg aactgatgta acaccaactg agaaaattgc
     2041 accacctaca ccaaggaaaa atcctgacgc cattaacata cccggtgttt gtgcttggct
     2101 taagaaaaat atcgggaata acaatacgat aaaactagta aagaataccc atttagcgcc
     2161 aacaatgttt gttagataac caaatggcac acggagcacc gatcccaaaa taactggtat
     2221 cgctaaaatg attgatattt gaccttcagt aacattgaca tcttgtttaa taaagggcat
     2281 taatggcgca attatactcc aagccataaa cccaaccact aaacttaatg tctgtaatgt
     2341 aagttgaaag ccaccttttg ttttgtacat acgtttcacc ttcaccaaaa tcatttattt
     2401 tttaatagat atcaatactc tgcctatata ttagaggtca atgttattta aaaatgcttt
     2461 catttatatg aaatcattca atatagacaa agtattgact acatatatat tttattatga
     2521 ttgtgaaaaa gtgaacataa ggattttccc ttatttatga agtgatttac cctatacaat
     2581 gtccctttaa gatgctttta gacacttatt cgccatcatt ttaatcatat tatttaggaa
     2641 cactcacata cttttcttat ttttttcaat tagttacaat tactgcttga aaatcacgtt
     2701 acgttatcct ttatgctgac aacataagga ggtttatcat gtataaaatc aatattatta
     2761 gatcagattc cgtctataag aatatactca aggcaccaat aaacgaccgt gattcaattt
     2821 tcactaaaga aatattagta ccttttaaaa agaaatttga ggtacaacat atgcctattt
     2881 ataatgatga caaacaaaca atgagtgcga ttcaattttt agatgcattt caaaagtctc
     2941 ccaaagaact ggttgaaact gaccaaatgt caatacaata cctgaataat gatttctgga
     3001 gtaattgcga aaaatattta aaagttgcta tagatcaatt ttcaaattat agtatctcat
     3061 ttcaagtttc taactatcat ttcacagtat tacttggaga tagccaaaaa ccacttatgt
     3121 atctaaataa aaatcgcggt ggtgatggtg gcataccagg ttatattatg atttatttag
     3181 tgccgagtac aagtacaatt aattctatga aaagcttaat tgcacatgaa gtaaatcata
     3241 atatgcgcta tcaatattat tgattgggat ggcggaagtt tgattgaatt gattattgca
     3301 gaaggattag ctgaaaacta tgtagaatca ttgtatggta aagcgcatat tggaccgtgg
     3361 gtaacaaata ctaattggag tcgtgataac gtaaaaatta aaaatactat ttataatcat
     3421 ttacatttaa agcatatatt tgaatcgatg ccttatctct atggtgatga tattaataaa
     3481 cttcaaggta ggcctatcgt tggcttatct catgctgccg ggtatgcatg tggctatcac
     3541 ttggtaaaat actttttaca aaaaacaaac atacctattg aagttgccac aacacttcca
     3601 gcacaaaaaa taataaatga ggtgactgaa ttttggcata cacatacact ttaaaagata
     3661 ttattgaaat tacaggtgta actaaaagaa ctttacatta ttacgatgaa ataggattat
     3721 tagttccaga taaaaatgat aaaaattatc gcgtttataa acagcaagac ttagaaaaat
     3781 tacaaaagat tttaatactc aagtcttttg attttgatat cgctaaaata aaacaataca
     3841 tttcatatga taatgaacaa ttgcgaaagt tattatcaga gcaagtaagc aagttagata
     3901 aaaagatttc agacttacaa ttaattagac gctctgtatg tgaatttatt aacggacact
     3961 ctctaataga taccagcatt ttaaataaga cactacagtc acaatatgat aaagaagcat
     4021 ctataaaata tggtcatacg aaagcatatc aatcatttat tagacgtaaa gacagcttac
     4081 aatcgcagga tatcagacat aaattgacaa ctatcttcaa taaatttaat catatgtctt
     4141 tgagtcatta tccaatccaa gattgtagtg atctcgtatt tgagtggaag gcatttatga
     4201 acactatcgc tgattttgat gatgaaacat tatgctgtat tgctaaaaca tatgaagatg
     4261 atacgcgttt caaagattac tttaattcat atgataatca aaatttagca tcatacattt
     4321 cagaagctgt taattatttt ttgagcaatg tgaataagag cgacaatttt taatcctcat
     4381 acagatgcta caacactgaa tacaccaact aaaataattg ttcaaagtag aaaactaatc
     4441 atttattctc taaatcaata actatattaa aagttatacc tttgcaaagc gaattagtaa
     4501 aggttaccga aaggagaaag gattaggttc cattcgattt ataaaaaaat atatttcgaa
     4561 acatacaaaa gcgccagaag ataatcattt cctctcgcgc ttttatactg aattatatta
     4621 atttcaaact ctaaaactct aataatttct ttttcaatgc atattcaact agctcaggtt
     4681 tactctttaa gccaagcttt gtcataatat gcgtcttatg tgcttctact gttttcacag
     4741 atacaaataa tttctctgca atttctttat tcccgtaacc tttggcaata agtggcaata
     4801 tttctagttc tcgttttgat aagattttaa aaggatctgt tgtgtttgca gtgtcctgat
     4861 ttgaattaga tacaaattca ttcactaaag atgttgtaag tttcatatct acatatgttt
     4921 cacctttata tacagttcga atagctaaca ataattgttc atcaggtgca tttttcaata
     4981 tgtaaccttt cgcaccatta cgcaacacat ggaacaaata ctcctcatca tcaaacatgg
     5041 ttaatattaa aattttagtt tctggaaaac tgtcagcaat tttactcgta gcgataagac
     5101 ctgactcacc tggtggcata cttaaatcca ttagtaacac atcaggttta tattccatta
     5161 ctttttggta agcttcgacg ccatctgcag ccgttgcaac aacttccata tcattttgat
     5221 aatttaaaat catagagaac cccgtacgga caacagcgtg atcatcggca atgactattt
     5281 tcaattttat tcccccaatg tatgtttcaa attggaatgt tcaatgtaac attggtaccc
     5341 tcaccaattt tcgtttcaat attgacgcta ccgctgacta actcagctcg ctcattcatt
     5401 ccatataaac cgagtccaga acctttaggc ttagaacttg gatcaaaacc atttcccgca
     5461 tctatcactt ctgctaccaa atggcgccca gtttgacgga tacctacatt tatttcattt
     5521 acatcagcgt atttcaacgc atttagaata gcttcttgta ctactcgata aacaaccgtt
     5581 tcaatatcac tatcaaagcg ggtattttta atatttgatg tatatatgat ttttattcca
     5641 taattttctt caaactgttt aaaatatgat ttaaaagctg cttcaaggcc tagatcatcc
     5701 aaagaagcgg gtcttaattc aaccgacata ttacgtatat catcaattaa tttagcgaca
     5761 atatattcaa tattttctgc gtcttccaaa agcttagttg tatcttcttg atattttaat
     5821 aatctcaatt gaacatctac attgagcatt tcttgaatca cactatcatg taactctcta
     5881 gaaattcgct ttctttcatt ttcttgggct gagattgttt tacgcatcat acgttgttga
     5941 tgtaatttct cttgctgttc aatttgtgat gaaacatttt gaagcgtaaa tgcatgaatt
     6001 cccctgtctt gatcaatcaa ctgatatgtt gctgtaaatg gcatcacttt ttgatctttc
     6061 gtcttcataa atacttggaa attcgtagct tgtacttgca tcgattctaa gaaacagtct
     6121 ttacatgatt gtacatcata ggcatttgta tacccttcac atctgtgaca aatcgcattc
     6181 gccacagcac tgtaattatc ttcttcagat aatatatctt tagcagcatc attcattgca
     6241 ataattttgc cgttatcatc agcaaaaact atcttttcga ttgaatgctc ataatatttt
     6301 ttcaataaag tatctaactg tatactgtcc tcattaatca tgacttacac cctaattcat
     6361 ctcattattt atcatcattg aaaataccaa acttacgttg aatatcatca ttatcaaata
     6421 tttttggtaa aggacgacca tctctttgac caaataatag tacgccatac acttgattct
     6481 tataccaaag cggcactgct aaaactgctg ttaatgattc gctcaataaa attggatagt
     6541 caatcttttc ttcaggccct aaagctaaac caacattggc tattaccata cgctttcctg
     6601 ttttcataac agttccagct aatccacgac cttttcttaa aataatcaat ttaaatcgat
     6661 tatttttatt acctgaaaca tagtgccatt ttattggaga tgatggtttg ttagattcat
     6721 agaaagcgat tgccgcaaaa tcataaccct cttctttgcg tattttatct aatgtctctt
     6781 gaaatctacg atcttcaatt attgcttctg gtgtcaaatc ctttcacctc ttatgcttac
     6841 actttattct tacggtaaat aatatatctg cgatttatat atgtcaaagg tacactccaa
     6901 acatgcacca aacgtgtaaa tggccaacaa gccataatag tgaaacctaa caatatatgc
     6961 attttaaatg caatcggcac accactcatc aatgacgcat ctggttttaa cataaataat
     7021 tgtctaaacc aaattgataa tgaagttctg tagttaaaat ctggatgttg tatattggtt
     7081 actaatgttg cgtaacatcc cataaatacg ataagtaata ataagaaatt tacaaatata
     7141 tccgacgctg aacttaatct tcgaatactt ttcgtagtaa cacgtctcgc tgttaataaa
     7201 aacatcccta tcaaagttat tataccaaag atgctaccaa tataaacagc gcctatatga
     7261 tataaatgct cagacacacc cactgcatcc atccatgatt tcggtattaa caatccaact
     7321 acgtgtccaa aaaacactgg aataatacct aagtgaaata ataaacttcc ccacatcaat
     7381 ctttttcttt ctattagttc actagattta gctgtccaag aaaatttatc ataacgataa
     7441 cgtgcaatat gacctgcgac aaagacaact aaacataaat acggaaatat aacccataaa
     7501 aactgattaa gcatgatgtt tcactccttt tggtgatgtc aaacataatt tcaatgtttt
     7561 tctaagtgct tgaatcacat aggcatatgg attgttatct tcaccaagtg cattcgccat
     7621 cacatatgtt ccatcctcaa taatcataat gattaattga atattctctt cagctcttgg
     7681 atcatttcgc cattctgcca cttgcaaaaa ttgaagcatc aacggtagat aatcagaaag
     7741 ttcattatct accatttcta gtccaaacat ttcatataat acctttaatt tagctaacat
     7801 ttgcccacgt tctttttgcg tatcaaattt gttatacgtc atatataatg gtgctttttt
     7861 cgtaaaatca aatgtatctg tataaatcgc tttgatttct gataatgaaa atgtcatcat
     7921 tacatttcta aatgctacta aatcttcata tgctggatga tctgtcgaaa ttgtttcttc
     7981 aaaagttttt ggatgaaaag ttaatttttc tggaaagcat aactgttgtg ccatatatcc
     8041 aaaactttct tgatattttt taaaattatc gaaattaatc acggaaaatc cctccataga
     8101 aattctcatt ataaatttct tgaccagttt tccctgaacc tactgcaacg ccacagcctt
     8161 cacagttatc tccaaaatgc tcgccgccgt aattgtatcc tgtactacct tgtgcgtgat
     8221 acgtatctaa ataggtttct ttgtgtgatg ttggaataac aaatcgatct tcatatttgg
     8281 ctagtcctaa taaacgatac atgtctttag tttggcgctc ggttatacct aatcgctcta
     8341 atcgagacgt gtcaaatggc tgttgagtaa cttgagatct catataactt ctcatcattg
     8401 ccatacgttg tagggctcct tttactggct ctgtatctcc tgcagtgaaa atattagcta
     8461 agtattcaat aggtaaacgc atttcttcaa tggctgggaa aatcgcatct ggattttgag
     8521 ttgtattttt accttcaaaa tagctcataa ttgggctaag tggtgggcaa taccaaacca
     8581 tcggcatcgt tctaaattca ggatgtaacg gaaatgcaag tttatattca attgctaact
     8641 tataaattgg agagttttgt gcagcttcaa tccaatcata accaatacca tctttttcag
     8701 cttgagcaat gacttcttcg tcaaatgggt ttaagaatat atctaattgt ttttcgtata
     8761 aatctttctc gtctactgct gaagccgctt catgaactcg atctgcatca tataataaaa
     8821 cacctaagta acgcatacgt cctgtacaag tttcagagca taccgtaggc atacccgcct
     8881 cgattctcgg gaaacagaaa gtacattttt cagctttgtt cgttttccaa ttgaagtaaa
     8941 ctttcttata tggacaacct gtcatacagt aacgccatcc acgacatgcg tcttggtcaa
     9001 ctaatacaat gccatcttca tcacgtttat acatagcacc tgaaggacac gatgcaacgc
     9061 aacttggatt caagcaatgt tcacataaac gtggtaaata catcataaaa gtttcgtcaa
     9121 attggaattt aatatcttct tctatttttt ggatgttagg atcttttgga cctgtaacat
     9181 gaccacctgc taagtcatct tcccagttag gtccccattc aatttcaatg ttatcccctg
     9241 taatttctga atacgctcta gcaactggcg aatgcttccc tgatttcgca gttgttaaat
     9301 gttcataatt atagttccat ggctcataat aatctttaat taatggcata tctgggttat
     9361 aaaaaatttt acctaaagca atttttgaaa ttctacttcc agattttaat tcaagtttcc
     9421 ctttacgatt tagtacccaa ccacctttgt agtgttcttg gtcttcccaa cgtttcggat
     9481 accctacacc tggcttcgtt tctacgttgt tgaaccacat gtactcagca cctggacgat
     9541 ttgtccaagt gtttttacat gtcacactac acgtatggca tcctatgcat ttatctaaat
     9601 ttaataccat cgcaacttgc gctttaatct tcaagccaat taacctcctt catctttcta
     9661 actgctacat ataaatccct ttggttccca attggtccat aataattaaa gtgataacta
     9721 atttgtgcgt atcctccgac tagttgtgtt ggtttcaaat ggattctagt cggcgcgttg
     9781 tgtgaaccac cacgtgtatc tgtaatttct gacccaggcg tttgaatatg tttatcttgt
     9841 gcatgataca taaacattgt acctttaggc atacgatgcg aaataactgc tcttgccgtt
     9901 acaacaccat tacggttata cacttctagc caatcattat cttggatatc gtgtttttca
     9961 gcatcttcat ttgatatcca aaccgttgga ccacctctaa atagtgtcaa catatgctta
    10021 ttatcttgat acattgagtg tatattccat tttccatgag gcgttaaata acgcagtacc
    10081 aaagcatctg taccaccttt aattttctta tctctattcc caaataccat tggcggcaat
    10141 gtcggtttat atactggtaa gctctctcca aattgttgga aaacttcgtg atccacataa
    10201 taactttgac gtcctgttaa tgttctaaaa ggtactagac gttctatatt cgttgtaaat
    10261 ggtgaatatc gtcgaccttg tttatttgaa cctgggaata ctgctgtcgg tattacttct
    10321 cgtggttgtg aagttatatt taaaaacgaa attttctcag cagcgcgttc gctagaaata
    10381 tcttttaacg gcattccagt ttgttcttcg agatcttcat atgatttttg tgataattta
    10441 ccattcgtag cagatgaaat acttagtatt gcatcagcta cattacgtgc tgtatcaata
    10501 cgtggacgat tcgctctcac agaatcatca tttgtatcac tccacgtacc taacatactt
    10561 tttaattctt catattgttc actgacaccg aaacttacac catgtgctcc aactttccct
    10621 ttttcaagta caggaccaag cgtgacatat ttgtcgtaaa ttttagtgta gtcgcgttct
    10681 acaattgcaa agttaggcat tgtacgtcca ggtaccgctt caatttcacc cttcgaccaa
    10741 tctttcacta cgccgtatgg tgttgaaatt tcttgctttg tatcatgact aagcggagtt
    10801 gtcacaacat ctttaaacgt tccaggtaaa tagtcttttg ccatttctga aaatgctttt
    10861 gccaacgttt tataaatatc ccagtctgaa cgcgattccc ataatggatc aatagctgga
    10921 ttaaaaggat gtacatatgg atgcatatct gtagatgaca aatcatgctt ctcataccaa
    10981 gtcgctgctg gcaaaacaat gtcagaatat aaaggtgttg ctgtcattct gaaatctaaa
    11041 gaaacgacta aatctaattt ccctgttgtt tcctcacgcc acgtaatttc ttctggcttt
    11101 tcattttcat ttggtgtagc taataaccct gattttgtgc caagtaaatg cttcataaag
    11161 tattcttgac cttttgcaga acttgagatt agatttgagc gccatataaa cagtgatttc
    11221 ggatgattct ttttcaaatc cggatcttct atcgcaaatt gcgtttgttt tgacttaact
    11281 tcatttatcg ctcgttttaa aattgcttca tttgaatcta taccttcatc tttagcttct
    11341 tctgcaaaca acaaactatt tttattaaat tgtggatatg atggtaacca accaagtcta
    11401 gctgctaaaa cattataatc agctggatgt tgatgcttta actcctctgt tttagctaat
    11461 ggagatttta aacgatctac atttgactct tcatatttcc attggtctgt tgcaaaatag
    11521 aaccaacttg ttccgttttg caaacgtggt ggtccttgcc agtctttcgc aaatgcgaca
    11581 gtactccatc cttcaatcgg acgacatttt tcttgtccca catagtgagc ccaaccgcca
    11641 ccattcacac cttgacagcc acataacata actaagttta agattgaacg ataaatcgta
    11701 tctgagttaa accaatggtt aatacccgca cccatgataa tcattgaacg cccttcagta
    11761 tcgatagcgt tttgcgcaaa ttctttcgct acttgaatga caacactttg ttttacgcct
    11821 gaaatggctt cttgccaagc aggtgtatat tttgattctg catcgtcgta tccttttgat
    11881 tctaatttat gatcaaaacg acgcacgcca tattgacttg ccattaagtc aaaaattgta
    11941 gcaatacgga ctttgtcacc atttgctaaa gtgacttgtc gagttggaat tggacgattg
    12001 aatatcccat ctccatcact atcaaagtat gggaattgaa ttgtttctaa ttcgtatcca
    12061 ccttctgcca ttgataatgt agggttaatt ttagaaccat cttctgtttc tagttttaag
    12121 ttccacttct taccttcttc ccaacgttga cccattgtgc cattaggtac tactaaacta
    12181 tcgctgattg catcatgaat aactggcttc cattcgcctt gctctgttgt ttgacctaag
    12241 tcactcgctc ttaaaaatcg acccgcttta tatccatttt catcttcatc cagcatgata
    12301 agaaacggca tatctgtata ttgtttagcg taatttataa agcgttcatt aggttgatta
    12361 acataatgtt cttgtaaaat aacatgcgtc attccttgtg caattgcagc atctgaacca
    12421 ggattcggtg ctagccagtt atctgcaaat ttcacatttt ctgcgtaatc tggtgctact
    12481 gaaatgactt ttgtaccttt atagcggact tcagtcataa aatgtgcatc cggagtacgt
    12541 gttaaaggta cattagaccc ccacataata atgtatgatg cgttatacca gtcgcttgat
    12601 tcaggcacat ctgtttgctc tccccaaatt tgtggagagg caggtggtaa atctgcatac
    12661 cagtcataaa aactaagcat ttcaccacca agcaaattga tgaatcgagc acctgctgca
    12721 taactaatca ttgacatcgc tggaataggt gtaaatcctg cgattcgatc tggaccatat
    12781 ttttttattg tatacagtaa ttgtgctgcg attatctctg taacgtcttt ccaatttgaa
    12841 cgcacgtgcc ctcccatacc tcgggcttgc ttatattgtt tggctttgtc ttcattttca
    12901 acaatagacg cccatgcagc aacgcgatta ccattgtttt cttctaatgc ttcagtccat
    12961 aaatcccaga gttttccacg aatatatgga tatttgattc gaagcggact gtattcatac
    13021 caagagaatg acgcacctcg tggacatcct ctcggttcgt attcaggcat atccggacca
    13081 caacttggat agtcagtttg ttgattttcc caggtaatca caccattttt cacaaatact
    13141 ttccaagaac atgagcctgt acagttaaca ccatgtgttg ttcttacttc tttatcgtgg
    13201 ctccaacgtt ctctgtacat tttttcccat tctctacttt tactttctag gatcgaccaa
    13261 ttcccattaa atttttctgt tggcttaaag aaattcaatc caaattttcc catatttata
    13321 tcctcctacg tataaaaata cgatgtgtag atgtcgtgtt tttaaatact ttaaaatgcc
    13381 caagactatt gctttaatta gattgtacat tttttcacaa acataaaata ttagggaatc
    13441 acctaattac ttaaggaatt tccctatcaa taacgggatt tcattgaaat aatacacaat
    13501 catgtatggt cgtgcttatt gccaatctaa atcgttcaaa tttggcacaa cgacaaataa
    13561 ggcttcaaca cgaatatatt ctctcggttg aaaccttact tattcattta tttttttaaa
    13621 ttagtgacat aacactgtat tagcatctgc acgatcggtt gaaatatatg ttacattttc
    13681 ttgctgctta ataaatgcat catagtaatc atattgcgac gaatgatatg tgccattcga
    13741 tgtattattt gggtttagca aacagccata accttcgtca tataaatgtt cacagagcat
    13801 aagggcgtca tgtttagaac cacttactac ataaaattgc tgagtcggat tagaagttgt
    13861 tgagacattt tcagtataac caacaacttc cccaataata catatacctg gtttcgcctc
    13921 aattgaatag tgttgcaatt ttgaaataat attacttaaa cgccccttaa caacaaactc
    13981 gttaaaacac gatgcttgaa agacaatcgc tatcgggtaa tcaatatctg tgtattgttg
    14041 tatctgtgtg ataattttcc ctaaacgttt tacccccata taaattgcta acgtgccacc
    14101 attcactaag gaattgacat ccacttcatt ttcttctgaa tctttaaagt gacctgtaga
    14161 aaatgtcaca cttttagcaa ctgtacgcat tgtcaaacct gtctgcatag tagcaactgc
    14221 tgcgctcgct gatgtcaccc ctggtacaat ttcaaacgca atatgatgtt catttagtgt
    14281 gtcgacttct tcttgcacac gaccaaatat cgctggatcg ccacctttaa gtctaacaac
    14341 cttgttatat cgacgcgctg cttccacgat acagtcattt attttttctt gctgaatatg
    14401 ttttgcatac ggctttttac caacatcgat aatttcagta gtcaaattcg catattgtaa
    14461 aattaacgga ttcactaatc gatcatatag aatgacatcc gcttcacgta ttaaacgctc
    14521 agccttttta gtcaaataat tcggattacc tgggcccgca cctatcaagt aaaccttgcc
    14581 atattcctct acagacatat atatacgttc ccgtctgtaa cttctacctc ataaacatct
    14641 acacaacctt catcaggttc ttgaacaata cctgtattta aatcaatttt ttgatcgtgg
    14701 agcgggcaaa atacatattc cccactcact gtcccttcag acaatggtcc ttgtttgtgt
    14761 ggacagatat tgtgaatcgc atgaattttg ccactttctg ttaaaaacaa tcctacctct
    14821 ttgtctttga caataacctt ttttccaatt aggggtgtta attcatctat agttgtcact
    14881 ttaatttttt cttttgtttc catgtattac accttctcca cttcaaaaat tttacgtgct
    14941 tgcgcattgc tagttattgc ttcccaaggt tcagcttcga ctgctttttt agcatccata
    15001 atgcgttcaa atagttcatt ttgtctttct gggtcaagta agacttcttt tacattttca
    15061 aatccaagtc ttcttaacca tggcgctgtt ctttcagcat atatacctgt ttcgcgataa
    15121 tattgcatca aagcaccaca taatttgatt acttcatctt ctgtttctac agttgttaaa
    15181 aattcagctt tttcaacttc tgtaccacca ttaccaccga tatagatttg gaatccattt
    15241 tcaactgaga taataccaaa atctttaaca cctgattcaa cacaacttct tgggcagcct
    15301 gatacaccca ttttgaattt atgaggtgta tcgatgtatt caaatgtttt ttctaaacga
    15361 atgccaagtc gtgtcgtgta ttgcgtacca aatcgacaaa actctttacc aacacaactt
    15421 ttaactgagc gagttttctt accataagct gatgctgaac gcatacctag gtcttcccat
    15481 atatttggta attcttcttt tttaactcca tacaaaccaa cacgttgcga acctgtcact
    15541 ttaactagtg gcacatgata tttcttagcc acttctccta gacgaatcag ttggtctgca
    15601 tctgtaacac ccccacgcat ttgaggtata acagaaaatg taccatcatt ttgaatattc
    15661 gcatggtaac gttcgttagc aaatcttgat tctctttcat cttcatgatc atgtggataa
    15721 accatattca aataatagtt aatcgcaggt cgacattttg gacatccacc tttgttttta
    15781 aagtttaaaa catgtcgaac ttctttagat gtttttaaac ctttcgctct tatttgcgtt
    15841 acaatttgat cgcgtgtcaa atcagtgcaa ccacatatac cagcaggttt tgcggcaaca
    15901 aagtcatctc ctaaagtgtg ctgcaatatt tgagcaattt gcggtttaca tttaccacat
    15961 gaattccccg cttttgtttt agccgttact tcttcaactg ttgtaaagcc attttccgta
    16021 atcgcattta ctatagtacc tttatcaaca ccattacaac cacaaattgt ttcatcatca
    16081 gccatatcag caattgatag cgatgcctct tctccacctt tagtaagcaa tgatacaagt
    16141 gtgtaatctt cagtggattc accttttttc atcatgttat aaaagcgtga accatcatcg
    16201 atatcaccat atagtactgc accaactaca ttaccgtctt ttaaaaagat ttttttatag
    16261 ttattatcaa cactattaaa tatttcaata cctttaattt ctgcattttc tacaatttga
    16321 ccagcactat acaagtcaca cccagaaact tttaatgacg taaatgttgt tgatcccttg
    16381 tatccgttcg tttctttatt tgttaaatga tcagctaata ctttaccttg ttcatatagt
    16441 ggtgcaacga gtccataaac tttgccgtta tgttctgcac attcaccaac tgcatataca
    16501 ttgctatcac ttgtttgcat cacatcattg acaacaatac cacgattaac atctagacct
    16561 gattctttgg ctacttctgt gtatggtcgt atacctactg ccataacaac taagtctgcc
    16621 ggaatctcgc gtccatcagc caatttaaca ccctcaacat catcttctcc taagatttca
    16681 gttgtgttgg cttgcatttc aaacttcata ccttgctttt ctagatctgc tttaagcata
    16741 tttccagctt tacggtctag ctgcatttcc atcaaccatt cagctaaatg taacaccgtt
    16801 acttccatac cttgatctaa taaaccacgt gcacactcta aacctagtaa gcctccacca
    16861 attacaattg ctttcttttt agtcttcgca atgttcatca tttgttcagt atcatcaatc
    16921 gttctccaac caattacact cggtaatgtc gaacctggaa ctggaatgac gaatgcttta
    16981 gaacctgtcg caaaaatcaa tttatcgtat gatacttcaa taccatttgc agtagtaact
    17041 gattgatttg ctctatctac ttcaattaca ggatcatttg taattaactc gataccatgt
    17101 tcctcatacc actcatatgg attcataatt gtttcttcaa ctgtcatttt attttgtaaa
    17161 atatttgaaa gcatgatgcg gttatagttt ggataaggtt ctttacctat taccgtaata
    17221 tcatataaat cgttggcgcg ctctaatatt tcttcgattg ttcgaatgcc cgccataccg
    17281 ttaccaatca ttactagttt ttgctttgcc ataaaatatg cccctttact ccataatatt
    17341 tatttcaaaa aaaggtatta atttttcgtt agtgctttta tattttcatc ggaatcatta
    17401 agctttctaa tctatcgtta atgatttgct ttaaaattgg gtcgaagtta attgaaggtg
    17461 tgatgtgtat atctgtatta ataaccatgt cattcatttg ctgcttcact ttgttaacaa
    17521 gccttccgtc gtataaaaat aatggtacga caatcaattt ttgataccgt ttcgagatgc
    17581 tttctaaatc atgtgtaaaa ctaatctctc catatagcgt tctcgcatat gtcggcttgc
    17641 taatttgcaa attttgagtg catatttgta actcttcgtg tgccttagta aactttccat
    17701 taatattgcc gtgtgcaaca accataactc caacttgttg ttcttcacct gctaatgcgt
    17761 cacaaatacg ttgttcaatt aatcgtctca ttaaaggatg tgtgccaagt ggctcgctta
    17821 cttctacctt tatgtctgga taccgtcgtt tcatttcatg aacgatattc ggtatatcct
    17881 tgagataatg cattgcacta aagattagca atggtacaat tttaaaatgg tcaaccccac
    17941 tttgaatcaa cgtcgtcatt accgtctcta aatcctgatg ctcactttct aaaaacgcaa
    18001 tatcatagtg atgtatatca tcttttacta attcagaaat aaatgcttct aacgcttgat
    18061 tctgtcgtcc gtgcctcatg ccatgtgcaa caatgatatt cccattcaca tttaccaacc
    18121 ctttcacacg tattgtatac caaatcattt tgtttttgtg aaaagaatca cattataatg
    18181 taaaatcagg gaattccctg atgcctgtag tcatgcatat tccttataca ttttcccttt
    18241 ttgttaaatc aaaaaagcga ccgatatatg aatccctact caacatttat ttgagcaagc
    18301 attaatatat cggtcgcttg tagtgtatat tattatctta aaatggtggt tggcctaata
    18361 ttgtttcgtc aaagcgctcg ggtatcaata ctttgcgcat gatcacacct aaatcgccat
    18421 catcattttc atgttcgctg tatatttcat aacctctttt ttcataaatt ttaagtaacc
    18481 acggatgcaa tcttgcagat gtacctaaag taactgccgc tgactttaac gtatctcgca
    18541 aaaatgcttc ttcaacataa gtaagtaatt ggctaccata gcctttccct tcatactcag
    18601 gatttgtcgc aaaccaccag acaaaaggat agcccgaaat acttttcaca cttccccaag
    18661 gatatctaac cgtaatcgta gatataattt catcatcaat tgtcatgaca aatgtagtat
    18721 ttttatctat attttcttta acagcatcta aattagcatt aactgaaggc caatcaatac
    18781 ctagttctct tagaggcgta aatgcttcat gcatgagttg ttgcaatttt tctgcatctt
    18841 gttcacttgc gagtcgaatc atcatttttt gtcatattaa tccccactct tttttaaatg
    18901 atttaaccat attttatttt taaaataaat atccatcaaa gtgtatcaat aaatttatca
    18961 catgttagaa agtatgcttc atctgaatac accaatactc tcataaaact tattaaaaat
    19021 tactctctca acgtaaaaaa accattcaaa ttcatgaatg gtttggaaga atgattcatt
    19081 gttacgctat ttaatcacta catcttaatt attgttgctc taaacgatta cgcttaccat
    19141 ttaagaaagc ataaacgaga cctacaaaaa taccgccacc gacaaagtta cctaagaaag
    19201 caaaaacgat attttttaaa acgtgtaacc atgaaactgc atcaaggtta aagaatacca
    19261 tacctgcata tagacctgca ttgaacacaa cgtgctcata tcccatgtat acaaagacca
    19321 cgacaccaca agctataaag aatgcctttg ttaagccgcc tttgaattgc atagagatga
    19381 aaataccaat attaataaag aagttacaga aaataccttt tgtaaaaata ttcaaccatg
    19441 ttgaatcaac agtctttttc tgaactaaag ctgttaaagc ttgtgtcatt tcaggtgtca
    19501 tgacatgcgc aaatttcatt aagaaaaata acacaaaccc acctaaaata tttcctaaga
    19561 aacaatataa taaaatccaa gtcatcttct taacagaaac gactttataa taccagccta
    19621 ctgtaaagta catgaagtta cttgttaata attcagagtt agttaataca actaaaatca
    19681 aacctaaact aaaagcaatg gctcccatta aattgataag tccatctacg tgagtactcg
    19741 caaactgcgt ctttattccg aacataaata cagtaacgat tgagagtaaa aatccggcca
    19801 tcattgcttt aagcatatat cggccaggtg tttgctgtac cattacttgc ttcatttgta
    19861 ctgaagatga tattgaatca acaattgcgc ctgttgaata tgtatcttca actgttttct
    19921 tattttctat cacagtactt cccccattta ctttgatcaa ataaaattta ctttctaatt
    19981 atattagtaa atcaatgtaa ttgatagttt aaatttcaga aaaatctttt tattctctca
    20041 aaaatgtagt tttctttcat aaaaacagcg agataaatcg cgtgattggc taatatcttt
    20101 atgatttaag atattacgac gctcatttta tctcgcttta tgtaaagtat tacttattct
    20161 tttttaaatt tgaaaagtaa tgtaaaccat atgcaaatac actgtaacca attgcagtac
    20221 gtaaaataaa ccttataact ttattaggta cacattttga cgttactgaa atagaagata
    20281 gtagtgttgt gaaaatatat aatattaaaa ctttgatata acgttctacc atcgaatcat
    20341 ccctcatttt tataatatta caagattatt ataaaccgaa tgaaacaaaa aagtttttac
    20401 atattttcaa caactttatt tcaataatgc atcaagttga tctaaagttt gattcatacc
    20461 ttgttcaaca cccatgttta tgacttgttg agcggcttct tttgttggaa ataccgatgt
    20521 tgatgtcact gtcgttttcg ttgtattgct cttggaaaat gacaaagtaa ttttcatgct
    20581 tggcattttt gtatcttttg caccttgagg tgtcgcaaat gagtcaatat attcaattaa
    20641 atatggacgc ttaaccgttt tatattctgc taatgtatag ctggtcattg taggtgtttt
    20701 aatcgcataa aatgcatcac cgcctgaaac agcattaaag cgaaacactt tggtactagc
    20761 gtcttttgga tgaaaccatt tttcaaataa tgcttttgtc gtataggcat caaacacctt
    20821 ttcaatcggt gcttctatcg ttcttgaaaa aataatttta ttatcttcaa ctttaatcgt
    20881 catcttccca ctccctatct tttatattta ctcatcttaa catgcattga gtaacatatg
    20941 ttaccaaatc atttgttata aactatagta atattaatca ttccctctgt tgaaattgat
    21001 aactacgaaa taaaaaacac actctattca gctattaaca gttgagtaga gtgtgcaaac
    21061 ctgttgaatt aatgcgctaa catttcttct ttgatttggt ctttatttaa ttttgaagga
    21121 taatatgttg gccagttatc catttctttt aataacgcgt cattatcatt acccataaag
    21181 atatggaagt gttctgcctt tttaggtgca atgttgtgat cgctaaattg gatgaatttc
    21241 ggtaagtctt tatcattacc atcgaccaat ttaaacataa atctcacgcc acggttacct
    21301 ttaggatatt tcaatgtttt cttaccattg tattcatatt taccagtgtg tttcttacca
    21361 tctttagtaa atgtaatttc atttcctttt atatcaatgt tagtaatatc agttttatat
    21421 cctttgtcat aataagcttt taaatcttta gcagatttct tcggatcatt ttcagcttta
    21481 tgttccatca cttcatcaag cgtaccgtct tttaagtaag ggtaaacaga ttgccattca
    21541 ccagcataat cgcttaattc acggtcttta acttgctcat ctttaaaata cccatcagaa
    21601 attgctttgt catgtttact ttcagctttg tcgtctttca ctttaacacc actgtctaaa
    21661 gctttaccga tattttcaat attagatttc attaatgatt gatacgtaat attatctttt
    21721 ttctgttgtt ctttatttaa agactccatg ttgtagaatt ttaaaggctt cgcatctgtt
    21781 tctttacgaa ttgtttcagt cactttattc gcaacattat cttcgtaaag aatatattta
    21841 gcattgctat ctctaatttc tttaacaatt ttagttaatt ctttttgtga tggatcttca
    21901 gcattcatgt tttgaatacc tttttgaaca aaaccataac gatcagctaa gtatccaatt
    21961 gattcatgtg aaatgaatac tgcattacct tgcttatctt ttgtaacttg cttcatatcg
    22021 ttatcaattt tcttaagatc gtcgtttaat tttttgtagt ttttctcata gtcatcttta
    22081 tgttttggat cttttttcac taattcatct ttaatttctt tagcgaaagt ttgattaatt
    22141 ttaggatcta accatacgtg tggatcatat ccaccatgat gatgatgatg ttcttctttc
    22201 tcatgatcat gtccctcatg ttcatgctct tcaccatgct cgtgttgatc agttaaaagc
    22261 tttgctttat ctaatttatc ctctaaagac agttttttat ctttatcttt gatagtagat
    22321 gcaactttct tagcaaccgg atctaaatta tcccctgtat acataaacaa gtctgacttg
    22381 cttgcactta atatatcttt ttgtgttggt tcatagctat gtaaatctgt ccctgctgga
    22441 tagattgatg ataccttcac gtgttttcca ccaatttgct ctgcaaatga ttgcaatgga
    22501 taaactgtcg ttttaattgt taccttgcca tctttatctt ttccatcatc attcccacat
    22561 gcggctaatg ataaagttac ggctggtaca agaagtaaca tacctaattt ctttttcata
    22621 gtcaccctcc taaatagtaa tctttacgtt ttaaaattat atactcatgc tacttaaaat
    22681 acaagcactg tttttataaa taatataaag tcgaatcata ctttaggtat ttaccacttt
    22741 tcgttttatc taatacatga ctattgtaca aatatgatac agaaaacttt atagtattta
    22801 cgaacgtcat aatataagaa aaaagacaat ctctatcata ttgctaatag aaattgcctt
    22861 ttcatattgt cctaaaataa cttaccctat ttttatagtg tgacttttag atttaataat
    22921 ggtatttaca tgatgctatt tttatatgat tgtcatctac tgtataaacc aatctatgtt
    22981 cgtgattgat acgtctacta taatacccag tcagattcga ttttaacttt tcaggcttac
    23041 ctataccttc caatgcacca tttctgtcaa tacttttaag taactcatta attttcttca
    23101 ccattttttt atcgttctgc tgaaaatact tataatcttc aaaggcttga ggcgaaaacg
    23161 taatatttaa cctagccatt atacatctat atctttcgtt atagttttcc cacgttctag
    23221 atctgcaatt gattgagcta agtgttcagc attatttggg ttctgttgga ggtaaagtgt
    23281 ttccatcatg gagttataat ctgattctga catgattact acgtttttat catcagtaga
    23341 tgttacagtt accatatcac tatcatcatt tacttttgtc ataagtgcct ttaaattctg
    23401 tcgagcgtat gaataatttt taataatcat atttatcgcc tccttttaac acacctcaat
    23461 tgtacaaata tctgtacaat aaaacaagcg acacagaaaa aaactactaa tcaagatttc
    23521 gcacggggaa gaatcaatac ctatcctgta ctgattccct actaaacaat gtttacatga
    23581 taatttctac ctatttagac ttcatacatt aaaaagacaa tctctatcat atcgctaata
    23641 gaaattgcct tttcatattg tcctaataaa ttgatatcac ttacttatct caacaattaa
    23701 gactatttta ttttatcttt taataacttc tcataacttt cataatcata tggatcttca
    23761 actttctcgc cattaataaa tgcagttggt gtcgttttta tatgattatc tttcgctatt
    23821 tttttatctt tctctgcagc tttccaagac ttgctatctt ttgtcttgta atcttttata
    23881 attttatttt ccgtctcttt atctaaatgc agttgtttaa tatgtttatc taatagttct
    23941 tttgttaacc attctttatt ttcatcttgc tgggcagcaa ataattgctt ttgaaaatct
    24001 aaaaatgatt taggtgcata catcaatact gcatgactcg cacgcgaacc aacaattgag
    24061 tctttaccta agaaagctaa attgacaaat tggtattcca ctttgtgatt atctatataa
    24121 tttttacgca actttggcat gactttttca tctaattctt tacaataagg acatttatag
    24181 tcgccatata tgacaactaa tggtttgccg tttttcgagg atgtcgttgc tgattctttt
    24241 ttaccgcaag ctgttaaaat taacatgctc actataaata atgttagtaa ttttttagtc
    24301 ataataactc ctgttcatat ctagttcgtc atattttctt catgataatc cgcatcatgt
    24361 tgttgataat atcgtctcga atcaaaatct tgcttatatg atacacgttt atcactaaaa
    24421 tcatatgcat aataatgcac ttcatcatca ttactcaaag gtttataagc taaaatgata
    24481 attttatcct tatcatagac ataaatatta gcatctttgc gttcaaaatg atcaacacga
    24541 tcaccgttgc cttttttggc aagttgttct tgtttttgat tttgaatttt aactgcttca
    24601 tctatttctt tcacatattt atcattacca catcctgata taaagactag taataataat
    24661 accgtcgcca caaatcgctt caatgctccc actcctacta tcaacttcca actaactttc
    24721 aatataatta atactataca ccgtcaaaca ttaatgcgct acaactacta ccaatgcatt
    24781 taatataaat atccaaagaa ataacagtta tgtatagtca aagaaaaata tatttcattt
    24841 tctatgtgta tttgtaatca gtttttgata aaataaaaaa atagcattat taataaattc
    24901 aaaaattaca gttcaattca gagagggatg ggataaaatg aactttgtaa cgttgacttc
    24961 ggatgagttc aatgcgttta caacaaagca tttttcacat tacacacaat caactattca
    25021 ttacaatcat agagttgatt taaaaggcga tgtgcatctt gtaggggtta aagatgacaa
    25081 tggtcaagtg attgcaggat gcttattgac agaagcacgc acacttaaat ttttcaaata
    25141 tttttataca catcgcgggc cagtgatgga ttatacaaat caatcattag tagcattttt
    25201 ctttaaagca ttaacgtcat atttaaagaa acacaattgt ttatatgtcc ttgtagatcc
    25261 atatttaatt gaaaatttac gcaatgcaga cggtgaaatt gttaaatctt atgataaccg
    25321 agcatttgtt agaacaatgg ataaattagg ttataaacac caaggtttcc ctgtaggtta
    25381 tgattcaatg agccaaatcc gttggctgtc agtgttagat ttaaaagata agactgaaga
    25441 ccaactttta aaagaaatgg attatcaaac gagacgtaat attaaaaaaa catatgatat
    25501 tggtgtcaaa actaaaacgt taacgattga tgaaacgcaa acttttttcg acttattcca
    25561 tatggctgag gaaaagcatg gtttcaaatt ccgtgagtta ccatactttg aagaaatgca
    25621 aaagttatac gatgaccacg ccatgttaaa gttggcgtat attgatttaa acgagtattt
    25681 aaaaacgtta caattaaagc aacaacaatt aacagctgaa ctctcaggtg ttgaagaagc
    25741 attggaagaa agtcctaatt caaagaaaaa taaaacgaaa cgcacacagc tagaacaaca
    25801 gctaaatagc aataagcgta aaatcgacaa tacaatagaa caaattgaac aagatggtgc
    25861 agtcttaaac ttagcttctg ctttgtttat ctacaatgag catgaagttt attacttatc
    25921 tagtggttca aaccctaaat ataatgctta tatgggtgcc taccatttgc agtgggaaat
    25981 gattaagttt gctaaagcgc atcatattga tcgctataat ttttatggta ttaccggtga
    26041 cttttcagag tcatcagaag attatggagt ccaacaattc aaaaaaggtt ttaacgcaca
    26101 tgtagaagag tatatcggcg actttattaa gcctatcaaa ccattactat ataaggtaca
    26161 aacatactta aatcataaac gtcgttaaaa tatcgctcca agattatgac aacataacaa
    26221 agaaccacgc atcatgtata tggcatcatc accatactaa aacatgatac gtggttttat
    26281 ttgattattc ttcatttata acatttaaga aacgtcttaa ttcttctgtt ttcggacgat
    26341 tgaacatctc ttctggagcc tcttgttctc cgatcatgcc ttcatgaata aatacaatgt
    26401 tattagatac ttctttagca aaacgcattt catgtgtcac aatgaccatt gtcatgcctt
    26461 cattagccaa atctttaata acctttaaaa catcattcac aagttcagga tcaagtgcag
    26521 atgttggttc atcaaacaac atcactttag ggttcattgc tagtgctctt gcaatagcaa
    26581 cacgttgttg ttgaccacct gataatgcat gtggacgttg atctttgaca tgtgttaaac
    26641 caactttctc aagtaactca agtgatttcc cacgtgcctc atcctttttc aactttttaa
    26701 ctgtgataag accttccatt acattttcta atgccgtctt atgcggaaaa aggttataac
    26761 tttgaaatac cataccagac tgtttacgaa cttctatttg tgatttttta tctttagatg
    26821 tatatgtttt gccgttaaca taaactgtac cttcagttgg aatttctaat gcattaatca
    26881 tacgtaacaa tgttgtttta cctgaaccag atcgaccgat taaggttaca acctcacctt
    26941 gttcaacaga taaatcaata cctttgatga cttcaacatc attgaatgat ttatggatat
    27001 tgttcaattg aatcatgagc gataccctct ttcaatataa gattcataga taccttggat
    27061 aattgaaata ataaagcata ccacccagta cattagcgca actaacacat aaattgttaa
    27121 atattcatat gttgtagacg caacttcttg agcttttcta aacatttcag ccactaaaat
    27181 aaatcctaat aatgatgtat ctttgattaa acttaaaaat gtattaccta gtgcaggaat
    27241 cgacactcga attgcctgcg gtaaaatgat acgttgtatc gtttgaccat acgtcatacc
    27301 gatggaatat gcagcttctg tttgtccttt cggtatagaa ataataccgc cacgaataat
    27361 ttccgaagca tacgcaccta catttaatga taaaccaata atagcagcca ctacaggtgc
    27421 caatgtccat tggttgtcag cgtcatttgt taataatcta cctaattctg gaataccata
    27481 aaatatgata aatagttgta ctatcatagg tgttcctcga ataatagata catagacacg
    27541 tgaaatactt cttaaaattt tactcgttga aattcgcatt aatgctgtaa ataatgcaat
    27601 aatcaatcct aaaacaaatg taactaatgt aataggaatt gaatatttga ccaaaccttc
    27661 tagcataggt ccgaaagctt gttttgcagc atctagcgca tgttgttgtt cgctatttag
    27721 atttagaaac atcttgacca aaccatttct tacctatttt agctaattca ccgttttcct
    27781 cgattttttt caagccatca ttgaattttt gaactgtttc atcatctgct tttttagaaa
    27841 atgcaaatgc agatctactt tgttcagcat tacctttgat tgctttgatc ttagcattag
    27901 gtttttgttt tttataatcc aagtatgaca gactatcatt aaatgtacca tcaacacgct
    27961 tagacaataa taaatccatt gattggttaa agccatcaac ttttgtaata tcagcacctt
    28021 tatccttagc taatttacca taattagatg tgaatgtttg tgctaacttc ttacctttaa
    28081 catcatcaaa atctttaata tctttttcat tttcacgaat aactaaaaca gcacttgaga
    28141 atgtgtaagg cttagaaaat ttatatttct tttctctatc aggattaata cctacttggt
    28201 tcgcgattac atcaaaacgc cctgcgtcta aacctgcaaa catagaatcc caagaagttt
    28261 cattaaattt aagttttaaa ccttcttctt tagccactgc tttaataaca tcaatatcgt
    28321 aaccagttaa tttaccatct ttatcgtgga aactaaatgg tgcataagtc ccctcagtcc
    28381 caacattaat tgtcttgcta tctttactag cttccttgtc tttcgacgaa ttgtttccgc
    28441 atgcagccaa tatgaaaacg aaagctatca tcacaaataa aagtcttttc atagaatata
    28501 tctccttatt cttattattc taatcggaat aaaaatattt taatctgaaa tgccggataa
    28561 gtcaatatgt tgaacttttg ctaaaaagct ttatatagct agcaacttat atttattgac
    28621 gagaatcaac ttcttttttc ttcccttgta acataaatga acaaaagaaa ctaataactg
    28681 taatcaataa tgcaaataaa aatccagcgt gataaccatg caacatcgta tcaactttaa
    28741 tttgattcat catatcaatt ttgttcatgc cattatagtc tgacatagtt ggagaaaagt
    28801 ttttgcttgc atgagataaa atagtaatta atgcagctgt tccgatagaa ccagcaattt
    28861 gttgtactgt gtttgtcata gatgaaccat gtgcattcat ttctggcttt aattgattca
    28921 tcgtatgggt cattactgga attaatccta acgcgatacc aaccattcga atcgcataaa
    28981 ctgttgccaa catgattgtt gatgtttgtt catccattac aacaaaataa gccgtagtaa
    29041 taacaacaat cgccataccg actaaagcca attttctagg accaaacttt tcatataaag
    29101 cacccgaagt catcgacata aatgccataa caattgctcc tggtaataaa gttaacccag
    29161 aatctaaagc tgaacgatgc aataaatttt gcacaaagat aggtaaaatc gtttccgaac
    29221 caatcatcga taccattgtg acacccataa taatgatacc aactgtaaat tgtttatttt
    29281 taaatacagc aaaatttaat aatggtgttg atagccgata ttgacgtaat acaaataatg
    29341 cgactaaaac aacgcctcca atcatagata ataatacgat aggattatca aaaccttttt
    29401 ctgaaattga actcgttcca tacaataacc cgccgaaacc tagtacagaa aaaatgacag
    29461 aaataaaatc aataggtact ttaattgtag ttccaacatt ttttatcgtt ttaaatccaa
    29521 ttaaaaatgt cacagctgca attggagcga caacatggaa aagcgatctc cagttaaaat
    29581 attcaacaag gtaacctgaa agagtaggtc caatagctgg tgctaaacca attaccaaac
    29641 caaatatccc catatattta ccgcgttcat gcggttggaa aacatccaaa atcgttgtca
    29701 tcatcaatgg catcataata cctgcgccta acgcctgaat aattctagca actaataatg
    29761 ttgcaaagtg cggactcaag ccgccgagta atgtacctaa gaaaaagata gaaataccca
    29821 ctaaaaacac ttgtcttgtt gtataacgtt gtataacaag cgcggacaaa ggaataacaa
    29881 tcccattagt caataaaaaa gctgttgtca gccattgaac ttgtgtatat gaaatttcaa
    29941 aatctttcat aatacttggt aaagccgtca ctaatagtgt ttcatttaat aatccaaaaa
    30001 atccacccat cagcatagta ataatcatcg ttaatttttg tttcttcgac atttaaagct
    30061 tccccctcac catctaaaaa acgacacaca tttttagata tccggtaatt atacccattt
    30121 tccaaaaaat tagcacgact ttattacaat ttctttaaaa cgtaatcatt actatttaca
    30181 tatgagtagt tatcggtcat aatatattta caaggaggga tagagatgaa attagattta
    30241 cagactgctc gtcgaaattt aaatagccct aacattaaaa caagaaagcg tgctttaaag
    30301 attattaagc aacacaaaag agcgaaataa ttgttttaat ttgactatat tgtatgtcta
    30361 actatatttt cgagtatccc tagctcgtta atataaactc tatacttaac tatttcctta
    30421 cattacttca catatttaag agctgcatat acactcactc cttgtgtaca tgcagctctt
    30481 tttttataag tagtatttat ctataacttc taaatcatcc gttaattcat aaacaagcgg
    30541 tgcacctgtt ttaatttcat aattaatgat atcttcatct gacacatctt caagatattt
    30601 aattaatgcg cgaattgaat ttccgtgtgc agaaactaat accgtttgac catctagcaa
    30661 atattgtgaa atatgatctg tccaaaatgg tatcactcga actaaagtat ctttcagact
    30721 ttcagaataa ggcatcatac gtttatctaa atgattatat cgacgatcag ctaagtaagc
    30781 ttcacgttgt tcttcggttt cagcaggtgg tttcacatca taagaacgac gccaaatatg
    30841 tacttgttct tctccaaatt cttttctagc atcatcttta tttaagcctt gcaatccacc
    30901 atagtggcgt tcatttaaac gccagctttt atatacagga atccattgtt gtttagattc
    30961 agttaaaata taatgcgttg tatctaaagc acgtgttaat aacgatgtaa aagctacatc
    31021 gatggcaatg ttattttctc ttactttttc acctgctcta gtcgcttcat taataccttg
    31081 ttcagataaa ttaacatctt cccatccagt aaataagttt ttagcattcc actcgctttg
    31141 tccatgacga cataaaatta attttggcat aacttataac ctctttcctt taatctttag
    31201 gacgttattt cagggatatt ttaagatgat ttaatcttag taattttctt ttcaatatat
    31261 tttaaaattc aatgattaaa tcaattatca ctagacatta aatacataaa tcctattccc
    31321 cattttcatt ttttaattca taaatgaatc aataaccacc taataacaaa tcatattata
    31381 cacctttgtt cgctattttt ctaaggttta aaaaatattt ttaggtaagc ctaaaaatag
    31441 atgtaataaa aacgcctcct cagatattta tatatctatg aagacgttta aatacattat
    31501 agatggtctg gttctgggtg aacgtatact gaggaaatac cttttttgtg caaatgatgt
    31561 tcgacattgt cacaaatttg atgcgcttct actaaggaaa ggttagcatc tacaacaatt
    31621 gtgacatcaa taaacacact acttccatgg taacgccctt taatactttt aacttcttgt
    31681 acttcatcaa cttctaaaat atcattgcga tacgcttcta attcagtttc attgaaacca
    31741 tcactcaaca taaaaattgc ttctttaaaa ataccaaaac cagtataaac gattagtaag
    31801 cctagtaatg ttgctaaaat aatatcgaca attgggaaac cgatttgcgt aaaaattaat
    31861 cctatcgctg ttccaatgct gactaaacta tctgataaat tatctttggc agccgaattt
    31921 aaagaactac ttttcgttct tttcgctagt ctttgattga ctgcaaatac aatcaacatt
    31981 acaagaccac tgattaagct gacgataatt gttattgcgt taggtacaac gtcatcttct
    32041 ttgaacaaac gaggtgcatt ttgaataact acttggatac ctacaaacat aatgacaaat
    32101 gacaccaata atgaagaaat attttcagac ttcaaatggc cataaggatg atttcgatct
    32161 gcaggtttaa ttgaaatttt caatccaata ataacagcta aagaaacgat aatatctgtc
    32221 atattgttta atgcgtcggc tcttacagct gcagagttaa agacaaaacc cgtgacatac
    32281 ttaacaatag ataagattat atatacaatt aaactcaaat aagcaccgcg ttgcgccaat
    32341 ttaagatttt cattatgaga catgcgttga accaccttga attagtatag taacaatatt
    32401 atgaatgatt tattttaaat ttacaacgtt tttaattttt ataaattttt ataaaattaa
    32461 actaatttat tcattcgcaa cccctaaaaa taatttttag cctttctgcg aattttatga
    32521 gctagaaagg cttatgcatt agtagcgaag ctaactaatg cgttccttta ttttctgcga
    32581 attttatgag ctagaaaggc ttatgcagtt gacgttttac gtccaactgc gttccttcgt
    32641 cttcttcaaa tttatttgtt agaaaggtgc ccaacccttc ctgcttgtta actttcgtct
    32701 cgaaaatttc tatgttggga ccctatgcat tagttgcgaa gctatctaat gtcatgacct
    32761 aattatatag aagaaaaagt gcatcagtga caaattaaat gagatttcta ctctaccaaa
    32821 ctctcttcga aagacaattt tctcctctat ttattagcaa actattgcat ttctccatat
    32881 agtacttcct tacttaaaat acgctgaatg ttctgaatta aacgcttttt tacatagtta
    32941 acactagtta atctattagt taacattagt taataattag ttaatttcca tttgtattct
    33001 catgtgataa attctaaaag catacaataa atttaatatg taaaaagaaa gggaatacac
    33061 atgaaaaata aatatatctc gaagttgcta gttggggcag caacaattac tttagctaca
    33121 atgatttcaa atggggaagc aaaagcgagt gaaaacacgc aacaaacttc aactaagcac
    33181 caaacaactc aaaacaacta cgtaacagat caacaaaaag ctttttatca agtattacat
    33241 ctaaaaggta tcacagaaga acaacgtaac caatacatca aaacattacg cgaacaccca
    33301 gaacgtgcac aagaagtatt ctctgaatca cttaaagaca gcaagaaccc agaccgacgt
    33361 gttgcacaac aaaacgcttt ttacaatgtt cttaaaaatg ataacttaac tgaacaagaa
    33421 aaaaataatt acattgcaca aattaaagaa aaccctgata gaagccaaca agtttgggta
    33481 gaatcagtac aatcttctaa agctaaagaa cgtcaaaata ttgaaaatgc ggataaagca
    33541 attaaagatt tccaagataa caaagcacca cacgataaat cagcagcata tgaagctaac
    33601 tcaaaattac ctaaagattt acgtgataaa aataaccgct ttgtagaaaa agtttcaatt
    33661 gaaaaagcaa tcgttcgtca tgatgagcgt gtgaaatcag caaatgatgc aatctcaaaa
    33721 ttaaatgtaa aagattcaat tgaaaacaga cgtttagcac aacgtgaagt taacaaagca
    33781 cctatggatg taaaagagca tttacagaaa caattagacg cattagtagc tcaaaaagat
    33841 gctgaaaaga aagtggcgcc aaaagttgag gctcctcaaa ttcaatcacc acaaattgaa
    33901 aaacctaaag cagaatcacc aaaagttgaa gtccctcaaa tccaatcacc aaaagttgag
    33961 gttcctcaat ctaaattatt aggttactac caatcattaa aagattcatt taactatggt
    34021 tacaagtatt taacagatac ttataaaagc tataaagaaa aatatgatac agcaaagtac
    34081 tactataata cgtactataa atacaaaggt gcgattgaca aagctgtatt aactttactt
    34141 ggcgatggtt ctaaatctta tatccaacca ttgaaagttg atgataaaaa tggctattta
    34201 gctaaatcat atgcacaagt aagaaactat gtaactgagt caatcaatac tggtaaagta
    34261 ttatatactt tctaccaaaa cccaacatta gtaaaaacag ctattaaagc tcaagaaact
    34321 gcatcatcaa tcaaaaatac aataactgga ttatttaact cattctggaa ataatcaatc
    34381 aaaaatatct tctctagttt tacatcattt tttaaataat tttcgtaata aaccgtgatt
    34441 aaaaagaacc gctgattctc aatcgaatct acggttcttt tttcattttc catcaattaa
    34501 atgtttcttc gctatttgtc agcccacttt tttacctgca acttgttaaa taatccttac
    34561 atcgttaacg aatagttcat catttagttg aatcagctca actttattaa cttcaaattt
    34621 tcacaaacta ttgcgcaatc cattcctctt ccactacaag caccataatt aaacaacaat
    34681 tcaatagaat aagacttgca aaacatagtt atgtagctat ataaacgcct gcgaccaata
    34741 aatcttttaa acataacata atgcaaaaac atcatttaac aatgctaaaa atgtctcttc
    34801 aatacatgtt gatagtaatt aacttttaac gaacagttaa ttcgaaaacg cttacaaata
    34861 gattattata tatatgaact taaaattaaa tagaaagaaa gtgatttcta tgattaaaaa
    34921 taaaatatta acagcaact
//
LOCUS       DAMZLE010000012        34323 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMZLE010000012 DAMZLE010000000
VERSION     DAMZLE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.012"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            8..322
                     /locus_tag="POB92_000583"
     CDS             8..322
                     /locus_tag="POB92_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578105.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(328..465)
                     /locus_tag="POB92_000584"
     CDS             complement(328..465)
                     /locus_tag="POB92_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDI6578106.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(910..1365)
                     /locus_tag="POB92_000585"
     CDS             complement(910..1365)
                     /locus_tag="POB92_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578107.1"
                     /translation="MMFITGIVISLVVILIASYFSFVLTTLIISEDFSDRVTLKVAFI
                     LPIILISLSIRACLENLRSNKQLAKTAFKMATTQYPSVVTMFIGMVKDHQAQVHCFGE
                     STYTYNQNKENLILKKDINKSWLNSLKNLKSLIKSEGFKKNFLNNLAEA"
     gene            complement(1362..1949)
                     /locus_tag="POB92_000586"
     CDS             complement(1362..1949)
                     /locus_tag="POB92_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578108.1"
                     /translation="METKDLSLKIDATKIEVAGIKNKFTAQCKLAYSFIATIATIFAT
                     IYSDGKQLVIFSMLLFVFPSFIESFGIANENKVITAIVKIYKYIFFIVSLFLFIIFVW
                     YFYDKQDAYNYTKVIGDILLIVSIFINLVYSLISYLNLRYNINEHIALAAVEQSTLEK
                     QKERLHFQDNIKKAHKKVYREFVSKQNKDKKGKDK"
     gene            complement(2339..3784)
                     /locus_tag="POB92_000587"
     CDS             complement(2339..3784)
                     /locus_tag="POB92_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDI6578109.1"
                     /translation="MQAKMTKEEFIEWLKTSEGKQFNADLWYGFQCFDYANAAWKVLF
                     GLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHTYDFPMWFIRPNFKSEITPRSV
                     QSPTQAPKKETAIPQPKAVELKIIKDVVKGYDLPKRGGNPKGIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQLGNKYYYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
     gene            complement(3765..4202)
                     /locus_tag="POB92_000588"
     CDS             complement(3765..4202)
                     /locus_tag="POB92_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6578110.1"
                     /translation="MDINWKLRFKNKAVLTGLVGALLLFIKQVTDLFGLDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSNKEEQQVTWKSSQNSSLT
                     PELSTKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKND"
     gene            complement(4258..4653)
                     /locus_tag="POB92_000589"
     CDS             complement(4258..4653)
                     /locus_tag="POB92_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578111.1"
                     /translation="MEGNFKNVKKFIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRISQTNLGSSDLGISGVNSGVAS
                     KNINFSNPSGMLYVTISDVYSGSPTLTIE"
     gene            complement(4659..5831)
                     /locus_tag="POB92_000590"
     CDS             complement(4659..5831)
                     /locus_tag="POB92_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDI6578112.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCQFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGATGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVKKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQSNSISESVLQQIGSKVSIEDFEEFKQTLNDLYAPKNHNH
                     DERYVLSSQAFTKQQADNLYQLKSASQPTVKIWTGTENEYNYIYQKDPNTLYLIKG"
     gene            complement(5844..7742)
                     /locus_tag="POB92_000591"
     CDS             complement(5844..7742)
                     /locus_tag="POB92_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI6578113.1"
                     /translation="MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPEKST
                     PAPKPEDDSDNNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMIKYEIPLSIENLKADDSIWRSMLEHVNWNM
                     IDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGLASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSSLNKILKGKGTLNNQGKAFAEACRKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGI
                     YFTRDKYK"
     gene            complement(7879..8178)
                     /locus_tag="POB92_000592"
     CDS             complement(7879..8178)
                     /locus_tag="POB92_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDI6578114.1"
                     /translation="MFGFTKRHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIK
                     LDKTLDAIQRERQIDEKNKKENDKNIRDMKMWILGLIGTIFSTIVIALLRTIFGI"
     gene            complement(8219..8392)
                     /locus_tag="POB92_000593"
     CDS             complement(8219..8392)
                     /locus_tag="POB92_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDI6578115.1"
                     /translation="MKMIYPTFKDIKTFYVWGYYKNEQIKWYVDKGLIDKEEYALITG
                     EKYPETKDEMSQV"
     gene            complement(8396..8773)
                     /locus_tag="POB92_000594"
     CDS             complement(8396..8773)
                     /locus_tag="POB92_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDI6578116.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASNQQSESDLSDEELRGMVASMQMQVAQVNVLTMELAQ
                     QNAMLTQQLTELKINKTSTEGDV"
     gene            complement(8773..10596)
                     /locus_tag="POB92_000595"
     CDS             complement(8773..10596)
                     /locus_tag="POB92_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDI6578117.1"
                     /translation="MVVDNFSKDDNLIELQTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNKPLSISKHNAMTSIVLKTDNFDDEHGAYISDELTIVDAINGRMQYVIPNEFLK
                     YTGRVHAQAYFTQNGSNNVIVERQFSFNIENDLISNFDGKTKLVYIKSIQDLTESVKE
                     EVEDLKKSLSDTKSLVTEIDSRINQGIQRLEIKQNEAVQMITTTQDKAVQYINSEFQK
                     IVDKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESSEQSIDSVLS
                     TVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWHPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNTLNQAKQYVD
                     GKLQSISWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPSNSKKVYTRSITNGRLDSQWATPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            complement(10596..12506)
                     /locus_tag="POB92_000596"
     CDS             complement(10596..12506)
                     /locus_tag="POB92_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578118.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     DHKTLQDRLYHDYSTLDAFTKKVEKAVDEHYKEYRATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     GNELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPVENL
                     MIFRREYKPTERQLKNSLNFVEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGSWNFNPQSAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESKRFSDFPKDYKGIAGWVLEVKSNTPGNTTQVLRRNNFASAHQFFVRNFGTGGN
                     SGWSIIEGKEVE"
     gene            complement(12521..14422)
                     /locus_tag="POB92_000597"
     CDS             complement(12521..14422)
                     /locus_tag="POB92_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6578119.1"
                     /translation="MTITIKPPKGNGAPVSVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            complement(14431..15378)
                     /locus_tag="POB92_000598"
     CDS             complement(14431..15378)
                     /locus_tag="POB92_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6578120.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVPCFCKVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTHPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFNGVINPKQKGNKVSFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRKYTFENLSKGTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDRIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            complement(15391..18858)
                     /locus_tag="POB92_000599"
     CDS             complement(15391..18858)
                     /locus_tag="POB92_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578121.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ANDKKLQKTIKVAKKSLDAFSNKKVKAKLDAKIEDLQQKALEASFELNQLDSKEVTPE
                     VKLQKQKLTKDISEAEAKLSELEKKRVNIDVNADNSKFNRVLKVSKASLEALNRSKAK
                     AILDVDNSVANSKIKRTKEELKSIPNKTRSRLGVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLNDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVLKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVT
                     VWNVLKAVASVVWNAILKAIITAVTNVYNFIMIVWNQIVAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTAISTALQAAWSFISNIWNTIWSFL
                     SGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVVQG
                     FVNVVNKVSQGMTNAVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKK
                     ALDAAKSALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPT
                     LNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRN
                     AKRNSEYYL"
     gene            complement(18875..19219)
                     /locus_tag="POB92_000600"
     CDS             complement(18875..19219)
                     /locus_tag="POB92_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578122.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            complement(19249..19614)
                     /locus_tag="POB92_000601"
     CDS             complement(19249..19614)
                     /locus_tag="POB92_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDI6578123.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVEAEEKPAEA"
     gene            complement(19677..20258)
                     /locus_tag="POB92_000602"
     CDS             complement(19677..20258)
                     /locus_tag="POB92_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDI6578124.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDS
                     AKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKEDALTSEDLTAIPQPKVDSSSVTPGEV"
     gene            complement(20277..20660)
                     /locus_tag="POB92_000603"
     CDS             complement(20277..20660)
                     /locus_tag="POB92_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578125.1"
                     /translation="MTPNLQLYNKVYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     ITQLVDDTTNQELLHTSVTISYKTF"
     gene            complement(20672..21019)
                     /locus_tag="POB92_000604"
     CDS             complement(20672..21019)
                     /locus_tag="POB92_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDI6578126.1"
                     /translation="MNIDGIDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            complement(21019..21321)
                     /locus_tag="POB92_000605"
     CDS             complement(21019..21321)
                     /locus_tag="POB92_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578127.1"
                     /translation="MRYEDRAIFQLEQVATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDLKNDVSVLRIKESISYPVSHVLINGIRYKIIDTRIYRHETSYYIEEVN"
     gene            complement(21318..21650)
                     /locus_tag="POB92_000606"
     CDS             complement(21318..21650)
                     /locus_tag="POB92_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDI6578128.1"
                     /translation="MTTLDDVKKRIGLKDNKQDEQLEEIIKSCESQLLSMLPIEVEEI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGRAVFF"
     gene            complement(21659..21946)
                     /locus_tag="POB92_000607"
     CDS             complement(21659..21946)
                     /locus_tag="POB92_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578129.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYDPTDERIKSLA
                     SVENKRNEQMIYVVDDKLTKKELIEIASLASLDVDEKQTKAEIISAFESLV"
     gene            complement(21968..22942)
                     /locus_tag="POB92_000608"
     CDS             complement(21968..22942)
                     /locus_tag="POB92_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6578130.1"
                     /translation="MEQTQKLKLNLQHFAGNNVKPQEFNPDNVMMHEKKDGTLMNEFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDSLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADAKPSSNPGEV"
     gene            complement(22956..23576)
                     /locus_tag="POB92_000609"
     CDS             complement(22956..23576)
                     /locus_tag="POB92_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDI6578131.1"
                     /translation="MEENKLKFNLQFFADQSDDPDEPGGDGKKGDPEKKENDEGTEIT
                     FTPEQQKKVDEILERRVAHEKKKADEYAREKAEEAAKEAAKLAKMNKDQKDEYEREQM
                     EKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAV
                     KKAVNEAVKVNARQSPLTGGDSFNHSSKNKPQNLAEIARQKRIIKN"
     gene            complement(23564..23660)
                     /locus_tag="POB92_000610"
                     /pseudo
     CDS             complement(23564..23660)
                     /locus_tag="POB92_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23685..23855)
                     /locus_tag="POB92_000611"
     CDS             complement(23685..23855)
                     /locus_tag="POB92_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578132.1"
                     /translation="MSNTDKYLRDIARELKGIRKELQKRNETVIIDANLDSVRSAVLA
                     DKEKPKYNEPLF"
     gene            complement(23928..24923)
                     /locus_tag="POB92_000612"
     CDS             complement(23928..24923)
                     /locus_tag="POB92_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDI6578133.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKDFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDSRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKPSKKRKTKYFGGKVKSGD"
     gene            complement(24930..26465)
                     /locus_tag="POB92_000613"
     CDS             complement(24930..26465)
                     /locus_tag="POB92_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6578134.1"
                     /translation="MLKVNEFETDTDLRENRNYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIKHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMSDNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKEVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTVERNSIAGVRYLRTKPIDKTDEDEVFTVDLFTSHGVYRY
                     LTSRTNGLKLTPRENGFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTRSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            complement(26476..27771)
                     /locus_tag="POB92_000614"
     CDS             complement(26476..27771)
                     /locus_tag="POB92_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDI6578135.1"
                     /translation="MYEILDLKNKIGGGYNKFWHNKNFYRVVKGSRGSKKSKTTAINL
                     IYRIMKYDWANILVVRRFSNTNKQSTYTDLKWATNQLGVAHLFKFNESLPEITYKPTG
                     QKILFRGLDDPLKITSITVDTGILCWAWFEEAYQIETFAKFSTVVESIRGSYDSPEFF
                     KQITVTFNPWSERHWLKPTFFDEETKLNNTFSDTTTYRVNEWLDKVDIERYEDLYIKN
                     PRRARIVCDGDWGVAEGLVFDNFKVEDFDWFVEFKRTQEITHGMDFGFSQDPTTVVST
                     VVDLKNKKLFIYDEHYEKAMLTDDIKQMLIKKGLDDVDIAADYGAGGDRVISELKSKG
                     IKGIRKALKGANTILPGIQFIQGFEVIIHPSCEHAIEEFNTYTFDQDNDGKWLNKPID
                     ANNHIIDALRYSLEKYHIVRKKRKKNIESKTKVIKSLGL"
     gene            complement(27774..28268)
                     /locus_tag="POB92_000615"
     CDS             complement(27774..28268)
                     /locus_tag="POB92_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6578136.1"
                     /translation="MNKLTKKQRLFVEVYTIPGTECYGNATKSAVHAGYSEKTAYSQG
                     QRMLKNVEIQNYIKEVETKLFDENIMSGKEVLYRLTRTARGEHTEVEAVVTKTGDYKE
                     NPDTGKMQLVYDEHIQLVTKSPKISDQNKALEMLGRHHKLFTDKQEVDHKIPMFVDNI
                     PEDD"
     gene            complement(28455..28874)
                     /locus_tag="POB92_000616"
     CDS             complement(28455..28874)
                     /locus_tag="POB92_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6578137.1"
                     /translation="MSLRKSTQRYLESELSNYNYFDKDIARVRDEVLNPWSQQDTNIG
                     GDRVQSNVSVTEIKAIRVVNDRRLSQLARMKSAIEVVYNHSTTETQKLMELYYFKKPR
                     TLNLTGVAQEINVSKSTAYDMRKDILVRLADELGIIH"
     gene            complement(28889..29035)
                     /locus_tag="POB92_000617"
     CDS             complement(28889..29035)
                     /locus_tag="POB92_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578138.1"
                     /translation="MMWLVIAIILLVILLFGVMLQAEQLKGDVKVKEREIEILRSRLR
                     HFEG"
     gene            complement(29036..29209)
                     /locus_tag="POB92_000618"
     CDS             complement(29036..29209)
                     /locus_tag="POB92_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HDI6578139.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANNDVEMPSDFAKL
                     SDQSDLMRAEVSE"
     gene            complement(29206..29592)
                     /locus_tag="POB92_000619"
     CDS             complement(29206..29592)
                     /locus_tag="POB92_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578140.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            complement(29589..29795)
                     /locus_tag="POB92_000620"
     CDS             complement(29589..29795)
                     /locus_tag="POB92_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDI6578141.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            complement(29832..30359)
                     /locus_tag="POB92_000621"
     CDS             complement(29832..30359)
                     /locus_tag="POB92_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDI6578142.1"
                     /translation="MNNLTVDQLKELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWFN
                     TLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQQADNMEEILGYLDDGDFNDYIE
                     RVEIDFNDSDVVDEFMSTIDEMYESPYSSNLFLPFALANNYYTIDQLIDAYKKKMKRN
                     HERQDGTADAGKGYV"
     gene            complement(30374..30535)
                     /locus_tag="POB92_000622"
     CDS             complement(30374..30535)
                     /locus_tag="POB92_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578143.1"
                     /translation="MLEIIDQRDALLEEKYLNDDWWYELDYWLNKRKSESEQIDIDRV
                     LKFIEELKR"
     gene            complement(30536..30817)
                     /locus_tag="POB92_000623"
     CDS             complement(30536..30817)
                     /locus_tag="POB92_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578144.1"
                     /translation="MEWKRLKNVVPHPVIKNKNLKSVYVTKDNVKEVQKELGFFEIFN
                     EEVLLTGFLSFQRIPIYIIWINPKSHKTPRYYFANEHEIERYFEFLEDE"
     gene            complement(30818..30991)
                     /locus_tag="POB92_000624"
     CDS             complement(30818..30991)
                     /locus_tag="POB92_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578145.1"
                     /translation="MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIID
                     FITKPIYLIKGDE"
     gene            complement(30981..31232)
                     /locus_tag="POB92_000625"
     CDS             complement(30981..31232)
                     /locus_tag="POB92_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6578146.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEVEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQENEY"
     gene            complement(31247..31489)
                     /locus_tag="POB92_000626"
     CDS             complement(31247..31489)
                     /locus_tag="POB92_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HDI6578147.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKMFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            complement(31493..31861)
                     /locus_tag="POB92_000627"
     CDS             complement(31493..31861)
                     /locus_tag="POB92_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578148.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKQKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            complement(31874..32183)
                     /locus_tag="POB92_000628"
                     /pseudo
     CDS             complement(31874..32183)
                     /locus_tag="POB92_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            complement(32192..32410)
                     /locus_tag="POB92_000629"
     CDS             complement(32192..32410)
                     /locus_tag="POB92_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578149.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDVRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            complement(32417..33301)
                     /locus_tag="POB92_000630"
     CDS             complement(32417..33301)
                     /locus_tag="POB92_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDI6578150.1"
                     /translation="MTGWIKLHRKLLDSPIFQNEKLFKVFAYCLMKASHKDHTQLVGR
                     RVVELEKGQFVFGRKRASEELRLKESTVRDYIKLLENLGTIVVKSDNKFSVITVVNWA
                     IYQSMEENSDSKNDNKSTTNGQQINNKSTTNGQQINTNKNVKNGDNVKNGENEKKKVT
                     AFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDQIVTASLKIAKDRNKVTWGYAKSIL
                     NTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDE
                     NLEKDREAFIKRLNSKWE"
     gene            complement(33331..33801)
                     /gene="ssb"
                     /locus_tag="POB92_000631"
     CDS             complement(33331..33801)
                     /gene="ssb"
                     /locus_tag="POB92_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6578151.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLTGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANVPIEIDDNDLPF"
     gene            complement(33802..34287)
                     /locus_tag="POB92_000632"
     CDS             complement(33802..34287)
                     /locus_tag="POB92_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6578152.1"
                     /translation="MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEP
                     VAFLLQSTLGYKVLYVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLAN
                     RIMESHFSLEHAIGMLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYVGG
                     L"
BASE COUNT         9570 a         6824 c         5013 g        12916 t
ORIGIN      
        1 gattaacatg tattttgttt tagcaatatt tacaataatc agtgccagtg taagtttagg
       61 ttattcaatt caagcatgtg catctagcca taatataaat gcatattatg cacttagtcg
      121 aagcttacct ttatttttat tagctatttt ttctttagtc attcatagtg ctatattttt
      181 gataactata tccattgcaa tgatcttagt tcaattttta gatgcgattg ttggttataa
      241 aagtgaagat gtctttaaaa cttatggtcc attagcaaca tctgtagtga acttaatatt
      301 attaatagtt ttcttatttt aactcactta tacaacgaat cttaatcgaa ctaatatcga
      361 attatctaaa gaatatttga tgtagctttc aattaattta ataaagacca gcactctaat
      421 gccacaatca tgttgtattt gtgttgtcgc tttatccacc atcaatgatt attttttaca
      481 ccaatcaaaa aatcgaactg atataaataa gtacaaagct tatctatcaa tccgatttag
      541 ttataaaaca aaaaaagcca cagtaatgtg gctttttgtt atattcagta tcaaaatggt
      601 atcaatagcc attttcggaa gtcaagaatg gcttaacaac gcggtttaaa gctatccaat
      661 actaccttcc atttcgattg gaaaaactaa tttttaagga cttattttta tagaaacgtt
      721 gatttaatgc gatttaaaat gaagttattt ctctcgaaat tttgaggtta ttattttttg
      781 gtatcaaaaa tggtatcata tgtagttatt ttagcttcgc atattaaaat aaccacactc
      841 ctaaattaat aggtggtgtg gttttacatg ctgagcaaaa ttcataatca tcgaaggtaa
      901 tacgagaact taagcttcag ctaagttatt taaaaaattc tttttaaatc cttctgattt
      961 aataagtgac ttcaaattct ttaatgaatt taaccatgac ttatttatgt ctttttttaa
     1021 tattaggttt tctttatttt ggttataggt ataggtagat tcaccaaaac agtggacttg
     1081 tgcttgatgg tctttaacca tacctatgaa cattgttacc actgaaggat attgtgtagt
     1141 agccatttta aatgctgttt tagcaagttg tttattactc cttaggtttt ctaaacatgc
     1201 acgaatggat agggatatta atattattgg taagataaat gctactttta aagtaactcg
     1261 gtcactaaaa tcttcgctta taattaatgt tgttaaaaca aaggaaaaat atgaagctat
     1321 caagattaca actaacgata tcactattcc agttataaac atcatttatc cttccccttt
     1381 ttatctttat tttgtttact aacaaattca cgatatactt ttttatgtgc ttttttaata
     1441 ttatcttgaa agtgcaatct ttctttttgt ttttctaacg tactttgctc tactgcagct
     1501 aaagcaatat gttcatttat attgtagcgt aagttcaaat aagaaatcaa agaatacact
     1561 aaattaataa aaatactgac tattaataat atatcgccta taacttttgt ataattatac
     1621 gcatcttgct tatcgtaaaa ataccaaaca aaaataatga atagaaataa agaaacaata
     1681 aaaaaaatat atttatagat tttaactatt gcagttataa ccttattttc attggcaatt
     1741 ccgaatgatt caatgaaact tggaaagacg aaaagtaaca tagaaaaaat aactaactgt
     1801 ttcccgtctg aatatatagt tgcaaatata gttgctatcg tagctataaa tgaatatgca
     1861 agtttacatt gagctgtgaa tttattttta attcctgcaa cttctatttt tgttgcatct
     1921 atttttaatg ataaatcttt agtttccata taaatttctc ctaaattatc gatctagaaa
     1981 attatatact tactaataat gattgtaaac tatttttttg ttcgcaaaaa ccacccagta
     2041 actagtatgg gtggtttaaa tatgcagtca gcttcttact gctttacgca agtaagtcct
     2101 atgcatagcc ggattgacta ccggaaatgt ggttttaagc cagattggtt actggtaatg
     2161 taattacatt ataacataaa aaaataggca agtaccgtag tacctgcctg ttatctacat
     2221 ttaaatcttg agagaaatgt taaaaagttc tagtaaaata atagcacatt ctatctttaa
     2281 atgtaaatag aaagcaggtg tgtaacgcac ctgcttaaat agatatgact atgtcattct
     2341 aactgatttc tccccataag tcacctaata tctgattagg tggggcagaa ccattccatg
     2401 ttctaatagg caagtaataa cgttgcccct cccatgtata tcctacccaa acatgaccat
     2461 cttgtaacat cacttctgta taatcacaat acccaccagg ttggaactga taacccactg
     2521 gacaagataa gaatggcccc acttttctta ctgtgattgg ttgattgccg tttgtgaatc
     2581 tagcactttc ttccatgtag taagtaccat atttattacg tttccatgca ctcgcaactg
     2641 gtttaactgt attacttgaa gcgcttgact cattagagac agtggcaacc ggtattttac
     2701 catccatgta cgccctaatc tgcttgataa agtagtcttt aagttgcaac cgcttgtctt
     2761 ctggcaatag accgcgagtt actgggtcaa aaccagtgtg taaaaccgaa cttctatgag
     2821 ggcatgatgt tgaagtaaat tcattgtgca atctgattgt atttctgttt gctggtaatc
     2881 cccacttttt taataaccta gcacattctt ggaaagttgc ctgttcattt tttaagaatg
     2941 tcgcgttatc tgcgcccatt gattgacaca cttcaatacc gtaataatat ttattgccta
     3001 attggttagc agtatgccaa cctacttgtg attcatctaa agcttgccac actgtgttac
     3061 ctgatacata actatgtgca atacctgcct ctaatctcga taaaggcgcg ttaactaatc
     3121 cgttgcgata agcttccgct gtcgcccctt tgcttcctgc gtcattatga atgacaatac
     3181 ctttaggatt accaccacgt ttaggaaggt cataaccttt aaccacatcc ttgataattt
     3241 taagttctac cgctttaggt tgtggaattg ctgtttcttt tttaggtgct tgtgtaggag
     3301 attgaactga tcgtggtgtt atttcgcttt tgaagttcgg gcggataaac cacataggga
     3361 aatcgtaagt atgttgtcgt cttgtaactt tttcccaacc ccagccgggt tgttcgattc
     3421 cgtcggtcca gccaccgcct agccaattct gctcatatac aatgatataa tctaaagttg
     3481 cttcaattac ccatgctacg tgtccgtatc ctgcaccgta attactaccg aacacaacca
     3541 tgtcgccggg ttgtgccaaa aagtccggtg tattttggta tacagtagct agtccatcga
     3601 aattgtttgc aaatggtata tcttttgcac ctaaaccttt tagaagtaat ccaaacaaaa
     3661 ctttccaagc agcattggca taatcaaagc attgaaatcc ataccataag tccgcattga
     3721 attgtttccc ctcagatgtt ttcaaccatt ctataaactc ttctttagtc atttttgctt
     3781 gcattgtcgc cacctccatg atgatattca ttcacgtcaa aaccaacatc gttagaggcg
     3841 tctgtgaaag gttgtgatgt atcatactct tttggtgctt tcgtgcttaa ttccggcgtt
     3901 aaactactgt tttgtgatga cttccacgta acttgttgtt cttctttgtt actgtctcta
     3961 ggcgcttgat atgtctgtgc tatagatgaa tctgagacgc cttttgacgt tgggtcagta
     4021 ataacgccaa tacctgtaag taacgtgagg atagcgccta taattgcgct agcttgattt
     4081 aattgagtag ataaatctaa tccgaataaa tccgtgactt gcttgataaa tagcaacaat
     4141 gctccaacta aaccagttag tactgctttg tttttgaatc tcaatttcca gttaatatcc
     4201 atttgtttgc tccttttatc caaaataaaa aacgactaaa aaattagtcg tttaaaatta
     4261 ttcaatggtc aatgttggag atcctgaata aacatcactt atagtgacat acaacatccc
     4321 tgaaggatta ctaaagttaa tgtttttact tgccactccg ctattgactc ctgatattcc
     4381 taaatcactt gaccctaaat tagtttgcga aatcctcatt ataccgctac gtacattttc
     4441 tattgtcacc tgataacttt tattaggttc aactccattt attgtccatt ttgctgttga
     4501 ttcttctatg ctatccggat atttattttt aggtaagggt tttattacaa aagatgaagg
     4561 ctttttccat acttggatat ttccagcata tacttttgta tattcttcac cttcgtaaat
     4621 aaacttcttt acatttttaa aattaccttc cataaaaatc accctttaat taaatataac
     4681 gtattcgggt ctttttgata tatatagtta tattcatttt ctgttcctgt ccaaatttta
     4741 accgtcggtt gagatgcgct ttttagttga tataaattat ccgcttgttg tttagtaaaa
     4801 gcttgagatg acaaaacata ccgctcgtca tgattatgat tttttggagc atataaatca
     4861 tttagtgttt gtttgaattc ctcaaaatct tctatactaa cttttgagcc aatctgttgc
     4921 aatacgcttt ctgaaataga attgctttgt attgcttctg ctaattctct taatgtattc
     4981 atagattcag gcgcgctatc aactagttca gcaatttttg aatccgtata cgttttagag
     5041 tcgttgagag ttgtatcttt gattttttta acttcttgca atttatcttc taacccttca
     5101 acatttgcga tattgatttt atccaataac tcaggttctg ctttgatatc tgtatcttta
     5161 ccatcaattt gccacatttt agtgtcagga ttgattgata ctacagtacc gtttttaccg
     5221 gttgcgcctt gttctccttt tttacctgct tcaccttttg caccaggttg tcccggttcg
     5281 cctttatcac ctttcgcacc tttaaatcta ctttcattct tttcgatgta agaaatgaca
     5341 tctttatcta ttttctcttt aaagtctttg ctcaataaat ctgtcgcgtt atcttttaaa
     5401 attctcgtaa tagcatcatc taccaattta acatcgattt cttttgctac agcagattca
     5461 ataccactat caacgatatt gaaagaaaag ttcgcgacat gtattttttc ttcttctttc
     5521 tctaaaaaca gcttacagcg aacataacca gcgtgtttga taaccttttt aggtatcttg
     5581 taggtaagga acccttttac aacatcgtcg ataataaggg gctcattttt gaatatagag
     5641 ccatcttcca taaacaaatg taatctaggt gttaagccat gtgcttttag atcgatacga
     5701 ccttgtttgt cattgatacc tattcttata gatgctgtat tttcatcttc agtgtaaaat
     5761 tgacaaccaa tgtcacctaa gtcaacacca tcatttttta ttctcgtttc aacatctttt
     5821 attttgtaca tttacacacc tctttattta tatttatccc ttgtgaagta gatacctttt
     5881 aagccgattt gtttatataa tttagcgatt gtacttgctt gatgttggca ccactctata
     5941 gcagtagcgt attggtgggt agctggattc ttaggattcc atctaattcg atacaatgtg
     6001 ttttgtcctt tgttgatgta atcttttctt acgaagctag caccgcccat gattgctttt
     6061 gctggagatg tccaaccttt attccttgca aacgtcattg cgtagttagg attgttgtcg
     6121 taagcgccaa tgccgaagta gttgtatact ccatcttttc cgttagcgaa gttacttgtt
     6181 ccatatccac tttctaagaa agcatgcgcg attaaataaa tttcattaat gttgtgcttt
     6241 ctacaagctt ctgcgaacgc tttaccttga ttattcaatg tccccttacc tttaagtatc
     6301 ttattaagcg aactaactga aacaccttga tacttgccta aattaagcat ttggtagcac
     6361 tgcgtgttac tttcccatat tcgtttaaca ttcattgccg agctcgtttg agctcgtgta
     6421 gcgttagccc agccccaagc attagatttt ttcgggttac ctcttgccat ttgtttatcc
     6481 agtgcttgtt tgaatgtata agggcttgtt tcagttataa tctgcggttg tttagatgcc
     6541 aagccattgt tagctgttgg tgatgagtct cttacattcg ctatatcagc gtttttatta
     6601 tctaccataa cttttattct agattttgtt actgttggtt tagttataga atttaataat
     6661 ttttctctgt ttttaaatat attaagtaat gccttttcta atgcttcata tttatcttta
     6721 gggggaacac cgttgtcaat catattccaa ttaacatgtt ccaacatcga acgccaaatg
     6781 ctgtcgtctg cttttaaatt ttcaatactt agaggtatct catatttgat catcatatct
     6841 acagctacaa ccattgcgtg aatttcattg aaaataaatt cgtttttact cgcactataa
     6901 tcttcacata cgtctataac tatataatca ggttcattag gaacctcaaa taccgctctt
     6961 ctaggagccc aaatattatg tctatctaca taaaagtgtg gatattctac atcttgctta
     7021 tatttctttc tactgttata taaactttct accgagctca ttgtttgagc atttctaatc
     7081 attattcctt taggtttttc gagtcgtcga ttaccctcta ctataaaatg ataaatatat
     7141 tctggataat taacttcttg gctagaaatt gtgtacttta tagttgttac atctttccaa
     7201 atcggaactt ttttattatt tttttcgtta ttatcactgt cgtcttcggg tttaggtgcc
     7261 ggcgtagatt tctccggatg atatggtggt ctaacaaaat atttaactcc tccacctggt
     7321 ccatcatgat aagagtgttt gattttatac ggcggacttc ctgttgcatt atttgtatac
     7381 cagttttgat ccacaccata ccaatagtct tttgtgcatg gtcctactac tatattcacg
     7441 tgtcctgccc aaccaccagt ccaaacaccc cagtcgcctg gttgtggtac aaaatctttt
     7501 gtgtttctaa ttatcttgaa atctctacct ctataattag atttttgagc catagcatca
     7561 gcatttcccc atgttctaaa accccaatat ttatcaagca agtaattagg caagtcccaa
     7621 cattgcgctc ccattccaga accaggtaca tcaatagcta ttttgttttt agcgatatat
     7681 aacgcccatt caaccacttc actagctgta ggttttctgt tttttggatt aggtaatccc
     7741 atgtatgcac ctcatttcaa tcaaaataaa aagccagtgc cgaagcactg actcttaact
     7801 gttatttaca tttaccaaac cagaagcacg cccagaagct atatcctaaa atccctttaa
     7861 gcatggtaat cacctccttt aaataccaaa aatagttctt agtaaagcta tgacaatcgt
     7921 actgaagata gtccctatca aaccgagaat ccacattttc atatcgcgta tatttttgtc
     7981 gttttctttc ttattttttt cgtctatctg tctttccctc tggatagcat ctaaagtttt
     8041 atctaattta atgttaactt gctcttgagt tttttgacct aatttaatct cattgagagt
     8101 gctaagcatt gttttatcat tctcttctaa tcttctaatt ctccattcat gttcgtgccg
     8161 tttggtaaag ccaaacatta cgccacctac tttttgttaa attaaaaagc cacaagcatt
     8221 atacctgtga catttcatct tttgtttctg gatatttttc tccagtgatc aatgcatatt
     8281 cttccttgtc gattaaaccc ttgtctacgt accacttaat ttgctcgttt ttatagtaac
     8341 cccaaacata aaaagtttta atgtctttaa aagttggata aatcatcttc attatttaaa
     8401 cgtccccctc agtacttgtt ttgttaattt tcagttcagt caactgttgt gttaacatag
     8461 cgttttgttg agctaattcc attgttaata cgtttacttg tgccacctgc atttgcatac
     8521 ttgcaaccat tccgcgaagt tcttcatcac tcaaatctga ttcactttgt tggtttgatg
     8581 cattcggtac gtcttctttt tcgaaattgc tattgtattt aatttcgccg ttagtgaaaa
     8641 cgaactttct aggttcgaac tcttctttga atttgatagg cacattgtta tcatctacat
     8701 ctaaactatt gcgtaatccg ccagtattta cgtatccgat aacttcgttt ttatcgttta
     8761 ctgtgatttt cattatttcc accccacaat tttatttatc gtaactctgt ttgcattagc
     8821 accagaacct gttttactgc ctaaatcaag gtacacatcg ttatcgattt ttaacgtcgt
     8881 accactttct ttagttatta agcattcata actaccacca ccgttaccgt ctgagtcaac
     8941 tacatttgtt ttacttaatt gaatcgcatt tggtatagag gttaaactga atgcttcaat
     9001 aacaccacct ggataagtac cgcttatgaa tagaattgca taatttgtat aagcttcggt
     9061 taaattaatc cttgttccta caccgtttgc agcaccgtcg aataacacgg ctgttttatg
     9121 ttcgttaggt gtagcccatt gtgaatctaa tcgaccattg gtgattgatc gtgtataaac
     9181 ttttttagag tttgaaggtg tgaagttgaa taacttattt gcatcatctt taacaaatac
     9241 tgataagtag ccttcgtaac tttcaacaat acctggtaaa tcaggtacac ttgttacgta
     9301 ataattccca gcgcccaatg cttctaaatt acctttggcg ttatataagt tcttttggat
     9361 tgattgaccg ttatgttctg ttaacttatg ttgttgccaa cttatacttt gtaacttacc
     9421 atctacatac tgtttagctt gattcagtgt gttgttagat atttcttcaa caaattgctt
     9481 agttaagttt ccatcattct ttttataaaa cgggtaccac gtgccgtaga ttttgtattt
     9541 tgtgtactca tcgtttgaat catctgggtg ccatgttgca cgagcagtat tattatcaac
     9601 aacataaaca actaacacac cagatttgct tgatgtataa gttgattcat cgaacgaaga
     9661 accgtcatca acaccatctt gtccaggctt ctctaacgtg cctatatccg tcttttctgg
     9721 cgcatctgtt gcgttagtaa tatgaataat cctagatgtg ttaactgtgc ttaaaacgct
     9781 atctatggac tgctcagacg attcaattgc tttaccgtaa tcatcagtaa gtttagactt
     9841 ttgccaattt gttgttgaat tacctttaac aaggtcagcg ccattgattt gttgttcaac
     9901 ttcgttaaca cgttcaaaaa tcgcttgctc tttatcaaca attttctgga actcgctatt
     9961 tatatattga acggctttgt cttgtgttgt tgtaatcatc tgtaccgctt cattttgttt
    10021 gatttctaat ctttgaatac cttgattaat acgactatca atttcagtaa ccaacgattt
    10081 tgtatcactt aaacttttct ttaagtcctc aacttcttct ttaacacttt ctgttaagtc
    10141 ctgaattgac ttgatataaa ctagctttgt tttaccgtca aaattactaa ttaaatcatt
    10201 ctcgatattg aagctaaatt gacgctctac aattacgtta ttgctaccgt tttgagtaaa
    10261 ataagcttgt gcatgtacgc gtccagtgta ttttaagaac tcgtttggga taacgtattg
    10321 cattcgtccg ttaattgcat caacaattgt aagttcatca ctaatataag caccgtgttc
    10381 atcgtcaaag ttatccgtct taagcacaat gctagtcatc gcattatgtt tgctgattga
    10441 taacggctta ttattcttag ttaccgcaaa atttaaaaca ccagttcctc tatctgattc
    10501 atagaaactg atgtttgtgt caataaccgg attatattgt gatgttgttt gtaactcgat
    10561 taagttatca tctttcgaaa aattatctac taccattatt caacctcctt accttctatt
    10621 atgctccaac cactattacc accagtacca aagtttctaa cgaaaaactg gtgagcagaa
    10681 gcaaagttat tacgtcttag cacttgtgtt gtgttacccg gtgtatttga ttttacttct
    10741 aacacccagc ctgcaatacc tttgtaatct ttagggaagt cagaaaaacg ttttgattct
    10801 tcggtggtga tatagaagtc taaaccaaca atttttaaat cagacaattt cgtgatgctc
    10861 ttagggatat gttcccaata accagcactt tgtgggttaa aattccatga accgttgttt
    10921 ttcttgttaa agatgtcgat aacacgttca aatttgagca tatttctacc tgtgctgttt
    10981 ctagttagta cttgtcttaa cgcaccatta taatgaccag gcagtacatc aaagaaccaa
    11041 cctgcatctc taaacgcttt cggtaacggg aaatctaacg cattttgtgt gtcttgcgta
    11101 tagatatagt aatgaccaac ttccgtaata tcacttagat atgctgggtt ctgtattggt
    11161 aacggtttaa cacgtccgcc tgaatcagtc atcgatactt gaggtgcaat gttttttaag
    11221 aattggttaa cacctctttg gccgatggaa taaattgagt gatgtctgtt gttaccaggt
    11281 ccaatagtta cccctattaa aagcgctttg cgtcctgttt ctagatcgta atacatatct
    11341 agaccctcag cttcttggaa gtctccttta aagttattat tcacaccgcc aatatcgata
    11401 cgtcgtttaa ataacaattc ttttgttttt atatcgaaac cttgtaagta gttagggttg
    11461 gctgtattcg aatcacctgt ataccaatat aagatacctg catcataagt gataccttgc
    11521 ataggttgtg tatctgaagt gtattccata ggtatatcca tttgatacaa tactttgtct
    11581 atacctttat caatatcgtc agcacttcta acctcaacaa agttcaacga attcttaagt
    11641 tgtctttcag tgggtttata ttcacgtcta aaaatcatta aattttctac cggattataa
    11701 atcgctgacg tatatctgtc gttaaatata ttcggcatga catcttgcat ttcattacca
    11761 taagttattt ctccagttct atattggaaa cgtacaaact tgttgttttt gttactgtcc
    11821 aatacagctg aataaatcca caactcattg ccgatatatc tataggcgtt gtgtgtgccg
    11881 tgtccgccat ttttaactag cagtctatca ataaattgtc cgttaggctt caatctagat
    11941 aacatgtaat gattgcctgg acgcgcttgt gtcatgtaaa taatttttgt tctagggtct
    12001 acccaaaatg attgcattac tgcgttagta tatggcgata aatctgtgat gaattccggt
    12061 tcttgctctt ttggttcgaa tcggtattct gtcgctcgat attctttata gtgttcatct
    12121 acagctttct caaccttttt agtgaaagca tctagtgttg aataatcatg atacaaacga
    12181 tcttgcaatg tcttatgatc ataaccagta ttatcaacac gcgcgtcttt tacttcgttg
    12241 ataccgtcgc cgttatgacc tagtaccata ttgctgaaac ggccgtttag atacgttaaa
    12301 aaatcagaga cgctacttgt gacatttaaa tgctcatact ttatttgctc tccattatgt
    12361 gcaaatacct ctttatttct atggtattca agagagaaat taaaatccgt cagcatgtct
    12421 gaaataagtt taaagttata ctcattttca tctacatatc tgtagtcaaa gactctactt
    12481 aagtctgtaa ttaatttgtt atccatgtct tcctcctttt ctatccgtaa aactggtaat
    12541 aatttttaat aagttcgtac ataataactt catgacccct ctcgttcgga tgcaatccgt
    12601 ctggcatact tgattttctg aacgctggat tatatggctt aaaataatct gtatgatagg
    12661 catcatatac tggtacatcc aattcactac aagccaatat ctgagcgttg acataatcct
    12721 ctaaagttaa ccctagtttg tttttatccg tatctttacg acgtatcgtt gtaccactca
    12781 tagggcattg cctagtagct gtcattacaa gtatttttga agctggatta tttttcctga
    12841 taacttcaat tgcagaacaa aaggcgccgt aaaacgtttt agtgtcggtt ttatcagtgc
    12901 ctatcggtac gcctgcccaa taaccatgta accaatcatc atctgtacct tgtaatatga
    12961 ttaggtctcc tcttatttgc tctgcttgtc tataaatgct gttttctacc gcttctttac
    13021 ctattggaac tgttgccatt gttgcgccac ctcttgcaag gttggtcgtt ttagctttta
    13081 acttcttgcc taacatttct gtgaaattag ttttcgcatg tgatcctcta gctacagaat
    13141 cgccaatcgt tccaatcgtt tttacatctt taatgtttga tttatctata aaatcgtgaa
    13201 cgatagtgcc atctgatgta gttacagtct tagaactcac tttctgttgt ttgtcttcaa
    13261 ttagatcagt tctactcatc aaatcgagtg ttgatttagc tattgacgct actttagact
    13321 tcaagttttc tgccgcttta ctaggattag aaaggttaac atcatttaat ccagaaacat
    13381 agttagctgc agtattaact tttttcatat atcgttgttc tcgattaaac tcaccaagcg
    13441 ttacatcttg cttaacaatt acattgttta tacccctaat cgttttaact tgtactatac
    13501 ggactaaatc attcaaacct agtttggtag attttatttg tactatgtct ccgggttgtg
    13561 ggtctgcttc tggatatgat tctcttaaca ccaaaaagtc caaagacaaa gattgtttta
    13621 acgacttttt caatctcgat tgtaattctt tatccatagt ttcttggtca gtcactttac
    13681 catctttaaa tggttctgcg tggatgtcgc cgtatatttc agctaatgca cttctagctt
    13741 ccattacgag cccagcgtgt tcgaatgttt cttctcctga ataattacca tatcctctaa
    13801 tgaaggtggc gaaatcactt gcatcttcct cgagttttat agcgttggcg ttgacttcgt
    13861 cagaaataaa ataagacgct ttttgatttg caaaaggcgt caatacaaac ttatatctgt
    13921 ctttcttttt gtcatacgtt attttatatt ctaaaccgaa atgttctaat ccctttttaa
    13981 acatttctaa ccttgtatcg ccttcaccac cattttcaaa cttcgaagac ttaaccttac
    14041 cttcgacttc aaaaagcatt ccagtacctt gaaacacaat gttaaaatat ctttctactg
    14101 taaaagatcc tgttacatta acataaatcc tatcaatcat taacttgtct ataggaatct
    14161 ctctagcagt acattcaaca agttgtctgt cgccttctga tttcctatca atgacagtta
    14221 ttacatattc tttcttgtcg ttttcacctt cgacatgact aacaatccat ctttttccta
    14281 tagcgttaat aacttcataa gtatatttgt tttctagaat atcaaaagtt aatacaccgt
    14341 cagcgttaac tttttttact aaagttgttt ctactgatac aggtgcgcca ttacctttag
    14401 gtggtttaat agttattgtc attctgacac ctacttataa taaaatttca aatcaaactg
    14461 aactttttgt accgtttgat taaactcaaa tttattagct ccgtatttaa attttggttg
    14521 ggctatattc gtttcggtac ttatttcaac accgttttta taaactcgga agctatcata
    14581 aacaattctg tctccagctt ttagtttgat cccttcgatt ttcattattt cagcatgcgt
    14641 taaattccat acaaacgatt ctgtatcttc gcctaaaata attgttatct ttttatacat
    14701 gttgaattgg tcgttaggag cactaccatg atagtaaact gtacctttgc tcaaattttc
    14761 aaatgtatac tttcttttgt ctccgcctgc atgccaatca atattaaaat caaacgacca
    14821 caatccaacc tttttgtttt cttctaactc taggcttgtt ccgatacttt caccgtatgg
    14881 taattctgta gtttcgaatt ttagttcaaa agaaacttta ttaccttttt gtttagggtt
    14941 tataactccg ttaaaaataa ctttatactg tttaccattt acataaattt gttgatcgtg
    15001 tcttgaatat tcataatccg ggaagttgtt tttatctaat ttcacgtaat catcagaagt
    15061 aggatgagta aacctgtaat tcaactcttc ttttcttctt atttctcgta aatacatagg
    15121 ttctatgtct gtcgttaacg aatacaacat atctcgcata taagcaatgt ctgaacgatt
    15181 tttaacttta caaaaacaag gaacaactat atctctactg atataattgc tccccattaa
    15241 tatacgaccg ttcatatttt ctttgtcttg atactttgtg ttgatttgca tgctatcaat
    15301 tactatatcg ttaacgataa acccgtattc acttaatttg attacagtac catctttttt
    15361 tgttaattct atgtccattt gtaacctcct ttataagtaa tactcagaat tgcgtttagc
    15421 atttctgccg ttaacaatac tagtaagcgc atcgttattg acatcgaatt caactttaac
    15481 agttttcatg ttcggtgatg tttcaataga atgtgtgtgt tgtacttgcg catttatatt
    15541 tccacctaaa ttacttaagt ttcctgtaat actagaaatg tcaggtgcgt ttaatgtagg
    15601 ttgaaatgca tcaactactt tatctgcaac gttggaaacg ttacggatga ctttacttga
    15661 gtgattatct atacctttaa cgaaacctag cattgaatac ataccaacat ccatgaattc
    15721 acgtgaaggt gagtgaatac ccaaagcact tttagctgca tctaaagctt tcttagcaac
    15781 atttttagct gcatctacta attgaccagc catttgacca atacctctaa ttaaaccacg
    15841 gatcatatca gcacctgctg acacaaaatc tcctataaag ctttttattt tatttactgc
    15901 atttgtcata ccttgactaa ctttgtttac aacattgacg aatccttgaa ccactctatt
    15961 aacaaagtta attagcgtac ttgttatagt agatacccat tgcatacctt tagtcacgat
    16021 gaagttccaa gcttgagaca ttttgtccga tatagttgat acaacttgtg tgaatatact
    16081 tacaacttta ttccaaatcg tcgttaatat accagataag aaactccaaa tcgtattcca
    16141 tatattagaa ataaaactcc atgccgcttg taacgcagta gatatagctg tggtgatagc
    16201 gttccaaacc ttagttgcca cagtaactat agtgttccac aacgtttgta agaacgtcca
    16261 aatagcattc caaattgtca ttgcgatagt catgattgtt gtaaacacag tagttattac
    16321 agtgactaac aaattccaaa tcgtagtagc gattgtaatt atcgtgttcc agattgtact
    16381 taagaatgtc caaatagctg tccatatcgt cataactatt gtcattatcg tcgtgaaaac
    16441 agttgtaatg attgtaacta aaaggttcca tactgttgtt gcaatagcga taatcccatt
    16501 ccatagccct tgcaaataag caactatttg attccaaaca atcattataa aattgtatac
    16561 attagttact gctgtaatga tagcttttaa aatagcattc catacaaccg aagctacagc
    16621 ttttaataca ttccaaactg taaccataaa ggtttttatg gcattccaag catttataat
    16681 aaagtttctg aatccttcat ttttattcca caataaaacg aatatagcta ttaatgcagc
    16741 aattacacca attactattg ttattggacc acctaaaatg ccaaacacag ttactagtcc
    16801 tgtaatagcg tttctaatta atccaatctt accgaataac agttggaata taactgatat
    16861 aatttttaat ggtcctttta ataacatgaa cgcacctttt aaaattgtta atcccgctct
    16921 taataaaccg aacttactta ctaacgcaat gattctacct attaatccgc cacccataaa
    16981 gttagataca gcaagaataa tcggtattaa aaatctaaat gcaccaacta aagttataat
    17041 gacaccaact aattgtgctg tagctggatg cgcctcaaac aagttagcta tccaaccagt
    17101 tattgcaact gcaacgcgta atactgcact agctatagga gccattgctg ttgcgaatgc
    17161 aactaatcct cttgcgatgt ttccaatcaa ttgcattatt agtggtccat ttgtttgtat
    17221 ataactgaca aagtctttaa acccttgaga ttgtcctact tgttcagacc attccctaaa
    17281 cttagctgtc atttgttcaa gagattggaa tatgccagtt gatgatccgc tgaatgcatt
    17341 catcaaattg ttaattccaa cgaaaacatt tttgaaaatg ttgccgatta taggtaaatt
    17401 tgtttttgtg tattcaataa aacgagtaat cgaattttct ccagctgcac tattagccca
    17461 attagagaat gattgaccta atctatccaa ccaatcagcc gaccattgaa acagtggtgc
    17521 taattgtgtg aacacattga ctaatccgtc accgaaacct cctgcagcac ttaatagctt
    17581 gttaaatacc gaaacaccag ttgtattcat catgttgaag aaccttgatg ctacaccgct
    17641 attttgagcc catttaagta cactttgaga cgcctcttcc attcctcttg aaataccact
    17701 aaaaaacggt tgtaagctct gcattgcagt tttaacagta tttaaaccat ttgcaagagt
    17761 tgtgaagata gcggattgat tttgctttat aatatcagtc catgctgact ttacgccatc
    17821 taaagctttt ttgtattcgt ttgttgctga gctagcttgt aaagtgccat cattaagcat
    17881 ctttatagcg ctgattgcca ttgcgccaaa tgctacaaag ccagcgccgg ctattgctac
    17941 cgcaccacct aaagcaagta caccgccagt taacactttg atagcgttta atagcgcaaa
    18001 tactacaggt actacgctcg ctattacagg tattaagata ctaaaagatg atgtaagtaa
    18061 tccaccaacc atattagaac ctacagtacc gaacacacgg aacatattag ctaaattccc
    18121 catctgtctt tggaaattgt cgtttgcttt tattatgtag gcataagctt tctttaaacc
    18181 attagtatcg acatctacct ttgttgtttt tttgtttggc aatgcgtcta atgatttttt
    18241 aaacgcataa atagttggta tagaaagccc tgtatctaca cctagtcgag atctagtttt
    18301 gttcggaata cttttaagct cttctttagt gcgtttgatt ttagaattag caacactgtt
    18361 gtctacatct aaaatagctt tggctttaga cctatttaaa gcttcaagac tagctttaga
    18421 tacttttaac actcgattga atttactgtt atcagcattg acgtcaatat tgacacgttt
    18481 cttttctagt tctgataact tagcttctgc ttcagagata tctttagtta acttttgttt
    18541 ttgtaactta acttctggtg taacttcttt tgaatctaat tgatttaatt caaaacttgc
    18601 ttctaaagct ttttgttgta gatcttctat tttagcatct aatttagctt ttactttttt
    18661 gttactaaat gcatctaaag actttttagc aactttgata gttttttgta attttttatc
    18721 gttagcgttt aattcaacat ctttagtttg atctgctact cgtttaaatc tttgcacaga
    18781 cttaaccgca ctatcaattt gccttttgaa tttggctaca ctagcttcaa tagtcgcttt
    18841 aattttatat tccgtcacat taacacctct ctttctattg cttattaaat tctgctataa
    18901 ctttaaagaa ttcattattt tgtggttcgt attcatcacg ttcgctacta aatcttatat
    18961 ctttaccttc gttaagccgt tggatatttt cttcataagg caatacgtcg tttgcattgt
    19021 taaaaacata ttcctcttta ggtttatttt ctgtcccaac atttttagta gctgcagcat
    19081 cacgaatagc aaacgcaagt ttgtaacgtt cgaattcttg ggttagcatt tcatactctt
    19141 tcgcatacat tcgatagtta tattctgtta atgtcatttg ctcaataacg ttcaaatctg
    19201 taataccaag tgttgacata caagttataa cgattctgtc gtaagttatt aggcttccgc
    19261 tggtttctct tccgcttcca ctacttctac taggtttcgg gtcataggtc gctttcccaa
    19321 ctccgttaaa atatctgaac cgaattcttc tagtccgata ttttctgcga tttcatctag
    19381 cgcttcatca atgttattaa tagtaattgc ttgttttttt aagtgagatg tagctgcaat
    19441 taaaacttcg ccaatcacaa caggatttcc actttctaaa cctacaggca acattgatac
    19501 accttgaccg atagaagctt gttcaacttt taaacctaat cggttatcga tttctcttaa
    19561 aaatttaaaa ccaaaactta actctaatga ctttccatta atttctacat tcataattta
    19621 aaatctccat tcatgattaa tttaaacaaa aataaaaagg gcgttaagcc ctatttttat
    19681 acttctcctg gtgtaaccga tgatgaatct actttaggtt gtggaattgc ggttaaatct
    19741 tcgctagtta acgcatcttc ttttgtagtg tcgtggaatc tgtatccagt cgccttaagt
    19801 ttttttgtta cagcctcagg caatgttgca aatccacgtt ggaaacgacc attcacaccg
    19861 tattcatatt catattcatc aataccgtta gcttctgctt ttaattcaaa tttattgtgg
    19921 aatccttgaa aatatttcgc tttaaattta gcggaatccc catttttgcc tggtattcta
    19981 ctttcaactt cccaagcttc atacaatacg cgatctacaa ctgcatcttc aatttcatct
    20041 gcaaaatcgt caccataaaa cattttagca gtaccagaca tcgttgactc aactgaacca
    20101 ccagtgttat aagaaccgtc cattgtatcc tctgtatctg tatcagcttc atgtgataag
    20161 ccgtattcag ttaaaaaaag cattttagta gcatctactt tttcgccagc ttttctaaat
    20221 aaaataatac gatcattact atttttcata tttgccattc aatattcctc cgttttttaa
    20281 aatgttttgt aagatatcgt tactgatgtg tgtagcaatt cttgattggt agtatcatcg
    20341 actaactgtg tgatgttagt atcatcttct tcaaagtcat aatcgtttgt tttaacgcta
    20401 ggtgttaaat catcaataca tcttttaaca agtccgtcat gatgtcctaa atcatcactt
    20461 acactccaaa tatcaataac taaattcgtg tcaccagaat aactatcaaa cgtgtattta
    20521 cttctgtttg actccggcat ttttattaca aaaaaaggat acggaatctc ttgttgcatc
    20581 tctttacgag aaataacagg gaatccatat ccttgtagcg tttcatacac tttattataa
    20641 agttgtaagt tcggtgtcat gcttttatct cctattcaaa caacgctttc aattcttcta
    20701 cagttgattt tcttattacc tcatatactg gccacataaa aggttctgcc tccatgtatc
    20761 gagtaccaaa ctctaagaaa ccactataag ctgcatgcga tgtgatagtg tattgcaaat
    20821 cgccagtttt tttatatctg atattgcgtg ataaattacc agtccaataa cccttattca
    20881 ttacttctct agctttcaat ttagctcgta ctacatattc tttggcgttt tcctgtaaaa
    20941 tatcatctac atcatcatca atgttggttt tcatatcgtg aaattggttt aacagtgcgt
    21001 ctattccatc tatattcatc aattgacctc ttcgatataa tatgacgttt cgtgtctgta
    21061 tatccttgta tcaattatct tgtagcgaat accattaatt aacacgtggc taacagggta
    21121 agatattgat tcttttatcc tcaggacact tacatcgttt tttaaatcgc caaattcaag
    21181 ttgctttctt gctctagaaa taggattaat attgcatggt atcgcatcat aagtgattag
    21241 cgtgttttct tttttgctag ttttaggatt gtaagttgct acttgttcta attgaaaaat
    21301 agctctatct tcatatctca aaagaacaca gcccttcctt ttttagttct cgttctagca
    21361 ttaaagtaat tatcaataat agcttcatac tccttaaaat cgttcaattc atacgcattg
    21421 ctacgtccgt caaccgcttc tgatgtcata ccttcagcac caatcctgtt gtagcgttta
    21481 actgcaactt ctttgatcat gtaactaaat ctttcaggta tttcttcaac ctcaattggt
    21541 aacattgata acaactggct ttcacaactt ttgattattt cttctaattg ttcatcttgc
    21601 ttattatctt taagaccaat gcgtttctta acgtcatcta atgtagtcat ataaccacct
    21661 acacaagtga ctcaaaagca ctaataattt cagcttttgt ttgtttttcg tcaacatcca
    21721 aactagcaag actcgctatt tcaataagct ccttttttgt taacttatca tcaacaacgt
    21781 aaatcatttg ctcattgcgt ttattttcaa cgctggctaa agattttata cgttcatctg
    21841 taggatcata acctttgcga gggtagacat gccctttcat atagacatgt ctgttatctt
    21901 ctaaatctgt aaaatctact ttaacaattc caatgatttc gggcatgtta ccactcctaa
    21961 ttatttatta aacttctcct ggatttgaag atggttttgc atcagcagga actaacttag
    22021 caaacgcttt atcatcagcg atatgcaatg ctacatgcat agttgcacgt aatgccacca
    22081 tatcttgttc gaataagttt acaggtgtgc catcttcgtt tttaactgta gataattgtg
    22141 ctgtttcatc gattttgtat tcaattaatt gagggatacc gtaaatcaac ttatcaaagt
    22201 caccagtgat taattcaccg cgttttaagt tgcttgattt aaggttaacc acaggtaaac
    22261 cgtctaacga atcactgtta cggtcataaa tgcgttcttt cgtttcagga tctacaattt
    22321 tacgcaacaa gcttctgttt tgtgtttttg agataaacgc atttgcttct aattcgtcat
    22381 cttcaagtaa tgcctctaaa tcaataatgt tatcttgtgt gaagtcacct ttaataacct
    22441 tattagtttt ttcaattgat tgtgcaattg atttaccgaa tggattgtta ccttgattca
    22501 aaatacctgc ttcgtcaaac tttttataga aagcttcagc aatcataggt ttcatttctt
    22561 cgaagaattg tgaataagtg taattcaaga attcttttgt tacaggtaag ataacaccta
    22621 atttaaacgc tctcattgta gcattgaccc aagtagcctt agatgtttca attttttgac
    22681 cttcacctac ccagtaagca cctggtttat cagcccaaaa agtaaacttt ttctcagtac
    22741 cttccattgg ttcgtactta cctaattgca tgattttaga gttttccata acctcttgta
    22801 agataggcgt tgtgaattca ttcatcaacg tgccatcttt cttttcgtgc atcattacat
    22861 tatcagggtt aaattcttgc ggtttaacat tgttacccgc aaaatgttgc aaatttaatt
    22921 ttaatttttg tgtttgttcc atttaaatgc ctccgttaat ttttaataat tcttttttgt
    22981 ctagctattt cagctaagtt ttgcggttta tttttagacg agtggttaaa tgaatcccca
    23041 ccagtcaatg gtgattgtct agcgttaacc ttaactgctt cattaaccgc tttttttact
    23101 gcattagaaa aagcttcaac attcaattta gtttgttcag cagtgtcagt tacaaccaaa
    23161 ttgacaacct catctgatga atcaacttcc gcttcactca acatttttcg tgcttctgaa
    23221 cgcatttcgt ttaattgttt ttctgaacgt aattgttcca gttctttttc catttgttcg
    23281 cgttcatatt catctttttg atccttgttc attttcgcta atttagcagc ttctttagca
    23341 gcttcttctg ctttttctct tgcatactca tcagcttttt tcttttcgtg ggctacacga
    23401 cgttcaagta tttcatcaac tttcttttgt tgctctggcg tgaaagttat ttcagtacct
    23461 tcgtcatttt ctttcttctc aggatctcct tttttaccat ctccacctgg ttcgtctgga
    23521 tcatctgatt ggtctgcaaa aaattgcaaa ttaaacttaa gtttattttc ttccatgaga
    23581 tatacctcca tttatagtct gtcgactgtt tttccatgcg tgctttttat gtcatcagca
    23641 cgttttggac ataaaaaaat gccaacacaa ttaagtgcta gctattaaaa gagtggttcg
    23701 ttatatttcg gtttttcttt atcagctaat actgccgacc ttacactgtc taagtttgca
    23761 tcaataataa ctgtttcgtt tcgcttttgt aactctttac gtataccttt taactctctt
    23821 gctatgtctc taaggtattt gtcagtatta ctcatattaa tatcctccaa acatttaatt
    23881 tactgtcata caaagctaac ttacctttaa aaatctttac ttttaaatca atcaccgctt
    23941 ttcactttcc ctccgaagta ttttgttttt cgtttcttgc ttggtttttt cggccacata
    24001 gatttaggta gtaaagcgca atctgaacga caattgatat gcatagggta gaaattaaca
    24061 ccaattttag cgtctttaac tttgaatatt tctccattaa gccccttgca tactttagtt
    24121 gttctactat caatttttgc aatatacata taatatcctt ctggtgaaat ttctttcatg
    24181 ctgtcaatgc ttgattgtgc gtgaacacgt gccgattccg tataaagcaa tgatttaatt
    24241 gctgcggtct tttgtcgtgc tgtgccttcg aatttattta agtgcttgcg catatcttta
    24301 acatattcat taggatgtcg acctctaata actacattag caattatttc ttctacttct
    24361 tgtttcattg cttcggtatt agtccataat cgctctgacc aaacgacgcc atgaaattgt
    24421 gtatcaacga ttgtatctat aacttcttta gctacttgta caccttcacc taaaatacct
    24481 gcttgatcac tgaacacacg ataagctgtt gattcgaaat attccctcat cgataattct
    24541 gtttgagctg ttgcataagc aattaagaat tctatttgaa tctttaacat ctgttctcta
    24601 gatacataca tcttagtgtt atacttcttt aattcttcat ttgctctatc gctaaagtcc
    24661 ttgttttcga ccaatctttt tgcttcttct tgaaacgctt ttacatcgaa ctcatcaata
    24721 atcttttgtg cttcttgtaa tgtaacgcct gcaaaatctc cgtacttaac aataaacgca
    24781 ttgatctctt tttcaatgcg cttaatcatc atattcaata tacgttctat ttcttcagct
    24841 ttagttttat cacgcttcaa ctcattctcg attgctttgc gtccgcgttc ttcccaatat
    24901 tcttgagtgt ttttgttagg caattacaat cattcctttt tatcaacagt atcttttgta
    24961 tcatcatctt gttcgtcatc attgatgtct ctagggtctt tataaatacc tttttgagct
    25021 tttttaatag attctttctc atcttcttct attttcttga cttccaattc agggtcttgg
    25081 aagaacgaga atagagacat caaagttgtt tgactaatct tcccgccaga atcaatataa
    25141 gcttttaatt cctcgattaa tgatttaggt aagtttctgt tgtatacgta tctaacagta
    25201 ttgaaatctt tgttagcgtc aatcgaccgt gtatttttaa gtattgtctc taacaactta
    25261 gcacgacgtc ttaacccttt agtaaacaat ccttctttag ttttagtacg ttgttctaat
    25321 ccgaataatt tgtatttcat agcctcgccc gattgagtac cgctgaagtt atcatctttc
    25381 atattaggcg tattggtaaa catgtgtata tcactgttta aacggtcttt ataagcttct
    25441 gtaccttgta catcgtattg tttataaata taaccgccat caacagagcc ttctgtttct
    25501 ctaccttcgc tatcagcgta aacagtcggt tctaaaaaca aaacattagc ttccttttgc
    25561 tttctaactt ctacgggatc taaatttaaa ttacctttaa taagtaacat agcgtcattt
    25621 aaatcactca tatagttagc agtatctgat tcagcattat catacaaatc aattaaagtg
    25681 attactttct cataatcccc ttttcttctt tcgttgttgc taaattctgt aataggcata
    25741 cgttcgaaag agtgtgattc aaaaccgttt tcacgtggtg tgagcttcaa tccatttgtt
    25801 ctactggtaa gatatctata aacaccgtga gaagtaaata aatcaactgt aaacacttca
    25861 tcttcgtcag tcttgtctat tggtttagtt cttaaatatc taacgcctgc gatactatta
    25921 cgttcaactg tgttgtcata tatgacaaaa gtactcattg catcactctt gtataaacgc
    25981 gtttcatcat cttgatttct aatcatcaac tcataagctt tgccgtaaat tgataagtct
    26041 aatcctagag atctattgtg cgactcaaca tcattcaaat cattgaacgc ttcaatagct
    26101 tctaatactt ctttgtcatc atcttgatat tgaattggat tacccaagaa ataaccattg
    26161 atgaaatcac taatataaga tgcgtaatca tgggcaacac ggttgtctga catgtactct
    26221 tctttgcgtc gtgttaactc aactagattc ttagttttac cttcgtaata atcacttaac
    26281 acttttaatc taggacgttg gtaatccatg tgatgtttaa tgtatttact cacttcatta
    26341 acgttttgca ataaatcaga ttctgtcccg tcatacgtgt aaacaacatt agcttcatca
    26401 ttaaataagt agtttctgtt ttcccgtaga tctgtatccg tttcaaattc gtttactttt
    26461 aacatttgtt ccctcctata atcctagaga tttaattact tttgttttgc tttctatatt
    26521 ctttttacgt tttttacgta cgatatgata tttctcaaga ctataacgca atgcatcgat
    26581 aatatggtta ttagcatcta taggcttgtt caaccactta ccgtcattat cttggtcaaa
    26641 tgtataagtg ttgaactctt caatagcatg ttcacatgat ggatgtataa taacttcaaa
    26701 gccttgaatg aattgaatgc ctggtaaaat agtattagcg cctttcaatg cttttcttat
    26761 acctttaatc cctttagatt tcaattcact gatcactcta tctccgccag caccataatc
    26821 ggctgcaata tctacatcat ctaatccttt tttaataagc atttgtttta tatcatcagt
    26881 tagcatcgct ttttcatagt gttcatcata gatgaataac tttttgtttt ttaaatcgac
    26941 aaccgtacta acaactgttg tagggtcttg actaaatcca aaatccattc catgagttat
    27001 ttcttgagtt cttttaaact ccacaaacca atcaaagtct tccactttaa aattatcgaa
    27061 tacaagccct tctgcaacgc cccaatctcc atcgcaaacg attcttgcac gtctaggatt
    27121 ctttatatac aaatcttcat atcgttcaat atcaactttg tctagccatt cattaactct
    27181 ataagttgtt gtatctgaaa aagtgttgtt taattttgtt tcttcatcaa aaaatgtagg
    27241 cttcaaccaa tgtctttccg accacgggtt aaaagtgact gtgatttgct tgaaaaattc
    27301 cggactatcg tagctaccac gtattgactc aacaacagtg ctaaacttag cgaatgtttc
    27361 tatttgataa gcctcttcaa accaagccca acacaaaatg cctgtatcaa cagtaatcga
    27421 tgttattttc aatgggtcgt ctaaacctct aaacagtatt ttttgtccag taggtttata
    27481 cgttatttcc ggcaaacttt cgttgaattt aaataagtga gcaacgccta attggttagt
    27541 tgcccacttt aaatctgtat acgttgattg tttgttagtg ttgctaaatc ttctgactac
    27601 aagtatattt gcccaatcat atttcattat tcgataaatg agattaatag cggtagtttt
    27661 acttttcttg ctaccccttg aacctttaac aacgcggtaa aagtttttat tgtgccaaaa
    27721 cttattgtag ccaccaccga ttttattttt tagatcaagt atttcataca tgactaatca
    27781 tcttccggaa tattatcaac aaacatcggt attttgtggt cgacttcttg tttgtctgta
    27841 aataatttgt gatgtctacc taacatctct aaggctttgt tttggtcact tattttaggt
    27901 gacttagtaa caagttgtat gtgttcatcg tatactaatt gcattttgcc agtatccgga
    27961 ttctctttat agtctccagt ttttgttacg acagcttcaa cttccgtgtg ttcacctcta
    28021 gccgttctag ttaacctata caacacttct ttacctgaca taatattctc atcaaagagt
    28081 tttgtttcaa cctccttgat ataattctga atttcaacat tcttcaacat acgctgacct
    28141 tgtgagtacg ccgtcttttc gctatatcca gcatgcacag ctgacttagt agcattgcca
    28201 taacattcag taccgggtat tgtatatact tctacaaaca aacgttgctt tttagttaat
    28261 ttgttcattt catttaccac caactctcgc gctatacgct ttttaaaatt aaaaaaggga
    28321 ttggctataa tcagccaacc cacatagatc ctttattcct aattgcgata agggaaacgc
    28381 agtaagatag tcaatatctt acgctatcat attaacaccg aaagtgacgt tatttttcca
    28441 gactttttcc aaacttaatg tattatacct aattcatcag ctaacctaac taatatatct
    28501 ttcctcatat cataagcggt agatttactt acatttattt cttgagctac accagttaaa
    28561 tttaatgttc taggcttttt aaaataataa agttccataa gtttttgagt ttctgtagtg
    28621 ctatgattat atacaacctc tatagccgat ttcattctgg ccaattgcga taatcttcta
    28681 tcattaacaa ctctaatagc ttttatttca gttacactta cattgctttg caccctatct
    28741 ccaccgatat tagtatcttg ttgactccac gggtttaaaa cttcatctct tacacgcgct
    28801 atatctttat cgaagtaatt gtaattgctt aattcacttt ctaaatatct ttgcgttgat
    28861 tttctcaaac tcatttgttt aacccccgtt aaccttcaaa atgtctcaat ctacttctta
    28921 atatctctat ctcccgctct ttaactttca catcgccttt taactgttca gcttgcaaca
    28981 tcacaccaaa caataagatg actagtaata taattgctat gactaaccac atcatctact
    29041 ctgacacctc cgccctcatc aaatcagact gatcgctcaa ctttgcgaag tcactcggca
    29101 tctctacatc attattagcc gtcatcataa tatatacttg ctcagttaca tacttaccta
    29161 gctcatacat tgctagtaag aataatagtc ttaatatttg tttaatcatt gtttatctac
    29221 cttctttact tcgtataaga ccggatataa atttaaaaag tgtattctat atccaatcgt
    29281 cttaacttct actttgtcgc ctacttttaa cctagcttgt atgtctgcgc tatcaaattt
    29341 ctttttgaat aataaatcag aattttcaat gacttgttta tcatctaata caatatagaa
    29401 tttgtcctct ttatcttgtc ttttgttata tttatctgta attgtccctt gatgtacttc
    29461 tttgttttgg taactagcca ctgtatagat aggcaatgcg acaacaagta gcaatgcggt
    29521 tataccgaat aatgacagta ttccaacaat aaagatgtcg aacccatcca tatttttaag
    29581 ttttttaatc atttcccaca ctcccttata ttttcaaaca actgacccac tttaataact
    29641 gcatcccttt taacttgttt ctcgtacttc tctttcgctt cttctttact ctctgcctca
    29701 acaactgtaa acctttgatt actcttagct ttagttatgt gtgtatgttt acgtcctgtt
    29761 gaatctttga atgttgtgac taagtattgt gtcacttccc caaaacctcc ttgacccgat
    29821 ctaatatgtc tttatacgta tcctttccct gcgtctgctg ttccatcttg tctttcgtgg
    29881 ttccttttca ttttcttttt gtatgcgtca atgagttggt cgatagtgta gtagttgttc
    29941 gctaatgcaa acggtaaaaa taagttgcta ctatatggac tttcatacat ttcatctata
    30001 gttgacataa attcatctac tacatcacta tcgttaaaat cgatttcaac tcgttctata
    30061 tagtcgttaa aatctccgtc atctaaataa cccaaaattt cttccatgtt atctgcttgt
    30121 tgattagcaa tactcaatcc aaacgctaac atgtctgcta actcgtctag ctgtacgtct
    30181 aacggtttac ctggtttctt cttccaattc ttgaacgttt ccaatgtgtt aaaccattca
    30241 aagaattcaa ccacatatgc tattttgcta tctcgtaagt tcagcgtcgg tattctatcg
    30301 tcgaactcct tttgtatttg taaaagttct tttaattgat ctactgttaa attattcatt
    30361 tattcgttat ctcctatcgt tttaattcct caataaattt aagcactcta tcaatatcaa
    30421 tctgttcact ttctgacttg cgtttattca accaataatc taactcgtac caccagtcgt
    30481 cgtttaaata cttttcttct agcaatgcat cacgttggtc gatgatttca agcatttact
    30541 cgtcctccaa aaattcaaaa tatctttcaa tctcatgctc gttagcaaag taatatctag
    30601 gcgtcttatg agatttagga ttaatccaaa taatgtaaat aggtatcctt tgaaatgata
    30661 aaaatccagt taataacact tcttcattaa aaatttcaaa gaaacctaat tctttttgaa
    30721 cctctttcac attatctttt gttacgtata ccgactttaa gtttttattt ttgataactg
    30781 ggtgcggcac cacatttttt aatcgtttcc attccactca ctcgtccccc ttaattagat
    30841 aaattggttt agtaataaaa tctataatgc taataactga atcatcagac agtttataat
    30901 gtgtatctct aatatctccg accaattgca caatctctag actttcgttt gtttcatggt
    30961 tatatacttt atctcctaca ctaatactca ttttcctgct cctcctcata tttatagacc
    31021 acttgccccg tcataatccc tactgcttca tcaagaccaa tatcttcttt gagtgcatct
    31081 tgcatagcat taggtaaacc ctcaagtatt tcatcaaacg cttgcgcttt cttatacacg
    31141 tcttcaacct cttttagtaa tccctctgtg tcattaccgt tataggcact agcactgata
    31201 attgattgtt caatttgttc gcggttattc atcatttcca tctcctctaa aataaagtta
    31261 gttgcttctg ctcctcgtat tccaaaccat gttgctttat atatgtttcg agctcttctg
    31321 ctgtatcaaa catcttttta acgccttgcc aacctggtac gatatgccca tgaaagtaat
    31381 aagtgccgtt tactacatga atatgtgcca ctcgttcgtt atcctgatac agatatctct
    31441 tagatccaaa gaattgattt aggtattctt tgcgtgcgtt atctgtcatg atctacttct
    31501 taactttcac gaatatgtcg ttttccatca ggtagcacgc ataacgtcct cttggatgtt
    31561 tctgtggtac attaaacaaa tgtggcttct ttcttcttag ctcagcctct ttcttttgct
    31621 ttctttccaa tttgcgttcg agtctagctt gttccagtct ttctattgtt ttcttttctc
    31681 tgtactcgct taaacgcgta ccttctggtg cgtccattgc ttcatgtagt tcccaaccgt
    31741 cttttactct cttagaaacc attccagcgg ttataccgtg actttctatt aattccattt
    31801 caaatttact gaacctataa ggtttatcgt ttattgttac aattcttgct tttctcgcca
    31861 ttttatccac ctcttatatt tcttctattc gtatgattat tttgggctca attccataac
    31921 gctttgagct agttatttct gcaatttgat tgtcatcttt ccacaaataa ttgttacaag
    31981 cgtctagaaa agtaaaactc taattcaatt tttaatgcat tttctagatt tagctttggc
    32041 atttgatttt gtaaatattt tttatgttct gtatattttg taggcatata tgtgtgtgca
    32101 tatctacctt ttgtgctaaa acgcggtcga ggcgagccca taggtgcctc gaaagtttcg
    32161 ttaaatttaa tttctatctc catgtaatcc ctcatatata ttcaaataag cttgtttggt
    32221 gtcctaactc catttgttca ttatcaataa gtgtttttaa ttcataatca tctaagtacc
    32281 aacgtcgacc attgaatttt gtgtgtttta atccaacaac taaatgccgt acatctttaa
    32341 aatgtggtgt aactgaaaac attttgttgc cgtcatgatc aaatagatag tatttatcaa
    32401 atgcatccat tttcaatcac tcccatttgc tatttagacg cttaataaaa gcttctctgt
    32461 ctttctcaag gttttcatct acttccggcg ttttcgtttc tctcgtgctg tctgtgagcc
    32521 atttgggtgt tttttctttt gattgtttaa cgaaaggttt ataattttgt tttttgcttt
    32581 caagttgttg cttttcaaat gcacgtactt gttcaataga tttcaagttt gcattaagcc
    32641 atgtattcaa aatgctttta gcatatcccc aagtaacttt atttctgtct ttagcgattt
    32701 taagtgatgc ggtaactatt tgatctgaat cattttcaaa tgaatcaaga taataattta
    32761 aatcgtctaa attgtaagga gttatgaaac cgaatccgtt atcttggaag aagtcgaagg
    32821 cggttacctt cttcttctca ttctcaccat tctttacatt atccccattc tttacattct
    32881 tgtttgtgtt gatttgttgt ccatttgttg ttgatttgtt gttgatttgt tgtccatttg
    32941 ttgttgattt gttgtcgttt ttgctgtcgg aattttcttc catactttga taaatcgccc
    33001 aattgacaac ggttataaca gaaaatttgt tgtcggactt tacgacgata gttccaaggt
    33061 tttctaaaag ctttatgtag tctcttactg tggattcttt gagacgtaac tcttcgcttg
    33121 ctcgctttct cccgaacaca aattgacctt tttctaattc gacaacccgc ctgccaacaa
    33181 gctgtgtatg atccttatga ctagctttca taagacaata tgcaaatact ttgaataact
    33241 tttcgttctg aaaaataggc gaatctaata gttttctatg aagttttatc caaccagtca
    33301 tatacacacc tcactttcaa accggttaaa ttagaatggt aaatcattgt catctatttc
    33361 aatcggaaca tttgcattcg caaacggatt atcttttact ggtttgttat ttgaatattg
    33421 cgattgtcca cgtgtttgtt gtacttgttg ttggtataaa tcttgttgag tgtcatttga
    33481 gtttttcggt tctaaaaatt gaatactatc agcaataact tccgtaacat atacacgttg
    33541 accttcctta ttttcataat tccgcgtttg taacctacca tctacgcccg tcaacgatcc
    33601 tttagatagg tatttattaa cgttctctgc ttgtttttta aatacgatga tattaataaa
    33661 gtctgcctcg cgctctcctt gtgcattcgt aaatgtgcgg ttaactgcta atgtgaatga
    33721 tgctacattt acaccacttt gagtggttct taattctggg tctctagtta aacgaccaac
    33781 taatattgtt ctgtttagca tttataaacc tccaacataa acgggcgcgc ccgtcacttt
    33841 ttgtatttca cttttaatgt attttgcatt tgaattttga ctacttaaat gaattaaatg
    33901 tatttcttcg agtctagtta aatcatttgc ttttaacatt ccgatagcat gttctaagct
    33961 aaaatgagac tccataattc tgtttgctaa tgtgctgtgc acactgccgt tttttatgtt
    34021 ttcttgcatt tgttcataga tataattaac ttctagcatc atgtgcgtaa tgccattaaa
    34081 tttgtatttc aaatactttg tatcagtaac atacagaacc ttataaccta gtgtgctttg
    34141 taataagaaa gccacaggct cgttagcatc atgttcgatg tcaaacggta gaattgacca
    34201 tgtacctatt cgcagctctt gctttgcctt aatcgtgcat aagcgatgac tttcaaaatt
    34261 catagcttgt tgtgttccag cagtcatata gcttattaca ccattgtcga caaactgctt
    34321 tgt
//
LOCUS       DAMZLE010000013        33045 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMZLE010000013 DAMZLE010000000
VERSION     DAMZLE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33045
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.013"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..409)
                     /locus_tag="POB92_000633"
     CDS             complement(<1..409)
                     /locus_tag="POB92_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDI6578153.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKD"
     gene            complement(406..1452)
                     /locus_tag="POB92_000634"
     CDS             complement(406..1452)
                     /locus_tag="POB92_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI6578154.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAASNHIIKGCEL"
     gene            complement(1536..2372)
                     /gene="prmC"
                     /locus_tag="POB92_000635"
     CDS             complement(1536..2372)
                     /gene="prmC"
                     /locus_tag="POB92_000635"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI6578155.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEDIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(2359..3435)
                     /gene="prfA"
                     /locus_tag="POB92_000636"
     CDS             complement(2359..3435)
                     /gene="prfA"
                     /locus_tag="POB92_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI6578156.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(3436..4035)
                     /locus_tag="POB92_000637"
     CDS             complement(3436..4035)
                     /locus_tag="POB92_000637"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI6578157.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(4382..4636)
                     /locus_tag="POB92_000638"
     CDS             complement(4382..4636)
                     /locus_tag="POB92_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI6578158.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(4754..6070)
                     /gene="rho"
                     /locus_tag="POB92_000639"
     CDS             complement(4754..6070)
                     /gene="rho"
                     /locus_tag="POB92_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI6578159.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIEIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(6317..7744)
                     /locus_tag="POB92_000640"
     CDS             complement(6317..7744)
                     /locus_tag="POB92_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI6578160.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPKKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(7982..8317)
                     /locus_tag="POB92_000641"
     CDS             complement(7982..8317)
                     /locus_tag="POB92_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6578161.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(8406..9665)
                     /locus_tag="POB92_000642"
     CDS             complement(8406..9665)
                     /locus_tag="POB92_000642"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6578162.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGMELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(10131..10991)
                     /locus_tag="POB92_000643"
     CDS             complement(10131..10991)
                     /locus_tag="POB92_000643"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDI6578163.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            11209..11730
                     /locus_tag="POB92_000644"
     CDS             11209..11730
                     /locus_tag="POB92_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDI6578164.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(11839..13449)
                     /locus_tag="POB92_000645"
     CDS             complement(11839..13449)
                     /locus_tag="POB92_000645"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDI6578165.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLNQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(13785..14315)
                     /locus_tag="POB92_000646"
     CDS             complement(13785..14315)
                     /locus_tag="POB92_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDI6578166.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(14427..15287)
                     /locus_tag="POB92_000647"
     CDS             complement(14427..15287)
                     /locus_tag="POB92_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6578167.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            15711..16514
                     /gene="coaW"
                     /locus_tag="POB92_000648"
     CDS             15711..16514
                     /gene="coaW"
                     /locus_tag="POB92_000648"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDI6578168.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(16644..17315)
                     /locus_tag="POB92_000649"
     CDS             complement(16644..17315)
                     /locus_tag="POB92_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDI6578169.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(17714..18907)
                     /locus_tag="POB92_000650"
     CDS             complement(17714..18907)
                     /locus_tag="POB92_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDI6578170.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(18907..20091)
                     /locus_tag="POB92_000651"
     CDS             complement(18907..20091)
                     /locus_tag="POB92_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDI6578171.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            20402..20872
                     /locus_tag="POB92_000652"
     CDS             20402..20872
                     /locus_tag="POB92_000652"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI6578172.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(21024..21374)
                     /locus_tag="POB92_000653"
     CDS             complement(21024..21374)
                     /locus_tag="POB92_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578173.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCFPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(21388..22689)
                     /locus_tag="POB92_000654"
     CDS             complement(21388..22689)
                     /locus_tag="POB92_000654"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDI6578174.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIIIE"
     gene            complement(22970..23632)
                     /gene="deoC"
                     /locus_tag="POB92_000655"
     CDS             complement(22970..23632)
                     /gene="deoC"
                     /locus_tag="POB92_000655"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6578175.1"
                     /translation="MHSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            23947..24657
                     /gene="deoD"
                     /locus_tag="POB92_000656"
     CDS             23947..24657
                     /gene="deoD"
                     /locus_tag="POB92_000656"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6578176.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(24778..25221)
                     /locus_tag="POB92_000657"
     CDS             complement(24778..25221)
                     /locus_tag="POB92_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDI6578177.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(25421..25834)
                     /locus_tag="POB92_000658"
     CDS             complement(25421..25834)
                     /locus_tag="POB92_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDI6578178.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDCIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(26292..27665)
                     /locus_tag="POB92_000659"
     CDS             complement(26292..27665)
                     /locus_tag="POB92_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDI6578179.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(28337..28567)
                     /locus_tag="POB92_000660"
     CDS             complement(28337..28567)
                     /locus_tag="POB92_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578180.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(28598..29536)
                     /locus_tag="POB92_000661"
     CDS             complement(28598..29536)
                     /locus_tag="POB92_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDI6578181.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(29972..30631)
                     /locus_tag="POB92_000662"
     CDS             complement(29972..30631)
                     /locus_tag="POB92_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6578182.1"
                     /translation="MLVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQGMK
                     AILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKEAN
                     IKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAAGS
                     GKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEIEK
                     D"
     gene            30789..31109
                     /gene="czrA"
                     /locus_tag="POB92_000663"
     CDS             30789..31109
                     /gene="czrA"
                     /locus_tag="POB92_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDI6578183.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            31111..32091
                     /gene="czrB"
                     /locus_tag="POB92_000664"
     CDS             31111..32091
                     /gene="czrB"
                     /locus_tag="POB92_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDI6578184.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHAH"
     gene            32357..>33045
                     /locus_tag="POB92_000665"
     CDS             32357..>33045
                     /locus_tag="POB92_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6578185.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPVFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFK"
BASE COUNT        10256 a         6307 c         4618 g        11864 t
ORIGIN      
        1 tatcttttaa ttttaaaatt aaacttacaa gttcttcata ggtatgtaca tacatttctt
       61 ttgttccacc gtatggatct ataacttctc ctgcttcttt tacatattca tgcaatgtga
      121 aaacatgatt ttgcaaacca aagtgtgcct ctattaattc tttgtgcgaa tacgacatcg
      181 tcaaaataat atctgctttc aaatctgctt cagtaaattg ttgcgataag gtcgtttcag
      241 ctaaatgatg ttcttcaact aagtcttcaa cataattcga aacaccttga ttgttcacag
      301 cgaatatacc tcttgattca aattgatgat ttggcataac ctcttttgca atactttccg
      361 ctaatgggct acgacatgtg ttacctgtac aaacgaataa aatcttcata gttcacatcc
      421 tttaataatg tgattacttg cagcttttaa catgcgattc ataattgctt ctgtattatc
      481 attcagctca aagccgtata tatacgccgc tgaaatattt tcattttcat caagtgaatg
      541 taacacatca taaagattat gacttgcttg tttaacatca ttgtcatcct gacataattg
      601 aatgaattgc gcttcacttg gtataaacgc caccttatta ctcggcacaa taaaagctat
      661 agaagaccaa tctttaccgt catttccaat tttgctctca atatctgtaa taattgtaag
      721 tggtgtattg ggtgagtaat gcttatactt catacctggt gcaattggct gttcagtatc
      781 attataatca gcatgggcga tactattcgg aagtatttct gtaatcattg ctgctgttat
      841 agaaccaggt cttgcaattt tataaggaaa agatgtgcaa tctaaaaccg tactttctaa
      901 tccttcttca ctttgttcag cttgaacaat accatcgata cggccattca aatcttgata
      961 tacatgattg aaagttgttg gtgaaggtct accacttaaa ttagcacttg gagcagctag
     1021 aggttcattt atgatttgta ataattgtct acctacagaa tggcttggca ttctaacagc
     1081 aactgatgat aaacctccag aaacttttcg acatagatag cctggcttta acggcaatat
     1141 aaacgaaata gggcccggcc agaatgcctg cattaacttt tctacgcgtg gatccaaagt
     1201 atatgtaaaa tcttttaatt gacctttact gtgtatatga acaataagcg gattgtcaga
     1261 tggacggcct ttagcttcat atattttagc tacagcttct tcatctgtcg catttgctgc
     1321 aagtccataa actgtttcag ttggtaaacc tattaaacca ccgtttaaaa caatgtcttt
     1381 tatttcatta attttaggat attgctgtaa atcttcatta tattctctaa catcccaaat
     1441 tttagtatcc aacttaatca cgcctttctt atttatcata atataaagca aaaagctatg
     1501 cacttaacta atcatagcaa aggcataact tctaattacc atttaaatga gacgattcga
     1561 tcgtggccat ttatatcttt aataatgtcg atttttttgt caggaaattt atttaaaatt
     1621 attgatttaa gtgcctcacc ttgattgtaa ccaatttcaa aaacaactgg gctgcctttt
     1681 tccataacgt gaggtaaatc ttcaatgatt gattcataaa tagcatatcc atggttatct
     1741 gcaaacaatg cctgatgtgg ttcgaatctc gtaaccgttg gagacatcgt aaccatatct
     1801 ttttcatcta tatatggtgg attagatatc aagccgttca acttgatatc ttcattaatt
     1861 aagggcttta atgcatcccc tgttaaaaat tgtatttgtg attgatgctt ctcagcatta
     1921 ttacgagcca tattcattgc ttcaagtgaa atatcagtag caataacatt taaatccggc
     1981 ttttcacatt tcaaagtaat tgcaagtaca ccactacccg ttccgatatc tacgattgtt
     2041 gcatcatctt ctaactgttg taagaaatgc aacattactt cttcagtttc aggtcttggt
     2101 atcaaacaat ttgagtttac atcaaacgtt ctaccataaa atgaggcaaa gccaactata
     2161 tactgtatag gctctcctaa taacatacgt tgtaatgcta agtcgaactt cataatcatc
     2221 gctttcggca tatcatcatg catgtggact acaaagtccg tacgcgtcca ttgaaataca
     2281 tctaacatta accattcagc tcgtgtttgt tcaaaccctt tttgttgtgt taaatgaatt
     2341 gcttcatcta acttttcttt ataattcacc attattaagt tctttcaatt tatctgtctg
     2401 ctctgataaa gtcagtgcat ctataatttc ttctaaatgg ccttccataa tttgccctaa
     2461 tttttgaagc gttagaccta tacgatggtc tgttacacgg ctttgtggat aattataagt
     2521 tcgaatacgt tctgaacgat caccagtacc gactgctgat ttacgttgtg acgcatactt
     2581 ttgttgttct tcttgaactt tcatatcgta taaacgtgct tttaacactt tcattgcttt
     2641 ttcacggttt tgaatttgag acttctcaga agatgttgca atgacaccag ttggtaaatg
     2701 ggtaatacgt actgcagagt cagttgtgtt tacgtgctga ccacctgcac cacttgaacg
     2761 atacgtgtcg atttttaaat cttcatttct aatttcaatt tctacatctt caacttctgg
     2821 taaaactgcc actgtagctg ttgaagtatg aatacgtcca cctgattctg tttcaggcac
     2881 acgttgaacg cggtgcgcac cattttcaaa tttcaattta ctatacgcgc cattaccaga
     2941 aactgagaaa ctaatttctt tgtaaccacc atggtcactt tcagacgctt ctactatttc
     3001 agttttgaat ccttgtgatt cagcatactt tgaatacata cgcattaaat caccagcaaa
     3061 aatcgcagcc tcatcaccac ctgctgctgc tcttatttct acaataacgt ctttgtcatc
     3121 attaggatct ttaggaatca ataatatttt aagctcttct tcaagatttg gaagttcagc
     3181 tttaatacca ttactctcct cttttaacat ttctacttct tctttatcat cagtctcact
     3241 taacatttct tcaatatcag ctaattcttc ttttttagct ttatagttac gataaacatc
     3301 tacagttttt tgtaaatcag cttgctcttt agaatattta cgtaatttat ctgaatcatt
     3361 tacaacatct gggtcactta acagttcatt taactgttcg tatctttctt ctacaatatc
     3421 taattgatca aacacttata attcctcctt attattatca ctaggtgcta cgatatggtg
     3481 cgcgcgacaa cgtggctcat aactttcatt ggcacctact aagataatcg gatcatcgat
     3541 tttagctggt ttaccattta ttaatcgttg cgttctacta gatgaagaac cacaaacagc
     3601 acaaactgct tgaagtttcg ttacttgttc actgacagcc atcaatttag gcattggttc
     3661 gaacggttcg cccctaaaat ccatatctaa tccagcaaca ataacacggt gtccatctgc
     3721 tgatagtttt tctactatac ttacaatttc atcgtcaaaa aattgcactt cgtctattcc
     3781 tataacatca acattagtta agtcgtgcgt cataatttca cttgctttag aaatattaat
     3841 cgcttcaatg gcattaccat tatgagagac cactttttct ttatgatatc gatcatcaat
     3901 cgccggttta aatacaacga ctttttgttt agcgtatata ccccttctta gacgtcttat
     3961 tagttcttcg gatttaccgc taaacatact acctgtaata cattctatcc aaccggaatg
     4021 gtaagtttca tacattgaga gttccacctt tttcaaaaca taatcgcttt attatatcat
     4081 atttcaaata ttcataaatg tctttttcat aattatatcg atattgtaca tgaacaatta
     4141 ttttataaat ttgtcttgca tatttatata cagctattct aatttgttgc ttcttattcg
     4201 tgcggtgttt ctactcaata caaactttaa taaaggggga aatttatcat tatgtattaa
     4261 caacatggta ttgagtcact tctttgaaca cagtcataag tatataccct tactacgctt
     4321 ataaataaaa aagctgtcta caattccatt ataggatgca gacagcttaa cgattactat
     4381 attagttgtt tgatttaaga ccaaactttt tgttgaaacg ttccacacgt ccatccgcag
     4441 cagcgaattt ttgacgtcca gtgtagaatg gatgtgaatc agatgaaata tctaaacgaa
     4501 taactgggta ttcttttcca tcttcccact ccatcatttc tgaagatgtt tttgtagaac
     4561 cgcttaagaa tttgaagttt gtagtagtat ctaaaaagat tacttggtga tactcaggat
     4621 gaattccttg tttcattatt ttcagctcct ttgccctgaa ccatctggaa cagagttatt
     4681 tgtgagtttt tacccaatac tgtgtaatta taaaggtaat taattcaaaa cgcaagcccc
     4741 tatataatgt ttattaaatt ataggtcgac ccgttttagt actttcttct gcagactttt
     4801 gtagctgctt gaagaaatct tcattattct tagacctttt aagtttgcga ataaatcttt
     4861 cagtaaagtc agttgagtca gtgaatagat ttcttaattg ccataatgtg tctaattcag
     4921 atttacttat caacaattct tctttacgcg ttgaacttct gccaatttca attgcaggga
     4981 agatacgacg ttcagacaat ttacgatcta aatgtaactc catgttacct gttcctttaa
     5041 attcttcgta aatcatatcg tccatacgtg aacccgtatc aactaatgca gttgcaagta
     5101 ttgttaaact tccacccgct tcaatatttc tcgctgcacc gaagaatgct tttggtttgt
     5161 gtaaagatgc aggatctaaa ccacctgata atgtacgacc acttggtgga ataactaagt
     5221 tataagcgcg tgctaatctc gttatagaat ccattaaaat aatgacatct tccccaattt
     5281 ctactaaacg ctttgcacgt tcaagtaata attcagctac tttaacatgg tgttctggtg
     5341 gttcgtcaaa cgttgaatga acgacttcag cagcttctac tgagcgttct aaatctgtta
     5401 cctcttcagg acgctcgcca actaacaaaa taaatagctt tgcatctggt ttgttcgtac
     5461 tgattgcatt cgctatttct tttaataacg atgttttacc tgctttaggt ggcgccacta
     5521 ttaaaccacg ttgacctaaa ccaatcggtg ttactaaatc catgatgcgc gttgaataat
     5581 tttgtgtttc tgtctctaat ttaatacgct catctggata aagtggtgtc aaagcttgga
     5641 aatgcggacg tttcttcact tcttctgcgt tatggtcatt gacaaagtca acttgtaata
     5701 agccataata tttttcgtta tctttaggtt ttctaacttt cccagttact ttatccccac
     5761 gtttaatttc aaaacgacga atttggctag cagatatata aatatctttt tcccctttag
     5821 aatagttcac tgttcttaaa aaaccataac catctggttg tatatcatct aagatacctt
     5881 ccatataata gttaccatct ttttccattt gtgcttccat aatagctaga acaagttctt
     5941 ttttatttaa tttactatag ttcgtcaact taagagtttt agctttttga gtgagttcct
     6001 tggtagtata gttcttgtac aattcgtgga atgattcata ctgaggagat gtacgttctc
     6061 tttcaggcat atagtttaca cccatttcat tattttttag ttagtaagta atagataggc
     6121 atgacaatgc ataaataact gtgctagcac aatcccatta tgctaatccc caacctttta
     6181 aatacatgaa taactatagc aaatttttat tttaattaca aaatctattt ataaaaattg
     6241 caaaattaaa acagtacaca aaacttaatt agtcatgtgc actgttttat tgcgattttt
     6301 aattcaaaaa tcaaatttat ttaaaatatc cagctataga tttcacttct aagaactctt
     6361 caataccata atcgccccat tcacgaccta aaccagattg tttatagcca ccaaatggta
     6421 aatctggctt tctacctgct tcgtttattt ctactgtacc tgcttcaata gaacgagcta
     6481 ctttatgcaa tgtttctttg tccttaccaa taacatatcc tgccaaacca tattttgtat
     6541 catttgcaat ttgaatcgct tcatctaaat cgttataagt gataactgac attactggcc
     6601 caaaaatttc ttcttgtgct atcgtcattt gattatccac attaataaaa attgtcggac
     6661 gtgcaaagta tcctttttca agtccttctg gtttaccagg accaccataa aataattcag
     6721 caccttcttc aataccttta ttaatataat tttgtacttg atcaaattgt tttttactaa
     6781 tgatagggcc tacttgtgta ccatcttctc ttggattacc gacacgcact tggctaaatt
     6841 gttcttttaa ttcagctaag aatgcatctt taattttttt aggcactaaa actcgtgtac
     6901 cagctgtaca tacttgacca gtattattaa caactttgcc tgttgttgct ttagccgctt
     6961 ctttaatatc tacgtcatct aggacgatgt atggtgattt gccaccaagc tctaatgata
     7021 cctttttaaa atctttagca gctttttcca taattttaga accagtaggg cctgatcctg
     7081 taaatgacat catgcgtact ttaggatgct cagataaagg attcccaacg ccagcaccat
     7141 caccattaac aaggttaaat acacctttag gaacaccgac tttatcaaaa atctcagcta
     7201 aaataacagc tgcaaatggt gtttcttcag atggtttaag tacaactgga ctaccagccg
     7261 caaatgctgc tgctaatttt aatgatgttt ggtttgtagg gaagttccac ggtgtaatta
     7321 agccagatac accgattgct tctttaacaa ctaaatcatc tccgcggcgt tcttcaaatt
     7381 cgtagttatc taatgcgtct ctcgctgcaa caaaatggtt tagtcccatt tgataatgga
     7441 cacgctcaga taatgataaa ggagcaccta attcatccgt aatagcttgt acaatatcgt
     7501 cttttctgtt ttcatattct tttacaattt tatctaataa cgcttgtctt tctttcacag
     7561 atgtatgacg gaactctaaa taaacattgt ctgccgcctc gacagcttta tcaacatcag
     7621 ctttattgcc tttagcaact ttcccgatta cttcttcggt tgctggattt ataacttcta
     7681 tcgtttcatt actattactt tctacccatt cgccattaat gtattgcttt gtgtagtctc
     7741 tcattattct ttcactcctc aagaatgatt tatatattat ttatccgtaa tgcatacgat
     7801 ataaacatta cattccatta tgatacgctc ccttttaata tgttcaaaat aaaatgcttt
     7861 aaaattttgc tgcatctatt tttttatatt tttaaataaa aataactatt cccacatcat
     7921 cgcaaataat gcgaagactt agaaatagtt ataatcaatg ctatgtttaa acgtcatgta
     7981 attatttagc agtacgttga tctgttaaat cgacatatga ttgcgcccat gcttcaattg
     8041 gatgtaaagc ctctgctaac gctttacctt tttcagttag cacataaata atttgtactg
     8101 gactcgtaga aatgatttgc ttctcaacta actcccattg tgcaagctct gacaacttaa
     8161 gacttaaagc acgtggtgtt attgttttca aatctctttt catatcggaa aagtgtgctg
     8221 aacagtcatt acatcttgag agataattaa tgattaatcc attccaactt ctaccaagta
     8281 ttttaaaagt ttcttcgaga tacggacata cttccatcat cttcacctct catcaataaa
     8341 tttagaatgt aaacgaacac ccttttttaa tcagtataat tattatacca caaaacaaga
     8401 taaatctata cagtttccgt ccaaatatct gcacctaaag cttttaagtg ttctacaata
     8461 tctgtataac ctctataaat atgttttaca ttgtaaattg tagttacacc ctcagcaatt
     8521 aaaccagcaa taattaaaca agctcctgct cttaaatcgc tagcataaac ttcagcacca
     8581 tgtaatgttg atggctttat cgttgctgtg ccttcgtcaa cttcaatatt tgcacccatg
     8641 cgctttaatt cttcaacatg tttaaaacgc tccggataaa tcgtatcagt tacaaatgaa
     8701 ggaccatttg ccataaataa taatggtgta ataggctgtt gcaaatcagt agcaaaacct
     8761 ggatatacta gtgttttaat atcaacaaat tgatatggcg cattattatt gatgcgaatt
     8821 ctttcgtctc ttacatcaac attcacacct aattcactaa atttagcagt taatgtttct
     8881 acatgtttcg gaacaatatt atttaatata acattttctc cacatgctgc agcgatacac
     8941 atatatgtgc ctgcttcaat tctatcaggt ataacttgat actcagaacc atgtaattct
     9001 ttgacgccat tgattttaat tgttgatgta cccgctccct taatattagc tcccatactt
     9061 gttaagaagt tagcaacatc aactacttcc ggttctttag cagcattttc aattacagtt
     9121 tgtcctgttg cataaactgc agctagcata atgttaattg ttgcacctac gctaaccata
     9181 tctagaaaaa tatgtgcacc ttttaactct ttagcttcga ttttcattga tgtcgtactt
     9241 gattcatcaa tttcagcacc taaagcttta aatcctttaa tatgttgatc aattggacgt
     9301 ggcccaagtg gacatcctcc cggtaaacca atcacacatt ttttaaatct acctaacatg
     9361 gcacccatca tatagtatga agcacgtaat gattcaactt tattatttgg taatgcagca
     9421 ttttgtattt cagttgtatc aacttctaat tccataccat ttagtgatgc cttaatattt
     9481 aaatcttcta ataaactcac taaagtttta acatcagaga tttgcggtaa cccttctaat
     9541 ttcacatgtc cttgagctaa taatgttgca ggaatgatag ctacagcact gtttttcgcg
     9601 ccactgatat taacttcccc atttagtgtg cgtccacctc ttatttttat tacctcttga
     9661 gccatatcat ttatctcctt tgtccattac tttttaattt tttgcattat tagcattata
     9721 aattaatttt actttatttg taaaatagtt tattttaatc gttaaaatct tgtctttcta
     9781 ttttcgattg attaataaca aatcattcac ctttactaat atatgctgtt cttccccact
     9841 tgaccatggt ctaaagtctg attattcaac taaatattga cgttgttttg acaattattt
     9901 agttttaatt ctacggtttt cacgtaaact tttttcataa attgctcatt ttccttataa
     9961 ttatatcaca atcaattttg atatatttta ccatttttac gctttcattt taaaatcaaa
    10021 ctgttttatt tattacttat acaacataaa aaagaagcaa acaccatttt gtctgcttct
    10081 tttaaaatta atatccctac gttattaata acttaaagac taaatattaa ttatttagcg
    10141 cggtttgaag taccgaactc tttaatttta cctttaactg tttctttgat agcttcacgt
    10201 gcaggtccta agtatttacg aggatcgtaa acttctttgt cgttatttaa aacgtcacga
    10261 actgcttttg ctgaagcgat ttggttttca gtgtttacgt taattttagc tgtaccaaat
    10321 ggaattgctt tttggatatc tttagttggg ataccagtac caccgtgtaa tactaatggt
    10381 aaaccagttg ataaaccgat ttcttccatt tctttgaatc ctaattttgg ttcacctttg
    10441 tatggaccat gaactgaacc taatgctggc gctaatgcat cgataccagt tttttcaact
    10501 agttcttgac attctttagg atcagcataa atgatgccgt ctgctacaac atcatcttct
    10561 tgtccaccaa cagtacctaa ttcagcttct acagaaacac ctttttcatg agcgtattca
    10621 acaacttttt tagttgttgc tacgttttct tcgaatgggc tgtgtgaagc atcgatcatt
    10681 actgatgtga aaccagcatc gatagcttct ttacattttt caaagcttga accatggtct
    10741 aaatggattg ctacaggaat agtgatgttt aagtcatgca ttaacccttc aaccatttta
    10801 acaattgtgt agaaaccgct catgtaacga gcagcacctt cagaaacacc taaaattaca
    10861 ggtgcgtttt cttcttgtga cgcttctaaa attgcttgag tgaattctag gttattaata
    10921 ttgtattgac ctaccgcata accattttct tttgcatcaa ttaacatttc tttcattgaa
    10981 actaaaggca tgaaagttcc tccttgagtg cttacgcacg tatttttcaa agtaattata
    11041 tcaaaaaaat agcgtcaatg cattgaaata ctgcagatta aatgtgtatt ttttgaaaaa
    11101 tgcgatagtt atactaaatg caaacgtata catttttata caatttaagc cgttgcataa
    11161 tatgtagtca ttaattacaa tattttataa gtagattgga ggataaagat gtcaaaaatc
    11221 ttaaacacac aactaatagg tatctttaat aggctagaaa aacaatcttt agaaattcaa
    11281 atggccgccc aatgtttaat tcaagcaatt ggtggcgaag gttatgttta tgtaaaaggt
    11341 tatgatgatt tacagttttt cgaaagtttc attctacata gtgatgagcg tttaaaatct
    11401 agccgaaaac ttgacgctat taaagacttt aaagaaattg actcaactga tcgtgtatta
    11461 ttattcgcac cattttataa tgatcaagtt gcattagata ttcaaaaact tatcgactta
    11521 gatatcgatg tggtcttaat aagcaacaaa cctaaaacag atgatttccc tgatcatctc
    11581 gtacatttta tcgatttatc tacaccaaga cctatagtct acactgaaga ttacgataag
    11641 attgtccaac cacatgcgat ggcattgaat tatgtatact atgacattta tacacaaatg
    11701 attgaaatga caagagactt agaattataa aagtcactat tgttatttca taccttgtca
    11761 ctaacataat attaaaatta tatgaaaaac cggtactttc aatgtttaac aatgaaagta
    11821 ccggttttat tagcaaattt atttattttg ttgatatttt aacgaagctt caataaatga
    11881 tttaaaaatc gggtgcggac gatttggtct agataagaat tctgggtgga attgacaagc
    11941 aataaagaaa tcatttgtcg gaatctctac catttctact aaacgtccat ctggacttgt
    12001 accagaaatt accataccat ttgcttctaa ttgttctcta tagtcattat taaattcata
    12061 acgatgacga tgtctttctt caatttccgc tttaccataa acatcttgtg ccaatgtgcc
    12121 ttctttaatt gaacatggat ataagcctaa gcgtaatgta ccacctaaat cttcgatatc
    12181 tttttgttct ggtaataaat ctataattgg gtatggtgtt gctgggtcta attcagctga
    12241 atgtgcgcct tcaaggccta atacgtttct tgaaaattca actgttgcaa gttgcattcc
    12301 taaacaaata ccaaagaatg gtacattgtt ttctctagca tacttaattg cactaatttt
    12361 accttcactt gcacggaaac caaatccacc tggtactaaa ataccgtcga catctgcaag
    12421 gtattcggct gcattttcat ctgttacttc acttgaatca atccatctaa tgtcaatatc
    12481 tttggcaaaa ggatatccag catgtttcaa tgattcaaca actgataaat atgcatcttg
    12541 taagctaaca tatttaccta ctaaaccaat tgtaatttta ccatctaaat tattaacgat
    12601 atctaacaac tgtttccatt catcaagctg tgtttcatat ttcgcgttta attgtaaacg
    12661 tttaataacg atatcatcca tattttgttg gtttaattgt aatggaattt cgtataaaga
    12721 gtctgcatca cgacattcaa taacactttc tttattaatg tcacagaata atgcaatttt
    12781 atcttttaaa tcttgtgtca tttcatattc agttcttaca acgattaagt ctggttgaat
    12841 acctaagcct cgtaattctt taacactatg ttgtgttggc ttcgttttca tttctccagc
    12901 agctttaata taaggcagta atgtacagtg aacatacata acattttctc tacctaaatc
    12961 gctacgaatt tgacgaatcg cttcaataaa cggtaatgac tcaatatcac ctgttgtacc
    13021 gccaatttca gtgataacaa cgtctgcatt cgtactttcc cctgcaagta ataaacgttc
    13081 tttaatttca tttgtaatat gcggaataac ttgaactgtt ccacctaagt aatcaccacg
    13141 acgttctttt ttcaatacgt gtgaatacac tttaccggct gtcacatttg aaaacttgtt
    13201 taaattaata tcgataaatc tttcgtaatg tcctaagtct aggtcagttt ctgcaccatc
    13261 atccgttacg aatacttcac catgttgata aggactcatt gtacctgggt caacatttaa
    13321 gtatggatcg aatttttgaa ttgttacatt tagacctcta tcttttaata atctacctag
    13381 agaagatgct gtaataccct tccctaatga tgaaactacg ccacctgtta caaaaataaa
    13441 ttttgtcatg tcttgacctc ctaattatta aagggaatgc ttcctcatca attgtaaaaa
    13501 ttacgtaaaa cataacatta aagcaagctc attcaagttg tttatttgtc gaattcgaca
    13561 aaattaaatt ctgtttactt accgtttcca tactgctatg tcactatgta tatcatttac
    13621 aactttgatt tttgcgttat catttcttga tttactcaat taaataaaaa aacgctccct
    13681 ctcacaaatt ctgtaagggg agcgtataaa ttttatacac gtgtcctatt taaaggagcc
    13741 cgaataaaat tttatcatct ttcaactaaa agtcaaacaa aaaattaatc gttgaagtct
    13801 tcttcgtctt caaacacttc ttcgtcttcg tctagttcat cttcatcttc ttcaatgact
    13861 atatccgaat gattgatttc ttcttcaacc tgctcatctt ctggatcatt tagttcttct
    13921 tgatcatctg tttgagctgg aatatcatcg tcgtcatcca tttcatcttc gcccaataat
    13981 tttaagtttt gatcttcttc atcatctgca tccagaatat cgaatttttg aatagttggt
    14041 gcgattttct cttcaatatc atctaccgaa taccaatcac gtaatcccca taaattttct
    14101 ccaacattta aaaaacgacc atctgtgttt aaatccgtgt aaaattgtac aacacgattt
    14161 tcaatttctt cgtactcata atcacctaac gctctaaatt catcgatgat atcatataag
    14221 ttcattgttt cgcctttatc attcaataat gtataagcca tatcaataaa tgatttttca
    14281 tcaaccattt gttttgtata atcttgaatt ttcattgccc aatacttcct ttcgaaggat
    14341 ataatctaaa tttatacgtc tgaattcatt tatatatagt aacaaaacta aattataaat
    14401 gacaatatca tatttcgcaa tcactgttag taatttaaat atttttcaaa aacgacataa
    14461 tcatcagttt gttcgatttc aacgaaatta ctatttagac ataatggttt aaaatcatta
    14521 ttctctccaa acagaatcat cgtaatcaca ttaaggttgt catagttttt tcttaagtaa
    14581 gttggcaatt gtcgcagcgc acttgaacca ataccattaa agcgatatga tttatccact
    14641 tcaatagcag caatgttcat gcgttcacca atctgctcca atgcgataaa gccaaccttt
    14701 tggccatcaa tcattaaatt aatgatatgt acattggtca cttctgaacg actttcttct
    14761 tcaagcttta atgacgaaac atgttttgaa tctaagtcca agtaatatag tcgttctttg
    14821 ccaattggtc cttcttcttt agtcgctgta tgcatgaatc cagcacgttc gtaaaggttc
    14881 caagcattgt cattttccgc atctactact agatatagat gattaaaatc aggaaataca
    14941 ccttgaacat attgcggcaa tgacatcatt attttcgtgc catatccaaa accttgatat
    15001 ttttcattaa tcgataatga acgaatataa acgacatttt caggtgtatc ataaccttca
    15061 tgctgatagt aacgatgtaa tacaaaaaag cccaccactt catttttatc gtttattgct
    15121 acgttagcaa tcctatcagc atcattaatt gcatcatcaa taacttcttt aggtaaagat
    15181 gaatatattt gttgtctttc attcaagtca aaatcattaa ttgctgaacg atattgttca
    15241 tcaaatgctt ttaaagtgat accttcaaaa caaagttctt tagccatata tttaacccct
    15301 atctttttat atatgaagca tgaatcccca tattatttta cctattattt cactacgata
    15361 ccattatttg ctctaagtta aataaattac ttatttatat tttttgaatt ttaaaattaa
    15421 tgtgctactc ctcattactt atcattttaa tcagagactt acaaatacac tgcgtacgaa
    15481 ctacttttaa tattgcgtca ctaactaaca atcctataca acttcaaaat tcctagaaaa
    15541 taatatatat aacttaacgt ctacgtttgt aatacattaa ttgcgatacg cttacaatgt
    15601 tgaaatacaa tggcatcaaa tttgttaata attactatta ttacttgttt tttagtcatt
    15661 ttactatact attatttaag gatagacatt aataatgagg tgggaatctt atgaaagttg
    15721 gcattgacgc tggcggtaca ctaattaaaa tcgttcaaga gcaagataac caacgtactt
    15781 ttaaaactga attaactaaa aatattgatc aagttgtgga atggttaaac caacagcaaa
    15841 ttgaaaaatt atgcttaact ggaggtaatg caggtgtcat tgctgaaaac atcaacattc
    15901 ctgcacaaat ttttgtagag tttgatgctg catctcaagg actaggtatt ttgttaaaag
    15961 agcaaggcca tgacttagca gattatattt ttgcaaatgt tggtaccggt acgtcactac
    16021 attatttcga cggccaatca caacgtcgtg taggcggtat cggtacaggt ggaggcatga
    16081 ttcaaggact cggttattta ctaagtcaaa tcactgatta taaacagtta actgacatgg
    16141 cacaacacgg tgatcgtaac actatcgatt taaaggttag acatatttat aaagataccg
    16201 aaccacccat tcccggtgat ttaacagcag ctaattttgg tcatgtacta catcatttag
    16261 atgctgattt tacacctagc aataaattag cagccgttat tggtgtcgtt ggcgaagttg
    16321 ttacgacaat ggccattact gttgcacgtg aatttaaaac tgaaaatatc gtttatatcg
    16381 gttcttcatt tcataataac gctttattac gcaaagttgt ggaagattat actgtgttac
    16441 gcggttgcaa accgtactat gttgaaaacg gcgcattttc aggtgctatt ggtgcattat
    16501 atttagaaaa atagaaaaag accatttata tgcgcgttgt tttaaataaa cgctatataa
    16561 atggcctttt attttatggt ctaagtgttt tgaatacgta ttgttccatt tattatcact
    16621 taattactta caactcaatt caattaaaat gtaagttcat tagacaattt ttgcgctgaa
    16681 acaattgtac caaccacgcc agtttttaaa tctatcgcaa cggctttatg atcatctcta
    16741 agttcatcta gccattctcc gaaaacagaa ccttcaatcg taattaaatc acactcttca
    16801 aaatcttcat ctcgaacttt taacgctctg tttttaatac tccatacagg cataatatgc
    16861 ggcttttttt cgtcgctatc attttcacta atgagaataa actgcttact acctttttta
    16921 gttaagccgt atacttcatc atatttcgct acatcttgga caaattcttt gattctaatt
    16981 ttatctaatt ccgtcatcac ttcttgtgta acagctacaa cgtttagtag atgcccttct
    17041 tcttccattg tttgattcat aataatatgg tcttgttcat caaacaattc atctaattca
    17101 taagtagcaa aacgatcaac gtccattttt acaactttat caaatggaat atcacgactt
    17161 gttaaaaatg atacggccac actttcttct gtccaaacgc ctacatacgc ttcactttta
    17221 taatttaggc gtactagttt tttattttta gaggcgaaat aaatacattc atttattaat
    17281 atatctctaa agaatggata tgctttataa gacatgcttg accctcactt tttaaaatat
    17341 gtcatacata aaatagtaaa tgactccaac aagttagtca attagacata ttatgaattc
    17401 aatttaatgt catgtttttc attttaaaaa ttcaatagca ttaaatttta cgtatacaca
    17461 aattcggtct ataacacgaa acatatctag ttactaaaaa tttcaaaacg ataaccttta
    17521 gtataccttt atcaacaaaa tggcaactct tataacatag tgaaataaaa gtcgccattt
    17581 aatattagta ttacaaagta ttgccatatc ataaatttaa ttactgttga ctagaatcga
    17641 aaaaagcttg ttacaaacgc atattcagtc aactactggc aacacaacat tgtagcgcct
    17701 tatgttccga actctaatag tgttcaagtt cagctaaaaa tgcgcgttca ttctccagta
    17761 ctgtttcttt tgaatcacca tgccaaatac agccaaacct tgatttaact ttatcttcac
    17821 cgtaccaatc accatcataa taagataacg ggtataaact accttctttg acatatttca
    17881 aaatcgtatt gaagaaatgt gtgttatcac cttttgaatg ataactataa aacttttgat
    17941 atcctgaatt caactcgtta gcaagtaata acatgctcgt tgaaccattt tgtctaaagt
    18001 ttaaatcaat cgcataaacg ttatcatcct catcgacgag taggtcaaaa ccagctacac
    18061 caaagaatcc ttgattcaca ccgttttcca taatttgtct acctgcttct ataacgtgct
    18121 ctggtacatt agtagtattt tcatttccgt tataaaatcc gtacttatcc gttaattgcg
    18181 ttgccgcacc aagatattga atgcctagcg attccgagta tgcaaactga acgcaataat
    18241 tcgctttttc ttcaatcttc tgttcgataa taagcgaatt cgtttcagca gttgcctctt
    18301 taatacgcgt tatcgctttt tgtaaatctg catcatgata acaaatcata acgccatagc
    18361 cacctgctgt cggaagatca tctcccggct taattactaa cgggaattcc caattcttaa
    18421 tctcgttttc gaattgctca atttttacaa cttttctttt tggtaaaaac ttcccatttg
    18481 tccattcagg tattcttgct ttattattta aagcaacaaa taacgtttta tctaatgcat
    18541 aatattgctg attcaagatt gtttcatcat gaatatattg aaaataaatc tttttatttt
    18601 ccttatgtgc caattgtttg atcaagtttt cgtaagattg ctgattgtta aatgtataaa
    18661 ttgagttcgg tacttcctta ccaataactt gaaatagctg atgcaatttg tctgtcgcac
    18721 tagcttcgtg aacaataaca ggtaattgat ttgctattaa taactcccta ccagttaaaa
    18781 aattagattg atgttcgtcc ggtttcaacc atggattcga tatatacgaa ggtcttgacg
    18841 tatagacaac atctttgtca tataaatcac ttaacgttaa gttcggctca ttaccattat
    18901 ttgtcattac ttcccattcc ctttcaaatg cgcatgctct tcaataatgt cttgataaac
    18961 gtcttgattt gtaattaact ctaaccccat caacgccatt attttagcgc ctttaattaa
    19021 tgcttcatca ccatgtacac tcgcagccgc ttctctaaat ctatgcgtat gtcctactaa
    19081 attacgtgat cctattttaa tatgaggatg tattgttggc acaacatgac ttacgttccc
    19141 tgtatccgta gagccataac caaaatcatc atcaataact gcttcaccaa cttcttcagc
    19201 atatttagca aataaatcat ctaatttcgg cgttttaatg aattcattca caccgttttg
    19261 aattcgacca aattcataat cacaaccagt ctgtatcgca gctccacgtg cgatttgatt
    19321 tactttttct gttaatatat ccaattcttt acgcgtcatt gctctagtat aaaaacgagc
    19381 atgtgtatag tctggaataa tattagctgc tttcccgcca tctaaaatca caccatgcac
    19441 acgttgatct tttttaatat gttgtcgtag ttgtgctaca ccattaaaat aactaatcat
    19501 agcgtctaat gcatttaacg cttcatctgc attttcagag gcatgagcac tttttccgta
    19561 aaatttaaca tctaaaacat ccactgccaa agtatcaatc gttttataag tttcatttcc
    19621 cggatgaatc attaaggcaa tgtctatttg atcaatcaca ccagccttga cataagaagc
    19681 tttagcgcta ccattttccc caccttcttc agctggacat ccaagaacga ctactttacc
    19741 accaatttgg tcaatcactt gcttcaaacc aattgcacca agcacacttg cagttccaat
    19801 gatattatga ccacaagcat gacccaaccc tggcaaagca tcgtattctg ctaaaaaacc
    19861 tatagctggc ccgtcaagac ctgaatcata tgtcgctata aacccagttg catgcccagc
    19921 aatctcggtt tcaatttcaa aatcatgctc tttcaaacga tctattaaag ttcgagacgc
    19981 aaatatttct tcattaccaa gttcaggacg ttcatgaatt ctatgactga tttcgatata
    20041 actatattta tttgtttcta tataatcgag aatttgttgt ttttcactca ttttttacta
    20101 tctccctttt accctacact cattttaatc atccacaata ttttgttctt tcaaaatgaa
    20161 ttattactta ttctatcggt tttatctcat gatgtcatct agtttttctt tatttacaaa
    20221 attttctaat aactaaaagc ctttcataaa tttataaaac agtttcaaat tgtaaaacat
    20281 aagccctatt gttacaattt aagtattgtt agaaacacaa cttacaacaa acttaagttt
    20341 tataatgcaa caaatcataa gcgttttata gttttgagta gttaatagga ggaaattcaa
    20401 aatgacaaaa atgaatgttg aaagtttcaa tttagatcat actaaagtgg ttgccccatt
    20461 tattagatta gcgggaacga tggaaggatt aaacggagat gtcattcaca aatacgacat
    20521 tcgtttcaaa caaccaaaca aagaacatat ggatatgccc ggactacatt cattagaaca
    20581 tttaatggct gaaaatatta gaaatcatag tgacaaagtt gttgatttaa gtcctatggg
    20641 ttgccaaact ggtttctatg tatcatttat taatcatgat aattatgatg atgtattaaa
    20701 tattgttgaa gcaactttaa atgatgtgct aaatgctact gaagtgcctg cttgtaatga
    20761 agtacaatgt ggctgggcag caagtcattc attagaaggt gctaaaacta tcgctcaagc
    20821 atttctagac aaacgaaacg aatggcatga tgttttcggt acaggaaaat aaatcttagt
    20881 caatcaagtt aatcagaaaa gcagtcgaac aatgatgtta caatcgccat tgtccaactg
    20941 ctttttatta tgcttcaaag tcaaaaaatc gaacaaatga aaaagtaaaa tctttaacat
    21001 ttgtccgatt tatttgagaa ccactataat ttcttaatta gtcccattaa cacgaactgc
    21061 ataggtaacc ttaaatatag ttgccatgtt ggcaattgtt tatctcctaa aggtaacttt
    21121 ttaactgcca tatagatatt agctgggaat acagctagca agaatagatt gattgtattt
    21181 ttcaagcatt gagatggtct tttaattaaa agtgcaagtc caaataatat ctcaaagatt
    21241 cctgtaacaa gaaccgctgt ttttcgaagt gggaaacatt tcggtatgat atttctaaat
    21301 tgtcgttctc gtgtaaaatg caatacacct attacactaa aacctattcc taataaatat
    21361 cttagtatgt tcaatcagct tcaactccta ttctataatg attttatgaa ttaatgtagg
    21421 cgatacaaca tgatcagcaa ttgttatgct tgaatctaat tttttaacaa catcgtctac
    21481 atcttggcga ttactgtgaa tcgttaataa tgattctcct tcttctactt tatcaccaat
    21541 ttttttattt aaaacaatac caaccgctaa atcaatatca tcctcttttg ttaaacgtcc
    21601 cgctcctaac atcatcgaag cgacacctat atcgttagag actaattcag tcacataacc
    21661 tgatttttta gctttatatt caatttgata ttgagcttgt ggcaaacgct ctggatggtc
    21721 aataacagtt tcgtcgccac cttggttttt aataaatgtt ttgaattttt ctaatgctgc
    21781 acctgaatta attgcctcaa ttagcaacgc tctcgcttct tcaagcgttt cagctttgtt
    21841 tgcaagtaca accatttgag aacctaatgt taatacaagt tctgttaaat ctttcggacc
    21901 ttgtcctttc aacgtatcaa ttgcttcttg taactcaagc gcattgccaa tcgcacgtcc
    21961 aagtggctga ttcatatcag aaataatcgc catcgtatta cgtcccacat tattaccaat
    22021 acgtaccatt gcgtgcgcta atgcttcagc atcttctaat gttttcataa atgcaccgct
    22081 accagttttt acatctaata caattgcatc tgcaccagca gcaatctttt tactcataat
    22141 tgaagaggca attaatggta ttgaattgac agtaccagta acatccctta aggcatataa
    22201 ttttttgtct gcaggagtta aatttcctga ttgtcctaca actgccactt tattttcatt
    22261 aaccaatttc acaaatgttg cttcatctat ttcaacatga aaaccatcaa ttgcttctaa
    22321 tttatcaatc gtaccacctg tatgacctaa tccacgccca ctcatttttg caacaggaac
    22381 atctacagct gctactaatg gtgctaaaac caatgtagtt gtatctccta caccacctgt
    22441 tgagtgctta tctactttga cacctttaat atcactcaaa tctatcatat caccagaatt
    22501 aaccatagcc atcgttaatg ctgcacgctc atcatcattc atatcttgga aataaatcgc
    22561 cattgctaaa cttgatgctt ggtaatcagg aatatcccct ttaacatagc cgtcaataaa
    22621 gaaattaatt tcttccgttg ttagtgtatg accgtcacgc tttttctcaa taatgtctat
    22681 cattctcatt tttatcatcc ttttcttaaa aagcttagga caaagcatct gcgctttctc
    22741 tagtccattt ttaaaagcac aagcgaaaat tattatagca agctatcgat ttcattttta
    22801 atatcacaat ttcaatgcga tgttattatt cttaaataga ttggttataa cgttaaagtc
    22861 cctattaaat taacttagaa tcatcatggc atttatgatg tcttaaagct gatatcgaca
    22921 tacttatata tggttacgat gtcccatgct tacatatttt tttataaaat tagtaatctg
    22981 aatctgcttc taaaccttgc ataatttgaa cgcctgcgct cgcaccaata cgtgtcgcac
    23041 ctgcttcaac cattttattg aaatcttcta aattacgtac gccacctgat gcttttactt
    23101 ctacatcagc acctactgta tctttcatta atttaacgtc ttctgcagtc gcaccgccac
    23161 ctgcaaaacc tgttgaagtt ttaacgaagt ccgcaccagc cgcttttgtt aattcactcg
    23221 cttttacgat ttcgtcatgg tccaacaata ccgtctcaat aatcactttt actgtgtgac
    23281 ctttcgcagc tttaaccact gcttcaatgt cttgttgtac atcatcaaaa cgtccatctt
    23341 ttaatgcgcc gatgttgatg accatgtcaa tttcatctgc accattttga atcgcatctt
    23401 ctgtttcaaa tgctttcgtc gcagttgtcg atgcacctaa tgggaatcct attaccgtac
    23461 aaactaacac ctctgaatca gctagtcgtt ctgctgcata tttaacatgc gttggattca
    23521 cacatacaga tttaaaatgg tatgctttcg cttcatcgat gatttgatcg atttgcgtac
    23581 gtgttgactc aggcttcaat aaagtgtgat caatcaattt tgcactatgc attttctatc
    23641 tcctccttta tggttgatta taaaaatacg gttgtaaatt agttgattga gcgtcaggtt
    23701 catttaaata tcaggttaga tgttcgcttt ttatgtaacc gcatacatat actattacat
    23761 taattcattt cccataaaca aacaatacaa ttgaacgtga tatcttcatt atgaacgatg
    23821 acttgacaac aagctaatca ggattatatt tttataattc tttaattcta tagtacaaaa
    23881 attcgcaaaa aagggaaaca aatgttatct taaaattatt aatgaatatt aaggagaaga
    23941 taacaaatga caaaaggtac accacatatt caaccaaatg gagtaaaaat tgctaaaaca
    24001 gtattaatgc ctggcgatcc gctacgcgca aaatatattg ctgataattt tttagaaaat
    24061 gttgaacaat ttaacgatgt acgtaacatg tttggttaca ctggtacata taaaggtaaa
    24121 gaagtttctg taatgggttc tggtatgggt attccaagta ttggtattta ctcatatgag
    24181 ttatacaact tctttgatgt agatacaatc attcgtatcg gttcttgtgg cgcattacaa
    24241 gaaaatgtta acttatacga tgttattatt gcacaagctg catcaactaa ttcaaattat
    24301 gtagatcaat acaatattcc aggtcatttc gcgcctatcg ctgacttcga gttagtaact
    24361 aaagctaaaa atgtcgctga ccaaatcggt gctactacac acgtaggtaa cgtattatct
    24421 tctgatacat tttacaatgc cgatccaaca ttcaatgatg cttggaaaaa aatgggtatt
    24481 ttaggtatcg aaatggaatc agctggttta tatttaaatg cgattcatgc tggtaaaaaa
    24541 gcacttggta ttttcacagt aagtgatcat attttacgtg acgaagctac tacacctgaa
    24601 gaacgtcaaa attcatttac acaaatgatg gaaatcgctt tagaaatcgc agagtaactt
    24661 atttaaattg actttaattg ctctttaaca atgcgattaa actcaaaaag ccaacacatt
    24721 ctgggcgtat ccccatttat gtgttggctt ttatttatat tattacttat ctgtagatta
    24781 gcttaagtaa gatttaaaca tccaattatg tttatctact gatgtttgca tacctataaa
    24841 catatcttct gatacatcat cgccagcatt accagcaatt tcgattgcgt tttctaattg
    24901 ttttgagata tttgtgaagt cttgtgataa ttcttcaacc atttgttctg cagagtaacc
    24961 tttcgcagct tctttaacaa ttgattgctc taagcattca gttaatgtac ctacagggtt
    25021 tcctcctacc gctaaaattc tttcagctaa ttcgtctaca tattggcttg cttcattata
    25081 taattcttca aatttaacgt gtaatgagaa gaagttaggt cctttcacat accagtggaa
    25141 attatgtagc tttgtgtaag ctactgtcca gtttgctact tgttgattca attcttttac
    25201 aacatcttgt tgattactca tatttaatac actccttaaa attgtctacg tcttgcttgt
    25261 ttatattaat tattatatta taatgattct aatctgtaaa gtgtttttac tctctttttt
    25321 ataattattc taatctaatc gtaaagtgtt ccgctattta aatgcctaaa ttttaattca
    25381 tcatctaata tatccattat caattagcaa gtatgtttta tcaatcagta agttttactt
    25441 tcgcattatc atctaaaaag aacgattttt cataatcatt tggttggtgc catgttgttt
    25501 tccacattct atttctatta ttagcaaaca ttcgataacc gaaattacgg actggtttag
    25561 gtacaagcca taaacctacg cccaataatt tagttcgatt aggtagtgct gtgattagtt
    25621 taataatcgc ctgcgattca aaatatatac aatcaccttt ctgtaaaatc acactatttt
    25681 tatttgccgc ttctggatgt tgtttaaaaa attgttggcc aacttcacct tttaacgtgg
    25741 caaattgata atttcttggt aggtcatgtt gaattaacca aatgacatag ttataacaat
    25801 atatacagtt tccatcataa tatacgattg gcataattta ttcgctcctt taatagtact
    25861 tatagtcatt tttcctaatt taaaaacatt taaacaaggt tgtcacttga taagtcctcg
    25921 cgacacgaaa ttgccataaa atttattttt cagttttata tttattgtat aatgtatttt
    25981 ttatttactt taaatttctc aatctacagt gtaatcttat ttcttattat taacttagcg
    26041 tttggtcatt cgtcttattc aacataccac tggaaaactc atataaagtt ttaattctgt
    26101 aacataaatt cttttatatt catatactgt ctttattatt tattaaaata aaaacgagta
    26161 atctatcata agctcttaat tagctcttat ggttgattac ccgtcatttt ttctttatgc
    26221 ggttattcaa atatgcaatt atgcaatttt taatcctatc cttacttctt ttcaaaaact
    26281 accatatatc attagtcatg tttgactttg actatacgtg tgtattttaa aaactgctct
    26341 gaatatttct ccttaatttc ctctaaactg tcatatgtaa tacctacaat atgccaatta
    26401 ggattgaaat aatagaaaga atctttttgt cgtgaccatt taatcatatt accgtcttta
    26461 ttataacgtg gtaatgttac ttcatagcct tctaacacat taatatacgt ttggaaaatg
    26521 tctgtatcta tacgattaaa attatcaata actaaataac ttttatcctt tttaggcatt
    26581 aattgagtaa taaacccttc acgatagtat aatgcccctg tttcatttgg taaatacttg
    26641 ccataaagca tatcttctga aaaatcagga tgacctgttg ttataacagg atttgcattc
    26701 gctttttgca ataagtcatt agccgctttt aagccttctt cttttcgatc tacaacaagt
    26761 aaaataaatg gctttaacgc ttcttctttt tcactttcaa gcatatctgg tttttgaacc
    26821 atttcaaatg gagatttcaa tccattatta tcgctcattt caataattgc atcatactgt
    26881 gcttgtgaca tacttgcaat agcctgtttt gcattttctt gaaggaaata taagtttttc
    26941 aatttaggat gcttatttaa tgtacttaat gtaatcggtg taatgtcttt ctcataagac
    27001 acttcaatca ctgtactatt tgttctacca ggaattggtg gtttttcatg aatatgcttt
    27061 gatacttctc caattccaac gacagattga tttttcgttc gattataaaa aataatattg
    27121 tcgccttctt ctaactgagt ataaaaatga taaccattac gtttaatacc gttgtacgtg
    27181 tgcgtataaa tcgtatattg gtttccaggt tcaaattctt cagtttcagc taaaaagaaa
    27241 taacgcggta tcttaatttc gcctttacca agaccactta ttaaatcaaa ctcttctgca
    27301 gtgatttgat tgaacaatgt ctctttcata ttactcatac gaaattccaa agcttcacta
    27361 cgctttaaat aatctgctgt taatggtttc aattgttcat taaaacgaaa ctgtacacgt
    27421 attttatttt gtgcacctgt ttcaacacta ataatttcac cacatccaag tagtccagta
    27481 tccgtctgaa cttgataaaa gatgacttga tctcctactt tagccttttt aaacgctcta
    27541 aatccttgag atgggttaaa atgtgcgcct gattcaaata aagctgtttg tcctactaac
    27601 ggttcattat gattccaacg gttatatcca caattcaacc aaaaataatt cgtttctgct
    27661 gtcatcttaa tactccttaa cctgaataaa ttttagaaac actatgaatt acattctttt
    27721 agtgtttctt atgcagttgg acgcgtatgc gaacaactgt ataccctttg ttcactgcga
    27781 ttttaatcgc atttcctata acattgtagc gcccaggaca ttaatttacg tcccagaccc
    27841 ttatcgtttt cacttctaag taagtcgaac tattttgctt tacaacaagt gcgactctaa
    27901 atacagttgg acacacatac gagcaactgt atacctttta atcagttttc tatattttat
    27961 ttattatatc tgtcttaatg ataaaaattg ttacaaacag tttaacatat ttagctacct
    28021 attttgcata taaaaaacat gcttgcatac actatgcaat aaaaattaca aatttatata
    28081 tgataccact aagcttgctt atctctatag cgccattgat acacattttt aaatatctat
    28141 actgccgtta gaattttatc atgtcttaat tttcattaaa tattaattac ttcattttat
    28201 ataaaccaac aaaaaccccc tcactactat gcaagtgaga ggttatgttg atgtgcttta
    28261 ttttcatcaa tgtcattaac acgatcatat ttcttctgtt aatgcactgt actttattca
    28321 aacgcgctat cgacaattaa tttgaagcta caaaggtacc gcataatggc agcacattaa
    28381 ttaacaatgc gctaaacttt aatgaggcgc tgatttgctt gtctttaaaa gtcaacatag
    28441 cactgataat tcctaaaata gctactatac gtggaattgt catgctcgga tactgcggta
    28501 tatgtaagaa agcaccaaga cctgctatca caccacatac taacgcaata tatagaaatt
    28561 gcttcaaatc attcactcct aaattgttat tacactatta cacatagcta atcatcaatg
    28621 tgaaatcgcc ttcaaagaca ctatccaaat cttcagaagt caaaataaag tttgtaccag
    28681 tagtcagttt gaaaatttca ccatcgacaa tcatttgtcc ttcaccttcc aacactgtaa
    28741 ctaaacagaa ctctctaggc ttcatataat ttaacgtgcc agaaatttcc catttaacca
    28801 atgtaaagaa atcattcgat acaatgtgtg tacacttatg gttttcaata atttcacttt
    28861 caggcaaaat attaggtaat ggtgcattgt actgaataac gtctaaagct ttttcaatat
    28921 ttaacggtct atcattatat tgattatctt gacgattgaa atcataaagt ctatatgtaa
    28981 tgtctgacga ttgcatcgtt tcgtatgcta atattcctga agaaatagta tgtactgttc
    29041 ctgctggaat aaaatagaat tctccaggtt ttactttaat atatctaagt atcgactcta
    29101 tcgttccgtg ttgaacatga ttcgcaactt cttctctaga ctctgctaaa gtccctataa
    29161 ctatttctgc atcttcttct gcatctataa tataccaaca ttcagatttg ccatattgcc
    29221 cgttttcatg ctcataagca taagaattat cagggtgcac atgaatagaa agtgattctc
    29281 ttgcatccac tattttagtt agaagcggaa aatctttgct tgggaaatca ccaaacaatt
    29341 cacgatgttc tgaccaaata cggtctaatg tttgaccttg atatggtcca ttaataatct
    29401 cgctcgtacc atttggatgt gctgacacac accaacattc cccagttgta tcattgtcta
    29461 attgatatcc aaactcactt agacgttgac cgccccataa ttttgttttt aaaattggtt
    29521 gtaaaaataa tggcattgtt gcacctccat tgtgattaag taagcaatag aactctgatg
    29581 ttgttgttcc attatatttt gattttgttc tcatttacat cgtattataa acttccacat
    29641 ttcaaattaa ctattagtga ttgtaccata tttactaaca ttgcagtact gccaattaaa
    29701 gcgcttcact taaatttaca gtactttaac atttttaaaa attaatagca tagagattat
    29761 ctctctctta catttgtaca catttccctt taaatttact cgcccattat accaattaat
    29821 aaacaacttt aatagttgtg ccatacattg ttcaaattct ttgtaaaatg catagacaat
    29881 acgtacttat tcatacttat aattcatcat tttcaaaaaa ataacgagtt acgaaaacgt
    29941 aacccgcttc aaatcatatt tactatcctt attaatcctt ttcaatttca aattgagtta
    30001 aagcatcttt aatgtcctgc tcaccactaa taatttgaaa ctcttggtga ttaaaatgat
    30061 tggatgtgac aatttctttt aatactgtcg caacatcttc tctaggaatt tcacctttac
    30121 catcaaaata ttgtgcagct tctatctttc cagatcctgc tgcatttgta agtgcccctg
    30181 gatgtaaaat tgtataattc aaacctgaac gtcttaaata gtcatcagcg taatgtttag
    30241 ctattgtata tggctttaaa tcaccgctat catcaaaagc ctgacgtctc gaatcatatg
    30301 ttgaaaccat gacatagtgt ttaatattag cctctttact cgcaatcatt gatttaacag
    30361 caccatctaa atcgacaata attgttttat ctgcacccgt gttccctcca gaacctactg
    30421 aaaagataac tttatcgaat ggtttaaacg tctcagttaa agtctctatt gaatcatttt
    30481 caacatcaac aagaattgct ttcatacctt gtgattttaa cgcattaagt tgatctgatt
    30541 gcctaacacc agcagtaaat ggtacatttt cttttgctaa ttgttgcact agtaacgaac
    30601 ctacaccgcc attagcacct ataaccaaaa tattcgttta caacactctc ctatttatta
    30661 ttctctatgc cataccactt tatgagatat gtaaaacttg ttacaactat aaaaatcaat
    30721 tgacatacta ctgggaacgt attaaattaa tatatgaaca aatattcata tgaaaggatt
    30781 gtcatataat gtcagaacaa tattcagaaa taaatacaga tacattagaa cgcgtaactg
    30841 aaattttcaa ggcattaggc gattacaatc gaatacgtat catggaattg ttatcagtca
    30901 gtgaagcaag tgttggtcac atttcacatc aattgaattt atctcaatca aatgtctcgc
    30961 accaattaaa attacttaaa agtgtgcatc ttgtgaaagc aaaacgacaa ggccaatcaa
    31021 tgatttattc attagatgac atccacgtag caactatgtt aaagcaagcc atacatcacg
    31081 cgaatcatcc taaagaaagt gggttataat atgtctcatt cacatcatca tcatgaccat
    31141 atgcatagtc atgtaactac aaataataag aaagtattgt ttatatcgtt tttaataatc
    31201 ggtctatata tgtttatcga aatcatcggc ggtctccttg ctaacagctt ggcattacta
    31261 tctgacggta tccatatgtt tagcgacaca ttctcattag gtgttgcact tgtcgcattt
    31321 atttatgctg aaaagaatgc cacaactaca aaaacatttg gttataaacg tttcgaagta
    31381 ctcgcagcgt tatttaacgg tgtaacgctt tttgtaataa gtattttgat tgtttttgaa
    31441 gcgattaaac gtttctttgt tccttctgaa gttcaatcaa aagaaatgtt aatcattagc
    31501 attattggtt taattgtcaa tatcgttgtt gcattcttta tgtttaaagg cggcgacact
    31561 tcacacaatt taaatatgcg tggtgctttt ctacatgtta tcggagactt attaggttca
    31621 gttggcgcca ttactgcagc tattttaatt tgggcatttg gatggacaat cgccgatcct
    31681 atcgcaagta ttttagtttc cgttattatt ttaaaaagtg cttggggtat cacaaaatct
    31741 tctattaaca ttttaatgga aggcacacca agtgatgttg atatagatga agttataact
    31801 actattaaaa aggattcacg aatacaaagt gtgcatgatt gccatgtttg gacaatttca
    31861 aatgatatga atgcattgag ttgtcatgtt gttgtagacc atacattgac aatgaaagaa
    31921 tgtgaattat tattagaaaa cattgagcat gatttattac atttaaatat tcaccatatg
    31981 actattcaat tagaaacgcc taatcacaaa catgatgaat cgattatatg ttcaggaaca
    32041 catagtcatt cacataacca tcatgctcat catcacgcgc atgcacatta ataattttaa
    32101 cctactgcca ttgcatcgat taaacttttc aatggcagta ggttttttat gtctttatgg
    32161 cgacttgttt ggtctttgat gatgcaatgt ttattaacaa attttcaact attatttctt
    32221 acattagtca tatttttgac aatttactat tataattctc taactttagt cactttaatt
    32281 aatttttatt agatattaat atgaaaataa cgtgtttttt gttattgtat ttatatgaag
    32341 aaaaggaggc accactttgt ttacttactt taaatcagca tttaaaaatg ctaaaccaca
    32401 attattaata acattgatat atgcacttat agcatttgct gtaattgcag ttgtatattt
    32461 acttgctaat ttccagcttg ctaagtatgt acaaactatt gcaatttatt cacaatttgg
    32521 gcaaaaacca ccagtagatg catatttaaa agttatcgct gtattactta tagcagctgt
    32581 agtttcactg tttgttttag ttcaaatttt tatcggaatt acaaatgtta tgaaacgagc
    32641 aatgagtcat gaaaaagtaa aatttaccga tttatttatt gcatttaaaa aaggtaatta
    32701 tttaaagagc gttctgatag gtcttgtatc tatagcaatg attatcgttc tttcacttct
    32761 gacatcatta ctatacaaat tattttctcc agtatcagaa atgattatga attcagtaca
    32821 atcatcatat gcagatagta cacacctgat tggtattgct attacaactc aaagcatcat
    32881 aatcattgtt gttttattga ttaaagcaat tattacttgg ttattgttaa tacctgtttt
    32941 caactttatg actagtttcg ttgaatcaac taacgataaa gtaaaaacac acttagctaa
    33001 tggatttaaa gcaatgaaaa atggtcaaaa aacatttttc aaatt
//
LOCUS       DAMZLE010000014        32581 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMZLE010000014 DAMZLE010000000
VERSION     DAMZLE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.014"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(205..732)
                     /gene="isaB"
                     /locus_tag="POB92_000666"
     CDS             complement(205..732)
                     /gene="isaB"
                     /locus_tag="POB92_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDI6578186.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            990..1448
                     /locus_tag="POB92_000667"
     CDS             990..1448
                     /locus_tag="POB92_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578187.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKATHK
                     KTETEENDQQDDSADKADKNQNNGNNVTNDNQPSSQSNVPNNHSTAPSNTQPAQPKDS
                     NSNKNNTHDGGSQSSTNNQNNQQQNTPPANKSNNTNTSSKQTPQAQSPKN"
     gene            1688..3562
                     /locus_tag="POB92_000668"
     CDS             1688..3562
                     /locus_tag="POB92_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6578188.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHIPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRLVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            3639..5591
                     /locus_tag="POB92_000669"
     CDS             3639..5591
                     /locus_tag="POB92_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDI6578189.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDKTAKHSDLNKHSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            5605..6543
                     /gene="manA"
                     /locus_tag="POB92_000670"
     CDS             5605..6543
                     /gene="manA"
                     /locus_tag="POB92_000670"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDI6578190.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(6655..9636)
                     /locus_tag="POB92_000671"
     CDS             complement(6655..9636)
                     /locus_tag="POB92_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI6578191.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDIIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            9847..11706
                     /locus_tag="POB92_000672"
     CDS             9847..11706
                     /locus_tag="POB92_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDI6578192.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNKL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(11971..12531)
                     /locus_tag="POB92_000673"
     CDS             complement(11971..12531)
                     /locus_tag="POB92_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6578193.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(12700..14607)
                     /gene="sasF"
                     /locus_tag="POB92_000674"
     CDS             complement(12700..14607)
                     /gene="sasF"
                     /locus_tag="POB92_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDI6578194.1"
                     /translation="MAKYRGEPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMDGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKYASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(14849..16207)
                     /gene="gtfB"
                     /locus_tag="POB92_000675"
     CDS             complement(14849..16207)
                     /gene="gtfB"
                     /locus_tag="POB92_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDI6578195.1"
                     /translation="MIKLFDYFNNHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFDHNDEITMESKDQLIQMLESLKDQQQFQDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(16200..17708)
                     /gene="gtfA"
                     /locus_tag="POB92_000676"
     CDS             complement(16200..17708)
                     /gene="gtfA"
                     /locus_tag="POB92_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDI6578196.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(17726..20116)
                     /gene="secA2"
                     /locus_tag="POB92_000677"
     CDS             complement(17726..20116)
                     /gene="secA2"
                     /locus_tag="POB92_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDI6578197.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWGDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNLYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(20106..21065)
                     /gene="asp3"
                     /locus_tag="POB92_000678"
     CDS             complement(20106..21065)
                     /gene="asp3"
                     /locus_tag="POB92_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDI6578198.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLVFRCLTITEMTHQDDLEYKSMRVTKID
                     DNQYGNDKINVIIAEPSDVYPTISNDFLKLFGHVWLVERWMEDDIKENIKQLKDDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLKEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(21043..22611)
                     /gene="asp2"
                     /locus_tag="POB92_000679"
     CDS             complement(21043..22611)
                     /gene="asp2"
                     /locus_tag="POB92_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDI6578199.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPNAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(22601..24154)
                     /gene="asp1"
                     /locus_tag="POB92_000680"
     CDS             complement(22601..24154)
                     /gene="asp1"
                     /locus_tag="POB92_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDI6578200.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(24165..25376)
                     /gene="secY2"
                     /locus_tag="POB92_000681"
     CDS             complement(24165..25376)
                     /gene="secY2"
                     /locus_tag="POB92_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDI6578201.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTIIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLSQYW"
     gene            complement(25506..32333)
                     /gene="sasA"
                     /locus_tag="POB92_000682"
     CDS             complement(25506..32333)
                     /gene="sasA"
                     /locus_tag="POB92_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDI6578202.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGNGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLKVQPTNNTFQDFDINYNGDTKVMTVTYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPVVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIDTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSTSESSSTSASLSDS
                     TSNAISTSESLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSLSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQSMSGSTYNSTSVSDSESVSTS
                     TSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHT
                     HSTSTSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGG
                     VMTLLVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT        10062 a         6060 c         5130 g        11329 t
ORIGIN      
        1 aaaatattaa caaatacaaa atagtgctct atacacttaa tcaatgtttg aaatatgtga
       61 gataactatt taaataatca tgatcaaata aacattttta tcaatttcaa acaactatgt
      121 aagacgcaat aatcgttatt atttaataaa aaagactaga atgcggtatc tcccctcaca
      181 ctctagcctt aatatgccat tattttattt acttgttttg tatggtgtat gtccaactgt
      241 tacttcgaca actctattat gatcaacgac aaaccagatg cctaatgtct ttttagcatt
      301 ttgatagtaa aaaataccgc tatcagcttc ctttgtttta ccattttctt ttttcaagtc
      361 cttgccataa gctttttgga tatcttttaa tgaaatatta tttttaacta caaattgcaa
      421 ttgacttgct tcgtttcctt ttgcagaaac acctttaaaa gtttgatcat atttctctac
      481 tttttttatc gtattcgttt tagctaattt aataccattg aatttcacat tatcatactt
      541 aatcgcgtta ataaaattct tatccagaat aaaattagca ttattaccaa tataaccatt
      601 ataagtataa tatggggtta ttgctgcctg tgcctgttta cttgttggcg ccgaattttc
      661 aacaactgtt actccaatca gtgtgcccaa tgctaatgtt gctgctacac aaactttact
      721 ggttttattc ataattatct ctcttttcca tacaaatatt ttattcctta cacttttatt
      781 ataacttttt cagccataaa aatactaatg attatattta taaatacaca acagttttgt
      841 tatactaatt gaagtacaca aaaaacaatg aaaatattaa tatcgcctac ttttctaata
      901 tttatttttt aaaatgatta tggcaatatt gaaataaatt atttagcttc acgtatcaac
      961 tacttttcat taacatggag gcgttatatt tgaaaaaact tcttattacc atcgtaatta
     1021 tcgtattaat cggagcactt gggttcgcaa tttatgctgc aataaatcat tctacatcat
     1081 caagtaataa ccaagaacaa gaaaataaag ctacgcataa aaagacagaa accgaagaaa
     1141 atgatcaaca agatgatagt gcagacaaag cagataaaaa tcaaaataat ggcaataacg
     1201 ttactaatga caatcaacca tcttcacagt caaatgttcc taacaaccat tcaacagcac
     1261 catcgaatac gcaaccagct cagccaaaag actcaaattc aaataaaaat aatactcacg
     1321 atggtggtag tcaatcgtca acaaacaacc aaaataatca acaacagaac acaccacctg
     1381 ctaataaatc aaataatacc aatacgtctt ctaaacaaac acctcaagca caatcaccta
     1441 aaaattaatc atttacctga tacgtccaca agtatcaggt acataaattc agaagaacag
     1501 caatatgata tttttctatt tgaaatatct tactgctgtt ctttatttgc ataattcgca
     1561 ctctgttaaa ttcatataaa agttcgcttt aatgtcattg acgtatgtat aagctctcat
     1621 gtttaataat attaatacac ctttaaaaac gcttacacgg aaaatttaga aaggaggtac
     1681 atgttaaatg cttgatagac acttaaaact actccagttt tttattaaaa atccatcaaa
     1741 gcacattagt tctaatgaaa ttgctgaaca tgtcaatgtt tcaaaccgca cagttcgcaa
     1801 tgatattcat gttatcaata gcaattttat ggatgatatc atcgttagta ttaaatctaa
     1861 aggatatcaa ttaaatacat cgcagtatac cttagaaacg ataactgaac gttacacgca
     1921 tattcaatca tataaagaaa aactattatt atctatggcg tatcaactgc taatgcacaa
     1981 taaatcgcag acattgcaac aactagagca agattatcta ttatctaaaa ctgtgctcaa
     2041 cgactatttc gttcgaattc aacaatggtg tcaaaaattt aatatcgctt taacaatcaa
     2101 gaaaaaacaa ggtattgtgg tcgatggaac agataatgat atcactaatg ccatcatcca
     2161 tttaaatcaa ttatcatcag ggcatgtaca tgttgaagac ctaattttaa atgaattacc
     2221 tgattcacac caaaggatga tttcgcatat tatacaagag acacttcaac aacatagtat
     2281 tgaaacaact gatattcaaa tacaacagct tttaattcat ctcattttaa tcattaaacg
     2341 taagcaatct ttaagtgagc tagacacgtt aaaccacgat tcaaaattga tatctcaatc
     2401 ttgcattaag caaatcaacg aaagattagg ttatgacttg aacgaaaaag taatcaatat
     2461 gttttcattc tttattgctt atcattttaa taaactcgat attggcatag aacgtatatt
     2521 tatacaaagt tatattgatc gcttaatcga attgatgcag caacgtattc atataccttt
     2581 caatgaagat tcgattttac aacaaaattt atataatcat tttagtaaag catatttacg
     2641 tatcactcaa aacatttatt taaacaatcc tttagttata gagattaaaa aactctatcc
     2701 ctttgtgttt aacacacttt ttgaagcgat tgataaatta gcaattgata ctgatataga
     2761 gatgagcgaa gatgaaattg catttttaac aattcatttt caagctgcca tcgagcgtcg
     2821 taccaaaaca caattaaatg tagtcattgc ttgttattat ggtctaggtg tgtcgaattt
     2881 tttagaaact aaaataaata acttatccga agagttgtca gtgataaata cgattaaact
     2941 agaaaatata acacactatc actttgacaa tgtagattta ttaataacga cccatgatat
     3001 tccaaaacaa acgctaaaca tacttcctaa gcacctaacg actatcaagg ttgcaccatt
     3061 attttctgaa gatgaccgtc acaaaatcag actcgttgtg aagcaaaagc aaaatccagt
     3121 tcaagcacat catcatatgg acactgtcaa tttccttgtc gtaaatactg aacaaaaatc
     3181 acggcatact gtgcaaattt ttgaagaagc tcaaaaaata ttacaagcac atcatgcgat
     3241 tgtagaagga tatatcgaat cagctttaga gcgtgagaaa tcatcttcta catacatagg
     3301 taattttatg gcgattcctc atggagatcc tgaaaaagtt ttacaatcac atgtgcttat
     3361 ttttcgcaca aaagatgttt ttccttggcg acaacacgat gtcaaacttg tctttttctt
     3421 agcgatttcg cataaagaca aagcttttac aaaacaaatg atgcaattaa ttgctaactt
     3481 ggatgatgat tcagtgaacc atttatgtag tttagatgat catagtttaa agcaacagtt
     3541 atttgaatac ttacaagaat aattgccact agtaacggta attattcttt tttttagtat
     3601 cgagaatacg cttacaatat agaaaaggag atgattttat gaaaattgta gctattacct
     3661 catgcccaaa tggcattgca catacatata tggctcaaga aaagcttgag caggtagcaa
     3721 aagaaatggg tgtggatatt aaagtagaga cacagggcgg tgttggtgct gaaaatgttt
     3781 taactacgca agacatcgaa gaagctgacg gtgtcataat tgcagctgat aaacaagttg
     3841 atctgtcccg atttgttggt aaacggttga ttaatgaaaa tgttcgcgaa gggattcata
     3901 atccgagagg tctaattcaa cgtatcatta atcaagatgc gcctatttat caatctgaaa
     3961 caaattatca ttcgaaagat cgcggtaagt ctaaaagtgg tattcaaatg gtgtatcaac
     4021 atttaatgaa cggtgtatcg tttatggttc cttttatcgt agttggtgga ctccttatcg
     4081 ccatcgcgct gactctaggc ggtgaaacga catcaaaagg attagtcatc ccagatgatt
     4141 cattttggaa atccattgaa aacattggta gtttagcctt taaatttatg gttccaatac
     4201 tagctggata tatcgctgtt agtatcgctg acaagcccgg tcttgttcct ggtatgattg
     4261 gcggcgctat cgctgctgac ggtagttttt atggcagtga tgcaggcgca ggatttttag
     4321 gtggtattgt tgctggtttc ttagcaggtt atatcgcaaa gtggattaaa gatattaaag
     4381 ttccaaaagc aatggcacca attatgccta tcattattat tcccattatt tcatcggtcg
     4441 ttgtaggatt gatatttata tttttaatcg gtgcaccaat ttctaatatt tttgaggcat
     4501 taacaacctg gttaaaaagt atgcaaggtg ctaatattat tatacttgcg atgattatag
     4561 gtgcgatgat tgccttcgat atgggcggtc cagtaaacaa agtagcattt ttatttggct
     4621 cagcacttat tgctgaaggt aattacgccg ttatgggtat ggtcgcagtt gctgtatgta
     4681 caccaccaat cggtttaggt ttagcaacgt ttgttcaaaa gtataaattc aatcattccg
     4741 aaagagaaat gggtaaggct tccttcacta tgggactgtt tggtattact gaaggtgcta
     4801 ttcctttcgc agcccaagat ccattgcgca ttatacctgc aaacatcatt ggtgcaatga
     4861 ttgcatcagt cattgcagcc attggtggtg tcggcgatag agtcgcacat ggaggtccaa
     4921 tcgtggctgt attaggtggt attgatcatg tattatggtt tatctttgca gtgatagtag
     4981 gtagtcttgt cacaatgact actgtactat tattaaaacg caatacacca gttatagcag
     5041 tagatgcacc agcgcaacac acgcaacttc atgatacaga tattacacaa catgatacag
     5101 aggttgacaa tgtagataaa accgcgaaac attcagatct caataaacat tctcatgttt
     5161 ttgatcaaca aactatgata attactgatc acgacatgtc tcgaaacgaa gcaattgata
     5221 tgttgattca taaacttaaa atttgccgtt atgtagagca cacatcgcat ttaaaaaatg
     5281 caatcctaga acgtgaaatg gagtctacga cagctattgg catgaacgtg gccattccac
     5341 atgcaaagtc tgatgttgta aaacaaccga ttgtagctgt tatgaaaaat aatcatggtg
     5401 ttaaatggga cagtctagat ggtagtctac ctcaattgat attcttaatt gccgtaccaa
     5461 acaacagtca ggatacacat ttaaaaatac ttcagcgctt atctaaagcg ttaatgaatg
     5521 atgagacacg ccaatcacta attaatgcaa acagtactac agaaatttat aacttattaa
     5581 tgaaaatata gaggagtttt aactatggca ttatttttac aacccgtttt taaagacaga
     5641 atttggggcg gtcatgcatt aaaagcattt aactatgata tccctaatga gacaactggt
     5701 gaatgctggg caatttctgc acatccaaat ggtcctaata caattatcaa tggtccatac
     5761 aaggatatga ccctagatca actttggtca caacatcgtg aattattcga taatgattca
     5821 cgagatagtt tccctctgct aactaaagta ttagatgcca atgataaatt atctgttcaa
     5881 gttcatccag atgatgacta cgctttaaaa cacgaaggcg aactaggtaa aacagaatgt
     5941 tggtatattt tagatgcagc cccaggtgct gaaattatat atggcgtaca tgcacataat
     6001 aaacagacgt taatagacat gattgataat catgaatttg acagtctttt caaacgtata
     6061 cctgttaagc ccggtgactt ttattatgtg cctgctggca ctgtccatgc cattggttcg
     6121 ggtattttaa ttttagagac acaacaatca tccgatacga cataccgtat ttatgactat
     6181 gatagacgag atcaaaatgg tcaattgcgt gatttgcact tagagcaaag taaagatgtg
     6241 attgaattag gcaatcatga accaaatact actcctataa caactcacat cgacacacac
     6301 actgtaactc aatttgtaga taatcaatat tttgcagtat ataaatggga tattcatgga
     6361 gatttaaaat tcaataaacc acatgcttat tgcttagtta ctattatcga tggtactggt
     6421 actttaacag taaacgataa gcactatgac atccaaaaag gttctagctt tattttaact
     6481 acggaagacg aggatattca ctttgtcggt aatattagtg ctatagtaag tcatcctgga
     6541 taatagcaat gataacaccc gtcccataaa agacagaaat tactatctct tctgtctaaa
     6601 aacataaaaa tccacacatt aaggagctat aaccctaatg tgtggattta atttttattc
     6661 tgtaacgtta ctttggtcaa ctttttcaga tacgcgcttc atcaatggat ctgttacagg
     6721 ttttaaaatt aaacctacaa cgaagaatcc tatgccaaat aacgttaata taattaattt
     6781 tgtaattagg atttccggaa caataccgcc tactgtttca cgtaatgaat caattgcata
     6841 cgtaaatggt aagtatggcg aaatgttttg gaaaaattgt ggcgtagttt gaattgggaa
     6901 tgttcctcca ccacctgcaa tttgtaatac aagcaatacg attgcaatgg ctttacctgg
     6961 attaccaagt aatgatacgc acgtatatac gattgagttg aaaataatcg aacagaaaat
     7021 cgttataagt acaaataata caggtgactc aactcctgct tttaggatta acaaatctcc
     7081 aaccgataca atgagtgctt gcaacatacc aagcattata aagaatcctg ccttacctaa
     7141 gaacacttgt cttgtcgtta agactgactc tagactctta tgtttattat caaccgttaa
     7201 taaacttacc atcaagagtg cacctaccca tactgacagt gcagtataga atggtgtcat
     7261 acctgaacca taatccttaa ctgggaaaac atcaacagtc ttcttattaa taggatttgc
     7321 aataataccc gcttgctttt tcaaatcatt cttaagtgtg tcgattaatt tgtcgactgc
     7381 atcatctttg tctaatttct tgaaaatttt attcgctttt ttcaaatctt tttcaacatc
     7441 aggcaattta ttttgagaaa aatcagctaa atctgataaa gcctttttcg cttgtggctg
     7501 atttttattt agtaatccta ccgcttgatc ataaccattt aataacgttg gtaaattttt
     7561 attcacactt gctgtcgcat tagttaatcg ctgttccaac tgtggtaagt cattgcgcac
     7621 aaagttagca gctttagcta catcactttt cactttcggg aagtttgctt gagcattttt
     7681 aacagcacta atgtattgtt gttcaatact tggtaatact gtttgtaagc gatcaatacg
     7741 tgttttacca ttttttaaaa tagcttgtcc actgttaatg acttgatcaa ttgtatttaa
     7801 tttagactga acattggata atgcagtttg ccctttgttc aattcatcca taatacgttg
     7861 tgatattgat actaaagagc tgttaagatc ttttttaaca taatctctaa atgatgataa
     7921 tgatgaatct agttttgaaa gttgatctag taatttagta gcttcagcag ttcctgaact
     7981 accattcttt aatgcattgc ttaattgatt aactaacctt aatgattcat taattcgatt
     8041 attagctgct tttaccttat caatttcttt tgataaatca acattttcat tcttctcaat
     8101 ctcttttaat gtatcgataa attgttgatt atattgcgtt gtgtattcta aaccggactt
     8161 aacattatct aaagactctc taaaatcaga aggcttgtcc gatgctaaaa tgccatacga
     8221 aatattacgt aatgcctgac tatctttttg tgcggctttc gcttgggtat ctaaattatt
     8281 tgataatgat aataaagagc ccgttaatgc cgcttccatt gatttaacat ctttatctgt
     8341 aatgttttgt gtgttctcgt tagctgtgct gacttgtgtt gttgttatat ctgcaaaact
     8401 ttgaccatct tttttcccgc ttgtactttg tggtgtagtc gatacaggcg cattattact
     8461 atttggtgcg gtattttgcg taacattatt atttggtgca gtatcgccac ttggtgcact
     8521 tggtggcgtt gatgctacac cattaccagc tgcaggtgca ctatttgatg tttcgttgtt
     8581 cgccgcgcta ttttgaggaa tctgttgctg gtttcttcga tttaaatctt gcgcgcgtcg
     8641 aattgcatct tgataatcac cgactctact tctaacttgt gtgacgaagc ctttggcatc
     8701 atttaactgt ccattagctt gatcaatccc ttgactcgca atgttaagtc cttgattaat
     8761 tttagggaac tgcgctggca cgtcatcagc tgcaaaattt aacgcttttt caattttcgg
     8821 catataatta tttaaagcta atatcaactt agccttttca ttaagttgcg gaatagcacc
     8881 attgacttcg tttaattttt tctgagcatc taaaatatca cctttataat ttcctagttt
     8941 tctaaaatca ttggcatatt tatctaaatc cgcttggtgg ttattcaaat atacaatttt
     9001 attcgcaaag tcattaatct taggtaaagc tttatctgcc gaatataccg cattttttat
     9061 cttattaatt gtcggcacat tttcttcaat agttaaacct gctttgttag cttcttctaa
     9121 taatgctcga gttactgttt tattaaattg ttcattcgct ttttcaacga caactgacga
     9181 accagtatct gttagcttag acgcaacagc gttaatcttc tgattcacct taaattctac
     9241 atctgctttt tgaggctgct tacgtagtgt cccggtaatt tcatgtgtaa acttagatgg
     9301 gatgtaaata cctgcaaaat atttacccat ttttatctca tgatcagctt tctctctact
     9361 tacaaactgc caatcaaaac ttttattttt cttgagtgta ttaaccatcg tattaccgac
     9421 attaactttt ttccctctga ttatgtcgcc tttatcttca ttaacgactg cgaccttgat
     9481 gtgtcccgtg ttgccatatg gatcccacat tgcccataag ttaaaccaag cgtagaacga
     9541 tggcaaaata gctaagcctg ctaagataat ccacacagct ggcgtcttag ctactttctt
     9601 cagatccatt ttaaataatt taaatgcgtt cttcattgtc acactcctat gtaggaatta
     9661 ttcatatttt ttatatattt tttgtaaatt aatttatttt tgcgttgtga attagtataa
     9721 tcaatttact ggaagatatt tagtcgattg atacctatca actattttca gcatacgata
     9781 aattataaca aatcatagtt tattatcaca cttaattatt atatttttca agggagaata
     9841 cgaaatatgc ctaaaaataa aattttaatt tatttgctat caactacgct cgtattacct
     9901 actttagttt cacctaccgc ttatgctgat acacctcaaa aagatactac agctaagaca
     9961 acatctcatg attcaaaaaa atctaatgac gatgaaactt ctaaggatac tacaagtaaa
    10021 gatattgata aagcagacaa caataataca agtaaccaag acaataacga caaaaaattt
    10081 aaaactatag acgacagcac ttcagactct aacaatatca ttgattttat ttataagaat
    10141 ttaccacaaa ccaatataaa ccaattgcta accaaaaata aatacgatga taattactca
    10201 ttaacaactt taatccaaaa cctattcaat ttaaattcgg atatttctga ttacgaacaa
    10261 cctcgtaatg gcgaaaagtc aacaaatgat tcgaataaaa acagtgacaa tagcatcaaa
    10321 aatgacactg atacgcaatc atctaaacaa gataaagcag acaatcaaaa agcacctaaa
    10381 tcaaacaata caaaaccaag tacatctaat aagcaaccaa attcgccaaa gccaacacaa
    10441 cctaatcaat caaatagtca accagcaagt gacgataaag caaatcaaaa atcttcatcg
    10501 aaagataatc aatcaatgtc agattcggct ttagactcta ttttggatca atacagtgaa
    10561 gatgcaaaga aaacacaaaa agattatgca tctcaatcta aaaaagacaa aaatgaaaaa
    10621 tctaatacaa agaatccaca gttaccaaca caagatgaat tgaaacataa atctaaacct
    10681 gctcaatcat tcaataacga tgttaatcaa aaggatacac gtgcaacatc attattcgaa
    10741 acagatccta gtatatctaa caatgatgat agcggacaat ttaacgttgt tgactcaaaa
    10801 gatacacgtc aatttgtcaa atcaattgct aaagatgcac atcgcattgg tcaagataac
    10861 gatatttatg cgtctgtcat gattgcccaa gcaatcttag aatctgactc aggtcgtagt
    10921 gctttagcta agtcaccaaa ccataattta ttcggtatca aaggtgcttt tgaagggaat
    10981 tctgttcctt ttaacacatt agaagctgat ggtaataaat tgtatagtat taatgctgga
    11041 ttccgaaaat atccaagcac gaaagaatca ctaaaagatt actctgacct tattaaaaat
    11101 ggtattgatg gcaatcgaac aatttataaa ccaacatgga aatcggaagc cgattcttat
    11161 aaagatgcaa catcacactt atctaaaaca tatgctacag atccaaacta tgctaagaaa
    11221 ttaaacagta ttattaaaca ctatcaatta actcagtttg acgatgaacg catgccagat
    11281 ttagataaat atgaacgttc tatcaaggat tatgatgatt catcagatga attcaaacct
    11341 ttccgtgagg tatctgatag tatgccatat ccacatggtc aatgtacttg gtacgtatat
    11401 aaccgtatga aacaatttgg tacatctatc tcaggtgatt taggtgatgc acataattgg
    11461 aataatcgag ctcaataccg tgattatcaa gtaagtcata caccaaaacg tcatgctgct
    11521 gttgtatttg aggctggaca atttggtgca gatcaacatt acggtcatgt agcatttgtt
    11581 gaaaaagtta acagtgatgg ttctatcgtt atttcagaat ccaatgttaa aggattaggt
    11641 atcatttctc atagaactat caatgcagct gccgctgaag aattatcata tattacaggt
    11701 aaataagtat tattaaaccc gtaaaattta taagtataaa caaggagttc ggacttaaac
    11761 atatttctgt tcataagtcc gatttcttat tcaattaaac ccgaggcatt cagttcgaac
    11821 gcctcgggtc attttatata aatatattat tttatgttca aatgttcctc atcatatccg
    11881 tttcaattgc catctcacac attttataaa tatgagcaaa tgtacttatt ctcaaacatt
    11941 actgccgagc tttaattgac gttatattaa ctataaacta cttttccatg actctacgga
    12001 ttcaatgtca catgagcgtg ataaaatttg ttcaataata aagtcatgtt tatcatctga
    12061 tctatcacca acagcatctt ctaaaacagt aatataatag tctttatcta cactttctaa
    12121 tgccgtgctc aatacagctc cacttgtaga gacacccgtt aatactaaat gattaatatc
    12181 atttgcacgt aaataaactt ccaagtaact acctgtaaat gcgctaaagc gtcgcttaga
    12241 aataatcggc tcatcttcta gtggtgctaa atcttcaagt attcgtgtag atgcatctgc
    12301 ttcagtaatc gcatatcctt gagctttaat tgttgaaaac actttattac tcgaggagac
    12361 atcattaaaa tgcttatcta acactaaacg tatgaaaatg actggtattc gatgttgtct
    12421 tgctgcttca attgctctct gattcgcttt aataatattt tttattctag gtacactact
    12481 cgctatacct tcttgcatat ccaaaactaa tagcgccgtt tttcgagaca tcttcattct
    12541 cctttacttg tgtagttcta agtcgttaaa ttcattataa cgttaaaatg atggacaatc
    12601 tattcattgc attttgcata tacttcacaa taatttaagg ggaaataaga cgtcttatat
    12661 acttaaaaaa aatatataga tgctcttccc ccaatataat tatgctttat ttttcaactt
    12721 attgcgtcgt gataaccaaa tcattagtac acccattgca ccaacaatta cagatatcgg
    12781 caaccaatgt tcttttatcg tttcccccgc tttaggcaaa gatacattac catcagcatt
    12841 taataatcca cttaacaatc cattaccttt accaagtgtt aacgtctttt ctggctttgg
    12901 tgtgggtata tctggaatac tgtctaataa atttgatcct tgattcatta aatttgctaa
    12961 cttatttaaa tccgtcgttt tcccattttt attcaatcga tctagtaaac ttggacgatt
    13021 tactattggt gataaaatat agtctatatc ttttttcgtt tgattgagtc tcttttgtaa
    13081 attcaataaa tcatccgctt taccattcaa tgccgattta actaaattaa aaattttatt
    13141 ttgatctgtt tctattttag taattaaatc tgccagtaat tttgcctttt gtctttctat
    13201 acgtgttgct aaaatcgttt caattgcttg ttttttatct ttcgcattat tcaaaattgc
    13261 ttttaatata tcatctgaag acgtgtcgcc agttgatgca aaatgtttct tcaattggtc
    13321 aacgatttgg cgatttgata atcctttatt cgtccaatct ttagccaatt tatctgcttc
    13381 agcttttcct aatttcgttt gtaaaatttg agaaatcaat agcgacttat cttgtgattg
    13441 atcaatcaat gacgttaata aatcatcact cgttgtcaga gatagttgat caatatgacg
    13501 agtaatttga tctgcaattt gttgatccgt tttaccatca acacgtatat cttttagaat
    13561 tttatctgcc tcgtctttat taaatatact ttctaaaatg ctttgtgtag catacttttt
    13621 atcatcagta cgtgcaagtt cttccaaaat aatatttcgt tgacttttta tacgctcttt
    13681 cgtcttattt acttcgctca ttaagtctga tttttgattt ttaggaagtt gcgtatttgc
    13741 aatacgttga tctaaagatt gtaacgtatt cagtttatga tatgtgtaat gttgcgttga
    13801 ggcattactt ttagccaatt tttcaatcat agcatgatta attttatcgc ttccttgtaa
    13861 tttatcagtg agtcgattac tatggctttg attctcttca tttgaaagga atttatttaa
    13921 cacaacatgt ccagaaccat cattatttgg cgttttagct acttcatgat tactatctgt
    13981 tgtagacact gctggatctt tcgatgcata tttcaatgca tctttcgatt tgtgtatttg
    14041 ctgattcaaa tggtctaggt cttctaacgc cttatttacc attgcttcat catttttatc
    14101 atctttttct ccatgttttg ttgtagccgt ttgtgacata tcatttttca ttgcattaag
    14161 atcgtcctcg ccactttgtt gacccctatc aacatttgaa gaaacctcat ttaaatcttt
    14221 aagcaattga tctaatttac tgtctatatc actttgaccg tccatttcag tgtgagaact
    14281 tttattgtct ttgctatcca actcattagc tcgttttatg atttcatcta tttgcgatgc
    14341 tgttttcgct tcatttagtt gtgctttata atgtgcttta gatgaagccg ataactgttt
    14401 taattgctca atttgacgaa ttgctttgtc aactttgtct aataaatctt gcttagataa
    14461 tatctctttt gaaatttcag tatccttttc agatgcagct tgggcatcgt acggcaagat
    14521 attcgttaaa atgatacttg tcgccatcat tgtcgaacac gataacttta catataattg
    14581 aaacggttcc cctcgatatt tagccatcaa tatactcctt cctcacttac ttccttcaaa
    14641 gaattacata ctattatata cctgttcaca agaaatttat acttatctat ctagttattg
    14701 ttgttagtaa ttatctactt attacttagc ttatatttaa gtaaacaaaa caagcatgac
    14761 gtaatatcat attgtccatg tcgcttacat catattacgt caaatctttt atattaaatg
    14821 atgctttatt ttagactgct ttttcttttt agctttcgag cgcctgttta aaaacttgct
    14881 cgaattgttc acgcgagatt tcgtgtgcat gtgctttttg tgctaataaa gcatcttgaa
    14941 actgttgttg atctttcaaa ctctctaaca tttgtattaa ttggtcttta ctttccattg
    15001 ttatctcatc attatggtca aataagtgct ctgataatgt tactttagca tggtgtgcgg
    15061 tttgacgata acctaaaatc aacaactcat agtcaaacgc ttgttccacc gcatttaaaa
    15121 tttcattacc ctcattgata tcaagataaa tatcacataa ctggtatagt tcatttaccc
    15181 tatctatatt aatagatggg tataaatgca cattagcata ttgatcaagt tgcattagct
    15241 tatcagacat ctctgtaata gcagcgatgt gaaacttaaa atctggtaaa gtttcaacca
    15301 ataccttgat gttacgaagt tgatccgagt tagttaatat tacaatttct ttagtatatc
    15361 tattacgact acgatagtta tatagatatc cgccttgtaa aatacgagat tgaacctttg
    15421 cgtctgctat attgagcatc gtttcatatt cgtttttatc tggaataata atattacaat
    15481 gtcgtttcat atcaccttta cacatcaatt gcatatttcc cgggacatta ccattacagt
    15541 gttcttgcca taccaaaaca tcactacctt ttgatggcaa attatataac actgaaaatg
    15601 gtagggctag tgagttaata acgaaatgat gttccgtaat ttcaagttgc ttgataaaaa
    15661 ataatacgaa tgcgagcttt gaagggaaaa agtaagactt cccttgccaa tccaatatga
    15721 catcagatgt tacaaaattt tcataaatca cttctttacc ttctgctgtc atatatttct
    15781 tcaagatcgc tttacgattt aaatcgtaaa cagtttgtgc aaatttaata ccattcttag
    15841 aataataatc gacaaatcgg acacgttgtt ggtcatcaaa ccattcgaca cgactaacaa
    15901 ttctagggcg ctctccactt tgataaaata ttttgcctcg tagacgtccc atatcattaa
    15961 ttgtagccga attgttgtta cctttaattt cccaaaaagc tggtacagta acctgattaa
    16021 aaaatcgtgg tttcatattt tctgtattat gattatctgc aaaaaattga tacggtgata
    16081 taacatcgtc cggtaaaaag ccattgtcat tgagtacaat tgttaaatct tcttccaact
    16141 tactggcttt aaaagactca tataactttc gtgaatgatt gttaaagtaa tcaaataatt
    16201 taatcatgta gcacctcttg aactaatgtt tcccatttta aaataatatc ttgagtcata
    16261 aattgctgtg ccacttcata agagatgtca tgtggtgtct ggggaccatt gttaaaatac
    16321 attacaatgg catgagctag ttttgcgata acatcatcca cactatcttc atcggtatca
    16381 aaaggtacca agtagccatt ttccccatct cgaataaagg ttgggttacc ataattcaca
    16441 tttaatccaa tcatacctag tcctgagcct accgcttcca ttagtgttaa cccaaaacct
    16501 tcgctagttg atgcagaaag aaataactca taatcattat aaatttcatc aagtttaaca
    16561 tgccctagta aacgaatata atcttgcgcg cggtgtgtat caataatttt acgcagtcgc
    16621 gtcttctcgc taccttctcc ataaatatca aatgttaatt ctggcacttg tcgtttagcc
    16681 acgataaccg ctttgacaag ccaatcaata tgtttctcat ttgctaaacg agatgcacta
    16741 atcatcgcat atggctttct tgataattta ggatatgata atgcatcaat gcttcccaca
    16801 ggaatagtat aaacacgtgg gcgataacct tgatattgct caaattgtcg acaaaccata
    16861 tgattttgaa tatctgttgc tgtaataaag aaatcaatgt atttagcttt tgaaaattga
    16921 tattcataat aattgttcca tagtatatgc tgctcactca tcatattatt actataatga
    16981 tcagcatgaa tcacaacacc aactttacta tcacctttat gctgcaaaac agcctgacca
    17041 atatcagaag cgcggtctaa tatgacaata tcgtctcggg ttaaattcaa tcgttgtaaa
    17101 aagtatgcaa taaattcggt tttgttatac aacaccgcat cttcaaacac atatatagag
    17161 ctgtctccat caatatattc gttataagcg atggaaccat cttcattata gaattgtcgc
    17221 atatataatt tcgctttatt atcagctggt gcataatact cagaaaatat acgcgtataa
    17281 ctataaaaat ctttacgtac taacatacta ttaattacaa attctgcacg atccacaaca
    17341 tctttttgtt cattttgtag ataacatgtt acaaatgatg atttcccatt aaaatataga
    17401 cggactatct taccatttct ttctctaaaa ctaatttcat gaccaagctc acgttcaatg
    17461 tcatctaacg tgtacgttgt tggtgctata gaaatatcac taaaatactg atacaaccaa
    17521 ataacttctt gatctttaaa cccaatgttt tgcgttaatg tctgtatgtt ctctgactgt
    17581 ataaaatcta aaaacacaaa tttagtgtct tgatttgtac gtctcaataa tttagcacgg
    17641 taagcttgtg catattcaac accgctactc gcccagccta taccaaagtt tatattatat
    17701 attgtcatgc gctacccctt ttcatttatg gaaaatgtat aactggcata ccctctttat
    17761 caaatgtaat catgctttga caaatatttt tcaccattct ttttttgata tttcgtgtca
    17821 taacttcaaa tgaatctaat gcaactctat ggtattcaaa aatagcgtta cgttgcccat
    17881 tttgtcgttg attaacgctt gcttttaatt gttgtaaata gtcgacttgt tctaaccaac
    17941 atgaatcaat tgctttcaaa aagacttttt gaacgaaaat attataataa tatgcacttt
    18001 gcatgttttt acgattcaaa gctaattgct tttcaaattg ctctaataaa aatgtcacta
    18061 ctgcttgctt atctttaaaa ttaacacaag ccacatcttt attaaattgg aaacttaaat
    18121 tttgataaat atactcgaca acacgcgatt ttgttagcac cttttcctca tttacaaaca
    18181 tttcaaatac atctttagct aacgctttaa aatcttgatt ctcagcatca tctatttcta
    18241 aaactcgatt gcgttcctcg tatacaagat ctcgctgtat actaatgctt ttttcaaatt
    18301 cattagccat ttcacgagct ttaacccctt gttcttccga gatacgctgc gctttaacta
    18361 caatttgctt aactttgcga ttaaacaaat tactttgcga taatcgttgt gcatctaatg
    18421 aatataattg attattttcc gctaaattac tatcgctcca tcgcttaact aaataatcat
    18481 ctagtgaaat atatatacaa gatgatcccg gatccccttg tctaccagaa cgaccacgta
    18541 attgcctgtc tacacggcta ttttccatat gttcatgaat aataacagct aatccaccta
    18601 atgcttcgac accttcacca agtttaatat ctgtgcctcg acctgccata ctagtcgcaa
    18661 cagtcatgga accaatttgc cctgcttcag ctatcatctg cgcttctttt gcaacatttt
    18721 gcgcaatgag taaattatta ggaatatcca tttggaataa tactttcgaa aagtattcag
    18781 ccgcttcagc agttctcgtt atgagtaaaa ccggtcgccc cgtttcatga agttcaacta
    18841 tatcatgaat catcgcgatg tttttctcat caactgaacg aaacacttta tctggttcat
    18901 cgatacgttg aatcgcttta tcagttggta cttgtacgac tatttttgaa tacaaatcaa
    18961 agaactctga ttcgcctaat tttcctgtag ctgtcatacc tgaaaatgat tcaaaaagtt
    19021 taaataaatt ctggaaggta attgttgcca taacactttt atctgttgaa acctccatac
    19081 cttctttcgc ttcaatagct tggtgaagtc cagcttgcaa cttagttccc ggtaacatac
    19141 gacctgtaat acggtcaatt aaaacaatat caccattata tacaaagtaa tcgacattag
    19201 attcaaacaa atattgcgcg cgcagtgcta gattaatatt acgcactagg accatcgctt
    19261 gttcgctata taaatcttca acattaaagt atgattgtgc cgcttcaata ccttgattta
    19321 acagccatat ttcttttttg gtcttcttca ttttaaaatg cacgtcttca atcaatgtat
    19381 ctacaaactc tttcacaata tgaaatagat ttgattgtaa tcttggtgcg cccgaaataa
    19441 ctaatggtgt ttgcgcagca tctaaaatga ttgagtccac ttcatcaata ataccgtaat
    19501 ttaattgtgg taaaaatttc ccttccgcac tatcagccaa attatcaatt aaataatcaa
    19561 aaccgagacg tccattagtt gtatatataa tatcatgttc atataaatta cgtttttccc
    19621 ctttttgata ctcataatcc acaatatcaa caaaacctaa tgaagcagtt aaacctaacc
    19681 attcatataa tggttgcatc tcttcaaaat cacgcttcgc taaataatca ttcgttgtaa
    19741 ttaaatatgt tccttttccc gaaagtgcat ttaaatataa aggcatcgtt gctgttaatg
    19801 ttttaccttc gcctgtttgc atctccgcaa tgttaccttc atgcaataca atcgctccga
    19861 ttaactgaac ttctttagga tacataccta atactctcca gctcgcttca cgtgccactg
    19921 cataagcttc aggtaacaat gtatctagtg tatcaactcc tgatgctaaa cgttctttaa
    19981 attctattgt cttttgtttt aacgcatcat cagaatatga tttaacttca tcgccccatg
    20041 tattgatgcg tttcactatt tttctaatcg actttagtct taattcgttt atcgtaacat
    20101 ctagtttatg tttcatttac ttccccacca ttcagtttcg atacatctaa gtaatctaaa
    20161 aatcgtactg gattcattaa acgtgacata taatttagat ggttgtcttg ctcttcttta
    20221 aaataaacct cgacatttgt atcttttagt tcatgatttc ctgggacatg ttctttaagc
    20281 cattctttta aatcatcatc ttcatggctt gtacgatata ctttgcaatc taaatgctga
    20341 gcgacataag ttgcaaaaac atttgacttt gacccataac taatcaaatt aatagccgtt
    20401 agggtatctt gactttgcaa atcatccttt agttgcttaa tattttcctt gatatcgtct
    20461 tccatccaac gttcgacgag ccaaacatga ccaaacagtt tcaaaaaatc attcgaaata
    20521 gttggataga cgtcagatgg ttctgcaata atgacattta ttttatcatt tccatattgg
    20581 ttatcgtcta tcttcgttac ccgcatgctt ttatattcta aatcatcttg atgcgtcatc
    20641 tctgtgattg ttaaacatct aaatacaaga ctcgtcgatg ctgcattcat catttttatt
    20701 ttataagcat aggcttcatc aggatattga atcgtaatac tatttgactt tacaatctca
    20761 gtacttagtt ttgtgccatt tttattataa aaaatgatga taaaatacac tgaaccagca
    20821 ggcgttgcat caaaatcaaa atgcaattta taatgctgtc ctctacgcaa aattggtaaa
    20881 cttggcgcac ttttatattt tgaaaattgc tttaacatca accactcatg aatcggtaat
    20941 ccagagggca tcaaagggtt tataaaagtc acttcaccat ttgaaaatga tactttagag
    21001 ccatacataa atgtagtttg tgaaatataa ttccaagtaa ctttaaatgt tttgtttttc
    21061 agcatgttga actctcccaa acttgtcttc caaaataatg ttgtaaaaat taacaaacca
    21121 acttgcaatg gtaggtgaat catcattatg tcgcccagga atactgcgat tcatcactct
    21181 tgcttggtgt gctgtcaata caggtaatag ctcttgaaat gcatttggat cataatcatc
    21241 atgttgcata tatgctatgg caaaaacagt ttgtgacaat gatttctttt gaaatgtttg
    21301 ccaaaatttt tgatttaatg cctgtatcga cgcttgagat gtatcacctt cattagacac
    21361 caggacgtct aatgctgtac cgaactcttc tggtctaagt aatcgcatat gttcagcaat
    21421 cgttccaata ttaacaagtg gtttaccaac aataattgcc tgaggattta actgagcacc
    21481 ataatacaag gcaccaaatg aacccataga taaaccagat aatattaatt catgtgattt
    21541 aaaattcagc ttttctaatg tctcgtcaat aacattgata ataccttgtt catattcaga
    21601 tgaaccgata taaaaactac caccttcaac acgaggatcg ccgataagta aaaacggtgc
    21661 attcatacgt ttcatcatat aatatccttc gaaaccttcc gctgttcgat aaccactaaa
    21721 atatacgttt agtggtggtt tcatatcacc agggtggaaa taataaataa attcctgtcg
    21781 ttgactatct acgaaacgac taccaccgag taaaaattga cccatatcta atctagacca
    21841 tcgtttgtgt ataggtccta aatgtaccgt tccgttgcca cgcgccttaa cagttacact
    21901 tatataagca tcaaatggtt tcgtaggtat ctctaaagga ctgtctaaca tatcatcagt
    21961 caatacgatt tgttcaatta atgcaccatc agcgccagtc tgaatcaatc taaatgtata
    22021 ttgcaactcg accgcaccat caatatcaaa ttctggccat atttgaatga ctttatcttt
    22081 atcgtaaacg agattatttt gccaagatgc gataggttta aattcttttc caaattctcc
    22141 actcaatgtg agctctgaat taccttggta aacgacatct cctttaaaat tcggatgcac
    22201 aagtgctaac ttaggagaaa ccttatctcc atactgtcct gagaagctaa ctgcctctaa
    22261 tttattatta cgttcttcaa tattccggta atgtaatggt tgaacaacgt atttttggac
    22321 attttcgtct tgttcatatt caactgacca aaatgattca tcaacatacg tattgtatgg
    22381 ttcacttatc atttgtaata aattcgttaa tgtctccgag tatggtgctt gaatatagat
    22441 aaaatcaaag cgcccttctg cttcaacaat cgcttcaata gcctctacat aaccactatc
    22501 aaattcaaac aatccaatat cgaagtaatc ccaactcaca ccttttttgt gttgaaaaat
    22561 aggttctaaa tcgtctcctc caatttgcaa aactctaaat ttacgtggca tcattttcac
    22621 cttctattaa ctcatcgagc tgattaataa tattcttaga agcatatgca tctattaatt
    22681 ttaaagaata ggcgtacgca taattccaat ttttcaaata aaataaataa taatttaacg
    22741 catcatctaa ttcatcaact gtatttataa tacggccatt gtcataatca gagacgtaat
    22801 ctgtttgttg accattaatt tgtggaatcc cagcgctaat tgcactaatt tgtaaataca
    22861 agtcaggttc ttttgacata tctatcacaa gtcgcaacgt ccgcaatgct tctacaacat
    22921 catgttcagc atgtatcgtc ttaacagcaa tgatgtcatc ttgatcttca ggtgtcatta
    22981 atgctgaaac attaacatcc gcattctgtt tagcttggta ttcctcattt accgacgtaa
    23041 tacattcacg aagccacatc ggtatgtcat tttgatggcg cgataataaa attaaacggt
    23101 aataatcttc ctgtgcgata taatccacaa gtcgttgcat catttgttgc aaatcagcgt
    23161 cactcatacc atctatccat acacctataa atgtttccat caattgacta cttatattag
    23221 gtgattgtct cgtttcaaat ggtgtgattc gaatcattgt attctcccgc tgatacttct
    23281 cttgatgact aattaaatca cgttctagtt gcacactatc aacaattaaa tgggcattct
    23341 ttacgataga ttgatattcc tcatctgaca cagtttcatt tctattttta aaaaatgaat
    23401 aacttaatga tttcgctgga atatgattgg ctatttgtcg attgtgccta gcatctgaag
    23461 ccacaatcac atgatcatct tcatgtattt gttgtgcaat cattgcttga aatttttctt
    23521 caattagttg agccatattg ttatattctg tttgttgata gtgatgttga tatctttttg
    23581 aaacagtgac tctgccattt ttcaaatctt catgaagtac acaatctcca ttaatcgtta
    23641 aatattcttg gtaagaagcc tctccctgat catcaaaata acgtatcgct gataaataac
    23701 ctctgtcatc aaaaatataa cgccgttgta actgatctct ttcaaattct tcaaaccaaa
    23761 ttgaataccc ttcttgacta aaataaatat ttgtataggt ctgttcactc gtcacacatt
    23821 ttaataaata cggtgtgtac acaaactcaa catcatccgg ccattttaag tgatgataat
    23881 taatcgcttg tggcgcatgg tggctgaatc cttgaatttc atcaaacaca gacgaatact
    23941 ttgtctcata taagtcatat cgatgtaaaa atgttcttaa atttggtgca tgattgagaa
    24001 caatcagttg ataatctaag tcattttcaa ggtgcattcc cattaaacta atcatatcgt
    24061 caaattccgt cttattttgt agttgataat acggcacagt cgtgtcttgc caccatcgtt
    24121 ggtcatcgta ccaagctgga ataaagtatt tcataattac ctccttacca atactggctt
    24181 aaaaatggct tatatttatc aaaatataaa tatgtacgaa ttgtttctgc aatattaata
    24241 ctgatgtaaa ctaatacaat tagttgtact gagaaataaa tttcagtaga taaatgcggt
    24301 acaaacaatg tgaaataaag cggtatacca ataatgattg taactaatgc cgatccaaac
    24361 caacatacgc gtcgtgcttg ataatttaaa taacgttctg tatccttacc aggtttaact
    24421 cctgaaaaat aattgccact ctttaagaaa tctttggatt tttgtttagt attgattaaa
    24481 aatctcgata aaaaataacc caataacatt tgaatcacta aatacaccga aataccaatt
    24541 ggactatcaa atgtcagcat tggcatgtca tctgatatgc ttttattaaa catagataaa
    24601 ataaaatgaa tgccactttt taagaaaaca aaagctgaaa tgctcatcat taaagtaata
    24661 ctgcctgcag ggttaacttt ccaagataaa taagatttca tatttgttgc ggaaacgttc
    24721 attaaatcga tatatggtat tctcacttct actaattcaa taaataataa gataaacaat
    24781 gtgattatca caaggatgat taacaacgca atcacaatat gacttgcatc tatatattcc
    24841 attttttgat gcatcattga tttaataatg ctaaccatta caatcggcat tggtcctgca
    24901 atgccgtaac gactattttt gtcagctaac caaactaata acatcgttcc agtaaccaaa
    24961 atcaatattg ttaagtaaat attgtcttga tgaacacgtt ctttcgaaac atattcatga
    25021 atcacaaaat aactttgaat aacacttaaa attaatgtta agatgcgctc tttataatgc
    25081 ttctcaagac ttgtttgttt catgtattta tccatattcc gataagaaat cagcatcaag
    25141 ataatcattg atgttaacca cggacctaat cctaaagtga aaatgtttaa agtattaacg
    25201 tctccaccca tattagaaat agctatttta aaaaatgact catgttttac ttgcatatcg
    25261 ttataggaaa cgatggaaat gtttgtgcct aatatataaa taaacaagat aaaacatgtg
    25321 tatagcatac gtttatatat aattttatat tcgtattgtt gtaaaagttt taacatgttg
    25381 cacctctttt atatcaaaaa cattaaaaag actaagggtt catcactaat tattaaaatc
    25441 ctatatcgat ttttctagtg attggtgcct cagtcttttt aattttagcc agctataaat
    25501 tcaatttatg cttgagaatc atcttgatca ttttcatctt tctttttctt tctcttcatt
    25561 aaacctaaac caactaataa tgtcataacg ccacctagta atccattttg ttttattgag
    25621 tcacctgtat ctggcaatct tttttcactt tgtgctggcg tgccattatg tttagtcact
    25681 tcagatgttg cacttaatgt agactgagat tcactcgtgc tcgttgttgc ttcacttgat
    25741 aagcgagatg tgctcgtgct gtgagtatga tgcatactca ttgagtctga cggatgcatt
    25801 gagttagatt cagatgtact tgttgagccg gacatacttg ttgatgtcga gtcagaaatg
    25861 ctttgtgaac cagacataga tgtactcagt gattcggatg tgcttgtcga atcggatgtg
    25921 ctcaatgacg ttgatgtgct tgttgacact gattctgagt cgctcactga agtcgagttg
    25981 tacgttgaac cggacatact ttgtgacatt gaaacacttg ttgaatcgga ttcactcatt
    26041 gatgttgagt cagatacgct cgttgaacct gaaccagacg tacttaatga ttcagatatg
    26101 cttgttgaag ttgaaccact tgttgagtcc gatgtacttg tcgatgtcga gtctgaatct
    26161 gatgtactca atgattctga gtcactgata gaagttgaat cacttgtaga ttctgattct
    26221 actgtacttt gtgaaccact gatacttatt gaagtagaat cactgatact gtctgatgtt
    26281 gataatgatg tcgacaccga tgtgctttgt gatgacgatg tactagcact cattgacatt
    26341 gatgttgata tcgatgtact taaggaacca gatgcacttg tacttgttga ctggctttgt
    26401 gacattgaat cacttaatga tgtagatgtg cttgttgagc tcgagtcact taaacttgtt
    26461 gaacctgata ttgagtcact taaacttgtc gatgttgaaa ctgatacgct tccgctcatt
    26521 gagtcagatg ttgaaagtga tgtactcgtt gaatttgatc cactgatgct agacgaatca
    26581 cttgtagaca ttgagtcgct ttctgatgca ctgatgctca tagagtcaaa ttgactatta
    26641 cttgttgagc ttgactgcga atcgctcaca cttgttgacg ttgattctga tccactcata
    26701 ctttgcgagc tactcaatga ttttgaatca cttaatgaat ccgaagtgct aagacttgtg
    26761 gaaccactta aagatattga tccacttaat gagtcggagt cacttgtact agtagaatca
    26821 ctcattgata ttgaatcact tagcgaggta gactcgctta cgctttctga accacttaat
    26881 gatgttgagg tactcaatga accagatgta cttgttgaag tcgaaccact tgtcgatttt
    26941 gaatcactta atgaatcaga ttcactcacg ctttccgaac ttcttagtga cgtcgataca
    27001 cttaatgatg acgaatcgct tgtgcttact gaatcgctca tcgattgtga gccactcaat
    27061 gaactagact cgcttacact ttctgatttt cttaatgacg ttgagacgct caatgagcca
    27121 gaatcactta cgcttgttga accactcatc gattttgagt cactttcaga attagattcg
    27181 cttacacttt ctgaatcatt tacagattct gacatacttt gtgaatcaga tatgcttgcg
    27241 ctcattactt cactagccga tgttgatgtg cttgtcgaat cacttaacga tatagacaca
    27301 ctcatcgaac cagatgtact cgcacttgtt gagtctgatg ttgaatcact aacactatca
    27361 gataatgacg ttgaatcact catacttgtt gatgtacttg ttgaaagcga catactttgt
    27421 gaatcacttg tacttgtacg catcgaagta ctagttgaag ctgatgtact acgagagtca
    27481 cttgttgatg ttgatgtact tgctgatcca gatgcacttg tacttcttga tgtgctttgt
    27541 gaatcgctta atgatgttga tgttgaatcg gattcacttg tactttctga tgttgagcca
    27601 gattctgatg tacttaccga tgttgataaa cttgcaatgg tcgacatgcg gtttgaagtt
    27661 gatgtactta gcgaatcact taatgatgct gatgtgcttt gtgaatcgga ttcactgctt
    27721 tgcattgacg cagatgttga atcacttaaa gatgttgacg acgaatcgga ttcacttgcg
    27781 cttgctgagt tactgagcga tgttgacgta cttgctgatc ctgagttact tgtacttgtt
    27841 gagtcactga gtgatgttga cgcactttct gatgttgatt cacttgcact tgttgagata
    27901 ctttgtgaat cagattcact ctctgaacca cttaatgacg ctgatgtgct taccgaacct
    27961 gattgactcg tacttttaga tatacttgtt gaatcgctta gtgacgttga tgtcgatgtg
    28021 gattcacttt gacttgttga ctgacttgta tatgatgatg tgctttgtga atcggattcg
    28081 ctcgtgcttg tacttgttga gtttgaggca ctttggcttg ctgagtttga gtctactccg
    28141 ctttgattca ttgatgcact tagtgacaat gacgtactcg ttgagtcaga caaacttgta
    28201 ctcgttgacg tacttgtact tcctgatgtt gattgagaca tacttatgct cattgatgtt
    28261 gaatcggatt tactttcact tgatgatgtt gagtcggatt cactttcact tgtagaacca
    28321 cttaatgatg tggatgtact aatggaatca gatttacttg tactgattga atcacttgtc
    28381 gacattgatg tacttaatga atcagactta ctatcacttg tggaatcact taatgatgtt
    28441 gacaaacttg tagagtcaga caaacttgta ctcgttgaca tacttagtga tgttgaaaca
    28501 ctctcgctct taaacgttga cgttgattca ctgatacttg tcgatgttga aatggacgca
    28561 ctaccacttg ttgaattact taatgatgtt gatgtgctac cagattctga tgtactgtct
    28621 gataatgacg tactctcact tgtcgaacta ctcactgact ctgatgttga ttcagacgta
    28681 ctttcactta atgattcact taaaaaggca gatgcacttt gtgattctga atcgctagta
    28741 ctatttgatt cacttaatga tataaacgtg ctctctgaag cagatattgc ttcacttata
    28801 gagtcgctcg ttgacgttga ttcacttatt gaatcagact ctgatgtact taagcttgtg
    28861 gaatcactca tagatgttga tgtacgttct gaattacttg tacttaacga tgtcgaagta
    28921 cttaccgaac cagatgtgct cgtagaagca ctttgtgata ttgattcact tgatgcagtt
    28981 gatgctgatt tgctatcact cgttgaatca ctaaacgacg ttgatatgct cattgaatcg
    29041 gattgacttg cactcattga accagacgta ctttgtgatt ccgaagtacg tactgaagca
    29101 cttgtcgacg ttgatgtact tgttgaacct gattgactag tacttagtga taatgactta
    29161 ctatcagaat cagatgtact ttgtgaatca cttaatgatt ctgatgtact acctgactga
    29221 gacgtgctca ttgaactact tacagacatt gatttactgt ctgatgcaga taatgaccca
    29281 cttgtactga tagagtcact tactatctct gaagtactca tcgagtctga tgtacttgtt
    29341 gagacacttt gtgatgctgc tatgcttagt gatccagaaa cagaaccact tgtgctcgtc
    29401 gaatcgctca atgattctga tgtactcatc gattttgaat cacttgtact taatgatatt
    29461 gatgtacttt gtgaatctga tttgcttgtt gacgcacttt gagagttggc tatgctattt
    29521 gaaatactga tagagtccga ggtgctagct gactcgctca atgattctga tgtgctgatt
    29581 gcattcgatg tgctgtcact taatgatgct gatgtactag acgattctga tgtacttgtt
    29641 gatagacttt gtgacttaga caagctcccc gatgtactca tacttaatga gtcactgagt
    29701 gacgttgatg tacgcaatga atcagatgta cttgttgata gactttcgga tttttcagta
    29761 ctgctagagt ttgaaataga atcgcttaat gatgttgatt tactagctga atccgacatg
    29821 cttgatgata cactttgtga attcactaaa cttgtgcttg ttgagcttga tacactatta
    29881 ctttcagatg tgcttaatga cttagatgca ctcacagaat cagataggct tacacttgtc
    29941 gatttcgagg tactagctga tgtagatacc acaatcgatc ctgatgtact cgttgatgca
    30001 ctttgtgagt cagctttact tgttgagaca ctttgggatt gttgtgtact tcctgatgtt
    30061 gatacggaat cactcatgct atttcttgtt acttcatatt taaaagttgt tgtcgttttg
    30121 ttaccactcg catctgtaga aacgattgta atagtacttg tatcaatgtt tgttggtgta
    30181 ccactaatgg tattatttgt actatcgaat gttagtccag atggcaatcc agtcactgta
    30241 ttcgtcaccg catttccact gttgtcctgc gtagcaattt taatcggtga tattggcgaa
    30301 tacacttctg atgattgatc tcctattggt gtcactgttg gtgctgtcgt atccacaaca
    30361 tttattgtaa aagttgtcgt cgatttattg tttgcttggt cagtagacac aactgtcact
    30421 gttgattgac caattttcgt tggcgtccca atgattgaat tcgttgcact atcataactt
    30481 aatccgcttg gtaatcctgt aactgtattt gtcacagtcc cagtaccatt atccgttgta
    30541 gtcaacacaa caggattcat tgttttaccc acttcaatgg tttgattgcc tacagttaca
    30601 gttggtgctt ttacatcagt aaaataatat gtcactgatt gtccagcatt cgtcattttt
    30661 acagttttat ttgtatcatt ataagttgac gcatatgaac tatcgacgga cgtgtagtta
    30721 tatcctttag cagtcaatgc agattgctga ttatcgattg tcacgacttg atcaacattt
    30781 cctgaatatg tttttggtgg aataatatct ttacctgttg ttacatcaac gtatctcact
    30841 tgtgtaacag cagactctgt atattcgaat gttccaaatt gtacttgttg taaatttgtc
    30901 gcgccacctg ttgaggctgt cattgataat gaaaagttgg tcgtaccact ttttgcaatc
    30961 caatctgaaa tattacgtgt ccatgtttga cctgcatacg ttacagtcat aacttttgta
    31021 tcaccattat aattaatatc aaaatcttgg aacgtgttgt tcgtaggttg cacttttaac
    31081 ttcgcagcat tatcagctgt tgaacttgat gtatacgttg tcgcaacacc ataactatct
    31141 gttgttacaa aggcaccaaa cgcacctcca ccagctacat tagatgggtc agcattcgcc
    31201 tttgcagctg aatttggttt agatgtattg tgatacgtat ccaatttgaa gccaaatgcg
    31261 ttacttaagc caccaatacc tactgcggca ccgtttaacc ctgtttcacc taatacacct
    31321 ggcgaaaagg caaagccgat accatctcca ccatttccat taccttcgta tttattacct
    31381 aaatttactt ttccagaaaa atgaaaactc ttattagagt caatacgtgt tcctaatgta
    31441 atggcacctt tttggctgta tgcatcctgt gttaacgtca caataccggt actttgatca
    31501 taggtagcat tacctgacgt tgtcatatat tgttttaagt tatctttatt aactgtaatt
    31561 gtattagcag ttactgcggt tgtcgtcgct gctgacgcaa atgttgacat agctaagcga
    31621 ctgaaagttc gaagttttac tggtgcggtg ctagttgacg ttgtgctagt tttgtttaag
    31681 ttgaccgaag atggcgttgt gctttgtgaa gtgttatttg atgcagtact ttgatttgtt
    31741 gatgtattaa ttggttgttc tgtacttgaa gttgaagcta cagatttagt atcagaactt
    31801 gatgtagtat tctttgagga tgttgattct gatgtagatg tcaatttttc ttgttgattg
    31861 cttgtactat tagttgtcga agtgaccttt tcagactttt cacttgagac tgtgtcacta
    31921 tttgatgttt gtaccgaact actatttttc gttacacttg tggaatcggc tgttgatgtt
    31981 gatgcttcga ttgtcgttga gttttgatta cctactgttt cactttgtgt gtttaattca
    32041 gaagttaatg gtgcatcaga agccgcaaaa gcttgctggt catgcaacat atttaccgtg
    32101 aatgcaccac caataaccgc cgtagttttc agtccgtatc ccgtcatttt tttactaatg
    32161 ctttgattat cttgactcac taaactatga ctaataaatg gtagccccat aattttgaac
    32221 atttctattt ctttaattcc ggattttacc caattttttc cagatttata aagtcttact
    32281 cttgtttttt cgtttgctaa gctgtcatga aattctttct gtcttttact catgtaataa
    32341 ctccttgtat tatctttaca ttcattagat tataatatat gccactattc aatttaatac
    32401 aactcttttt tgatacaaaa atactcattt tgttaaaatt tgtaaaaatt catttttatt
    32461 cgtctaaatg taatcgtttt catattttta aaattacttt ttctcgttta tgcgtataat
    32521 ctttttttat ataaatttgg ctaattggct ttatgtttaa tcattataat tgtttcgttt
    32581 t
//
LOCUS       DAMZLE010000015        30131 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMZLE010000015 DAMZLE010000000
VERSION     DAMZLE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.015"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(53..214)
                     /locus_tag="POB92_000683"
     CDS             complement(53..214)
                     /locus_tag="POB92_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578203.1"
                     /translation="MLLILELRFWNSEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(251..991)
                     /locus_tag="POB92_000684"
     CDS             complement(251..991)
                     /locus_tag="POB92_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI6578204.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRSLNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDRDATLVIHDDMVSVVKSLMTE"
     gene            1197..1988
                     /locus_tag="POB92_000685"
     CDS             1197..1988
                     /locus_tag="POB92_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6578205.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2356..2784
                     /gene="lacA"
                     /locus_tag="POB92_000686"
     CDS             2356..2784
                     /gene="lacA"
                     /locus_tag="POB92_000686"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDI6578206.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2800..3315
                     /gene="lacB"
                     /locus_tag="POB92_000687"
     CDS             2800..3315
                     /gene="lacB"
                     /locus_tag="POB92_000687"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDI6578207.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3328..4260
                     /gene="lacC"
                     /locus_tag="POB92_000688"
     CDS             3328..4260
                     /gene="lacC"
                     /locus_tag="POB92_000688"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDI6578208.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4264..5244
                     /gene="lacD"
                     /locus_tag="POB92_000689"
     CDS             4264..5244
                     /gene="lacD"
                     /locus_tag="POB92_000689"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDI6578209.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKNQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5266..5577
                     /locus_tag="POB92_000690"
     CDS             5266..5577
                     /locus_tag="POB92_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI6578210.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5583..7295
                     /locus_tag="POB92_000691"
     CDS             5583..7295
                     /locus_tag="POB92_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6578211.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7313..8725
                     /gene="lacG"
                     /locus_tag="POB92_000692"
     CDS             7313..8725
                     /gene="lacG"
                     /locus_tag="POB92_000692"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDI6578212.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            8991..9860
                     /locus_tag="POB92_000693"
     CDS             8991..9860
                     /locus_tag="POB92_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6578213.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10143..11144
                     /locus_tag="POB92_000694"
     CDS             10143..11144
                     /locus_tag="POB92_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDI6578214.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEETDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTGTLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11378..12385
                     /locus_tag="POB92_000695"
     CDS             11378..12385
                     /locus_tag="POB92_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI6578215.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            12693..14255
                     /locus_tag="POB92_000696"
     CDS             12693..14255
                     /locus_tag="POB92_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6578216.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            14430..14966
                     /gene="amaP"
                     /locus_tag="POB92_000697"
     CDS             14430..14966
                     /gene="amaP"
                     /locus_tag="POB92_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDI6578217.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            14979..15218
                     /locus_tag="POB92_000698"
     CDS             14979..15218
                     /locus_tag="POB92_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDI6578218.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15281..15790
                     /locus_tag="POB92_000699"
     CDS             15281..15790
                     /locus_tag="POB92_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6578219.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(15950..17926)
                     /gene="sfaD"
                     /locus_tag="POB92_000700"
                     /gene_synonym="sfnaD"
     CDS             complement(15950..17926)
                     /gene="sfaD"
                     /locus_tag="POB92_000700"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDI6578220.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLGRTMSLSKDYDTTVHQLFPELIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVTGI
                     HFYNEQDEVDYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSNFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKVQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            18028..19221
                     /gene="sfaA"
                     /locus_tag="POB92_000701"
     CDS             18028..19221
                     /gene="sfaA"
                     /locus_tag="POB92_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDI6578221.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPRVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            19208..20965
                     /gene="sfaB"
                     /locus_tag="POB92_000702"
     CDS             19208..20965
                     /gene="sfaB"
                     /locus_tag="POB92_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDI6578222.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEIVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEEAELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            20972..22042
                     /gene="sfaC"
                     /locus_tag="POB92_000703"
     CDS             20972..22042
                     /gene="sfaC"
                     /locus_tag="POB92_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDI6578223.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(22143..22268)
                     /locus_tag="POB92_000704"
     CDS             complement(22143..22268)
                     /locus_tag="POB92_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578224.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            22434..23417
                     /locus_tag="POB92_000705"
     CDS             22434..23417
                     /locus_tag="POB92_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6578225.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVNPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            23429..24460
                     /locus_tag="POB92_000706"
     CDS             23429..24460
                     /locus_tag="POB92_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6578226.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            24457..25425
                     /locus_tag="POB92_000707"
     CDS             24457..25425
                     /locus_tag="POB92_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6578227.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            25661..27031
                     /locus_tag="POB92_000708"
     CDS             25661..27031
                     /locus_tag="POB92_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDI6578228.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(27163..27423)
                     /locus_tag="POB92_000709"
     CDS             complement(27163..27423)
                     /locus_tag="POB92_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578229.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            27600..28115
                     /locus_tag="POB92_000710"
     CDS             27600..28115
                     /locus_tag="POB92_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6578230.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPRGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            28159..29346
                     /locus_tag="POB92_000711"
     CDS             28159..29346
                     /locus_tag="POB92_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDI6578231.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            29365..30048
                     /locus_tag="POB92_000712"
     CDS             29365..30048
                     /locus_tag="POB92_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI6578232.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
BASE COUNT        10323 a         4316 c         5631 g         9861 t
ORIGIN      
        1 tgtttatttt taatcttaca aatgacaaac cattaaaagt agcatcccaa catcaaacaa
       61 aaaaacaatt catcaaataa aaatcgctac aaaaccaagt cattaaacac gcaataatta
      121 aaattttcca ctcattataa ttctgaattc caaatgtcga attccgaaaa ccaaactccg
      181 aattccaaaa acgcaactcc aaaattaaaa gcatctccct gttactcggg aaatgctttt
      241 tacctactga ttactctgtc attaatgatt ttacaacgga aaccatgtcg tcatgtatga
      301 ccaaagtagc gtctctatca taaggtgttc gatctttatt gataataatt aaattgtcgc
      361 ctttaaaatt tgatattaat cctgcggcag gttgtacaac gagtgatgaa cctagtacaa
      421 caagggtgtc agcatgttca attttattta atgaccgtat gatggtaggt tgatctaaca
      481 tttcaccgta taatacgatg tccggtcgaa tggcaccacc acaattatca caatgtttca
      541 aagttctatc aatgacatct gacttcgtat aagatttatg acatgcatta caataaaaac
      601 gatttaacgt gccatgtaat tcatcaacat gttgacttcc agcgtctgag tgcaaaccat
      661 cgatattttg cgtgatgaca cctaaagatt gttgattacg ttctaatttt gcaatccaat
      721 catgaacgat attaggcttc gtatcgacaa atagtaaacg cttatggcag aaattgataa
      781 aaccttcagg atcatcttct aaataatcac ggcttaacaa gtattctggc gaaagtccat
      841 cttttgaaat ttcatcaaat aagccaccca ttgaacggaa atctggaacg ccacttgcga
      901 cagatacacc agcacctgtg aaaaatgtaa tacgattcga actatctata atatgtttta
      961 atgtctctaa atcatttctc aaaataaaca ccccatcaaa ataatgaatg atttataacg
     1021 tcttttgctt ttattatagt atattcattt tacgattatc aaaagcgcag tttaaacagt
     1081 tctttttaaa aatgaattat acatataatc gaacagttaa attatattag tttttacgca
     1141 atttttatta tagaataagc atgtatatta aaattgatgt ctaaacataa aggataatga
     1201 cccatcgcct aaataaagga ggcaatttta cattgaataa acatgaacgt ttggacgaaa
     1261 ttgctaaact agtgaataaa aagggcacga taagaacgaa tgaaatcgtc gaaggtttaa
     1321 atgtgtctga tatgacagtt cgaagagatt taattgagtt ggaaaataaa gggattttaa
     1381 cgaagattca tggtggtgca cgcagtaatt caacatttca gtataaagaa atatcgcata
     1441 aagaaaaaca tacccgtcaa attgcagaaa aacgatttat agctaaaaaa gctgcatcat
     1501 taattgaaga tggggatact ttgtttttcg gaccgggaac aacagtggaa ctattagcgg
     1561 aagaagtcaa tcatcatacg ctcacaatta ttacgaattg tttgccagtg tataaaattt
     1621 tgttggaaaa acaaacagca cattttcgtg tctatttaat tggtggtgaa atgcgccata
     1681 ttacagaagc gtttgtaggt gaaatggcga atgccatgtt agaaaaacta agatttagca
     1741 agatgttctt tagtagtaac gcagtaaata aaggtgctgt gatgacatct acattagatg
     1801 aagcctatac gcaacaattg gcactaagta attcaattga aaaatacttg ttaatcgatc
     1861 atacgaaagt tggcaaagaa gattttacat cattttgtca gttaaacgaa ttgactgcag
     1921 tggtcatgga ctatgaagat gaagaaaaag tagaaacgat taaaacatat attgaagtag
     1981 tcgactaaat taaaagtata actgcattgt taaaatatgt atctatatgc tcataagtga
     2041 atgtgaattt gcattcattt atgggctatt ttttatgcaa atttatgtaa acgggaacct
     2101 cgacaaagtt ttactacaat ttcagacgaa tacctaatta aaacaacacc acagtatgaa
     2161 tgttcactta ttgtttgaaa atatgtcata aacaaacaga ttataaacat attatgttga
     2221 tttattgttt gtttatgttt ataatttaaa cgtaatcaaa ttgaaagcct tttctcaaga
     2281 ttttcataac ctacaaataa acatgtcata ttttaagaaa ggtatttcaa aattaaagta
     2341 aaaaggagtc ttattatggc gattattatt ggttcagatg aagctggcaa acgattaaaa
     2401 gaagtcatca aatcatactt attagacaat aaatatgatg ttgttgacgt aacagaagga
     2461 caggaagttg actttgttga tgcaactttg gctgtagcaa aagatgttca aagtcaagaa
     2521 ggtaacttag gtattgttat cgatgcattt ggtgcaggta gctttatggt tgctacaaaa
     2581 atcaaaggca tgattgctgc tgaagtatca gatgaacgct ctggatatat gactagaggc
     2641 cataacaatt caagaatgat cactatgggg tctgaaattg ttggagacac attagcgaaa
     2701 aatgtcgtta aaggttttgt tgaaggtaaa tacgatggtg gtagacacca aatccgtgta
     2761 gatatgctta ataaaatgtg ctagggagga aaaattacta tgaagattgc attaggatgc
     2821 gaccatattg ttacagatac aaaaatgcgt gtatctgaat ttttaaaatc aaaaggacat
     2881 gaagtcattg acgtaggaac atacgatttc acaagaacac attatccaat ttttggtaaa
     2941 aaagttggcg aacaagttgt tagcggtaat gcagacttag gtgtttgtat ttgtggaaca
     3001 ggtgttggta ttaacaatgc tgtaaataaa gtacctggcg ttcgttcagc actagtacgt
     3061 gatatgacat cagcgttata tgctaaagaa gaattaaatg cgaacgttat tggctttggt
     3121 ggacgtatta taggtgagtt attaatgtgc gatattatcg atgcatttat taacgctgaa
     3181 tataaaccaa ctgaagagaa taaaaaatta atcgctaaaa ttaaacattt agaaacaagc
     3241 aatgcagatc aagctgatcc acatttcttt gatgaattct tagaaaaatg ggacagaggc
     3301 gaataccacg attaagggac gacacatatg attttaactt taacattaaa tccatccgtt
     3361 gatatttcat atccattaac agcattgaaa ttagatgatg ttaatcgtgt tcaagaagta
     3421 agtaaaacag ctggtggtaa aggtttaaat gtaacaagag tacttgcgca agtaggcgag
     3481 ccagtccttg cgagtggttt tattggcggt gaattaggtc aatttattgc taaaaaatta
     3541 gatcatgccg acatcaagca tgcattttat aacattaagg gtgaaacacg aaactgcatt
     3601 gcaattttac atgaagggca acaaacagaa attttagagc agggaccgga gattgataat
     3661 caagaggctg caggatttat taaacatttt gaacaattat tagaaaaagt tgaagcagtt
     3721 gctatttcag gtagcttacc taaaggatta aatcaagatt attatgcaca aattattgaa
     3781 cgatgccaaa acaaaggtgt gcctgtgata ttggactgct cgggtgcaac tttgcaaaca
     3841 gttttggaaa atccatacaa accaacagtc attaaaccaa acatttctga actatatcaa
     3901 ttgcttaacc agccacttga tgaaagttta gaaagtttga aacaggcagt gtcacaaccc
     3961 ttattcgaag ggattgagtg gattattgtg tctttaggtg cacaaggtgc attcgctaag
     4021 cacaatcata cattctatag agtgaatatt ccgacaataa gcgtattaaa tccggttggt
     4081 tcaggtgatt ctacagttgc tggaattact tcagcaattc tcaatcatga aaacgaccac
     4141 gatttactaa aaaaagccaa tactttaggc atgctgaatg cgcaagaagc acaaacaggt
     4201 tatgtcaatt taaataatta tgatgattta ttcaatcaaa ttgaagtttt agaggtgtaa
     4261 aaaatgtcaa aatcgaatca aaaaatcgca tcaattgaac aactaagtaa taacgaaggt
     4321 atcatttctg cattagcatt tgaccaacgt ggtgctttaa aacgcatgat ggcaaaacat
     4381 caaactgaag agccaactgt agctcaaatt gaacaattaa aagtattagt tgcggaagaa
     4441 ttaacacaat atgcatcttc aattttatta gatccagaat acggtttacc agcatctgat
     4501 gcacgtaaca aagattgtgg attattgcta gcttatgaaa aaactggtta tgacgtaaat
     4561 gctaaaggtc gtttacctga ctgtttagta gagtggtctg caaaacgttt aaaagaacaa
     4621 ggtgcaaatg ctgttaaatt cttactttac tatgatgttg atgatgctga agaaattaac
     4681 attcaaaaga aagcatacat tgaaagaatc ggttcagaat gtgttgcaga agacattcct
     4741 ttcttcttag aagtattaac atacgatgac aacattcctg ataacggtag tgttgaattc
     4801 gcaaaagtta agcctagaaa ggttaacgaa gcaatgaaat tattctcaga accacgcttt
     4861 aatgttgatg tattgaaagt tgaagtacca gtaaacatga agtatgtaga aggctttgct
     4921 gaaggtgaag tggtttatac aaaagaggaa gcggcacaac actttaaaaa tcaagatgca
     4981 gcaactcatt taccatacat ttacttaagt gctggtgttt ctgcggaatt attccaagaa
     5041 actttaaaat ttgctcacga agctggtgct aaattcaacg gtgtactttg tggccgtgct
     5101 acatggtctg gtgctgttca agtatacatt gaacaaggtg aagatgctgc tcgtgaatgg
     5161 ttacgtacaa ctggtttcaa aaatattgat gacttaaata aagtattaaa agatactgcg
     5221 acatcttgga aacaaagaaa ataagtataa aaggggatta atgctatgaa tagagaagaa
     5281 gtccaattat taggttttga aattgttgca tttgcagggg atgcacgttc taagttttta
     5341 gaagcattga cagcagctca agctggagat tttgcaaaag cagatgcatt gattgaagaa
     5401 ggaaacaatt gcattgctga agcgcataga gcacaaacaa gcctgttagc taaagaagca
     5461 caaggtgatg atattgcata cagtgtaacg atgatgcatg gtcaagacca tttaatgaca
     5521 acaattttac tgaaagattt aatgaagcat ttattagaat tttataaaag agggtgatga
     5581 caatgatgca aaaattgatc gcacaaattg aaaaagggaa gccatttttc gagaaattat
     5641 cacgtaacat atatttaaga gccatccgtg atggatttat ttctgcaatg cctgtcatct
     5701 tattctcaag tatctttcta ttaattgcat acgtgccaaa tatatttggt tttaaatggg
     5761 ataaaggcat ggaagccatt ttaatgaaac catataacta tacgatgggg cttgtagcat
     5821 ttttagtagc aggaacgaca gcgaaatctt taacagattc atttaaccgt aagctagaaa
     5881 gtacaaatca aattaacttt atttctacaa tgctagcagc gatgtgtgga ttcttgttct
     5941 tagcttctga tccagccaaa gatggtggat tcttaagtgc atttatggga actaaaggtt
     6001 tattaacagc atttttatca gcatttgtaa ctgttattgt ttataacttc tgtgtgaagc
     6061 gcaatattac aattaaaatg ccgaaagaag taccgccgaa tatttcacaa gtatttaagg
     6121 acttaattcc attttcagcg gtaatcatca ttctttatgc attagattta gtcattcgca
     6181 acagctttaa atcaaatgta gcggaaggta ttttaaaatt attcgaacca ttatttacag
     6241 cagcagatgg atggattggt gtcacaatta tctttggtgc ctttgcatta ttctggtttg
     6301 taggaattca tggtccatca atagtagagc cagcaattgc agccattaca tatgcgaata
     6361 tcgaagcgaa cttcaagttg cttcaagctg gagaacacgc agataaaatt attacatcag
     6421 gtacacaaat gtttatcgtt acttttggag gtacgggtgc aacattggtc gtcccgttca
     6481 tgtttatgtg gatgacgaaa tctaaacgaa acaaagcgat tggacgtgca tcagttgtac
     6541 caacattctt tggtgtaaac gaaccaattt tatttggtgc accactagta ttgaatcctg
     6601 tgttctttat tccatttgta ttagcaccaa ttgttaacgt atggattttc aaacttttcg
     6661 ttgaagtgtt aggaatgaat agttttagtg tgaatttacc ttggacaaca ccaggtccat
     6721 tgggcattat catgggtaca ggttttggtt tatggtcatt cgttctagct attactttga
     6781 ttgttgtaga tattattatt tactacccat tcctaaaagt ttatgatagt gaaattcttg
     6841 atgaagaaga aggacgtaaa gaaagtaatt cagatttaaa agaaaaagtt gcagcaaact
     6901 ttgatacgaa aaaagctgat tcaattttag cggcaagtgg tgtatcagac gatgcagcta
     6961 aagcttcaaa tatcacagaa caaacaaatg tcttagtact ttgtgccggt ggtggtacaa
     7021 gtggattatt agccaatgca cttaataaag cagctgaaga atatcatgtg ccagtgaaag
     7081 cggcagctgg cggatatggc gcacatatgg acattatgaa agagtaccaa ttaatcattt
     7141 tagcgccaca agttgcgtct aactatgaag atattaagca agatacagat agattaggta
     7201 ttaaattagc gaagactcaa ggtgccgaat atatcaaatt gacacgagac ggccaagcag
     7261 cccttgattt cgttcaacaa caatttgaaa attaagaata ggagtttttc atatgactaa
     7321 aacattacct gaagatttta tttttggtgg agcaacagca gcatatcaag cagaaggtgc
     7381 gacaaatacg gatggtaaag gtcgtgtagc atgggatacg tatttggaag aaaactattg
     7441 gtatactgca gaaccagcga gtgattttta taacagatat ccagtggatt tagaattaag
     7501 tgaaaaattc ggtgttaatg gtatccgtat ttcaattgca tggtcacgta tcttcccaaa
     7561 tggatatggc gaagttaacc ctaaaggtgt tgagtattac cataagttat ttgcagagtg
     7621 tcataaacgt catgttgaac catttgtaac attgcatcat tttgatacac cagaagtgtt
     7681 acacaaagat ggcgattttc taaatcgtaa aacgattgac tactttgtag attatgctga
     7741 atattgtttt aaagaattcc cagaagtaaa atattggact acattcaatg aaattggacc
     7801 tattggtgat ggccaatatt tagtaggtaa attccctcca ggtattaaat atgattttga
     7861 aaaagttttc caatctcatc ataatatgat ggttgctcat gcaagagcag tgaaattatt
     7921 caaggatggc ggctatcaag gagaaattgg tgttgtacac gcattgccaa caaaatatcc
     7981 gttcgatcca tcaaatcctg aagatgttcg tgcagcagaa ttagaagata tcatccataa
     8041 taaattcatc ttagatgcaa catatttagg taagtattct cgtgaaacga tggaaggtgt
     8101 gcaacatatc ttatctgtta atggcggtaa attaaatatt acagatgaag attatgccat
     8161 tttagatgca gccaaagatt taaacgactt cttaggtatc aattactaca tgagtgactg
     8221 gatgagaggt tacgatggag aatctgaaat cacgcataat gcgacaggtg ataaaggcgg
     8281 ttctaaatac caacttaaag gtgtcggaca acgtgaattt gatgtcgacg tacctcgcac
     8341 agactgggac tggatgatct atcctcaagg tttatatgat caaatcatgc gtgttgttaa
     8401 agattatcct aactatcaca agatttatat cactgaaaat ggtttaggat ataaagatga
     8461 atttattgaa tctgaaaaaa cagttcatga tgatgcacgt attgattatg taagacaaca
     8521 tttgaacgtt attgcagatg ctattaaaga tggtgcaaat gttaaaggtt actttatttg
     8581 gtcattaatg gatgtgttct cttggtcaaa tggttatgaa aaacgatatg gtttattcta
     8641 tgtcgatttt gagactcaag aacgttatcc taagaaaagt gcttattggt acaaagagtt
     8701 agccgaaaca aaagaaatta aataattata gatattaaaa ggcctatcag tcgagtgctg
     8761 gtaggtcttt atttataaaa agagataaat taaacatgca tatacatatt tatatattaa
     8821 tcaatgggtg acacaccatt gaaagctaaa gaagcaatca attaacaaat gttaacggac
     8881 aactttccaa agtcatctta aaagttaatg atgtattcaa aatcaactaa aggacatttt
     8941 taatcacatc ctttatactt atataaaaga catgaatggg agttaaaagt gtgaagaaaa
     9001 tttttgtaat tattacgaca ttgttagcgg tcgctattat tataggctct attattacgg
     9061 tggtgtttag tcaacgacag gcacaaacat ttaaaataca acaacagcaa tttgttaaaa
     9121 agccaattcc aacattgttc ttacatggat ttggtggtag tgccaattct gaaaagttta
     9181 tggtgaagca agcagaaaaa agaggggtaa ctaaagatat catcactgct tacgtttcaa
     9241 aggatggtgc agtgacattt aaagggaaac taagaaaaga tgcagtgaat cctattgtga
     9301 aaattgaact tgaaaataac agacaaggct atttggataa gaatgctgcg tggtttaaaa
     9361 atgtactgac taagttacaa tctgaataca atttcgataa attcaacttt gtcggtcact
     9421 caatggggaa cttaacattt gcacaatata tgatgacata tggtaacgac aagtcactgc
     9481 cacagttaaa caaacaagtg aatattgcag ggacatttaa tggtgtttta aatatgaatg
     9541 aagatgtcaa tgaaattaca gttgataaag atggtaaacc aagtcgaatg aatcaacctt
     9601 atcagcaatt acgcgtgcta aaagatattt ataaaggtaa aggtatcgaa gtgctaaata
     9661 tttatggaga tttgaaagat ggtacacatt cagacggcag agtgtctaat agttcttcga
     9721 aatcattaaa atatttactg ggaaatagtc cgaaaagtta tcgagaatct aaatatgaag
     9781 gtgagcctgc acaacatagt cagcttcatg agaatgaaaa tgttgctaat gagttaatcg
     9841 actttttatg gaaaaaataa agcaactgct agtttttcgg gggcaaaaaa gcttgtaata
     9901 ttagagcttt tcaaaaatta tctattgttt gagagtttcc ataaaatgtc taagtcgtaa
     9961 cattttaaat ttatgattat agcaaatata atgtggttga agttacatga atgaatcagt
    10021 gcttaagtca aaatataaca actaagaatg tatttgcata cagacatcac gaagacacaa
    10081 ttcaaactta acgcttaagt aacaaataag taaaagtttc aaataaagaa aggtgattaa
    10141 tgatgcaaaa taaacaaatt ctttttaaca aaataccaga aggcatgcct caagaggata
    10201 cattcaaaat tgaagagact gacacgccta aacttgagag cgatggcgta cttgtacaaa
    10261 cgctgtatat ttcagtggat ccatatatga gaggacgtat gactaaggca gattcgtatg
    10321 tacagccttt tgaaattggt aaaccgattg ttagccatgt cgttgcaaaa gtaattgatt
    10381 cgacactcgc agattataaa aaaggagatg tcgttgtagg tatgttgcct tggcgaatca
    10441 ttaatcatgt ccaagcggat caaattacta aagttccaac gacagatgta ccacttgatt
    10501 tatatttaag tgttttagga atgccaggac aaacagcata tcatggtctg cttgatattg
    10561 gacaacctaa agccggtgat actgttgtgg tttcagctgc atctggtgct gtaggttcag
    10621 ttgtaggtca aattgctaag attaaaggtt gccgtgtagt aggtattgcg ggtggcgaca
    10681 aaaaagtaaa ttatttaaca gggacactgg gctttgacgc tggtattgat tataagaaag
    10741 aggattttgc agaagcgtta gcacaagcag taccagatgg tattgatgtt tattttgaaa
    10801 atgttggtgg aacagttggc gatgaagtat ttaagcattt aaatcgtttt gcacgtgtac
    10861 cagtatgtgg cgcaatttcg tcatataatc atcctgaagc agatatcgga ccacgtattc
    10921 aaggtacgct tattaagaag caagtaatga tgagaggctt cttggtaagt gaattcgcta
    10981 atgcttttaa agaagctagt gaacagcttg caacatgggt tcaagaaggt aaaattcagt
    11041 cacaagtaac cattgaagat ggttttgaaa atgcgccaca tgcatttaaa aatttattta
    11101 ctggcgataa ctttggtaaa caagttatta aagtaacgga ataataaagt atacgtttta
    11161 acaaactaac tttgttaatt gactaaaatt taataatttt ttatctaaag caaatttgtt
    11221 cagtaacatg gtagaaataa gtctaaaatg taatacgaaa gtcgtctgta attatttata
    11281 tattcaaatg aaaaaggagt catgatgctg cgatataagc acacatggct tctattttgt
    11341 ataataagaa ataagttcat aaatgaggtg aaacgatatg aaaatgatag gatttgaaaa
    11401 gccctttaaa ttggaagaag gaaatttatt taaagtatac gaacaaagaa agccaacgcc
    11461 agaaaatgat gatatcttag tcaaagtaaa tagcattagt gtaaatcctg tagatacaaa
    11521 gcaacgacaa atggaagtca ctcaagcacc tcgtgtactt gggtttgatg ctattggtac
    11581 tgttgaagct atcggaccag acgttacatt atttagtccg ggtgacgttg tattttatgc
    11641 tggatcgcca aatagacaag gatcaaatgc gacatatcaa ttggtgtcag aagctatagt
    11701 tgcaaaagca ccacacaaca taagtgctaa tgaagcggtt agtctaccgc taactggtat
    11761 tacggcatat gaaacgtttt ttgatacatt taaaatttca cataatcctg ctgaaaacga
    11821 agggaagtct gtcttaatca ttaatggtgc cggtggtgta ggcagtatag ccactcaaat
    11881 tgctaaacgg tatggtttaa ctgtcattac aacagcatca cgacaagaaa caacagagtg
    11941 gtgtgaaaag atgggcgcag atatcgtatt gaatcataaa gaggatttag tgcgtcaatt
    12001 taaagaaaaa gaaattccat tagtagatta tatattttgc acctataata cagacttgta
    12061 ttacaatact atgattgaac tgataaaacc attagggcat attacaacta tcgtagcttt
    12121 taatgaagat caagatttaa atgcactaaa attaaaaagt ataacattta cacatgaatt
    12181 tatgtttgcg cgtccaattc atcgaacgcc tgatatgatt aaacaacatg agtatttaga
    12241 agatattacg aaaaatatag aatcagggca ttaccaacca acaacgacac aagtttttga
    12301 aggtttatca cctgaaaact tatatcaagc acatcaattg ttggaaaaac aatcaatgat
    12361 tggaaaatta gtaattaata tatagtttag taagtaaagt cgccttatgc agaaaatact
    12421 gtatagggcg acttattttt tgtgtctagc gactattgat tctaaatgga aatatggcaa
    12481 ctttgcaggt atcaattcaa taatgcatta gaaaggcatt acttaaatac aataaaatac
    12541 tttgttgctc aatgataaat aatttgagtg tgttttatat atcattttta aaagatgagg
    12601 cattattaat tgtgtctata taaaattata aatgattaaa atgttgcagt tatgggtatg
    12661 aacttaccat tacacagaaa gggtttgtat ttatgaaaaa taaatcagta agtctagtgt
    12721 tttgggtatc tttagtgata tgtacgatat ttgttgcttt tggagcaata tttccgaaac
    12781 agttagaaaa actcacgcaa aacattacta catttattgc gctacatttt tcttggtatt
    12841 atttattgct cgttttagtt attttatttg tctgtgtgta tattttattt tcgagatacg
    12901 caaacattac attaggtgaa gaaggcgaag atcctgaatt ctcattgccg tcttggtttg
    12961 ctatgttatt tagtgctggt atgggaatag gacttgtgtt ctggacaacg gcagaaccaa
    13021 tcagtcatgc cttcaaatta acgcctattc ataaagctgg cacgcaatct gcaattaatg
    13081 atgcaatgca gttttcattt ttccattggg ggattcatgc atgggcagtc tatggtattg
    13141 tcgcgcttgt gtttgcatat tttagctttc ataaaggata tcctggctta gtcagcgcta
    13201 cattgacgcc tttattaggt gaaaaggcaa tgcgtggacc tttaggtggt gccattgatg
    13261 tattggcagt catagctaca gtaacaggcg ttgctgcaac attaggtttc ggtgcattgc
    13321 aaataaacga aggtttacat tttttattta atgtaccgag taatttcacg atgcaagtaa
    13381 tcttgattgt cattgcaacc atattattta cttggtcagc atggtcaggt atagataaag
    13441 gtatcaaaac gttgagtaat atcaacatgt tacttgcttt tgtagtactt attggtcttt
    13501 ttattgttgg gccaacgcta tacattttaa atacatttac gaatggtcta ggtaattata
    13561 ttgcaaattt ctttagtatg agtttacgca ttccgtcagg cggtcaaaaa ttccaatggt
    13621 tacaaaattg gacaatcttt tactgggcat ggtggatatc ttgggcacca tttgtaggta
    13681 tatttattgc acgtgtgtca aaaggacgta cgattaaaga atttattcta ggtgtacttt
    13741 ttgtcccggc attagtttgc tttatattct ttgcggtatt cggcgcgtca gcaatttact
    13801 tacaagataa tcatattgcc gatattgcta aagcagcgac agaaacagca acttttgcaa
    13861 cgctacaaca ttacccacta ggttttgtat taagcttaat tactttactt gttattatga
    13921 ttttctttgt tacatcagcg gattcggcaa cttatgtttt aggtatgttg agttcaagag
    13981 gtgatattaa tccgaagtca tttgtcaaag tgagttgggg tatcattatg gcattatttg
    14041 caattattat gatttacaca ggtggtacgc aagcgataca aaatttatta atcattgctg
    14101 cgttaccatt ttccgtggtc attatagcga tgatatggtc gcttttaaaa tctttgagtg
    14161 aggagaaacc gagaaatagt aataaggttt taattaaaca tcgtgaccca gatattttag
    14221 aataccgttc ttcaaaacat attgatgaaa attaattaat aatgaggttt tatttacaaa
    14281 aactagggaa tatattacta aatggtactt catcttatat attaaaaata aatttaaagt
    14341 tattaattta ttatgtttaa gaaaaaaact gatgggtaca tatttaatat gtttttaaat
    14401 taactttaac taaggaggtg tcgttagggg tgaaacgact taaaaacttt atccttggct
    14461 tattaatagt agtaatcgtg gggttcctgt tattcatgta tatccaagat ggacgtataa
    14521 ctgaatatca agactacttc cttcaatttg aatggttcca accattactt atttcacttg
    14581 ctgcactatt gatattaata ggtcttatat tagtatttag tatcttcaaa cctacgcatc
    14641 gaaaacctgg attatacaaa gacttcgatg atggtcatgt ttacgtatct cgtaaagctg
    14701 ttgaaaagac ggcattcgat acggttgcaa aatatgatca agtaagacaa ccaaatgtag
    14761 ttgcgaaact atataacaaa aaaaataaat cttatatcga cattaaaact gactttttcg
    14821 taccaaataa tgttcaagtt caatctttaa ctgaagcaat tcgttcggat attaaaaaga
    14881 atgtagaata tttcacagaa atgccagtac gtaagttaga agttaatgtg agagatcaga
    14941 aaacttctgg tccacgagtg ttgtaaggga ggtatatcat ggctaacaat cataaccaaa
    15001 acggacaaga ctctacacaa caagtgatta attttttaaa agtatttaaa tggagaatcg
    15061 ttggtttcct agcgtttcta ttaatcgcga tattattctt aacgttaggt ttttggaaaa
    15121 cagtacttat catcgtttta tgtttaattg gtgtaggtat tgggtatatg aaagaccgta
    15181 agcaagattt tatgaatttt ttaaatcgat ggagttaatt catcaattta gaatttaatt
    15241 aaaaaaatat aaaaattaac aagcaaaagg agaatctatt atgactgtag ataacaataa
    15301 agcaaaacaa gcatacgaca atcaaactgg tgttaacgaa aaagaaagag aagagcgtca
    15361 aaaacaacaa gaacaaaatc aagagcctca attcaaaaat aaattaacat tctctgatga
    15421 agttgttgaa aaaattgctg gtatcgctgc acgtgaagtt aaaggtatct tagacatgaa
    15481 aggtggctta actgatacat tcactaatgc attctcaagt ggaaataacg ttactcaagg
    15541 tgtatctgtt gaagttggtg aaaaacaagc tgctgtagac ttaaaagtaa ttttagaata
    15601 tggtgaatca gcacctaaaa tcttccgtaa agtaactgaa ttagtaaaag aacaagttaa
    15661 atatattact ggtttagatg ttgttgaagt taacatgcaa gttgacgatg taatgactca
    15721 aaaagaatgg aaacaaaaac acgaaaaaaa taatgaaaac aataaccaag aaagacaagg
    15781 tttacaataa gttattcgat aaaaagctcg gcactcaatt taagtgccga gcttttttgt
    15841 gatttttaat aatttgaata tggaaaatgt attattctct catttgtata gattgtattt
    15901 aataagttaa tgtaatcctt gagctcacga ttaataaaat tctataacct taattatttt
    15961 ctcgatacaa agggttatta actttaatat aagtataatg atgtgcctca tcttcaagac
    16021 gcattgttgt aacacactta taatctataa atggcgcgaa catggtatct ttaatttcat
    16081 ttatgcgatt ctcatttact tgattagatt tgtgtgtcga aagtacaagt tgatcaaaaa
    16141 tgttatctag tacatcacga acgatatacc acatatgtct ttctaagttt gaatcatttg
    16201 atgctttaga gattgtaaga attaattcgc ctaaatggtt ttgaacggta gaataaaagg
    16261 ctttgttaaa cacagacgtt tttgaatcag taagaattct tgatttttca tggaaatgag
    16321 atgtactgta tcccatttca tttagttgta ctttatcaat tcttaaacct tcaaagtctc
    16381 taatatacat cgtgtccaat agaccatctt tccgaaatgt tgcaatagca ttttgcaagt
    16441 gagcctctaa tgcaatgcca tatttagtaa ctagtgggat tacgagacca agcaatgctt
    16501 tactataagt ttcaatccac gatttcgccg atgattcaaa attagacaat gatgcagctg
    16561 attgataacg tttaatcaat gtcacgatag gtgattcatt gttaaatggg taggttgcaa
    16621 ctaagcttga aggaatcatt ggtgtgactt cttgtggaat catttggtat atgttttttc
    16681 taaataatgt acctaattgt tcacttcgtt cagtttgata gtctacttcg tcttgctcat
    16741 tataaaaatg aataccagta acttcatcaa taattgttga tgcataggac ttaaatatga
    16801 catctttctc caaaatatca tttaaaatac gtgtcattag tggaccattg tgcgtcgttt
    16861 gttctgataa tgtacgaatt tcacctgtaa tatgaacgtt tgtcgacaat ttgatgtgtg
    16921 gcgacatagc tgggtattta ggaactaatg ttctgaaaga taaaccggca taataatcca
    16981 acgtatgttt tgcttcaatg attaattctt tatctacttc tgcttgataa tcagaatgta
    17041 atacgtcatc taattgccat ggatgaacaa tcataatgtg ataatcatta aagttaaact
    17101 ttggcgtaaa ttcattttct aattgtttaa ttaattccgg aaatagttga tgaacagttg
    17161 tatcataatc tttagacagt gacatagtcc ggcctaattt actgtgaatc agtactattt
    17221 tcaacttaat aggttgattg aattctgaag aatataggaa tgtttgtaat gcatttaaac
    17281 ctttacgtaa tttagcccca ggatgtagcg gatgaccttc aataacggct tgctctgaac
    17341 gcaagtaact atcttcgcta ttttcgataa tattaaataa aggtgcagaa tcatgttgca
    17401 ttgacagtgc ttgatagcta attgcaaatg tcatattagt tgcactgttt attaaatctt
    17461 gctgaaattg atcactagca gcatttttta aatctggtgc ttcaattaaa atacactcaa
    17521 gaatttcatt tggatggtgt actcgtgtaa tcgtatttgt aatgtcatct ttaatgtaga
    17581 aagggccttc aacatcaatt cgatcaaagg cgtgttctcc agtgatagga gcatataatg
    17641 tttgcttagc ttgtgggaac tggatttcta gtatatgagt cgttgagata tctaacataa
    17701 tcaaatcacg actcaatatt ttcttacttt gagtgcgcgc tttaactaag ttttcgcgat
    17761 gcattgatgt gactaatctc tgagtgactt tatctcttcc ttgtaaaatc atctctttaa
    17821 aaatattagc ccaatcgcta ttatgttgtt gtaaaaataa atatgtttct tgttcttctt
    17881 tgttaaattt taatgtctgt tctttaaaaa ttaagttcaa gttcataatt cacctctatg
    17941 aaatatttta caaaagcaag atagatttgt ataatccata ttaatgataa tgattcttat
    18001 tatcaacaga atgcgggtgt aagttttatg acaaaatatt tttttagcag ttcttttcta
    18061 ctatttctag gtaattggat tggacaaata gggctaaatt ggtttgtact taccacttat
    18121 cataacgcag tttatctggg gattgtcaat ttttgcagac ttgtaccaat attattacta
    18181 agtgtgtggg caggggcaat tgccgataaa tatgataaag ggcgattgct gagaattaca
    18241 atttcatcat catttttagt aactgcaatt ttatgtgtgc tcacgtatag tttcactgca
    18301 attccaatta gcgtcattat tatatatgcg acattaagag gaattttaag tgctgttgaa
    18361 acacctttaa gacaagcaat cttaccagat ttatcagata aaatatctac tacacaagct
    18421 gtatcatttc attcattcat cattaatatt tgtcgttcaa tagggcctgc cattgctggt
    18481 gtcatattag cagtctatca tgcgccaaca acatttcttg cacaggcaat ttgttatttt
    18541 atcgcagttt tattatgttt accattacat tttaaagtaa ctaaaatacc tgaagatgca
    18601 acaagataca tgccgttaaa agttattata gattacttca aattacatat ggaaggtcga
    18661 caaatattta taacatcatt attgattatg gcgacaggtt tttcatatac gacactttta
    18721 ccagttttga caaacaaagt atttccgggg aaatctgaaa tatttggtat cgctatgacg
    18781 atgtgtgcca ttggtggtat tattgcaacg ctagttttac ctagagtact taaatatatt
    18841 ggtatggtaa atatgtatta tttaagttca cttttatttg gcattgcttt gttaggtgtg
    18901 gtatttcaca atattgtcat catgttcatt tgtattacat tgattgggtt atttagtcaa
    18961 tgggcacgta cgacaaatcg cgtttatttt caaaataatg ttaaagatta tgaacgtggt
    19021 aaagtactga gtattattat gatggataga ggtatgattc cattgggaag tctgttaatg
    19081 agtatatgtg cagatgtgtt tggcattgtt agaacttttt caataatggg aataagtact
    19141 atatgcatta caatggtatt ctattttata aatagaaagt tgaagttaaa gttggaggaa
    19201 agtaatcatg gtatatcttg aatgggcaaa ggcagataga aatattcaat atcgtgtaat
    19261 taacgccatt attaaagaac gtatttaccc cgagcaaaca tttatttcgc aaaaaggatc
    19321 tttaattgaa attcagtatc atatgcatgt gttgactatt gaaattgtta gaaaaagtgc
    19381 attagaacgc tacgagttta caggtgatat tacttattta aataaaggtg aaacgtcatt
    19441 aattataact ttagaaggtt tattagatgt gttgaatcat gactttgata tccctatttc
    19501 agagcgacta cgcgaagagt taatacacag tcgagatagt ttagttgaaa catataagca
    19561 aatgtctcac agacaaacgt taataagtca aagttttaaa ttttcaagat taccacaaga
    19621 tattaacttt ttttcatggt tacaacatgt aaaagatagt gataagacag atgatttaac
    19681 ttattctgag agtttggtac cagaggggca tccaacacac cctttaacca aaacgaaatt
    19741 gcccttaact atggaagaag tacgagcata tgcacctgag tttgaaaaag aaatcccttt
    19801 gcaaattatg atgattgaaa aagaccatgt tgtgtgcaca gctatggatg gtaatgatca
    19861 atttattatt gatgaaataa ttcctgaata ctacaatcag attcgtgtgt ttttgaagag
    19921 tttaggtttg aaaagtgaag actatagagc gattttagta catccttggc aatatgatca
    19981 tacgataggg aaatattttg aagcatggat tgctaaaaaa atattaattc caacgccgtt
    20041 tacaatactt tcaaaagcaa ctttatcatt caggacgatg tctttaattg ataaaccata
    20101 tcatgttaag ttgcccgtcg atgcacaagc aacaagtgcc gttagaacag tctcaactgt
    20161 gactactgta gatggaccaa agttaagtta tgctttacaa aacatgttga atcaatatcc
    20221 aggatttaaa gttgctatgg aaccgttcgg tgaatatgca aatgttgata aagatagggc
    20281 acgtcagctt gcttgtatca taagacaaaa gcctgaaatt gatggaaaag gtgcaacagt
    20341 agttagtgca agtctagtta ataaaaatcc aatagatcaa aaagttatcg tggatagtta
    20401 cttagagtgg ttaaatcaag gaattactaa agaaagtatt acgacattta ttgaacgata
    20461 ctctcaagca ttaatcccgc ctttaattgc ttttattcaa aattatggaa ttgcattaga
    20521 agcacacatg caaaatacag tagtgaactt ggggccacat tttgacattc aatttttagt
    20581 gagagattta ggtggttcta gaattgattt agaaacatta caacatcgtg tatcagatat
    20641 taaaattaca aatgatagtt taatagctga ttctatagat gcagtgattg caaaattcca
    20701 acatgctgtt attcaaaatc aaatggcaga attaatccat cattttaatc agtatgattg
    20761 tgttgaagaa gccgaattat ttaacatagt acagcaagta gtagcgcatg ccattaaccc
    20821 aacactacca catgcaaatg agttaaaaga tattttgttt ggaccaacaa ttactgtcaa
    20881 agcgttgtta aatatgagaa tggaaaataa agtaaagcaa tatttaaata ttgagttaga
    20941 taatccgata aaaaaagagg tgtagtacta catggcacac gttaacataa atatatcgaa
    21001 gattaaatat aacgccaaag tacttcaaac agtttttcaa agtaaaaata tgcaattcac
    21061 accagtaatt aagtgcatag ctggtgaccg tacaattgta gaaagcttaa aagcgttagg
    21121 tatcaatcat gttgcagaat ccagattgga taacataatt agtattgcag atcaggattt
    21181 aacatatacg ttattgcgta cgcctgccaa aaaagagatt tcagatatga tagaaaaagt
    21241 tgatatgagt atccaaacag aattatctac catacatcaa ataaatgaag tagcagaagt
    21301 tttaggaaaa aaacataaga ttttattaat ggtagattgg aaagatggcc gtgaaggtgt
    21361 tctgacttat gatgttttag attatattaa agagattatt cacttgaaaa atatccattt
    21421 tgtaggttta gcattcaatt ttatgtgttt taaatctgat gcaccaagtg atgatgatat
    21481 ctttatgatt aatcgattcg tctcagctgt tgaaagagaa ataggatatc gattgaaaat
    21541 tatttcaggt ggtaattcga gcatgttacc acaactttta tataatgatt tgggtaaaat
    21601 aaatgaattg cgaataggtg aaacgttgtt tagaggtgtt gatacaacga caaatcaagc
    21661 gatagctatg ttatatcaag atgcgattac attggaggct gagattttag aaattaaacc
    21721 tcgagttaat acacaaacgc atgaatcatt cttacaagct attgtagata ttggttattt
    21781 agatacaaaa gtcgataata tttcacctat ggatcagcat atcaatattt taggcgcttc
    21841 gagtgatcat ttaatgcttg atttaaatgg tcaaggacac tatcaagttg gagatcatat
    21901 atctttttca ttaaattatg aagcactttc tcattcgatg tatatgaaaa atttacataa
    21961 ggtatatatt gatgattcaa agattgatac gctacttcaa aattttgatg tgaaatctcc
    22021 tgctatggta aaccaatatt aatatattta tcaataagtc taagttgaca agttatacta
    22081 catatcgttt gcacggttgt atcataattg ttcaacttag attttttgta tttgttgatt
    22141 tatcaaatta agtgcaacag ttcgtcacat aaaattgcaa cagataatat cagctgaatt
    22201 acagggataa cggtcatact aaatggtgtc aattgtatta atgcaaagat gatatagcaa
    22261 tgatacatta tcaatatttt ttctaaggaa atgtgctaat tgtagtcata attactagag
    22321 gaaatatata gtcatacatt ttagtaatat aaaaaagatt gaacgctact tgacaatgat
    22381 aattgttatc aataaaataa taaatgaagt tatacatatt aaggagtgga acgatgagag
    22441 gtctaaaaac ttttagtata ttgggattaa tagttgcctt acttttagtt gcagcttgtg
    22501 gtaatacgga taattcaagt aaaaaagaat catcaactaa agatactatt tcggtaaaag
    22561 atgaaaatgg tacagtaaaa gtacctaaag atgcaaaacg tatcgttgta ttagagtact
    22621 catttgcaga tgcattagca gcattagacg ttaatccagt tggtattgct gatgatggta
    22681 agaaaaaacg tatcattaaa ccagttagag aaaaaattgg ggattatact tctgtaggta
    22741 cacgtaaaca gccaaactta gaagaaatta gtaaattaaa accggattta attatcgctg
    22801 atagcagtag acataaaggt attaataaag aattaaacaa aattgcacca acattatcat
    22861 taaagagttt tgatggagac tacaaacaaa acattaattc gttcaaaaca attgctaaag
    22921 ctttaaataa agaaaaagaa ggcgaaaaac gtcttgctga gcatgataaa ttaatcaata
    22981 agtataaaga tgaaattaaa tttgatagaa atcaaaaagt gcttccagca gtagttgcta
    23041 aagctggttt attagcacat ccaaactatt catatgttgg acaattttta aacgaactag
    23101 gatttaaaaa tgcattaagt gacgatgtaa caaaaggttt aagtaaatat ttgaaaggac
    23161 cttacttaca attagacact gaacatttag ctgatttaaa tccagagcgt atgatcatta
    23221 tgactgatca cgctaaaaaa gattctgctg aattcaagaa attacaagaa gatgcaacat
    23281 ggaaaaaatt gaatgcagtt aaaaataatc gcgtggatat tgttgaccgt gatgtttggg
    23341 caagatctcg tggcttaatt tcttctgaag aaatggctaa agaacttgtt gaattatcaa
    23401 aaaaagaaca aaagtaaggt ggaagtaaat ggctataaaa gaaataagta gccaatctgc
    23461 catagatcat aaaagaaaaa gacgcacaac actcacgtat atagtgagtt tgtgctttct
    23521 ttttatttgt atatatttaa atatggcgat tggttcttcg aaaattaatt ttagcgatat
    23581 cgttcactat gttactggtc atacagatac gaaagcaacg tttttattgc ataatgtacg
    23641 tatgccaagg atgattgcag ggttatttat tggcggtgca ttagcggtat ctggtttgtt
    23701 aatgcaagca atgacacgca atccgctagc atcacctaaa atttttggtg tcagttctgg
    23761 tgcatctttt ttcattgtat ttgtgacgat tatcattcca tcattagaat attatgcatt
    23821 atatttaggt gtgataggtg catttatagg tggtttaact gtttatacac tttcaggtgc
    23881 tacgaaaggt atgacaccca ttaaattagc attggctggt atggccatcc atttgttttt
    23941 tagtagtatg accgaaggta ttattatttt gaatgagaat tcaaacgaac aagtgatgtt
    24001 ttggcttgtc ggttcattat ccagtatgaa gtgggatgaa attttaacca ttctaccatg
    24061 gcttattggt gctttaattg taacgatttt tattggacgt caactaacga taatggaact
    24121 aggcgatgac attgctaagg gattaggtca aaatataaat aaagttagaa ttatcattgg
    24181 actattagtc attattctta caggtatgtc ggtatcagtg gcaggaccaa ttggctttgt
    24241 tggcttaatc gtaccgcata tagtcaaacg ctatgtgagt aagaattatt tagtcatgat
    24301 tccattaaca tttattatag gtgcagattt gttactacta tcagatgtat taagtcgttt
    24361 aattacatat ccttatgaat caccagtagg gattgttaca tcattcgtag gtgcacttta
    24421 cttcttattt ataacgatta aaggggtgaa aagaatatga tgacaacgaa aattatacgt
    24481 cgttatagtt taatcacaat ccttttaatt gtgagtatat tcataagttt atgtgtaggg
    24541 tctgtcatga ttcatccgat tgatgctatt aagggaatct ttacacaaga tgattttatt
    24601 ttaaatgagt atcgtatacc tagaacatta cttggtatta ttatcggcag tagcttggct
    24661 atttcaggtg caattataca agctgtaata agaaacccgt tagcctcacc tgatgttata
    24721 ggtatttcta aaggtgccag tttagctgct gtgattatta ttatgacctt tccaacagca
    24781 ccattgtttg tattacctat aggttcattc ttaggtgcct ttgcagtaag tttattttta
    24841 tcatttttaa tttcaaaata tgatgttaaa ggttcaaagc ttgccttaat tggtttagca
    24901 ataggcgcaa tatgtacggc gattgtgcag ttcttattaa tcagaaatcc actggaagca
    24961 aacaaagcgc ttgtttggtt gacaggtagt ctatatggac atgatatgtc gaatatttat
    25021 actatattac cttggttttt aataacgata ccaatcatca ttttattgag ttatcaatta
    25081 gatgtattga atctaggtga tgctgttgca accgcactcg gtttaaaagt taaaactatt
    25141 aaaatgctat tgttaatttt agcagtaata ttagcaggat ccgcaatttc agttgtcggt
    25201 ggtattagct ttttaggatt aattgcacct catattgcga gacaattaat aggaaataaa
    25261 acattacacg ttattatcat gtcggggtta attggtgcta tattgttaac atttggagat
    25321 ggtttagccc gtggcattca tccgccactg gatattcctg ttggggttat cattgcaatt
    25381 atcggtgcac catacttctt aatattatta cgaagaatga aataactaac atatgattag
    25441 agtttaaaaa aggttaagta cgaaatcagt aattgtactt aacctttttt gaattcatct
    25501 tgaaaaatta caagaaatag cgtatattta ataactgtaa ttattcatta ttttgcatac
    25561 ataaacattt tgaaagatag aatgataagg aatatgtttg ttaactgaat agaattctgt
    25621 atatacttaa aacgaataca tagagaaatg gattgatttt atggatagct tttcaaggaa
    25681 agagatagtt attgggagac ttaagtttat tacgatgtct ttaattggta tcttattatt
    25741 tttagtgcca attccagtcg aacaagatgg acagaagcaa acaacgttgc ccgtggcatt
    25801 tttagcaggc gtgcttaaag atgtattagg tggcgtaatg ccatttttaa ttgtgacgat
    25861 aattacttta tcgggtatca taactctgat ttgttcaact attttaaaag ataaattgaa
    25921 acctgacggg ctaatgaaca atgcttttaa cgttagaatt ggttggttaa ttttacgaat
    25981 attagcagtt gtttttgcat ggatgacatt tttacgaatt ggttcaaagg ttatttactc
    26041 agatgaaacg ggtggattat tgttctcaag tttattgcca acattagtgg cagtattctt
    26101 atttgcagct ctatttttac cgctattaat ggaatacggt ttgttagaga tgttgggtcc
    26161 tatttttaga ccggttatga gacctttatt tacattgcca ggacgttcga cggtagataa
    26221 tttagcatca tttattggag acggaactgt tggggtctta attacaagta gacaatatgg
    26281 tgaaggatat tattctcgtc gtgaggcaac agttatttca acgacattta gtgtagtttc
    26341 tatcaccttt gcaattgtag ttgctgaaac agtgcacatg caaaatcaat tttttgcttt
    26401 ctatttatca gtgatagtat cctgcctcgt tgcagcagtc attatgccaa gaatttggcc
    26461 tttaaataaa attcctgatg agtatgctaa ggaggtacct gaaagtgcgc gtactgaggc
    26521 attgccagaa ggaaagacgg cattgagaca tggctttgat actgcaacag aagttggtat
    26581 taaagcaccg ggcgttattg acttttttaa gtcaggatta aaaacagtca ttgatatgtg
    26641 gtttgttatt ttgcctgtag tgatgagtat tggtactatt gctaccatta ttgcgaacta
    26701 cacgcccttt tttgtcatat tgggtaaacc ttttgttcca tttttagagt taatgcaaat
    26761 acctgaagcg gcacaggctt ctcaaacgat tattatcggt tttgccgata tgtttttacc
    26821 ttctatatta attgaaggtg tacaaaatga tataactcgt tttgttattg gggcacttag
    26881 tatatcgcag ttaatatact tgtctgaagt aggtggcgtc atacttggtt cgaagattcc
    26941 tgtaagtatc ggcaaattgt ttatgatttt cttgattaga acaatcatta cactaccaat
    27001 tatttctttg atggcacatc ttttgttata aatatatgtg aaaaaggaca actgttaaaa
    27061 ttatgagaac agttgtcctt ttgtatttgt ttttgctggg tgttgaatca attaaaaaaa
    27121 tcattcatga agccctcacc atgaatgatt tttttataca atttatttag ttaattcatt
    27181 gtaaactttt tgatcatttt gagtataaat aatgtattcg tggtcttcag tatcgataat
    27241 aacgcgattc gttttaccaa acgttgagcc gattctagat acatctttgc tgtctaattt
    27301 aggaactgca tgaatatctt ggtcttgtgt aatattttta atttcacttg taggaatttt
    27361 gatatcagca actctccatt gaatacgcac ttcattatta tctttcttta ctgtcattgc
    27421 cattattcaa acacatcctt taaatatttt gtaaatacat cataaacgat tttgtaatcg
    27481 ttttcaagag ttatgtgata cttttttaaa ttgtgtcata ctaatgaatt ttaacccgaa
    27541 aatatgttcg tgtttttgtt agggatgtgg taaaatgtga gtattaaagg agaacaaata
    27601 tgacaggtaa aacacatgct tcatgcggca tgctcgttgg agcgctaaca acacaatatt
    27661 ttcaaacgga tattttttcg tcggttacgg ttatcattct agcaactcta gctagcttat
    27721 tacccgatat atgtcacact caaagtaaga ttggcagaag gtttaaggta attagttttt
    27781 ttgtgagatt gatctttgga catcgaacct ttacgcattc aatattattt atcgcaatca
    27841 tagcattttt attgcaaatt attcagacgc ctaattatta tatggcagca attataattg
    27901 ggttagtgtc acatgttata ctagatatga taacaccaag aggcgtgaaa ttattttatc
    27961 cgttaccgtt taatgtgaag acaccgattc aatttaaaac aggtggactt gtagacttat
    28021 ctttagctac agcgttaatg gtcggaacag tttatgtttt atttcagcct ttcatcaatg
    28081 atataataag caactggaat actaagtttt tatagaaaat aaattattaa aggttacaaa
    28141 ctcagcagga ggcataaaat gctagataaa aatcaattag ctaaatataa acaagatcat
    28201 ttgtgtgaat atgaaaaaat aatgagtaac aatgaaaaag aagcacttga agagaaggta
    28261 gcgtcattag atttagattt tattgccaaa ttatataacg atttatacat caataagaaa
    28321 acaatagatg atgtatctgc agtttctgaa gtgaaatatg atattaaatc acaaatgagt
    28381 gatgatgaga ttaagcgttt agaggaacaa ggacttcaag ctattaaaga aggacaattt
    28441 gcagtacttt tgatggcagg tggtcaagga acaagacttg gttacaaggg ccctaaaggt
    28501 tcgtttgaaa ttgaaggtgt aagtttattt gaacttcaag ctaaccaatt aaaaacattg
    28561 aatcatcaat ctggacatac gattcaatgg tatattatga caagtgatat caatcatgaa
    28621 gaaactttag cttatttcga agcacatagt tattttggat atgatcaaga agcaatccat
    28681 ttctttaaac aagataatat tgtggcgctt agtgaagaag ggaagttaat tttaaatcaa
    28741 cagggtcgta ttatggaaac gccgaatggt aatggtggcg tgtttaaatc tttagacaag
    28801 gcaggatact tagaagaaat gtctaataat ggcgttaagt atattttctt gaataatatc
    28861 gacaatgttt tagtaaaagt gttggatcca ctatttgcag gatttacggt agaacatgat
    28921 tacgatatta cttctaaaac gattcaacca aaaccaggag aaagtgttgg acgtttagtt
    28981 aatgtcgatt gtaaagatac agtactggag tattcagaat tagatccaga agttgcaaat
    29041 caatttaaca atgctaatat cggtattcat gcatttaaac ttggttttat tttaaacgct
    29101 gtaaatcgtg agttacctta tcatttagct attaagaatt tgaaacaatt agatgagaac
    29161 tttggggtta ttgaacaacc aactttaaaa ttcgaattgt tctattttga catatttact
    29221 tatggaacta gttttgtcac gttacaagtt cctagagaag aagaattttc acctcttaaa
    29281 aataaagaag gtaaagatag tgttgcaact gcaacagaag atttacgtag aatgggttta
    29341 atttaatgat aggcggtgac tatgatgtcc aaatcaacta aaaaaagtaa tgacacaatc
    29401 gtagaaacat ttaaggatat catcccttta tcatttggag aagaaattgg taatgcggca
    29461 tctcatggtg ttgcagcttt gttaacatta ttagtgttac cctatgcggc tgtgcacagt
    29521 tatattgcac atggtacgct tgcgtctgtg agtatgtcta tttatgttat ttctatcttt
    29581 atgatgttta tttcatctac tatttatcat tctatgcaaa atgaaacacc tcataaatat
    29641 attttaagga ttatcgacca cagtatgatt tacgtagcaa tttctggtac atatacgcct
    29701 attttgttaa cagttgtagg tggttggata ggttggacag tatttatttt attatgggga
    29761 acaacacttt ggggtatttt atataaatcc atagctgtca aagttaacca aaagttaagc
    29821 ttaattgtct atttagtgat gggctgggta ggtattatct tgttaccaat tatttttatt
    29881 cgcacatcat ggatattcat attattcatt ttcttaggtg gtgtgtctta tacaattggt
    29941 gcatggtttt atgcacaaaa gaatagacca tattttcata tgatttggca tatttttatt
    30001 gtaatagcat cactattgca tttaattgca atcttatatt ttatgtagtc gtttagaaaa
    30061 gctgggggaa actccgcttt ttttattaca taaatcattc taatagggga ttgtattgta
    30121 atagaattat c
//
LOCUS       DAMZLE010000016        29308 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMZLE010000016 DAMZLE010000000
VERSION     DAMZLE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.016"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            854..2191
                     /locus_tag="POB92_000713"
     CDS             854..2191
                     /locus_tag="POB92_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDI6578233.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQVLAVLPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            2193..2810
                     /locus_tag="POB92_000714"
     CDS             2193..2810
                     /locus_tag="POB92_000714"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI6578234.1"
                     /translation="MSAFITFEGPEGSGKTTVINKVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2838..3167
                     /locus_tag="POB92_000715"
     CDS             2838..3167
                     /locus_tag="POB92_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDI6578235.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            3381..4307
                     /locus_tag="POB92_000716"
     CDS             3381..4307
                     /locus_tag="POB92_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI6578236.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            4308..5111
                     /locus_tag="POB92_000717"
     CDS             4308..5111
                     /locus_tag="POB92_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDI6578237.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRVADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            5128..5475
                     /gene="yabA"
                     /locus_tag="POB92_000718"
     CDS             5128..5475
                     /gene="yabA"
                     /locus_tag="POB92_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDI6578238.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5749..6474
                     /locus_tag="POB92_000719"
     CDS             5749..6474
                     /locus_tag="POB92_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDI6578239.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYITMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6467..6715
                     /locus_tag="POB92_000720"
     CDS             6467..6715
                     /locus_tag="POB92_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI6578240.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6717..7556
                     /gene="rsmI"
                     /locus_tag="POB92_000721"
     CDS             6717..7556
                     /gene="rsmI"
                     /locus_tag="POB92_000721"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI6578241.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7841..9814
                     /gene="metG"
                     /locus_tag="POB92_000722"
     CDS             7841..9814
                     /gene="metG"
                     /locus_tag="POB92_000722"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI6578242.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIINAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9845..10618
                     /locus_tag="POB92_000723"
     CDS             9845..10618
                     /locus_tag="POB92_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDI6578243.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10785..11321
                     /gene="rnmV"
                     /locus_tag="POB92_000724"
     CDS             10785..11321
                     /gene="rnmV"
                     /locus_tag="POB92_000724"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDI6578244.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            11332..12225
                     /gene="rsmA"
                     /locus_tag="POB92_000725"
     CDS             11332..12225
                     /gene="rsmA"
                     /locus_tag="POB92_000725"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDI6578245.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            12325..12588
                     /locus_tag="POB92_000726"
     CDS             12325..12588
                     /locus_tag="POB92_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDI6578246.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12898..13746
                     /locus_tag="POB92_000727"
     CDS             12898..13746
                     /locus_tag="POB92_000727"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI6578247.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13760..14584
                     /gene="purR"
                     /locus_tag="POB92_000728"
     CDS             13760..14584
                     /gene="purR"
                     /locus_tag="POB92_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDI6578248.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGISVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14601..14981
                     /locus_tag="POB92_000729"
     CDS             14601..14981
                     /locus_tag="POB92_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6578249.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGEIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            15053..15355
                     /gene="spoVG"
                     /locus_tag="POB92_000730"
     CDS             15053..15355
                     /gene="spoVG"
                     /locus_tag="POB92_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDI6578250.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15545..15676
                     /locus_tag="POB92_000731"
     CDS             15545..15676
                     /locus_tag="POB92_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578251.1"
                     /translation="MCLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            15699..17051
                     /gene="glmU"
                     /locus_tag="POB92_000732"
     CDS             15699..17051
                     /gene="glmU"
                     /locus_tag="POB92_000732"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI6578252.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            17198..18163
                     /locus_tag="POB92_000733"
     CDS             17198..18163
                     /locus_tag="POB92_000733"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI6578253.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            18313..18966
                     /locus_tag="POB92_000734"
     CDS             18313..18966
                     /locus_tag="POB92_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDI6578254.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            19277..19849
                     /locus_tag="POB92_000735"
     CDS             19277..19849
                     /locus_tag="POB92_000735"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6578255.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19849..23355
                     /gene="mfd"
                     /locus_tag="POB92_000736"
     CDS             19849..23355
                     /gene="mfd"
                     /locus_tag="POB92_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI6578256.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTKIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            23345..24871
                     /locus_tag="POB92_000737"
     CDS             23345..24871
                     /locus_tag="POB92_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI6578257.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24871..26064
                     /locus_tag="POB92_000738"
     CDS             24871..26064
                     /locus_tag="POB92_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI6578258.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEK
                     GESNT"
     gene            26061..26324
                     /locus_tag="POB92_000739"
     CDS             26061..26324
                     /locus_tag="POB92_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDI6578259.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            26342..26734
                     /locus_tag="POB92_000740"
     CDS             26342..26734
                     /locus_tag="POB92_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDI6578260.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26839..27240
                     /locus_tag="POB92_000741"
     CDS             26839..27240
                     /locus_tag="POB92_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDI6578261.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            27420..28715
                     /gene="tilS"
                     /locus_tag="POB92_000742"
     CDS             27420..28715
                     /gene="tilS"
                     /locus_tag="POB92_000742"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI6578262.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEVYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKASHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            28720..29259
                     /gene="hpt"
                     /locus_tag="POB92_000743"
     CDS             28720..29259
                     /gene="hpt"
                     /locus_tag="POB92_000743"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI6578263.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
BASE COUNT        10624 a         3939 c         5871 g         8874 t
ORIGIN      
        1 atgtctaaaa catcaaaata aaaagcaaac acaaagaaaa atggcttggc gaagtgaaaa
       61 cgtttgaatc tgacgaaacg agaaaagagc gcaacgagtt tagtagagct aaatgagtaa
      121 gcgagagccg aaggagagga aagaagcaag cgattgtcac aagtcaagaa aggttcttag
      181 cgacgatggt atccaactta cgttccgcta gagtagaacg ttgccaggca aaaatggatg
      241 cgatgagccg cattgagacc gtgaggtctc tttttttatg tctaaaacat caaaaaaaaa
      301 agcaaacaca aagaaaaatg gcttggcgaa gtgaaaacgt ttgaatctga cgaaacgaga
      361 aaagagcgca acgagtttag tagagctaaa tgagtaagtg agagccgaaa aagaggaaag
      421 aagcaagcaa ttgtcacaag tcaagaaagg tctttagcga cgatggtagc caacttacgt
      481 tccgctagag tagaacgttg ctaggcaaga aatggatgcg atgagccgca ttgagttttg
      541 gaaatgatat tttaataatg tgcacttttg attgtttaaa tctgtgacta gaagtataag
      601 tgaagacatt cagaagtatt ataaaaagtg aacagcagta agatagtttt taatcataaa
      661 tcatcttact gctgttttta ggttttatgt ctaatatttt ttaaatcgaa atacaaaaag
      721 gaaattaatt attatacaat agacaagcta ttgcataagt aacactaact tttgtcaaag
      781 aagtgttact atataattaa tacttttgaa agtaactaat tccaaaacag tgtaaataaa
      841 ggaagcgtat atcatgaagc aacctatttt aaataaatta gaaagtttaa atcaagaaga
      901 agcgatttct ttgcatgttc cgggtcataa aaatatgact atcggtcatt tatctcaatt
      961 atcaatgaca atggataaaa ctgaaatacc tggattagat gatttacatc atcctgaaga
     1021 agtcattttg gaaagtatga agcaggtgga gaaacattca gattatgatg cttatttctt
     1081 agtgaatggc accacttcag gaatattatc tgtcatccag tctttttcac agaaaaaagg
     1141 cgatatctta atggcaagaa atgtacataa atctgtatta catgcgctcg atattagcca
     1201 acaagaaggg cattttattg aaacgcatca aagtccgtta acgaatcatt ataataaagt
     1261 taatttaagc cgtttgaata atgacggtca caaacttgct gtgttgactt atcctaacta
     1321 ttacggtgaa acatttaatg tagaagaggt tatcaaatct ttgcaccaat taaatattcc
     1381 tgtactcatt gacgaagcac acggcgcgca ctttggattg caaggatttc cagattctac
     1441 attaaattat caagctgact atgttgttca atcttttcat aaaacgttac cagctttaac
     1501 gatgggctcg gtactttata ttcataaaaa tgcaccttat agagaaacta ttatagaata
     1561 tctaagctac ttccaaacat ctagtccttc gtatttgatt atggctagtt tagagtcagc
     1621 tgccgagttc tataaaacat atgatagtac cgtgtttttt gataagagag cgcaattaat
     1681 cgaatgtttg gagaagaagg gttttgaaat gcttcaagtt gatgatccgt tgaagttgct
     1741 gataaaatat gaaggtttta caggtcatga tattcaaaat tggtttatga atgcacatat
     1801 ctatttagaa ttagcggacg actatcaagt attagcggta ttgccgttat ggcatcatga
     1861 tgatacgtat ttatttgatt cgcttttacg taaaattgaa gatatgattt taccgaaaaa
     1921 atcagtttct aaagttaaac aaacacaact tttaacaact gaaggtaact ataaaccaaa
     1981 acgctttgaa tatgttactt ggtgtgattt gaaaaaggca aaaggtaaag ttctggcgcg
     2041 acatattgtc ccgtatccgc cagggattcc tattattttc aaaggagaaa caataactga
     2101 aaatatgata gaattagtaa atgaatatct ggaaactgga atgatagttg aaggaattaa
     2161 aaataataaa attttagttg aggatgaata aaatgtcagc ttttataact tttgagggcc
     2221 cagaaggctc tggaaaaaca actgtaatta ataaagttta ccatagatta gtaaaagatt
     2281 atgatgtcat tatgacaaga gaaccgggcg gcgttcctac tggtgaagaa atacgtaaaa
     2341 ttgtattaga aggcaatgat atggacatta gaactgaagc aatgttattt gctgcatcta
     2401 gaagagaaca tcttgtatta aaggtcatac cagctttaaa agaaggtaag gttgtgttgt
     2461 gtgatcgcta tatcgatagt tcattagctt atcaaggtta tgctagaggg attggcgttg
     2521 aagaagtaag agcattaaac gaatttgcaa taaatggatt atatccagac ttgacgattt
     2581 atttgaatgt tagtgctgaa gtaggccgcg aacgtattat taaaaattca agagatcaaa
     2641 atagattaga tcaagaagat ttaaagtttc acgaaaaagt aattgaaggt taccaagaaa
     2701 tcattcataa tgaatcacaa cggttcaaaa gcgttaatgc agatcaacct cttgaaaatg
     2761 ttgttgaaga cacgtatcaa actatcatca aatatttaga aaagatatga tataattgtt
     2821 agaagaggtg ttataaaatg aaaatgatta tagcgatcgt acaagatcaa gatagtcagg
     2881 aacttgcaga tcaacttgtt aaaaataact ttagagcaac aaaattggca acaacaggtg
     2941 ggtttttaag agcaggtaat acaacattct tatgtggtgt caatgatgac cgtgtagatg
     3001 aaatattgtc tgtgattaat caaacgtgtg gtaatagaga acagttggtt tcacctatta
     3061 cacctatggg aggcagtgcg gattcgtaca ttccatatcc agttgaagtt gaagttggcg
     3121 gtgctactgt atttgttatg ccagttgatg cattccatca attttaattc tataatacaa
     3181 tcatcaatta gagatactta aaatagtgta ttaataagtt attaacaatt ttgggttgct
     3241 tgactgcgac tagttcagat gccaatagat ttgatttttg tggttctaaa aataatcaca
     3301 aatcatggtc gctattgttg cagtaattag ttgctctttg gcaacctttt tatataaaag
     3361 caaaagggag tttgtaatga atggatgaac agcaacaatt gacgaatgca tatcattcaa
     3421 ataaattatc gcatgcctat ttatttgaag gtgatgatgc acaaacgatg aaacaagttg
     3481 cgattaattt tgcaaagctt attttatgtc aaacagatag tcaatgtgaa acaaaggtta
     3541 gtacatataa tcatccagac tttatgtata tatcaacaac tgagaatgca attaagaaag
     3601 aacaagttga acaacttgtg cgtcatatga atcaacttcc tatagaaagc acaaataaag
     3661 tgtacatcat tgaagacttt gaaaagttaa ctgttcaagg ggaaaacagt atcttgaaat
     3721 ttcttgaaga accaccggac aatacgattg ctattttatt gtctacaaaa cctgagcaaa
     3781 ttttagacac aatccattca aggtgtcagc atgtatattt caagcctatt gataaagaaa
     3841 agtttataaa tagattagtt gaacaagaca tgtctaagcc agtagctgaa atgattagta
     3901 cttatactac gcaaatagat aatgcaatgg ctttaaatga agaatttgat ttattagcat
     3961 taaggaaatc agttatacgt tggtgtgaat tgttgcttac taataagcca atggcactta
     4021 taggtattat tgatttattg aaacaggcta aaaataaaaa actgcaatct ttaactattg
     4081 cagctgtgaa tggtttcttc gaagatatca tacatacaaa ggtaaatgta gaggataaac
     4141 aaatatatag tgatttaaaa aatgatattg atcaatatgc gcaaaagttg tcgtttaatc
     4201 aattaatttt gatgtttgat caactgacgg aagcacataa gaaattgaat caaaatgtaa
     4261 atccaacgct tgtatttgaa caaatcgtaa ttaagggtgt gagttagatg ccaaatgtaa
     4321 taggtgttca gtttcaaaaa gcgggaaaat tagaatatta tacacctaat gatatacaag
     4381 tagatataga tgactgggta gttgtcgaat ctaaaagagg catagagata ggtattgtta
     4441 aaaatccatt aatggatatt gctgaagagg atgttgtgtt acctcttaaa aatattattc
     4501 gcgttgctga tgacaaagat attgataaat ttaattgtaa tgaacgagat gctgaaaatg
     4561 cattaatact atgtaaagac attgtaagag aacaaggttt ggacatgcgt ttagtcaatt
     4621 gcgaatatac attagataaa tcgaaagtta tttttaattt tacggcggat gatcgtattg
     4681 attttagaaa attagtaaaa atattagcgc aacatttaaa aacacgtatc gagttgagac
     4741 aaattggtgt aagggatgaa gccaaattgc ttggcggtat cggaccttgt ggtaggtcgt
     4801 tatgttgttc tacattttta ggagattttg aaccagtatc gattaagatg gctaaggatc
     4861 aaaatttatc attaaatcca actaaaattt ctggtgcatg tggtcgtttg atgtgttgtt
     4921 taaaatatga aaatgactat tatgaggaag tacgtgcaca attacctgat attggtgaag
     4981 caattgaaac gcctgatggt aacgggaaag tagttgcttt aaatatatta gacatttcta
     5041 tgcaggtgaa gcttgaggga catgaacagc cacttgaata taaattagaa gaaatagaaa
     5101 ctatgcatta aggaggcatt attacatttg gatcgcaatg aaatatttga aaaaataatg
     5161 cgtttagaaa tgaatgtcaa tcaactttca aaggaaacat cagaattaaa ggcacttgca
     5221 gttgaattag tagaagaaaa tgtagcgctt caacttgaaa atgataattt gaaaaaggtg
     5281 ttgggcaatg atgaaccaac tactattgat actgcgaatt caaaaccagc aaaagctgtg
     5341 aaaaagccat taccaagtaa agataatttg gctatattgt atggagaagg atttcatatt
     5401 tgtaaaggcg aattatttgg aaaacatcga catggtgaag attgtctgtt ctgtttagaa
     5461 gttttaagtg attaatcaag cacactcaaa tagtgttata attataaatg aatatggttt
     5521 ggataagtct gagacaatgc atgtttcagg ctttaattgt gtataaagtt ttggtgattg
     5581 cataagagat ggcggtacta aatgttatta ttaagtgtgc acgcagtatc attagttata
     5641 aaatgtagct gttaaaagtc aaaaatacat cgaatgtagt tagtcatata atataaaaag
     5701 agatttcaat tactcaatag aaaaaggttg tcttcatagg agttaaaaat gttaaaagag
     5761 aatgaacgat ttgatcaact aatcaaagaa gattttagta ttattcaaaa tgatgatgtt
     5821 ttttcgtttt caacggatgc tttgttgtta gggcatttta caaaacctag aacaaaagat
     5881 attgtgttgg acttatgttc aggcaatggg gtgataccgt tgttattgtt tgcgaaacat
     5941 ccacgacata tagaaggtgt tgagattcaa aaaacacttg tcgatatggc gcgacgcaca
     6001 tttcaattca atgatgttga tgaatatata acaatgcatc acatggattt gaaaaacgtt
     6061 actaaagtat ttaaaccttc acaatatact ttagtaacgt gtaatccacc ttattttaaa
     6121 gagaatcagc aacaccaaca tcaaaaagaa gcacataaga tagcgagaca tgagattatg
     6181 tgtacacttg aagattgcat gattgcagcc cgtcatttat taaaagaagg tggcaggcta
     6241 aacatggtac atcgtgcaga gagactaatg gatgtcttgt ttgaaatgag aaaagtgaat
     6301 attgaaccta agaaagtcgt ttttatatat agtaaagtag ggaaatcagc acaaacgata
     6361 gtagtagaag gtcgaaaagg tggaaatcaa ggtttagaaa tcatgccccc attttatatt
     6421 tataatgaag atggtaatta tagcgaagaa atgaaggaag tatattatgg atagtcattt
     6481 tgtatatatt gtaaaatgta gtgatggaag tttatataca ggatacgcta aagacgttaa
     6541 tgcacgtgtt gaaaaacata accgaggtca aggagccaaa tatacgaaag taagacgtcc
     6601 ggtgcattta gtttatcaag aaatgtatga gacaaagtct gaagcattga agcgtgaata
     6661 tgaaattaaa acttatacca gacaaaagaa attgcgatta attaaggagc gatagtatgg
     6721 ctgtattata tttagtgggc acaccaattg gtaatttagc agatattact tatagagcag
     6781 ttgatgtatt gaaacgtgtt gatatgattg cttgtgaaga cactagagta actagtaagc
     6841 tgtgtaatca ttatgatatt ccaactccat taaagtcata tcacgaacat aacaaggata
     6901 agcagactgc ttttatcatt gaacagttag aattaggtct tgacgttgcg ctcgtatctg
     6961 atgctggatt gcccttaatt agtgatcctg gatacgaatt agtagtggca gccagagaag
     7021 ctaatattaa agtagagact gtgcctggac ctaatgctgg gctgacggct ttgatggcta
     7081 gtggattacc ttcatatgta tatacatttt taggattttt gccacgaaaa gagaaagaaa
     7141 aaagtgctgt attagagcaa cgtatgcatg aaaatagcac attaattata tacgaatcac
     7201 cgcatcgtgt gacagataca ttaaaaacaa ttgcaaagat agatgcaaca cgacaagtat
     7261 cactagggcg tgaattgact aagaagttcg aacaaattgt aactgatgat gtaacacaat
     7321 tacaagcatt gattcagcaa ggcgatgtac cattgaaagg cgaattcgtt atcttgattg
     7381 agggtgctaa agcgaacaat gagatatcgt ggtttgatga tttatctatc aatgagcatg
     7441 ttgatcatta tattcaaact tcacagatga aaccaaaaca agctattaaa aaagttgctg
     7501 aagaacgaca acttaaaacg aatgaagtat ataatattta tcatcaaata agttaatcac
     7561 tttatcgatt atatgaaatt ttaaacgatt ttataaacgc aagctgtaat tttaaatggt
     7621 aagttatcat tttgtattga tactgataaa atgatgttga ctatgataaa aaaatgatga
     7681 catcgacgtt ttttaatgta aaataaatac attgaaagta ataaatacct taacattgaa
     7741 taagatgaaa atgagatgac gagataaatg ttcgcgtccg ttgaaatgca tagaaatctt
     7801 agatattatt tgaagtgaga cattacgagg aggaacagtt atggctaaag aaacatttta
     7861 tataacaacc ccaatatact atcctagtgg gaatttacat ataggacatg catattctac
     7921 agtggctgga gatgttattg caagatataa gagaatgcaa ggatatgatg ttcgttattt
     7981 gactggaacg gatgaacacg gtcaaaaaat tcaagaaaaa gctcaaaaag ctggtaagac
     8041 agaaattgaa tatttggatg agatgattgc tggaattaaa caattgtggg ctaagcttga
     8101 aatttcaaat gatgatttta tcagaacaac tgaagaacgt cataaacatg tcgttgagca
     8161 agtgtttgaa cgtttattaa agcaaggtga tatctattta ggtgaatatg aaggttggta
     8221 ttctgttccg gatgaaacat actatacaga gtcacaatta gtagacccac aatacgaaaa
     8281 cggtaaaatt attggtggca aaagtccaga ttctggacac gaagttgaac tagttaaaga
     8341 agaaagttat ttctttaata ttagtaaata tacagaccgt ctattagaat tctatgacca
     8401 aaacccagat tttatacaac caccatcaag aaaaaatgaa atgattaaca acttcattaa
     8461 accaggactt gctgatttgg ctgtttctcg tacatcattt aattggggtg tccatgttcc
     8521 gtctaatcca aaacatgttg tttatgtttg gattgatgcg ttagttaact atatttcagc
     8581 attaggctat ttatcagatg atgagtcact atttaacaaa tactggccag cagatattca
     8641 tttaatggct aaggaaattg tgcgattcca ctcaattatt tggcctattt tattgatggc
     8701 attagactta ccgttaccta aaaaagtctt tgcacatggt tggattttga tgaaagatgg
     8761 aaaaatgagt aaatctaaag gtaatgtcgt agaccctaat attttaattg atcgctatgg
     8821 tttagatgct acacgttatt atctaatgcg tgaattacca tttggttcag atggcgtatt
     8881 tacacctgaa gcatttgttg agcgtacaaa tttcgatcta gcaaatgact taggtaactt
     8941 agtaaaccgt acgatttcta tggttaataa gtactttgat ggcgaattac cagcgtatca
     9001 aggtccactt catgaattag atgaagaaat ggaagctatg gctttagaaa cagtgaaaag
     9061 ctacactgaa agcatggaaa gtttgcaatt ttctgtggca ttatctacgg tatggaagtt
     9121 tataagtaga acgaataagt atattgacga aacaacgcct tgggtattag ctaaggacga
     9181 tagccaaaaa gatatgttag gcaatgtaat ggctcactta gttgaaaata ttcgttatgc
     9241 agctgtatta ttacgtccat tcttaacaca tgcgccgaaa gagatttttg aacaattgaa
     9301 cattaacaat cctcaattta tggaatttag tagtttagag caatatggtg tgcttactga
     9361 gtcaattatg gttactgggc aacctaaacc tattttccca agattggata gcgaagcgga
     9421 aattgcatat atcaaagaat caatgcaacc gcctgctact gaagaggaaa aagaagagat
     9481 tcctagcaaa cctcaaattg atattaaaga ctttgataaa gttgaaatta aggcagcaac
     9541 gattattaat gctgaacatg ttaagaagtc agataagctt ttaaaaattc aagtagactt
     9601 agattctgaa caaagacaaa ttgtatcagg aattgccaaa ttctatacac cagatgatat
     9661 tattggtaaa aaagtagcag ttgttactaa cctgaaacca gctaaattaa tgggacaaaa
     9721 atctgaaggt atgatattat ctgctgaaaa agatggtgta ttaaccttag taagtttacc
     9781 aagtgcaatt ccaaatggtg cagtgattaa ataactgtat ttttaaaaat taggagagat
     9841 aattatgtta atcgatacac atgtccattt aaatgatgag caatacgatg atgatttgag
     9901 tgaagtgatt acacgtgcta gagaagcagg tgttgatcgt atgtttgtag ttggttttaa
     9961 caaatcgaca attgaacgcg cgatgaaatt aatcgatgag tatgattttt tatatggcat
    10021 tatcggttgg catccagttg atgcaattga ttttacagaa gaacacctgg aatggattga
    10081 atctttggct cagcatccaa aagtgattgg tattggtgaa atgggattag attatcactg
    10141 ggataaatct cctgcagatg ttcaaaagga agtttttaga aagcaaattg ctttagctaa
    10201 gcgtttgaag ttaccaatta tcattcataa ccgtgaagca actcaagact gtatcgatat
    10261 cttattggag gagcatgctg aagaggtagg cgggattatg catagcttta gtggttctcc
    10321 agaaattgca gatattgtaa ctaataagct gaatttttat atttcattag gaggacctgt
    10381 gacatttaaa aatgctaaac agcctaaaga agttgctaag catgtgtcaa tggagcgttt
    10441 gctagttgaa accgatgcac cgtatctttc gccacatccg tatagaggga agcgaaatga
    10501 accggcgaga gtaactttag tagctgaaca aattgctgaa ttaaaaggct tatcttatga
    10561 agaagtgtgc gaacaaacaa ctaaaaatgc agagaaattg tttaatttaa attcataaag
    10621 ttaaaagtga gaaagatcac cgccataaat gtaaacgatg ctatattcgt ttaatatcct
    10681 atggttcttt ctcacttttt taaattaaaa tatcgtgcat gtggaatacg tgcgatagag
    10741 atggttagag ctttgaaatt aagaattgta ggaaggcgtt ttaaatgaaa atcaatgagt
    10801 ttatagttgt agaaggacga gatgatactg agcgtgttaa acgagctgtt gaatgtgata
    10861 cgattgaaac gaatggtagt gccatcaacg aacaaacttt agaagtaatt agaaatgctc
    10921 aacaaagtcg aggcgttatt gtattaacag atccagattt cccaggagat aaaattagaa
    10981 gtacaattac tgaacatgtc aaaggtgtta aacatgcgta tattgataga gaaaaagcta
    11041 aaaataaaaa agggaaaatt ggtgttgaac atgccgactt aattgatatt aaagaagcgt
    11101 taatgcatgt tagttcaccc tttgatgaag cttatgaatc aattgataaa tctgtgctaa
    11161 tagagttggg gttaatcgtt gggaaagatg caaggcgccg tagagaaatt ttaagtagaa
    11221 aattgcgaat cggccattcc aatggtaagc agttattgaa aaagttaaat gcatttggtt
    11281 ataccgaagc ggatgtaagg caagctttag aagatgaatg aggaagtgaa aatgttggat
    11341 aataaagata ttgcaacacc atcaagaacg cgagcgttgt tagataaata tggctttaat
    11401 tttaaaaaaa gtttaggaca gaactttttg atagatgtga atatcattaa taatatcatt
    11461 gatgcaagtg atattgatgc acaaactggg gtgattgaaa ttggtccagg catggggtca
    11521 ttgacagaac aattggctag acatgctaaa agagtattgg catttgaaat tgatcaacgt
    11581 ttaatacctg tattaaatga tacactatca ccttatgata atgtgacggt gattaatgaa
    11641 gatattttaa aagcgaatat taaagaagct gttgaaaatc atttacaaga ttgcgaaaaa
    11701 ataatggttg ttgcaaacct gccgtactat attacgacgc caattttatt aaatttaatg
    11761 caacaagata taccaattga tggctacgtg gtgatgatgc aaaaagaagt gggcgaacgc
    11821 ttaaatgctg aagtaggttc aaaagcatat ggttcgttat caattgtcgt acaatactat
    11881 acagagacta gtaaagtatt aacggtacct aaatctgtat ttatgccacc acctaatgtt
    11941 gattcaatag ttgtaaaact gatgcagaga actgaaccgt tagtaacagt agataacgag
    12001 gaagcattct ttaagttagc aaaagcagca tttgcacaaa gaagaaagac aattaacaat
    12061 aactatcaaa attattttaa agatggtaaa caacacaaag aagtgatttt acaatggttg
    12121 gaacaagcag gtattgatcc aagacgtcgc ggtgaaacgc tatctattca agattttgct
    12181 aaattgtatg aagaaaagaa aaaattccct caattagaaa attaaatgat tgacaaagca
    12241 aagcactatt gttaaaattt aaattttgtt tgacgaaaac gctgcaaata tggtattatg
    12301 taacttgtag cgaggtggag caatatgcca aaatcaattt tggacatcaa aaattctatt
    12361 gattgtcatg taggaaatcg tattgtactg aaagccaatg gaggccgtaa gaaaacaata
    12421 aaacgttctg gaattttaaa agaaacatat ccgtcagttt tcattgttga gttagatcaa
    12481 gacaaacaca actttgagag agtatcttat acatacactg atgtgttaac tgaaaatgtt
    12541 caagtttcat ttgaagagga taatcatcac gaatcaattg cacactaaat aagacatata
    12601 gagatgttag acgtttctta gtataagaag taaatattat gataattatt tgagtgttgg
    12661 gcgttatgtt caatactctt tttatttaca aaatgtttaa cactgatgtt tcgcttatag
    12721 atttttcagt aaatggatac ttgtatttat aaacacaaat acaagtaaat actaagtaat
    12781 tagatggaga aaattacttt tttattaaaa aaacactaaa aaacaaatta aaatgtcaaa
    12841 tattaattct ctttatgtta aaatcatcat attaagataa cgaaaagagg gcggaaaatg
    12901 atatatgaaa cggcaccagc caaaattaat tttacgctcg atacactttt taaaagaaat
    12961 gatggctatc atgagattga aatgataatg acaacagttg atttaaatga tcgtttaact
    13021 tttcataaaa gaaaagatcg aaagatagtt gttgagattg aacataatta tgtgccttct
    13081 aatcataaaa atctcgcata tcgtgcagcg caactattta ttgagcaata tcaactaaag
    13141 caaggtgtaa caatttctat cgataaagaa atacctgttt ctgctggctt agctggaggt
    13201 tcggctgatg cagcagcaac gttaagagga ttgaatcgac tttttgatat aggggcgagt
    13261 ttggaagaat tggctctact aggcagtaaa atcgggacag atattccgtt ttgtatttat
    13321 aataaaactg cactatgtac tggaagagga gagaaaatcg agtttttaaa taaaccacct
    13381 tcagcttggg tgattcttgc taaaccaaac ttaggcatat catcaccaga tatatttaag
    13441 ttgatcaatt tagataagcg ttacgacgta catacgaaaa tgtgttatga ggccttagaa
    13501 aatcgagatt atcaacaatt atgtcaaagt ttgtctaatc gattagagcc aatttctgtt
    13561 tcaaaacacc cacaaatcga taaattaaaa aataatatgt tgaaaagtgg tgcagatggt
    13621 gcgttaatga gtggaagcgg accgactgtg tatgggctag cacgaaaaga aagccaagca
    13681 aaaaatattt ataatgcagt taacggttgt tgtaatgaag tgtacttagt tagactatta
    13741 ggatagaagg gttgaaaaga tgagatataa acgaagcgag agaattgttt ttatgacgca
    13801 atatttgatg aaccatccga ataaattgat tccattaact ttttttgtga aaaaatttaa
    13861 acaggcgaag tcttcaataa gtgaagatgt ccaaattata aaaaatacat tccaaaaaga
    13921 aaagttaggt acagtaatta ctactgctgg cgcaagtggt ggtgttacgt ataaaccaat
    13981 gatgagtaaa gaagaggcga ctgaagttgt taatgaggtc attactctat tagaagagaa
    14041 agaacgtttg ttacctggcg ggtatttatt tttatcagat ttggtaggta atccatcgct
    14101 actaaacaaa gttggtaagt taattgccag tatttacatg gaagaaaaat tagatgctgt
    14161 tgttaccatt gcgacaaaag gtatttcatt ggcaaatgcg gttgctaata ttttaaattt
    14221 accagtagta gtgattagaa aagacaacaa ggtgactgaa ggttctacag tttcaattaa
    14281 ttacgtttca ggatcttcaa gaaaaataga gacaatggta ctttcgaaga gaactttagc
    14341 agaaaattca aatgttttag ttgtcgatga ttttatgagg gctggtggct ctattaatgg
    14401 tgttatgaat ttaatgaatg agtttaaagc ccatgtaaaa gggatatcag tacttgtaga
    14461 atcaaaagaa gttaaacaaa gattgattga agattatact tccttagtga aattatctga
    14521 tgtagatgaa tataatcaag agtttaacgt agaacctggc aacagtttat ctaagttttc
    14581 ataaaaggag ttttagtatt atgaaaatca ttaacacaac aagattaccg gaagcacttg
    14641 gaccatattc gcatgcaaca gttgtgaatg gtatggttta tacttctggt cagattccat
    14701 tgaatgttga tggggaaatc gtaagcgctg atgttcaagc acagacaaaa caagttttag
    14761 aaaatttaaa ggttgttttg gaagaagcag gatctgattt gaattctgtt gcgaaagcga
    14821 ccattttcat taaagatatg aatgatttcc aaaaaataaa tgaagtgtat ggtcaatatt
    14881 ttaatgaaca caagccagcg cgtagttgtg tagaggttgc gcgtttgcca aaagatgtga
    14941 aagtagaaat tgaattagta agtaaaatta aggaattata attttcgatt aatatgttta
    15001 atcaagcttc taaataaaac agagagatat atactatagg ggggctcact acatgaaagt
    15061 gacagatgta agacttagaa aaatacaaac agatggacga atgaaagcac tcgtttccat
    15121 tacattagat gaagctttcg taattcatga tttacgtgta attgaaggaa actctggctt
    15181 gttcgttgca atgccaagta aacgtacacc agatggtgaa ttccgcgaca tcgcgcatcc
    15241 tattaattca gatatgagac aagaaattca agatgcagtg atgaaagtat atgatgaaac
    15301 agatgaagta gtaccagata aaaacgctac atcagaagat tcagaagaag cttaatcaat
    15361 tttatattta gcgatgtaat acatttgcaa taagttgatt tgatactgtc gataaagcat
    15421 aaagctttgt cggcagtttt tttagtttgt attaatgttt ttttattttt aatgaaaggc
    15481 taataaatat atacgttaac agattatgat gatatgaaaa ttattgattc attgctgata
    15541 aaaattgtgt ttgaatgatg ctcgtatttt tgaagtaaga aaaaagttgt ttttaaaatt
    15601 acaacgaatt aaaaacaatg ccttttatat gttgaaagag tattgcagat taaattataa
    15661 taatgacgaa gtgtaaaaat taatgggggt taatgttcat gcgaagacac gcgataattt
    15721 tggcagcagg taaaggcaca agaatgaaat ctaaaaagta taaagtgcta cacgaggttg
    15781 ctgggaaacc tatggtcgaa catgtattgg aaagtgtgaa aggctctggt gtcgatcaag
    15841 ttgtaaccat cgtaggacat ggtgctgaaa gtgtaaaagg acatttaggc gagcgttctt
    15901 tatacagttt tcaagaggaa caactcggta ctgcgcatgc agtgcaaatg gcgaaatcac
    15961 acttagaaga caaggaaggt acgacgatcg ttgtatgtgg tgacacaccg ctcatcacaa
    16021 aggaaacatt agaaacattg attgcgcatc atgaggatgc taatgctcaa gcaactgtat
    16081 tatctgcatc gattcaacaa ccatatggat acggaagaat cgttcgaaat gcgtcaggtc
    16141 gtttagaacg catagttgaa gagaaagatg caacgcaagc tgaaaaggat attaatgaaa
    16201 ttagttcagg tatttttgcg tttaataata aaacgttgtt tgaaaaatta acacaagtga
    16261 aaaatgataa tgcgcaaggt gaatattacc tccctgatgt attgtcgtta attttaaatg
    16321 atggcggcat cgtagaagtc tatcgtacca atgatgttga agaaatcatg ggtgtaaatg
    16381 atcgtgtaat gcttagtcag gctgagaagg cgatgcaacg tcgtacgaat cattatcaca
    16441 tgctaaatgg tgtgacaatc atcgatcctg acagcactta tattggtcca gacgttacaa
    16501 ttggtagtga tacagtcatt gaaccaggcg tacgaattaa tggtcgtaca gaaattggcg
    16561 aagatgttgt tattggtcag tactctgaaa ttaacaatag tacgattgaa aatggtgcat
    16621 gtattcaaca gtctgttgtt aatgatgcta gcgtaggagc gaatactaag gtcggaccgt
    16681 ttgcgcaatt gagaccaggc gcgcaattag gtgcagatgt taaggttgga aattttgtag
    16741 aaattaaaaa agcagatctt aaagatggtg ccaaggtttc acatttaagt tatattggcg
    16801 atgctgtaat tggtgaacgt actaatattg gttgcggaac gattacagtt aactatgatg
    16861 gtgaaaataa atttaaaact atcgtcggca aagattcatt tgtaggttgc aatgttaatt
    16921 tagtagcacc tgtaacaatt ggtgatgatg tattggtggc agctggttcc acaatcacag
    16981 atgacgtacc aaatgacagt ttagctgtgg caagagcaag acaaacaaca aaagaaggat
    17041 ataggaaata atcatttacg tatttaaaat gactaggata aaaggataat cctatgtaat
    17101 attaatgtaa tctttatgat ttaatgattc acatagtaat ggagttacat tttatatata
    17161 atagtaattg cgtaagtaaa taattggagg actataaatg ttaaataatg aatataagaa
    17221 ttcgtcatta aagatttttt cattgaaagg aaacgaagca ttagcgcaag aagttgctga
    17281 ccaagtagga attgaactag gtaaatgttc agttaaacgt tttagtgatg gagaaattca
    17341 aattaatatc gaagagagta ttcgtggttg tgacgtattt attattcaac caacatcata
    17401 tcctgtgaat ctacatttaa tggaattatt aattatgatt gacgcttgta aacgtgcttc
    17461 tgcagcaaca atcaatattg tagtgccata ttatggatat gcaagacaag atagaaaagc
    17521 ccgtagccgt gagccaatca cagctaaatt agttgcaaac ttaatcgaaa cagctggcgc
    17581 aactcgtatg attgcgttag acttacatgc accacaaatt caaggattct ttgatattcc
    17641 aattgaccac ttaatgggtg tgccaattct tgctaaacat ttcaaagatg atccgaatat
    17701 taacccagaa gaatgtgtcg ttgtttcacc agaccatggc ggtgttacac gtgcacgtaa
    17761 attagctgac attttaaaaa ctccaattgc aattatagat aaacgtcgtc ctagaccaaa
    17821 tgttgctgaa gtgatgaaca ttgttggtga gattgaagga cgtacggcaa ttattattga
    17881 cgatattatt gatacagcag gtacaatcac tttagctgca caagcattaa aagataaagg
    17941 tgctaaagaa gtatatgctt gttgtacaca ccctgtttta tcaggaccgg ctaaagaacg
    18001 tatcgaaaat tctgctataa aagaattaat cgtaacaaac tcaattcatt tagatgaaga
    18061 ccgcaaacca tctaacacta aagaattatc tgttgctggt ttaatcgcac aagctatcat
    18121 tcgtgtatac gaaagagaat cagttagcgt attatttgac taatatttaa aaggcgtttg
    18181 acgaacatat tccaaacgtg tataatagtt tcgttcgtga ttatacgaat aaataaacac
    18241 ttgcaagcaa cgatgatgtt gatgggtaag tgaggtgctc gttttgagca aaaatgaaag
    18301 gtggaaatga gaatggcttc attaaagtca atcatccgtc aaggtaaaca aacacgttca
    18361 gatcttaaac aattaagaaa atctggtaaa gtaccagcag tagtatacgg ttacggtact
    18421 aaaaacgtgt cagttaaagt tgatgaagta gaattcatca aagttatccg tgaagtaggt
    18481 cgtaacggtg ttatcgaatt aggcgttggt tctaaaacta tcaaagttat ggttgcagac
    18541 taccaattcg atccacttaa aaaccaaatt actcacattg acttcttagc aatcaatatg
    18601 agtgaagaac gtactgttga agtaccagtt caattagttg gtgaagcagt aggcgctaaa
    18661 gaaggcggcg tagttgaaca accattattc aacttagaag taactgctac tccagacaat
    18721 attccagaag caatcgaagt agacattact gaattaaaca ttaacgacag cttaactgtt
    18781 gctgatgtta aagtaactgg cgacttcaaa atcgaaaacg attcagctga atcagtagta
    18841 acagtagttg ctccaactga agaaccaact gaagaagaaa tcgaagctat ggaaggcgaa
    18901 caacaaactg aagaaccaga agttgttggc gaaagcaaag aagacgaaga aaaaactgaa
    18961 gagtaatttt aatctgttac attaaagttt ttatactttg tttaacaagc actgtgctta
    19021 ttttaatata agcatggtgc tttttgtgtt attataaagc ttaattaaac tttattgctt
    19081 tgtaataaag tttaattaat tttagtgagt aaaagacatt aaactcaaca atgatacatc
    19141 ataaaaattt taatgtactc gattttaaaa tacatactta ctaagctaaa gaataatgat
    19201 aattgatggc aatggcggaa aatagatgtt gtcattataa taataaatga aacaattatg
    19261 ttggaggtaa acacgcatga aatgtattgt aggtctaggt aatataggta aacgttttga
    19321 acttacaaga cataatatcg gctttgaagt cgttgattat attttagaga aaaataattt
    19381 ttcattagat aaacaaaagt ttaaaggtgc atatacaatt gaacgaatga acggcgataa
    19441 agtgttattt atcgaaccaa tgacaatgat gaatttgtca ggagaagcag ttgcaccgat
    19501 tatggattat tacaatgtta atccagaaga tttaattgtc ttatatgatg atttagattt
    19561 agaacaagga caagttcgct taagacaaaa aggaagtgcg ggcggtcaca atggtatgaa
    19621 atcaattatt aaaatgcttg gtacagacca atttaaacgt attcgtattg gtgtgggaag
    19681 accaacgaat ggtatgacgg tacctgatta tgttttacaa cgcttttcaa atgatgaaat
    19741 ggtaacgatg gaaaaagtta tcgaacacgc agcacgcgca attgaaaagt ttgttgaaac
    19801 atcacgattt gaccatgtta tgaatgaatt taatggtgaa gtgaaataat gacaatattg
    19861 acaacgctta taaaagaaga taatcatttt caagacctta atcaggtatt tggacaagca
    19921 aacacactag taactggtct ttccccgtca gctaaagtga cgatgattgc tgaaaaatat
    19981 gcacaaagta atcaacagtt attattaatt accaataatt tataccaagc agataaatta
    20041 gaaacagatt tacttcaatt tatagatgct gaagaattgt ataagtatcc tgtgcaagat
    20101 attatgaccg aagagttttc aacacaaagc cctcaactga tgagtgaacg tattagaact
    20161 ttaactgcgt tagctcaagg taagaaaggg ttatttatcg ttcctttaaa tggtttgaaa
    20221 aagtggttaa ctcctgttga aatgtggcaa aatcaccaaa tgacattgcg tgttggtgag
    20281 gatatcgatg tggaccaatt tcttaacaaa ttagttaata tggggtacaa acgggaatcc
    20341 gtggtatcgc atattggtga attctcattg cgaggaggta ttatcgatat ctttccgcta
    20401 attggggaac caatcagaat tgagctattt gataccaaaa ttgattctat tcgggatttt
    20461 gatgttgaaa cgcagcgttc caaagataat gttgaagaag tcgatatcac aactgcaagt
    20521 gattatatca ttactgaaga agtgatcagc catcttaaag aagagttaaa aactgcatat
    20581 gaaaatacaa gacccaaaat agataaatca gtgcgcaatg atttgaaaga aacgtatgaa
    20641 agctttaaat tattcgaaag tacatacttt gatcatcaaa tactacgtcg cttagtagcg
    20701 tttatgtatg aaacaccttc gacaattatt gagtatttcc aaaaagatgc aatcattgca
    20761 gttgatgaat ttaatcgtat taaagaaact gaagaaagtt taacagtaga gtctgattcg
    20821 tttattagca atattattga aagtggtaat ggatttatag gacaaagttt tataaaatat
    20881 gatgattttg aaacattgat tgaaggctat cctgtcactt atttttcatt attcgctaca
    20941 acaatgccga taaaactaaa tcatattatt aaattttcat gtaaacctgt tcaacaattt
    21001 tatgggcaat atgacattat gcgttctgaa tttcaacgat atgttaatca aaactatcat
    21061 atcgtggttt tggtcgaaac cgaaactaaa gttgaacgta tgcaagcgat gttaagtgaa
    21121 atgcatattc catcaataac aaaattgcat cgctcaatgt catcgggaca agcagtgatt
    21181 attgagggca gtttatctga aggatttgaa ctacctgata tgggattagt tgtcattact
    21241 gagcgtgagc tttttaaatc aaaacagaaa aagcaacgaa aacgtacgaa agctatctca
    21301 aatgctgaaa aaattaagtc ttaccaagat ttaaatgtgg gagattatat tgttcatgtg
    21361 catcatggtg ttggtagata tttaggtgtt gagacgctcg aagtggggca aacgcatcgt
    21421 gattatatta aattgcaata taaaggtacg gatcaactat ttgttccagt agatcaaatg
    21481 gatcaagttc aaaaatatgt agcttcggaa gataagacgc caaaattaaa taaactcggt
    21541 ggcagtgaat ggaaaaaaac aaaagctaaa gttcaacaaa gtgttgaaga tattgctgaa
    21601 gagttgattg atttatataa agaaagagaa atggcagaag gttatcaata tggggaagac
    21661 acagctgagc aaacaacatt tgaattagat tttccatatg aacttacgcc tgaccaagct
    21721 aaatctatcg atgaaattaa agatgacatg caaaaatcgc gtccaatgga tcgcttgcta
    21781 tgtggtgatg ttggttatgg taaaactgaa gttgcagtga gagcagcatt caaagctgta
    21841 atggaaggaa agcaggttgc atttttagtt cctacaacta ttttagctca gcaacattat
    21901 gagacgttaa ttgagcgtat gcaagatttt cctgttgaaa ttcaattaat gagtcgtttt
    21961 agaacgccta aagagataaa acaaactaag gaaggactta aaactggatt tgttgacata
    22021 gttgttggta cacacaaatt acttagtaaa gatatacagt ataaagattt agggctgttg
    22081 attgtagatg aagaacaacg atttggtgta cgccataaag agcgtattaa aacattaaaa
    22141 cataatgtag atgtactaac attgactgca accccaatac ctagaacatt gcatatgagt
    22201 atgctaggtg tgcgcgattt gtcagtgatt gaaacgccgc cagaaaatcg tttcccagtt
    22261 caaacatatg tattagaaca gaacatgagt tttatcaaag aagctttaga aagagaacta
    22321 tcccgtgatg gccaagtgtt ttatctttat aataaagtgc aatccattta tgaaaaacga
    22381 gaacaactcc agatgttaat gccagatgct aacattgcag ttgctcatgg acaaatgaca
    22441 gagcgcgatt tagaagaaac gatgttaagt tttatcaata atgaatatga tattttagta
    22501 acgacgacga ttattgaaac aggtgtcgat gtcccaaatg caaatacttt gatcattgaa
    22561 gatgcagatc gctttggatt gagtcagttg tatcaattaa gaggtcgtgt tggtcgttca
    22621 agtcgtattg gttatgcata cttcttacat ccagcaaata aggtactaac tgagactgca
    22681 gaagatcgat tacaagcgat taaagaattt acggagttag gctcaggatt taagattgcg
    22741 atgcgtgatt tgaacattcg tggtgctggt aatttgttag gtaaacaaca gcacggcttt
    22801 attgatacag ttggatttga tttgtacagt caaatgttag aagaagctgt aaatgaaaaa
    22861 cgtggtatta aggaaccaga atctgaggtg ccagaagtcg aagttgattt aaacttggat
    22921 gcatatttgc cgacagaata tattgcaaat gaacaagcta aaattgaaat ttataaaaag
    22981 ctacgaaaaa ctgaaacatt tgatcaaatt atcgacatta aagatgaatt aattgatcgt
    23041 tttaatgatt atcctgttga agtagcacgt ttgcttgata tagtggaaat aaaagtacac
    23101 gcattacatt caggtatcac gttgattaaa gataaaggga aaataattga tattcattta
    23161 tctgtaaaag ccactgaaaa tattgatggc gaagtgctgt tcaaagcaac acaaccttta
    23221 ggtagaacaa tgaaggttgg tgttcaaaat aatgcaatga caattacttt aacgaaacaa
    23281 aatcaatggc ttgatagttt gaagttttta gttaagtgca ttgaagaaag tatgagaatc
    23341 agtgatgaag cataaagaag catttaatgg cgttgtcgtg ttaactgctg cattaattgt
    23401 cattaaaatt ctgagtgctg tatatcgaat tccatatcaa aatatattag gtgatacagg
    23461 tttgtatgca tatcaacaag tgtatccaat tgtagcatta ggaatgatat tatcgatgaa
    23521 tgccattcct agtgcaatta cacaaaatat agggaagtat catagtgacg aagcatatgc
    23581 aaaagcggtc gcttatatac aattagttgg tatattatta tttattgcta tttttgtgtt
    23641 tgcgaacaat attgcacata tgatgggtga tagccattta acaccaatga ttcaagcagc
    23701 aagtttaagc tttatattta taggtatgct tggcgtgtta agaggttatt atcaatctgc
    23761 aaataatatg acagttccgg ctatttccca ggttatagaa caagttatac gagtaggtat
    23821 tatcattgtt actattgtta tttttgtaga cagaggttgg acgatatatg aagcgggaac
    23881 aattgctatt ttagcatcaa cgataggttt tttaggttct tcaatttatt tagtagcgca
    23941 ccgacctttt aagtttaaaa tggtaaataa cactgcaaag attgtttgga aacagttcgc
    24001 actttcggtt ttgattttcg ctatcagtca attaatcgta attttatggc aagtgattga
    24061 tagtgttact attattaagt cacttcaagc gatacgcgtg ccattcgatg ttgccataac
    24121 tgaaaaagga gtctatgacc gtggtgcatc atttattcag atgggattga ttgtaactac
    24181 aacatttagt tttgcgctca ttcctctgtt aagtgacgca atcaaaatga ataatcaggt
    24241 acttatgaat cgttatgcaa atgcgtcatt aaagattacg attttaataa gtacagcagc
    24301 gggaatagga ttaattaatt tattgccttt aatgaacggt gtgtttttta agacgaatga
    24361 tttaacctta acgttaagtg tttatatgat tacggtcatt tgtgtatcgt taattatgat
    24421 ggatatggct ttattacaag cgcaacatgc tgtgagacct atttttgttg gtatgacggc
    24481 aggattggtt attaaattta tacttaatat cattttgatt cgtttaagtg gcattattgg
    24541 tgcgagcatt agtactgttg tatcattaat tatattcggt acgattatcc atattgctgt
    24601 cacgagaaaa taccacttac atgcgatgag acgatttttt atcaatgttg ttttaggtat
    24661 ggtatttatg tcgattgttg ttcaatgcgt gttaaacata gtgacaacac acggtagatt
    24721 cactggactc attgaattat tatgtgcagc agtattaggt atcattgcat tgtttttcta
    24781 tatttttaga tttaatgttt tgacatataa agagttaact tatttaccat ttggttcaaa
    24841 gttgtatcaa attaagaaag gaagacgttg atggcacata ccattacgat tgttggctta
    24901 ggaaactatg gcattgatga tttgccgcta gggatatata aatttttaaa gacacaagat
    24961 aaagtttatg caagaacgtt agatcatcca gttatagaat cattgcaaga tgaattaaca
    25021 tttcagagtt ttgaccatgt ttatgaagca catgaccaat ttgaagatgt ctataatgat
    25081 attgtggcgc aattggttga agctgctaat gaaaaagata ttgtctatgc ggttccgggt
    25141 catcctagag ttgctgagac aactacagtg aaattactgg ctttagcaaa ggacaatact
    25201 gatatagatg tgaaagtttt aggtggtaaa agctttattg atgatgtgtt tgaagcagtt
    25261 aatgtagatc caaatgatgg cttcacactg ttagatgcga catcattaca agaagtaaca
    25321 cttaatgtta gaacgcatac attgattaca caagtttata gtgcaatggt tgctgctaat
    25381 ttgaaaatca ctttaatgga acgatatcct gatgattacc ctgttcaaat tgtcactggt
    25441 gcacgaagcg atggtgcgga taacgttgtg acatgcccat tatatgaatt ggatcatgat
    25501 gaaaatgcat tcaataattt gacgagtgta ttcgtaccaa aaatcataac atcgacatat
    25561 ttgtatcatg actttgattt tgcaacggaa gtgattgata ctttagttga tgaagataaa
    25621 ggttgtccat gggataaagt gcaaacgcat gaaacgctta agcgttattt acttgaagaa
    25681 acatttgaat tgttcgaagc tattgacaat gaagatgatt ggcatatgat tgaagagcta
    25741 ggagatattt tattacaagt gttattgcat actagtattg gtaaaaaaga agggtatatc
    25801 gacattaaag aagtgattac aagtcttaat gctaaaatga ttcgtagaca cccacacata
    25861 tttggtgatg ccaatgctga aactatcgat gacttaaaag aaatttggtc taaggcgaaa
    25921 gatgctgaag gtaaacagcc aagagttaaa tttgaaaaag tatttgcaga gcatttttta
    25981 aatttatatg agaagacgaa ggataagtca tttgatgagg cctcgttaaa gcagtggcta
    26041 gaaaaagggg agagtaatac atgagattag ataaatattt aaaagtatca cggttaataa
    26101 agcgacgtac gctagcaaaa gaagtaagtg atcaaggtag aattacaata aatggtaatg
    26161 ttgctaaagc tggatcggat gttaaagttg aagatgtgct gacgattcgc tttggtcaaa
    26221 aattagtaac agttaaagta actgcattaa atgaacatgc atctaaagat aacgcgaagg
    26281 gcatgtatga aatcattgaa gagcgtcgac ttgaagaagc gtaaattgga ggtgacaagc
    26341 aatgaaaaat aaagtagaac atatagaaaa tcagtacacg tcgcaagaga ataagaaaaa
    26401 acaacgtcaa aaaatgaaaa tgcgtgttgt tcgtaggcgt attacagtat ttgcgggcgt
    26461 attacttgcg ataattgttg ttttatcaat cttgcttgtt gtccaaaaac atcgcaatga
    26521 tatcgatgca caggagcgaa aagcgaaaga agcacagttt caaaagcaac aaaatgaaga
    26581 aattgcgtta aaagaaaagt tgaataatct gaatgacaaa gattatattg aaaaaattgc
    26641 gcgtgatgat tattacttaa gcaacaaagg tgaagtgatt tttaggttgc cagaagacaa
    26701 agattcgtct agctcaaaat cttcgaaaaa ataaatccaa attgattcaa aattatccga
    26761 gtatagacat tgtgaaaaaa tccaaacaag gatataataa gggaaaatcg aatcaaatcg
    26821 ggaggattta tttaacatat gtcaatcgaa gttggaaata agcttaaagg taaagtcact
    26881 ggtattaaaa agtttggtgc attcgtagaa ttacctgaag gaaaaagtgg tttagttcac
    26941 attagtgaag tcgcagataa ttatgttgaa aacgtagaag agcacctttc tgttggtgat
    27001 gaagtagacg taaaagtatt atctattgct gatgatggaa aaattagtct ttcaattaag
    27061 aaagctaaag accgtccacg tagacaacat acgagtaaac caagtcatca aaaaccagtg
    27121 caaaaagccg aagattttga aaagaaatta agcaatttct taaaagatag tgaagataaa
    27181 ttaacttcaa tcaaacgtca aacagaatct agacgcggtg gcaaaggttc aagacgttaa
    27241 ttaaaataaa taaagactgt ttcgataagg aatatattta gaatgatgcg tatcgaataa
    27301 tcgattgcag cgttagacaa tctaagactg tttcttaaat aaggagcagt ctcttttatt
    27361 tgtaatgata taactaagac ttataccatt tttgaaaatt gtaaaagtga ggtgatgtta
    27421 tgcagttaaa tagtaatggt tggcatgttg atgaccatat tgttgtcgct gtttctacag
    27481 gtattgatag tatgtgttta ttgtatcaac tactaaatga ttataaagat agttatagaa
    27541 aactaacatg cttacatgtc aatcatggcg ttaggtcagc ttcaatcgag gaagccagat
    27601 ttttagaagt atactgcgaa cgtcatcaca tcgatttaca tatcaaaaag ttagatttgt
    27661 cgcatagtct cgaccgaaat aacagcattc agaatgaagc tcgaattaaa cgttacgaat
    27721 ggtttgatga aatgatgaat gtattagaag cggatgtatt gctaacggcg catcatttgg
    27781 acgatcaatt agaaactatt atgtatcgta tttttaatgg gaaatcaacg cgtaataaac
    27841 taggatttga tgagttatcg aagcgaaaag gttatcagat ttatcgacca cttttagctg
    27901 tctctaaaaa agaaataaaa caattccaag agagatatca tattccatat tttgaagatg
    27961 aatctaataa agataacaaa tatgttagaa atgatattcg taatagaatt attccagcta
    28021 ttgatgaaaa taatcaactt aaagcatcgc atttattaaa attaaaacaa tggcatgatg
    28081 aacaatatga tattttgcaa tattcagcta aacaatttat tcaagaattt gtgaagtttg
    28141 atgaacagtc aaaatattta gaggtttcta gacaagcttt taataactta ccaaactcat
    28201 taaagatggt tgtgttggat tgcctactat caaagtatta tgagttgttt aatattagtg
    28261 ctaaaacata cgaagagtgg tttaaacaat ttagtagtaa gaaagcacaa ttcagtatta
    28321 atctcacgga taaatggata attcaaattg catatggtaa attaataata atggctaaaa
    28381 ataatggcga tacatatttt agagttcaaa ctattgaaaa gccaggtaat tatattttta
    28441 acaaatatcg attagagata cattctaatt taccaaaatg tttatttccg cttacagtga
    28501 gaacacgaca aagtggcgat acatttaaac tgaatgggcg cgatggttat aagaaagtga
    28561 atcgcctgtt tatagattgt aaagtgcaac agtgggttcg ggatcaaatg ccaatcgtat
    28621 tggataaaca acagcgcatt attgcggtag gagatttata tcaacaacaa acaataaaac
    28681 aatggattat aattagtaaa aatggagatg aatagcgtta tgcataatga tttgaaagaa
    28741 gtattgttaa ctgaagaaga tattcaaaat atctgtaagg aattgggagc acaattaaca
    28801 aaggattatc aaggtaaacc attagtatgc gtgggtatct taaaaggctc agcaatgttt
    28861 atgtcagatt taattaaacg aattgatacc catttatcaa ttgatttcat ggatgtttct
    28921 agttatcacg gaggcactga gtcaactggt gaagttcaaa tcattaaaga tttaggttct
    28981 tctattgaaa ataaagacgt attaattatt gaagatatct tagagactgg tactacactt
    29041 aagtcaatta ctgaattatt acaatctaga aaagttaatt cattagaaat agttacttta
    29101 ttagataaac caaaccgtcg taaagcggac attgaagcta agtatgtagg taaaaaaata
    29161 ccagatgaat ttgttgttgg ttacggttta gattatcgtg aattataccg aaacttacca
    29221 tatatcggta cgttaaaacc tgaagtgtat tcaaattaat tttttaatca atttcagtta
    29281 ttattactat gcgtttgaga aataatag
//
LOCUS       DAMZLE010000017        29099 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMZLE010000017 DAMZLE010000000
VERSION     DAMZLE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.017"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            264..1682
                     /locus_tag="POB92_000744"
     CDS             264..1682
                     /locus_tag="POB92_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI6578264.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLISLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(2095..3003)
                     /gene="rarD"
                     /locus_tag="POB92_000745"
     CDS             complement(2095..3003)
                     /gene="rarD"
                     /locus_tag="POB92_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDI6578265.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDEIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFNIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3225..3800
                     /locus_tag="POB92_000746"
     CDS             3225..3800
                     /locus_tag="POB92_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6578266.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            3966..4982
                     /locus_tag="POB92_000747"
     CDS             3966..4982
                     /locus_tag="POB92_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDI6578267.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            5225..6019
                     /locus_tag="POB92_000748"
     CDS             5225..6019
                     /locus_tag="POB92_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDI6578268.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPALMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPIHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTNPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKLAVTKNNYKHFLEKYFGLDITINRIEKQ"
     gene            complement(6153..6665)
                     /locus_tag="POB92_000749"
     CDS             complement(6153..6665)
                     /locus_tag="POB92_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDI6578269.1"
                     /translation="MAKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6948..7706
                     /gene="vraD"
                     /locus_tag="POB92_000750"
     CDS             6948..7706
                     /gene="vraD"
                     /locus_tag="POB92_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDI6578270.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7696..9576
                     /gene="vraE"
                     /locus_tag="POB92_000751"
     CDS             7696..9576
                     /gene="vraE"
                     /locus_tag="POB92_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDI6578271.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQTIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            9669..9860
                     /gene="vraH"
                     /locus_tag="POB92_000752"
     CDS             9669..9860
                     /gene="vraH"
                     /locus_tag="POB92_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDI6578272.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            10253..10453
                     /locus_tag="POB92_000753"
     CDS             10253..10453
                     /locus_tag="POB92_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6578273.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(10571..11140)
                     /locus_tag="POB92_000754"
     CDS             complement(10571..11140)
                     /locus_tag="POB92_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6578274.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(11400..11795)
                     /locus_tag="POB92_000755"
     CDS             complement(11400..11795)
                     /locus_tag="POB92_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDI6578275.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(11863..12216)
                     /locus_tag="POB92_000756"
     CDS             complement(11863..12216)
                     /locus_tag="POB92_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDI6578276.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(12533..13372)
                     /gene="noc"
                     /locus_tag="POB92_000757"
     CDS             complement(12533..13372)
                     /gene="noc"
                     /locus_tag="POB92_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDI6578277.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(13415..14134)
                     /gene="rsmG"
                     /locus_tag="POB92_000758"
     CDS             complement(13415..14134)
                     /gene="rsmG"
                     /locus_tag="POB92_000758"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI6578278.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(14134..16011)
                     /gene="mnmG"
                     /locus_tag="POB92_000759"
     CDS             complement(14134..16011)
                     /gene="mnmG"
                     /locus_tag="POB92_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI6578279.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(16078..17457)
                     /gene="mnmE"
                     /locus_tag="POB92_000760"
     CDS             complement(16078..17457)
                     /gene="mnmE"
                     /locus_tag="POB92_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI6578280.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(17596..17949)
                     /gene="rnpA"
                     /locus_tag="POB92_000761"
     CDS             complement(17596..17949)
                     /gene="rnpA"
                     /locus_tag="POB92_000761"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI6578281.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(18070..18207)
                     /gene="rpmH"
                     /locus_tag="POB92_000762"
     CDS             complement(18070..18207)
                     /gene="rpmH"
                     /locus_tag="POB92_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI6578282.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            18938..20299
                     /gene="dnaA"
                     /locus_tag="POB92_000763"
     CDS             18938..20299
                     /gene="dnaA"
                     /locus_tag="POB92_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI6578283.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            20577..21710
                     /gene="dnaN"
                     /locus_tag="POB92_000764"
     CDS             20577..21710
                     /gene="dnaN"
                     /locus_tag="POB92_000764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI6578284.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            22091..22336
                     /gene="yaaA"
                     /locus_tag="POB92_000765"
     CDS             22091..22336
                     /gene="yaaA"
                     /locus_tag="POB92_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDI6578285.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            22333..23445
                     /gene="recF"
                     /locus_tag="POB92_000766"
     CDS             22333..23445
                     /gene="recF"
                     /locus_tag="POB92_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI6578286.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            23455..25389
                     /gene="gyrB"
                     /locus_tag="POB92_000767"
     CDS             23455..25389
                     /gene="gyrB"
                     /locus_tag="POB92_000767"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI6578287.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            25426..28089
                     /gene="gyrA"
                     /locus_tag="POB92_000768"
     CDS             25426..28089
                     /gene="gyrA"
                     /locus_tag="POB92_000768"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDI6578288.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(28176..28988)
                     /locus_tag="POB92_000769"
     CDS             complement(28176..28988)
                     /locus_tag="POB92_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDI6578289.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         9723 a         5002 c         4772 g         9602 t
ORIGIN      
        1 tgcaaaatat atacagtata acgaatacaa gtgcatgaag ttaacttgta tcaattaatt
       61 tcttagtata ttagttcatc ttcaaaatct tttaaattta ataaatacca tctataccag
      121 ttcttcaccg attctcgaaa aataattata ttaatgattt cgttaattta attttatatt
      181 taattattac tgtacatctt ttgtagttag ctttattctt aaattgaaat atgtataaaa
      241 catagaaaga aggacgtttt aacatggaaa acacggttaa atatcgtaag tttatactcc
      301 ctatcgttgt aggtctcctt atttgggcac ttacaccttt taaaccggat gctgtggatc
      361 caacagcatg gtatatgttc gcaatattcg tcgcgacaat cattgcttgt attacacaac
      421 cgatgccaat tggggccgtc tctataattg gatttacaat catggtactc gttggcattg
      481 ttgacatgaa aacggctgtc gctggttttg gtaataatag catttggtta attgctatgg
      541 catttttcat ttcgagagga tttgtgaaaa caggtcttgg tagacgtatc gcacttcatt
      601 tcgtcaaatt atttggtaaa aaaacattag gcttagcata ttctatcgtc ggtgtagatt
      661 taattctagc gcctgctaca ccaagtaata ccgcgcgtgc cggtggaatc atgttcccaa
      721 ttatcaaatc actttctgaa tcatttggtt cgaaaccgaa agacggatca gcacgcaaaa
      781 tgggtgcatt tcttgttttc acagaattcc aaggtaattt aattactgcg gctatgtttt
      841 taactgcaat ggccggtaac ccccttgcac aaaatttagc atctagcaca tctaatgttc
      901 acattacatg gatgaattgg ttcctagctg ctttagttcc tggacttatt tccttaattg
      961 ttgtaccttt tattatttat aaaatttatc caccaactgt taaagaaaca ccaaatgcta
     1021 agagttgggc tgaaaatgaa ttagcgacta tgggtaaaat cgctttagct gaaaaattta
     1081 tgattggtat ttttgtcgtt gcgttaacac tatggattgt cggaagtttc attcatattg
     1141 atgcaacttt aacggccttt attgcgttag cattgttatt attgacaggc gtcttaacat
     1201 ggcaagacat tttaaacgaa acaggtgctt ggaacacatt agtatggttc tcagtattag
     1261 tgttaatggc cgaccaatta aacaagcttg gatttattcc ttggttaagt aaatccattg
     1321 ctacaagtct tggtggctta agctggccta tagtactggt cattttaata ttgttctact
     1381 tctattcaca ttacttattt gcaagttcta cagcacatat cagtgcgatg tatgcagcat
     1441 tactaggcgt tgccatcgca gccggtgcac caccattatt cagtgcatta atgttaggtt
     1501 tcttcggtaa cctattagct tcaacaacac actatagtag tggcccagcg ccgattctat
     1561 tctcttcagg ttacgtgact caaaaacgtt ggtggacaat gaacttgatc ttaggtttcg
     1621 tctactttat tatctggatt ggtttaggat cactttggat gaaagtaatt ggtatatttt
     1681 aaaatattta aattagcgct cgaatctcat tgatttgggc gctttttaat ttgtatttaa
     1741 aatcaacctt tgctaaatca agactccctt tttaaaatac gtttatcctt taaatcattg
     1801 cgtgcttcac taaaaatcta tataacgctt taaatcattg cgtaacatca cagaaaatac
     1861 atttctgtat aaacgcgctt cgttgattaa cattacgtat gccctcataa atcatgaaat
     1921 taaaaacacc ttcgtttaaa ttcattttaa gtgcgaattc aacgaaagtg atttatttca
     1981 tatttaatgt ttcaaattta tacagctgtc gctgttactg cacacatacc tcagtctcac
     2041 cttatcatga cacaaaggat attgaaaaca cgtataatta tctttcctcg tattttatag
     2101 ggtttttgcg accggatgtt tcttcaattt aatgtattga gaaagactat ataacacaat
     2161 acctgtccaa ataaatataa acgtaattaa ttgatctata ttaaaaggct ctttgaaaac
     2221 aaatatgccg agtacaaaca ttattgttgg tccaacgtat tgaataaatc ctattaacga
     2281 aagtggaata cgttttgccc cggctgagaa taggattagt ggtattgccg taatagcacc
     2341 agaaaataac aaccaaaatg atgacatgtt caatccaaat gacatctgat gttgctgcca
     2401 taaataaata acgtatatta gtccagcagg tgcggtaaca atacattcaa tcgtaatact
     2461 gctgatggca tcaatatgta ctactttttt caataatccg tatgtaccaa aggataacgc
     2521 taatataata gaaacgattg ggaattctcc aatcttgagc gtcatatata atacaccgat
     2581 gaatgcgaat aaaatagcta gccattcaaa tttattgaat ctttctttta aaaagataag
     2641 tgcgagcaaa atgctaacaa gtggatttat ataataacct aaacttgttt gtaggacgtg
     2701 accgttcgtt acagcccaaa taaatgtacc ccaatttaat gtaatgacat agcctgctac
     2761 gacaatcgct aatagctgaa tgggcttgcc taacaattga ttcatatctc gttgaaatgc
     2821 attgcgttgt ttttgtccaa ccgcgagtat gaaaatcatg aatattgctg aaaatataat
     2881 acgaaaggct aaaatttcaa atgcgcctat ttcatcaacg aactgccaat atataggtag
     2941 tattccccac agaatgtatg cactgagtgc taaaaatata ccttttttat attctgtatt
     3001 cacttacata cctccttact ctcccaaatt tttaacatgt tacgctcact tggttatgct
     3061 aatataacga ttttactaat aatatttcga taaagatatc attttgttta tatttcccac
     3121 atttattcac caaccactaa acaatattaa ttttataaat aattctgtac aaatcagggt
     3181 atattgccag aaagactacc atacaacata aaggatggat acaaatgact ttacctaaaa
     3241 ttggaaagcc tgcaacacgc gcgctaaatt cacaaggtat atacacatta gaagcagtat
     3301 cacaatatac gaagtcatct ctaatggaga tgcatggcgt tggtcctaaa gctatatcaa
     3361 tattggaaca agctttattt cagcaccaac tacattttaa aacggaagtt cagtcatcat
     3421 taccttttaa gttaacagga gatgtgtcat gcaatcatgc gcctaaacgt caacaaatga
     3481 tagattttat agttgctaca gcagcattag atattgaatt attacgttca ctggttacaa
     3541 cagaatttat ttggtctgtc cctggccgct ttgacatata cggacctcaa atattaattc
     3601 aagaactatc taatcattac aatcaggttg ctagtctcaa tatccattcc agcatcacac
     3661 atgggtgctt aggttccatg catggtattg aaatattaaa aactggtaaa gagattcatt
     3721 ttgctcattt ttttgaattt gaaaatcata aaaaggatgc caagttaagc aaagtgacat
     3781 cttacattgt tgttggttaa tttatcaaat tatatatatc gcaaaaaaat cttttgtggt
     3841 tacatgtata ttgttatata acaaataatt tggcataaca tttttgttat atgaacactt
     3901 attactagag tgcccattga acatacagta tgattaaata tcatgttatg aaagaaggat
     3961 atattttgac tgtttttaaa aatgagcgct taagctggtt accatacata gctattgtca
     4021 ttttgttaca cgttattggg tttagttttt tatggattgc tggaaaagac catcatatct
     4081 tatttggtat ggggattctt gcatatacat taggtttgcg tcatgcattt gatgcagatc
     4141 acattgctgc aatagataat acggttcgca aattattaca gcaacgcaaa gatccatctg
     4201 gtgtgggttt ctatttttca attggacatt catctgtcgt atttttaatg gctgtttttt
     4261 taggggtatc tgtaaaatgg gctaaagatg aattaccgca tttccaagat attggtggaa
     4321 cgattggtac actagtttca ggtttctttt tagtgcttat cggtgtgttg aatctaatta
     4381 ttttaatctc tttaattaac ttgttcgcta aattacgtcg tgaacacatt gaagaagctg
     4441 aagtcgatgc attacttgaa tctagaggat tggtttctcg atttgtagga ccttatttca
     4501 aattaatcac gcgtagttgg cacgtattgc cacttggctt tttatttgga cttggttttg
     4561 atacagctag tgaaattgcg ttactcgctc tttcttcagg cgcatcacaa caagccattt
     4621 cgtttatcgg aatcttatct ttaccaattt tattcgcatc aggtatgagt ttattggata
     4681 cattagatgg tgttgtaatg aagtatgcct ataattgggc attcttcaat cctattcgca
     4741 aaatctatta caatataacg attactgcga tatcagtcat ggcagcatta gtgattggga
     4801 tgattgaatt gctacaaatt cttgctgata agctagattt acatggtgcg ttttgggcat
     4861 tcattggttc gatcgaattt gattatttag gctatatttt agttgcatta tttttaatta
     4921 cttggcttat ttcaagttta atttggaagt ttggtcgcat agaacacaaa tggtctaaat
     4981 aaagtaactt gtcttgcaac tacctttgta ttgttgcatt tttagacacc ctgcacactt
     5041 cttccattat gctacgctct cagtagtcat ctttaaatcg tttcttttag agatgacttc
     5101 attttgagag cgtatttttc tatttaaaat gtatttatcc aaaattcaat gctttcgaat
     5161 ttaaaatttc agtatttctg tttaaaattt aattataatc tttaatacag gaggcgctat
     5221 gcttatgaat attgcgaagt tagagaatta tttacaaatt gattcatctc gttataatcg
     5281 accgagtatt gaagcattaa attattatgc aacacgtttt atgttaactg taccttttga
     5341 aaatattgat gttcaaaata gtaagcctat atctataaat atcgatgcac tttttaataa
     5401 aattgttcat gataaacgtg gtggtttttg ttatgagtta aatacatttt tcaaagccta
     5461 tttacaacaa aaaggattta atcctgcatt aatgtcagct actattcaca cacccggtgg
     5521 cggtcgtagt ctgaacggtt cacatgcttc acttgttgtt tctataaatg atgtattcta
     5581 tgtaactgat gttggctttg gagacttacc tatacatgcc attcctatta cttcatctga
     5641 gcatacacaa ccaattactg atatcagtgg tacattccgc gctattttta acaacgaaga
     5701 caaggatatt ttctatgttc aaaaattcga aaatgatcat tggcatacaa aatatgaagc
     5761 tgaattcaaa cctaaacaaa tagaagattt taatagcaac atcgaatata atcaaaccaa
     5821 cccagattca atatttgtac agcatttatt aattacaatg ccacaatcat ttggccgtgc
     5881 tacaatgtct gaaaatcatt taactttaac aaggaatggt agctcagaaa agttagctgt
     5941 cactaaaaat aactataaac attttttaga aaaatatttt ggactagata taactattaa
     6001 tcgtattgaa aaacaataat agtaaaatat aaaaatatgg gcgtatctta cttattgtag
     6061 gtagcgcccc gtttttatgc ttcataaaca caacaaaaag gatatgacac aaacttcatg
     6121 tcacaccctt cttttgaacc gcttcatttt acttaagcaa gtgcatctac aacataaatg
     6181 acacctacga atacaaatgc aatagcaatc atcgtcgaaa taaagacgat acccattaat
     6241 ggattaaata ataaaatgac accgaaaaca atacctaata aattaaaaat cactgaaatt
     6301 agttttaagc taccactacc cgtaaacgta aataatcctg caatagaact aaaaataaac
     6361 cagaatgcaa acatgtagat aaaaaatgcc gagctagcgc caacattaaa aatgacaata
     6421 agaccaaaga gaatatctac aatccccata aacagaatcc aattttggtt accacctact
     6481 aaagcttttg cttttctacg gtaaatgatt tgaatcacac cgttaattaa tacaaacagt
     6541 ccaatcaacc aggtaatagc ataaaaattt tcaattggaa atgtaaaaat aacgactgcc
     6601 aacattaata ataatacacc cattattaaa ctagaccatt taataccttg gtttcctttt
     6661 gccataacaa tcaattcctt tcaaataaag ccctctttta atttttacaa aaaatttatt
     6721 atacaataat taaattccct tttctcaatt tacacaaaca tcatcactta gaaagcacca
     6781 ttgcttttgt ctaatatgta cgatgaatct gtatcctcac tcaaaaatct actacgtagc
     6841 gatagctttc aactctgtaa ggttcaaaca ttgaattgta agtttagata aaggctacta
     6901 ctccaaacat tatttaaaat gaaattaaat acaaaaggag tgagactatg acaatattat
     6961 cagtgcaaca tgtttcaaaa acatacggta aaaagcacac atttcaagca cttaaagata
     7021 ttaactttga catacaaaaa ggcgaattcg ttgcgattat ggggccttct ggatcaggta
     7081 agacaacctt attaaatgta ctaagttcaa ttgaccaaat ttctagcggt agcgtgattg
     7141 ctaacggaca agagcttaat aaacttaatc aaaaagcact tgccaaattc cgtaaagaat
     7201 cattaggttt catcttccaa gattacagta ttctgccgac attaactgtt aaagaaaaca
     7261 ttatgttacc tttatctgtt caaaaaatgt cgaaggcaac aatggaagaa aattacaaag
     7321 cgatcacgac agcattaggt atttatgacc taggaaataa ataccctagc gaattatctg
     7381 gtggtcaaca acaaagaact gcagcagcga gagcatttgt tcacaaacca caaatcatat
     7441 ttgcagatga gccaacaggc gcactcgact cgaaaagtgc aaatgactta ttacaacgtt
     7501 tggaagaaat gaataaatcg tttgatacaa ctattgtcat ggttacacat gatccggttg
     7561 cagcaagttt tgcagaacgt gtcatcatgc taaaagatgg ccaaattcat acacaacttt
     7621 atcaggaagg acgttctaaa caggcctttt atgaagacat tgtacatctt caatcagtat
     7681 taggtggtgt ctcaaatgac atttaaccat atcgttttca aaaatttacg ccaaaactta
     7741 aaacattatg caatgtattt attttcatta ttttttagca tcgtcttata tttcagtttt
     7801 acaaccttac agtttactaa aggtgtaaat aatgacgact cgatggccat cattaagaaa
     7861 ggtgctttag tcggatcaat atttttattt atcattattg tcatcttttt aatgtatgcc
     7921 aatcatttat tcgtgaaacg tcgtacacgt gaatttgcgc tatttcagtt gattggttta
     7981 acacgacaaa acattttgaa aatgttagca cttgaacaaa tgattgtatt tttaatcaca
     8041 ggtgttgtcg gtgtattatg tggcattgca ggtgcacaat tattgctttc catagtatct
     8101 aaattgatgt cgttatcgat taacttatcg atacatttcg aacctatggc ccttgtttta
     8161 actattttca tgctaattat tgcgtatgta ctgattttat ttcaaagtgc tttatttcta
     8221 aaaagacgta gtatcttatc aatgatgaaa gactcaatta aaactgatgt tacaactgcg
     8281 aaagttacag ttatagaagt catttcaggc gtattaggta ttgctatgat tgcactaggc
     8341 tattatatgg cgacagaaat gtttggaaca tttaaagcac taactatggc tatgacatca
     8401 ccgtttatca ttttattttt aacggtagta ggcgcctatt tattcttcag aagttccgtg
     8461 tcactaattt ttaaaacttt gaaaaaatct aaaaatggac gcgtatctat tacagatgtt
     8521 gtatttacat cgtctattat gtaccgaatg aagaaaaatg cgatgtcttt aactatcatt
     8581 gcaattattt ctgcagttac agtaactgta ttgtgtttcg cggcattatc taaatcaaat
     8641 acagatcaaa cccttacatc tatggcacca aatgaattta acgtggtcgc tactcaagat
     8701 gctaaacaat ttgaaactaa actaagccaa caacaaatta cattttctaa aaatgcttac
     8761 gaaactatta ctgtagataa tgttaaagat caggttatta ctttggaaaa tggtagcgat
     8821 tcaggtcgca cgaattctat tttaagtgca aataacaagt taacaggcaa caatgccatc
     8881 attacaaata caaaatcgct gcctaacatt attaatatcc atttaaacaa agatttagta
     8941 gtaaaaggta ctaagaatga aactttccgt gttacacaag aagacaaagg taaggtttat
     9001 cctttaaatc taagcttcaa ctcacctgtc atcgaagtaa gtcctgaaaa gtatcagcag
     9061 ttgaaaacac aaaataacgt tcatactttt tatggatatg atattaaaca aacatcacaa
     9121 aaggaaaaag ctcaaacaat tgcaaaacag tttggagaca aagtcatcac gtatgatgaa
     9181 atgaaaaaag aggtcgatgc tactaacggt attctaatat ttgttacatc atttttagga
     9241 ttagcatttt tagtagcagc tggatgtatt atttatatta aacaaatgga tgaaactgag
     9301 gatgaactaa gtaacttcag aatattaaaa cgcataggct ttacgcatac agatatgctt
     9361 aagggattat tattaaaaat tactttcaac ttcggtttac cattattgat tgctatatta
     9421 catgcagtat tcgccgcaat agcatttatg aaattgatgg gtaatatttc cttcatgccg
     9481 gttattgtag taattgttgt atacacactc atttacatta cttttgcttt aattgctttt
     9541 gtacattcaa acaaattgat taagaaaacc atttaacact tatgattaaa agatgcttca
     9601 ttgagatttt gattttaagc aatgcagtat aactattaac tgatttataa aaaaggagag
     9661 attgaatcat gaaatttaga gaattagtaa aacaatcata cgaggatttg aaaaatttga
     9721 ctgtaacttg gtataacgtc attgctttag caatgattgt tattgtctta agcgtagtta
     9781 caacaccaat tataggtatt ccagctggtt tattaggtgg cgcctattat ttaaaacgtc
     9841 gtgaagaaaa aggcaagtaa gcacattcca tcggtataga acattgctat gacctttcaa
     9901 gtcagatttt aatatcaaca agtagcgttt atcgctgctt gttttttatg gattgcttat
     9961 aaaatcatca tgtttaaacg atatgtcact ttgaaaacta gcaaacacca taaaagacta
    10021 caattttttt atttttataa acccctttaa atcaacgttt ctaaggtttt taaaaatctt
    10081 ttttaattta ttttcaaaaa acactgtaca ttatgccaat atgagcgtat agttggtctt
    10141 acgtaataaa agctcgtgaa ttaaattgta gtgtatttgt ttagaatatc ctctttttta
    10201 gttatgaatt tgttacaaat attaagtgca aaagcacatg gaggttttct atatgaataa
    10261 cggtacagta aaatggttta acgcagaaaa aggttttggt ttcatcgaac aagaaaatgg
    10321 cggagacgta ttcgtacatt tctcaggtat cgctagcgat ggctacaaaa ctttagaaga
    10381 aggtcaaaaa gttactttcg aaatcactga aggtcaacgt ggagaccaag cagttaacgt
    10441 acaaactgtt taatcttaca aaataaaacg actcattata aatgaatcgc tttgattacc
    10501 aacaaggttc taaatcttgt tggtattttt ttatgctatt ttggataata tgagacaata
    10561 cgatgatgat ttataaaaac ttatttgcca aatacacact aaggaaaatt atgaggctga
    10621 gtataccaat tactgatata atagaaagcg catttgtcat cgtatttcta gctttagtcg
    10681 tttgtacttc actaggatct tttccattca taaaagcaac aattcgatcg tagttgtcca
    10741 ttttatgcac tttttttaaa tcttctattt tcatagatgc ataaaaacct attcccaaaa
    10801 tgattgccac cgttacaccc caagtccaat tccaataaac aactaaaggt cccattaaaa
    10861 ttgcagctaa tgtcatgaaa ataagcatca catatgtcca taagttaaga cttcgttgcg
    10921 ctttaatatc aggtacaaat ggaatggtcc cttttactaa atcatctaaa gttacattga
    10981 acaattcgca catcattaat aagttatgta tgtctggtaa gcttttgtca ttttcccaat
    11041 tagaaatact ctgcctagat acatataact tttcagcaag atattcttgt gaataaccat
    11101 ctcgttccct atatttttta atttgtttac tcagattcaa ttacatacgc ctccagtcaa
    11161 ttagtacaat cattacaatc agactatcag ataaacttag ttaaagtcac ctttatgatt
    11221 gactaaatgt tttgacatac gacaacatca gggtttgaag acttcctcaa gacagacctg
    11281 tatgattacg cgcttgtaat tttctcaatg ccaacttgaa tttcatttat taatgatgca
    11341 acttttccat gctattcaat gcttgcgaag ttagtcctat ttaagagatt acgttcacac
    11401 tatttactat tcgattttcc agctttccaa ctatgctttt ctaatctttt ttgtgctata
    11461 aattctactg cttcaaaaat acatactgca ctgatgaacc agcttaggaa accaacaaca
    11521 atgctaatca accacatgtg cgtcatatgt aacatcatcc atgtaactaa aatacaaact
    11581 aatacccctg tcataccaaa cactgcgcca cgacttacat gcacagcgac tttatcacca
    11641 tattgcatac ctgtaataaa catagacact aaaaatactg ccggaaacgt tgcaaatata
    11701 ccgccaaatt ctttccaagg tagtgttaca gatacaatat aacttaataa tactgcaaat
    11761 ccccccacaa aaaatttcat taatgttaat ttcatgatgt tctgcctcca aaaatttatt
    11821 acttaataac tgaatacaac taattatcac gcattttcca aactataaat gtctaatact
    11881 gaatattgca agggaaataa caaaccaaca aacaactgtg aatattgcgc ctttccgata
    11941 acctttatgc ttaatataga gagatgtcaa aaatacagtt aatatacaag acagaatacc
    12001 gacaattgct ccagtactaa gattcatcga catctccact aattgtgtac cacgatgatc
    12061 caaagctaat gcgataatag ccgcaagaaa gactgccggc atagtagcta taatacctcc
    12121 taacttacca cctaccttat cagcaataat tgaagctaat gcaacagcga taccaccaat
    12181 gacaaaatga agtattgcac ttccaattga aaacatcttc aacgtacctt tctataatta
    12241 attcaataca aaacgtatca ttcacacaaa gtgtaaaact taaattttct actgtaaagg
    12301 caactcaagc gatgttcata aagctttcat gatactttca taaactagca ataaagggtt
    12361 caaatgaagg tatatccagc tcaaattatt gaaaattacc attaactgta attttcaaat
    12421 caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaagacaa agctgttatg
    12481 atcttagctt tgtcttttat gtgttagtca tcatgtatac gacatcctac tactaacgtt
    12541 tatatattcg aatttttatt tcataataat cttcatgatc tttgtcttta tgctcaacat
    12601 gtagtcctgt ttgttgaatc gcttggatac ttttgcctac ctcatctcgt gcttgagtga
    12661 catcttgtgc aaagcgaagg ttttgtgctt tgactttttc gggcccagtt ttttggcgta
    12721 ctctatcttc agtctgtttc acatttagtt tttgtgcaat gacttgctca atcaacgctt
    12781 cttgttcact atcagacaat gataataccg ctctcgcatg gcgttcagta attttacctt
    12841 ctcttaagcg aagtagtact ttcggcgcca acttcaataa acgcaactta tttgcaataa
    12901 agctttgact tttacctaaa ctttttgcca attcactttg cgttgtatca ccaatttcca
    12961 ataatttctt ataggcttcc gcttcttcaa caacagacaa attttctctt tgaatattct
    13021 caattaatgc aacaacagcc gtctcttcat catccatatc acgaataata acgtctgctt
    13081 gaggtaaatt tagtgattgt attgctctaa atcggcgctc tccagcaata atttcaaaca
    13141 tatcttcttc aatcggtctt acaacaatag gctgtaataa accatgttca tgtattgatt
    13201 cagcaagttc tttaatttta tttggttcaa acacctgtct tggttgataa cggttgggaa
    13261 cgatacgttc aatttgaatg gattcaacat tactattgcg atcttcttca atatgtccaa
    13321 tgatgtcatc tttgtttttc aaaccaaata attttgaaaa aggttttttc attatccatt
    13381 cgctcctttt atgttagttg taaaggatta tgcattattt ttcaagtaaa ggagtcttat
    13441 taggcgtccc tggttttctt ggatactttt tcggcgtctg tcttttttta tcaataatga
    13501 acatctggcg ctctccagca tcttctggca attcgaaagt atgtgtttct gtaacattac
    13561 cacctaacac actaattgca aattttgctt cttctaattc ttcttcacct tttgaagatt
    13621 ttaatgcaac aaactgtcca ccttttttaa ctagcggtaa acacaattca cttaacacgg
    13681 ataatctagc tactgctctt gcagtaacaa catcataaga ctccctgtag acacccttac
    13741 caaatgtttc tgctctatcg tgtataaagc tgacatcctg taattgtaat tctgacgcta
    13801 aatggtttaa aaattgaata cgcttattta atgaatcaac aatcgtcact tttaactgcg
    13861 gaaacattat ttttaacgga atacttggaa aaccagctcc agcgcctaca tcacatatac
    13921 ttataggctg attaaaatca aaataaaaac taggtgcaat ggaatcataa aaatgtttca
    13981 aatatacatc gtgttcatct gtaatacttg tcaaattcat cttttcattc cattcaacaa
    14041 gtaaacgata atatgtttga aactgttgtt tttgagtctc agttaattga atattatgtt
    14101 cttttaattg ttctgctaac cattctacag tcattagtca ctcacccttt ggagtttacc
    14161 ttgttctaag taaatcaata atatagatat gtctgctgga tttacccctg atattctaga
    14221 agcttgtgca atatttaaag gttttacttc tgataatttt tctcgcgctt cagtcgccaa
    14281 actatcaatc ttactataat ctaagtcttc tggaattttc ttctcttcca tacgcttaac
    14341 tttctcaact tgttgtagtg atttattgat ataaccttca tattttgttt gtatttctac
    14401 ttgttcttca acatctgcat tcaattgatg ttcttcttct aaaatttcta aaattatatc
    14461 gtaagtcatt tcaggtctgc gtaataaatc gatagctaaa ataccatctt ttaagcgaga
    14521 accaccatgt tgttcaataa tcgcttgcgt atgttcgttt ggtttaatac gaatatctga
    14581 taaacgctta atttccgcat caatttgctg acgtttttca ttaaaacgtg catatcgttc
    14641 ttcagaaatc ataccaagtt catatcccat atccgtcaat ctcaaatcag cattatcatg
    14701 acgtagtaac aaacgatatt ctgcacgtga tgttagtaaa cgataaggtt cattagtacc
    14761 tttagttaca agatcatcga ttaagacacc aatatatgca tctgaacgac ttaatatctt
    14821 ttcgcctgtg tttaacactt tacctgcagc gttaatacct gccatcaatc cttgtcctgc
    14881 tgcttcttca taaccagatg taccattaat ttgacctgca gtatataagt ttttaatcat
    14941 tttcgtttca agtgtaggcc ataactgcgt tggcacaatc gcatcatatt caattgcgta
    15001 gccggcacgc atcatatctg ctttttcaag acctggtatc gtctctaaca tttgacgttg
    15061 cacatgttca ggaagacttg tagacaatcc ttgcacatat acttcatttg tattacgacc
    15121 ttcaggctct aagaaaagtt gatgtcgcgg cttatcatta aatcgaacaa atttatcttc
    15181 aattgaaggg caataacgtg gcccggttcc tttaatcatc cctgaataca ttgcagatag
    15241 atgtaaatta tcatcgataa ctttgtgtgt ttcagcatta gtatacgtta gccaacatgg
    15301 caattgatct aatatatatt ctgttgtttc aaagctgaat gcacgaccta catcgtcacc
    15361 tggttgtatt tcagtcttcg aatagtcaat tgtttttgaa tttacacgcg gtggtgtacc
    15421 tgttttaaaa cgaacaatat caaaaccaag ttctcttaaa ttgtctgata atgtgattga
    15481 tggtaattgg tgatttggtc cacttgaata cttcatatta cctaaaatga tttcaccacg
    15541 taaaaatgtt cccgttgtaa taattactgc tttagataaa tactctgtac caatatttgt
    15601 acgtacacct ttaacttcat tatcttctat aataagttcg tctaccatac cttgcattat
    15661 atgcaaattt tcttcatctt caatcacgcg tttcatttct tgttgataaa gtactttatc
    15721 tgcttgcgct cttagtgctc ttacagcagg tcctttacct gtatttaaca ttctcatttg
    15781 aatgtgtgtt ttatcgattg tttttgccat ttgtccacct aaagcatcaa tttcacgaac
    15841 aacgatacct ttagctggtc cacctacaga tgggttacat ggcataaatg caatattatc
    15901 taaatttatt gttagcatta atgttttagc accacgtctt gcagatgcta aacctgcttc
    15961 tacacctgca tgtcccgcac ctataacgat tacatcatat tcttgaacca caatataaac
    16021 ctccttattt gatatcttac tagccttctt aagacggtat tccgtctatt tcaattacta
    16081 tttacctaag cagaattgac tgaataactg atcgatgagt tcatcacttg cagtctcacc
    16141 aataatttct cctaatattt cccaagttct agttaaatca atttgtacca tatccatagg
    16201 cacaccagat tctgctgcat caatcgcatc ttgtatcgtt tgtcttgctt gttttaataa
    16261 tgaaatatgt cttgaattag aaacataagt catatcttga ttttgtactt ctccaccaaa
    16321 gaacaaatct cgaatttgta tttctaattc atcaatacct tcttgtttta acattgaagt
    16381 ttgaattaat ggcgtatcac ctatcatatc tttaacttca ttaatatcta tgttttgctc
    16441 taaatccatt ttattaacaa ttacgattac atcttcattt ttaaccactt catataatgt
    16501 gtaatcttct tgagtcaatg cttcgttatt gtttaataca aataaaatta agtctgcttg
    16561 gctaagagcc tttctagagc gttcaacacc aatcttctct actatatctt ctgtctcgcg
    16621 tataccagca gtatcaacta atcttaatgg cacgccacga acattgacgt actcttctaa
    16681 gacatctctt gtagtacctg ctacctcagt tacaatcgct ttattatctt gtattaaatt
    16741 atttaacatc gatgatttac ctacgtttgg tttaccaaca ataactgtag ataaaccttc
    16801 acgcataatt ttaccctgcg caccggtatc taataaacga ttaatttcct gtttgatttc
    16861 tttagactgc tctaaaagaa attcagtagt cgcatcttca acatcatcgt attcaggata
    16921 atcaatattc acttccactt gagcgagtat ctctaatata gattgacgtt gttttttgat
    16981 taagtcactt agacgacctt caatttgatt catcgcaact ttagaagctc tatctgtttt
    17041 cgagcgaata aagtccataa ctgcttcagc ttgagataaa tcaatacgac cattcaaaaa
    17101 ggcacgtttt gtaaattcac ctggctcagc cattctagcg ccatatgtca tagtaagttc
    17161 cagcactcta ttaatcgtta aaataccacc atgacaatta atttctataa tatcttcgcg
    17221 tgtaaatgtt tttggcgctc ttaacacaga caccataact tcttcaacca cttctttaga
    17281 ctctggatca ataatatgac cgtaattaat cgtatgtgat ggaacatcat ttaaaagatg
    17341 ttttccttta tataatttgt cagcaatttc aacggcttgc ggtccagaca atcgaacaat
    17401 tccaattgcc ccttcaccca ttggtgttga aatactcgta attgtatcta aatccatatt
    17461 gctactcgcc tccttcaacg atgtgaatac attttaaagt aagttattat aaccctaagg
    17521 tcagtcttac gtttgtctga ggtaagactt cgggatgcgt tgagtggtta atgttttcct
    17581 tcccctaccc tatccttact taatcttttt attaaaaact ttggcaattt taagtacgtg
    17641 ctcaagacta ttctgtattt gtaaagtcgt catatcttta gctggctgtc ttgctattac
    17701 aataatatct ttggccaata tatgcgactt atgtactttg aaattttcac gtattgctct
    17761 tttaatcttg tttcttaaca ctgcattacc tagtttttta gaaacactaa tacctaagcg
    17821 aaaatggtct atttctttat tattacaagt gtatacaaca aattgtctgt tggctacaga
    17881 atgacctttt ttatatattc tctgaaaatc tgcattcttt ttaattcggt aagctttttc
    17941 caataacatc actcgcttat ttatcgtttt tatttgaagc tatatttaaa cttctattga
    18001 gcttataaca taaatttcta tttattctta atttaaacga aaaaaaagat cactgatgag
    18061 tcagtgatct tatgcagata aaactttacg gcctttacga cgacggcgcg ctaaaacttt
    18121 acggccattt tttgtgctca tgcgttttct gaaaccatga actttactat gtttacgttt
    18181 atttggttga taagtacgtt ttaccatgca aaacacctcc atctttatac agttatctaa
    18241 tataccagat tatgcagact ctgatcactt taatagataa gattttgcgt ttcttagatt
    18301 aaaattctcg tattagctca ttgattatct agtcataatt caagcaacta ctacaatata
    18361 acaaaatcct ttttataacg caagttcatt ttatactact gctcaatttt tttactttta
    18421 tcgattaaag atagaaatac acgatgcgag caatcaaatt tcataacatc accatgagtt
    18481 tggtccgaag catgagtgtt tacaatgttt gaatacctta tacagttctt atacatactt
    18541 tataaattat ttcccaagct gttttgatac actcactaac agatattcta tagaaggaaa
    18601 agttatccac ttatgcacat ttatagtttt cagaattgtg gataattaga aattacacac
    18661 aaagttatac tatttttagc aacatattca caggtatttg acatatagag aactgaaaaa
    18721 gtataattgt gtggataagt cgtccgactc atgattttat aaggatttat ttattgatat
    18781 ttacataaaa atactgtgca taactaataa gcaggataaa gttatccacc gattgttatt
    18841 aacttgtgga taattattaa catggtgtgt ttagaagtta tccacggctg ttatttttgt
    18901 gtataactta aaaatttaag aaagatggag taaatttatg tcggaaaaag aaatttggga
    18961 aaaagtgctt gaaattgctc aagaaaaatt atcagctgta agttactcaa ctttcctaaa
    19021 agatactgag ctttacacga ttaaagatgg tgaagctatc gtattatcga gtattccttt
    19081 taatgcaaat tggttaaatc aacaatatgc tgaaattatc caagcaatct tatttgatgt
    19141 tgtaggctat gaagttaaac ctcactttat tactactgaa gaattagcaa attatagtaa
    19201 taatgaaact gctactccaa aagaaacaac aaaaccttct actgaaacaa ctgaggataa
    19261 tcatgtgctt ggtagagagc aattcaatgc ccataacaca tttgacactt ttgtaatcgg
    19321 acctggtaac cgctttccac atgcagcaag tttagctgta gccgaagcac cagccaaagc
    19381 gtacaatcca ttatttatct atggaggggt tggtttagga aaaacccatt taatgcatgc
    19441 cattggtcat catgttttag ataataatcc agatgccaaa gtgatttaca catcaagtga
    19501 aaaattcaca aatgaattta ttaaatcaat tcgtgataac gaaggtgaag ctttcagaga
    19561 aagatatcgt aatatcgacg tcttattaat cgatgatatt cagttcatac aaaacaaggt
    19621 acaaacacaa gaagaatttt tctatacttt taatgaattg catcagaata acaagcaaat
    19681 agttatttcg agtgatcgac caccaaagga aattgcacaa ttagaagacc gattacgttc
    19741 acgctttgaa tgggggctaa ttgttgatat tacgccacca gattatgaaa ctcgaatggc
    19801 aattttgcag aagaaaattg aagaagaaaa attagatatt ccaccagaag ctttaaatta
    19861 tatagcaaat caaattcaat ctaatattcg tgaattagaa ggtgcattaa cacgtttact
    19921 tgcatattca caattattag gaaaaccaat tacaactgaa ttaactgctg aagctttaaa
    19981 agatatcatt caagcaccaa aatctaaaaa gattaccatc caagatattc aaaaaattgt
    20041 aggccagtac tataatgtta gaattgaaga tttcagtgca aaaaaacgta caaagtcaat
    20101 tgcatatccg cgtcaaatag ctatgtactt gtctagagag cttacagatt tctcattacc
    20161 taaaattggt gaagaatttg gtgggcgtga tcatacgacc gtcattcatg ctcatgaaaa
    20221 aatatctaaa gatttaaaag aagatcctat ttttaaacaa gaagtagaga atcttgaaaa
    20281 agaaataaga aatgtataag taggaaactt tgggaaatgt aatctgttat ataacagcac
    20341 taatgataac aatcattttt tacatttcta tatgctaatg tggcaagatg agcaaaactc
    20401 attttgtgga taatgtttaa aagtcataca caccatacac aagttatcaa catgtgtata
    20461 acttcgccaa atctatgttt ttaagactta tccaccaatc cacagcacct actactatta
    20521 ctaagaactt aaaacctata taattatata taaacgactg gaaggagttt taattaatga
    20581 tggaattcac tattaaaaga gattatttta ttacacaatt aaatgacaca ttaaaagcta
    20641 tttcaccaag aacaacatta cctatattaa ctggtatcaa aatcgatgcg aaagaacatg
    20701 aagttatatt aactggttca gactctgaaa tttcaataga aatcactatt cctaaaactg
    20761 tagatggcga agatattgtc aatatttcag aaacaggctc agtagtactt cctggacgat
    20821 tctttgttga tattataaaa aaattacctg gtaaagatgt taaattatct acaaatgaac
    20881 aattccagac attaattaca tcaggtcatt ctgaatttaa tttaagtggc ttagatccag
    20941 atcaatatcc tttattacct caagtttcta gagatgacgc aattcaattg tcggtaaaag
    21001 tgcttaaaaa cgtgattgca caaacaaatt ttgcagtgtc cacctcagaa acacgcccag
    21061 tactaactgg tgtgaactgg cttatacaag aaaatgaatt aatatgcaca gcgactgact
    21121 cacaccgctt ggctgtaaga aagttgcagt tagaagatgt ttctgaaaac aaaaatgtca
    21181 tcattccagg taaggcttta gctgaattaa ataaaattat gtctgacaat gaagaagaca
    21241 ttgatatctt ctttgcttca aaccaagttt tatttaaagt tggaaatgtg aactttattt
    21301 ctcgattatt agaaggacat tatcctgata caacacgttt attccctgaa aactatgaaa
    21361 ttaaattaag tatagacaat ggggagtttt atcatgcgat tgatcgtgcc tctttattag
    21421 cacgtgaagg tggtaacaac gttattaaat taagtacagg tgatgacgtt gttgaattat
    21481 cttctacatc accagaaatt ggtactgtaa aagaagaagt tgatgcaaac gatgttgaag
    21541 gtggtagcct gaaaatttca ttcaactcta aatatatgat ggatgcttta aaagcaatcg
    21601 ataatgatga ggttgaagtt gaattcttcg gtacaatgaa accatttatt ctaaaaccaa
    21661 aaggtgacga ctcggtaacg caattaattt taccaatcag aacttactaa aaataaatat
    21721 aaataaagga tgacgtgatt aattaaaacg tcatccttta ttttttggca aaaataattc
    21781 tagatgcgta tgtaaaataa atttggcagc attttaaaca gcaaataaaa gacgccaatt
    21841 aaatttatga caaatatatc caaaatttaa taagtgtgct tatatgccct ttaaatttaa
    21901 aattttaata gtcaataaca agttgaatat aaaagttaaa cgccgttaaa tagcgttaaa
    21961 aaattgaaaa tgacagtatt gccaaaaaat aagaattaat tatttatatg taaacggttt
    22021 ctacctctat tttaaatgaa atttgtgaca aaaaaaggta taatatatta atgacataca
    22081 aagaaatgga gtgattattt tggttcaaga agttgtagta gaaggagaca ttaatttagg
    22141 tcaatttcta aaaacagaag ggattattga atctggtggt caagcaaaat ggttcttgca
    22201 agacgttgaa gtattaatta atggagtgcg tgaaacacgt cgcggtaaaa agttagaaca
    22261 tcaagatcgt atagatatcc cagaattacc tgaagatgct ggttctttct taatcattca
    22321 tcaaggtgaa caatgaagtt aaatacactc caattagaaa attatcgtaa ctatgatgag
    22381 gttacgttga aatgtcatcc tgacgtgaat atcctcattg gagaaaatgc acaaggaaag
    22441 acaaatttac ttgaatcaat ttatacctta gctttagcaa aaagtcatag aacgagtaat
    22501 gataaggaac tcatacgttt taatgctgat tatgctaaaa tagaaggtga gcttagttat
    22561 agacacggca cgatgccatt aacaatgttt ataactaaaa aaggtaaaca agtcaaagtg
    22621 aatcacttag agcaaagtcg tctaactcaa tatattggac acctcaatgt ggttctattt
    22681 gcgccagaag atttgaatat tgtaaaaggc tctcctcaaa taagacgacg ctttatagat
    22741 atggagttgg gccaaatttc tgctgtttac ttaaatgatt tagctcaata ccaacgtatt
    22801 ttaaagcaaa agaataatta cttaaagcag ttacaattag gccaaaaaaa ggacttaaca
    22861 atgttggaag tattaaatca gcagtttgct gaatatgcaa tgaaagtaac tgataaacgt
    22921 gcacatttta ttcaagagct agagtcgtta gctaaaccga ttcatgctgg tatcacaaat
    22981 gataaagaag cgttgtcgct gaattattta cctagtctta aatttgatta tgctcaaaat
    23041 gaagcggcac gacttgaaga aattatgtct attcttagcg ataatatgca aagagaaaaa
    23101 gaacgaggca ttagcttatt cggaccacat cgagatgata taagttttga tgtgaatggc
    23161 atggatgctc aaacatatgg ttctcaagga cagcaacgta caacggcttt gtccattaaa
    23221 ttagctgaaa ttgagttaat gaatatcgaa gttggggaat atcccatctt attattagac
    23281 gatgtactca gtgaattaga tgattcgcgt caaacgcatt tattaagtac gattcagcat
    23341 aaagtacaaa catttgtcac tacgacatct gtagatggta ttgatcatga aatcatgaat
    23401 aacgctaaat tgtatcgtat taatcaaggt gaaattataa agtaacagaa agcgatggtg
    23461 actgcattgt cagatgtaaa caacacggat aattatggtg ctgggcaaat acaagtatta
    23521 gaaggtttag aagcagtacg taaaagacca ggtatgtata taggatcgac ttcagagaga
    23581 ggtttgcacc atttagtgtg ggaaattgtc gataatagta tcgatgaagc attagctggt
    23641 tatgcaaatc aaattgaagt tgttattgaa aaagataact ggattaaagt aacggataac
    23701 ggacgtggta tcccagttga tattcaagaa aaaatgggac gtccagctgt cgaagttatt
    23761 ttaactgttt tacatgctgg tggtaaattt ggcggtggcg gatacaaagt atctggtggt
    23821 ttacatggtg ttggttcatc agttgtaaac gcattgtcac aagacttaga agtatatgta
    23881 cacagaaatg agactatata tcatcaagca tataaaaaag gtgtacctca atttgactta
    23941 aaagaagttg gcacaactga taagacaggt actgtcattc gttttaaagc agatggagaa
    24001 atcttcacag agacaactgt atacaactat gaaacattac agcagcgtat tagagagctt
    24061 gctttcttaa acaaaggaat tcaaatcaca ttaagagatg aacgtgatga agaaaacgtt
    24121 agagaagact cctatcacta tgagggcggt attaaatcgt acgttgagtt attgaacgaa
    24181 aataaagaac ctattcatga tgagccaatt tatattcatc aatctaaaga tgatattgaa
    24241 gtagaaattg cgattcaata taactcagga tatgccacaa atcttttaac ttacgcaaat
    24301 aacattcata cgtatgaagg tggtacgcat gaagacggat tcaaacgtgc attaacgcgt
    24361 gtcttaaata gttatggttt aagtagcaag attatgaaag aagaaaaaga tagactttct
    24421 ggtgaagata cacgtgaagg tatgacagca attatatcta tcaaacatgg tgatcctcaa
    24481 ttcgaaggtc aaacgaagac aaaattaggt aattctgaag tgcgtcaagt tgtagataaa
    24541 ttattctcag agcactttga acgattttta tatgaaaatc cacaagtcgc acgtacagtg
    24601 gttgaaaaag gtattatggc ggcacgtgca cgtgttgctg cgaaaaaagc gcgtgaagta
    24661 acacgtcgta aatcagcgtt agatgtagca agccttccag gtaaattagc cgattgctct
    24721 agtaaaagtc ctgaagaatg tgagattttc ttagtcgaag gggactctgc cggggggtct
    24781 acaaaatctg gtcgtgactc tagaacgcag gcgattttac cattacgagg taagatatta
    24841 aatgttgaaa aagcacgatt agatagaatt ttgaataaca atgaaattcg tcaaatgatc
    24901 acagcatttg gtacaggaat cggtggcgac tttgatctag cgaaagcaag atatcacaaa
    24961 atcgtcatta tgactgatgc cgatgtggat ggagcgcata ttagaacatt gttattaaca
    25021 ttcttctatc gatttatgag accgttaatt gaagcaggct atgtgtatat tgcacagcca
    25081 ccgttgtata aactgacaca aggtaaacaa aagtattatg tatacaatga tagggaactt
    25141 gataaactta aatctgaatt gaatccaaca ccaaaatggt ctattgcacg atacaaaggt
    25201 cttggagaaa tgaatgcaga tcaattatgg gaaacaacaa tgaaccctga gcaccgcgct
    25261 cttttacaag taaaacttga agatgcgatt gaagcggacc aaacatttga aatgttaatg
    25321 ggtgatgttg tagaaaaccg tagacaattt atagaagata atgcagttta tgcaaactta
    25381 gacttctaag cgctgtgaac tgaacttttg aaggaggaac tcttgatggc tgaattacct
    25441 caatcaagaa taaatgaacg aaatattacc agtgaaatgc gtgaatcatt tttagattat
    25501 gcgatgagtg ttatcgttgc tcgtgcattg ccagatgttc gtgacggttt aaaaccagta
    25561 catcgtcgta tactatatgg attaaatgaa caaggtatga caccggataa atcatataaa
    25621 aaatcagcac gtatcgttgg tgacgtaatg ggtaaatatc accctcatgg tgacttatct
    25681 atttatgaag caatggtacg tatggctcaa gatttcagtt atcgttatcc gcttgttgat
    25741 ggccaaggta actttggttc aatggatgga gatggcgcag cagcaatgcg ttatactgaa
    25801 gcgcgtatga ctaaaatcac acttgaactg ttacgtgata ttaataaaga tacaatagat
    25861 tttatcgata actatgatgg taatgaaaga gagccgtcag tcttacctgc tcgattccct
    25921 aacttgttag ccaatggtgc atcaggtatc gcggtaggta tggcaacgaa tattccacca
    25981 cataacttaa cagaattaat caatggtgta cttagcttaa gtaagaaccc tgatatttca
    26041 attgctgagt taatggagga tattgaaggt cctgatttcc ctactgctgg acttatttta
    26101 ggtaagagtg gtattagacg tgcatatgaa acaggtcgtg gttcaattca aatgcgttct
    26161 cgtgcagtta ttgaagaacg tggaggcgga cgtcaacgta ttgttgtcac tgaaattcct
    26221 ttccaagtga ataaggctcg tatgattgaa aaaattgcag aactcgttcg tgacaagaaa
    26281 attgacggta tcactgattt acgtgatgaa acaagtttac gtactggtgt gcgtgtcgtt
    26341 attgatgtgc gtaaggatgc aaatgctagt gtcattttaa ataacttata caaacaaacg
    26401 ccacttcaaa catcatttgg tgtgaatatg attgcacttg taaatggtag accgaagctt
    26461 attaatttaa aagaagcgtt ggtacattat ttagagcatc aaaagacagt tgttagaaga
    26521 cgtacgcaat acaacttacg taaagctaaa gatcgtgccc acattttaga aggattacgt
    26581 atcgcacttg accatatcga tgaaattatt tcaacgattc gtgagtcaga tacagataaa
    26641 gttgcaatgg aaagcttgca acaacgcttc aaactttctg aaaaacaagc tcaagctatt
    26701 ttagacatgc gtttaagacg tctaacaggt ttagagagag acaaaattga agctgaatat
    26761 aatgagttat taaattatat tagtgaatta gaagcaatct tagctgatga agaagtgtta
    26821 ttacagttag ttagagatga attgactgaa attagagatc gtttcggtga tgatcgtcgt
    26881 acagaaattc aattaggtgg atttgaagac ttagaggacg aagacttaat tccagaagaa
    26941 caaatagtaa ttacactaag ccataataac tacattaaac gtttgccggt atctacatat
    27001 cgtgctcaaa accgtggtgg tcgtggtgtt caaggtatga atacattgga agaagatttt
    27061 gtcagtcaat tggtaacttt aagtacacat gaccatgtat tgttctttac taacaaaggt
    27121 cgtgtataca aacttaaagg ttatgaagtg cctgagttat caagacagtc taaaggtatt
    27181 cctgtagtga atgctattga acttgaaaat gatgaagtca ttagtacaat gattgctgtt
    27241 aaagaccttg aaagtgaaga caacttctta gtgtttgcaa ctaaacgtgg tgtcgttaaa
    27301 cgttcagcat taagtaactt ctcaagaata aatagaaatg gtaagattgc gatttcgttc
    27361 agagaagatg atgagttaat tgcagttcgc ttaacaagtg gtcaagaaga tatcttgatt
    27421 ggtacatcac atgcatcatt aattcgattc cctgaatcaa cattacgtcc tttaggccgt
    27481 acagcaacgg gtgtgaaagg tattacactt cgtgaaggtg acgaagttgt agggcttgat
    27541 gtagctcatg caaacagtgt tgatgaagta ttagtagtta ctgaaaatgg ttatggtaaa
    27601 cgtacgccag ttaatgacta tcgtttatca aatcgtggtg gtaaaggtat taaaacagct
    27661 acgattactg agcgtaatgg taatgttgta tgtatcacta cagtaactgg tgaagaagat
    27721 ttaatgattg ttactaatgc aggtgtcatt attcgactag atgttgcaga tatttctcaa
    27781 aatggtcgtg cagcacaagg tgttcgctta attcgcttag gtgatgatca atttgtttca
    27841 acggttgcta aagtaaaaga agatgcagaa gatgaaacga atgaagatga gcaatctact
    27901 tcaactgtat ctgaagatgg tactgaacaa caacgtgaag cggttgtaaa tgatgaaaca
    27961 ccaggaaatg caattcatac tgaagtgatt gattcagaag aaaatgatga agatggacgt
    28021 attgaagtaa gacaagattt catggatcgt gttgaagaag atatacaaca atcatcagat
    28081 gaagaataat aaaaaataag acttccctat atgtagggga gtcttatttt tatgctagaa
    28141 agtaatgctt tactatattc aatgattagt aatgactaac tttctaattg tttcattgcg
    28201 taaggtattt cattgataag tcttgatggt ggcaccacat acatatcttt tgcaaggttt
    28261 tcgccaataa aactatgtgt atatgtggca ctcataaccg cttcttttaa gttatcaaat
    28321 tgaccgacaa agcttgtaat cataccagca agtgtatcgc ccataccacc agtcgccatt
    28381 gctgggctac cgattgtcaa tttaaagtct tcatctttaa agaaaatttc agtaccatgt
    28441 tttttaagta caacagttgc acctaaacga tcaactgctt cacgattacg ctcatatgtc
    28501 tgttcctcaa taggaatacc acttaatcgt tcccattctt tgaggtgtgg tgtaaagatc
    28561 acacgacatg taggtaattg cggtttcagt ttactaaaga ttgtaatcgc atcgccgtct
    28621 acgattaaat tttgatgcgg ttgtatattt tgtagtagga atgtaatggc attatttcct
    28681 ttgaaatcaa cgccaagacc tggaccaatt agtatactgt cagtcatttc aatcattttc
    28741 gtcaacattt tcgtatcatt aatatcaata accatcgctt ctgggcaacg agaatgtaat
    28801 gctgaatgat ttgttggatg tgtagctaca gtgattaaac cactaccgct aaatacacat
    28861 gcacgagccg ctaacataat ggcaccacct aagttagcag atccaccaat taataaaatt
    28921 ttgccataat cacctttatg tgaatcttct ttacgcttag gaatgttaat agaatttaac
    28981 gtttccatag tgatataacc tcccatgtaa aagccttttc cgaatttatt caattttaaa
    29041 aatatatagt aacttttaac aaaatgtatt ataaatttct gaattcatta ttatttgtc
//
LOCUS       DAMZLE010000018        28258 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMZLE010000018 DAMZLE010000000
VERSION     DAMZLE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.018"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(304..1329)
                     /locus_tag="POB92_000770"
     CDS             complement(304..1329)
                     /locus_tag="POB92_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDI6578290.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(1503..1862)
                     /locus_tag="POB92_000771"
     CDS             complement(1503..1862)
                     /locus_tag="POB92_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDI6578291.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(2079..3110)
                     /locus_tag="POB92_000772"
     CDS             complement(2079..3110)
                     /locus_tag="POB92_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDI6578292.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(3123..3884)
                     /locus_tag="POB92_000773"
     CDS             complement(3123..3884)
                     /locus_tag="POB92_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6578293.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(3881..4855)
                     /locus_tag="POB92_000774"
     CDS             complement(3881..4855)
                     /locus_tag="POB92_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6578294.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(4869..5609)
                     /locus_tag="POB92_000775"
     CDS             complement(4869..5609)
                     /locus_tag="POB92_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578295.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFRQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKVRNEIMETFALNHHQVEPEYYL"
     gene            complement(5951..6463)
                     /locus_tag="POB92_000776"
     CDS             complement(5951..6463)
                     /locus_tag="POB92_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDI6578296.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            6641..6844
                     /locus_tag="POB92_000777"
     CDS             6641..6844
                     /locus_tag="POB92_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578297.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(7196..8155)
                     /locus_tag="POB92_000778"
     CDS             complement(7196..8155)
                     /locus_tag="POB92_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI6578298.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(8801..10288)
                     /locus_tag="POB92_000779"
     CDS             complement(8801..10288)
                     /locus_tag="POB92_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI6578299.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQDAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            10715..11098
                     /locus_tag="POB92_000780"
     CDS             10715..11098
                     /locus_tag="POB92_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDI6578300.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            11105..11431
                     /gene="isdI"
                     /locus_tag="POB92_000781"
     CDS             11105..11431
                     /gene="isdI"
                     /locus_tag="POB92_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDI6578301.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(11496..12641)
                     /gene="cap8P"
                     /locus_tag="POB92_000782"
     CDS             complement(11496..12641)
                     /gene="cap8P"
                     /locus_tag="POB92_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDI6578302.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLADLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(12718..13980)
                     /gene="cap8O"
                     /locus_tag="POB92_000783"
     CDS             complement(12718..13980)
                     /gene="cap8O"
                     /locus_tag="POB92_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDI6578303.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEIYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLKKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(14034..14921)
                     /gene="capN"
                     /locus_tag="POB92_000784"
     CDS             complement(14034..14921)
                     /gene="capN"
                     /locus_tag="POB92_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDI6578304.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKSEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSVLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(14921..15478)
                     /gene="cap8M"
                     /locus_tag="POB92_000785"
     CDS             complement(14921..15478)
                     /gene="cap8M"
                     /locus_tag="POB92_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDI6578305.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKVNVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(15489..16694)
                     /gene="cap8L"
                     /locus_tag="POB92_000786"
     CDS             complement(15489..16694)
                     /gene="cap8L"
                     /locus_tag="POB92_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDI6578306.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGICIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(16728..17966)
                     /gene="cap8K"
                     /locus_tag="POB92_000787"
     CDS             complement(16728..17966)
                     /gene="cap8K"
                     /locus_tag="POB92_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HDI6578307.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISACL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEH
                     GNFRNLLLKFSSILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTIVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            complement(17975..18532)
                     /gene="cap8J"
                     /locus_tag="POB92_000788"
     CDS             complement(17975..18532)
                     /gene="cap8J"
                     /locus_tag="POB92_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HDI6578308.1"
                     /translation="MIVKTFMKSKIFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINTSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKIIKKI"
     gene            complement(18529..19923)
                     /gene="cap8I"
                     /locus_tag="POB92_000789"
     CDS             complement(18529..19923)
                     /gene="cap8I"
                     /locus_tag="POB92_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HDI6578309.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQAYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVVLPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSILFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFLDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGIE"
     gene            complement(19916..20995)
                     /gene="cap8H"
                     /locus_tag="POB92_000790"
     CDS             complement(19916..20995)
                     /gene="cap8H"
                     /locus_tag="POB92_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HDI6578310.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEH
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENRYDFVIVWGSYTGHLFK
                     SFLEKHYKNKFILNIRDYFFENNKLIKYRMKKIVDASRVTTLSSEGFLKFLPKSEKYR
                     IIYSYNMSIIRESNVTDGFKKRWPINIGFIGNVRFNEINQKLIKELANDSRFHMQYFG
                     TGSEKLEVFARENFINNITFSGGFDLKETPKYLNEIDILNNLFGNQNIALDTALSIRM
                     YYALFLNKPIITTDDTFTATEANKFGLGFSINPENLKGIGDELMDWYNNLDVMDINHK
                     REAYRNDVIENNKQFYQEIGRIFNE"
     gene            complement(20998..22122)
                     /gene="cap8G"
                     /locus_tag="POB92_000791"
     CDS             complement(20998..22122)
                     /gene="cap8G"
                     /locus_tag="POB92_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDI6578311.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKKLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            complement(22126..23235)
                     /gene="cap8F"
                     /locus_tag="POB92_000792"
     CDS             complement(22126..23235)
                     /gene="cap8F"
                     /locus_tag="POB92_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDI6578312.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            complement(23248..24276)
                     /gene="cap8E"
                     /locus_tag="POB92_000793"
     CDS             complement(23248..24276)
                     /gene="cap8E"
                     /locus_tag="POB92_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDI6578313.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            complement(24266..26089)
                     /gene="cap8D"
                     /locus_tag="POB92_000794"
     CDS             complement(24266..26089)
                     /gene="cap8D"
                     /locus_tag="POB92_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HDI6578314.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEENPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            complement(26109..26873)
                     /gene="cap8C"
                     /locus_tag="POB92_000795"
     CDS             complement(26109..26873)
                     /gene="cap8C"
                     /locus_tag="POB92_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDI6578315.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGRKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            complement(26876..27562)
                     /gene="cap8B"
                     /locus_tag="POB92_000796"
     CDS             complement(26876..27562)
                     /gene="cap8B"
                     /locus_tag="POB92_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDI6578316.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIASERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            complement(27578..28246)
                     /gene="capA"
                     /locus_tag="POB92_000797"
     CDS             complement(27578..28246)
                     /gene="capA"
                     /locus_tag="POB92_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDI6578317.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
BASE COUNT         9014 a         5317 c         4002 g         9925 t
ORIGIN      
        1 tttttagtta tacaaagtta tttaacaaac aaatgcatcg attttacaat gcatcttgca
       61 tataatgcct caaaaaaatt agcactatat gactatatat tattccaaat gaatgaaatt
      121 tatcaataat tgcgtaagtg aacacatatg tgcgtaaatg ttaaatcatt gtcctaacat
      181 agccgtgact atacttagat gtaatcataa aaaaactact gccatcttta caacagcagt
      241 agtgcttata tgattcattt atataaaatt gaaagcgtta atcgctagcc cagcatcctt
      301 attctattta gctgtataac ttttactagc aatacgacca cttgcaacaa taatatcttt
      361 atcttggaaa ggttcatgat tgaagaaacg ctctaaaata tctttaactc catcttcaat
      421 acgtttttgt gcttctaaag tcatacctga atagtgaacc gtcatagcat ttctaggcat
      481 tgttctccat ggatgatcag caggtgcagg ttgtggatac caaacatcac cagcatatcc
      541 ttgtaaatgc tcggatgcta acgcttcaac taacgcatcg cgatttacaa ttttaccacg
      601 tgcagtattc actaaataac tgtgtttttt catacgactt aaaacatctt tatcaaataa
      661 gttatcagtt tctggtgtta atggtgcatg aattgtaatc gcatcacttg ttgaaacaag
      721 ttcatcaaag cttacaaatt tagacaattt atggtcttgt tgattgattg gatcatagtg
      781 ttgtaatgtt acattaaatg gcgctaatct ttcagcaaca agttgtccaa ttcgaccaaa
      841 tccaaaaata ccaattgttt tgtgttgtaa ttcatgcgca tgattaccta cttgagacaa
      901 gttccattca ccttctactg attgacgatg accttcttca tagtttctaa gaagtattaa
      961 taaatccata accgcatgtt ctgccacact aactgtatta cttcctgtaa cttcaacgac
     1021 accaatattg tgttcacttg ctgccgctaa atctacatgg tcagatccta cacctgctgt
     1081 aattgctaat ttcaagttcg gtgctttttc aatacgttca cgagtcatat atgcaggata
     1141 aaatggcgca ctaataatca catccatatc tggtaaatgt ttatctaagt cttcaccatt
     1201 atctgctaat ataatgaact catgtcctct ttcctctaaa aatgttttta atcctaatgc
     1261 ttttttagta ttaagtaatt gattttcttg accttctact gcttctggaa ataatgctac
     1321 gattttcata tccatctctc ctaaatgata aattttgact tccttaaagt acgtgttgca
     1381 tgttgcttgt tttaaaaata tgacaaaaaa aacggcaccg tttgacatac cacccccttg
     1441 aattattgtt aaattcaaag cgaagttagc aaacagcacc gtgtacctta caccagcaac
     1501 acttattcta ttgacttatc gaaaacaaat attgaaatga cttcatcatc attcaaatca
     1561 atatctgtaa ataattttac aaacttagcg cctacaagac tttccatctc atttacatct
     1621 atttctttat acatctgttt aatcttctct gtgcgtgtat aatggagcat cgattcattg
     1681 cgtttgtcgt ttaggtaaaa actctcaact ttactcaaaa cacctgtcat atgtgcaatc
     1741 gcccaattat ctttaaacga cactcgaata ctattgggtc ctttacccac gtattcttta
     1801 cgataagatc ttactagatt cgagaattgt tgttctttag tatgcgtatc tatagtattc
     1861 accttcttta aaataagaag ttcattaatt gttcacattt tcattataca ccttattaac
     1921 actttttcaa tgataacgat tattattatt acgaaatgta tattttccta gttaaaagga
     1981 tataacaaat taacagaaaa tgtttataaa ctaagattta tattatttta aaaaatgttt
     2041 caacaaacgc atctaaacaa aaaatcagct tatcacactt accccttcaa ctctgcttca
     2101 agtttaccta aatgtcttaa tgttggtgtc aatgctgcaa agaagaatcc ttcttttaac
     2161 ttgcgaactt gtggcgaata tggtgtgtac gctcttgaac caccattgac aacagaagct
     2221 tgatttacat ctagcaataa atagccgatg tccttcttca atgatattaa ttcatttaaa
     2281 tgtgaagtta agttaccgtc atctaatatt gcataatatt cttctctaag ttgacgataa
     2341 cgttttttaa aagcttcaac atcatactct aaatattgat ttattccgtt ttgcgcattt
     2401 gaaaatgcat caattaactc taaagaactt ttaattgagc ctaatcctat tggaatttga
     2461 taagcaataa attgtgggcg aatagttgcc gcaaactgct tcgcatcatg cgtgataatt
     2521 tgtgattgtg gcactacgac ttgattcaaa gtaatttgat acgtagccga gccatttacc
     2581 cctaaaaaat ttgttttttc aacgagagtg ataccatctt gattggcacg tagaatgaac
     2641 atgacaaatt catctgatga ttcatgtttc gaaatcgcac caaaataatg gtcttcttga
     2701 atattactta cagctggcat acgtccactg acaaccaatt gtccatcaac ataagtgtgt
     2761 tcaaggttca acttttctaa atcattaaat gacttcatcg gattagacaa tccggtagca
     2821 cctaatattt ctccagataa caattgctgt tgtaagtcat tatttaaatg tggctgcgtg
     2881 gcattttcta aatacgttga aaaagctaat tggcaccata aacaaaatcc tgttgtcaaa
     2941 caagactgcg atacagcttc gattacttca gcattttttc tcaatgccgc ctcaccgaaa
     3001 taaccatcta caaataattg ttgaataaat gttctcggat aatacgtccc ttcatcaact
     3061 tctactaaat gaggatctaa ttgttctttg ataagcgttt ctaaagtcat catcatctct
     3121 ccttattcac caaatcttct aagtataaac tgctcaatat aactaatgaa tcgatcaata
     3181 ataaaaccaa ataatccgat aaagaatatt gctgctaaaa catcttctaa gttcaacata
     3241 tttcgtgcat caacgattaa aaaacctaat cccgattgtg caccaatcat ttcaccagaa
     3301 actaaaaata tccaacttgt tcctaccgcc atatgtatcc cagccatgat ttgtttaaat
     3361 gccccgggaa ataatatatt gcgatacaat gaccacccag ttaaatttaa atttgccgct
     3421 atttttaaat attgaggttc aatgtctcta acgcctttaa tagtattgaa cacaattggg
     3481 aaaaaagcgg cgataaaaat aatcgcaatc gctggcaaac taccaatacc aaaccataga
     3541 acaacaaatg gtgcccatgc tatcggagat atcggtctaa tcaattgaaa tagcggttcg
     3601 atagcgttgt atagccaacg attccttcca agcaagaagc ccaatggaat agcaaccaac
     3661 aatgcgacaa caaagcccgc tacaaatctc cataaactaa ttgctaaatg ttggaaaatt
     3721 tctccagtaa caatgaaagt ccatatactt tttcctacaa gagcaggacc aggtaacaat
     3781 acaggttggt aatgcccaat aataatgacc atttcccaaa tgcctaagaa aataataaat
     3841 gtgataatag gtaatataaa tttgttattt gtgggacgtg tcatgaacgc gatgcctcct
     3901 tatacaatga cggttcaaca aagtcatcat atgcaggtgg attaaacaaa tgatgttgtt
     3961 ttaccaatgt cgtaatttct tgatagccgg atggcttaat tgttaaatca ccataggatg
     4021 tccatgccgc tgactgtgtt aaaacgtcac gactttgttt aaaatgatgc gtcataatgt
     4081 ctacactttg cttgcgatca ttcattttaa agccagactt tttataatct tgtacaaatg
     4141 cttgcgctac atccttgtgt tgatcaagca attcccctct cagtactagc acacagcaat
     4201 acgcatcagg tataacgtca tcaccatgtt tcaaagtctt acctttgcct aacttttcac
     4261 ccagtgcacc gaatggttcg gctacagaat accctgtaat tctgtgttca ctcaatgcgg
     4321 ctggcatttc tgctggcgac atttcatgat agctaaaatg ccccggttta atctttaatt
     4381 gtttacgtaa ttcctcaagt aaaagataat gtgttgaata acgatgtggt ataccaaaat
     4441 ggtaatcatc gccattatta ttaaattcat ttaagtgcat acctttttgt cccataatga
     4501 cattgccttc atgatggccc aatgccacag cctttatatt tgagcccttc tgttttgatt
     4561 tcatcgctag ctctattaaa gttgatgcac catcaatacg accactgttt aatgcgtcca
     4621 ttaaatctgg ccaattattg aatttaacta attctagttt atatttcgga tgattgtatt
     4681 gtgataataa ttttttagtc atcatcaaat tagctgaatg tgtaatcggc aaatatccaa
     4741 ttttaatcac ttgctgattt tgggcatttt tagacggttc tttagacgtc ctttgccaat
     4801 cacatcctgt aattataaag attccaatga tgacgattat gcttaacctt ttcatcgtca
     4861 ctcactcctt ataaataata ttcaggttca acttgatgat gattcaatgc aaatgtttcc
     4921 ataatttcat tacgaacctt aagtaggtgg ctatcattac gactgcgtgg atgtgatgct
     4981 gtaatttcat attgagaaat aatattgcac ccttcaccta acagaacaat gcggtcggaa
     5041 agataaatag cttcatcaat gtcatgcgtc actaaaataa tagttgattg cgttttatgt
     5101 tttagttgca ctagttgatc ctgaagttta taacgtgtaa atgcatctaa tgcacctaat
     5161 ggctcatcca tcaatataac gttaggctta tgcacatgcg ctcgacatag tgccacacgt
     5221 tgtttcatac ccccggacag ttgctcggga aaatgctttc ccctgccttc taaatcaact
     5281 aatttaagct gtgcgttaat ctcttcatca ctaattttct gtcgaaatcc aatcctaatg
     5341 ttgtcattaa tcgttttcca tggcagcaaa ttatgatgtt gaaatagcat taaacaatct
     5401 ggagacggct gttgtttaat ttcgttatca ataatgacac gtccagatga tggatgaata
     5461 aatccaccga tgatattgag taaagtagac tttccgcaac cactttttcc tatgaaagtg
     5521 actatttctc ctttgctaat gtccaaatta aagttatgaa ttactttatg tgatccaaag
     5581 tgatgttgta attgttgtat tttaatcatg acaagccccc ttttaatccc tactatttta
     5641 ataggaatta agtgtatatc taaagggcta tgccaaacac agcccttata tcgtgaattg
     5701 ttatatctat agatgattat ggtttggtat gagtgctctt ataacataag cgttattcaa
     5761 ttaacgacgt tatattaatt tcagctctac taaatttcaa taaaatgatt gttttagaca
     5821 atacttgcat ttagttcatt agtttcgtca tattaactac ttacatttat agcattgagc
     5881 tattctaatt aactgcaata gaaatccacg tcaaacctat atacatatat ctcatcaact
     5941 tgttgtctta ttaaagtttt tcgatgccat caatcggtgt atcaactgct ttgcgcgcgc
     6001 gacgtaccgc ttcttcatca ggtgcgttgt ataaacaaat acatttagac atatcttcac
     6061 atacatatgt gcgtttaaat tcaacttctg gcacttcttc ataatgaaca gaatttttct
     6121 ttttacgtgc taaatattga tccatcgtaa tgccttccgg aatgttccaa gttacaaggt
     6181 aatcaacatg tgcatcacca tttttaactt catctaaatc tttaccaact aagcgtactt
     6241 ctttgactaa ttgaatacta atatctgctt ctgttaaaaa ttgtttagct tctgcttcgt
     6301 catttgcttc cacaataaaa taaccatgag ttaaattttc agtagcttgt acttcaatta
     6361 atgttggaat tgtcttcgta gatagtgatg ctgcctttgc ttctagttct tctttcgttg
     6421 atgcaaaatc aagattgtta gcttctaata aaaataatgt cattaataag acctcctata
     6481 acaatggttg aaaaaatatt actataattc ttatcggatt acaaggatat tgagcgattt
     6541 ttttaatatt tagataaaat cctgtaaaat atattttcaa agagtataaa aatcatgtta
     6601 gggtataagt aaataaacat atatataggg ggctatcatt atggataaag aatctgtcgt
     6661 agcaagttta gctagaaaca aaaagattgc tgtagaaaca atggccggtc aaagatacat
     6721 catagaacgc attttacata caaatgatga aaaacatatt catattttga aacctaaaga
     6781 tgtcgtgtta gatgtagata gcatcaaaga aattgacgaa aatcatttaa atgatgcaac
     6841 ataatccaaa acaaacgtgg gagtgggaca gaaatgatat tttcgcaaaa tttatttcgt
     6901 tgtcccaccc caacttgcat tgcctgtaga atttcttttc gaaattctct gtgttggggc
     6961 ccacacccca acttgcattg cctgtagaat ttcttttcga aattctctgt gttggggccc
     7021 acaccccaac ttgcattgcc tgtagaattt cttttcgaaa ttctctgtgt tggggcccct
     7081 gactagaatt gaaaaaagct tgttacaagc gcattttcgt tcagtcaact actgccaata
     7141 taacttcgta gagcatagaa cattgattta tgtcccagcc tgtacaatgt tttatttatg
     7201 ttaatttgtc gaagtatggc tcatctaatg gattcaattg caagttttca atttgcaacg
     7261 ttgaatgtgc aatgccatat ttttctttca acaatgatga tacttgatca atcgcttgat
     7321 aatcatcacc ctcatatttt ttatctaaca caacatgggc acttaatgaa taatgctctg
     7381 ttgtaatact ccacaaatga aattcatgta catctaatat gccatctatg tttttaatat
     7441 ctgccataat ttgatcagta tccaaatgtt gaggcacact ttccattaaa attaaccacg
     7501 cattacgcgt aattttataa ccaccacgta aaatgatgag tgaaattaca atactaatga
     7561 ttgggtcgat gatgcgccat cctgtaaagt aaatcaatac aactgcaacg atgacaccaa
     7621 tagagttcaa taagtctccc atgaaatgcc ataatgcact ttgaatattg atattgtctt
     7681 cttgttttaa agaccttaca aggataattg tcaaaataat attaacgagt aaaccaatac
     7741 tagcaatcat aaacataatg ccactttcaa ttggttgcgg ataaataata cgtacaatag
     7801 cttcatataa aatccagatt gaaattacaa ttaatgctaa accatttaaa aatgcagcta
     7861 atatctcaaa tcttaaatat ccaaatgtgt atcgtgcagt cggcttttta cttgcaaaat
     7921 aaatggccaa catagataaa ccaagtgcta atacatcact aagcatatga aatgaatctg
     7981 acagtaatgc caatgaatta gatactaaac ctccgacaaa ttcaatcact gtaaataaca
     8041 atgtgatgat aagtgatgcc cacagcgtct ttttagaact ttgttgaatt ttttgatgat
     8101 tgacatgatg aaaatatttg tatctgttta tcgtattcgc ctttttccca ttcatacgtt
     8161 tgccactcct aaataaatgc gtcgtattta atattgtcgc ttttaaaaat aattctttat
     8221 agcttctcag gttaatatta atgcttccgt aattaaccat gatttataca tcatcgtcat
     8281 ttacatgaca actcattaat ctttaccctt tatagcttta tgtaatatca ccttggaaaa
     8341 cttttacagc acactcgctc tttgctttat tcctatcata tagaaaattt taataatgta
     8401 cgagattatt gtgttagtta cataaaaatg acaaaaaagt atctcaaatt tattgtttag
     8461 tttcattaag ttcaaaaaat cgcaataact ttaatgattg ttccgcatat taaaacaaac
     8521 gatgactttg catattgaac ttcatatttc taaatcacca taattaataa acaactctgt
     8581 ccttataata gccgcatcgc aaattagcat aattaaaaac agcagtaaga tgatttgcaa
     8641 ttaaaaacca tcctactgct attattttag tcattatatc ctaatatcat gatacgtgtt
     8701 cctatatatt ttgttttgct taaaatctaa acaacgccgt taaatgttaa gacttaccgc
     8761 tagactgacc tacaaacacg cttcagaaac gatatttatt ctagtacaaa ccttttaaag
     8821 cattgcttgt atcaatataa atatttttaa cttgttgata gttacttaac gcacctttat
     8881 aagtttctcg accgatacct gattttttat aaccaccaaa tggtgcgcct tctggtactt
     8941 ggttgtaagt gttaatccaa atacgtcctg tacgtacagc tttagcaata tttaatgcac
     9001 gtgtgatatt ttgagaaaat acaccgcctg ctaaaccata ctcagaatca ttagctatat
     9061 caattgcttc ttgatcgtcc ttcactttaa tcactgttaa cactggtcca aatatttctt
     9121 cttgtgctaa tttgtgatga ttgtctggca cagcaattaa tgtcggctca aagaagaacc
     9181 ctttatctaa tccattttca gttaagcgat gaccacctgc taaaatttgt gcatctgatt
     9241 cttttgctgc atcaatatat gattgaattt tatctaattg atccttacca gtttgactac
     9301 ccatttgtgt agcttcatct tgtggatctc caactttaat atttgaaaat gcctcttgta
     9361 aacgtggcac caattgatca taaatttttt catgaactaa taatcgagaa cctgcactac
     9421 atacttcacc ttggttgaat aaaataccta actgaatacc ttcaactgca aggtctaaat
     9481 tagcatcatc taatatgata ttggcgcttt taccaccaag ctctaatgta gcgggtacta
     9541 gatgttttgc tgcagcttcg gcaacttgat aacctacatc agttgagccc gtaaatgata
     9601 atttatctac accatcatga ttgaaaattg cattacctga ttctgaacct ttacccgtta
     9661 gtatattgac aacaccttta ggtaatacct cttggaaaat tttagcaact tccaataaac
     9721 ttaatggtgt tgaagacgaa ggttgaatca caattgtatt acctgcagca atggctggcg
     9781 caatcttcca tgcagctaat agcattggga agttccaagc aacaacagca cctacgacgc
     9841 caatcggctc atgtcgtacg atactcattg tgtctttatc gatatcattt actgtacctt
     9901 cttctgtttc aataacactt gcgaaataat ggaaatgtct tgcagcaaat ggaatatcaa
     9961 ttgctgttgt ctcacgaatc ggtttaccat tatttaatgt ttcaatcatt gcaattttat
    10021 ctttttgtgc cattaattta tcaccaatat cacgcaacat ttgtgcacgt tctgatttag
    10081 aagttaatga ccatgattca aatgcatctt gcgccacttt gactgcatga tcgacatctt
    10141 tatcttttgc tcttgtaata tgtgatagtg tttctccagt tgctggatta gtcacttcga
    10201 ttgtttcgtc actgctacct ttaacaaatt ccccattgat aaataaacca taattctctg
    10261 ctatataatc tcgaacgttt actgccatat tatctctcct ttgtttattg gctataatat
    10321 aaatctctta gcatcatatt taataatgct tttaaacgat atgattcaca ttatatagta
    10381 agcgtttaca ttttataaaa taatcagctc acagaagtct catttttaaa acttttgcgt
    10441 cgtttttgca cataaaaata atcttatttt cgaaaacata ttgcactttt caaaaataaa
    10501 acacataata taactataat acttgagaat aattctcatt aaatagtttg gttgttatga
    10561 aagtcgcgct taaaatgcgt taatgtgaca aggataattc aacatcgttt cattttactg
    10621 agtcattgca cttatcatac acattatatt tagcatgagt tatattacta aaacaatatt
    10681 acatgcgcat tagtactaga atggagtttt ttcaatgcga cttattttaa tcgtcattgg
    10741 cttaatattt acagcgcttg gtattgcagg tgccgtatta cctttactgc caacgacccc
    10801 ttttttactc gtagcagttt tttgttttgc tcgaagttca gatcgctttt acaattggct
    10861 tattaatcaa aaaatttata aagaatatgt agaaaacttt tatttacatc gaggctacac
    10921 gctacaacag aaaattaaaa ttttaattag cttatacatt gtaataggtt tttcaattta
    10981 tatggtggat gttcttgcag tacgtatagg attaatcata atggttatca tacaaaccgc
    11041 tgtactcttt acatttgtaa aaacattacc caaatcaaat cataaaatag aggagtgatt
    11101 gcccatgttt atggcagaaa atagattaca attacaaaaa ggcagtgcgg aagaaacgat
    11161 tgaacgtttt tacaatagac aaggcattga aactattgaa ggcttccaac aaatgtttgt
    11221 cactaaaaca ttaaatactg aggatacaga cgaagttaaa atcttaacta tttgggaatc
    11281 tgaagatagc tttaataatt ggttgaattc cgatgtattt aaagaagctc ataaaaatgt
    11341 acgtttaaaa agtgatgacg atggacagca aagtccaata ttatcaaata aagtattcaa
    11401 atatgatatt ggctaccact atcaaaaata aatatattaa aaacccctag tcaccgctgg
    11461 ttgtctaaac caacttcatg attaggggtt ttttattatt tgtgacgtaa aggtacgaat
    11521 tcatccggct tgtctgtgcg caatccaaaa taatattcta ttgcttcaca aatacgtcgt
    11581 gatgcttgtc catcaccata tggattattc gcttgactca tacgttgata tgcttcatca
    11641 tcctcaatca attgtttcac atttcgaaca atattgtcat aatctgtgcc aattactctc
    11701 gatgttcccg cctcaacgcc ttcgggacgc tctgtatgat tccttaatac caacacaggt
    11761 tttccaaatg taggagcctc ctcttgaata ccaccagaat ctgtcagcac gaggtacgat
    11821 tgatttgtaa aattatggaa ctcaatcgca tctaatggct caattaattc aatccgattt
    11881 ctcccagata aatatttttc ggcaatcgct cttacctttg gattacgatg cattggataa
    11941 atgaagacaa catctttata ttcatctgcc aaatctctta ctgctttaaa aatctgatgc
    12001 atcggttccc caatattttc acgacgatgc gctgttagta aaataacttt cttgccttta
    12061 tgtttattaa taatcgttga aacaaaatca ttttgaactg ttgttgacaa tgcgtcaata
    12121 actgtatttc cagtaataaa gatacgctct ttgtctttgt tttcaaaaag taaattttta
    12181 gctgcaatta ctgttggcgc aaaattcaat tcagcaatat tacttaccat gactcgattt
    12241 aactcttcag gaaatggtga gtatttctga tgtgttcgaa gtccagcttc tacatgtccg
    12301 accggaattt gatgataaaa tgctgccaaa cttcctacaa aagtcgttgt agtatcacca
    12361 tgtactaaaa tcatatccgg ctgttcctca ttaatgatgc tatcaagttt agcaagcgca
    12421 ttagccgtaa ggtccgctaa cgtttgttga tcttgcataa tatttaaatc atgatcagct
    12481 tgaatatcaa atatacttaa cacactatct aacatatctc tatgttgtgc tgtaatcaca
    12541 atgttcgctt caaagttccc attatgatca atttctttta ctaatggtgc catttttatt
    12601 gcttcgggtc tcgtaccgaa aataaccata atttttttca taacattatc ctctctgaag
    12661 ttcaaacaca tgacagctta tctttcctta atcatgtatg ccctagtttg acacatttta
    12721 tttgtcgata aaattaaata tattgccata attataatac gatacatctt caaatgatga
    12781 tttcacaaca ttttttgtat caaaaatcac tttatgcttc attttatcaa aatgactgtc
    12841 cgataaattt ttaaattctg agtggtcact taaaatcaat actagcgatg cgtctttgac
    12901 agcatgtgac atatcatgtt ccacaaaatc taattcaaca tgtggatcat aagcacatac
    12961 ttctatgtct ggttcttgat ttaatagctc ataaatatca aatgctggcg attctcttat
    13021 atcatcaaca tcacctttat aagttaaacc aaatactgtg actttattcc cgctcaatgc
    13081 tttgatgatt tgcttcgttg tatcaacaac ataggccggc attgaattat taatttcacg
    13141 tccagtttga attaactttg cattttcagg gtctttagca ataataaagt acggatcaac
    13201 agctaaacaa tgaccgccta cacctggacc aggttgatgg atattaacac gcggatgttt
    13261 gtttgccatt tcaatcacat ctaatacatt aatatttaag ttgttgcaaa tttttgttaa
    13321 ttcattagct aaagcaatgt tcacgtctct atatgtgttt tccattagct tactcatttc
    13381 agcagtacgt gcatctgttt caatcatttc tccctgaaca aatgtgcgat agacacgttt
    13441 acccgcttca atacaagctt tagtcacacc gccaatgatg cgattgttat gaactaattc
    13501 ttctaaaatt tttcctggca gtacacgttc tggacaatgc actaaataaa tatcttcacc
    13561 tattgtaaac cctaaatttt caatgactgg ttttacaaaa tcatccatcg ttttaggcgc
    13621 aattgtcgac tctacaataa tagtattccc tttttttaaa aatggtaaaa tactatctaa
    13681 tgcacgcata actagcgaaa tgtcacatga ccggtactga tcatcattat tcggcgtcgg
    13741 aacggcaatg ataaaaacat cagatgcttc tggcgttgta gataccttca attttcccga
    13801 tgacagtacc tcttcataaa tctcttgtaa tccaggttct tcaatactaa tttgaccact
    13861 ttgtaactta tcaatcgttt gctgattaat atcaacacca agcacatcga cgccatgctt
    13921 tgcaaacata attgatgttg gtaaaccaat ataacctaag ccaactactg ttaacttcat
    13981 actataacct ccaaataaaa cgatttgtat gttgatttta tttaatagat gacttatgcc
    14041 ttatctttgg ttgtcgtttc atccatgatg tccgcaataa caagtgacat ttttccagga
    14101 ataacttcaa gtgcattatt attttcatat aacgtattgc tgtatattaa attgccgaat
    14161 aattttctaa agaccgacaa cttattaaaa tacttattta acactgaagg catgttgatc
    14221 aataccgttt tacgatgtga ttggcgacgt atttcataca ttactgacga tgtatcaaag
    14281 taaaagctat cttgaggatg atacacgcct gtcacttcta atgatattaa ttgatcaata
    14341 aatgctgtca gatgtttaat atataatgca ctgcgctgat tgttaatatt gggaatgatt
    14401 ggcagtcgct ttgacaattg cattaaccgt tggaaatttc ctgggcaatg tgcaccataa
    14461 atcattggtg gtctcacaat tgctacttta aacgaatcac taatcaactc ttgtaatgct
    14521 tgttcagcga actttttgga aataccatag ttggtcgtag ggttcattgg tgtttgtgta
    14581 tcaatttgat ctgatttacc aacctgacct tcttttccat aaactgccat agtactcata
    14641 aaaataaatt gtttaacgtc ttcagattta gccttttgtg ccaattgttt cgtcagcaac
    14701 atattcactt gcatataatc agatagcctt gcttgaggtg aattgttgtg aaccaaagct
    14761 gctgtatgaa ttaaaacatc ataatctttg aacgaggtcg acttccataa ttgattccta
    14821 acattaattt gatctacttg atgtccttgt tcaataagct tatcttttaa agcattaccg
    14881 atatatccat gtacgcctgt aattaaaata ttttttctca ttagtgatgc acaccttctg
    14941 aagtaacgat atttttaatt gttttatata tgatatacat atcaagcatc atagattgat
    15001 gtgttaagta ataatgatca tacgctactt tttgatcatc agtgatatca tctctcccca
    15061 tcacttgagc tagtcctgtc acacctggtc taatcgtatg cacgttcact tttgtacgtt
    15121 tttcgattaa ttcgtattga ttataaagcg ctggtctagg acctacaatt gacatttctc
    15181 cttttaaaac attcaataat tgtggcaatt catcaataga ggtcttacga atgaccttcc
    15241 ctgtctttgt tatatacgat gttgaatcca ttaaatcagt tgcaacatta ggtgtgtcta
    15301 ttttcattga tctaaactta taaatattaa acaattcatt attaatcgtc ggtctttttt
    15361 gtttgaaaat ggctggtcca ggtgattcca ttttaattag taatgctgta attaacagaa
    15421 tcggacttaa aactactaaa ccatatattg aactcactac atcgaataat cgcttcataa
    15481 ttatctcctc acttcaacag attgtaatac ttcatagtat taatttctct cgtatattta
    15541 tctttaaaca gttgatgtgc gcgctcaccc atctcgtgca attccttact cgagtgagtg
    15601 ttgatgaagt tataaatggc atgtgcatcg ccattatcaa tttggatacc cgcatcatat
    15661 tgttcaacat gttgaacgat atcagattgc ttatccatga tgagtaccaa cgctttctta
    15721 gctgcaagat agccataatt cttgctcggc acgcctaaac cgacgccttc tttaatcagc
    15781 gatgcaatac atacatccgc aatttttaat acgtcagcat agtctgtacc tgttaaaaac
    15841 tcaaacattt taacattttc aatacgatgg tcttctattg ccgttttgac atctgcaaat
    15901 ttcttaccat gaccacaaag tattgttaac gtttgaggct gatccttatt taattttaaa
    15961 aatgagataa gtgtctccat atcctgtaac tgccccatat taccgctata caataaaatt
    16021 ttgtcgtatt gctcacggta agctttaaat gtgtcattat agatacgatt gtcttgtaat
    16081 tgacgcatgt cataccagtt aggaatcaca tggatattgt cagcattttt agaaatttga
    16141 tgatttagta agtagttttt catttccgta ccaaggacaa tgacattttc agcattcttg
    16201 tagacatgtc tattaatgta acgcatcagt ttatcaatca tgctacctgg acgagttgca
    16261 cctgtcttaa tcgcattatc aggtgctata tcatacacca caaaagaata ttttttctta
    16321 agcagtctgt gtaaaacgtc tggtattaat ggcaagattg gtggattaga gtaaacaaga
    16381 atctgatcat atttcaacat tttaggtata ttaatcacga attttgaaaa taaactaaag
    16441 aaattgatga tccttccaac cttacttttg ttattaaacc tcgaatactt gagacgtcga
    16501 atgcaaatac cacgatgcat ctcggtttta gaaacctgtt tatgattact atattcatat
    16561 ggccatccac acatgacatc gacattaatg tgattcgcaa ttaaatcttc cgccaattga
    16621 gttggtaacg tcgcagaaga tacatattcc ggataaaaat actgacataa aatcaaaatc
    16681 tttttttcac tcatctaatc gacgtccttt ttattaatga atttagacta agaatttttg
    16741 aactgtttat ttttagcaaa ataatagagt gctatgaaaa atatcatgta cgatacgatt
    16801 aaaacaattg tattaataga tacaaagttt aaaaaagtta aattaagcat ttttgatata
    16861 acaaatgttg cagtagtacc tattaaaaat aacgcttgta gtattaattc taactgttgt
    16921 ttttgtaata caatcacaga aagtgatacc gttgatacaa tcagccttat aacaaataat
    16981 gggataacaa tttttataac tgtaattgcg tcaacccagc tatgtcctaa taatatattg
    17041 actaattctt cactgaacaa ataaagtggc acataaagaa ttatagaaac tataaccagt
    17101 atggaactaa atttaagtaa taaatttcgg aagttaccat gttcaatata atagtcattg
    17161 gcttgttgca taaatatttt tgataaccct aatgaaataa ttgtcactgg tatgcctagt
    17221 actcttatgg ccataccata aatacccact tctgtattgg tatacaaaat acctataaag
    17281 aacacaacaa ccgagaaaga caaactattt aataatattg atggtgttga ataaactaac
    17341 tgatttttat tttctaaaaa taatgcttta gtttcttctt tatctctcac aatattaaag
    17401 tgctttttaa acgttttata tcctagtgta atgcctgcga tataggataa tgaaaaacca
    17461 ataattaatc ctaaacttcc taatgctaaa agtccgaaaa tgatttgaag taaagccatt
    17521 gatccagctc taaacacatt aatcaaactt aacactttat actttctttc cttatttaga
    17581 taatttgtaa aaatgtttgt taaacttacc aaaaacaaca taataataat gaaaataaaa
    17641 tctaatataa agtttgggta taaacatgca ctaatcgcaa atattggtat cagtattaat
    17701 aaaataagca atgatatcaa aaaactgatt tgtgaaagta tattagcact acggtcattt
    17761 ttggtattca caatcaacaa atcgtatctt gcatttatta ttggtattaa aatcattgcg
    17821 atatttgaaa aaattgtaaa ctcaccaaat tctgtaggtg aatatagtct tgtaataatt
    17881 ggggtagtga tgattaatat gacttgagcg atgccactgc ttaaaatcat taaaaacgaa
    17941 tcgccaataa atttatttaa tctcataatt aacctcagat tttcttgatg attttcgctg
    18001 gattgccacc tataacaacg ttaccttctg gaaaactttt tgttacaacc gacccagccc
    18061 ctacaattgt atgttccccc aattctactc ctggtaatat aactgagttc attccaatcc
    18121 aactataatt ccctattttg acatcttcac ctggtgcatg tgacttcaaa tttttaatat
    18181 tatgattagc tgtaataata cctacgttag gcgctatata aacacctcta cctatatata
    18241 taactgctga aaaattatta aaatacgtcc cgggcgattg aaaaatatga atatcatttt
    18301 tatcaaaaac aatgttatta gggttatgca ttctaacagt tatatctgca ggaaatggca
    18361 aacttgtatt tattcctaac aacttgaacg gaacagcttt ccacgcccat aaccatccag
    18421 ccactttatt ttcaaaataa tatccagtta aatattcttt cttataaaat aataatagtg
    18481 gtgtattcat taatctaaat attttcgatt tcataaatgt ttttacaatc attctatccc
    18541 tactttttta gtaaattttt ttattataat gcctgggata atagctataa catatacaac
    18601 taaaatcata gaaatcaatg tgattaatgg tgtcgggtga ctaaaaatac tcgtaataag
    18661 tctcatataa atatatgtac caataaatat cacttctaac gaatgactct tccgaataat
    18721 atactcactc agaaatgcaa aaaagatatt tgcaattaaa ttcaaataga agaataaggg
    18781 tccaaaataa ataattccat atccagcact cgaaattaga tgaccagtca attgtttact
    18841 accatatatc aattggttaa aaagttggct agtaatcaac tgctttttat ctaaaaagaa
    18901 attaaaccca aaaacagctc tggtattatc aaataaatat tgtttgaacg aaccattata
    18961 gtaattcaaa tagtctatag aagctgcaat attgctaggt ccataaaaat atgactgcaa
    19021 cgtgtcaact attttaagat tacttacact tgtactattt aacgcctcgc tatatgaact
    19081 ataattaaac acatacaatt ctttatatag tgtcatcaat gctaatacga aaatacctac
    19141 agatataata atgatattga tacgtctttt atgcttagaa aacaagattg acaccactgt
    19201 taaaacagca accattgtat aaagttgata acttcttctt tcaccaacaa ttaaactaat
    19261 atttaaaatt cctatagcta gcggtaatac aacataaata attttaggat tctctttata
    19321 ctttttatat gaagcatatg cgattattaa gaataataac gctaaggcaa gttgaaatag
    19381 cattacaaaa agaacattta aaccacttgt tgcttcggtt cctcttccca ttgcatctgt
    19441 tttaataatt aaaaatgata tgtttttgcg cacttcggga aaagctacaa aaatcccaca
    19501 aataacaaga ccaaacacaa tgtaataaat tgatgatcca gaaagtgtaa agctatttct
    19561 tgataccact tcatgtttga ataatttaga tagtatcaat ataaatagcg agcctatgat
    19621 aaactccaca ctcatataaa taaccgcttg attaaaataa gcttgtccta atggtaaatt
    19681 atctatagtt gaatcctgga aaaacactat catagcaggt ataaatacta atctaagaaa
    19741 acctaaaaca tcgtatacaa ttttcgaaaa tttaaaattc ggattattcg agaagcttat
    19801 aaagagcttt cctactccat aacttatagg tgctaaaaat aataaaggtt tatcacctaa
    19861 cataaggcaa ggaaacatta aaatggcaat aacatatgaa gtgacattat atatcttatt
    19921 cattaaatat cctgcctatc tcttgataga actgtttatt attttcaatt acatcgtttc
    19981 tataagcttc tcttttatga ttaatgtcca ttacatccaa attgttatac caatccatca
    20041 attcatcacc aatacctttt aaattttctg gattaatact aaaacccagt ccgaatttat
    20101 tagcttctgt agcggtgaat gtatcgtctg ttgtaataat aggtttgttt aaaaacaacg
    20161 cataatacat tcttatggat aaagcagtat ctaaagcaat attttgatta ccaaacaaat
    20221 tattaagtat atcaatttca tttaaatatt tcggtgtttc tttcaagtca aagccgccag
    20281 aaaatgtaat attattaata aaattttctc gagcaaagac ttctaatttt tccgaccctg
    20341 ttccaaaata ttgcatatgg aaccttgaat catttgccag ttcctttatc aatttttgat
    20401 ttatctcatt aaatctaaca ttaccaataa aacctatatt aattggccat ctttttttaa
    20461 acccatcggt tacattactt tctctaataa tactcatgtt ataactataa ataattctat
    20521 atttttcaga tttaggtaaa aatttaagaa aaccttctga agataatgtt gtcaccctgc
    20581 tagcatcaac gatttttttc attctatact taataagttt attattttca aaaaagtagt
    20641 cccttatatt taaaatgaat ttatttttat agtgtttttc taaaaaactt ttaaataagt
    20701 gtcctgtata acttccccat actatgacaa aatcataacg attttcttta agaattttca
    20761 tagcgtatgg cctaaatcga tagtaagcaa tagctttttt gataaatgtc caatcttctt
    20821 taatatctac tttatattta tagtaattat tgatgccttg gatatgttct tcgatatcat
    20881 atttgtcagt gtatataatg tccacctcat tgatattcaa atcaatgtgg tttaaataat
    20941 gtgataataa tgacatatgc ttaatgttag tagcgccaag aatcgctatc ctcatcgtca
    21001 tttcctccaa gtatttcgat tgataatatc tttatagctt tgaataattt taactacctt
    21061 tgtcgaaacg ttagtgtctt tataatcaat agcatcaatc atcggttcgt tattgttttg
    21121 catctctctt gctagttcaa cggattggat tagattgtta taggtaatac cacctacaat
    21181 aaccgtacct ttatctagta cttccggtct ttctgtggaa gttcgaataa ggacaccagg
    21241 gaacttcaaa atagacgact cttctgacaa tgttccacta tctgatagca caacaaatgc
    21301 atctttttgc aatgcattat aatcaaagaa accaaatggc tttaactttt taactaatgg
    21361 atcaaattca aatttacttt cttcaatttt cttccaactt cttggatgcg ttgaataaat
    21421 cacaggcatt ttatactttt tggcaatatc atttatcgca ttcattaatg atttaaaatt
    21481 cttttcatta tcgatattct cttctctatg cgcagatact aaaatgtatt gttgcggttc
    21541 taatcctagt ttatttaaaa cgtcactgtg attaatttta tctcgatgcg cttctatcac
    21601 ttctgtcatc ggtgatcctg tcacaaagat attcgcttta ttgaagcctt catctaataa
    21661 ataacgtctg ctatgttccg tataaggtag attcacatca ctgacatggt caacaatttt
    21721 acgattgatt tcttcaggta cattctgatc aaagcatcta ttacccgctt ccatgtggaa
    21781 cacaggaatc tttaatcgtt tagcagatac tgctgctaaa caactatttg tatcaccaag
    21841 aattaaaagt gcatctggtt gttcgcgtaa taaaacgtca tatgtcttcg caataatatt
    21901 ccccatcgtt tctccaaggt tacttccaac tgcctctaag tagtggtccg gttgtctcaa
    21961 ttccaaatca tcaaagaaaa tttgattcaa tgtataatca taattttgac cagtgtgtac
    22021 taatatctga ttaaaatatt gatcacatgc tttaatcgtt gatgataaac gaatgatttc
    22081 aggccttgta ccaactattg tcattaattt tagtttttcc atgcgctata cctccaagaa
    22141 atacgtatct ggctgatttg gatcaaacat ttcattcacc cacataatag ttaccatatc
    22201 tgtgtcacct aaattttcaa tattatgtgt gtatcctact ggtatgtcta caacttctaa
    22261 tttgtcgcca gaaacgtaat actcaatgat ttcatcatca ttaacatgtc taaaacgaat
    22321 taccccttta cctgatacga ctagaaattt ttcgttttta gtatgatgcc agtgattacc
    22381 tttagtaata cctggcttag aaatatttac agaaacttga ccacgatccg gtgtttttat
    22441 aaattctgta aaagaacccc tatcatccac attcataagt aagggataac taaagtctgt
    22501 actaggtaga tagcttaaat acgtactata caaatctttt tcaaacaagt tatctaattt
    22561 cggcaatgtt cgatcgagac gtgactgttt gaacttgtat aataaatcta caatttctcc
    22621 caatgtcact ttaaatacgt ttggtactgt aggtacacca ttttcaatcg ttggagttcc
    22681 ttcaatagca cgctttattt cagcgacgat atcatccacg tagtttagcg ttagttcaac
    22741 attccgatca ttaacttgaa tctcttcgtt acgtgcaatt ttgtaacaaa atgttgctat
    22801 cactgagtta taattcggct tacaccactt gccgaataaa tttggccagc gataaatata
    22861 aaccgtattg ccatactctt cggcatactc tcttaatagc tgttcccctt gcaacttact
    22921 ctcaccataa ggattatctt gtgttgcttg tattgaagac gataataata tcgctggctt
    22981 tttcgtattt ctagttaata tatcaagtac atgatctaaa taactcacgt ttcctaagct
    23041 gaattctttg tcatgttcag gtcgattaac acccgctaaa tgcacgacaa agtctgcttt
    23101 caacaatgct gactctaatt cttcctcttt agtttgtcga tgtacttcga aaatatgatg
    23161 atctgtcgtt gatgttaaat ctgctttcaa gttttttcct acaaaacctt ttgctcctgt
    23221 aattacaata ttcaacgtca atctctccta tctcattgaa gctttataat cattcaattc
    23281 gtttctaaca tattctagtg ttaaaagttt ttcttttatc tcttccaccg ttaaaatatg
    23341 tgtattatcg gagttatatt cataagattg cgtaatcttt tcgttaccgg tttcaacata
    23401 attactataa tttaaatctc tggagtctgc cggcactcta aaataatcac ccatatcttc
    23461 acattgtgcg tattcttctc tcgtcaacaa tgtttctgct tttttctctc catgtcgtgt
    23521 accaatgatt tcaattgcat tatcagcttc aaacaattct aataatgcgg tcgcaagatc
    23581 ccctaccgtt gagcttggtg ctttttgaac cataatatcg cctgtttctg catgcttaaa
    23641 tgcatgaaca actagttcta cggcatcttc taaactcatt aaaaatcttg tcatatcagg
    23701 atctgtaatc gttaaaggtt ctccagcttt gattttgtcg ataaacaaag gaattactga
    23761 tcctcttgaa gccatcacat taccgtatct tgtaccacaa atcagcgttt gttcactacg
    23821 aatatttctt gatttggcta cgaatacttt ttccatcatt gcttttgaaa tacccatagc
    23881 attaatagga tacgctgcct tatctgtaga taaacatatg acttttttaa cattttgatg
    23941 aatagcactt tgtaagacat tttctgtacc aataatattt gtcttcactg cctcaactgg
    24001 aaagaattca catgacggca cttgttttaa agctgctgca tggaatacat aatcaacatc
    24061 tcgcattgct gtttctacac tttgactatc acgcacatca ccaatgtaga actttaattt
    24121 tgaattatta tatttttttc gaatgtcatc ttgttttttc tcatcgcgtg aaaaaatacg
    24181 aatttcttta atattagaat ctaaaaaccg tttcataaca gcattaccga atgatcctgt
    24241 gcccccagta attaataaaa ttttgtcatc gaacataatt atctcccttt ttgccattgg
    24301 catagttaat aattttttct ttactaaagt cattgacgat gtcttgaata atcgcttcaa
    24361 cttcattaca tttcatatgt tgtactttgc cacgataaat tttttcaaat acttgttcag
    24421 gatgaacctc atctttattc ataagctctt caaacatttt ttcgccgggt ctaatccctg
    24481 tataagtaat gcgtatgtcg tcttcttttt taccacttag cttaattaaa ttacgtgcca
    24541 aatctacaat tttcactggt tctcccatat ctagcacaaa tacttcgcca ccttctgcta
    24601 atgcccctgc ctgcaaaact agtctagaag cttcaggaat tgtcataaag taacgtgtca
    24661 tttcaggatg tgtcacagta actggcccac cttcttcaat ttgacttttg aaaagtggaa
    24721 tcacagatcc tctcgatcca agtacattac caaatctcac tgcaacaaaa tttgttcgat
    24781 gcgtttcatc atttaaactt tgaataatca tttctgcaat tcgctttgaa gctcccatga
    24841 cattaggcgg attaacggct ttatccgtag aaatcataac gaatttcttc acctctgcat
    24901 ttttagcagc ttcagcagta tttttcgtac ctaaaatatt attacgtact gcttcttcag
    24961 ggttctcttc cattaacggc acgtgcttgt gtgctgctgc atgataaact gcgtatggtt
    25021 tatacgtttc cataatttca aacatacgcg ctctattttg cacatccgct ataataggaa
    25081 cgatatcaac attttttccg aagcgatttc gcaattcacg attgattaaa taaatactgt
    25141 tttcaccatg gccaagtaga ataatacgtt ctggatagaa attacaaact tgtctacaaa
    25201 tttctgatcc tattgaacca cctgcacccg taactaaaat agttttattc gtcaattcat
    25261 ttgatatcat atccatatct aattcaacag gatctctgcc tagtaaatct tctacttcaa
    25321 cttttttaag ttggttcact tctaactcac cagacatgac gtcttctata tttggcattt
    25381 tcaataactc aacgccatcc atatggcaaa tattattaat ttctttcaaa cgctcttgac
    25441 caatagttgg aattgcaatg atgatttttt taatcttata tttcctcact agttctggaa
    25501 tatccgcaat tttaccttgg acttttacac cctcagtaat tgtgatattg cgtttatgtt
    25561 cgtcatcatc gactgctaat accggttcaa gtttcatttc gtcacttttc aacatttgtc
    25621 taatcagcat tgaacctgct tgaccagcac caacaactaa agttggcttc ttattaaatg
    25681 acttacctcc aaggtatttc cgataaatac gccaaaataa ccttgagcca cctattaaaa
    25741 tcaagtgcat catccaagta attaaataca atctaaaaaa cggtctattg cctgtaacaa
    25801 ttgtcacgac caccatcgta ataacgatag atgtcgtcac agctttaaca attaaaatca
    25861 attcactcac actggcatat tcccacgctc gatgatacat attaaaaata aatgctgaaa
    25921 tatgatgcga tatgaatagt gatatagctg ccaatattaa taatttgaca gaatatgttt
    25981 tgaaatacgg ttctaaaatg taataactta cgaatactga aaatgtcact atcagtgaat
    26041 cgattaatgc tagtattaaa agccgcaatt tcacagataa atgtgccata aaacccctca
    26101 tttgctgttt ataacccaaa ccatcttttc tgtttataat cttgttgtgg cattcgttta
    26161 ggaatttttt tatcatcaac aactaacttc gcattactaa taaatccgtt catatcttca
    26221 taataatcac gtaatttctt atcattaaat aagtctttca ttaagaacgg tctgatttct
    26281 gtgttatgcg catctgaacc gatgaaatgt gtcagattgt tttcaatcat ttgaattgct
    26341 aattttctaa tttttctacc ggaaataccc gctaatgacg ccgttgtcac ttgacttaaa
    26401 gcacctttgt taattaaatc gtatagtatg tcaaggtttt gacttattgc tttattccgc
    26461 tctggatgtg caataatcgg tacaaagcct ttactctgta attcgaaaaa taattgatca
    26521 gtatagtgtg gaacttcatt tgatggaaat tctattagta aatagcgtga atcattaata
    26581 ccggtaataa cttttcgatc aatatcatta aggatttgat cggtaattct tatttcctga
    26641 ccataataaa actttagatt tagtgcttgt acttcctcta agctttcaat atggtttaaa
    26701 catgatttca ctttttctat aggtgtggta tatcgaggat gtaagtgatg tgatgttaca
    26761 atgatttctg taacaccttg tgttgtcgct tgttttaaaa gatccagcat ctctgtttca
    26821 tttgtcggac cgtcatcgat attaggcaat atatggttat gaatatcaat catacttatt
    26881 catctccata atagtgataa taactagaag atttatcgac ctttgtcttg ttcaaaatga
    26941 cacctagaat gttactgcct gctttttcca taagtgcttt tgctttttta acttcatttt
    27001 tatcattttt ttcactatca attactaaca aactatcttt aatagcacgc gcatatagtt
    27061 gtgcatcagt cacagtatta actggcggtg tatcgacaat aataatgtcg taacgtttat
    27121 taaacagatc aactaattct ttaaaccttt ctgaagctat taactcagat ggatttggag
    27181 gtacagggcc agctgttagc aaatctaaat tttcaatttc tgtcgacgta attgcttctg
    27241 acatagtcgt tcgaccaatg attaagcttg atagtccatt attattttgc tcattaaaaa
    27301 tatagttttg tgttggctta cgcatatcgc catcaataac taatgtctta tagcctgctt
    27361 gtgcataagt aatcgctaca ttcgatacaa ctgtactttt acctgcacca ggcttttcag
    27421 aagtaaccaa taagcgcttt acttcaccat ttgcttttga aaacatgatg tttgaacgta
    27481 tacctcgaaa cttttcacta attgttgatt ttggtttttc atacacaaat agtgttgttg
    27541 tcgtattttc cttttttgac ataagtagca accatcctta attaaatttt tgaattgaac
    27601 ccaatacagg caatcctaat tctttctcta catcttcttc atctttaatt cgcttatcga
    27661 aaattacttt gaagaagata tatataagcg cgacaactaa tcctaaaaag aatgcaccga
    27721 ttagattcac tacagttttt ggtgcgactt taactgctgt accgtctgct ttagataaaa
    27781 ttgatacgtt atccacactc ataatcttcg gaatttgttt acttgtaact ttagcgaagc
    27841 tattcgcaat tttttccgaa tcttgtttat gaccactttt aacttggatg ttgataagtt
    27901 gcgtattttc ttggtttgta attgtcaaca tactcgacaa tttagatggt gaatacttat
    27961 catttaagtc ctttgacacc tcatctaaaa ttctaggact tttaacaatt tctttatacg
    28021 tatttacaag ttgaatatta ctttgaacct cttgtgccat aaactgagga ttgtcaccct
    28081 tagtttgatt cactaagatt tgagtattag cttgatattt aggtgataag acgaaaaatg
    28141 taacaatagc gctaataatt aaaaataata gcggtaaaat aattaaaatc ttcaagtttt
    28201 tttgtaatac ttctttaatt tttgttaatt ctaatgtact ttccattatt tacctccc
//
LOCUS       DAMZLE010000019        28148 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMZLE010000019 DAMZLE010000000
VERSION     DAMZLE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28148
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.019"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..778)
                     /gene="fmhC"
                     /locus_tag="POB92_000798"
     CDS             complement(<1..778)
                     /gene="fmhC"
                     /locus_tag="POB92_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDI6578318.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILIDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIE"
     gene            complement(806..1924)
                     /locus_tag="POB92_000799"
     CDS             complement(806..1924)
                     /locus_tag="POB92_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI6578319.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            complement(2151..3059)
                     /gene="sucD"
                     /locus_tag="POB92_000800"
     CDS             complement(2151..3059)
                     /gene="sucD"
                     /locus_tag="POB92_000800"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDI6578320.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYEALLTVNK"
     gene            complement(3081..4247)
                     /gene="sucC"
                     /locus_tag="POB92_000801"
     CDS             complement(3081..4247)
                     /gene="sucC"
                     /locus_tag="POB92_000801"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDI6578321.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            complement(4356..5123)
                     /locus_tag="POB92_000802"
     CDS             complement(4356..5123)
                     /locus_tag="POB92_000802"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI6578322.1"
                     /translation="MTLTIKEVKQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            complement(5107..5991)
                     /gene="ylqF"
                     /locus_tag="POB92_000803"
     CDS             complement(5107..5991)
                     /gene="ylqF"
                     /locus_tag="POB92_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDI6578323.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQHDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            6392..8998
                     /gene="yfhO"
                     /locus_tag="POB92_000804"
     CDS             6392..8998
                     /gene="yfhO"
                     /locus_tag="POB92_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDI6578324.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGSLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWLLF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNKTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYAPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(9467..9817)
                     /gene="rplS"
                     /locus_tag="POB92_000805"
     CDS             complement(9467..9817)
                     /gene="rplS"
                     /locus_tag="POB92_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI6578325.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(9920..10657)
                     /gene="trmD"
                     /locus_tag="POB92_000806"
     CDS             complement(9920..10657)
                     /gene="trmD"
                     /locus_tag="POB92_000806"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI6578326.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(10657..11160)
                     /gene="rimM"
                     /locus_tag="POB92_000807"
     CDS             complement(10657..11160)
                     /gene="rimM"
                     /locus_tag="POB92_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI6578327.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(11348..11623)
                     /gene="rpsP"
                     /locus_tag="POB92_000808"
     CDS             complement(11348..11623)
                     /gene="rpsP"
                     /locus_tag="POB92_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI6578328.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(12058..13425)
                     /gene="ffh"
                     /locus_tag="POB92_000809"
     CDS             complement(12058..13425)
                     /gene="ffh"
                     /locus_tag="POB92_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI6578329.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(13451..13783)
                     /locus_tag="POB92_000810"
     CDS             complement(13451..13783)
                     /locus_tag="POB92_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDI6578330.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(13770..15020)
                     /gene="ftsY"
                     /locus_tag="POB92_000811"
     CDS             complement(13770..15020)
                     /gene="ftsY"
                     /locus_tag="POB92_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI6578331.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSESSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            complement(15020..18586)
                     /gene="smc"
                     /locus_tag="POB92_000812"
     CDS             complement(15020..18586)
                     /gene="smc"
                     /locus_tag="POB92_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDI6578332.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISGAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAL
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(18733..19464)
                     /locus_tag="POB92_000813"
     CDS             complement(18733..19464)
                     /locus_tag="POB92_000813"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI6578333.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(19580..19813)
                     /locus_tag="POB92_000814"
     CDS             complement(19580..19813)
                     /locus_tag="POB92_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6578334.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(20183..20917)
                     /gene="fabG"
                     /locus_tag="POB92_000815"
     CDS             complement(20183..20917)
                     /gene="fabG"
                     /locus_tag="POB92_000815"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDI6578335.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(20910..21836)
                     /gene="fabD"
                     /locus_tag="POB92_000816"
     CDS             complement(20910..21836)
                     /gene="fabD"
                     /locus_tag="POB92_000816"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6578336.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDNEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(21829..22815)
                     /gene="plsX"
                     /locus_tag="POB92_000817"
     CDS             complement(21829..22815)
                     /gene="plsX"
                     /locus_tag="POB92_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI6578337.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(22820..23392)
                     /gene="fapR"
                     /locus_tag="POB92_000818"
     CDS             complement(22820..23392)
                     /gene="fapR"
                     /locus_tag="POB92_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDI6578338.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(23595..25655)
                     /gene="recG"
                     /locus_tag="POB92_000819"
     CDS             complement(23595..25655)
                     /gene="recG"
                     /locus_tag="POB92_000819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI6578339.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNNSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYGLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(25845..27491)
                     /gene="fakA"
                     /locus_tag="POB92_000820"
     CDS             complement(25845..27491)
                     /gene="fakA"
                     /locus_tag="POB92_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDI6578340.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(27506..27880)
                     /locus_tag="POB92_000821"
     CDS             complement(27506..27880)
                     /locus_tag="POB92_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6578341.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         8406 a         5471 c         3742 g        10529 t
ORIGIN      
        1 tttcgatttt caataattgt atttttaatt tatcttgata ttcattaaga tttatacaag
       61 ctatctttaa cattgcctta tctttatata tttcttgcat tcgtttaaaa taatcttcat
      121 tcatgaaatg aaaaccatgt ttttcttcag ccatttgaaa taatttataa aatcgatttg
      181 tttcttcaat agataaatct tcaaccttaa caccaatctc aatagtcttt tttatatttc
      241 ttctagtttg gtattccatt tcttttaaaa gttgattctc atctttatct tttaaatcca
      301 atacagaaat ccacctaatt tgacttttat ttgaatatcc tgttgtataa ccttgatgga
      361 gatacccaat tttccctagc atctttacta tcactgaatt attataattc tttattatcc
      421 taccatttgc atctcttaaa ttctctatta aatatggatc aataagaata aatactcctc
      481 tatttttata aatgaattta gataattctt taaaaaagta acaaactaat ttagcatcga
      541 aataatcaag taaaggacct ctatgagagt agaaatattt gtagaattta aaaattcgtg
      601 cctctgttaa taaacatgca gctataactt catttttatc attcttcact cccacaatat
      661 gtgcttcatg atttatttta tttctataat tatataattc tatagactgt gtataatgtt
      721 tgaagtgctt tttggtgtag ttggtaaatt cttcttcact taaagttgaa aatttcatat
      781 ttcaatgtcc tgccttcgat ttaaattatg cttttttaaa tggtctaata aaaatcatat
      841 cattttcata attatgaaca actttatgtg caacctctgc tttacgccat ccaccattat
      901 tccagttttg atctaaactt tggaacttca ttaattttcc atcataatca ccatttaaga
      961 caatagctgt atgaccatat cctccaccaa atcttccact aaaaaccact aagtccccag
     1021 gttcagcttt gaaagttggt gtgttgtgat aaattttagc ttcactatta aaattatttg
     1081 catatggtat atctttagct ccatatcctt ttaatccatg accataaaga tgattccaat
     1141 atacattaac taaatcgaaa cattgccatc cataactacc gtcgaaatcc catcctctat
     1201 tctctaatgt tttcaaataa tttaatgtac tgctattact attactttta ttatttgaag
     1261 acactgtttt tggttttggt tctactaatg gtgtcattgg cacttttaat ttttgaccaa
     1321 taaatattaa attaggattt gctatattat ttgtattttg aatatttgaa actgtagttt
     1381 tgtattttaa tgctatagca ctaagtgtgt ctcctttttt tacagtatag atttgtgttt
     1441 ttggagcttc tctaaaactg tagtaaccaa agtaattatt agtaacttta ttcgttttct
     1501 tctgattaga attttgagct tccaagtttg caattttaat ttctttagta ggttcattgt
     1561 tattaataac tagattgtta ccttggcttg agtttttcgg agtatttgaa atctttatat
     1621 cttgattaat ttcatttccg tttgaaattg ctgatttgtt gtctaacttt aaacttgtgt
     1681 ccgatgtttt aacagcacct tcatttttta ttttgtcttt tgtcgtattt ttattagcat
     1741 ttaactctga tttcgcgaat acattttgct cataccctct tgtaaaatct ttagatttat
     1801 caatttcatc tgcatatgct ttgttcgaca tacccaatgc caaaaacata cctattgaaa
     1861 ttgacaaaat tccaatacta acttttctaa ttgaatagcg tactttactt tgttgtttat
     1921 tcatgatgaa acactcctta caataatata caaacattat aacatagtgc gctatatatt
     1981 ttaattaaaa ttttctttgt tttgtaaagc tattgtagga ggtaaaataa ttaacttgtc
     2041 tttccaaaat atgaaaagtg tactaaaatt catcgcacga caaatagccc atttccaata
     2101 cttttataaa gtatggaatg ggctatagcc atttatatca tcttttaact ttatttatta
     2161 acagttaata atgcttcata aatacctgct tctttagcag cttcaattaa tgttgaacca
     2221 atttctgaag gtgttgccgc tgttttcaca ccacaactat ttaatgtttt aattttctct
     2281 tcagcagtac ctttaccacc tgaaatgatt gcaccagcat gtcccatacg ttttccagga
     2341 ggtgctgttt gtccaccgat aaagcctaca actggttttg tcatattcgc tttaatccat
     2401 tcagctgctt cttcttcagc cgtaccaccg atttcaccaa tcataacaac tgctttcgtt
     2461 tcgtcatctt cattgaatgc ttttaaaaca tcaataaagt ttgttccgtt gactgggtct
     2521 ccaccaatac caacagctgt agtttgacca ataccttctt cagtcaattg gtgcactgct
     2581 tcatatgtta atgtacctga acgagatact acaccaacat gacctttttt gtgaatatag
     2641 ccaggcataa taccaatttt acattcatct gctgtaatca cacctggaca gtttggacca
     2701 actaaacgtg tttttctacc ttgtaagtag cgtttaactt taaccatgtc taatacagga
     2761 atatgttcag tgatacaaat aaccatatct aagtctgcat cagctgcttc taaaattgag
     2821 tctgcagcaa atggtgctgg aacgtaaatg actgaaaccg ttgccccggt ttcattttta
     2881 gcttcttcaa cagtgttgaa aacaggaacg ccttcaacaa cttgaccacc tttaccaggc
     2941 gtcacacctg ctactatttt cgtaccataa tcaagcattt gttttgtatg gaaaagggca
     3001 gtagaccctg taataccttg taccattact ttagtattct tatctataaa tacactcatc
     3061 ttagtgctcc catcctttcc ttatgcttct ttgactagtt taacaatttt ttgtgcacct
     3121 tcagccattg ttgctgctgg ttcaattgct aatcctgagt cttttaagat ttttttacct
     3181 aactcaacat ttgtaccttc taagcgtaca actagtggta aagttaaatc tacttctttt
     3241 acagcttcaa cgataccttc tgcgataaca tcacatttca taatgccacc gaaaatgttt
     3301 acaaaaatac ctttaacatt ttcatcacct aaaatgattt taaatgcttc agttactttt
     3361 tctctagtag cgcttccgcc tgcatctaag aaattggctg ggtttccacc gaaatgatta
     3421 atcgtatcca ttgttgccat ggctaaacct gcaccattaa ccatacatcc gatgtcacca
     3481 tctaatgcaa tgtatgataa atcatgttta gacgcttcaa tctctttcgg atcttcttct
     3541 tctaaatcac gtaattctac aacatcttta tgtctgaata atgcattatc atcaaaatta
     3601 attttagcat ctaatgccaa tacatcacca tcagctgttg taactaatgg gttgatttct
     3661 acgattgaac aatctttttc aatgaataca ttataaagtg ctaataagaa tttagctgct
     3721 ttgttaacag attctttagg aatattaata ttaaacgcaa ttcgtcttgc ttggaatggt
     3781 gaaagtccga ttactggatc gatagtttct ttgaagatct tttcaggagt cttcgcagca
     3841 acttcttcaa tctcagtgcc cccttcttca gacgccatca atgttacttg gtcagtcgca
     3901 cgatcaataa cgaatccaac gtaatattct ttttgaatag cacaaccttc ttcgatatat
     3961 aaacgcttaa tttctttacc ttctggacca gtttgatgtg tcaccaaagt tttccctaat
     4021 aattcttttg catatgtttc tacctcagat aaagatttag caatttttac tccgcctgct
     4081 ttacctctac ctccagcatg aatttgtgct tttacaacat aaacatcaga atttaattct
     4141 tttgctttct ccaccgcttc ttcagcagta aatgctactc gtccttctgg aactgcaacg
     4201 cccattgaac gaaatatttc tttaccttga tactcgtgga tattcatctt ccatcctcct
     4261 gttacttagg ttaagttccc ttacaattat aaaaaatgta agcgctattg taaacttaaa
     4321 tgctactttt ttatcattta attgaatttt acgatttaca gtaacgattt tataggttca
     4381 aagctttttc tatgctcttt cataatgcca atatcatcga ttgctagtaa atgttgtttg
     4441 gtaccgtaac ccgcgttttt ttcaaaacca tattcaggat aatctttaga taactgtgtc
     4501 atataatcat cacgaaaaac ctttgccatg atacttgccg ctgcaataga tacacttctt
     4561 gcatcaccct tgattaaaga tacttgaggc agtgcattat caagcgtcat cgcgtctatc
     4621 aataaatgcg ttggttgtac tgataatcca tcaatagctc gctgcatggc gatttgagta
     4681 gctttataaa tattaaattc atctatttct tcagctgtcg cgataccata tgcaaaagca
     4741 gtaacttcat tttttagtgc ttcatttaat tctagacgtt tcgtaacagg tacttttttc
     4801 gagtcatcaa ggcccaaata attgtgattt gaatttaaaa ttgttgcgca tgcaacgact
     4861 ggacctgcta aaggtcctct tccaacttca tcaataccac aaataattgc attaggatgc
     4921 tcttttaata tttcattttc aaagtaagtc atttcaacat acttttcttt taaagcttgt
     4981 tctttttcta acgcttttct gcgcctagct atggcatttt gaacaccttt tcgctcatct
     5041 aaaaagcatt catgattttc taattcttct attgtattaa ccgcattaat caactgctta
     5101 acttctttaa ttgttagcgt catttgctaa ttcctcagtc atatctttaa aaatatcaaa
     5161 acaataattt cctattttag catttcgaat atcataaata atcagttcaa tgactgcttc
     5221 gtaatcaatt tcattaccac gtcgaattaa gccacgtttt ttccctatcg catcaaacca
     5281 cgctatgatt tctgcatctt caggaacttc aatattataa tgtgacttta atcgcgctaa
     5341 atcatgttga attaaaaagt tcaatccata tatagctact tcatctaagt gcacaatgct
     5401 atctttaatc gcgcctgtta aactcaactt cttaccgact tcttcatctt caaatttagg
     5461 ccaaagtatc cctggtgtgt ctaatagttg taatgcatta ccaactttaa tccattgttg
     5521 ttgtttggtc acacctggtt tattaccagt ctgcgcaata ctacgctttg ccagtttatt
     5581 tattaatgtg gatttaccaa catttggaat tccaacaatc atcgctctaa tcgctcttgg
     5641 cttaagtccc tttgcttttt cacgttcaaa tttttcagca gtcgctttaa ttgctgcagc
     5701 ttccactttc tttaaatttt taccgtgctt agcatccacc gatacaggat agtatccttt
     5761 atcaataaaa aattgttccc attttgacat ctcatttaaa ttagacatat cttttttatt
     5821 taatataaca acacgtggtt tttggttaat aacttcatct atcatagggt ttcttgaact
     5881 atatggaatt cttgcatcta ctagttcaaa cactacatct acttttttta attgttcact
     5941 tacttctctt ttggctttcg ccatatgtcc tggataccat tgaataacca ttcaattcac
     6001 cttactttcg tcaattcttt tctcattatt aaaatctata acctgttaat tatagaacaa
     6061 attcattaat tcaactatat ttattttaac attgtcacac ttcttaacaa tgctttagat
     6121 ttaggtataa gtgattattt caagttttct acaattaaaa atcatcattt gttaaatcta
     6181 agttacttat taagtgacat tcatctttat cagtatgaca tttttattat tcaatatatt
     6241 tgtttaatga taaactgaaa atgatccaaa gtttaagtaa ctatgataag gtatctgttc
     6301 cacagtcatc ttcgtcttta cctatcaaga acctcattaa ctttagtttc agttaaactt
     6361 aattttaaat ttgaaagttg aggataatgt tgtgttaaaa aagtggctaa attcaaacgt
     6421 caaacaattc tttgttataa ctttcattag tgtaatatta acgcttattt tattttctac
     6481 tcatatctat gattatattg tgaatggtac tgtttttagt ggggctggag atggattccg
     6541 tcaaatgatg ccatttcaaa tgtatttgta tgaacattta agtagttttt ctagtttata
     6601 tgatgcatcg tttggattag gtggcgatta tatgaaagga ctatcatatt attattcgct
     6661 gtcaccttta atgtggctaa attttctatt cattaaaata ggagaaacgg ttggtatatt
     6721 taatccgaca acaatacatt tttggccgac aaaccaactt attatggcca tgataagagc
     6781 tatcataaca tttgtcgtga ccttctactt atttaaaata ttacacttta aacgctcaac
     6841 aaatatgatc gctacgattt tatacggcat gtcaactgtc gttatatact ttaattttac
     6901 ttggtcattt tatggaagtt tattatattt attaccatta tcgattcttg gtttggaaag
     6961 atattttcaa caacgcaaaa tcggtatttt cattgttgcg atagccttaa cactatttag
     7021 caatttttat ttcagttatt atcaagctat tattataggt tgctactatt tatatcgact
     7081 cattttcact tacaaatatg acattgtctc tagaacacaa aaattaattt gcgtcatatc
     7141 tgctacagtt ttgagtgtgt tatcaagtgt atttggttta ttcactggca tttctgcgtt
     7201 tttggaaaat gacagaaagc aaaatcccaa tgttgatata ccgtttttga caccacttga
     7261 ttaccattat tttttcttta gcgatggatt ttatattacg atttcaattc ttactatcgt
     7321 tgcattattg tcattcaaac tgtatcgttt ttacttttat agacttttcg caatagtaac
     7381 atggttatta tttattggtt cattatcaca gtatttcgac agtgctttta atggtttttc
     7441 atttccagaa aggcgttggg tgtatatctt agcactatca tcaagcgctc tttgtggatt
     7501 gtttattcaa catttatcaa cattaaatat gaaatattat ttaatcagaa caataccagt
     7561 aagcatcatc gcattacttt atgtattact atcaccgaca cacccacttg cacttatagt
     7621 aggtattatc ctgctaatgg tgcttgccgt tattttaaaa tttagtttat ggcgttataa
     7681 aaaattaacc gttgcaatat tagtattaat cgttatgatt caacaaatcg tcattttaga
     7741 taacaataaa aatatggcaa tcaaacctta tcaacaatca ttatcaacgt tgaaacaaca
     7801 tgattaccat agtaactatg taaaccagct tataaaaaag ataaatcaaa atgcaactgg
     7861 tccatttaat cgcattgatt atatgtcaga ctatgcatta aattcaccat ttatatatca
     7921 ttataatggc atttcattat attctagtat ttttaatgga gacatattaa aatattatga
     7981 caagacactc caaattaata tgccaatcga taaaaacagc acttatagat tacttggcaa
     8041 tcgtcaaaat ttactatcac tttggaatgt taatgatcga attagagtga atcatgatga
     8101 caacttacca tatggattta aaattaagtc tgaacacaaa gacaataaag ttcgttggat
     8161 tcattctaaa aataccatcc attatccaag tgcacatatt acaaataagg tcttttccaa
     8221 taaagaatta aaatctccat tagataaaga acaagcaatg ttgcaaggga ttgtttctaa
     8281 caagactaaa gatgttaata cacattttaa agccaataaa aatttactat cagattcaac
     8341 aattaaatta aatagtgcag cctggcaatc tcctacaaaa catttattac aagttaaaca
     8401 aaataatggt ggtctaactg tacagttgcc aaaatcagtt tctaatcaat ttaaagattt
     8461 gtattttgaa atggatttag aattactttc gccggataaa gctcatgatg ttaaagtgaa
     8521 tgaatataca caagaaagaa ataaactcac ttataaatat cgacgctttg taacacccgt
     8581 aacaatacgc attaaagctt cagataggat tagattatca ttgcctaaag gtaagtatcg
     8641 tgtaaattta aaagggatat acggcgaaga ttataccacg cttaaagacg cttcaaattc
     8701 attagaggct gtcaaagtta gtaagacaaa gcaaggttat actattacta aaaataaaaa
     8761 ttcatctggg tatattgttt tgccaacagc atataatcaa ggtatgaaag cgacatcagg
     8821 tgatcaaagt cttaaagttg aacaagtaaa tggtgttatg accggcatta aagcacctaa
     8881 aaatataaca aagattcaat tgagctatgc cccaccatac tattatttac ttataacaat
     8941 tactatattt ggcattatat gtagtattat tttcacgaga tgggcaagac aaaaataatg
     9001 tagctacgca tatatttaat gggagtggga cagaaatgat attttcgcaa aatttatttc
     9061 gtcgtcccac cccaacttgc acattattgt aagctgactt tccgccagct tctatgttgg
     9121 ggccccgcca acttgcacat tattgtaagc tgacttttcg tcagcttctg tgttggggcc
     9181 cctgactaga attgaaaaag gcttgttaca agtgcatttt cgttcagtca actactgcca
     9241 atataacttc gtagagatag aacattgatt tatgtcccag ccttttgttt atataaccat
     9301 tttgagcaaa cacgttatga atagagaagg aataaacaat tacattatca actttacatt
     9361 aactaaacag caaaaaaagc catctatatc actccataaa agtgacctag atggctttat
     9421 tgtttgagtc gaatttgact catacatagc tttgtttaaa tgctgattaa cgaatttctt
     9481 ggattctagc agctttacca cgtaaactac gtaagtaata taatttagca cgacgtactt
     9541 taccacgacg tttaacttcg attttttcaa tttttggtgt gtgtaatggg aatgtacgtt
     9601 ccacgccaac acctgatgaa attttacgaa ctgtaaacgt ttcagaaacg ccaccgccac
     9661 gacgtttaat tacaacgcct tcgaatactt ggatacgctc acgagtaccc tcaatgatac
     9721 gtacgtgtac acgtaaagta tcaccaggac ggaaactagg taagtctgta cgtaattgtg
     9781 atttagttac tgcttcgatt aatttgtgat ttgtcattat aaattctcct cttcaaccta
     9841 tgttcttgcc tcgacaaata tatagcagcg gatcatagtg atttttgtgt ttccacactc
     9901 agattatgtt agcacaatac taaccttttt tcaatcctat tttatatctt tctaatattt
     9961 gcttatctgc attagttaat ggatattttt caattaagtc aggtctttta ttatatgtgc
    10021 ggatcaactt ttgctcatgt ctccatgcat caatattggc atgatttcca gacaataaaa
    10081 catctggaac tgttagaccc ttaaattcac gcggacgtgt atattgcgga aactctaata
    10141 acccatctga aaatgaatcg tcttgatgtg actgttcatt acctaaaaca cctggaatca
    10201 gtctaacaat agcatcagtc atggtcatcg ctggcaattc tccaccagtt aaaacatagt
    10261 cacccattga tatttcatct gtgacaagat gtgttcggat acgttcatcg taaccttcat
    10321 aatgtccgca tatgaaaacg atgtggtcgg ccttgcttaa ttcaacagct ttctgatgtg
    10381 aaaatggctc gccttgtgga cacattaaaa taacgcgtgt ttgttctgtg acatctaagt
    10441 cttccatcgc attaaaaaca ggttcaggct ttaacaccat accttgtccg ccaccatacg
    10501 gataatcatc tacttggttg tgcttgttaa ttgcataatc tctaaaatta accgtattga
    10561 tttgtaattt attgttttct tgggcacgtt tcataattga atgatttaaa acaccatcaa
    10621 acatttcagg aaataaagtt aaataatcaa ttttcattaa tccaacaatc cttccattgg
    10681 cgtgatgata atttttttat tttcaacatc cacttctttt acaacatcag caatataagg
    10741 aatcaaatat tctttagatc ctttaatcac ccacacatca ttcgcacctg tttcaaatat
    10801 attaattaca cgacctattg gtgtttcttg atcatcaaaa actgtacatc ctataatatc
    10861 tgaataataa aattcatttt cctcaagtac gatatcttca tgatcacgct cttgataaat
    10921 agaactccct tttaaatgct caatatcatt aatattatta ataccttcaa atgtaagcat
    10981 atgaagccct ttatgcactc tatgtgactt aacagtatat tcaaggtcgt tattgttatg
    11041 cacaactgtc agcacttgac cgggttgaaa acgaacgtct gtaaagtctg aattggattt
    11101 tactttaatt tcacctttaa taccatgtgt gttaacaatt tgaccaactt caactctcat
    11161 tctattacct ccaactctat gctttgaaca cttctatttt aattgaaaat aattcaaaga
    11221 aaaagggcac cattatggta cccaatacgt tctatattac taacatgtga tattcccaac
    11281 gtatcattga gtaataactg tcagtgtaaa cgagttattc ttattgttag aacaatttta
    11341 cgctaaatta cttagctttc ttttgttcgt caaatttttt cataatacct tcttttgata
    11401 agatattgtg aactgtatca gttggtttcg caccatcatt taaccatttt aaagctaacg
    11461 cttcgtcaac tttaatttct ggagcattag cgctcgttgg gttataagta ccgatttgtt
    11521 cgatgatacg tccgtcacgt ggagaacgag catctgctac tacgatacga tagaatggat
    11581 ttctttttga acctaaacgt gttaaacgaa ttttaactgc catttataaa atctccttta
    11641 agtcatgttt taatttttat tttctacaag aaataatatt aacaggtgtg tgaatgtttg
    11701 taaagagttt tttctttacc aggaaaatta ttgaaccttt ttcttacaat aataactatg
    11761 tgattactaa attagactgt taataggtta taaaaattca tagtagcctt caccacatac
    11821 gtgtttagtt aatttatccc gtaaggacat gaatggtacc acttctaccg cacacattcc
    11881 cccaatataa cttattaata ttagcaacaa taacctcatg ttctagcaaa tccgcaattt
    11941 cagtttttaa ttattattgt gtatctaaaa atatgcaccc cataaagtta tattctttag
    12001 tataactttc gggatgcaca tctcattgca acaaaaatca gcgtgttata ttaaacatta
    12061 aaacggtaaa ttcatacctt ttaacatatt ttgcatttga ttgcgtttac cttttttacc
    12121 tttaccgcca ccagtgaatt gtttcatcat tttcttcata tcgttaaatt gtttcatcaa
    12181 acgattgact tcttgtaatg aacgaccaga ccctttagca atacgctttt tacgtgatac
    12241 attcaatgtg tctggattgt ttctttcagc cggcgtcatt gactggataa tcgctttaat
    12301 atgatcaatt tgcttttcac tcatattaag cttatctagc cctttcattt tattcatacc
    12361 tggaatcatt ttcataatat catccagtgg tcctagattt ttcacctgat caagttgttc
    12421 taaaaaatca tctaaagtaa acgatgactc acgcatcttt ttctctaaat cttttgcttt
    12481 ttcttgatcc acatcttgtt gcgctttttc aattaaactt aacacatcac ccatacctaa
    12541 aatacgtgat gccatacgtt caggatggaa tagctctaaa ccatctaact tttcactcat
    12601 accaacaaat ttaattggtt tttgtgtcac cgaacgaata gataaagctg caccaccacg
    12661 tgtatcacca tctaatttag ttaaggtaac acctgtgaca tcaagttgat cgtcaaaaga
    12721 ttctgcaaca ttgacagcat cttgacccgt cattgaatcg acaactaaca taatttcgtt
    12781 tggtttagca atgtctttta cttcttttaa ttcgttcatc aatgcttcat cgatgtgtaa
    12841 tcgacctgct gtatcaatga ttacaaagtc taaatgttct tctttagcat gttttaatgc
    12901 attagttaca atttgttgtg gctttacttg atctccttca ctgtatacag gaatatcaat
    12961 ttgtttccct actgtttgta attgatttat cgctgctgga cgataaatat ctgctgcaac
    13021 taacataggt tttttgttgt attttttacg catcaataat gctaatttac ctgcagttgt
    13081 tgttttacca gcaccttgta aaccaaccat cataacaaca gtaggtggtt tatttgacat
    13141 attaatcgac gtattttctc cacccatcaa ctgcgttaat tcatcttgaa ctattttaat
    13201 aacttgttgc cctggtgtta atgattgcat tacatcggaa cctaatgcgc gttctgatac
    13261 tgttttaata aattctttta ccactttaaa gtttacgtca gcctcaagta acgctaatct
    13321 tacttcacgc atcattatct ttatatcagc ttcagtaagt ttacccttac cacgcatttt
    13381 ttgcatcgtc gcttgcaagc gttctgataa cccttcaaat gccatatcga tgtcctccct
    13441 caatttatat ctattctaag tcttctaatt gttgaatata acgttgtatt tgttctggat
    13501 tacttaaatg ttgtttcatt tcatcatata tttctcggcg ttgctcaaat ttctggtata
    13561 attccaattt cttttcataa tcttcaacta aatcgccagt tcttcttata ttatcataaa
    13621 ctgcttgtct actcacatta aaagtatctg cgatttcact taaagaataa tcttcaagat
    13681 aaaataattc caaataatta cgttgtttat tcgtcaataa ggattgataa aaatcaaaca
    13741 aataattcat tcgtaacgtt ttaactaaat cattttgacc catgattatc gtccttttct
    13801 tctgttacaa tttgatcatt ttcaactgtt gttatttctt cattttgttc aatcatatca
    13861 gcgaataagc cgtagacata actttcaggg ttaaatggtt gtaagtcatc taattgctca
    13921 cctaaaccta catatttaac tggaatgtgc aattcattac gaatggctaa tacgatacca
    13981 cctttggctg taccatctaa tttcgttaat acaatacctg taacatttgt tacttcttta
    14041 aagtttctag cttgtgacaa cgcattctga ccagttgtag catctaaaca tagtaatgct
    14101 tcatgaggcg catctggcac tgctcgatta attacacgct taactttttc taattcttgc
    14161 attagatttg ttttattttg taaacgtcca gcggtatcac agattaaaat atcaacacct
    14221 ttgtttttag cggcattaat cgcatcatac ataacagcag ctggatcaga accttcactt
    14281 tggctaatta cgtctacacc aacacgttcg ccccaaactt tcaattgatc aatagcaccc
    14341 gctctaaaag tatcgcccgc agctaacatt acttttttac cttccatttt atatcggtaa
    14401 gctaattttc caattgttgt tgttttacca acaccattca caccaaccat taaaatgaca
    14461 tttaaacgac catcttctaa gttcatagct tctgaattat catcttcttg atggtaaatc
    14521 tctacgattt tttcaacaat gacttcacgc aaatcttcag tatcttgaat attacgtcgt
    14581 tgtgcttcca tacgtaattc ttcagttaac gtcatcactg tattaaaacc gacgtctgca
    14641 gtgattaaca tttcttctaa agcttcaaaa aagtcttcat ctactttacg atatctcgct
    14701 atcaaattat ttaattgttc ttggaaattt tgacgagatt tttcgagtcc tgctttaaat
    14761 ttagcaccca ttttttgagc ttcaatttct tcaaaatctt caattgatat taagccatcg
    14821 tcatcaaaat ccgcttcact caacttgcgt ggcttctttt tcacttcagc attttctgct
    14881 aaatcatttg cgtcctgcgt tgaactttca gaatgtgtat cttctaattt gtcttgacct
    14941 tgttcttctg ttaaggattt aacttcttca ttttctttat ttgttgcaaa cttatctttt
    15001 aagcgtttaa aaaagctcat tattgctcct ccttcaacac atcatctatt gtatttaaat
    15061 tcacactcac aagtttagta acacctgatt cttgcattgt tacaccgtat aacctatccg
    15121 caaattccat tgttccttta cggtgtgtaa taacaatgaa ttgtgtttcg tctgataact
    15181 catttaaata ttttgcgtat ctaataacat ttgcttcatc tagtgcagct tcaacctcat
    15241 ctaatataac aaaaggtgca gatcttactt ttaaaattgc aaatagtaaa gcaatagcag
    15301 ttaatgcacg ctcaccacca ctcagtaacg ataaatgttg caacttttta cccggtggtt
    15361 gtaccacaat atcaatacca gctgttaaat aatcggcttc agttaattgc aattctgcat
    15421 cgcctccacc aaacaattgt ttgaacacag ctgtaaaatg tccttgaata gcatggaaag
    15481 tttctttaaa tctttcagta acctcttgat ccatttcact tataatttgc tctaatgttt
    15541 ctttagcttt acgaagatct gtacgttgtt cacttaaaaa tgtataacgt tcatttaact
    15601 cttcaaattg ttcaattgca tttaagttta caggacctag ttcatcaatc gacatcttca
    15661 ttaacttaac ttttttacgt aatgcgtcaa tcgattcatc actcgtatat tcagatttcg
    15721 cacgttcaac agtcaattga tattcatcat ttaaatgatc tatcgcatga tgaattaata
    15781 catctagctt tgattgttca gctttaatat cttggtagtg attttcgata gctaaaatat
    15841 cttggtgaca aacttgtagt ttagcttctt gcgcatcgat ttgttcattc aactcaatac
    15901 gttgttgttt caatttatct aattcatcag ataagcgtgt tctcgtttct tgttgaccat
    15961 taatttgatc tttaatattt tgaaaagctt gttcgcccat cacttcatcc gaattaaaca
    16021 atgcaatttt ttctttaaca tcttttaatt gatgtttagt ttgttgattt tgattattta
    16081 atcgatctat tgtctgttgt tgtgttttaa tacgctcttt aaccacagca agatcagatt
    16141 gtttctgatg taacgtttgt tgtgttttag ttacgctttc cttaccttct ttagaaagtt
    16201 ttgtgtagcg ttcaatttca tcttctagtc gttttaaaga tgctttaata ctttctaaat
    16261 gagtttcttt ttcactcaaa gtttgtcgac ttttgtcact cgtataacca tcattttttt
    16321 caaattcgaa ttcttcatga tcatttttta tttgtgtttc ttgtgtagtt aatctatcga
    16381 gctccatttc aaaatgatgc acttgctctt taagtgtatt atgcttttga cttttttcaa
    16441 aatacagttc acttaattga tcacttttta tcttcaactc tttaaattgt tgttcaaatg
    16501 attctgtttg acgcaagtaa tcttctaatt ggtgtctcat tgttgtcaac tcgtcttttt
    16561 gagacagaat acttttggac ttacgagcgc caccaccagt catagaacca ccagggttta
    16621 caatatcacc ttccaaagta acaatacgag ttcgatattt aatcgcacgt gccaattcat
    16681 ttgcatgctt taaatgatca acgataatcg tattacctaa taaattcccg ataatatttt
    16741 gatattctgg tgctacttta actgcttccg aagcaatact aataaatccg tttgcctctt
    16801 tagcaataga tttaatatca gtcgctacca ctctactctg tataacattt aatggtaaaa
    16861 acgtcgcacg acctaaatta cgttctttta aaaattgaat agcctggcgt ccatcttttt
    16921 ctgaatctac aatgacatgt tgtaatgaag cacctaatgc tgtttcaatt gcctgagtta
    16981 atttagatgg cacatcaata atttccgcaa ctgcaccatg aatacccttt aattctttat
    17041 ttttagcttt caaaatatgt ttgacgccat tgaaaaaata agtatattct tcctcttgcg
    17101 ttgccaaact atcaatacgt gttttcattt tttcggtata tcgatatgct tgatacaatt
    17161 tctcttcgta ttcattttgt gctttttttg tatcagtgag atctttttct atatttttaa
    17221 tttctttatc tacagcagaa agttctttgt tggtctgttg atattctttt tttgtcgttt
    17281 ttatttgacc ctgaatatct ttcaattgct caaaaacttc aactaatcga gaatctagtc
    17341 ttgatttttt agcctcattc tcttctatag tatgctttaa aaaacgaata tcattgttaa
    17401 catctgattg ctctgacatt aatgtatagt attcgttttt aatttcttcc aatttttcat
    17461 catgtgcttc gtctgaaaca taaagttgtt cttcaagttc acgaatgaca gcattgagtt
    17521 ctttttgttt acttttcaga gacttataag tatcttgagc tccagaaatc tcatttgata
    17581 tattttctaa aagctccatt aaattttctt gttcttcttc atatcgtgca tttgtttcag
    17641 attgattttt cttacgttct tctaaaacat ttaattgtcc cgtatatttt tcaaaggctt
    17701 ccgtagcttt tactaattga taattaagcg attcaacatc attatcaagt tgatgacgtt
    17761 tacctttata ttgttgaatt tgttggctta aacgttgttt gtcagcttct ttatttgctt
    17821 gttggccttg taaatcattt aaacgttgat ctaattgtct attgtcattt gtatattgat
    17881 caatatcgtg cactgtaact acaatgtcac tatgtttcat ttgatgtgaa agtgtcttat
    17941 attctttagc tatagctgcc tcctctttta gaggttctac acgaccttcc aaatcatata
    18001 aaatgtcttc tactctcgtt aaattatctt ctgtttggtc aagtttattt aatgattcag
    18061 ctttacgttt tttatattta agtacaccag ccgattcttc aataatttga cgtctatcaa
    18121 ttggtttagc atttagtatt tcatcaactc taccttgcga gataatgcta tacgcttctt
    18181 ttcccaatcc agaatctaaa aataaatcgg caatatcttt taatcttgca cggtcattat
    18241 ttatgtagta ctcactttca ccacttcgat acaatcttct tgttacaata acttcgtttt
    18301 catcaacact gagcttttta gaatgattat ctaatcttaa ctgtacttca gcataatttt
    18361 gagctttgcg atgttctgca cctgagaaga taatatcttc catttttgag ccacgtaatg
    18421 atttagccga ttgttcaccc aacacccatt taatagcatc tgtaatatta cttttaccgc
    18481 ttccatttgg accaacaatt gcagttacac ctttatcgaa ttgaacattg gtttgatctg
    18541 caaaagactt aaatccaatg gcatctattg attttaaata aaccatccta aactccttat
    18601 cacaatttta aagaacacat gtatacgata ttttagtatt tgtatattaa atttcgaagt
    18661 tgttccataa aatattaaaa attcttcctt aaatcctaaa tcgaacacct atatgtcgta
    18721 tcatataaat ttctatttaa tttgttttaa ttgcttatag gcactttcag cagcacgttg
    18781 ttctgattct tttttcgttt tccctttacc ttcagctatt gcttcccctt gcagaataac
    18841 ttctgaagtg aatagacgat gatgtgccgg tccctcttct tttattaaat tataggttac
    18901 atcaccttta ttttgctggt gcacatattc ttggaattgt gttttaaaat ctaccacgcc
    18961 taataactca ttttgttcta catgtgggaa aatgactttc tcagcaaatt tccaaactat
    19021 atctagtcct tgatccaaat acaatgcccc aataaatgct tcgaatgcat ctgatattaa
    19081 tgatggtctt gtacgtcccc ctgttttctc ttcaccttta ccaagtaaaa tcatttcgtt
    19141 caatccaatt ttattcgcaa atattacaag tgagggctca catacaatag tggcacgcat
    19201 ttttgttaaa ttcccttctg gcaagttggg atgtttatca aataaatatc gtgaaaccgt
    19261 caattctaat accgcatcac ccaaaaactc taaacgctca ttatggtcta aacgattcat
    19321 attaaaatca ttaataaaac tcgaatgcga aaatgcttgt tggtataaat caatattttg
    19381 ataagtaaag cctaactctg tcattttagt atcaaagcgc tttctaaaac gattaactat
    19441 ctcacttttc ttttgtttag acattatttt atctccttta tgtgttgctc ttgaatcatc
    19501 tttaatttta cgttaaaact attgataatt aaagaaaaaa actgagtcga caatactgac
    19561 gacccagatg taagatttat tatttttcaa gactgttaat aaatttaaca gcatcaccaa
    19621 cagtgttgat tttttcagct tcttcatcag gaatttcagt accaaactcg tcttctaatt
    19681 ccattactaa ttcagcgata tcaagtgagt cagcgcctaa atcatctttg aaagatgcat
    19741 cttcagttac tttatcagcg tctacaccta aacggtcaac gatgatatct tttactttat
    19801 cgaaattttc cacgtcgatt cacctccttt aaaaatgcct atacatagac tattatattt
    19861 tcccatttta aattctaaaa aacaagtaga aattttagaa acacttatca atttattgat
    19921 tagtgtttct tatgcatgag ctttagctca agcgttcctc taatcttctt atcaatttat
    19981 tgattagtgt ttcttaggga gaaattctcg aaccttatct aggttgctta gaatttcccc
    20041 cgcaaagttg actaggtcag gcaattctcc attggatgac cagtcaacca ctgcgtcaat
    20101 tagctttagc tcaggcgttc cgcttatcag ttatcaattt attgattact acgtctttat
    20161 cttgagcttt agctcaaata tattacatgt acattccacc atttacatgg attgtttgac
    20221 ctgtaatata ttttgctttg tctgatgcta agaacgctac tgtattagca atatctgtgt
    20281 cttgaccaaa acgtgctaac ggaatttgag tcaacatttg ttctttaagc tcatcactta
    20341 aagcatctgt catatcagaa acaataaaac caggtgcaac tgcatttaca gtgataccac
    20401 gagatgctaa ttcacgcgcc gcagatttag ttaaaccaat aacacctgct tttgttgcaa
    20461 catagtttgc ttgtcccgga ttacctactg ctccaacaac acttgataaa ttgatgatag
    20521 caccactacg ttgtcttaac atttgtggtg ttgctttttg gatacagtta aatacacctt
    20581 ttaagtttgt gtcaataaca tcatcccact cttgttcttt catacgcatt aataaattat
    20641 cgcgagtaat acctgcatta tttactaaaa catctaaaga accaaattgg ctaactactt
    20701 ctttaatcat tgctttaact tcatcagcat cggcaacatt tgcttgaatc gcaaaactgt
    20761 caacaccttt agctttgatt tcttcgacta ctgcttcagc tttctctttg ctgcctgcat
    20821 agtttactgc tacattatat ccttcttctg ctaattgtaa cgcaatacta cgtccaattc
    20881 ctcttgatgc acctgttact aaagcactct tagtcatttt cattccatcc tttcacatct
    20941 tctaaagttt gaattgatgt taacttaaca tctctattta tttttttaat taagccagat
    21001 aaaacttttc caggaccaat ttcaataaaa tgatcaacac cttggtctat tagccattct
    21061 gttgagttaa tgaattgtac tggtgaatat aattgcttga ccatattaga tttaattact
    21121 tcattgtcag tttcaccttg cgcatttaca ttttgaacta caggaaactt agcatcacgc
    21181 cattcaaatt gattaatata acttgaaaaa tcttcttcaa tcactttcat tagcgatgaa
    21241 tggaatggac cagatactgc taaaggcatg acacgttttg cacctaatga tttacctttt
    21301 tctactagct catcaattaa agctttgtga cctgaaacaa caatttgacc tgggcaatta
    21361 atgtttgctg gttcaattat tttgtcatca gatgataatg acttacaaat ttcatcgact
    21421 ttatcaaaat ctaagcccaa tactgcagcc atacttccta caccagtagg aaatgcttgc
    21481 gccattaatt gaccacgttt tctaacaatt ttaactgcat cttcaaatga taatacgtca
    21541 gctgcaacta aacttgaata ttcacctaaa ctatgcccca tagtaaaatc aggattcaaa
    21601 ttttttagcg ctgctaataa tgccgaacta tgcgtcaata aagctggttg cgtgttttca
    21661 gtttcaccca atttaccttc ttcatcagta aacattgtct ctaaaatatc aaagtctaac
    21721 gtctttgctg ctgaagttaa aatttcagtt gcttgatcat tgttattaaa caaatcttgt
    21781 gccataccaa ctttttgggc accttgtccc ggaaaaataa ttgctgtttt actcatttga
    21841 ttcacctaca gtctctttca ttgtttgtac aatattttgt tctcctgcga ttttcgcttg
    21901 tctaattgca gaataaaaag ctttagcatt tgaactaccg tgtgctttaa ctacagtacc
    21961 ttccaatcct aataatactg aaccaccgta ttctgagtaa tccatctttt tagcgaattc
    22021 tgctaaatct ttcttcaata ttgcacctgc taatttattt ttagtactac tcataatcgt
    22081 atcttttaac attttaccga ttgattttgc agtaccttct aaatttttaa ggaccatgtt
    22141 cccagtatag ccatcggtaa ctacaacatc tgtatcgcca tccattaatg tcttcgcttc
    22201 aatattccca acaaaattca atgaatgatc ttgatttaat aactcatatg attttttcgt
    22261 taaactatta cctttagctg gctcggtacc tatatttaat aatgagattt tcggattatc
    22321 aatacctctg attttttgag cataaatatc ccctagttgc gcatactgta ataagtgttc
    22381 aggtttagca tcagcatttg caccaacgtc taaaaataca aaaccttttc catcaatcgt
    22441 tggcaatgtt actactaaag ccggtctagc tacaccttta atacgtccaa caatgaataa
    22501 accagctgac attaaagcac cagtattacc tgctgacaca catccatctg cttcaccaga
    22561 tttcacagct tcagccattt ttaccattga gctatctttt ttacgtttaa tcgctctaac
    22621 aggctcatct tccatttcaa tcttttcaga acaatgtcta aattcgattc gttcatggtt
    22681 cagattatac tttttttcgt caccgaaaag tataatttct agatctttaa agtcttcaac
    22741 agccttttgt acggcttcta atacgatatc aggcgcattg tcgccaccca tcatatcaat
    22801 tgctaattta accataattt tatcctcgct tatcataaaa cattttaaaa tttcctttga
    22861 aaactaatgt atgtttaaca tatgacttta cttcgacgta ataatgtttt gcagtttgat
    22921 ttacaactcg tgcttctgct cttaccgtat catttaattt tactttttca ataaattgaa
    22981 tgctactctc atgagttaaa actgttggtt gcttaattag cgcaacacat aacgaatttg
    23041 cctgagcaaa cagcacatga ccacgcgcaa ttccagtttt atgaaaaaca gaatccgatg
    23101 taatatctaa aattgattgc gctttaacat ttggattgac ttgaatcaaa tcaccaataa
    23161 attcttgttc ttcaatagaa cttatttggt cataattctt ttcagcaact aatttaatac
    23221 gcttccttaa ttctggtatg tttaaataag tgcgatctaa acgaattgtt tgtatactca
    23281 cttgaaataa gtcgcttagt tcatggtctg tgatgaaggg attgctatca atttgttgtc
    23341 tgattgcttc tctacgttta tctttcttta gtttcaacgt ctcacccctc attttttagt
    23401 acctagtctt aaacattcct cacatatcat aacactttct atttaaccga ctcaacaaat
    23461 aactgtatta tttagctatt atttagtcgg aaatttataa attgttaaaa taccacttta
    23521 catttaaata gttcaagtgt catatcactc aattaacgaa gtgttgcact caaattgaca
    23581 aatcagcatg gtaattagtc aaaactgcga tgtaataaat tttcttcaac aaaatgacgt
    23641 aaatgttgat acgtattttc aaagaatacg ccagattgaa taagttcagc tgcttcatca
    23701 cgagcaactt ctaacatacg ataatcttca actaaattgg caactaagaa atctggcaac
    23761 ccactttgtt taacaccaaa gaaatctcca ggaccacgca tttctaagtc tcgttcactc
    23821 aattcaaatc catccgttgt ttgtgtcata attgtcattc tttcaattcc tgtttctgtt
    23881 ttaggggatg caattaaaac acagtaactt tgctggtcac ttctacctac acgaccgcgt
    23941 aactgatgta aagttgataa tccaaagcga tccgcatcat aaatcatcat aaaagttgca
    24001 ttcggtacat taacacctac ttcaacaaca gtagtagaaa ctaaaacatc tatctcatga
    24061 ttactaaact tttgcatgac ctcatctttt tcatcggcag ataacttacc atgcaataac
    24121 cctacacggg aaacaccata atactgttgt aaagactcgt acaatgcgac aacattttga
    24181 acatcttcga gatgctcaga actttctatt agcgggcaaa tgacatatgc ttgacgacct
    24241 tttttcaact ctgaggtcat ttgcatcaaa actttatcgt attgctcatg ctttgcccaa
    24301 gtagtaatga taggtttacg accttttggt aattgtttaa ttgaagacac atccatctca
    24361 ccaaaaactg atattgctag tgttcttggt atcggggttg ctgtcataaa taacacattc
    24421 gtcattgcac ctttttctct taaaagctgg cgttgattca caccaaatcg atgttgttca
    24481 tctgtaatta ctaaaccaac attatggaaa atcacatcat cttgaatcaa agcatgggtt
    24541 ccaattaaac aatcaatcgt accattttca agttgttcta aaagtatctt tcgtttctta
    24601 ccttttactg acccagttaa caatgcaacg ttcatagaat ctccaaataa agccattaaa
    24661 ctttcagcat gttgctctgc taaaatttca gttggtacca tcaatgctga ttgataacca
    24721 gccgttttta acgcatacat acaaattgca gcaactactg tttttcctga acctacatca
    24781 ccttgaagta atcgatgcat acgtattggt gcttttaaat ctctaaaaat ttcattaaca
    24841 ctggatttct gtgcttcagt tagttcaaaa ggtaaacgat caataaatga tttaacttgg
    24901 tctaagccat aatcaatttc aattgcttcg tcagatgact tttctaatct atttagccat
    24961 tgcatacgta attcgaataa aaacagttca gtaaatgcat aggttctacg agcacgtaat
    25021 aaatcctctt tacttttagg atgatgtaat gtgttcaaag taaagtccaa ggtctctaat
    25081 ttatattttt ctcttagttc atcagttaac cattcatgaa ttgtcacatc atttaacgct
    25141 tgtctaattt ggtctcgtat ttgcttttgt ttaatacctt ccttaatacg atagactggt
    25201 tctaattgaa catctgcgtt ttcttgagtt tgtgtccctt gtgaattaaa gaaaacccta
    25261 ttaccagtaa tttcctgttt aactctattc cacttacctt taacagttat agtttgattt
    25321 aattcgattt tcttttttaa atacggttga ttgaaaaaaa tacatttgac agcaatatta
    25381 tttaccatta aatgaacggt taattttgaa ttatttcttc caaaaaatgc aactactgga
    25441 gctgtatata cttgtccttc tatcgtaacg ttagattgat cttctgcttg attcaaatca
    25501 atcactgtat tatcttcata tctagttggc aaataaagaa caagatcttc cactgtatga
    25561 atatttagtt gttgcaatac ttctattttc ttaggaccta taccttttaa ttgtaaaaga
    25621 gaatatggac tttctattaa gtttacttta gccaaaataa atcacctatg ttttatttga
    25681 ttttacgtat catttagtat gttatttatt ttatcatgac taagttgaac atgacattgt
    25741 tagcaactcg agaatgatac ttttaacccg caataacact aaaatagcaa aataaaaaac
    25801 taccaactga tgatttatca ttggtagttt tttattttaa atttttattc tactgaaaag
    25861 aaatattgat aaattggttg tccaccttca tgaacttcca cttctacatc tggatattgc
    25921 tcttcgatcc agtttatcat gttatctgta actgcttgct ctgcatcttg accaataatc
    25981 acagtcaata tttcactatc ttctgctaac atctcattta acaactcagt aaccgttgtt
    26041 aattgatcac tttggctgct tacaatctta tcttcaatca agcccataaa cgcgtctttt
    26101 ttaatctcaa cgccatcaat tttcgtatca cgaacagcgt acgttaatga accagattta
    26161 acgttattta ctgaatcagc catttgcgct ttattttctt caagtgttgc gtccacatca
    26221 tattggaata gtgcgcttat accttgagga atagatttcg ttggaataac aactgcttct
    26281 gcatcaacaa tactcgctgc ttgttcactt gccattaaaa tatttttatt attcggtaaa
    26341 ataattgcac gtttacattt tgattgttca atgactttaa cgatatcttc tgtagaagga
    26401 ttcatcgttt gtccaccact aatgatatgt gtggcaccca ttgatttgaa tatctctgaa
    26461 ataccttcac ccatagaaat agtaataatt gctgtttcaa ccgtttccat tttcggttta
    26521 gctgtgtgct gttcttttcg aatcacttca cggtgctgtt ctctcatatt ttcaacctta
    26581 agtttaatta attcaccata ttgttgacca taattaaaca ctttacctgg gtattcggta
    26641 tgcacgtgaa ctttcacaat ttcttcatca ttaatgacta ataaagaatc accaaattga
    26701 ctcatatctt gcctgaattc ttgttcatca aaggcttttt tattctttcc aaaacgaacc
    26761 atcatttcag tacaatagcc ataaataata tcttcagtat taattacacc atggaaatca
    26821 tgttcatcat gtacaaattc atctttatct atctttgcaa ctttggcttc aactttttca
    26881 cctttaagcg cttttaagaa tccttcgtaa acgcataaca aacctttacc gccactatca
    26941 acaacaccaa cttctttaag tacagctaat aagtttggtg tgttttcaag tgattcattg
    27001 gcttttacaa taatgtactc cattaattct atacaatctt cagtattatt tgctttttct
    27061 attgcagctt gcgcagcatc ttttgcaact gtaagtattg taccttcaac tggtttcata
    27121 acagctttat atgccgtttc aacaccagct tgaaaacttt cagctaacaa ttttgaatta
    27181 atttcagatt cactttcaat atttttacaa aatcctctga ataattgtga caagatgaca
    27241 ccagagttac ctcttgcacc cattagtaaa cctttcgaga atgttttacc taattcgccg
    27301 atatttttcg acaaattatt ctctacttct tcgcgacctg aagtcatagt aagattcata
    27361 tttgttcctg tatcaccatc tggcactgga tacacattca aagaatctac caaatctgca
    27421 ttgttagata aattttgtgc cccttgtata atcatatcgg caaataattt accattaatt
    27481 ttgctaatca tttcaagttg tcctcctaag ctttcttgcc tgtattattc acacgtacac
    27541 cttgtacata tatattgatt gaatttactg atacattaag tgatttttcc aaagtatatt
    27601 tcactgttga ttgtacatta ttggcaactt cagatatttt cacaccgtaa cttacaataa
    27661 tgtacatatc tatatccact acgccattat tttcagttac tttgatgcct ttagcataat
    27721 tttcatgtcc tagtatttcc gcaataccat ctctaacttg ttgtctagat gccataccta
    27781 caataccata acattcaacg gcctttccac ctacaaccga agcaatcact tcgtttgaaa
    27841 tatcaatttt accgtaatca tttgaaatct ctaatgtcat atctttgtcc ctcctaaatt
    27901 tcaaagttta ttattcgttt tccctaatca cttttaaaat tttaccactt agtgtagtca
    27961 taaacgcttt aagtttcatt gattattttt ataaaatatc aatggtacaa cttatcagaa
    28021 tgacttacgc ttaaatcacc tattatattt tgagtaaact gaatttacac tcatgacgat
    28081 tatctatagg cgttttcttt taatagatgt agtatcatta aataaaaatt ttaaatgttt
    28141 cattcact
//
LOCUS       DAMZLE010000020        27882 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMZLE010000020 DAMZLE010000000
VERSION     DAMZLE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27882
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.020"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            195..27060
                     /gene="ebh"
                     /locus_tag="POB92_000822"
                     /pseudo
     CDS             195..27060
                     /gene="ebh"
                     /locus_tag="POB92_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
     gene            complement(27119..27520)
                     /locus_tag="POB92_000823"
     CDS             complement(27119..27520)
                     /locus_tag="POB92_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDI6578342.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKIIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
BASE COUNT        11205 a         4965 c         5038 g         6674 t
ORIGIN      
        1 aaataaattg ttcaacacat agttgtaatg tgtttcaata ctttttggat agattgcgaa
       61 attgtattga atcgtcatcg ttttaaattt ttaaatgaga atggaatgag cattacaata
      121 tacaagcaat caaaagtaaa tacattcaca acacaacaga gacataacaa caagataagg
      181 agtgaacaat agctgtgaat tatcgtgata aaattcaaaa gtttagtatt cgtaaatata
      241 cagttggtac attttcaact gtcattgcga cattggtatt tttaggattc aatacatcac
      301 aagcacatgc tgctgaaaca aatcaaccag caagcgtggt taaacagaaa caacaaagta
      361 ataatgaaca gactgagaat cgagaatctc aagtacaaaa ttctcaaaat tcacaaaata
      421 gtcaatcatt atctgctact catgaaaatg agcaaccaaa tattagtcaa gctaatttag
      481 tagatcaaaa agtagcgcaa tcatctacta ctaatgatga acaaccagca tctcaaaatg
      541 taaatacaaa gaaagattcg gcaacggctg cgacaacaca accagataaa gaagaaggta
      601 agcataaaca aaacgaaagt caatctgcta ataaaaatgg aaacgacaat agagcggctc
      661 atttagaaaa tcatgaagca aatgtagtaa cagcttcaga ttcatctgat aatggtaacg
      721 tacaacatga ccgaaatgaa ttacaagcat tttttgatgc aaattatcat gattatcgct
      781 ttattgaccg tgaaaatgca gattctggca catttaacta tgtaaaaggc atttttgaaa
      841 agattaatac gttattaggc agtaatgatc caataaacaa taaagacttg caacttgcat
      901 acaaagaatt ggaacaagct gttgctttaa ttcgtacaat gcctcaacgt caacaaacta
      961 gccgtcgatc aaacagaatt caaacgcgtt ctgttgagtc tagagctgca gagcctagat
     1021 cagtatcaga ctatcaaaat gcaaattcat catattatgt tgaaaatgct aatgatggtt
     1081 caggatatcc tgtaggtaca tatatcaatg cttctagtaa aggggcgcca tataatttac
     1141 caactacacc atggaataca ttgaaggcct ctgactcaaa ggaaattgct cttatgacag
     1201 cgaaacaaac tggagatggc taccaatggg ttattaagtt taataaagga catgctccac
     1261 atcaaaatat gattttctgg tttgcattac cagcagacca agtgccagta ggaagaactg
     1321 actttgtaac agttaattca gatggaacaa atgtacaatg gagtcatgga gcagaagcag
     1381 gtgcaaataa accacttcaa caaatgtggg aatatggagt aaatgatcct gatcgttcac
     1441 atgactttaa aataagaaat agaagtggcc aagtaatata tagctggcca actgtccatg
     1501 tttattcttt agaagattta tctagagcga gtgattattt tagtgaagct ggagcaacag
     1561 ctgctactaa agcatttggt agacaaaatt ttgaatatat taatggtcaa aaacctgctg
     1621 aatcaccggg tgttcctaaa gtttatactt tcatcggtca aggtgatgca agttatacaa
     1681 tttcatttaa aacacaaggt ccaactgtta ataaattgta ctatgcagca ggtgggcgtg
     1741 ctttagagta caatcaatta tttatgtaca gtcaactata cgtcgaatca acgcaagacc
     1801 atcaacaacg tcttaatggt ttaagacaag tggttaatcg tacatatcgc ataggtacaa
     1861 ctaaacgtgt agaagtgagt caaggaaatg tacaaacgaa aaaggtatta gaaagtacaa
     1921 acctaaatat agatgatttt gttgatgatc cattaagtta tgttaagacg ccgagtaata
     1981 aagtgttagg tttttaccca actaatgcaa ctactaacgc ttttagaccg gggggcgttc
     2041 aggaattaaa tgaatatcaa ttaagtcaat tatttactga tcaaaaatta caagaagcag
     2101 caagaactag aaacccaata agattaatga ttggtttcga ctatcctgat ggttatggta
     2161 atagtgaaac tttagttcct gttaacttaa cggtattacc tgaaatccaa cataatatta
     2221 aattctttaa aaatgacgat actcaaaaca ttgctgaaaa accattttca aaacaagctg
     2281 ggcatccagt tttctatgta tatgcaggta accaagggaa tgcttccgtg aatttaggtg
     2341 gtagcgtaac atctattcaa ccattacgta ttaatttaac aagtaatgag aattttacag
     2401 ataaagattg gcaaattaca ggtattccgc gtacattaca cattgaaaac tcgacaaata
     2461 gaactaataa tgctagagaa cgtaacattg aacttgttgg taatttatta ccaggggatt
     2521 actttggaac gatacgtttt ggacgtaaag aacaattatt cgaaattcgt gttaaaccac
     2581 atacaccaac aattacaacg acagctgagc aattaagagg tacagcatta caaaaagtgc
     2641 ctgttaatat ttcgggaata ccgttggatc catcggcatt ggtttattta gttgcaccaa
     2701 caaatcaaac tacgaatggt ggtagtgagg cagatcaaat accatctggt tatacgatac
     2761 ttgcgactgg tacacctgat ggggtgcata atacaattac tatacgaccg caagattatg
     2821 ttgtattcat accacctgta ggtaaacaaa ttagagcagt agtttattat aataaagtag
     2881 ttgcatctaa tatgagtaat gctgttacta ttttgccaga tgacattcca ccaacaatca
     2941 ataatcctgt tggaataaat gccaaatact atcgaggcga cgaagtcaac tttacaatgg
     3001 gagtctctga tagacattct ggtataaaaa atacaactat tactactttg ccgaatggtt
     3061 ggacatcaaa tttaactaaa tccgacaaca aaaacggctc attagctatt acaggtagag
     3121 tgagtatgaa tcaggcgttt aacagtgata ttacatttaa agtatcagcg acagataatg
     3181 tcaataatac gacaaatgat agtcaatcta aacatgtgtc aattcatgta ggtaaaatta
     3241 gtgaagatgc tcatccgatt gtattaggaa atactgagaa agttgtagta gtcaatccga
     3301 ctgctgtatc taatgatgaa aagcaaagca taattactgc ctttatgaat aaaaaccaaa
     3361 atataagagg atatttagca tcaactgatc cagtaactgt cgataataat ggtaatgtca
     3421 cattacatta ccgtgatggc tcatcaacaa cgcttgatgc tacaaatgtg atgacatacg
     3481 aaccagttgt gaaatctgaa tatcaaactg ccaatgcagc taaaacagca acggtaacga
     3541 ttgctaaagg acaatcattt aatattggtg atattaaaca atattttact ttaagtaatg
     3601 gacaagctat tccaagtggc acatttacaa atattacatc tgatagaact attctaactg
     3661 cacaagaagt tagtcaaatg aatgcaggca cgcagttata tcatatagtt gcttcaaatg
     3721 catatcataa agacactgaa gatttctata ttagtttaaa aatcgttgat gtgaaacaac
     3781 ctgaaggcga tcaacgtgtc tatcgtacgt caacatatga tttaacaact gatgaaatct
     3841 caaaagtaaa acaagctttt attaatgcaa atagagatgt aattacgctt gccgagggta
     3901 atatttcagt tacaaataca cctaatggtg ctaatgtaag tactattaca gtaaatatta
     3961 ataaaggtcg attaacgaaa tcattcgcgt caaacctagc taatatgaat ttcttgcgtt
     4021 gggttaattt cccgcaagat tatacagtga catggacgaa tgcaaaaatt gcaaacagac
     4081 caacagatgg tggtttatca tggtctgatg accataaatc tttaatttat cgttatgatg
     4141 ctacattagg cacacaaatt acaactaatg atattttaac aatgctaaaa gcgactacta
     4201 cagtgcctgg attgcgtaat aatattactg gtaatgaaaa agcacaagca gaagctggcg
     4261 gaagacctaa ctttagaacg actggttatt cacaatcaaa tgcgacaact gatggtcaac
     4321 gtcaatttac gttgaatggt caagtgattc aagtgttaga catcatcaac ccttcaaacg
     4381 gttatggtgg gcaacctgtt acaaattcaa atactcgtgc aaaccatagt aactcaactg
     4441 ttgttaacgt aaacgaaccg gcagctaatg gtgctagcgc atttacaatt gaccacgttg
     4501 taaaaagtaa ttctacacat aatgcaagtg atgcagttta taaagcacag ttatacttaa
     4561 cgccatatgg tccaaaacaa tatgttgaac atttaaatca aaatacagga aatactactg
     4621 acgctattaa catttatttt gtaccaagtg atttagtgaa tccaacaatt tcagtaggta
     4681 attacactaa tcatcaagtg ttctcaggtg aaacatttac aaatactatt acagcgaatg
     4741 ataactttgg tgtgcaatct gtaactgtac caaatacatc acaaattaca ggcactgttg
     4801 ataataacca tcaacatgtt tctgcaacgg caccaaatgt gacatcagca actaataaga
     4861 caatcaattt attagcaact gatacaagcg gcaatacagc tacaacttcg ttcaatgtaa
     4921 cagtgaaacc tttgcgtgat aaatatcgag ttggtacttc atcaacggct gctaatcctg
     4981 tgagaattgc caatatttcg aataatgcga cagtatcaca agctgatcaa acagcaatta
     5041 ttaattcatt aacgtttact gaaacagtac caaatagaag ttatgcaaga gcaagtgcga
     5101 atgaaatcac tagtaaaaca gttagtaatg tcagtcgtac tggaaataat gccaatgtta
     5161 cagtaactgc tacttatcaa gatggaacaa catcaacagt gactgtacct gtaaagcatg
     5221 tcattccaga aatcgttgca cattcgcatt acactgtaca aggccaagac ttcccagcag
     5281 gtaatggttc tagtgcatca gattacttta agttatctaa tggtagtgcc attccagatg
     5341 caactattac atgggtaagt ggacaagcgc caaataaaga taatacacgt attggtgaag
     5401 atataacagt aactgcacat atcttaattg atggtgaaac aacgccgatt acgaaaacag
     5461 caacatataa agtagtaagt acagtaccga agcatgtctt tgagacgaat agaggtgctg
     5521 tgttcccagg tgtatctgat gtgtatgatg cgaaacaata tgttaaacca gttaatgatt
     5581 cttggacaca aaatgcgcaa cgtatgaatt tccaatttac aaattcatat ggaccaagta
     5641 aagatgtggt tggtatttca acaagggata ttcgagttac ttatgataat caccaaacgc
     5701 aaattatcaa gattttagca aaagttaaac cagatccacc gcgaattgat ggcaactctg
     5761 tgacttataa agcaggtctt actaaccaac aaattaaaat taataatgtg ttaagtagtt
     5821 catctattaa attatttaaa gcagataata cgccattaac gattacaaac acgacttatg
     5881 gtagtggtaa tactgcagtt gtaacggtaa gtgatgcgtt accaaatggt gtgattaagg
     5941 caaggtcttc tatcacaatg aataatgtga cgtacactac tcaagatgaa catggtcgag
     6001 cgatagatgt aacaagaaat gaatctgttg attcaaatga cagtgcaaca gtaacagtga
     6061 caccacaatt acaagcaact actgaaggtg ctgtatttat taaaggtggc gacggttttg
     6121 atttcggaca cgtagaaaga tttattcaaa acccgccaca tggggcaacg gttgcatggc
     6181 atgatagtcc agatacatgg aagaatacag tcggtaacac tcataaaact gcggttgtaa
     6241 cattacctaa tggtcaaggt acgcgtaatg ttgaagttcc agtcaaagtt tatccagttg
     6301 ctaatgcaaa ggcgccatca cgtgatgtga aaggtcaaaa tttgactaat ggaacggatg
     6361 cgatgaacta cattacattt gatccaaata caaacacaaa tggtatcact gcagcatggg
     6421 caaatagaca acaaccaaat aaccaacaag caggcgtgca acatttaaat gtcgatgtca
     6481 catatccagg tatttcagct gctaaacgag ttcctgttac tgttaatgta tatcaatttg
     6541 aattccctca aactacttat acgacaacgg ttggaggcac tttagcaagt ggtacgcaag
     6601 catcaggata tgcacatatg caaaatgcta ctggtttacc aacagatgga tttacgtata
     6661 aatggaatcg tgatactaca ggtacaaatg acgcaaactg gtcagctatg aataaaccga
     6721 atgtggctaa agtcgttaac gcaaaatatg acgtcatcta taacggacat acttttgcaa
     6781 catctttacc agcgaaattt gtagtaaaag atgtgcaacc agcgaaacca actgtgactg
     6841 aaacagcggc aggagcgatt acaattgcac ctggagcaaa ccaaacagtg aatacacatg
     6901 caggtaacgt aacgacatac gctgataaat tagttattaa acgtaatggt aacgttgtga
     6961 cgacatttac acgtcgcaat aatacaagtc catgggtgaa agaagcatct gcagcaactg
     7021 tagcaggtat tgctggaact aataatggta ttactgttgc agcaggtact ttcaaccctg
     7081 ctgatacaat tcaagttgtt gcaacgcaag gaagcggaga gacagtaagt gatgagcaac
     7141 gtagtgatga tttcacagtt gtcgcaccac aaccgaacca agcgactact aagatttggc
     7201 aaaatggtca tattgatatc acgcctaata atccatcagg acatttaatt aatccaactc
     7261 aagcaatgga tattgcttac actgaaaaag tgggtaatgg tgcagaacat agtaagacaa
     7321 ttaatgttgt tcgtggtcaa aataatcaat ggacaattgc gaataagcct gactatgtaa
     7381 cgttagatgc acaaactggt aaagtgacgt tcaatgccaa tactataaaa ccaaattcat
     7441 caatcacaat tactccgaaa gcaggtacag gtcactcagt aagtagtaat ccaagtacat
     7501 taactgcacc ggcagctcat actgtcaaca caactgaaat tgtgaaagat tatggttcaa
     7561 atgtaacagc agctgaaatt aacaatgcag ttcaagttgc taataaacgt actgcaacga
     7621 ttaaaaatgg cacagcaatg cctactaatt tagctggtgg tagcacaacg acgattcctg
     7681 tgacagtaac ttacaatgat ggtagtactg aagaagtaca agagtccatt ttcacaaaag
     7741 cggataaacg tgagttaatc acagctaaaa atcatttaga tgatccagta agcactgaag
     7801 gtaaaaagcc aggtacaatt acgcagtaca ataatgcaat tcataatgcg caacaacaaa
     7861 tcaatactgc aaaaacagaa gcgcaacaag tgattaataa tgatcgtgca acaccacaac
     7921 aagtttctga cgcattaact aaagttcgtg cagcacaaac taagattgat caagctaaag
     7981 cattacttca aaataaagaa gataatagcc aattagtaac atctaaaaat aacttacaaa
     8041 gttctgtgaa ccaagtacca tcaactgctg gtatgacgca acaaagtatt gataactata
     8101 atgcgaagaa gcgtgaagca gaaactgaaa taactgcagc acaacgtgtt atagacaatg
     8161 gagatgcaac accgcagcaa atttcagagg aaaaacatcg tgtcgataac gcattaacag
     8221 cattaaacca agcgaaacaa aatttaacta cagatacaca taccttagag caagcagtgc
     8281 aacaattgaa tcgcacaggt acaacgactg gtaagaaacc agcaagtatt actgcgtata
     8341 ataattcaat gcatgcgctt caagcagaac ttacaagtgc taaaaatagt gctaatgcta
     8401 tcattcagaa gccaataaga tcagtgcaag aggtacaaac agcattaaca aatgtaaatc
     8461 gtgttaatga gcgattaacg caagcaatta atcaattagt acctttagct gataatagtg
     8521 ctttaagaac tgctaagacg aaacttgatg aagaaatcaa taaatcagta actactgatg
     8581 gtatgacaca atcatcaatc caagcatatg aaaatgctaa acgtgcgggt caaacagaat
     8641 caacaaatgc acaaaatgtt attaacaatg gtgacgcaac agaccaacaa attgccgaag
     8701 aaaaaacaaa agtagaagaa aaatataata gcttaaaaca agcaattgct ggattaacac
     8761 cagacttggc accattacaa actgcaaaaa ctcagttgca aaatgatatt gatcagccaa
     8821 cgagtacgac tggtatgaca agcacatcta ttgcagcatt taatgaaaaa ctttcagcag
     8881 ctagaactaa aattcaagaa attgatcgcg tactggcatc tcatccagat gttgcaacga
     8941 ttcgtcaaaa cgtgacagca gcgaatgctg ctaaaacagc acttgatcaa gcacgcaatg
     9001 gcttaacagt tgataaagcg cctttagaaa atgcgaaaaa tcaactacaa catagtattg
     9061 acacgcaaac aagtacaact ggtatgacac aagactctat aaatgcatac aatgcgaagt
     9121 taacagctgc acgtaataag attcaacaaa tcaatcaagt attagcaggt tcaccgactg
     9181 tagaacaaat taatacaaat acgtctgcag caaatcaagc taaatctgat ttagatcatg
     9241 cacgtcaagc gttaacacca gataaagcac cgcttcaaac tgcgaaaacg caattagaac
     9301 aaagcattaa tcaaccaacg gatacaacag gtatgacgac cgcttcgtta aatgcgtaca
     9361 accaaaaatt acaagcagcg cgtcaaaagt taactgaaat taatcaagtg ttgaatggca
     9421 acccaactgt ccaaaatatc aatgataaag tgacagaggc aaaccaagct aaggatcaat
     9481 taaatacagc acgtcaaggt ttaacattag atagacagcc agcgttaaca acattacatg
     9541 gtgcatctaa cttaaaccaa gcacaacaaa ataatttcac gcaacaaatt aatgctgctc
     9601 aaaatcatgc tgctcttgaa acaattaagt ctaacattac ggctttaaat actgcgatga
     9661 cgaaattaaa agacagtgtt gcggataata atacaattaa atcaggtcaa aattacactg
     9721 acgcaacacc agctaataaa caagcgtatg ataatgcagt taatgcggct aaaggtgtca
     9781 ttggagaaac gactaatcca acgatggatg ttaacacagt gaaccaaaaa gcagcatctg
     9841 ttaaatcgac gaaagatgct ttagatggtc aacaaaactt acaacgtgcg aaaacagaag
     9901 caacaaatgc gattacgcat gcaagtgatt taaaccaagc acaaaagaat gcattaacac
     9961 aacaagtgaa tagtgcacaa aatgtacatg cagtaaatga tattaaacaa acgactcaaa
    10021 gcttaaatac tgctatgaca ggtttaaaac gtggcgttgc taatcataat caagtcgtac
    10081 aaagtgataa ttatgtcaac gcagatacta ataagaaaaa tgattacaac aatgcataca
    10141 accatgcgaa tgacattatt aatggtaatg cacaacatcc agttataaca ccaagtgatg
    10201 ttaacaatgc tttatcaaat gtcactagta aagaacatgc attgaatggt gaagctaagt
    10261 taaatgctgc gaaacaagaa gcgaacactg cattaggtca attaaataac ttgaataatg
    10321 cgcaacgtca aaacttacaa tcgcaaatta atagtgcaca tcaaatcgag acagttaata
    10381 cgattaagca aaatgcaaca aacttaaata gtgcaatggg taacttaaga caagctgttg
    10441 cagataaaga tcaagtgaaa cgtacagaag attatgcgga tgcagataca gctaaacaaa
    10501 atgcatataa cagtgcagtt tcaagtgccg aaacaatcat taatcaaaca acaaatccaa
    10561 cgatgtctgt tgatgatgtt aatcgtgcaa cttcagctgt tacttctaat aaaaatgcat
    10621 taaatggtga tgaaaaatta gcacaatcta aaacagatgc tgcaagagca attgatgcat
    10681 taccacattt aaataatgca caaaaagcag atgttaaatc taaaattaat gctgcatcaa
    10741 atattgctgg cgtaaatact gttaaacaac aaggtacaga tttaaataca gcgatgggta
    10801 acttgcaagg tgcaatcaat gatgaacaaa cgacgcttaa tagtcaaaac tatcaagatg
    10861 cgacacctag taagaaaaca gcatacacaa atgcggtaca agctgcgaaa gatattttaa
    10921 ataaatcaaa tggtcaaaat aaaacgaaag atcaagttac tgaagcgatg aatcaattga
    10981 attcggctaa aaataactta gatggtacgc gtttattaga tcaagcgaag caaacagcga
    11041 aacagcagtt aaataatatg acgcatttaa caactgcaca aaaaacgaat ttaacaaacc
    11101 aaattaatag tggtactact gtcgctggtg ttcatacggt tcaatcaaat gccaatacat
    11161 tagatcaagc catgaatacg ttaagacaaa gtattgccaa caaagatgcg actaaagcaa
    11221 gtgaagatta cgtagatgct aataatgata agcaaacagc atataacaac gcagtagctg
    11281 ctgctgaaac gattattaat gcaaatagta atccagaaat gaatccaagt acgattacac
    11341 aaaaagcaga gcaagtgaat agttctaaaa cggcacttaa cggtgatgaa aacttagcta
    11401 cggcaaaaca aaatgcgaaa acgtacttaa acacattaac gagtattaca gatgctcaaa
    11461 agaacaattt gattagtcaa attagtagtg cgacaagagt tagtggtgtt gatactgtaa
    11521 aacaaaatgc acaacatcta gatcaagcta tggctagctt gcaaagtggt attaacaacg
    11581 aatctcaagt taaatcatct gagaaatatc gtgatgctga tacaaataaa caacaagagt
    11641 atgataatgc tattactgca gcgaaagcga ttttaaataa acaacatggt ccaaacactg
    11701 cgcaaaatgc agttgaagca gcattgcaac gtgtgaatac tgcgaaagat gcattgaatg
    11761 gcgatgcaaa attaattgca gctcaaaacg cagcgaaaca acatttaggt actttaacgc
    11821 atatcactac agctcaacgt aatgatttaa caaatcaaat ttcacaagct acaaacttag
    11881 ctggtgttga atctgttaaa caaaatgcga atagtttaga tggtgctatg ggtaacttac
    11941 aaacggctat caacgataag tcaggaacat tagcgagcca aaacttcttg gatgctgatg
    12001 agcaaaaacg taatgcatac aatcaagctg tatcagcagc cgaaaccatt ttaaataaac
    12061 aaactggacc gaatacagcg aaaacagcag tcgaacaagc acttaataat gttaataatg
    12121 cgaaacatgc attaaatggt acgcaaaact taaacaatgc gaaacaagca gcgattacag
    12181 caatcaatgg cgcatctgat ttaaatcaac atcaaaaaga tgcattaaaa gcacaagcta
    12241 atggtgctca acgcgtatct aatgcacaag atgtacaacg taatgcgact gaactgaaca
    12301 cggcgatggg cacattaaaa catgccatcg cagataagac gaatacgtta gcaagcagta
    12361 aatatgtcaa cgccgatagc actaaacaaa atgcttacac aactaaagtt accaatgctg
    12421 aacatattat tagcggtacg ccaacggttg ttacgacacc ttcagaagta acagctgcag
    12481 ctaatcaagt aaacagcgcg aaacaagaat taaatggtga cgaaagatta cgtgtagcaa
    12541 aacaaaacgc caatactgct attgatgcat taacgcaatt aaatactcct caaaaagcta
    12601 aattaaaaga acaagtggga caagccaata gattagaaga cgtacaatct gttcaaacaa
    12661 atggacaagc attgaacaat gcaatgaaag gcttaagaga tagtattgct aacgaaacaa
    12721 cagtcaaagc aagtcaaaac tatacagacg caagtccgaa taaccaatca acatataata
    12781 gcgctgtgtc aaatgcgaaa ggtatcatta atcaaactaa caatccgact atggatgcta
    12841 gtgcgattac ccaagctgca acacaagtga ataatgctaa aaatggttta aacggtgctg
    12901 aaaacttaag aaatgcacaa aacactgcta agcaaaactt aaatacgtta tcacacttaa
    12961 caaataacca aaaatctgcc atctcatcac aaattgaccg tgcaggtcat gtgagtgagg
    13021 taacagctgc taaaaatgca gcaactgagt taaacacgca aatgggcaac ttggaacaag
    13081 ctatccatga tcaaaacaca gttaaacaag gtgttaactt cactgatgca gataaagcta
    13141 aacgtgatgc gtatacaaat gcggtaagca gagctgaagc aattctgaat aaaacgcaag
    13201 gtgcaaatac gtctaaacaa gatgttgaag cggctattca aaatgtttca agtgctaaaa
    13261 atgcattgaa tggtgatcaa aacgttacaa atgcgaagaa tgcagctaaa aatgcattaa
    13321 ataacttaac gtcaattaat aatgcacaaa aacgtgactt aacaactaaa attgatcaag
    13381 caacaactgt agctggtgtt gaagctgtat ctaatacagg tacacaattg aatacagcga
    13441 tggctaactt gcaaaatggt attaatgata aagcaaatac actagcaagt gaaaactatc
    13501 atgatgctga ttcagataag aaaactgctt atactcaagc cgttgcgaac gcagaaaata
    13561 ttttaaataa aaatagtgga tcaaatttag ataaagctgc cgttgaaaac gcgttgtcac
    13621 aagttgctaa tgcgaaaggt gccctaaatg gtaaccataa tttagagcaa gctaaatcaa
    13681 atgcaaacac tactataaac ggacttcaac atttaacaac tgctcaaaaa gataaattga
    13741 aacaacaagt gcaacaagca caaaatgttg caggtgtaga tactgttaaa tcaagtgcca
    13801 acacattaaa tggtgctatg ggtacgttaa gaaatagcat tcaagataac acagctacga
    13861 aaaatggcca aaactatctt gatgctacag gaagtaacaa aacaaactat aacaatgctg
    13921 ttgatagtgc taatggtgtc attaatgcaa caagcaatcc aaatatggat gctaatgcaa
    13981 ttaaccaaat cgctacacaa gtgacatcaa cgaaaaatgc attagatggt acacataatt
    14041 taacgcaagc gaaacaaaca gcaacaaatg ccatcgatgg tgctactaac ttaaataaag
    14101 cgcaaaaaga tgcgttgaaa gcacaagtga caagtgcgca acgtgttgca aatgtaacaa
    14161 gtatccaaca aactgcaaat gaacttaata cagctatggg tcaattacaa catggtattg
    14221 atgatgaaaa tgcaacaaaa caaactcaaa aatatcgtga cgctgaacaa agtaagaaaa
    14281 ctgcttatga tcaagctgta gctgctgcga aagcaatttt aaataaacaa acaggttcaa
    14341 attcagataa agcagcagtt gaccgtgcat tacaacaagt aacaagtacg aaagatgcat
    14401 tgaatggtga tgcaaaactg gcagaagcga aagcggcagc taaacaaaac ttaggcactt
    14461 taaaccatat tacgaatgca caacgtactg cgttagaagg tcaaatcaat caagcgacga
    14521 ctgttgatgg cgttaatact gtaaaaacaa atgccaatac attagacgac gcaatgaata
    14581 gcttacaagg tgcaatcaat gataaagatg ctacattaag aaatcaaaat tatcttgatg
    14641 cagatgaatc aaaacgaaat gcatatacgc aagctgtcac agcggctgaa ggcattttaa
    14701 ataaacaaac tggtggtaac acatctaaag cagacgttga taatgcatta aatgcagtta
    14761 caagagcgaa agcggcttta aatggtgctg aaaacttaag aaatgcgaaa acttcagcaa
    14821 caaatacgat taatggttta cctaacttaa cacaattaca aaaagacaac ttgaagcatc
    14881 aagttgaaca agcgcaaaat gtagcaggtg taaatggtgt taaagataaa ggtaatacct
    14941 taaatactgc catgggtgca ttacgtacaa gtatccaaaa tgataatacg acgaaaacaa
    15001 gtcaaaatta tcttgatgca tctgatagca acaaaaataa ttacaatact gctgtaaata
    15061 atgcaaatgg tgttattaat gcaacgaaca atccaaatat ggatgctaat gcgattaatg
    15121 gcatggcaaa tcaagtcaat acaacaaaag cagcgttaaa tggtgtacaa aacttagctc
    15181 aagctaaaac aaatgcgacg aacacaatta acaacgcaca tgacttaaac caaaaacaaa
    15241 aagatgcatt aaaaacacaa gttaacaatg cacaacgtgt atctgatgca aataacgttc
    15301 aacatacagc tactgaattg aacggtgcga tgacagcact taaagcagct attgctgata
    15361 aagaaagaac aaaagcaagc ggtaattatg tcaatgctga tcaagaaaaa cgtcaagcgt
    15421 atgattcaaa agtgactaac gctgaaaata tcattaatgg tacaccgaat gcgacattaa
    15481 cagtcaatga tgtaaatagt gcgacttcac aagtcaatgc ggctaaaaca gcattaaatg
    15541 gcgataacaa cttacgtgtt gctaaagaaa atgctaataa tacaattgat ggcttagcac
    15601 aattgaataa tgcacaaaaa gcaaaattaa aagaacaagt tcaaagtgca actacattag
    15661 aaggtgttca aactgttaaa aatagttctc aaacgttgaa tacagcgatg aaaggcttaa
    15721 gagatagtat tgcgaatgaa gcaacaatta aagcaggtca aaactacact gacgcaagtc
    15781 caactaatcg taacgagtac gacagcgcag ttactgcagc aaaagcaatc attaatcaaa
    15841 catcgaaccc aacgatggaa ccaaatacta ttacgcaagc aacatcacaa gtgacaacta
    15901 aagaacatgc attaaatggt gcgcaaaact tagctcaagc taagacaact gcgaaaaaca
    15961 acttaaataa cttaacatca attaacaatg cacaaaaaga tgcgttaacg cgtagcattg
    16021 atggtgcaac tacagtagct ggtgtaaatc aagaaactgc caaagcaaca gaattaaata
    16081 acgcaatgca tagtttacaa aatggtatca atgatgagac acaaacaaaa caaactcaga
    16141 aatacctaga tgcagagcca aataagaaat cagcttatga tcaagctgta aatgcagcga
    16201 aagcaatttt aacaaaagct agtggtcaaa atgtagacaa agcagcagtt gaacaagcat
    16261 tacaaaatgt gaacagtacg aagacggcgt tgaacggtga tgcgaaatta aatgaagcta
    16321 aagctgctgc gaaacaaacg ttaggtacat taacacacat taataatgca caacgtaatg
    16381 cgttagataa tgaaattaca caagcaacaa atgttgaagg tgttaataca gttaaagcca
    16441 aagcgcaaca attagatggt gctatgggtc aattagaaac atcaattcgt gataaagaca
    16501 cgacgttaca aagtcaaaat tatcaagatg ctgatgatgc taaacgaacg gcttattctc
    16561 aagcagtaaa tgcagcagca actattttaa ataaaacagc tggcggtaat acacctaaag
    16621 cagatgttga aagagcaatg caagctgttg cacaagcaaa tactgcatta aacggtattc
    16681 aaaacttaga acgtgcgaaa caggctgcta acacagcgat tacaaacgct tcggacttaa
    16741 atacaaaaca aaaagaagca ttaaaagcac aagtaacaag tgcaggacgc gtatctgcag
    16801 caaatggtgt tgaacatact gcgactgaaa taaatactgc gatgacagct ttaaaacgtg
    16861 ctattgctga taaagctgac acaaaaacta gtggtaacta tgtcaatgct gatgcgaata
    16921 aacgtcaagc atatgatgaa aaagtgacag ctgccgaaag tatcgttaat ggtacaccaa
    16981 caccaacgtt aacaccatca gatgttacaa atgcagcaac gcaagtaacg aatgcgaaga
    17041 cgcagttaaa cggtaatcat aatttagaag tagcgaaaca aaatgctaac acagcaattg
    17101 atggtttaac ttctttaaat ggtccgcaaa aagcaaaact taaagaacaa gtgggtcaag
    17161 cgacgacgtt gccaaatgtt caaactgttc gtgataatgc acaaacatta aacactgcaa
    17221 tgaaaggtct acgagatagc attgcgaatg aagcaacgat taaagcaggt caaaactaca
    17281 cagatgcaag tcctaataat cgtagtgaat acgatagtgc agtcactgca gcaaaagcaa
    17341 tcattggtca aacaactagt ccatcaatga atgcgcaaga aattaatcaa gcgaaagacc
    17401 aagtgacagc taaacaacaa gcgttaaacg gtcaagaaaa cttaagaact gcgcaaacaa
    17461 atgcgaagca acatttgaat ggcttaagtg acttaactaa tgctcaaaaa gaagcggcga
    17521 aacgtcaaat cgagggtgca acgcatgtta atgaagtaac acaagcacaa aataatgcgg
    17581 atgcattaaa tacagctatg acgaacttga aaaatggtat tcaagatcag aatacgatta
    17641 agcaaggtgt taacttcact gatgcagatg aagcgaaacg taatgcatat acaaatgcag
    17701 tgacgcaagc tgaacaaatt ttaaataaag cacaaggtcc aaatactgca aaagacaatg
    17761 ttgaatctgc gttacaaaat gtacaacgtg ctaaaaacga attgaacggt aatcaaaatg
    17821 ttgcgaatgc taagacaact gcgaaaaatg cattgaataa cttaacatca atcaacaacg
    17881 cacaaaaaga agcgttgaaa tcacaaattg aaggtgctac aacagttgca ggtgtaaatc
    17941 aagtgtctac aacagcatct gaattaaata ctgcaatgag caacttacaa cgtggtatta
    18001 acgatgaagc agctacaaaa gcagctcaga aatatactga tgcagataga gataaacaaa
    18061 ctgcatacaa tgatgctgta acggcagcta aaacgttatt agataaaaca gctggcacaa
    18121 atgaaaataa agcagccgtt gaacaagcat tacaacgtgt gaatactgct aaaactgcat
    18181 taaatggtga tgcgcgctta aatgaagcga agaacacagc taaacaacaa gtagcgacaa
    18241 tgtcacactt aactgatgct caaaaagcaa acttaacatc gcaaatcgaa agtggtacga
    18301 ctgttgcagg tgttcaaggt attcaagcta atgccggtac tttagatcaa gcaatgaatc
    18361 aattaagaca aagtattgct tctaaagatg cgactaaatc aagcgaagat tatcaaaacg
    18421 cgaatgcaga tttacaaaat gcatacaatg atgcggtaac taatgctgaa ggtattatta
    18481 gtgcaacgaa taaccctgaa atgaatcctg atacaattaa ccaaaaagcg agccaagtga
    18541 acagtgcgaa gtctgcattg aacggtgatg aaaaattagc agcagcaaaa caaactgcga
    18601 aatcagatat cggtcgtttg acagacttga acaatgcaca acgaactgcg gcaaatgctg
    18661 aagtggatca agcaccaaat cttgcagctg ttacagcggc taaaaataaa gcaacatcgt
    18721 taaacacagc gatgggtaat ttgaaacatg cacttgctga aaaggataat acgaaacgta
    18781 gtgtcaatta cacagatgcg gatcaaccaa aacaacaagc gtatgatact gcggttacac
    18841 aagcagaagc aattactaat gctaatggca gcaacgcgaa tgaaacaaca gttcaagcag
    18901 cactaaacca attgaatcaa gctaaaaatg acttgaatgg tgataataaa gttgctcaag
    18961 caaaagagtc agcgaaacgt gcattagctt catatagtaa cttgaataat gcgcaatcaa
    19021 ctgcagcaac tagtcaaatt gacaatgcaa cgacagtagc aggtgtaact gctgcacaaa
    19081 atactgctaa tgaattaaat acagcaatgg gtcaacttca aaatggtatt aatgaccaaa
    19141 acactgttaa acaacaagtg aactttacag atgctgacca aggtaagaaa gatgcttaca
    19201 caaatgctgt tacgaatgct caaggtattt tagataaagc aaacggtcaa aatatgacaa
    19261 aagcacaagt tgaagctgca ttaaatcaag taacgactgc taagaatgct ttaaacggtg
    19321 atgcaaatgt aagacaagca aaatcagatg cgaaagcaaa tttaggtaca ttaacacact
    19381 taaataatgc acaaaaacaa gatttaacat cacaaatcga aggtgcaaca acagttaacg
    19441 gtgtaaatag tgttaaaacg aaagcacaag acttagatgg tgcaatgcaa cgattagagt
    19501 cagcaatcgc aaataaagat caaactaaag cgagcgaaaa ctacatcgac gcagatccaa
    19561 ctaagaaaac agcatttgat aatgctatca cacaagctga atcttactta aataaagatc
    19621 atggtacgaa taaagataag caagctgttg aacaagcaat tcaaagtgta acgtctactg
    19681 aaaatgcttt gaacggtgac gcgaacttac aacgcgctaa aactgaagca acacaagcta
    19741 tcgataactt gacacatttg aatacaccac aaaaaacagc attaaaacaa caagtgaacg
    19801 ctgcgcaacg tgtatcaggt gtaactgatc tgaaaaatag tgctacatca cttgataatg
    19861 cgatggatca attaaaacaa ggaattgctg atcatgacac aattgttgct ggtggtaatt
    19921 acactaacgc aagtcctgat aagcaaggtg cttatactga tgcatataat gctgcgaaaa
    19981 acattgtaaa tggttcacct aatgtgatta caaatgcagc agatgttact gcggcaacac
    20041 aacgtgtcaa taatgctgaa acaagtttaa atggtgattc aaacttagca actgcgaagc
    20101 aacaagctaa agatgcatta cgtcaaatga cacatttatc tgatgcacaa aaacaaagta
    20161 ttactggtca aattgatagc gcgacacaag taactggtgt acaaagtgtg aaagacaatg
    20221 caacaaatct tgacaatgca atgaatcaac ttcgaaatag tattgcgaat aaagatgaag
    20281 taaaagcgag tcaaccatat gttgatgcag atacagataa acaaaatgca tacaatacag
    20341 cagttacaag tgctgaaaat atcattaatg caacgagtca gccaacactt gatccatctg
    20401 cagtaacaca agcagctaat caagtgaaca ctaacaaaac tgcgcttaat ggtgcgcaaa
    20461 acttagcaaa taaaaagcaa gaaacaactg ctaacatcaa ccaattaagt catttaaata
    20521 atgctcaaaa gcaagattta aatacacaag tgacaaatgc accaaatatt agcacagtaa
    20581 atcaagtgaa aactaaagct gaacaattag atcaagcaat ggaacgttta atcaacggaa
    20641 tccaagacaa agatcaagtg aaacaaagtg ttaactttac agatgcagat ccagaaaaac
    20701 aaacagcata caacaatgcg gtaactgctg ctgaaaatat tattaatcaa gcaaatggta
    20761 caaatgcgaa ccaatcacaa gttgaagcag cactttcaac tgtaacaact actaaacaag
    20821 cgttgaatgg tgatagaaaa gtaacagatg ctaaaaacaa tgcaaaccaa acattatcta
    20881 cgttagataa cttaaacaat gcacaaaaag gtgctgttac tggaaacatc aatcaagcgc
    20941 acactgtagc tgaagtaacg caagccattc aaaccgctca ggaactgaat acagcgatgg
    21001 gtaacttgaa aaatagcttg aatgataaag acactacact tggcagtcaa aactttgcag
    21061 atgcagatcc agagaagaaa aatgcataca atgaagcggt tcgtaatgct gaaaatattt
    21121 taaataaatc tacaggtaca aacgtgccta aagatcaagt tgaagcagct atgaatcaag
    21181 tgaatactac aaaagcagcg cttaatggta ctcaaaacct tgaaaaagct aaacaacacg
    21241 caaatacagc aattgacggt ttaagccatt taacaaatgc acaaaaagag gcattaaaac
    21301 aattggtaca acaatcgact actgttgcag aagcacaagg taatgaacaa aaagcaaaca
    21361 atgttgatgc agcaatggac aaattacgtc aaagtattgc agataatgcg acaacaaaac
    21421 aaaaccaaaa ttatactgat taataatgca acaactcgtg ataaagtggc tgaaatcatt
    21481 gcacaagcgc aagcattaaa tgaagcgata aaagcattaa aagaaagtat taaggatcaa
    21541 ccacaaactg aagcaagtag taaatttatt aacgaggatc aagcgcaaaa agatgcttat
    21601 acgcaagcag tacaacacgc gaaagatttg attaacaaaa caactgatcc tacattagcc
    21661 aaatcaatca ttgatcaagc gacacaggca gtgactgatg ctaaaaacaa tttacatggt
    21721 gatcaaaaac tagctcaaga taagcaacgt gcaacagaaa cgttaaataa cttgtctaac
    21781 ttgaatacac cacaacgtca agcacttgaa aatcaaatca ataatgcagc aactcgtgtc
    21841 gaagtagcac aaaaattaac tgaagcacaa gcacttaacc aagcaatgga agctttacgt
    21901 aatagcattc aagatcaaca gcaaacggaa gcgggtagca agtttatcaa tgaagataaa
    21961 ccacaaaaag atgcttacca agcagcagtt caaaatgcaa aagatttaat taatcaaact
    22021 aacaatccaa cgcttgataa agcacaagtt gaacaattga cacaagctgt taaccaagct
    22081 aaagataacc tacacggtga tcaaaaactt gcagacgata aacaacatgc ggttactgat
    22141 ttaaatcaat taaatggttt gaataatccg caacgtcaag cacttgaaag ccaaataaac
    22201 aacgcagcaa ctcgtgatga agtagcgcaa aaattagctg aagcaaaagc gcttgatcaa
    22261 gcaatgcaag cattacgtaa tagtattcaa gatcaacaac aaacagaatc tggtagcaag
    22321 tttatcaatg aagataaacc gcaaaaagat gcttaccaag cagcagttca aaatgcaaaa
    22381 gatttaatta accaaacagg taatccaacg cttgataaag cacaagttga acaattgaca
    22441 caagcagtaa caactgcaaa agataaccta catggtgatc aaaaacttgc tcgtgatcaa
    22501 caacaagcag taacaactgt aaatgcattg ccaaacataa atcatgcaca acaacaagca
    22561 ttaactgatg ctataaatgc agcgcctaca agaacagagg ttgcacaaca tgttcaaact
    22621 gctactgaac ttgatcacgc gatggaaaca ttgaaaaata aagttgatca agtaaataca
    22681 gataaggctc aaccaaatta cactgaagcg tcaactgata aaaaagaagc agtagaccaa
    22741 gcgttacaag ctgcagaaag cattacagat ccaactaatg gttcaaatgc gaataaagac
    22801 gctgtagaac aagcattaac taagcttcaa gaaaaagtaa atgagttaaa tggtgatgag
    22861 agagtcgctg aagctaaaac acaagcgaaa caaaatattg accaattaac acatttaaat
    22921 gctgatcaaa tcgcaactgc taaacaaaac attgatcaag cgacgcaact tcaaccaatc
    22981 gctgaattag tagatcaagc aacgcaattg aaccaatcaa tggatcaatt acaacaagct
    23041 gttaatgatc atacaaatgt tgagcaaaca gttgattaca cgcaagcaga ttcagataag
    23101 caaaaggctt ataaacaagc gattgctgat gctgaaaatg tattgaaaca aaatgcgaat
    23161 aagcaacaag tggatcaagc acttcaaaat attttaaatg caaaacaagc attaaatggt
    23221 gatgaacgtg tagcacttgc taaaacaaat ggtaaacatg acatcgacca attgaatgca
    23281 ttaaacaatg ctcaacaaga tggatttaaa ggtcgcatcg atcaatcaaa cgatttaaat
    23341 caaatccaac aaattgtaga tgaggctaag gcacttaatc gtgcaatgga tcaattgtca
    23401 gaagaaatca ctggcaatga aggacgcacg aaaggtagca cgaactatgt caatgcagat
    23461 acacaagtca aacaagtata tgatgaagcg gttgataaag cgaaacaagc acttgataaa
    23521 tcgactggtc aaaacttaac tgcagaacaa gttatcaaat taaatgatgc agtaacggca
    23581 gcgaaacagg cattaaatgg tgaagaaaga cttaataatc gtaagtctga agcattacaa
    23641 agattggatc aattaacaca tctaaacaat gctcaaagac aattagcaat ccaacaaatt
    23701 aataatgctg aaacgctaaa taaagcatct cgagcaatta atagagcaac taaattagat
    23761 aatgcaatgg gtgcagtaca acaatatatt gacgaacagc accttggtgt tatcagcagc
    23821 acaaattaca tcaatgcaga tgacaatttg aaagcaaatt atgataatgc aattgcgaat
    23881 gcagcacatg agttagataa agtgcaaggt aatgcaattg caaaagctga agcagagcaa
    23941 ttgaaacaaa atattatcga tgctcaaaat gcattaaatg gagaccaaaa ccttgcaaat
    24001 gccaaagata aagcaaatgc gtttgttaat tcgttaaatg gattaaatca acagcaacaa
    24061 gatcttgcac ataaagcaat taacaatgcc gatactgtat cagatgtaac agatattgtt
    24121 aataatcaaa ttgacttaaa tgatgcaatg gaaacattga aacatttagt tgacaatgaa
    24181 attccaaatg cagagcaaac tgtcaattac caaaacgctg acgataatgc taaaacaaac
    24241 ttcgatgatg ccaaacgtct agcaaatgca ttgctaaata gtgataacac aaatgtgaat
    24301 gatatcaatg gcgcaatcca agcagtcaat gatgcaatcc ataatcttaa tggtgatcaa
    24361 cgactacaag atgctaaaga caaggcaatt caatctatta atcaagcttt agctaataag
    24421 ctaaaagaaa tcgaagcttc aaatgcgacg gatcaagaca agcttattgc gaaaaataaa
    24481 gcagaagaat tggcaaacag catcatcaac aacattaata aagcaacaag taatcaggat
    24541 gtatctcaag ttcaaacagc tggcaaccat gcgattgaac aagtgcatgc caatgaaata
    24601 ccgaaagcta aaattgatgc gaataaagat gttgacaagc aagttcaagc attaattgac
    24661 gaaattgatc gaaatccaaa tctaacagat aaggaaaaac aagcacttaa agatcgtatt
    24721 aatcaaatac ttcaacaagg tcataacgac attaacaatg cgctgactaa agaagaaatt
    24781 gaacaagcca aagcacaact tgcgcaagca ttacaagaaa ttaaagattt agtgaaagct
    24841 aaagaaaatg cgaaacaaga tgttgacaag caagttcaag ctttaattga cgaaatcgat
    24901 caaaatccaa atctaacaga taaggaaaaa caagcactta aagatcgaat taaccaaata
    24961 cttcaacaag gtcataacga cattaacaat gcgatgacta aagaagaaat tgaacaagcc
    25021 aaagcacaac ttgcacaagc attacaagac atcaaagatt tagtgaaagc taaagaagat
    25081 gcgaaaaatg caataaaagc cttagctaat gcgaagcgtg atcaaatcaa ttcaaatcca
    25141 gatttaacac ctgagcaaaa agcaaaagcg ctcaaagaaa ttgacgaagc tgaaaaacga
    25201 gcactacaaa acgttgagaa tgctcaaact atagatcaat taaatcgagg attaaactta
    25261 ggtttagatg acattagaaa tacacatgta tgggaggttg atgaacaacc tgctgtaaat
    25321 gaaatttttg aagcaacacc tgagcaaatc ctagttaatg gtgaactcat tgtacatcgt
    25381 gatgacatca ttacagaaca agatattctt gcacacataa acttaattga tcagctttca
    25441 gcagaagtta ttgatacacc atcaactgca acgatttctg atagcttaac agcaaaagtt
    25501 gaagttacat tgcttgatgg atcaaaagtg attgttaatg ttcctgtaaa agttgtagaa
    25561 aaagaattgt cagtagtcaa acaacaggca attgaatcaa tcgaaaatgc ggcacaacaa
    25621 aagattaatg aaatcaataa tagtgtgaca ttaacactgg aacaaaaaga agctgcaatt
    25681 gcagaagtta ataagcttaa acaacaagca attgatcata ttaacaatgc acctgatgtt
    25741 cattcagttg aagaaattca acaacaagaa caagcgcata ttgaacaatt taatccagaa
    25801 caatttacga ttgaacaagc aaaatcaaat gcaattaaat cgattgaaga tgcaattcaa
    25861 catatgattg atgaaatcaa agctcgtact gatctaacag ataaagagaa gcaagaagct
    25921 attgctaagt taaatcaatt aaaagaacaa gcaattcaag cgattcaacg tgcgcaaagc
    25981 atcgatgaaa taactgagca attggaacaa tttaaagctc aaatgaaagc agctaatcca
    26041 acagcaaaag aactagctaa acgcaagcaa gaagctatta gtagaattaa agacttttca
    26101 aatgaaaaaa tgaatagtat tcgaaatagt gaaattggca cagctgatga aaaacaagca
    26161 gcaatgaatc aaattaacga aattgtgctt gaaacaatta gagatattaa taatgcgcat
    26221 acattacagc aagttgaggc tgcattgaac aatggtattg ctcgaatttc agcagtacaa
    26281 attgtaatat ctgatcgtgc taaacaatct tcaagtactg gaaatgaatc taatagccat
    26341 ttaacaattg gttatggaac tgcaaatcat ccatttaaca gttcgactat tggacataaa
    26401 aagaaaattg atgaagatga tgacattgat ccacttcata tgcgtcactt tagtaataat
    26461 ttcggtaatg ttattaaaaa cgctattggt gtggtgggta tctctggctt actagctagt
    26521 ttctggttct tcattgccaa acgtcgtcgt aaagaagatg aagaggaaga attagaaata
    26581 agagataata ataaagattc aataaaagag actttagacg atacaaaaca tttaccactt
    26641 ttatttgcga aacgtcgcag aaaagaagat gaagaagatg ttactgttga agaaaaagat
    26701 tcgctaaata atggcgagtc actcgataaa gttaaacata cgcctttctt cttaccaaaa
    26761 cgtcgtcgta aagaagatga agaagatgtg gaagttacaa atgaaaacac agatgaaaaa
    26821 gtgttgaaag ataacgaaca ttcaccactc ttattcgcaa aacgacgcaa agataaagag
    26881 gaagatgttg aaacaacaac tagtattgaa tctaaagatg aggacgttcc tttattattg
    26941 gctaaaaaga aaaatcaaaa agataaccaa tccaaagaca aaaagtcagc atcaaaaaat
    27001 acttctaaaa aggtagcagc caaaaagaag aaaaagaaat ctaagaaaaa taaaaaataa
    27061 tttgtttctt tgataaatag aggagcaccg attgacatca catcagtcgg tgctcctttt
    27121 atttattctt tttaattaat ttatacaatg cctgttgagc gtgttgattc gcttctttgt
    27181 tttgttctct cggtatccat ttaacaaata ataaatcaaa atctttttca aatatttcta
    27241 tttgatcaaa ataaggtttg aaatttgcgt ttttcacata accagcttca atgctatctg
    27301 caattatctt tgagtctgta tataatagtg cgttttgaac atttaattca cgtgcatgtt
    27361 ctagtgcata aatacatgca gcccattctg cagtgtggtt atccatttcg cctaactcat
    27421 gtgtatatgt ataatgctgc tcatcttctt tgattacaat ggcacatgta cttatgcctg
    27481 gatttccttt cgtcgcagca tcaaaattta ttttcgccat aataaaccta ctttctattc
    27541 aatacttagt taaagttact attactgtaa tacaaaatat gttgggtaat ccattaaaaa
    27601 acacgcatca cttaaataag taacacgtgt ttaaaatact cgctgattca aagatgattt
    27661 tctaatacgt agtactgtaa tatacttcct aaaaaatcat cttcaggctg ggacataaat
    27721 caatgttcta tgctctacga tgttatattg gcagtagttg actgaacaaa aatgcgcttg
    27781 taacaagctt ttttcaattc tagtcagggg ccccaacaca gagaatttcg aaaagaaatt
    27841 ctacaggcaa tgcgagttgg ggtgtgggcc ccaacacaga ag
//
LOCUS       DAMZLE010000021        27395 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMZLE010000021 DAMZLE010000000
VERSION     DAMZLE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.021"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(93..503)
                     /locus_tag="POB92_000824"
     CDS             complement(93..503)
                     /locus_tag="POB92_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI6578343.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(490..1353)
                     /locus_tag="POB92_000825"
     CDS             complement(490..1353)
                     /locus_tag="POB92_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI6578344.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(1395..2180)
                     /locus_tag="POB92_000826"
     CDS             complement(1395..2180)
                     /locus_tag="POB92_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI6578345.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(2306..3196)
                     /locus_tag="POB92_000827"
     CDS             complement(2306..3196)
                     /locus_tag="POB92_000827"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI6578346.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(3206..4552)
                     /locus_tag="POB92_000828"
     CDS             complement(3206..4552)
                     /locus_tag="POB92_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6578347.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(4666..5766)
                     /locus_tag="POB92_000829"
     CDS             complement(4666..5766)
                     /locus_tag="POB92_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDI6578348.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDVYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNRFNTEKINIDVEASTINTDPFQYI"
     gene            complement(5769..6446)
                     /locus_tag="POB92_000830"
     CDS             complement(5769..6446)
                     /locus_tag="POB92_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDI6578349.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDGLKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(6577..7683)
                     /gene="rpoD"
                     /locus_tag="POB92_000831"
     CDS             complement(6577..7683)
                     /gene="rpoD"
                     /locus_tag="POB92_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI6578350.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(7907..9724)
                     /locus_tag="POB92_000832"
     CDS             complement(7907..9724)
                     /locus_tag="POB92_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6578351.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQF
                     NSNIQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTLNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPDDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(9785..10603)
                     /locus_tag="POB92_000833"
     CDS             complement(9785..10603)
                     /locus_tag="POB92_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDI6578352.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(10614..11237)
                     /locus_tag="POB92_000834"
     CDS             complement(10614..11237)
                     /locus_tag="POB92_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6578353.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            11572..12963
                     /locus_tag="POB92_000835"
     CDS             11572..12963
                     /locus_tag="POB92_000835"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDI6578354.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(13114..13860)
                     /gene="recO"
                     /locus_tag="POB92_000836"
     CDS             complement(13114..13860)
                     /gene="recO"
                     /locus_tag="POB92_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI6578355.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLFVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(13888..14787)
                     /gene="era"
                     /locus_tag="POB92_000837"
     CDS             complement(13888..14787)
                     /gene="era"
                     /locus_tag="POB92_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI6578356.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(14788..15192)
                     /gene="cdd"
                     /locus_tag="POB92_000838"
     CDS             complement(14788..15192)
                     /gene="cdd"
                     /locus_tag="POB92_000838"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI6578357.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(15203..15547)
                     /locus_tag="POB92_000839"
     CDS             complement(15203..15547)
                     /locus_tag="POB92_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6578358.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(15550..16017)
                     /gene="ybeY"
                     /locus_tag="POB92_000840"
     CDS             complement(15550..16017)
                     /gene="ybeY"
                     /locus_tag="POB92_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI6578359.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRG"
     gene            complement(16018..16965)
                     /locus_tag="POB92_000841"
     CDS             complement(16018..16965)
                     /locus_tag="POB92_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI6578360.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNMTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(17269..17967)
                     /locus_tag="POB92_000842"
     CDS             complement(17269..17967)
                     /locus_tag="POB92_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDI6578361.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(17984..18973)
                     /gene="floA"
                     /locus_tag="POB92_000843"
     CDS             complement(17984..18973)
                     /gene="floA"
                     /locus_tag="POB92_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDI6578362.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(18991..19674)
                     /locus_tag="POB92_000844"
     CDS             complement(18991..19674)
                     /locus_tag="POB92_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI6578363.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(19918..20094)
                     /locus_tag="POB92_000845"
     CDS             complement(19918..20094)
                     /locus_tag="POB92_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI6578364.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(20387..21733)
                     /gene="mtaB"
                     /locus_tag="POB92_000846"
     CDS             complement(20387..21733)
                     /gene="mtaB"
                     /locus_tag="POB92_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDI6578365.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(21740..22492)
                     /locus_tag="POB92_000847"
     CDS             complement(21740..22492)
                     /locus_tag="POB92_000847"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI6578366.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(22494..23432)
                     /gene="prmA"
                     /locus_tag="POB92_000848"
     CDS             complement(22494..23432)
                     /gene="prmA"
                     /locus_tag="POB92_000848"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI6578367.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(23436..24575)
                     /gene="dnaJ"
                     /locus_tag="POB92_000849"
     CDS             complement(23436..24575)
                     /gene="dnaJ"
                     /locus_tag="POB92_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI6578368.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(24711..26543)
                     /gene="dnaK"
                     /locus_tag="POB92_000850"
     CDS             complement(24711..26543)
                     /gene="dnaK"
                     /locus_tag="POB92_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI6578369.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            complement(26612..27238)
                     /gene="grpE"
                     /locus_tag="POB92_000851"
     CDS             complement(26612..27238)
                     /gene="grpE"
                     /locus_tag="POB92_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI6578370.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            complement(27270..>27395)
                     /locus_tag="POB92_000852"
     CDS             complement(27270..>27395)
                     /locus_tag="POB92_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HDI6578371.1"
                     /translation="SDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
BASE COUNT         8358 a         5189 c         3730 g        10118 t
ORIGIN      
        1 ggagtatgaa atgaaccttt atcttctata atttaaaaaa tgagatttat gcatacatct
       61 gaccaaatgt gcataaatct catttcttta tattaatctt ggcaagactc acatacacca
      121 taaacttcaa gtttgtgttt gtgaatatta acaccaggta gtgataactt tatctgatct
      181 attggacaat aatctattac ctttgtatct ccacactttt cacagataaa atgatgatga
      241 tgatggtttg tacaagcgat tctaaacttc atttcaccat caagttctgt attttcaata
      301 atccctaaat ctttaaataa gtgcaggttt ctatatattg tgtcgaatga aattccagga
      361 taattttcat ccataacttg ctgtatatac tttgcgttta tatacttatc ttcttcgaca
      421 aaaatatcta acatatcttt acgtttatct gtatatttta aaccgttctc ttttaaaatt
      481 ttaatagcat catttgtatt cattgatatt agctcccttt ttaaacttca ttcgcatttt
      541 ctgataagcc attgtaatca taagtaaaat aacaagtaga actacaatta caccacccgg
      601 agaaatgtcc atatagaaag ctaggactaa gcctaatatt actgataatt cacctaaaaa
      661 tacacttagt aatatcaatt gcttaaaact ttttgttatt cgcatactta ttgcaattgg
      721 taacgtgatt aacgcactta ctaacagtat ccctacaaca cgcattgagg cagaaataac
      781 catcgctaca ataacaataa ataaaaattg aatccattta ggaatgccaa tgactttact
      841 atattcctca tcaaatgaca atataaataa ttctttataa aacaatgtaa taaacagaac
      901 aactatgatg gcaatgacaa taatcgttgt taaatcactt atattcactg cgcttattga
      961 gccaaatagc aatccaacaa tttcttgatt gaacccatca gctaatgaaa tgaagattgc
     1021 actcaaggcg ataccagcac tcattataat tggaatagca atttcttggt aagcagtgta
     1081 tgacgttctt aatttttcaa ttagaagcgc acctactatt gcgaataaga ttccaaacca
     1141 cattggatta ataaatacta gtgttggcat aatagtaagt aaaaacatac cgaaagatat
     1201 accacctaaa gttacatgac ttagagcatc agctataagt gatagtcgtc taacaacgat
     1261 aaaagcaccg attagaggcg caataaaacc tatcaagata ccactaatta aagagtacct
     1321 cataaaatca aaattcaata atgcatctat caattgtgac acgcctttcc attttaaata
     1381 aactcaaatc tttattaatt acaacattct cgattatgct gatgatcgac aaaacgtaca
     1441 ggatgtccat aaatttttga aatttcaact tcatcaagtg atttaaactc atcagttgta
     1501 ccatggaaat gcaaatgctt atttaaacat gctacttcag tagcagtatc tgctacaaca
     1561 ccgatatcat gagtaactaa gataatggtg ataccttctt gttttaattg atctaaagta
     1621 ttataaaatt cacttacatg ttttgcatca ataccattcg ttggttcatc aagtactaat
     1681 actgcaggtt ctgaaatcaa tgctcgagca atcattacac gttgttgttg accacctgat
     1741 aattctgcta tatttttatg aattaaatca cttatattca gtctttctag tactttaatc
     1801 actttttcat tatctttgct attaaatgtt tggaaaagac gttttgtctt tgttaatccg
     1861 cttaaaacaa cttctttaac acttgctggg aaacctgaat taaaggcatt tgctttttgt
     1921 gatacatagc ttaatttaat tgatgttttc ttatttttaa aatcaatacc ttcaacaaaa
     1981 atctcaccac tttgtaaagg taataaccct agaatcaact tcaataatgt tgatttacca
     2041 gcaccatttg gtccaacaat tgctaaaaat tcacctttat ttattttaat gtttatattt
     2101 tctaacactt ttttatgatc atagtagtaa ttgacatttt tcaattcaaa gactggtgtc
     2161 atcgtattct cacctcgcat tcaactatac aactcctagt aacatatgta aacagtaatg
     2221 tttacgactc aaaattagac aaaataaaga gatatgcccc cttcaagttt tatttatcgc
     2281 atttcttgaa gagagcatta tcattttatt gttgcataac cttatttttt aattctgggt
     2341 caaattgctg ttgttttaac atttcaattt caagtttata tggcggtttt ttatttttct
     2401 tatcttcacc aacataaggt gtttctaaga ttttcggaat atctttaaaa ctatcatgat
     2461 gcacaatgta atttaatgca tcaaaaccaa tgtaaccgaa gccaatattt tcatgtcggt
     2521 ctttttgagc gccacggtca tttttagaat cattgacatg aacaactttg attctgtcga
     2581 ctccaataat tttatcaaat tcatttaata cgccatcaaa gtcctctttc acattatatc
     2641 cagcatcatg cgtatgacat gtatcaaaac atactgataa acgttcgtta ttatgaactc
     2701 catcaataat acgtgctaac tcttcaaacg agcgaccaat ctctgtacct ttacctgcca
     2761 tcgtttcaag cgcaatacgt acattattgt cattcgttaa aacttcattt aatccttcaa
     2821 taatcttatt aattccggca tcaacaccag ctccaacatg cgcacctgga tgtaatacaa
     2881 tatctttagc gcctatagct tgcgttcttt caatttcttg ttgcaagaaa tctacaccaa
     2941 gattaaacgt ttctggtttg gttgtatttg caatattaat gatgtatggt gcatgaacaa
     3001 caatattaga taagccatat ttttccatca cttcatgacc tttagttata tttaaatctt
     3061 caatactttt acggcgcgtg ttttgaggtg caccagtata aatcataaat gttgtttcac
     3121 catattcata cgcttctata gcagaacctt ctaacatctt tttaccactc attgaaacat
     3181 gtgatcctaa taacataaaa cacacctaac cttttttgtt ttgcttacgt ttttgtctat
     3241 tttgctgctt actaaattgt ttacgctctt gacgtttcat tttttcaact tcttgtttaa
     3301 atttcttctt ataacctggt ttaactttgt ttttaatttt acttcgaact ttgttcttca
     3361 cttgattagt taaatggtca tctttgcgca ttcttgcttg acgctgattg tgcgctttaa
     3421 cttcttttaa ctcaccatct ttaatatcaa cagtattgaa tacaaaaccg cgatcttcta
     3481 ttaatgaaat attgtgttct tcatcaggac tataaagcgt aattgctaca cctttataat
     3541 tcccacgacc agttcgtcca actctatgcg taaagaagtc aatatcattt ggcacatcaa
     3601 aattgatgac atgactaaca ccttcaatat caataccacg agatgctaaa tcgctggcaa
     3661 taacgtattg gaattctaaa ttacgtatac gtttcatttg ttgtttacgt tcacgtggcg
     3721 ttaaaccacc atgaatcata ccaactttaa taccagcttc atttagtgaa cgtgctaaat
     3781 catttgcatt atctctacta ttacagaaaa taatacataa gtatggattt agtatatcaa
     3841 ttaaatttaa agttttttca actttagctg cacctttagt aggtattaaa aagaattcga
     3901 tgttcttttt attttgtttt ttactgtcga cagctacata ttctggatga cttaaatatt
     3961 tatttaaaaa tggttgtaac tgttgtggaa ttgtagcact aaacaccgca atatttgcat
     4021 tatcttccaa tcttgcagca atgtaatcta catcttcaat taatcctaag tcaatcataa
     4081 gatccgcttc atcaataact aaatatgatg ctaagtgcac atgtaaatgt cccgttttag
     4141 ctaagtcatt aattctagta ggggtgccta taatcaattg tggttgtgca ttacaacgtt
     4201 gtctatcttt ctctatatct gtaccaccaa taaaaacttt aactgaaaca ccagctttaa
     4261 attggcttaa atggttcgct gcatcgtata gttgttgtgc aagttctctt gttggtgcaa
     4321 ctacgattgc ttgtggttct tttatttcac tatcaattaa ctgcattaat ggtaataaaa
     4381 atgcatgaga tttccctgta cccgtttgag attgaccaat taaatttgtt ctctttagta
     4441 ttcttggaat aattcgattc tgaatttcag ttggtttttc aaaattaagg tctttcacag
     4501 cgtcaattaa actagattct agattaaatt gttcgaatgg atgttttgcc atgatttggc
     4561 ctccttatat gttcatcttt actattataa agtataaatg tgctatttta aataaaaata
     4621 ttaaaacctc gaaatttcaa aatttagaaa tcccgagatt ttagtttaaa tatattgaaa
     4681 tggatctgta ttaattgtag atgcttcaac atctatattt attttttctg tattgaaccg
     4741 attcattagt aacgttttta aaccttcttt catcacatat tcgctgtaat gattaatatc
     4801 aattaaattc acaccatgaa ttttagcatc taaggcatca tgatgtttaa tatcacctgt
     4861 aacaaagaca tctgcgcctt gttggacagc ttgatattca tatccaatac ctgaaccacc
     4921 aataattgca atacgtttaa ttttctgatt agactcacca acaaaacgga cacttgggat
     4981 atttaattta gatttaatat cagctgcgaa atcttccaat gtcatttgat tatccacttc
     5041 tgccataacg ccaagtccat aaagggatgt ttgttttatc tcaataaaat caaataccgg
     5101 tgtttcatat ggatggtatt gtttaattaa ttgctcagcc cttgactttt gatatacatc
     5161 tatcataaat tcaattttaa cttcatctac atattcaatt ttatcaattt gtcctattgt
     5221 tggattagct tcaccaactg gtttgaattg ccctcttcct tcactttcaa agaaacaata
     5281 ttcataatta ccttcttgcg ctaatccatt ttcactaagc ttatctttaa atggtccaac
     5341 attatcctta ggtatatatg tttgaacttt atagtataca tcttgttgat tatttattat
     5401 tgaaatgttc ttcaaaccca tcgccttcgc caacatcata ttgacaccat acggatttac
     5461 atctaaattt gtatgcatcg ctattaaatt aatgtcatgt tgaattagtt ttctaatgat
     5521 caaaccataa ccattagctt ttaatgatgt tacgccttta aagattagag gatgatgact
     5581 aataatagta ttataacctt tttcgattgc ttcatttact acttccaacg tacagtctaa
     5641 tgctgttaaa acaccagtaa cttcaacatc ttcatcacct attaacaatc ctacattatc
     5701 ccaagattca gcagtactaa atggcacatg atgatctaac aatgtcatta aatcagctat
     5761 tttcattact ataacaccct ttcaattaca gcaatttcgt cattaatttg agctaaacgt
     5821 tgatgatgtt gttcagtatt gagtttcgat ttaatatgat aaagtgcttc taactctctt
     5881 tgccattttt ttataaaata ttcatttttg ttgttcagca attttggtcc gaatttcaat
     5941 ccatcagatg atagctctat taattgtgta gaatattctg ctacaacaat ttcataaata
     6001 tggccttttt cttccattat tatttcatca attatttcat aattcaattg ttgtaatgtt
     6061 tgtcttaaat tttcagtttg gatattactt tgtaaaatca accttggatg ttgacttaac
     6121 ttatcttgcc catcttttaa aattttagca ataagtggtc cgcccatacc acatattgtg
     6181 atattatcga ttacgtcctc aggttgaata acacttaagc catcccctaa acgtacatca
     6241 attctatcta ctaattggtt tgcagctaca ttttttacag cagcttgaaa agggccttga
     6301 ataacttctc cagcaatacc gcattcgcat aaatggtttt gaattgcata gattggcaaa
     6361 taagcatgat ctgagccaat atccgcgatt gtaccttgtt ttaaaaatcg acttaccgtc
     6421 gttaatcggt tatttaacga aatcatttta ttctccttta tgaaaaaaga ggttgaacat
     6481 tattcaatgt accaacctca ctcaagttaa atcttaatct aaaaataaat aagcgcttat
     6541 tcatgtcttg gtatcaatca ttaaaattga tataaattaa tccataaagt ctttcaaacg
     6601 tttactacga cttggatgtc ttaattttct aagtgctttt gcttcaattt gtcgaatacg
     6661 ttcacgtgta acaccgaaaa ctttaccaac ttcttcaagt gttcttgttc tgccgtcatc
     6721 aagaccaaat cttaatcgta atacattttc ttctctatca gttaatgtat caagcacatc
     6781 ttctaattgc tcttttaata attcataagc agcatgatct gaaggacttt gtgcttcctg
     6841 atcctcaata aagtctccta aatgactatc atcttcttca ccaattggcg tttctaatga
     6901 aacaggttct tgcgcaattt ttaaaatttc acgaactttt tctgctggta aatccatttc
     6961 ttcaccaatt tcttctggtg ctggatctcg acctaagtcc tgtaataatt gacgttgaac
     7021 acgaattaat ttattaattg tttctaccat atgcacaggg atacgaatcg tacgtgcttg
     7081 gtcagcaatt gcacgagtga ttgcttgtct aatccaccat gttgcatatg ttgaaaactt
     7141 aaatcctttg ttaaagtcaa atttttcaac agctttaata agacccatat taccttcttg
     7201 gattaaatca aggaataaca taccacgacc tacgtatctt ttagcaatac ttacaactaa
     7261 acgtaagttc gcttctgcta gtcttgattt tgctacttca tcaccttgtt caatacgttt
     7321 ggctaattcg atttcttctt gtgcacttaa taagttaaca cgcccaattt ctttaaggta
     7381 catacgaact gggtcattta ttttaacacc tggaggggca ctaagatcac ttggattcag
     7441 tttctcgtca gtatctgaac tatctttttc attaactagt gaaatatcat tatcatttaa
     7501 ttgatcaaag aaatcatcca tttgatcaga gtcgatatca aaattctgaa gtttttcagc
     7561 aatttcttca tgacttaaat gaccctcttt tttacccttt tcgattaatt gcttcttaac
     7621 atcttctaat gttaatgtcg gatcaattgt ttgtttttta attttaactg tgttatcaga
     7681 catgaaacgg cctcccgatt ttaaatatga acattcgaaa tttattcaat attgctattt
     7741 taaactaaat tcttaattaa ttcatccata tttttaattt tattttacaa attggaacta
     7801 atcccaatat ttatttttca atagtgttgt ttaaatgcgg taactatcat ttacccttat
     7861 acattttgac ataatcatta ttcgtattat tctttaaaat cacatgctac atgcgttctt
     7921 tattcttagc aacaatttgc tgtaaatagt atttttgtaa ttctacatcg ccaatccttg
     7981 tagcttccct taatttatga ttcaatgact caattgtttc ttgtcctttt tcattaataa
     8041 cattgacata atcatcaatt tcattttcat atggttcgtc attcaaatta tattgttcta
     8101 agctaattag tgtttctctc aactcatttg aattaacata ctgcacagca tcactgatat
     8161 tatattgatc attttccgca taaaaatcat gtaagacttc gaatacatat ttaaaatgct
     8221 gatttgtgaa gttatcctta tcaacacttt cataataatt taaaaatgta tctttatctc
     8281 tcattaaatg ttttaaaaat gctcgctccg ctttttcttg acggctcaaa ttgtcaaatt
     8341 gtgccatacc aattggctca ggttcaatat aaccgccata ctcatcataa ccgccatact
     8401 catcatctgg ataataattg gctggtgctt gattgaattg tatttcgtta gctaattgct
     8461 caggactaac attgaaaaat ggtgcaacat catttaaagc cttttgttgc aaaatcgatg
     8521 atttcataag cgaaatatca tgacttagtt ctttcaaata acgttcatat gaaaggtcat
     8581 tatgtgcaat ttcatctttt aatatactca ctttataatg tgcaaatgac tttttgtcat
     8641 tttttacaaa agcagtaaat gcgtcgttgc catacttacc aatgtattca tccggatcca
     8701 tgcctgatgg caattgtata acaaatacat ttagcccttg ctgtaataaa tgttgacctg
     8761 ttttaagtgt tgcttcacta cccgcaaaat ccccatcaaa cattaatgtt atatttaatg
     8821 ttaactttcg tataaaagta atatgttcat ctgacaactg tgtccccatt gttgcaacaa
     8881 cgtttttcaa gccagcagta tcagatttta taacatccat aaaaccttct agtaatacga
     8941 tttcatctaa ttttctaatt gatttacgcg ctttatctaa gttgtataac aactttcttt
     9001 tttgaaagat aggtgtttca ggactattta ggtattttgg ttcttgaccg gtatatgttc
     9061 gacctgaata tccaacaatt cttccttgcg catttttcaa aggaaacata atacgatttc
     9121 gaaatctatc gtaataactg aaattttctt cgttacgtga taataatccg gcttcatatg
     9181 ctaattcaat atcgtaaccc tttttttgaa gaaaatcatg acaaaaatgt gagctatcgg
     9241 gtgcaaagcc aatgcctcgc tctttaataa gcgcatctgt aaaaccacgt tcttgtaagt
     9301 atgttaatgc ttgttcgcct tcgactgtct ttgttaaagc gtaataataa aattcttgta
     9361 ttaactcatg catttcaatc atttgtaaat catcagaagc aatttgaata tttgagttaa
     9421 attgtgttgc ctcaatatct acagcaacat taactctatc acctaattct ttaaccgctt
     9481 caacaaatga tatgtcttta atttcttgag taaattgaaa aacattgcca ccttttttac
     9541 aaccaaaaca atgacagatt tgtttatctt cagaaactgt aaatgaaggt gtcttttcat
     9601 catgaaaagg acacaaacct atataattgc gtcctctctt ttctagtttt acatattcac
     9661 ttaccaagtc taaaatgtcg gttttatctt ttatttcatt aatgatcgat tgatctattc
     9721 gcaaattaaa tcacctatta agcattagtt atactattat acctaatttt tgacaaaaat
     9781 gaaatcattt cgatttattt tgttcaatat aatggattat atcgtttgct gtttcttcga
     9841 ttgctttttg agaaacatca atgacaggac atccaatttc acttacgatt tcttcaaagt
     9901 aattcaattc ttcttggatt cgagcttctg ttgcataacg agctgtgtca cctagtccca
     9961 attgttttaa tcgctctttt ctaatgcgat ttaatttttc ttcacttatt ttaagtgcga
    10021 tacatttctt tggatcaata tcatataagc catctggcgg tgtcacttct ggtacaatcg
    10081 gtacattcat aactttgtaa cttttatgcg ctaaatactg ggataatggt gtctttgaag
    10141 ttctcgaaat accaagtaaa acaatatcag ctttaggtaa tcctttagga tctttaccat
    10201 catcgtattt aactgcaaac tctatcgcat caattttctt gaaatatgca tcatctaatc
    10261 tatgaacgat acctggctca ttataaggtt tttcttcaac cgaagctgat aataaatcca
    10321 ttaatggccc catgatatcg acagacttca attggaattc tgctactttc tcactcatat
    10381 attgcttcat ttcaggttta ataagtgtat aaacaatgat agcatttgta tcttttgcaa
    10441 cttgaatcac ttcatcaaca tcttcaaaag attcaatata tggatatctt aataattcat
    10501 ttttacattg cttaggattg aattgtgaaa tacctgccct agcaactaac tctgccgttt
    10561 cacctataga atctgaagct acgataattt taattttttc cattacttct cacctattct
    10621 ttatataatg ctactaataa cttagctatt gttgttttgg aaattcttcc aattacttca
    10681 tacttttgat tatctttttt tcttacaatt ggaatcgaat caatttcttt ttcaatcatt
    10741 ctatctgctg cgtatatgac taattcgctt tcctctaaat aagtgacatt aggcatacgt
    10801 gtcatattta cactgatagg tactgtatga atatctgctc caatcattga agctcttaat
    10861 aaatcttttc ttgaacacac accaacaaaa tcgttatctt cattaataat aaataatgta
    10921 cttacatctt ctaaaaaaat tgtacaaata gcgtcataaa ctgttgtatt ctctcttagc
    10981 acaacaggtt gagacatata gtccttaacc tcaaattgtc gaagtttttc attaaaaaac
    11041 ttaccttttg atttacctga ataataatat ccaactcggg gacgcgcttc taaaaaacct
    11101 gacattgtta atatcgctaa atctggtcta agcgttgctc ttgttaaatt caacttatct
    11161 gctatttgtt caccagtaat gggtccttta gttttaacaa tttcgatgat tcgttcttgt
    11221 ctttgactga gttctatagg tcttcacccc ttttttattc acttattatt ataccgatat
    11281 ttctttcatg tcacaaacac attaatttta cttgccttta aataatctat caattagaat
    11341 aaacgtacag cgagttaagg atagtgtaag cttaacaata agattggcgc aacgaatcat
    11401 tttaaaataa aagcgagtga ctacactaat ttgggtggaa ccgcgggtta aatacgtaac
    11461 ttattttgta ttatatacaa tttaaaatat taactcgtcc catggaagta aagcatattt
    11521 actatgctgt tattttccat ggggcgtttt catgtatgag gagaggtaat tatggcaaaa
    11581 gatatggata caattgtttc attagcaaaa cacagaggtt ttgtgttccc tggtagtgat
    11641 atttacggtg gtttatcaaa cacatgggat tatggtcctt taggtgttga attaaagaat
    11701 aatgttaaaa aagcttggtg gcaaaaattc attacacaat caccgtttaa cgttggtatc
    11761 gatgctgcaa tcttaatgaa tccaaaagta tgggaagctt caggacactt aaacaacttc
    11821 aacgacccaa tgattgataa taaagatagt aaaattcgat atcgcgctga taaattaatt
    11881 gaagattata tgcaagatgt taaaggtgat gaaaacttca ttgccgatgg tttaagtttt
    11941 gaacaaatga aaaaaattat tgacgatgaa ggtattgttt gtcctgtaag taaaactgct
    12001 aactggactg aaattcgcca attcaattta atgtttaaaa cattccaagg tgtaactgaa
    12061 gattctacaa atgaaatttt cttacgtcct gaaacagcac aaggtatttt tgtaaactat
    12121 aaaaacgtgc aacgttcaat gcgtaaaaaa ttaccatttg gtatcggtca aattggtaaa
    12181 tcattccgta atgaaatcac tccaggtaac ttcattttca gaacaagaga atttgaacaa
    12241 atggaacttg aattcttctg taaacctgga gaagaaatcg aatggcaaaa ttattggaaa
    12301 acttttgcaa gtgactggtt aacaagctta aatatgagca gtgaaaatat gcgtttacgt
    12361 gatcatgatg aagatgaatt atctcattac tcaaatgcaa caactgatat tgaatataaa
    12421 ttcccatttg gttggggtga gttatggggt atcgcaagtc gtacagactt cgacttacgt
    12481 aaacatgctg aacactctgg tgaagatttc agataccatg atccagaaac gaacgaaaaa
    12541 tatattccat attgtatcga gccatcactt ggtgcagatc gtgtaacatt agctttctta
    12601 tgtgatgcat atgatgaaga aggcgttgaa ggtagtaaag atgcacgtac agttttacac
    12661 ttccatcctg cattagcacc atataaagca gcgattttac ctttaagtaa gaaattatct
    12721 ggcgaagcga ttaagatttt tgagcaatta agttctaaat tctcaatcga tttcgatgaa
    12781 tcacaatcta tcggtaaaag ataccgtcgt caagatgaaa tcggtacacc ttattgtgta
    12841 acattcgact ttgattcatt agaagataat caagttacag tacgtgacag agattcaatg
    12901 gaacaagttc gtatgccaat ctcagagtta gaagctttct taactgaaaa aacaaaattc
    12961 taattaaaaa gactctccac catatgtggt ggctatataa tttataaaaa gagatactgg
    13021 ttagtaaaat tgccagtatc tcttttctat tcgaaaaagg tattcaaata aacacatcta
    13081 tgtggctact tgaatacctt ttattatttt atgttattgt tccaatcttt ttaattggtt
    13141 gattagtttc tgacttttaa aaaacatacc tgcatattca cgatataaca ttaaaatgat
    13201 atctgacatt tcatcaataa tttcttgatg gatattcaat gaattcattt tatctatcgg
    13261 taatttttgt aatacatcta ataaatatag tgttttattc gataatataa ctgcatgtac
    13321 atctttagaa gcctcttgcc ttgaaatcgc accgtcaaac ttaaaactat aacctattaa
    13381 atctgcttgt gtgtcattac cactcacagc acagcgatta aatgatgcag taaaaccaaa
    13441 tcgtttcata cactttaaca taactacgac tgacattaac tgtgcagatg tacctgattc
    13501 tattttttca agaacaaatt gtaataattg atagttatat ggtgcaatgt caccttcatc
    13561 cattgagcgc tcaatagttt cagctgccag agacgcataa ctgcttacga aaaggtccat
    13621 ttgtaattta taatgttgac taataacatc tacagaattt aacgttccca tacctcgcca
    13681 ctgattgtaa ataaacaaac cataaacaaa caattgcgtt tgcgcttgta aacccgtctt
    13741 aactttttta gcacgcctag ccataagtgg tacttttgca ccatgctcat ttaaaatcgt
    13801 gataatttta tcagattcac cataatcaac tgctttgatg ataatccctt tttggcgcat
    13861 taacaattat cttcaccact tttaagatta atcttggtct tcaacataac caatttggcg
    13921 aataaagtta actttgtttc gccagtctct ttgaactttg acccataatt ctaagtaaac
    13981 tttagagcct agaagcattt ctatatcacg tctcgcacgt ttccctactt cttttaactt
    14041 tttaccgcct tttccaatga caattccttt ttgcgaatct ctttcaacat atatagttgc
    14101 ttcgatatga acacgatctt cgctttcttt aaccatacgg tccacattaa caccaatcgc
    14161 atgagggatt tcttcacttg taagatgaag gattttttca cgaatgattt cacccactac
    14221 aaattgttca ggatggtctg aaatttgatc atctggataa tatttaggtc cttcgggtaa
    14281 atacgtcttt aaaacatcaa taaaatgatc tacatttagc ccttctaatg ctgaaatagg
    14341 tacaatctct gtaaagtcca tataactttg atattcttca atctttggca ttaattcatc
    14401 tggatgcact aaatctattt tatttaatac taaaaatact ggtgtcttaa catttttcaa
    14461 catttctata atatattcat cacctcgtcc aatttcctca ttggcattaa ccataaacat
    14521 gattgcatct atctcagata atgtattttt agcgactttc atcatatagt cacctaattt
    14581 gtgtttaggt ttatgaatac ctggcgtatc aatgaatata atttgcgcgt catctcttgt
    14641 cataacacct tgaattttat ttctagttgt ttgagcttta tcggacatga ttgctatttt
    14701 atggccgatc actctattaa caaatgttga cttccctaca tttggtctac ctataattga
    14761 aacaaatcct gatttatgtt ctgtcattta ttctaaatcc tttcctgaaa atccaaatgg
    14821 tagtaactct gcgactgtca tcataaccat atctccttta tgatttgtca tatacacagg
    14881 catatcatca tcacataatt cctttaaaac ttgacgacat gcaccacaag gtgatgacgg
    14941 tttatctgca tctacggtta cagttattga ttcaaaatca cctggtctgt atccttgaga
    15001 aattgccgat accaaactag ctcgttcggc acatatcgat aatggataag aagcattttc
    15061 tacattggta ccataaaaag ttctaccgtc tttcgttttt aaataagctc ctactttaaa
    15121 ttgactgtat ggcgaatatg attcttgttg tgcttttcta acttcttgaa aataatgagg
    15181 ttgataactc atatatgcct ccctaaaata acgctataaa atgtggtaaa aatactatta
    15241 aacctataat aaatgctaat attgaaacta taagtacact aaaagccgct atatcttttg
    15301 cgtatttagc taaatcatga tattcaacgg tcactaaatc gacaacatat tcaatagcag
    15361 tgtttaaagc ttcaacagtg agaactaatg caatagcaat gagtataaat atccactcaa
    15421 tacgattaat atttagtacg agaccaaaga caatagcaac aatcattgca aacacatgta
    15481 aaagaaattt atagtctttt tgaattaaga ttttcagccc atcaagtgca tatttaaacc
    15541 ttttcataat tagcctcgtg ttaatccata tgcgtttaat attgtatctt gtcgaccaaa
    15601 catttccttt tcatccgctt cagtcatatg atcataacct aatagatgca aaaatccatg
    15661 taatgctaaa aatcctaatt ctcgttcaaa agaatgtccg taattgtttg cttgttcttg
    15721 cgctacatcc gtacagataa ttatatcccc taaaacacgt ggtatatcaa gaccactaaa
    15781 atcaatctct ggctcatctt cttctaaagc aaatgagatt acatctgtaa ctttatcttt
    15841 atctctatat gttcgattaa tttcttgtat ttcttgttta tctacaaatg taacagaaag
    15901 ctcagcatcg tcttctatat gctcttcttt tttagcaaat tctaataaat cttcaatttg
    15961 tttataccac gcatctttaa ctaagcctgt gtgatcgcta aaatctatcg taaacattta
    16021 attctctcct tcataatgtt caatgatctt acttaccaat ggatgtctta ctacatcgct
    16081 ctgatctaat ttcaatatac ttataccttt aacgttgtgt aacctactga ccgcttcctt
    16141 aagtccactt ttaacacctt taggtaaatc gatttgagtt tggtcaccag taactaccat
    16201 ttttgagcca aaacctagtc ttgttaaaaa cattttcatt tgcgcatgtg tcatattctg
    16261 cgcctcatca agaattacaa atgcatcttc taatgttcgt ccgcgcatat atgcaagtgg
    16321 cgctatttcg ataatgcctc tttcaataaa tcgctctgtt tgttcacgcc caagaacagt
    16381 atatagacca tcatataaag gtcttaaata tggatctacc ttttctttca aatctcctgg
    16441 taaaaatcca agtgactctc ctgcttcaac agcaggtctt gttaatacaa tacgtttaac
    16501 agcaccttta cggagttgct ttgctgcata aactacagct aagaatgtct tacctgtacc
    16561 agcaggacct ataccaaata ctaaatcatt atttttcatg gcattaacat atatacgttg
    16621 ccccatcgtt ttcgcacgaa tcgtcttacc aaatgcatct ttagttatct cttcatcata
    16681 taaatctaac agatgttgaa ttgtgttatt atgcgccatt ttaatagctg cttcaacatc
    16741 tttaattgta atattattac ctaaatcaat aaccttcagc aaattgatta atactgattc
    16801 cgctttttct acgttttcta tttttgtacc tttaacggca acttcttgtc ctcttgcatg
    16861 gatgacaaca tcgaaactct cttcaattgc ttttaaatgt tcatcattat ttccaattaa
    16921 agcttgagat tgattcatat cgtctatttg tataattcca ggcatacacg cgctcctttt
    16981 catatatcat ttatattcat tctatgatgc tttaatatct acttaattat atcatgcaat
    17041 tgattgattt aaacatcttt gcattgactt actcattttt ctttaattta aaataatcat
    17101 ttatactttt caccttgtca taaaagcaga ttcaatttta gactaatcaa ttttttgttg
    17161 taatttaaat aactaatatt tctcgaaaga ttctctttgt ttttcaattt ttcttaacgt
    17221 aaaaaaacaa gcaactacca ttacgatagt gcttgttttc aaaaaatttt ataattgttt
    17281 tggtttagct aaaatttctg accatatcat accattgatt acttcatctt tatcaaattg
    17341 gaacgctgat ttagtcaaat ctttttcgac attttgagaa ttaagcaact gctttaattt
    17401 caaacgtttt gtacgttcag ataagtattt atcctcaata atatctctag cacgtttttc
    17461 cattttagca atttgctttt ctttttctct atcaatgtca gtacgaatcg ttttaatatc
    17521 atctctaagt gatttttcta attgacgtct aatttcgtca gaattatctt gtctagaagg
    17581 tctatgtttc tgctccaccg gttctgcttt aggcactgta attggtttct ctgtcatcgg
    17641 ttttgtttct ggttgttttt taggtgccat aggttctaca acaggtttcg atttaggctc
    17701 ttcctttgga agttcatcga ataaaggtgg taacgtatca tcatattttc gtttcgatga
    17761 tttcttttct tcttcattta attcttcact tatttcttta aacgttcgct caatttcttc
    17821 aaaaaagcca cctttttttg gttcattatc ggtagatgtt ttttgaggtg gcttttgatt
    17881 ttgtctatct ttatgactat tttcgcgcat agtagtaatg atagaaatga tcactgatat
    17941 gacaaaaatt agaataccga cactcattta atcacctctc gacttaatgt tcaggtgact
    18001 catcatcact ttgatcagtt cgtttattaa ttgcatttct catgcctgta tcagcttcga
    18061 tatttttcaa attataataa tctttaacac tgatattacc tgaacgtaat gcttcagcca
    18121 tagctaatgg tacttcagat tcggcttcaa ctactttagc atgcatttct tgtacacgcg
    18181 ctttcatttc ttgctcagtt gctacagcca tagctctacg ttcttcagct tttgcttgtg
    18241 caatattttt gtctgctaat gcttgttcag tttgtaagtc tgcaccaata tttttactaa
    18301 tatcaacgtc agcaatatca attgataaaa tttcaaatgc agtacctgaa tctaaacctt
    18361 tgcttaaaac tgttttagaa atattatctg ggttttcaag tacttctgta tgatgcttac
    18421 tagaaccaat tgttgaaacg ataccttcac caacacgtgc gatgattgtt tcttcaccag
    18481 caccaccaac aagtcgagca atattagctc taactgtgat acgagctttg gctttcactt
    18541 caataccgtt cattgctaca cctgcgataa atggtgtttc aatgacttta ggattaacag
    18601 acatttgaac cgcttctaat acgtcacgtc ctgcaagatc aattgcagca gcacgttcga
    18661 aaggaagatc aatgtcagca cgttgtgcag caatattagc gtcaacaact ctgtcaacat
    18721 ttcctcctgc tagataatgc gattctaatt ggtttgttgt taatgctagt cctgctttat
    18781 gcgctttaat taatggcgct ataacttttc ttggagatac acgacgtaaa cgcataccaa
    18841 ccaatgtacc tataccaaca tgaacgccag ctgctaacgc tgaaatccat aaaccaatgg
    18901 gtacaaatga gaataaaata agtaatgcaa ctactataat aactgctatt acgataaaac
    18961 ttaaactaaa catggtatcg ctccttttaa ttaatctact tccctcacaa ctactcttgt
    19021 tccttcaact tcaaggattt ttaccgtttt attgcgtaaa ataaagttgc catctgaaac
    19081 agcatcaata cgttcatttt cacaaaaaat aatccctgca ggtcgaagat ctgtaactgt
    19141 ttgagcagtc tttcctacga ggtgcgagcg gttatcatga gaattgtaac ctgactcaga
    19201 attagttgaa tcttttaaga taactttatc caaaaacgga atctttctgt tgaaaatctt
    19261 cactaatatc acccattcta caatcgttaa aatcaaggca acgataacat tcgcaagcat
    19321 aaatagcaaa ttatcaccga gcgttgttat gcttatagtt atcagtatca tgccaataat
    19381 accaattact gcaccaacta caaataattc aattacaact aatataacgc caattgagaa
    19441 tattaagata gaatgcatat tgacatttcc ttggattaga aatcccaaaa ataaaataag
    19501 taatgataat gtggcgataa taccagctgc attgattttt ttagagtaaa gttgatatac
    19561 aaatcctaag aatgtcaaac aggttaatat taacgtaaaa ataggttgaa caattatatt
    19621 actaacttgt tcaacccaag tatctccagc cgttgattcc aagatagttg tcatttgtaa
    19681 aaaattatta taactcacaa tttcacctcg ccctcacatt aattatacat gaaattgaat
    19741 atactttata gttataagaa cctatagaat atcaatttga ttatttataa actatataac
    19801 tttttttaat taaaaattga caacaaaaaa acatcacata ttagtgtaag acactaactt
    19861 gtgatgtttg ttttatataa ttaatattcg tgttgaggga gtcaacagag gtattaatta
    19921 tttgaattta cgtttacgtg cagcttctga tttcttttta cgttttacgc ttggtttttc
    19981 gtaaaattca cgtttacgta cttcttggat tgttccactt ttagaaactg aacgtttaaa
    20041 tctacgtaac gcatcttcaa gtgattcatt tttacgtact actgttttag acatctgtat
    20101 ttccctccct ccaaatatca acggaacttt atagtcaatt gcacagtaaa actatgcatt
    20161 tataagtata atatatctgt aatttatggt caattagtaa attgtttttt atttgaaaca
    20221 tatttcacat caaaatcaca aagactttta gattttgttc taaaaatctc ttaataattt
    20281 atttaatgag aagagttgct tatatagtaa attgtgaagc cgttaaaaca acgttacaaa
    20341 acctatatct ttaatacgga accttatggt atgaatcaag gaatacttaa actaaaactt
    20401 ctctatcaga tttatttgtt gcgaaatcaa caactttaat tgcttgccct tcatttaatg
    20461 gataatttgc ttgcgtaatt ttaactttta caatttgacc tatgagtgat tcgtcacctt
    20521 caaattgtac tttcatataa ttatctgcat atccaactaa tgtgccttct gtgtcaccct
    20581 gttcctcagg aattacttca agcacatctt gatcaaattt agacgcatat aactttccga
    20641 gttgattgct tagcgtaatt aacttatgca cccgttcatt tttaatttct tcatcaattt
    20701 ggtcatccat tcttgcagct ggcgtgccaa ttctaggaga ataagggaaa acatgcagtt
    20761 cagagaactt atgctttacg ataaaatcat atgtttcttg gaactctgct tcagtttcac
    20821 ctgggaaacc aacaattaca tcactcgtaa ctgccaagtc tggtaaagct ttatgcaatt
    20881 ttgttaatcg ttctgaaaat ctatccattg tatacttacg tctcatacgt tttaatactg
    20941 tatctgaacc agattgtaat ggaatatgca aatgacgcac aacttttgtt gaacgttcta
    21001 aaacgtcaat tacttcatct gtaagttgac ttgcttcaat tgaagaaatt cgaattcgtt
    21061 ctaatccatt aatcgtttca agatcacgta ataattgggc caagttataa tcttttaaat
    21121 cttgaccata tccacctgta tgaattcccg tcaatacaat ttccttatat cctgaattca
    21181 ctagttgcgt cgcttgttca actacttttt ccggatctct tgaacgcatt aagccacgag
    21241 cccatggaat aatacagaat gtgcagaagt tgttacaacc ttcttgaatt tttaatgacg
    21301 cacgtgttct atctgtaaaa tatgggacat ctaattcttc atatttacga tttttcatga
    21361 tatttccaac accattaatt ggttggcgtt ctttacggaa ttcatcaatg taacctaata
    21421 gtttatgtct atcttgtgta ccaactacta catcgacacc aggaatttcc ataatttcag
    21481 ctgatgaagt ttgcgcataa caacctgtta cacagattac agcatcagga ttttgtctta
    21541 ttgcacgtct aattatttga cgactttttt tatcacctgt attcgttact gtacaagtat
    21601 taataacaaa tacatcagca ttcgcttcaa agtcaacgcg ctcatagttt gcttctttaa
    21661 ataattgcca gattgcttca gtttcataat ggtttacttt acaacctaat gtgtgaaacg
    21721 caactgttga cataaatatt caccccatta attctttttc ataacttatt gcacttaacg
    21781 catacaatgg cgcagtttct gcccgtaaaa ttctcggtcc aagaccaaca actgtactag
    21841 tattactaaa taatgaaatt tcattttctg acaaaccacc ctcaggacca aaaatcatca
    21901 acactttatc ctgagccttg aattgttgta aagtttgctt gaaattgctt aactcaccat
    21961 cttttgcttc ctcttcatat gcaataagaa tatagtcata attatcaata gtatcacaaa
    22021 ttaattttaa attcgactcg aattgaatag atggaatcac taaacgatag ctttgttcag
    22081 cagcttcttt aattattttt tgccaacgct ctatcttttt ggcaactttt gcctcgttta
    22141 atttaacaat tgaacgttcc atgctcacag ctataaatga tgaagcaccc aattcagtag
    22201 ctttttgtag caaccactca tatttgtcag ctttgattag tccactgcaa atcgtaacat
    22261 caactggcaa ttctgtatta atattttgtt tttcttttaa atcaactgca attttatcac
    22321 ttgttatatc agcaatttca cataaataaa ctgtttgatc attaaaagtt aaaataattt
    22381 tactaccaac atcatatctc attacatttg ttatatgatg aatatcttct ttttttgtaa
    22441 taaaaaaacg ctgacttaca tcagcgtttt ggtctatgaa ataacgttgc acattattca
    22501 ctcactttct ggccaacaag acaaacccaa ccgttgtcat gttgttctga aataatttta
    22561 aaacctacac gctccatatg tgactgtata ccttcatact tctcttttat aataccagaa
    22621 gtaataaaat aaccgccttc atttagagta ttataagcat cttcaatcat ttcatcaata
    22681 atatgcgcta aaatatttgc tattacaata tcaaattttt ctgtttcgtc tttcaataag
    22741 ttacctggaa cagcttcaat taacgtttca caatgatttc ttctgaagtt ttctttagct
    22801 acactcactg ccatttcatc aatatccaac gctttaatac gttttacacc gattagatga
    22861 cttgcaatac ttaatatacc tgagccagta ccaacatcaa ttactgaatg ctgtggcaat
    22921 acatatgttt ctattgcctt caaacacata cttgtagtcg gatgatcacc tgttccaaaa
    22981 gccatacctg ggtcgagctc aatgcaaagc tcttcatccg cttctttagc atatgtttcc
    23041 caactaggaa ctattgtgaa cttcttcgac gctcggaatg gatggaaata gtttttccat
    23101 tcattttccc aatccgtctc tgcaataatt tgctcactga attgaacgtt atgttgatca
    23161 agttcatcta aatttaataa ctcatcttta atttgctgtc gcaacttatc atcataagtc
    23221 atttcattaa aataggcttt caatcttact cccttatctg gataatcctc ttttttcaaa
    23281 gcgtaaattt caccgtattt atcttctggt tggttaatta aatcatctga atcttctatc
    23341 acgacaccat ttgatccatg attttcaagt atattggtag ccaattctac tgcttcatga
    23401 ttaataataa ttgaaagctc tgtccagttc atactttatt ctcccttaaa gaatcttttt
    23461 gctctatctt taaaattcga aggttgttca ttaatttctt caccatttaa ttgggcaaat
    23521 tctttcatta gttctttttg tctatctgtt aatttagtag gcgttactac tttaatatca
    23581 acatataaat ctccgtatcc atagccatga acatttttta tacccttttc ttttaagcgg
    23641 aattgcttac ctgtttgtgt accagcaggg attgttaaca taacttcatt atttaatgtt
    23701 ggtattttta tttcatcgcc taaagctgct tgtgggaagc taacatttaa tttgtaataa
    23761 atatcatcac catcacgttt aaatgtttca gatggtttaa ctctaaatac tacgtataaa
    23821 tcaccagcag gtcctccatt cacgcctgga gagccttcac cagctaatct aatttgttgt
    23881 tcattgtcga caccttcagg tactttcact tctaatttaa ctgttttatt ttcagtacct
    23941 tttccgtgac atgttggaca agcttcttca aattcttgac cacttccatt acatttagga
    24001 caaacttgtt cagtacgaac tctacctaaa attgtgtttt gttctacagc tacatgacca
    24061 gcgccattac agtaactaca agtcttttta cttgttccag gctttgcacc atcaccatga
    24121 catgtttcgc atgttacatc tttacggatt gaaatttctt ttgttgtacc aaataccgct
    24181 tcttcaaatg ttaatgtcat tgtatactga agatcatcac ctttttgcgg tgcatttgga
    24241 tctctttgtc tgccgccacc gaagaaagag ctaaagatat cttcaaaacc gccgccaccg
    24301 aagccactaa aaccgccaaa gtcagagcca ttgaatcctt gtccaccaaa accttgtgga
    24361 ccatcatgtc caaattgatc atagcttgcg cgtttattat catcacttaa aacttcatag
    24421 gcttcagaaa tttctttaaa cttttcatct gcaccttctt ctttgttaat atctggatga
    24481 tattttttcg aaagctttcg atacgctttt ttgatttcat cttttgaagc atctttacta
    24541 atgcctaaaa cttcataata gtctcttttg gccacagcta tctctccttt tcttaattaa
    24601 ctcatatagt ttaacgtaat atgtcatact atccaaataa aaagccaaag ccaatgttct
    24661 attgactttg acttttcaga tcatgacaac attctaattg tattgtttaa ttattttttg
    24721 tcgtcgtctt ttacttcttt aaattcagca tcttctacag tactatcgtt gttttgacca
    24781 gcatttgcac cttgtgcttg ttgttgctgt tgagccgctt gctcatatac ttttgctgat
    24841 aattcttgaa tcactttttc aagttcttct tttttagatt taatatcttc tatatcttga
    24901 ccttctaaag cagttttaag agcgtctttt ttctcttcag cagatttttt atcttcttca
    24961 ccgatatttt cgcctaaatc agttaaagtt ttttcaactt ggaatactag actgtcagct
    25021 tcgtttctta agtctacttc ttcacgacgt tttttatctg cttcagcgtt aacttcagca
    25081 tcttttacca tacggtcgat ttcttcgtct gataatgaag aacttgattg aattgtaatt
    25141 ctttgttctt tatttgtacc taagtctttt gcagttacat ttacaatacc gtttttatcg
    25201 atatcaaacg ttacttcaat ttgaggttta ccacgttcag ctggtggaat atcagtcaat
    25261 tggaatctac caagtgtttt attatccgca gccattggac gttcaccttg taatacgtgt
    25321 acatctactg atggttgatt atctactgct gttgaataga tttgagattt agatgtagga
    25381 atcgtagtgt tacgttcaat taacgtattc atacgtccac ctaaaatttc aatacctaaa
    25441 gatagtggtg ttacgtctaa taatactacg tctttaacgt cacctgtgat aacgccacct
    25501 tggattgcag ctcccattgc cactacttcg tccgggttta ctcctttgtt aggttcttta
    25561 ccgatttctt ttttgacagc ttcttgtact gctggaatac gagttgatcc accaactaag
    25621 ataacttcat cgatatctga gtttgttaag ccagcgtctt tcattgcttg gcgtgtaggt
    25681 tccattgttc ttctaattaa tgaatctgat aattcttcaa atttagaacg agttaagttt
    25741 acttctaagt gtaatggacc gttttcacca gctgagataa atggtaatga gatttgagtt
    25801 tgtgatacac ctgataagtc ttttttagct ttttcagcag catctttcaa acgttgtaat
    25861 gccattttat cttgagataa gtctacgcca ttttcttttt tgaattctgc aactaggtag
    25921 tcaataatta cttggtcaaa atcatcaccg ccaagtttgt tgtcaccggc tgttgatagt
    25981 acttcgaata caccgtcacc taattctagg atagatacgt caaatgtacc gccacctaag
    26041 tcaaaaacaa gaactttttc atctttatca gttttatcta aaccatatgc taatgctgca
    26101 gctgttggtt cattaatgat acgctcaact tctaaaccag caattttacc agcatcttta
    26161 gttgcttgac gttcagcatc gttaaagtat gcaggtactg taattacagc tttgtcaact
    26221 ttctcaccta aatagctttc agctgtattt tttaagtttt gtaaaatcat agctgagatt
    26281 tcttgtggtg tgtatgattt accttcaata tctactttat aatcagtacc catatgacgt
    26341 ttaatagatt gaacagtgtt tgggtttgta atagcttgac gtttagctac ttcaccaact
    26401 tgagtttctc catttttgaa agctacaaca gatggtgttg tacgtgaacc ttcagggttt
    26461 tgaattactt ttggctcatc gccttctaat actgttacac atgaatttgt tgtacctaag
    26521 tctataccaa taattttact cataataaaa ttcctccatt taatcattaa attaatttaa
    26581 ttttaaacaa tgtcttttcg ccaaatttaa gttattggtt tactttgacc attgatggtc
    26641 ttaatactct atctttaagc ttgtatcctt tttgtagttc ttgagtgatt tcgccagatt
    26701 caaaatcagg gttatcatct tgaactacag cttggtgaat atttggatca aatgcttcac
    26761 cttcagtttt aataacttca agaccattat cttttagtgc gttaatcaaa ctttcatgca
    26821 ccatttgtac acctttttga agagatttaa aagtctcatc atcaccttca atttgaagtg
    26881 cacgttctat attgtctatt gctggtaaaa tatctgttaa cacacgttgt gcttgatatg
    26941 ttttatttat ttcattttct ttttgaattc tacgcttata attttcaaac tcagcgtaga
    27001 gccttaaata tttctcttcg ttttcatctg ctaattgttg aagttcatta attttttgat
    27061 cttttggatc tatttcttca ataacattct cgtcagacgt ttcttctatt gcttcatctt
    27121 gtaaatgacc tttactttct tcagcttgtt caactgaatc atcaatattt tgtttgacgt
    27181 ttgtttcttc aactgttgat tcagtgtttt tttcaactga ttcgtcttta tttgtcattt
    27241 tctgtcctcc aatactttct aatccatcat taccaaattc tatttaataa ttgaatgaca
    27301 ttttgataat gcatagctgt aggtccaatc acagcgattt gaccttttaa cgtttcatca
    27361 aaatgatatt gacttgttac aattgaaata tcact
//
LOCUS       DAMZLE010000022        26120 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMZLE010000022 DAMZLE010000000
VERSION     DAMZLE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.022"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            27..1814
                     /locus_tag="POB92_000853"
     CDS             27..1814
                     /locus_tag="POB92_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDI6578372.1"
                     /translation="MSEFKRHQQTSINVQKQANLIWNVADILRGLYKPHEYGKVILPM
                     TVIKRLHDTLLKTRDKVLKTAENTQSINDVMRERLLKNASGYSFYNTSLYTFETLLAD
                     PANIESNFRAYLNGFSENMQDILNNFKFDVEITTMADNDVLFYVIQEFNKADAYLGPD
                     KMTSTDMGYVFEELVRKFSESYNEEAGAHFTSRDIIYLMTDLLLIEDKDTLFKEHVFK
                     TVYDQTMGTSQMLSAMTERIHDMNDTAEVATFGQELNPETYAISKADTMIRGGDPENM
                     ALGSTLTSDRFEGFTFDYCISNPPFGVDWKKDQKAVKAEHELGELGRFGVGLPRVSDG
                     QLLFQLNGISKLKETGRMAIIHNGSALFSGNPGAGESLIRQYVIENDWLEGIIQLPND
                     LFYNTGISTYIWIITKDKSPERQGKVQLVDASNMYEKRRKNIGEKRVDISEACREMIV
                     QAYGEFNDKEYYLGDGTVESKILKNESFGFTRVTIERPQRDENGDIVYKKNGSKSVDT
                     NLRDTEDIPLTEDINEYFEREILPFNQDAWMDRKKDKIGYEIPFTRLFYKYTPPEPSE
                     VISERIKQLEESIIKNFQALSGKEVDTNE"
     gene            1807..3117
                     /locus_tag="POB92_000854"
     CDS             1807..3117
                     /locus_tag="POB92_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6578373.1"
                     /translation="MSREMKYSGIEWIGYIPKYWTITKLKNIIDFISGYAFKSELFTI
                     SDNNKKVITIKSFNTKEIILDNLSYSNESLKFPTKYLLKNNDILFAMSGGTTGKNLLI
                     EQVDDLYYINQRVGIIRSSFSKFIYYYINTGLFSEYINLFSSGSAQPNISATDIQNFI
                     IALPEKETIKKIEIYINYQLKIISNIIDTTYQSIEELKKYKQSLITEAVTKGIDPNVE
                     MKESGNDWIGSIPSNWSVRKIKHDFNLKGRIGWQGLTSNEYQTVGPYLITGTDFKKGI
                     IRWDSCVRISEERFEEAPDIHIKENDLLITKDGTIGKVALATNVPKKVSLNSGVLLIR
                     EKLKNTINKKFMYYNLLSNMFWNWYNSNNQGASTIKHLYQGQFYNYSYAIPLLHEQQQ
                     IVQYLDDKVSTIDRLIEDKTKVIKELENYKKSLIYEYVTGKKEV"
     gene            3130..6159
                     /locus_tag="POB92_000855"
     CDS             3130..6159
                     /locus_tag="POB92_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6578374.1"
                     /translation="MIKNKEINFEQDIEASLVQYGGWESVRFQDTHYDANIALDPTVL
                     LEFVKQTQPKNWKRYVSIYRTNAEQKFLKRLNEEITNHGILHVLRKGIRDRGVSFKVV
                     YFQPASSLNTENIKNYEANRFQCVRQFQYSNKYRNTIDMVLMINGIPLVALELKNQYT
                     GQNVEHARLQFEQDRDPKELIFQFNSRFLVYFAVDHFNVLMTTKLAKGNTYFLPFDQG
                     SNGPGRVGGAGNPSNPSGYPTSYLWENVLTSYQLLDIFERFMNLEEKKKLLIFPRYHQ
                     LDVVKKLISDTQHHGPGKNYLIQHSAGSGKSNSIAWLAYHLASLHSNEDQPIYSSVIV
                     VTDRTVLDRQLQNTLMSFEHTVGQVETIGDNKTSQDLKTAINDQKKIIITTLQKFPVI
                     YDEIDNTAGRSFAILVDEAHSSQTGSSAQKLKHALADKEASLKEYQEFEESVEDQSLD
                     DQDQIVQTLLSQGQHDNLSFFAFTATPKEKTIEMFGTKQADGSYHPFHIYSMRQAIEE
                     GFILNVLQNYMTYNTSYKIAKSIEEDPELPKTEAVRAIAKYQSLHPWVLRQKAEIMIE
                     QFRSVTQKAVQGRGKAMIVTASRLHAVRYMQEFKKYIKEKGYTDMDVLVAFSGTVEDD
                     GIEYTEPKMNLTHSGERIQENQLKEAFHSEDFNLLIVAEKYQTGFDEPLLHTMFVDKR
                     LRGVKAVQTLSRLNRTMPGKTDTFILDFVNTAEEIYEAFQPFYEATSLKEEININLIY
                     DTQSKLRKFHIYNDDDIQTVMKLNRQAKQKQNDQLLGRLSSVLRPVISRYSDLPEDVQ
                     YDFRVTLRNFNKWYNYIAQIDRTFDKELLEESIFTGFLLKFIPEKQRNYIDIEDKVKL
                     EYYKLQEDFKGDIALVNEDVENGQLAHPSEVNATVRPEEEYDSLNEIIHKINERFPND
                     FTQGDRVLVKNMRETLINNTDAKLVNMAQNNSEEMFANSLFQQQFQDFLIDQYNQGEA
                     AYSKMFSNNRDFYDTVYQLLAKDYYRWLRSQKADNFA"
     gene            complement(6537..7016)
                     /gene="rlmH"
                     /locus_tag="POB92_000856"
     CDS             complement(6537..7016)
                     /gene="rlmH"
                     /locus_tag="POB92_000856"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI6578375.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVTDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(7384..9702)
                     /gene="adsA"
                     /locus_tag="POB92_000857"
     CDS             complement(7384..9702)
                     /gene="adsA"
                     /locus_tag="POB92_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDI6578376.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDRQQVPPTKEAAHHYGEEAATNVSASAQGTADDTNNKVTSNAPTNKPTTAVSTTG
                     NKRHDVDAQQASTQKPTHTATFKLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALVQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFESIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDMTEPQRMLLGKPVASEQPAKGQQGSKGSESGKDTQPIGKEKVMNPA
                     KQPATGKVVLLPAHRGTVSSGTEGSDRALEGTAVSSKSGKQLANMSAPKGSAHEKQLP
                     KTGTDQSSSPAAMFVLVTGIGLIATVRRRKAS"
     gene            complement(9930..10730)
                     /locus_tag="POB92_000858"
     CDS             complement(9930..10730)
                     /locus_tag="POB92_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6578377.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(11119..11907)
                     /locus_tag="POB92_000859"
     CDS             complement(11119..11907)
                     /locus_tag="POB92_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDI6578378.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(11908..13242)
                     /locus_tag="POB92_000860"
     CDS             complement(11908..13242)
                     /locus_tag="POB92_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578379.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDYDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(13235..15061)
                     /gene="walK"
                     /locus_tag="POB92_000861"
     CDS             complement(13235..15061)
                     /gene="walK"
                     /locus_tag="POB92_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDI6578380.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(15074..15775)
                     /locus_tag="POB92_000862"
     CDS             complement(15074..15775)
                     /locus_tag="POB92_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6578381.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(16395..16467)
                     /locus_tag="POB92_000863"
     tRNA            complement(16395..16467)
                     /locus_tag="POB92_000863"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16432..16434),aa:Asp,seq:gtc)
     gene            complement(16475..16549)
                     /locus_tag="POB92_000864"
     tRNA            complement(16475..16549)
                     /locus_tag="POB92_000864"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16514..16516),aa:Glu,seq:ttc)
     gene            complement(16979..18262)
                     /locus_tag="POB92_000865"
     CDS             complement(16979..18262)
                     /locus_tag="POB92_000865"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI6578382.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(18541..19941)
                     /gene="dnaB"
                     /locus_tag="POB92_000866"
     CDS             complement(18541..19941)
                     /gene="dnaB"
                     /locus_tag="POB92_000866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6578383.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(19973..20419)
                     /gene="rplI"
                     /locus_tag="POB92_000867"
     CDS             complement(19973..20419)
                     /gene="rplI"
                     /locus_tag="POB92_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI6578384.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(20416..22383)
                     /gene="gdpP"
                     /locus_tag="POB92_000868"
     CDS             complement(20416..22383)
                     /gene="gdpP"
                     /locus_tag="POB92_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDI6578385.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(22398..23336)
                     /locus_tag="POB92_000869"
     CDS             complement(22398..23336)
                     /locus_tag="POB92_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDI6578386.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(23629..24597)
                     /locus_tag="POB92_000870"
     CDS             complement(23629..24597)
                     /locus_tag="POB92_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDI6578387.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(24657..24755)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(24959..25288)
                     /locus_tag="POB92_000871"
     CDS             complement(24959..25288)
                     /locus_tag="POB92_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDI6578388.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(25285..25980)
                     /locus_tag="POB92_000872"
     CDS             complement(25285..25980)
                     /locus_tag="POB92_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDI6578389.1"
                     /translation="MTTHLSFRQGVRECVPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     ALGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
BASE COUNT         8240 a         4920 c         4117 g         8843 t
ORIGIN      
        1 tttatctatt aaaggagata tacataatga gcgaatttaa aagacatcaa caaacatcaa
       61 ttaatgttca aaaacaagca aaccttattt ggaatgttgc agacatttta aggggtttat
      121 acaaacctca tgaatatgga aaggttatct taccaatgac tgtcattaaa cgtcttcacg
      181 atacattact aaaaacgcga gacaaagttc ttaagactgc agaaaatacc caatcaataa
      241 atgacgtaat gcgagaacgc cttttgaaga acgcttcagg gtattcattt tataatacaa
      301 gcttatatac ttttgaaaca ttactcgcag acccagcaaa tattgaatca aactttagag
      361 cttatctcaa tggtttctct gaaaacatgc aagatattct caataacttt aagttcgacg
      421 ttgaaattac gacaatggca gataatgacg tgctatttta tgtgattcaa gaatttaaca
      481 aagcagatgc atatctaggt ccagacaaaa tgacaagcac agatatggga tacgtgttcg
      541 aggaacttgt acgaaaattt tcggaaagct ataacgagga agcaggtgca cacttcacga
      601 gtcgagatat catttatctc atgactgatt tattgcttat cgaagataaa gatactttgt
      661 ttaaagaaca tgttttcaaa actgtctacg accaaacaat gggaacttcc caaatgctct
      721 cagcaatgac agaacgtatt cacgatatga atgatactgc cgaagtagct acctttggtc
      781 aagagctaaa tcctgaaact tatgcgattt cgaaagcaga tacaatgatt cgtgggggag
      841 atcctgaaaa catggcgctt ggaagtacat taacgagcga ccggtttgaa ggatttacat
      901 ttgattactg tatctccaac ccaccatttg gtgtcgattg gaaaaaggac caaaaagcag
      961 taaaagcaga acatgaactt ggtgaactcg gtcgctttgg tgttggattg cctcgagtta
     1021 gtgatggaca gttgttattt caactcaatg gtatttcaaa attaaaggaa acaggacgca
     1081 tggcaattat tcacaacggt tctgccttat tctctggaaa cccaggtgct ggcgagagtt
     1141 taattcgtca gtacgtaatt gagaatgatt ggttagaggg cataatccaa cttcctaacg
     1201 acttattcta caatacagga atttcaacgt atatttggat tattactaaa gataaatctc
     1261 cagaacgtca agggaaagta caactcgttg atgcgtccaa tatgtatgaa aaacgtcgta
     1321 agaatattgg tgaaaaacgc gtagatattt ctgaagcatg tcgagaaatg attgtgcaag
     1381 cttatggaga atttaatgat aaagaatact atctaggaga tggaactgta gagtcaaaaa
     1441 tacttaagaa tgaatcattt ggttttactc gagtgactat tgaacgaccg caacgagacg
     1501 aaaacggaga tatcgtttat aaaaagaatg gaagcaagag tgtagacacg aaccttagag
     1561 atacagaaga tattccctta acagaagata ttaatgaata ttttgaacgt gaaatattac
     1621 catttaatca agatgcatgg atggatcgta aaaaagataa gatagggtat gaaattccat
     1681 ttactagatt gttttacaaa tatacaccac cagaaccatc tgaagttatt tcagaacgta
     1741 ttaaacaact cgaagagtct attattaaaa acttccaagc attatctgga aaggaagtgg
     1801 atacaaatga gtagagaaat gaaatacagc ggaattgagt ggattggata tataccaaag
     1861 tattggacta ttactaagct taaaaatatt attgacttta taagtggtta tgcctttaaa
     1921 agtgaacttt tcacgatttc agataataat aaaaaagtaa taacaattaa gtcatttaat
     1981 accaaggaga ttattctcga taacctttct tactcaaacg aatctctcaa atttccaact
     2041 aaatatttat taaaaaataa tgatattctt tttgctatga gtgggggaac aactggaaaa
     2101 aatttattga tagaacaagt agacgattta tattatataa atcaaagagt gggaatcata
     2161 aggtcttctt ttagtaagtt tatttattat tatataaata ctggtttatt tagcgaatat
     2221 attaacttat tctcatcagg atcagctcaa cctaacatat ctgctacaga tatacaaaat
     2281 tttatcattg cgttacctga gaaagaaact attaaaaaaa ttgaaatata cattaattac
     2341 caattaaaaa ttatctcaaa tatcatagac acaacatatc aatctatcga agaacttaaa
     2401 aaatacaaac aatcactcat cacagaagcg gtgacaaaag gcatagaccc gaatgtggaa
     2461 atgaaagaaa gtggtaatga ttggattggt agtatccctt caaattggag tgttcgaaaa
     2521 attaaacatg atttcaattt aaaagggcgt attggatggc aaggtttaac ttctaacgaa
     2581 tatcagactg taggacccta tctcataacg ggaactgatt tcaaaaaagg cataatcagg
     2641 tgggattctt gcgttagaat tagtgaagaa cgttttgaag aagctccaga tattcatatt
     2701 aaagaaaatg atttattaat cactaaagac ggcacgattg gaaaagtcgc tttagcaaca
     2761 aatgttccaa aaaaagtttc attaaattca ggtgtactac tgattaggga aaaattaaaa
     2821 aatactatta ataaaaaatt tatgtattat aatttgttat caaatatgtt ttggaattgg
     2881 tacaattcta ataatcaagg tgctagcacc attaaacatc tatatcaagg tcaattctat
     2941 aattattctt atgcgatccc tctattgcat gaacaacaac aaatcgtcca atatctagat
     3001 gataaagttt ctactatcga taggctcatt gaagataaaa caaaagttat taaagagtta
     3061 gagaattata agaaatcatt aatttatgag tatgtcacag gtaaaaaaga agtgtaagga
     3121 gtgatatcag tgataaaaaa taaagaaatt aattttgaac aagatataga agcgagtcta
     3181 gttcagtacg ggggatggga gtcagtaaga tttcaagata ctcattacga tgcaaatatc
     3241 gcactcgatc caacagtatt attagaattt gtaaaacaaa ctcaaccaaa aaattggaag
     3301 cgatacgtat caatctatcg tacaaatgca gaacagaagt ttttaaaacg actaaatgaa
     3361 gaaatcacaa atcacggcat cttacatgtc cttcgtaaag gtatcagaga tcgaggagtc
     3421 tcttttaaag ttgtttactt tcaacctgca tcatctttaa atacagaaaa tataaaaaat
     3481 tatgaagcta atcgttttca atgtgtgcga caatttcaat atagtaataa gtatagaaac
     3541 actattgata tggtgttaat gattaatggg attcctcttg ttgcattaga acttaaaaat
     3601 caatatacag gccaaaatgt ggaacatgcg agattacaat ttgaacaaga tagagatcca
     3661 aaggaattaa tttttcaatt taactctcga ttcttagtct attttgcagt agatcatttt
     3721 aatgtgttaa tgacaacaaa actcgcaaaa gggaacactt actttctgcc attcgatcaa
     3781 ggatctaatg gtcctggtcg tgttggaggt gcaggaaatc catctaatcc atctggatac
     3841 cctacttctt atttatggga gaatgtactc acaagctatc aattattgga tatttttgaa
     3901 aggtttatga atttagaaga aaagaaaaaa ctactaattt ttccacgtta tcaccaactg
     3961 gatgttgtga aaaaattaat atcagatact cagcaccatg gtcctggaaa gaattatctc
     4021 attcaacatt ctgctggttc gggtaagtct aatagtattg cttggttggc ataccatctc
     4081 gcttccttgc atagtaatga ggatcaacca atctattcat cggtcattgt tgttacagac
     4141 cgtactgttt tagatcgcca acttcaaaat acattaatga gtttcgagca tactgttggg
     4201 caagtcgaaa ctattggaga taataaaact tctcaagatt taaaaacagc tataaatgac
     4261 caaaagaaaa ttattattac tacattacag aagttcccag taatttatga tgaaatagat
     4321 aatacagcag gtagaagttt tgcgattttg gtcgatgaag cgcattcttc tcaaacagga
     4381 agcagtgcac aaaaattaaa acatgcgtta gcagacaaag aagcttcttt aaaggaatac
     4441 caagaatttg aagaaagcgt tgaagatcaa tcgctagatg accaagatca aattgttcaa
     4501 acattactgt cccaaggtca acacgacaac ttaagttttt ttgcttttac agctacaccg
     4561 aaagagaaaa cgattgaaat gtttggaaca aaacaagcag atgggtccta tcatccattt
     4621 catatttata gtatgagaca agccattgaa gaaggattta ttctaaacgt tttacaaaat
     4681 tatatgacat acaacacttc ctataaaatt gcgaaatcga ttgaagaaga cccagaattg
     4741 ccaaaaacag aagcagttcg agctatcgct aaatatcaat ctcttcatcc ttgggtactt
     4801 agacaaaaag ccgagattat gattgaacaa tttagatcag tgacacaaaa agcagtccaa
     4861 ggtagaggaa aggcaatgat tgtgacagct tcacgactcc acgccgtacg gtatatgcaa
     4921 gagttcaaaa agtacatcaa agaaaaagga tacacggata tggatgtgtt agtcgctttt
     4981 tcaggtactg tagaagatga tggaattgaa tatacagagc cgaagatgaa cttaacacat
     5041 tctggagaac gcatccaaga aaatcaactg aaagaagcat ttcatagtga agactttaat
     5101 ttattaattg tcgcagaaaa ataccaaacg ggatttgacg aacctttact tcatacaatg
     5161 tttgtcgata agagattacg cggagtaaag gcagtccaaa cattgtctcg cctcaatcgt
     5221 actatgcctg gaaaaacaga tacttttatt cttgattttg taaatacagc agaagaaata
     5281 tatgaagcat tccaaccttt ttacgaagca acttctctaa aagaagaaat taatattaat
     5341 ttaatttatg atacacagag taaactacgt aaattccata tatacaatga tgatgatatt
     5401 caaacagtaa tgaaattaaa tcgtcaagca aaacaaaagc aaaatgatca attacttggt
     5461 cgattgagta gcgtgttacg ccccgttatt agtagataca gtgacctacc agaagatgtt
     5521 caatacgatt tcagagttac actaaggaac ttcaacaaat ggtataacta tatcgctcaa
     5581 attgatcgta cttttgataa agagttacta gaagaaagta ttttcactgg tttcttgtta
     5641 aaatttattc ctgaaaaaca acgcaactat atcgatattg aagacaaagt aaagttagaa
     5701 tactataaat tgcaggaaga tttcaaagga gatatagcac tcgtcaatga agatgtagaa
     5761 aatgggcaat tggctcatcc ttcagaagtg aatgcaactg ttagacctga agaagaatac
     5821 gattctttaa atgaaattat acacaaaatc aatgaacgtt ttccaaatga ttttactcaa
     5881 ggcgatagag tactcgttaa aaacatgcga gaaacattga taaacaatac agatgcgaaa
     5941 cttgtaaata tggctcaaaa caacagcgaa gaaatgtttg cgaatagttt attccaacaa
     6001 caattccaag acttcttgat tgatcaatat aatcaaggag aagctgcata ctctaaaatg
     6061 tttagcaata atagagactt ttatgacact gtctatcaac tactagcaaa agattattat
     6121 agatggctac gatcacaaaa agcagataat tttgcttaaa ttaattattt ttaacctaac
     6181 ttaaagatta ctcgcagtct ttaagttagt ttttgctaaa atttttaatt tagctatggt
     6241 ctctcatcct aatttcactt tcatacgata tttttttaat atttagttat tctaaagttt
     6301 ctaaattctt ataggatttt taaaagttta tctttacttg catgaaaaag caaatcacct
     6361 acatgctcta attgtaatct actcgataaa tgccccgcat ctccatacat ctcaataaat
     6421 ccctataatt ccaattattg cactaactat ttcacttacc attgttactc acttctgatt
     6481 ttcaaataaa ttaaaggatt ctaatatcta attaaatttt tcaagaaaaa accgcatcat
     6541 ttatgataag cttctccacg cataatctta aatgctctgt acacttgttc aattaacaca
     6601 acccgcatca tttgatgtgg aaatgtcatt ttgctgaatg atagtgcgta gttactacgt
     6661 tgtaagacgt ccttgtgcag gccgtttgat ccgccaatga cgaatacaaa gtcgctttgc
     6721 ccttgggtca tgcgttggtt caattcttgg gccaatcctt cggaagatag catctttcct
     6781 tgtatttcta atgtaatgac tgtggattgt ggtttgattt tggctagtat tcgttggcct
     6841 tctttttctt ttacttgctc gatttctttg tcgctcatat tttctggtgc tttttcgtct
     6901 gtaacttcta tgatgtctat cttggtgtat gggcctaaac gtttttcata ttctgctatg
     6961 gcttgcttcc aatatttctc ttttagtttc cctacagcta aaatggtgat tttcatgtcg
     7021 tttggtcctc caaattgtta tcaactttcc agttatccac aagttattaa cttgttcaca
     7081 cttttccctc ttattatacc aagttttttt gcagtttttg atatttttct gacattcatg
     7141 cccaattttc agtgtataac ttgtgtattt gtggataact ctgtgggcat tgtgggtatt
     7201 tctctttaaa ttgtacacaa ttctggataa ctatccccat ttctgtggat aaataccttg
     7261 atgtcttatt acttatcccc gtttttatat cccatattta tcaacggtta tagctttttt
     7321 ccataaaaaa acactaccgt acctcttatt taagaaatac agtagtattg ttttcaatat
     7381 attttagcta gcttttctac gtcgtacagt cgcgattaaa cctatacctg ttactaatac
     7441 aaacatcgct gctgggcttg aactttgatc agttccagtt tttggtaact gtttctcatg
     7501 tgcgctacct ttaggcgctg acatgttagc caattgtttc ccactcttac ttgatacagc
     7561 agttccttct aatgcgcgat cagaaccttc tgtaccgcta ctaacagttc ctctatgcgc
     7621 tggtaacaac acaactttac ctgtcgctgg ttgtttcgct ggattcatca ctttctcttt
     7681 accaattggt tgtgtatctt taccagactc actacctttg ctaccttgtt gtcctttagc
     7741 tggttgttca cttgctactg gtttacctaa taacatacgt tgtggttctg tcatatcata
     7801 cttagctaag ttagctgttt ttaaataact tgctagtact tgatctaatg aaataccttc
     7861 ttctctaggg ccaccgaaca tactatatcc gtctccacct gatgctgtga agtcattcat
     7921 cgttacatga tatacacgtt ttaaatcaat actttcaaac ttacctgtct ctttatttaa
     7981 aatttgaata gcattaattc gtttgccaga cggtttattc atatcatagt aaacacgaat
     8041 tgaatcagag atatgtaata aaccgccatt cgctgttaac actgtcttac cgtctttttg
     8101 tgttgttggt gcacctaaac tatgttcgaa agccgtccag acgtctgaac cttttacatc
     8161 aatttgcgca atcgtatttc caaatggtaa tactgagatt aaatcatagc gtgtcacctt
     8221 accttttgcg atagaggcac gaatacctcc accatttgtc acggcaaagt cagtcttttt
     8281 agagaaattc ttaacgccat atgcttccat agcatctgca atcgcgtttc ctaaatttgt
     8341 ttcacgcgtt ctaacgtcat ctctttctcc tttgaaatca atcgtattat ttggaataat
     8401 aacctctgct gtttgtgctc taaatgtttg atcagcttga ttaatttgtt cagctaatgc
     8461 tttgttcggt gttacatttt caacgtcttt aacattaatc aatgacggct taatattcga
     8521 tacttctcca ttgcgataat taaatgtaat cttaccgata ttcgcaagtg ctgtacctgt
     8581 ttgtactaat gcatcattgt tataaatttg accattttga agtacggtat gtgaatgacc
     8641 gtcaatcact gtaatacgtt tcttcaattg tggattttga cttaattgtt tcactaagta
     8701 atcgccacgc catgtttctt gtgttgaagg atcaatacct aaatgagata taacaacaaa
     8761 tgtatctaca tctttataaa tacgcatcat ttccgctgtc acactttgta atggatctct
     8821 aaattcaaca cctttaatgc cttcaggtct tgttttcgtc tttgtttctg gtgtcgttac
     8881 gccaataatt ccataacgaa taccattttt cgttacaatc gttgaaggct taaacgcgcg
     8941 ttttccatct ttataaacgt tagtacttag catcgggaag tctaacatac cctctaactt
     9001 tttcaactga tcgtatccaa agtcaaattc atggttaccg actgccatag catcataacc
     9061 tactgcattc attgctttag ccatttcttc acctttagat tgatttgaaa gtggtaaacc
     9121 ttggaaggcg tctcctgcgt ctaacattaa atcaggcttt tcttgttctt ttactgtttt
     9181 taatttagcc ataccgatga cacgcccttt ttcttcggct agtcggccat ggatatcatt
     9241 tgtatgtaat attttatgtg ttgttgtttg tggtacatta gcagcaaaca ttcgtggtga
     9301 aagtgatgct gttttagcat ttgataattt gaatgttgct gtgtgagttg gtttttgtgt
     9361 tgaggcttgt tgtgcatcta catcgtgtct tttgtttcct gttgttgaaa ctgctgtagt
     9421 tggtttgtta gttggtgcgt tggatgttac tttgttgttt gtatcatcag ctgttccctg
     9481 cgctgatgct gatacgttgg ttgccgcttc ttcaccataa tgatgagccg cttcctttgt
     9541 tggcggtact tgttgcctat ctgttgttgc tttgtctatc gttgttactg catgtgcttt
     9601 cattggtgta tgctgctcag ccgcgttcga gacttgccat aatcccatca cactaaaaag
     9661 caaaacgagc catacacttg ttttaagtaa taaagctttc atttcgttcc ctccattaac
     9721 tgtcatacac caatatatta tcataatcct tatattaaaa tgattgattt tatgtaaatt
     9781 atttataaat tacatttaat atcgaattat tttacacatt tcaatcaagc cggttcaatc
     9841 taacaacagc catttcccac caataacata aaaaagcacc cgataaagtc gtctcacagc
     9901 agtgccgaac tttatagggt gactctcatt tatattgtat atattggcgt tggaatagct
     9961 ttatccgtat cacatagcaa tacttctttt tccgtatcaa tatcgtgttc gttcaatact
    10021 tgaccaacac tcatacgcgc caaatctttc atattattat cttgtgataa atgtgatagg
    10081 taaatacgtt tcgtgttgcc tgtaatcaca tccgtcatcg catggcccgc atcctcatta
    10141 gatacatgtc ccatatcgcc taaaatacgt tgtttcgtct tccatggata acgacacatt
    10201 ctcaacatat cgacgtcatg attactctca aaaataaacg catcgctgcc acgtatcata
    10261 cctttcatac gatccgacac atatcccgta tccgttaaaa tcgtaaactt cttatagtta
    10321 ttatggaaaa tataaaattg cggatctatc gcatcatgtg acacgttaaa cgattcaaca
    10381 tcgaaacctg caatagattt cgtttcataa ggattaaaaa tgaatttctg atccataggg
    10441 atgcgactat ctttcttttc aatagcttgc caagtctttt cattcgcata aattggcaat
    10501 tgatatttac gcgctaaaac acctaaacct ttaatatggt caatatgttc atgggttact
    10561 aaaataccat ttaaatcttg aatattacgg tcaatttgac taaacaattc ttccattttc
    10621 ttgccagtca aaccaacatc aactaataga ctaccttttt cattttctac aaaagtggcg
    10681 ttacctgtac taccacttgc taatacactc atgcgtatca agcggctcat cctttctatt
    10741 taagcagtca atctctctat atgactgcaa tatacatcag tgcaaatata cgattttccc
    10801 tacagaaact atcagtatat tagctgtttt gtataccttt cctattatgt aactttagtt
    10861 catcgaccgg tctcttataa ttaaattttg ataatcacgt gcaatgaatg tcctaattat
    10921 tataacaatg attagacaaa tactaaagtt tttaaaataa cagttacact aattttacaa
    10981 tgcggtacta cttaaaatat gaataaatgc gcacgttgga gacacagaaa cgtcagccat
    11041 aataaaccgt accagcaaaa agctagcacc gcttctcaca tatgatgagc ttgcaatgct
    11101 agcttattac gattcatatt aatgattaat aattttaggg ttattatttg tcgcttcgac
    11161 atagtaagta ttcgttttgt ccttaccgtc atgcttcact ttaatttccc agtttggttg
    11221 taacaattga acattcgttt cattcacgac tgaatagtaa ccaagtctag cattaatgac
    11281 ttcatcacct tttttcaagt aacgattata atataatgcc tcgattgctt ttctcacacc
    11341 aatcacttga tgcttcttat ctgcaccatc tgtgggctta atgtcatcca tcattgattg
    11401 tttataacta gtcgctttgt tatcttctat attaaagttc aacatcgctt tactattgtt
    11461 caaaatcggg aaattatcat acgtttgttc gtattttaca gaaccagaac taatttcact
    11521 taattgatat tctgaacctt tgaacacacg tttgttaaca taatctttca aatcttttaa
    11581 gttattgtca ctgaccttta ccgattgact aatgttcgca tttaataatt ttccaccatc
    11641 tgatgtggtc aaatccgaat cgcctttagc tttagaacta aagtcttttg atcgccctgt
    11701 aatttgctct aattttatac ctttaactga tttattcaat atactagccg gtactttaat
    11761 ttcttcttgc tgaaaattaa cttcattgtt actctcaact tcattaatgt gtgagcgatt
    11821 gactttatta acataaatcg atactaacac gatgttgaca agaataaaca cgaaaatgaa
    11881 aagtgtcttt gtcagtttcc agttcattta ttcaagcctc ccatcgttat aaacatacca
    11941 ttcgccatca tattctacat accaacgcgg tacgagttca ctgttaatct gcacttcaat
    12001 gtgattatga tctgagttat cctgcatttc gtaaccgata gcgatgtttg ttactttttc
    12061 aaaattaata tcactattgt tcgctaagct tgaacgtaca gactctaatt tcggcaacgt
    12121 tttattatct tcactgttta aaacaacgtc ggtgcgtaat aacgaacgac gataatcaaa
    12181 gacgcctttt tcaccccaag tgacttgaat ttgattagag ccttctttat taaacgttgg
    12241 ataaccatta aggaaacgtt gatatgttaa ctcgcctgac tgattattcg tactaaacaa
    12301 tctgaagtct tcgtttaaga aaccaccatg accattaata aaatcaaagg ttcctggaat
    12361 cgtttcttcc attttgctgg aactcgcttc atcttcggac aagtttttat aatgatattt
    12421 ttcattttta tcgttatagt ttgcgacacc tgtattattg ttgtaggttg taacaccact
    12481 ctttgaacta cgaacgatgg ttgaatcgtc aaatagtata gcattcattt tctcaacact
    12541 aatcgtgtta aataccatgc gatatgtttt taacttttca ggtttacttg gtgcaaaaac
    12601 atgcgtcgta cgatcaatcg tatctttgtt tgtgatgata tctgtgtatg gttgcatatc
    12661 ttttttcact gctgctaatg catctaaaaa atgatcattt ttcgttgtag ttgttaattt
    12721 tacgtaatcg tggcgatctt tgcttatagc ataaagcacg atattatcat cagcatcata
    12781 atctatgacc aaacgattga aattgaaatg atttggtact ttcgcattca tgttcagtac
    12841 ttggccaaga tatgttgata acggtaaatc atacgtaaaa tcgaataatg taaaatcact
    12901 gctcaaatca ggaatcatca agttatgatc ccgacgaaca tgttccacgg acttcacttc
    12961 tttatttttc aaaggtttcg tcagtttatt cacattagat accgtcgcga ttgtaccttc
    13021 tggatgatca tttttcgaat gaataatctg aaatggcgta atagttgtat ccattttggc
    13081 tgtcataggt gtcgttaaag gtttcgtttc actcttctta ctatctgtat tgtcgacatt
    13141 tgcaatatca ggagaaaagt tccataccat atatgtcaat acgacactca tcaagacgag
    13201 tagcgctaaa atgacagatt taatatgttc cttattattc atcccaatca ccgtcttcaa
    13261 tgacttcaca tggaagtgtg ataaagatag atgtaccttg accttctaca ctgtttgccc
    13321 aaatacgacc attgtgcgct tccacaatct ctttcgaaat ggctagtcct aatccagtac
    13381 cacccatttt acgcgtacgc gccttatcta cacgatagaa tcggtcgaat atcttatcga
    13441 ctttattgat aggaataccg atgccattat ctttaatacg aatcgtcatt cgattataaa
    13501 gtggattttg tttcacgtgg aactcgacac gtttatcgcc tctagaatat ttcatcgcat
    13561 ttgtaatgac attatcaaat acttgcgtca ttttatcagg atcaaattct gtgaaaatcg
    13621 tctttttcgg aatatctcga ataaatgttg tatctttcgc agacatttca tgtcgattaa
    13681 taattttatt aatgaacatg ttaaagtcga taatttcttt gttgatttga tcagactcat
    13741 tatccatttt agataactgt agcaagtcat tgaccagtcg aatcattcgt tctgtttctt
    13801 cacgggtaac agataaaaat tgtggcgcaa gttcctcatc tttccatgca ccttcttcaa
    13861 gtgcttcaat gtaactattc atagaagtta aaggtgtacg taactcatgt gatacattgg
    13921 caacaaattc acgacgctca cgttcaactt gttgttgttc agtaacgtca tgtaacacag
    13981 cgatataacc agttacaaat cctgtttcct gcacaatcgt actaaagtta acacgtgcga
    14041 ttagaccttc ttcttcattt aaatctaata agaaactatc attattctct tgaatttctt
    14101 caagtttaaa ttcatcttca agacttaata cacttaacat gtaatatcca atgatgtctt
    14161 ctttcgccat accaagcatc ttgagtgcca tatcattgac gatacgaata cgtccacggc
    14221 ggtctgttgc aataatacca tcactcatat gggtgataac tgagtccagt ctacgtttct
    14281 cactttcagt attagcctgc gcttcttgta cacgtttaga caagttatta aatgctaaag
    14341 ctaattcgcc aatttcatca ttaccataaa tcttcacacg ttgcgtatag ttacctctgg
    14401 acatttcaac cgtctggtta cgcatatcgg tgattggttt ggtaatcgtt cgcgctataa
    14461 agaatcctag gatgactgtg attaataatg aaatagctgt accaacaatg aatatttgat
    14521 ttatattatt taattggtta taaacgtcat taatttttga ttcgatataa atattaccaa
    14581 ttaccttttt atcgacttta actgggatat tatataccca gacacggtcc ttaccaccgc
    14641 cataatcttt taaaattaaa tgatcgtttg attgtcctag tgatagtgct ttttggacag
    14701 aactatcatt cgctttttga ttgattagac tacggttaga ctgcttcgtc gtcgcaataa
    14761 taatttggtc tttatctata aaacgaattt ctccaatttc ttgacggttg gcatactcac
    14821 ttaataaatt ttgaatatct ttttgtgcat ttacggagcc cttttcgtca tatacttttt
    14881 caatactaat ttctaattgt ttagcgtact gcgtaatatt cttcttaaaa ttatcaagca
    14941 gctctttttc aagattattt gtaaaataca gcccgataat ttgcatacca atgataatca
    15001 gtaatacata aacaattaca agtttagtat gaagggattg tagttgtttt agccacttca
    15061 ttcgtttcga cctctactca tgttgttgga ggaaatatcc aacgcctcta cgcgtcacaa
    15121 tatattcagg atgtgacgga tcatcttcaa tcttttcacg taaacgacga atcgttacat
    15181 cgaccgtacg tacatcgcca aagtaatcat agccccatac tgtttgtaat aaatgttcac
    15241 gtgtcattac ttgtcccata tgttttgata aataatggaa caattcaaat tcacgatgtg
    15301 ttaattcaat atcttcgcca cgttttttaa tagaatatgc gtctggataa atcacaatat
    15361 ctttaattgt gatttcattc gttacatttc cagtgtcttg tgctggttgt gagtaatgac
    15421 gacgtaagtt cgctttcaca cgtgcgatta attcacgcgt actaaacggt ttcgttacat
    15481 agtcatctgc acctagttct aaaccaagca ctttatcaat ttctgaatct ttagcagtaa
    15541 gcattataat tggcatttcg tattttttgc gcacttcacg acatacttcc ataccatcac
    15601 gaccaggtaa catgatatct agtaatacga tgtctggttc ttcttcataa attaagtcga
    15661 ctgcatcatt accatcgtat gcacagtaca catcgtatcc ttcttttttt aagttaaatt
    15721 ctaaaatatc agcaatcggt ttttcatcat caactacaac aacttttcta gccatttgca
    15781 taaacctctt ttcttaaatc attaatatta ttttatcatt ttttgttctt tatcgtttat
    15841 gtttgtgtaa aaaatcacag gatgtcgctt actatttttg ttattgtgcg cattttccgt
    15901 cttataacgt atagtaacct attatataat ttaccattta taagctatta attctatttt
    15961 ccaatataaa cttatatatt actatttttt attagtttct cacggcggat tgtgacaatg
    16021 atatttaaca caatgatgag gtctctattc caataataaa attaggataa tggcaacttc
    16081 tagttgcacg tcattgctca gaaaataaat atcattattc gttattcgtt actttaacaa
    16141 ttgatgttat ggaggactgg tggcatatca caacgaagaa atatgttgac agtagcatat
    16201 gtttgtacgt ctaataaaga ttttattgtc tacgtttatc tttcatatcc aaccactaag
    16261 ttacttgagg ttcatacatt ttaaagaaaa tggacgaaca gataaatcaa tatgattatc
    16321 attcgtccat atatctttta ttaatataat attaaacgca aaaaaatgcc tatcctcttg
    16381 gataggcatt tgtacggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
    16441 gcgtgttaac cgctacacta cgagacctaa aaaatggtga ctcctacggg actcgaaccc
    16501 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caaggagccg ttcttaagca
    16561 caagatagat tatagcacag ccaatcttgc catgacaaga tattttttac ataattttgc
    16621 tattattttt tgaagaatgt tataagtgaa aaattgcatt ttattggcag tctatttatt
    16681 ataataatta cgctattttt gaaatttttc tattttaggt gctttcattt acacttacgt
    16741 ctactctatt ttaatagcta ttttcgacat cagacttctt acattcaagt atttcatctt
    16801 aattttcgtt aaatatcatc taatcttcta aattatgact agcaaatatc gcctttacgt
    16861 tagtattttt tatctaaata atcgaatcga tacagaagca agcataacaa aagcctccta
    16921 ccaataaaga tagaaggctc atgtatttat aatcattgtg tatgacttat ataaagttct
    16981 accacaattc ttttaatagg tttgtttgtt ctctatctgg accaactgag aagatagaaa
    17041 tttgtacatt acataattct gaaatacgct ctaaatattt acgtgcattt tcaggtaatt
    17101 cttctaaagt acgcacattt gttacgtctt ctgtccaacc tggtaactct tcaaagattg
    17161 gtttacaacg ttttaattga tctaagtttg ctgggtactc agtaatttct ttaccgtcta
    17221 attcataagc tgtacagatt ttcactgtgt ctaggcctgt taaaacatcg attgagttaa
    17281 tagataaatc tgtaatacca cttacacgac gagagtgacg taatacaact gaatcaaacc
    17341 aacctacacg acgtggacgt cctgttgttg taccgtattc acgaccaacc tctctaatat
    17401 gatgtccatc ttcatcgaat aattcagtag ggaatggacc atcaccaaca cgtgatgtat
    17461 aagctttaca tacaccaatt acctttgaaa cgaatgtagg acctacacct gtaccaacag
    17521 taacgttacc tgcaattgga ttacttgatg taacgaatgg atatgtacca tggtcgatat
    17581 ctaacattac accttgcgca ccttcgaaaa gtaccttttc atctgctaca aatgcatcgt
    17641 ctaagatttt tgatgtgtct gttacaaatt cttttaaacg ttgacctgca gcatagtatt
    17701 cttcaaagat atcatcaaat gatggacatg tttcgttaaa catacctttg aaatatgctt
    17761 gtttatattc aatgtttgat tttaataatc tttcgaatgt ttctttttca agtaaatctg
    17821 ccatacgaat accgatacgt tgaactttgt ctacatatgc tggaccgata ccttttttag
    17881 ttgtaccaat cttattgtca ccgcgtaaac gttcttcata ttcatcttgt gctaagtgat
    17941 atggtaaaat cacttgcgca cgattagata tacgtaaatt acttgtagga atgccacgtt
    18001 catttaatcc gtctaattct ttcaatagtg caactggatc aacaacgaca ccgttaccga
    18061 ttaccgctaa tttgtctttg taaaagatac cagatggtac taaatgtaat ttatatgttt
    18121 ctccgccaaa ttgaatggta tggcctgcat tattaccacc tgaaaaacgc gcgataacat
    18181 ctgactgttc tgccaagaaa tccgttattt ttccttttcc ttcgtctccc cattgtgtcc
    18241 caactactac gattgatgac atgtgagcac ctccaagttt ttctcattta accatttgat
    18301 attctaccaa tatcgagacc ataatgcaaa taaaaatcga acattgattt tcagcctttt
    18361 taaaagaaaa atcattttaa caaatcgtct tcaacgtcct aaaaaccctt taacagaggc
    18421 gttgacagta ctaatccatc actttcaata cacaaactta acatatatca acccgttcag
    18481 aacaaaataa aaaccgtaca attttatcat cgcaatgatt attgtacgga aaaacttttt
    18541 ttacatcata tcagcatgtg cataatcgat atctgtaaat ttattatatt gtttcataaa
    18601 gtgtaactta actgtgcctg ttggaccgtt acgttgctta gcaatgatga tttctatttc
    18661 accgttttca tcattcgttt gtggctcaaa accaccatca tcgtcatcat cttcatcgcc
    18721 gccacggtta tagtaatcat cacggtataa gaacgcaacg atatcagcat cttgctcaat
    18781 cgaaccagat tcacgaatat cactcatcat tggacgttta tcttgtcgtt gttcaacacc
    18841 acgagataac tgacttaatg cgataactgg acattctaat tcacgggcta atgcttttaa
    18901 tgtacgagag atttcagaaa cttcctgttg tctgttatcg gacgcacgtg aaccactacc
    18961 ttgaatcaac tgtaagtagt caatcacaat catgtctaag ccatgttctt gctttaatcg
    19021 acgacattta gaacgtaaat cattaattcg aatacccggt gtatcatcaa taaaaatctt
    19081 cgtacgtgat aatttaccta ccgctatagt aaaacgactc caatcttcct cagtcatagt
    19141 acccgttctt aagcggtttg agtcaacatt tccagaacta caaatcatac gtgtggctaa
    19201 ctgatcagca cccatctcta gcgagaaaat accaactgta tacatatctt catgcgttgc
    19261 aactttttgt gcaatattaa gtgcgaacgc agtcttacct acagatggac gcgctgcaag
    19321 gataattaaa tcatttcggt tgaaccctgc tgtcatttgg tctaaatctc gatatcctgt
    19381 aggtatacct ggtgtttgac cactattttg atcaagctct tcagctgttt catacacttg
    19441 tcctaagacg tctcgaatgt ctttaaagcc atcgctttca cgagaagatg atagctctaa
    19501 aattcgacgt tctgcatcac ttaaaatcgc atctagttca agttcatcat tatatccatc
    19561 attggcaata ctatctgcag tttgaatcaa tctacgtttt aatgcatgct tagaaacgat
    19621 atcagtataa tactgaacat ttcgcgtcgt tggtacattt gtagataact ctgcaagata
    19681 ttgcgggcca cccgcttcat tcaacgtacc ttccgtcgat aattgatcca tcaatgttac
    19741 aacatcaatt tctttattat cttcatttaa gtgcatcatt gcacggaaaa tatgttgatg
    19801 ggcaccccta taaaacgact caggaagcaa aacttcctga gtagtattaa tcaattctgg
    19861 atctataata attgaaccta agacagactg ttcagcttca ttgttatgcg gcatttgatt
    19921 ttgctcatac attctatcca tgaatggtta cacctcttat ttcaatccaa ctttattgtt
    19981 caactgtgtg tacgcgaatt gtaccttcaa cttctttatc taatttaaca ggtacattcg
    20041 tatatcctag ggaatgaatt ccatttggta aatccatttt acgtttatca attttaatat
    20101 catgttgtgc ttttagtgct tcggcaattt gttttgtact tactgaccca aacaatttac
    20161 caccttcacc agtttttgct gatacttcaa cttcaatgtt tgataacgtt tcttttaatg
    20221 ctttagcatc ttcaatttct tgttggcgtt cttgttttgc acgttttttc tgtaactcta
    20281 attgtttaag gttacctggt gttgcttcta cagcataatt ctttttcaat aagaagttat
    20341 ttgcataacc tactggtact tctttaactt cacctttttt acctttacct ttaacatctt
    20401 gtgtaaaaat tactttcatg catcttcact cctacttaat tgttctgtaa ttgcttgttg
    20461 taattgtgct atcgcctctt cgactgtcac acctttaagt tgtgttgccg cattggttaa
    20521 atgtccaccg ccaccaagtg cttccattgt taactggaca tttactgaac cgagtgaacg
    20581 cgcagatata ccaatcagat tatcttcacg tctcgcaaca acatatgatg cttcaatacc
    20641 ttctaaactt aacagttcat ctgctgcttg tgcaactgtt actggatgat aaattttatc
    20701 gtctgaacca tgcgcaattg ctatgccatt atcttcaact tttacagttc gaattaattc
    20761 agatcgatta atgtaagtat ccacatcatc ttttaagaaa tgttgcgtta aaatcgtatc
    20821 tgcaccatgt gcacgtaaat aactcgctgc atcgaatgtt cttgatcctg ttcgtaatgt
    20881 aaagtttctt gtatctacaa taatacctgc atacatcact gttgattcaa gacgtgttaa
    20941 acgttgttct gttggttgat attccagtaa ctctgttacc aattcagctg tcgaacttgc
    21001 gtatggttcc atatatatca acaatggatt agagatgaag ctttcaccac gtctatgatg
    21061 atcgataaca actttacggt ttgctttatt taagacattt tcatctaaaa ccagttccgg
    21121 tttatgcgta tcaacaatca ctacggttgt cttagatgtc atcatatccc aagcatcatc
    21181 tgatgtaata aatcgctctc ttaactctgg ctttttatca atttcgttca tcacgcgtcg
    21241 taatgttgga tcaatgtcag tctcatttaa tacgatgtat gcttctaaat tattcatcat
    21301 tgcaaatcta gacacaccga ttgctgcacc aattgcatct aagtcaggac gtttatgtcc
    21361 catgataatg actttgtcac cctctgcaag gatatctttt aacgcatgtg agataacacg
    21421 cgctcttaca cgagtacgtt tctccatcgg gtcagtctta ccgccataga agcgcacatt
    21481 accattaata cttttaattg caacttggtc gccaccgcgt cctaatgcta agtctaggcc
    21541 tgattgtgat aattcaccta aatcgattaa attttcagta ccttcaccaa caccaatact
    21601 taatgttaat tgggcacgat aaccaacact tttttcacgt aattgactca agatatcaaa
    21661 tttagattct tctaagtcag ctaatatttt ttgatttaaa taggctacga attgatcgga
    21721 actatacctt ttaaagaata tattatactc agttgcccat cgactaataa cacgcgttac
    21781 cattgaattg atttccgaac gctgcgtatc attcatattt tgagtaatct catcgtagtt
    21841 atctaaaaat aatgtcgcaa tgattggttt agaattttca tatagttcat ttgtttgtac
    21901 ttgttcagtt atatcaaaga aatagaggca gtgatcattc tcagaataac gtacttggaa
    21961 atgatactga ttatattcta tttcaacgga tttcactcta tctaattgct ttaaaatgtt
    22021 tggaaatact tcatttacag attcagaaat gacatttgct tccatatgat ctgtcataaa
    22081 ttggttaacc cattcgatgt gatcattttc atctaaaaca atgataccaa ttggtaaatg
    22141 tttgattgct ttattatttg ttgttgaaat ttgagcactc aaaccatcta cataactatc
    22201 cattttcatt aaagcttgtc tgaataaaac gatgctaaca ataatcatca cgacaagaac
    22261 gatagatgca attagtgcta taagactatt aaagataaac catacaccca ttaaaacaat
    22321 tgctgtgatg atcatgatga caaatggtat tagtaaagct ttcttagtgg actgccgatt
    22381 cattattcca cctctattca ctttttagaa ttatttttca tgattcgctt caaattcaaa
    22441 cttaaatcga taacaccaag tagtcctaca atatgtgtcg taggtgtcag tattgtaccg
    22501 ataatcaata gtaaaatcgt tactgcattc ggcaaacctt tcgctttacc aaagaaatga
    22561 ataacactta aaccttgaat atacattact aatgataaca caagttggaa gtttaaaaga
    22621 atgctctgga acacactcgg ttgacctgta aataataaac atatgataac aataatgtat
    22681 atccataata aaataccgct catttgccac gcgaaaagtg gcttaaatac aggtgtagcg
    22741 attttaaatt ttcgtaaaat cggaaatgta acgattaagt taattaagac gattaaaaat
    22801 gtaatgataa tgatgaaacc tggtaattga acggtcgctt gtctaaaccc ttcttctaat
    22861 atttgggtca tattcgcatc ggcaccgctc atcgtaatcg cttcatgtaa tgtttgcttg
    22921 aaaggtttta ctatgctcgc tgatggtgga attcttccga atgtttgtag taacataaaa
    22981 gcgattaatg aaattaagct catcgctact gttgttacgt ataatattct ttctttagac
    23041 gttctttctt tgagcaattg accaataatt aaacttgcaa ttaagactaa tatgatggca
    23101 cttaaaacga aagtattacc taaaacagtt gttataatta ctgtaataag tgcactaatc
    23161 ccgaaagatt gtattgattt attccataaa acgatacctg gtatggttgc aaacaatgca
    23221 aatattagtc ctaaaccagg cactagatat aaagctaaag cgacaaatac caacgcaatc
    23281 gttgcaatta ttgttgcttt aggttgtatt tttgaaaaca cataagccac tcccatattt
    23341 ttaactatag ctattatttt aacctcttta atggaattta acaatttata gattgtatgc
    23401 ttctatttca tttaattgaa taataacttt catgttttat aagtaattaa catactcatt
    23461 tgaatcgctt ttgtgtgctt tcattttcaa catgattatt taatcccact acatagcaat
    23521 caagcttgat ttagatttac aattcattac cactctcatg tactctagat gtttttgaat
    23581 atgatagctg tgatttagtg gcttcattct ttgaaaatat atatattatt acttacgctt
    23641 aaaatgcttt aaatttaaga aatgatataa gttaggtgcc caggtactaa agtttagtag
    23701 gaatccatca tgcccaacat tatcaggcac gaagaaatga cgatgatatt taaaacgttc
    23761 acctaatgca cgaacttgat catccggata tagcaaatca tctatgaacc ccatcgttaa
    23821 cacttttgtt tctaaatttt taaaaacatg cgttacgtct gtgcgacctc ggtcaatgtt
    23881 gtgactatcc aatacatcta gcagtgtcag ataacaattc aaatcaaaat gttctttaaa
    23941 tttattacct tgatgttgtt ggtatgcgac tacttcatcc ggcgtgaaac gttcatcata
    24001 actttttgat gatcgatatg tcaaaaaacc taattggcgt gcaatactta gaccttcctt
    24061 accaccaaga tgaatggctt gccttgcaat ttcattgaaa gctctactat aagatgatgt
    24121 tcggcttgtt gcagcaagga taatggcttt atctacttca aactgttgat tgtaaagtag
    24181 ttccattgct tgcattcctc caaggcttcc ccctattaaa atattaatct tgtcataacc
    24241 aagggcttgt atacctcgtt cattcgctct gactatatct cttaatgtta attttttagg
    24301 aaaatgaggg tcgtttaaag gtgaacttga accgaaagga ctaccaataa catcaaatgt
    24361 taaaaattga taatcgtgaa tgggtatata tcccccatca ataatttctc gccaccaacc
    24421 cggataatca tctgttccat atgttaaatg attgccagtt aatgcatgac aaactacaac
    24481 taatggttgt ccatgataac cgacatgctc atatctcaaa cgcaagttat ctatgacttc
    24541 cccagattct gtaataaatt tccctaaatt taaagtatct actgtgtaat ttgtcattgt
    24601 tttttcctcc ttaaacaaaa aaacttctca ccctattgaa aagtaagaag tctttatact
    24661 tatcattcga gtaactcgtt ggttttagca ccgtgctata aagtcggttg ctgaagtatc
    24721 acagggccaa gtccctcaac ttctcacaat aagaaatatg aaattgttat gtgttagttg
    24781 agattcagtg atgaattact ttatcattta aaatgttgtt atcattgtca tgcgttagcc
    24841 aaatcgctta cgtatacacg attcccaatc ttaacataga cgatttgtat atcagaattt
    24901 tctgattact aacagtttac ctaagtttaa atatctgttc aatgattttc agttattttt
    24961 aaaagaaaaa tcgtaatgct gccatgataa caatcccact aataattgta atagttaaac
    25021 tacgcgtgat tatagataaa ataaccgtcg gaatgagcgc gataatgtaa gggatgttta
    25081 atgtataccc ctcaccatga ggcgtctgtt gaataatgct gtctatgaca agtgccgtaa
    25141 atagtgtgat tgggataaat gatagccatc gaaccacgac atcaggcaat tgcacttttg
    25201 aaatcatgat aaaaggtata attcgaatta atagcgttac gataccacac aataaaataa
    25261 gtattaacat gttcatatga gttatcatcg ttccatcatc actcctaacg ctgctgaaat
    25321 tgtggctgca attaatattg ctagatatga aggcataaac atacttagcg ataacatcat
    25381 tactatgacg gcaataatga gtactatgta aattcttaat cgcgatttag taattgattc
    25441 aaattgcgca atggccaaaa agataaacat agccgtgata gcaaaatcta accctaatgc
    25501 ttgtggattc gaaatatact cgccaaacaa tgcacctgct acacatgaaa ttgcccaaaa
    25561 taaatatgct gtgatattaa gaccatgcat ccaacgatca ttgatagctt ctccttttaa
    25621 ataaggtgta atggcgacgc caaacgtttc gtcagttact aatgaaccta atccaacacg
    25681 gttccaaaac ccatatgtct tgaagtttgg tgcaagcgac atacttaaaa ggaacattct
    25741 tgaatttacg ataaatacag ttagtacaat cgctgatatc ggtgtacctg ctataaacaa
    25801 cgcgcacata ataaattgcg cagcaccggc atatataaca agacataaca agacaatttc
    25861 taaaatacta aagttttgag acgaagccac aataccaaat gaaataccaa caccggcata
    25921 acccaataat gttgggacac actctcgcac gccttgtcta aaacttaaat gtgttgtcat
    25981 ctcaactacc tcctttgcca ctttatattt atataattac gaaaaaaata atatacctag
    26041 tctaatacag ttctatatca caagcaataa cgttagacac tggtttgtct gaagttagac
    26101 ataccggagt ctttaatcag
//
LOCUS       DAMZLE010000023        26010 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMZLE010000023 DAMZLE010000000
VERSION     DAMZLE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.023"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(93..995)
                     /locus_tag="POB92_000873"
     CDS             complement(93..995)
                     /locus_tag="POB92_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDI6578390.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(1161..4814)
                     /gene="addA"
                     /locus_tag="POB92_000874"
     CDS             complement(1161..4814)
                     /gene="addA"
                     /locus_tag="POB92_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDI6578391.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            complement(4815..8291)
                     /gene="addB"
                     /locus_tag="POB92_000875"
     CDS             complement(4815..8291)
                     /gene="addB"
                     /locus_tag="POB92_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDI6578392.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDVLQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVFLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYIAMTRARQDVTFSYRLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            complement(8451..9026)
                     /gene="lepB"
                     /locus_tag="POB92_000876"
     CDS             complement(8451..9026)
                     /gene="lepB"
                     /locus_tag="POB92_000876"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6578393.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            complement(9042..9566)
                     /gene="lepB"
                     /locus_tag="POB92_000877"
     CDS             complement(9042..9566)
                     /gene="lepB"
                     /locus_tag="POB92_000877"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6578394.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            complement(9571..10146)
                     /locus_tag="POB92_000878"
     CDS             complement(9571..10146)
                     /locus_tag="POB92_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6578395.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            complement(10471..11802)
                     /locus_tag="POB92_000879"
     CDS             complement(10471..11802)
                     /locus_tag="POB92_000879"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI6578396.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            12153..13358
                     /locus_tag="POB92_000880"
     CDS             12153..13358
                     /locus_tag="POB92_000880"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDI6578397.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDAADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNSM
                     LHGGYSNEQ"
     gene            13348..14727
                     /gene="argH"
                     /locus_tag="POB92_000881"
     CDS             13348..14727
                     /gene="argH"
                     /locus_tag="POB92_000881"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI6578398.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLINQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            14969..15898
                     /locus_tag="POB92_000882"
     CDS             14969..15898
                     /locus_tag="POB92_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6578399.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(16288..17532)
                     /locus_tag="POB92_000883"
     CDS             complement(16288..17532)
                     /locus_tag="POB92_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDI6578400.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(17641..18831)
                     /locus_tag="POB92_000884"
     CDS             complement(17641..18831)
                     /locus_tag="POB92_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDI6578401.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(19139..20266)
                     /locus_tag="POB92_000885"
     CDS             complement(19139..20266)
                     /locus_tag="POB92_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6578402.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPGGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(20628..21005)
                     /locus_tag="POB92_000886"
     CDS             complement(20628..21005)
                     /locus_tag="POB92_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDI6578403.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(21420..22013)
                     /locus_tag="POB92_000887"
     CDS             complement(21420..22013)
                     /locus_tag="POB92_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6578404.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            22077..22460
                     /locus_tag="POB92_000888"
     CDS             22077..22460
                     /locus_tag="POB92_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDI6578405.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            22591..24996
                     /gene="mnhA1"
                     /locus_tag="POB92_000889"
     CDS             22591..24996
                     /gene="mnhA1"
                     /locus_tag="POB92_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDI6578406.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            24989..25417
                     /gene="mnhB1"
                     /locus_tag="POB92_000890"
     CDS             24989..25417
                     /gene="mnhB1"
                     /locus_tag="POB92_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDI6578407.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            25417..25758
                     /gene="mnhC1"
                     /locus_tag="POB92_000891"
     CDS             25417..25758
                     /gene="mnhC1"
                     /locus_tag="POB92_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDI6578408.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            25751..>26010
                     /locus_tag="POB92_000892"
     CDS             25751..>26010
                     /locus_tag="POB92_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="subunit D of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; contains an oxidoreductase domain; catalyzes the
                     transfer of electrons from NADH to ubiquinone; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HDI6578409.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIIT"
BASE COUNT         8285 a         4675 c         3893 g         9157 t
ORIGIN      
        1 cattataaat aatttatctt taacaccttt ttagaaaagc aagaaaaaac taaccaatct
       61 atataaagac tggttagctt tttaaatgat aattatttag cgatataagt tgtcagcgtt
      121 ccaatattat caatagtcac tttaacttca tcacctggtt gtaaaaattt aggtggttgc
      181 atacctgcac caacgcctgc tggtgtacca gttgcaataa tatctcccgg atgtagtgca
      241 acatattttg aaatttcttc tattaattca tcaattttaa gaatcatttc gccagtgtta
      301 ccatcttgtc taatttcatt gttaactttt gtaacaatat ttacattttc aggtaatggt
      361 agttcgtctt tagtaacgat ataaggaccc attgggcaac cgccagttaa actttttgat
      421 aaaaatgctt gatcttgttc actttgtgct ttgcgatcag tgatatcgtt aataattgta
      481 tagccgtaaa cataatctaa agctaatgct tttggaatct tttcaccaga cttaccaata
      541 acaataccta attcaccttc ataatctaat tgatcagtaa tatctttatg atttggaatt
      601 gttgcattat ctcctgttaa agatgacgct gcttttgtaa atacatataa tttttctact
      661 tcatgattta attcgttcgc atgatctttg taatttctac caaaagcaat cacattattc
      721 ggaggtgtta ctggtggtaa gaattcaatg tcattaaatg aaattttata gtcttcagct
      781 ttgccgctat cttctgctgc tacaactgct ttacgtactt gttcttgaaa atctaaagta
      841 tgattttgtt gtaaaccagc taacaatgtt ttaggatgga aatctccttc tgcaaagtca
      901 gcaaatactt gtgttaaatc ccatacagca tcttcgcgtt ttactttaac gccatatgaa
      961 gttttgtcat tatacttgaa tgataagaat ttcattcatt ctcaactcct catctttatc
     1021 ttaattcaca ttataacttt tttcgttatc aaataacaaa taaataagta agacaatttt
     1081 gaaaatgagt tatgttcatt ctgctacaag ggctttgcac ttaatcgaaa ttatttttta
     1141 ttcttttgaa aatcaaaata ctacagttgc aatgtaccaa atttgaagaa gtataaataa
     1201 cctttaactt ctttattaag tatcgtttga agcgtatttt gataatattt catctgtatc
     1261 ttatatttat tttttaattg tgtaccaatt tcttcatctg tcatcccacg gcgacgatta
     1321 aatgcatcgg ttttatagtc tacaaaataa tgcacaccat ctttaacaaa gattaagtca
     1381 atcatacctt gaataattga gacgtcttcg tctccttgtg gcaattggtc aactaatgct
     1441 tggttaacta caaacggtaa ttcacgataa acttgctctg cttcagcaat aatcgaatat
     1501 aactcactat tgataaatgt cattatttca tccatacgga tatctttttt cgcatctgct
     1561 tcgataatat gtttatcgat taatccatcg atatactgat gtaactcaac ttcagatatg
     1621 cgttcttttt tgaatggtaa atgttgcatc actgtatgca ttaacgtacc aatttcattc
     1681 gcttttcgtt taccttgttc acttagaaat ttaggtcgtt catacgttga aaaaccgata
     1741 cgatattgtc ttactcgttc gtaacttgtg ccactttctt ctgtttcata ttgccttttc
     1801 aattcagaaa cagattgttt tgatggcttt ttagtatcat ttacatatgg atattgataa
     1861 tcaagttggt gtttaatttg tgctttaaca tcctcattac cattttgcat agtttctaat
     1921 tgattaaccg aacgatattc attattatct aaaatggttt ctgtagacac atcttcaaag
     1981 tatataattg aaatatttac attcggacga ctactatctt caacttgtgc tatatctttt
     2041 tcaaatttta aatcatctgg aattgacgca gattgatgtt tagataaaat actataaata
     2101 agatggaacg gatttggtga agttaatcgt tcattgacag caatgtgctc accagaaata
     2161 gacaattgct ctagttctag taatgattta tcatttttca ctctaccaat taaataaagt
     2221 tgttctttcg ctcttgttaa tgctacatag actaatcgca tttcttctga cacaagttct
     2281 ttttcggcaa cagctctata tgcaaccgaa gctaaagatg gaaatgccat ttctttatcc
     2341 acatcaaaat aatccattcc gagaccaaat tgctgattta aaataactgg ttgtttcaaa
     2401 tcacgtttat taaaatcttt tgacaatcca gaataaatga caaatggaaa ctctagacct
     2461 ttactactat gaattgtcat cattctaacg acattatcgt ttggaccaac tacattttcc
     2521 tcaccaaaat ctttgcctct ttcaatcaat tcatcgataa aacgaataaa ttgatataaa
     2581 cctctaaaac ttgaattctc aaactcgata gctttattaa ataaaccata aagatttgca
     2641 cgtcgtccac gtccaccaat aagtccacta aagtattgaa taacataatg atcattataa
     2701 aatttatcaa ttaactgata caccggatga tctttactat attgttggta actttgaata
     2761 tctgataaaa acatttttaa tttatcaact aaaattgcat ctgctgcttc gtcattaatg
     2821 taatttacaa tcgattgata gaagtagtca tcatttggac tcaatattct aatttgagct
     2881 aattcgtctt ctttgaactg atatataacg gagcgcatta acccaactaa ataaatatct
     2941 tgtaatggat tatctatcgc tcttaaaaat gataatacta agcggacttc tgtttgttca
     3001 aagtaacctt cacgactatt cacatggaat ggaatatctt catttttaaa ggcttgttgt
     3061 aaattgcgag cttgtccaaa gctgcgttct agaataacga tatccttgta tgtcgcactt
     3121 ctatagcttc ctgttttcat atcataaact ttttgatgtt ctaagatatc tttaacttgt
     3181 tctactataa aatgcgcttc ttgttcacta ccagttaaat cactatgttc ttgatccgct
     3241 tcaacaagga cttttaagtt tactggatgg tccgattcat catatggtgc accataatac
     3301 aactgtgccg cttcatcata ttttacttca ccgacttgtt catccatcat atgtttgaat
     3361 atatagttag tcgttgacag tacttctttt cgagaacgga agttttgcga caaatcaatt
     3421 cgacgtccag tgccatctcc atctatagta aagcgttgat acttttcaat aaataaactt
     3481 ggatcagctt gtctaaattt ataaatggat tgcttaacat ctccaaccat aaataaatta
     3541 ccattatgtt catcacccgt tttgatgcaa gatagtattt tctcttgaac tcggttcgta
     3601 tcttgatact catcgaccaa tatttcttgg aagtgttgac ggtatgattc ggcaatttct
     3661 gaaggcgaac catcctcatt tgttaaaatt tgtaatgcaa aatgttcata atcagaaaaa
     3721 tccaaaatat ttttgctacg cttttttcga ttgaattctg acataacatc tttcacaata
     3781 cgcgcaaggt actttactcg tggcgccaat tgttgcatat cagctttcaa atcatcagct
     3841 tctcttgaaa agtaatcact cttcacttta tcaattaatg atttatattt tttatactga
     3901 agtttagcat cttctaaggc atcgaccatc atttcatttg cttctttaat tttcgctgtt
     3961 acattaggca aacgcgcgcc aaattcgtga ccagttaaat aaggtatatc aataaagcca
     4021 ccttctaaaa cacgccccat taaacgtcgt tcatcttcta taacagctaa atgtttatcg
     4081 acgctatcca tcatactaaa caaatcatac gccttattta aagcatcata agcagctgtg
     4141 ataaatactt tagataagtc tgttagtagt tgaataagtt gcgcttgttg tgcttcttct
     4201 tcgtatggtg tcaccaattg atccaaccaa tttgtaggat ttggatttgc aacgctaaag
     4261 aaatacaatt gtttaataat cattcgaaac tgatcatcac ttctatctga agacaattgc
     4321 tctgttaatt caataaaagc aggatcaagg atatcgtaat gttgttctat gacctcatct
     4381 atcgtttgtt ctaataataa aatattttca gcttcactgc ttgttctaaa gttcgggtca
     4441 atatttaata catcataatg ctgttgaatt aatttcaagc aaaaactatg gagtgtagat
     4501 atttgtgctt gatgaatttt gatgcgttgg tttttcaagt gtgcatttgc aggatcagca
     4561 atcgatgcct cttgaatacg ttggtctaca cgatgcttca tttcacgtgc gcttaagttt
     4621 gtaaacgtta cgactaaaag tcgatcgaca tcaatgccat cacgtaaaat cttttggata
     4681 atacgctcaa ctagtacagc tgttttacct gaacctgccg cggctgcaac aagtacatct
     4741 tgtccagttg cgtaaatact ttgccattgc gcgtcagtcc aaatcacgcc ttgtggtttc
     4801 tctggaattg tcatctattg ctcaccccca aattcatcat taatgttaat attttgaatt
     4861 gcttcaattg gatttattgt ttcatctaca gttcgatatc gcttactatc aatcatgcca
     4921 tctacatgac ataccgattg ataactacaa aaagcacatg gcaatttttg tttgtacttt
     4981 aatggtgcaa cttcagtatg tccatccata atatttgaag ctgtttctat aaaattctct
     5041 ttgttatgtt ggatgaattt ataaatcgtt gcttcatctg ccacttggct gcctcgttta
     5101 ctcaaagagc catctttatt caaaccaact ggtacaatat ctgaagtgaa tttaggttct
     5161 aaacgaatat ccaatgcatc aataacagtt tggtctgcat taactaaacc actcaactta
     5221 aactttttaa ttaaatcttg ttctagttta tcttcatcaa tatcagacca tgatttaaat
     5281 ttaattctag gttcatgtac atggaagtat aataatccac ctggtttcac aatatctgtt
     5341 aatccaaggc gttgtttatt ttgtaaaacg atatccatgt atgtcatcat ttgcatttgc
     5401 ataccataat atactttcgt taaatcaagt gtcgcactac cttcagagga tttatagtca
     5461 atgatattaa caaaacttgt atcattcttt gtatacgtat cgatacggtc aatttgccct
     5521 ctaatattaa ttggaatacc ttgagttgtc gttaatgttt gtgcaattaa ttcgtcattt
     5581 gttcttggtt tccttctaaa acttgtctca aaatgttttg gcataaactt tgaatacgtg
     5641 ccttgatatt ttaatgcgct tagtgttgtt tctacaatag cgccaatgcg tcttgataaa
     5701 taacgatagt aagctgaaga atttaataaa ttaaactgaa ctttaggtaa aatttcttcc
     5761 aatgcttcat tcgttaattg tcttattttt ttcaggtcta attgtttaaa atcgccatta
     5821 atacgttcag atatatattt taaaacagaa tggaaaatat cacctaaatc aaagttttga
     5881 agttcatact tcgttcgctc atttagtttc agaccatgtg acgcatagtg tttaaatggg
     5941 cattgttgat aaccttcaaa acgggataca ctggcattga tttccttacc atataaatct
     6001 ttagacaacg tttcacctaa ttttacagtt tcattgtcaa acgttaatgc tgacattaaa
     6061 taatctaaac cttgatttaa atgatcgcta tctctaatta cttgataagc atctaaccaa
     6121 ctatcagcca caatttcatc atataaccaa gcacgcaatg cttcaaataa tgtaattttg
     6181 gtttgcttag catgttgcat tagtgacaat gggttaactt catggtattg aggaatgtta
     6241 gtaatttcca attggttgaa caatgattga atttgattta aaaatgggct gatctcctta
     6301 tcatcaccac ttgatcccat tagtctgtaa gaaaatgtaa catcttgtcg cgctcttgtc
     6361 atagcaatat agcaaacaaa tgcttcatcc atctgtaaaa tatctgatgt tggacttaac
     6421 tcgacattag cctgctgttc aaagtatttc ttttcttcat ctgtaatcaa gcttgacgca
     6481 gttactggtt gtggcatcgt accatcattc atacctacta agaaaacatg ttgcttatta
     6541 tcgactttag ccaaatccat cgtaccaata cttacttggt ccaatgtttg cggaatcata
     6601 acaaattcta attgttctaa accaatatca aatacttcta agaaacgttc catcgacatt
     6661 ggttcatctc caaatactag aactaagtca tcaaggattt gaattaagcc attccatatt
     6721 tgatcaattt cctccgcctt ttcatgatta ccatttaaat caagttcatc tcgctctgtc
     6781 atcaattgat ttggcagttc gaaatattcc atactttcat aaaaagcagt tgcaaaatct
     6841 tttacagttt ccgcttgtga catttgcttt tcaaaatgta aaattttatc aatgacatct
     6901 ttctttaact taacgacttg ttcaaatgtg ttacgttcat cttcggtcag tttatgcgct
     6961 ttacgcccca ttttgctaaa atgttcgaca ttaaatagct catcatctaa ccaacgttta
     7021 ccgtatatac cacgttcaag tacaaaattt tcaagtaaat caactaagta tgcacttttt
     7081 agatatgatg ccgttaacac atcagtcttc aataagcgta gcattggatt cacttgccaa
     7141 ttagattgaa taacttcaat caatgaacga atcatttcca tgaccggatg atgtgtcatc
     7201 gaacgctttg tatcaatatt ataaggaata ttataaagcg gtaatatgga atcaaataaa
     7261 taagcataag attcatcacg atataaaata gcaatatctt gatatcgtaa ttgcttatca
     7321 cgaatatcaa cgatgatacg tcgcgcaatt tcatttattt cctctctcat agtcgcagat
     7381 tctaaaatat tgatatgacc ttgacatgcc actcgattga tttgaagtac atcaaattct
     7441 tgttcaagat gctttaaatc ttgattattg aagcgatata attggttgaa atattgacgt
     7501 tcaatagaaa tattgagttc atttgctatt tcttcaatat gtcgtaacac ttccgatggt
     7561 tttctaaata aactaaattg atcgtggtta ccatctgtcg tcaatataat tgtgacactc
     7621 ttcgcatatt taattaatcc tttgattatt aaatactcaa tcgttgaaaa gttgtgaaaa
     7681 ccatcaatat atatatcagc acgttttagc cactctgatt tcggcataca atcaataaaa
     7741 tattgtaatg aatcctcacc agtaataaac tcgttttgaa tgcgttgttc gaactcacgg
     7801 tatattaaag caatatcctc taacttattt ttagttcgag tttgcatatt tttatcagca
     7861 ataaagtgtt ctaaatgttc aggcgttact gcatattttt taaaatcttg aatttgttct
     7921 gttaattttt cactaaaccc ataatatttt gcttgtgatt gataaagttt taaatacttt
     7981 tgttgttctt gaacaatgtt ataaatcatc atttccgttg cagctttaga taacttttgt
     8041 tcgctataac taccaacttc ttggaaaata cgatgactta atcgttcaaa atgcaacact
     8101 tctgttctta aactaccatt taattccgga tcattgacaa aggcttgttc taattgaaat
     8161 gtactttgag ttggcgcaat taaaatgatt ggatctccaa gcggatctgc tttcattttt
     8221 tgttttattt cggtcaacat tttcgtagac ttacctgttc ccgctctacc taaataagca
     8281 tgtaatgtca tgacaaaccc tcacttcctt tgacacatat tttaacacaa agtgattgtg
     8341 attgatttat tataagttca tttcgaactt agcttttatt gtggtactaa caaaataaaa
     8401 catcgtttat cagtattaaa accgacaaac gatgttgtat ttgtttcata ttaattttta
     8461 gtattttcag gattgaaatt atgtttaaat tcactaaatg gccagaatct aaatgaaact
     8521 ttaccaacaa tttggtcttc atcaatgagg ccaaacgcac ggctatcttt acttacttca
     8581 cgattatctc caagaactaa atatttacct tttggaatga catttgattt aggattcgca
     8641 ttcggtaaat ctttaacttg gaaagtccca gtaatgtaat caccttgttt atgttttaaa
     8701 ttatagttta aatatggttc atcttgtttt ttaccattga catataatgt atcattttta
     8761 tattctactt tatcaccagg aacaccgatg acacgtttaa catagtcatc atttttgttt
     8821 gcatggaaga caactacatt acctttttcc aaaccacctg ttttatatcc aataatgttt
     8881 acagctactc gctcgccatc tttcaaagtt ggatccattg attcaccttt aattgtatat
     8941 ggtgtaacaa taaatttacc tactataaat aaaatgacaa aagcgactgc aattgaaata
     9001 atccattcca ataattcttt tttcaatttt gacacctctt tttaagattt gaactgaatc
     9061 gtccattttg aaaaaggata atatctcaaa cttatattac caataatatc ctttttatca
     9121 attaaaccaa attgtctaga atcatgctga ttgttatcat gatcatttag cacaacaaaa
     9181 ttgttaggcg gtataatatc tccatctaat tctttaaaat tgcgcaaact aaaatcttta
     9241 atttttctgt tcttggcata agatgcgtca accggtcggt catcacggta taattgtccc
     9301 tgacgaaacg ccattgattg accaggtttg gcaataattc gactagtata tatctcgtta
     9361 ccacgcctat atgtaatgat atcaccatta ttcaattgat taaatgtaac tttaatttta
     9421 tttacaataa cacgatcccc tttgttaagg gttggtgaca tatcattatt cggaatgaca
     9481 tgaccaacta ttacaaaagt ttgtacgaac agtacaatga taatagcaag tatcaatgaa
     9541 atcaaatatt ttacaacttt tttcacgatg tcactccttt ttcgatccca taaaatgctg
     9601 ttctaacttt tttccaacaa tccaaaatac aaccaacaca actaacatta atatccctct
     9661 taaaggatgc gttaaaattg tagtaatttc cttacctaaa taacctaaaa taattgttga
     9721 aactaacttt gatgatgcca aaacaatgaa ataatattta ggtctaatat gagatagact
     9781 cgctacaaaa tttattaatg tatttggcgt aaaaggaaaa caaagtaaaa taaacaatgg
     9841 gattaatcct tggcgatcaa taaaactaat caagcgttga acagcagtac gttgtttaat
     9901 tcgctgcatc ctctcagtgt tcaccaatcg tttacagatc aaatagactg taaatgttcc
     9961 agaaattaat ccaagccaac taatcaatat acctaaaata ggtccataag cttgaatgtt
    10021 aattaaaata tagagtgcta aaggaaatac tggaattatc gctctaatat ataacaatat
    10081 aaatccaggt aaataaccaa actgtcgaaa tatctcaaac cattcttcta cttgatgaaa
    10141 cgacaaatca tcaatccctt tctttggttg aagataatta ttcttacatt ataaagttaa
    10201 ttactaagta aagaaacatt aacatgtaat catgctaata aatcagtctc tagtagtata
    10261 acaatattat tgtatgacag atgttatttt ttctttaatt ttctcttaac atcttgtcac
    10321 attagctagt aacctagttc ttgcatataa ataaaaacga ggttgtcatt acatttttac
    10381 atttgtaatg acaacctcga aaatatgctt taaatagtta acgtctagtt ctatcttttc
    10441 aaacttcact aatcattgaa gtaatgtatt ttataaacgc tcttctaatt cttttttcaa
    10501 gtcttcaaaa ccaggtttac ctaataatgc gaacatgttt tgtttatatg cttctacacc
    10561 aggttggtta aatggattta cacctaattg gtatccactc attgcacaag caagttcgaa
    10621 gaagtatacg acataaccga aagtttcttc atctaattgt ggaatgttca ctaccatgtt
    10681 aggaacacca ccatcagtat gcgctaataa tgtaccttcg aatgcttttg tgttaacttc
    10741 gtcgattgtt ttaccagcca aataatttaa tccgtctaga tcatcactat ctttttcaat
    10801 agtaatatca tatttaggat gatttacttt taccactgtt tcgaataaga aacgacggcc
    10861 ttcttgtaca tattgaccta aagaatgtaa atcagttgtg tagttggcac ttgaaggata
    10921 gatacctttg aagtctttac cttctgattc accaaataat tgtttccacc attcattaaa
    10981 gtattgcata gatggttcat agttaatcaa catttctgtt gtataacctt ttgcatataa
    11041 aatgtttcga attgtcgcat attggtatgc aatgttttct tctaatttat ctgaagataa
    11101 ttcttcacgt gcttttgcag caccaatcat catagcttcg atgttaattc cagcagttgc
    11161 aattggtaat aatcctactg ctgttaaaac agaatatctt ccacctacat catcaggtac
    11221 gataaacgtt tcataacctt cgtttgtagc caactgtttt aaagcacctt tttctttatc
    11281 cgttgttgca aatatacgtt tttgtgcttc ttctttaccg tatctttctt caactaattg
    11341 tttgaacaat ctaaatgcaa ctgctggttc tgtagttgta ccagatttag aaataacgtt
    11401 tacagagaaa tctttgtctg ctaaataatc aactaactct ttcgtatatg ttgatgataa
    11461 gtgattacca acaaatacaa tttcagggta ttcattgctg tttctaaatg atgacgttaa
    11521 catttcgatt gctgcacgtg cacctaagta agaaccacca ataccgatga ctactaaaac
    11581 atcagaattt tctttaatgc gttttgatgc ttcaacaatt cttgaaaatt cttctttgtc
    11641 gtaatcaact ggtaaatcaa cccagcctaa gaagtcacta cctgcaccag taccttcatg
    11701 aattgttttg tgaattgatt taacaatttc ttgttgttgt tttaattcgt gttcaccgaa
    11761 aaattctaac gttttactaa aatctaattg aatatgagtc atctataaaa gcctcctgtt
    11821 tcatttaata tcttaatttt actaagttta cgtttgcgat tcaattcaca agtttgtact
    11881 taatcaataa aattgatatt tatgtgacgt ttcacaccta tttttcaaca aacatttcaa
    11941 actggcgtca aacactcaaa cattttttat acaatttgca tgttttttaa aatactttta
    12001 aacattgata aataagcatt tatacttaca actctaacta tctctatcgt attttatgaa
    12061 taaatatgta aaataatggt tttattttca ctaaaagttt gctatcatgt gttcatacga
    12121 aatattcaga taactacaat gaggtgaatt ttatgaaaga gaaaattgtt ttagcatatt
    12181 caggaggact agatacaagt gtggccgttc aatggcttat cgacaaagga tacgacgttg
    12241 tagcttgttg cctagatgtt ggtgaaggta aagatttaga catcgtttat aaaaaagctt
    12301 tagatatggg agcagttgaa tgtcatatta ttgatgcaac aaaagaattt agtgatgagt
    12361 atgtaagtta tgcaatcaaa ggaaatttaa tgtatgaaaa tgcatatcca ttagtttcag
    12421 ctttatcaag accattaatc gctaaaaaat tagtagagat tgctgagaaa acaaattcag
    12481 taggtattgc acatggttgt actggtaaag ggaatgacca agtacgtttc gaagttgcca
    12541 ttaaagcatt aaacccatca ttgaaagcat tcgcacctgt acgtgagtgg gcatggagtc
    12601 gtgaagaaga aatcgattat gcaattaaac ataatatccc tgtatcaatc aaccatgatt
    12661 caccttattc tatcgatcaa aatctatggg gcagagcgaa tgaatgtggt attttagaag
    12721 atccttatgc tgcgccacca gaggatgcgt tcgatctaac aaatgcttta gaagaaacac
    12781 cagatgctgc tgatgaaatc attttaacgt ttgataaagg catcccagtt caaattgatg
    12841 gcaaaacata tgaattagac gatttaattt taacgttgaa tgcattagct ggtaagcatg
    12901 gtatcggaag aattgaccat gtagaaaata gacttgtagg tatcaaatca agagaaattt
    12961 atgaggcacc tgctgcagaa gttattttaa aagcgcataa agcattagaa acgattacgt
    13021 taacgaaaga tgtcgcacac tttaaaccaa tcatcgagaa gcaatttgct gaacaactat
    13081 acaatggact ttggttctca cctttaactg atagcttaaa attatttatt gatagtactc
    13141 agcaatacgt aagtggtgat gtcagaatta aattattcaa aggtaatgcc atcgtaaatg
    13201 gtagaaaatc accttacaca ttatatgatg aaaaattagc aacttataca aaagaagatg
    13261 catttaatca agacgctgct gtcggcttta tcgatatcta tggtttacct actcaagtaa
    13321 attcgatgct acatggaggc tatagcaatg agcaataaag cttggggcgg tagatttgaa
    13381 gtacaacctg aagagtgggt tgacgacttt aacgcatcca ttacttttga tcaaacgctc
    13441 ataaatcaag atatcgaagg cagcattgca catgcaacta tgcttgcgaa tcaaggcatt
    13501 attagtcaac aagacagcga acaaattata caaggactaa aatctattca acatgattat
    13561 catcaagatc aaattcaatt tagtgcatca ttagaagata ttcatttaaa tattgaacat
    13621 gaattaatta aacgtattgg tgatgctggt ggtaagttgc atactggacg cagtagaaac
    13681 gatcaagttg caacagacat gcacttgtac actaagaaac aagtgcaaga tatcatcgca
    13741 ttaattaagt cgttacaaag tgtaattgta gacatcgctt ccaataatgt tgatacaatt
    13801 atgcctggtt atactcattt acagcgtgca cagccaattt catttgcaca tcatattatg
    13861 acttattttt ggatgttaca acgagaccaa caacgatttg aagatagttt aaaacgaatc
    13921 gatattaatc ctttaggtgc agcagcctta agtggtacca cataccctat cgatagacac
    13981 gagacaacag cattgttgaa ctttggcagt ctctatgaga atagcctaga tgctgttagt
    14041 gacagagact atattattga aacattgcat aatatttctt taacgatggt tcacttatca
    14101 cgctttgcag aggaaattat tttctggtcc acagacgaag ctaaattcat tacattatca
    14161 gatgcatttt caactggctc atctattatg ccacaaaaga aaaatcctga tatggcagaa
    14221 ttaattagag gtaaagttgg tcgaacgact ggtcatttaa tgagcatgct tatgacttta
    14281 aaaggattac ctctagcata taacaaagat atgcaggaag ataaagaagg tttattcgat
    14341 gctgtccata caattaaagg ttctttacgt attttcgaag gtatgattca aacgatgaca
    14401 attaataaag aacgactcaa tcaaactgtt aaagaagatt tttcaaatgc aacggaacta
    14461 gcagattatt tagtaactaa aaatattcca tttagaactg cacatgaaat tgtaggaaaa
    14521 atcgtcttag aatgtataca acaaggtcat tatttattag atgttccttt agcaacatat
    14581 caacaacatc attctagtat tgatgccgat atttacgatt atttgcagcc tgaaaattgt
    14641 ttaaaacgac gtcaaagtta cggttcaaca ggtcaatcat cggtcaaaca acaacttgat
    14701 gttgctaaac aattactatc acaataaata cgttaatcta cctacccaca atgtctatta
    14761 aaattacatt gtgggtattt taatgctctc ttcgtcttgt tgaacatcac atttttaaga
    14821 ttcctaaaat gtttgataat tcttttaaat ttatattaca aaaatgttat aaattgtaaa
    14881 agaaatgtgt aaagcgtttt cacaagcagg tttttgtagt attttaaaat tgttagacta
    14941 caaataaaga gatgaaagga taaagactat gactaactct tcgaaaagct tcactaaatt
    15001 tatggctgct tctgctgttt ttactatggg atttttatca gtacctactg ctggcgctga
    15061 acaaacaaat caaattgcaa ataaacctca ggctattcaa tggcatacaa atttaacgaa
    15121 tgagcgattc actactatcg cacatcgtgg cgcaagtggc tatgcacccg agcatacgtt
    15181 tcaagcatat gataagagtc ataatgagtt aaaagcatct tatatcgaaa ttgatttaca
    15241 acgtaccaaa gatggccatt tagttgctat gcatgatgaa actgttaacc gtacaacaaa
    15301 tggacacggt aaagttgagg attataccct tgatgaatta aaacagttag atgcaggaag
    15361 ttggtttaat aaaaaatatc caaaatacgc aagagcaagt tataaaaatg ctaaagtacc
    15421 cactttagat gaaattttag aacgttatgg cccgaatgca aactattata ttgaaacaaa
    15481 gtcacctgat gtatacccag gaatggaaga acaattatta gcttcattga aaaagcatca
    15541 ccttttaaat aacaataaat taaaaaatgg acatgtaatg attcaatcat tttctgacga
    15601 aagtttaaag aaaattcatc gtcaaaataa gcatgtgcca ttagtaaaat tagttgataa
    15661 aggtgaacta caacaattta acgaccaacg cttaaaagag atacgctctt atgcgattgg
    15721 attaggtcct gattatacag atttaactga acaaaatacc catcatttaa aagacttagg
    15781 atttatagta catccttata cagtgaatga aaaagctgat atgttacgat taaataaata
    15841 tggcgttgat ggtgtcttta caaatttcgc tgataaatat aaagaagtca ttaagtagta
    15901 atgttaaact agaaaacata aatacaaaaa tatagctatt actataaaaa acagcagtaa
    15961 gatatttcca aattgaaatt atcctactgc tgtctttttg ggagtgggac agaaatgata
    16021 ttttcgcaaa atttatttcg tcgtcccacc ccaacttgca ttgtctgtag aaattggctc
    16081 cccaatttct ctgtgttggg gcccatcccc aacttgcatt gcctgtagaa tttcttttcg
    16141 aaattctctg tgttggggcc ccgactattt ttgaaaagag cgtgttacac gggcattgtt
    16201 ttacagtcaa ctactgctaa aataaaatta acgagcttag ggctttgttt tctgtcccaa
    16261 gctcgttaaa tcacatatga taattaatta tgcccaacca cgatatctag ctgcttctgc
    16321 tgtacgttta atacctatga tatatgctgc aagtctcata tctatttttc ggttttgaga
    16381 caattcgtaa atcgtatcaa atgccgcttc taatttttca cgtagctttt cattaacttc
    16441 ttcttcagac caataataac cttgattatt ttgtacccat tcgaagtaag aaaccgttac
    16501 accaccagca cttgctaata cgtctggaac taataatata ccacgttcag ttaaaatacg
    16561 tgttgcttct ggtgttgtag gtccattagc agcttcaaca acgatactag ctttaatatc
    16621 atgtgcattg tcttctgtaa tttggtttga aatagccgct ggtactaaaa tgtcacaatc
    16681 taattcaaac aattctttat ttgagattgt ttcttcaaat aaatttgtta ccgtaccaaa
    16741 actatcacga cggtctaata aataatctat atctaagcca tttggatcgt gtaatgcacc
    16801 gtaagcatca gagataccta caatttttgc acctaaatca tataagaatt tagctaagaa
    16861 acttccggca ttaccgaaac cttgaataac aaccttggca ccttcaattt gcatattacg
    16921 acgttttgca gcttgttcaa ttgcaataac tacacctagt gcagttgatc tgtcgcgtcc
    16981 atgagaacca cccaatacaa ttggtttacc tgtgatgaaa cctggtgaat taaatttatc
    17041 taatgcacta tattcatcca tcatccaagc cataatttgt gagtttgtaa atacatctgg
    17101 tgctggaata tctttgttcg gacctacgaa ttgtgaaatt gctcttacat atccgcgtga
    17161 taaacgttca acttcatgaa tgctcatttg acgtggatca caaacgatac cacccttacc
    17221 accaccgtat ggtaagttta caatgccaca tttcaaagtc atccacattg ataatgcttt
    17281 tacttcttct tcatcaacat ctgggtggaa acgcacgccc ccttttgttg gtccaacagc
    17341 atcattatgt tgcgcacggt aacctgtgaa tgttttaact gtgccatcat ccattcgtac
    17401 agggatacgc acttgtaaca ttcttaaagg ttctttaatt aaatcgtaca ttccttcgtc
    17461 aaatcccaat ttatgcaatg cttctttaat aattccttga gtagaagtta ctaaattatt
    17521 gttctcagtc atgatccttt tcgcctcttc tttacctaat gatttcgctt tcaaacatat
    17581 tgtaacataa cgtattcctt tttaaagccc ttacaaactg attgttacaa ctttttgaca
    17641 ttattgaaat acatgtctta ttttttcaag tgcaaggtcc aattcttctt tagtaataat
    17701 taatggtggt gcaaaacgaa tgacagtatc atgcgtttct ttacataata agccttcttc
    17761 tttcaaagct tcacaatatg gtctagcact ttcattaagt tccacaccta taaacaaacc
    17821 acgtccacgg acttctttaa ttgatggatg atcaatttgc tttaattgtt ctttaaaata
    17881 atctcctaat tctaaagagc gccctggtaa atcctcatca acgataacat ctaatgcagc
    17941 aattgatgca gcacaagcaa gtggattacc accaaatgtt gaaccatgtg agccaggtgt
    18001 aaagacatct aatacttctt tatctgctaa tacaacagaa attgggaaga caccaccacc
    18061 tagtgcttta cctaaaatat agacatcagg ttttacatta tcccaatccg tagcaaataa
    18121 tttacccgaa cgacctaatc ctgcttggat ttcgtcagca ataaataaga cattatgttc
    18181 atcacataat tctctaattg ctttcaaata tccttctggc ggtatattta tacccgcttc
    18241 accttgaatt ggttctacta aaactgctgc agtattttca ttaattgcag ctttcaatgc
    18301 atctacatct ccaaaatcaa cttttctaaa tccatctaat aacggaccat aaccacgttg
    18361 gtattctgct tctgaagata atgaaactgg cgccattgtt cgaccatgga agttaccatt
    18421 aaatgcaatg atttctgctt tatttggctc aattccttta acatcgtatg cccagcgtcg
    18481 tgctgctttc aaagctgttt ctactgcttc agcacctgta ttcattggta aagctttatc
    18541 tttacctgcc agtttacaaa ttttttcgta ccattcacct aagttatcac tatgaaaagc
    18601 acgtgaaact agagtcactt tatcagcttg atcttttaat gcttgaataa ttttcggatg
    18661 tctatgacct tggttaacag cggaatatgc agataacata tccatatatt tattgccttc
    18721 aggatcttta acccataccc cttcagcttc tgaaatgaca attggcaatg gtaaataatt
    18781 atgtgctccg taatgatttg ttaactcaat aattttttca gatttagtca tcatatctcc
    18841 ccttttcatc atttataact attatacatg aaacattatc caaataatta cattagtttt
    18901 caaagcagat acttttccac caaaaaagat gaaataatca ctaagtttca ttaaatttgt
    18961 ctattttgaa aacccttaca tttataatga cataattact taaatgatta caagcaaaag
    19021 aattgataat tttacactta atcaaaagta tattttacta agaatatttt tatttataaa
    19081 tattgaaaac cactaacaaa ttgcatacac aatatcatta gtggtaacag ttaaacactt
    19141 atttatcttt acgtggtaat ggatagaatc cttcattaaa ttgatgccat aaattaggag
    19201 ttaagtgatg attatcttct ctctctggat caaagtaatt aataatttca tcttcccgtc
    19261 catctttaat tttttcaaca aattgataat ccagtagtag ctctctacca atggctacta
    19321 agtcaacacc aacattttca actgcatcta aagcttcgtc agctgtgaaa attgaaccaa
    19381 taccgataag tggcatacga ccatttatcc atttgtgaat taaaggcagt ctttcttgtc
    19441 cagcgtattt accttcacgt gtcgttgcat gcgtatccat catcgaaaca tgaatatagt
    19501 cgattggcat atggctaatt ttattaacga gttcctctgt aatttccatt gtgattcctg
    19561 gagactccgc ttcctctgga gataatctgt atccaactat aaagtcttta ttgccatacg
    19621 cttctttcgc tttaagcacc tcttcaatca cagcgaccgg gaatttatat tgatttgccc
    19681 aaacatcatt tcttctatta tagtatggtg atacaaattg atgaattaag taatgattcg
    19741 cgccatgtat ttcaacacca tcaaatcctg cttcaattgc acgtcgcgtt gcttcaccaa
    19801 aatccttgat tgcttgttca atctcttcat tcgtcatttc tctagcacta tgttcttgtt
    19861 tctgaccgaa actttttaaa gaaattggac ttggtgctac gacgtctcca ccaggtgtta
    19921 attcaggcaa tgcttgtgca ccgccatgat gtatttgtac gagtgctttg gcaccgtttt
    19981 tcttcattgc tgtagctaat cgttttagtc cttcaatatc actgtcatgc gcgattgatg
    20041 gctgtcctgg aaatgctttt ccgacatcac tcacattact cgcagcatta attgtaatac
    20101 caacatcttg tgaacgcttt tcaatataag gaagttctac atctgaaata gtaccatcat
    20161 catttgaaga aatatgtgtt aaaggggcta acacaaatcg atttctcaac tctactccat
    20221 ttggtaattc tactttatca aacaatggtt cgtatttact tttcattact taattcctcc
    20281 ttaaaattat tgagttgact ataacatgta attttttcat tctccaatta tctgtttata
    20341 tagttgcaat tcgtttaata ctttaagttg tagttctcat ttctaaaata aattaaaaaa
    20401 acaaatccac atacaatatt gcaaatcaat gattctatct atcaaagata atggatatgt
    20461 caccttaaac catttgtacg tgtaaataga atcaatcata ttacgttatc gtagacaaaa
    20521 agagattagt atctaataaa atttaataga tactaatctc ttttatagtc taagaaactt
    20581 tcatttcaga ccctttgtat gattttcggt acgatttatc tgttccttta gtcgtttcga
    20641 attgctcgct ttgactgttt tatccagatt ggtaagcgtt ctttaagtgt ttgaaaccca
    20701 tatttttctg tttctctgat ttcatctaat actgattgtt tttccttctt acgctcataa
    20761 tttttgaagt aatcattatc ctttaatgat agattaagct ttccttcatc atctatagac
    20821 aaaattttag ctttaacaat ttggccttct gatagaaatt tcttcaaatt atgaacgtag
    20881 tcatccataa tttctgatat atgaatcagt ccttcagtat gattaggggt ctcaacaaac
    20941 gcaccgtatg gttgaatacc agttacacgc accttgatat gttggccaat tttgtagtta
    21001 ttcaacttaa taacccctta tttcgtttac ttaacactat tatcatagca caaatcgaaa
    21061 tggtggacta attaaaacat caaatcatac gaatattatt ttataaatga ataagaattc
    21121 tacaaacttg gatttacaaa taaaattagc aacaaactcc tatctaaatc aatatgatat
    21181 ccagctactt tgttttcaat ttattaacta tttgaatagc aattctcttt aaaaacataa
    21241 aaagcaacct caactattat ctttagaata cacatctatt taaaaactgt gctttcttga
    21301 taaatatatt gagattgctc atcatgttag tttatttgtt tcgttggaat atatactaat
    21361 ccattcataa attagcttta tccgagttta aaatgttggt agttaattat gtttagctat
    21421 tattcttcaa catcaataga ttcaattaca acatcatgaa gtggtttgtc ttgtggtccc
    21481 acttttgtat ttgcaatatc ttctaaagta gtttcaccat caatgatttg accgaatact
    21541 gtatgttttt gatctaacca tggtgtacca cccttttcgc catatgcatc aacgattggt
    21601 tgaggccagc caccatctgc aagttgactt aacatatttt gaggtacttc tttcatttga
    21661 acaatgaaaa attgtgaacc attagtatta ggtcctgagt tagccattga taatgcgcca
    21721 tataagttaa atgcatttaa tgaaaattca tcttcaaaag caccgccata aatactttcg
    21781 ccacccatac cagtagctgt tggatcgcca ccttgaatca tgaagtcatt aatgacacgg
    21841 tggaatgtta ttccatcata ataaccattt tttgcatgtg tcacaaaatt ttcaactgtt
    21901 tttggtgcaa tatttggaaa taatttgaat gtcatgtcac ctttatttgt gtgcataacc
    21961 actttaattt caccttgttg tacttctttg tttaactgtg gatagttagc catttatatt
    22021 ctccattcat gttatgatac atatatatta acatactttt aagggaggaa aatagcatga
    22081 agctatatcg attttcacac aaaacaggta gctatggtgt cacgattgtt gaagaaaatg
    22141 gagacgatgt ccttgttaaa gttgaacaag ttatcaaaca cccgaaacaa ggtgatttac
    22201 ataaccctac tgaaactgaa ggtgtatttt tccatgaacg caaagcatta agccattttg
    22261 aaaaacgcta tacgaaacgt tcacaattac gcgacttcaa tgttgatgta ttgcgctatg
    22321 aagattcact tcaacaagca attacagatt tagaaactaa acttaaagct cagcaaacaa
    22381 aacatgctga aatgtcttta gctagtttag cacgcttaaa agaagattat tcacttcaat
    22441 acaaagaaaa ttttaaataa aaaatgacgt gtttcttctt atttaaatac ttctgaaaat
    22501 aaagcacaac aaggagtgtg tacgtttttc agaactatat cattcatgta taatgaataa
    22561 cataaatatt ttttaaagga ggacaacata gtgagtttgt tacatattgc ggtgatatta
    22621 ccgttaatat tcgcactcat tattcctatc ctgtatagat tttttaaacg tatacattta
    22681 ggatggttcg tattacctgt ccctatcgtc atatttattt atatgcttac attaattaaa
    22741 acgacgatgt caggtaatac tgtaatgaaa accttaaatt ggatgcctca ttttggtatg
    22801 aactttgatt tatatcttga tggcttaggt ttactcttta gtttgcttat ttcaggaatc
    22861 ggcagtttag tagtattata ttcaatcggc tacttaagta agtcagaaca actagggaat
    22921 ttttattgtt atttattact atttatgggt gcaatgttag gtgtcgtact ttcagataat
    22981 gttattattc tatatttatt ctgggagcta acgtcatttt ctagtttttt acttatttca
    23041 ttctggcgcg aacgacaagc atctatatat ggtgctcaaa aatcgctcat aatcacagta
    23101 tttggtggtc tatcattatt aggtggtatc atcttactcg ctataccaac acaatcattt
    23161 agcattcagt acatgattca acatgcttct gaaattcaaa atagcccatt cttcattttt
    23221 gcgatgattt taatcatgat tggtgctttt acaaaatctg ctcagtttcc attttatatt
    23281 tggctaccag atgcaatgga agcacctaca ccagtcagtg cttatcttca ttcagcaaca
    23341 atggttaaag cagggttata tttaatcgct cgaatgacac caatcttcgc agcatcgcaa
    23401 ggttgggtat ggactgtcac actcgttggt ttaattacat tattctgggc ttcgctaaac
    23461 gcaacaaaac aacaagactt gaaaggtata ctggcatttt caacagtatc ccaattaggt
    23521 atgattatgg cgatgcttgg aatcggtgcc attagttatc actatcaagg cgatgatagc
    23581 aaaatatatg ctgctgcatt tacggctgca atattccatt taatcaacca tgcaacattt
    23641 aaaggtgccc tatttatgat tacaggtgca gtggatcatt ctactggtac acgtgatgtt
    23701 aagaaattag gtggcttact aacaattatg cctatttcat ttacaatcac tgttattaca
    23761 gcattaagta tggctggtgt accaccgttc aatggtttct tatcaaaaga atcattttta
    23821 gaaacaacat ttacagcaag ccaagcaaat ttattcagtg ttgatacatt aggctaccta
    23881 ttcccaatca ttggtatagt gggcagtgtc ttcacatttg tatattcaat taaatttata
    23941 atgcatatat ttttcggaca atataaacct gaacaattac ctaaaaaagc acacgaagta
    24001 tcaatattaa tgcttttatc accagctatt ttagctacat tagtaattgt atttggttta
    24061 ttccctggca ttttaactaa ttcgattatt gaaccagcta catcttcgat taatcatacg
    24121 gtaattgatg atgttgaatt ccatatgttc catggattaa cgcctgcctt tttatcaaca
    24181 ttagtgattt atattttagg tatcttacta attgtgacat ttagttattg ggtgaaattg
    24241 ttgcaacgac aacctggtaa actaacattc aattattggt acaacagaag tgcaaatgtt
    24301 attccaaact actctgaaaa gatgacgaat agctatgtta ccgattattc acgtaataat
    24361 ttagttatca tttttggtgc attaatttta ttaacatttg tcactatatt ctcagtacct
    24421 ttcaacatta attttaaaga cgttagtccg attagaattt tcgaagtttg tatcgtaatt
    24481 ttattattaa gtgctgcctt tttaattctt ttcgctaaat caagattatt tagcatcatt
    24541 atgttaagtg cagttggata tgctgtgtct gtattgttta tattctttaa agcacccgac
    24601 ttagcattga cacagtttgt cgttgaatct atttcaacag cattgttctt attatgcttc
    24661 taccacttac caaatttaaa tcgttataat gaaaagcgct cattccagct tactaatgct
    24721 ttaattgctg gtggtgttgg tttgtccgtc attattatcg gtttaattgc atacggaaat
    24781 agacattttg aatcgatatc taagttttat caagagcacg tttatgattt agctcacggt
    24841 aaaaacatgg taaacgtcat acttgttgat ttccgtggta tggatacttt attcgagtca
    24901 tctgtgcttg gtatcgcagg attagctgta tatacaatga ttaaattacg taaaaagcgt
    24961 caaacgcaag gtaacgaggt gaaaaatcat gaatagacaa caaaatgatc taattttgca
    25021 atttgctgca gttattatct ttttcatggt aatggtcttt ggtttttcac ttttcttagc
    25081 aggacattat acacctggtg gcggtttcgt cggcggatta ttattcgcaa gttcacttgt
    25141 tatcattacg attgcatttg atatagaaac aatgcgtaaa attttcccat tagattttaa
    25201 aatcttgata ggtatcggat tagttttttg tattgcgacg cctatcgcaa gttggttttt
    25261 aggcaaaaat ttctttacgc atgtcacatt tgatataccg ttatttatat tagaacctgt
    25321 tcatatgaca acagcagtat tctttgattt cggtgtttta tgtgcagtag ttggtactgt
    25381 catgacaatt attatttcga ttggagagaa cgaatagtgg aaattattat gatttttgtt
    25441 agtggtattc tcacagcaat tagtgtctat ctcgttttgt ctaaaagtct gatacgaatt
    25501 gttatgggaa ctacactatt aacacatgca gcaaatttat ttttaataac tatgggcgga
    25561 cttaaacatg gtactgttcc aatttatgaa gcgaacgtaa aaagctatgt tgatcctatc
    25621 ccgcaagcac ttattttaac agcaatcgtt atcgcctttg cgacaacagc ctttttctta
    25681 gtattagcat ttagaacata taaagaatta ggcacagata acgttgagag tatgaaagga
    25741 gttccagaag atgattgaaa gtaatatgct tgtgttaacg ctagttattc cagtaattac
    25801 agccatatta cttgtcttta ttggaaagcg tcctattata aagcgttatg tcgcactagg
    25861 cggtacatta ttaaccttgg tcgcggcaat tatcaattta gcaaatgttg ttaaacatgg
    25921 accaatacgc gtagagctag gttcatggaa agcaccatac agtattgtct ttgtattaga
    25981 catattcagt gccctactaa taattactag
//
LOCUS       DAMZLE010000024        25742 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMZLE010000024 DAMZLE010000000
VERSION     DAMZLE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.024"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            60..173
                     /locus_tag="POB92_000893"
     CDS             60..173
                     /locus_tag="POB92_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578410.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            246..1235
                     /gene="ccpA"
                     /locus_tag="POB92_000894"
     CDS             246..1235
                     /gene="ccpA"
                     /locus_tag="POB92_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDI6578411.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1333..2502)
                     /locus_tag="POB92_000895"
     CDS             complement(1333..2502)
                     /locus_tag="POB92_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDI6578412.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENSQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2527..3159)
                     /locus_tag="POB92_000896"
     CDS             complement(2527..3159)
                     /locus_tag="POB92_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6578413.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3328..5034
                     /gene="acsA"
                     /locus_tag="POB92_000897"
     CDS             3328..5034
                     /gene="acsA"
                     /locus_tag="POB92_000897"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDI6578414.1"
                     /translation="MKVEVYKGAQGKHNLKDYEEIYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5474..7141
                     /locus_tag="POB92_000898"
     CDS             5474..7141
                     /locus_tag="POB92_000898"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDI6578415.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(7387..7476)
                     /locus_tag="POB92_000899"
     CDS             complement(7387..7476)
                     /locus_tag="POB92_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578416.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            7475..10162
                     /gene="harA"
                     /locus_tag="POB92_000900"
     CDS             7475..10162
                     /gene="harA"
                     /locus_tag="POB92_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDI6578417.1"
                     /translation="MNKHHPKLRSFYSIRKSILGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKTYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSDASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDVQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPDSDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKGADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HNGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10293..10385
                     /locus_tag="POB92_000901"
     CDS             10293..10385
                     /locus_tag="POB92_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578418.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10365..11270)
                     /locus_tag="POB92_000902"
     CDS             complement(10365..11270)
                     /locus_tag="POB92_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6578419.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11652..12914
                     /locus_tag="POB92_000903"
     CDS             11652..12914
                     /locus_tag="POB92_000903"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI6578420.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(13008..14282)
                     /locus_tag="POB92_000904"
     CDS             complement(13008..14282)
                     /locus_tag="POB92_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI6578421.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14454..15071
                     /locus_tag="POB92_000905"
     CDS             14454..15071
                     /locus_tag="POB92_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDI6578422.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            15199..16665
                     /locus_tag="POB92_000906"
     CDS             15199..16665
                     /locus_tag="POB92_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDI6578423.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSEIELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16772..17902
                     /locus_tag="POB92_000907"
     CDS             16772..17902
                     /locus_tag="POB92_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6578424.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17863..17958)
                     /locus_tag="POB92_000908"
     CDS             complement(17863..17958)
                     /locus_tag="POB92_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578425.1"
                     /translation="MKPLVQSFTSSCAVKQQSNYKLSSTPLSSPK"
     gene            complement(18052..19656)
                     /locus_tag="POB92_000909"
     CDS             complement(18052..19656)
                     /locus_tag="POB92_000909"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6578426.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19643..20803)
                     /locus_tag="POB92_000910"
     CDS             complement(19643..20803)
                     /locus_tag="POB92_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDI6578427.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20917..21363)
                     /locus_tag="POB92_000911"
     CDS             complement(20917..21363)
                     /locus_tag="POB92_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDI6578428.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGLGT
                     NPDELLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21443..22186
                     /locus_tag="POB92_000912"
     CDS             21443..22186
                     /locus_tag="POB92_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6578429.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRISDYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(22402..23004)
                     /gene="rpsD"
                     /locus_tag="POB92_000913"
     CDS             complement(22402..23004)
                     /gene="rpsD"
                     /locus_tag="POB92_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI6578430.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23248..23712)
                     /locus_tag="POB92_000914"
     CDS             complement(23248..23712)
                     /locus_tag="POB92_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDI6578431.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            23849..25543
                     /gene="ezrA"
                     /locus_tag="POB92_000915"
     CDS             23849..25543
                     /gene="ezrA"
                     /locus_tag="POB92_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDI6578432.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(25617..>25742)
                     /locus_tag="POB92_000916"
     CDS             complement(25617..>25742)
                     /locus_tag="POB92_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578433.1"
                     /translation="SLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRIVG"
BASE COUNT         8934 a         3783 c         4673 g         8352 t
ORIGIN      
        1 atttttcaac aaatgcaatt gatatttgaa aaggctttct caaaacatta caattaaaaa
       61 tgaaaaaagt ttatataaaa ttaaaatata tcgttcgtta tcatttagcg tttgttttta
      121 tttcaagctt ttcgctaaat ttttccaaac aaaaatatgt tactgtaaat taaaatatgg
      181 taaactatga aaatgaaatg aaaacatgtt attataatga ataaaacgtt tacaaggagg
      241 aaattatgac agttactata tatgatgtag caagagaagc gcgtgtctct atggccacag
      301 tgtcgcgtgt tgttaatggg aaccaaaatg ttaaagcaga aactaaaaat aaagttaacg
      361 aagtcattaa gcgtttgaat tatcgtccaa atgctgttgc tagaggttta gctagtaaaa
      421 agacaacaac agtaggtgtg atcattccag atatatctaa tatctattat tcacaacttg
      481 ctcgtggact tgaagatatt gcaacaatgt ataaatatca ctcaattatt tcaaattcag
      541 ataacgatcc tgaaaaggaa aaagaaattt ttaataactt attaagtaaa caggttgatg
      601 gtattatttt ccttggtggt acaattactg aagaaatgaa agaattgata aatcaatcat
      661 ctgtacctgt agtagtatca ggaacaaatg gtaaggatgc acatatagca tcagttaata
      721 ttgattttac tgaagctgcg aaagaaatta cgggagaatt aattgaaaaa ggcgctaaat
      781 catttgcttt agtaggtgga gaacattcta aaaaagctca agaagatgtt ttagaaggtt
      841 taactgaagt gttaaataaa aatggccttc aattaggtga tacattgaat tgttctggtg
      901 ctgaaagtta taaagaaggc gtaaaagctt ttgccaaaat gaaaggcaat ttgccagatg
      961 ccattttatg tatcagcgac gaagaagcaa ttggtattat gcatagtgca atggatgctg
     1021 gtattaaagt tccagaggaa ttacaaatta ttagtttcaa taatacacga ttagttgaga
     1081 tggttagacc acaactttct agtgttattc aaccattata tgatatcggt gcagtaggga
     1141 tgcgcttatt aacaaaatat atgaacgatg aaaagataga agaaccaaat gtagttttac
     1201 ctcacagaat tgaataccga ggaactacaa aataaattca caaaattagg cattcatcta
     1261 acgacccaaa ttatatgggt gttggaagaa tgccttttat ttatctttta aaatcgttgc
     1321 agattaggtt acttattgac gagtagattc gtaccaactc gctatatgta aagctaattt
     1381 tttatttttt tcactaattt cttttgtgcg ggggacatag gtataatcat ttaaacgatc
     1441 ttcccatctt ttaggtaata attcagatga ataatgtttc catttattaa tccattctaa
     1501 cggtaaataa ccactttgaa ttggttgatc aattaaactt aagaatacat gactccatgc
     1561 acgtggtacg actctccaaa tattgtagcc tccgccacca aacataatta cctttccatt
     1621 cgtataagaa tcagctaaat attttacaaa atatggaatt tcatataatg aatgtaacgt
     1681 acaatttaga tgagttagtg gatcacgata atgtatatcg acaccattta cgcttagaat
     1741 aatatcaggt ttaaaactct ttacgacagg ctcaactgtt aatttaaaac actccaaaaa
     1801 tgatgcatct tctgtatacg gttcaagtgg gacatttaca gtgtgtccat agccgatatc
     1861 ttcaccgcgc tcagtatagt gaccagagcc tgggaaaaga aattttccgg tttcatggat
     1921 agaataagta gtaacatggt tatcggcata gaaactccat tgtgtaccat ctccatgatg
     1981 tgcatcggta tctatgatta aaacgcgttg attgtattct ttagctaagt attgtgcggt
     2041 aattgcaata tcattgtata tacaaaaacc actagctcga ccaggttgag cgtgatgcaa
     2101 tccaccacct aagtgacaac catttaatac tttgcctgac ataataagat ctgctaaagt
     2161 taaagcgcct ccaacaattg tggcactatg gcggtgcata tgcttaaatt gactattctc
     2221 ttcatcattt aatccatatt tcttagcctc atcttcactg ataatgccat gtgaagcatg
     2281 cttaattgct tcgacgtaat catatttatg aattaacatt aattcgtcat ctgttgcaat
     2341 tctaggttgt actatttgtt ctggagacaa taaatttgca ttcaaaagta gctctgttgt
     2401 taattttaaa cgcatttgat tgaagggatg ttggtcatga aatcgatatt gtaataactt
     2461 atctgaataa acatatgcag tttttgatga atgttgttgc atataatccc tccgatattc
     2521 caaaaattaa aagaaaaacc gattcatata acgaatatca tcaaacgctt gttgctgttc
     2581 taatgtaata tttttgccaa ttcttgccat taaacaatta gctggatgac ttgttatttc
     2641 tggatcatct gtagcgaata tttcaagtcc accagttgcc attaaccgct gcattaattt
     2701 tttatagtca aatacatcta actttgaatt ttttaaatcc caatgccagt aatattcagt
     2761 agttataacg atataatcct cgaattctgg tgtagaaagg ctaagttgta ttagcttttc
     2821 tgcaagatgt aattgcctaa aattgatgct gacttcaatt gcccccaatt cgattaaata
     2881 tggaaggtta cctgtagacc agcgctcaat ttcatcagga tagtggaaag tgacataacc
     2941 cacaatgagt tgatcttggc gagcaacata aattctacct tctggtaatg ttgtaatttc
     3001 taacaaagct tcatactgat cttcagcatc tctaaatgcg gttaaatgcg catcgaaagt
     3061 aagcgctttc aaatcttcgt atgttaaagg accttcaata acaaattgct tgtcatgaat
     3121 gtaataatct tcggattgat acgtctttaa atgattcata ttttcaactc ctcaatcgac
     3181 gtcgtgattg tattaaattc attatataga aaatttacaa taattaatac tagaaaaagg
     3241 ataaaagtaa aaattttgaa taattagaaa tgttatgtat aatattgaga aagaaagcgt
     3301 tttcacataa caaaggggga gtttcaaatg aaagtcgaag tttataaagg agcgcaaggt
     3361 aaacataacc ttaaagatta tgaagaaata tataatactt ttgattggaa agacgtagaa
     3421 caagcatttt cttggagtga aactggaaaa atgaacatgg catatgaatg tatagatcgg
     3481 catgtagatc aaggattagg ggataaaata gcgttaaatt acaaagatga gtacagaaaa
     3541 gaatcgtata cttataaaga tatgcaacgg ttatctaata aagcagcgaa tgttttgtct
     3601 gaacatgcag aagttgacaa aggtgacaga gtatttatat ttatgtcgcg tacacctgaa
     3661 ctatattttg cgttgttagg tgttttaaaa attggtgcaa ttgttgggcc gttatttgaa
     3721 gcatttatgg aaaaggcagt tgcggataga ttagagaaca gtgaagctaa agtgttaatt
     3781 actaataagg cattgttacc tcgagtacct gtagataaat taccgaactt gaaaaaaatt
     3841 gttgtcgtag atgaggatgt agaagacaat tacatagact tcattagttt gatggaaact
     3901 gctagcgatg aatttgacat tgaatggtta aagtcggatg atggtttgat tttacattat
     3961 acatcaggtt ctactgggca acctaaaggt gtattgcatg ttcaacaagc aatgttagtg
     4021 cactatattt ctggaaaata tgtattagat ttacaagaag atgatgttta ttggtgtaca
     4081 gcagatccag gttgggttac aggaacatct tatggtattt ttgcaccatg gttaaatggc
     4141 gctacaaatt gtatagctgg tggtcgcttt tcaccagaac agtggtatag tatgattgaa
     4201 gattttaaag tgacgatttg gtatacggca ccaacagctt taagaatgtt aatgagtgct
     4261 ggtgacgata ttgttgagaa atatgacttg tcatcgttac gttcgattct atcagtaggt
     4321 gagcctttaa atcctgaagt tataaaatgg gcgaaaaaag tatacggttt aacggtgtta
     4381 gatacttggt ggatgacaga aacaggtgga catatgattg ttaactatcc aacgatggac
     4441 gtcaagcttg gctcaatggg caaaccatta cctggtattc aagctgcaat tatcgatgat
     4501 gcaggtaatg aattaccacc aaatcgaatg ggcaaccttg ctataaaaaa aggctggcca
     4561 tcaatgatgt atcgtatctg gaagaatcca gaaaaatata agtcatattt tattggagac
     4621 tggtatgtat ctggtgattc ggcatataaa gatgaagatg gttacttctg gttccaagga
     4681 cgtgttgatg atgtaattat gacagctggt gaacgagttg gaccatttga ggttgagtct
     4741 aaattggttg aacacgaagc agttgccgaa gcaggaatta ttggtaaacc tgatccggtt
     4801 cgcggtgaaa taattaaggc gtttgttgca ctgagaaaag gatatgaacc aacagacgaa
     4861 ttaaaagaag aaattcgttt atttgttaaa gaaggtttgt cggcacatgc agcaccacgt
     4921 gaaatcgaat ttaaagataa attacctaaa acacgctcag gtaaaattat gagacgtgta
     4981 ttaaaagctt gggaattaaa tttagatgca ggggatttaa gtacaatgga ataatgacat
     5041 gaatgttatt gaagatttct ttcgaagaat aaagggatac aacatatttc atgttaatgt
     5101 ttaaatatta gtttacttta tgataagcaa tataaagagc tgttaacttg tgtcatatca
     5161 tttcgtagaa agcatttgaa aatgatgaca taacaataat ggcatatctt tatattgctt
     5221 tttattttta atatgatctt tggaatatga ctattttaaa taatagaaaa atatagttat
     5281 caatagtatc aagcgctaaa agttgtataa tacaaaactt taataagtga atttattgca
     5341 aaaatgaaag cgctaacccg atttagtcga caagttttta acagttcgtt attatatgaa
     5401 tgtaagtaaa aatttcttag ctacaactta catattataa atgcataaat taaacaaaaa
     5461 ggggcgaaaa aagttgactc atttatcaga tttagatatt gcgaatcaat caacactaca
     5521 accaattaag gatattgctg catcagtagg tatttcagag gatgcattag aaccttatgg
     5581 tcattacaaa gctaaaatcg acattaataa aattacgcca agagaaaaca aagggaaagt
     5641 tgttttagta actgcgatga gcccaacacc agctggtgaa ggtaaatcaa cggttacagt
     5701 tggtttagct gatgcattcc atgagttaaa taaaaacgtt atggttgcat taagagagcc
     5761 tgctttagga ccaacatttg gtattaaagg tggtgcgact ggtggtggtt atgcgcaagt
     5821 cttacctatg gaagatatca acttacattt taatggggat ttccatgcga ttacaactgc
     5881 taataatgca ttgtctgcgt ttatagataa tcatattcac caaggtaatg aattaggaat
     5941 cgatcaaaga cgtattgaat ggaaacgtgt attagatatg aatgatcgtg cacttagaca
     6001 tgtaaatgtt gggttaggtg gacctacaaa tggtgtacca cgtgaagatg gctttaatat
     6061 tacagtagcg tctgaaatta tggcgatttt atgtttaagt agaagtatta aagacttaaa
     6121 agataaaatt agtcgtatta ctattggtta tactagagat cgcaagccag ttacagttgc
     6181 agatttaaaa gtgcaaggtg cacttgcaat gattttaaaa gatgcaatta aaccaaactt
     6241 agtacaatca attgaaggga caccagcatt agttcatggt ggaccatttg cgaatatcgc
     6301 acacggttgt aactcaattt tagcaactga aacagcacgt gatttagctg atatcgttgt
     6361 aacggaagct ggatttggtt cagacttagg cgctgaaaaa ttcatggaca ttaaagcgcg
     6421 tgaagcagga tttgatccgg cagctgtcgt tgttgttgcg acaattcgtg cgttaaaaat
     6481 gcatggtggt gtagcgaaag ataatttaaa agaagaaaat gtagaagcag taaaagcagg
     6541 aattgttaat ttagagcgtc atgttaataa tattaaaaaa ttcggtgtag aaccggttgt
     6601 tgcaattaat gcatttatac atgataccga tgcagaagta gaatatgtaa aatcttgggc
     6661 taaagaaaat aacgtacgaa ttgccttaac tgaagtttgg gaaaaaggtg gtaaaggtgg
     6721 cgttgactta gcaaatgaag tattagaagt cattgatcaa cctaattcat ttaaaccttt
     6781 atatgaatta gaattaccat tagagcaaaa gattgaaaag attgtgactg aaatctatgg
     6841 cggttcaaaa gtaacgttta gcagtaaagc gcaaaaacaa ttaaaacaat ttaaagaaaa
     6901 tggttgggat aattacccag tatgtatggc gaaaacacaa tattcattct cagatgatca
     6961 aacgttgtta ggtgcaccat caggatttga aattacaatt cgtgaattag aagcgaaaac
     7021 aggtgcagga tttatcgtag cgttgacagg tgcaatcatg actatgcctg gtttacctaa
     7081 aaaaccagca gcattaaaca tggatgttac tgatgatggt catgcaattg ggttattcta
     7141 ataaatcatg tcaattgttt aataaagata agtaaatagt ttaatagacc ggactgttgg
     7201 agatgcatta tttcagcagt tcggtttttt gctgtgctaa aaatagattc aatttggcga
     7261 atctaacgac aatgtttgaa ggtggttaat taatgtatat gaagataaaa agtgggcttg
     7321 aagaatagga aagcgatgca atgaatattc catattaaaa aaaaataata aaataggttg
     7381 caatatttaa ttgggatgcg ctacaattaa cactaataat tgatattgat aattattatc
     7441 aattaaatat aatcttatag gagttgttaa caacatgaac aaacatcacc caaaattaag
     7501 gtctttctat tctattagaa aatcaattct aggcgttgca tcggtcattg tcagtacact
     7561 atttttaatt acttctcaac atcaagcaca agcagcagaa aatacaaata cttcagataa
     7621 aatctcggaa aatcaaaata ataatgcaac tacaactcag ccaccaaagg atacaaatca
     7681 aacacaacct gctacgcaac cagcaaacac tgcgaaaacc tatcctgcag cggatgaatc
     7741 acttaaagat gcaattaaag atcctgcatt agaaaataaa gaacatgata taggtccaag
     7801 agaacaagtc aatttccaat tattagataa aaacaatgaa acgcaatact atcacttttt
     7861 cagcatcaaa gatccagcag atgtgtatta cactaaaaag aaagcagaag ttgaattaga
     7921 catcaatact gcttcaacat ggaaaaagtt tgaagtctat gaaaacaatc aaaaattacc
     7981 agtgagactt gtatcatata gtcctgtacc agaagaccat gcctatattc gattcccagt
     8041 ttcagatggc acacaagaat tgaaaattgt ttcttcgact caaattgatg atggagaaga
     8101 aacaaattat gattatacta aattagtatt tgctaaacct atttataacg atccttcact
     8161 tgtgaaatca gatacaaatg atgcagtagt aacgaatgat caatcaagtt cagacgcaag
     8221 taatcaaaca aacacgaata catctaatca aaatacatca acgatcaaca atgctaataa
     8281 tcaaccgcag gcaacgacaa atatgagtca acctgcacaa ccaaaatcgt cagcgaatgc
     8341 agatcaagcg tcaagccaac cagctcatga aacaaattct aatggtaata ctaacgataa
     8401 aacgaatgag tcaagtaatc agtcggatgt taatcaacag tatccaccag cagatgaatc
     8461 actacaagat gcaattaaaa acccggctat catcgataaa gaacatacag ctgataattg
     8521 gcgaccaatt gattttcaaa tgaaaaatga taaaggtgaa agacagttct atcattatgc
     8581 tagtactgtt gaaccagcaa ctgtcatttt tacaaaaaca ggaccaataa ttgaattagg
     8641 tttaaagact gcttcaacat ggaagaaatt tgaagtttat gaaggtgaca aaaagttacc
     8701 agtcgaatta gtatcatatg attctgataa agattatgcc tatattcgtt tcccagtatc
     8761 taatggtacg agagaagtta aaattgtgtc atctattgaa tatggtgaga acatccatga
     8821 agactatgat tatacgctaa tggtctttgc acagcctatt actaataacc cagacgacta
     8881 tgtggatgag gaaacataca atttacaaaa attattagct ccgtatcaca aagctaaaac
     8941 gttagaaaga caagtttatg aattagaaaa attacaagag aaattgccgg aaaaatataa
     9001 ggcggaatat aaaaagaaat tagatcaaac tagagtagag ttagctgatc aggttaaatc
     9061 agcagtgacg gaatttgaaa atgttacacc tacaaatgat caattaacag atgtacaaga
     9121 agcgcatttt gttgtttttg aaagtgaaga aaatagtgag tcagttatgg acggctttgt
     9181 tgaacatcca ttctatacag caactttaaa tggtcaaaaa tatgtagtga tgaaaacaaa
     9241 ggatgacagt tactggaaag atttaattgt agaaggtaaa cgtgtcacta ctgtttctaa
     9301 agatcctaaa aataattcta gaacgttgat tttcccatat atacctgaca aagcagttta
     9361 caatgcgatt gttaaagtcg ttgtggcaaa cattggttat gaaggtcaat atcatgtcag
     9421 aattataaat caggatatca atacaaaaga tgatgataca tcacaaaata acacgagtga
     9481 accgctaaat gtacaaacag gacaagaagg taaggttgct gatacagatg tagctgaaaa
     9541 tagcagcact gcaacaaatc ctaaagatgc gtctgataaa gcagatgtga tagaaccaga
     9601 ttctgacgtg gttaaagatg ctgataataa tattgataaa gatgtgcaac atgatgttga
     9661 tcatttatcc gatatgtcgg ataataatca cttcgataag tatgatttaa aagaaatgga
     9721 tactcaaatt gccaaagata ctgatagaaa tgtggataaa ggtgccgata atagcgttgg
     9781 tatgtcatcg aatgtcgata ctgataaaga ctctaataaa aataaagaca aagtcataca
     9841 gctgaatcat attgccgata aaaataatca taatggaaaa gcagcaaagc ttgacgtagt
     9901 gaaacaaaat tataataata cagacaaagt tactgacaaa aaaacaactg aacatctgcc
     9961 gagtgatatt cataaaactg tagataaaac agtgaaaaca aaagaaaaag ccggcacacc
    10021 atcgaaagaa aacaaactta gtcaatctaa aatgctacca aaaactggag aaacaacttc
    10081 aagccaatca tggtggggct tatatgcgtt attaggtatg ttagctttat tcattcctaa
    10141 attcagaaaa gaatctaaat aattaactgg atatagcata tgtatgatta actttgtaga
    10201 caatgtgaaa ccaattaaat tataaactat tgattggttt aatggctttc ctttagagta
    10261 aataaaaaga acagcagtga gaaattttct aattgaaaat aatcttactg ctgtttttaa
    10321 tatttggatt cattgttgtg gttactttaa aaagtgagca tcaattaacg cttttttcga
    10381 tttaacaaat gtgatttaat atcatatttt aatgcgtcgt tgtattcttt ttcagtgatt
    10441 tgatcttcga ttaacatacg ctttaataca taatgttgtc tttggatact atatttcaaa
    10501 tctttatccg attttaatgt tccatctttt tcgtagggtg tatagccata agggctttgc
    10561 aacaaaccga taaggtatgc agattgtgca attgataaat cttttggtgg aataccaaac
    10621 aaactatatg aagcggatgc aattccggaa atattagcgc cattataatc tctaccgaag
    10681 ggaactatat ttaaatatgt atatataatt tcatcttttg agagtaggtg ttctaatcta
    10741 attgctaggc gaagttcatt tgcttttcta ctatatgttt tttcgttggt aagaacttga
    10801 tttttaacaa gttgttgtgt aattgtgcta ccacctgaac tttgatcagt attaaaaata
    10861 tcttgtatca ttgctcttaa aatcgccttt ggtaagatgc catcatgttt ataaaataaa
    10921 gtgtcttcag atgacgttaa tgctttaatg acatttggac ttgatgtttt agggcctata
    10981 atgagtgagt tttgagaatg gttatactca tataataaat ttttgttatt atgatctaat
    11041 aattcatcgc caggtatttg tcgaactttt tttattaaag catcatctga taatgagtcg
    11101 gacgttttag ttaaatgatg aaaatataaa gacatcgcaa tcacagcgat agcaacgatg
    11161 gcaatgaaaa tcataaatat aaaaacaatg acatgtttga tagctttata tatcttttca
    11221 aaatgatata tacgatgatt caattgatga ttgttgtctt gattcgtcat ctttaacctc
    11281 ctaaattacg tatagtatac catatttaag caatttgact tttattttaa cgttaattat
    11341 aattagggaa aatgcataaa gaataggata aaaggacaag taatattatt tgaaatcatc
    11401 agagagctag tggtcgctgt gaactagtgt ttttaataat atgaatacac ctttataact
    11461 aacatgtcct acgtttatta atgaacgtat cattaataga gtgaacatta tcttatgatt
    11521 atgtttaagt ttggtggtaa cgtgcaatcg cgcccttaca tatttgttgt aagggttttt
    11581 tttacgttca aaaaacttta aagtatttta acaagtattc aataaataat tataaatagg
    11641 aggaaataat aatgacgaat gtattaattg aagatttaaa atggagaggt cttatttatc
    11701 aacaaactga tgaacaaggt attgaagatt tattaaataa agaacaagtg acgttatact
    11761 gcggtgccga tccaacggca gatagtttac atattggtca cttactacca ttcttaacat
    11821 taagacgttt tcaagaacat ggacatcgtc ctatcgtttt aattggcggt ggtacaggta
    11881 tgattggtga tccatcaggt aaatcagaag aacgtgtgct acaaacagaa gaacaagtag
    11941 ataaaaatat cgaaggtatt agtaagcaaa tgcacaatat ttttgaattt ggaacagacc
    12001 atggtgcagt gcttgttaat aatagagact ggttaggaca aatctcatta attagctttt
    12061 tacgtgacta tggtaaacac gtcggcgtta attacatgtt aggtaaagat tcaatccaaa
    12121 gtcgtttaga acatggtatt tcatatacag aattcacata cacgatttta caagctattg
    12181 atttcggtca tttgaataga gaattgaatt gtaagattca agtaggtgga tcagatcaat
    12241 ggggtaatat cacaagtggt attgaattaa tgcgtcgtat gtatggtcaa acagacgcat
    12301 acggtttaac tattccgctt gtaactaaat cagatggtaa gaaatttggt aagtctgagt
    12361 caggtgctgt ttggttagat gctgaaaaaa caagtcctta tgaattttat caattctgga
    12421 ttaatcaatc agacgaagat gtaattaaat tcttaaaata ctttactttc ttaggaaaag
    12481 aagaaattga tcgcttagaa caatctaaaa atgaagcacc gcatttacgt gaagctcaaa
    12541 aaacattagc tgaagaagta actaaattta ttcatggtga agatgcatta aatgatgcaa
    12601 tccgtatttc acaagcatta tttagtggtg atttaaaatc attatcagcg aaagaattaa
    12661 aagatggatt taaagatgtg cctcaagtga cattatcaaa tgacacaaca aatatcgttg
    12721 aagtccttat tgaaacaggc atttctcctt ctaaacgaca agcacgtgaa gatgttaaca
    12781 atggtgcgat ttatattaat ggtgagagac aacaagatgt taattatgct ttagcaccag
    12841 aagataaaat tgatggcgaa tttacgatta ttcgtcgcgg taagaaaaaa tacttcatgg
    12901 ttaactatca ataaatataa ttgcatagct aaataaatta gagcctactc atattcattc
    12961 ctaagaatgt aatgagtagg ctcttaatgt acttttctgt ctgtaaatta tctaaagaaa
    13021 tctctatcgc cttggccagg tgattgacgt tctgatcggc tttgacgttt tggttgttct
    13081 ttttgttgtt ttaatttcac tttaacttct tttgttttac catcacggat aacggtaaca
    13141 gtgactgatt caccaggttt tttattttca tataaatagc ttcttaaatc aacatcatct
    13201 ttaattttct tgccatcaat ttctgtaata atatcacctt ttttaagatc aatatcacta
    13261 tcagctttgg cgacataaat accgtcttct ctatcagtat gaagttgctc gcgctcttct
    13321 tcaggaatat ctttcaaatt aattaaacca ataccaatcg aagggcggtc aattttacca
    13381 tgttttacaa gttgttcaat tgttacttta acttcattac ttggaatagc aaacccgata
    13441 ccttcaactt gtgtcgcagc aattttcatt gagttaatac caactaaatt accattaata
    13501 tctactaatg cgccacctga gttacctggg ttaatagcag catctgtttg aagaacgctg
    13561 acttttgtat tgccaccagt tgtctcagcg tcaatagtac gttcgcttgc tgaaatgata
    13621 ccagatgtta cagagttagc aaattgtaat cctaatgggt tacccattgc gaatacgcta
    13681 tcgccagttt gtacttttga agagttggcg aattgaatcg ctttaatacc ttttgtattt
    13741 tcaattttaa gtacagcaat atcagttact gcatctttac caactaattt cgctttaact
    13801 tgttttttat tatgtaattg gactctaatt tcatttgcgc catcaataac atgattgttt
    13861 gtaacgatat aagctgaatt gttgtttatt tgatagataa cacctgaacc tactccagct
    13921 tcagatggtt tagatgattt gccttttaat aagtcgtcta cacttgatgc tttttgcatg
    13981 ttaataactc caacaattgt aggggagaca gattttatca tttcatgaac ggtaccgaat
    14041 ttcttgcttt gaccgtctaa ttgattgcca cctttattat ttgttgtctg aacagttgaa
    14101 ccatctttat ttaaaattgt actatttaat actttgccta taccaagtac tagaagtgca
    14161 ccaataattc cagcaatcaa tgcaacgatg actgttttaa accatggaaa tttaggtctt
    14221 ctgtatctag gtgtttggct atggtttgtt gtagaatgat ctgtatgatt aaaatctgac
    14281 atacttaacc tccattatat gatttatata tgctttaatt atgtcttttt tttataatta
    14341 ttgctagtaa tacactatac aactttagat gtattttcag actatcttgt tctagtaaaa
    14401 atatagtgat aaaatctagt tgttataaac aaataagtta agtaggtggc acaatgtatt
    14461 cagtgattag taagattttg aatttcattt tagttaaaat gtcaaaatca ttatacgtaa
    14521 tcggtaaaga taatattcct aaggatagta aatatgtcgt aacttgtacg catgaaagtt
    14581 ataacgaagt cattatgcta ggtatggcat tatatcctaa tcaaattcat tatatggcta
    14641 aaaaagaatt attcaaaaat aaatggattg gtaaattttt aacgtcttta aatgcatttc
    14701 ctgtagatcg tgaaaatcca ggacctagta ctttaaaacg cccaatcaat ttgttgaaag
    14761 ataataagac agttggtatc ttcccaacag gttctcgcac atcgcaagaa ggtgcacctt
    14821 tgaaacgtgg tgcttcaaca attgcaatgt taagtaagtc accaatatta cctgttgctt
    14881 atgttggtcc tactaagatt cacggtcttt taacaggaca agcatacata aatattggta
    14941 aaccaatcga tattaacgat ttacctaaag atttaaaacg aaacgagcgt atcgattata
    15001 ttacaaaaga gattgaaaca cgaacagcaa agttacaaca agaattgcat gaaattgtaa
    15061 aaagtttata aaataacttg aggtagaaat atagctggtt agtaagccta ggtgactata
    15121 acggagtatc gaagtagtta ttctagttac attagtaatc tttatatttc taccttattt
    15181 taagaaggat gacattcaat gtataaattt ttccaaaatt tagggcgttc gttaatgtta
    15241 ccagttgcca ttttaccggc tgcagcaatt attgccggaa ttggtaatac gttaaatgca
    15301 cttcatgcga cgccgaaaat cgcaatgttc tttactacgg taggtactac gattttagag
    15361 caattaggta ttttatttgc aattggtgta gctataggaa tggcaaagaa aaatgatggt
    15421 gcagtcgcgc ttgcagctac gttaggatat tttttagtta cagttgtatt atcaccaatg
    15481 aaattagcgc ctttattagg aatgaaagct tcggaaataa attcagcttt tgaaaagatg
    15541 aataatggca atgtctttgt tggtattgta ataggtttaa tagcagctta tgcatacaat
    15601 aagtttagtg aaatagaatt accattagca ttatcatttt ttagtggtaa acgtctagtt
    15661 ccaattatga ctgcatttta ctgtacattt ttagttgtca tattgttatt cttatggcca
    15721 ctactttatt catggattgt aaaatttggt gaatctatag ttggtttagg atcgtttggt
    15781 gctttcatat atggtgttgc taacagacta ttaattccta caggattaca tcatgcactg
    15841 aatagtgtgt tctggtttga tacgattggt attaatgata ttggtaaatt ccaaagtggc
    15901 aaagatgcta taaaaggtat tacaggacgc tatcaagcgg gattcttccc aatcatgatg
    15961 ttcggtattc cagcggctgc gttagcaatg tatcatacag ctaaaacgac tcagaaaaaa
    16021 caagtttacg gttggttcct agcgagttca gttgcagcat ttttcgttgg tgttacagag
    16081 ccgattgagt ttgcatttat gtttgttgcg ccaatcttat atgttgttca tgcgttatta
    16141 acaggattat ctttatttat cgcagctaca ttccattgga cagctggatt ttcatttagt
    16201 gcaggtttga tagattatgt gttatcacta attaatccgg tatcaaatca tccattgatg
    16261 ttgcttgttc aaggtgttgt atttttcatc ttatactatg tgattttccg tgtagtaatc
    16321 caagtattta acttgaatac gattggtaga ggtgaaaatg aattagttga tccaacagtt
    16381 gtaaaagata atattgctcc tggtgaaaat gatattaaac aaagtaaata tcatcaacat
    16441 gctatacaaa tattagaagg tttaggtggt caagagaata ttgttaattt aactaattgt
    16501 gcaacaaggt tgcgtctaga gttaaaagac acatctatta ttgatcaaca gaagattaaa
    16561 aacgccggtg cagttggtgt tactgttaat ggtaaacatt caactcaagt tattgtcggt
    16621 acacacgtac agcaggttgc tgatgaaatt gaaaaacatt tataaaattt aaacagctaa
    16681 agcgcaaatc caacttcaat agggtttgcg ctttttttag tcaaatagta gaatgattta
    16741 tatctgaatt ttcagaaatg gagtggggat tgtgaagaaa attttatttg atgttgatgg
    16801 cgttttttta agtgaagaac gttgtttcga tgtctctgca ttaacggtat atgaattatt
    16861 aatggataag tgttatttag gtcttcattc acatatagat tgggaaactt tgactgataa
    16921 cgatatacaa gacattagga atcgaatttt tcaaaaagat aagattttaa acaaattaaa
    16981 atcgctaggg ctaaattcaa attgggatat gttatttatc gtttttagta ttcatttaat
    17041 tgatattttg aaaaagttat cacatgatga aattgaggca ttcatgtatc aagatgagcc
    17101 tgtcgaattg aagttgcaaa atataagcac aaacttagca gactgtttta atttaaatga
    17161 acaactacct ttacaatttt tggataatgt aaaagttggt aaaaataata tttatgctgc
    17221 tttggaagag tttgcaacaa cagagttaca tgtttcggat gctactttat ttagtttgaa
    17281 gggtgcatta tggacgttag cgcaagaagt ttatcaagaa tggtatttag gatcgaagtt
    17341 gtatgaagat gttgaaaaga aaatagcacg aactactttt aagacaggtt atatttatca
    17401 agaaattatt ttgagaccag tagatgaagt taaggtactt ctgaatgatt taaaaggtgc
    17461 tggtttcgaa ttaggtattg caacaggtcg tccttatact gagactgttg tgccatttga
    17521 aaatttagga ttgttaccat attttgaagc tgattttatt gcaacagcaa gtgatgtttt
    17581 agaagcagag aatatgtatc cgcaagcacg accattagga aagccgaatc cttttagtta
    17641 tatcgcagct ttatatggta ataatcgcga taaatatgaa tcttatatca ataagcaaga
    17701 taacattgta aataaagatg acgtatttat agtaggcgat tcgttagctg acttattaag
    17761 tgctcaaaaa ataggtgcaa cgtttattgg aacattaaca ggtttaaaag gtaaggatgc
    17821 tgcaggtgag ttagaagcgc atcatgccga ctatgttatt aatcatttag gtgaacttag
    17881 aggtgtacta gataatttgt aatttgattg ttgtttgaca gcacaacttg aagtgaatga
    17941 ttgaaccaaa ggtttcatat tgagttacaa tgaaattaat aatgaaaaaa tgccaagaag
    18001 caatggaagt aatccaatgt cttcttggca ttttgaattt acataaattg tttatgactg
    18061 taccgtcaat tcagttgtga aaatttgatt gtattcacca acttgtttaa gttcatcaat
    18121 tatattgttt gaaacaggtt gatcaacgga taaaatcatt agcgcatctc cgcccgcttc
    18181 agttctacct aaagtcatag atgcaatgtt gatattgtat ttacctaaca atgcgccagt
    18241 ttttcctacc atacctggag tatcattatg atatgacaca atttgatatt gatttggctt
    18301 taagtctaca gaaaaattat taattctaac aattcttgga ccgaaacctg taaagacaga
    18361 agcgccaact ttaacggaat cgctatcgtt tgatagttct acctctaagt agttactaaa
    18421 acctgtctct gctttattat tttcaatatt taatgtcact tgttgttgat ttaacaacat
    18481 tagagcattg ataatattga cttcaggacc aagatcatct tttaaaatat gagtaataat
    18541 tgtacgtgtt aataaactac tatcaataga ggctaagtcg ccaccgtacg taattttaat
    18601 agagcttggt gcattgtaca ttaattgaat agctaattca ccaactgttt ggcttaaatt
    18661 gatgaatgat tttacagtat catctatatt gcttaagtcc attttaggtg cattcactgc
    18721 atgcgttaca gtaccatcaa ttaaaatttc gatgatttca tttgaaacag aaattgccac
    18781 tttttcttga gcttcgactg ttgaagcacc caaatgaggt gtaacaataa ttttatcatg
    18841 tgcaacaaga ggcgagtcag ttgcaggttc atgttcaaac acatcgatag ctgcccgact
    18901 aatttgtcct tcgtctaatg cttttattag cgccttttca tcaataatac caccacgtgc
    18961 cacattgatt atttgcaaac taggttttgc tttggcaaaa aagtcagcat taattaagcc
    19021 ttttgtttta ggtgttagtg gtgtatgtaa tgtaacgaaa tcagaatgtt gggcaatctc
    19081 atcaactgtt gccttcgtaa tgcttaaaga ttttgctttt tcatccgtta agtaagggtc
    19141 aaaagctagt attttcattc cgaaactttg cgcacgttta gcaacaccta aaccaattct
    19201 accagcacca atgacaccta atgttttatg ataaagctca gtacctttaa atgcatttcg
    19261 attccattct ttatttgtaa gtgattggtg tgcttgcgga atatttcgtg ccattgataa
    19321 taacattgcc agtgtatgtt cagtagctga aatcgtatta ccatctgggg cattaataac
    19381 taaaatacct tttaatgttg cagcattaat attaatatta tctacaccaa caccggcgcg
    19441 tgcgattact tttaaagaat cagcagcatt tatgatattt tcagtaaccg tagtttgact
    19501 acgaacgatt aaagcatggt atgaaggtat aattttgatt aatgcttctt cggacaagcc
    19561 agtttgaata tctacattga attgttcgtg atctaataat gctttgatac catcttttga
    19621 tatgggatct gcaacgagta cattaaattg cttcatgaat aacctccata tattttgata
    19681 tacctttacc gatatagtta actttacggt gttcagttaa aataatttct aaagcagata
    19741 ctaccgataa aatatcgaaa ggactaattt tccccatatg accaattctt aaaatttgac
    19801 ctttaagatg gccttgacca ccagcaattg ttattttaaa ccgattttta agttcatctt
    19861 tgattatttt cacttcatca tttgtattag gtttgaatgc tgtaaccgtt ggagatgcat
    19921 ctttatcatt gactaataaa gttaaatcta atgcttttaa ggcgcttctt aatgcatttc
    19981 taattgcata gtgtcgtgct attacgtgat tgaaaccttc tgcttttacg gtttcaacgt
    20041 atgcatttac acctctaaat aaagacacat ttggtgtgaa cggtgtagaa ttgtcagctt
    20101 gtgacgaaat gtatttattt aagtctagat aaaattttgg cgtagttact tctttgaaac
    20161 gttcttttgc acggtggcta taagctacaa aagctaatcc tggaggtaac ataattgctt
    20221 tttgactacc agaaacaagt acatcaattt tatctttgtt aatgtcaaca tcaacagcac
    20281 caatgcaact tacgccgtca actacaaaat aaatattact attaaattga ttgatggcat
    20341 ttcctaactc gtgaataggg tgtagcactg tcgtagatgt ttcgcaatat tgactaaata
    20401 ctgctttcac ttcaacattt aaagttgaaa ggaaattgat gaaatctttg acatctacag
    20461 cttctcccca tgttacgtca taaatatgca catttttgta ataagtttgt gcaatttgtt
    20521 taaatcggtt accaaaggca cctgaaacaa tgacaacgaa gtgatcttca gggtttacaa
    20581 tgttcaacat actagcctcc aagacgcttg taccgctaga tgttaaaata agtacatcat
    20641 tttgactccc aaatattggc tttagacctt gaaatgcttg ttgtgcgatg tcttcaaaat
    20701 ctttagaacg atgaccaacc ataggtgctt gaatttctct cataatggca tcaggtacag
    20761 gggttgggcc aggtgttaat aacaacggtt gatgataata catttatatg cctcctataa
    20821 ataagatttc actattttag caaattttct gaaaatttaa aagcctaaaa aagtgataac
    20881 gctatattgt cgaacaaacg ttatcactta aagattttat ttggcctgaa tgatgggata
    20941 aatttttatg tccacattat ttcttacagc atttgaaatc atgcaattat tatctgcaat
    21001 tgttatcaat tttggtaatc gcttttctaa ttgtgctatt tgatcacttg gaatttgaat
    21061 ttgaggatgg tgcacaattt ttgacatact gaattttccg ttatttaaac aagctgttcc
    21121 aatcgattgt tgttcaattg aaatatctgt gaactttgca cgttcaagag tagctgctaa
    21181 tgagatgata taacatgatg aagcggctga tactaacaat tcatcgggat ttgttcctaa
    21241 accaacacca cctaaggaag caggtataga aatattctct gaaagtatgt cgccttgaac
    21301 gtttccgaca ttgttacgac caccttgcca agaagtttgg actttaaagt catgttgatg
    21361 caattgcttt aacctccaat ataattgtga tagtttaatt ttagaatagt ttatcaaagt
    21421 ttaacagaaa ggtgacttat caatgactct gaataaactg aaagatgaat tacaaattgt
    21481 ttcgcaccgt ggattgccga gtgattttcc tgaaaataca atggtcggtt atcgagaggt
    21541 aatggggctc aatgttgcta tgttagaaat agatgttcat ttgaccaaag accaacattt
    21601 tgttgtgata catgatgaaa caattgatag aacatcggat ggtaaggggc gtatttctga
    21661 ttacacatta tcgcaattaa aatcatttga ttttggtagt tataaagatg ttgcttttaa
    21721 gggagaacga atacccacgt tagatgaagt actttcatta tgcttaaagt atgataaaaa
    21781 gctattgatt gaattgaaaa gtccgaattt atatccagaa atagaatgta aattgctggc
    21841 atttttggaa gaaaagaaag tcgatgcgac acaagtagtt atacaatctt ttgatatcga
    21901 atgcatcgaa aagttaaaca cattgggcag tatatatgaa ttaggtgtgc tttgtagtaa
    21961 acgtaattat tggtataaaa agccaaactt ttcaagaatt gctcaaattg ctagttatgt
    22021 taatcctaat tatgcattag tgaccagaaa atttgttgat aaagcacatc atcatcaatt
    22081 acaggttatg ccatacacag ttaataaatt gaaaactgga gaaaaattaa gacaaatggg
    22141 tgtagatgga ttaattacag ataatccgaa attatttatt aagtgagacg atgaatggta
    22201 atttgataga gggatgagga ttcgtatatc gcaattgata attttaaaaa ctaatgatga
    22261 aatactaaag ataattaaac aaataagact tgtaaattct aagttgattg taaaataggg
    22321 tagctccata taattttgaa tctatcattt ttaaattaat aaaaaaaaca cccacaattg
    22381 tgggtgtttg tattgaacgt attatcttga gtagtactca acgattaatt gttcgttaat
    22441 ttcagcaggt aattcgctac gttctggtaa acgtacgaaa gtaccagtta agctgtcagc
    22501 atcaaagttt aagtactcag gtacgaaatt gttgatttca actgattcaa cgatgatgtt
    22561 taatttttga gatttttcac gaactgaaat tgtttgacca ggtttaacag aataagatgg
    22621 aatatcaaca cgtttaccat ctactaagat atgaccgtgg ttaactaatt gacgtgcttg
    22681 acgacgagta cgagctaaac ctaatgaata aacaacagcg tctaaacgac ttgctaataa
    22741 gatcatgaag ttttcaccgt gtacaccgaa ttttttacca gcgatgtcaa atgtgttacg
    22801 gaattgtctt tcagtcattc catataagta acgtaatttt tgtttttcac gtaattgtaa
    22861 accatattct gataattttt tacgttggtt tggaccatgt tgtcctggtg cgtaaggacg
    22921 tttttctaat tctttaccag taccgcttaa agagatacct aaacgacgag attttttcca
    22981 gtttgaacct ctgaatcgag ccataataag actcctcctt taattctttt ttgttgttat
    23041 gaataaacaa aaaagagtgt tgcatgctta ctaagatata ttgttttatg tgtcttcacc
    23101 ttatagcgac agttacgtga cacgtccgca ttgggaacaa catagcgctc aataatatac
    23161 aactgctact ttcgttaatt cacacaaaga ttattgtaac attttcctgc taactgtcaa
    23221 cactttattg aaaatgctga tgacctttta tgcgagttgc ttttcaataa ttttaacaat
    23281 tgcttcaaga tgttctttgt cattgtcatc aaatcgatca gttatagggg catcgatatc
    23341 taagacgccg ataattttat catctttgaa aattggaacg acaatctcag acttactatt
    23401 agcatcacaa gcgatatgtc cttcgaattg atggacatca gctacaactt gtgtacgacg
    23461 ttctgaaacg gctgtaccac atacaccttt tccaattgga atgtggacac aagcggggtg
    23521 tccttggaaa ggtccaagta taagttcatt ttgttccaat aagtaaaagc cgacccaatt
    23581 aatttgatct agattatcat ttaataaggc agacatgtta cttaaaatag caatcatatg
    23641 atgttcatct tcaataaggc ttgctgcttg tttctttaat aatgtgtagt ttgttgggtt
    23701 aattgttgtc atatttaaaa cctcttattt tcatagattt tataatagta tagaatttcg
    23761 aaaatattat ggcaataaat tcattttata atttatcctt aagcaaaatt acgttataat
    23821 agtaatgata aattaggagg agaagcatat ggtgttatat atcattttgg caataattgt
    23881 gattatattg attgctgtag gtgtattatt ctatttacgt tcaaataaac gacaaattat
    23941 tgaaaaagca atcgaacgta aaaatgaaat tgagacgtta ccttttgatc aaaaccttgc
    24001 acaattatct aagttgaatt taaaaggtga aacaaaaacg aaatacgatg caatgaaaaa
    24061 ggacaacgta gaaagtacaa ataagtatct agctcctgtg gaagaaaaaa tccataatgc
    24121 tgaggcttta ttagataaat ttagtttcaa cgcatctcaa agtgaaattg atgatgcaaa
    24181 tgagttgatg gatagttacg aacaaagcta tcagcaacaa ttagaagatg taaatgaaat
    24241 tattgcgtta tacaaagata atgatgaatt atatgacaaa tgtaaggttg attatcgtga
    24301 aatgaaacgt gatgttttag caaatcgtca tcaatttggt gaggcagcaa gtctacttga
    24361 aactgaaatt gaaaagtttg agccaaggtt agagcaatat gaagtactaa aagctgatgg
    24421 taattatgta caagcgcaca accatatagc tgccttgaat gaacaaatga aacagctaag
    24481 atcttatatg gaagaaatac cagaattaat tagagaaact caaaaagaat tacctggtca
    24541 attccaagat ttaaaatatg gttgccgtga tcttaaagtt gaagggtatg atctggatca
    24601 cgtaaaagta gacagtacat tacaaagctt aaaaacagag cttagtttcg ttgaaccatt
    24661 aattagccgc ttagaattag aagaagctaa tgataaacta gctaatatca atgataagtt
    24721 agatgacatg tatgatttaa ttgaacatga agttaaagct aaaaatgatg tcgaagaaac
    24781 aaaagatatc attacggata acttattcaa agctaaagac atgaattata cattgcaaac
    24841 agaaattgaa tatgtacgtg aaaactacta tataaatgaa tctgatgctc aaagtgttcg
    24901 tcaatttgaa aatgaaattc aaagtttaat ttctgtatat gatgatattt taaaagaaat
    24961 gtctaaatct gctgtgcgat atagcgaggt tcaggataat ttacaatatt tagaagatca
    25021 tgtcacagtt attaatgaca aacaagaaaa gctacaaaat catctgattc aattgcgtga
    25081 agatgaagca gaagcagaag acaatctgct acgagtccaa tcgaagaaag aagaagtgta
    25141 tcgtcgatta cttgcttcta acttaacaag cgttcctgaa aggtttatca tcatgaaaaa
    25201 tgaaattgat catgaagttc gtgatgttaa cgaacaattt agtgaacgtc caatacacgt
    25261 taaacagtta aaagataaag tgtctaaaat tgtgattcaa atgaatacat ttgaagatga
    25321 agcaaatgat gttcttgtta atgctgttta tgcagagaaa ttaattcaat atggaaatag
    25381 atatcgtaag gactatagca atgttgataa gagcttaaat gaagctgaac gattatttaa
    25441 aaataatcgc tataagcgtg cgattgaaat tgcagagcaa gctcttgaaa gtgttgagcc
    25501 aggtgttact aaacatattg aagaagaagt tattaagcaa tagaaactag tatgtagtta
    25561 tacttaaata atatgagcac tctgtcaaat tggactgatg agtttaataa ttgaagttag
    25621 ccaacgatac gttgtctagc ttctttttta tatggataaa tgaaagggac aataaatata
    25681 aatagcaatt gtttaaagat aaacgtaatc aaatgtgttg ttttaattaa tataagtagt
    25741 ga
//
LOCUS       DAMZLE010000025        25058 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMZLE010000025 DAMZLE010000000
VERSION     DAMZLE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.025"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..360
                     /locus_tag="POB92_000917"
     CDS             <1..360
                     /locus_tag="POB92_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6578434.1"
                     /translation="KTPVGYLISFNTQSLSQSVAENIIRVYTVMTIILFVVIHAIILM
                     GIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVETKTEKAQDSLND
                     NTTKTMTSDKPEDNQPK"
     gene            complement(793..897)
                     /locus_tag="POB92_000918"
     CDS             complement(793..897)
                     /locus_tag="POB92_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578435.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            1577..1735
                     /locus_tag="POB92_000919"
     CDS             1577..1735
                     /locus_tag="POB92_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDI6578436.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(2394..3251)
                     /locus_tag="POB92_000920"
     CDS             complement(2394..3251)
                     /locus_tag="POB92_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6578437.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASPYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(3319..4101)
                     /locus_tag="POB92_000921"
     CDS             complement(3319..4101)
                     /locus_tag="POB92_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578438.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDEEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPMLAMIYVTHFIEEITSNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(4333..6138)
                     /gene="glmS"
                     /locus_tag="POB92_000922"
     CDS             complement(4333..6138)
                     /gene="glmS"
                     /locus_tag="POB92_000922"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI6578439.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6819..8357
                     /locus_tag="POB92_000923"
     CDS             6819..8357
                     /locus_tag="POB92_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDI6578440.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            8392..10524
                     /locus_tag="POB92_000924"
     CDS             8392..10524
                     /locus_tag="POB92_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6578441.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDIDGNTSFENEQTSYLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDAINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMVLITVLEEPLILPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10536..10970
                     /locus_tag="POB92_000925"
     CDS             10536..10970
                     /locus_tag="POB92_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6578442.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            10970..12076
                     /locus_tag="POB92_000926"
     CDS             10970..12076
                     /locus_tag="POB92_000926"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6578443.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(12396..19901)
                     /locus_tag="POB92_000927"
                     /pseudo
     CDS             complement(12396..19901)
                     /locus_tag="POB92_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(20187..21542)
                     /locus_tag="POB92_000928"
     CDS             complement(20187..21542)
                     /locus_tag="POB92_000928"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI6578444.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21569..22501)
                     /locus_tag="POB92_000929"
     CDS             complement(21569..22501)
                     /locus_tag="POB92_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDI6578445.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(22503..23312)
                     /locus_tag="POB92_000930"
     CDS             complement(22503..23312)
                     /locus_tag="POB92_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDI6578446.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(23501..24409)
                     /gene="rocF"
                     /locus_tag="POB92_000931"
     CDS             complement(23501..24409)
                     /gene="rocF"
                     /locus_tag="POB92_000931"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDI6578447.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPVVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(24547..24619)
                     /locus_tag="POB92_000932"
     tRNA            complement(24547..24619)
                     /locus_tag="POB92_000932"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24584..24586),aa:Lys,seq:ttt)
     gene            complement(24623..24697)
                     /locus_tag="POB92_000933"
     tRNA            complement(24623..24697)
                     /locus_tag="POB92_000933"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24663..24665),aa:Gln,seq:ttg)
     gene            complement(24707..24790)
                     /locus_tag="POB92_000934"
     tRNA            complement(24707..24790)
                     /locus_tag="POB92_000934"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24754..24756),aa:Tyr,seq:gta)
     gene            complement(24820..24895)
                     /locus_tag="POB92_000935"
     tRNA            complement(24820..24895)
                     /locus_tag="POB92_000935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24860..24862),aa:Val,seq:tac)
     gene            complement(24903..24974)
                     /locus_tag="POB92_000936"
     tRNA            complement(24903..24974)
                     /locus_tag="POB92_000936"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24939..24941),aa:Glu,seq:ttc)
     gene            complement(24976..25050)
                     /locus_tag="POB92_000937"
     tRNA            complement(24976..25050)
                     /locus_tag="POB92_000937"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25016..25018),aa:Asn,seq:gtt)
BASE COUNT         7389 a         4501 c         3996 g         9172 t
ORIGIN      
        1 aaaacacctg ttggttactt gatttcattt aatacgcaat cactctctca aagtgttgca
       61 gaaaatatca taagagttta tacagtaatg acaattattt tattcgttgt tattcatgca
      121 atcattttaa tgggtatcgt tcaatattac ttaaaacgtg gtcaaaaaat tactaaagat
      181 aaagtaaaaa cagctgacaa gaataaaaaa gttgtcactg aaccaaaaaa tacaaaagta
      241 gaaaatgata aagtgacaac atctgttgaa acaaaaacag aaaaagcaca agattcatta
      301 aatgataata caacaaaaac aatgacttca gataaaccag aagacaatca accaaaataa
      361 attttagagc cccttacaac tttagttgtt aggggctctt atgcatgcgc ttagcgcagg
      421 cgctcctact tttcaacttt agttgttagg ggcttctttc taaaataact aagtgaatta
      481 tctatatatg aagaggtctc caatactttt tatataattc gtgattacat tctatacttt
      541 taattgtatt ttctgctgta tggaaagttg gttttattaa ttctgattat gttcagaact
      601 acaattcata agaaaaatgt gttaaaatat atttgtagta agtagaagca aaagatgaaa
      661 atctttaact cttgaaacac aaaaagggca acactcggaa acatgttacc ctaatgagcc
      721 cgttaaaaag acggtgacct tatattttat ttaaaaatag ccttcaaaat gccggtcaaa
      781 gcgaatagaa ggttattttt tatggtcttg agtactaatc aatactaaac caatgattgt
      841 taatacaacc atcagcattt caaaatcaga catgctagtc ctttctagga gtaacaacta
      901 taaccatagg catcacccct ttattaagag attagccacc atctatccaa cttgctcaat
      961 aaatattata acatgcacac ttttaaacag taattcatag tataagtttt ttcatttctt
     1021 aatcaactcg ataaacagtt gagtgacata cccttgtcta actagctatt tcttcccctg
     1081 ttatcgtttg ttcatcatat aaaaatttaa tttctcattt tttattctct ggtaaagaag
     1141 agcaccaacc ttttcggctg atatgctccc ttcaaaatag acattgaaaa atgaaaactt
     1201 tgaaagagaa atcgtcagtc tattattgat aagtattatc ctatcctcga aaaattactt
     1261 tcagacacta gtgaacaaaa attttactat aagcgtatac tttggcaata ttcgaaggaa
     1321 aaacatggat taacctgtgc atattataca tttagagctt atatacttaa acatgatgaa
     1381 tttactcctt atattatgaa atgttatcaa tagatgtcgc ctaaagatac aacaagattc
     1441 gagacaaaac ctgatcatca agcacagttt gactggaaag aaagcattaa ctttaaaacg
     1501 aaagataatc aaaggattcc actatatata ggtgtgctac tactttatta ttctcgtttt
     1561 ataatcatgc ataacaatga gtaaatcaag tgatgcctta tttaattcat taacacaatc
     1621 gtttgagcta atagaaggtg tatataatga acttgtaacg gataacttga aaatagtcat
     1681 gtataagcca agaaaagaaa gtacaagtgg tcgaataaat cgtagattta aataattcac
     1741 tgatggtttt aaatttcaag taaaaccttg tatttccggc ctccctagaa aaagataaag
     1801 ttgaatcaat aacaaaaata ttagatgaaa ttaatgctta ccaagacgaa ttgcatttgg
     1861 aagagattcc taagtttttt taatcttaat gaacgattta actactaagt tcatactagt
     1921 acaggtaaat tgcctattac tgcctaagag aaagaaaaaa ttttcttaca accactacag
     1981 aatgtgaatg taaaaaactc atataaagtg aaacatatct tacttatgga atgaaatgtt
     2041 tgattatccc aaaatcacaa atgcgatttt ggagttgtta ggggctctaa cgcaaataca
     2101 cccctactta aaataacggt agttgttata gtatacttaa atgcataaac catataaaag
     2161 gtgatgctaa tatgacatac ggttcaattg tagctatact ctgtgcccta tttgtgattt
     2221 tcttcattcc atatatggag aagaaagaca aaaagaagaa ataacactag tatctggatt
     2281 actagcatac aaaggagcag taagacattt tctaatagaa aataacttta ctgctccttt
     2341 gtaaattact cttcaactat tgtaaattaa ataataaata actgccgatg ttattaatca
     2401 gctaaaaaat gctttgctaa atattttgct gcgccatcct cttcattcgt gtatgccgtt
     2461 acatctgaag ttaatgcttg gatttcaggg cgtgcatttt tcatagcaac tgtataatga
     2521 ccaaattcaa acattgctct atcattgtcg ctatctccaa taactaacgt ttcttcttga
     2581 tgaataccaa aatgtgcaat catttcttta atgcctgtac ctttatcagt ttgataagcc
     2641 attgtttccg cattaaatct tgatgaattt gaaacactaa tctgtagttg cacatgattt
     2701 tgctttaatt catctctaaa tgctgttatt ttttctaaat tagaactgaa tagataaatt
     2761 tttgaatatt caccttcagg aaacttagtt acccaatcta tcttaccagc aagcgcatct
     2821 tgtcttgaag accattcact atgacttacg ccattaatag gatcttgact acgaatcata
     2881 tctcgcatcc atgtttcatc ttcttttaaa gaaactctat taccttcaaa aggaaatacc
     2941 tcataataaa tttgttggcg cttagctaaa ttagtaattt gttgcacttg agccaatgat
     3001 aaaccatgct tgaagataat ttctccatct acttctccaa ttgttccatt tgaactaatg
     3061 atgccattaa ccgcaaaatc ttgaggtaca agttgatgta tttcagaatg cgaacgtcct
     3121 gttgccaaaa atactttata tcctttctct ctcaatcgat taatgacatc tttcgtatac
     3181 ggtgatgctt gattattttc atgtaaaatt gttccatcca tgtccaaaaa tattgctttt
     3241 acgttatcca tattgccctc cactgtccat tttatcttaa ttaaattttg tcattattag
     3301 ttagtatttg caaaatattt actctaacat tgccatagaa aatcgattat tccatctttg
     3361 aactgctaca tttttctgga aaaatcgtga catgttttca gatgtcaaaa tatcatctat
     3421 taatccttgt tgtacacttt ccccatcttt taatagcaaa attttagtaa agttactagt
     3481 aatttcttca ataaagtgcg taacataaat catcgcaagc attggatatg aatctgacaa
     3541 tgagtcaagt atacttaaca acgattctcg tgcaataaag tctaaaccag ctgctggctc
     3601 atctaaaatt aaaacctggg gttgccccat taaagctcgc gcaatcatca ctcgttgttt
     3661 ttcacctgta gataaataac caatatattg ttgcgcttta gcagacattc caactaattt
     3721 aagtaattga tgtgcttcat tacgtacttc ttcatcaata tcttgataaa caccaattga
     3781 tttaaaagca ccgcttatca ccacatcgat tactctttca ccctcttgaa acttttccag
     3841 taaactatga gatacaaaac ctatatgttg tcgtacagtc tctgcagaat accctacctt
     3901 gcctggcatt ttaccgaaaa ggttaacagt tccagatgtt gcaggctcat acgcatttaa
     3961 aatatttaga agtgttgtct tgccagcacc attcaaccca tataatatcc atttatcacc
     4021 tttagcaatt tgccaagaaa tcttttttaa aattgttttt ccttgcttca ttcgcccaat
     4081 ttgatctaat tgaattaaca tgcgaggtca cccctttatt gctactccat acttctcata
     4141 aaatcatatt aacataacac ccttaattgt cagactattt aaataaataa aacacttcat
     4201 ttttacgcat ttctgccaaa ttaagatgaa gtaaaagcta agtcgaccta aaaaagcacc
     4261 cttctagtcg attaatctaa aagggtgcca tatactttaa ttttaataca tgattgattc
     4321 taaaaaagtg aattattcca cagtaactga tttagcaagg ttacgtggtt tatcaacatc
     4381 taaatctctg tgtaatgctg catagtatga aattaattgt aatgcaacca ctgatactaa
     4441 tggcgttaac aattcatgta catgaggaat gacataagtg tcgccttctt tttcaagacc
     4501 ctccatagaa ataatacatg gatgtgcacc acgtgctact acctctttaa cgttaccacg
     4561 aattgataaa ttaactttct cttgtgttgc taaacctaca actggtgtac cttcttcgat
     4621 taaggcaatt gtaccatgtt taagttctcc accagcaaaa ccttctgctt gaatgtaaga
     4681 aatttcttta agttttaacg caccttctaa acttacgtta tagtcaatag tacgtccgat
     4741 aaagaatgca ttgcgtgttg tttctaagaa atctgtagca atttgttcca taattggtgc
     4801 atcgtcaaca attgcttcta ttgctgttgt tacttttgct aattctctca ataaatcaat
     4861 atctgcttca cgaccatgct cttttgcaac gatttgagac aagattgata atactgcaat
     4921 ttgtgcagta tatgcttttg tagatgcaac tgcgatttca ggacccgcgt gtaataacaa
     4981 tgtgtggtct gcttcacgtg ataaagttga acctgcaaca ttagtgattg ttaatgattt
     5041 atgacctaat ttattagttt caactaatac ggcgcggcta tctgcagttt cacctgattg
     5101 agaaatataa acgaacaatg gtttttcaga taataatggc atgttgtaga caaactctga
     5161 tgcaacgtgt acttcagttg gtacgccagc ccatttttct aaaaattctt tacctactaa
     5221 acctgcatgg tagcttgtac ctgctgcaat aacgtaaatg cggtctgctt ctttaacatc
     5281 attgatgatg tcttgatcaa ttttcaagtt accttctgca tcttgatatt cttgaataat
     5341 acgacgcatt actgctggtt gttcatgaat ttcttttaac atgtagtgtg cataaacacc
     5401 tttttcagca tctgatgcat caatttcagc aatatatgaa tcacgttcta caacgtttcc
     5461 atctgcatct ttaataataa cttcatcttt tttaacaata acgatttcat ggtcatggat
     5521 ttctttatat tcgcttgtca cttgtaacat tgcaagtgcg tctgatgcga taacattgaa
     5581 accttcacca acacctaata ataatggtga tttattttta gcaacataga ttgtgtcttt
     5641 gtcttcagca tctaataaac ctaatgcata tgaaccatgt aataatgaca caacttttgt
     5701 aaatgcttct tcagttgaaa gtccttgatt tgaaaagtat tcaactaatt gaacgataac
     5761 ttctgtatct gtttctgaaa tgaatgatac accttgtaag tattcacctt ttaactcttc
     5821 atagttttca ataacaccgt tatgaactag agtaaaacgg ccatttgatg attgatgtgg
     5881 atgagagttt tcatgattcg gtacaccgtg tgttgcccaa cgtgtgtgac cgattccaac
     5941 aggtccatca aaatcgctac tatcagcaac tttacgtaat tctgcaatac gacctttttc
     6001 tttaaataca gttgtattat catcatttac tactgcgata cctgcagagt cataacctct
     6061 gtattctaat ttttctaaac cttttaataa taattctttg gcattatcat agccaatata
     6121 accaacaatt ccacacataa cgacattttc ctccatattg gaatagtacg cgtaaattat
     6181 gatttattgc cgataattta gattgacaat ctgctttcat aatataaata ggaacatgct
     6241 atcatcgcat tcatccataa caaattaagc atagttattt ttacaactat acaaattgct
     6301 cacactgtac tttccatatt aatatttttt atattcaatt tctggcgatc ttattaactt
     6361 tgtccattaa gtcaccctaa tgttttactt aataagctaa cgaatgagcc acatccggga
     6421 tagcatccgc cgatctattc gatcactatc ctcctcgtct acaaatacat atattgcact
     6481 ctataaaggc cactcatata ttaaccttta atcttcaaat acaaatattt atttgcacag
     6541 gcgctttaac tgtactgccg aactttcccc ctttccatta atcattattg tacaacggtg
     6601 ttgttttgtt ttgcaaatat tttcacaata aaattttaaa aatcctaaaa caattttttt
     6661 gttttacttt ttcaaaatat ctatactgtc acattgatga cactttattt aattttgtca
     6721 catttatttt gacaaagttg atttttgttt atattgagta acaagtaacc tctctataca
     6781 ctatatatag tcacatatat taaaaaagag gtgtaaacat gtcacaaact gaagagaaaa
     6841 aaggaattgg tcgtcgtgtt caagcatttg gatcattctt aagtagtatg atcatgccaa
     6901 acattggcgc tttcatcgct tggggtttta tcgcggccat ttttatagat aatggatggt
     6961 tacctaacaa agatttagct actttagcag gaccaatgat tacttattta atcccattat
     7021 tgatcgcatt tagtggtggt cgtttaattt atgatttacg tggtggtatc atcgcagcaa
     7081 cagcaactat gggggtcatc gttgcattac ctgatacacc aatgttactt ggtgcaatga
     7141 ttatgggtcc acttgttggt tggttaatga agaagaccga ccaattgatt caacctagaa
     7201 cgccgcaagg ttttgaaatg ttatttaata acttttctgc tggtatttta ggatttatca
     7261 tgacgattgc aggatttaaa attttagcac cacttatgaa atttattatg catattttat
     7321 cagtggcagt tgaagcatta gtacatgcac acctacttcc acttgtaagt attttagtag
     7381 agcctgcaaa aattgtattt ttaaacaatg cgattaacca tggtgtattc acaccgcttg
     7441 gtgcagatca agctgcaaaa gctggtcaat caattttata cacaattgaa tctaaccctg
     7501 gaccaggttt aggtatctta cttgcttata tgatttttgg aaaaggcact gcaaaagcaa
     7561 cgtcatatgg tgctggaatt atccacttct taggtggtat tcatgaaatt tatttcccat
     7621 atgtattaat gcgtccttta ttatttatcg ctgttatttt aggtggaatg actggtgttg
     7681 caacttatca agcaacagga tttggattta aaagtcccgc gtcaccaggt tcatttatag
     7741 tttactgttt aaatgcacct agaggtgaat tcttgcacat gttactcggt gtcttccttg
     7801 ctgcacttgt atcattcgtt gtagctgctt taattatgaa gttcactaga gaaccaaagc
     7861 aggatttaga agctgcgaca gctcaaatgg aaaatactaa agggaaaaaa tcaagcgttg
     7921 cttctaagtt agtatcttct gataaaaatg ttaatacaga agaaaatgct agtggtaatg
     7981 ttagtgaaac atcttcatca gatgatgatc ctgaagcgct attggataat tacaacactg
     8041 aagatgttga tgcacacaat tacaataata taaatcatgt tatttttgcc tgcgatgcgg
     8101 gtatgggttc tagtgcaatg ggtgcaagca tgttacgtaa taaatttaaa aaggcgggca
     8161 ttaatgatat tacagttaca aatactgcga ttaatcaatt gccaaaagat gctcaattag
     8221 ttatcactca gaaaaaacta actgatcgtg ctattaaaca aacaccaaat gccatccata
     8281 tttcagtgga taatttcctt aattcaccaa gatatgaaga acttttaaat aatctaaaaa
     8341 aagatgatca agcataataa ttaaataaat taaaaaatgg aggataccgc catgttattg
     8401 agtacacgtg aaaaagaaat gatagcccta ttgattaagt accacggtca atatatcact
     8461 atacacgaca ttgctcagca acttgcggtg tcctctcgta ctattcaccg tgaattaaaa
     8521 ggtgttgaag catatttaac ttcattttca ttaactttag aacgcgcaaa caaaaaaggg
     8581 ctacgcattg ctggcacaga ttctgattta aacgatttga agcaatcgat tgcacaacat
     8641 caaaccattg acttatctgt tgaagagcag aaagtaatta ttatatacgc tttgatacaa
     8701 gccaaggagc cagttaaaca atatagttta gcgcaagaaa ttggcgtttc tgtccagact
     8761 ttagcaaaga tgttagatga tttagagctt gatttaaata agtaccaact atctttatct
     8821 cgaaagcgtg gcgaaggcat ttacttggta ggtactgaat caaagaaacg tgaattttta
     8881 agtcaattaa tggtgaataa cttaaatagt actagcgttt attcagtaat tgaaaatcat
     8941 tttgtctttc attcattaaa tcaaatccac aaagactttg ttgacttaga gcgcattttt
     9001 aatgttgaaa gactattaat ggactaccta agtgccttac cctaccaact taccgaatca
     9061 agttatttaa ctttaactgt ccatatcgtg ctatccattt cacgtataaa aaatggtgag
     9121 tatgtcgcat taaacgatga tatttatgat tctgtacaaa acacatttga acacaaagta
     9181 gcaagcgaac ttgctgataa acttggtcaa atatatgacg tcacgtttaa tcaggcagaa
     9241 attgctttca ttactatcca tttacgtgga gctaaacgaa aaaatcttaa tgatacatca
     9301 ttaaataatc gttgtgaaga aaacaaaatt aaagcgtttg ttaacaaagt agaaatgatt
     9361 tccggtatga catttgcaga tttggatact ttagtagatg gactgacgct acaccttaat
     9421 cctgcaatca atcgtttgca agctaatatc gagacctata atccgttaac agacatgatt
     9481 aagttcaaat atccaagact atttgaaaat gtaagattag ctttaaatga ttgttggcct
     9541 gatttgattt ttccagagag tgaaattgct tttatagttt tacactttgg tggctcgatt
     9601 aaaaaccaag gtaatcgatt tttaaacata ttagtcgttt gcagcagtgg tatgggaact
     9661 agtcgtctat tatcaactcg tctagagcaa gtttttagtg agattgagcg tattacacaa
     9721 gcatcagtca gcgatttgaa gtcactagat ttaagtcaat atgatggcat tatttctact
     9781 gtgaatttag acatcgactc cccctattta acggtaaacc cattattacc agatagtgat
     9841 atcagttatg tcgcacagtt tttaaataca aagtctacgt tccaagagac gcatgataaa
     9901 tcatcaaaca tgattgataa ggatgatgtt catgttgaaa cgaaagatat tgatggcaat
     9961 acatcttttg aaaatgaaca aacttcatac ttaacttcag ttttcgaaaa acatttaagt
    10021 gacgaaaaat cagaacaatt aatgcatcat atgcgttcgg gtttaacttt gcttgattca
    10081 gtgaaaatag ttagtaccga agttaaacag tggcaaacat atatcgcaga ttatctatat
    10141 caatgcgatg caataaacga tccaacgtca ttcgctgaac tactagagca acgattgatt
    10201 gacaatccag gatggatatt aagtccatat cctgttgcga taccacacct aagagacaat
    10261 atgattaaac accctatggt tctaatcaca gttttagaag aaccgttaat attgcctagt
    10321 attcaaaatg acaatcaaac aattaaatat atgatttcca tgtttattcc tgacaatgat
    10381 tttatggcat cactggtaag tgacttgtcc gaatttttaa gtttgaaatt agaatctatt
    10441 gatactttta tggaaaatcc acaggaactt gaaacattat taagaaacaa atttttagaa
    10501 cgaattaaaa aacaatttat ttaggagtgt acaaaatgag cgaattattt agtaatgaca
    10561 atatcttttt aaatgtaaat gttaacagcc aaaatgaagc aattgaaaaa gcaggtaaag
    10621 ccttagttga tagtggtgct gtaacagatg cttatattca agcaatgaaa gatcgtgaac
    10681 aagtcgtatc aacatttatg ggaaatggct tagcaattcc tcatggcaca gatgaagcta
    10741 aaacaaatgt gattcactca ggtttaacat tattacaaat ccctgaaggc gttgactggg
    10801 atggcgaagt agttaaagtt gtcgtgggaa tcgctggtaa agatggcgaa catttagact
    10861 tgttatctaa aattgcaatt acatttagcg aagaagaaaa tgtggatcgt atcgttcaag
    10921 caaaatctgc agaagaaatt aaacaagtat tcgaggaggc agatgcataa tgaaagcagt
    10981 tcactttggt gctggtaaca taggtcgtgg tttcattggt tatattcttg cagacaacaa
    11041 tgttaaagta acatttgcag acgtcaatga agaaatcatt aatgctttag ctcatgatca
    11101 tcaatacgat gttattttag ctgatgagtc taaaacaacg acgcgcgtga ataatgttga
    11161 tgcaattaat tcaatgcaac cttctgaagc gttgaaacaa gcaattctag aagctgatat
    11221 tattacaaca gctgttggtg ttaacatact acctattatt gctaaatctt ttgcgccttt
    11281 cttaaaagaa aaaacaaacc atgttaatat tgttgcttgt gagaatgcta ttatggcaac
    11341 tgatacattg aaaaaagcag tacttgatat tactggccct cttggtaaca atattcattt
    11401 tgctaactca gcagttgata gaattgtacc attacaaaag aatgaaaata tattagacgt
    11461 tatggttgag ccattttacg aatgggttgt tgaaaaagat gcatggtatg gcccagaact
    11521 aaatcatatt aaatatgttg atgatttaac accatatatt gagcgtaaat tattaactgt
    11581 gaatacagga catgcatatt tagcgtatgc tggtaaattt gcaggtaaag ctaccgtatt
    11641 agacgctgta aaagatagtt caattgaagc tggcttacgc cgtgttttag ctgaaacaag
    11701 tcaatatatt actaatgaat ttgattttac tgaagcggaa caagctggtt atgttgaaaa
    11761 aataatagac cgtttcaaca attcttattt atctgatgaa gtgacacgtg tcggacgagg
    11821 tacgttacgt aaaattggcc ctaaagatag aattataaaa ccattaacat atctttataa
    11881 taaagattta gaacgcactg gtttattaaa tacagctgca ttgttattga agtatgatga
    11941 tacagcagac caagaaactg ttgagaaaaa taattacatt aaagaacacg gtttaaaagc
    12001 gtttttaagc gaatatgcta aagttgacga tggcttagcc gatgaaataa ttgaagcgta
    12061 caattcactt tcataattta ttgagctttg tttgaaacaa gaagtttcca acgttattcg
    12121 ttaacaatca gtaataatgt agtagttccg ttgaattaac aatattaaat ttctgaacat
    12181 aaaaaaatac tcccttcaac atagacactt aacttgtgtt atgtatgaaa ggagtatttt
    12241 tgcgttaata atttgtttta ttttcgagcc acagccacct attcaatggc tattggtcat
    12301 tactaaaaca aattcatatt aactgttaga cttggttact tagtaaggaa tatttcccta
    12361 tgaaataact agatgttcac attcttgaat aaattttatt cttcagtttg ttggtctttc
    12421 ttagtgaatc ttctaattaa gaatgccata cctgcaccta gagctaattc agcatatggt
    12481 aaatcgtcat tatgtgacat accagtatct ggtaaagttt tagcttgttg tttaggctta
    12541 ttaacttttc cttgttggtc tgattttgca tttgctttgt tttcttcagt cttagttaca
    12601 ttaagcatat cttgatgcat actatgacta ccattagaaa ctgttgctgg agatgcattg
    12661 gcaccgtcgt tttgcgtagc tttattgttt gcaactgggc caactgatgt ttgcgtatca
    12721 ttagtttgca tatctttagc tttattgttt gcaactgagc caactgatgt ttgcatatca
    12781 ttagtatctg ctgttgccgt atcatctttt tggctaacat tagttgaagt cattttttct
    12841 tttgcttcag aagatgcaga tgttgatggt ttattcgaaa cttcagtatc agctttgctt
    12901 ggcgatttat ctgcttcgtt agatgcaacg ttagtttcag acttaagttg tcctgcatca
    12961 gtttgatttg acgtactttc ctctttatct tttgatgtat tagaaggtac atttggttct
    13021 gttatgtctg ctgaaggcaa tgtttcagtt gttgattcaa ccatactttg atttgttgaa
    13081 tctctaccat ctttttctgc cttagcttta ttttcagatt ttggttgtgc aaccttgcca
    13141 ttagttgatt gagattcagc actattattt acttcagcat tttgttttga atcatttaca
    13201 gatgcattat ctttactatc agcagatgat gctgcttctg tgctcgcagt tgttggagcc
    13261 gtcgttgttg atcctgttgg tgcattctcg tttgttgctg tagttgtact attgttattt
    13321 gttgtgcttt ctgctggcgt tgcattatct gtttctgtta caggtttatc agttgtgccg
    13381 ttattagttg attctacttc tggtttacta gttacatcgt tatccattgt cggactgttt
    13441 gttgatgcat ctgcactaga attgttatta gcttgcggtt tatcctttgc atcatcagtt
    13501 gctgatgtta ctgttgtttc acctgttgcc gcatcactat tatttggtgt tgtcggagaa
    13561 gcgtctgctt tgccattatc tgtcgtctca gatacgttag gttgtccagt gttttctggt
    13621 gttgcattag catttgaatt tgctgttgca tcattattat ctgtaccatt attagtatca
    13681 ttagcatctg gatcattctg aggcacaatc gcttcaattg caggtatcgt tacattttgt
    13741 aattcagcaa cctctgtatt tgtttgtgtt ttatctaatt tatcagcaaa tctgtcaaaa
    13801 tatctaccta aatctgtacg tgcaatttct ttcgccgatg catctgcatc tgcattttta
    13861 attatttcta tttgcttgtt aaccacttct ctaattgctt ccaaagcatt tttcttaact
    13921 actggattaa tacgttgtgc tttaagttgt tcaagcgcac tatttttgac agtagcgatt
    13981 tctgcatttg tagtttgatc agaaatatct tcagttgctt ttgataaaat gtcttctaaa
    14041 gcattcgtaa acgcttcttt ttcttcagtt gtagcatcag cgtcgacatt tacacctgct
    14101 tcaatctggt ctagtgcagt ttctaattct tcgatagctt tttgtttttc tgttgagtcg
    14161 atttgaatgt tatcaaatgc ttcaagtcct tgagctttcg ctttttcaac ttcagcagtt
    14221 gttgttgcat cagtaatacc ttgtttagct tgatctgtaa tttgtttaat cattgctaat
    14281 gcttcagttt tttcttcagc agttaactgg tcactttgat caatagattc tttcgtatct
    14341 tctgctttaa cttcaatagc ttggttcgct ttaggtttaa cagtagcatc tacttgaata
    14401 gcatcaattg ctgctttacc ttgtgttaat gcatcatcaa cgtcaccatt atccacacca
    14461 ttattaatgc tttctaatgc agtttgaaca ttttggtcaa cttgcttaat tgcttattgc
    14521 ttttcatctt gtgttgcatt agtgtcagct gaaatattat ttttcttctg atctgcataa
    14581 gcatataaat ctgttgtagc tgattctttt ttacctgttg gaattgtgta atcgttaata
    14641 ttatctaagt cattatgaat ttgaacttca atgtcatctt tagaagtagc ttgattaaca
    14701 ttttgatccg cagtttgttt taattcagca agtttttgtt tcgcttcagc aatttcactt
    14761 gaagtcgatg cgttagagtt atccgcttcg tttactttag cattatatgc atcttcaatt
    14821 ttagctaaag catctttttt gtactcacta aatgttttaa ctgcattaat tttagctttt
    14881 ccttctttaa ctgcattatc aacatattca tttgttgatg attgatcgac atttgttttc
    14941 gcttgattta attcagtatc aacttcttgt tttgcatcat taatttcttg ttgtgatgca
    15001 tttggtgttt gttctatttg tgttttctta tcagctgcag cttgatctaa ttctttttta
    15061 cctgctggtt tcttaacagg atttgcatga agttgttgaa ctttttgtac tgctgtatct
    15121 ttagcagtag ttacatcacc tgtagtagtt gctgcattaa tattatttaa accttcttca
    15181 tatgctgctc taactggtcc aatatcgtta cctttttctt cattagtagt ctcattatta
    15241 ttaagtattt cagttatttt attttgcatt tcagttagca attccgctct tgcattcgtt
    15301 ttaacatctg ttgatgcttg aattgggtcg atggcttgaa ttgcattatc ttttgcagtg
    15361 tttacgtcat caattgactg tgcattttca atattttgat taccttgtgt taattgtgcg
    15421 tctacttgtt gatttgcttg ttctttttct tcagtagtcg catctgcagt ttgtgcaata
    15481 agcgcttttt gttcgtttgc ttttgttgct aattcatctt tcgcaacatc tttaattgtt
    15541 gtatctgcag taataccttg aatatcagca actgcttgat ctttaatttg cgtaacatca
    15601 ttagttgttt gtgcatttaa gatatcttga tacgcttttt ctttagcttt taaaactaaa
    15661 tcttttgctg catttttctc ttcagttgta gcaccagttg tattatcaat tgcttgattt
    15721 tgagttgtca cagcttgatc aacatcattt ttagcatttg atttaaccga tgttgctggt
    15781 tgcgtgcttt gaattgaatt ctttccagcg tctttcgcct gatctacacc attatcatca
    15841 gttgcagctg taatattatt tttcgcgtct gtaactgctg ttgctaattg ttgaatagct
    15901 gcttcttttt cttcagttgt agcgttctga tcattattga taacatttgt ttgcgttgct
    15961 tgtaattgat caatttcatc tttagccgtt tgtttcttca caacttttgg tgttaccgca
    16021 ttaatcgctg cttctgcatt tgttttagct tcatcaactt gtgcgttagt agttgctgct
    16081 gcaatggctt gaattgcttt accattttca gtatttgctt ctgcatcagc tgcttgtttt
    16141 tcttcatctg ttgcatctgg cgtagcttga atctcttgca atttgttatt taaaattgct
    16201 gtgatttcat tacgtgcagt tgctttttta ttaactgttg gtgttacttg atcaatacta
    16261 gtttcacctg tcgttttcgc ttggtctaca tcattttgac tattagcagc ttcaatgttg
    16321 ctatttgctt gtgtcacagc attatctacg tccgcattag ccgctgcaat ttcttcagca
    16381 gtaatgtctt gcgtttgagc gattgctgtt ttacgttcat tcgctttcgt agcaattgct
    16441 tctttcgcat tatctttagt tgttgttgct ggctgaatcg cttcaatttt agcaatttct
    16501 gcatttttag ccgcttcaac ttccgcattt gtatgtgctg catctattgc tgcatcagca
    16561 gtcgtttttt cagtttggac ttgttgtttc gcagctgctt tttcttctgt tgttgaaccg
    16621 ttattagcat caattgcttt ttcttgagct tgtactttat ctgcaattgc ttgtttcgct
    16681 tgaggtttaa catttgcatc aggtgtaatg gctgcgattg tagcttcatt tgtagttttt
    16741 gcattatcta catctgtatt tgctgcagca ttatctatat cagcgtttgc agacagaaca
    16801 gcttgatcaa ctttttcttt cgctgcttgt tgttcttcag tagtcgaatc attcattgct
    16861 tcgattgctg ttttacgttc acttgctttt tgagcaattt ctgctttagc atccgatttc
    16921 ttagttgtgg cagcttgaac ttgattaatt gcagcaatac cattgtcttt agctgttgtt
    16981 acagcactgt ttgtatttgc agcatcaaga tttgttcttg cttcttgctt tttagtatct
    17041 aattctgtat atgcagcttg tttttcttct gtagttgaag cattgctatt ttgaatttct
    17101 tgtttacgtg tattatatgc gttttctact tctgtatcag ctgcaggttt aacttttgct
    17161 tgtgtttgaa ttgcattgat tttatctaat acttttgatt ttgtatcagc aacttcctgt
    17221 tttgatttaa caacttggat gtcatgtaaa ccttgctttt gagctgcttc tactgctgca
    17281 tcagcttctg ctacttcttc atttgttgca tttgaaactg tagcattttg agcttttcta
    17341 gctgttgcag cttgtttaac ttcattatag gcatccattt ttgtttgcgt agagtcagta
    17401 atattttcaa tttttgagat ttcttcgttt ttaactcttt ctaaatcttg tgcagtcgtt
    17461 gtcgcttcaa ttgctttaac accagaaact ttagctgcat taatacgttc aatagcttcc
    17521 gctacttctt catttgttgt atcaggattt aaaggtgctt gatcaatttg tgcttgaaca
    17581 acttcgtcaa attcttcaag agctgctgct ttagctgttg tagcaggtgt agtttgatta
    17641 atatcattga ttgcttttgt tttaatggct tctacttgtg catttgttgt tgctgcatca
    17701 atatctttaa ttgcctttgt ttcaatttta ccaattttat catttgctac atctttttca
    17761 tcttgtaatg atgcatttga ctttttaatt tgttgtttac gagttgtaac tgcttggata
    17821 atatcttgtt tcgcttgagg tttaactgtt ggtgtaatca catcttgttc taacactgcg
    17881 ataccattat ctttttcagt tgtgacacct tgtgatgttg tttgtttatt aatttctgcg
    17941 attgcattat ttttatcatt gttaatttta gtcacaagcg ctgcaacttc atcttgcgta
    18001 acttttttac tttgttgtgc tgctgtaacc ttttcttgag ctttagtatc taattcagca
    18061 attgctgctt gattatcaat taatgcagct tgtaatttag ttactaaacc atcaatatcc
    18121 gcttgagatg cacgatttgc agtttcaaca tgattcgcat cttcatttaa aatagtatct
    18181 gcttgttgtt taagtttatt gtattctgca attgatgctg ttgtgtaatg actattatca
    18241 acttgtgagt ttacttgttg ttgcaacgca tctttgttca tttcaaccgc tactgaaaat
    18301 ggatctgcag ttagtgtaac ttctggttgc gcattattaa ttacaacatc tgaagcagta
    18361 cgatatgtta atttttcatt aaaatcaata tttttaggtg tatcgatatt cgcaacatta
    18421 actttatatg ataattttaa agatttatct gggaataaaa cttctttagt gtgtgtacca
    18481 cgtgccgttg tcacaccttg gcttgtaaat gtaactttat ttgcagtttg atcataatta
    18541 acagtcatat ttttcaatac tgtactgtct tcattaccat tagggaatgt tgtagttaat
    18601 gaatcattaa tgtaagttac accttgtggt aatgttactt catatttaaa ttgatctgct
    18661 ttcaatgaag caccaaaatt accattgtta ccgatttcag tattaattgt aaattcttta
    18721 ttatttgttg ctttcggtgc taaatctcta tttttgacat ataaatgtga tcctgagaat
    18781 agacctacat tatcaatgaa tgaatagtat ttataaccat ctttatttgt tgtaattctt
    18841 gaagcatcag caactgctgt attgtcagga ataaattgta aatctaaacg atcagcatct
    18901 gttggtactt taaataagtg tgcagtacca gtcttagcta catttacagt tgctaattca
    18961 gtatttttct tagcatcttt aataactaaa tttgttgcac cttgcttact attcgtttgc
    19021 atagtagtaa agttgataat taattctgaa tctggtttta cattaacagt ttttgaaata
    19081 ccgttaaagt taccatgatc tgtagaatca tttgcattca cacgacctaa tgcagctaca
    19141 tttcccctag cttgatagtt ctgagggtta ttcttatcaa acatatcgct tcgtcttaat
    19201 tctgagttaa cgaaaccaat cttaccgttg ttaattaatg aataaccatt tactttatct
    19261 gtaacagtta cagttggatc ctgtctattc tcatctgttg atatggcagg atcatcaaac
    19321 gttaatgttg tattaatact gccttcacca gtattgctag catttggatc ttgagtttgt
    19381 gcgtttgctg ctacaggtgc tgctggttgc gctgctggag cattcgctgg ctgtgtttga
    19441 tttgccggtg ttgcttggtt atttccttgt cctgcttggt ttgccggtgc tgcttgattt
    19501 ccaggttgtg cttgtgcagc gttatttgga tcagcttgat taccttgtcc agctggttgt
    19561 gctgtacctg ctgccggttg tgctgcgcct cctactggtt gtgctgcgcc tcctgctgga
    19621 ttagcctgtc caccttggtt tgctggttgt gctgctggtt gtccttggtt cgcaggtgca
    19681 gctggttgtg ctgcaggatt agcttgagca ccagcatttg cgttaggctg tgcattggca
    19741 tcagctggtt gtgctggttg attttgtgca ggctgatttt gctctgctgc agacgctgtt
    19801 gtcgggttaa tagatataaa tgtaacagtg gcaattaaag ctgaaaaaat accgacatta
    19861 aattttctga tactaaattt ttgttgtctg aataaattca ttaagtcatc ctcctggttg
    19921 attattctcg ctgttaaatg atttcactta atcaactgtt aagataagta gtagcatctg
    19981 cgttaaaaac acaaagcaac tctatctaat taaaattaat tttatcatca ttatatattg
    20041 agtaccagtg tattttatat tacatattta ttactttgtt tttattttgt ttatatcatt
    20101 ttacgtttgt actataaatt atttctagaa acacaaaaaa ccgatgcata cgcatcggct
    20161 tatttgtaat acagtattta tggtatttat ttatctaatc ccattttatc ttgaaccaca
    20221 tcagctattt gttgtgcaaa tctttcagca tcttcatcag ttgctgcttc aaccatgaca
    20281 cgaactaatg gttctgttcc agaaggtctt actaaaattc gaccttctcc attcatttct
    20341 acttctactt tagtcataac ttctttaacg tcaacatttt cttcaacacg atatttatct
    20401 gttacgcgta cgttaattaa tgattgtgga tattttttca tttgtccagc taattcactt
    20461 agtgatttac cagtcatttt tattacagaa gctaattgaa taccagttaa taaaccatca
    20521 ccagttgtat tgtaatccat cataacgata tgcccagatt gttctccacc taagttataa
    20581 ttaccgcgac gcatttcttc tactacatat ctgtcgccaa ctttagtttt attagattta
    20641 attccttctt gttcaagcgc tttgtaaaaa cctaaattac tcataacagt agaaacaatc
    20701 atgtcattat tcaattcttg atttttatgc atttcttgac caataataaa cataatttgg
    20761 tcaccgtcaa cgatttgacc attctcatct actgctatga ttctgtctcc atcgccgtca
    20821 aatgctaacc caaaatcact ttcagtttca acaacttttt ctgctaattt ttcaggatgt
    20881 gtagagccac atttctcatt gatattatat ccatcaggac tacatccaat tgtttcagta
    20941 tctgcttcta aatcaccaaa taagaatggc gctaatgatg atgttgaacc atttgcacca
    21001 tctaaaacaa ttttcaaacc ttcaaagtta acatctactg ttgattttaa ataactcaaa
    21061 tatttttgtg ccccttcaaa gtaatctgaa taatgtacaa tatcattacc aactggtctt
    21121 ggtaattctg ggttttcttg atccaataat gcttcaattt cattttcttg ttcatctgat
    21181 agtttaaaac catctgatcc aaagaattta ataccattat ctgcaactgg attatgagag
    21241 gctgaaatca ttacacctaa ctctgcaccc atatcgcgtg ttaaatatgc aacacctggt
    21301 gttgaaataa tacctaatcg catcacttct gcaccaattg aaatcaaacc agctattaat
    21361 gctgattcta acatttcacc tgaaactcta gtatcgcgac ctacaagtac acgtgggtgt
    21421 ttttcacctt tattatgtgc tagaacatag ccaccgtatc ttcctaattt aaatgccaat
    21481 tcaggtgtta gttcttggtt tgcgacacct cttactccgt ctgtaccaaa atattttccc
    21541 attgtttact ctcctttaat ttagctattt attttacatt tatataagcc ttcgtttcac
    21601 ttggttgtgc tttagtgaca tgttctggta aattgatttt tacagtcttt tcagttgatt
    21661 ctgaaatacc atctaaatct acttctgcat caacttcgct tatattttgt aagtcatctc
    21721 gactaccgaa gatttcaatt tctttatctt ctaaatcaat cgaacttaac tctttatcat
    21781 ctgctaaact acctttctgt ttaacattta ctttaacctt tttgctaaaa ggctctactt
    21841 taacttgtaa attcacttca ttaggttgaa tcgatacatt taatttattc agttttttat
    21901 caaaagccgt tacttctgcg acatcttttg tgtcaccatt aatcttttta ttagttttaa
    21961 aagtggcttt taaataagcg atatcattca attgttcttc tccacctgtt actttaactg
    22021 tttgtggtga aacttcttgc tttgtaattt tataaagtgg atcaatatca ctctgactta
    22081 catcaggttg aactgtcatc tttttagtta ctttgttttc aagcgtaata tttgctaatt
    22141 taggttttac agaataatga atgtcatcag ctaacccttt aacttgatac ttctcttgat
    22201 attttccagc tttagcattt gataaatcaa tcactactct taaatcttct ggattttcaa
    22261 tttttattat ctttgattgt ggtcctgaaa tagtcacatt aactgtttca ggcgcttttg
    22321 ttaaatgcaa atctttagtg ttataaagaa tttcaacggg tacatcttga atcgttttac
    22381 tagacttttg accaagatta ccagtgttaa agatatttcc aaaaacattg ttaacagata
    22441 aaaagaaaaa caatgccaaa agaaaggcaa taaatctcaa gccccattta ctttctagca
    22501 tattatttca cacctttctt ttgaaagcgt gtgccaaacc aatgttcagc aagcaactct
    22561 tcaaaaattt cgtttgaaat gtctcgtcgt aattttccat caaatgttac cgaaatatca
    22621 ccagtttctt cagatacaat aacggtaaat gcatcagata cttctgaaat accaaccgca
    22681 gctctatgtc ttgtacccaa acttttagat atcttaggac tatcagacaa tggcaaataa
    22741 cttgctgctg ctgcaatctt cgtgccttga ataatcattg caccatcatg taaaggtgtg
    22801 ttaggtataa agacattaat taaaagttct tgcgaaatat ttgaatccat tgcaatacct
    22861 gtttcaatat aatcttgaag acctgtttct ttttcaaaga caattaatgc acctatacgt
    22921 cttttagcca tatattgcac agcctttgaa accgattgaa tcaatttctc ttcatcttta
    22981 ctatacgtat tagaagtata gcgttttaaa aagctacctc taccaagttg ttctaacgca
    23041 cgtctaattt ctggttggaa tattactatt aaagctaata ccccccattg aataacgata
    23101 tcgaataatt tagatgttgc agtcaagttc aatatcatac ttatctgctg accaataaca
    23161 attactaata tccctttaag taattgtatc gctttagttc ccttaaagac cgtgatgaga
    23221 agataaagta cataccaaac tatcagtaaa tcaaggatac tcgttacaat ttttaacgta
    23281 ctgaggtttt gaaaaaagtt ggaaaaatcc ataacatctc ctccgggtaa tatttttcca
    23341 taatacccat tataccaatc atttacatca ctgtcatacg aacatatatt taaatagaaa
    23401 aaatttattt taaagattat aactactctt aatcatttta gtgaattaaa aaaagtagtg
    23461 caaaaagcaa aatatacttt atacactaca aatcatttat ttataataaa gtttcaccaa
    23521 aaaatgttcc aactaatgaa accgcttgtt cagcagtatg attattactg tcaatcaatg
    23581 gatttacttc aactaaatcc attgaggaaa ttaaatgtga ttgatgcagt aattccaatg
    23641 caaaatggct ttctctataa ctaagaccac ccaaaactct agtaccagtg cctggcgttt
    23701 caagcggatc taaagcatca acatctaaag ataaatgaac gccatcaaca ttgcgtgact
    23761 tcaaatattc tattgtattt tcaattactt cctttatccc caatttatca atatctgaca
    23821 tagtaaatgt tttaatatta tgatctttga taaattgtct ttcaccttta tctaaatctc
    23881 tcataccaat tagtacgatg ttttctggct tgattacatt actatttaat tctaaaagtt
    23941 ctttgggacc ttcgcctgtc aaaatcctta gaggcatacc atgaatattt ccacttggtg
    24001 actcttcagg tatatttaaa tcaccatgtg catcatacca aataacacct aaattattat
    24061 aatgtttact tattgctgat actgaaccta ccgcaataga atgatctcca ccaagaacta
    24121 gaggaaatct gttattttca attgatgctg aaacctcttt atttaatttt tgattaacat
    24181 ctataatttc atcataattt cttaatcctt tttgttcact atgaaatttt tcaatgttca
    24241 caacaggtac cttaatatcc cccttgtcat atacatcaag gtctaattgc tttaatcttg
    24301 aaattaatcc agcatatcta attgctgttg gtcctaaatc aacacctaat tttctttgtc
    24361 caaatgttga tggtgcacct ataatatcaa ttgcttttgt ctttgtcata agcgtccccc
    24421 tttgctctat gtaattaaag aataatgtat gcgcttacca ttatcaagca atagctacac
    24481 atataatctg tttatcttat tacttcataa aaaaaagcac ttcccaaaaa tggaaagtgc
    24541 aagtagtgag ccatagagga ttcgaacctc tgaccctctg attaaaagtc agatgctcta
    24601 ccaactgagc taatggctct aatggctggg ctagctggat tcgaaccaac gagtgacgga
    24661 gtcaaagtcc gttgccttac cgcttggcta tagcccaata tatagatggt ggaggggggc
    24721 agattcgaac tgccgaaccc gaaggagcgg atttacagtc cgccgcgttt agccacttcg
    24781 ctacccctcc agcttattca tataatttaa taatcaaaat ggtggagaat gacgggttcg
    24841 aaccgccgac cctctgcttg taaggcagat gctctcccag ctgagctaat tctccaaaat
    24901 aatgactcct acgggactcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc
    24961 ttgaccaagg agccatggct ccacaggtag gactcgaacc tacgaccgat cggttaacag
    25021 ccgatagctc taccactgag ctactgtgga ttaatatt
//
LOCUS       DAMZLE010000026        23856 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMZLE010000026 DAMZLE010000000
VERSION     DAMZLE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.026"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            135..599
                     /gene="accB"
                     /locus_tag="POB92_000938"
     CDS             135..599
                     /gene="accB"
                     /locus_tag="POB92_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI6578448.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQVQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            599..1954
                     /gene="accC"
                     /locus_tag="POB92_000939"
     CDS             599..1954
                     /gene="accC"
                     /locus_tag="POB92_000939"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6578449.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            1969..2331
                     /locus_tag="POB92_000940"
     CDS             1969..2331
                     /locus_tag="POB92_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6578450.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2391..2780
                     /gene="nusB"
                     /locus_tag="POB92_000941"
     CDS             2391..2780
                     /gene="nusB"
                     /locus_tag="POB92_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI6578451.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2797..4134
                     /locus_tag="POB92_000942"
     CDS             2797..4134
                     /locus_tag="POB92_000942"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI6578452.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4127..4357
                     /locus_tag="POB92_000943"
     CDS             4127..4357
                     /locus_tag="POB92_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI6578453.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4335..5216
                     /locus_tag="POB92_000944"
     CDS             4335..5216
                     /locus_tag="POB92_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI6578454.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            5648..6100
                     /gene="argR"
                     /locus_tag="POB92_000945"
     CDS             5648..6100
                     /gene="argR"
                     /locus_tag="POB92_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDI6578455.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            6116..7795
                     /gene="recN"
                     /locus_tag="POB92_000946"
     CDS             6116..7795
                     /gene="recN"
                     /locus_tag="POB92_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI6578456.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            7946..9367
                     /gene="lpdA"
                     /locus_tag="POB92_000947"
     CDS             7946..9367
                     /gene="lpdA"
                     /locus_tag="POB92_000947"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6578457.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            9383..10375
                     /locus_tag="POB92_000948"
     CDS             9383..10375
                     /locus_tag="POB92_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDI6578458.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            10375..11358
                     /locus_tag="POB92_000949"
     CDS             10375..11358
                     /locus_tag="POB92_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6578459.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            11371..12645
                     /locus_tag="POB92_000950"
     CDS             11371..12645
                     /locus_tag="POB92_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDI6578460.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTFTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            12996..13433
                     /locus_tag="POB92_000951"
     CDS             12996..13433
                     /locus_tag="POB92_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDI6578461.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            13447..14427
                     /locus_tag="POB92_000952"
     CDS             13447..14427
                     /locus_tag="POB92_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6578462.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(14554..14733)
                     /gene="prli42"
                     /locus_tag="POB92_000953"
     CDS             complement(14554..14733)
                     /gene="prli42"
                     /locus_tag="POB92_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDI6578463.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            14996..16129
                     /locus_tag="POB92_000954"
     CDS             14996..16129
                     /locus_tag="POB92_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDI6578464.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            16197..17603
                     /gene="gndA"
                     /locus_tag="POB92_000955"
     CDS             16197..17603
                     /gene="gndA"
                     /locus_tag="POB92_000955"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI6578465.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            17822..18193
                     /locus_tag="POB92_000956"
     CDS             17822..18193
                     /locus_tag="POB92_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6578466.1"
                     /translation="MEKLNQVMLYVDDVDKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            18132..18386
                     /locus_tag="POB92_000957"
     CDS             18132..18386
                     /locus_tag="POB92_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578467.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTYILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            18387..19406
                     /locus_tag="POB92_000958"
     CDS             18387..19406
                     /locus_tag="POB92_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6578468.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETVASQFNLDYQI
                     IETSNERKVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            19422..21071
                     /locus_tag="POB92_000959"
     CDS             19422..21071
                     /locus_tag="POB92_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI6578469.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(21153..22019)
                     /locus_tag="POB92_000960"
     CDS             complement(21153..22019)
                     /locus_tag="POB92_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6578470.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERTPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(22249..23733)
                     /locus_tag="POB92_000961"
     CDS             complement(22249..23733)
                     /locus_tag="POB92_000961"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI6578471.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         8670 a         3351 c         4476 g         7359 t
ORIGIN      
        1 atttaaaatg tgtttttatt atatctttgg gatagactta taatgtcgcc agcaatatga
       61 acatgcttga attgaagagt tgtctcaagt aaaatagacg ggtagatgaa aacaaactga
      121 aggagtcagt aataatgaac tttaaagaaa tcaaagaatt aattgaaatt ctggataaat
      181 caactttaac ggaaatcaat attgaagata ctaaaggcaa agtgacgctt aagaaagaaa
      241 aagaaactga gattatcacg ccacaaatct cacaaatgcc agttgaagct gcggcaatgc
      301 ctatgcctca agtacaatca actgatagca ataaaactga agctccaaag ccaacttcag
      361 ataatcacaa aacaattaat gcacctatgg taggtacatt ttacaaatcg ccatctccag
      421 acgaagaagc atatgtgcaa gttggggaca ctgtttcaaa tgaaacaaca gtgtgtattt
      481 tagaggcaat gaaactattt aatgaaattc aagcagaaat ttcaggtgaa attgttgaaa
      541 tcttagtaga agacggacaa atggtagagt atggccaacc gttatttaag gtgaaataat
      601 gaaaaaggtt ttaattgcaa accgcggtga aatcgcagtt aggattattc gcgcttgtcg
      661 tgatttaggc atccaaactg ttgcaatcta ttctgaaggg gataaagatg cgctacatac
      721 tcaaattgct gatgaagcat attgcgtagg tcccactttg tctaaagatt catatttaaa
      781 tattccgaac atcttatcta ttgcaacttc tacaggttgt gatggcgttc atccgggtta
      841 tggcttttta gctgaaaatg ctgactttgc agaattatgc gaagcatgcc aattgaagtt
      901 cattggacca agttatcaat ctatccaaaa aatgggtatc aaagatgttg ctaaggcaga
      961 aatgatcaaa gccaatgttc cagttgttcc tggtagtgat ggtttaatga aagacgtctc
     1021 agaagctaag aaaatcgcta aaaaaattgg ctatccggtc atcattaaag ctactgctgg
     1081 tggtggcgga aaaggtattc gtgttgctcg tgatgaaaaa gaacttgaaa ctggcttccg
     1141 aatgacagaa caagaagctc aaactgcatt tggtaatggt ggactttata tggagaaatt
     1201 catcgaaaac ttccgccata ttgaaatcca aattgttggg gacagctatg gtaatgtaat
     1261 tcatttagga gaacgtgatt gtacaattca aagacgtatg cagaaattag tggaagaagc
     1321 accttcccca attttagatg atgaaacacg tcgtgaaatg ggaaatgccg cagtgcgtgc
     1381 agcgaaagct gtaaattatg aaaatgcggg aacaattgag tttatatatg atttaaatga
     1441 taataaattt tattttatgg aaatgaatac acgtattcaa gtagaacatc ctgtaactga
     1501 aatggtaaca ggaattgatt tagttaaatt acaattacag gttgctatgg gtgacgtgtt
     1561 accgtataaa caagaagata ttaaattaac aggacacgca attgaattta gaattaatgc
     1621 tgaaaatcct tacaagaact ttatgccatc accaggtaaa attgagcaat atcttgcacc
     1681 aggtggatat ggtgttcgaa tagagtcagc atgttatact aattatacga taccgccata
     1741 ttatgattcg atggtagcga aattaatcat acatgaaccg acacgagatg aagcgattat
     1801 ggctggcatt cgtgcattaa gtgaatttgt ggttcttggt attgatacaa ctattccatt
     1861 ccatattaaa ttattgaata acgatatatt tagaagcggt aaatttaata caaacttttt
     1921 agagcaaaat agcattatga atgatgaagg ttaataggag gtcaatccat ggtcaaagta
     1981 actgattatt caaattcaaa attaggtaaa gtagaaatag cgccagaagt gctatctgtt
     2041 attgcaagta tagctacttc ggaagtcgaa ggcatcactg gccattttgc tgaattaaaa
     2101 gaaacaaatt tagaaaaagt tagtcgtaaa aatttaagcc gtgatttaaa aatcgagagt
     2161 aaagaagatg gcatatatat agatgtatat tgtgcattaa aacatggtgt taatatttca
     2221 aaaactgcaa acaaaattca aacgtcaatt tttaattcaa tttctaatat gacagcgata
     2281 gaacctaagc aaattaatat tcacattaca caaatcgtta ttgaaaagta atgtcatacc
     2341 taattcagta attaaataaa gaaaaataca aacgtttgaa ggagttaaaa atgagtcgta
     2401 aagaatcccg agtgcaagct tttcaaactt tatttcaatt agaaatgaag gacagtgatt
     2461 taacgataaa tgaagcgata agctttatta aagacgataa tccagattta gacttcgaat
     2521 ttattcattg gctagtttct ggcgttaaag atcacgaacc tgtattagac gagacaatta
     2581 gtccttattt aaaagattgg actattgcac gtttattaaa aacggatcgt attattttaa
     2641 gaatggcaac atatgaaata ttacacagtg atacacctgc taaagtcgta atgaatgaag
     2701 cagttgaatt aacaaaacaa tttagtgatg atgatcatta taaatttata aatggtgtat
     2761 tgagtaatat aaaaaaataa aattgagtga tgttatatgt cagattattt aagtgtttca
     2821 gctttaacga aatatattaa atataaattt gatcaagatc cacatctcca atctgtattg
     2881 attaaaggtg agctttcaaa tttcaaaaag cattcaagtg gtcatttata ctttaatgtg
     2941 aaagataaag aaagcgttat aagtgccatg atgttcaaag gtagtgcttc taaattaaac
     3001 ttcgaaccta aagaaggaga tgaagtctta ttagaagcac gtgtttctgt ttttgaacgt
     3061 cgtggaaact atcaaattta tgtaaataaa atgcaattag atggtatagg gaacttatat
     3121 caaaaattag aagcattaaa gaaaaaatta actgaagaag gttgttttga taaagcgaat
     3181 aagaaatcaa tacctaagtt tcctaaaaaa attgcagttt taacagcgag tacaggtgcc
     3241 gcaattcgag atatccattc aacgataaat agtcgttttc cattagctga acaaatacaa
     3301 attagtactt tggttcaagg tgaaaaagca aaggacgaca ttattgaaaa aattgaatat
     3361 gcagatagtt taggtgtaga taccattatt gtaggtcgag gcggtggatc catagaagat
     3421 ttatggaatt ttaacgaaga agctgtcgtc cgtgcaatat ataattgtaa gacaccgatt
     3481 atatcagcag taggtcatga aacagacttt acattaagtg attttgctgc agacatcaga
     3541 gctgcgactc caactcaagc tgctgttatt gcaacaccag accaatatga attgctgcaa
     3601 caaatacagc aatatcagtt tacattgact cgtttcatca agaaacattt agaacaacaa
     3661 cgtaaacatg ttgaacattt gtcatcgtat tacaagttta aacaaccaac tttattgtat
     3721 gatcaacaga tacaacgtcg agatgattta gaaaagagac tgaaacaaca aattcaggca
     3781 acttttgaac aacagagaca tcgattaatg ctgttgcaac aacgttataa tttaaaagca
     3841 ttactaagta gtgttaatca agaacaacaa aataatcttc aattgacaaa ccaacttgtt
     3901 aaattattga attctaagat attaagttat aaaaatgatt tgaaaaataa agtagaaaac
     3961 ttaaacaatt taagtccaac taatacaatg ttgcgtggat atgcaattgt taataaaaaa
     4021 gacgaagtca ttacgagtac taaagattta acagaaaatg atcaattgac gttaacaatg
     4081 aaagatggct tagtagatgc aaaagttacg aaagtaaggt gtaataatga ctaaagaaac
     4141 gcaaagtttt gaagaaatga tgcaagaatt agagcaaatt gttcaaaaat tagataatga
     4201 aacagtatct ttagaggaat cattagattt atatcaacgt ggtatgaaac tatcagcagc
     4261 ttgtgacaca actttaaaaa atgccgaaaa aaaggtgaat gacttaataa aagaagaagc
     4321 tgaggatgta aaaaatgacg aatctaccga tgaataaatt aatagatgaa gtcaataatg
     4381 aattatcggt tgcgataaat aaatcagtaa tggatactca gctagaagaa agtatgttgt
     4441 attcattaaa tgctggaggt aaacgcatcc gaccagttct gttattactc actttagatt
     4501 cactaaatac cgagtatgag ttaggtatga agagcgcaat tgcactagaa atgattcata
     4561 catattcact tattcatgat gacctaccag cgatggataa tgatgattat cgacgaggaa
     4621 aattaacaaa tcataaagta tatggtgagt ggactgcgat attagcaggt gatgctttat
     4681 taactaaagc atttgaactt atttcaagtg atgatagatt aactgatgaa gtaaaaataa
     4741 aagttctaca acggctgtca atagcaagtg gtcatgttgg aatggtcggc ggtcaaatgt
     4801 tagatatgca aagcgaaggc caaccaattg atcttgaaac tttggaaatg atacacaaaa
     4861 caaaaacagg agcattatta acttttgcgg ttatgagtgc agcagatatc gctaatgtcg
     4921 atgatgcaac taaagaacat ttagaaagtt atagttatca tttaggtatg atgttccaga
     4981 ttaaagatga tttattagac tgctatggtg atgaagcaaa gttaggtaaa aaagtgggca
     5041 gcgatcttga aaataataaa agtacgtacg tgagtttatt agggaaagat ggcgcagaag
     5101 ataaattgac ttatcataga gacgcagcag tggatgaact aacgcaaatt gatgaacaat
     5161 tcaatacaaa acacttatta gaaatcgttg atttatttta tagcagggat cactaaaagc
     5221 aaatatatcg attagcaaca attgtaaagt ttgaaatgtc tcaaaaggca tattcaactg
     5281 acgttgtttg ttaatcgatt tttctcatgt tgaaacttaa aattatgttg aagactttca
     5341 tttaaaaaat agaattttca atttctatac gacaatattc gtatctgtga tatcaatgtg
     5401 attttaatta aatatgttca aacaattgac gcttattaaa tctaataatt agcaagttta
     5461 ttacacatat attcattttt atacatattt taagcaattg ttatgcgtaa tatgaaaaat
     5521 atttataaat agataaccaa gatagataaa ccatgtttac cctttttacg taaaggtgtt
     5581 tagtagtcgt aaatcagtgc atgtgttaaa ataaatgtat aaatattcgt aatcagaggt
     5641 gtttactgtg cccaaaaaat cggttaggca tataaaaatt agagaaatta tttcaaatga
     5701 acagatagag acacaagatg aattagttaa acgattaaac gattatgatt taaatgtcac
     5761 tcaagcaact gtttctcgtg atattaaaga actacaactt attaaagtac ctataccttc
     5821 aggtcaatat gtttatagtt taccaaatga tagaaaattc catcctttag aaaaattggg
     5881 acgttattta atggattcct ttgttaatat agatggtact gataatttac ttgttctaaa
     5941 aacgttacct ggtaatgcac aatctattgg agctatatta gaccaaatca attgggaaga
     6001 agtactaggc acaatttgtg gtgatgatac ttgtttaatt atttgtcgaa gcaaagaggc
     6061 aagtgatgaa atcaagtcaa gaattttcaa tttgttataa ggatgcgatg agtttatgtt
     6121 acaaacctta tcaatcaagc aatttgctat tattgaagag ttagaaattc aattttctga
     6181 tggtttaact gttttaagtg gtgaaacggg ttcaggtaag tcaattatta tagatgcaat
     6241 tggacaatta attggaatga gagcttcatc tgattttgta agacatggtg aaaaaaaggc
     6301 tgtcatcgaa ggtatatttg atattgacga gagtaaagat gctatacata tattgaaaaa
     6361 tatggatatt gatgtagatg aagatttttt attagttaaa cgggaaattt tcagctctgg
     6421 taaaagtctt tgtaaaatta ataatcaaac agttacttta caagatttac gaaaagttat
     6481 gcaagaatta ttagatattc atggccaaca tgaaacgcag tctttgttga aacaaaaata
     6541 ccatttaaca ttattggata attacgcaga atcacgttat caagatttgt tggataaata
     6601 tcatcaaact tttcaaaatt ataaagccaa aaagcaagag ttagaagatt tagaatcagc
     6661 agaccaagca ttgctgcagc gtttagattt aatgaaattc caattagaag aattgtctga
     6721 agcacatcta aaagaaggcg aaatagagca acttgaaata gatattaaac gcattcaaaa
     6781 ctctgaaaaa ttaagcttag cgcttaataa cgcacatatg acattaacgg atgaaaatgc
     6841 tatcactgat cgtttgtatg aactaagcaa ccatttgtta acaataaacg atatagttcc
     6901 aaacaaatat gacaaattaa aagaagatat tgatcaattt tactacattt tagaagatgc
     6961 aaaacatgag ttatatgatg aaatggctaa tacagaattc gacgaacaag tgttaaacga
     7021 gtatgaatct cgtatgaatt tgctgaataa tttaaaacgt aaatatggaa aagatatttc
     7081 agaattaatc gcatatcaag aaaagcttaa taatgaaatc aataaaattg aaaactatga
     7141 acaaagtaca tcgcagttac gagaagaaat aaatgcattg tataatcaag ttatagaggt
     7201 tggacaagcg ttatcaaagc agcgtcgtat agtcgctagg gagttaagag accacattgt
     7261 atcggaaatt caaaacttac aaatgaaaga cgcaaatctt gagatttcat ttaaaaaatt
     7321 agaagaaccg aatattgatg gaatcgaatt tgtagaattt ttaatcagtc caaataaagg
     7381 ggaaccatta aaaagtttaa ataaaattgc gtcaggtgga gaactttcta gaattatgtt
     7441 agcgttaaaa agtatttttg ttaaatcgag aggtcaaact gcaattttat tcgatgaggt
     7501 tgactcaggt gtatctggac aagctgcaca aaaaatggca gagaaaatgc gagatatagc
     7561 tgaatatata caagttattt gtatctctca cttaccacaa gttgcatcga tgagcgacca
     7621 tcatttatta atttcgaaat catcaaaaga tgatcgtaca acaacacaag ttcaagagtt
     7681 aattagcgat gataaagtag atgaaattgc acgaatgatt tcaggtgcaa gtgttactga
     7741 tttaactcga gaaaatgcaa gagaaatgat acaacacaat caaagacgta gataatttcc
     7801 aaagtgtgtt gaatactcat ttatggattt atttatgtgc attcaaacta aatagacaat
     7861 catgtaaaat aggtaattaa tggacatgaa aactatgtat taataaattt tcaatggata
     7921 tatatcatta gttaggagta agaacatgtc agagaaacaa tatgatttag tcgttctcgg
     7981 tggaggtact gcaggatatg ttgctgcgat acgtgcgtct caattaggta aaaaagtggc
     8041 aatagtagaa cgacaactat taggaggtac ttgtctccat aaaggttgta ttcctactaa
     8101 atcgctgctt aaatctgctg aagtatttca aacagtaaaa caggcagcaa tgtttggcgt
     8161 tgatgtcaaa gatgctaatg ttaattttga aaatatgttg gcacgaaaag aagacattat
     8221 taatcaaatg tatcaaggtg taaagcattt aatgcaacac aatcacattg acatttataa
     8281 tggcacggga cgtattctag gtacatctat attttcacct caaagtggca caatttctgt
     8341 cgaatatgaa gatggtgaat cagacttatt accaaaccaa tttgtattaa ttgcaactgg
     8401 ttcttcgcct gcagagttgc cattcttatc atttgatcat gataaaattt tatctagcga
     8461 tgatatatta tcattaaaga cgttaccatc aagtattggt attattggtg gtggtgttat
     8521 cggaatggaa tttgcatcat tgatgataga tttaggtgtt gacgtaacag ttatagaagc
     8581 aggtgaaaga attttaccaa ctgaaagtaa acaagcttca caactattaa aaaaatcatt
     8641 gtcagcacgt ggtgttaaat tctatgaggg aataaaactt tctgaaaatg atataaatgt
     8701 taacgaagat ggtgttacgt ttgaaatttc ctcagacata attaaagtag ataaagtgtt
     8761 gctatcgatt ggtagaaaac ctaacacatc agatataggt ttaaacaaca ctaaaataaa
     8821 actttcaaca tcaggacata ttttaacgaa cgaatttcaa caaactgaag ataaacatat
     8881 ttatgcagct ggtgattgta ttggaaaatt acaattggca catgttggat caaaagaagg
     8941 tgttgtggct gttgatcata tgtttgaggg gaacccaatc ccagtaaact ataacatgat
     9001 gcctaagtgt atttattcac aacctgaaat tgcttctatc ggtttaaaca ttgaacaagc
     9061 aaaggcagag ggaatgaaag ttaaaagttt taaagtacca tttaaagcaa ttggtaaagc
     9121 agtgattgat agccatgaca caaacgaagg gtatagcgaa atggtgattg atcaatcaac
     9181 tgaagaaatt gtgggtatta atatgattgg tccacatgta acagaattga ttaatgaggc
     9241 atcactgttg cagttcatga atggctcggc attagaatta ggactaacaa cacacgcaca
     9301 tccttccatc tctgaagtgt tgatggaatt aggattgaaa gcagaaagta gagctattca
     9361 cgtataaata ggaggattta acatgattga ttataaatca ttaggcctta gcgaagaaga
     9421 cctaaaagta atatataaat ggatggattt aggtagaaaa atagatgaaa gactgtggtt
     9481 attgaaccgt gcaggtaaaa ttccatttgt tgtaagtggt caaggacaag aagcaactca
     9541 gattgggatg gcatacgcat tagaagaagg tgatattaca gcgccttatt atagagatct
     9601 agctttcgtt acttatatgg gtatctcagc gtatgatacg tttttatcag cgtttggaaa
     9661 gaaagacgat gttaactctg gtggaaaaca aatgccatca cactttagta gtagagctaa
     9721 gaatattttg tctcaaagtt caccggtagc aactcaaata ccccatgctg ttggtgctgc
     9781 tttagcttta aaaatggatg gtaagaagaa aattgcaaca gcaactgttg gtgaaggtag
     9841 ctcaaaccaa ggtgacttcc atgaaggttt aaacttcgcg ggtgtacaca aacttccttt
     9901 tgtatgtgtc attataaata ataaatatgc aatttcagta cctgattcat tacaatacgc
     9961 agctgaaaaa ttatcggata gagcacttgg ttacggtata catggtgaac aagtagatgg
    10021 taatgatcca ttagcaatgt ataaagcgat gaaagaagca agagatcgag ctatttctgg
    10081 tcaaggttca actttgattg aagcagtaac aagtcgtatg acagctcatt catcagatga
    10141 tgacgaccaa tatcgtacga aagaagagcg tgaagcgctt aaaaaagctg actgcaatga
    10201 aaagttcaaa aaagagttgc tttcagctgg cattatcgat gatgcttggt tggcagaaat
    10261 agaagcggag cataaagaca tcatcaataa agcgacaaag gctgctgaag acgcgccata
    10321 tccaagtgtc gaagaagcct atgcatttgt gtacgaagaa gggagtctta actaatggct
    10381 aaattatctt atttagaggc gatacgccag gcacaagact tagcattaca acaaaataaa
    10441 gatgtgttta ttcttggtga agatgttggt aaaaaaggtg gcgtatttgg tactactcaa
    10501 ggactacaac aacaatatgg tgaagacaga gttatcgata cgccattagc tgagtcaaat
    10561 attgttggaa ctgccattgg tgcagctatg gtaggtaaaa gaccaattgc agaaattcaa
    10621 tttgcagatt ttattttacc tgcgacaaat caaattatta gtgaagctgc gaaaatgcgt
    10681 tatcgttcaa ataatgattg gcaatgccca ttaacgattc gtgcaccatt tggtggcggt
    10741 gttcacggag gactatatca ttcacaaagt attgaaagta tatttgcttc atcaccaggt
    10801 ttaacaattg ttattccttc cacaccgtat gatgctaaag ggcttttatt atcatctatc
    10861 gaatcaaatg atccagtatt gtactttgag cataaaaaag cttatcgttt cttaaaggaa
    10921 gaagtgccag aagaatatta cactgtacct ttaggaaaag cagatgttaa acgtgaaggt
    10981 gaagatttaa cagtattttg ttatggatta atggtcaatt attgtttaca agcagcggat
    11041 attttagctg cagacggtat caacgttgaa gtcgttgatt tacgaactgt ttatccatta
    11101 gataaagaaa caattattga tcgtgctaaa aatacaggta aagttttact tgtaactgaa
    11161 gataatttag aaggtagtat tatgtcagaa gtgtcagcga ttattgcaga gcattgcttg
    11221 ttcgatttag atgcaccaat catgcgttta gctgctccag atgtaccatc tatgccattt
    11281 tctcctgtat tagaaaatga aattatgatg aatccagaaa aaatcttaaa taaaatgcgt
    11341 gaattagcag aattctaggg agggaaagtc atggaaataa caatgcctaa gttaggtgag
    11401 agtgttcatg aaggtaccat tgaacaatgg ttagtttctg ttggtgatca tattgatgaa
    11461 tatgaaccat tatgtgaagt tattacagat aaagtgacag ctgaagtccc ttccacgata
    11521 tcaggaacaa ttacagaaat tttagttgaa gcggggcaga cagtagctat tgatacaatt
    11581 atctgtaaaa ttgaaactgc tgatgaaaag acaaatgaaa caactgaaga gatacaagca
    11641 aaagtggatg agcatactca gaaatctact aaaaaagcta gtgcaacagt ggaacagaca
    11701 tttactgcta aacaaaatca acctcgtaat aatggtcgct tttcacctgt tgtatttaaa
    11761 ctcgcttcag agcatgacat tgatttatca caagttgtag gtagtggatt tgaaggtcgt
    11821 gtaactaaga aggatataat gtcagttatt gaaaatggtg gtaccacagc tcaatctgac
    11881 aaacaagttc aaacaaaatc aacatcagta gatacatcaa gtaaccaatc atctgaagac
    11941 aatagtgaaa acagcacaat accagtaaat ggtgtgcgta aagcaattgc gcaaaatatg
    12001 gttaatagtg taacagagat tccacatgca tggatgatga ttgaagtaga tgctacaaat
    12061 cttgtgaata cgagaaatca ttataaaaac agctttaaaa ataaagaagg atataatcta
    12121 acgttctttg ctttctttgt aaaagctgta gcagatgctt taaaagcata tcctttatta
    12181 aatagtagct ggcaaggaaa tgaaattgtc ttacataaag acattaatat ttcaattgct
    12241 gttgctgatg aaaataaatt atacgtacct gtgattaagc atgcagacga aaagtcaatc
    12301 aaaggtatag ctagagaaat taatacttta gcaacgaaag cgcgtaataa gcaattgaca
    12361 gctgaagata tgcagggcgg tacatttacg gtaaataata ctggtacatt tggttcagta
    12421 tcatcaatgg gtattataaa tcatccacaa gcagcgattt tacaagtaga atcaatcgtt
    12481 aaaaagccag tagtaattaa tgatatgatt gcaattcgta acatggttaa tttatgtatt
    12541 tcaattgatc atcgtatttt agatggttta caaacaggta aatttatgaa tcatattaaa
    12601 cagcgtatcg aacagtatac tttagaaaat acaaatatat attagtgata acatagatgc
    12661 atctatcgac aacttgtttt atcttgttct tgtcgatgga tgtattattt tttggcacta
    12721 aaatatgtgc aatatattca aaaaagataa agaacaataa tcaacatggt tgaatgcatt
    12781 tttgcagtaa agtcaaaata agacatcata cttgaaacat attaatgaaa acatgtgaac
    12841 aaattagtta ccatgatttt aagcacaata atgtttggta tattgttaaa attgtgtcta
    12901 aatataggtg tgattcagat tagtttattg aacaatatgt tattaattag tagaatgagg
    12961 atagtttaaa tataaaggga taggtgattg aacttatgga catgaatttc gatttataca
    13021 tgaacggtgt tgtagaacaa gcaaggaatg aaattgaatc tgcgggatat gagcaattaa
    13081 ctactgcaga agatgttgac aaagttctta aacaagatgg tacaacacta gttatgatca
    13141 attctgtatg tggttgtgca ggtggtatcg caagaccagc agcatcacat gctttacatt
    13201 atgacgtatt acctgatcgt ctagtgacag tatttgctgg acaagataaa gaagcgacac
    13261 aaagagcgcg tgaatacttc gaaggttatg cgccttcaag tccgtcattt gcattagtaa
    13321 aagatggaaa gattacagaa atgattgaaa gacatcaaat cgaaggtcat gatgtgatga
    13381 acgtaattaa tcaattacaa acattattca ataaatattg tgaagaaaga taagaggcgc
    13441 taacccatgt taaagttaaa tccttacaag attggattta gaacaataaa aacagcagtg
    13501 ggtatgactt taggtgtaat tattagtaag ctgttaggtt tagataatta tgcttcaagc
    13561 gccatattag tcgtattatg tattaaacat acaaaagtac attcgctaca agcgattatt
    13621 tcaagattag tatcatgttt tttagtattg tttttaggtt cagcaatatt tagtttatta
    13681 ggtcagagtc caattgtact cggtattatc gtattgttat ttataccatt aactgtcgta
    13741 ttaaaagtac aagaaggtgt cattacgagt tgcgttatat tacttcatgt ttttaatgca
    13801 aaatcaattg atgcacattt aattgttaat gaaacattat tactgttaat tggactgagc
    13861 attgcattta caatgaattt aatgatgcca agtttagaca aacaactaga cgaatacaaa
    13921 tgtaaaattg agcaacaaat tgctgatatt tttagtaaat atagttatat ttgtgaaaag
    13981 tatgaagata ccattgcgat tgaatttgaa gtgttacttt taaatattaa aaaggcgaag
    14041 tctatcgcgt tccgagatgt taaaaatcat tttgttagaa acgaaaattc atactatcat
    14101 tattttgata tgcgagaaga gcaagtggaa ttgttaatga gaatgaaacc gctcatcgaa
    14161 agtatctgtc ataaagatcc acttttaaat gatgttgcgt cactgctgaa agaaattggt
    14221 ctaaatgtga atagtaatga ctatacagca atgcgtttac acaatttgta tgagacgcgt
    14281 ttgaaattgg atgaacttcc attgccaaca acacacaaag cgcttaactc gagagcaagt
    14341 attattcaaa tattaaatga attagaagaa tatttacaaa tcaaatcgca atttggttca
    14401 ttgaaaatgc atagtgaagt agcttaaaaa ataatccatc cgtatgaccc atgtttgaaa
    14461 ataatcgttg caatacgata acttattaat acatgcgttt ataacggatg gatttttaat
    14521 tgtatgattt tgtataggta gttgagatat agtttataaa attggaatga tactcgttaa
    14581 taacaatgct aatacaactg atacaagcat aacgataatg ataattctta aaactttatt
    14641 ttgcattttg tgtgcctcca aatgaataac ctttgtatta ataattacga cataagatat
    14701 aaaactttta acttgagacg atataactag caatcatcat taaggaatat aataaaaaac
    14761 atgagtaagt ttgcttatgt cattttcctc attatacgat atctttgaaa aatcaatcaa
    14821 tatatttttg acaaagctta gtttgagtta caataaatat atataataaa ttgaaaataa
    14881 tatgacacaa tttacaagcc tgataaatct gtgtaacatc aattactgta acattttatt
    14941 tcatcaattt ttaccgtttg gcgtacatga aaattttgat aaaaggggtt ccattatgat
    15001 taatgagcaa agattactta atacgttttt tgaacttgta caaatcgatt ctgagacagg
    15061 gaatgaatca acaatccaac ctattttaaa agaaaaattc atagcattag ggttagatgt
    15121 taaagaagat gaagcggcta aacatcctaa attaggtgcc aataatctag tgtgtacaat
    15181 gaatagtact atcgaagaag gcgaagtacc taaattatat ttgacgagcc atatggatac
    15241 tgttgtaccc gcaattaatg taaagccaat tgtaaaagat gacggctaca tatactctga
    15301 tggtacgact atactaggtg cagatgataa agcaggatta gcagcgatgc ttgaagtttt
    15361 gcaagtgata aaggaacaac aaatcccaca tggacaaatt caatttgtga ttactgttgg
    15421 ggaagaatca ggtttaattg gtgctaaaga attaaattca gagttgttgg acgcggactt
    15481 tggttatgct attgatgcaa gtgctgatgt cggcactacg gttgtaggtg caccgacgca
    15541 aatgttaatt tcagctaaaa tttttggcaa aacggctcat gcaagtacgc caaaagaggg
    15601 tgttagtgct attaatattg cggcgaaagc aattagtcga atgaaattag gacaagttga
    15661 tgaaataact actgccaata ttggtaagtt tcatggcggt tcagctacga atattgttgc
    15721 agatgaagtc attttagaag cagaggcacg ttctcacgat ccagaaagaa taaaaacaca
    15781 agtcaaacat atgactgatg tgtttgaaac tactgcatca gaattaggtg ggaaagcaga
    15841 agttacagtg gagcaaagtt atccaggatt taaaattaat gataatgaag cggtagttaa
    15901 aattgcacaa gaaagtgctc gtaatttagg attatctgca aatacaatta tttctggtgg
    15961 cggttcagac ggcagtatta ttaatacatt tggtattcct tcagtcattt taggagtggg
    16021 ttatgaaaaa atccatacaa caaatgagag aatgccgatt aaatcattga acttgcttgc
    16081 aagtcaagtg ttagaaataa tcaaaatagt tgcgcgacaa tcgaaatgat ttagtgtagt
    16141 gtatgtctat tttgtgatac aatactaatg aaaaatcaac aagtgaggta agtaatatga
    16201 cacaacaaat tggagttatt ggtttagccg ttatgggtaa aaacctagct tggaatattg
    16261 aatcacgcgg atatagtgta tctgtattta accgctcaag tgaaaaaact gatttaatgg
    16321 ttgaagaatc aaaagggaaa aatattcatc caacgtactc attggaagaa tttgttaatt
    16381 ctttagaaaa accacgtaaa attttattaa tggttcaagc aggtaaagct acggatgcaa
    16441 cgattgatag tttgttacct ttattagatg atggagatat tttaattgat ggtggtaata
    16501 caaactatca agatactatc agacgtaata aagcattagc acaaagtgca atcaacttta
    16561 ttggtatggg cgtttctggt ggtgaaattg gagcgttaac tggtccgtca ttaatgccgg
    16621 gtggacaaga agaagcttat aacaaagttg cagatattct tgatgcaatt gctgcaaagg
    16681 caaaagacgg tgcttcatgt gttacttata ttggaccaaa tggtgctgga cactatgtga
    16741 aaatggtaca taatggtatt gaatatgctg acatgcaatt aattgcagaa agttatgcta
    16801 tgatgaaaga attattaggc atgtctcatg aagacattgc tcaaacgttc aaagactgga
    16861 atgctggtga attagaaagt tatttaattg aaatcactgg tgatattttc atgaaattag
    16921 atgaaaataa agaagcatta gttgaaaaaa ttcttgatac tgcaggtcaa aaaggtacag
    16981 gaaaatggac ttcaattaac gcattagaat taggtattcc attaacaatc attactgaat
    17041 ctgtatttgc acgttttatc tcttcaatca aagaagagcg cgtaaatgct tctaaagaat
    17101 taaatggtcc aaaagcatca tttgatggtg acaagaaaga ctttttagaa aaaattcgta
    17161 aagcattata tatgagtaaa atttgttctt atgcacaagg tttcgcgcaa atgcgtaaag
    17221 caagtgaaga taatgaatgg aatttaaaac ttggtgattt agctatgatt tggagagaag
    17281 gttgcattat ccgtgcacaa ttcttacaaa agatcaaaga tgcttacgat aataatccag
    17341 gattacaaaa cttattgcta gatccttact ttaaaaacat cgttactgaa tatcaagatg
    17401 cgttacgtga tgttgtcgca actggtgttc aaaatggtgt accaacacct ggtttctcat
    17461 caagtattaa ttactatgat agttaccgtg cagctgattt acctgcaaac ttaattcaag
    17521 cacaacgtga ctacttcggt gctcatacgt acgaaagaaa agataaagaa ggcgtattcc
    17581 atacacaatg gattgaagaa taatattgat taaaggatag ttgaaagata ctatcctttt
    17641 ttatatcttt tgataaatat aaaagagagt gtgatagagt agctacaacc tataaaaaat
    17701 tacacaagca atatctatta ccttaaaagc atcccgccat atttttaagc attttgtgaa
    17761 gtaataaaat atttactgtg ttatgatagc atgtactata gagaaataga ggtattaact
    17821 tatggaaaag ttaaatcaag ttatgttata tgttgatgat gttgacaaag cgaaagcatt
    17881 ttggacagaa actttagaat ttgttgttgt aagtgaaaca ccgttagctg aggactatgt
    17941 tgcagttgaa gtatcaccta cgaaagatgc tgaaacgtca ttgacgatta tggcaaaaga
    18001 atttattgaa aagtatagcc cggaagtgaa cctaggaaca ccatctttaa tgtttaaaga
    18061 gaaaaacttt gatgcattat actcaaaatt aaatgattta ggtttaactg ggcatgatat
    18121 agtagaaatg aatggtcaac gtgtatttaa ttttcaagat ggccaaggta actattttgc
    18181 agttagtgac taaacaatcg attttaatat ttatacaaac ctatatattg attttcggat
    18241 tgaatttcag tatgaaggtt ttatttttat cgttttatgc aatcgtttgc acaaatcttt
    18301 tgactttttt agaaagtgtg actaaactgg tattatacag tggttggaag cgttttccga
    18361 tgcagaaatt aacaggaggt gattaaatgg ttacgattaa agatgttgca ctaaaagccg
    18421 gtgtttctcc ttcgacagtt tcaagagtta taaaaggaaa tcaacgtatt agcgaagcga
    18481 caatttcaaa agtgaagaaa gttatggaag aattgaatta ttttcctaat accgctgcta
    18541 gaactttaat tacaaaccaa acatataaaa ttggtttagt gttaaaaggg tctgaggagc
    18601 ctattcgact gaatccattc tacatcaatg tattgctagg aatttctgaa acgtgtaacc
    18661 agcatggcta tggtacacaa acgacagtct caaataatat gaatgattta atggatgaag
    18721 tttataaaat gattaaacaa cgaatggttg atgcgtttat actgctttat tcaaaagaaa
    18781 atgatccaat taaacaaatg ttaattgatg aaagcatgcc atttattgtg attggtaagc
    18841 ctacatcgga tatagatcat caatttacac acatagataa tgataatata ttagcttctg
    18901 aaaatttgac acgacatgtt attgaacaag gtgtagatga attaatattt attacagaaa
    18961 aaggaaattt tgaagtttca aaagatagaa ttcaaggatt tgaaacggtt gcatcacaat
    19021 ttaatctgga ttatcaaatt attgagacta gtaatgaaag aaaagttatt tttaattaca
    19081 tgcaaaatct acatacgcgc ttgaaggacc caaatattaa acaggcaatc atttcgttag
    19141 atgctatgtt acatttagcg attttaagtg tgctatatga acttaatatt gaaattccaa
    19201 aagatgtaat gacagcaaca ttcaatgatt cttatttaac cgaaattgcg tcaccacctc
    19261 aaaccagtat tgacatcaag cctcgaatgt taggtcaaca ggctggttca gcaattttaa
    19321 atatattgaa aaacaaagca caggatgtta ttgaactcgt cattatagat acagaattaa
    19381 aaataagaaa atcaacacag cgatagcaaa ggagttttaa aatgaataag caatggtgga
    19441 aagaagcagt agcatatcaa gtatatccaa gaagttttaa tgatagtaat cacgatggta
    19501 ttggggattt acctggaatg attgataaat tggactactt aaaagattta ggtatcgatg
    19561 tcatttggct cagtccaatg tttaaatcac ctaatgatga caatggttat gatattagtg
    19621 actatcaaga aattatggat gaatttggaa cgatggaaga ctttgattgt ttattaaaag
    19681 gtgttcatga tagaggcatg aagcttattt tagatttagt tgtaaatcat acatctgatg
    19741 aacatccttg gtttatagaa tccaaatcta gtaaagacaa tcccaaacgt gattggtaca
    19801 tttggcaaga tccaaagcca gatggctctg aacctaacaa ctgggaaagt atatttaatg
    19861 gatctacatg ggaatatgat gctaatactg agcaatatta tttccattta ttcagtaaaa
    19921 aacaacctga tttgaattgg ggtaatccgg aagttagaga tgctgtattt gaaatgatga
    19981 actggtggtt tgataaaggc attgatggat ttagagtaga tgcaattacg catattaaga
    20041 agacgtttga agcgggtgac ttacctgtac ctgagggtaa aacatatgcc ccagcatttg
    20101 atgtagatat gaatcagcca ggtatacaaa cttggttaca agagatgaaa gatcgctcat
    20161 taagtaagta tgacattatg actgttggtg aagcgaatgg tgtaagccct gatgatgctg
    20221 atgactgggt cggggaagaa aatggtaaat ttaatatgat attccaattt gaacatttgg
    20281 gactgtggaa tagtggtgat tctcactttg atgtaaattc gtataaatct gtattaaata
    20341 gatggcaaaa acaacttgaa aataaaggtt ggaatgcgtt gtttattgaa aatcacgacc
    20401 aaccacgacg tgtatcgacg tggggtgacg atgacaagta ttggtatgaa tcagcaacaa
    20461 gtcatgcagc tgtttatttc ttgcaacaag gtacgccatt catttatcaa ggtcaagaaa
    20521 ttggtatgac gaattatcca tttgaaagta ttgaaacgtt taacgatgtt gctgttaaaa
    20581 atgactatca aatagtgaaa gctcaaggtg gagatgtaga cgctttactt gcgaaatata
    20641 aagatgagaa ccgagataat tctcgcacac caatgcaatg ggatgatacg ttaaatggag
    20701 gatttacaaa tggtgaaccg tggttcccag tgaatccgaa ttataaaact atcaatgttg
    20761 cacaacaatt agaagatgag cattcagtat tacaatttta taaagattta attcaattaa
    20821 gaaagtctaa tgatgtatac gtatatggtc aatttgattt agtagatgct gaaaattcac
    20881 aagtttttgc gtacacgaga acattaaatg aaaagcaagt tcttattgta ggtaatctta
    20941 ctaaccacga agcagaatta actgtaccat ttgatttaag ccatggagaa gtaaagctat
    21001 ttaattatga tgccaaagtt aatttaaaac agttacgtcc atatgaagca tgtgttatcg
    21061 aactaaatta acatattaat atgcccccta atagaatcta aggccatttg tagacctata
    21121 gatatattag ggggcttctt tgtttttaat ttttaatctt cgtcataagg actctgttta
    21181 acaggaatcc aaagctgaat tttagtgaat gggtcattaa atgaaatatc gagagggtac
    21241 acttcaacat ataaatcatt tcgttcatat ggcaatgtta actgcaaact tgattcaata
    21301 tagtaccaag cttcattagt tgcataatca atttcacctt gtaaattgaa tttcgcacaa
    21361 tgtttgccag gtaaaaatcg gctttctaag tgtgcaggat aacgttcact tggtacacct
    21421 acaaatattt ctaaaccatt ttcaagagga caactaataa caaatagttc aaatggacta
    21481 acgtcattat atcgtcgtaa ctctttaatt ttaccatcaa tgagcaagtc ttctaaaaaa
    21541 tccggaacat taaaaggatg tgacaaatac ttagtgtcta taaatcgtgc atatccaacc
    21601 aatgaaatat catctgtctc ttctaatctg tatggatagg gtgttctctc agtagttgat
    21661 aatttgatat ataatcgctc ttgaatttgt aattcatctt ttttagtaga agcttgaata
    21721 ggtgatacgc cgtgaaaatc actaaaatca tttgcaaaat catttgaatt tgcatagtga
    21781 tattttttag cgatatctac aagtcgtgta gcaccattaa tcacatcatt tgcagcgagt
    21841 gtcattttac gcgcgcgtgc ataagcttct ggagataagc cgacaatcat tttaaatgat
    21901 tgatcaagat ggtatggcga aagaccaacg taatcactta attcttgcaa attgaaaggc
    21961 tctaataaac gatcttcaat ataaacaatt gcctgttgta tttgcttgat aacgtccaaa
    22021 actttcactc caattaattc aatcatttat ttttattcta cattatttct ataaattata
    22081 cacccatttg ttcaatgatt attaaaatag ttttgggcat tgtaaaatat aatttcataa
    22141 tatagtctag aaaaaaagcg aatgatagaa caattgattt acttgattcg taatcaatcc
    22201 ttgtcattcg ctcatttatt tttgtttaac atgtgcgttt taattcaatt attgaatatc
    22261 gtcccaccaa tggttaccat cacgagcaag tagtaaatca ctttctaatg gaccattagt
    22321 acctgattca tagttaggga attctggatc aaccatattc cattcatctt gaattgcatc
    22381 aacaaatttc catgttgatt ttaattcttc ccagtgcgtg aagttagtgg catcaccttt
    22441 aagacaatcg aataatagat tttcatatgc atctacagta ttcattttat cttgagcgct
    22501 cattgaataa gacaattgaa caggttcagt ttcgatacct tgtgtatttt tcttagcatt
    22561 tagatgtaaa gatacacctt cattaggttg gatattgatt actaataggt ttgaatctaa
    22621 tagtttatca gtttcatagt ataagttcat tggtacttct ttaaattcaa cgacaacttg
    22681 aattgtttta gatttcatac gtttaccagt acgaatatag aatggtacac cagcccatct
    22741 aaagttatca attgttaatt tacctgaaac aaaggtaggt gtgttagagt catctgcaac
    22801 gcgatcttca tcacggtatg ctttaacttg tttaccatcg atatagcctt cgccatattg
    22861 accacgaaca aagttctttt taacatcttc agattggaaa tgacgcagtg atttaagtac
    22921 ttttactttc tcagcacgga tatcttcact atttaaacta ataggtgctt ccatagctaa
    22981 taatgcaacc atttgtaaca tgtggttttg caccatatct tttagcgcgc cacttgattc
    23041 ataataacca ccacgatctt caacacctag tatttcagat gatgtaactt ggatgtttga
    23101 aatatatttg ttattccata atggttcaaa catcgcattc gcaaaacgta atacctcgat
    23161 attttgaacc atgtcttttc ctaaatagtg gtcaatacga taaatttctt cttctttaaa
    23221 tgatttacga atttgattgt ttaatgcttc ggctgatttt aaatcactac cgaatggttt
    23281 ttcgataaca aggcgtttaa atccttttgt atcagtaaga ccagaagatt ttagataatc
    23341 agaaataacg ccaaagaatt gtggtgccat tgctaaatag aatagtcgat taccttttaa
    23401 ttcaaattgg ctatctaatt cattactaaa atctagtaat tcttgatagc tttcttcatt
    23461 actaacatca tgtctatgat agaagacatg ttccataaac gcgtcaattt tgtttgtatc
    23521 ttttacgtgc ttttgaattg atgattttac ttgattacgg aaatcatcat tagtaatgtc
    23581 acgacgtcca ataccgatga tggcaatatg ttcatctaaa ttgtcttgtt ggtagagatg
    23641 gaatattgat ggaaacaact tacgatggct taagtcacca gttgcaccaa agattgtgat
    23701 taaacaaggg atgtgtttgt ttttagtact caagattaaa acctcaattc ttaattagat
    23761 atatgattta ttattataag ataatccatt cagcaggtca ttcaatatgc tcgacagtat
    23821 gcagtgtctt aaatgaaata tcgattcatg tatcat
//
LOCUS       DAMZLE010000027        23617 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMZLE010000027 DAMZLE010000000
VERSION     DAMZLE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.027"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..227
                     /locus_tag="POB92_000962"
     CDS             <1..227
                     /locus_tag="POB92_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDI6578472.1"
                     /translation="ETYEFYCRLRDELKNSDLMIEHTNKAGASNIVKNPLSIELTKTV
                     QTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            217..1908
                     /locus_tag="POB92_000963"
     CDS             217..1908
                     /locus_tag="POB92_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI6578473.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            1913..3151
                     /locus_tag="POB92_000964"
     CDS             1913..3151
                     /locus_tag="POB92_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6578474.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3135..3908
                     /locus_tag="POB92_000965"
     CDS             3135..3908
                     /locus_tag="POB92_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDI6578475.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            3920..5083
                     /locus_tag="POB92_000966"
     CDS             3920..5083
                     /locus_tag="POB92_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6578476.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            5152..5430
                     /locus_tag="POB92_000967"
     CDS             5152..5430
                     /locus_tag="POB92_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDI6578477.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5442..5774
                     /locus_tag="POB92_000968"
     CDS             5442..5774
                     /locus_tag="POB92_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578478.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            5771..6172
                     /locus_tag="POB92_000969"
     CDS             5771..6172
                     /locus_tag="POB92_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578479.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            6173..6568
                     /locus_tag="POB92_000970"
     CDS             6173..6568
                     /locus_tag="POB92_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDI6578480.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            6603..7244
                     /locus_tag="POB92_000971"
     CDS             6603..7244
                     /locus_tag="POB92_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6578481.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            7336..7791
                     /locus_tag="POB92_000972"
     CDS             7336..7791
                     /locus_tag="POB92_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDI6578482.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            7849..8199
                     /locus_tag="POB92_000973"
     CDS             7849..8199
                     /locus_tag="POB92_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578483.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            8241..8399
                     /locus_tag="POB92_000974"
     CDS             8241..8399
                     /locus_tag="POB92_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578484.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            8413..14613
                     /locus_tag="POB92_000975"
     CDS             8413..14613
                     /locus_tag="POB92_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6578485.1"
                     /translation="MNEKVEGMTLELKLDHLGVQEGMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            14613..15437
                     /locus_tag="POB92_000976"
     CDS             14613..15437
                     /locus_tag="POB92_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6578486.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            15446..17029
                     /locus_tag="POB92_000977"
     CDS             15446..17029
                     /locus_tag="POB92_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6578487.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFNCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            17029..17319
                     /locus_tag="POB92_000978"
     CDS             17029..17319
                     /locus_tag="POB92_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578488.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            17335..19245
                     /locus_tag="POB92_000979"
     CDS             17335..19245
                     /locus_tag="POB92_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578489.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            19245..20711
                     /locus_tag="POB92_000980"
     CDS             19245..20711
                     /locus_tag="POB92_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDI6578490.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            20711..21100
                     /locus_tag="POB92_000981"
     CDS             20711..21100
                     /locus_tag="POB92_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDI6578491.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            21093..21257
                     /locus_tag="POB92_000982"
     CDS             21093..21257
                     /locus_tag="POB92_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDI6578492.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            21303..21602
                     /locus_tag="POB92_000983"
     CDS             21303..21602
                     /locus_tag="POB92_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDI6578493.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            21738..22040
                     /locus_tag="POB92_000984"
     CDS             21738..22040
                     /locus_tag="POB92_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6578494.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            22051..23505
                     /locus_tag="POB92_000985"
     CDS             22051..23505
                     /locus_tag="POB92_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI6578495.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT         9031 a         3220 c         4779 g         6587 t
ORIGIN      
        1 tagaaacgta tgaattttat tgtcggttaa gagatgaact taaaaatagt gatttgatga
       61 tagagcatac aaacaaggct ggtgcgagca atattgttaa gaatccatta agcatagaac
      121 tgacaaaaac agttcaaaca ctaaataact tactcaagtc tatgggttta actgcagcac
      181 aaagaaaaaa gatagttcaa gaagaaggtg gattcggtga ctattaaagt tttaaatgaa
      241 ccttcaccaa aactattaac aacatggtat gcagagcaag tcactcaagg gaaaataaaa
      301 acaagcaaat atgttaaaaa agaatgtgag agacacctta gatatctaga aaatggaggt
      361 aaatgggtat ttgatgaaga attagcgcac cgtcctattc gattcataga aaagttttgt
      421 aaaccttcca aaggatctaa acgtcaactt gtattacaac catggcaaca ttttattatt
      481 ggcagtttgt ttggttgggt tcataaagaa acaaaactgc gcaggtttaa agaagctttg
      541 atatttatgg ggcgaaaaaa tggtaaaaca actactatat ctggtgttgc taactatgct
      601 gtttctcaag atggagaaaa cggcgctgaa atccatcttt tagcaaacgt aatgaaacaa
      661 gctagaatat tattcgatga atctaaggcg atgataaaag ctagcccaaa gcttgataaa
      721 aatttcagaa cattaagaga tgaaatccat tatgacgcaa cgatatcaaa aattatgccc
      781 caagcatcag atagcgataa gttagatgga ttgaatacac acatggggat ttttgatgaa
      841 attcatgaat ttaaagacta taaattgatt tcagttataa aaaactcaag agctgcaagg
      901 ttacaacctc ttctcatcta cattacgaca gcagggtatc aattagatgg tccacttgtt
      961 gatatggtag aagcgggaag agacacctta gatcaaatca tagaagacga aagaactttt
     1021 tattatttag catctttgga tgatgacgat gatattaatg attcgtcgaa ctggataaaa
     1081 gcaaatccca acttaggtgt ctctataaat ttagatgaga tgaaagaaga gtgggaaaaa
     1141 gctaagagaa caccagctga acgtggagat tttataacca aaaggtttaa tatctttgct
     1201 aataatgacg agatgagttt tattgattac ccaacactcc aaaaaaataa tgaaattgtt
     1261 tctttagaag agctggaagg cagaccatgc acgattggtt atgatttatc agaaacagag
     1321 gactttacag ccgcgtgtgc tacttttgcg ttagataatg gtaaagttgc agttttatcg
     1381 cattcatgga ttcctaagca caaagttgaa tattctaacg aaaaaatacc ctatagagaa
     1441 tgggaagaag atggcttatt aacagtgcaa gataagcctt atattgacta ccaagatgtt
     1501 ttaaattgga taattaagat gaatgagcat tatgtagtag aaaaaattac ttatgataga
     1561 gcgaacgcat tcaaactaaa tcaagagtta aaaaattacg ggtttgaaac ggaagaaaca
     1621 agacaaggag ctttgacctt gagccctgca ttgaaggatt taaaagaaat gtttttagat
     1681 gggaaaataa tatttaataa taatccttta atgaaatggt atatcaataa tgttcagttg
     1741 aaactagaca gaaacggaaa ctggttgccg tctaagcaaa gcagatatcg taaaatagat
     1801 ggctttgcag catttttaaa cacatataca gatattatga ataaagttgt ttctgacaag
     1861 ggtgaaggaa acatagaatt tattagtatt aaagatataa tgcgttaagg aggtgaatgt
     1921 tatcgcaaaa gagaatattg tcacacgcat aaagaaaaaa ttgatagaca attggattga
     1981 tcagtcagct tctaagcttt atgactttag cccatggaaa aataaatctt tttggggtgt
     2041 aatcaataat acgcttgaaa ctaatgaaac gatattttca gctattacaa agttatctaa
     2101 ttcgatggct agtttgccct tgaaaatgta tgaagattat aaagtagtta atacagaagt
     2161 atctgattta cttacagtgt caccgaataa ttctctgagc agttttgatt ttattaatca
     2221 aattgaaaca atcagaaatg aaaaaggtaa tgcatatgtg ctaattgaac gagacatcta
     2281 tcatcaacca tcaaagcttt tcttattaaa tccagatgtt gttgaaatgt taattgaaaa
     2341 ccaatcacgt gaactttatt attccattca tgctgcaact ggaaataaat tgattgttca
     2401 taatatggac atgttgcatt ttaaacacat cgtggcatct aatatggtgc aaggcattag
     2461 tccgattgat gtgttgaaga atacaactga ttttgataat gcagtaagaa cctttaatct
     2521 tacagaaatg caaaaacctg attctttcat gcttaaatat ggttccaatg taggtaaaga
     2581 aaaaaggcag caagtgttag aagatttcaa acagtactat gaagaaaacg gtggaatatt
     2641 attccaagag cctggtgttg aaatcgaacc gttacctaaa aaatatgtct ctgaagatat
     2701 agtggcaagc gagaatttaa caagagaaag agtagctaac gtttttcaat tgccctcagt
     2761 attcttaaat gcaagatcaa atacaaattt cgcgaaaaat gaagagttaa acagatttta
     2821 cttgcagcat accttattgc caatcgtcaa acagtatgaa gaagaattta atcggaaact
     2881 acttactaaa acagacagag aaaaaaatag gtattttaaa tttaacgtta aatcttattt
     2941 aagggctgat agtgcaacac aagcagaagt gtactttaaa gcagttcgta gtggttacta
     3001 cactataaat gacattagag agtgggaaga tttaccacca gttgaaggtg gagataagcc
     3061 gctaataagc ggtgatttat acccaattga cacgccactt gaattaagaa aatctttgaa
     3121 aggtggtgat aaaaatgtca atgaaagcta agtattttca aatgaaaaga aaatcaaaaa
     3181 gtaaaggtga aatatttatt tatggtgata ttgtaagtga taaatggttt gaaagtgatg
     3241 taactgctac agatttcaaa aataaactag atgaactagg agacatcagt gaaatagatg
     3301 ttcatataaa ttcatctgga ggcagtgtat ttgaagggca tgcaatatac aatatgctaa
     3361 aaatgcatcc tgcaaaaatt aatatctatg tcgatgcctt agcggcatca attgctagtg
     3421 ttatcgctat gagtggtgac actattttta tgcacaaaaa tagtttttta atgattcata
     3481 attcatgggt tatgactgta ggtaatgcag aagaattaag aaagacagcg gatttacttg
     3541 aaaaaacaga tgctgttagt aattcagctt atttagataa agcaaaagat ttagatcaag
     3601 aacacttaaa acagatgtta gatgcagaaa cttggcttac tgcagaagaa gccttgtctt
     3661 tcggcttgat agatgaaatt ttaggagcta atgaaatagc tgctagtatc tctaaagagc
     3721 aatataagcg tttcgagaac gtcccagaag atttaaagaa agatgtagac aaaatcacta
     3781 aaattgatga tgtagataca tctgaattgg ttgaaacacc taaagaaagt atgtcactag
     3841 aagaaaaaga aaaaagagaa aaaattaaac gcgaatgcga aattttaaaa atgacaatga
     3901 attattagga ggaaatgaaa tgccgacatt atatgaatta aaacaatcct taggtatgat
     3961 tggacaacaa ttaaaaaata aaaatgatga attgagtcag aaagcaacag atccaaatat
     4021 tgatatggaa gacatcaaac aactagaaac agaaaaagca ggtttacaac aaagatttaa
     4081 cattgttgaa agacaagtgc aagacattga agagaaagaa aaagcgaaag ttaaagataa
     4141 aggagaagct tatcaatctt taagtgataa tgagaagatg gttaaagcta aggcagagtt
     4201 ttatcgtcac gcgattttac caaatgaatt tgaaaaacct tcaatggagg cacaacgttt
     4261 attacacgct ttaccaacag gaaatgattc aggtggagat aagctcttac caaaaacact
     4321 ttctaaagaa attgtttcag aaccatttgc taaaaaccaa ttacgtgaaa aagctcgtct
     4381 aactaacatt aaaggtttag agattccaag agtttcatac actttagacg atgatgattt
     4441 cattacagac gtagaaacag caaaagaatt aaaagcaaaa ggtgatacag tcaagttcac
     4501 tactaataaa ttcaaagtat ttgctgcaat ttcagatact gtaattcatg gatcagatgt
     4561 agatttagta aactgggttg aaaacgcact acaatcagga ttagcagcta aagagcgtaa
     4621 agatgcctta gcagtaagtc ctaaatctgg attagaacac atgtcatttt ataatggatc
     4681 tgttaaagaa gttgagggag cagacatgta tgatgctatt attaacgctt tagcagattt
     4741 acatgaagat tatcgtgata acgcaacaat ttatatgcga tatgcagatt atgtcaaaat
     4801 tattagtgtt ctttcaaatg gaacaacaaa tttctttgac acaccagcag aaaaagtatt
     4861 tggcaaacca gtagtattta cagatgcagc agttaaacct attgtgggag atttcaatta
     4921 ttttggaatt aactatgatg gaacaactta tgacactgat aaagatgtta aaaaaggcga
     4981 atatttgttt gtattaacag catggtatga tcagcaacgt acattagaca gtgcattcag
     5041 aattgcaaaa gcaaaagaaa atacaggtcc attacccagc taagccccaa aaggttaatg
     5101 taacagctaa ggctaaatca gctgtaatat cagccgaata ggggtgatga aatgagttta
     5161 gaagaaatta aattgtggtt gagaattgac tataatttcg aaaatgattt aattgaaggt
     5221 ctcattcaat cggctaagtc tgaattacta ttaagtgggg ttccagatta tgacaaagat
     5281 gacttggaat acccgctttt ttgtacagcg attaaatata tcattgcaag agattatgaa
     5341 agtcgtggat actcaaatga ccaatctaga agcaaggtgt ttaatgaaaa aggattgcaa
     5401 aaaatgattt tgaaattaaa aaagtggtag gtgattttta aatggaattt aatgaattta
     5461 aagatcgcgc gtattttttt caatatataa acaaaggacc atatccagat gaagaggaaa
     5521 aaatgaaatt gtatagttgc ttttgtaaaa tttataatcc ttctatgaaa gatagagaaa
     5581 ttttaaaagc gactgaatca aaatcaggat taaccataat tgtcaggtct tctaaaactg
     5641 aatatctacc acaaacaaat cacttagtta aaattgacag tgcattatat tccgataaat
     5701 tattcaacat tgtagaaata agaattgata caccagatat tggctataat acagtggttt
     5761 tatcagaaaa atgagtgtag aaattaaagg gatacctgaa gtgttgaata aattagaatc
     5821 ggtatacggt aaacaagcaa tgcaggctaa gagtgataaa gctttaaatg aagcatctga
     5881 attttttata aaggctttaa agaaagagtt cgagagcttt aaagatacgg gtgccagtat
     5941 agaagaaatg actaaatcta agccttatac aaaagttggc agtcaagaaa gggctgtttt
     6001 aattgaatgg gtaggcccta tgaatcgcaa aaacattatt cacttgaatg aacatggtta
     6061 tacaagagat ggaaaaaaat atacaccaag aggttttgga gttattgcaa aaacattagc
     6121 tgctagcgaa cgtaagtata gagaaattat aaaaaaggag ttggccagat aaatgaatat
     6181 attaaacacc ataaaaggaa ttttattatc tgatgcagag ctcaaaacac atataaattc
     6241 tagaatatac tattacaaag tcactgaaaa cgctgaaact tccaaaccct ttgttgttat
     6301 tacacctgtt tatgatttgc cttcagactt tatgtctgat aaatatctca gtgaagaata
     6361 cttaattcaa atagatgtag aatcttcaaa taatcagaaa acaattgata taacaaaacg
     6421 aataagatac ctgttatatc aacaaaattt aattcaagca tctagtcagt tagatgctta
     6481 ttttgaagaa actaaacgtt atgtgatgtc gagacgatat caaggcatac ccaaaaatat
     6541 atattataaa aatcagcgca tcgaataggt gtgcttttta atttttaagg aggaaataag
     6601 caatggcaga aggacaaggt tcttataaag taggttttaa aagattatac gttggagttt
     6661 ttaacccaga agcaacaaaa gtagttaaac gcatgacatg ggaagatgaa aaaggtggta
     6721 cagttgacct aaatatcaca ggtttagcac cagatttagt agatatgttt gcatctaaca
     6781 aacgtgtatg gatgaaaaaa caaggtacta atgaagttaa gtctgacatg agtattttca
     6841 atattccaag tgatgattta aacacagtta ttggacgtac taaagataaa aatggtacat
     6901 cttgggtagg agagaataca agagcaccgt atgtaacagt aattggcgaa tcggaagatg
     6961 gtttaacagg tcagccggta tatgtagcct tacttaaagg tacttttagt ttagattcaa
     7021 ttgaatttaa aacacgaggt gaaaaagcag aagccccaga acctacaaaa ttaacaggtg
     7081 actggatgaa tagaaaagtt gatgttgatg gaacgtcaca aggtattgta tacggttatc
     7141 atgaaggtaa agaaggagaa gcagaattct tcaaaaaagt attcgttgga tacacggaca
     7201 gtgaagatca ttcagaggat tctgcaggtt cgttacccag ctaatcccca aaatgttgaa
     7261 gtagcagtta attcaaaatc tgcaacagtt tcagcagaat aggggctttc aaaataaatc
     7321 aaaggagaat aatttatgac taaaacttta aaggtttata aaggagacga cgtcgtagct
     7381 tctgaacaag gtgaaggcaa agtgtcagta actttatcta atttagaagc ggatacaact
     7441 tatccaaaag gtacttacca agtggcatgg gaagaaaatg gtaaagaatc tagtaaagtt
     7501 gatgtacctc aattcaaaac caatccaatt ctagtctcag gcgtatcatt tacaccagaa
     7561 actaaatcaa ttatggtaaa taccgatgac aatgttgagc caaacattgc accaagcaca
     7621 gcaacgaata aaatattgaa atatacaagt gaacatccag aatttgttac tgtagatgaa
     7681 aatacaggag caattcacgg tgtagctgaa ggtacttcag taatcactgc tatgtctact
     7741 gatggaagcg ataagtcagg acaaatttca gtgacagtaa caaacggata gggatttaag
     7801 gcgcagtata tctgcgtctt ttttatttga ataaaaggag ctaatacaat gattaaattt
     7861 gaaattaaag atcgtaaaac aggaaaaaca gagagctata caaaagaaga tgtaacaatg
     7921 ggcgaagcag aaaaatgcta tgagtattta gaattagtaa atcaagagaa taaaaaagaa
     7981 gcacctaacg caacaaaaat gagacaaaaa gagcgacagt tattagtaga tttatttaaa
     8041 gatgaaggat tgactgaaga agatgttctg aacaagatga gtactaaaac ttatacaaaa
     8101 gccttacaag atatatttcg agaaatcaat ggtgaagatg aagaagattc agaaactgaa
     8161 ccagaagaga tgggaaagac agaagaacaa tctcaataaa agacatttta tcgaacatta
     8221 agaaaataca acgtttctgt atggagcagt atgggtggac attaactgaa gtcagaaaac
     8281 agccgtatgt aaaactttta gaaatactta atgaagagaa taaagaagag actgaagaaa
     8341 aacaaagtga acaaaaagtc attacaggta cggatttaag aaaacttttt ggaagctaga
     8401 aaggaggtta atatgaatga aaaagtagaa ggcatgacct tggagctgaa attagaccat
     8461 ttaggtgtcc aagaaggcat gaaaggttta aagcgacaat taggtgttgt taatagtgaa
     8521 atgaaagcta atctgtcagc atttgataag tctgaaaaat caatggaaaa atatcaggcg
     8581 agaattaagg ggttaaatga taggcttaaa gttcaaaaaa agatgtattc tcaagtagaa
     8641 gatgagctta aacaagttaa cgctaattac caaaaagcta aatccagtgt aaaagatgtt
     8701 gagaaagcat atttaaagtt agtagaagcc aataaaaaag aaaaattagc tcttgataaa
     8761 tctaaagaag ccttaaaatc atcgaataca gaacttaaaa aagctgaaaa tcaatataaa
     8821 cgtacaaatc aacgtaaaca agatgcgtat caaaaactta aacagttgag agatgcagaa
     8881 caaaagctta agaatagtaa ccaagctact actgcacaac taaaaagagc aagtgacgca
     8941 gtacagaagc agtccgctaa gcataaagca cttgttgaac aatataaaca agaaggcaat
     9001 caagttcaaa aactaaaagt gcaaaatgac aatctttcaa aatcaaatga taaaattgaa
     9061 agttcttacg ctaaaactaa tactaaatta aagcaaacag aaaaagaatt taatgattta
     9121 aacaatacta ttaagaatca tagcgctaat gtcgcaaaag ctgaaacagc tgttaataaa
     9181 gaaaaagctg ctttaaataa tttggagcgt tcaatagata aagcttcatc cgaaatgaag
     9241 acttttaaca aagaacaaat gatagctcaa agtcatttcg gtaaacttgc aagtcaagcg
     9301 gatgtcatgt caaagaaatt tagttctatt ggagacaaaa tgacttccct gggacgtaca
     9361 atgacgatgg gcgtatctac accaattact ttagggttag gtgcagcatt aaaaacaagt
     9421 gcagactttg aaggccaaat gtctcgagtt ggagcgattg cgcaagcaag cagtaaagac
     9481 ttgaaaagca tgtctaatca agcagttgac ttaggagcta aaaccagtaa aagtgctaac
     9541 gaagttgcta aaggtatgga agaattggca gctttaggct ttaatgccaa acaaacaatg
     9601 gaggctatgc caggtgttat cagtgcagca gaagcaagtg gtgcagaaat ggctacaact
     9661 gcaactgtaa tggcttcagc gattaactct ttcggtttaa aagcatctga tgcaaatcat
     9721 gttgctgatt tacttgcgag atcagcaaat gatagtgctg cagatattca gtacatggga
     9781 gatgcattga agtatgctgg tactcctgca aaagcattag gagtttcaat agaggacact
     9841 tccgcagcaa ttgaagtttt atctaactca ggtttagagg gttctcaagc aggtactgcc
     9901 ctaagagctt catttatcag gctagctaat ccaagtaaaa atacagctaa ggaaatgaaa
     9961 aaattaggta ttcatttgtc tgatgctaaa ggtcaatttg ttggcatggg tgaattgatt
    10021 agacagttcc aagataatat gaaaggcatg acgagagaac aaaaactagc tacagtggct
    10081 acaatagttg gtactgaagc agcaagtgga tttttagcct tgattgaagc gggaccagat
    10141 aaaattaata gctatagtaa atccttaaag aattccaatg gcgaaagtaa aaaagcagca
    10201 gatttgatga aagataatct caaaggcgct ctggaacaat taggtggcgc ttttgaatca
    10261 ttagcaatcg aagtcggtaa agatttaacg cctatgatta gagcaggagc ggaaggttta
    10321 acaaaattag ttgatggatt tacacatctc cctggttggg ttagaaaagc ttcagtagga
    10381 ttagcacttt ttggtgcagc aattggacct gcagttcttg ctggagggtt attaatacgt
    10441 acagttggaa gtgctgctaa aggatatgcg tcattaaata gacgtattgc tgaaaataca
    10501 atcctttcaa atactaattc aaaagcaatg aaatctttag gtcttcaaac attatttctt
    10561 ggttctacaa caggaaaaac gtcaaaaggc tttaaagggt tagccggagc tatgatgttt
    10621 aatttaaaac ctataaatgt tttgaaaaat tctgcaaagc tagcaatttt accgttcaaa
    10681 cttttgaaaa acggtttagg attagctgca aaatctttat ttgcagtaag tggaggcgca
    10741 agatttgcgg gtgtagcctt aaggttttta acaggaccta taggtgctac aataactgct
    10801 attacaattg cgtataaagt ttttaaaacc gcatatgatc gtgtggaatg gttcagaaac
    10861 ggtattaacg gtttaggaga aactataaag ttttttggtg gtaaaattat tggcggcgct
    10921 gttagaaagc taggagagtt taaaaactat cttggaagta tcggcaaaag cttcaaagaa
    10981 aagttttcaa aagatatgaa agatggttat aaatcattaa gcgacgatga ccttctcaaa
    11041 gtaggagtca acaagtttaa aggatttatg caaaccatgg gcacagcttc taaaaaagcg
    11101 tctgatactg taaaagtgtt agggaaaggt gtttcaaaag aaacagaaaa agctttagaa
    11161 aaatatgtgc attattctga agaaaatagc agaatcatgg aaaaagtacg tttaaactcg
    11221 ggtcagatat cagaagacaa agcaaaaaaa cttttgaaaa ttgaaacgga tttatctaat
    11281 aaccttatag ctgaaataga aaaaagaaat aaaaaggaac tcgaaaaaac tcaagaactt
    11341 attgataagt atagtgcatt cgatgaacaa gaaaagcaaa acattttaac tcgaactaaa
    11401 gaaaaaaatg acttgcgaat taaaaaagag caagaactca atcagaaaat caaagaattg
    11461 aaagaaaaag ctttgagtga tggtcagatt tcagaaaatg aaagaaaaga aattgaaaag
    11521 cttgaaaatc aaagacgtga tatcactgtt aaagaattga gtaagactga aaaagagcaa
    11581 gagcgtattt tagtaagaat gcaaagaaac agaaatgctt attcaataga cgaagcgagc
    11641 aaagcaatta aagaagcaga aaaagcaaga aaagcaagaa aaaaagaagt ggacaagcaa
    11701 tatgaagatg atgtcattgc tataaaaaat aacgtcaacc tttctaagtc tgaaaaagat
    11761 aaattgttag ctattgctga tcaaagacat aaagatgaag taagaaaagc aaaatctaaa
    11821 aaagatgctg tagtagatgt tgttaaaaag caaaataaag atattgataa agaaatggat
    11881 ttatccagtg gacgtgtata taaaaatact gaaaagtggt ggaatggcct taaaagttgg
    11941 tggtctaact ttagagaaga ccaaaagaaa aaaagcgata aatacgctaa agaacaagaa
    12001 gaaacagctc gtagaaacag agaaaatata aagaaatggt ttggaaatgc ttgggacggc
    12061 gtaaaaagta aaactggcga agcctttagt aaaatgggca gaaatgctaa tcattttggc
    12121 ggcgaaatga aaaaaatgtg gagcggaatc aaagggattc caagcaaatt aagttcaggt
    12181 tggagctcag ccaaaagttc tgtaggatac cacactaagg ctatagctaa tagtactggt
    12241 aaatggtttg gaaaagcttg gcaatctgtt aaatcgacaa caggaagtat ttacaatcaa
    12301 actaagcaaa agtattcaga tgcttcagat aaagcttggg cgcattcaaa atctatttgg
    12361 agaggcacat caaaatggtt tagcaatgca tataaaagtg caaagggctg gctaacggat
    12421 atggctaata aatctcgcgc gaaatgggat aatatttcta gtacagcttg gtcgaatgca
    12481 aaatccgttt ggaaaggaac atcgaaatgg tttagtaact catacaaatc tttaaaagat
    12541 tggactgggg atatgtattc aagagcccac gatcgttttg atgcaatttc aagttcggca
    12601 tggtctaacg ctaaatcagt atttaatggt tttagaaaat ggctatccaa aacatatgat
    12661 tggattagag atattggtaa agacatggga agagctgcgg ctgatttagg taaaaatgtt
    12721 gctaataaag ctattggcgg tttgaatagc atgattggcg gtattaataa aatatctaaa
    12781 gccattactg ataaaaatct catcaagcca atacctacat tgtctactgg tactttagca
    12841 ggaaagggtg tagctaccga taattcggga gcattaacgc aaccgacatt tgctgtatta
    12901 aatgatagag gttctggaaa cgccccaggt ggtggagttc aagaagtaat tcacagggct
    12961 gacggaacat tccatgcacc ccaaggacga gatgtggttg ttccactagg agttggggat
    13021 agcgtaataa atgctaatga cactctgaag ttacagcgta tgggtgtttt accaaagttt
    13081 catggaggta cgaaaaagaa agattggcta gaccaactta aaggtaatat aggtaaaaaa
    13141 gcaggagaat ttggagctac agctaaaaac acagcgcata atatcaaaaa aggtgcagaa
    13201 gaaatggttg aagcagcagg cgataaaatc aaagatggtg catcttggtt aggcgataaa
    13261 atcggcgatg tgtgggatta cgtacaacat ccagggaaac tagtaaataa agtaatgtca
    13321 ggtttaaata ttaattttgg aggcggagct aacgctacag taaaaatagc taaaggcgca
    13381 tactcattgc tcaaaaagaa attagtagac aaagtaaaat cgtggtttga agatttcggt
    13441 ggtggaggcg atggaagcta tctatttgaa tatccaatct ggcaaagatt tggacgctac
    13501 acaggtggac ttaactttaa tggcggtcgt cactatggta tagactttgg tatgccttct
    13561 ggaacaaacg tttatgccgt taaaggtggt atagcagata aggtatggac tgattacggt
    13621 ggcggtaatt ctatacaaat taagactggt gctaatgaat ggaactggta tatgcattta
    13681 tctaagcaat tagcaagaca aggccaacgt attaaagctg gtcaactgat agggaaatca
    13741 ggtgctacag gtaatttcgt tagaggagca cacttacatt tccaattgat gcaagggtca
    13801 catccaggga atgatacagc taaagatcca gaaaaatggt tgaagtcact taaaggtagt
    13861 ggcgttcgaa gtggttcagg tgttaataag gctgcatctg cttgggcagg cgatatacgt
    13921 cgtgcagcaa aacgaatggg tgttaatgtt acttcgggtg atgtaggaaa tattattagc
    13981 ttgattcaac acgaatcagg aggaaatgca ggtataactc aatctagtgc gcttagagac
    14041 atcaacgttt tacagggcaa tccagcaaaa ggattgcttc aatatatccc acaaacattt
    14101 agacattatg ctgttagagg tcacaacaat atatatagtg gttacgatca gttattagcg
    14161 ttctttaaca acagatattg gcgctcacag tttaacccaa gaggtggttg gtctccaagt
    14221 ggtccaagaa gatatgcgaa tggtggtttg attacaaagc atcaacttgc tgaagtgggt
    14281 gaaggagata aacaggagat ggttatccct ttaactagac gtaaacgagc aattcaatta
    14341 actgaacagg ttatgcgcat catcggtatg gatggcaagc caaataacat cactgtaaat
    14401 aatgatactt caacagttga aaaattgttg aaacaaattg ttatgttaag tgataaagga
    14461 aataaattaa cagatgcatt gattcaaact gtttcttctc aggataataa cttaggttct
    14521 aatgatgcaa ttagaggttt agaaaaaata ttgtcaaaac aaagtgggca tagagcaaat
    14581 gcaaataatt atatgggagg tttgactaat taatgcaatc ttttgtaaaa atcatagatg
    14641 gttacaagga agaagtaata acagatttta atcagcttat atttttagat gcaagggctg
    14701 aaagtccaaa caccaatgat aacagtgtaa ctattaacgg agtagatggt attttaccgg
    14761 gcgcaattag ttttgcgcct ttttcattag tattaaggtt tggctatgat ggtatagatg
    14821 ttatagattt aaatttattt gagcattggt ttagatctgt gtttaatcgc agacatcctt
    14881 attatgttat tacttctcaa atgcctggtg ttaaatatgc agtgaataca gctaatgtta
    14941 catctaattt aaaagatggt tcttcaactg aaattgaagt aagtttaaat gtttataaag
    15001 ggtattctga atcagttaat tggaccgata gcgagttctt attcgactct aattggatgt
    15061 ttgaaaatgg aattcctctt gatttcacac ctaaatatac tcatacatca aatcaattta
    15121 ctatttggaa cggttctact gatacgataa atccacgatt caagcacgat ttgaaaatat
    15181 taattaattt aaatgcgagt ggaggatttg aactggttaa ctatacaaca ggtgatattt
    15241 ttaagtacaa caaaagtata gataaaaaca ctgattttgt tttagatggt gtgtatgcat
    15301 atcgagatat aaatagagtg ggaattgata caaatagagg cattataaca ttagcgccag
    15361 gtaaaaatga atttaagatt aaaggagacg tcagtgatat taaaactaca tttaagtttc
    15421 cttttattta taggtaggtg atttaatgga ttatcatgat catttatcag taatggattt
    15481 taatgaattg atttgtgaaa atttactaga tgtagattat ggttctttta aagaatatta
    15541 tgaactgaat gaagctaggt acatcacctt tacagtttat agaactactc ataatagttt
    15601 tgtttttgat ttattgattt gtgaaaactt cataatttat catggtgaaa aatatacaat
    15661 taagcagaca gcgccaaagg ttgaaggtga taaagttttt attgaagtta cggcatatca
    15721 cataatgtat gaatttcaaa atcactcagt ggaatcaaat aagcttgatg acgacagtag
    15781 cgaaactggt aaaacgccag aatactcttt agatgagtac ttaagatatg gatttgcaaa
    15841 tcaaaaaact tcggtcaaaa tgacctataa aataattgga gattttaagc gaaaagtacc
    15901 gattgacgaa ttaggtaaca aaaacggctt agaatactgt aaagaagcgg tagacctgtt
    15961 tggctgtata atttacccaa atgatacaga gattggtttt tattctcctg aaacatttta
    16021 tcaaagaagc gagaaagtga ttcgatatca atataatact gatactgtat ctgcaactgt
    16081 cagtacattg gaattaagaa cagctataaa agtttttgga aaaaagtata cagctgagga
    16141 aaagaaaaat tataatccta ttagaacaac tgacattaaa tattcaaatg gttttataaa
    16201 agaaggtact tatcgtaccg aaacaattgg gtctaaagct actattaact ttaattgcaa
    16261 gtatggtaat gaaacagtta gatttacaat aaaaaagggc tctcaaggtg gaatatataa
    16321 gttgatttta gacggcaagc aaattaagca aatttcttgt tttgctaagt cggttcagtc
    16381 tgaaacaata gatttaataa aaaatattga taaaggcaag cacgttttag aaatgatatt
    16441 tttaggagaa gaccccaaaa atagaattga tatatcttca aataaaaaag ctaagccttg
    16501 tatgtatgtt ggaactgaaa aatcaacagt cttaaattta attgctgata attcaggtcg
    16561 caatcaatac aaagcaattg tcgactacgt cgcagatagt gcaaagcagt ttgggattcg
    16621 atatgctaat acgcaaacaa atgaagatat cgaaacacag gataagctgt tagaatttgc
    16681 aaaaaagcaa ataaatgata ctcctaagac tgaattagat gttaattata taggttatga
    16741 aaaaatagag ccaagagata gcgtattttt tgttcatgaa ttaatgggat ataacactga
    16801 attaaaggtt gttaaacttg ataggtcaca tccatttgta aacgcaatag atgaagtgtc
    16861 tttcagcaat gaaataaaag atatggtaca aattcaacaa gcacttaaca gacgagttat
    16921 tgcacaagat aatagatata actatcaagc aaatcgtata aatcatttat acactagtac
    16981 tttgaattct cctttcgaga caatggatat agggagtgta ttaatataat ggcaacagaa
    17041 gaagttaaaa tcaaagcgct acttgaaaac gataaacagt actttccagc tacacactgg
    17101 aaagctataa atgggatacc ttatgcaggc agtagtgata ttgatggatt gcctcaagac
    17161 ggtatcattt cggtagatga taaaaataaa ttagataaat taaaaatagg cgaagcagga
    17221 attattcaaa atagcattgt acagaaatcc ccaaacggta aattgtggaa aataacagtt
    17281 gacgatagtg ggaaacttgg tacagtgcta ttttattaga aaggaaggtg cattatggaa
    17341 aatttgtatt taataaagga tttgggagct ttagcaggtc gagattatag agctaaagaa
    17401 attcaaaacc tgcaaagaat agagcaattt gcgcttggct tgacaacaga gtttaagttg
    17461 catcagaaag ctaaaacaat gcaacacttc gctgagcaaa tttattataa tggtagatcg
    17521 caagcagcag taaacaaatc tttacaaagt caaattaacg cacttgttgt ggcaccacgt
    17581 aataacagtg ctaatgagat tgttcaagct cgagttaatg taaacggcga aacctttgac
    17641 acattaaaag aacatttaga cgattgggaa accaaaactc aaattaataa agaggaaact
    17701 ataagagaat taaataagac caaacaagaa attcttgata tcgagtatcg ttttgaacct
    17761 gataagcaag agtttttatt tgtgacagaa cttgcacctc ttacaaatgc agtaatgcaa
    17821 tccttctggt ttgataatag aacaggcata gtatacatga cacaagctag aaataatggc
    17881 tatatgctaa gtcgtttaag acctaatggt caatttatag acagctcatt gattgtaggt
    17941 gggggtcatg gtacacataa cggttataga tatattgatg atgagttatg gatttatagt
    18001 tttatcttaa atggtaataa tgagaataca ttagttcgtt tcaagtatac gcctaatgtg
    18061 gaaattagct atggcaagta tggtatgcaa gatgtattta caggacaccc agaaaaaccc
    18121 tacatcaccc ctgtcataaa tgaaaaagaa aataaaattc tatacagaat tgagagacct
    18181 agaagtcagt gggaacttga aaactcaatg aattatatag agataagaag tttagacgat
    18241 gttgataaaa atattgataa agttttgcat aaaatcagta tccctatgag actaacaaac
    18301 gaaacccaac caatgcaggg tgtgactttt gatgaaaaat acttgtattg gtatacagga
    18361 gacagtaatc caaataatag aaactattta acggctttcg atttagaaac aggagaagaa
    18421 gcgtatcagg ttaatgctga ctatggtgga acactagatt catttcctgg cgaatttgcg
    18481 gaagcagaag gtttgcaaat atactatgac aaagatagtg gtaaaaaagc tttgatgcta
    18541 ggtgttactg tcggtggtga tggaaataga acacatcgta ttttcatgat tgggcaaaga
    18601 ggtattttag aaatacttca ctcaagaggc gttcctttta tcatgagtga cacaggtggt
    18661 agagttaaac ctttaccaat gaggcctgat aaacttaaga atcttgggat gttaacagag
    18721 ccaggtcttt actatttata cactgatcat acagttcaaa tcgatgattt cccattacca
    18781 agagaatggc gtgatgcagg ttggttcttg gaagttaagc caccacaaac tggcggtgat
    18841 gtaattcaga tattgacgcg taatagttat gcaaggaata tgatgacttt tgaaagggtg
    18901 ctttctggaa gaactggaga catttcggac tggaattatg tgcctaaaaa tagtggtaaa
    18961 tgggagagag taccttcatt catcacaaaa atgtcagata ttaacatagt aggcatgtcg
    19021 ttttatttaa ctacggatga tacaaaacgt tttacagatt ttccaactga acgtaaaggg
    19081 gtagctggtt ggaacttata tgtagaagct tcaaacacag gtggctttgt tcataggcta
    19141 gttcgtaata gtgttacagc atctgctgag atactattga aaaattatga tagtaaaaca
    19201 agttcagggc catggacttt acacgaaggg agaattataa gttaatgagt aatttagaga
    19261 aatctgtagc tataaattta gaaaacacag cgcattatga aaatatttca aatctagata
    19321 taacttttag aacaggagag agtgattctt ctgttcttct ttttaatatc attaaaaata
    19381 atcaaccgtt attactgagt gaagaaaata tcaaagcacg aatagcgatt cgaggtaaag
    19441 gagtaatggt agttgctcca ctagaaatat tagatccatt taaaggtatt ttaaaatttc
    19501 aattacctaa tgatgtaatt aaaagagatg gaagttatca agctcaagtt tcggttgcag
    19561 aattaggtaa ttcagacgtg gtagttgtag agagaactat cacatttaac gttgaaaaaa
    19621 gtttgtttag caagattccc tctgaaacaa aactacacta tattgttgag tttcaagaat
    19681 tagaaaaaac tattatggat cgcgcgaaag caatggacga ggctataaaa aatggtgagg
    19741 attatgcgag tctgattgaa aaagctaaag aaaaaggtct atcagatatt caaatagcaa
    19801 aatcttcaag tattgatgaa ttaaagcaac ttgctaatag ccgtatatct gatttggaaa
    19861 ataaagcgca agcatattca agaacattcg atgagcaaaa gcgatatatg gatgagaaac
    19921 atgaagcctt caagcagtca gtgaatagtg gtggtttagt cacaagtggt tctacttcaa
    19981 attggcaaaa agctaagatt actaaagatg atggtaagat aatgcagatt actggatttg
    20041 attttaataa tccagaacaa agaataggtg attcaaccca atttatttat gtttcgcaag
    20101 ctataaatta tccaagagat gttagtacta acggtactgt cgaatattta gtagtaactt
    20161 cagattacaa gcgtatgact tatcgaccga acggtacaaa taaagtgttt gttaaaagaa
    20221 aagaagcggg ttcatggtct gagtggtcag aattagctat taatgattac aatacacctt
    20281 ttgaaactgt tcaaagtgcc caatcaaaag ctaatatggc cgaaagtaac gctaaattat
    20341 acgcagatga caagtttaat aaaaggtatt cggttatttt tgatggaaca gcaaatggtg
    20401 tgggctctac attgtactta aatgagagtt tagaccaatt tattttatta attttttatg
    20461 ggacttttcc aggtggtgac tttacagagt ttggcagtcc ttttggagga ggaaagattt
    20521 cattgaatcc ctcaaatctt ccagatggtg atggaaatgg tggaggtgtt tatgagtttg
    20581 gattaactaa atctagtcgt acatctttaa ctatatcaaa cgatgtctat ttcgacttag
    20641 gaagtcaaag aggctctggt gcgaacgcaa atagagggac aattaacaaa attataggag
    20701 tgagaaaata atgcaaatat tagttaacaa gcgtaatgag ataatttcat acgctatcat
    20761 tggcggcttt gaagaaggta ttgatattga aaatttacca gaaaatttct ctcaagtttt
    20821 tagacctaaa gcctttaaat attcaaatgg ggaaatagtt tttaacgaag attattcaga
    20881 agaaaaagat gacttgcatc aacagattga cagtgaagaa caaaacacag tcgcttctga
    20941 tgacatctta cgaaaaatgg ttgctagtat gcagaaacaa gttgttcaaa gtacaaagtt
    21001 atcgatgcaa gttaataagc aaaatgcact aatggcaaaa caacttgtga cacttaataa
    21061 aaaattagaa gaggttaaag gagagactga aaatgcttaa attaatttca ccaacattcg
    21121 aagatattaa aacatggtat caattgaaag aatatagtaa agaagatata gcgtggtatg
    21181 tagatatgga agttatagat aaagaggaat atgcaattat tacaggagaa aagtatccag
    21241 aaaatctaga gtcataggtt ataatcttat ggctttttaa tttgaataaa gtgggtggtg
    21301 taatgtttgg atttaccaaa cgacacgaac aagattggcg tttaacgcga ttagaagaaa
    21361 atgataagac tatgtttgaa aaattcgaca gaatagaaga cagtctgaga acgcaagaaa
    21421 aaatttatga caagttagat agaaatttcg aagaactaag gcgtgacaaa gaagaagatg
    21481 aaaaaaataa agagaaaaat gctaaaaata ttagagacat caagatgtgg attctaggat
    21541 taatagggac gattctaagt acatttgtta tagccttgtt aaaaactatt tttggcattt
    21601 aaaggaggtg atcaccatgc ttaagggaat tttaggatat agcttttggt cgtgtttctg
    21661 gtttggtaag tgtaagtaat atttaagagt cagtgcttcg gcactggctt tttattttgg
    21721 ataaaaggag caaacaaatg gatgcaaaag taataacaag atacatcgta ttgatcttag
    21781 cattagtaaa tcaattctta gcgaataaag gtataagtcc gataccagta gatgaagaaa
    21841 gtgtttcatc gattatctta acagttgttg ctttatatac tacatataaa gataatccaa
    21901 catctcaaga agggaaatgg gcgaatcaaa aattaaagaa atataaagct gaaagtaaat
    21961 atagaaaagc aacagggcaa gcgccaatta aagaagtaat gacacctacg aatatgaacg
    22021 acacaaatga tttagggtag gtgttgacca atgttgataa caaaaaacca agcagaaaaa
    22081 tggtttgata attcattagg gaagcagttc aatcctgatt tgttttatgg atttcagtgt
    22141 tatgattacg ccaatatgtt ctttatgtta gcgacaggcg aaaggctgca aggtttatat
    22201 gcttataata tcccgtttga taataaagca aagattgaaa aatatggtca aataattaaa
    22261 aactatgaca gctttttacc gcaaaagttg gatattgtcg ttttcccgtc aaagtatggt
    22321 ggtggagctg gacacgttga aattgttgag agcgcaaatt taaacacttt cacatcattt
    22381 ggtcaaaact ggaacggtaa aggttggact aatggcgttg cgcaacctgg ttggggtcct
    22441 gaaactgtga caagacatgt tcattattat gacaatccaa tgtattttat taggttaaac
    22501 ttccctaaca acttaagcgt tggcaataaa gctaaaggta ttattaagca agcgactaca
    22561 aaaaaagagg cagtaattaa acctaaaaaa attatgcttg tagccggtca tggttataac
    22621 gatcctggag cagtaggaaa cggaacaaac gaacgcgatt ttatacgtaa atatataacg
    22681 cctaatatcg ctaagtattt aagacatgca ggacatgaag ttgcattata cggtggctca
    22741 agtcaatcac aagatatgta tcaagatact gcatacggtg ttaatgtagg caataaaaaa
    22801 gattatggct tatattgggt taaatcacag gggtatgaca ttgttctaga aatacattta
    22861 gacgcagcag gagaaagcgc aagtggtggg catgttatta tctcaagtca attcaatgca
    22921 gatactattg ataaaagtat acaagatgtt attaaaaata atttaggaca aataagaggt
    22981 gtaacacctc gtaacgattt actaaatgtt aatgtatcag cagaaataaa tatcaattat
    23041 cgcttatctg aattaggttt tattactaat aaaaatgata tggattggat taagaaaaat
    23101 tacgacttgt actctaaact aatagctggt gcgattcatg gtaagccaat tggtggagtg
    23161 gtagctagtg aggtcaaagc gccagttaaa aacgaaaaga atccgccagt gccagcaggt
    23221 tatacactcg ataagaataa tgtcccttat aaaaaagaac aaggcaatta cacagtagct
    23281 aatgttaaag gtaataatgt aagagacggt tattcaacta attcaagaat tacaggggta
    23341 ttacccaaca acacaacaat tacgtatgac ggtgcatatt gtattaatgg ttatagatgg
    23401 attacttata ttgctaatag tggacaacgt cgctatattg cgaccggaga ggtagacata
    23461 gcaggcaacc gaataagcag ttttggtaag tttagtgcag tttgataatt gtatatgatg
    23521 aatcttaggc aggtacttcg gtacttgcct attatttaaa attaataaac agttaatttt
    23581 tacatgaata tattaaattt taaaaaaaca aacgttt
//
LOCUS       DAMZLE010000028        23153 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMZLE010000028 DAMZLE010000000
VERSION     DAMZLE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.028"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            141..341
                     /locus_tag="POB92_000986"
     CDS             141..341
                     /locus_tag="POB92_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDI6578496.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYINDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            364..825
                     /locus_tag="POB92_000987"
     CDS             364..825
                     /locus_tag="POB92_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578497.1"
                     /translation="MYNRKEIREMIDNYKWMKNIIDSKVYDNESTSIAQYGYQSAMPK
                     AKGTTSNKVLVKVINKNKALRKYDYLINKIAFIDEYEEYITNEKDYHILQMLKQRESH
                     NRIMSILDIGRDNFYSRVKDIVNILYNLQQETDTSDSSDTSYKSYKSYTSD"
     gene            940..1392
                     /locus_tag="POB92_000988"
     CDS             940..1392
                     /locus_tag="POB92_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HDI6578498.1"
                     /translation="MEISKYQEIATRTHNDELNLNESITCYGLGLTQSTGNVTDLIKQ
                     HMFCNVPIDKGIMINELSEALWNIANLTNVLGINLDEIAGHSVNTILMNKPNQTINLD
                     NGIKQGDKVLFQGSKYLVDGSIGNLLLISNDKDDRQVTVQDVKKVDKE"
     gene            1408..1752
                     /locus_tag="POB92_000989"
     CDS             1408..1752
                     /locus_tag="POB92_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6578499.1"
                     /translation="MKRCGHPTCNVLINHNESYCDKHKQYANENYNDLRRRNDPEYLR
                     FYKSKTWQNMRRIVLLEHDFICVSCGNQATMVDHIVPTKIDWARRLDKSNLQPLCDAC
                     HNQKTKEDLKKY"
     gene            1881..2348
                     /locus_tag="POB92_000990"
     CDS             1881..2348
                     /locus_tag="POB92_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDI6578500.1"
                     /translation="MAGRPKKLLSNSNKNYTKEEIIEKERQEAQLNKFSKIDTEPPHF
                     LDEIAKQEYLRILPHMQELPISNLDKAQLAQYCSFYSDFVKASLILEREDLILEDDKG
                     NQKVNPAFNIKEKAGIRLQQTANTLGLTIDSRLRIMVPDEKEDDDPYMEFVSD"
     gene            2351..4045
                     /locus_tag="POB92_000991"
     CDS             2351..4045
                     /locus_tag="POB92_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDI6578501.1"
                     /translation="MTDYVTKYAKKVVSGEILASLKNIQVCKRHLSFMENPPNGCHWD
                     NHLSNKAIKFVEMLPDPKTNQPMPLMEFQKFIVGSLYGWRRGQYRMFTKAYISMARKQ
                     GKSLIVSGMSVNELLFGQYPKFNRQIYVASSTYKQAQTIFKMASQQVNLMRSKSKFIR
                     EKTDVRKTDIEDVLSSSVFAPLSNNPDAVDGKDPTVAILDELASMPDDEMYSRFKTGM
                     TLQKNPLTLLVSTAGDNLNSQMYQEYKYIKRILNEEVRADNYFVYCAEMDSQEEVQDE
                     TKWIKAMPLLESKEHRKTILQNVKADIQDELEKGTSYHKILIKNFNLWQAQREDSLLD
                     ISDWEQVITPMPNINGKDVYIGVDLSRLDDLTSVGFIFPNDDKKVFLHSHSFIGLRTN
                     LEQKSKRDKINYELAIERGEAETTQSDSGMIDYKQVIDFIVKFITTHDLNVQAVCYDP
                     WNAQSFITTIESMALDWPLIEVGQSFKALSQSIKEFRMWVADERIQHNDNMLLTTSVN
                     NAVLIRDGEDNVKINKKMNRQKIDPIISIITAFTEARMHEFQENWTEKYESEEFGF"
     gene            4059..4259
                     /locus_tag="POB92_000992"
     CDS             4059..4259
                     /locus_tag="POB92_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578502.1"
                     /translation="MDLNKINVFFNFLVANLVSILFLLGLFVVNVSMYKAFGQNIGLL
                     CIGITLIVISLILNHESNQERS"
     gene            4265..5515
                     /locus_tag="POB92_000993"
     CDS             4265..5515
                     /locus_tag="POB92_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6578503.1"
                     /translation="MGIFYKNEKRDLQYNEDDLQMMVQTLPGFQGTKLRQYKDIEAIR
                     HSDIFTAVMMIASDLARMPIRVTVNGQINYSDRIVNLLNTRPNPMYNGYIFKLVVFVS
                     ALLTSHGYIEITRDKTGEPMNLTFRKTSEIELKSDARGRLYYFHQRIDSNGNNIERNV
                     KFEDMLDIKFYSLDGINGLSLLDTLSRTIESDNNGKDFLNNFLRNGTHAGGILKMKGV
                     LDNKKARDRAREEFHKSFSGTKQAGKVVVLDESMTFDQLEVDTEVLKLIRENKSSTRE
                     IAGVFGIPLHKFGIETANMSITDANLDYLSTLKPYITCVCAELNFKFNDEYVNREFKF
                     DTTEIRVVDEKTQAEIDKINIDSGKMNIDEIRQRDGLAPIPGGNGSIHRVDLNHVNIE
                     LVDEYQMNKSRATDKKLKGGEENE"
     gene            5508..6092
                     /locus_tag="POB92_000994"
     CDS             5508..6092
                     /locus_tag="POB92_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDI6578504.1"
                     /translation="MSKETRVGNIIEVRSNDNNEMVIEGYALKFDTWSENLGGFKETI
                     SRRALENTDLSDVRCLVDHIPSQIIGRTKSGTLELETDDVGLKYRCKLPNTTFARDLY
                     ENMRVGNINQCSFGFMLDDKGDEVRFDEQENIYKRTLTAIRELTDVSVVTYPAYKDTD
                     VKPALRSIETVKKEQRKKELEIRLKKHSILNNIW"
     gene            6180..7427
                     /locus_tag="POB92_000995"
     CDS             6180..7427
                     /locus_tag="POB92_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6578505.1"
                     /translation="MKTKEELQSEISDIKRQIDLKVKYATRALNNDELEKAEKLEQEI
                     TDLRSQIQEKQEELDKLKEKDGTSENNQQSVEVNEARTYRNQANINDLGISIQNTKVT
                     SQEVRDFTEYLETRNDIQGGSLKTDSGFVVIPEEIVTDILKLKEVEFNLDKYVTVKRV
                     TNGSGKYPVVRQSEVAALEKVEELEENPELAVKPFFQLAYDINTHRGYFRISREAIED
                     AKVNVLQELKLWMARTIAATRNKAIIDVITKGSTGSTSSGFEKEGKKLEVKKAKSLDD
                     IKDAINLNVKPNYEHNVAIVSQTMFAKLDKMKDKLGNYLIQPDVKEKTQQRLLGAKIE
                     ILPDEVLGQKGNNTLIIGNLKDAIVLFDRSQYQASWTDYMHFGECLMIAVRQDCRILD
                     YKSAIVIEYDDSERGEGDLGLEA"
     gene            7463..7621
                     /locus_tag="POB92_000996"
     CDS             7463..7621
                     /locus_tag="POB92_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578506.1"
                     /translation="MAMYEVKKSYTDLEKGQYLKSGKRVEMTVKRAEYVNKKLKEHGV
                     ILERVKEE"
     gene            7630..7962
                     /locus_tag="POB92_000997"
     CDS             7630..7962
                     /locus_tag="POB92_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDI6578507.1"
                     /translation="MQLTAEELKLLKKHCKIDHNSEDDLLEIYYSWAFHEIASAVTDE
                     PSKYIDWFKSHPLFARAIYPLASYYFENRIAYLDRDLSLAPHMVLSTVHKLRGSFEQF
                     LESENDEI"
     gene            7949..8284
                     /locus_tag="POB92_000998"
     CDS             7949..8284
                     /locus_tag="POB92_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDI6578508.1"
                     /translation="MMKFNSNKLNERIDFCEDVSERVNGNPMKPKTKILYSCFACIQE
                     SKESDTQTNLNTGSKFIKTIIIRDTRGDYKPTNKHYVLHEGQRFNIKYVKPDYQDKSY
                     LRIYGEVVI"
     gene            8284..8661
                     /locus_tag="POB92_000999"
     CDS             8284..8661
                     /locus_tag="POB92_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDI6578509.1"
                     /translation="MGARIESNNIEQGLKNAVLKMNLNSNVIVKAGAMSLVPLLKSNT
                     PFANTKKHARDHIAVSNVKTDRHTSEKIVTIGYAKGVSHRIHATEFGTMYQKPQLFIT
                     KTEKQGKNKVLKTMLDTAKRLQK"
     gene            8658..9038
                     /locus_tag="POB92_001000"
     CDS             8658..9038
                     /locus_tag="POB92_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578510.1"
                     /translation="MINVTKLIRNAIIANNITDEVNVFNYTIDDHFHEKTDKPIIRIY
                     PLPFNPDTYADDNEISREYHYQIDVWWSQDEPNEQVEKIVELLKVINFQCYYREPLYE
                     SDVMSFRHIIRAKGSILSMKLEEN"
     gene            9039..9992
                     /locus_tag="POB92_001001"
     CDS             9039..9992
                     /locus_tag="POB92_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDI6578511.1"
                     /translation="MIEKLKQAPRFLKLNLQHFADTGVSGIAIGVSNFYYAPILKDTE
                     NEWETGAGTRIRFLKEIEVDRPQDTEEDYGDDMVAATAVSNGKLSVKTTFVTVPADDK
                     AFLNGAKKGVGGYKYGAKDIPPDVAIVFERRNHDESSEWVGLFKGKFTRSSIKGQTKQ
                     DKVEFQNDDVEGNFIDRLFDESSHVTGYDKKGSTTGRDYVFMETFGKTYDEFMSSRGE
                     QNMEPVEKEMKKTEKVEVTSVNVTDEQVTVKVDATKQLSATTEPSGQKVTYAVTEGQT
                     YASVTSTGLVKGLAEGNATVTATAGKQTDTVQITVQSNLEM"
     gene            10057..10503
                     /locus_tag="POB92_001002"
     CDS             10057..10503
                     /locus_tag="POB92_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578512.1"
                     /translation="MERTSIELITGFTKTGKPQYQKYLAKPIITLFETIQGSKLGLKL
                     NKAFKGADFKDLTEEEFNNLSVTEQEEYKNKQEEYENNMAVQMEVLEEVLDFIVEAFD
                     NQFTSIELQKGLPNGQEGIEKIGQLIGRITGGEPSDTKKFVTENQK"
     gene            10563..10685
                     /locus_tag="POB92_001003"
     CDS             10563..10685
                     /locus_tag="POB92_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578513.1"
                     /translation="MIEKGMDAEKVANMPIHFFLDIVESKIETKRTAKSFKDIF"
     gene            10741..15390
                     /locus_tag="POB92_001004"
     CDS             10741..15390
                     /locus_tag="POB92_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6578514.1"
                     /translation="MPNPIGNMVIKVDLDGSGFNRGVTGLNRQMKMVSRELSANLSQF
                     SRYDNSLEKSKIKVEGLSKKQKVQAQITKELKDSYDKLSKETGENSAKTQAAAAKYNE
                     AYAKLNQYERELNQATQELKDMQREQKALNTAMGKLGTNFNNFGPKLQEIGNSMKNVG
                     RNMTMYVTAPVVAGFAVAAKKGIEFDDSMRKVKATSGATGEEFEALKKKAREMGATTK
                     FSASDSAEALNYMALAGWDSKQMMEGLSGVMDLAAASGEELGAVSDIVTDGLTAFGLK
                     AKDSGHFADVLAQTSSKANTDVRGLGEAFKYVAPVAGALGYTIEDTSIAIGLMSNAGI
                     KGEKAGTALRTMFTNLSSPTRAMGNEMERLGISITDSNGKMIPMRKLLDQLREKFKHL
                     SKDQQASSAATIFGKEAMSGALAIINASDEDYQKLTKSIDSSTGASKRMADTMESGLG
                     GKLRTLRSQLEELALTIYDRIEPALKIIVSAFSKVVTWVTKLPTSIQLAVVGFGLFAA
                     VLGPLVFMFGLFISVMGNAMTVLGPLLINVNKAGSIFAFLRTKIASLVKLFPILGVSI
                     SSLTLPITLIVGALVGIGIAFYQAYKRSETFRNIVNQAISGVANAFKAAKLALQGFFD
                     LFKGDSKGAVTLEKIFPPETVAGIQNVVNTIRTTFFKVVDAIVGFAKEIGAQLASFWK
                     ENGSEITQALQNIAGFIKATFEFIFNFIIKPIMFAIWQVMQFIWPAVKALIVSTWENI
                     KGVIQGAINIILGIIKVFSSLFTGNWRGVWDGIVMILKGTVQLIWNLIQLCFVGKILG
                     VVRYFGGLLKGLITIIWVAIIGVFKKSLSAIWNATKSIFGFLFNSVKSIFTNMKNWLS
                     STWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWNSIKDNTVGIA
                     GRLWDRVRNIFGSMRDGLKSIIGKIKDHIGGMVDAVKRGLNKLIEGLNWVGGKLGMDK
                     IPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMIEFPNGKRVLT
                     PNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSIGTMWKDIKSGASSAFNWTKDQIGK
                     GTKWLGDKVGDVMDFIDNPGKLLNYVLQAFGVDFSSLTKGMGIAGDITKAAWSKIKKS
                     AIKWLEDAFAESGDGGVLDMNKLRYLYGHTAAYTRETGRPFHEGLDFDYIYEPVPSTI
                     NGRAQVMPFHNGGYGKWVKIVKGALEVIYAHLSKYKVKTGQQVRVGQTVGISGNTGFS
                     TGPHLHYEMRWNGRHRDPLPWLRKNNGGGKSTPGGNGAANARRAIKAAQNILGGRYKA
                     SWITNEMMRVASRESNYTANAVNNWDSNARAGIPSRGMFQMIDPSFRAYAKSGYNNPL
                     NPTHQAISAMRYIVGKWVPRTGSWRAAFKRAGDYAYATGGKVYNGLYHLGEEGYPEWI
                     IPTDPSRANEAHKLLALAANDIDNRSKNKRPNNLPNPSISNSDTNYIHTLENKLDAVI
                     NCLVSLVESNQVIADKDYEPVINKYVFEDEVNNSIDKRERHESTRVRFRRGGTII"
     gene            15390..16880
                     /locus_tag="POB92_001005"
     CDS             15390..16880
                     /locus_tag="POB92_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6578515.1"
                     /translation="MQDTIQIDNKTIEWLVVQRGFEIPSFNFVTEKESVKGRTGSIAK
                     ARYLNDIEFELPLIIRNEVLAPGGQKTHDDILEELVEFFDIDNLKPKKLKFKSQNWYW
                     FAYFDGPLKLPKNPRGSVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPL
                     IVEARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITE
                     DIPSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCI
                     VEQKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQ
                     NKPIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQ
                     RPASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSV
                     VINEEPMLSEKSFGSTYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            16896..20678
                     /locus_tag="POB92_001006"
     CDS             16896..20678
                     /locus_tag="POB92_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578516.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKITSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDSELEYFERKIHKSDT
                     PPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEETPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRKTLTRYGSQIIQNGKEIKLRTTKEEFNASKRTLSRVLADITVNA
                     MKGIYLRYDENGAITSHTIDKDGVKISGDKVDITANREFNVVANNINNKVGKNDIVNS
                     LNLSNEGLDINVNRIGIKGGNANRYVQVQNDFIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDYNRIIIDSYASANIESREAPIYLSPNTKNKPGLNRFAFTLSNADSAYET
                     DGYIMFGSDENYKYGAGLRFSKRSNKGLVQVVNGDYATGGDTTIESGMGKFNIVKRRD
                     GNSYVSIQSYDLLAVGSDNAGDRVASNSIYKRTYSAPANLHITSAGTIGRATSAKKYK
                     ISIENQYINEDDQFSHSKEILKLPIRTWFDKYESEIMAKELESGKKLSDDTFKLSRHT
                     GLIAEEVEELGFNEFVIYDDNGEIEGIAYDRLWVHLIPIIKNQQSKIEKLEELINE"
     gene            20671..20823
                     /locus_tag="POB92_001007"
     CDS             20671..20823
                     /locus_tag="POB92_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578517.1"
                     /translation="MNDSNQGLQANPQYTIHYLSQEITRLTQENAMLKAYIQEQNEKS
                     KSAEEE"
     gene            20869..21156
                     /locus_tag="POB92_001008"
     CDS             20869..21156
                     /locus_tag="POB92_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578518.1"
                     /translation="MANEIIKKTERFILVQIDKEGTERVLYQDFVGSFTTSDSASYAQ
                     DFKSEENAKKIAETLNLLYQLTGNQNGVKVVKEVVDRTDLSSDKSVDSEIM"
     gene            21212..21586
                     /locus_tag="POB92_001009"
     CDS             21212..21586
                     /locus_tag="POB92_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578519.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
     gene            21729..21905
                     /locus_tag="POB92_001010"
     CDS             21729..21905
                     /locus_tag="POB92_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDI6578520.1"
                     /translation="MDRWWLSEYKEVVPMLALLKSLERRCLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(21958..22065)
                     /locus_tag="POB92_001011"
     CDS             complement(21958..22065)
                     /locus_tag="POB92_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578521.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            22117..22371
                     /locus_tag="POB92_001012"
     CDS             22117..22371
                     /locus_tag="POB92_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6578522.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            22383..23138
                     /locus_tag="POB92_001013"
     CDS             22383..23138
                     /locus_tag="POB92_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6578523.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT         8532 a         3264 c         4748 g         6609 t
ORIGIN      
        1 tcagagtacc gacgaaaaat aaacgattat aatctttatc ttgataaatt tgaagaacag
       61 tttagtcaaa agtttaaaat aaacagaact tcgataaaag aaagaattat tattaattta
      121 aacaagagga gatttaaatg atgtggatta ctatgactat tgtatttgct atattgctat
      181 tagtttgtat cagtattaat agtgatcgtg caagggagat acaagcgctc agatatataa
      241 atgattatct acttgatgaa gtagttaaaa ctaaaggata caacgggtta aaagaataca
      301 ggattgaatt aaagcgaatg aataacgata ttaaaaagta atttatatta tcggaggtat
      361 tgcatgtata acaggaaaga aatacgtgaa atgatagata actacaagtg gatgaaaaat
      421 ataatagaca gtaaagtcta cgataacgaa agtacatcaa ttgcacaata cggttatcaa
      481 tctgcgatgc caaaagctaa aggcacgact agcaataaag tgttagtgaa agttataaac
      541 aaaaacaaag cgcttagaaa gtacgattac ttgattaata agatagcgtt cattgatgaa
      601 tacgaagaat acatcacgaa tgaaaaagat tatcatattt tacaaatgtt aaaacaacga
      661 gaaagccata ataggattat gagtattctt gatataggca gagacaattt ttattctaga
      721 gtaaaagata tagtaaatat actttataac ttgcaacaag aaaccgacac atcggacagt
      781 tcggacacat cgtacaaatc gtacaaatcg tacacatcgg actaattttg atgctacata
      841 ttgtttttta ttataattgc tatgtagcaa aacatttata tttattttga actctcacat
      901 taagtgaggg tttttatttt tataaacaag aggtggagaa tggagatatc aaagtaccaa
      961 gagatagcta cacgtacaca caatgatgaa ttgaatttaa atgaatctat tacttgttac
     1021 ggcttaggtt taactcaatc tacaggcaat gttacagatc taattaaaca gcatatgttt
     1081 tgtaatgtac cgatagataa aggaattatg ataaatgaac ttagcgaagc attgtggaat
     1141 atagctaatc ttactaacgt gttaggtatt aacttggatg agatagctgg tcatagtgtt
     1201 aacactatct tgatgaataa acctaatcag actatcaatt tagacaatgg tataaaacaa
     1261 ggagacaaag tattgtttca aggtagtaaa tatcttgttg atggatcgat aggaaactta
     1321 ttgttaatta gcaatgataa agatgataga caagtaactg tgcaagatgt taagaaagtc
     1381 gacaaggagt gatgtgcatt gtctattatg aagcgatgtg gtcatccaac atgtaatgta
     1441 ttgattaatc ataatgaaag ttattgtgat aaacacaagc aatatgcaaa tgaaaattac
     1501 aatgatttga gacgtcgaaa cgatccagag tatttaagat tttataaatc gaaaacgtgg
     1561 caaaacatgc gtcgaattgt attgttagaa catgatttta tttgtgtttc ttgtggcaat
     1621 caagcgacta tggttgacca tattgtacca acaaaaattg attgggcaag aagattagac
     1681 aaaagtaatt tacagccttt gtgtgatgct tgccataacc aaaagacaaa agaagatttg
     1741 aagaaatatt aaaaaagata aaaataggaa gtcccccaaa gatgaaacgg gcgtcaatga
     1801 aaggttctgg agaacggagc agagttttct tctcaaaaaa ttccctttat ttaagttttt
     1861 ttagtaggag gtgctaattt atggcgggta gacctaagaa gcttttgtca aattcgaaca
     1921 agaattatac aaaagaagaa attattgaaa aagagcgtca agaagctcaa ttaaataaat
     1981 tttctaaaat cgatactgaa ccaccgcact ttttagatga aatagcgaaa caagaatact
     2041 taagaatatt accgcacatg caagaattgc caatttccaa cttagataaa gcacaattag
     2101 cacaatattg tagtttttat agtgactttg ttaaagcaag tttgatttta gagcgcgaag
     2161 acttgatttt agaagacgac aaaggaaatc aaaaggttaa tccggcgttc aacataaagg
     2221 aaaaagcggg tattcgattg caacaaacag ctaatacttt aggattaact attgatagcc
     2281 gattgcgtat tatggttcct gatgaaaaag aagatgatga tccatatatg gaatttgtga
     2341 gtgattagta atgactgatt atgttactaa atacgcaaaa aaggtagttt caggagaaat
     2401 tttggcaagt ttgaagaata ttcaagtatg caaacgtcac ctatctttta tggagaaccc
     2461 gccgaatggt tgccattggg ataatcattt gtctaacaaa gcaattaaat ttgtggaaat
     2521 gcttccagac cctaaaacaa accagcccat gcctcttatg gagtttcaga aattcattgt
     2581 tgggagctta tacggctggc gtagaggtca atacagaatg tttactaaag cttatataag
     2641 tatggctaga aagcaaggta agtctctaat cgtatcggga atgtccgtta acgaactgtt
     2701 gtttggacaa taccctaaat ttaatagaca aatttatgta gcttcatcta cttataagca
     2761 agcgcaaaca atattcaaga tggcaagcca acaagtaaac ctaatgcgaa gtaaaagcaa
     2821 gtttatccgt gaaaaaacag acgtaagaaa gacagacatt gaagatgtat taagtagttc
     2881 agtgtttgca cctctttcca ataacccaga tgcggttgat ggtaaagatc ctacagttgc
     2941 tattttggac gaattggcaa gtatgcctga tgatgagatg tactcaaggt ttaaaacagg
     3001 tatgacatta caaaaaaatc ctttaaccct acttgtttca acggccggag acaatttaaa
     3061 tagtcaaatg taccaagagt ataagtatat taaacgtatt ttaaatgaag aagtaagagc
     3121 tgataattac tttgtatatt gtgctgaaat ggattcacaa gaagaagttc aagatgaaac
     3181 aaagtggatt aaagcaatgc cgcttttaga atcaaaagaa catagaaaaa ctatacttca
     3241 aaatgtaaaa gctgatatac aagacgaatt agaaaaaggg acatcgtatc ataagatttt
     3301 gattaaaaac ttcaatttat ggcaagcgca aagagaagat agcttgctag atatttcaga
     3361 ttgggaacaa gtaataacgc ctatgcctaa tatcaatggt aaagatgtgt atataggtgt
     3421 cgacttatcg agattggatg acttaacatc tgtagggttt attttcccta acgacgataa
     3481 aaaagtgttt ttacatagtc attctttcat tggattaaga acaaacttag aacaaaaatc
     3541 taagagagac aaaataaatt atgaattagc gattgaacgt ggagaagctg agactacaca
     3601 atcagatagc ggcatgattg attataaaca agttatcgat tttatagtga aatttataac
     3661 gacgcatgac ctgaatgtac aggctgtttg ctatgaccct tggaatgcgc aaagttttat
     3721 aacaacaatc gaatcaatgg ctttagattg gccactcatt gaagtgggac aaagttttaa
     3781 ggcgttatca caatctatta aagaatttag aatgtgggtt gcagatgaaa gaatacagca
     3841 taacgataat atgttactta caacatcagt taataatgcc gttttgattc gtgacggaga
     3901 agacaatgtg aaaataaata aaaaaatgaa tcgtcaaaaa atagatccga ttatttcgat
     3961 tatcacagct ttcactgaag ctagaatgca cgaattccaa gaaaattgga cggagaaata
     4021 tgaaagcgaa gaattcggat tttaaaggtg gtgacaaaat ggacttgaat aaaataaatg
     4081 tcttttttaa tttcttggtt gctaatttgg ttagcatcct ttttttatta ggtttgtttg
     4141 tggttaatgt ttctatgtat aaagcattcg gtcaaaatat aggactttta tgcattggta
     4201 taacactgat tgttatttcg ttgattttaa atcatgaaag caatcaagaa aggagttagt
     4261 agttgtgggg attttttata aaaatgaaaa acgagacttg caatacaacg aagatgattt
     4321 gcaaatgatg gttcaaactt tgccaggttt tcaaggaaca aaattacgac aatataaaga
     4381 tatagaagca attaggcata gcgacatctt tactgcagtt atgatgattg cttctgattt
     4441 ggcgcgcatg ccaattaggg tgacagtgaa cggccaaatt aattatagtg acaggattgt
     4501 taatttgtta aatacacgtc ctaacccaat gtataacggc tatatattca aattagtagt
     4561 gtttgttagt gccttactaa catcgcacgg ctatattgaa attacacgtg ataaaacagg
     4621 agaacctatg aatttaacgt tcagaaagac atccgaaata gaattgaaat cagacgcaag
     4681 aggtcgactg tattattttc atcaaaggat agacagtaac ggaaataata tagaacgtaa
     4741 tgttaagttt gaggatatgc tagacatcaa attttattcg ttggatggta taaatggttt
     4801 gtcactgtta gacacattaa gtcgcacgat agaatcagat aacaatggaa aagatttcct
     4861 taataatttc ttgcgaaatg gcacacatgc tggtggtatt ttgaaaatga aaggtgtatt
     4921 agataataaa aaagcaagag accgtgccag agaagaattt cacaaaagtt ttagtggaac
     4981 taaacaagct gggaaagttg tcgtactcga tgaatcaatg acgtttgatc aattagaagt
     5041 tgatacagaa gttttaaagc ttatcagaga aaacaaatca tcaacaagag aaatagcagg
     5101 tgtatttggt attccattgc ataagttcgg catagaaaca gcgaacatga gtatcacgga
     5161 tgctaattta gattacttat caactttaaa accttatatt acatgcgttt gtgcagaatt
     5221 gaattttaag tttaatgatg aatatgtgaa tcgtgaattt aaatttgata ccactgaaat
     5281 acgagttgtt gatgaaaaaa cacaagctga aattgacaaa attaacattg attctggaaa
     5341 gatgaatatc gatgaaatta gacaacgtga tggattagcg ccaataccag gcggtaatgg
     5401 tagcattcac agagtcgatt taaaccatgt aaatattgaa cttgtagatg agtatcagat
     5461 gaataaatcg agagctactg ataaaaaatt gaaaggtggt gaggaaaatg agtaaggaaa
     5521 cgagagttgg caacattatt gaggtacgct caaatgataa caacgaaatg gtcatagagg
     5581 ggtatgcgtt aaagtttgac acttggtctg aaaatcttgg tggattcaaa gaaacgattt
     5641 cacgtcgcgc tttagaaaac actgatttat ctgatgtacg ttgtttagta gatcatatcc
     5701 catcgcaaat aattggtagg acaaaatcgg gtactttgga gctcgaaact gatgatgttg
     5761 gacttaaata tcgttgtaag ttaccaaaca caacatttgc acgtgattta tatgagaaca
     5821 tgcgtgtagg caacatcaat caatgttcgt ttggttttat gcttgacgat aaaggcgatg
     5881 aagtgcgttt tgatgaacaa gaaaacattt acaaacgtac tttaacagca attcgtgaac
     5941 ttacagatgt ttctgtagtg acttatccgg cttacaaaga cactgatgtt aaaccagcat
     6001 tacgtagtat tgaaaccgtt aaaaaagaac aacgtaaaaa agaattagaa ataagactaa
     6061 agaaacactc tatattaaat aatatttggt gaagttgaac accattatca aatacagcca
     6121 ttggacatgc tgaatatagc gatgtctatt tttttatgcc aattttagga ggaaattaaa
     6181 tgaaaacaaa agaagagtta caatctgaga tttcagacat taaaagacaa attgatttaa
     6241 aggtgaagta tgcaacgaga gcacttaata acgatgagtt agaaaaagca gaaaaattag
     6301 aacaagaaat tactgattta cgttctcaaa tccaagaaaa acaagaagaa ttagataagc
     6361 taaaagaaaa agatggaact tcagaaaaca atcaacaatc agtggaagta aacgaagcac
     6421 gtacttatcg aaaccaagca aacattaatg atttaggtat ttcgattcaa aacacaaagg
     6481 taacatcaca agaagttaga gattttactg aatatcttga aacacgcaat gatattcaag
     6541 gtggttcgtt aaaaacagac tcaggatttg tagttattcc agaggaaatt gttacagata
     6601 ttttaaaatt aaaagaggtt gagtttaatc ttgataagta tgtgacggtc aaacgtgtta
     6661 caaatggttc tggtaaatat ccggtagtac gacaatcaga agttgcagcc cttgaaaaag
     6721 ttgaagaatt agaagaaaac cctgaattag cagttaaacc attcttccaa ttagcatatg
     6781 acattaatac acaccgtggt tacttccgaa tttcacgtga agcaatcgaa gatgcaaaag
     6841 tgaatgtttt gcaagaattg aaactatgga tggcgcgaac tattgcagca acacgaaaca
     6901 aagcaattat tgatgttatc actaaaggat caacgggttc tacaagttca ggttttgaaa
     6961 aagaaggtaa gaaattagaa gttaaaaaag caaaatcttt agatgatatt aaagatgcta
     7021 ttaacctgaa tgttaagcca aattacgaac ataatgttgc gattgtttcg caaactatgt
     7081 ttgcaaaatt agacaaaatg aaagataagc taggaaacta tttaatccag ccagatgtta
     7141 aagaaaaaac gcaacagcgt ttattaggag ctaaaatcga aattttacct gatgaagtac
     7201 tagggcaaaa aggtaataac actttgatta tcggtaactt aaaagatgcg attgttttat
     7261 ttgaccgctc tcaataccaa gcatcatgga ctgactacat gcatttcgga gaatgtttaa
     7321 tgattgctgt acgtcaagac tgtagaattc tagattataa atcagcaatt gtgattgaat
     7381 atgatgatag tgaacgcggt gaaggcgatc ttggcttaga agcataataa gcgctcgata
     7441 ctttataaag aggtgataaa ctatggcaat gtatgaagtg aagaaatctt atactgactt
     7501 ggaaaaaggc cagtatttaa agtcaggtaa acgtgttgaa atgacagtaa aacgtgctga
     7561 atatgttaac aaaaagctga aagagcatgg agtaatactt gaaagagtaa aagaagaata
     7621 ggtgattgaa tgcaattaac agctgaggaa cttaagttat taaaaaagca ttgcaaaata
     7681 gatcacaatt cagaggacga cttattagaa atatattact cttgggcatt ccatgaaata
     7741 gctagcgctg ttacggatga accaagtaaa tatattgatt ggtttaaaag tcatcctcta
     7801 tttgctcgtg ctatataccc tttagcaagt tactattttg aaaaccgtat tgcttatttg
     7861 gatagggatt tatcgcttgc gccacatatg gttttaagta cggtgcataa attgagaggt
     7921 tcatttgagc aatttttgga gagtgaaaat gatgaaattt aattccaata aattaaatga
     7981 acgtatagat ttttgtgaag atgtaagcga gagagtgaac ggaaatccga tgaaaccgaa
     8041 gacgaaaata ttatactctt gtttcgcttg cattcaagaa tctaaagaat ccgacactca
     8101 aacgaatctc aatacaggta gcaaattcat taaaactatt attatcagag atacacgagg
     8161 tgattataaa ccaacaaata agcattacgt cttgcatgaa gggcaaagat ttaacatcaa
     8221 atatgtaaag ccagattatc aagataaatc ttatttgcgt atctatggcg aggtggtcat
     8281 ttaatggggg caagaattga aagtaataac atcgaacaag gtttgaaaaa tgcagtttta
     8341 aaaatgaatt taaatagtaa tgtaattgtc aaagctgggg ctatgtcatt agtcccgctt
     8401 ttaaaaagta atacaccttt tgcgaatact aaaaagcatg ctcgcgatca catagctgtt
     8461 tctaatgtga aaacagacag acacacaagt gagaaaatcg ttacaattgg ttacgctaaa
     8521 ggcgtctcac atcgtattca tgcaacagaa tttggaacaa tgtaccaaaa accacaattg
     8581 tttataacaa aaacagaaaa gcaagggaaa aacaaagttt taaaaacaat gcttgatact
     8641 gctaagaggt tgcaaaaatg attaatgtta ccaaattaat tagaaacgct attattgcaa
     8701 ataacattac agatgaagtg aatgtgttta actacactat agatgaccat tttcacgaaa
     8761 aaactgacaa gcctattatt cgcatatatc ccttaccgtt caatcctgac acatacgctg
     8821 atgataacga gatttcaaga gaataccatt accaaattga tgtttggtgg tctcaagatg
     8881 aaccgaacga gcaagtagaa aaaattgttg agttactcaa agtgataaat tttcaatgtt
     8941 attacagaga accgttatac gagagtgacg tcatgtcatt cagacatatt ataagagcaa
     9001 aaggctcgat tttatcaatg aaattggagg aaaattaaat gattgaaaaa ttgaaacaag
     9061 caccaagatt tttaaaatta aacttacaac attttgcaga tacaggagtt tcgggtatcg
     9121 caattggggt atcaaacttt tattatgcac ctattttaaa agatacagaa aatgaatggg
     9181 aaactggagc tggcacacgt attcgtttct taaaagaaat tgaagtagac cgtccacaag
     9241 ataccgagga agattatggg gatgatatgg tcgcagcaac tgctgtatct aatggtaaac
     9301 taagtgttaa gacaacattt gttactgttc ctgctgacga taaggcgttc ttgaatggcg
     9361 ctaaaaaagg tgtaggtggt tataaatatg gagctaagga tatcccgcca gatgtagcga
     9421 ttgtatttga aagacgtaat catgatgagt cttcagaatg ggttggcttg ttcaaaggta
     9481 aattcactcg ttcaagcatc aaagggcaaa caaaacaaga taaagttgaa ttccagaatg
     9541 acgacgtaga aggcaatttt attgatcgtt tgtttgatga gagctcgcat gttactggct
     9601 atgataaaaa aggaagcact acagggcgcg attatgtatt catggaaaca tttggtaaaa
     9661 cttatgatga attcatgtct agtcgaggag aacaaaatat ggaacctgta gaaaaagaaa
     9721 tgaaaaaaac agaaaaagtt gaagtcactt ctgtaaacgt cactgatgaa caagttacag
     9781 ttaaagttga tgctactaaa caactatcag ccacaaccga accatctgga cagaaagtaa
     9841 cttatgcagt gactgagggg caaacgtatg ctagcgtaac atcaactggc ctcgttaaag
     9901 gtttggcgga aggtaatgcg accgttacag cgactgcagg aaagcaaact gatactgtgc
     9961 aaattacagt acaatctaat ttagaaatgt aagttttgag ggcttaacgc cctcttttta
    10021 ttttggccaa attaaaaaga aagtaggaat ttaataatgg aacgtacatc aattgaatta
    10081 attacaggat ttacaaaaac aggaaagccg caatatcaaa agtatttagc gaagccgatt
    10141 attactttgt ttgaaacaat tcaaggttca aaattaggtt tgaaacttaa caaagccttt
    10201 aagggggctg attttaaaga tctaacagaa gaagaattta ataacttaag tgtgacagaa
    10261 caggaagaat acaaaaacaa gcaagaagaa tacgaaaaca acatggctgt acaaatggaa
    10321 gtattagaag aagttttgga tttcatcgtt gaagcttttg ataatcaatt taccagtata
    10381 gaacttcaaa aaggattacc aaatggtcaa gaaggtattg aaaagattgg acagttaatt
    10441 ggacgaatta caggtgggga acctagcgat acaaaaaagt tcgtgacaga gaatcagaaa
    10501 taagaaaaga agatttaaca cctgaagctg tctacaacaa ttacaggaaa atagctaaag
    10561 atttgataga aaaaggcatg gatgcagaaa aagtggctaa catgccgata cacttctttt
    10621 tagacattgt cgaatcgaag attgaaacaa agcgaactgc gaaaagtttt aaagatattt
    10681 tttaatcagc ctttaaaggt tgatttttta tttacatctt ggaagaaagg aggtttttaa
    10741 atgcctaatc ctataggtaa tatggtcata aaggttgatt tagatggttc tggattcaat
    10801 agaggtgtga caggtttaaa taggcaaatg aaaatggttt cgcgtgagct ttcggctaat
    10861 ttatcacaat tttctagata tgataattca ttagaaaagt cgaagataaa agtcgaaggt
    10921 ttgagtaaaa aacaaaaagt tcaagcccag attactaaag agctgaaaga tagttatgac
    10981 aaacttagta aagaaactgg tgaaaacagt gcaaagacac aagctgcggc tgctaaatac
    11041 aatgaagctt acgctaaatt aaaccaatat gagcgagagt taaaccaagc cacacaagaa
    11101 ttaaaagaca tgcaaagaga gcagaaagca ttaaatactg caatgggaaa acttggtacc
    11161 aactttaata attttggtcc taaacttcaa gaaattggta acagtatgaa aaatgtaggc
    11221 cgtaacatga ctatgtatgt aactgcgccg gtggttgctg ggtttgctgt agcagctaaa
    11281 aaaggtattg aattcgatga cagtatgaga aaagttaaag caacttcagg tgctactggg
    11341 gaagagtttg aagctttgaa gaaaaaggct cgcgaaatgg gtgcaacaac aaaatttagt
    11401 gcatcagatt cggctgaagc attaaattac atggcacttg ctggttggga ttctaagcaa
    11461 atgatggaag gtttaagcgg agttatggat ttagcggcag catctggcga agaactggga
    11521 gcagtaagtg acattgttac agatggacta acggcatttg gtttaaaagc aaaggatagt
    11581 ggtcattttg cggacgtttt agcacaaact agctcgaagg caaatacgga tgttagaggg
    11641 ctcggagaag cttttaaata tgtcgctcct gtagcaggtg cgttaggtta cacgattgaa
    11701 gatacatcta ttgcgatagg tttaatgagt aatgctggta tcaaaggtga aaaagcaggt
    11761 acagcgttac gaacaatgtt caccaatctt tcaagtccga ctagagctat ggggaatgaa
    11821 atggagcgct taggaatatc tattacagat agtaatggga aaatgattcc tatgcgaaag
    11881 cttttagacc aactgagaga aaaatttaaa catctttcaa aagaccaaca agctagttct
    11941 gcagctacaa tatttggtaa agaagcgatg tcaggagcat tagcaattat aaatgcttct
    12001 gatgaagact atcaaaagtt aaccaaatct atagattcat ctaccggggc atctaaaaga
    12061 atggccgata caatggaatc tggtttaggt gggaaattaa gaactttaag gtcgcaatta
    12121 gaagaactag ccttaacgat ttatgacaga atagaaccag cactaaagat tatagtaagt
    12181 gcttttagca aagtagtgac atgggttact aaattaccaa cgtcaattca attagcggtt
    12241 gttgggtttg gattatttgc agcagtttta ggtcctttag tttttatgtt cggtttgttt
    12301 atcagcgtga tggggaatgc aatgacagtt ttaggaccct tgttaataaa cgttaataaa
    12361 gctggtagta tattcgcgtt tttaagaact aaaatcgcat cacttgttaa actatttccg
    12421 attttaggtg tgtcgatatc cagtttaacg ttacccataa cattaattgt aggtgcatta
    12481 gttggtattg gcatagcttt ctatcaagct tataaacgtt cagaaacttt tagaaatatt
    12541 gtaaatcagg caatctctgg tgtagcaaac gcatttaaag cagctaaact agcgttacaa
    12601 ggtttctttg atttattcaa aggtgatagt aaaggcgcgg ttaccctaga gaagatattt
    12661 ccacccgaaa ctgtagcagg aatacaaaat gtagttaata cgattagaac aactttcttt
    12721 aaagtagttg atgcaatcgt tggtttcgcc aaagagatag gcgctcaatt agcctctttc
    12781 tggaaagaga acggctcaga aataacacaa gctttgcaaa atatagctgg tttcattaaa
    12841 gcaacctttg aatttatttt taactttatt attaaaccaa tcatgtttgc gatttggcaa
    12901 gtgatgcaat ttatttggcc ggcggttaaa gctttgattg tcagcacttg ggaaaatatc
    12961 aaaggtgtaa tacaaggggc tattaatatt attttgggta ttatcaaagt gttctctagt
    13021 cttttcacag gaaactggcg aggcgtttgg gacggcattg taatgatact gaaaggtact
    13081 gtgcagttaa tttggaattt aatacaactg tgctttgtag gtaaaattct aggtgtagtg
    13141 agatactttg gtggattact taaaggttta ataactatta tatgggttgc tataataggc
    13201 gttttcaaga aatcattatc ggcaatttgg aatgcaacaa aaagtatttt tggtttctta
    13261 ttcaatagtg ttaaatctat tttcactaat atgaaaaact ggttatctag tacgtggaat
    13321 aatatcaaaa gcaataccgt cggcaaggct cattcgttat ttacgggtgt aaggtctaaa
    13381 ttcacaagtt tatggaatgc gacgaaagat atatttacta aattaagaaa ttggatgtca
    13441 aacatctgga actctattaa agataacact gtaggtatag ctggtcgctt atgggataga
    13501 gtgcgtaaca tctttggaag catgcgtgac ggtttaaaat ctatcattgg taaaattaaa
    13561 gatcatatcg gtggtatggt agacgctgtt aaaagaggtc ttaataaatt aattgaaggt
    13621 ttaaactggg tcggtggtaa gttgggtatg gacaaaatac cgaagttaca cactggtact
    13681 gaacatacgc atactactac aagattagtt aagaacggta agattgcgcg ggatacgttc
    13741 gctacggttg gggataaagg acgtggaaat ggtccgaatg gtttcagaaa tgaaatgatt
    13801 gaattcccta atggcaaacg ggtacttacg cctaatacag atacgacagc gtacttacct
    13861 aaaggttcaa aagtatataa cggcgcacaa acttattcaa tgttaaatgg aacgcttcca
    13921 agatttagca taggtactat gtggaaagat attaaatccg gtgcatcatc ggcatttaac
    13981 tggacaaaag atcaaatagg taaaggtaca aagtggcttg gcgataaagt tggtgatgtc
    14041 atggacttta tcgataatcc aggcaaactt ttaaattatg tacttcaagc gtttggagtt
    14101 gatttcagtt ctctaactaa aggtatgggt attgctggcg atataacaaa agctgcatgg
    14161 tctaagatta agaaaagtgc aatcaagtgg cttgaggatg ctttcgcaga gtcgggtgat
    14221 ggcggtgtat tagatatgaa taaattacgt tacttatacg gtcacactgc tgcttataca
    14281 cgagaaaccg gacgcccatt ccatgaaggt ctggattttg attacattta cgaacctgtt
    14341 ccatcaacca ttaatggtag agcacaagtt atgccttttc ataatggtgg ttatggaaaa
    14401 tgggtgaaaa ttgtaaaggg cgccttagaa gttatttatg cacatttatc taaatataaa
    14461 gttaaaactg gtcaacaagt tagggtcggc cagactgttg gtatatcggg gaatacgggg
    14521 tttagtacag gacctcactt acattatgag atgcgttgga atggaagaca tagagacccg
    14581 ttaccgtggt taagaaagaa taatgggggc ggcaaaagta cacccggtgg taatggtgca
    14641 gctaatgcta gacgagctat taaggctgct caaaatattt taggaggaag gtataaggcg
    14701 agttggatta ctaacgagat gatgcgtgtt gcgagtcgtg aatccaatta tacagctaat
    14761 gcagtcaata attgggatag caacgcaaga gctggtatac cttcaagagg tatgttccaa
    14821 atgatagatc cttcatttag agcgtacgca aagtcgggtt acaataatcc tctcaaccca
    14881 actcatcaag ctatatcggc tatgagatat attgtgggta aatgggtacc aagaacaggc
    14941 tcatggagag ctgcgttcaa acgcgctggt gattacgcat atgctactgg tggcaaagtc
    15001 tataacggat tgtaccactt aggggaagaa ggatatccag agtggataat acctactgat
    15061 ccaagtagag cgaacgaagc acacaaatta ttagctttag ctgctaacga tattgataac
    15121 cgctctaaaa ataagcgacc aaacaactta ccaaatccaa gtataagtaa tagtgataca
    15181 aactatattc atacattgga gaataaactg gatgcggtta ttaattgttt ggttagtttg
    15241 gttgagtcta atcaagttat tgcagataag gattacgaac cagttattaa taagtatgtg
    15301 tttgaagatg aggtaaataa ttctatcgat aaacgagagc gtcacgaatc tacaagagtt
    15361 agatttagaa gaggaggcac gataatctaa tgcaagatac aattcaaata gacaataaaa
    15421 ccattgaatg gttagttgta caaagagggt ttgagatacc ctcttttaat tttgttactg
    15481 aaaaagaaag tgtgaaaggt agaacaggtt ctattgctaa agctcgttat ctaaatgata
    15541 tcgaatttga attacctcta ataattagaa atgaagtctt ggcgccaggt ggacaaaaaa
    15601 cgcatgacga tatattggaa gagttagttg aattttttga tattgacaat ttaaagccga
    15661 aaaaacttaa attcaaatct caaaactggt attggtttgc atactttgat ggaccattaa
    15721 aattaccgaa aaacccaaga ggttcagtga agttcactat caaagtagta ctaacagacc
    15781 cttacaaata ttcagtaaca ggaaataaaa atactgcgat ttcagaccaa gtttcagttg
    15841 taaatagtgg gactgctgac actcctttaa ttgttgaagc ccgagcaatt aaaccatcta
    15901 gttactttat gatcactaaa aatgatgaag attattttat ggttggtgat gatgaggtaa
    15961 ccaaagaagt taaggattac atgcctcctg tttatcatag tgagtttcgt gatttcaaag
    16021 gttggactaa gatgattact gaagatattc caagtaatga tttaggtggt aaggtcggcg
    16081 gtgactttgt gatatccaat cttggcgaag gatataaagc aactaatttt cctgatgcaa
    16141 aaggttgggt tggtgctggc acgaaacgag ggctccctaa agcgatgaca gattttcaaa
    16201 ttacctataa atgtattgtt gaacaaaaag gtaaaggtgc cggaagaaca gcacaacata
    16261 tttatgatag tgatggtaag ttacttgctt ctattggtta tgaaaataaa tatcatgata
    16321 gaaaaatagg acatattgtt gttacgttgt ataaccaaaa aggagacccc aaaaagatat
    16381 acgactatca gaataaaccg ataatgtata acttggacag aatcgttgtt tatatgcggc
    16441 tcagaagagt aggtaataaa ttttctatta aaacttggaa atttgatcac attaaagacc
    16501 cagatagacg taaacctatt gatatggatg agaaagagtg gatagatggc ggtaagtttt
    16561 atcagcgtcc agcttctatc atagctatct atagtgcgaa gtataacggt tataagtgga
    16621 tggagatgaa tggattaggt tcattcaata cggagattct accgaaaccg aaaggcgcaa
    16681 gggatgtcat tatacaaaaa ggtgatttag tgaaaataga tatgcaagca aaaagtgttg
    16741 tcatcaatga ggaaccaatg ttgagcgaga aatcgtttgg aagtacttat ttcaatgttg
    16801 attctgggta cagtgaatta atcatacaac ctgaaaacgt ctttgatacg acggttaaat
    16861 ggcaagatag atatttatag aaaggagatg agagtgtgat acatgtttta gattttaacg
    16921 acaagattat agatttcctt tctactgatg acccttcctt agttagagcg attcataaac
    16981 gtaatgttaa tgacaattca gaaatgcttg aactgctcat atcatcagaa agagctgaaa
    17041 agttccgtga acgacatcgt gttattataa gggattcaaa caaacaatgg cgtgaattta
    17101 ttattaactg ggttcaagat acgatggacg gctacacaga gatagaatgt atagcgtctt
    17161 atcttgctga tataacaaca gctaaaccgt atgcaccagg caaatttgag aaaaagataa
    17221 cttcagaagc attgaaagat gtgttgagtg atacaggttg ggaagtttct gaacaaaccg
    17281 aatacgatgg cttacgtact acgtcatgga cttcttatca aactagatat gaagttttaa
    17341 agcaattatg tacaacctat aaaatggtat tggattttta tatagagctt agttctaata
    17401 ccgtcaaagg tagatatgtg gtactcaaaa agaaaaacag cttattcaaa ggtaaagaaa
    17461 ttgagtatgg taaagatttg gttgggttaa ctaggaagat tgatatgtca gaaatcaaaa
    17521 cagcattaat tgctgtggga cccgaaaacg acaaaggaaa gcgtttagag ttagttgtga
    17581 cagatgacga agcacaaagt caattcaact tacctacccg ttatatttgg ggaatatacg
    17641 aacctcaatc agatgatcaa aatatgaatg aaacacggtt gcgttcttta gccaaaacag
    17701 agttaaataa acgtaagtcg gcagttatgt catatgagat tacttctact gatttggaag
    17761 ttacgtatcc gcacgagatt atatcaattg gtgatacagt cagagtaaaa catagagatt
    17821 ttaacccgcc attgtatgta gaggcagaag ttattgccga agaatataac ataatttcag
    17881 aaaatagcac atatacattc ggtcaaccta aagagttcaa agaatcagaa ttacgagaag
    17941 agtttaacaa gcgattgaac ataatacatc aaaagttaaa cgataatatt agcaatatca
    18001 acactatagt aaaagatgtt gtagatagtg aattagaata ctttgaacgc aaaatacaca
    18061 aaagtgatac accgccagaa aatccagtca atgatatgct ttggtatgat acaagtaacc
    18121 ctgatgttgc tgtcttgcgt agatattgga atggtcgatg gattgaagaa acaccaaatg
    18181 atgttgaaaa attaggtggt ataacaagag agaaagcgct attcagtgaa ttaaacaata
    18241 tatttattaa tttatctata caacacgcta gtcttttgtc agaagctaca gaattactga
    18301 atagcgagta cttagtagat aatgatttga aagcggactt acaagcaagt ttagacgctg
    18361 tgattgatgt ttataatcaa attaaaaata atttagaatc tatgacaccc gaaactgcaa
    18421 cgattggtcg gttggtagat acaaaaactt tatttcttga gtatagaaag aaattacaag
    18481 atgtttatac agatgtagaa gatgtcaaaa tcgccatttc agatagattt aaattattac
    18541 agtcacaata cactgatgaa aaatataaag aagcgttgga aataatagca acaaaatttg
    18601 gtttaacggt gaatgaagat ttgcagttag tcggagaacc taatgttgtt aaatcagcta
    18661 ttgaagcagc tagagaatcc acaaaagaac aattacgtga ctatgtaaaa acatcggact
    18721 ataaaacaga caaagacggt attgttgaac gtttagatac tgctgaagct gagagaacga
    18781 ctttaaaagg tgaaatcaaa gataaagtta cgttaaacga atatcgaaac ggattggaag
    18841 aacaaaaaca atatactgat gaccagttaa gtgatttgtc caataatcct gagattaaag
    18901 caagtattga acaagcaaat caagaagcgc aagaagcttt aaaatcatac attgatgctc
    18961 aagatgatct taaagagaag gaatcgcaag cgtatgctga tggtaaaatt tcggaagaag
    19021 agcaacgcgc tatacaagat gctcaagcta aacttgaaga ggcaaaacaa aacgcagaac
    19081 taaaggctag aaacgctgaa aagaaagcta atgcttatac agacaacaag gtcaaagaaa
    19141 gcacagatgc acagaggaaa acattgactc gctatggttc tcaaattata caaaatggta
    19201 aggaaatcaa attaagaact acaaaagaag agtttaatgc ttctaaaaga acactatcaa
    19261 gagtgttagc agacatcact gtaaatgcta tgaaaggcat ctatttaagg tatgacgaaa
    19321 atggggcgat tacttcacat actattgata aagatggcgt gaaaattagt ggcgataaag
    19381 ttgatataac agcgaataga gaatttaatg tagtcgcaaa taatattaat aacaaagttg
    19441 gtaaaaatga cattgttaat agcctaaact tatcaaatga aggtcttgac atcaatgtga
    19501 atagaattgg tattaaaggc ggaaatgcta accgttatgt acaagttcaa aatgatttta
    19561 ttgaacttgg cggaatcgta caacgaactt ggaaaggcaa acgatcaacc gatgatatat
    19621 tcacacgtct taaagatgga catctaaggt ttagaaataa taccgcaggc ggttcacttt
    19681 atatgtcaca ttttggtatt tcaacatata ttgatggaga aggcgaagac ggaggttcat
    19741 ccggtactat tcaatggtgg gataaaactt acagtgatag cggtatgaat ggcataacaa
    19801 tcaattctta tggcggtgta gtcgctttaa catctgacta caaccgaatt attatcgatt
    19861 catatgcttc agctaatatt gaaagtagag aagcaccgat atatttatct ccgaacacca
    19921 aaaataaacc tggtttaaac cgattcgcat tcacattatc aaacgctgat agtgcatacg
    19981 aaactgacgg ttatatcatg tttggttcag atgaaaacta taagtacggt gctggattaa
    20041 gattttctaa acgtagcaat aaaggattgg ttcaagtcgt taatggtgac tatgctacag
    20101 gcggagacac tacaattgag tcaggtatgg gcaaattcaa catagttaaa cgaagagatg
    20161 gaaatagtta cgttagcatt caaagttatg atttattggc ggtaggttct gataatgctg
    20221 gcgatagagt cgcttctaat tctatttata agcgtactta ttcagcacct gctaacttac
    20281 acattacttc tgctggaaca attgggcgtg ctacttctgc caaaaagtat aaaatttcaa
    20341 tcgaaaatca atacatcaat gaagacgatc agttcagtca ttcaaaagag attttaaagc
    20401 ttccaattcg tacatggttt gacaaatatg aatcggaaat aatggctaaa gaattggaaa
    20461 gtggtaaaaa gttatctgat gatactttta aacttagtcg acatactggc ttaatagcgg
    20521 aagaggttga agaattagga tttaatgaat ttgttattta tgatgacaac ggagaaatcg
    20581 aaggtatcgc atacgataga ctttgggttc atttaatacc tattattaaa aaccagcaat
    20641 caaaaatcga aaaactggag gaattaataa atgaatgata gcaatcaagg tttacaagcc
    20701 aatccacaat atacaattca ctatttatcg caagaaatca caagactaac acaagaaaat
    20761 gcaatgttaa aagcatatat acaagaacaa aatgaaaaaa gcaaaagtgc tgaggaagag
    20821 taatccttgg cactattttt atacaaaaat ttaaggaggt catttaatat ggcaaatgaa
    20881 attatcaaaa aaacagagag atttatttta gtacaaattg acaaagaggg aacagagcgc
    20941 gttttgtatc aagattttgt aggcagtttt acaacgtccg attcagcaag ttatgcacaa
    21001 gattttaaat ctgaggaaaa cgctaaaaag attgctgaaa ctttaaatct tttatatcaa
    21061 ttaacaggca atcaaaacgg tgtgaaagtt gtgaaagaag ttgtggatag aactgacttg
    21121 tcatctgata aatcagttga tagcgaaata atgtaactat actaagttat gagcattacg
    21181 ctcatagctt tcttagaaag taggtgtagt tttggatgat attcagaaaa taaaaaaaga
    21241 gctttctgaa ttagttgaac gtgttgatga tgttgaaata ctagcaaacg aaacagctga
    21301 tcatgtgctt gaacttagag aggaacataa gcaacatcat aatgaactaa gagaatctca
    21361 taaagaactt aaagataagc aagataaagt tgtagatgag aatttagagc aaacaaagat
    21421 attaaacaga attgaagaaa gatatcaaac gcaagtagat gttgcgcaaa aaaatgaaga
    21481 aaagacactc gcccaaaata aatggctcgt aggtgccata tgggcgcttg taacaattgt
    21541 tatgattgca gtcattactg catcaattac tgcgttatta ccttaaggga ggtggacata
    21601 atgagttggg caagatggtt atcatgttat ttgtatggtc gtaaatgtaa ataatgtttt
    21661 tggtcagtgc atcggcactg gctttttatt tattgttgta attatggtaa tatgcagaag
    21721 tgagcaagtt ggatagatgg tggctatctg agtataagga ggtggtgcct atgttggcat
    21781 tactgaaatc tttagaaagg agatgcctaa tgattacaat tagtaccatg ttgcagtttg
    21841 gtttattcct tattgcattg ataggtctag taatcaagct tattgaatta agcaataaaa
    21901 aataaccatc gctaactttg gctggtttcg atggttaaat ggttattaat ttaatcttta
    21961 atctaaaata gccaccgtct ttttaacggg ctcattaggg taacatgttt gcgcatgttg
    22021 ccctttttct atatataaat taacacacca taatataaat atcaaataga cggcttatta
    22081 gtcgtctttt tattttgggt aaaaggagat aagaatatga ttaattggaa aattagaatg
    22141 aaacaaaaat cattttgggt agcgatattg tcagctatct ttttatttgc tcaaaacatc
    22201 gcaaaagcta ttgggtatga tatccaagtt tatacagagc aattaacaga cggtttaaac
    22261 gctatattag gatttttagt attaactggt gtgattcaag acccgactac taaaggtata
    22321 ggtgatagcc accaagcttt agaatatgaa gaaccaagaa gaaaatacta ggaggtaaaa
    22381 taatgaaaac atacagtgaa gcaagagcaa ggttacgttg gtatcaaggt agatatattg
    22441 attttgacgg ttggtatggt taccaatgtg cagatttagc agttgattac atttattggt
    22501 tgttagaaat tagaatgtgg ggaaatgcaa aagatgcaat caataacgat tttaaaaaca
    22561 tggcaacagt atatgaaaac acaccatcgt ttgttccaca aataggtgat gtggctgtat
    22621 ttaccaaagg aatatataaa caatacggtc atattggttt agtgtttaat ggtggtaata
    22681 caaatcaatt tttaattttg gaacagaact atgacggtaa cgcaaatacg cctgcaaagt
    22741 tacgttggga taattattac ggctgtactc actttattag acctaagtat aaaagtgagg
    22801 gcttaatgaa taagatcaca aataaagtta aaccacctgc tcaaaaagca gtcggtaaat
    22861 ctgcaagtaa aataacagtt ggaagtaaag cgccttataa ccttaaatgg tcaaaaggtg
    22921 cttattttaa tgcgaaaatc gacggcttag gtgctacttc agccactaga tacggtgata
    22981 atcgtactaa ctatagattc gatgttggac aggctgtata cgcgcctgga acattaatat
    23041 atgtgtttga aattatagat ggttggtgtc gcatttattg gaacaatcat aatgagtgga
    23101 tatggcatga gagattgatt gtgaaagaag tgttttaatt cttaggttaa aat
//
LOCUS       DAMZLE010000029        22744 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMZLE010000029 DAMZLE010000000
VERSION     DAMZLE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.029"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(243..551)
                     /locus_tag="POB92_001014"
     CDS             complement(243..551)
                     /locus_tag="POB92_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDI6578524.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(566..994)
                     /gene="ruvX"
                     /locus_tag="POB92_001015"
     CDS             complement(566..994)
                     /gene="ruvX"
                     /locus_tag="POB92_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI6578525.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(998..1258)
                     /locus_tag="POB92_001016"
     CDS             complement(998..1258)
                     /locus_tag="POB92_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDI6578526.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(1321..3951)
                     /gene="alaS"
                     /locus_tag="POB92_001017"
     CDS             complement(1321..3951)
                     /gene="alaS"
                     /locus_tag="POB92_001017"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI6578527.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIENQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(4294..6771)
                     /locus_tag="POB92_001018"
     CDS             complement(4294..6771)
                     /locus_tag="POB92_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDI6578528.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(6773..7441)
                     /locus_tag="POB92_001019"
     CDS             complement(6773..7441)
                     /locus_tag="POB92_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6578529.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(8022..9140)
                     /gene="mnmA"
                     /locus_tag="POB92_001020"
     CDS             complement(8022..9140)
                     /gene="mnmA"
                     /locus_tag="POB92_001020"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI6578530.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKIEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(9141..10283)
                     /locus_tag="POB92_001021"
     CDS             complement(9141..10283)
                     /locus_tag="POB92_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6578531.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMMIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDGNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            10595..11608
                     /locus_tag="POB92_001022"
     CDS             10595..11608
                     /locus_tag="POB92_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6578532.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            complement(11845..11991)
                     /locus_tag="POB92_001023"
     CDS             complement(11845..11991)
                     /locus_tag="POB92_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578533.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(12031..12213)
                     /locus_tag="POB92_001024"
     CDS             complement(12031..12213)
                     /locus_tag="POB92_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6578534.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(12313..12735)
                     /locus_tag="POB92_001025"
     CDS             complement(12313..12735)
                     /locus_tag="POB92_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI6578535.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            12820..14094
                     /locus_tag="POB92_001026"
     CDS             12820..14094
                     /locus_tag="POB92_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDI6578536.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(14255..15028)
                     /locus_tag="POB92_001027"
     CDS             complement(14255..15028)
                     /locus_tag="POB92_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDI6578537.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(15028..15156)
                     /locus_tag="POB92_001028"
     CDS             complement(15028..15156)
                     /locus_tag="POB92_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578538.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            complement(15221..15411)
                     /gene="ssrS"
                     /locus_tag="POB92_001029"
     ncRNA           complement(15221..15411)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="POB92_001029"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15489..17255)
                     /gene="aspS"
                     /locus_tag="POB92_001030"
     CDS             complement(15489..17255)
                     /gene="aspS"
                     /locus_tag="POB92_001030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI6578539.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(17271..18533)
                     /locus_tag="POB92_001031"
     CDS             complement(17271..18533)
                     /locus_tag="POB92_001031"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI6578540.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            complement(18994..19869)
                     /locus_tag="POB92_001032"
     CDS             complement(18994..19869)
                     /locus_tag="POB92_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI6578541.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            complement(19866..20318)
                     /locus_tag="POB92_001033"
     CDS             complement(19866..20318)
                     /locus_tag="POB92_001033"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDI6578542.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            complement(20330..22519)
                     /locus_tag="POB92_001034"
     CDS             complement(20330..22519)
                     /locus_tag="POB92_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI6578543.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
BASE COUNT         6931 a         4429 c         3182 g         8202 t
ORIGIN      
        1 acaataaaat aaatcagcct tcacaattga ttataatttt gccaaccaat taaatgactg
       61 atttcgtgtt agacgcaaag ctattttatt tatagaagcg aatcattcat ataaaattta
      121 actttagata ttttaccata ttttcaataa aattataagc gttaattatt tatacattgc
      181 ttaacttaaa aaaatactct tgcctcccca tcttaaggtt agcaagagta aaatcttttt
      241 aattattctt ccatttcagt atttacaact tcttcaatca tgtcccattc ttcatcagtt
      301 tcgattggta ctaacttacc accgtcacct gactcatctg gttcattgat cattggtaca
      361 agctcaatca tatcgtcttc atctgattga gcaccttctt cagctaagat aacatactct
      421 tttttgaatt caggatgata aaattctaaa acttttcggt ataaaacttc atttccctct
      481 tcatcgaata aagttaataa ttcttcttcg ttattaattt ctagttgtga atcatgatta
      541 tgttcagtca tagtaaaatc tccttttaat gtagtgaatc taaatagcct tgtaaaataa
      601 ataccgctgc cattttatca atcacttgtt ttcttttttg tcttgaaaca tctgcttcta
      661 ataatgatcg ttcagcagcc attgtgctta atctttcatc ccacatcaca atctcaatag
      721 aaggataagc ttctaataat ttttctttat atgttaacga agcttcgcct cgaaatccta
      781 ttgaattatt catgttttta ggtagtccta ttacgactgt acccacatta tgttttttaa
      841 taatgtctac taattggtca atacctaatt cattattttc ttcattgatt cggagtgtgt
      901 ctaatccttg tgccgtccaa cccattatat cactaattgc aattcctacc gttctactac
      961 cgacatcgag tcctaaaatt ttatgttgta acataaatta tttattttgc tcttttaaat
     1021 agtaagaaac aagttcttcc ataataacat ctctatcaat atgacgaatt tgatttcttg
     1081 cttcattttg gcgtggaata tacgcagggt cacctgataa taaataacct acaatttggt
     1141 ttacggcatt atatcctcgt tcatctaatg ttcgataaac attatttaaa acatctctta
     1201 catcttgcgt tggaagttct tcatagtcga atttcattgt tttatcaaag ttttccattt
     1261 gcgacactcc tttaattaca aatataactc actatcatca tacaatatta tggctttaaa
     1321 ttatagattt ttaatgtaat ctttaataaa gcttaatgat tttgagatat tttcaggttg
     1381 tgtaccgcca ccttgagcca tatctggacg accgccacct ttaccaccaa cgattggtgc
     1441 catttgtttg ataagatcac cggctttaac gttatttgtt aaagatttag ggacagttgc
     1501 aaccatcgat actttatcat caacattact tgcaagaatg ataattgtat cttgtagttt
     1561 agatttaaaa tcgtccattg tcgagcgaat tgctttcgca tttggtacat ccacttcagt
     1621 aaccaatact ttatagccat tgatttcttc aacttgattt tcaatattac ccattttaag
     1681 tgatgtgatt tctttgtcac gttgctctaa ttgttttaat aatgcttttt cttcatcttg
     1741 taattgtgtt aacttatcga ctacttgatc atcagatttc actttcagct gtgatttcat
     1801 cgtattaaat ttctcttgaa tatcttctaa atataagaaa gctgctttac ctgttaatgc
     1861 ttcaatacga cgcacaccag ctcctgtacc tgactcactt actattttga ataagccaat
     1921 ttcagaagta ttgcggacat gaataccacc acataattca attgaaaatg gtgccatatt
     1981 tactacacgc acaacatcac catatttttc accgaataat gccattgcgc ccatttcttt
     2041 agctgaagca atatccattt cttgaatgtt aacgtcaata cctttccaaa tttcttcatt
     2101 tactaagcgt tcaacttgat caatttcatc attagtcatt ggaccaaaat gagagaaatc
     2161 aaaacgtaaa cgatctgctt ctactagtga accagcttgg ttaacatgat cacccagtac
     2221 tgatttcaac gctgcatgta ataaatgtgt tgcactatgg ttcttttgaa tgtcacgtcg
     2281 atcattttgg ttcacttcag cagacactgt agcgccaaca tttacttggc caaattgtac
     2341 tactccttta tgcaagtttt gaccatttgg tgctttggtt acttcactaa cagcaatttc
     2401 aaaattgtca ttataaacaa tacctgtatc cgcaacttgt ccaccactga ctgcataaaa
     2461 tggtgtttcc gttaacatga agtatactgt ttcacccgct tcaacttgtg aaacttcttc
     2521 accattatat atcaagtgtg ttagtgttgt ttgagctgtc gcagtatcat aaccaacaaa
     2581 agtacttgca gatgtaatat ttttcaatac ttcactttga acttgcattg attgagaatt
     2641 ttgacgtgct tgacgtgcac gatcacgttg ttgttgcatt tctgactcga atgttgtcat
     2701 atcaactttc aatcctgctt gcactgctat ttcttcagtt aattcaattg ggaacccgta
     2761 cgtatcatac aatttaaatg catctttccc attaatttca tttgttgtcg ctttagcttt
     2821 tttaattaat tcatttaaaa tcgctaaacc atcttctaat gtttcatgga atcgttcttc
     2881 ttcagacttt ataacacgct taatgaaatc tgctttttcc ttaacatttg gataatatgg
     2941 ttccataatg tctgcaacaa tatcaacaag tttgtacata aatggctcat tgattcctaa
     3001 cgtttgacta aaacgaacgg cacgacgtaa caatcgacgt aatacatatc ctctaccttc
     3061 attggcaggt aatgcaccat cagaaattgc aaatgcaatc gtacgaatgt ggtcagcaat
     3121 tactttaaat gccacatctt gttcgttgtt tactaaatat tgtttacctg atactttttc
     3181 gatttcattc attataggca taaataaatc tgtttcatag ttagtacgta cattttgaga
     3241 aactgaggcc atacgctcaa gccccatgcc agtatcaatg tttttattag gtaatggtgt
     3301 gtaactatga tctttattat gattgaattc actaaatact aagttccata cttcaagata
     3361 gcgttcattt tctccacctg gatacatttc ttctgccgga tcgtcttgtc catatgcttc
     3421 tccgcgatca tagaaaatct cagtgtttgg tcctgaaggc ccttcaccaa tatcccagaa
     3481 gttaccttca atgcgaataa tacgactttc ttcaagccca atatctttat gccaaatgtt
     3541 gtatgcttcc atatcttccg gatgaatcgt aacgtacaat ttatctggct ccatccccat
     3601 ccatttatca ctcgttaaaa attcccaagc aaattcaatc gcttcttgtt taaaataatc
     3661 accaattgag aagttaccta acatttcaaa gaatgtatgg tgacgcgctg tgaaaccaac
     3721 attttcaata tcatttgtac gaatagcttt ttgagagttt acaattcttg gctttttagg
     3781 tgtttcacgt ccatcaaaat atttctttaa tgttgctaca cctgaattaa tccataataa
     3841 tgtatcatca tcaattggca ctaatggtgc agaaggttca accatatgtc ctttttcaac
     3901 aaagaaatct agatattttt gtctaatttc actcgctttt aactttttca tcttttacac
     3961 atcctattta ctgtttttaa attaccattc cataaaaatt gatgacacag atagtcgatt
     4021 tgaaaaacta gtataaatca atatcatttt ttattattaa aaaataaaaa acgcccatcc
     4081 tcaaaaggga cgaacgttat cgcggtacca ccctagttat aaatgcaatt caacacattt
     4141 atcactttaa atcgactata cagttgtgca taaagtagcg ttcactaatg tttgttgtac
     4201 ttttcaccaa ccagtacatc tctgataaac aaatcattaa ctactcatct ttatacgaat
     4261 ttaattctat tttagttaca tttactcttg ttgtcaacgt tctataaagt catacggcgt
     4321 gatttctccc atattaatca ttgggtcaat tttaaacatt gtagcttccg ttaatacatt
     4381 tatatctgtt tttgttgaat cagacgtaac ttctccacta tcattcgatg acattggcgc
     4441 ttctacttga tcatctattg tcgtttgtga agctcctgta tcattagttg ctgtgttttc
     4501 cagcatttct tcatcttctg aattaaaata atttttcaac aatgtacata attgtgttaa
     4561 acgcgcttga ccatttgttt ttaatccaat atcaaatgct tctggatcac caagtagaac
     4621 taaactcgtt ttcgctctag ttaaaccagt atataatatc ggtctttgta acattctaaa
     4681 atactgttta acaataggca tgataacaat aggaaattct gaaccttgtg atttatggat
     4741 tgatgtacaa taagcatgtg ttaattccat catatcttgt ttcgtaaatg taatttcatt
     4801 accttcaaaa tccacaacaa gtacatcttt attaagggca ttttctttcg cccaaaaaat
     4861 accaacaata actcctatgt caccattgaa tatgttatca tttggcctat taacaagttg
     4921 taatactttg tcaccttttc taaagactac atcaccaaac tcaatttctc gtgtgtcttt
     4981 ctttttaggg tttaaaatat cttgtaaaac ttgatttaaa cgtttaatac cggcatttcc
     5041 tttatacatt ggtgcaagca cttgaatatc agccatagta taccctttat taacagcact
     5101 agtaactacc ttctctacaa ctgttggtat ttggtttgcc tgacagttaa taaaacttct
     5161 atcatgaaaa cgctgtgtaa tatcaatttt ctgacccaac ttcattcgat gtgctaattc
     5221 tataatgctt gaaccatctt gttgacgata tacttcagtc agattaactc gtggtatagc
     5281 tttcgattca attaaatctt taaatacttg accaggacct acagaaggca attggtcctc
     5341 atcacctaca aatatcaatt gtgcatctaa aggaactgca cttaaaaatt ggtggaacaa
     5401 ccaagtatct accatagaca tctcatcaat gattatgagt cgtgcgttta tttcattttc
     5461 taatatatcc tctggctttg tgtcttgatt ccaacctatt aaacgatgaa tcgtcatagc
     5521 ttctaatcca gttgactctt gtagtctctt ggacgctctt cctgttggcg ctgctaatac
     5581 aactggataa tcatcattga cataatcatc ataatctaat gataagccat gaatctcagc
     5641 atataattca acaatacctt taattactgt cgtttttcct gttcccggtc caccggttaa
     5701 tagcatcacc ttagaattga tagccgtttg caaagcttct ttttgtgaag ctgcatagtt
     5761 cacttgattc gcatcttcta tttcaccaat atgcatttgt aaatctgact gttcaatttc
     5821 tgtaagttta tttgtatgcg tctttattct gaataagttt tgaacacttt tgatttcaga
     5881 ataatacaaa cttggaattg caacttgttc attgtcaata attagtcgtt tttcctcatt
     5941 taagtattgc aacatttcgt ctaatttttc aggttcgatg acctcttcat cttgataatt
     6001 taatacatca accgttaaat ctataacaac attgataggc aaatatgtat gtccctgttt
     6061 aatacattct tcttctaacg tatagagcaa cgcagctttt aatcgttcat tatcgttata
     6121 agcgatacca atatttctag caagttgatc tgctttatta aaaccaatac ctttaatatc
     6181 ataaatcaat tgatatggat ttcgatctaa aatagtcagt gtatcgccga gataaaactg
     6241 ataaattgcc attgaaagtt taggaccaaa ccctaaatca tgtaaacgaa tcattatttt
     6301 ttcagattct tgatttgctg aaatttgttc tgcaatttgt ttctgtttct ttttagataa
     6361 tcccgaaact ttttctagca ctgaatggtc atctaatata tcatttatcg cattgtcacc
     6421 taatgtatta acaatatttt gagctgtctt tttacctaca cctttaaaca aatcactaga
     6481 taaataactt ataattgctt ctttcgtttg tggcatttct ttttcaaaag tctctgcttt
     6541 taattgttta ccataacgtg gatgatcaac aacttgccct ttaaatgtgt agacatcgcc
     6601 ttcaacaata ttcggaagaa accctacaac agttggcatt gtatcaaagt cttcatttgt
     6661 ttcaatagta tctactttaa gcactgtata aaaattatca ctgttttgaa acaatatcgc
     6721 ttcaacagta cctttgatca ttgaataatc aaatagtgta gggtctgaca tgttactcct
     6781 cctctttcat tttagtgaat gttttcagcg catgctgact taataagtgt ttagggtcga
     6841 tagtcacagc ttctttaaaa tgagttattg cttcatcaat atcttcattt ttcataaata
     6901 acgctaagcc caaattgtat cttgcatcaa catgattttt atcaatcgtt aatacatgtt
     6961 taagttgagt tatggcttca ttaaacattt ctaattgaca taatacaaga ccatattgaa
     7021 attgaacttc tgcatctttg tctttatcta gttccgcagc agtcattaaa tacggcaatg
     7081 ccagcttaaa tgattctaac tgattaaacg ccataccgat catataatta caatcaactt
     7141 gttcaatctc tgtttgtaat gcttgttgat ataatttaat agcttcttga taacgttgct
     7201 gattataata tacatttgct agattataaa atacgacgcc attcttcgga tctattgtta
     7261 aagctttttg gaaaaagcgc tctgcctttt caatctcatt cgcatcagca agtacgatac
     7321 cagcattaat ataattttca ataattgtag gattttcttc gatatttccg aacaatgctt
     7381 gtaacgcttc ttctattttt ccattttgta tgtattgata aattgtttgt tgatctatca
     7441 tttacgaacc tcatttctca tcaattataa catcttgata aattgtatgt ctcgaatcac
     7501 ttaacaacga ataaaatata atctaatatc atcttcattc atgaaaaagc gggaatggaa
     7561 tagaaatgat taagaaccat taacggttta ttatgtaatg gttcttccac attagccact
     7621 actattatgt acttaaaaat aggaatacat aattagactc atgcataggg agtgggacag
     7681 aaatgatatt ttaacaaaat taaattcgtt tatccccaac tcgcattgcc tgtagaattt
     7741 ctttacgaaa ttctctgtgt tggggcccca tccccaactt gcaaattatt gaaatctgac
     7801 ttttggtcag cttctgtgtt ggggccccgt ccccaacttc cattgcctgt tgaaattggg
     7861 gtcccaattt ctctgtgttg gggccccgcc aatataacat tgtagagcct aggacattgt
     7921 gatgtcccag actctatcat catgaattat tctcatcaaa aactgtcttt cgtcattttc
     7981 gacgttgaaa cttcaaataa gtaatttatt gttgccattg tttatacaac ataatttaat
     8041 tgaccttcat ttttgaacac atcgtcaatt gttgctccac caagacacac atcaccttga
     8101 taaaaaacaa ctgcttgtcc aggtgtgatt gctcttactg gctcagcaaa agtaacacgt
     8161 agtgcatggt cgttttcacg tttcacaaaa actttcgtat ctttttggcg atatctaaat
     8221 ttagctgtac attcaaaacc ttgatctaag tcattatctt ctggatttac aaatgaatag
     8281 tctgaagcaa ttaagtaatc actgtataat gcatcgtgat ggaatccttg ttctacatat
     8341 aaaacattat cttttagatt tttaccgaca acaaaccaag gatcgccatc tccacctata
     8401 cctaaaccat gtctttgtcc tattgtgtaa tacatcaacc cactatgttt acccattttc
     8461 ttaccatcaa gtgttatcat atcacccggt tgcgcaggta gatattgaga taaaaatgtt
     8521 ttaaagtttt tttcgccgat aaaacaaata cctgtagaat cttttttctt agcagtaact
     8581 agtccttgtt cttcagcaat tcgacgcact tcaatctttt cgatgtcgcc aattgggaac
     8641 atcacttttg aaagttgttg ttgggataat tgattcaaga agtatgtttg atctttatta
     8701 ttatctacac cacgtaacat ttcaacatga ccatcttcat gacgatgtat gcgtgcgtaa
     8761 tgccctgttg ctacataatc tgcacctaaa ttcatcgcat gatctaaaaa ggctttaaac
     8821 ttaatttctt tattacacat aacgtctggg tttggagtac gacctttttt atattcatct
     8881 aagaaatacg taaagacttt atcccaatat tctttttcaa aattaacagc gtaatatgga
     8941 atgccaattt gattacacac ttcaataaca tcgttgtaat cttcagttgc agtacatacg
     9001 ccattttcgt cagtgtcatc ccagtttttc ataaatatgc caatgacatc ataaccttgt
     9061 tcttttaaga cgtgggctgt tacagaacta tctacaccgc ctgacatacc aacgacaaca
     9121 cgtatatctt tatttgacaa ttatgactcc tccttaaatt taaaatatat tttatgaatt
     9181 tcagctacaa ttgcattaat ttcattttca gtagtcaatt cgttaaaact aaatcgaatc
     9241 gaatgatttg atcgctcttc atcttcgaac attgcatcta aaacatgcga cggttgtgta
     9301 gagcctgctg tacatgcaga tccagacgac acatagattt gtgccatatc caacaatgtt
     9361 aacatcgttt caacttcaac aaacggaaaa tatagattta caatatggcc tgtagcatcc
     9421 gtcattgaac catttaattc aaatggaatc gctctttctt gtaatttaac taaaaattgt
     9481 tcttttaaat tcattaaatg aatattgtta ccgtctcgat tcttttctgc taattgtaat
     9541 gctttagcca tcccgacaat ttgtgcaaga ttttcagtac ctgcacggcg tttcaattct
     9601 tgttcaccgc caagttgagg ataatctaat gtgacatggt ctttaactaa taatgcaccg
     9661 acaccttttg gtcctccaaa cttatgagca gtaatactca ttgcgtcgat ctcaaattcg
     9721 tcaaacttaa catcaagatg tccaattgct tgaaccgcat caacatggaa atatgcattt
     9781 gtctcagcaa taatatcttg aatatcataa atttgttgca ctgtgccaac ttcattattt
     9841 acaaacataa tagatactaa aatcgtctta tctgtaattg tttcttcaag ttggtctaaa
     9901 tcaatagcac ctgtatcatc aacatctaga tatgttacat caaaaccttc tcgctctaat
     9961 tgttcaaaaa catgtaacac agaatgatgt tcaatcttcg atgtgataat gtgattaccc
    10021 aattgttcat ttgcttttac tatgccttta attgccgtat tattcgattc tgttgcacca
    10081 ctcgtaaata taatttcatg tgtatctgca ccaagtaatt gtgcaatttg acgtcttgac
    10141 tcatctaaat atttacgcgc atctcttccc ttagcatgta ttgatgatgg attaccataa
    10201 tgcgaattgt aaatcatcat catcgcatct actacttcag gttttactgg tgtggtcgca
    10261 gcataatctg cataaatttc catgtttgga cactcctcac aattttatca atgttccaat
    10321 aatagcacct tacatactat ttttctactt ttctgtttaa ctttatttat aatgttttta
    10381 attatatttt accattttct acacatgctt ttcgataggc ttttttaagt ttatcgcttt
    10441 attcttgtct tttttataaa ttttagtatt tgcagatatt ttttcattcg taaaatgtaa
    10501 cgtactatta ttttggttat gagcaattta atatttatct ggttattcga ttggtatact
    10561 tcttatatca tacaaaagga aggacgatac aaaaatggcg attaaatatt cagcattaaa
    10621 ccttgtccct attcgagaag gtgaagatga acgaacagca attaatgata tggttaagct
    10681 cgcacaacat ttagacgaat tatcatatga aagatattgg attgctgaac accataacgc
    10741 tcccaaccta gtaagttcag cgactgcttt attgattcaa catacgttag aacatacgaa
    10801 acacatacgt gtaggttctg gaggaatcat gttacctaac catgctccat taatcgttgc
    10861 ggaacaattt ggcacgatgg caacattatt tccaaatcgt gtcgatttag gattaggacg
    10921 tgcacctgga acagatatga tgaccgcaag tgcattaaga cgagatcaac atgatggtgt
    10981 ttataaattt ccggaagaag tttcattatt acaacaatat ttcggtcccg ctcaccaaca
    11041 agcatatgtt cgtgcttatc cagcagtagg taaaaatgtg cctttatata ttcttggttc
    11101 ttcaacagat tctgcacatt tagctgctcg taaagggctt ccatatgtgt tcgctggaca
    11161 ttttgcacct caacaaatga aagaagctat cgaaatttac aaaacgttat ttgaaccttc
    11221 tgatgtatta gacgaacctt atgttattgt atgtttaaat acaatcgttg ctgaaaatga
    11281 tgacgaagca caatatttag cttcatctat ggcacaagta atggttagta tcactcgtgg
    11341 cagaatgcag cccgttcaac cgccaacaca tgaactacaa aatatattaa cgccgagaga
    11401 atatgcgatg gctatggaaa gacagaaaat atcattaata ggttcagaaa atactgttca
    11461 acaaaaaatt caagatttta tggaaactta tggtgaagtc aacgaaatta tggcaataag
    11521 ttatatttat gataaagata tgcaattaga ctcttatcgt cggttcaaga atgttataaa
    11581 tcagataaat gaaaaaaaca ctttataatg tgataaataa actaagtgaa agtatgtatc
    11641 cataatatta ataaaaataa acagtaacag cattttgaat aaaagatgtc tttattgttc
    11701 aatcatttat tttagtaatg attcaaattc acttaaaatt ctaaagcaaa tatgaaagcg
    11761 ccccttcaac tttacactgt gttagtgttt atttgatggg gcgctttcaa aatattgaaa
    11821 agcatatcca aaatttaaag aaatttattt ctctttatct tcattttctt ttttctcttc
    11881 gttattcgat cctgtatatt catttatctt atcttttaca tttttaactt gttcattatc
    11941 gctattttta aatttttcta ctgcgtcttt agctttatcc ataaaactca tattaatcgc
    12001 tcctcttata tttaattagt ttaattgaac ttatttttta agtttatcaa ttgcatcagt
    12061 tattttgttt ttagcatttt caacaacttc ttttgcttta ccagtcgctt tatcttgctg
    12121 accttctttt tctaattctt tgttatcagt aacgttacct actgtttctt taacatttcc
    12181 tttaaattga tcgaacttac tttcgtctgc catagtgaaa cctccttgga tgtatatatt
    12241 tatataccac taaggaggtt cgctaaacat tataatatga agtttttatg ttatagtata
    12301 gtatttatac gattaaatat aaaacatgta tccgtctaaa tcttcgcttg tatctacata
    12361 ttccgctaaa tatttcaatg ttgtattatc taaaacatct ctcactgcat ctctcatgcg
    12421 aatccatagt tgtttttgcg caggtggttc tgattcaata ctttcaacaa atgtaattgg
    12481 accttccaac agtcttataa tatcccctgc tgagatttct tccgctggca ctcttaattg
    12541 gtatccacct ttagcaccgc gtacacttcg aattaacccc gcatttctta aaggacctac
    12601 aagctgttct aaatataaat cactcaaatt attttcttca gcaattgact ttaatgatat
    12661 acatccttgc ccctcttttt tagcaagaga aatcatcaat gtaagtccat atctcccttt
    12721 agtagaaatt ttcattgtat aacctcactt aattcgaata ttgatattcc cattttagca
    12781 ttttttgagt taagatagta taagaaaggt gtgacaaatg tgagtacaga accattagca
    12841 tcgagaatgc gcccaaaaaa tatagatgaa atcatttccc aacaacattt agttggacca
    12901 agaggcatta tcagaagaat ggttgataca aaaaaattaa cttcaatgat tttttatggt
    12961 ccacctggta taggcaaaac aagtattgcc aaagcaattt cgggcagtac gcaatataaa
    13021 ttcagacaat tgaatgctgt aactaacact aaaaaagata tgcaacttgt tgttgaagaa
    13081 gctaaaatgt ctggtcaagt tatcttgtta ttagatgaaa tacatcgact agataaagct
    13141 aaacaagact ttttattacc tcatttagaa aatggcaaaa tcgtcttgat cggtgctaca
    13201 acttcaaatc cttatcatgc tatcaatcca gcgattcgtt caagagcgca aatttttgag
    13261 ttatatcctt taaatgatga agatgtgcgc caagcgttaa ctcgtgcaat agaagatgaa
    13321 gagaatggtt tgaaaacata tcaacccaaa attgatgaag atgccatgac ctacttttct
    13381 acacaaagtc aaggtgatgt tcgtagtgcg ttaaatgcat tggaattagc tgtattaagc
    13441 gcagataatg acaaagacgg ttatcgacat gttacattgc aagatgctaa agactgttta
    13501 caaaaaggtg catttgtaag tgataaggat ggggacatgc attacgatgt tatgagcgct
    13561 ttccaaaaat ctatccgtgg tagcgacgtc aatgccgctt tacattattt agcacgatta
    13621 attgaagctg gagatttacc tacaatagtt cgacgattac ttgtaattag ctatgaggat
    13681 ataggcttag cctcacctaa tgctggtcag agaacacttg ctgctattga atcagcagaa
    13741 cgtctaggtt taccagaagc tagaattcca ctaagccaag cagtaatcga actatgctta
    13801 tcacctaagt caaattcagc aatgagtgcc attgatagtg cattgtccga tattagaaac
    13861 ggtcatgtgg gccaaattcc aaaccattta aaagatggac attatcaagg tgctaaagat
    13921 ctaggccgat ctattggtta caaatatcca caccaatatg ttaatggcta tgtttcacag
    13981 caatatttac ctgataaact taaaaacaaa atttattatg aaccaaaaac gacatctaaa
    14041 agtgaacaac aactcaaaga aatatataac aacttactta aacaaaggcc gtaaaaataa
    14101 gattaaatcg attacctttg atattttagc tataatcaaa tcaaaactga ctagtatacg
    14161 aggcatattc tactatgctt gcgaactagt cagttttagt tttatggtgt ggttacactt
    14221 gccttacgtt gaaaattatg actttcaaaa tgaattattg ccctttgtct ttaatgcgac
    14281 gaactggaat atcttttaat acgtcattca ccacataact agcacaaatt aatccaacaa
    14341 cacttggcac aaaggcattt gaagaaggtg gcatttgtcc ttttcgattg atagcatttt
    14401 tatctccaac tatatctttt acatcttctc ttatgacaat tggactttca tctgaaaata
    14461 caactggaat ccctttacga attcctagtt ttttcaattt ttgacgaata attttggcca
    14521 ttggatcggt atgtgtttta gagatatctg aaattgtaaa acgtgttgga tctgttttat
    14581 ttgcagcacc catactggaa atcactttaa tccctcggtc aagacactct ttcattaagt
    14641 gtactttgta cattattgta tcacttgcat ctacaaaata atctatatcg tagttatcga
    14701 aaatttcttc atatgtctct tctgtataaa acatatgtaa gggcgtgact ttacaatctg
    14761 gattaattaa tttaatacgt tcttccatca aagaaacttt actttgtcct accgttgtag
    14821 ttaaagcgtg taattgtctg tttacatttg taatatcaac atcatcttta tctattaata
    14881 taatatgacc aatattcgtt cttgctaatg cttcagcagc aaatgaacca acacctccaa
    14941 cgccaagtat gacaacagtt tgttgcttca ataaatctaa accttgttgt ccaatcgcta
    15001 gttcatttct tgaaaattga tgtttcatta ttttacctct ttcactgatt tatacataag
    15061 tacatagtaa cttaaaattt tatatttagc attatcgctt tgattatttt cccaaaattc
    15121 aacgaggaaa catttattaa acgctataaa acccaactaa ttctttatta aaaacttaaa
    15181 gaaacgcata aaaatacgca agacaaagtc ttgcgtatcg atagagtccg tattgccgta
    15241 gttataatag cttgatcatt cggcctgtta tatacaggtg ggtgccctgt ttcttgtttt
    15301 gtacgtcctt catataaggc gtgtacgctg caagaaaacc cattgggctc ccttgatcaa
    15361 agagtgttag gcccaaatta aaaagcaaac ttacgaacaa ctcagatgac tatcttatga
    15421 tgttatatta ccacataatt aaaattaatg aaattataac aaaccaaagt ttattgattt
    15481 tttaaaattt agtgacgaat tcgcaaagaa agttcttcta attgtttatc agaaacttca
    15541 ccaggcgcat tcgttaataa acatgtagca gatgctgttt tagggaatgc gattgtatct
    15601 ctcaagtttg ttctattagt caataacatg actaatcggt ctaatcctaa tgcaataccg
    15661 ccatgtggtg gtgcaccata tttaaatgca tctagtaaga agccgaactg ttcttgtgct
    15721 tgttctttag taaatccaag aacttcgaac attttttctt gtaactcacc atcatgaatt
    15781 ctgattgaac cgccacctaa ttcataacca tttaatacta tgtcataagc atttgcctca
    15841 gcttcttctg gcgcagtgcc aagcttagca atatcagctt cttttggaga tgtaaatgga
    15901 tgatgtgctg caacgtaacg tttcgcatct tcatcatatt ccaataatgg ccaatctgtc
    15961 acccataaga agtttaattt tgtttcatcg attaaaccta attctttagc taatttgaca
    16021 cgtaatgcac ctaaactttg tgcaacgaca tttggtttgt ctgcaacaaa cattactaag
    16081 tcaccagctt cagcaccagt taatgtaagt aatgtttcaa cattttctgt ttcaaagaaa
    16141 cgtccaattg gacctgtcaa accatcttcc acaactttaa cccacgctaa tcctttagca
    16201 ccatagatgt ttacaaattc tgttaaagca tccatatctt tacgagtata ttgttcagct
    16261 gcacctttag cgacaattgc tttaatttca ccatcatttt caacagtatc tttaaatact
    16321 ttaaagtcca tatcacgtcc taattgagaa acgtcaatta attccatttc aaaacgcgta
    16381 tctggtttat cagaaccata gcgacgcatc gcttctttat atgtcatgcg tgggaaagcg
    16441 ccattaattt caacgccttt aacttcttta acaacttttt taagcatttc ttcacccatt
    16501 tgcatcacat cttcttggtc tacaaaactc atttcaatat cgacttgtgt aaattcaggt
    16561 tgacgatctg cacgtaaatc ttcgtcacgg aagcatttta cgatttggta gtatttgtca
    16621 aatccactaa tcatcaataa ttgcttaaat aattgtggtg attgtggtaa tgcataaaat
    16681 tcaccatcat gaacacgaga tggtactaaa tagtcacgtg caccctcagg tgttgacttc
    16741 gttagtactg gtgtttcgat gtcaaagaac ccttcatcat ccaaatattg acgaatagaa
    16801 cgtgtaattt gatgtctcat tttaaatgtt tgcgctaact cttgacgacg taaatctaaa
    16861 taacggtatt ttaatcgaat attttcatca acgttaacat tttcttcatt tatagaaaat
    16921 ggtggtgtct cagatttatt aatcacttta atatttgtaa cttgtacttc aacttggcca
    16981 gttttaattt taggattaac tgtttcaggg tcacgcttcg taactgtacc ttgaacttct
    17041 acaacatatt cagaacgtac tgtttcagca attttcaatg cctcttctga aaatgcagga
    17101 ttaaacacga cttgtacaat tccttctcta tctcttaaat caacgaaaat caatccacct
    17161 aggtcacgac gattgttaac ccatcctttt aatgtaattt cttgtcctaa aaatgcttca
    17221 gtaactaatc cacaataagt tgttctctta ctcatatttt aacgccctct ctacttctta
    17281 aaatattcga ctaatgcgtc taattcaatt gtttcagatt caccagttgt catattttta
    17341 acatcgattt tattattttc taattcttga tcaccaataa cgattgtaaa cttggcacct
    17401 aaacggtctg cttgtttcat ttgtccttta attttacgct gtaagtagtc tttatctgct
    17461 ttaataccat tatgtctcaa atgatttaat aacttcacag catatcgatc tgcttgatca
    17521 cccattgtaa caatgaataa atctaagttt tcttcaatat ctaattcgat accttcttct
    17581 tcaagtgcaa gcaataatcg ttctatactt agcgcaaaac caatacctgt ttcacttgga
    17641 ccatctagca attctaataa accattataa cggccaccac cacaaagcgt tgtaatggca
    17701 ccatcatagt tagggttatc catcattaat tcaaatgctg tatgtgtata ataatccaat
    17761 ccacgaacta agttaggatc ttcaatatat ggaataccta aatcatctaa ataagctttt
    17821 acttgttcat aatatgcctt agattcctca tttaagaaat cagtgattct aggtgcagtc
    17881 ttaatcgctt ctttatcacg gtcaacttta caatccaaaa ttcgcatcgg atttgtatgc
    17941 aaacgtgatt gacaatctga acaaaattca tgaattactg gttcaaagtg tttcactaac
    18001 gcttcgttat attcttttcg agacgccata tcccctacac tattaataac aagctttaaa
    18061 tgttttaatc caaatgattg ataaatatgc ataaccatag ctaatacttc tgcatctacg
    18121 ctaggatttt cagcaccaat agcttctaca ccaaattgat taaattgacg atagcgccct
    18181 ttttgcttac gttcatatct aaacatcggt ccattgtaat aaagtttaat tggttggttt
    18241 ggattacctt gcattttatg ttcaatatat gaacgcacaa ctgcagctgt tccctcaggt
    18301 cttaatgtaa tacttctatc gcctttatct ttaaatgtat acatttcttt ttgtacgaca
    18361 tcggttgaat caccaacacc tcttgcaaaa agatctgtac tttcaaaaat tggtgttctt
    18421 atttctttat aattataaaa tgtcattaat tcatctaatt gattttcaat gtaacgccat
    18481 ttctttgaat cttcaggtaa aatatcctgc gtccctctag gtattttaat cattttgaca
    18541 tctccctcgg caaactattt tatttaatta cctttttaaa actaaaacta tatacgatca
    18601 cttaactttg catgataaat cataatttta aaagcaaaag aaaaagccct tgcacagcct
    18661 gaaactgcac aagggacgaa tatccgtgtt gccaccctca ttggtttaaa aaacatgcct
    18721 aattgtaatc atcattactt tttataagcg tgcattaaat aaacccactc aaaacgttta
    18781 acgtacgttt aacggcttta cttttcataa agcacctctt cttgtgttca atgtatataa
    18841 actactcagc ataatctttc agtctatgga ttatattcct acacaatctt agttagattg
    18901 ctacctattt catttaccat ttcaagaata ctaacggttt tagttataaa attcaatatc
    18961 aatgataact actttcacat atttttgcaa gccctacgca gaaaaataaa ttttaaggcc
    19021 atcaacaatt gcttgttcta aaatttgtct atgtaattga tctttaatca tcgtttcatc
    19081 agttgggtta ctaatataac ctaattctaa taaaacagca ggaacttttg tttgtcttaa
    19141 cacttgataa ttttcttgtc ttgaaccgcg attagaaagt agacctttct tctgaatcgt
    19201 agcgtctaac gtatctgcta aagctctttg attatcatga taccaataaa ctgtcattcc
    19261 atttgcatta gatgattcta atgcatcatt atgtatactc aaataggcat cgcctttgat
    19321 atcacgattt tctagtgaaa catatgtatc gtctgttctt gtcatcttaa cagttgcgcc
    19381 ttctttttct aaagtacgct gcaattcttt tgctgttttc aacgtatagt ctttttctaa
    19441 acttttatat ttagtattgc ttgaagcacc ctggtcacta cctccatgac caggatcaag
    19501 cactattgtt ttaccttgca aaggattttt ctccttcgta ttatccgcga caatatctaa
    19561 atttgtgtgc catccagcta tccaaccttt ttcattactg gatgtatctt caacttcaat
    19621 ccatttacct actttaccaa tctttttaaa atggtcacct ttctcaactt tatatatgac
    19681 tggatacgca gcgtttggac ctgtacgtaa ttcagcattt tcagttatcg tgatgttccc
    19741 actatcttca ctattgctat tcagcaataa aaataaaaag atgataaata agacaaaggc
    19801 aatcactact attagagtac gtttattttt aagacccttt ttagataacc atgcctctat
    19861 ttttttcatt gaattttgcc gtcctgactt tcataaataa tagtgactgg accatcatta
    19921 tttatgctaa cattcatgtg tgttccaaat tcacctgttt tcacagtaag accatacgct
    19981 cgtagcgcat cattaaaata ctcataaatt tttaccgctt gatcaggatt tttagaattt
    20041 gagaaacctg gacggttacc tttttttaca tctgcataga gagtaaattg tgaaactgat
    20101 agtatttcac cattcatttg ttggatatta aagtttaatt tattattgtc atcttcaaat
    20161 aatcttgcat tagcaatttt ctttgcaatt acatctgcat cttgctctgt agagttctga
    20221 ccgataccga ctaataaaca atatcctttt ttgatttgat tatttaatgt atcattcgtc
    20281 accgatgctt ctttaactct ttgtacaact actttcatat tttgcacctc tagttccaaa
    20341 ctcttgttac tgtataaaca tcaccaagtt gtttgatctt ttctaccaca cgataaacat
    20401 cattcacgtt tttcaccatg acactaatat ttattattgc atttttatca atatctgaac
    20461 gtcctgaaac tttaattaaa ttgccggctg tcgagctaac agcttgtagt acttcattca
    20521 acaagccatt tcggtcatac gcagttacct ctaaatcaac ctgatatttt tgagttgcgt
    20581 cttttgattt tacccattca acattaatta gtcgttcagt ttcgttctta atatttgggc
    20641 aatcagtgcg atgtacttta ataccgtgac ctttggtgat ataacctaca atatcatcac
    20701 ctggtatagg attacaacat tttgacaact tgataagtac attttctaaa ccttctacat
    20761 agacaccact atcagtaatg atgttgtctt taataggcaa tgatttcgta acttcttgtg
    20821 cttcatttaa agcacgttgt ttatctaaaa tacgttgtct ttcagttaat ttattaacaa
    20881 tctgtaagga tgtcacgccg ccaaatccta cagctgcgaa taaatcatct tcatttgcaa
    20941 agttatattt ttcattaaca acctgaatat ttttctctgt caaaatatct tcgactctaa
    21001 atccttgctc ttttatttca acttcaacca tcattcggcc tttttcaata ttagatgaac
    21061 gatcttgttt tttgaagaaa cttttaattt tacctttggc actagacgat ttaacaattt
    21121 tcaaccaatc acgacttggt ccatatgaat gtttactagt acgtatttca acaatatcgc
    21181 ctgtttgtaa aatatagtca attggtacaa ttttgccatt caccttggca ccaatcatct
    21241 tattacctac ttcactgtga atcgcataag caaaatcaat cggcacagca ccatatggca
    21301 actcaataac atcactcgct ggggtaaatg cgtatacttt gtcactctgt aagtcatatt
    21361 ttaaggtttc cataaattct tgagcgtcag acgatgtatg atccgcttca gctaattctt
    21421 ttaaccaatt taacttattt tgataagttt gatctttttc acttactttt ttaccttctt
    21481 tgtaagccca gtgtgctgca acaccatgct cagcaatttc gtgcatatca aacgttcgta
    21541 tttggatttc gagcgggtct ccatttggac ctactactgt agtatgcaat gactgataca
    21601 aattttgttt aggcattgca atataatctt taaaacgtcc tggcatcggt ttccataacg
    21661 tatgcaccaa cccaagtatc gcataacaat cattaataga attgacaata acacgtatcg
    21721 ccaacaaatc aaaaatttga tcaaattgtt ttttctgctt catcattttc cgataaatac
    21781 tgtaaatatg tttaggtcta ccatttatat cgccttcgat attcattcgg tccatttcag
    21841 tacgtattct atcaatagcc gtttcgatat acgcttcacg ttcactacgt ttcttcttca
    21901 ttaaattgac tattctaaaa tattgcacat tatcaatata acgaagagcc gtatcttcta
    21961 gttcccattt aattgtatta ataccaagac gatgtgctaa aggtgcataa atttctaatg
    22021 tttctcgaga aattctaatt tgtttttcac gcggcatggc tttcaaggta cgcatattat
    22081 gtaatctgtc tgctaatttc accaaaatta cgcgtacatc tttggcaatc gcaataaata
    22141 acttgcgatg attttcagct tgttgttctt cttttgagcg gtattttact tttttaagct
    22201 tcgtcacacc atcaacaatt cgagcaactt cttcattgaa catttctttt acatcttcaa
    22261 atgtatacgg tgtatcttca attacatcat gcaaaaaacc tgcgacaatc gtcggtccgt
    22321 ctaatcgcat ttctgttaaa atacctgcaa cttgtatagg atgcataatg tatggtaatc
    22381 cgttttttcg gaactgacct ttatgtgctt cataagcaat atgatagctt tttaaaacat
    22441 actcatattc atctgctgac aaatatgatt ttgctttgtg aagaacttca tctgcactat
    22501 atggatattc gttgttcatt atatgataca ccccattcat atttattact tcgccttaaa
    22561 caatgattta ggtactcttg ttgaatagta tttgtcccac accaatcata cgtccgtcga
    22621 cgataaatat ttatcctgtc gtcattaatc gtaatattaa ttttacttga gcgagtttaa
    22681 tttgtatact attcctactt ttaaaacttt tacaaaaatt cgacctaaat ctactgtttc
    22741 attt
//
LOCUS       DAMZLE010000030        22614 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMZLE010000030 DAMZLE010000000
VERSION     DAMZLE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.030"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(659..1519)
                     /gene="folD"
                     /locus_tag="POB92_001035"
     CDS             complement(659..1519)
                     /gene="folD"
                     /locus_tag="POB92_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDI6578544.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1720..2202
                     /gene="purE"
                     /locus_tag="POB92_001036"
     CDS             1720..2202
                     /gene="purE"
                     /locus_tag="POB92_001036"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI6578545.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2189..3313
                     /gene="purK"
                     /locus_tag="POB92_001037"
     CDS             2189..3313
                     /gene="purK"
                     /locus_tag="POB92_001037"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI6578546.1"
                     /translation="MSFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3317..4021
                     /locus_tag="POB92_001038"
     CDS             3317..4021
                     /locus_tag="POB92_001038"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI6578547.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            4021..4284
                     /gene="purS"
                     /locus_tag="POB92_001039"
     CDS             4021..4284
                     /gene="purS"
                     /locus_tag="POB92_001039"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDI6578548.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4286..4957
                     /gene="purQ"
                     /locus_tag="POB92_001040"
     CDS             4286..4957
                     /gene="purQ"
                     /locus_tag="POB92_001040"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDI6578549.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNKKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4950..7139
                     /gene="purL"
                     /locus_tag="POB92_001041"
     CDS             4950..7139
                     /gene="purL"
                     /locus_tag="POB92_001041"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDI6578550.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7118..8602
                     /locus_tag="POB92_001042"
     CDS             7118..8602
                     /locus_tag="POB92_001042"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI6578551.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8595..9623
                     /locus_tag="POB92_001043"
     CDS             8595..9623
                     /locus_tag="POB92_001043"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI6578552.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDSSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIMKNES
                     TAIELLGV"
     gene            9626..10192
                     /gene="purN"
                     /locus_tag="POB92_001044"
     CDS             9626..10192
                     /gene="purN"
                     /locus_tag="POB92_001044"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI6578553.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVSLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10207..11685
                     /gene="purH"
                     /locus_tag="POB92_001045"
     CDS             10207..11685
                     /gene="purH"
                     /locus_tag="POB92_001045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI6578554.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11707..12954
                     /locus_tag="POB92_001046"
     CDS             11707..12954
                     /locus_tag="POB92_001046"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI6578555.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFAIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKNG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13219..14025)
                     /locus_tag="POB92_001047"
     CDS             complement(13219..14025)
                     /locus_tag="POB92_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6578556.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(14018..15418)
                     /locus_tag="POB92_001048"
     CDS             complement(14018..15418)
                     /locus_tag="POB92_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578557.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15433..16008)
                     /locus_tag="POB92_001049"
     CDS             complement(15433..16008)
                     /locus_tag="POB92_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6578558.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16085..16185)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16592..16726)
                     /gene="graF"
                     /locus_tag="POB92_001050"
     CDS             complement(16592..16726)
                     /gene="graF"
                     /locus_tag="POB92_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDI6578559.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16878..18173
                     /locus_tag="POB92_001051"
     CDS             16878..18173
                     /locus_tag="POB92_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578560.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            18598..19770
                     /locus_tag="POB92_001052"
     CDS             18598..19770
                     /locus_tag="POB92_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDI6578561.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19824..20366
                     /locus_tag="POB92_001053"
     CDS             19824..20366
                     /locus_tag="POB92_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDI6578562.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            20520..20786
                     /locus_tag="POB92_001054"
     CDS             20520..20786
                     /locus_tag="POB92_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDI6578563.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20789..22507
                     /gene="ptsP"
                     /locus_tag="POB92_001055"
     CDS             20789..22507
                     /gene="ptsP"
                     /locus_tag="POB92_001055"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6578564.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT         7926 a         3281 c         4248 g         7159 t
ORIGIN      
        1 ataaataatg aggaaataat tattattttg aaaatctgcc acaattgtaa aattaataat
       61 ttagggataa tttgaaatca aatataactt tattcgacag tttgaactta aatttttgtg
      121 tttcaaaaca ataagaaaag ggaggcgaaa atgataaaga accactaatg attaactatg
      181 tagtagtact ttatcatagt cacagcttct gtgatggggc ccccacccca actcgcattg
      241 cctgtagaat ttcttttcga aattctctgt gttgggaccc ctccccaact tgcacattat
      301 tgtaagctga cttttcgtca gcttctttgt tggggcccac accccaactc acattgcctg
      361 tagaatttct tttcgaaatt ctctgtgttg gggcccctga ctagaattga aaacttgtta
      421 caagcttatt ttcattcaat taactactgt ctatataaaa ttgttgagcc aagaacaaag
      481 tgaaatgctc ccttcaaagc cttcatttat tcaatgtcta ctttgaaggg agcatttgat
      541 cgattaatgt cccagatttt tcttgttatt catttcaatt gcgaaataat attaaatgtg
      601 ggttaaaaga cttcattaaa actcagttat taattctcaa tacataacta atgtaaattt
      661 acgaatcaat acctcgacgc attttttctg caagcaaagt attatttaat accatagtaa
      721 ttgttaatgg accaacgcca ccaggaactg gtgtaatagc tccagcaatt tctttaaccg
      781 catcataatc aacgtcacct tttaatttgc cattttcatc tggcgtattg ccaacatcga
      841 taattactgc tccttctttg accacatctt ttgttactaa accaggctta ccaactgcac
      901 tgacaatgac atcagcatct tttaaatatg atgccatatc ttttgaacga gaatgtaaga
      961 ttgttactga tgcatttttt tgaagtagta acttagaaac tggttgtccg acaatatgac
     1021 ttcgtccaat tacaactgca tttttacctt ctaaatcaat atctgcatgt tttaatattt
     1081 ccatgatgcc gagcggtgtg caaggtacaa aagtttgttc atcgatatat aatttcccta
     1141 tatttattgg atgaaatccg tccacatctt tttcaggatt gattgcttct aatattttct
     1201 gttcgctaac ttgttttggt aatggtactt gtaccaaaat accacttaca gaatcatcat
     1261 tatttagtct atttagttcg tttaatactt cttcttcagt agctgtttct tccaaatgta
     1321 cgatttctga aatcatacca attttttcag ctgctttctt ttttgatcta acataacttt
     1381 gactagcgcc atcattacca actaatataa cggataattt aggtgtaaaa cccttttctt
     1441 ttagcgcttc aacttgatct tgtaacccct gtctgtagtc tttggcaatt tgtttaccat
     1501 ctaaaatttt agcaaccata aaaacttctc ctcctattgt caatatccta actttgttag
     1561 ttcataaaac taatttacat agcaaaagac gtaaattcaa acaaaaaatc aaaataaagt
     1621 tcgatttttg attgaaaaag cagaaattgc ttgttatgct atatctataa tatacaacta
     1681 aagtaattca caattcaaac ttttgaaagg gtgtacaaag tgaaagtagc agtcattatg
     1741 ggcagttctt ccgattggaa aattatgcaa gagagttgta acatgttgga ttattttgaa
     1801 attccgtacg aaaaacaagt agtatccgca catcgtacgc caaaaatgat ggttcaattt
     1861 gcttctgaag cgagagaaag aggtataaac attatcattg caggcgctgg cggtgcggca
     1921 catttaccag gtatggttgc atcattaacg acgctaccag ttattggtgt gccgattgaa
     1981 acaaaaagtt taaagggtat agattcttta ttatcaattg ttcaaatgcc aggaggtatt
     2041 ccggttgcaa cgactgcaat tggtgcagca ggtgctaaaa acgcaggtat acttgcagca
     2101 agaatgttaa gtattcaaaa tccttcttta gttgaaaaac taaatcagta tgaatcttcg
     2161 ttaattcaaa aagtggagga catgcaaaat gagcttcaat aaattaaagt ttggtgcgac
     2221 tattggcatt attggtggtg gtcagcttgg aaagatgatg gcacaatcag ctcaaaaaat
     2281 gggttataaa gtggttgtat tggatccttc tgaagattgt ccatgtagat acgttgcaca
     2341 cgaatttata caagccaagt atgacgatga aaaggcactc aatcaattag gacaaaaatg
     2401 tgatgtgatt acttatgaat ttgaaaacat ttcagcccaa caattaaaac tattatgtga
     2461 aaagtacaat attccgcaag gttaccaagc tatacagtta ttacaagacc gcttaactga
     2521 aaaagaaaca ttaaaaagtg ctggtaccaa agttgtcccg ttcatttcag taaaagaatc
     2581 tacagatatt gacaaagcaa ttgaaacatt aggatatcct tttattgtaa aaactagatt
     2641 tggtggctac gatggcaaag gtcaagtttt aattaacaac gaaaaagact tacaagaagg
     2701 ttttaaatta attgaaacta gtgaatgcgt agctgaaaaa tatttgaata tcaagaaaga
     2761 agtatctctt actgttacaa gaggaaacaa caatcaaatc acttttttcc cattacaaga
     2821 aaatgagcat agaaatcaaa tacttttcaa aacaattgtt ccagcgagaa tagataaaac
     2881 agctgaggcg aaagagcaag ttaataaaat tatccaatcg attcatttca ttggaacatt
     2941 tacagttgaa ttttttatag atagtaacaa ccaattgtat gtgaacgaga tagcaccaag
     3001 gcctcacaat tccggacatt attcaattga agcatgtgat tattcacaat ttgatactca
     3061 tattttagca gttaccggac aatcattacc aaattcaatt gaattattaa agccagcagt
     3121 catgatgaac ttactaggta aagatttaga tttattggaa aatgaattta atgaacatcc
     3181 agagtggcac ttacatattt atggtaagtc tgagcgtaaa gatagcagaa aaatggggca
     3241 tatgactgta ctaacgaatg atgtaaacca aactgaacaa gatatgtacg ctaaatttga
     3301 ggggagtaat taaagcatga cattattata tgaaggaaaa gcgaagcgca ttttctcaac
     3361 aaatcaagaa aatgaattaa gagttgaata taaagatgaa gttactgctg gaaacggggc
     3421 taagaaagac acaatggcag gtaaggggcg attaaataat caaattactt ctattatatt
     3481 taaatattta caagaaaatg gaatagaaag tcactttatt aaacaattat ctgaaacaga
     3541 acaattagtt aagcctgtga aaataattcc attagaagta gttgttcgta atattgctag
     3601 tggatctatt acaaagcgtt taggttttga aaatggtgaa gtttttagag aaccacttgt
     3661 agaatttttc tataaaaatg atgcgttaaa tgatccgttg ataacggatg accatgttaa
     3721 attgctcaat atagcatcag atgaagatat tgaaatacta aaatccaaag cattaaagat
     3781 taataatgtg ttgaaacaat taatggatgc tatgaattta aaattagtag attttaaaat
     3841 cgaatttgga aagactgaga ctggtcaaat tttgttagcg gatgaaatat ctccagatac
     3901 atgtcgaatt tgggataaag ctaccaatgc aaactttgat aaagatgtat atagaaataa
     3961 cactggatca ctgattgaaa catatcaaat atttttaaac aaattggagg atttaaaata
     4021 atgaaaacaa ttgaactaca tatcacatta caaccacaag tattagatac gcaaggacaa
     4081 acgcttactc gagctgtaca tgacttaggt tatgcacaag tgaatgatat tcgtgtagga
     4141 aaagtattat atatgacagt ggatgaggtt agtgatgaaa aggtacacaa cattattaca
     4201 actctaagtg aaaaattgtt tgcaaataca gtgattgaag aatatagcta taaagtgtta
     4261 gatgatgaaa aggagaatgc ataaaatgaa atttgcggtt cttgtttttc caggttcgaa
     4321 ttgtgataga gacatgttta atgctgctat taaaagtggt gttgaagcgg aatatgtaga
     4381 ttatagagaa acatcactaa gtggatttga tggcgtactt attcctggtg gattttcatt
     4441 cggggattac ttaagatctg gggcaatggc tagtgtagcg ccgattattt cggaagttaa
     4501 acgtcttgca gctgaaggta agccagtatt aggtgtttgt aatgggtttc aaattttaac
     4561 tgaaataggc ttattacctg gtgcattatt gcataacgat tcacatttat ttattagtag
     4621 aaatgaagag ttagaaatag tgaataatca aacggcattt acaaatcttt atgaacaagg
     4681 tgaaaaagtt atatatcctg tagctcacgg tgaaggtcat tattattgta ctgatgaaat
     4741 atatcaacaa ttaaaagcta acaatcaaat tattctgaaa tatgtgaata atccgaacgg
     4801 ttcatatgat gatattgcag gaattgttaa caaaaaaggc aatgtatgtg gcatgatgcc
     4861 acatcctgaa agagctttag aaacgttgtt aggtactgat agtggtgtga aattatttga
     4921 agcgatggta aaaagttgga gggaacaaca tgtctaaatt tatcgaacca agcgttgaag
     4981 aaattaaact tgaaaaagta tatcaagata tgggattaag tgatcaagaa tatgaaaaag
     5041 tttgcgatat tttaggcaga caacctaact ttacagaaac aggtatcttt tctgttatgt
     5101 ggagtgaaca ttgctcttat aaacattcta aaccgttttt aaagcaattt cctacgtcag
     5161 gtgaccatgt gcttatgggg cctggtgaag gtgcaggggt agtcgatata ggtgataatc
     5221 aagccgtagt atttaaagta gagtctcaca atcatccatc agcaattgaa ccatatcaag
     5281 gggctgctac aggcgttggt ggaatcattc gtgacattgt ctctattggg gctagaccta
     5341 ttaatttgtt aaacagtctt agatttggag aattagataa taaacaaaac caaagattac
     5401 ttaaaggtgt tgtaaagggt atcggaggtt atggtaactg cattggtatt ccaacaactg
     5461 ctggtgaaat cgaatttgat gaacgttatg atggcaatcc acttgttaat gcaatgtgtg
     5521 ttggtgttat caatcacgac atgattcaaa aaggcacagc aaaaggtgta ggtaattcgg
     5581 tcatttatgt tggtttgaaa actggtcgag atggtattca tggtgctact tttgcatctg
     5641 aagaattgac ggaagaaagc gaaagtaaac gaccttctgt acaaatcggt gatccatttg
     5701 taggtaaaaa attaatggaa gcaacacttg aagcaattac atttgatgaa ttagttggta
     5761 ttcaagatat gggtgctgct ggtttaacat cttcatcgtc tgaaatggcg gcaaaaggtg
     5821 gtagtgggtt acatttgaga ttagaacaag tgccaacacg tgagccaggt atttctcctt
     5881 atgaaatgat gctttcagaa actcaagaac gtatgttact agttgttgaa aaaggtactg
     5941 aacaaaaatt cttagattta tttgataagc acgaattgga tagtgctgtt ataggtgaag
     6001 ttacagatac aaatcgtttt gttttaacat atgatgacga agtttatgct gacattccag
     6061 ttgaaccact agctgatgaa gcacctgtat atattttaga aggagaagaa aaagattata
     6121 atacttctaa aaatgattat acacacatcg atgttaaaga tactttcttt aaattactta
     6181 agcatccgac tatagcatct aaacactatt tatatgatca atacgaccaa caagttggtg
     6241 ccaatacgat aattaagcca ggacttcaag catcggtagt acgtgtggaa ggcacaaata
     6301 aggcaattgc ttcaacaatt gatggtgaag cgcgttatgt atataacaat ccatatgaag
     6361 gtggaaagat ggtagtagct gaagcttatc gaaatttaat tgccgtgggt gcaacaccat
     6421 tagcaatgac agattgttta aattatggtt ctcctgaaaa gaaagaaatc tatcaacagt
     6481 tgatagattc aacgaaaggt atggcagaag catgcgacat tcttaagaca ccagtagttt
     6541 ctggtaatgt atctttatat aacgaaacga aaggtacttc tattttccca acaccagttg
     6601 ttggaatggt aggtttgatt gaaaatgtaa attatttaaa tgattttgaa cctcaagttg
     6661 gagataaatt atatttaatc ggtgatacta aggacgactt tggtggtagt caacttgaaa
     6721 agttaattta tggcaaagtt aatcatgaat ttgagtcatt agatttgagt tcagaagttg
     6781 aaaaaggtga atcaatcaag accgctattc gtgaaggact attatcacat gttcaaacag
     6841 ttggtaaagg tggcttactg attaccttag ctaaactaag tgcgcattac ggtttaggat
     6901 taaaatcttc aatagatata acaaatgcac aattgtttag tgagacgcaa ggccgatatg
     6961 ttgtttctgt taaatcaggt aaaactttaa atattgataa tgcaatagaa attggacttt
     7021 taacagatag tgataatttc aaggtaacaa caccatatac agagattagt gaaaatgttt
     7081 cagatattaa acaaatatgg gaaggggcaa ttgctcaatg tttaactact caggattaaa
     7141 cgaagaatgt ggcgtgtttg gtatttggaa tcatcctgaa gcagcgcaac taacatatat
     7201 gggacttcat agtttgcaac atcgtggtca agaaggtgca ggtatagttg tttctgatca
     7261 aaatgaatta aaaggcgagc gaggattagg cttactaact gaagcgatta aagatgatca
     7321 aatggaacga ttaaaaggat atcaacatgc aattggtcac gtccgttatg ctacttcagg
     7381 taataaaggt attgaaaata ttcaaccgtt tctgtatcac ttttatgata tgagtgtagg
     7441 tatttgtcat aatggtaacc tcattaatgc taaatcattg cgtcagaatt tagaaaaaca
     7501 aggtgctatc ttccattcgt cttctgatac tgaagtcatt atgcatttga tacgtcgaag
     7561 taaagctcct acttttgagg aagcgttgaa agaaagtttg cgaaaagtta aaggcggttt
     7621 tacatttgcg attttaacta aagatgcgtt atatggcgca gtagatccaa atgctatcag
     7681 accacttgtt gtaggtaaaa tgaaagatgg gacatacatc cttgcaagtg aaacatgtgc
     7741 aatagatgtg ttaggtgcag aatttgttca agatattcat gcaggtgaat atgtcgtgat
     7801 taacgataaa ggtattacag ttaaatctta tacacatcat acgacaactg caatttctgc
     7861 gatggaatat atttattttg ctagaccaga ctcaacaata gctggtaaaa atgtccatgc
     7921 agtacgtaaa gcttctggta aaaaattagc ccaagaaagc cctgtaaatg ctgatatggt
     7981 catcggtgta cccaattcat cgctatcagc tgcgagtggt tatgctgaag aaataggttt
     8041 gccatatgaa atgggactag ttaaaaatca atatgttgca agaacattta ttcaaccaac
     8101 tcaagaatta cgtgagcaag gtgtgagagt gaagttatct gcggtaaaag atatagtaga
     8161 tgggaaaaat atcattcttg ttgatgattc cattgttcgc ggtacgacaa ttcgacgcat
     8221 tgtgaaaatg ttaaaagatt ctggtgcaaa taaagtgcat gtgcgtatag catcaccgga
     8281 atttatgttc ccaagttttt atggaatcga tgtttcaact acggcagaat taatttctgc
     8341 aagcaaatca cctgaagaaa ttaaagatta tattggcgct gattcattag catatctatc
     8401 tgtagatggg ttaattgaat caattggttt agattatgac gcgccatata gtggcttatg
     8461 tgtagaaagt ttcactggag attatcctgc agggttatat gattatgaag caaattataa
     8521 agcacattta agtcatcgac aaaagcaata tatttctaaa aacaaacact tttttgatag
     8581 cgagggaaat ttaaatgtct aaagcatatg aacaatctgg tgtaaatatt catgctggtt
     8641 atgaagctgt agaaagaatg tctagtcatg ttaaacgtac gatgcgtaaa gaagttatcg
     8701 gtggtttagg tggattcggt gctacatttg atttatcaca attaaatatg acagcgccag
     8761 ttttagtttc tggaacagac ggagtaggta cgaaattaaa actagctatc gactatggga
     8821 aacatgattc gataggtatc gatgcagtcg caatgtgtgt taatgatatt ttaacgacag
     8881 gtgcagaacc attgtatttt ttagattaca tcgctacaaa taaagttgtt cctgaagtta
     8941 ttgaacaaat tgttaaaggt attagtgatg catgcgttga aacgaatact gcacttatcg
     9001 gtggagagac tgctgaaatg ggtgaaatgt atcacgaagg tgaatatgat gtagccggat
     9061 ttgcagttgg agcagttgaa aaggatgact atgtagatag ttcagaagtg aaagagggac
     9121 aagttgttat agggcttgcg tcaagtggca ttcattcaaa tggatatagt ttagtgcgca
     9181 aattaattaa tgaatcaggc attgatttgg catcaaactt cgataatcgt ccatttatag
     9241 atgtcttttt agaaccaact aaattatatg tcaaacctgt acttgctttg aaaaaagaag
     9301 tttctattaa ggcaatgaat catattactg gtggaggttt ttatgaaaat attccacgtg
     9361 cattgccagc cggatatgct gctagaattg atactacatc atttccaacg ccaaaaatat
     9421 ttgattggtt acaacaacaa ggcaatatag acacaaatga aatgtataac atttttaaca
     9481 tgggtattgg ctatacggtt atcgttgatg aaaaagatgc atcacgcgct ttgaagattt
     9541 tagcagaaca aaatgtggaa gcctatcaaa ttggtcatat tatgaaaaat gagtcaactg
     9601 caattgaatt gttgggggta taacaatggt taaaattgcg atttttgcat caggttcagg
     9661 aagtaacttt gaaaatatag ttgagcatgt tgaatcagga aaacttgaaa atattgaagt
     9721 tacggcgcta tatacggatc atcaaaatgc gttttgtata gatagagcaa aaaagcacga
     9781 tattcctgtt tatattaatg aaccaaaaca atttgattca aaagcagcgt atgaacaaca
     9841 tttagtatca ctattaaatg aagataaggt agagtggatt attttagctg gctacatgcg
     9901 tctaattggt ccagatttat tagcttcatt tgaaggtaaa atattgaata tacatccatc
     9961 tctattgccg aaatataagg ggattgacgc aataggccaa gcatatcata gtggcgatac
    10021 tattactggt tcgacagtac attatgttga ttgtggtatg gatacgggag aaattattga
    10081 acagagacaa tgtgatatta gaccggacga ttcaaaagaa caattagaag agaaagtaaa
    10141 aaaattggaa tatgagttat atccaagtgt tattgctaaa attgtaaaat aaggagtatg
    10201 tatgtgatga agaaagctat tttgagcgta tcaaataaaa caggtattgt agagtttgct
    10261 aaagcgttaa cgcaattaaa ttatgaatta tattcaacag gtggtactaa acgtatatta
    10321 gatgaagcaa atgtaccagt tcgttctgtt tcagacttaa cacattttcc agaaataatg
    10381 gatggccgtg ttaaaacatt acatccggct gttcatggtg gcattttagc tgatcgaaat
    10441 aaaccgcagc atttaaatga attatcagaa caacatatag atttaattga tatggtagta
    10501 gttaatttat atccattcca acaaactgtt gcaaaccctg atgtgacgat ggacgaagca
    10561 attgaaaata ttgatattgg tggtccaaca atgttacgtg ctgcagctaa aaactataaa
    10621 catgtaacaa caattgtaca tccggcagat tatcaagaag tattgacgcg attaagaaac
    10681 gattcgttag atgagtcata tagacaatca ttaatgatta aagtttttga gcatactgca
    10741 gaatatgatg aagcgattgt acgtttcttt aaaggggata aagaaacttt aagatatgga
    10801 gaaaatccac aacaatcagc gtattttgtg agaacttcga atgctaagca cacgattgca
    10861 ggcgctaaac aattacatgg gaaacaatta agctataaca atattaaaga tgcagatgct
    10921 acactagctt tagttaaaaa gtttgataca cctgctacag ttgcggttaa acacatgaat
    10981 ccatgtggtg ttggtatcgg tgacacgata gaacaagcat ttcaacatgc gtatgaagcg
    11041 gatagtcaat caatatttgg tggaattgtt gcattaaacc gagctgtaac acctgagtta
    11101 gcagagcaat tgcatagtat ctttttggaa gtcattattg caccaaaatt tacagatgaa
    11161 gcattagata ttttaaaaca aaagaagaat gtaagattat tagaaattga tatgactata
    11221 gacagtaacg aagaagagtt tgtttcagta tctggcggat atttagttca agataaagac
    11281 aattatgtcg tgccaaaaga agaaatgaaa gttgttacag aagtagcacc tactgatgaa
    11341 caatgggaag caatgttatt aggatggaaa gttgtaccat cagtaaaaag taatgcaatt
    11401 attttaagta ataataaaca aactgtaggt ataggtgctg gacaaatgaa tcgtgtcggt
    11461 gctgctaaaa ttgcgttaga gagagctatt gaaatcaatg atcatgtagc gttagtatct
    11521 gatggatttt tccctatggg agatacagtt gaacttgcag cacaacatgg tataaaggca
    11581 attatccaac cgggtggttc gattaaagat caagattcaa ttgatatggc taataaacat
    11641 ggtattgcaa tggtagtcac aggcactcga cattttaaac actaatttta aaggggcgta
    11701 acataaatga atgtattagt aattggtgct ggtggacgag aacatgcact tgcatataaa
    11761 cttaatcaat cgaatctagt taaacaagtg tttgccattc caggtaatga ggcaatgaca
    11821 cctatagctg aagtacacac tgaaatttca gaatctgatc atcaagcgat attagatttt
    11881 gctaaacggc aaaatgttga ttgggtagtt ataggtccag aacagccgct aattgatgga
    11941 ttagcagaca ttttacgagc gaatggtttc aaagtgtttg gtccaaataa gcaagcagct
    12001 caaatcgaag gctcaaaatt atttgctaaa aagataatgg aaaaatataa tattccaact
    12061 gctgattata aagaagttga gcgaaaaaag gatgctttaa catatattga aaactgtgaa
    12121 ttgcccgttg ttgtcaagaa agatgggtta gctgctggga aaggcgttat tattgcagat
    12181 actattgaag cagccagaag tgctattgag attatgtatg gtgatgaaga agaaggtact
    12241 gttgtatttg aaacgttttt agaaggtgaa gagttctcgc taatgacatt tgttaatggt
    12301 gatttagcag tacctttcga ctgtattgca caagatcata aacgcgcatt tgatcatgat
    12361 gaaggaccaa atactggtgg tatgggggct tattgtccag taccacatat tagtgacgat
    12421 gttttaaaac ttacaaatga aacaattgca caacccattg caaaggcaat gcttaatgaa
    12481 ggttatcaat tcttcggtgt attatacatt ggtgctattt taactaaaaa tggtccaaaa
    12541 gtaatagaat ttaatgcccg ttttggtgat cctgaagctc aagtattatt aagtcgcatg
    12601 gaaagtgatt taatgcagca tattattgat ttagatgaag gaaaacgtac tgaattcaaa
    12661 tggaaaaatg aatctattgt aggggtcatg ttggcatcaa aaggatatcc tgatgcatat
    12721 gaaaaagggc ataaagtaag tggctttgat ttaaatgaaa actattttgt tagtggatta
    12781 aagaagcaag gtgatacctt tgttacttca ggtggtagag ttatacttgc catcggaaaa
    12841 ggtgacaatg tacaagatgc acagcgagac gcatacgaaa aagtatcaca aatacaaagt
    12901 gaccatttat tctatcgtca tgacattgcg aataaagcac tacaacttaa ataagtaaat
    12961 ttaaaatact aagattagct atgaacgaat ctataacgat agattttttc atagcttttt
    13021 tagttgtaga gtctaggaca ttgatttctg taccaaattt gtgattatgc atatgtaata
    13081 caaaagaggc gccacaacat gtttggatga acaaaataac atgtttgtgg cacctctttt
    13141 gtttagtatg gaataaatgg ttttcttttt ctatacaatg aatttctaat ttagtatcta
    13201 tacaattatg gataaaattt aacctacacg accaagacga acatcatcta tgcccgtgat
    13261 gggtaaggtg attgaacaat aatatgccaa agtaataatg gcaattaaaa ctataataaa
    13321 gattatatct ttatatgaga aaggtacgtt gtaatagtaa gtacgaggac catctctaaa
    13381 tcctttggac tccatcgcaa ctgataattg atgtgccttt ctaatatttt ggcttaatag
    13441 aggtataatt aaatgcttaa atcgctttaa ccctctataa tttgctgcgt ctatcatctg
    13501 atagcgcatt tttaaagatc tgcgaagctg tattaaagaa ctaatcatta aaggtatcat
    13561 acgaatggca gccatgaatg cataagcaac ttttgattta acctttaaat gttgcattaa
    13621 actataaaat atcatgacaa cctgagatgt aagtgcgatt aagataccga aaaatgaaat
    13681 agcaatggtt cttaatgata catgtaaacc acgaactaaa ctttctgttg taatatggat
    13741 aaatccgaat ttcaaaattg tatggctacc attcccgtat aaaatcatga acagggaaga
    13801 gagtaatgca aagccaatac ttatagttat aaaaattgct gtaattttaa actgagtacc
    13861 attaaacatc aataagaaaa ctaacattaa gatagtgata taaagcataa aatcgaaatt
    13921 atgcacaaat ataataaaga aaaatagtat aattccaaga aatagtttcg ttataatgtt
    13981 gacatcatca acaaatgatt gccgaacttt ccattgctca tacattcgta tcaccatcac
    14041 aatctagtaa cgcaccatct gaaattttaa gtcttcttga tggataacgt tcaattattt
    14101 catcgtcatg tgtaaccatg acaatacttt gtcccaaatt aattcgcttt tggaaaagtt
    14161 tgatcaactg gaatgtatta tggctatcaa gtccaaatgt cggttcatct aaaaagataa
    14221 tatcagcttt agaacttagt gcggtagcta cgctaaggcg tcgtttttga ccaatagaca
    14281 actcataagg atgttgatct tttacatttt gtaaatctaa aagttttaaa agttgtatcg
    14341 tttcatcatc actttgatct ttagaaaggt gattaaaatg aatgttaatt tcatcataaa
    14401 ccgaatttgt tataaattgt aattctgggt tttgataaac taggtacatg tgttttgctg
    14461 catgtttaat ttttgttaaa cgctgatttt caaaataaac atcaccttga tatttaatca
    14521 attgcataat tgattcaagc aaggttgttt taccactacc atttgcccct gtaattgtaa
    14581 tccactcacc tagaccaatt tctaaatctg agaatgagag caatgttgat ttaccgcgaa
    14641 taatacgtcc atttttaaat tgtaataagt gtgagtttgt tgttggaaag tcaacacgac
    14701 ttggtgcgaa ttcccatgca cgtggatgcc acacaccata ttcactgagt aaatgaacat
    14761 acttctgtaa tatgatttca ggacattcat cggcaatgat atttccgtta taatccatca
    14821 aaatgacgcg gtcgacatga ttccagatgt gtttaacttt atgttcaacg attacaacag
    14881 tttgatcttc ccaaagttca attagtttag tccataaatc ttctgttgct tgaacatcta
    14941 acattgctgt cggttcatct aaaaacaatg tttttgattg ttgaagaatg gtttcaacaa
    15001 ttgccaattt ctgtttcatc ccgccactta aatctttgat atacgtttca ggggtaacat
    15061 ttaaattgac catatttaaa gcattgataa ttaacgcatc catgtcttca cgtggtactt
    15121 gtctattttc taaaacgaat gcaagttctt cgtatacttt tggcatacaa aactggctat
    15181 cagggtcttg gaaaataacg ccacttaatg ggtcaacgat tagttcatca tatttcatag
    15241 gtaattcaat taaattagga acaataccac ttaatacatt cagaagtgta cttttaccgc
    15301 aaccagaagg accgagtaaa agtacttttt ctttgtcttg aatagtgata tttaaatgat
    15361 cgaaaatttt acgctgacca cttggatatt ttaatcgtaa atcacttact tttaacactc
    15421 tgaatgctcc tcttataagt tgtcgtaatc ttctttagca gctggtctaa ataattttgt
    15481 tacgcctgtc ttatctaaag cttttactaa aaggtaagat aggacgccgg cgactactgc
    15541 accactaatt aatctaaata cgatgaataa tgttaagttc caacctgcaa cttcatttaa
    15601 ataaccatag aaataatcta tagggaaagc cgcgattgct gtacaaaaac ctgctaacat
    15661 agctaccata actgaacgag attgatattt aaaaattgca aagacaagtt cacacgctaa
    15721 accttgtatg aaagcgtaaa cgattgtcgg aatatcgaaa cgacccataa tgatagtttc
    15781 gccggcacct gcagcaaatt cagccagtaa agcaatacct ggttttggaa taattagata
    15841 gcagacaatc gctgccatga accaaacccc gtttgttaat tgttcgaggt gaaggcctgt
    15901 agcttgcaca ccattgtaaa caaaccacca taaattgtaa ataactgcga atactactga
    15961 aataagtacg gttactagta tttcagatag ctttaaacct tttgacattt ttacatcctc
    16021 ctaataaaaa aacgcacaac catccatagg aaagttatgc gttcacaata tatattagta
    16081 aaacatatgt atagtaacac tttcctacgc tagttcaagc tagatcaggt tcaaagggtt
    16141 tgagggcaag cctcatctca gtataaaaca cccctagtgt gtgcgattta tttaattaat
    16201 tatactgtaa gacgtttgta aacttatgtc aataggttgt cttcatgaaa ttttgtttaa
    16261 ttcgatttaa aatttataat attagcattg gatttaaatt gaagatgtag taggaatgtt
    16321 agtaattaaa gatataaaaa tatgtgacat gtaataagat tgagctgata aatgaagagg
    16381 gatacttatc aatcatacct ctttaacaac agtgaagaac ccgtgcataa tggcttacga
    16441 attatagttt gtagggtaaa agagggatac atgcgccgag cacatgcaaa aaagccccta
    16501 acaactaaaa gttgtaagga agaagaggga cacatgcgcc gagcacatgc ataaaatccc
    16561 ctaacaacta aaagttgtaa ggggatttaa attaatttag tgtatcttgg atatcttgtt
    16621 ttgtttgatt aatatcttct gttttttctt cttttttatc ttttaatttt tcttcaactt
    16681 ctttagcttt ttctgctgct tttttatttt gattttcatt agacatgatt aattcctccc
    16741 aaattggata attatttata tataaatctt acccggttgt actttcgtta aacttttcta
    16801 agtctatagc actatttatt catttatcta aagacaacaa cattagatta atatataatg
    16861 attttgaggt gaacataatg tcttttctta ggaaacacgc cgaaattatt tttagctatt
    16921 taatcggtat cgtttcactc ttcactggtc tcattatttt aattaacttg ccattaatta
    16981 aacaattaaa tggtggtaaa aaagttgata cacatgttca taatgtgtgg gaatttctga
    17041 atgcattttt cagtgaaatt attaaagtaa tgagtcgatt tataggtaat ttccctatag
    17101 ttagtgcaat tgtgataatt atattcggta ttttagttat gttgattggt catacattac
    17161 ttagaactat taagtatgac tatgatattt ctatcttttt cttagttatc ggtatcatgt
    17221 actttattat tactcttata ttaatgactc aagtttatgg attctttgca gtgattttca
    17281 ttattccatt tacaattcat ataggatata tcgtctataa agatgaattg aatcaggaaa
    17341 atgtaaaaaa tcatttcatg tggataattg tgagttatgg tataagttac ttaattacac
    17401 aaattgcatt gtatggcaga attgatgcta atgaaataga gtcaattgat atcttaagtg
    17461 tcaatgcttt ctttataatt atgtggttac ttggtcaaat ggctatttgg aatttcttgt
    17521 tcttgcgccg agctttacct ttaacaaagc aagaattagg tgaagaggag ccagaattat
    17581 caagaacaag taaagggaat gtcacgaatc aaactaaaat tcacttgaaa caactccaag
    17641 ataagactac agaatatgca cgtaagacaa gaagaagtgt cgatttagat aaaattagag
    17701 ctaaaagaga taaattcaaa aagaaagtta atgatattat cgatattcaa gaagacgata
    17761 ttcctgattg gatgagaaaa ccgaaatggg ttaaaccaat gtatgtcgaa ctattttgtg
    17821 gtgtcgtcat ctttttattc acatttttag aatttaataa tcgtaatgca ttatttgtat
    17881 ctggtgattg gaaattatca cagacacaat atgttattga atgggttaca ttactaattc
    17941 tgttattcat tattatcgca tatatcgcta caacgttaac tttccacttg aaaggtaagt
    18001 tttattattt acaactattt atggggagca ttttattctt taaattgtta acggaattca
    18061 taaatataat gattcatgga ctactacttt cagtgttcat tacaccaaca ttactattaa
    18121 tgttattggc aatcatcatt tcttattcgt tacaattacg agagcgacca taattaaaag
    18181 cattataaaa gtactatcta ttaaacattt tgatgtgtac gctataagtt agatatatct
    18241 ctaacttatt tagatacagg tcaatgaagt ttatggatag tacttttttt gtaactagat
    18301 ttgattgatt caggtgatgt gaattaagta ttgaaaattg tatacaaagt tttagtgcaa
    18361 ataaaatagt tgaaaagtta tccatttgta aaatcaagaa aactattaaa tagttgaatc
    18421 gacatttgga aattgcgaaa cgatatatag tatttccttt gtagaaattt aacatatatc
    18481 attcgaatta ctaatttgtt aaaatcaaca ttaagattag aagtagatga tattgaaatt
    18541 tggcaaacaa tttaatctat ataaaactac aacgaaacac agaaaggaag ttgtcagatg
    18601 aaaatagcaa ctctgaacaa aggcaaagaa acaaaatatt ttaatggata tcctttaatt
    18661 gaagaagagg atatctattc acaagatcat ttaaaagaag gagatatttt tcaaattgtg
    18721 actgataaat cacaatatgt tgcaacggct tatgttggtc gccaacataa aggattaggt
    18781 tgggttctaa catacgataa agctcaagaa atcaacacag ctttctttgt gaaattgttt
    18841 aatactgcat tagcagaacg tgattattat tttaatatag atggaacaaa tgcttttaga
    18901 ttatttaatg ctgaaggtga tggtgttggg ggattaacaa tcgacaatta cgatggtcat
    18961 ttgttgattc aatggtactc aaaaggtatt tataaattta aatatgccat tcttgaagcg
    19021 gttagaaaag tatttgatta taaatctatt tacgaaaaag taagatttaa agacagcgaa
    19081 tatagtggtg gttttgttga aggagatgca cctgagtttc caattgttat cgaagaaaac
    19141 ttcacatttt ataatgtaga ccttgaagat ggtttgatga caggtatctt tttagatcaa
    19201 aaagaagtgc gcaagaaatt aagggatcaa tatgccaaag aacgccatgt tttaaactta
    19261 tttagttata caggtgcttt ttctgtaata gcagcaagtg aggcatcttc aacaacaagt
    19321 gtagatttgg ctaatcgttc tcgtagttta actgaagaaa attttggatt aaatgctatt
    19381 gatcctaaat cccaatatat ttatgtcatg gacacttttg atttctataa atatgctgca
    19441 cgacatggac atagttatga cacgatcgtg attgatccac ctagctttgc gcgtaacaaa
    19501 aaacgtacat tttcagtgca aaaagattat gacaaattaa ttaatggcgc cttaaatatc
    19561 ttatcatctg aaggaacatt attgttatgt acaaacgcaa gtgtatatcc attaaagcaa
    19621 tttaaaaata ctattaaaaa gacgcttgaa gagagtggcg ttgattatga attaactgaa
    19681 gttatgggat taccaaaaga ttttaaaacg catccacatt ataagccatc taaatattta
    19741 aaagctgttt ttgtaaatat tagacattaa tttgataaaa aggggtatag taacttattg
    19801 agaaaaagaa gggtgataat attatgggat tcaaaaacaa tttaacatca aatttaacaa
    19861 ataaaattgg taattcagtc tttaaaatag aaaatgttga cggaaaaggt gcaatgccaa
    19921 cgacgattca agaattgaga gaaagacgac aacgtgctga agcaattgta aagagaaaat
    19981 ctttaatgtc atcaacaatg agcgttgttc caattccggg tttagatttt ggtgttgatt
    20041 taaaattaat gaaagatatt atcgaagatg taaataaaat atacggatta gatcataagc
    20101 aagttaatag ccttggggat gatgtgaaag aaagaattat gtctgcagca gcaattcaag
    20161 gtagtcaatt tattggtaaa agaatttcaa gtgcattttt aaaaatagtt attagagatg
    20221 tagctaaacg tactgctgca aaacaaacaa aatggtttcc tgttgtagga caagctgtgt
    20281 ctgcatctat tagttactat tttatgaata aaattggaaa agatcacatt caaaaatgcg
    20341 aaaatgttat taaaaatgtc atgtaggtgc tataatagtt ttgcaatttg caaattttac
    20401 tgaaaccggt tttaaacgaa ttgaatttaa agcatggttt tggtaaagtt aatgtataaa
    20461 actaagttag tattgtaata atattgaaga ttctaactat acgaaggaga aatgtaatta
    20521 tggaacaaaa ttcatatgta atcatcgacg agactggtat tcacgctaga ccagcaacaa
    20581 tgttagtaca aacagcttca aaattcgatt ctgatattca attagaatat aacggtaaga
    20641 aagtaaactt aaaatcaatc atgggtgtta tgagccttgg tgttggtaaa gatgctgaaa
    20701 ttacaattta tgctgacggt agtgatgaat ctgacgccat tcaagcaatc agtgacgtct
    20761 tatcaaaaga aggattgact aaataatcat gtctaaatta attaaaggta ttgccgcatc
    20821 tgatggtgtc gcaattgcta aagcttattt attagttgag ccagacttaa cattcgacaa
    20881 aaatgaaaaa gtcactgatg ttgaaggaga agttgcaaag ttcaatagcg ctatcgaagc
    20941 ttctaaagtt gagttaacta aaattagaaa taatgcagag gttcaactag gtgctgataa
    21001 agctgctatc tttgatgcac atttattagt tttagatgac cctgaattaa ttcaaccaat
    21061 ccaagataag attaaaaatg aaaacgctaa tgctgctaca gcattaacgg atgtaacaac
    21121 acaatttgtt acaatttttg aatctatgga taacgaatac atgaaagaac gtgcggctga
    21181 tattcgcgac gtttctaaac gtgtgttatc acatatttta ggtgtagaat taccgaatcc
    21241 gagtatgatt gatgaaagcg ttgttattgt agggaatgac ttaacgccat ctgatactgc
    21301 tcaattaaat aaagaattcg tacaaggttt tgctacaaac attggcggaa gaacaagtca
    21361 ctctgcaatt atgagtcgtt ctttagaaat tccagcaatt gttggtacaa aatcaattac
    21421 tcaagaagtt aaacaaggcg acatgattat cgtagatgga ttaaatggtg atgtaatcgt
    21481 taatccaact gaagatgagt taatcgctta tcaagataaa cgtgagcgtt attttgctga
    21541 caagaaagaa ttacaaaaac tacgtgatgc tgatactgtt acagttgatg gtgttcacgc
    21601 agagcttgct gcaaatattg gtacacctaa tgatttgcca ggtgttattg aaaatggtgc
    21661 acaaggtatc ggcttatata gaactgagtt tttatatatg ggtcgtgacc aaatgcctac
    21721 agaagaagaa caatttgaag cttataaaga agtattagaa gcaatggacg gtaaacgtgt
    21781 tgttgtacgt actttagata taggtggaga taaagaatta tcatacttaa acttgcctga
    21841 agaaatgaat ccattcttag gttaccgtgc gattcgttta tgccttgcgc aacaagatat
    21901 tttcagacca cagctacgtg cattattacg tgcatcagtt tatggtaagt taaatatcat
    21961 gttcccaatg gttgcaacaa ttaacgaatt tagagaagct aaagctatat tattagaaga
    22021 aaaagaaaac cttaaaaatg aaggtcatga catttcggat gatatagaat taggaatcat
    22081 ggtagagata cctgcaacag cagcattagc tgatgtcttt gctaaagaag tagatttctt
    22141 cagtatcggt acaaatgatt taattcaata cacattagct gctgaccgta tgtcagagcg
    22201 tgtatcatat ctataccaac catataaccc ttcaatctta cgtttagtta aacaagttat
    22261 tgaagcgtca cataaagaag gtaaatggac aggtatgtgt ggtgaaatgg ctggagatga
    22321 aacagctatt ccattattgc ttggtttagg tttagatgag ttctctatga gtgcaacgtc
    22381 tattctgaaa gcaagaagac aaattaatgg tttaagtaaa aatgaaatga ctgaacttgc
    22441 taaccgtgca gtcgactgtg caacgcaaga agaagttatt gaattagtta acaactacgt
    22501 aaaataatta attctttcga aagacagaat tataaataaa cccgaagtat tcattgtgag
    22561 taattatgaa tacttcgggt tcttttttat gatttatgtt aatttcaatg gaat
//
LOCUS       DAMZLE010000031        20931 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMZLE010000031 DAMZLE010000000
VERSION     DAMZLE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20931
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.031"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            31..795
                     /locus_tag="POB92_001056"
     CDS             31..795
                     /locus_tag="POB92_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDI6578565.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(856..1650)
                     /gene="tagH"
                     /locus_tag="POB92_001057"
     CDS             complement(856..1650)
                     /gene="tagH"
                     /locus_tag="POB92_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDI6578566.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            1997..2809
                     /gene="tagG"
                     /locus_tag="POB92_001058"
     CDS             1997..2809
                     /gene="tagG"
                     /locus_tag="POB92_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDI6578567.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            2908..4011
                     /locus_tag="POB92_001059"
     CDS             2908..4011
                     /locus_tag="POB92_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6578568.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKTMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLNPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            4008..5069
                     /locus_tag="POB92_001060"
     CDS             4008..5069
                     /locus_tag="POB92_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6578569.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLPRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHTILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            5132..5530
                     /gene="tagD"
                     /locus_tag="POB92_001061"
     CDS             5132..5530
                     /gene="tagD"
                     /locus_tag="POB92_001061"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDI6578570.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(5647..6942)
                     /gene="pbp4"
                     /locus_tag="POB92_001062"
     CDS             complement(5647..6942)
                     /gene="pbp4"
                     /locus_tag="POB92_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDI6578571.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRSFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            7363..9090
                     /locus_tag="POB92_001063"
     CDS             7363..9090
                     /locus_tag="POB92_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578572.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYNIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            9447..10676
                     /locus_tag="POB92_001064"
     CDS             9447..10676
                     /locus_tag="POB92_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6578573.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GMIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            10931..11023
                     /locus_tag="POB92_001065"
     CDS             10931..11023
                     /locus_tag="POB92_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578574.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            11201..12034
                     /locus_tag="POB92_001066"
     CDS             11201..12034
                     /locus_tag="POB92_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI6578575.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            12316..13113
                     /locus_tag="POB92_001067"
     CDS             12316..13113
                     /locus_tag="POB92_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578576.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            13149..14153
                     /locus_tag="POB92_001068"
     CDS             13149..14153
                     /locus_tag="POB92_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6578577.1"
                     /translation="MTNRENSTPLKFLAYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNSLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            14150..15166
                     /locus_tag="POB92_001069"
     CDS             14150..15166
                     /locus_tag="POB92_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6578578.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGIATALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            15400..16368
                     /gene="dhaK"
                     /locus_tag="POB92_001070"
     CDS             15400..16368
                     /gene="dhaK"
                     /locus_tag="POB92_001070"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6578579.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            16410..16994
                     /gene="dhaL"
                     /locus_tag="POB92_001071"
     CDS             16410..16994
                     /gene="dhaL"
                     /locus_tag="POB92_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDI6578580.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            16987..17349
                     /locus_tag="POB92_001072"
     CDS             16987..17349
                     /locus_tag="POB92_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDI6578581.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            17465..17962
                     /locus_tag="POB92_001073"
     CDS             17465..17962
                     /locus_tag="POB92_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578582.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            18419..19486
                     /locus_tag="POB92_001074"
     CDS             18419..19486
                     /locus_tag="POB92_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578583.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            19637..20680
                     /locus_tag="POB92_001075"
     CDS             19637..20680
                     /locus_tag="POB92_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6578584.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
BASE COUNT         7141 a         2968 c         3828 g         6994 t
ORIGIN      
        1 aataatgaaa taagatagga gattcctgtt atgactgttg aagaaagatc caatacagcc
       61 aaagttgaca ttttaggagt cgattttgat aatacaacaa tgttgcaaat ggttgaaaat
      121 attaaaacct tttttgcaaa tcaatcaacg aataatcttt ttatagtaac agccaaccct
      181 gaaatagtga attacgcgac gacacatcaa gcgtatttag agttaataaa tcaagcgagc
      241 tatattgttg ctgatgggac aggagtagtc aaagcttcgc atcgtttaaa gcaacctcta
      301 gcgcatcgta tacctggtat tgagttgatg gatgaatgtt tgaaaattgc tcatgtaaat
      361 catcaaaaag tatttttgct aggggcaact aatgaagttg tagaagcggc acaatatgca
      421 ttgcaacaaa gatatccaaa catatcgttt gcacatcatc acggttatat tgatttagaa
      481 gatgagacag tagtgaaacg aattaaactg tttaaacctg attacatatt tgtaggtatg
      541 ggattcccta aacaagaaga atggattatg acacatgaaa accaatttga atctacagtg
      601 atgatgggcg taggtggttc tcttgaagta tttgctgggg ctaaaaagag agcgccttat
      661 atctttagaa aattaaacat tgaatggata tatagagcat taatagattg gaaacgtatt
      721 ggtagattaa agagtattcc aatatttatg tataaaatag ccaaagcaaa aagaaaaata
      781 aaaaaggcga aataatcatg atgacaaaaa taaaaccgag gaaatcctta aatggagatt
      841 ctcggttttt tcggtttatt taataacgaa gcgggactca tcgagtttgt ttctaaattc
      901 tttttgttcg gctttggatt tctttttaaa atcgttaagg aaagcttcat atttaggtaa
      961 tacatcatca agttcaccgt aatcttttaa ctttccgcct tcaatccaag caatcttagt
     1021 acaaaattgt ctcacttgtc ctaagttatg actaacgaaa aagatggttt tgttttgctc
     1081 tttaaactcg taaattttat ctaaacattt ttgtgcaaaa gtttggtcac ctacagataa
     1141 agcttcgtca atgactaaga tatctggatt aactgtgata ttaattgaaa aaccaagttt
     1201 tgcacgcata ccacttgaat actttttaac tggttgataa ataaactcac caagttcact
     1261 aaattcaata atcttaggtg tcatcgcttt aatttctttt cgcttaaagc ccatacataa
     1321 cattttaaat tcgatatttt caatccctgt aagttgtcca ctcaagccag cactaattgc
     1381 gataacgctg acttcaccat tacgatccac tttgccaaca gtaggcgaca aagaaccgcc
     1441 aatgatattg ctcaacgttg atttgccgga accattgatg ccaacaagcc ctatgacgtc
     1501 gccttcatat gcttttaaac taatgtcatc taaagcgaaa aatgttttgt ttttatgttt
     1561 gggaatgagc gcatctttca tacgttcttt atttgtacga taaatacgat attcttttgt
     1621 tacattttta atgtttaccg aaacgttcat ttgtagacct tccttattca catttatcta
     1681 gattataata tactactcaa cagttgttaa attttaaaac ctgttgtaaa gtgtatagaa
     1741 gattttgtta ttatcagagt tggtgttttg acacaaaatg ttaatcatca atgataacaa
     1801 tgatatttaa aaactaaact tatttcaact tacatgattg tatactataa tgtatttgta
     1861 ataaactaat attttaaaga actagacaat aattttgata gcatccatgt atagtgatag
     1921 tatttacaac aattattata atactattta gttaagtaga gaaatagtta aacatttgaa
     1981 agtgtggttt aatggaatgt cagcaatagg aacagttttt aaagaacatg taaagaactt
     2041 ttatttaatt caaagactgg ctcagtttca agttaaaatt atcaatcata gtaactattt
     2101 aggtgtggct tgggaattaa ttaaccctgt tatgcaaata atggtttact ggatggtttt
     2161 tggattagga ataagaagta atgcaccaat tcatggtgta ccttttgttt attggttatt
     2221 ggttggtatc agtatgtggt tcttcatcaa ccaaggtatt ttagaaggta ctaaagcaat
     2281 tacacaaaag tttaatcaag tatcgaaaat gaacttcccg ttatcgataa taccgacata
     2341 tattgtgaca agtagatttt atggacattt aggcttactt ttacttgtga taattgcatg
     2401 tatgtttact ggtatttatc catcaataca tatcattcaa ttattgatat atgtaccgtt
     2461 ttgttttttc ttaactgcct cggtgacgtt attaacatca acactcggtg tgttagttag
     2521 agatacacaa atgttaatgc aagcaatatt aagaatatta ttttactttt caccaatttt
     2581 gtggctacca aagaaccatg gtatcagtgg tttaattcat gaaatgatga aatataatcc
     2641 agtttacttt attgctgaat cataccgtgc agcaatttta tatcacgaat ggtatttcat
     2701 ggatcattgg aaattaatgt tatacaattt cggtattgtt gccattttct ttgcaattgg
     2761 tgcgtaccta cacatgaaat atagagatca atttgcagac ttcttgtaat atatttatat
     2821 gacgaaaccc cgctaaccat taataaatgg aagtggggtt catttttgtt tataatttaa
     2881 gtaaataaca tattaagttg gtgtattatg aacgttttaa taaagaaatt ttatcatttg
     2941 gtagttcgaa tactttctaa aatgattacg cctcaagtga ttgataaacc gcatatcgta
     3001 tttatgatga cttttccaga agatattaag cctatcatca aagcattaaa taattcgtcg
     3061 tatcagaaaa ctgttttaac aacaccaaaa caagcgcctt atttatctga acttagcgac
     3121 gatgttgatg tgatagaaat gactaatcga acattggtaa aacaaattaa ggctttgaaa
     3181 agcgcgcaga tgattattat cgataattat tacctattac taggtggata taataagact
     3241 tctaatcaac acattgttca aacgtggcat gcaagtggtg cattaaaaaa ctttggatta
     3301 acagatcatc aagtcgatgt gtctgacaag acaatggttc agcagtaccg taaagtttat
     3361 caagcgacgg atttttactt agtgggttgt gaacaaatgt cacaatgttt taaacagtct
     3421 ttaggtgcaa cagaagagca aatgttgtat tttgggcttc cgagaattaa taaatattac
     3481 acagctgata gagcaacggt taaggcagag ttaaaggata aatatggaat tacaaataag
     3541 ttggcattat atgtaccaac atatagagaa gataaagcag ataatagggc tattgataaa
     3601 gcttattttg aaaaatgttt accaggatat acactgatta ataaattaca tccatcaatt
     3661 gaacattcag acattgatga cgtatcttca atcgacacgt ctacattaat gctaatgtca
     3721 gatataatta ttagcgacta tagttcgctg ccaatagaag ctagcttgtt agatattcca
     3781 actatatttt atgtgtatga tgaaggaaca tatgataaag tgagaggcct gaatcaattt
     3841 tacaaagcaa taccggatag ctacaaagtg tatactgaag aagatttaat aatgacgata
     3901 caagaaaaag aacatctatt aaatccgtta tttaaagatt ggcataagta taatactgat
     3961 aaaagtttac atcagctcac agaatatata gataagatgg tgacaaaatg aggcttacga
     4021 taatcatacc tacatgtaat aatgaggcaa caattcgaca attgttaata tctattgaga
     4081 gtaaagaaca ctatagaatc ctttgtattg atggtggttc tactgatcaa acaattccta
     4141 tgattgaacg gttaccaaga gaactcaagc atatttcatt aatacaatta caaaatgctt
     4201 cgatagctac gtgtattaat aaaggtttga tggatatcaa aatgacagat ccacatgata
     4261 gtgacgcatt tatggtcata aatccaacat caatcgtatt gccaggtaaa ttagataggt
     4321 taactgcagc tttcaaaaat aatgataata ttgatatggt aatagggcag cgagcttaca
     4381 attaccatgg tgaatggaaa ttgaaaagtg ctgatgagtt tattaaagat aatcgaatcg
     4441 ttacattaac ggaacaacca gatttgttat caatgatgtc ttttgacgga aagttattca
     4501 gtgctaaatt tgctgaatta cagtgtgacg aaactttagc taacacatac aatcacacaa
     4561 tacttgtcaa ggcgatgcaa aaagctacgg atatacattt agtttcacag atgattgtcg
     4621 gagataacga tatagataca catgctacaa gtaacgatga agattttaat agatatatca
     4681 cagaaattat gaaaataaga caacgagtca tggaaatgtt actattacct gaacaaaggc
     4741 tattatatag tgatatggtt gatcgtattt tattcaataa ttcattaaaa tattatatga
     4801 acgaacaccc agcagtaacg cacacgacaa ttcaactcgt aaaagactat attatgtcta
     4861 tgcagcattc tgattatgta tcgcaaaaca tgtttgacat tataaataca gttgaattta
     4921 ttggtgagaa ttgggataga gaaatatacg aattgtggcg acaaacatta attcaagtgg
     4981 gcattaatag gccgacttat aaaaaattct tgatacaact taaagggaga aagtttgcac
     5041 atcgaacaaa atcaatgtta aaacgataac gtgtacattg atgaccataa actgcaatcc
     5101 tatgatgtga caatatgagg aggataactt aatgaaacgt gtaataacat atggcacata
     5161 tgacttactt cactatggtc atatcgaatt gcttcgtcgt gcaagagaga tgggcgatta
     5221 tttaatagta gcattatcaa cagatgaatt taatcaaatt aaacataaaa aatcttatta
     5281 tgattatgaa caacgaaaaa tgatgcttga atcaatacgc tatgtcgatt tagtcattcc
     5341 agaaaagggc tggggacaaa aagaagacga tgtcgaaaaa tttgatgtag atgtttttgt
     5401 tatgggacat gactgggaag gtgaattcga cttcttaaag gataaatgtg aagtcattta
     5461 tttaaaacgt acagaaggca tttcgacgac taaaatcaaa caagaattat atggtaaaga
     5521 tgctaaataa attatataga actatcgata ctaaacgata aattaatgta ggttattata
     5581 aaataaatat aaaacggaca agtttcgcag ctttataatg tgcaacttgt ccgtttttag
     5641 tatgttttat tttctttttc taaataaacg attgattatc atatgaacaa ttagtgctaa
     5701 tccagcgaca aggcatgcac caccaatgat agtgaataat ggatgttctt cccacatact
     5761 tttagcaaca gtatttgcct tttgaataat tggctgatga acttctacag ttggaggtcc
     5821 ataatcttta ttaataaatt ctcttggata gtccgcgtgt actttaccat cttcgactac
     5881 aagtttataa tcttttttac taaaatcact tggtaaaaca tcgtaaagat cgttttcaac
     5941 ataatatttc ttaccattta tcctttgctc acctttagac aatattttta catatttata
     6001 ctgatcaaat gagcgttcca ttaatgcatt ccccatcata ttacgttgct tctcgccacc
     6061 aaggttttta tagtctcccg cacccatgat aacttgatta attctaaatt tacctcgttt
     6121 ggtagtaatc gtatggttgt aatttgctgt atcacttgat ccagttttta aaccatctgt
     6181 acccggcaaa ctcatttttg caccttccaa tgaaaagttg aatgtgtaat acgtaactgc
     6241 atgcgttgtt ggtgctaact gctttgtaaa gtctaatatt ttaggtgtct ctttaatcac
     6301 gtgtaaatct aaaatggcat agtctctagc agtcgttaca gtacgttctt ggtctttata
     6361 ctttgttggt gcaaatgaac gtaatcttga attttcagca cccgttggat tgacgaaatg
     6421 tgtatttttc attccgatag ctttagcttt gttattcatt aaatcaacga aatcgctggt
     6481 gttttttgat accttcttag ctaaaattaa tgccgcggca ttactagaat tagatactgt
     6541 aatttgtaat aggtctgcga ttgtccatac ttgtccagga tatagtttcg tattactcaa
     6601 ctcaggtagt gtagacataa tatattcttt gttcgtcatt gtgacagtgt catcaagtga
     6661 aagctgcccc ttatttacag cttccaatgt taagtacatt gtcattaatt tggtcataga
     6721 cgctggattc cacttagtat cgatattgta ttgatacagt aattgtccag tttgacttac
     6781 attaacagca ctcgtcggtt cgtatgcagc tgacaaacct gcataaccat attgatttgc
     6841 tgcttgtaca ggggttacgt cactgttagc agcttgtgca tatggtgtca taatacttaa
     6901 tgttaaacat aaaatgatga taatagatat taaatttttc ataaagcgtt aatcttccct
     6961 tttccaattc ttaaatattc cctaaaagca atggttattc ctacttacgg aaatcattgc
     7021 taattcactt caccttaatt aaattgttga aaataaagtt ttctgcagtt aatttgaaaa
     7081 ataatgcaaa tatattacgt gtgtagctaa aggtgttata atgtttgtac gaagagcaaa
     7141 cttactcaaa agcgattaat tttcatgttt taatataaag actttgagaa gttattacaa
     7201 aaaatgcaat agaaatattc tatcatataa atgttatgag cggtattttg gggcaacact
     7261 ttatttgatt tttaaagttt tgttgggaga aagtatatga tagaaatgca tgtatctatc
     7321 taaatgaatt aactataaat ttcaaacaga agaggtaaaa ctatgaaacg agaaaatcca
     7381 ttgtttttct tatttaaaaa actatcatgg ccagtgggtc ttatcgttgc agctatcact
     7441 atttcatcac tagggagctt aagtggacta ttagtgccac tgtttactgg acgaattgta
     7501 gataaatttt ccgtgagcca tatcaattgg aatctaatcg cattatttgg tggtatcttt
     7561 gttatcaatg ctttattaag cggattaggt ttatatttat taagtaaaat tggtgaaaag
     7621 attatttatg cgatacgctc agttttatgg gagcatatca tacaattaaa aatgccattc
     7681 tttgacaaaa atgaaagtgg tcaattaatg agtcgattaa ctgacgatac gaaagtgata
     7741 aatgaattta tttcacaaaa gctacctaac ttattaccat caatcgttac attagttggg
     7801 tcactaatca tgttatttat tttagattgg aaaatgacat tattaacatt tataacgata
     7861 ccgatattcg ttttaattat gattcctcta ggtcgtatta tgcaaaagat atcgacaagt
     7921 acacaatctg aaattgcaaa cttcagtggt ttgttagggc gtgtcctaac tgaaatgcgt
     7981 cttgttaaaa tatcaaatac agagcgtctt gaattagata atgcacataa aaatttgaat
     8041 gaaatatata aattaggttt aaaacaggct aaaattgcgg cagttgtaca accaatttca
     8101 ggtatagtta tgttgctaac aattgcaatt attttaggtt ttggtgcatt agaaattgcg
     8161 actggtgcaa tcactgcagg tacattaatt gcaatgatat tttatgttat tcagttatct
     8221 atgcctttaa tcaatctttc aacgttagtt acagattata aaaaggcagt cggtgcaagt
     8281 agtagaatat acgaaatcat gcaagaacct attgaaccga cagaagctct tgaagattct
     8341 gaaaatgtat taattgatga cggtgtattg tcatttgaac atgtagactt taaatatgat
     8401 gtgaagaaaa tattagatga tgtgtcgttc caaatcccac aaggtcaagt gagtgctttt
     8461 gtaggtcctt ctgggtctgg taaaagtacg atatttaatc tgatagaacg tatgtatgaa
     8521 attgagtcag gtgatattaa atatggcctt gaaagtgtct ataatatccc gttatctaag
     8581 tggcgacgca aaattggata tgttatgcaa tcaaattcga tgatgagtgg tacaattaga
     8641 gacaatattt tatacggaat taatcgtcat gtttcagatg aagaacttat taattatgct
     8701 aaattagcga actgtcatga ttttatcatg caatttgatg aaggatatga cacgcttgta
     8761 ggtgaacgag gattgaaact gtctggcgga caacgtcaac gtattgatat tgctagaagt
     8821 tttgttaaaa atcctgatat tttgttactt gatgaagcaa cagctaatct cgatagtgaa
     8881 agtgaattga aaattcaaga agctttagaa acattgatgg aaggtagaac aacgattgtc
     8941 attgcgcatc gtttgtctac aattaaaaaa gccggtcaaa ttatattctt agacaaagga
     9001 caggtaacag gtaaaggtac gcattcagaa ctgatggcat cacatgcgaa gtataaaaac
     9061 tttgtagtgt ctcaaaaatt aacagattaa ttttatatat aagtaagctt ggagcaaata
     9121 cacatatacc atcgaggaaa ttaaagtgtg gcacattgat ggatatagat gttaataaat
     9181 tgcttcaagc ttttgtctat tttaaatcat ttgagaagtt acgacataat aattcttaaa
     9241 ttaatgaaat cgatatttta agaaaaaaat gctcatggta taatacaagt tataagcaaa
     9301 catacatata ttaaatactg tagccacgag tcataattct tcatatttta catagcaatt
     9361 taactgattt tagagtccat ggtacagaag tttgatattt caatgtttct aaatttttaa
     9421 aaaattaaat cataggtggg tgccaaatgt ttttattaat caacattatt ggtctaattg
     9481 tatttcttgg tattgcggta ttattttcaa gagatcgcaa aaatatccaa tggcaatcaa
     9541 ttgggatctt agttgtttta aacctgtttt tagcatggtt ctttatttat tttgattggg
     9601 gtcaaaaagc agtaagagga gcagccaatg gtatcgcttg ggtagttcag tcagcgcatg
     9661 ctggtacagg ttttgcattt gcaagtttga caaatgttaa aatgatggat atggctgttg
     9721 cagccttatt cccaatatta ttaatagtgc cattatttga tatcttaatg tactttaata
     9781 ttttaccgaa aattattgga ggtattggtt ggttactagc taaagtaaca agacaaccta
     9841 aattcgagtc attctttggg atagaaatga tgttcttagg aaatactgaa gcattagccg
     9901 tatcaagtga gcaactaaaa cgtatgaatg aaatgcgtgt attaacaatc gcaatgatgt
     9961 caatgagctc tgtatcggga gctattgtag gtgcgtatgt acaaatggta ccaggagaac
    10021 tggtactaac ggcaattcca ctaaatatcg ttaacgcgat tattgtgtca tgcttgttga
    10081 atccagtaag tgttgaagag aaagaagata ttatttacag tcttaaaaac aatgaagttg
    10141 aacgtcaacc attcttctca ttccttggag attctgtatt agcagcaggt aaattagtat
    10201 taatcatcat cgcatttgtt attagttttg tagcgttagc tgatctattt gatcgtttta
    10261 tcaatttgat tacaggaatg atagcaggat ggataggcat aaaaggtagt ttcggtttaa
    10321 accaaatttt aggtgtgttt atgtatccat ttgcgctatt actcggttta ccttatgatg
    10381 aagcgtggtt ggtagcacaa caaatggcta agaaaattgt tacaaatgaa tttgttgtta
    10441 tgggtgaaat ttctaaagat attgcatctt atacaccaca ccatcgtgcg gttattacaa
    10501 cattcttaat ttcatttgca aacttctcaa cgattggtat gattatcggt acattgaaag
    10561 gcattgttga taaaaagaca tcagactttg tatctaaata tgtgcctatg atgctattat
    10621 caggtatcct agtttcatta ttaacagcag ctttcgttgg tttatttgca tggtaatatg
    10681 ttgaagagtg actatgataa tacattttaa ctaataaata tgtccaggca tgtcgtctat
    10741 taatataggt gagatgcttg gactttttta ttattggtat aaagatattt aaatattttt
    10801 aaagttaccg aaattgaagc attataaaaa ccaagtgcac atggtaatac acttggcttt
    10861 tatgggaaat gaatattatt gtacatatga cagtaaggac taggtacagt catagtactt
    10921 cgagcaaaat ttgttttgtt attataaaca acacaaagga gataacttct ctattgaaga
    10981 agttaaaaac attatagcag acaatgaaat gaaagtaaat taaaaattca gaatattttt
    11041 aattattata ttgtgagtga tatttattag ggaaagctat tcttcatata aattagttaa
    11101 atagtaaatc tttgttagaa ctctttccat agagttgtga cagcattttt gttgtgctac
    11161 tatttttata gtctaataaa tatataaagg ggatggtttc gtgaataaaa cggttaaaga
    11221 tttaatacta gttgtcttag gttcatttat ctttgctgca ggtgtaaatg catttattat
    11281 ttctggtaac ttaggtgaag gcggggttac aggtttagca attattttat attatgcgtt
    11341 tcatatttca ccagccatca ctaacttctt ggtcaacgca gtattgattg ccataggtta
    11401 taaatttttg agtaagagaa gtatgtactt aactattctt gtaacaattc ttatttcaat
    11461 atttttgagt ttaacagaat catggcaagt agaaactgga aacagcattg tgaatgccat
    11521 ttttggtggt gtaagcgttg gactaggaat cggagtaatt atccttgcag gcggtacaac
    11581 agcaggtaca acaattttgg cgagaattgc aacgaaatac ctcgatgtaa gcacgccata
    11641 tgctttgctt ttcttcgata tgatcgttgt tgcaatttca cttacagtta ttccacttga
    11701 taaagtatta gtaacagtaa tatcacttta tataggaaca aaagtgatgg aatatgtcat
    11761 agaaggttta aacactaaaa aagctatgac gattatttca actaatcccg acaaacttgc
    11821 caaagcaata gacgagcaaa ttggaagagg tttaaccatt ttaaacggac atggctatta
    11881 tacgcgtgaa gaaaaagatg tcttatacgt tgttatttct aaaacacaag tttcaaaagc
    11941 aaagcgatta attaaacaaa tcgataaaga tgcattcctc gtaattcatg atgtaagaga
    12001 tgtctatggt aatggctttc ttgcagatga ataaataaat ggtatgagca cacatactta
    12061 aatagaagtc cacggacaag tttttgaact atgaagactt atctgtgggc gttttttatt
    12121 ttataaaagt aatatataag acatgacaaa tcgagctatc caatttaaaa agtaatgtta
    12181 gtcaataaga ttgaaaaatg ttataatgat gttcatgata atcattatca attgggatgc
    12241 ctttgaaaat tgataattta aaaatagaaa ttatttttta taaacagaaa gaattttatt
    12301 gaaagtaggg aaattatgaa tcgtttgcat ggacaacaag ttaaaattgg ttacggggat
    12361 aacacgatta taaataaatt agatgttgaa ataccagatg gcaaagtgac gtcaatcatt
    12421 ggtcctaacg gctgcgggaa atctactttg ctaaaggcat tgtcacgttt attggcagtt
    12481 aaagaaggcg aagtattttt agatggtgaa aatattcata cacaatctac gaaagagatt
    12541 gcaaaaaaaa tagccatttt acctcaatca cctgaagtag cagatggctt aactgttggg
    12601 gaattagttt catatggtcg ttttccacat caaaaaggat ttggtagatt aactgctgag
    12661 gataagaaag aaattgattg ggcaatggaa gttacaggaa ctgatacatt ccgacaccgt
    12721 tcaatcaatg atttaagtgg tggtcaaaga caacgtgttt ggattgcaat ggcattagca
    12781 caaagaactg atattatctt tttagacgaa ccaacaacat atttagatat ctgtcatcaa
    12841 ttagaaatac tagaattagt tcagaagcta aatcaggaac aaggttgtac aattgtcatg
    12901 gttcttcatg atatcaacca agcgattcgt ttctcagatc atcttattgc gatgaaagaa
    12961 ggggatatca tcgctacagg ttcaacagaa gacgtattaa cacaggaaat tttagaaaaa
    13021 gttttcaata ttgatgttgt tttaagtaaa gatcctaaaa ctggaaaacc tttattggta
    13081 acttatgact tatgtcgcag agcttattct taattaagta agttaatatg ataaaaagga
    13141 caattaacat gacaaataga gagaattcaa cgccattgaa gtttttagcc tatattatag
    13201 gtttaagtat gatactacta atcacactat ttatttctac attaataggt gacgccaaaa
    13261 ttcaagcctc tacaattata gaggctattt ttaattataa tcctagcaat caacagcaaa
    13321 acatcatcaa tgagattagg attcccagaa atatagcagc agtaattgtg ggtatggcgc
    13381 ttgcagtttc tggtgcgatt atacaaggtg ttacccgtaa tagtcttgct gatccggcgc
    13441 tcataggttt aaattcaggt gcttcatttg ctttagcatt aacatatgca gttttaccaa
    13501 acacttcatt tttaatattg atgtttgctg gatttttagg tgctattcta ggaggtgcta
    13561 ttgtattaat gataggccga tctagacgtg atggatttaa tccgatgcgt attattttag
    13621 cgggtgcagc agtaagtgct atgttaacag cgctaagtca aggtattgca ttagctttta
    13681 gactaaatca aacagtaaca ttttggactg ctggaggcgt ttcaggcaca acatggtcac
    13741 accttaagtg ggcaattcca ttaattggta ttgcgttatt cattatatta acaattagta
    13801 aacaacttac cattttaaat cttggtgaat cattagctaa aggtttaggt caaaatgtaa
    13861 caatgatcag aggcatatgt ttaattattg ctatgattct agcaggtatt gcagttgcta
    13921 tcgctggaca agttgcattt gtaggtttga tggtacctca tatagcaaga tttttaattg
    13981 gaactgatta tgctaaaatt ctaccattaa cagccttgtt aggtgggata ctcgtgcttg
    14041 ttgccgatgt gatagcacga tatttaggag aagcgcctgt tggtgcaatc atttcattta
    14101 tcggtgttcc ttacttttta tatttagtta aaaaaggagg acgctcaata tgattagttc
    14161 aaataataaa cgcagacaat ggattgcact ggctgttttt agcattctac tatttctagg
    14221 ttgtacttgg agtattacct caggtgaata caacatacct gttgaaagat ttttcaaaac
    14281 tttaattgga caaggtgatg ccattgatga gttaatctta ttagatttca gattacctcg
    14341 gatgatgatt actattttgg ctggcgcagc gcttagtatt agtggtgcaa tagtgcaaag
    14401 tgtcacgaaa aatccaatag ctgaacccgg tatattaggt attaacgcag gtggcggatt
    14461 tgcaatcgca ttatttattg caattggtaa aattaatgct gacaactttg tttatgtact
    14521 gccgttaata agtatactag gtggtatcgc cactgcattg attattttta ttttcagttt
    14581 taataaaaat gaaggtgtta cacctgcgag tatggtatta ataggtgtag gtttacaaac
    14641 agcattatat ggtggctcaa ttacaattat gtcaaaattt gatgataagc aatctgattt
    14701 catcgctgct tggtttgcag gtaatatttg gggtgacgaa tggccatttg tcattgcatt
    14761 tttaccgtgg gtgttgatta ttattcctta cttactattt aaatcgaata cactaaatat
    14821 tattcatacg ggtgataata ttgcacgagg tctaggtgta aggttaagca gagaacgttt
    14881 aatattattc tttatcgcag tgatgttatc atctgctgct gtagcagtag caggttcgat
    14941 ttcgtttatc ggattaatgg gtccgcatat tgccaaacgt atcgttggac cacgtcacca
    15001 gttgttttta ccaattgcca ttttagtagg ggcatgttta cttgttatag ctgatacaat
    15061 tggcaaaatt gtattacaac caggtggggt tccagcaggt attgtcgtag caattattgg
    15121 tgcaccgtat ttcttatatt taatgtacaa aacgaaaaat gtatagtgtc aaaggacaca
    15181 acttattgct atgaaaggca ctttattata aggcttttca tagcattttt tatttaatga
    15241 gccactcaag actatttatt ttttcaataa tgaaccatta agttatcaag aggatcttat
    15301 caaaaatata tttgataacg gtatcaggtt aattctttat gatagcgcat tcatttattc
    15361 tgttttatac tatgactgat aataccaagg aggtacaaca tgatgaaaaa gttaatcaat
    15421 aaaaaagaaa catttttaac tgatatgctt gaaggattgt taattgcgca cccagagtta
    15481 gatctgattg ctaatacagt tattgtaaaa aaagctaaga aagaacatgg tgtagcaata
    15541 gtctctggag gtggaagcgg acatgaacct gcgcatgccg gttttgttgc agaaggtatg
    15601 ctagatgcag cggtttgtgg cgaagtattt acatcaccta cacctgataa aatattagaa
    15661 gctattaaag cagtagatac tggtgatggt gtattactag ttgtaaaaaa ctatgcaggt
    15721 gacgtgatga atttcgaaat ggcacaagag cttgcagaaa tggaaggtat aaatgttcaa
    15781 actgttattg ttcgtgacga cattgctgtg acaaacgaag tacaacgtcg tggtgttgca
    15841 ggaacagtgt ttgttcataa gcttgccggt tatcttgctg aaaaaggtta ttcattaaca
    15901 gagataaaat cgcgtgtaga agcgttgtta cctgaaatta aaagtattgg tatggcaatt
    15961 gagccaccgc ttgttccaac tactggaaaa tatggctttg atattgaaga cgacaaaatg
    16021 gaaatcggta ttggtataca tggtgaaaaa ggtattcata gggaagaagt aaaggatatt
    16081 gatcatattg ttggaacatt gttagacgaa ttgtataaag aagttactgc caatgatgtc
    16141 atattaatgg taaatggtat gggtggtacg ccgttatctg aattaaatat cgtaactaaa
    16201 tatattcaac aaaatttagc tgcaagaacg gttaatgttg ctaaatggtt tgttggtgat
    16261 tatatgacat ctttagacat gcaaggtttt tctataacta tcgtgcctaa taaaccagaa
    16321 tatttggaag catttttagc accaacaaca agtcaatact ttaaataaga agtgaatatg
    16381 aataaatata catttatgag gtggcacaaa tgaaagtgaa tgatatgaaa gcacgtttat
    16441 taaatttaga agaaacgttt aaaaaacatg aatctgaatt aactgaatta gatcgagcaa
    16501 ttggtgatgg tgaccacggg gttaacatgg ttcgtgggtt tagtagtctt aaagacaaac
    16561 ttgatgatag ctcaatgcaa tcattgttca aatcgactgg tatggcattg atgtcaaatg
    16621 ttgggggtgc atcaggacca ctgtatggct ttagctttgt taaaatgtct gcagtcacca
    16681 aagatgatat ggataatcaa gatttcatta cactaattca ggcatttgcc gaagcggttg
    16741 aatcacgtgg taaagttact ttaaatgaaa agacaatgta tgatgtagta gcgcgagcag
    16801 cagagaagct tgaaaatggt gaaactttaa cattcaatga tttacagcaa ttagcagata
    16861 atacaaaaga tatggtagca acgaaaggta gagctgcata ttttggagaa gaatcaaaag
    16921 gttatattga tccaggtgct caaagtatgg tttatatttt aaacgctttg attggagatg
    16981 aagataatgc ctaaaattat acttgttagc cacagtaaag aaattgcaag tggtacaaaa
    17041 tctttgttaa agcaaatggc aggtgacgtt gatattatac caatcggggg attaccagat
    17101 ggttcaattg gaacttcatt tgatatcatc caagaagttt tgactaaatt agaggatgat
    17161 gcattgtgtt tttacgatat tggatcttca gaaatgaatg tagatatggc tattgaaatg
    17221 tatgatggta atcatcgtgt gttaaaagtt gatgcaccaa ttgttgaagg cagttttatc
    17281 gcagcagtaa agctatcaat cggcggttca attgatgatg cattagcaga aatcaaacaa
    17341 tcattttagt taaaatttac taataatgaa aaatgtaaac ctttttcaaa tgaaacttta
    17401 taaaaaatat gatagtatat atgtaaatgt ttaataaaat ctggagaaat aggaggacat
    17461 tgccatgcaa caccttataa aaaaacatgt attgaatggc gagtttgatt tagtacgaca
    17521 attgatgtcc gaaacagatt ttatggaatt tgaagaagca tatatttcaa gtgcgcatga
    17581 agtagaaagt atgatgtttt atacatgtat tttagatatg attaagtacg aagaatcatc
    17641 tgaaatgcat gacttagcat ttttattgct tgtgtatcca ctaagtgaat atgaaggtgc
    17701 tttggattct gcttattatc atgcagacgc ttccataaaa cttactgacg gcaaagaagt
    17761 taaaagtttg ttacaaatgt tattattgca tgcgatacca acacctgtta tttcagataa
    17821 gaaggctttt gatatcgcca agcaaatttt aaaattagat cctaataata atgttgctcg
    17881 taacgtctta aaagacactg ccaaacgtat ggacaacgtt gttgttgata taaatgaatt
    17941 acaccaacgt aatgcacgtt aattacattt caattatatt agcttaataa tagttttaac
    18001 atttggttgg gttgggacat atgttccagc cttttttaat acttaaaaac taacgaagta
    18061 tacttgtgtg cacaaatggt ttttatacaa cattttataa atttatacat tttaataaag
    18121 aacatacgat agatggttta aaccttgtta actgagaaat tttgatatgt attcttcgaa
    18181 atttaactaa atatacgaaa ttcaagaagc acaataatta atcatttttc ctatacaaaa
    18241 gttcgtatga ctgcattata aaagcataaa tttataattt ttttaaatgt cattgaacgt
    18301 gataatgtga atggattgag caattttgaa aaagtgaaaa ataacctatg cgacttgcaa
    18361 ttaattttca gtacgttata atgcacactg tgcaaaatta aggaggtcta ttattcacat
    18421 gatgatgaat aaagaagcaa caaaaattgg atttgcctac gtcggcattg tagtgggcgc
    18481 aggattttca actggacaag aagttatgca atttttcact aaatatggct tgtgggctta
    18541 tttaggtgtt attatatctg gttttatttt agcttttatt gggcgccaag tagcaaaaat
    18601 tggtactgcc tttgaagcga caaatcatga atcaacatta caatacgtat tcggtgaaaa
    18661 gtttagtaaa gtctttgatt atattttaat cttcttctta tttggtatag ctgtaaccat
    18721 gatagctggt gcaggcgcaa catttgaaga aagttataac atacctacat ggctaggtgc
    18781 tttaattatg acattagcga tttatattac gttgctatta gactttaata aaatagtacg
    18841 tgcactaggt atcgttacac catttttaat tgttttagtt gtattaatcg ctggcgttta
    18901 tttatttaaa ggtcatgttt cattagcaga agttaaccaa gtagtgcctg aagcaagtat
    18961 ttggaaggga atctggtttg gtacaatata tggtggatta gctttttctg taggttttag
    19021 taccatcgta gcaatcggtg gggatactga aaagcgtaca gtgtcaggtg caggcgcgat
    19081 gtatggtggt attatctata ctgtattact agcattgatc aactttgcat tgcaaagtga
    19141 atatccaact attaaaaatg cctcaattcc tacattgacg ttagcaaata atatccatcc
    19201 tttaatagca acagtgttat ctgttattat gctggcggtt atgtataata ctattctagg
    19261 actaatgtat tcatttgcag cacgttttac agaaccatac agtaaaaatt atcatatctt
    19321 tattattata atgatggtag caggttattt attaagtttc gtaggatttg ctgaattaat
    19381 taataagtta tatacaatta tgggatatgt aggcttattt attgtagtag ctgtaattat
    19441 taaatatttc aaacgtaaaa atgcggataa aaaacatatt gcttaatatc atatgaggga
    19501 tatccgaaac tttacaattg aatcactttg ttttaacctt aaaagcaatt cgtctctact
    19561 cttatcgggc gaattgcttt ttatatttat tcagtctatt aatatgagcg tctaacaaat
    19621 agagaggtac gatgtaatga ataaagataa taaatggacg atgataactg cgctttttat
    19681 aactgtaatc agtgtattgt tagcatttca tctgaaacaa cattatgacc aaattacaaa
    19741 tgagaaccat gctaataaag acaaaattaa tattaaaaat aaaaatgtgc gcatttatca
    19801 aaaccttaca tacaatagag ttttccctaa cagtaaatta gatattatta cacctgttga
    19861 tatgtcttct aatgccaaac tgccagttat tttttggatg cacggtggtg gttatattgc
    19921 gggtgataag cagtataaaa acccattatt agcgaaaatt gctgaacaag ggtacattgt
    19981 tgtgaatgta aattatgcat tggcgccaca atataaatat cccacaccat taattcaaat
    20041 gaatcaagca actcaattca ttaaagaaaa taaaatgaat ttacctattg attttaatca
    20101 agtaattatt ggcggtgatt ctgcaggtgc tcaattagct agccaattta cggcaataca
    20161 gacgaatgat cgcttaagag aagccatgaa atttgatcag tcattcaaac catcgcaaat
    20221 taaaggtgct atactatttg gtggttttta taatatgcaa acagttagag aaactgagtt
    20281 tccaagaata cagttattta tgaaaagtta tactggcgaa gaagattggg aaaagagttt
    20341 taaaaacatt tcacaaatgt cgacagtaaa acaatcgaca aaaaattatc caccaacatt
    20401 tttatctgtt ggagatagcg atccattcga aagtcaaaat atagaattca gtaagaaatt
    20461 acaagaattg aatgtaccag tagatacttt gttttatgat ggtacgcatc atttacatca
    20521 tcagtatcaa tttcacctta ataaacctga atcgatagat aatatcaaaa aagtgttact
    20581 tttcttaagt cgtaatacat cttctagtgg tattcaaact gaagagaaac cacaaataga
    20641 aaatccgagt aatgaattac cgttaaatcc tttaaactaa tgataaacag tagtaattta
    20701 ttacttaagc aacatttaag atcttcaaat tgaaaacgaa gaatttaaaa catgtggggc
    20761 taatgtgtaa gagtctgaga tataataaat tgttagcggt tctttatcat ttctatctca
    20821 ccctattatg atgtgacatt tattttacaa actaatttgt tttgaacctg aaaataactt
    20881 tttacaacta aattgtcgaa aaacagtgtg tacatgatat aataaattta t
//
LOCUS       DAMZLE010000032        20622 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMZLE010000032 DAMZLE010000000
VERSION     DAMZLE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.032"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..991
                     /locus_tag="POB92_001076"
     CDS             <1..991
                     /locus_tag="POB92_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578585.1"
                     /translation="GQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIG
                     TIIMFSNPNYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAA
                     LIIYLLLRSDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVI
                     VCGYFAFSIFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIH
                     VIILIILLKEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDH
                     PRQEVYTVESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            1285..3915
                     /gene="polA"
                     /locus_tag="POB92_001077"
     CDS             1285..3915
                     /gene="polA"
                     /locus_tag="POB92_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI6578586.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            3931..4803
                     /gene="mutM"
                     /locus_tag="POB92_001078"
     CDS             3931..4803
                     /gene="mutM"
                     /locus_tag="POB92_001078"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDI6578587.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            4819..5442
                     /locus_tag="POB92_001079"
     CDS             4819..5442
                     /locus_tag="POB92_001079"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI6578588.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            5612..6637
                     /gene="gap"
                     /locus_tag="POB92_001080"
     CDS             5612..6637
                     /gene="gap"
                     /locus_tag="POB92_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6578589.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            6848..7318
                     /gene="nrdR"
                     /locus_tag="POB92_001081"
     CDS             6848..7318
                     /gene="nrdR"
                     /locus_tag="POB92_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDI6578590.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            7319..8719
                     /locus_tag="POB92_001082"
     CDS             7319..8719
                     /locus_tag="POB92_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDI6578591.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKAMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYFGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            8719..9639
                     /gene="dnaI"
                     /locus_tag="POB92_001083"
     CDS             8719..9639
                     /gene="dnaI"
                     /locus_tag="POB92_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDI6578592.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNQRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            9732..9854
                     /locus_tag="POB92_001084"
     CDS             9732..9854
                     /locus_tag="POB92_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578593.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            10051..11988
                     /gene="thrS"
                     /locus_tag="POB92_001085"
     CDS             10051..11988
                     /gene="thrS"
                     /locus_tag="POB92_001085"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI6578594.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            12414..13907
                     /locus_tag="POB92_001086"
     CDS             12414..13907
                     /locus_tag="POB92_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6578595.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     misc_feature    13982..14107
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14136..14663
                     /gene="infC"
                     /locus_tag="POB92_001087"
     CDS             14136..14663
                     /gene="infC"
                     /locus_tag="POB92_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI6578596.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            14692..14892
                     /gene="rpmI"
                     /locus_tag="POB92_001088"
     CDS             14692..14892
                     /gene="rpmI"
                     /locus_tag="POB92_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI6578597.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            14939..15295
                     /gene="rplT"
                     /locus_tag="POB92_001089"
     CDS             14939..15295
                     /gene="rplT"
                     /locus_tag="POB92_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI6578598.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            15437..16045
                     /locus_tag="POB92_001090"
     CDS             15437..16045
                     /locus_tag="POB92_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI6578599.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNNFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            16064..16993
                     /locus_tag="POB92_001091"
     CDS             16064..16993
                     /locus_tag="POB92_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578600.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            16998..17108
                     /locus_tag="POB92_001092"
     CDS             16998..17108
                     /locus_tag="POB92_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578601.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            17156..18457
                     /locus_tag="POB92_001093"
     CDS             17156..18457
                     /locus_tag="POB92_001093"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI6578602.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            18608..19870
                     /gene="clpX"
                     /locus_tag="POB92_001094"
     CDS             18608..19870
                     /gene="clpX"
                     /locus_tag="POB92_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDI6578603.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDNVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            20024..20614
                     /locus_tag="POB92_001095"
     CDS             20024..20614
                     /locus_tag="POB92_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI6578604.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
BASE COUNT         7617 a         2777 c         3919 g         6309 t
ORIGIN      
        1 cggtcaatta tttatggaga atgtgacatt attccactgt atcattttaa ttttagcttt
       61 tctatatgaa tttactgcaa atcgaacact taattataaa ttattgtttt catttatgat
      121 tgctactata ggtacaatca ttatgttttc caatccaaat tatcgcaaaa ttttatttga
      181 aggatcagaa tatcaacaag tttcaaataa tcaaggtatc ttttcaaaat ttgcagaaat
      241 gatatcaacg tcacttccat atggtgtcat tttcagtcag attataattt taagtatgat
      301 tgcagcgctt attatttatc tactgcttag aagtgatcgg tatgtacatt taacaatatt
      361 aaagcgtcgt atcattatga taggatttat cactttaccg ctttattatt tactatttta
      421 caatcagttt ttattaaata aaaatacaga tattggattg gttggttttg tgaacgtaat
      481 tgtatgtggt tactttgcgt tttcgatatt tgtaggtatt tatttgtcta ttaatgatcg
      541 taaaacacaa atcactttat attccttact tattgcaata tgtgtttcgg cgtcgacact
      601 agtcattgtt actccaatag aaccaggtaa ttttttgatt gtttatacga tacatgtaat
      661 cattttaatt attttattaa aagaatttcg gaaatataag tcaatcaatg ttaattttat
      721 taaaggtgca tcactagttt tagctgtgat atatcttagt gcatttattt atgttcatta
      781 tgaacatgaa aaaagagttc agttattaaa acaacaaatt aaagatcatc cacgacaaga
      841 ggtttataca gtggaatcat tgccgtttga acattatatg catcatccgt caccaatcaa
      901 taaaaattac caagagtggt ttaataatta tgaagaatta ccgaaaatga ccaaagttaa
      961 atatattcca tttggttcag acgattcata acttattctt acctataaaa atttaaaata
     1021 gcagcataaa aatattaaaa agtagacata atcatgaaat gataatgtgc atctcaaaag
     1081 ttggactttt aacctgactt tatggagtgc tcatcaagaa caatcatgat tatgtctttt
     1141 ttatttttta gctaagtcta tataggcact tgttttattt gaaattaggt cgtgtataaa
     1201 tactctatga gatagtttct ttaattacga catcacagtc gaatatgcta aaatatatat
     1261 aaattagttc tggaggttaa aattgtgaat aaattagtat taatcgatgg taatagctta
     1321 agttttagag ctttttatgc attacctttg ttatcaaaca aagcaggcat tcataccaat
     1381 gcagtatatg gttttgcaat gttactagag aagattttaa aagaagaaaa gccaaatcat
     1441 tttttagtag cgtttgatgc aggtaaaacg actttcagac atgaaaaata tagtgaatat
     1501 aaaggtggac gccagaaaac gccgcctgaa ctaagtgagc aatttcctta tattcgccaa
     1561 ttattagatg cttatcatat taaacgttat gaattagata actatgaggc agatgatatt
     1621 atcggaactt taagtaaaga agcagataaa gcgggatttc agacaattat tattacggga
     1681 gaccgagatt taacacaact tgcaacggat aatgtaacca tttattacac taaaaaaggt
     1741 gttactgatg ttgatcatta tacacctgac tttattgcgg aaaaatacaa cgggttaaca
     1801 cctaatcaaa ttattgatat gaaaggatta atgggagata cttctgataa tataccaggt
     1861 gttgcaggtg ttggagagaa aacagcaata aaattgctaa accaatttga cacagtagaa
     1921 ggtgtctatg aacatttaga tgaaatttca ggtaaaaagt taaaagaaaa acttcaaaat
     1981 agcaaagaag atgcattaat gagtaaagaa ttagcaacga ttaatgtaga tagtccgatt
     2041 gaagtaaaac ttgaagatac gttaatgact catcaagatg aacaacaaga aaaaatcgaa
     2101 ttgtttaaga agttagaatt caaacaattg ttggctgaca ttgatcaatc agcaagcgtt
     2161 gaagatgcaa tagaaaagac atttgaaatt gaaacgtctt ttgataatat tgattttact
     2221 tcattgaaag aagcggccat ccatttcgaa ttagacggtg gtaactattt gcgaaataat
     2281 attttaaaat tctctttatt tacaggtgag aaacatattg taattaatgc ggatgacata
     2341 aataattatg tcgaactagt ttcatggtta gaaaatccga attcgaaaaa agtcgtatat
     2401 gatgctaaaa aaacatatgt agcatcacat agattgggga ttgatattca aaatatttct
     2461 ttcgatatta tgttggccag ttatataatt gatccatctc gtacgattag tgatgttcaa
     2521 tcagttgttt cattgtacgg tcaaagtttt gtgaaagacg atgtgagtat atatggaaaa
     2581 ggtaagaaat ttaaggtacc tgaagatgat gttttaaatc catatgttgc ttctattact
     2641 gatgcaattt actttgcgaa accaaatatg gataaacaat tagaagaata caatcaggta
     2701 gaactcttgg ctgatttaga gctaccgcta gctaagattt taagtgaaat ggaagaaatt
     2761 ggtatattta cagatgttca tgatttagaa gaaatggaaa aagaaattca agaaaaatta
     2821 gacgtcttga ttcgaaatat ccatgatgca gctggtgaag attttaatat aaattctcct
     2881 aagcaattag gtgttgtatt gtttgagaca ttacaattac ctgttattaa aaagacgaaa
     2941 acaggatatt ctactgctgt agatgtctta gagcaattgc aaggtgaaca tcctatcatt
     3001 gattatattt tagaatatcg tcaattatct aagttacagt ctacttatgt tgaaggattg
     3061 caaaaggtaa ttagtgatga tcaacgtatc catacacgtt ttaatcaaac tttagcgcaa
     3121 actggacgtt tatcaagtgt agatcctaat ttacaaaata ttccggttag acttgaagaa
     3181 gggcgtaaaa taagaaaagc ctttaaacca acttcaaaag atagcgttat attatcagca
     3241 gattattctc aaattgaatt gcgcgtatta gcacacatta cacaagatga gagtatgaaa
     3301 gaagcattta tcaacggcga tgatattcat acagcaactg ctatgaaagt atttggtgta
     3361 gaagctgatc aagtcgatag tttaatgcgt cgtcaagcaa aagcagttaa ctttggaatt
     3421 gtttatggga taagtgatta tggtttaagt caaagtttag gtattactcg taaaaaagca
     3481 aaagcattca ttgatgatta tttagctagt ttcccaggtg taaaacaata tatgtctgat
     3541 attgtaaaag atgccaaagc tttaggttac gtggaaacat tgctacatcg tcgacgctat
     3601 attcctgata ttacgagtcg taactttaat ttacgcggct ttgctgaacg tactgctatg
     3661 aatacgccaa tacagggcag tgctgcagat atcattaaac tggcaatggt taaatttgct
     3721 caaaaaatga aagagacaac atatcaagct aaactattat tacaagtaca cgatgaatta
     3781 atttttgaag tacctaagtc agaagtagat tcatttagtg aatttgtaga agagataatg
     3841 gaaaatgcat tgcaattaga tgttccatta aaagtagact caagttatgg tgcaacttgg
     3901 tatgatgcaa aataaagtga ggttaatggt atgcccgaat taccagaagt agaacatgta
     3961 aaaagaggaa ttgaacccta cgttataaat caaaaaattg aacacgttat attttcggat
     4021 aaagtaatcg aaggtaaagc tcaaggcaag gaaacaatta taaaaggaat tgaattagat
     4081 acttttaaaa ctttatcgga aggctatacc attacaaatg tagaacgaag aagtaaatat
     4141 atcgtttttc agctagataa taaacgagaa caacgtacat taattagtca tttaggtatg
     4201 gcgggcggtt ttttcattgt agatgaactt gaagatatta tgatacctaa ttatcgtaag
     4261 cattggcatg ttatttttga gttgtcaaat gacaaaaagt taatttattc agatatacgt
     4321 cgttttggag aaattagaaa tgttgcgtct gtagcatctt atccttcatt tttagaaata
     4381 gcgcctgaac ctttttcaaa tgaagcattg acgtattatt taaatcgaat tcatcaacag
     4441 tctaataaga ataaaccaat caaacaagtg attttggatc ataaagtaat cgcaggttgt
     4501 ggaaatattt acgcatgtga agcgttgttt cgtgcaggtg ttttaccaga taaaaaagta
     4561 aaagacttaa cacaccaaca acaagaaatg gttttttatt atgttagaga agtactagaa
     4621 gaaggtatta aatatggcgg tacaagtatt tcagattatc gtcatgctga tggaaaaact
     4681 ggtgagatgc aattgcatct taatgtatat aagcagccgg tatgcaaagt gtgtggaagt
     4741 caaattgaaa caaaaatcat cgcgacaaga aatagtcatt attgtccagt atgtcaaaaa
     4801 taaagaaaga gtgaaattat gccgaaagtt attggtctaa caggtggaat cgcctcagga
     4861 aaatcaacag tatcagaact cttatccgta ttcggtttta aagtagtaga tgctgataaa
     4921 gcagccaggg aagctgttaa aaaggggagt aaaggtttag ctcaagtacg agaagtcttt
     4981 ggtgatgaag caattgatga aaatggtgag atgaatcgtc gttatatggg tgatctagtg
     5041 tttaatcatc cagaaaaacg cttagaatta aatgctatca tacatcctat cgtgcgagat
     5101 attatggaag aagaaaagca agaatattta aaacaaggat ataatgtaat catggatatt
     5161 ccattattat ttgaaaatga attggaaaat acagtagacg aagtgtgggt tgtatacact
     5221 tctgaaagta tacaaatgga tcgtttaatg caacgtaata atttgtcatt agaagatgcg
     5281 aaagcacgtg tctatagcca aatttctatt gataaaaaaa gccgaatggc cgatcatgtt
     5341 atcgataatt taggggataa acttgaatta aaacaaaacc ttgagagatt gttagaagaa
     5401 gaaggttata ttgaaaagcc gaattacgga gaagaagatt aatattacac tataaataag
     5461 tcattacttt acgtacgcgt tgatgtatgt aagtaatgac tattttttat aaaaaagata
     5521 aataaatcaa cggaaaacgc tttcaaattt catataatat gctatactaa ttccataaag
     5581 tataacacat aaagatcaag gggtgctttt aatgtcaacg aatattgcaa ttaatggtat
     5641 gggtagaatt ggaagaatgg tattacgtat tgcattacaa aataaaaatt taaatgtagt
     5701 agcgataaat gctagttatc cacccgaaac aattgcacat ttaatcaatt acgatacgac
     5761 acatggaaaa tataatctaa aagttgaacc gattgaaaat ggattgcaag ttggagatca
     5821 taaaattaaa ttggttgctg atcgcaatcc tgaaaacttg ccatggaaag aattagatat
     5881 cgatattgct atagatgcaa ctggtaaatt taatcatggt gataaagcca tcgcacatat
     5941 taaagcaggt gccaaaaaag ttttgttaac tggtccttca aaaggtggac atgttcaaat
     6001 ggtagttaaa ggcgtaaatg ataaccaatt agatatagaa gcatttgaca tttttagtaa
     6061 tgcttcatgt actactaatt gcattggtcc agttgcaaaa gttttaaata atcagtttgg
     6121 aatagttaat ggtttaatga ctactgttca cgctattaca aatgaccaaa aaaatattga
     6181 taatccacat aaagatttaa gacgtgcacg ttcatgtaat gaaagcatta ttcctacttc
     6241 tactggtgcg gcgaaagctt taaaagaagt attaccagaa ttagaaggta aattacacgg
     6301 catggcatta cgtgtaccaa caaagaatgt atcgctcgtt gatttagttg ttgatttaga
     6361 aaaagaagta actgcagaag aagtaaacca agcttttgaa aatgcaggtt tagaaggtat
     6421 catagaagtc gaacatcaac cactagtgtc tgttgatttt aatactaatc caaattcagc
     6481 tattattgat gcaaaatcaa caatggtcat gtcagggaat aaagtaaaag ttattgcttg
     6541 gtatgataat gaatggggtt attcaaatag agttgtagat gttgctgagc aaattggagc
     6601 acttttgaca tcaaaagaaa ctgtaagtgc aagttaatac aattaattta tagcctaact
     6661 ctgacttcgg agttaggttt tttatttttt aatacaataa tattgtcatg caaattactt
     6721 aataagtaac gatataatgt tttttattgt ttcattattt ggttaaatga attaaattga
     6781 gggattcttt tttcatctct agatttatag tataattagg cataatcaaa ataaagaggg
     6841 tgatgtaatg aaatgcccga aatgtaattc tacacaatct aaagttgtag attcaaggca
     6901 tgccgatgaa ttaaatgcca ttcgaagacg aagagaatgt gaaaattgtg gaacacgttt
     6961 cactacattt gaacatatcg aagttagtca gcttatagtt gtgaaaaaag atggcacaag
     7021 agagcagttt tcaagagaaa agatacttaa tggacttgtg cgttcttgtg agaaacgacc
     7081 agttagatat caacaacttg aagacataac aaacaaggtt gaatggcaat tacgagatga
     7141 aggtcatacg gaagtgtctt cacgagatat aggtgaacac gttatgaact tgttaatgca
     7201 tgttgatcaa gtttcatatg ttagatttgc atcagtctat aaagaattta aagatgttga
     7261 tcaattgtta gcatcgatgc aagggatttt aagcgaaaac aaacggagtg atgcttaaat
     7321 gggacgacaa gccttcgaat tcggcttaag accaaaggat caattcaaag ttatgcaaca
     7381 ctttgattta aatacgaatc atttagaggt attgaatcgg ctgtatacac cgctaattgg
     7441 aacacaagca gtgggtttat accattttat gactcaattc gttaaagaaa gtcataatga
     7501 aacattgatt ctttctcatt atatttttat gaatgaactt aaaattaatt tactagagtt
     7561 tagacaacaa atggacttac ttgaagcgat tggattgtta aaggcgtttg tcaaacatga
     7621 tgaacaagaa acacaatttg tttaccaatt aattcaaccg ccttcagcac atttattttt
     7681 taatgatcca atgttgtcaa tctttttata tagtgaagtc gagcatcgtc gatttcatga
     7741 gttgaagaaa tactttgagt atcaacaaat tgatttgagt gaatttaaac aggtaacaag
     7801 acaatttact gatgttttta aagtgccgtc tacaaaaata gatatcgata caagtgatat
     7861 tccgattaat gaaccgtacc aaggtatcga tttatcaaat gaatcttttg actttgaaat
     7921 gttgagacaa atgttaggta aacattttat tagtcaagac attgttacca aagatgcaaa
     7981 gcgtttaata acacaactcg cgactttata tggtttaaca gcagatggca tgaagcatgt
     8041 tattttaaat tcaattacaa gtggacaaca gttatccttt gaagaaatgc gtaaacaagc
     8101 acgctcgtat tacttaatgg aacacgaaaa tcaaatgcct aaattacagg tgaaatctcc
     8161 tgctacatca tcttcgacgg gcaaatcttc agaagtgaat ccaaagccac aaagtgatga
     8221 atggtttgag ttacttgaac aaacgagtcc aattgatatg ttagcttctt ggtctgaatc
     8281 agaaccaact atatcgcaaa aggcaatggt tgaagaatta attgaacgtg aaaaaatgtc
     8341 ttttggcgtc attaatattt tgttgcaatt tgtcatgtta aaagaagata tgaaacttcc
     8401 taaagcgtat attttagaaa tagcatcaaa ttggaagaaa aaaggtatta agacagctaa
     8461 agaagcatat aattacgcta aaaaagttaa tcaacctaaa aatgagggtt ctagcggaaa
     8521 ttaccaaaag cgtggttcat actttgggca gcgtaatcgt atttcaaaag aaaaaacacc
     8581 taaatggtta gaaaatagag ataaacctag tgaagaagat tcggctaaag ataatagcgt
     8641 agatgatcaa caattagagc aagatcgaca agcatttcta gataaattat ctaaaaaatg
     8701 ggaggaggac agtcaataat gaagcaattt aaaagtataa ttaacacgtc gcaggacttt
     8761 gaaaaaagaa tagaaaagat aaaaaaagaa gtaatcaatg acccagatgt taagcaattt
     8821 ttggaagcgc atcgagctga attaacgaat gctatgattg atgaagactt aaatgtgtta
     8881 caagagtata aagatcaaca aaaacattat gacggtcata aatttgctga ttgtccaaat
     8941 ttcgtaaagg ggcatgtgcc tgagttatat gttgataata accgaattaa aatacgctat
     9001 ttacaatgcc catgtaaaat caagtacgac gaagaacgct ttgaagctga gctaattaca
     9061 tctcatcata tgcaacgaga tactttaaat gccaaattga aagatattta tatgaatcaa
     9121 cgagaccgtc ttgatgtagc tatggcagca gatgatattt gtacagcaat aactaatggg
     9181 gaacaagtga aaggccttta cctttatggt ccatttggga caggtaaatc ttttattcta
     9241 ggtgcaattg cgaatcagct caaatctaag aaggtacgtt cgacaattat ttatttaccg
     9301 gaatttatta gaacattaaa aggtggcttt aaagatggtt cttttgaaaa gaaattacat
     9361 cgcgtaagag aagcaaacat tttaatgctt gatgatattg gggctgaaga agtgactcca
     9421 tgggtgagag atgaggtaat tggacctttg ctacattatc gaatggttca tgaattacca
     9481 acattcttta gttctaattt tgactatagt gaattggaac atcatttagc gatgactcgt
     9541 gatggtgaag agaagactaa agcagcacgt attattgaac gtgtcaaatc tttgtcaaca
     9601 ccatattttt tatcaggaga aaatttcaga aacaattgaa ttttaaaatg attggtgtat
     9661 aatgaataca aatctaaatc gtttaaatga ttgaagacaa gatgatctaa tcaatattac
     9721 acagaaagcc attgtttgat gagaatatgg ttaataaatt agatgattac tacttcattt
     9781 atggtatttg taatgaatac ccggatcaag accgttatct taagcagaga tatttaatac
     9841 ataaattgta ttaaataact agggtggtac cacgacgatc tcgtcccttg tataggggcg
     9901 ggattttttg ttttttcaga cataaatgtt tgttggtgtc ataaattccc tgtttattgt
     9961 taataggttt aatgttaaaa cgatgattgt tgttcaattt tttaacgagg tcagataaaa
    10021 gtatttataa agcaaatagg agggtttaac atggaacaaa ttaatattca atttccagat
    10081 ggtaataaaa aggcgtttga taaaggtact actactgaag atatagcaca atcaattagt
    10141 cctggattac gtaaaaaagc tgttgccggc aaatttaacg ggcaacttgt agatttaaca
    10201 aaaccgcttg aaactgatgg atcaattgaa attgtgacac caggtagtga agaagcgtta
    10261 gaggtattac gtcattctac tgcacattta atggcacacg cgattaaaag gttatatggt
    10321 aatgttaaat ttggtgtagg tcctgtaata gaaggtggat tctactatga cttcgacatt
    10381 gaccaaaaca tctcatctga tgactttgaa caaattgaaa agacaatgaa acaaatcgtt
    10441 aacgaaaata tgaaaatcga acgaaaagtg gtttcacgag atgaagcgaa agagttattc
    10501 agcaatgatg aatacaaatt agaattaatc gacgcgattc ctgaagatga aaatgtaaca
    10561 ttatatagtc aaggtgattt tactgattta tgtcgtggag ttcacgttcc atcaacagct
    10621 aaaattaaag agtttaaact attatctaca gcaggtgcat actggcgtgg agatagtaac
    10681 aacaaaatgt tacaacgtat atacggtact gctttctttg ataaaaaaga attaaaagca
    10741 catttacaaa tgttagaaga gcgtaaagaa cgtgatcatc gtaaaattgg taaagagtta
    10801 gaattattca caaatagcca attagttggt gctggtttgc cattatggtt acctaacggt
    10861 gcaacaatta gacgtgaaat tgaacgttac attgttgata aagaggtgag catggggtat
    10921 gaccacgttt atacaccagt acttgctaat gttgatttat acaaaacatc tggtcactgg
    10981 gatcactatc aagaagatat gttcccacca atgcagttag atgaaactga atctatggta
    11041 ttacgtccaa tgaactgtcc acatcatatg atgatttatg cgaataaacc acattcatat
    11101 cgtgaattac ctatccgtat cgctgagcta ggaacgatgc atagatatga agcaagtggt
    11161 gctgtatcag gattacaacg tgttcgtggt atgactttaa atgattcaca tatctttgtt
    11221 cgacctgatc aaattaaaga agaattcaaa cgcgttgtaa acatgattat tgatgtgtat
    11281 aaagactttg gtttcgagga ttatagcttt agattaagtt atagagaccc tgaagataaa
    11341 gaaaagtact ttgatgatga tgatatgtgg aataaagctg aaaatatgct taaagaggca
    11401 gcggatgagc ttggcttatc gtacgaagaa gcgattggtg aagcggcatt ctatggtccg
    11461 aaactagatg ttcaagttaa aacagcgatg ggtaaagaag agacattatc aacagcacaa
    11521 cttgatttct tattaccaga acgttttgat ttaacttata ttggtcaaga tggtgaacat
    11581 catcgtccag ttgttattca tcgtggtgtt gtatcaacaa tggaacgatt tgtagcattt
    11641 ttaactgaag aaactaaagg tgcattccca acttggttag cgccaaaaca agttcaaatc
    11701 attccagtta acgttgattt acattatgat tatgcgcgcc aattacaaga tgaattgaaa
    11761 tctcaaggcg ttcgtgtaag tattgatgac cgtaatgaaa aaatgggtta taaaattaga
    11821 gaagctcaaa tgcaaaaaat accttatcaa atcgtagttg gggataagga agttgaaaat
    11881 aatcaagtga atgtgcgtca atatggatcg caagaccaag aaacagttga aaaagatgaa
    11941 tttatctgga atctagttga tgaaattcgt ttaaaaaaac atagatagac agttgtcgca
    12001 ataaaatgct ttgaaatttt tattgcgtat caagtttcac agggttgatt atgcgtgatg
    12061 aatcctgtat attacaagtt agttaaaata ttaaattgag ttagaggttg catgtttaat
    12121 tagtaacttg tcagaagtat ttatggtaca taagttgaac aagtgaaagg taaagatgcc
    12181 gaaatagata taaaccataa attatatcta ttgggacagt tttcgaatag gaactgtact
    12241 gtcacagaat gtgatgtgct accttatata gataattgcc aaagtggttg catatcttaa
    12301 aggtatgtag ccactttttt acttttaata tcactatgtt ctgtaaaaaa gggtatgaaa
    12361 gtgaataaag gttatttatt tcttggcctc taaaacatgg aaagggagct tatatgtcaa
    12421 aagttcaaaa tgaaagtaac aatgttgtca aaaggggact taaagatcgt catatttcta
    12481 tgattgcgat tgggggttgt attggtacag gtttatttgt aacttctggt ggagcaattc
    12541 atgatgcagg tgctttgggt gcattaatag gatacgcaat tatcggaata atggtatttt
    12601 tcttaatgac gtcacttggc gaaatggcta cgtatttgcc agtatcaggt tcatttagta
    12661 catatgctac aagatttgtt gatccatctt tagggtttgc gcttggttgg aactattggt
    12721 ttaactgggt agtaactgta gcagcagata ttacgattgc agcacaagtc attcaatatt
    12781 ggacaccatt gcaaggcata cccgcttggg catggagtgc gttgttctta gttataattt
    12841 ttagtctgaa ttcgttatca gttcgcgtct atggtgaaag tgaatactgg ttggcattga
    12901 taaaagtggt tacagttatt gttttcattg caattggttt attaacgatt gtcggaatca
    12961 tgggtggtca tgttgtagga ttcgaaatat ttaataaagg tgaaggtcct attcttggtg
    13021 gcaacttagg aggaagttta ttatcaattc taggtgtatt cttaatcgct ggtttctcat
    13081 tccaaggtac tgagttaatt ggtattacgg ctggtgaatc agaaaatcct gaacgtgctg
    13141 tgccgaaagc aattaaacaa gtattctgga gaattttatt attttacatt ttagccattt
    13201 ttgttatcgg tatgttaatt ccttatgata gtagtgcatt aatgggtggt agtgataatg
    13261 tagcaacgtc tccattcaca ttagtgttta aaaatgctgg atttgcgttt gcagcatcat
    13321 ttatgaatgc agttatttta acgtctgtgt tatcagcagg taactcaggt atgtatgctt
    13381 caactcgaat gctatattca atgagtaaag ataaattagc gtttgaagca tttggtaaaa
    13441 caaataaaaa cggtgtgcca tatatgtcgt tactcgttac agcaatcatt gtagtgataa
    13501 ttttcgtatt acaaagcctt acaaaaggtg catacgaata tatcgttgca gcaagtggtt
    13561 taactggttt tattgcttgg gtaggtatag cagtaagtca ttatcgattt agaagagcat
    13621 ttgataaaca aaattatgac aaatctaaat taaaatatac tgctaaatta ttcccattcg
    13681 gaccattatt tgctggcttt ttatgtattg tagtaattat tggtcaagat gttgacttta
    13741 ttaaaacagg agattttaat tttagtcgct tcttaattac gtatatggga atcccagtat
    13801 ttttagcatt ctttatttat cataagcttc gatataaaac gaaaaaaatt ccattagaac
    13861 aagtagattt acgtcaagac gtgtcaatgg atgaaataaa aggttaaaat aaaaataatt
    13921 acagttatag tgttgactta aatgtgaatt gttgatatga ttaacaacgt tgaatacgaa
    13981 acgaaccaag tagaagcacc cgcttctcac ctgtagcgac gcataaagca gttggcaggt
    14041 taatcacatt acacataact gtgtataagg aagagcgggt attttatacc tgctcttcct
    14101 ttttgcttgg tgcaaatttc gtaaaaattt tggaggtgtc aaccatagca aaagatcaaa
    14161 ctcaaatcaa tgacaaaatt cgtgcaaaag aattacgttt aatcggtcaa gatggtgaac
    14221 aaattggtgt taaatcaaag cgtgaagctt tagaaatggc tgaacgtgta gatttagact
    14281 tagtggtcgt tgcaccgaat gcgaaaccac cagttgcaag aattatggat tacggtaaat
    14341 tcaaattcga acaacagaaa aaagaaaaag aaatgaaaaa gaaacaaaaa attatcaatg
    14401 ttaaagaaat tcgtttaagt ccaacaattg aggaacatga tttccaaact aagttgaaaa
    14461 acggacgtaa attcttaact aaaggcgata aatgtaaagt atctattcgt ttcagagggc
    14521 gtgccattac gcataaggaa attggtcaac gtgtgctaga aaaatatgca gatgaatgca
    14581 aagatatagc aacagttgaa caaaaaccta aaatggacgg gcgtcaaatg tttatcatgt
    14641 tagcgccaac agctgaaaaa taattaaagc tattacagga ggacttatat tatgccaaaa
    14701 atgaaaactc accgcggagc agctaaacgt gttaaaagaa ctgcttcagg tcaattaaaa
    14761 cgttcaagag ctttcacatc tcacttattc gcaaacaaga gcactaaaca aaaacgtcaa
    14821 ttacgtaaag ctagattagt gtctaagagc gatatgaaac gtgtaaaaca attattagca
    14881 tacaaaaaat aagaacaaat acagaaaacg gtaggaatta cctaaggagg aattttttat
    14941 gccacgagtt aaaggtggaa cagtaacaag agcgcgtcgt aaaaaaacga ttaaattagc
    15001 taaaggttac ttcggttcaa aacatacatt atacaaagta gctaagcaac aagtaatgaa
    15061 atcaggtcaa tatgctttcc gtgaccgtcg tcaacgtaaa cgtgacttcc gtaaattatg
    15121 gattacacgt atcaacgcag cagctcgtca acatgaaatg agctactcac gtttaatgaa
    15181 cggtttgaaa aaagctggta tcgacattaa ccgtaaaatg ttatcagaaa tcgcaatttc
    15241 tgacgaaaaa gcatttgctc aattagtaac taaagctaaa gatgctttaa aataataaca
    15301 cttataaaaa agagatggca ttgtcatctc ttttttattt tttaaaaagc aaaatgccac
    15361 atttaatcgc taaaatatga catatttgct aaaatgcatt taagagaaca aacattaaag
    15421 tgaggagttt tatacgatgt ctaaatttga tgaacaaatc attgtagtac ctagagaaat
    15481 tatttttaat aatgaaaaga ataatttcaa tgggttttta aataagaata aaccagaagg
    15541 tcaaaatatt tttgatgcgc taagtcaata tgaagttaag cgacgcggcg atatggaaga
    15601 ggatccatca tataaacaac tcatttctta ttgtttactt gaaaatgagc atggcgagat
    15661 attagtgtat gaacgattat ctggcggtgg agaagctcga ttgcatggac aatcttcaat
    15721 aggtgtaggc ggtcatatga atgatgttcc aggagcagaa tctattaacg aagtattgag
    15781 agttaatgca cagagagaat tagaagaaga agtaggttta agtgagcaag attcacaaaa
    15841 tatggaatat atcggtttta ttaatgacga taataatgaa gtgggcaagg tacatattgg
    15901 tgttgtattt aaaatcactg taagtacgaa tgatgtagaa gctaaagaaa cagatacttt
    15961 acgaataaaa tgggttgaaa aaggcaacat agagtcatat gatgatttcg aaacgtggag
    16021 cgcattaatc cttcaagatt tataatcaaa cgaggtgaca tatatgtcag atattattca
    16081 gtttcctaac tcttcgaaaa agttatataa agatataaaa cgtgctgaac aagaccaaaa
    16141 ctatgacctg atgtatgaat atattgtgca atatgaacgt caatttgaac tcactgaaga
    16201 aatagcaatg atgaaatgcc gtatgttata tgagacgggg tcatttcttg aattaagaga
    16261 agaaacaatt gtcttattga aaactggcat acaacaatat gatgcattga tgatttatta
    16321 cgtgaaaagt ttgattggtt tgggacaata ttttgaagcg gtagaagtaa ttcatcaaat
    16381 tattgatgaa gtcaaagatc acaaaacaag aatggcatta catcctctta aagaatttgc
    16441 taagtcaaaa ttaattgaag atgaaaaacg attgactcag tcattaactg attttgatac
    16501 gttatcaatg agggaacaga cgcacttgat tttgaagtta atagacaatg gtcattttca
    16561 atttcaagaa acggtattat atatattaaa atctaatacg tacagttata acctcattag
    16621 tttaatgatt gagtatttaa ggtttgcaaa ttgtacacaa gaactgacaa ttgaaaagta
    16681 tggtatggat gtaacttttg taccagctaa tttaaatggg ctagaacata caacactgaa
    16741 agaaaaagtt atacctaacg ttatggaaac attaaatgat ggtgcattac atatcgctga
    16801 agaagcacac catattatga ataatcactc tatcatgatt tatccaattg atattgaaac
    16861 tttgtttgaa acgaataaat ggattaatgc atacgaatgt tatttcaaaa atatgttagg
    16921 acttaaatgt gaattacaaa gtatcgatgc atttaatttt attcagcaat tagatttgaa
    16981 taataatagt taaattgttg caaaataata ctcagcattt taatatagtt ttgacgaaaa
    17041 aagttgattt acaaatatat aaacgttgtg atttcaatgt ttgtataggg aatagaccta
    17101 tgatataata gggaagttga aaaaataaat tagttaatca tggaggtata tatacatgac
    17161 agcaacttgg gaaaaaaagg aaggtaacga aggtttatta actgttactg ttcctgcaga
    17221 aaaagtaaac aaagcgttag atcaagcatt caaaaaagtg gttaaacaaa ttaacgtacc
    17281 tggattccgt aaaggtaaag tgccacgtcc aatttttgaa caacgctttg gtgtagaagc
    17341 attatatcaa gatgctatcg acattttatt accagatgct tatggtgaag caattgacga
    17401 aactgatatt aaaccagttg cacaaccaga agtaagtgtt actcaaattg aaaaaggtaa
    17461 agatttcatt tttgaagcaa cagttacagt tgagccagaa gttaaattag gagactataa
    17521 aggtcttgaa attgaaaaac aagaaactga attatctgat gatgagttac aagaagcgat
    17581 tgaccacagc ttaggacatt tagctgaaat ggtagttaaa gaagatggtg ttgttgaaaa
    17641 tggcgacaca gttaacattg actttagtgg ttcagttgac ggagaagaat tcgaaggtgg
    17701 acaagctgaa ggttacgatt tagaaatcgg ttcaggttca ttcatacctg gtttcgaaga
    17761 gcaattagaa ggtatgaaag ttgacgaaga aaaagatgtt gtcgtaacat tcccagaaga
    17821 ataccatgct gaagaattag ctggtaaaga agcaactttc aaaacaaaag ttaacgaaat
    17881 taaatttaaa gaagttccag aattaacaga tgaaattgct aatgaattag atgcagaagc
    17941 aaatacagta gacgagtaca aagaaaactt acgtaaacgt ttagctgaac aaaaagctac
    18001 agatgctgaa aatgttgaaa aagaagaagc gattacaaaa gctactgata atacaacaat
    18061 cgatattcct gaagcaatgg ttaatactga attagatcgt atggtgtctg aatttgcaca
    18121 aagaattcaa caacaaggtt tagatttaca aacgtacttc caaatctcag gtcaagatga
    18181 aactcaatta agagagcaaa tgaaagacga tgcagaacaa cgtgttaaaa ctaacttaac
    18241 tttaactgcg atcgctgaag ctgaaaaaat cgaagctact gatgaagata tcgataaaga
    18301 attagaaaaa atgagtaaac aatttaatat ctcagttgaa gatatcaaaa atactttagg
    18361 taatactgat atcattaaaa atgatgttcg tatccaaaaa gttatcgatt tattaagaga
    18421 taacgcaaag ttcgttgaag gaactaaaga agattaatcc tcattaaata ttaaattaca
    18481 aaaatgagta gcagatgcat agcttatgta tctgctacta ttctttaagc aaaaagtttg
    18541 tatgttaata tgttgcattg taatatccaa tctagtatag tctttaacga ataggggtgt
    18601 aaaaagaatg tttaaattca atgaagatga agaaaatttg aaatgctctt tctgcggaaa
    18661 agaccaagat caagtaaaaa aacttgtagc aggaagtggt gtatatattt gtaatgagtg
    18721 tattgaatta tgctcagaaa tcgtcgaaga agaattagct caaaacactt ctgaagcgat
    18781 gacagaatta cctactccta aagaaattat ggatcattta aacgaatatg ttattggtca
    18841 agaaaaagct aaaaaatctt tagctgtagc tgtttataac cactataagc gtattcaaca
    18901 attaggacca aaagaagatg atgttgaatt acaaaaaagt aacattgcat taattgggcc
    18961 aacaggtagt ggtaaaacat tattagctca aacattagcc aagacgttga atgtaccatt
    19021 tgcaattgca gatgcgacaa gtttaactga agctggttat gtaggcgatg atgttgaaaa
    19081 tatcttgttg agattaattc aagcagctga ctttgacatt gataaagccg aaaaaggtat
    19141 tatttatgta gatgaaattg ataaaattgc acgtaaatct gaaaacacat ctataacacg
    19201 tgacgtttca ggtgaaggtg ttcaacaagc attgcttaaa atcttagaag gtacgactgc
    19261 aagtgttccg ccacaaggtg gacgcaaaca tccaaaccaa gaaatgattc aaattgatac
    19321 aacaaatatc ttatttattc ttggtggtgc ctttgatggt attgaagaag tgattaagcg
    19381 ccgtcttggt gaaaaagtta ttggtttctc aagcaatgaa gctgataaat atgacgaaca
    19441 agcattatta gcacaaattc gcccagaaga tttgcaagcc tatggtttga ttcctgaatt
    19501 tatcggacgt gtgccaattg tagctaattt agaaacatta gatgtaactg cgttgaaaaa
    19561 catcttaacg caacctaaaa atgcacttgt gaaacaatat actaaaatgc tggaattaga
    19621 taatgtggat ttagagttca ctgaagaagc tttatcagca attagtgaaa aagcaattga
    19681 aagaaaaaca ggtgcgcgtg gtttacgttc aatcatagaa gaatcgttaa tcgatattat
    19741 gtttgatgtg ccttctaacg aaaatgtaac gaaggtagtt attacagcac aaacaattaa
    19801 tgaagaaact gaaccagaac tatacgacgc agaaggcaat ttaattaata atagtaaaac
    19861 atcagcttaa tcattgatgt gttataaaag tgaaaagctc caatcaaagt tgacgctcaa
    19921 atagtttaac tttgatcgga gcttttttat aatgtatacc gaaaggttca aatattatat
    19981 aatggtagag ttgaaagaga atataaaaaa gtgaggatat aaaatgaaag ttaatcctaa
    20041 taatatagaa ttaatcatta gtgcagtaaa agaagaacaa tatccagaaa cagaattgtc
    20101 tgaagttgca ctgagcggtc gatctaatgt aggtaagtct acatttatca atagtatgat
    20161 tggcagaaaa aatatggcac gtacatcaca gcaacccggc aaaacgcaaa cgttaaattt
    20221 ttataatata gatgaacaac ttatttttgt ggatgttcca gggtatggat atgctaaagt
    20281 aagtaaaaca caacgtgaaa aatttgggaa aatgattgag gaatatataa ctaagagaga
    20341 gaatttgcaa ttagttattc aattagttga tttaagacat gatccaacac aagatgatat
    20401 cttaatgtac aattatttga aacattttga tattcctact ttagttatat gcactaaaga
    20461 agacaaaatt ccaaaaggta aagttcaaaa gcatattaaa aatattaaga cacaattaga
    20521 tatggaccca gacgatacaa ttgtaagtta ttcatcaatt caaaataata aacaacaaca
    20581 aatatggaat ttaattgaac cgtatatttc atagtttttg ta
//
LOCUS       DAMZLE010000033        20346 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMZLE010000033 DAMZLE010000000
VERSION     DAMZLE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.033"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..409
                     /locus_tag="POB92_001096"
     CDS             <1..409
                     /locus_tag="POB92_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578605.1"
                     /translation="TMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVTD
                     FSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINIS
                     FIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            689..1129
                     /locus_tag="POB92_001097"
     CDS             689..1129
                     /locus_tag="POB92_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDI6578606.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1472..1945
                     /locus_tag="POB92_001098"
     CDS             1472..1945
                     /locus_tag="POB92_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6578607.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1920..2606
                     /locus_tag="POB92_001099"
     CDS             1920..2606
                     /locus_tag="POB92_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6578608.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2606..3661
                     /gene="saeS"
                     /locus_tag="POB92_001100"
     CDS             2606..3661
                     /gene="saeS"
                     /locus_tag="POB92_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDI6578609.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3733..4716)
                     /gene="csbB"
                     /locus_tag="POB92_001101"
     CDS             complement(3733..4716)
                     /gene="csbB"
                     /locus_tag="POB92_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDI6578610.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(4848..5687)
                     /locus_tag="POB92_001102"
     CDS             complement(4848..5687)
                     /locus_tag="POB92_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6578611.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5909..7258)
                     /locus_tag="POB92_001103"
     CDS             complement(5909..7258)
                     /locus_tag="POB92_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6578612.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(7479..8660)
                     /gene="nagA"
                     /locus_tag="POB92_001104"
     CDS             complement(7479..8660)
                     /gene="nagA"
                     /locus_tag="POB92_001104"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6578613.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(8968..10926)
                     /locus_tag="POB92_001105"
     CDS             complement(8968..10926)
                     /locus_tag="POB92_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6578614.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GVDSFKPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFAAGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10932..11852)
                     /gene="pfkB"
                     /locus_tag="POB92_001106"
     CDS             complement(10932..11852)
                     /gene="pfkB"
                     /locus_tag="POB92_001106"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI6578615.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11849..12613)
                     /locus_tag="POB92_001107"
     CDS             complement(11849..12613)
                     /locus_tag="POB92_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6578616.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12863..13345)
                     /gene="ybaK"
                     /locus_tag="POB92_001108"
     CDS             complement(12863..13345)
                     /gene="ybaK"
                     /locus_tag="POB92_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDI6578617.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13518..13970)
                     /locus_tag="POB92_001109"
     CDS             complement(13518..13970)
                     /locus_tag="POB92_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578618.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENMKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(14263..15429)
                     /gene="norA"
                     /locus_tag="POB92_001110"
     CDS             complement(14263..15429)
                     /gene="norA"
                     /locus_tag="POB92_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDI6578619.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            15638..16060
                     /locus_tag="POB92_001111"
     CDS             15638..16060
                     /locus_tag="POB92_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6578620.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            16246..16863
                     /locus_tag="POB92_001112"
     CDS             16246..16863
                     /locus_tag="POB92_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDI6578621.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            16860..17144
                     /locus_tag="POB92_001113"
     CDS             16860..17144
                     /locus_tag="POB92_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578622.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(17299..18672)
                     /locus_tag="POB92_001114"
     CDS             complement(17299..18672)
                     /locus_tag="POB92_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI6578623.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(18758..20311)
                     /locus_tag="POB92_001115"
     CDS             complement(18758..20311)
                     /locus_tag="POB92_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDI6578624.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVTATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVDNKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         6724 a         3739 c         2845 g         7038 t
ORIGIN      
        1 aacgatgttt atcatttgga cgatactaca gttttacatt gataaaaaat taaaacctgt
       61 atacattaca gaccaaaaat ttattgctat catattaacg attgttgtca gtttaacgca
      121 acgtgttacc gacttttcaa gtacacaatc aatatatatg tctattccat ttttagcccc
      181 tgcaattttc atcattggtg gtattatgtt gtttatttca acatttaata gcttagatga
      241 aactgctgaa aataataaca aaataaagaa actaatgatt aaaggactta tcattattaa
      301 catttcattt atcgttatga tggttttaac accatattgg tacttgtatt taatcgtcta
      361 tcttattttc ttgttgtttt tattgtggca aaaggtttat aaattttaat accaaaacta
      421 taaaacactt ctgatattct tagttcaaaa tatcagaagt gttttatagt gttatctagt
      481 tcagataaat attttctcac ctaaaaaaat gccctcctct tattttgact cctatttatt
      541 taaatcagac aattattttc attttcaaat tattctttct tcaatattag ttaagcgata
      601 tttaaacgaa gttaagaatt agttaatggc atattatttg ccttcatttt aaacttaact
      661 tatcaaattg aagaaatgag gagttagcat gaatacaaaa tattttttag cagttggtgc
      721 tgttgcctct gtattaactt taggcgcttg tggtaattct aattcacaag atcaaggtaa
      781 caaaactgaa caaaaaacaa agtcagaaga tagcaatgtt aaaactgata aaacaaaaca
      841 cctaacaggt acattcagtt ctaaaaacgg tgaaactgtt gaaggtaaag ctgagattaa
      901 aaatggtaaa ttaatgctta ctaactacaa atcatcaaaa ggtccagatt tatacgtcta
      961 cctaacaaaa aatggcgaca ttaaaaacgg taaagaaatc gcaatggttg actacgataa
     1021 agaaaaacaa acatttgatc ttaaaaatgt agatttaagc aaatatgatg aagtaacaat
     1081 ctattgtaaa aaagctcacg tcatcttcgg cggcgctaaa ttaaaataat caatttctga
     1141 gttaaacttt tatttacaac atactattac tatacattac aaattttaaa aatatacatt
     1201 acactcatta ctcaatggaa agcgtatgat ttcacagccc ccctagcttg tagaaatcat
     1261 actttccttt tttcaatata tatacaacta ttaaatccca taagattgta gagcacataa
     1321 gtaaaatttt tttagagctt gaggtttgtt tagcttaagc aacccatgag ctcaaacact
     1381 tcctgttcac ataacactac aaatcgcatt atgttgctta atcttatgtt tatataaatt
     1441 acacacaata aatagaaaga atgtgaacat catgaataaa ttattgctac tcgttacatt
     1501 tatcattcgt gtgggttcag gtattgttat gttaatgcaa ggctacgaaa aattaacggg
     1561 cggatttacg ctgaaaggtt tagtaccagt catcgctaac aatactgatt caccagagtg
     1621 gtataagtgg tttttcgcaa atatagttgc acatacgacg tcattatttg atattgttgt
     1681 cccactcgga gagattgcaa ttggattagg tttaattttt ggagtttttg catatgctgc
     1741 tagtttcttt ggagcctttg ttatgataaa ttatatctta gcagatatga tatttacgta
     1801 tcctcttcaa ttaactttct ttatcctttt actaatgagt cactcattgt taaaacagat
     1861 ttcacttaaa gaaatcatta attactttag aggtcgtaag aacagaggtg aaaaaataga
     1921 tgacccactt actgatcgtg gatgatgaac aagacattgt agacatttgt caaacctatt
     1981 ttgaatatga aggttacaaa gtaacaacga caactagcgg taaagaagca atttctttac
     2041 tatcaaatga tattgatatc atggtacttg atatcatgat gccagaagtt aatggttacg
     2101 acattgtcaa agaaatgaaa aggcaaaaat tagatatccc ctttatctat ttaactgcca
     2161 aaacacaaga acatgatacc atttacgcct taactttagg tgcagatgac tatgtcaaaa
     2221 aaccatttag tccaagggaa ctcgttttac gtattaataa tttacttaca agaatgaaga
     2281 aataccatca tcaaccagtt gaacaactgt cgtttgatga attaacactt attaacttaa
     2341 gtaaagttgt gactgtaaat ggtcacgaag tccctatgcg tattaaggaa tttgagttat
     2401 tgtggtattt agcttctaga gaaaatgaag ttatttctaa atcagaatta cttgaaaaag
     2461 tttggggata tgactattac gaagatgcta ataccgtgaa tgtccatata caccgtatta
     2521 gagaaaaatt agaaaaagag agctttacaa catataccat cacaactgta tggggattag
     2581 gatataaatt tgaaaggagc cgataatggt gttatcaatt agaagtcaaa tcattattgg
     2641 cgtcgtatcg agtatactat taacttcaac tattttagca attgcatata ttttaatgtg
     2701 gtttaacggc catatgacac taactttgac cttaacgaca ataattacaa gctgtttaac
     2761 cttattaata tgtagtattt ttattaatcc acttatacaa aaaattaagc agtttaatat
     2821 aaaaactaag caatttgcta acggaaatta cgcaagcaat gataaaacgt ttaattcacc
     2881 aaaagaaatt tatgaattaa atcaatcttt taataaaatg gcttctgaaa ttacgcaaca
     2941 aatgaatcaa attaaatccg aacaacaaga aaaaacagaa ctgattcaaa acttagccca
     3001 tgatttaaaa acacctttag caagcattat ttcatattct gaaggactac gtgatggtat
     3061 aatcacaaag gatcatgaga ttaaagagtc atacgacata ttaattaaac aagcaaacag
     3121 attatcaaca ttatttgatg atatgactca tattatcact ttaaatacag gtaaaacata
     3181 tcccccagaa ttaatacaac tagaccaatt acttgtatca atattgcaac catatgagca
     3241 acgtatcaaa catgaaaacc gcacattaga agtgaatttc tgtagcgaaa ttgatgcatt
     3301 ttatcaatat cgaacgccac ttgagcgtat tttaacaaac ttacttgata atgcgctaaa
     3361 attttcaaat gttggtagtc gcattgatat taatattagt gaaaacaaag atcaagatac
     3421 tatcgacatt gctattagcg atgaaggtat tggcattata ccagaactac aagaacgtat
     3481 attcgaacgt acattcagag tagaaaactc tcgtaataca aaaacgggtg gttctggatt
     3541 aggcttatat atagctaatg aactcgcgca acaaaataac gcaaaaatca gtgtaagcag
     3601 tgatatagat gtaggaacta cgatgactgt aacattacac aaattagaca ttacgtcata
     3661 atccgattta tttataaaat aaaatgcaaa gactaaaaag aagctcccat taatgagggc
     3721 ttcttttttt gtttatttag aataaacttt atgggtatcc ttctcatcat tttcaatact
     3781 tgaaagattt gtagcttgaa taatatattt aggacgtgcc ttaacttcat aatatatcct
     3841 gccaatatat tcacctacaa caccaattga aattaactgt atgccgccta ataataaaat
     3901 agctgcaatc gttgaaaaat atcccggaat attaacacca gatatcataa tattgatgaa
     3961 taaatagata atatatagca cgcttattga aaagataaac aagccaagat aaatcatcat
     4021 tctcaaaggt ttactattaa aggaaatcaa tccatcaata ccataattaa ataacttttt
     4081 aaaggaccac ttagattccc ctttttgtct ctcaacattt tgatacgtaa acactttagt
     4141 attatagcct atccattcaa ataacccttt tgaaaatcga ttatattctt caagtgatgc
     4201 aatggattta acagctcttt ggcttaaaag tctaaaatca ccaacaccat catcaaattg
     4261 tacttcttct acaaagcaat taactaactt ataatacaaa tggcttaatg ttttgcgact
     4321 aaaattttca ccacttctat cacgctttgc aatcacttga tcatagcctt ccataaaacc
     4381 ttcaaccatt ttcggaatat attcaggtgg atgttgcaaa tcacaatcta tcatgatgac
     4441 tgcatcaaat tcagtactat gctggtaacc tgcaatcata gctgcttctt ttccaaaatt
     4501 tctactaaaa gaaataaagc tgacatgcct atctattgtg gcaagatgtt gcatttcatc
     4561 tatagtggta tccgtactac catcatctat gaaaagcata ttataatcat agcctttcac
     4621 actactatct tgtgaaagta tttcagttaa ttgttgatgt gtttgtgtaa tgacttcccc
     4681 ttcattaaaa caaggaatga cgactctaat tttcatctat gtccatcctt tttatttaaa
     4741 agattttgaa tgtacctgag tattgtttta tcaaatagta aaaaccaact catttatttt
     4801 aatgtacaca ttaaaaggca ttcataaacg taaaatttaa catccagtta ttttaaatcc
     4861 ccaattttca catcatctgg atttttacct tgtcttgcat ttctatttaa agcatcaatt
     4921 tcagctactt ctgttaattc taaattaaaa tctaaaatat ctatattttc ttgaatgcgt
     4981 ttaggtgtct gagattttgg aataataatt ctattgtgtg ctaaatgcca acgtaataca
     5041 acttgtgctg gtgttttatg atatttttca gcaattttaa caatgacagg gttgtctagt
     5101 agtcctctat ttctcatcaa aggcatccat gcagtcactt taatatcgtg acgatcacaa
     5161 aattcttgta attcttgttg gttaaaatat ggatgtacct caatttgatt caccattggt
     5221 ttgatacttg attgagccat taatttttct agatgatgaa cattaaaatt acatacacct
     5281 attgctttta ccttaccttg ctcgtaaagt tcttccatag ctttatatgt ttctaaaaat
     5341 agaccatctg cttcacaagg ccaatgtatt agaaataaat caagataatc agtttgtaaa
     5401 ttttcaatcg atttgttgaa atattcgaat gttttctcat aaccttgata gtcattccat
     5461 aacttcgttg ttataaacaa atcttctcta tcgacgccat tatcctttaa tgctcgtcct
     5521 agtgaagcct cattatcata aaagtatgct gtatcaaacg ctctatagcc tgcgtcaatt
     5581 gcagcattta caactttagt catatcttcg tcagagattt tataaacacc taatccaact
     5641 gaaggcatcg ggtatccatt atttaatatt tgtatctcat tcaacatctt tatatctctc
     5701 caatctatgt atcttttata tctttacatt accctaaatt tttcaataaa ctcaattaat
     5761 acgaattatc gctttcaata aaaaatattc attaaatcat taaagatatt gagttccaat
     5821 actattttca cttttcatta attttagtca aaaaaataac caaccaaaaa tgaattaaat
     5881 cattcttagt tggtttatat attaatatct attctgattt ttcatcttca tcagactgtc
     5941 cgatagtagg tctcgcttca ttaaattcat agtttaatat cacccaaata atttggtggt
     6001 tatcgatttc tgaaacaacc cagcgatcat aagttgtatc cacgtaatca tctttttgta
     6061 aattggtatt acgagattgt aaccatccac ctatcgtatc aatatcctca gagtcatcaa
     6121 attctatacc gaactcttca gttaaatcat ccaatagtac tctgccattt acttggaatg
     6181 tcttattatc aattttaacg atatcattca cttcatcatc atcaaattca tcacgaattt
     6241 ctccaacgat ttcttctaaa atatcttcca tcgttaaaat acctgccgtt ccaccatatt
     6301 catctataat aagactcata tgtacatgtt cacgttgcat tctaattaat gcatcactga
     6361 tacgtgttgt ctctgaaatc attggcaact catgtatata gtttgctatt ttaatcgttt
     6421 ttccagaagc gtattcagtt aaaaattctt tgacgttaat aaatccttta atgtggtctt
     6481 tatcaccatc atcagtaatt ggataacgcg taaattgatg ttcttttatt gtttctagta
     6541 attcgtctac attaaaaggt tcatttagtg taatcatttg agttctaggt accattatat
     6601 cttttgcatg tctttcatcg aatgaaaaga tattttgcat atatgccaat tcagtttggt
     6661 tgatttctcc accattataa ctattgttaa taataatttt gatttcttct tctgacattg
     6721 catcagtttg ggcatcagga tttacaccaa acattctaat aataacacgt gcagaaccat
     6781 tcatcagcca aatcaatggt ttcataatgt taccgaaata gaacaatggt cttgcatata
     6841 ctaaagcaag cttttcagta tgttgaatcg ctatagattt aggcgctaat tcaccaagta
     6901 ctacatgcaa atacgtaacg attataaatg acactgcaaa cgaaatcgtc gtcgttaatg
     6961 cagttggtaa attgattgct tcaaatattg ggtgtaatag cttttcaaac gttggttcac
     7021 caagccaacc taaccctaaa gatgttactg ttatacctaa ctgacaagca gaaagataat
     7081 aatctagatt agcaatcatc ttttttacta ttttagcagg tttatttcct tcatctgcta
     7141 gctgttcaat tcttgttgct ctaattttta ctaatgcaaa ttctgaacca acaaatacag
     7201 tggttaatgc aattaataga ataaatataa tcaaactaat tatggtcgaa gtttccaatt
     7261 aattccctat ttctagggat tcacctccat gttatatgtg tcactcatgg gtaacacgca
     7321 ttcaaattta tcactatgac ttaaattaca actacatgtt atcgccttcc catttagaca
     7381 cccccagaaa aaaatgttat tgcttctatt ttatcataat ataaagtgct tatgttaaca
     7441 gattaaatct attgcataca tttaattatg attatttatt agctaaatgt gtgaacctta
     7501 ccttgtttta ttgtagattt tacattcata tcatcatcta gaataacaag atctgcatcc
     7561 ttatttactt taatactacc ttttctatca tcgataccta atgcaatggc ttgatttaaa
     7621 cttgttactc gccataaatg atctaatgta tcacctgtaa atgatattaa gttacgtaac
     7681 ccatgattca tttttaaaat actaccagca agcgcaccat ttgcaagacg tgcttgttgc
     7741 gattgaacag ttactttttg tccacccaaa tcatattctc cttcaggcat accttttgca
     7801 cgcattgcat cggtaattaa ataaaaacgt tcattacctt tcatacggta agcaatagca
     7861 accgatgccg gatgagaatg tgtgccatca acaatcattt cggtatgtag agcatcattc
     7921 aaccatgctg ctccaaaaac acctggttct ctatgctgga atggcgtcgc tgcattatat
     7981 aaatgcgtga catgtttagc tcctcgctca acagcttcga ctgcttcttc gtatgttgcc
     8041 actgtatgac caattgaaaa aataatgtca tctttatacg tttcaagcgc ttcttttgca
     8101 ccttcaactt caggtgcaaa cgtcataatc tttattaatc tgttagcagt ctcttgaaaa
     8161 tgtttaattt tatcgataaa tgggcgtaca acgtattgcg gatgttgagc accaacttta
     8221 ttttcagata taaatggtcc ttctaaatgt atacctacaa tttccgctgc attgtgaaca
     8281 tcttgctccg cttcatattt agcaatattt gtaagtgctt tatctatttt atcagtagat
     8341 tgcgtcattg tagtggccaa gtatgatgtc gtcccttcag ataacaaatt ttcggataga
     8401 tattttaagc catcgtatga cccatccatt gcatcttgac cataaccacc atgaatatgt
     8461 atatcaataa aacctggtaa tacatgatga cctttagcat caatcacttg aattttattt
     8521 gtcgtatcat tatcaattgc tgctttatca tccccttctc caattgcaac aatctgtcca
     8581 tctttcacat gaatgtaacc attatcgatt ttgccatctt cagtataaac tttgccgtta
     8641 tatataatta attctgacac gtataaaccc ttctttattt ataaattttc actttaagac
     8701 actcatattt tactatatct gcttcgtttg gcataacttt ttgcaaaata ttaaaaaagt
     8761 ctatacattt gtggtgtcta tttcttcatt catttatcta aaaataaaaa caccttcata
     8821 tcaattcata aaaactgaat taatagaaag tgtttttaca gctttgtata agctaccgtt
     8881 aacacgatgt ataaaactaa catatattca ttgaacttac aacttaatat gaagctcttt
     8941 gctaacaatc attttacatc attaaaacta ctcgtccatt gattttgaag cttcgatttc
     9001 tgtttcagtt aacttgggtt tgattaaacc gtaaattaat gctgaaacta atgtaccaac
     9061 tagaagtgca ataagagttt gaagtaagtg tgcaccatcc gtaccaacaa ttacaataat
     9121 accaccatgt ggcgcagtaa ttcgtgaacc taagcctaaa gcaattgcgc cacctatacc
     9181 tgaaccaatc atcattgaag gaataacacg taatggatcg gcagctgcaa atggaatcgc
     9241 accttctgta ataaaggaca tacccatcac atagttaggg ataattgaac cacgttgttc
     9301 ttttgtaaat ttacgtctga aaattaacat cgctgtcgca attgctaacg gtggaatcat
     9361 accaccaatc attgcagctg taattggtgc tgcattacct tcaatcaacg cacctgttgc
     9421 aaaaacatat gccgctttgt tgaatggacc gcccatatca attgccatca tagcgccaat
     9481 aactaaacct aataatacaa tattagaacc tgataaattg tttaatccat ctaataacaa
     9541 atgatttaac caagatgctg gtggattaaa gacataaatc atcaataagc ctgtagctgt
     9601 cactgtcaat agtgggtaaa ttaatgttgg ttttaatccc tctaatgctt gtggtaactt
     9661 acgtgtcatc gctttaacac cttgtgttaa gtaacctgct aagaaacctg caataatacc
     9721 accaataaat cctgaaccac ctgaaattgc taacatacca cctacaagac cagctgcaaa
     9781 accaggttta tccgcaatac tacgtgcaat gaatccagat aaaattggaa tgattaacgc
     9841 gaatgcactt ttactaccaa tgttccatag ctgctctgca aacgcattgt actctgagct
     9901 ttttggttta aatgagtcta ctccaaataa aaatacgatt gccattaaaa taccaccaga
     9961 tattacaagt ggcaacatgt tagaaacacc gttcattaag tgtttataga atgctttacc
    10021 tgggcttaat ttttcttgac tgtcatttga gttacctttt ctttgaccat cacgggcaac
    10081 aaaaggttta cgacttgtat ctaatgcttt attaattaat tcttctgggc gtttaatacc
    10141 atctgctact ggtacttcta cgacattttt accatcgaag cgatccgtct caacatgaac
    10201 atcagcagca acaatgatac ctgttgcatt ttcaatatct tgttcagtta aatggttttt
    10261 aatgccgctt gaaccattcg tttctacttt aattttaata cccatcttat ccgcttgttt
    10321 tttcaatgcg tcacgtgcca tgtacgtatg tgcaatacct gttggacaag cagttactgc
    10381 taacacatat ggctcattag attgtttaga tgattgttct gcagttgcaa cttgttgtgc
    10441 ttcagcttct gcctcttctt cttttgtcac ttcatcatca gcctcatcta tgatcgctag
    10501 tacttcttca ggtgatgaag catgtaataa tttctcacgt acattttcat ccattaaaat
    10561 accagacaac ttagctaaag catctagatg tgtttgggcg ccaccttctg gcgctgcaat
    10621 cataaagaat aagtgtgctg gttgcatatc caaactttga taatctacgc ctgctttaga
    10681 tttaccaaac gcaatagctg gtgacttaac tgcggccact ttggcatgtg gaatggcaat
    10741 accttcgccg ataccagttg tactttgtga ttctcgattg tgaatcgctt ccttaaatga
    10801 cgcgacatca cttaatttac ctgctttgtc taattgattt actaactcat caataacacc
    10861 atttttgtca tttgccatta aatccattgc tattgtatct tttgttaata actctgttac
    10921 tctcattatt ttcactcccc atcaagtacg ctaatcgtaa cttgtgattt tattttttct
    10981 atagcgtccc gtgttgctaa gtcctcatca aatgccgtgg cagtaccgca tgcgactgct
    11041 tgttggaatg ctttttcaat cgttaaacct gaagcaattc cagccaccat gcctgcaact
    11101 gtactatcac cagagccaac tgtattaacc actttccctt gtggattaac tgctttaata
    11161 ctgatttctt tatcaatata aatagcacca tcaccgccaa gcgagacaat aacagattgc
    11221 gcacctttat caactaacaa acgaccatat ttaataacat ctgtgtctga gttcactgtt
    11281 gtattaaaca tcacttctaa ttcatcttta ttaggtttaa taaatagtgg atgatatggt
    11341 aaaacgcttt cagccaattc tttttcagcg tcgactacta atttagcacc tgtctgtgct
    11401 gtaatttgtg caatttgcgc atacgcatcg cttggaatac tacttggtac acttccagca
    11461 acaataacta tatcttcgct tgttgtattt ttaatttgtt gtaacagttg ttcaaattgt
    11521 gttgacgtta tatgaggacc cggtgcattg atttctgttt cttgtcctgt ttttaatttc
    11581 acattaatac gtgtatcttc atcaacttca ataaaattcg attgaattgc actgttattt
    11641 aatgtatcta taatgaattt cccaggaaat ccacctgcaa atcccaaggc agttgactca
    11701 acatccaatg tctttaagac gcgcgagaca ttaatacctt tccccccagc gaatttatat
    11761 gttgctgttg ctctgttcaa accatcaatt ttaaaatcat tcgtaaaaat gacatagtca
    11821 attgaaggat tgaaagtcac tgtataaatc ataaagtccc tcctataaag tgatactttt
    11881 gttggtattc ttttaacgat tcttgattta atgctttttc agatgtgatg attgtcgtac
    11941 tttctagcaa aggtacacga gcaaaatata ctttattaaa cttagaatga tctataagta
    12001 caaatgattg attggctaat gacattgctg tttgtttaac taatgcctct tgctcatcgg
    12061 gagtagttaa tccaagttca atatctaatc cattcatccc gataaaagct ttatcgaaac
    12121 aatatcgtct taatatctcc atagcactag aaccaatcgt agcaagtgta ttttctttaa
    12181 cttgaccacc tagcataatt gttttaatac cttttttaag taaagcttct acatgtgtta
    12241 aaccattggt taccacaatg atatctttcg cttgaatata tttaattagc tccaatgtag
    12301 atgaaccagc atcgataaat aagcattcat tatcgttgat ttgattagct gctattttag
    12361 caatcatttt cttttcatca agattcgttg ctaatttttc agttaaattc gcctcaacca
    12421 tacgattttc ttttaacatt gcaccaccat gcacacgttg caatttccct aattgttgta
    12481 gtttagataa atctcttcgt attgttgaag cactgcatcc agttcgatct attagttctt
    12541 gtaaagtcaa aaaatctttg tgcgaaagtt cttctaatat taactcgtgt cttttttctg
    12601 taattatcat catcacccca tctcactctg tattataatg agtacgcttt cacttttcaa
    12661 tcataaatta ccaaaaacaa tcacaaacaa tcaaattgtg acgataaaat tacattttta
    12721 tgttcaaata ctcatttgta atgactgttt atgatcgtaa aatgctaatt caatcaaaaa
    12781 ggagtgtggc agaaatgaat tttataaaaa tttcatctct gtcacactcc gaatcttgcc
    12841 ccatactttt tgttgttatt aatcattcat ggataactgc tccaattttg cctttagtta
    12901 ttgtaatcaa atcctcaaca gctattgtga tttgcattgt tcgaagccca ccactcacac
    12961 tgatatgact ataattttca cacgatttgt caacgactgt tggaaacaat gttttcatac
    13021 caacaggcga acacccacca cgaatgtatc ccgttacatt tttcaaatta tctaaaggca
    13081 taagctgcaa tttcttctct ccaaccaaag cagctgcctt tttcatatct aaagtttcac
    13141 ttactgggat aacaaataca aaatgttcat gttttgtatt ttctagaaca agcgttttaa
    13201 atactgtttt aacatttgct tgtatgagtt ctgcgacttc ttgaccagat aaatgttctt
    13261 ctggtacctc aaagctatga acttcgtatt taatttttgc acgatcaagc atacgcatcg
    13321 cgttcgtttt cttattttta gccatttcaa cacctcattt atacttcttc agaactatat
    13381 taacatttta aaatacatta cggattggtt gattttgaca taacatttac atcattttat
    13441 aagttgattt caccttttta aattaaatta ttgcaaaata aaaacagtat atccaattag
    13501 aatacactgt tccattatta tttcgttatt atgccttgat ttgaaatact tacatgttgc
    13561 ttactagttg aattatcatt ggaataaaca taattatatc catgatttgt cttagaaatt
    13621 ctttctaaac cataattatc attgatatgc ttatctgcat aacgatcagc aataacttgg
    13681 cactcttgtt cgttaaaaga atttgattta tttgcattca cacgatgttt aaaatgattt
    13741 tgctttttca tattttcatt cacttcagat gctttagcag tatgaatatt gagaatatcc
    13801 attttctttt tataagtatc ttcagttgat acatttatcg cgttaagcgt attaattgtt
    13861 ttatgtacct tagcaagcgc atcttcattt gttgctaata ttaataaacc aattaaacaa
    13921 acggttaata ttacagcaac aaagcctaaa aataaatttt ttactgtcat ccccttaccc
    13981 acatttcctt actcattttt tatcatctta acacattaac gttaatgaat taggtatgtg
    14041 gattgcaatt taaaaagaat gtaatatttg taacatgttt attactcaaa atcatttttt
    14101 gttacatata acatttaaat ataaagagta aaacatttag tcaatcgaat ctttttaaat
    14161 gtaaagcatc gaattttctt aataaaattc ataagaaaaa cgatgctaat cattcataat
    14221 attacgcttt actttatatc aatatacacc aaaatactta tgctacatat tttgttcttt
    14281 caattttgct ctatgttgct tttcaattaa aacaataaca acacctgcta atgaaacacc
    14341 tatagccata taaattggtg cttcaatgtg tacatcaaat aacgcacctg cgattaaagg
    14401 acctatgaaa ttacccatac tagtgaatgt cgagttcaat ccgcctgcaa agccttgcct
    14461 ttctccagca atattagaaa aataatttgt aatggctggt cgtatcatat caaaacctat
    14521 gaagacaaca aaactgatta acattattga ccaatagcca ttagcaaaaa ctaataatat
    14581 taagacaaca actgaatata ataatgacca agctataaat gttaactctg agaaatactt
    14641 cataaattta tcgaagaaat agatttggaa aagtgcccca aatataccgc cacccgtaat
    14701 agcaatcgaa atatctttag gtgaataatt taccttgtca gctgtgtata gtgaatacaa
    14761 tgtttcaaat gcagataaac caaacgataa tacaagtgtt aaaataactg gtgtaataaa
    14821 cactttccag ttaattttcg ttagcaattg tggctctaac ttttggaaac cacttgtcgt
    14881 agactttttc ggatcgtgaa tcaatacaat tgacattata aatgctagaa tacctaatgc
    14941 tcctgcaaag taaaatggca tacgatgtga aacttctgcc ataaatccac caatccctgg
    15001 tcctaaaatg aatccagaat tgataatcgc tgacatgtag ccaaagtttt ttgctttttg
    15061 atggcttggt gaaatgtcag ctattaaacc tgtcacacca ggcattacca taccagcact
    15121 cataccacca atcactctcg ataacatcaa taccgaaaaa ttgtggccaa ctgcaaacat
    15181 aaattctgac actgaaaaca aaattaatcc tatacatata attaatttct tccctaattt
    15241 gtcagctagc gtaccaccaa acggcgatat aatcatttga gataacgcaa aagcagcaac
    15301 tagtaatcct aaatcactac cagttaatcc caaatctttt aaataaacag gcaagactgg
    15361 tattactaaa ccgataccta aaaaaatcaa gaaaatatta aaatataaga caaaaatctg
    15421 tttattcata tgctcacctc ttttttctcc atgtcatgct taaagctgtt attcttcttt
    15481 tccacgacag attgcaattc atgctaaata ttcgtaaaaa gtttctatat tgtattacaa
    15541 cattgctaca tttgacaata ttttttcttt gtaattatca ctattttcca tttaattgta
    15601 taattaatga catattaaaa taaaggggat tgatcatatg aaattgcaaa aaagtaatca
    15661 tactatacta ctagtgttgg aaaaaggaga agatatcgtt gaatgtataa caacctttgc
    15721 agatgaccaa gacttaacat ttacatctgt cagcggcatt ggtgcatgtg atgacgttgt
    15781 attaaaattt ttcaatttaa caacgaaaca atacgaagaa aaacatatta ctgagccact
    15841 ggaattgacg agcttattag gtaatatttc tcgtctagac aatggtcatt ttgcacattt
    15901 gcatgctact ttcggtactc aatcatatga aacttttagc ggacatctcg ctaaagcaat
    15961 tgtttctgca actgctgaaa tcattcttac tgtaactgat ttggacattc aacgttcatt
    16021 caaagatgca gtcggtttaa atttacttga tcctcaatag cactttactt tttagttgaa
    16081 tcaaatttta ttacaagtta tcataagtgc ttaatcataa atgcattctc gtttattaat
    16141 atacttcaac gagttaaatt aaatttttaa ttaggaattg aatgcgttta taactatcac
    16201 atttgaatga ataacttttt cattagaaag tagtgtttat tattgatgca atctcgatac
    16261 atcgctagaa tttacttttt aattttattt atcgtctccc tttttgagac taaaattttt
    16321 caatttatga ctttaaattt gtttttagca tatatacctt ttgagcttag tctattatta
    16381 cgattattca aaccgaataa aaagtatgaa tggccactct ttatcatata tagttttata
    16441 tttgtgttac ttttaccaaa tacattttat atggtaactg atttaatcca tttgaaccaa
    16501 tttcaattca acttttacgc aggactaaat ttaagtgagt ggaaatactt tacctattta
    16561 ctgcttggtg tcttcttagc ggtttatgtg atgattttaa tctattttga aatactaaca
    16621 ttgacttctc accgttggct taatagatta attgttgtcg tgctaatgtt tttaaatgga
    16681 ttcggaattt atattggtcg tttcttacgc ttacattccg tttatttctt taatgagccc
    16741 ttacatgttc taaatcacat tctagaaagt ctaacgctta aaactgctat gtttgtatgt
    16801 tttatggttg tcatgcaagc agcaattcta ttatttggaa aaggagtgcg tttgaacaag
    16861 tgacaataaa tataatcgtt ttaatagtat ctatcattgt ctttcaattg attattggac
    16921 acatatggca tgatattggt ttaagttatt taagaagtat cttactcatg atgctgccat
    16981 ttggactagg tgtatttata caacaagtgt cgtattacga acgacaatat cctaaatggc
    17041 aagtgcctca aaacataaaa gttagattga aatatattta tctcgctacg tttttagagt
    17101 atgtcgtact atacctcact ttatttacgg acatactaag ataacatcaa attaataaga
    17161 aaactaaata caacttatat atttgaggaa agccaatttc tatcacatca ataatagaaa
    17221 ttggcttatt tatattgctt aagaacttta tattctgctc cagattttac tcatgccaga
    17281 tctatcaatt gagccgaatt agtctagcgc tttaaatgta gctaaaactt gtgtacgttt
    17341 ttcttgatga tctaccattt gtcttggata atgactacct aattcaatcc cctgttcaaa
    17401 aaggttggac tcattgcgtt gtgtatcatg caaatatttt gcatcaattt gattaaaaat
    17461 cggaagatat gttttgatat acaaagcttt agcatcaaag cgttcactct gtcttattgg
    17521 attaaacatt ctaaaatacg gcactgcatc cgtacctgta gaagcagacc attgccatcc
    17581 gtgaatattt gatgctgcat catagtcaat aaggtgcttt ctaaagaatt tttctcccca
    17641 tgtccaatct ataaataaat ctttggttaa aaattgcgac acaaccattc tcattcgatt
    17701 atgcataaaa ccagtttgtg tcaattccat tattgctgca tcaatgattg gaaatcctgt
    17761 ttgcccttcg caccatgcat taaaatccgc ttcattttgc gaccatttta tctgtcgata
    17821 tttaggtttg aaagcttggt atgaggtttc aggatactgt gtcattaaca cataataaaa
    17881 ttctctaaaa atgagttcac gtataaatgc ttcatagttt gcctcatctt catcataacg
    17941 cgccaataaa tcattaaaaa tttcaataat atctaataat ccatatgcta aagcaacact
    18001 tagctgactt gttaatactt caggtaaata gtctcttccg ctttcgtaat tctgtatatc
    18061 ttgatctaaa aaatgttgcc atttattctg ttcaattatt tgtgaggatc caaatgctat
    18121 gtcatcaaac actaaatcat caggtgatgc aatgacaaag tcttcgaatg attttaaatc
    18181 atagtgatat acgtctctat gcctcaagta aggtcgccat tttttataga aactagtaaa
    18241 aactttgtat ggctcccctt gttgattcat tgttttactc gctttaaagt aatgattccc
    18301 acgtatcaaa gtaacggcaa tgcgtgcttc attgaataaa gaacgttgat gtaaaatatc
    18361 atattcttca agatgataac tcataatatc acccgcagtc actacttcag agatattgtt
    18421 ttctttacaa aatgaaatga tatcgtcata agacatgaca taaggtgtga gcttttctgc
    18481 gtgcaacgat tggtaaaagc gctgcaaagt accgcgataa aactgtgctt tcaaatcaga
    18541 agctgaagac atgtcctctt ccggaatgat aaaataacat gcatcaatgt cttctttttg
    18601 ttgataaata tattcaaata atggattgtg ttccattcga aacattcgat ttaataacac
    18661 agcaattgcc ataaacaaaa cacccttcaa ttatcttttt attttattaa gctttttgtc
    18721 actttaaaat aacctagatt cttatttata tttttaatta aagcaaacct aaccatttcc
    18781 aataagtcat gctaaaaact atcactagaa tataccctac aattgtcaat ggtatacctg
    18841 ccttcaagaa atctttaacc gtaaaagtac cagtgccata agccaacata ttttgaggtg
    18901 cactaacagg taataagaaa ccaaaactaa taacaaattg ttgaattaaa acaaatccta
    18961 tagactggtc tcctaagtgt aacgtagagg ttagcgaaat aaaaacaggt attaacgctg
    19021 atgataaact tgttgcactc gcaaagccca aatgaatcaa tatattaaaa agcgtgataa
    19081 gtgcaattgt cgcgataata ggtaaatgtt ttaaacctaa aacaccaaaa gtttgatcac
    19141 ttaaccattg agctgcacct gttttcaaaa gaacgttccc tagtgaaata cctacaccaa
    19201 acacgataat tgttccccat ggtattttat tatcaacatg tttccatgtc atgacaccaa
    19261 ttttcggcat taacataaca cctaaagcaa taatagtaat ggatgcagag tcaatcggat
    19321 gtaatacttt ttcagttgac caaaacagta ataataacat cgatataaca attaaacgcc
    19381 attcacgtgg gctaacgggg ccaagtttat gcaattcttc ttttattaaa tctttaccac
    19441 cttctattgt attaatttct ggaggcatca ctttaatcat gatgaaatat aaagctacgg
    19501 acataactat ggaccaaggc gctgcatata agaaccactc gccccatgaa acatcaaatc
    19561 ctaattgatg gtttataaaa ttaatcgcta cgatattttg tgctgccgcc gttttgatac
    19621 caatattcca aattgacaca gcttgtactg aagtaattat taataaagac gctaacttgc
    19681 tatctttgga aactttaaat gccgcaatca tacccagcaa gattggtaca actgcccctg
    19741 ctctagctgt tgcagaagga acgaaaaatg caagtacaat tgaaacgata attgctccaa
    19801 taactatatt tctagtttta ttaccaacaa ttgataacac taaaagagct agtcttttat
    19861 gcaaattcgt ttcttgcata gcagcagcca aaaataatgc agctgcaacg agagctacag
    19921 ctgaagttgc atatccacta aacgctaatg ataatgcatg attagttcct agaaggtcac
    19981 ttccagctaa aatagcactg ccacttttcg gattacctag cttctcccct aaattttgaa
    20041 ccgggctaaa tcctaaaagt aatatcatta agccaataat taaagttgct gtcaccggat
    20101 atgatacagc ttccgttacc cacataataa ctgcaaaagc taaaatagct agtactgcct
    20161 tagccattat cggcagactt gaaggtgccg ggagtaatag tacaactata agtattataa
    20221 aactcatgac aatccaaaat ggcttgtatt gcttttttgt cttttctttc ttttgttgtt
    20281 catgttccac tacatgtttt tcttctgaca taacagtcac ccctttgttt aatcttaatt
    20341 taatta
//
LOCUS       DAMZLE010000034        20341 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMZLE010000034 DAMZLE010000000
VERSION     DAMZLE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.034"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            515..1810
                     /gene="purB"
                     /locus_tag="POB92_001116"
     CDS             515..1810
                     /gene="purB"
                     /locus_tag="POB92_001116"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI6578625.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1919..2221
                     /locus_tag="POB92_001117"
     CDS             1919..2221
                     /locus_tag="POB92_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578626.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2393..3085
                     /locus_tag="POB92_001118"
     CDS             2393..3085
                     /locus_tag="POB92_001118"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDI6578627.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3082..5274
                     /gene="pcrA"
                     /locus_tag="POB92_001119"
     CDS             3082..5274
                     /gene="pcrA"
                     /locus_tag="POB92_001119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDI6578628.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5278..7281
                     /gene="ligA"
                     /locus_tag="POB92_001120"
     CDS             5278..7281
                     /gene="ligA"
                     /locus_tag="POB92_001120"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI6578629.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7294..8493
                     /locus_tag="POB92_001121"
     CDS             7294..8493
                     /locus_tag="POB92_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDI6578630.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAVDYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8582..10120)
                     /gene="putP"
                     /locus_tag="POB92_001122"
     CDS             complement(8582..10120)
                     /gene="putP"
                     /locus_tag="POB92_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDI6578631.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10487..10789
                     /gene="gatC"
                     /locus_tag="POB92_001123"
     CDS             10487..10789
                     /gene="gatC"
                     /locus_tag="POB92_001123"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDI6578632.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10791..12248
                     /gene="gatA"
                     /locus_tag="POB92_001124"
     CDS             10791..12248
                     /gene="gatA"
                     /locus_tag="POB92_001124"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDI6578633.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12261..13688
                     /gene="gatB"
                     /locus_tag="POB92_001125"
     CDS             12261..13688
                     /gene="gatB"
                     /locus_tag="POB92_001125"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDI6578634.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13728..13874
                     /locus_tag="POB92_001126"
     CDS             13728..13874
                     /locus_tag="POB92_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578635.1"
                     /translation="MIADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            14342..15289
                     /locus_tag="POB92_001127"
     CDS             14342..15289
                     /locus_tag="POB92_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6578636.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            15370..16731
                     /gene="rlmD"
                     /locus_tag="POB92_001128"
     CDS             15370..16731
                     /gene="rlmD"
                     /locus_tag="POB92_001128"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI6578637.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            16901..17431
                     /locus_tag="POB92_001129"
     CDS             16901..17431
                     /locus_tag="POB92_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDI6578638.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPAQ"
     gene            17678..18748
                     /gene="dinB"
                     /locus_tag="POB92_001130"
     CDS             17678..18748
                     /gene="dinB"
                     /locus_tag="POB92_001130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI6578639.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(18815..19369)
                     /locus_tag="POB92_001131"
     CDS             complement(18815..19369)
                     /locus_tag="POB92_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDI6578640.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            19585..19677
                     /locus_tag="POB92_001132"
     CDS             19585..19677
                     /locus_tag="POB92_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578641.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(19759..20259)
                     /gene="ftnA"
                     /locus_tag="POB92_001133"
     CDS             complement(19759..20259)
                     /gene="ftnA"
                     /locus_tag="POB92_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDI6578642.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
BASE COUNT         7504 a         2923 c         3741 g         6173 t
ORIGIN      
        1 ctatgcaata gaacatatat tctttttgtg agtagaaagt ttgttatatt agaagtgaat
       61 aattttaaaa catattatgg aaatgtaaga aggagtatta tgaaactagg taaagtagat
      121 tgttgaaaat tccatacaat aggagaagta aagtgaataa aatctggtat tactaaagct
      181 attgattgtc ctaattgtaa taaaaagttg gatagtgtag ttgaggggag aaaaactaaa
      241 atgcaataat atattcttaa tggatcatta taaataccaa ttagattaaa gtattgcgaa
      301 catttacaat tagaaatcag tattaatctg ccatagataa ttgtattaat gtaaaaaata
      361 aaatagaatg agaatatacg gaaatttttt atacatttat aaccaattct gttaaactgt
      421 gaagagtaga gttaaaaacg aactttttgt ttatttttat taaaaatgtt ctgtttttga
      481 aaattcgagt atacatacga taggagttaa aaaaatgatt gaacgctatt ctagagaaga
      541 aatgtctaat atttggactg atcaaaatcg ctatgaagca tggttagaag tggaaatttt
      601 agcatgtgaa gcatggagtg aattaggaca tataccgaaa gctgacgtac aaaaaattcg
      661 tcaaaacgca aaggtaaatg tcgaacgtgc acaagaaatt gaacaagaaa cgcgccatga
      721 tgttgtagcc tttactagac aagtttctga aacgctaggt gaagaacgta agtgggtaca
      781 ttatggttta acttctactg atgttgtaga tacagcttta agtttcgtta ttaaacaagc
      841 aaatgacatt attgaaaaag atttagaaag atttatcgac gttttagctg aaaaagcaaa
      901 aaattataaa tatacattga tgatgggacg tacacatgga gtgcatgcag aaccaactac
      961 atttggtgtc aaaatggcat tgtggtacac ggaaatgcaa cgtaacttac aacgtttcaa
     1021 acaagtaaga gaagaaattg aagtagggaa aatgagtggt gcagtaggta cttttgctaa
     1081 cattcctcct gaaatagaaa gttatgtgtg taaacattta ggtattggca cagcgcctgt
     1141 atcaacgcaa acattgcaac gcgatcgaca tgcatactat attgcgacat tagcattaat
     1201 cgcgacgtca ctagaaaaat ttgccgttga aattcgtaac cttcaaaaaa ccgaaacaag
     1261 agaagttgaa gaagcatttg caaaaggcca aaaaggttca tctgcaatgc ctcataaacg
     1321 aaatccaatt ggttctgaaa atatcacagg tatttcaaga gtcattcgtg gttatattac
     1381 tacagcttat gagaatgtgc cattatggca tgaaagagat atttctcatt cttctgctga
     1441 gcgtattatg ttaccagatg ttacaatagc actagattat gcattgaatc gtttcacaaa
     1501 tattgtggac cgtttaactg tatttgaaga taatatgcgt aataacatag ataaaacatt
     1561 tggtctgatt ttctcacaac gtgtcttatt agcattaatt aataaaggta tggttcgtga
     1621 agaagcatac gacaaagtac aaccaaaagc gatgatatct tgggaaacga aaacaccttt
     1681 ccgtgaatta atcgaacaag atgagtctat tacaagtgtt ttaacaaaag aagaattgga
     1741 tgaatgtttc gatcctaaac atcatttgaa tcaagttgac actattttcg aacgcgctgg
     1801 attagcataa aattaaataa agtaacgatt cagatttaat aaaatataaa tcgctacact
     1861 ttaatagatt aatgcgttaa aatgacataa agaataaaat tataggggtt gtttcgatat
     1921 gcaaattgaa aaattacgag gtgcagcgtt agatgaattg tttgatgcaa tactaacgtt
     1981 agaaaataga gaagaatgtt accaattttt cgatgatttg tgtactgtaa atgaaattca
     2041 atcactgtct caaagattac aagttgctaa aatgattaag caaggttata cctatgcaac
     2101 gattgaacaa gaatctggag catcgactgc aacgatttct agagtgaagc gttcattaca
     2161 atggggtaat gatgcttata caatgatttt agatcgtatg aatattgaaa caaatgaata
     2221 ataaatgaac gataaacaat ggttatctat ctgcactaat aaggtagata atcattgttt
     2281 tttcacgaaa aaatttacag agtaaaagaa cttaaatttc atattaagtc tttagaactc
     2341 gacacttaaa aatgctataa tcatatgtat gttaaaaaag gagtttcgga aaatgtatga
     2401 cattaaaaaa tggcgccata tttttaaatt agacccagct aaacatattt cagatgatga
     2461 tttagatgcg atttgtatgt ctcaaacaga tgcaattatg attggtggaa ctgatgacgt
     2521 tactgaagat aatgtcattc atttaatgag cagagtaaga agatacccat tgcctttagt
     2581 acttgaaata tcaaacatcg aaagtgtaat gcctggtttt gatttttatt ttgtacctac
     2641 agtactgaac agtacagatg ttgtatttca caatggtaca ttattagaag cgcttaaaac
     2701 atatggacat agtatagatt ttgaggaagt aatatttgaa gggtatgtcg tgtgcaatgc
     2761 tgatagcaaa gtggcaaaac ataccaaagc aaatacagat ttaacaacag aagatttaga
     2821 agcatatgcc caaatggtca atcatatgta tcgattaccg gttatgtata tagagtatag
     2881 tggcatttat ggcgacgtat caaaggttca agctgtctca gaacatctaa cagaaacgca
     2941 acttttttat ggtggcggta tttcctcaga acaacaagcg acagagatgg cagctattgc
     3001 agatacaatt atcgtcggtg atattattta taaagatatt aaaaaagctt taaaaacagt
     3061 aaaaataaag gagtctagta aatgaatgcg ttattaaatc atatgaatac agagcaaagt
     3121 gaagctgtaa agacaacaga aggaccattg ttaattatgg caggtgctgg ttcagggaag
     3181 acacgtgttt taacacatag aattgcttat ttattagacg aaaaagatgt ctcaccatac
     3241 aatgttttgg ctattacttt tacaaataaa gctgcaagag aaatgaaaga acgtgttcaa
     3301 aaattagtag gtgatcaagc agaagttatt tggatgtcaa cattccactc aatgtgtgtt
     3361 cgtattttac gtcgtgatgc agatcgaatt ggtatagaac gcaattttac gataattgat
     3421 cctacagacc aaaaatctgt tattaaagac gtcttaaaaa atgaaaatat tgatagtaaa
     3481 aagtttgaac ctcgtatgtt tatcggtgcg atcagtaatt tgaaaaatga acttaaaaca
     3541 cctgcagatg ctcaaaaaga agccacagat tatcactcgc aaatggtagc aacggtttat
     3601 agtggatatc aacgccaatt gtcacgtaat gaagcgttag attttgatga ccttattatg
     3661 acaacgatta acttatttga gcgtgtacca gaagttctag aatattatca gaacaaattc
     3721 caatatattc atgtagatga gtatcaagat actaataaag cacaatacac attagttaaa
     3781 ttattagcaa gtaagtttaa aaacttatgt gttgtaggtg actcagatca gtcaatttat
     3841 ggttggcgtg gtgctgatat tcaaaatatc ttatcatttg aaaaagacta tccagaagcg
     3901 aatacaatct ttttagagca aaattatcgt tcgacgaaaa cgattttaaa tgcggctaac
     3961 gaagtgatta aaaataattc tgaacgtaag ccaaaaggac tgtggactgc aaatacgaat
     4021 ggtgagaaaa ttcattacta tgaagcaatg acggaacgtg atgaagcgga atttgtaata
     4081 cgagaaatta tgaagcatca acgtaatggt aagaaatatc aagatatggc aattttatat
     4141 agaacgaatg cacaatcacg tgtacttgag gaaacattca tgaaatctaa tatgccatac
     4201 acaatggttg gtggccaaaa gttctatgac cgtaaagaaa tcaaagattt attaagttat
     4261 ttgcgtataa ttgccaatag taatgatgac attagtttgc aacgtattat taatgttcca
     4321 aaaagaggtg taggtccttc atctgttgaa aaagttcaaa actatgcact tcaaaacaat
     4381 atcagtatgt ttgatgcact tggagaagct gattttatcg gattgtcaaa aaaggtgaca
     4441 caagagtgtc ttaattttta cgagttaatc caaagcttga taaaagaaca agaattttta
     4501 gaaattcatg aaatagttga tgaagtatta caaaagtctg gctatcgaga aatgcttgaa
     4561 cgtgaaaata cattagaatc tcgaagtaga ttagaaaaca tcgatgaatt tatgtcagta
     4621 ccaaaagact atgaggaaaa taccccatta gaagaacagt cattaattaa ctttttaacg
     4681 gatttatcgt tagtagctga tattgatgag gcagatactg aaaatggcgt aacactaatg
     4741 acgatgcact cggctaaggg ccttgaattt ccaattgtct ttataatggg gatggaagaa
     4801 tctttattcc cacatattag agcgattaag agtgaagatg atcatgaaat gcaagaagaa
     4861 cgtcgtattt gttatgtagc aattacaagg gctgaagagg tgttatatat cactcatgcg
     4921 acatcaagaa tgttatttgg tcgccctcag tcaaatatgc catccagatt tttaaaggaa
     4981 attccagaat cactattaga aaatcattca agtggcaaac gacaaacgat acaacctaag
     5041 gcaaaacctt ttgctaaacg cggatttagt caacgaacaa cgtcaacgaa aaaacaagta
     5101 ttgtcatctg attggaatgt aggtgacaaa gtgatgcata aagcctgggg agaaggcatg
     5161 gtgagtaatg taaacgagaa aaatggctca atcgaactag atattatctt taaatcacaa
     5221 gggccaaaac gtttgttagc gcaatttgca ccaattgaaa aaaaggagga ttaagggatg
     5281 gctgatttat cgtctcgtgt gaacgagtta catgatttat taaatcaata cagttatgaa
     5341 tactatgtag aggataatcc atctgtacca gatagtgaat atgacaaatt acttcatgaa
     5401 ctgattaaaa tagaagagga gcatcctgag tataagactg tagattctcc aacagttaga
     5461 gttggcggtg aagcccaagc ctctttcaat aaagtcaacc atgacacgcc aatgttaagt
     5521 ttagggaatg catttaatga ggatgatttg agaaaattcg accaacgcat acgtgaacaa
     5581 attggcaacg ttgaatatat gtgcgaatta aaaattgatg gcttagcagt atcattgaaa
     5641 tatgttgatg gatacttcgt tcaaggttta acacgtggtg atggaacaac aggtgaagat
     5701 attaccgaaa atttaaaaac aattcatgcg atacctttga aaatgaaaga accattaaat
     5761 gtagaagttc gtggtgaagc atatatgccg agacgttcat ttttacgatt aaatgaagaa
     5821 aaagaaaaaa atgatgagca gttatttgca aatccaagaa acgctgctgc gggatcatta
     5881 agacagttag attctaaatt aacggcaaaa cgaaagctaa gcgtatttat atatagtgtc
     5941 aatgatttca ctgatttcaa tgcgcgttcg caaagtgaag cattagatga gttagataaa
     6001 ttaggtttta caacgaataa aaatagagcg cgtgtaaata atatcgatgg tgttttagag
     6061 tatattgaaa aatggacaag ccaaagagag tcattacctt atgatattga tgggattgtt
     6121 attaaggtta atgatttaga tcaacaggat gagatgggat tcacacaaaa atctcctaga
     6181 tgggccattg cttataaatt tccagctgag gaagtagtaa ctaaattatt agatattgaa
     6241 ttaagtattg gacgaacagg tgtagtcaca cctactgcta ttttagaacc agtaaaagta
     6301 gctggtacaa ctgtatcaag agcatctttg cacaatgagg atttaattca tgacagagat
     6361 attcgaattg gtgatagtgt tgtagtgaaa aaagcaggtg acatcatacc tgaagttgta
     6421 cgtagtattc cagaacgtag acctgaggat gctgtcacat atcatatgcc aacccattgt
     6481 ccaagttgtg gacatgaatt agtacgtatt gaaggcgaag tagcacttcg ttgcattaat
     6541 ccaaaatgcc aagcacaact tgttgaagga ttgattcact ttgtatcaag acaagccatg
     6601 aatattgatg gtttaggcac taaaattatt caacagcttt atcaaagcga attaattaaa
     6661 gatgttgctg atattttcta tttaacagaa gaagatttat tacctttaga cagaatgggg
     6721 cagaaaaaag ttgataattt attagctgcc attcaacaag ctaaggacaa ctctttagaa
     6781 aatttattat ttggtctagg tattaggcat ttaggtgtta aagcgagcca agtgttagca
     6841 gaaaaatatg aaacgataga tcgattacta acggtaactg aagcggaatt agtagaaatt
     6901 catgatatag gtgataaagt agcacaatct gtagttactt atttagaaaa tgaagatatt
     6961 cgtgctttaa ttcaaaaatt aaaagataaa catgttaata tgatttataa aggtatcaaa
     7021 acatcagata ttgaaggaca tcctgaattt agtggtaaaa cgatagtact gactggtaag
     7081 ctacatcaaa tgacacgcaa tgaagcatct aaatggcttg catcacaagg tgctaaagtt
     7141 acaagtagcg ttactaaaaa tacagatgtc gttattgctg gtgaagatgc aggttcaaaa
     7201 ttaacaaaag cacaaagttt aggtattgaa atttggacag agcaacaatt tgtagataag
     7261 caaaatgaat taaatagtta gaggggtatg tcgatgaagc gtacattagt attattgatt
     7321 acagctatct ttatactcgc tgcttgtggt aaccataagg atgaccaggc tggaaaagat
     7381 aatcaaaaac ataacaatag ttcaaatcaa gtaaaagaaa ttgctacgga taaaaatgta
     7441 caaggtgata actatcgtac attgttacca tttaaagaaa gccaggcaag aggactttta
     7501 caagataaca tggcaaatag ttataatggc ggcgactttg aagatggttt attgaactta
     7561 agtaaagaag tatttccaac agataaatat ttgtatcaag atggtcaatt tttggacaag
     7621 aaaacaatta atgcctattt aaatcctaag tatacaaaac gtgaaatcga taagatgtct
     7681 gaaaaagata aaaaagacaa gaaagcgaat gaaaatttag gacttaatcc atcacacgaa
     7741 ggtgaaacag atcctgaaaa gattgcagaa aaatcaccag cctatttatc taacatttta
     7801 gagcaagatt tttatggtgg tggagataca aaaggtaaga atattaaagg tatgacgatt
     7861 ggtttagcta tgaatagtgt ttattactat aaaaaagaaa aagatggacc gacttttagt
     7921 aaaaaactag atgatagcga agttaaaaag caaggtaaac aaatggctag tgagatatta
     7981 tcaaggttac gtgaaaatga tgatttaaaa gatataccaa ttcattttgc aatttataag
     8041 caatcaagtg aagattcaat cacaccaggt gaatttatca ctcaagcgac tgcagaaaag
     8101 agtcaaacaa agcttaatga atggcataat atcaatgaaa aatcagcttt attaccttct
     8161 tcaacagcag tagattatga tgaaaattta aataataatt tcaagcaatt taatgataat
     8221 ttgcaatcat atttttctaa tttcacacaa gcagtaggga aagttaaatt tgttgataaa
     8281 aagccacaac gattagtagt agatttacca atcgattact atggacaagc tgaaacaatt
     8341 ggtattacac agtacgttac tgaacaagcg aataaatatt tcgataaaat cgataactat
     8401 gaaattcgta ttaaagatgg taaccaacca cgtgctttaa ttagtaagac aaaagatgac
     8461 aaagaaccgc aagttcatat ttacagtaat taattgtaat ttaaagataa taaaaaggta
     8521 ccaatgttag tcagaatttt gattaacatt agtacctttt taatttgtta agacttatga
     8581 attatttttc tctaacgatg tcgcggactt tgtttaagtc agtttcaaca aatgcaccag
     8641 gttttttagt aagtttactt acaacatatg taacgattac acttacaata aatccaggaa
     8701 taatttcata taagccgaat atttcgttga tatgtgccaa tggtttaatc cacgcaatcc
     8761 aaacgataac gactaaggca cctgaaacca ttccacttac agcaccggca cgtgtcaaac
     8821 ctttccagta aagtgcaaat agcacaagtg gactgaacga tgcaccaaat ccggcccaag
     8881 cgttacctac taagtttaga attgtgtcgt ttggattcca tgcaatcgcg atggcaacaa
     8941 ttgctacaac taatacagat aatcttccaa tcataacaaa ttctttttgg tgcgttttag
     9001 ctttttcttc accacgaatt aatttataaa agtcttccgt tagtgaacta gatgttacaa
     9061 gtaattgtga agaaatcgtg ctcataattg cagctagaat cgcagcaagt aagaaaccac
     9121 ctacaagagg atggaagagt acttgactca tcacgatgaa taatgtctca ggatcttcta
     9181 gtttaatatg ataagcaggt acgaatgcaa tacctgttaa accaacagcc acagcgccta
     9241 ataaaccaac agccatccag ctaataccta aacgtctagc tttaggtagc atcttgtgtg
     9301 atttaataga cataaacctt acaatgatat gaggttgacc gaaataacct aatccccatg
     9361 aaaatagaga gataattcct ataaatgata accctttaaa taaatttaaa tttgtaggtt
     9421 tcatagctgc tacatcatga aacgttcccc agccgtttaa attcatcata gcaacaatag
     9481 ggaccataac catcgcaatt aacataatga caccttggaa gaaatctgta attgatacag
     9541 ctaaatatcc accaaagaaa gtatagaaaa tgacaatgaa agcaactaat attaaaccga
     9601 aatgataatc taatccaaaa gcactttcaa atagtttacc accagatacg aaaccagaat
     9661 gtgtatataa tgtaaagaat actacgataa tcaatccaga aataatcttt aacacattat
     9721 ttttatcgtt taaacgattt ttaaagaaat ctggtaatgt aattgcatct ccagctaatt
     9781 cggtataaac acgaagtcta ggagcaacaa caaagtaatt tatataagca cctaatgtta
     9841 aaccgattgt aatccacata gctgatagac cagtgctata gacagaacca ggtagcccca
     9901 taatcatcca tccactcata tctgaagctc cagctgataa tgcagtaata tacggtccaa
     9961 tactacgtcc acctaacatg tactcgctta ggttaccagt tgcttgcttg taaccgtaaa
    10021 agccaataac gattagtatc aagaagtaga cggcaatcat aatataagtt tgccaattgg
    10081 catctacttg ttgacttaat gctgtcccca ttgtaagcat gataaacact ccctttattt
    10141 aatttataat ttagttactt gaaaacatta tacataaaga taaatgttta gaaaagtagg
    10201 aaaattaaat ttattctttt tgaaatactt gcataccaat atagaaagcg ctttcgtatt
    10261 ttttggatat gagcaaattt ttataattaa aattgcataa tcataatatt gaattgggtt
    10321 gtctaatatt aaaagagtgt tgttttcata aaaaatgatt tgataaacga atgataatgt
    10381 ttatacagtt tatatgaaat taaagtagca cctcataaat acttagattt ttaattggaa
    10441 atttgataca atttagtgat gaatgactta aaggaggctt ttattaatga caaaagtaac
    10501 acgtgaagaa gttgagcata tcgcgaatct tgcaagactt caaatttctc ctgaagaaac
    10561 ggaagaaatg gccaacacat tagaaagcat tttagatttt gcaaaacaaa atgatagcgc
    10621 tgatacagaa ggcgttgaac ctacatatca cgttttagat ttacaaaacg ttttacgtga
    10681 agataaagca attaaaggta ttccgcaaga attagctttg aaaaatgcca aagaaacaga
    10741 agatggacaa tttaaagtgc ctacaatcat gaatgaggag gacgcgtaag atgagcattc
    10801 gctacgaatc ggttgagaat ttattaactt taataaaaga caaaaaaatc aaaccatctg
    10861 atgttgttaa agatatatat gatgcaattg aagagactga tccaacaatt aagtcttttc
    10921 tagcgctgga taaagaaaat gcaatcaaaa aagcgcaaga attggatgaa ttacaagcaa
    10981 aagatcaaat ggatggcaaa ttatttggta ttccaatggg tataaaagat aacattatta
    11041 caaacggatt agaaacaaca tgtgcaagta aaatgttaga aggttttgtg ccaatttacg
    11101 aatctactgt aatggaaaaa ctacataaag agaatgccgt tttaatcggt aaattaaata
    11161 tggatgagtt tgcaatgggt ggttcaacag aaacatctta tttcaaaaaa acagttaacc
    11221 catttgacca taaagcagta ccaggtggtt catcaggtgg atctgcagca gcagttgcag
    11281 ctggtttagt accatttagc ttaggttcag acacaggtgg ttcaattaga caaccggctg
    11341 catattgtgg cgttgtcggt atgaaaccaa catacggtcg tgtatctcga tttggattag
    11401 ttgcttttgc atcttcatta gaccaaattg gtccattgac tcgaaatgta aaagataatg
    11461 caatcgtatt agaagctatt tctggtgcag atgttaatga ctctacaagt gcaccagttg
    11521 atgatgtaga ctttacatct gaaattggta aagatattaa aggattaaaa gttgcattac
    11581 ctaaagaata cttaggtgaa ggtgtagctg atgacgtaaa agaagcagtt caaaacgctg
    11641 tagaaacttt aaaatcttta ggtgctgtcg ttgaggaagt atcattgcca aatactaaat
    11701 ttggtattcc atcatattac gtgattgcat catcagaagc ttcgtcaaac ctttctcgtt
    11761 ttgacggaat tcgttatggt tatcattcta aagaagctca ttcattagaa gaattatata
    11821 aaatgtcaag atctgaaggt ttcggtaaag aagtaaaacg tcgtattttc ttaggtacat
    11881 ttgcattaag ttcaggttac tacgatgctt actataaaaa atctcaaaaa gttagaacat
    11941 tgattaaaaa tgactttgat aaagtattcg aaaattatga tgtagtagtt ggtccaacag
    12001 cgcctacaac tgcgtttaat ttaggtgaag aaattgatga tccattaaca atgtatgcca
    12061 atgatttatt aacaacacca gtaaacttag ctggattacc tggtatttct gttccttgtg
    12121 gacaatcaaa tggccgacca atcggtttac agttcattgg taaaccattc gatgaaaaaa
    12181 cgttatatcg tgtcgcttat caatatgaaa cacaatacaa tttacatgac gtttatgaaa
    12241 aattataagg agtggaaatc atgcattttg aaacagttat aggacttgaa gttcacgtag
    12301 agttaaaaac ggactcaaaa atgttttctc catcaccagc gcattttgga gcagaaccta
    12361 actcaaatac aaatgttatc gacttagcat atccaggtgt cttaccagtt gttaataagc
    12421 gtgcagtaga ctgggcaatg cgtgctgcaa tggcactaaa tatggaaatc gcaacagaat
    12481 ctaagtttga ccgtaagaac tatttctatc cagataatcc aaaagcatat caaatttctc
    12541 aatttgatca accaattggt gaaaatggat atatcgatat cgaagtcgac ggtgaaacaa
    12601 aacgaatcgg tattactcgt cttcacatgg aagaagatgc tggtaagtca acacataaag
    12661 gtgagtattc attagttgac ttgaaccgtc aaggtacacc gctaattgaa atcgtatctg
    12721 aaccagatat tcgttcacct aaagaagcat atgcatattt agaaaaattg cgttcaatta
    12781 ttcaatacac tggtgtatca gacgttaaga tggaagaggg atctttacgt tgtgatgcta
    12841 acatctcttt acgtccatat ggtcaagaaa aatttggtac taaagccgaa ttgaaaaact
    12901 taaactcatt taactatgta cgtaaaggtt tagaatatga agaaaaacgc caagaagaag
    12961 aattgttaaa tggtggagaa atcggacaag aaacacgtcg atttgatgaa tctacaggta
    13021 aaacaatttt aatgcgtgtt aaagaaggtt ctgatgatta ccgttacttc ccagagcctg
    13081 acattgtacc tttatatatt gatgatgctt ggaaagagcg tgttcgtcag acaattcctg
    13141 aattaccaga tgaacgtaaa gctaagtatg taaatgaatt aggtttacct gcatacgatg
    13201 cacacgtatt aacattgact aaagaaatgt cagatttctt tgaatcaaca attgaacacg
    13261 gtgcagatgt taaattaaca tctaactggt taatgggtgg cgtaaacgaa tatttaaata
    13321 aaaatcaagt agaattatta gatactaaat taacaccaga aaatttagca ggtatgatta
    13381 aacttatcga agacggaaca atgagcagta aaattgcgaa gaaagtcttc ccagagttag
    13441 cagctaaagg tggtaatgct aaacagatta tggaagataa tggcttagtt caaatttctg
    13501 atgaagcaac acttctaaaa tttgtaaatg aagcattaga caataacgaa caatcagttg
    13561 aagattacaa aaatggtaaa ggcaaagcta tgggcttctt agttggtcaa attatgaaag
    13621 cgtctaaagg tcaagctaat ccacaattag taaatcaact attaaaacaa gaattagata
    13681 aaagataatt taaatcatca aactatgaag atttaaaaaa taaacccttg attgctgact
    13741 tagatgcaat cgagggttta tttatatcta tagaagtcat attactttta actttattca
    13801 ttgtacatgt taatggtaaa aatattaatt ttattaatgc gttagcttta attatattaa
    13861 ggcaaactgt ataataaaaa ggtataaaac atttttgtat aaagacaaca ttatatttac
    13921 aacatcattt taaaggtaaa atagcataac tgacgaagtc tatataatga agaacggcaa
    13981 aaaatgctga ataaataaca agctttgtac atattgagat agtatttgtt taagatacaa
    14041 gttggtcttt aacgatatta agaatgatga aataagactg agcctgggtc ataaattcaa
    14101 tgtcctaggc actacaatgt tatattggca gtagttgact gaaagaaaat acgcttgtaa
    14161 caagcttttt tcaattctag tggggcccca acaaagaagc tgactttctg tcagcttaca
    14221 ataatgtgca agttggggta gaacgaaata aattttgtta aatattattt ctgtcccact
    14281 ccctattaga cgaaacaaag atgatgttaa aatatatgaa ttttaagtag agggataaga
    14341 tatgaggaaa cgtgctagaa tcatttataa cccgacatca ggtaaagagc tatttaaaag
    14401 agaattacct gatgccttaa taaaattaga aaaagcggga tatgaaacga gtgcatatgc
    14461 aaccgagaaa ataggtgatg ccacacttga agcagaaaga gctatgcatg aaaattatga
    14521 tgtattaatc gctgcaggtg gtgatggaac attaaatgaa gtagttaatg gtatcgcaga
    14581 aaagcctaat cgtcctaagc taggtgtcat tcctatgggt actgttaatg actttggacg
    14641 tgcattgcat atacctaatg acatcatggg ggcacttgat gtcatcattg aaggtcattc
    14701 tactaaagta gatattggta aaatgaataa tcgatacttt attaatttag ctgcaggcgg
    14761 acaattgacg caagtctctt atgaaacacc gagtaaattg aaatctattg ttggtccatt
    14821 tgcttattac atcaaaggtt tcgaaatgtt acctcaaatg aaagctgtag atttaagaat
    14881 tgaatatgat ggtaatgttt tccaaggaga agcattatta ttctttttag gtttaacaaa
    14941 ttcaatggca ggatttgaaa aattagtgcc ggacgctaag ttagatgacg gctattttac
    15001 gttaattata gttgaaaaat caaaccttgc agaacttggt catattatga ctttagcttc
    15061 aagaggagaa catactaagc atcctaaagt tatttatgaa aaagcaaagg caattaatat
    15121 ttcatcattc acagacttac aactaaatgt agatggagaa tatggtggta aattaccagc
    15181 gaatttctta aatctagaac gtcatataga tgtctttgca cctaatgata ttgtaaatga
    15241 agagttaata aataatgatc atgttgatga caatttaatc gaagaataaa cagaaatagt
    15301 gaaatatata atggcaggtt aggaagcgca taattttgtt cctaacctgt tcattttgga
    15361 ggtaaaattt tgcaagcaat agctaaaaat gacataaaaa cgggaacagt tgttgattta
    15421 acgcatgaag gccatggtgt cgtaaaaata gatcgctttc ctattttcat accacaagct
    15481 ttaattaatg aacaaattga atataaaatt atcaaagtaa aaaagaactt tgcgataggt
    15541 aagttgctta atataaatac gagaagtgag aatcgtgttg caccgccatg tatatattat
    15601 gaacgttgtg gtgggtgcca acttcaacac ttatcatacg aagcacaact tgaaatgaaa
    15661 aaggaacaag tgattaattt gtttcagcga aaagcgcatt ttgataattc taagattaac
    15721 gatacagttg gcatgacaga tccttggaga tatcgaaaca agtcccagat tccagttggt
    15781 aagaatgaac agaatgaagt tattatgggc ttttatcgtc aaagaagcca tgacattatt
    15841 gacatggaaa gttgtttaat acaagattca caacatcaag aagtgatgaa tgaagttaaa
    15901 tctattttaa aagatttaaa tgtaagtatt tatcaagaac aattgaaaaa aggtttaatg
    15961 agacatttgg ttgtaagaac aggatatcat acagacgaaa tgatgattat ttttgtaact
    16021 aatggtaaaa agtggccaca aaaaaatgct gttgttgaga agatattaga tgcatttcct
    16081 aatgtgacaa gcattaaaca aaacattaat gatagccact ctaatgttat tatggggcgt
    16141 caatctataa cgctatatgg taaagataca attattgatc aattaacaga tagtacattt
    16201 aaaataagtg atcagtcatt ctaccaaatt aattctgaac aaacagagaa attatataat
    16261 aaagcaattg aatatgcgca attgacaggt aatgaagttg tattagatac ctattgtggt
    16321 attggaacaa tagggctata tatggcacca catgcaaaac atgtatatgg tgttgaagta
    16381 gtaccttctg caattgaaga cgcacaacaa aatgcgacta taaaccaatg caataataca
    16441 acttttgtct gtgggaaagc tgaagaagtt attttacaat ggaaagcaca agggattaaa
    16501 ccagatgttg taatggttga tccgccaaga aagggctgtg acgaaacgtt tattcagact
    16561 ttgcttacat tagaacctaa gcgtattgtc tatatttcat gtaatcccgc aacacagcaa
    16621 cgtgatgcat tattattagc tgaaaaatat caacttgaag aggtaacacc ggtagatatg
    16681 tttccacaaa caacacatgt tgagacagtg gcattattca atttgaaata gcgcacatga
    16741 tttgagataa aatttcgcct taataagtga agaaagaagt cgtatattaa aattttatta
    16801 cagccaattt actttcttga tatgaacttt ttaaattaaa tgtgacattg tatactatat
    16861 ttaaagaaga ataagaatgt catgatcggg agggttggta atgcataaaa ttgatttaac
    16921 gacaaataat tttcaaatgc gaaggtttat tatcttgcaa cttgttattg cgttattcgt
    16981 gattttattt acgtataagt gggcgttagg tgtcacagcg gttgtcgatc aaaatatcat
    17041 catcaatctt gtatatgggt ttgctggatt cattatttta ttgattttac atgaacttat
    17101 ccatagagca ttatttcttt tatttaaaaa agatagcaaa cctatgttta atataaaaaa
    17161 agacagaatc ttattccaaa cagcagatgc ttgcttcaac aaatggcaat tctcaattat
    17221 tatgctgtca ccactaatcc tattaagtac tggcttgctt attttaatta aggtatttgg
    17281 ttattcatca cttattttta tgtttagtat gcatactgct tattgcttta tagatattct
    17341 gcttgttgca ttaacaatat ctagtagttt taaatatgtt caacaagatg aagatagtat
    17401 ttatttgtat catcaaaagc cggctcaata ataaatttta atatgatgat accaacaaaa
    17461 tgaatttgct tgaattattt cagattacct atgttttctt actcaatcgt cttcatcatc
    17521 atgcttaaat agcaattgat tttataacaa cttgtagtta tctcaatata gttatcgcta
    17581 agtgcaagat ttatgtaaaa aaatcgtaca cagcgtataa ttatgttgag atatttttgt
    17641 gcgttataaa agtaaaaatt agtagggagg tgagcacttg actgagagac gaattattca
    17701 tatagatatg gactattttt ttgcacaagt cgaaatgaga gacaacccca aattgaaagg
    17761 gaaaccagtt attgttggtg gtaaagcaag cagtaggggt gttgtctcta cggcatctta
    17821 tgaagcacga aaatacggcg tccattcagc aatgcctatg tcacaagcgc ataaattatg
    17881 tccaaatgga tactttgtca caagtaattt tggtgcttac cgagaaacat ctgcgcagat
    17941 tatgtctatt ttccgaagtt atacagataa ggtagaaccg atgtcattgg atgaagcgta
    18001 tttagatatt acagaattag tgagaccaga ccttcctgct tcgaaaattg ctcagtatat
    18061 tagaaaagat attctagagc agacacattt aacagcatct gcaggtgttt cttataacaa
    18121 atttttagct aaattagcga gtggtatgaa taaacctgat ggtatgactg tgattgatta
    18181 tcaaaatgtc catgatattt tgatgacatt ggatattgga gattttccag gcgtaggtaa
    18241 agcttccaaa aaagtaatgc atgataatgg tatttttaac ggtagagatt tatatgagaa
    18301 aacggaattt gaattaatac gtttgtttgg aaaaagaggt cggggtttat ataacaaggc
    18361 acgcggtatt gaccatagtg aagtgaaatc atcaagagta agaaaatcag tagggactga
    18421 acgcacattt gcaacagacg tgaatgatga tgaagagatt ttaagaaaag tatgggaatt
    18481 gtcaggtaaa acagctgaac gtctaaataa attacagaag tcagctaaaa ctgtaacggt
    18541 taaaattaaa acttatcaat ttgaaacgct atctaaacag atgagtttaa gagattcggt
    18601 tagttctgaa gaagatattt ataatattgc atatttactt tataacgatt taaaagaccc
    18661 tgatgtacca attcgactta ttggtgtcac tgtaggtaat ttagaacaat caacttataa
    18721 aaatatgacg atatatgact ttatataaaa taaagctccc tgcaaagttt acatttttac
    18781 aatgcttact ttgaagggag tattttattt agtcctagcc tttatctttt agatttttac
    18841 cataaatatt agttacatat gttaaatttt cataattttt cagtagccta aaagtaatca
    18901 ttgcgcatgc tttggcatca ttcaatgcat catgatgacc atgaaaatct aattgataaa
    18961 actccatcat atgttttaaa ccgtatcgat acgaatcaac ggttctttta gcaagttgat
    19021 aactacaaaa gtaagttaaa tttggtgttg gtaaaccaat attttgaatg ctttgatgta
    19081 agacgttcat atcaaatgcc gcgttatgtg cgacaacagg taaatctgca ataaattgca
    19141 tcatatatgg aaatacgtag tcaaacgtag gtgcattttc aacatcttct ggttgtatgc
    19201 catgaacttt aatattttgt tgtgaaaaat agtcttgcgg attcacaaga gtatgaaatg
    19261 tttctgttat ttgactatca atgactttaa ccattccgac agaacaaata cttgtacgtt
    19321 taccatttgc tgtttcaaaa tcaagtgcaa caaacgcatc ttgtatcatg tagtcaatcc
    19381 tttcaattaa ttaaatgtat atattaatag acttttagtc atttgcttac aaacgataca
    19441 taatattata cgtgaaggta taacgtgaaa tctatcattt ggatgtaact ctgattaaaa
    19501 agtaaatgat gatatgcttt aaaatcggtc aaattaattc aacatcaaat ttagagttat
    19561 taacattagt agtttaaaaa ataaatgact aaaaatacaa taattagcct tgaaaatgaa
    19621 aaaacgcaga tcaatgattc agaaaatgaa tctagtgatc tccgtaaagc aaaataatat
    19681 agacattggg ttattgtaag tgacccaaat gcctatcatg tcgtatgata tttatctgtc
    19741 tattgtagtg atgtttaatt attcttcgtc gaatgtacga gcgccaagtt ctttttcgta
    19801 aagatataat gcattgctgt catcgccgat acgagttaaa taattgatgt gagtttcaaa
    19861 cattgattct tcttcgactt gttcatctaa gaaccagttt aagaatgaga tagttgcata
    19921 atctttatct tgacgagcga tttcagataa gttatagaaa cgtcttgtta cttcttgttc
    19981 ttgagataag ctgtctttga aagtttctag tatgcttgaa aagtcaattt ttggtgctga
    20041 aactgctctg aattctgcat gtgcacctct gtcgttaata tagttataaa tcttttgtcc
    20101 atggaaacgt tcttctttag cttgttgaat gaagaagttt gcaaatcctt cgtacgattc
    20161 tttatcacag tatgctgcca ttgccatata tgcgtgtgct gcaaagtact catggttcat
    20221 ttgatcattt aatgcttcta ataaattttt acttaacatt ttgatacacc tcttatttgt
    20281 aaattaaaat tactatgaat taaacttagt ataacaaact cattctcaat tgtaaataat
    20341 a
//
LOCUS       DAMZLE010000035        19393 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMZLE010000035 DAMZLE010000000
VERSION     DAMZLE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.035"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            69..500
                     /gene="mraZ"
                     /locus_tag="POB92_001134"
     CDS             69..500
                     /gene="mraZ"
                     /locus_tag="POB92_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI6578643.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            516..1451
                     /gene="rsmH"
                     /locus_tag="POB92_001135"
     CDS             516..1451
                     /gene="rsmH"
                     /locus_tag="POB92_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI6578644.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            1465..1866
                     /gene="ftsL"
                     /locus_tag="POB92_001136"
     CDS             1465..1866
                     /gene="ftsL"
                     /locus_tag="POB92_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI6578645.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            1847..4081
                     /locus_tag="POB92_001137"
     CDS             1847..4081
                     /locus_tag="POB92_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6578646.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNMSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            4373..5338
                     /locus_tag="POB92_001138"
     CDS             4373..5338
                     /locus_tag="POB92_001138"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI6578647.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVVIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            5340..6689
                     /locus_tag="POB92_001139"
     CDS             5340..6689
                     /locus_tag="POB92_001139"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI6578648.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIIYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            6705..8024
                     /locus_tag="POB92_001140"
     CDS             6705..8024
                     /locus_tag="POB92_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDI6578649.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEQSNDENNDSASSRANNNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAFDNEQPQSAPKEQNGDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            8130..9542
                     /gene="ftsA"
                     /locus_tag="POB92_001141"
     CDS             8130..9542
                     /gene="ftsA"
                     /locus_tag="POB92_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI6578650.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQEIEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDSEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            9575..10747
                     /gene="ftsZ"
                     /locus_tag="POB92_001142"
     CDS             9575..10747
                     /gene="ftsZ"
                     /locus_tag="POB92_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI6578651.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            11007..11798
                     /gene="pgeF"
                     /locus_tag="POB92_001143"
     CDS             11007..11798
                     /gene="pgeF"
                     /locus_tag="POB92_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDI6578652.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            11822..12490
                     /locus_tag="POB92_001144"
     CDS             11822..12490
                     /locus_tag="POB92_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDI6578653.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFLQKKEALPSDAVIHFIGSLQSRKVKGVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDYLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            12487..13050
                     /locus_tag="POB92_001145"
     CDS             12487..13050
                     /locus_tag="POB92_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDI6578654.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            13062..13352
                     /locus_tag="POB92_001146"
     CDS             13062..13352
                     /locus_tag="POB92_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDI6578655.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            13462..14241
                     /locus_tag="POB92_001147"
     CDS             13462..14241
                     /locus_tag="POB92_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDI6578656.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            14265..14882
                     /locus_tag="POB92_001148"
     CDS             14265..14882
                     /locus_tag="POB92_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDI6578657.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTSDNNQQSNDSETTKK"
     gene            15103..17856
                     /gene="ileS"
                     /locus_tag="POB92_001149"
     CDS             15103..17856
                     /gene="ileS"
                     /locus_tag="POB92_001149"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI6578658.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVDGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSITESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            18146..18943
                     /locus_tag="POB92_001150"
     CDS             18146..18943
                     /locus_tag="POB92_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6578659.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         7132 a         2699 c         3745 g         5817 t
ORIGIN      
        1 attttgtata aatagtggtg gatagtgggg agatgtggta aattatatat aaggtgaggt
       61 gataaaaaat gttcatggga gaatacgatc atcaattaga tacaaaagga cgtatgatta
      121 taccgtccaa gtttcgttat gacttaaatg agcgttttat tatcacaaga ggccttgata
      181 aatgtttatt cggttacact ctagacgaat ggcaacagat tgaagagaaa atgaaaacct
      241 tacctatgac aaaaaaagac gcacgtaagt ttatgcgtat gttcttctct ggtgctgttg
      301 aagtagaact tgataagcaa gggcgtatta acatccctca aaacttgagg aaatacgcta
      361 atttaactaa agaatgtaca gtaatcggtg tttcaaatcg tattgagatt tgggatagag
      421 aaacttggaa tgatttctat gaagaatctg aagaaagttt cgaagatatt gctgaagatt
      481 taatagattt tgatttttaa aatggaggaa ttgaagtgtt tcatcatatc agcgttatgt
      541 taaacgaaac cattgattat ttaaatgtaa aagaaaatgg tgtgtacatt gactgtacgc
      601 taggtggagc gggacatgcc ctttatttac taaatcaatt aaatgacgac ggaagattaa
      661 tagcaatcga tcaagaccaa actgcaattg ataatgctaa agaggtatta aaggatcatt
      721 tgcataaggt gacttttgtt catagcaact tccgtgaatt aactcaaata ttaaaagact
      781 taaacattga aaaagtagat ggaatttatt acgacttggg tgtttcaagc ccacaactcg
      841 acattccaga acgaggattc agttatcacc atgacgcaac attagacatg cgtatggacc
      901 aaacacaaga actaacagca tatgaaattg ttaacaattg gtcatatgaa gcgttagtga
      961 agatttttta tcgctatggc gaggagaaat tttcaaaaca gatagctcga agaatcgaag
     1021 cacatcgcga acaacaacca ataacaacaa cattagaatt agttgacatt ataaaagaag
     1081 gtattcctgc aaaagcaaga agaaaaggcg gacatcctgc aaaacgagta tttcaagcac
     1141 tacgaattgc agtaaacgat gaattgtcag cttttgaaga ttcaatagaa caagcgattg
     1201 aattagtgaa agtagatggc aggatttcgg taatcacttt ccattcttta gaagatcgtt
     1261 tatgtaaaca ggtgttccaa gaatatgaaa aaggtccaga ggtaccaaga ggattaccag
     1321 ttataccaga agcatataca cctaagttaa agcgtgttaa tcgtaaaccg attaccgcta
     1381 cagaagaaga tttagatgac aataacagag cacgaagcgc gaaattacgt gtagctgaaa
     1441 tacttaaata aggagcaatt tataatggct gtagaaaaag tgtaccaacc atatgacgaa
     1501 caagtttata atagtatacc gaagcaacaa ccacaaacta agcccgaaaa gaagactgtt
     1561 tcgagaaaag tggttgtaca attaactaaa tttgaaaaag ttttatacat aactttgatt
     1621 actgtaattg ctatgttaag tatttatatg ctatctttaa aaatggatgc gtatgatacg
     1681 cgaggaaaga ttgcagattt agattataaa atagataaac aatcaagtga aaacagtgct
     1741 ttacaatctg aaatcaaaaa gaattcttct tatgaacgca tatacgaaaa ggctaagaaa
     1801 caggggatga gccttgagaa cgataatgta aaggtagtgc gtagtaatgg cgaagcaaaa
     1861 aattaaaatt aaaaaaaata aaataggggc agtcctactt gttggtttat tcggactgct
     1921 cttttttata ttggttttaa gaatttcata tatcatgatt actggacatt ctaatggtca
     1981 agatttagtc atgaaggcaa atgaaaagta tttagttaag aatgcacaac aaccagaacg
     2041 aggaaagata tatgatcgta atggtaaagt gctagcagaa gatgtagaaa gatataaact
     2101 tgttgcagta atagataaaa aggcgagtgc caattctaaa aaacctaggc atgtagttga
     2161 taaaaaagag actgcaaaga aattatctac agtcattaat atgaagccag aggaaattga
     2221 aaagagactt agtcaaaaga aagctttcca aattgaattt ggacgcaaag gaacaaattt
     2281 aacgtatcag gacaaattga aaatagagaa aatgaatttg cctggtattt ctttattgcc
     2341 tgaaacagaa cgcttttatc caaatggcaa ttttgcatca cacttaattg gtagagctca
     2401 gaaaaatccg gatactggtg aacttaaagg tgcacttgga gttgaaaaga tttttgatag
     2461 ttatttaagt ggatctaaag gatcattgag atatattcat gatatttggg gatatatcgc
     2521 accaaatact aaaaaagaga agcagcctaa acgtggtgat gatgtccatt taacaatcga
     2581 ttcaaatatt caagtatttg ttgaagaagc tttagatggc atggttgaaa gataccagcc
     2641 gaaagattta tttgcggttg tcatggatgc caaaactgga gaaattttag catacagtca
     2701 gcgaccaaca tttaatcctg aaactggtaa agactttggt aaaaagtggg caaatgacct
     2761 ttatcaaaac acatacgagc ctggatcaac atttaaatca tatgggttag cagctgctat
     2821 tcaagaaggt gcttttgatc ctgataagaa atataaatct ggacatagag atattatggg
     2881 ttcacgtatt tcagactgga atagagtcgg ttggggtgaa atcccaatgt cactcggatt
     2941 tacttattca tctaatacat tgatgatgca tttacaagat ttagttggtg cagacaaaat
     3001 gaaatcttgg tatgaacgat ttggatttgg aaaatcaact aaaggtatgt ttgatggaga
     3061 agcacctggt caaattggat ggagtaatga gttgcaacaa aaaacgtcat catttggtca
     3121 atcgacaaca gtaacacctg ttcaaatgtt acaagcgcaa tcagcgttct ttaatgatgg
     3181 taatatgtta aaaccatggt ttgtgaatag cgttgaaaat cctgttagta aaagacaatt
     3241 ttataaaggg caaaaacaaa tcgcaggcaa accaataaca aaagatactg ctgaaaaagt
     3301 tgaaaagcaa ttggatttag ttgtgaatag taagaagagt cacgctgcaa actatcgtat
     3361 tgatggttat gaggtcgaag gtaagactgg tacagcacaa gtcgctgcac ctaatggtgg
     3421 tggatacgtt aaaggtccaa acccatattt tgtaagtttt atgggtgacg cgccgaagaa
     3481 aaatcctaaa gttattgtat acgctggtat gagcttggca caaaaaaatg accaagaagc
     3541 ttatgaatta ggtgttagta aagcgtttaa accaataatg gaaaatactt tgaaatattt
     3601 aaatgtaggt aaatcaaaag atgacacatc taatgcagag tatagtaaag tgccagatgt
     3661 tgaaggtcaa gacaaacaaa aagctattga taatatgagt gcaaaatcat tagaaccagt
     3721 tactatcggt tctggcacac aaataaaagc acaatctata aaagcaggga ataaagtctt
     3781 acctcatagt aaagtactgt tattaacaga cggagactta actatgcctg acatgtcagg
     3841 atggacgaaa gaagatgtca ttgcttttga aaacctaaca aatattaaag taaatttaaa
     3901 aggtagcggt tttgtgtccc accaatcaat tagtaaggga caaaaactta ctgaaaaaga
     3961 taaaatagac gtagaatttt catcagagaa tgtagacagc aattcgacga ataattctga
     4021 ttcaaattca gatgataaga agaaatctga cagtaaaact gacaaggata agtcggacta
     4081 acaagaatgg ctacattaaa taagggtgta ttgtcactat ttgtttttac taaagtaatg
     4141 ctgtgataga aaacaacatc ttcatttaat ctttataaat aagtgttgat agagaatttt
     4201 aaggtagacg aggtcagatt aacttgtttg ttataggcaa actttttata ctggcctctt
     4261 atgccgtaaa aaagaaaaat tataaatttt gattacaact ttgtttattt aattgaaaaa
     4321 ttgataatat attagtggtt aaaaaagtct ggattaagaa gaggagatag ttatgatttt
     4381 tgtatatgcg ttattagcgc tagtgattac atttgttttg gtacctgttt taatacctac
     4441 attaaaaagg atgaaatttg gtcaaagtat tcgagaagaa ggtccacaaa gccatatgaa
     4501 gaagactggt acaccaacga tgggtggact aacatttcta ttaagtattg tgataacgtc
     4561 tttggtggtt attatatttg tggatcaagc taatccaatc atactgttat tatttgtgac
     4621 gattggtttt gggttaattg gttttataga tgattatatt attgttgtta aaaagaataa
     4681 ccaaggttta acaagtaaac agaagttttt ggcgcaaatt ggtattgcga ttattttctt
     4741 tgttttaagt aatgtgtttc atttggtgaa tttttctacg agcatacata ttccatttac
     4801 gaatgtagca atcccactat catttgcata tgttattttc attgtttttt ggcaagtagg
     4861 tttttctaat gcggtaaatt taacagatgg tttagatgga ttagcaactg gactgtcaat
     4921 tatcggattt acaatgtatg ccatcatgag ctttgtgtta ggagaaacgg caattggtat
     4981 tttctgtatc attatgttgt ttgcactttt aggattttta ccatataaca ttaaccctgc
     5041 taaagtgttt atgggagata caggtagctt agctttaggt ggtatatttg ctacgatttc
     5101 aatcatgctt aatcaggaat tatcattaat ttttataggt ttagtattcg taattgaaac
     5161 cttatctgtt atgttacaag tcgctagctt taaattgact ggaaagcgta tatttaaaat
     5221 gagtccgatt catcatcatt ttgaattgat aggatggagc gaatggaaag tagttacagt
     5281 attttgggct gttggtctga tttcaggttt aatcggttta tggattggag tgcattaaga
     5341 tgcttaatta tacagggtta gaaaataaaa atgtattagt tgtcggtttg gcaaaaagtg
     5401 gttatgaagc agctaaatta ttaagtaaat taggtgcgaa tgtaactgtc aatgatggaa
     5461 aagacttatc acaagatgct catgcaaaag atttagaatc tatgggcatt tctgttgtaa
     5521 gtggaagtca tccattaacg ttgcttgata ataatccaat aattgttaaa aatcctggaa
     5581 taccttatac agtatctatt attgatgaag cagtgaaacg aggtttgaaa attttaacag
     5641 aagttgagtt aagttatcta atctctgaag caccaatcat agctgtaacg ggtacaaatg
     5701 gtaaaacgac agttacttct ctaattggag atatgtttaa aaaaagtcgc ttaactggaa
     5761 gattatccgg caatattggt tatgttgcat ctaaagtagc acaagaagta aagcctacag
     5821 attatttagt tacagagttg tcgtcattcc agttacttgg aatcgaaaag tataaaccac
     5881 acattgctat aattactaac atttattcgg cgcatctaga ttaccatgaa aatttagaaa
     5941 actatcaaaa tgctaaaaag caaatatata aaaatcaaac ggaagaagat tatttgattt
     6001 gtaattatca tcaaagacaa gtgatagagt cggaagaatt aaaagctaag acattgtatt
     6061 tctcaactca acaagaagtt gatggtattt atattaaaga tggttttatc atttataaag
     6121 gtgttcgtat tattaacact gaagatctag tattgcctgg tgaacataat ttagaaaata
     6181 tattagcagc tgtgcttgct tgtattttag ctggtgtacc tattaaagca attattgata
     6241 gtttaactac attttcagga atagagcata gattgcaata tgttggtact aatagaacta
     6301 ataaatatta taatgattcc aaagcaacaa acacgctagc aacacagttt gccttaaatt
     6361 catttaatca accaatcatt tggttatgtg gtggtttgga tcgagggaat gaatttgacg
     6421 aactcattcc ttatatggaa aatgttcgcg cgatggttgt attcggacaa acgaaagcta
     6481 agtttgctaa actaggtaat agtcaaggga aatcggtcat tgaagcgaac aatgtcgaag
     6541 acgctgttga taaagtacaa gatattatag aaccaaatga tgttgtatta ttgtcacctg
     6601 cttgtgcgag ttgggatcaa tatagtactt ttgaagagcg tggagagaaa tttattgaaa
     6661 gattccgtgc ccatttacca tcttattaaa gggtgtgagt attgatggat gataaaacga
     6721 agaacgatca acaagaatca aatgaagata aagatgaatt agaattattt acgaggaata
     6781 catctaagaa aagacggcaa agaaaaagat caaaggctac acatttttct aatcaaaata
     6841 aagatgatac atctcaacaa gctgattttg atgaagaaat ttacttgata aataaagact
     6901 tcaaaaaaga acaaagcaat gatgaaaata atgattctgc ttctagtcgt gcaaataata
     6961 ataatatcga tgattctaca gactctaata ttgaaaatga ggattataga tataatcaag
     7021 aaattgacga ccaaaatgaa tcgaatggaa ttgcattcga caacgaacaa cctcaatcag
     7081 ctcctaaaga acaaaatggc gactcgaatg atgaggaaac agtaacgaaa aaagaacgaa
     7141 aaagtaaagt aacacaatta aagccattaa cacttgaaga aaagcggaag ttaagacgta
     7201 agcgacaaaa acgaatccaa tacagtgtta ttacaatatt ggtattgttg attgctgtta
     7261 tattaattta catgttttca ccacttagta aaattgcgca tgtaaatata aatggaaata
     7321 atcacgttag tacttcaaag ataaacaaag ttttaggtgt taaaaatgat tcaaggatgt
     7381 atacgtttag taaaaaaaat gctattaatg atctcgaaga gaatccatta atcaaaagtg
     7441 ttgagataca caagcaatta ccaaacacat taaacgtaga tatcacagaa aatgaaatta
     7501 ttgctttagt gaaatataaa ggtaaatatt tacctttatt agaaaatggt aaattgctta
     7561 aaggttcaaa tgatgtcaaa attaatgatg cacctgtcat ggatggtttc aaaggtacaa
     7621 aagaagatga tatgattaag gcgttatctg aaatgacacc tgaagttaga cgatatattg
     7681 ccgaagtgac atacgcccca agtaaaaaca aacaaagcag aattgaattg tttacgacag
     7741 atggacttca agtaatcggt gatatttcga cgatatctaa gaaaatgaaa tattatccgc
     7801 agatgtcaca atcattatca agggatagtt cgggtaaact aaaaacacga ggctatattg
     7861 atttatcagt cggtgcttca tttatcccat accgtggaaa cacgtctagt caatcagaaa
     7921 gcgataaaaa tgtgactaaa tcatctcaag aggaaaatca agcaaaagaa gaattacaaa
     7981 gcgttttaaa caaaattaac aaacaatcaa gtaagaataa ttaaaaaatt ttgatattgt
     8041 ctatgtttat agttcacaag ccattcaacg tattgtaaac taaggatagt gtattttttt
     8101 aatagtaatt tgtcaggagg tgcctatcta tggaagaaca ttactacgta agtattgata
     8161 ttggatcatc aagcgtaaaa acaatagtag gcgagaaatt tcacaatggt ataaatgtga
     8221 taggtacagg acaaacctac acgagcggta taaaaaatgg tttaattgat gattttgata
     8281 ttgcgcgaca agcaatcaaa gacacaatta aaaaggcatc aatcgcttcg ggtgttgata
     8341 ttaaagaagt tttcctgaaa ttacctatca ttggaacgga agtttatgat gaatcaaatg
     8401 aaatcgactt ttatgaggat acagaaatca acggttcaca tatcgaaaaa gtattagaag
     8461 gtattagaga aaaaaatgat gtgcaagaaa tagaagtaat taatgtgttc ccgattcgtt
     8521 ttatagtcga taaagaaaat gaggtttcag accctaaaga attaattgcc agacattcat
     8581 taaaggttga agcaggcgta attgctattc aaaaatcgat tttaattaat atgattaaat
     8641 gcgtagaagc atgtggtgtt gatgtattag atgtttactc tgatgcatat aactatggtt
     8701 caatcctaac agctactgaa aaagagttag gtgcatgtgt cattgatatt ggtgaagacg
     8761 ttacgcaagt tgctttttat gaacgcggtg aattagtaga tgctgattct atcgaaatgg
     8821 cagggcgtga tattacagac gatattgcac aaggattaaa cacttcttat gaaactgctg
     8881 aaaaagttaa acaccaatat ggtcatgcat tctatgattc tgcttcagat caagatatct
     8941 tcactgttga acaggttgat agtgatgaaa cagtacagta tactcaaaaa gatttgagtg
     9001 actttattga agcgcgtgta gaagaaatat tcttcgaagt atttgatgtt ttacaagatt
     9061 taggattaac aaaagtaaat ggtgggttta ttgtaactgg tggatctgca aacttacttg
     9121 gcgtaaaaga attattatca gatatggtaa gtgaaaaagt tagaattcac acgccatcac
     9181 aaatgggaat tagaaaacct gaattttctt cagcaatttc tacaatttct agtagtatcg
     9241 cttttgatga gttattagat tatgttacaa ttaattatca tgatagtgaa gaaactgaag
     9301 aagatgttat tgatgtgaaa gacaaagata acgaatctaa attaggcgga tttgattggt
     9361 ttaaacgtaa aacaaacaaa aaagatactc atgaaaatga agtagagtca acagatgaag
     9421 aaatttatca atcagaagat aatcatcagg aacataaaca gaatcatgaa catgttcaag
     9481 acaaagataa agataaagaa gaaagtaaat tcaaaaaact aatgaaatct ctatttgaat
     9541 gattattggc caataaaact aggaggaaat ttaaatgtta gaatttgaac aaggatttaa
     9601 tcatttagcg actttaaagg tcattggtgt aggtggtggc ggtaacaacg ccgtaaaccg
     9661 aatgattgac cacggaatga ataatgttga atttatcgct atcaacacag acggtcaagc
     9721 tttaaactta tctaaagctg aatctaaaat ccaaatcggt gaaaaattaa cacgtggttt
     9781 aggagcagga gctaatcctg aaatcggtaa aaaagctgca gaggaatctc gtgaacaaat
     9841 tgaagatgca atccaaggtg cagacatggt atttgttact tctggtatgg gtggcggaac
     9901 tggtactggt gcagcaccag tcgttgctaa aattgcaaaa gaaatgggcg cattaactgt
     9961 tggtgttgta actcgtccat ttagttttga aggacgtaaa cgtcaaactc aagctgctgc
    10021 tggagtagaa gctatgaaag ctgcagtaga tacattaatc gttataccaa atgaccgttt
    10081 attagatatc gttgacaaat ctacgccaat gatggaagca tttaaagaag ctgataacgt
    10141 gttacgccaa ggtgtacaag gtatctcaga cttaatcgct gtttctggtg aagtaaactt
    10201 agactttgca gacgttaaga caattatgtc taaccaaggt tctgcattaa tgggtattgg
    10261 tgtttcttct ggtgaaaata gagcggtaga agctgctaaa aaagcaatct cttctccatt
    10321 acttgaaaca tctatcgttg gtgcacaagg tgtgcttatg aatattactg gtggcgagtc
    10381 attgtcatta tttgaagcac aagaggctgc tgatattgtc caagatgctg cagatgaaga
    10441 cgttaatatg attttcggta cagttattaa tcctgaatta caagatgaga ttgttgtaac
    10501 agttattgca actggttttg atgacaaacc aacatcacat ggtcgtaaat ctggtagcac
    10561 tggattcgga acaagcgtaa atacttctag caatgcaact tctaaagatg aatcattcac
    10621 ttcaaattca tcaaatgcac aagcaactga tagtgtaagt gaaagaacac atacaactaa
    10681 agaagatgat attcctagct tcattagaaa tagagaagaa agacgttcaa gaagaacaag
    10741 acgttaatcg gttaatatat atacacaaat aattcaacac aaatcatcag ataacatatc
    10801 tgatgatttt tttactaatt tttagaacat gtagaaggac atttaagttt ttcaaagtta
    10861 ttaaaagtgt ttaagtatcg tgtcaaaatt aagtcaaaaa ttatttgcgc aacattttaa
    10921 ctttaaacat aaatgttata ttatataatt attaactttg tacagttaga cgaagataat
    10981 ttaaatgaaa tgatggtgac gatcgagtga atgataattt taaaaagcaa ccgcatcatt
    11041 taatatatga agagttatta caacaaggta ttactctagg tattacaact agaggcgatg
    11101 gtttaagtga ctatcctaaa aatgctttta atatggcgag atatattgat gatcgcccat
    11161 ataatattac tcaacatcaa ttgcaattag ctgaagaaat tgcgtttgat agaaaaaatt
    11221 gggtgtttcc cattcaaaca catgaaaata aagtcgcttg tattacaaag gatgatatag
    11281 gcacaaatat agacacttta actgatgcgc ttcatggtat tgatgcgatg tacacatatg
    11341 atagtaatgt cttattaacg atgtgttatg cagactgtgt accagtatat ttttatagta
    11401 caaaacatca ttttattgca ttggcgcatg caggttggcg tggtacctat actgaaattg
    11461 taaaagaagt gctaaaacat gtgaactttg atttgaaaga cttacatgtc gttattggac
    11521 catctacatc atcaagttat gaaattaatg atgatattaa aaataaattt gaaacattgc
    11581 caattgatag tgccaactat attgaaacta gaggacgaga tcgtcatggt attgatttga
    11641 aaaaagccaa tgctgcatta ttaaattatt atggtgttcc taaagaaaat atttatacga
    11701 cagcgtatgc tacatctgaa catttagaat tatttttctc ttatcgatta gaaaaaggtc
    11761 aaacaggacg catgttagca ttcattggtc aacagtaaac aaggaggaga tatgcttgcg
    11821 tgtgaaagat aatttacaac aaatctcaac acaaattaat gacaaaagtg aaaaaaataa
    11881 tttttcaaca aaaccaaacg tgattgcagt tacaaaatat gttacaatag agcgagctaa
    11941 agaagcgtat gaggctggaa taagacattt tggtgagaat agattggaag gctttttaca
    12001 aaagaaagaa gcattaccat cagatgcggt gatccatttt ataggatcat tacaatctcg
    12061 aaaagttaag ggcgttataa acgacgtaga ttatttccat gctttagatc gattgagctt
    12121 agccaaagaa attaacaaac gtgcagaaca taaaattaaa tgtttcttgc aagtgaacgt
    12181 ttcgggagaa gcttctaaac atggtattgc tttagaagat gttgatcagt ttatagatta
    12241 tcttaaaaaa tatgacaaaa tcgaaattgt aggtttaatg acgatggcac cattgacaga
    12301 tgatgaagca tatattagat cgttatttaa acagttacgt ttgaaaaaag aagaaataca
    12361 acgactcaat ttagaatatg cgccttgtga tgaattatca atgggaatga gtaatgacta
    12421 tcttattgca gttgaagaag gtgcgacgtt tgttagaatt gggactaaac ttgtaggaga
    12481 agaggagtga gccacttggc tttaaaagat ttatttagtg gattttttgt aatagatgat
    12541 gaagaggaag tagaagtacc tgacaaacaa caacaggtca atgaagcgcc agcaaaagag
    12601 cagtcacaac aaacaacaaa acaaaacgca atcaaatcag tccctcaaaa atctgcatca
    12661 agatatacaa caacgtcaga agaaaggaat aaccgtatgt ctaattattc aaaaaataat
    12721 tcacgtaatg ttgtaactat gaacaatgct acaccaaaca atgcatcaca agaaagttca
    12781 aaaatgtgtt tattcgaacc acgtgttttt tcagatacac aagatattgc tgatgagctt
    12841 aaaaaccgcc gtgcgacact tgtcaattta caacgtattg ataaagtatc agcgaaaaga
    12901 attattgatt ttttaagcgg tactgtttat gcaatcggtg gagatatcca acgtgtaggt
    12961 actgatattt tcttatgtac gcctgataat gtggaagtag ctggaagcat tacagaccat
    13021 attgaaaata tggaacattc attcgactaa ggagttaaca tatggatata aatgtgctag
    13081 ctacaatatt taaatttatc ctttttgttg ttgaaattta ttatttcggc atgattatat
    13141 atttctttac atcttgggta ccaagtatta gagaaactaa ggtaggttat tttttagcga
    13201 aaatatatga acctttctta caaccattta gaaaagtaat tccacctatt ggaattatcg
    13261 acatatcatc aatcgctgca attatcgttt tagtattatt ccaaaaaggg ttactccaaa
    13321 tctttaattg gattttaatt caattacaat aaattaagcg acataacgag gcaagggtac
    13381 atgatacttt agcctcgttt ttgatatgta tttttctgaa tataagggca atagatggta
    13441 ttttataatt tttttaaggt agtgattaac atagatattt atcaacactt tagacaggaa
    13501 gaatacgaat taattgatca gctaacggat aaatgtgatc aagcggaaca gcattatgca
    13561 ccagtattaa cgcatttttt agatccaaga gggcaatata tattggaagt gatttgtggc
    13621 agttatgaag atttaaacgt atctttttat ggtggaccta atgctgaaag aaaaagagca
    13681 atcatttcgc cgaactatta tgaacctaaa gaaagcgact ttgaattaac tttaatggaa
    13741 atagattatc ctgaaaaatt cgtcacttta aaacatcaac atattttagg gacattaatg
    13801 tctttaggta tcgaacgcga acaagttgga gatataattg tgaatgaacg aattcaattt
    13861 gttttgacaa gtagattgga atcatttatt atgttagaat tacaacgtat taaaggcgca
    13921 tcagttaaac tttatactat tccagtaaca gatatgatac aatctaatga gaattggaaa
    13981 aatgaaagtg caacagttag ttctttaagg ttagatgttg ttattaaaga aatgatacgt
    14041 aaatcacgta cgattgcgaa acaactaatc gaaaaaaaac gtgttaaagt gaatcacact
    14101 attgttgatt cagcagattt tcaattacaa gcaaatgatt taatatccat ccaaggtttt
    14161 ggtagagcac acattactga cttaggtggt aaaactaaaa aagataaaac gcacattacc
    14221 tatagaacat tattcaaata gtaatgattt aaggaggata acaaatgcct tttacaccaa
    14281 atgaaattaa gaataaagag ttttcacgtg taaagaatgg tttagaacct actgaagttg
    14341 ctaatttttt ggagcaacta agcactgaaa ttgaacgtct taaagaagat aaaaaacaac
    14401 ttgaaaaagt aatcgaagag agagatacta atattaagtc ttatcaagac gtgcatcaat
    14461 ctgtaagtga tgctttgata caagctcaaa aagctggtga agaaactaag caagctgcag
    14521 aaaaacaagc tgaagcgatt atagctaagg cagaagcgca agctaatcaa atggttggtg
    14581 acgcggtaga aaaagcacgc cgtttagcat tccagactga agatatgaaa cgtcaatcaa
    14641 aagtatttag atcgcgtttc cgtatgttag ttgaagcgca attagactta ttaaaaaacg
    14701 aagattggga ttacttgttg aattatgatt tagacgctga acaagtgacg cttgaaaata
    14761 ttcatcattt gcatgaaaat gatttaaagc cagatgaagt tgcagcaaat gcacaaaata
    14821 atgcatcaaa tacatcagac aataatcaac aatccaatga ttcagaaaca actaagaagt
    14881 aagaattaaa taaagacaga cgcgtaatat acatttaact tttcacagcg aattaggtaa
    14941 tggtgagagc ctagtaaaag catgtatgtt atatcactgg ctttttaata tttaaataat
    15001 gtaatgagag aactctaagt tgagttaata agggtggtac cgcgagcaat cgtccctttt
    15061 aatttaactt agagtttttt aaatttttaa ggagtgaaaa aaatggatta caaagaaacg
    15121 ttattaatgc ctaaaacaga tttcccaatg cgaggtggtt taccaaacaa ggaaccgcaa
    15181 attcaagaaa aatgggatgc agaagatcaa taccataaag cgttagaaaa aaataaaggt
    15241 aacgaaacat tcattttaca tgatggccca ccatacgcga atggtaactt acatatggga
    15301 catgccttga acaaaatttt aaaagacttt attgtacgtt ataaaactat gcaagggttc
    15361 tatgcaccat acgtaccagg ttgggataca catggtttac caattgaaca agcattaacg
    15421 aaaaaaggtg ttgaccgaaa gaaaatgtca acagctgaat tccgtgagaa atgtaaagaa
    15481 tttgctttag aacaaattga attacagaaa aaagatttta gacgtttagg tgttcgtggt
    15541 gactttaatg atccatatat tacattaaaa cctgaatacg aagctgcaca aattcgtatt
    15601 tttggagaaa tggcagataa aggtttaatt tataaaggta aaaagccagt ttattggtct
    15661 ccttcaagtg agtcttcatt agcagaagca gaaattgaat atcacgataa acgttcagca
    15721 tcaatttacg ttgcatttga cgttaaagat gacaaaggtg tcgtagatgc agatgctaaa
    15781 tttattatct ggacaacaac gccatggaca attccatcaa atgttgcgat taccgttcat
    15841 cctgaattaa aatatggtca atacaatgta gatggcgaaa aatatattat tgcagaagcc
    15901 ttatctgacg ctgtagcaga agcactggat tgggataaag catcaatcaa attagaaaaa
    15961 gaatacacag gtaaagaatt agagtatgtt gtagcacaac atccattctt agacagagaa
    16021 tcgttagtga ttaatggtga tcatgttact acagatgctg gtacaggttg tgtacataca
    16081 gcaccaggtc acggggaaga tgactatatt gttggtcaaa aatatgaatt gccagtaatt
    16141 agtccaatcg atgataaagg tgtatttact gaagaaggcg gccaatttga aggaatgttc
    16201 tatgataaag ctaataaagc cgttactgat ttattaacag aaaaaggtgc actattaaaa
    16261 ttagacttta ttacacatag ctatccacac gactggagaa caaaaaaacc tgtaatcttc
    16321 cgtgctacac cacaatggtt tgcctcaatc agtaaagtaa gacaagatat tttagatgca
    16381 atcgaaaata caaacttcaa agtaaattgg ggtaaaacac gtatttacaa tatggttcgt
    16441 gaccgtggcg aatgggttat ttctcgtcaa cgtgtgtggg gtgtaccgtt accagtattt
    16501 tatgctgaaa atggcgaaat tatcatgacc aaagaaacag tgaatcatgt tgctgattta
    16561 tttgcagaac acggttcaaa tatttggttt gaaagagaag cgaaagactt actaccagaa
    16621 ggatttacac atccaggcag ccctaacggt acatttacta aagaaacaga cattatggac
    16681 gtttggtttg attctggttc atcacaccgt ggcgtgttgg aaacaagacc ggaattaagt
    16741 ttcccagcgg atatgtattt agaaggtagt gaccaatatc gtggttggtt caactcttct
    16801 atcacaactt cagttgctac aagaggagta tcaccttata aattcttact ttctcatggt
    16861 tttgttatgg acggtgaagg taagaaaatg agtaaatctt taggtaatgt gattgtacct
    16921 gaccaagtgg ttaaacaaaa aggtgctgat attgcgagac tttgggtaag tagtacggac
    16981 tatttagctg atgttagaat ttctgatgaa attttaaaac aaacatctga tgtttatcgt
    17041 aaaatcagaa atacattaag atttatgtta ggtaacatta acgatttcaa tcctgacaca
    17101 gatagcatta ctgaatcaga gttattagaa gtggatcgtt acttgctaaa tcgtttacgt
    17161 gaatttactg caagtacgat taacaactat gaaaactttg actacttaaa tatttatcaa
    17221 gaagttcaaa actttatcaa tgttgagtta agtaatttct atttggatta cggtaaagat
    17281 attttatata ttgaacaacg agattctcat atccgtcgta gtatgcaaac agtgttatat
    17341 caaattttag ttgatatgac gaagttgtta gcaccaatct tagtgcatac agctgaagaa
    17401 gtttggtctc atacaccaca tgttaaagaa gaaagtgttc acttagcaga catgcctaaa
    17461 gttgtagaag tagatcaagc tttattggat aaatggcgta catttatgaa tttacgtgat
    17521 gatgtgaacc gtgcattaga aactgctcgt aatgaaaaag ttattggtaa atcattagaa
    17581 gctaaagtta cgattgctag taacgataaa tttaatgcat ctgaattctt aacttcattt
    17641 gatgcattac atcaattatt tatcgtgtca caagttaaag ttgtagataa gttagatgat
    17701 caggcaacag cttatgaaca tggtgatatt gtcatcgaac atgcagatgg tgaaaaatgt
    17761 gaaagatgtt ggaactattc agaggatctt ggtgctgttg atgaattgac gcatttatgc
    17821 ccacgatgcc aacaagttgt aaaatcactt gtataattga aattgtataa agtactcata
    17881 cagatgatat aaattaaagc tctcttcata atcatgttgt agtttttgtt gacatgatga
    17941 agagagtttt tttgtgaata aaaaaatgac caagttaccg gtcatatatg taaaaaatgt
    18001 gcgatttact aaaataaaaa ttattcagga atggtacaaa ttctctgagg catataaatg
    18061 cgttatagtt gctattctca attatgttcg cgataatttt aagtaaaagt aagcacagat
    18121 attgaatttg ataggagtta attgaatgta tcataacagt aacgcaaact ttgtcaatgg
    18181 tatcacttta aatgtgagag ataagaatga attaaagcca ttttatgagg acatattagg
    18241 attaaatatt ataaatgaga cattaacatc gatacaatat gaagtaggtc aaaataatca
    18301 tgtcattaca cttgttgaat tacaaaatgg acgtgaacct ttaatgtccg aagcgggact
    18361 gtttcatatc gcaattaaac tacctcaaat tagtgattta gctaatttac taattcattt
    18421 aagcgaatat gatattccag ttaacggagg tatacagcct gcttcgttat cattattttt
    18481 tgaagacccg gaaggaaacg gttttaaatt ttatgttgat aaagacgaag cgcaatggac
    18541 gaggcaaaat gatttagtaa aaattgatat tagaccatta aatgtaccga gattagtgag
    18601 tcatgcaaca aaattgttat ggttaggtat tccagatgac gctattatag gtgcattgca
    18661 tattaagaca attcatttat cagaggtaaa agagtactac ctcgattatt ttggattaga
    18721 gcaatcggca tatatggatg attattcaat atttttagca tcgaatggct attatcaaca
    18781 tttggccatg aatgattggg tatcagcaac gaaacgtgta gaaaattttg atacgtatgg
    18841 attagcaatt gttgactttc attatcctga aacaacacat ttaaatttac aaggtccgga
    18901 tggtatctat tatcgcttta atcatatcga agttgaagat tagtatatac tttgaatgga
    18961 cgaaccatat aatgaatcgt ttttaatgat ctttttatac aagctatgaa ggaggctggg
    19021 acataaatcc ctaaaaaaca gcagtaagat aattttcaat tagaaaatat cttactgctg
    19081 ttctctattt atacaatact tcgtattgaa tggcttcgct ttgcccatct ggcacattac
    19141 tgtaaaattc tataaataga atttttgatg atgggtccct tcctagggtg ccgtctcagc
    19201 taccccaacc ggcacattgt tgtaagctga ctatatgtca gcttctgtgt tggggcccct
    19261 gtcttcgact ggcactgctc cctcaggagt ctcgccatta atactacgta ttaacatgta
    19321 attttacttt gaaatacttt aaagcccaaa ttaatcaaaa gctttctgaa ccagaaacga
    19381 aaaaatctat agt
//
LOCUS       DAMZLE010000036        19121 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMZLE010000036 DAMZLE010000000
VERSION     DAMZLE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.036"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..291)
                     /locus_tag="POB92_001151"
     CDS             complement(<1..291)
                     /locus_tag="POB92_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDI6578660.1"
                     /translation="MDKSLEINLKELEGIMYKEKVIDVKEYDNLKVTFETLNDAPKSE
                     IPTPFTIEQMLYKLETKNLYFPEIKSKEKCVELLHSVGYYNLKHFMYNEFGKD"
     gene            complement(803..2344)
                     /gene="guaA"
                     /locus_tag="POB92_001152"
     CDS             complement(803..2344)
                     /gene="guaA"
                     /locus_tag="POB92_001152"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI6578661.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCECKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(2369..3835)
                     /gene="guaB"
                     /locus_tag="POB92_001153"
     CDS             complement(2369..3835)
                     /gene="guaB"
                     /locus_tag="POB92_001153"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI6578662.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(3873..5141)
                     /locus_tag="POB92_001154"
     CDS             complement(3873..5141)
                     /locus_tag="POB92_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDI6578663.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(5141..5719)
                     /gene="xpt"
                     /locus_tag="POB92_001155"
     CDS             complement(5141..5719)
                     /gene="xpt"
                     /locus_tag="POB92_001155"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6578664.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(5950..6052)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            6232..6639
                     /locus_tag="POB92_001156"
     CDS             6232..6639
                     /locus_tag="POB92_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6578665.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            6782..7444
                     /locus_tag="POB92_001157"
     CDS             6782..7444
                     /locus_tag="POB92_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578666.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            7562..8518
                     /locus_tag="POB92_001158"
     CDS             7562..8518
                     /locus_tag="POB92_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578667.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKADNAGDGTTT
                     KVDKEDQISATEAIEKASVEQSKNGNAAETSNKEATIDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLKAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(9509..9607)
                     /locus_tag="POB92_001159"
     CDS             complement(9509..9607)
                     /locus_tag="POB92_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578668.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
     gene            9692..11080
                     /locus_tag="POB92_001160"
     CDS             9692..11080
                     /locus_tag="POB92_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDI6578669.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(11161..11916)
                     /locus_tag="POB92_001161"
     CDS             complement(11161..11916)
                     /locus_tag="POB92_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDI6578670.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYNQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            12408..12977
                     /gene="ahpC"
                     /locus_tag="POB92_001162"
     CDS             12408..12977
                     /gene="ahpC"
                     /locus_tag="POB92_001162"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDI6578671.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            12993..14516
                     /gene="ahpF"
                     /locus_tag="POB92_001163"
     CDS             12993..14516
                     /gene="ahpF"
                     /locus_tag="POB92_001163"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI6578672.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDCNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            14631..16241
                     /locus_tag="POB92_001164"
     CDS             14631..16241
                     /locus_tag="POB92_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578673.1"
                     /translation="MLDVHFIYPRKLQNACEYFNLPTQKEGFEVTMHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQLNEDEFFVNNFFLRY
                     INSEVLDNAYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNATLFEFDILSSILESDDNYRL
                     NNELTKFEKLQLINFDNFLTIHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVNEQTIENSLAEFKNALELSFMEDEVDLDMLLEDEDFGTTDSLRSDAITQLIS
                     EIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLLQRFYDATFSKEELQYSLDTLA
                     GTELYYVEDDFVFHPVLFDVVESEASGIDISDDGYYVPASVEELIYYDRHHYLKSDQA
                     IKEITSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLIQPIMQLFIDNAILNNVQN
                     DALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            16314..16940
                     /locus_tag="POB92_001165"
     CDS             16314..16940
                     /locus_tag="POB92_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDI6578674.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILENKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            17195..17578
                     /locus_tag="POB92_001166"
     CDS             17195..17578
                     /locus_tag="POB92_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578675.1"
                     /translation="MKVRITFIILAILSTIVCLFLTAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFVSAIITLAIGGKSVDKNDFH"
     gene            complement(17644..18225)
                     /locus_tag="POB92_001167"
     CDS             complement(17644..18225)
                     /locus_tag="POB92_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6578676.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            18403..18612
                     /locus_tag="POB92_001168"
     CDS             18403..18612
                     /locus_tag="POB92_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578677.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(18651..18902)
                     /locus_tag="POB92_001169"
     CDS             complement(18651..18902)
                     /locus_tag="POB92_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6578678.1"
                     /translation="MFGFIGMLIIGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
BASE COUNT         6319 a         3531 c         2969 g         6302 t
ORIGIN      
        1 atctttacca aattcattat acataaaatg cttcaaatta taatagccaa cactatgtaa
       61 aagctccaca catttttctt ttgacttaat ttcaggaaaa tataaatttt tagtttctaa
      121 tttatataac atttgttcta ttgtaaaggg agtgggtatt tcacttttag gagcatcgtt
      181 tagtgtctca aaagttactt ttaaattatc atactcttta acgtcgatta ctttttcttt
      241 atacattatg ccttctaatt cttttaagtt aatttcaagt gatttatcca taactataaa
      301 ttctcctatt acaaaaaaaa ggccaactgt gtttcgcatt gctaagaggc gttagttgac
      361 ttctaataaa ttaatattaa tctaaggggt gtcaattgtc aattgtaatt caatcattct
      421 tgtcttctca aacctcacta aatcatcaat aaatataggt tctaacataa tcagatttac
      481 cttatcgcca aatattctat gataacgacc actttctttt atgtagtaca aaagatattt
      541 atttaaaact cttgtctctt agtaacaaat aaattttctc tcactcctct aacggatatc
      601 atctacaatc attgtatgac taggattgtc gttaaatact tcttctacat ttttgtctaa
      661 cttctcatca tctccgtctc aaaatataag aaagagatga ttcccattat ttcacgtcat
      721 gatgttgccc atgccaagca caccaaaaag gctggaaacc gcatagtaac ggtaactcca
      781 gcctatcatt tgctatatat aattattccc actcaattgt gcttggtggt tttgatgtaa
      841 tgtcatagac tacgcggttg acgtgatcta cttcgtttac gatacgacta gaaatctttt
      901 gtaagacttc ccaatcgatg cgtgcgaagt cacttgtcat accgtcgata gatgttactg
      961 cacgaatacc tactgtgtga tcatacgtac ggtagtctcc cataacacct actgattgaa
     1021 tgtttggtaa cactgtgaag tattgccaaa tttctctttc aagaccttct tctctaatca
     1081 cttggcgtaa aatcgcgtct gattctctaa cgatttctag tttatcttca gtaatttctc
     1141 caagtacacg aataccaaga ccaggtcctg ggaatggttg tctccacact aaatgttctg
     1201 gaatacctaa ttcaatacct aatttacgta cttcatcttt aaacaatgta ttgattggtt
     1261 cgattaattc gaattccatg tcttctggta atccaccaac attgtgatgt gatttgattg
     1321 tttgtgctgt cttggtacct gattcgataa cgtctgtata aagtgttcct tgcgcaagga
     1381 agtctacacc tttcagtttt gatgcttcat catcaaatac atatacaaat tcattaccaa
     1441 tgattttacg tttttgttca ggatctgaaa caccttttaa tttattcata aaacgatctt
     1501 tcgcattaac acgaataata ttcatgttga acccttcacc gaattgttcc ataaccatgt
     1561 cgccttcacc tttacgaagt aagccatggt ctacaaagat acatgttagt tgatccccaa
     1621 ttgctttatg caataataca gcaacaacag atgaatctac gccaccactc atcgcacata
     1681 atacacgacg gtcgcctacg cgttgacgaa tcttttcaat ttcaatttcg ataaagtttt
     1741 ccattgtcca ttgaccttta cactcacaaa cacgacgtac gaagttgttt aacaaatcat
     1801 taccatattc tgtatgacgt acttctggat ggaattgaac accataaatg cgacgtttct
     1861 tatcttcgat tgctgcatag tctgtacttg ggctatcagc gataacttca aagccttctg
     1921 gaatttcaat aactttatca gaatgactca tccaaacagt ttgttctgct ggtaagccag
     1981 cgaataactc atctgacttc gcattaatga ttgctttacc gtattcacgt tcattggcac
     2041 gctcaacttt accacctagt aatttagtag ttaattgcat gccgtaacaa ataccaagta
     2101 ctggaattcc taaattatat atttccggat caattgtaaa tgaaccttct tcataaactg
     2161 aatttggacc acctgataag ataatacctt ttggattcat tttcttaatt tcttcaattg
     2221 aaatttcatg atcgtgtaat tcactataaa cgcccatttc acgaattcgg cgtgtaatta
     2281 attggttgta ttggctacca aagtctaaga caaggattaa ctcttgttct tttgccattt
     2341 ccatatttgt cgttctcctt tatcttaatt agaatgagta gttcggtgat tctttcgtaa
     2401 tttgaatatt atgtgggtga ctttctgcta aaccagcagg gcccatacgt gtgaattgtg
     2461 cttcttcgcg taattctctt aaatcgtgtg aaccagtata acccatacca gcacgcacac
     2521 cgcccattaa ttggtaaatt gtatcttgta acgcaccttt atatgccgta cgtccttcga
     2581 taccttcagg aacaaatttc ttaggcgctt tgtcttcttg gaagtaacgg tcgtttgaac
     2641 ctttttccat cgcacctaaa gagcccatac cgcggtatac tttatattgt ctaccttgga
     2701 aaatttctgt tgcgcctggg ctttcttcag tacctgctaa taagctacct aacataaccg
     2761 catgtccacc agcagctaat gctttaatga tatctcctga gaatttaata ccaccatcag
     2821 caatgatagc tttaccatgt ttgcgtgctt cagttgcaca atcataaatt gctgtaattt
     2881 gtggtacacc aacacctgct acaacacgcg tcgtacaaat tgaacctggg ccaataccaa
     2941 ctttaacaat atctgcaccc gcttcaaata aatcttttgt tgcttctgca gttgctacgt
     3001 taccagctac taatgtgatt tctgggtaag tcttcttaat atgtttcact tgatcgataa
     3061 cgcctttaga gtgaccatgt gctgtatcga taactaagac atccacacct gcttcgacta
     3121 atttttgagc acgaatatca gtgtcttttg aaataccgat tgctgcggct acaagtagac
     3181 gaccatgttc atcttttgct gcattaggga attcgattac tttttcaata tctttaatag
     3241 taataagacc ttctagacgt ccgtctttaa ctaatggtaa cttttcaatc ttatgttttt
     3301 ggagaatttt ttctgcttct tcaagtgttg tattcactgg agctgtaatt aaattttctt
     3361 gcgtcattac atctacaatt ttaatcgaga agtcttcaat aaaacgtaag tcacggtttg
     3421 ttaaaatacc tactaagttg cgatcttctt tattatcaac aattggtaca cctgaaatac
     3481 ggtatttacc cattaatgct tctgcttcat aaacgctttc ttctggcgtt aagaaaaatg
     3541 ggtttgaaat gacaccattt tctgagcgtt ttactttttg aacttcgtcc gcttgttctt
     3601 caacgcccat atttttatga ataacaccta aaccaccttg acgagccata gcaatcgcca
     3661 ttttagattc agttacagta tccataccag cagaaataac tggaatattt aatttaactt
     3721 tgtctgataa ttgtacgctt aaatcaacgt ctttcggtaa aatatcagat tgtgctggaa
     3781 ttaataacac atcatcaaac gttaatgatt cttttgcaaa tttactttcc cacattaaaa
     3841 acagcctcca tttttcaaat taattagtta tattatttca cattttcttc cgtttgttta
     3901 tactttattc cattaaaaaa gaaattaaga ataattgcag agattgcgcc taaaacaatt
     3961 ccgttttgag tcaaccatgc aaattgttca cctaaacctt taaatgcttg tggtacagcg
     4021 cttataccag tacctaatcc tactgataca gcgataatta ataaattgtt ttgattttta
     4081 aaatcgatat gacctaatat actaacacca tatgccatta ccatgccaaa catagctatc
     4141 atcgcaccac ctaacacagg tagcggtatg atatttgcta atgcaccaag cttaggtata
     4201 caaccacata taagtaataa cacgaccatg ccgtatataa cattattttt cttagcgccg
     4261 gataaagaaa caagtcctac attttgcgaa taggctgtat acggaaatga attgaatata
     4321 gaacctaaca ctatcgctag accttccgca gtataacctt tacgaaaatc ttttctttct
     4381 aacttcttac cggtaatttc acttaacgca tgatagacac ctgtcgactc aattaaacta
     4441 acgatagcta caataaagaa cactaacgtc gatgtcacat caaagctaaa tccagagaat
     4501 ctaaacggca ctgggatgcc taaccaaccg gcatgattga cttgattaat atcgaccatc
     4561 ccaagtaagc cagcacctat cgttcctaaa acgagtccaa ttaatatggc aatactctta
     4621 ataaatccag ttgtgaatct ttgtaaaaca agaataatga ttaatgtcat taaacctaac
     4681 aaaatgttct taacatctcc atagtccttt gcaccttgac ctccagctaa gtaattcatt
     4741 gctactggca ttaaattgat accaatgata gtaacaacac tacccgttac tactggtggg
     4801 aagaatttta caagatgtga aaagaaaggc gcgatgataa taactaatat ccctgataaa
     4861 aatagcgaac cataaagtac atctattcct ttcgtttgac caattaaaat catgggcgca
     4921 acagccgtga atgtacatcc aagaacgatt ggtaatcctg ttcctgttac tttattggct
     4981 tgtaaaaatg tggcaacccc acacataaat atatctactg taactaagta agcgatttgt
     5041 tcaggtgtaa acttcaaact tgtaccaaca atgattggaa ctaagatggc acctgcgtac
     5101 atagctaaaa gatgttgaac acttaggatt aaatttttca ttattcttct cccaccaatg
     5161 tcactttgtt tccttctagt gaagcaacct tgcagagaga agaaactgtt aaacctgctt
     5221 cttctaaacg ttgatgccca ttttggaaac tcttttcaac aacaatacca ataccagctg
     5281 tcttagcatt cgcttgctgt gcgatatcgt ataatcctaa tgaagcatca ccatttgcta
     5341 aaaagtcatc gatgatgagt acagtatctt cttctgataa aaattctttt gaaacaatga
     5401 ccgtacttgt tttattttta gtaaatgaat gaatagatgt ttcataataa ccatccgtca
     5461 aagtgctagg ttttgctttt ttcgcaaata aacatggcac atcaaaatgc agtgcagcca
     5521 tgattgcagg tgcgataccg gaagcttcaa tggttaagat tttagtaatc cctttatctt
     5581 taaattgctc gtaaaaagtg cgaccaactt cattcattaa ctttgcatca atttgatgat
     5641 ttaaaaatcc atcgactttt aaaatcttct catcaatgac aacgccgtct tcctttactt
     5701 tttgtcctag taactccact cttaaaacct cctcagattt gtgtgaaaca ttttgctctt
     5761 aaattggtgc tagatacaaa aaaatcccca aactaaataa tagtttcagg gtttatgagt
     5821 gaacgaacat gcataacgaa tttgtcatgc aatcaatgta aaagaagttt catcaaataa
     5881 actgtgacca tcatataaaa tgatataaat cacccaccat ggttacaatt taatggctga
     5941 agctactcct agtattgtgt tgttactcat agtcatgtcg ttcaaggcaa catggtagaa
     6001 acttctaaag ccatattctt tagattatat gagtttatgt aaattattta acgataatag
     6061 caaattttcg gcattttttc aataactgct taggtaatct tttaatagtt ttataaatgt
     6121 acagagacgt ttaatgcgaa tgaagactta taacttaata atgtaagggt tttactttat
     6181 aataagaagg gtaaaagatt aaataaacaa atttttaaag gggcgacaac tatggcagat
     6241 attactgtag taaatgacac tggcgaatta tacaacgtaa tcaatcaaaa gaaatctgaa
     6301 ggttatttag aatcagagct aacaattatt agtaaaagta aattacacct aaatgactta
     6361 cacgactctg aaatctccct tatctcaaca agtggaacat ttagtgatag aatgactaaa
     6421 ctgttaacag gagaagatgg tgaacatgca gttttatcac gttacaactt agcgcctgat
     6481 gaattagaaa aatataaaca acttatttta gacgataaaa tgttagttgt agcggttcgt
     6541 gacaaatcat cacatcaaga agtgcatgaa aataattcag cttatgaaga gattgatatt
     6601 acacattttg ctgaagcatc taaaggtccc aaagcttagt tatcacactg aattcaaaat
     6661 gagattactg tactgtctaa tttaagatag tacagttttt tataccattt aaaagtttat
     6721 gaacagtctt ttacagcagg cgattatgct ataatattat ttaaattggg gaggtgttct
     6781 tatggaattt aaaaccattc aatctgtcaa agatccacat tacaacgaag cacttaaact
     6841 ttacgatact aagttaaatt tatctttaac tgaagataaa cgtatcatca aacgctcatt
     6901 agaaaacaac aaaaccgaaa acgattatgt ctttatcgta ggcttagaaa acgatgttgt
     6961 tgtcagtcta gcaactgcac attatgaagc cacaaccaat tcagcatttt taatttattt
     7021 attcgcaaaa gacctaccca accatgatga gttaatgacg caaacattac atcaaattga
     7081 acatgaacta aacttattat caaaccttgt tcattctaga agtattaact ttttaatgat
     7141 ggaagtccct aaagaacctg cagatttaac acaagataat ggactcatta ttgaacaccg
     7201 tagacagttt ttatacgaac atcaatttga aagacaaact gaaattgatt acattcatcc
     7261 aactaatgat ggtcacacta aaaaagtgga cttattttta aagaaaaata ttaaactgac
     7321 aaaggatatc tacggtacaa gtgttaaatc aaattatatt ttgaagtatg tatttgcaaa
     7381 tggtatcgca cgtgaaatta tttatgcttt attagaagaa atggatttac gtaaaccatt
     7441 ttaaacaaac ttgaaatcac taacaataac gtttaaacta tgtatgaagc aagactgacg
     7501 tttcactgcc cgtagacaaa cgtcagtata cataaagcat atgcagaaag gactttaaag
     7561 tatgttaact aaagaatttg cacaacgcgt agaactaagt gagaagcaag tccgtaaaat
     7621 tgttcaacat ttagaagaac gtggttacca actaagcaag actgaatatc gtggtcgtga
     7681 agcaactgat ttcaaagaag aagatatcga gcttttcaaa gacattgccg acaaagtaaa
     7741 acaaacaaat agttatgatc tagcgtttga tgaattagaa aaagaaaaag acttcctgca
     7801 agtcattgtc aaaaacgatg acaaaaactt acctactaat caaaatgtcg ctcaattagt
     7861 agaagattta cgcctagaaa tccagaaaat gcgcgaagaa cgtcacctac ttggtcaaat
     7921 gatgaatcaa gtacatcagc aacaacaaga attaaaagaa cttcaaaatc aacttacatc
     7981 taaaatcgat tcaaatagcg aatccttaaa agctatccaa acatcacaag aggctatcca
     8041 agaagcacaa gcatctcaag ctaaagtatt ggctgaatca acaaataaag ttgaaaagaa
     8101 tgctgttact gaagataaag ctgacagtaa agattcgaag gttgctggag tgaatacgtc
     8161 aacagacgct aagacggata ctaaagcaga caatgcaggc gatggcacta ctactaaagt
     8221 agataaagag gatcaaattt ctgcaactga agcaattgaa aaagccagcg ttgagcagtc
     8281 taaaaatggc aatgcagctg agacttcaaa taaagaagct acaatagatg cagaagcgca
     8341 acatgatgct gaacaacaag tagcagaagc ccatgcagaa gcttctaaac aagctacttc
     8401 gaacgattca ttgaaagcga aagcagaaaa tgacagcaca gcttcacagt ctgaaatgtc
     8461 agaaccaaaa cctcaagaag agaaaaaagg tttcttcgca cgtttattca acttgtaggg
     8521 agtgggacag aaatgatatt ttcgcaaaat ttatttcgtc gtcccacccc aacttgcatt
     8581 gtctgtagaa atcgggaatc caatttctct ttgttggggc cccgccggca aggttgacta
     8641 gaattgaaaa aagcttgtta caagcgcatt ttcgttcagt caactactgc caatataact
     8701 tcgtagagca tagaacattg atttatgtcc cagcctgtaa tactcaataa tatatagctt
     8761 tgatttaaaa actgatcttt tataaaacta tagaactgta atttactttc aaatttaata
     8821 ctcaattcat aaaacctgaa acaagctgat atactccttt caatatctac tttgaagaga
     8881 gcatatcaaa ctaatttgtt tcaggttttt taattattca ttatgattaa tattgaattt
     8941 tgaataaatt tttgtagtat gttactttta aatgggatgg cttctgtgta ttcctaaatt
     9001 aattcatctt ataagcccat tcaatttaaa tgtttggctg tgttagtaac tgacgaatcg
     9061 cacaagggca cactcatatt cacattttct acaaacgcct ttttgctcaa acaaaaatat
     9121 ttgttttacg gtagcttttg aactaccacc tgcacacacg aaccctcaaa agatgctaat
     9181 tttgattcga cagaattttt tacaacgtgt ttgttcgaag tgtcttttca aggttgatca
     9241 tttattatct ttttattcaa taaatatgag gttagcaatt tttattaaac cgccatagcc
     9301 acatctcgat tcaaggatac atttgttttc tcatatctta tctctcatga cagtacacga
     9361 tatatatctt ttccaaatat tcatcacttt tttcccgtta aaatcatttc tattattaac
     9421 gctgtaatga caacttattt aatttattgc gtttaattcc ctgcttcatt ttcaaatatc
     9481 aactttaatc gactaggtca taacactatt aacccttttc acctttctct actaaattca
     9541 aaacgatttg tttacaaatt cccttatgcc aacggtcgaa tttcatcatg tttacattta
     9601 attgcaaatg ccctcttgaa atattttaaa attaatatta ttcttagcat actagtcgga
     9661 attatattca aaaatttgag gagtgacgtt catgaatgct tttcttacac tgattaatat
     9721 tattgtattg gtgattttca tcgtaatatt gcacatgatg gctcgcaaac atatatcttt
     9781 tgcgaaacgt gtgtttacag ctttaggtat cggcattgta tttggcgtgt tattacatct
     9841 tgtctatgga acacattcga atgttattac atcaacaagt gactggttca acattgtcgg
     9901 acaaggttac gttgcattgt tgcaaatgat tgtgatgcct ttaattttca tttctattgt
     9961 cgctgcattc actaaaattc aaatcggaga aaagtttgct aaaattggta gtctcatttt
    10021 tatcttttta attggcactg tcaccattgc tgcaattgtt ggtgttgttt atgccttggt
    10081 gtttggttta gatgcatcaa caattaacct tggtaacgct gaacaagcgc gtggaagcga
    10141 aattgctaaa caagcgaaag atttaacagc acatacgtta ccacaacaaa ttttggaatt
    10201 attgcctaaa aatccattct tagatttcac tggacaacgt gcaacttcca cgattgctgt
    10261 cgtgatattt gcttcgttta taggatttgc ttatttacgc gtagcacgca aacaacctga
    10321 tcatggtgaa ttgcttaagc gtgcgattga cgctatctat tcactagtaa tggcgattgt
    10381 gacgttcgtc ttacgtttaa cgccatacgg tgtattagca attatggcca acacattatc
    10441 gacaagtgac tttggcgcca tttggacttt aggaaaattc ttaatcgcat cgtacgctgc
    10501 attaattacg atgtatatca ttcacttaat tattttaagc ctgttaggta ttagcccaat
    10561 tcgctatgtt aaaaagactt tggaagtatt aatctttgca ttcacatcac gctcaagcgc
    10621 gggtgcctta ccattgaatg tacaaacaca aacaagacgt ctaggcgtgc cagaaggtat
    10681 tgccaatttc gctgcgacat ttggattgtc gattggacaa aatggttgtg caggcattta
    10741 ccctgccatg cttgcaatta tggtcgcacc cgtcgctaat gtagaaattg atttacaatt
    10801 tattgtaacg ttaatcgctg tcgtgattat tagttcattc ggcgttgctg gcgtcggtgg
    10861 cggggctaca tttgcatcca tattggtgtt gtcaacgtta aacttgccag tcgctttagc
    10921 cggcgtgctc atctccgttg aaccattgat tgacatgggt agaacagcat taaacgtgaa
    10981 cgattcaatg ctagctggta caggtaccgc caagttaact aaacattggg ataaagatac
    11041 tttcgattca aatgataatg ctgcattaac ttcacactaa ttcattgcaa gaaatacccg
    11101 ccccctctag ctatacttat cattttaagc tagagggggg cgggttattt tggtatcttg
    11161 ctatcgctgt attaagcctg atttttgcaa ttgttctaac atatctaatc ttgctttgtt
    11221 tcctaggaac atctcaattt gctgcgacca tgtttctttg cgattcccgt ttgtacgttg
    11281 attatagtac tcgctgattg tctggtcgta atctgccatt tgtgcatact gtgtttcctt
    11341 atcagcatga tacttattat gatggaagac atggtcaaat ggtaagcgtg gcttggctgc
    11401 accattttcg tcatctgcgg gttcccctac tgccatacca aataccggga agacatagtc
    11461 aggtaagtct aaaatttctc gaactcgttc aacatcattt cttaatgatc ctaaaaagac
    11521 aatgccatac cccatatctt cagcagttac cgcaatattt tcggcaacta atgctgcatc
    11581 gattgcacct actagcaaac cttccgttga accatatgca ttttccatat cagtttcagc
    11641 atgttgatca actaaatgat gacgataata atcaataaca aagacgaata aatagccatt
    11701 ttctacaaca taaggttgtc cagaaacttc tcgtaaattt tctttaatct tctcatcgtc
    11761 gataccaata attgagtatg cttgcaggaa acttgacgtc gaagcgcttt gtccagcttc
    11821 taccaatttc ttaacaacgt cttcacttaa aggtttattc ttaaattttc taacagaatg
    11881 atgctttttc acaagattat atacatgttc tgacacgtta cccactcctc gatactttac
    11941 aattaattta aaatcttcat attgcaattg tacacgtcca aactttttta tagcaattat
    12001 ttgcttataa gttaataatt gaacttttaa aaatattgtc tcatacttta aaaatccagt
    12061 ttctcactca ttaccttcat ccatctcgaa caaatattct cacaccatta tcaataaggt
    12121 tgacttagtt gagaatacaa atcttctttt tttacatttt ttacattaaa atcaagatat
    12181 taaatataga cacaaccttt taaatcatca actacaaaat ccattatcaa agcactttat
    12241 attatctttc tttattttaa aagtaatcat tcactttaca aagttatcca gttgactatt
    12301 ctggatcaaa atgtaataat gctaactaag agattagaat tattataatt aagcacaatc
    12361 aattgatagt tctaattcat acttaaattc ttaggaggaa gatatttatg tcattaatta
    12421 acaaagaaat cttaccattt acagcgcaag ctttcgatcc aaaaaaagat caatttaaag
    12481 aagttacaca agaagattta aaaggttctt ggagcgtagt atgcttctat cctgctgact
    12541 tctcattcgt ttgtccaact gaattagaag acttacaaaa ccaatatgaa gaattacaaa
    12601 aattaggcgt aaatgtattc tcagtatcaa ctgatactca cttcgtacac aaagcatggc
    12661 atgaccattc agatgcaatt agcaaaatca cttacactat gattggtgac ccatcacaaa
    12721 caatcactcg taattttgat gtattagatg aagctactgg tttagctcaa cgtggtacat
    12781 tcattatcga cccagacggt gttgtacaag catctgaaat taacgctgac ggaattggcc
    12841 gtgacgctag tacattagct cacaaaatca aagcagctca atatgttcgt aaaaaccctg
    12901 gcgaagtatg cccagctaaa tgggaagaag gcgctaaaac attgcaacct ggtttagatt
    12961 tagtaggtaa aatctaagga ggcattacaa ccatgcttaa tgctgattta aaacaacaac
    13021 ttaaacaatt attagaacta atggagggca acgttgaatt cgttgccagc cttggttcag
    13081 atgaaaaatc taaggaactc aaagaattat taacagaaat ttctgatatg tcacctagac
    13141 tttcactttc tgaaaagtct ttaaagcgta caccaagttt ctcagtcaat cgtcctggcg
    13201 aagaaacagg tgtaacattt gcaggtattc cattaggtca cgagtttaac tcacttgttt
    13261 tagcaatttt acaggttagt ggtcgtgcac ctaaagaaaa acagtcaatc attgaccaaa
    13321 ttaaaaactt agaaggttca ttccattttg aaacattcat tagtttaacg tgtcaaaaat
    13381 gtcctgatgt cgttcaagca cttaacttaa tgagtgtgat caaccctaac atcacgcatt
    13441 ctatgattga tggtgcagtg ttccgtgaag aatctgaaaa catcatggca gtccctgctg
    13501 tctttttaaa tggcgaagaa tttggcaatg gtcgtatgac aatccaagat attctttcga
    13561 aactaggcag tacggcagat gcatctgagt ttgaaaataa agaaccttat gatgtcttaa
    13621 tcgttggtgg tggtcctgct agtggtagtg cagcgattta cacagcacgt aaaggtttac
    13681 gtactggtat agttgctgat cgtatcggtg gccaagttaa tgatactgct ggtattgaga
    13741 acttcattac tgttaaagaa acaactggtt ctgaattttc ttctaactta gcagcgcaca
    13801 ttgatcaata tgacattgat gcaatgacag gtatacgtgc tacagatatc gaaaagactg
    13861 acgaagcaat taaagttacg ttagaaaacg gtgctgtctt agaaagtaaa acagtcatta
    13921 ttgctactgg tgcaggttgg cgtaagctaa acattccagg tgaagagcaa ttgattaata
    13981 aaggtgttgc attctgccct cactgtgacg gacctctatt tgaaaataaa gacgtagcag
    14041 ttatcggtgg cggtaactct ggggttgaag cagcaattga ccttgctggt atcgttaatc
    14101 atgttacatt attcgaattc gctagcgaat taaaagcaga caacgtgtta caagatcgtt
    14161 tacgttcttt atcaaatgtt gatatcaaaa caaatgccaa aactactgaa gttgtcggag
    14221 aagaccatgt tacaggtata cgttacgaag acatgagcac cggcgaagaa catctactta
    14281 acttagatgg tatctttgtt caaattggtt tacttccaaa cacatcatgg ttaaaagatg
    14341 ctgttgaatt aaacgaacgt ggtgaaattg ttattgattg taacaataat actaacgtac
    14401 ctggaatatt tgctgctggc gatgtcacag atcagaagaa caaacaaatt attatttcaa
    14461 tgggcgctgg tgcaaatgca gcattaaatg cctttgacta tattatcaga aactaaagtt
    14521 tcattcattt cactgacagt atcttacatt tgttaagatg ctgttatttt tttatcccga
    14581 cataaaatca tgttatgata aacttaatat atacaacgag gtgtgcaaaa atgcttgatg
    14641 ttcattttat atacccaaga aaattacaaa acgcatgcga atattttaat ttaccgaccc
    14701 aaaaagaagg atttgaagtc acgatgcatg ctttacgccc tgaaaatata cataaaattt
    14761 atcatgatat ggataagaca gaacaaaaat ttatagaaac acttatatct aatggtaatc
    14821 ctgtcacaat tataaataaa cgcgatgtta atattttaaa gctaacaaat catctaggtt
    14881 ttgtttatca acttaacgaa gatgagttct ttgtaaataa tttcttttta agatatatca
    14941 attcagaggt actagataat gcatatggtg aattcgaatc gttagataca ataaaccata
    15001 tgaatttaaa tgaagaagtg ttacgtgtaa gacaacatac gcgcttaaac gatgtcacat
    15061 tagaacatgc tttactgtta aaaactaatg acgagctaaa aacaatatgc aggcagtatg
    15121 gtatcaaagg attctcaaat aaaaacaaac aaaatcttgt tgaattaatt atgacagcat
    15181 ttttcaataa taatcacatc attgaacaag tgttttctaa tgctactcta tttgagtttg
    15241 atattctttc tagcatactt gaaagtgatg ataactaccg attaaacaat gaactaacta
    15301 aatttgaaaa gctgcaatta ataaattttg ataacttttt aactattcat ttttattatt
    15361 attacgactt tgaatatgat tgtcttattt tgccaaatga tgtgcgtaaa catattgaaa
    15421 atttcgtgaa tcaagtcggc aatggtgatt ttgagaaagc tgtaaatcaa tttgttaatg
    15481 agcaaacaat tgagaatagt ttagcagagt ttaaaaacgc gttagaatta tcatttatgg
    15541 aagacgaagt tgacttagat atgttacttg aagatgaaga ttttggtaca accgatagct
    15601 taagaagtga tgcaatcact caattgattt ctgagattaa gaaaggatta atagataaaa
    15661 aagatatttt acctcttcgt attatacttg gttctgtaaa cttgtatggt attttatcaa
    15721 ctaatcatct actatatctt ttacaaagat tttatgacgc tacttttagt aaagaagagt
    15781 tacaatatag cttagatact ttagctggta ctgagctata ttatgttgaa gatgacttcg
    15841 tgtttcatcc tgtgttattt gatgttgtcg agtctgaggc gtctggcatt gatatttctg
    15901 atgatggata ttatgtacca gcttcggttg aagaattaat ctattatgat agacatcact
    15961 atttgaaatc tgatcaagca attaaagaga tcaccagtta tctacgcaaa caaatcaata
    16021 tgaaaaatga tattgagaag gaaaagtatg tgaacgaaat cattatgctt ttacgtacga
    16081 tacctacgtc taaattgata caaccaatta tgcagttatt tatagataat gctatcctta
    16141 acaacgttca aaacgacgca ttattgattg aaaaagcaac tcatgcacgt aatcatttac
    16201 gtctatggtc attgcaaggt catcgtgaca ttccaaacta attcaatgtt actctattaa
    16261 acatgaatac acattttttc aattaaagat aaactaagga gttatattac attatgaaaa
    16321 agagattact actaagtaca tttttagcat ctgcattaat tcttacaggt tgcgcgtccg
    16381 atcaatctga taacgaagat catcacacaa gtactggcat ccatgcacct aaaagcgcta
    16441 aaaaattaga aacaaaagat atctttaact cagataagaa aaatagtgat attagtgatg
    16501 cagagatgaa gcaagctata gaaaagtatt tgtctgtaaa tagcgatata cttgagaaca
    16561 aatatattat gcaacataaa ctagataaac aaattgacag tcaaacaaaa gtgactgaaa
    16621 aacaagcaga aacacttagc cacttatcta acttagctgt taaaaacgat ttgcatttca
    16681 agaaatttgt cactgaaaac aatattccta aagaatataa gaaacccgtt gaattaatga
    16741 tgaattactt caaagcttta aatagtacaa tcgccaatgt tgatgaagat attgaaaagc
    16801 ttagctatca accgcaaaat aaaatcaatg tagtcgacgt gccaacaaaa tatgccggtg
    16861 atgttaataa aaagcaacaa gataaaatta aagatttctt aaaatctaaa ggtatcaaga
    16921 gtgatgttat cgataaataa ctgaaagcca tcattgcgac atgtaacaca tcattacatg
    16981 agctttaatt catgacaatt ctttatcttt aaaagtgact aatcaattta gctaaagtca
    17041 tcatttgggc atcaagcagc atattactgt ttgatgcttt tttaattgcc ttaaattcac
    17101 tatccatcct aaaattccca ttagttgacg catttgacta tatacctaac atattccata
    17161 cttatattga ctctataaac aaaggatgat catgatgaaa gttcgaatta cttttattat
    17221 tttagcaatt ttatcgacga ttgtttgctt gttcttaaca gcaatgcatc caacaggacc
    17281 aaacacagta acttttgaac agccatattt attcaccctc aacatcatta taatggtatt
    17341 agtcgcatta ccttcacttg ttttagctat atatgattat atgagtttta gaatttcttc
    17401 tgctatttta caatttctag gggctatctc ttggttcttt ttatcattga tattatcgct
    17461 cacacaattt acacctttta cattagcgtc atttataact tcaattattt tgttcgtaag
    17521 cgcaattatc acattagcta ttggtggtaa gtctgttgat aagaatgact tccattaaat
    17581 tccaaataca aaaaaggttc tgaaggcagc tataaaacac agcttttcag aacctctata
    17641 cttctattca atgatatatg gtttgcaatt ttcaactttt aaatccacag cttcttccct
    17701 tgaaactttg ttaaaataaa ccatcaaaca acgaatgaca acttgatgtg caacaatgac
    17761 gatatcatct ttttgtgtat cttcattgac aacatgattc ataaaatgtt cgacgcgttg
    17821 atatacatct tcataacttt ctccttcagg cgctttttgt gaaaaactat gacgaaagtc
    17881 tttaaagttt ggatcattga aatatttttc atatttcgga ttcgcactga tttcatcttt
    17941 atattcaccc tcaaatacgc caagtgaacg ttctcttaat agaggcgtag tcgttgatgc
    18001 aatgtcatat ggaaaaatat gttcaaacgt ttgctgtgtt cttaataagt ctgaaacata
    18061 tacatgttta atctgtttct ctttaaaata atcacataaa tcgtctgcac tttttgtgcc
    18121 agtatccgtt aacggcacat ctaattgtcc acaaaaataa gatcgaaaat gtttattatc
    18181 ataattcgat tttgattcgc catgtctaac taaataaatc gtcataatat tactccttac
    18241 cttatgtatt tcatatctac cataacactt tgactactaa ttcgatatca atcttaatat
    18301 tctattctaa aaaaagaatt aattcatatt ttaaattaat gactctaaaa atagtattgt
    18361 ctttaatcaa gtgatgtgtt actatatggg tgtagttctt aaatgactac acagtctttt
    18421 atattcatga acataatact cctggatggc gcaacccaac gtcatccttt ttttatttgt
    18481 caaatactcg cttttttatt tccaaaattt cctcatatta atttattact ttttaatcct
    18541 atcttatcta cattttcaaa tattataata catattgacc attttcacgc aaataaaaaa
    18601 gatgacaact gaatgccatc tttatttcaa tttccaattg aattttttta ttatttacgc
    18661 atagctctta aaattaacgt tacgattgca attaagataa ttgaaccaat taatgctggc
    18721 aagatgtaaa tacttcctaa ttcaggaccc cattgtccga atagtttgcc acctacccat
    18781 gatccaataa tacctgcgat aatattgcct aaaataccac ctgggatatc tttacccata
    18841 atagcaccag cagcccatcc aattaagcca ccgataatta acattccaat aaatccaaac
    18901 ataattttca gtctcctttt tctatttatt ttgcgttatt ctaagtagta ccccttattt
    18961 acaattctaa aacaaattca aattattttt atccaaatat ttttaaaagt agtaattgaa
    19021 tatcaatttt attcaatgta gctatcgtta tttaaagtct ctgtaccggc aatatcatat
    19081 acattatttt ttctgccgaa ttcatagctt gattatttta t
//
LOCUS       DAMZLE010000037        18869 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMZLE010000037 DAMZLE010000000
VERSION     DAMZLE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.037"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            102..1763
                     /locus_tag="POB92_001170"
     CDS             102..1763
                     /locus_tag="POB92_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI6578679.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPFITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            1760..2455
                     /locus_tag="POB92_001171"
     CDS             1760..2455
                     /locus_tag="POB92_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI6578680.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKDYISATEDHHSKTVIDLIPEYQHLNIFTVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            2472..2894
                     /locus_tag="POB92_001172"
     CDS             2472..2894
                     /locus_tag="POB92_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6578681.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            3587..4996
                     /gene="sapep"
                     /locus_tag="POB92_001173"
     CDS             3587..4996
                     /gene="sapep"
                     /locus_tag="POB92_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDI6578682.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFDSDFGEKMGMKFHTDVMGDVTNNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            5000..5848
                     /gene="dat"
                     /locus_tag="POB92_001174"
     CDS             5000..5848
                     /gene="dat"
                     /locus_tag="POB92_001174"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDI6578683.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGVIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            6399..7190
                     /locus_tag="POB92_001175"
     CDS             6399..7190
                     /locus_tag="POB92_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDI6578684.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNQVMNSNMFI"
     gene            7205..7849
                     /gene="trmB"
                     /locus_tag="POB92_001176"
     CDS             7205..7849
                     /gene="trmB"
                     /locus_tag="POB92_001176"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI6578685.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMSLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSNKGSRIYRMEAKFHSQK"
     gene            8319..9161
                     /locus_tag="POB92_001177"
     CDS             8319..9161
                     /locus_tag="POB92_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6578686.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIITDLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIATNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(9286..9597)
                     /locus_tag="POB92_001178"
     CDS             complement(9286..9597)
                     /locus_tag="POB92_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6578687.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQYINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            9684..10760
                     /locus_tag="POB92_001179"
     CDS             9684..10760
                     /locus_tag="POB92_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6578688.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGSQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            10825..11136
                     /locus_tag="POB92_001180"
     CDS             10825..11136
                     /locus_tag="POB92_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6578689.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            11236..12093
                     /locus_tag="POB92_001181"
     CDS             11236..12093
                     /locus_tag="POB92_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDI6578690.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            12122..12718
                     /locus_tag="POB92_001182"
     CDS             12122..12718
                     /locus_tag="POB92_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDI6578691.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            12739..16563
                     /locus_tag="POB92_001183"
     CDS             12739..16563
                     /locus_tag="POB92_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6578692.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEADEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLNVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKPASQAAPSSRFDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLEDEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            16587..17900
                     /locus_tag="POB92_001184"
     CDS             16587..17900
                     /locus_tag="POB92_001184"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI6578693.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            17974..18465
                     /locus_tag="POB92_001185"
     CDS             17974..18465
                     /locus_tag="POB92_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDI6578694.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            18539..>18869
                     /locus_tag="POB92_001186"
     CDS             18539..>18869
                     /locus_tag="POB92_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6578695.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEE"
BASE COUNT         6970 a         2578 c         3528 g         5793 t
ORIGIN      
        1 ataaagtttt gtgatatatt cataataatg aaaaatgatt ttacagagaa atagctttta
       61 gtttacaaaa ctttaataaa catgagatag ggagattcgt aatgagtgaa agtaaagaaa
      121 tggtgcgtgg aaccttttta attaccatta gtatattaat taccaaggta ttgggcgtac
      181 tttttatcat tccattcaac tatttaattg gtggacaaga aaatatggcg ccgttcacat
      241 acgcttatgc accatataat attgcaattg cagttgcgac agcaggtgta ccattagcag
      301 cttcgaaata tgttgcgaaa tataatgcta ttggggcata taaagtcagt cagaaatttt
      361 ataaatcgag ttttattgta atgagtatta ccggtgtttt aggtttcttg gtactgtatt
      421 tcctagcacc ttatatttct gaattaacat tagctagaaa cgttcatgat aaaaacggtt
      481 ggtcagtcga tgatattact tggattataa gaatcattag tatggttgtt atctttattc
      541 ctgtattagc gacatggaga ggtatattcc aaggttataa atctatgggg ccaacagcag
      601 tgtcagaagt aactgagcaa attgcacgcg tgattttcat attaatcgga agttatttag
      661 tactaaatgt ctttgatggc tctattttat tagccaatgg tattgctact tttgcagcag
      721 cagttggtgc gattattggt atattcacac tctggtatta ctggagaaag cgtaagcata
      781 atattgatcg tatggtcgaa tctgattaca cagatataga tgtgtcatat ggcaagatgt
      841 ataaagaaat tattgcttat agtattccgt tcgttattgt aagtttgaat tatccattat
      901 ttaacttagt agatcaattt acacataacg gtgctttatc tttagttggt ataccttcac
      961 agttacaaga tatattcttt aatatgctaa atatgtcaac taataaaatt gtaatgattc
     1021 cgacatcatt aagtgctggt tttgcagtga gtttaattcc ttttattaca aaaacatttg
     1081 cagaaggtcg cttacatgaa atgcatcatc aaattagaac atctattggt gtattaatgt
     1141 ttataactgt tccagctagt attggaatta tggccttggc acaaccatta ttcacagttt
     1201 tctatggtta cgatccaatt gttttaggtc atgaccctaa ccatgatggt agtcgcttat
     1261 tattctatta tgcgcctgta gcaatactaa tttcactatt aagtgtaacg gcatcaatgt
     1321 tacaaggtat agataaacag aaattaacag tttatgttat tttagcttct gtagttatca
     1381 agttagcgtt aaactatcca ttaattatgt tattccatac acctggtgca attttaagta
     1441 ctagtatcgc attattattt gcaattggat gtaatttcta tattcttaaa aaatatgcga
     1501 agtttaaatt cagctatagt tggattcatt ttgcaaaaat attcttgtat tcatttatta
     1561 tgatgctagg tgtagaatta gtattcttcc ttgcaaatct attcttagaa cctactaaac
     1621 ttggttactt aatcattatt attttaggtg ttacagtagg tattctaatt tacggtacaa
     1681 tcacaattaa aacgcgtctt gcggacgaat tcttaggtga aattcctgaa aaattaagac
     1741 gtagagttag gtttttacga tgagaataga taaattttta gcaaatatgg gcgtcggtac
     1801 acgtaatgag gtcaaacagt tacttaaaaa aggccttgta aatgtcaacg aacaagtaat
     1861 taaatcacca aaaacacata tagaaccaga aaacgataag ataacagttc gtggagaatt
     1921 aatagaatac attgaaaatg tctatattat gttaaataag ccaaaagatt atatatcagc
     1981 aactgaagat catcattcaa aaaccgttat tgatttaata cctgaatacc aacatctaaa
     2041 tatttttacg gttggtcgtc ttgataaaga tacagaaggg cttttattga taacgaatga
     2101 tggtgatttt aatcatgaat taatgagtcc aaacaaacat gtttctaaaa aatatgaagt
     2161 tatttcagca aatcctatca ctgaagacga cattcaagct tttaaagagg gtgtaacact
     2221 gtctgatggc aaggttaagc ctgcaatttt aacttatatt gataatcaaa cgtcacacgt
     2281 aacaatttat gaaggaaaat atcatcaagt taaacgaatg tttcatagca ttcaaaatga
     2341 ggtattacat ttaagacgta tcaaaattgc tgatttagaa ttggacagca atctagattc
     2401 aggagaatat cgtttattaa ctgaaaatga ttttgacaaa ttaaattata aataaaaggg
     2461 agacgattga tatggcaaaa ggtaatttat ttaaagcgat tttgggtata ggtggcgctg
     2521 tagcagctgt acttgttaca cgtaaagata gtcgtgacaa gctgaaagca gaatataata
     2581 aatataaaca agatcctcaa agctataaag ataatgctaa ggataaagcg acgcaattag
     2641 gaacaattgc taatgaaaca attaaagaag taaaaacaaa tccgaaagaa tatgctaata
     2701 gattaaaaaa taatccaaaa gcatttttcg aagaagaaaa atcaaaattt accgaatatg
     2761 acaataagac tgacgaaagt attgaaaaag gtaaatttga tgatgaaggt ggcgccgcac
     2821 caaataataa tttacgtatc gtcactgaag aagacttaaa aaagaataaa aatgcactat
     2881 ctgataaaga ataagatgtg agtaggacag aaatgataag ggagtgggac agaaatcata
     2941 ttttagcaaa atttatttcg tcgtcccacc ccggcaaggt tgactagaat tgaaaaaaag
     3001 cttgttacaa gcgtattttc attcagtcaa ctactgccaa tataatattg taaactatag
     3061 gacatttatt agtgtttcag gttctttatc gttagtcata gctaatatgc atatagaaag
     3121 aagaatacat gcgtgaaact cattcataaa aaatactaat atctaaagaa aacgtatttc
     3181 tttatctttt cgaaattctc tatgttgggg cccttacccc aacttgcaca ttattgaaag
     3241 ctgactgttg gccagtttct gtgttggggc ctcgccaact tgcattgtct gtagaatttc
     3301 ttttcgaaat tctctatgtt gggggccccg gggcgcattt tcgttcggtt aattactgtc
     3361 aatattaaat tgtgtgccta gtacattgac ttatatctcg agttcttgaa tatgtgtatt
     3421 ttaaatatat aaattaaaaa aggaacaaag cagctattta tgatttgtgt catatcgata
     3481 gcatcgtttt gttccttttt attattttct gacatcttat tgtagttttt atatcatttc
     3541 atgtaaaata gaatttaact tatagaaaaa aggaagtcga tttcgaatgt ggaaagaaaa
     3601 agttcaacaa tacgaagatc aaatcattaa tgacttaaaa ggattattag caattgaaag
     3661 tgtgagagat gatgcaaaag catcagaaga cgcaccagtt ggtccaggtc ctcgtaaggc
     3721 attagactac atgtatgaaa ttgcacatag agatggattt acaacacatg atgtggatca
     3781 tattgcagga agaattgagg caggtaaagg aaatgacgta ttaggtatct tatgtcatgt
     3841 tgacgttgtt cctgctggtg atggatggga tagtaatccg ttcgagccgg ttgtaacaga
     3901 agatgctatc atagctagag gtacacttga tgacaaaggt ccaacaattg ctgcttatta
     3961 tgcaattaag atattagaag atatgaatgt ggattggaag aaacgtattc atatgattat
     4021 tggtacggat gaagaatctg attggaaatg tacggatcgc tattttaaaa cagaagaaat
     4081 gccaacatta ggttttgcac cagatgcaga atttccgtgt attcatggtg aaaaaggcat
     4141 tacaacattt gacttagttc aaaataagct tgctgaagat caagatgaac ctgattatga
     4201 attaataact tttaaatctg gtgaacgtta caacatggtg cctgatcatg cagaagcaag
     4261 agtgcttgtt aaagaaaata tgacagatgt tattcaagac tttgagtact ttttagaaca
     4321 aaatcattta caaggtgata gtactgttga tagtggcatt ctagttttaa cagttgaagg
     4381 taaagcggtt catggtatgg atccatctat cggtgtgaat gcgggtcttt acttactaaa
     4441 attcttggca tcattaaatc ttgataataa tgcacaagcg tttgtagcat ttagtaatcg
     4501 ctacttattt gattcagatt ttggtgaaaa gatgggaatg aaattccata cagatgtcat
     4561 gggtgacgtg acaaataaca ttggtgttat tacatatgat aatgaaaatg cgggtctttt
     4621 cggtatcaac ttgcgctatc cagaaggatt tgaatttgaa aaagctatgg atcgttttgc
     4681 aaatgagatt caacaatatg gctttgaagt gaaattaggt aaagtccaac caccacatta
     4741 tgttgataaa aatgatcctt ttgtacaaaa gttagttact gcatatagaa atcaaacaaa
     4801 tgatatgact gaaccttata ctataggtgg cggtacttat gcgagaaact tagacaaggg
     4861 tgtagcattt ggcgcaatgt ttagtgattc tgaagattta atgcatcaga aaaatgaata
     4921 tatcactaaa aaacagttat ttaacgcaac tagtatttac ttagaagcaa tttattcatt
     4981 atgcgtggag gaataatata tggaaaaaat ttttttaaat ggtgagtttg taagtccaag
     5041 tgaagcaaag gtttcataca acgacagagg atacgtattt ggcgatggta tttatgaata
     5101 cattcgagta tataatggta agttatttac agtaacagaa cattatgaaa gatttttacg
     5161 tagtgccaat gagattggtt tagatttaaa ttattctgta gaagaattaa ttgaactatc
     5221 tcgtaaatta gttgatatga atcaaattga aactggggta atttatattc aagcaacgcg
     5281 aggtgtagct gaaagaaatc atagcttccc aacacctgaa gtagaaccag caattgttgc
     5341 ttatacaaag agttatgatc gtccttatga tcatttagaa aatggtgtga atggtgttac
     5401 cgttgaagat atccgatggt tacgttgcga cattaaaagc ttgaatttat taggaaatgt
     5461 attagcaaaa gaatatgctg taaaatacaa tgccgttgaa gcaatccaac atcgaggcga
     5521 aactgtcact gaaggatctt caagtaatgc ttatgcaatt aaagacggtg tgatttatac
     5581 acatccgatt aacaactata ttcttaatgg tattacacga attgtaatta aaaaaattgc
     5641 cgaagactat aatatcccat ttaaagaaga aacgtttact gtagatttct tgagaaacgc
     5701 agatgaagtt attgtttcaa gtacttcagc tgaggttaca cctgttatta aattagatgg
     5761 tgaaccagtt aatgatggta aagttggccc aattacacgt caactacaag aaggatttga
     5821 aaagtatata gagtcacaca gtatttaaaa ttctttcatc atatttttag attaaattac
     5881 ttaaatttat caactttcgt aagtgaatta tgttataatt taaaacgaag cttaaaagta
     5941 ttgtttagaa aacacatatt agtaaaaagt gccattttgt ttctcatttt cagttgaaat
     6001 ataatgataa aaattataaa attttctatg agttacaaat atgatgtgca tcatatttta
     6061 ctaagtacgt aaatgttaca gttcataaat ttatgctaac catgaaaatg ggatagcaat
     6121 tattttgaaa actagagttt atcgcctaca aaagttacaa aaacatgtag ttttagacac
     6181 ggtaacattt tatgtcaagt aagtgactat agaatgtatg gtgtcaatga cagacattac
     6241 gatttaaatt tagtcgtgta atgtaggcat tagttgagga tttacaataa ttatctggac
     6301 ataatatttg taccagtatg gtgttataaa ttagaatata tggctcttga aaaacctatt
     6361 tctagagcca ttttctaatt gaaagtgagg tggacgagtt ggagcagttt tatcaattag
     6421 ggtggacact tgattctgca ggtggtgcat ctggcgaagc atatatggct gaacaagatg
     6481 gacaaaagtt gtttttaaaa cgaaattcaa atccatttat tgcggcatta tcagcagaag
     6541 gtattgtgcc caaattagta tggacgaagc gcatagaaac aggcgaggtt gttacagcac
     6601 aacattggaa aaatggccgt gaactatctt caaacgaaat gaagcaaaca agagttgcac
     6661 atttattaaa gaagatacac aattctagac ctttattaag tatgttaaag cgtatggaaa
     6721 tggaacctat tactcctgag attatgctta ataaaattaa tgcctcttta tcaagagaag
     6781 ttttaacaca tcatattgtg agaaaatcat taacctattt agaagagcat ataccgagtt
     6841 tagattcacg ttttttcact gttgtacatg gtgatgtgaa tcataataat tggttattat
     6901 ctgatcgaga tgaactattt ttagtcgatt gggaaggtgc aatgatcgca gacccggcaa
     6961 ttgatatcgg aatgctgctt tacaactatg ttccacaaca acaatggtct gaatggcttg
     7021 aaacatatgg tgttcaagag agcttgaatt taaataaacg tatgaaatgg tatacggtaa
     7081 ttcaatctat aggtcttgtg caatggtatg aagagcaaaa aagatataag gatatgaaca
     7141 cttggcttaa attcctaaat caagttatga atagtaacat gtttatttaa gaggagaata
     7201 aaatatgaga gttcgataca aaccatgggc tgaagattat ttaaaagatc atcctgaatt
     7261 ggttgacatg gacggtcaac atgcagggaa aatgactgaa tggtttgata aaacgcaacc
     7321 gatacacatt gaaattggtt cgggtatggg gcagtttatt acaacattag ctgctcaaaa
     7381 tcctcatatt aactatattt caatggaacg tgagaaaagt atcgtttata aagtattgga
     7441 taaagttaaa gagatgagtt taacaaattt aaagattatt tgtaatgatg ccattgaatt
     7501 aaatgaatat tttaaagacg gcgaagtttc gcgtatttat ttgaactttt cagatccatg
     7561 gccaaaaaat cgtcacgcaa aacgacgttt aacgtatcat acgtttttag cgttgtatca
     7621 acaaatctta aatgatgaag gagatttaca ttttaaaact gataacagag gactatttgc
     7681 ctacagttta gaaagtatgt ctcaatttgg aatgtatttt acaaaaataa atctaaattt
     7741 acatcaagaa gacgatggca gtaatattct aacagaatat gaaaagaaat tttcgaataa
     7801 agggtcacgt atttatcgta tggaagcgaa attccattca caaaaataaa gcacttatga
     7861 gggtatgtac acatacaatc ataagtgttt tttcgtacta taatggttct atagtaaaag
     7921 ggttctataa taaatcggac tgatagaaaa agtattttac ttttcatctg tccgattttt
     7981 tgattttgaa tataaaaaag cgcaattgcc tctgtaattt aaagtgacca aacctaaact
     8041 aaaggagaca attgcgccta tgaacaatgt tatactaaat acctttgatt taaaagaata
     8101 aagcttgcat tttacttgga aattttaatt attaatttaa aattacagtc cgatggttat
     8161 gatgcattta catataatat gattgcatta atctttataa gtagttagaa gtgtattagt
     8221 gtggggcgtt tcgtttttgt tcgcatttat gaatagtgat atgatgtaat aaacacatgc
     8281 taattgtaat aacgaataca gaaagaatag gtgatgttgt gaaaatcggg gatatatcta
     8341 ttcattatct aaatggcggc aatacaaaaa tggatggcgg tgcaatgttt ggtgttgttc
     8401 cgaagccgtt gtggtcaaag caatacaatg caaatgaacg aaatcaaatc aatttaccga
     8461 cacatccaat tttgattcaa acggctcaat ataatttgat tatagatgcg ggtattggta
     8521 atggtaaatt atctgaaaag caattacgta attttggagt agatgaagaa agtcatatta
     8581 ttactgattt ggcaaattat aatttaacgc caaaggatat tgattatgtg ctaatgacac
     8641 atatgcattt tgatcatgct gccggtttga ctgatcaagc gggacatgca atttttgaaa
     8701 atgcgattca tgttgtgcaa caagatgagt ggcatgagtt tattgcaact aatataagaa
     8761 gtaaatcaac ttactgggat aagaataaag gcgattatag taacaagttg attttattcg
     8821 aaaaacattt tgaaccggtt ccgggtatca agatgcaaca tagtggaggt catagctttg
     8881 gccacacgat tattacgatt gaaagtcaag gagataaagc agttcatatg ggtgatatat
     8941 tcccaactac tgcacataaa aatcctctat gggtaacggc atatgatgat tatcctatgc
     9001 aatcgattcg tgaaaaagaa cgcatgatac catattttat tcagcaacaa tattggttct
     9061 tgttttatca tgatgaaaac tactttgctg taaaatacag cgatgatggt gaaaacatag
     9121 atgcatatat tttacgtgaa acattagttg ataataacta aaataaagat gtattactaa
     9181 acaaattttc aaaaataaaa aattgagcca catccaatct tactaattag ggtgtggctc
     9241 atttttaagt tttacgatcc aaatcaaata tggataaaat tcgtattaac gctctacaat
     9301 gttaatgact tcaccagtat atgcatctgc ataaaaatca taatgaatat tttgaccatt
     9361 tttaatagtt gtaattccac cttgataaac taaacggtat ttatcagttt caggatgaac
     9421 gaatggttca taaacgatat atgagccttt gacattcata aaatatgatt ttacttcatt
     9481 aagtacttta acgggattat agcgtttacg attaatatat tggatgattg aaatggtaga
     9541 aataattaca atggctactg caattattgt tggaattata tatttcagtt tagtcatcat
     9601 ttatgctccc ttgatttcaa attcatatca ttagtttacc atattgaaga tgatataata
     9661 acttataagg agtgagaact tttatgaaca taaataaaaa agtaacatta caacgaattc
     9721 aaactttaac tgagcttcat ggggcaccag gttttgaaga agaagtaaaa aattatatga
     9781 ctcagcaaat ggcgccgtac gtagatgaat ttattgaaaa tcgtatgggt ggattttttg
     9841 gtgtgaaaaa atctaaaaat ccaaatgcaa aacgtgtaat gattgcagca catatggatg
     9901 aaatcggatt tatgattaca aatatcacta aaaatggaat gattcaattc acaaatttag
     9961 gtggtgttgc aaatgatatt tggcaaggac aacgcttagt aattaaaaat agaaatggcg
    10021 ataaaattat cggtgttgtt tctaatatac ctaaacattt tcgtactggt agtgaaggcg
    10081 caccggaaat taaagattta acattagata taggtgctca aaatgaagat gaggtgcgtg
    10141 agcgcggaat agatatagga gatacaattg tacctcacac gccattcaca cagttatctg
    10201 aacatcgata tagtgctaaa gcatgggata atcgttatgg ttgtgtcttg gcaattgaaa
    10261 tactagaatt attaaaagat atagaattag atgtagactt gtatgttggc gcaaatgttc
    10321 aagaagaggt tggattacga ggtgcgaaag catctgcaga gatgatagac ccagacgttg
    10381 catttgtagt cgattgttca cctgccaatg acgttaaagg aagccaacca ttatctggtg
    10441 aacttggtaa agggacgtta attcgcataa aagacggtac aatgatttta aagcctgtat
    10501 ttagagacta tttattaaag ttagtagaag cacatgacat tgaacatcaa tactatatgt
    10561 caccaggtgg aacagatggt ggagaaattc ataaagctaa tattggtatt ccgactgcag
    10621 ttattggtgt atgtgcacga tatattcata gtacagactc agtatttgat ataagagact
    10681 attttgcagc tagatcttta ctttcagaag ccatttgtaa tttagataat aatcaaatag
    10741 aaacattaca atataaataa tcgggtaata acaactatta tctctaaata gttatatata
    10801 ttcattaatt aaggagacat aaaaatgaaa caacttgaat cagaacaaca atttgaatct
    10861 ttaaaacaag gtgctacagt atttgaattc actgcaggct ggtgtccaga ttgtagagtg
    10921 atagaaccag atttaccgga attagaagcg agatatccta tgtttgactt cgtatcagta
    10981 gaccgtgata aatttatgga tatttgtatt gaaaatggta ttatgggtat tccaagtttt
    11041 ctagtatata aaaatggaga actgcttgga agttatattg gaaaagaacg aaaatcaatt
    11101 gaacagatag atgcattttt agctcaatac gtgtaattta gactagagaa aaacggggta
    11161 atactcgttt ttctctgtta ctatgtgttg atttattgta aactattata aggtgcgaaa
    11221 ttaggagtgt tacatatgaa tacctttcaa atgagagata aattaaagga acgtttaagc
    11281 catttagacg ttgattttaa atttaatcgt gaagaagaaa ctttgcgtat ttatcgaaca
    11341 gataataaca aaggtatcac gattaaactt aacgctatag tcgcaaaata tgaagataaa
    11401 aaagaaaaaa ttgtagatga aattgtttat tacgttgatg aagctattgc acaaatggca
    11461 gataaaaccc ttgagagtat atcatctagt caaattatgc ctgtcattag agcgactagc
    11521 tttgataaaa aaactaaaca aggtgttcct tttatctatg atgagcatac tgcagaaaca
    11581 gcagtttatt atgcagtcga tttagggaaa tcatatcgtc taattgacga aagcatgtta
    11641 gaggatttga agttaactga acaacaaata agagaaatgt ctctgtttaa tgttagaaaa
    11701 ttgtcaaatt catatacgac tgatgaagta aaaggtaata ttttttattt tattaactca
    11761 aatgacgggt atgatgcaag taggatacta aatactgcat ttttaaatga aattgaggca
    11821 caatgtcaag gcgaaatgct cgtagcagtg ccacaccaag atgtgttaat tattgcagat
    11881 atacgcaata aaacaggata tgatgtgatg gcacatttaa caatggaatt tttcactaaa
    11941 ggtctagttc caattacatc attatccttt ggatataaac agggtcatct tgaaccgata
    12001 tttattttag gtaaaaataa taaacaaaaa agagatccaa acgtgattca gcgtttagaa
    12061 gcaaatcgtc gtaaatttaa taaagataaa tagaaataat tggataagga gttttgtcat
    12121 aatgaattta ttttacaatc ctaaatatgt aggagatgtc gcatttttac aaattgaacc
    12181 agttgaaggt gaattaaact acaataaaaa aggtaatgtt gttgaaatta ctaatgaagg
    12241 taatgttgta ggttataata tttttgaaat ttcaaaagat ataacaattg aagaaaaagg
    12301 tcatattaaa ttaactgatg aacttgtaaa tgtattccaa aagcgtattt cagaagctgg
    12361 ttttgattat aaattaaatg ctgatctatc accgaaattt gtagttggct acgttgaaac
    12421 taaagacaaa catcctgatg cagataaatt aagtgtacta aatgtaaacg ttggaaatga
    12481 cacattacaa attgtatgtg gcgcgcctaa cgttgaagct ggacagaaag ttgttgttgc
    12541 taaagtaggt gcagtgatgc ctagcggtat ggtaattaaa gatgctgaat tacgtggtgt
    12601 tgcctcaagc ggtatgattt gttcaatgaa agaattgaat ttacctaatg cacctgaaga
    12661 aaaaggtatt atggtattaa atgacagcta tgaaattgga caggcatttt ttgaataatt
    12721 aaggaaggta gtgaaaatat gagctggttt gataaattat tcggcgaaga taatgattca
    12781 aatgatgact tgattcatag aaagaaaaaa agacgtcaag aatcacaaaa tatagataac
    12841 gatcatgact cattactgcc tcaaaataat gatatttata gtcgtccgag gggaaaattc
    12901 cgttttccta tgagcgtagc ttatgaaaat gaaaatgttg aacaatctgc agatactatt
    12961 tcagatgaaa aagaacaata ccatcgagac tatcgcaaac aaagccacga ttctcgttca
    13021 caaaaacgac atcgccgtag aagaaatcaa acaactgaag aacaaaatta tagtgaacaa
    13081 cgtgggaatt ctaaaatatc acagcaaagt ataaaatata aagatcattc acattaccat
    13141 acgaataagc caggtacata tgtttctgca attaatggta ttgagaagga aacgcacaag
    13201 tcaaaaacac ataatatgta ttctaataat acaaatcatc gtgctaaaga ttcaactcca
    13261 gattatcaca aagaaagttt caagacttca gaggtaccgt cagctatttt tggcacaatg
    13321 aaacctaaaa agttagaaaa tggtcgtatc cctgtaagta aaccttcaga aaaagttgag
    13381 tcagataaac aaaaatatga taaatatgta gctaagacgc aaacgtctca aaataaacaa
    13441 ttagaacaag aaaaacaaaa tgatagtgtt gtcaaacaag gaactgcatc taaatcatct
    13501 gatgaaaatg tatcatcaac aacaaaatca atgcctaatt attcaaaagt tgataatact
    13561 atcaaaattg aaaatattta tgcttcacaa attgttgaag aaattagacg tgaacgagaa
    13621 cgtaaagtgc ttcaaaagcg tcgatttaaa aaagcgttgc aacaaaagcg tgaagaacat
    13681 aaaaacgaag agcaagatgc aatacaacgt gcaattgatg aaatgtatgc taaacaagcg
    13741 gaacgctatg ttggtgatag ttcattaaat gatgatagtg acttaacaga taatagtaca
    13801 gatgctagtc agcttcatac aaatggcata gagaatgaaa ctgtatcaaa tgatgaaaat
    13861 aaacaagcgt caatacaaaa tgaagacact aatgacactc atgtagatga aagtccatac
    13921 aattatgagg aagttagttt gaatcaagta tcgacaacaa aacaattgtc agatgatgaa
    13981 gttacggttt cgaatgtaac gtctcaacat caatcagcac tacaacataa cgttgaagta
    14041 aatgataaag atgaactaaa aaatcaatcc agattaattg ctgattcaga agaagatgga
    14101 gcaacgaata aagaagaata ttcaggaagt caaatcgatg atgcagaatt ttatgaatta
    14161 aatgatacag aagcagatga ggatactact tcaaatatcg aagataatac caatagaaac
    14221 gcgtctgaaa tgcatgtaga cgctcctaaa acgcaagagc acgcagtaac tgaatctcaa
    14281 gttaataata tcgataaaac ggttgataat gaaattgaat tagcaccgcg tcataaaaaa
    14341 gatgaccaaa caaacttaaa tgtcaactca ttgaaaacga atgatgtgaa tgatggtcat
    14401 gttgtggaag attcaagcat gaatgaaata gaaaagcata acgcagaaat tacagaaaat
    14461 gtgcaaaacg aagcagctga aagtgaacaa aatgtcgaag agaaaactat tgaaaacgta
    14521 aatccaaaga aacagactga aaaggtttca actttaagta aaagaccatt taatgttgtc
    14581 atgacgccat ctgataaaaa gcgtatgatg gatcgtaaaa agcattcaaa agtcaatgtg
    14641 cctgaattaa agcctgtaca aagtaaacaa gctgtgagtg aaagcaagcc tgcgagtcaa
    14701 gccgcaccat catcaagatt tgattcacaa gagtcaaaca caaatgcata taaaacaaat
    14761 aatatgacat caaacaatgt tgagaacaat caacttattg gccatgcaga aacagaaaat
    14821 gattatcaaa atgcacaaca atattcagag cagaaacctt ctgctgattc aactcaaacg
    14881 gaaatatttg aagaaagcca agatgataat caattggaag atgagcaagt tgatcaatca
    14941 acttcgtctt cagtttcaga agtaagcgac ataactgaag aaagcgaaga aacaacacat
    15001 ccaaacaata ctagtggaca acaagataat gatgatcaac aaaaagattt acagccttca
    15061 ttttcaaatc aaaatgaaga tacagctaat gaaaatagac ctcggacgaa ccaacaagat
    15121 gttgcaacaa atcaagctgt acaaacatct aagccgatga ttcgtaaagg cccaaatatt
    15181 aaattgccaa gtgtttcatt actagaagaa ccacaagtta ttgagtcgga cgaggactgg
    15241 attacagata aaaagaaaga actgaatgac gcattatttt actttaatgt acctgcagaa
    15301 gtacaagatg taactgaagg tccaagtgtt acaagatttg aattatcagt tgaaaaaggt
    15361 gttaaagttt caagaattac ggcattacaa gatgacatta aaatggcatt ggcagcgaaa
    15421 gatattcgta tagaagcgcc tattccagga actagtcgtg ttggtattga agttccgaac
    15481 caaaatccaa cgacagtcaa cttacgttct attattgaat ctccaagttt taaaaatgct
    15541 gaatctaaat taacagttgc gatggggtat agaattaata atgaaccatt acttatggat
    15601 attgctaaaa cgccacacgc actaattgca ggtgcaactg gatcagggaa atcagtttgt
    15661 atcaatagta ttttgatgtc tttactatat aaaaatcacc ctgaggaatt aagattatta
    15721 cttatcgatc caaaaatggt tgaattagct ccttataatg gtttgccaca tttagttgca
    15781 ccggtaatta cagatgtcaa agcagctaca cagagtttaa aatgggccgt agaagaaatg
    15841 gaacgacgtt ataagttatt tgcacattac catgtacgca atataacagc atttaacaaa
    15901 aaagcaccat atgatgaaag aatgccaaaa attgtcattg taattgatga gttggctgat
    15961 ttaatgatga tggctccgca agaagttgag cagtctattg ctagaattgc tcaaaaagca
    16021 agagcatgtg gtattcatat gttagtagct acgcaaagac catctgtcaa tgtaattaca
    16081 ggtttaatta aagccaacat accaacaaga attgcattta tggtatcatc aagtgtagat
    16141 tcgagaacga tattagacag tggtggagca gaacgcttgt taggatatgg cgatatgtta
    16201 tatcttggta gcggtatgaa taaaccgatt agagttcaag gtacatttgt ttctgatgac
    16261 gaaattgatg atgttgttga ttttatcaaa caacaaagag aaccggacta tctatttgaa
    16321 gaaaaagaat tgttgaaaaa aacacaaaca caatcacaag atgaattatt tgatgatgtt
    16381 tgtgcattta tggttaatga aggacatatt tcaacatcat taatccaaag acatttccaa
    16441 attggctata atagagcagc aagaattatc gatcaattag agcaactcgg ttatgtttcg
    16501 agtgctaatg gttcaaaacc aagggatgtt tatgttacgg aagcagattt aaataaagaa
    16561 taattatgag taaggagttt tatataatga cacactatca ttttgtcgga attaaaggtt
    16621 ctggcatgag ttcattagca caaatcatgc atgatttagg acatgaagtt caaggatcgg
    16681 atattgagaa ctacgtattt acagaagttg ctcttagaaa taaggggata aaaatattac
    16741 catttgatgc taataacata aaagaagata tggtagttat acaaggtaat gcattcgcga
    16801 gtagccatga agaaatagta cgtgcacatc aattgaaatt agatgttgta agttataatg
    16861 attttttagg acagattatt gatcaatata cttcagtagc tgtaactggt gcacatggta
    16921 aaacttctac aacaggttta ttatcacatg ttatgaatgg tgataaaaag acttcatttt
    16981 taattggtga tggcacaggt atgggattgc ctgaaagtga ttatttcgct tttgaggcat
    17041 gtgaatatag acgtcacttt ttaagttata aacctgatta cgcaattatg acaaatattg
    17101 atttcgatca tcctgattat tttaaagata ttaatgatgt ttttgatgca ttccaagaaa
    17161 tggcacataa tgttaaaaaa ggtattattg cttggggtga tgatgaacat ctacgtaaaa
    17221 ttgaagcaga tgttccaatt tattactatg gatttaaaga ttcggatgac atttatgctc
    17281 aaaatattca aattacggat aaaggtactg cttttgatgt gtatgtggat ggtgagtttt
    17341 atgatcactt cctgtctcca caatatggtg accatacagt tttaaatgca ttagctgtaa
    17401 ttgcgattag ttatttagag aagctagatg ttacaaatat taaagaagca ttagaaacgt
    17461 ttggtggtgt taaacgtcgt ttcaatgaaa ctacaattgc aaatcaagtt attgtagatg
    17521 attatgcaca ccatccaaga gaaattagtg ctacaattga aacagcacga aagaaatatc
    17581 cacataaaga agttgttgca gtatttcaac cacacacttt ctctagaaca caagcatttt
    17641 taaatgaatt tgcagaaagt ttaagtaaag cagatcgtgt attcttatgt gaaatttttg
    17701 gatcaattag agaaaatact ggcgcattaa cgatacaaga tttaattgat aaaattgaag
    17761 gtgcatcgtt aattaatgaa gattctatta atgtattaga acaatttgat aatgctgttg
    17821 ttttatttat gggtgcaggt gatattcaaa aattacaaaa tgcatattta gataaattag
    17881 gcatgaaaaa tgcgttttaa tatgtttata atagagtagt atgggtattt attattaatg
    17941 acattattac atgttaatta ggaggcgttt ttaatggatt ggattttacc aattgctgga
    18001 attatcgctg cgattgcatt cttaatttta tgtatcggta tcgtagctgt attaaattct
    18061 gttaagaaaa acttagatta tgttgcaaaa acacttgacg gtgtagaagg tcaagttcaa
    18121 ggtattactc gtgaaacaac agatttactt cataaagtaa accgtttaac tgaggatatc
    18181 caaggtaaag tagatcgttt aaactcagtt gtagatgctg ttaaaggtat cggtgactca
    18241 gtacaaacgt taaacagctc tgtagatcgt gtaacaaatt caattacaca taatatttct
    18301 caaaatgaag ataaaatctc acaagttgtt caatggtcaa atgttgcaat ggaaattgca
    18361 gacaaatggc aaaatagaca ctaccgtcgt ggaagtgcaa attacaaagc taataatgta
    18421 gcaactgatg caaatcatag ctatacttct cgagtagata aataattaat aattaaacaa
    18481 atgcacttaa atgaggttgt tacttattta agtgcatttg ttataggagg atacacaaat
    18541 gacgcaacaa caaaataata aaagaacatt aaaaaataaa cacacttatc aaaatgaacc
    18601 attacccaac cgtaaagatt ttgttgttag ttttataact ggcgcgcttg ttggttcagc
    18661 tttaggtttg tattttaaaa ataaagttta tcaaaaagca gatgatttaa aagtcaaaga
    18721 acaagaactg tcgcaaaaat ttgaagaaag aaaaacgcaa cttgaagaaa cggttgcctt
    18781 tacaaaagaa cgtgttgaag gatttttaaa caaatctaaa aatgagcaag cagcattaaa
    18841 agcacaacaa gcagcaataa aagaagaag
//
LOCUS       DAMZLE010000038        18769 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMZLE010000038 DAMZLE010000000
VERSION     DAMZLE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.038"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(85..549)
                     /gene="bstA"
                     /locus_tag="POB92_001187"
     CDS             complement(85..549)
                     /gene="bstA"
                     /locus_tag="POB92_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDI6578696.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKILDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVTGKENIDLNIYRPLFGNGSSPDEWKGEVPSIGTILEGLQTLPER
                     ARKLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            complement(653..1291)
                     /gene="pcp"
                     /locus_tag="POB92_001188"
     CDS             complement(653..1291)
                     /gene="pcp"
                     /locus_tag="POB92_001188"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDI6578697.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(1443..2399)
                     /locus_tag="POB92_001189"
     CDS             complement(1443..2399)
                     /locus_tag="POB92_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI6578698.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVNINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            2643..3614
                     /locus_tag="POB92_001190"
     CDS             2643..3614
                     /locus_tag="POB92_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDI6578699.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNIQDIIEDFSTTYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVAPDFRTVTPIIQNISVANGIALSKDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPDTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(4094..4609)
                     /locus_tag="POB92_001191"
     CDS             complement(4094..4609)
                     /locus_tag="POB92_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDI6578700.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            4989..5837
                     /locus_tag="POB92_001192"
     CDS             4989..5837
                     /locus_tag="POB92_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDI6578701.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            5866..6420
                     /locus_tag="POB92_001193"
     CDS             5866..6420
                     /locus_tag="POB92_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDI6578702.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            6473..8185
                     /locus_tag="POB92_001194"
     CDS             6473..8185
                     /locus_tag="POB92_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578703.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQKINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIVALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            8182..9015
                     /locus_tag="POB92_001195"
     CDS             8182..9015
                     /locus_tag="POB92_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6578704.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISVSSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELI
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     Y"
     gene            9062..9559
                     /locus_tag="POB92_001196"
     CDS             9062..9559
                     /locus_tag="POB92_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6578705.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDKAMDDIINQGD
                     IVFYK"
     gene            9677..10801
                     /locus_tag="POB92_001197"
     CDS             9677..10801
                     /locus_tag="POB92_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI6578706.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            11105..11923
                     /locus_tag="POB92_001198"
     CDS             11105..11923
                     /locus_tag="POB92_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDI6578707.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            11941..12555
                     /locus_tag="POB92_001199"
     CDS             11941..12555
                     /locus_tag="POB92_001199"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDI6578708.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            12548..13798
                     /locus_tag="POB92_001200"
     CDS             12548..13798
                     /locus_tag="POB92_001200"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDI6578709.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVIGEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            13814..14827
                     /locus_tag="POB92_001201"
     CDS             13814..14827
                     /locus_tag="POB92_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDI6578710.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLLPEQIIAGNGSDELIQKLMLIMPKGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGMAYDVEMAPHILRMRTLSKAFGIAGLRLGVLISTAGTIQRIQKIEHPYPLN
                     VFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYGEPVMKGYVRYSIATAPQLNQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            14796..15374
                     /gene="hisB"
                     /locus_tag="POB92_001202"
     CDS             14796..15374
                     /gene="hisB"
                     /locus_tag="POB92_001202"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDI6578711.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            15371..15949
                     /gene="hisH"
                     /locus_tag="POB92_001203"
     CDS             15371..15949
                     /gene="hisH"
                     /locus_tag="POB92_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDI6578712.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            15942..16646
                     /locus_tag="POB92_001204"
     CDS             15942..16646
                     /locus_tag="POB92_001204"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDI6578713.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            16643..17401
                     /gene="hisF"
                     /locus_tag="POB92_001205"
     CDS             16643..17401
                     /gene="hisF"
                     /locus_tag="POB92_001205"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDI6578714.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            17398..18030
                     /locus_tag="POB92_001206"
     CDS             17398..18030
                     /locus_tag="POB92_001206"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDI6578715.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            18223..18402
                     /locus_tag="POB92_001207"
     CDS             18223..18402
                     /locus_tag="POB92_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578716.1"
                     /translation="MKSELSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT         6560 a         2779 c         3480 g         5950 t
ORIGIN      
        1 atctgtatgt aagcagacta aaagtggcct tttaaacaaa taaaaaaacc gttaacgtgt
       61 tgttaacggt taataagcta atatttattt taaatttttt aatatacgag acatttcttc
      121 aattttacca gcatgaagtg ggatatgaat ggcatttaat actaataact cttctaagtt
      181 attacaacca acaattggtt gtttcaattc aattgctaaa tcatcttcag ttagttttcg
      241 tgcacgttca ggtaatgttt gaagaccttc taaaatcgtt ccaatactcg gtacttcgcc
      301 cttccattca tctggagacg aaccatttcc gaataaaggt ctatatatat ttaaatcaat
      361 attctcttta ccagtaacag ctaaggctga ttcaaaaatt gttaatacat gcccaaattg
      421 ccaatgaatg gtattagaaa aaggcttatg ataatcatca agtatctttt caactttcca
      481 agtatcatac acatttacta aatatcctaa acccatatca atgacatcaa ataccgtttt
      541 tgtagtcatt attaattcct ccccccataa ataaaacata cttccgtaag tattacccta
      601 ttaacaaagt tgttagtcat ttaaggaatg tattttattg gtgtacttat aactattccg
      661 ttgtgcccag ggctaggtgt aaatcctcat cattagaaat agcttcaatg gctgcagtca
      721 aacctgcaac tatcttttct aatggcatag atggtgtatc gggtttacca atgacctgct
      781 ctggtatgta tggcacatga ataaatccga atcgtaggtg agggtaatgc ttatcttgta
      841 aataacctaa gtgataaagt gtgtgattac aaacaaatgt acctgcgcta tttgaaagtg
      901 ctccaggaag tccttgatta ataatacttt gagtcattgc tttaactggt aaatttgaaa
      961 aataagctgg cgcaccgtct aagtgaatgg cttgatcaat aggttgaaaa tcatcattat
     1021 ctggaatacg tgcatcatca atattaatgg cgacacgttc tggggtaatg gcatttctac
     1081 caccagcttg tcctatagct agtacaacat cataatgatt agatgccaac gttttattta
     1141 taatattatc tactttctta aaagaggttg gtagttttaa tttatcgatt gtatgtgtgc
     1201 caataatatc ttctagttga gtcacagctt cccatgaggg attgatattt tgattgtcaa
     1261 aaggcgcaaa ccctgttact aaaatgtgca ttttaagaca ccccgcagta ttaaaattta
     1321 atttagtata acacgcgcat aattaaaaac atatcaggtt atgtatgcgc ataaaaaaca
     1381 gccttgaaaa acctttataa atcggatttt caaagcgtgt tttgaaaggt atttaattaa
     1441 aactaagcat gttgatgtaa atcatcaaag tttgttaaac gttgttgcca ctcattatca
     1501 ctaatattat ttgcttgaac ataacgatta cgctcatgtt tagcacattc ataagagcat
     1561 gcacctaaat atttagtttc gttttcttca gaaactaata tttgtttatt acattctggg
     1621 ttagcgcaat taatataacg ttcacatggt ttgccatcaa accaatcctt accaataatt
     1681 gttttttcaa cttggttgat attaacactg atgcggtcat caaatacgta cattttaccg
     1741 tcccaatatt caccttttgt ttcaggatct ttaccatatg tagcaatacc gccatgaagt
     1801 tgtgctacat cttcgaaacc ttcttttaaa agccatccag aaaatttttc gcatcgaatt
     1861 ccaccagtac agtacgtaac cactttttta tctgcaaata acgctttatt ctctttaatc
     1921 cagtcaggca aatctctaaa acgtgtgata tttggacgaa ttgcacctcg gaaatgacct
     1981 aaatcaaatt cataatcatt acgtgcatca ataatgactg tgtcatcatc ttcaagagct
     2041 tttctaaatt ctacaggtga taaatattgg ccagttgtgt gtcttggatc gacgtcatct
     2101 tctaaatcta aagcaacgat ttcttttcga ggacgtacat gcattttctt aaaagcatgt
     2161 ccttcagctt catcaatttt aaacaccata tctttgaatc gttcatcggc atgcatatgt
     2221 gccatatatt gttcggtttc ttcttttgta ccagataatg tgccgttaat accttctgta
     2281 gaaacaagaa ttctaccttt taaatggtgt gctttacaaa aggctaagtg atcctgagca
     2341 aactgttcag ggtcatcaat cgtcgtatat ttataatata aaagaacttg atagttcata
     2401 ggttaatctc cttctaaaaa catgttataa gcatgttaat ttttaaaata aagcgtcaat
     2461 acgcatattt atctacgtcc atcattttaa cagagttgta ttgtcattca atatttctag
     2521 cttgaaatca gccactagac cgataaaata gataaatgaa accgttatca ttttatttga
     2581 tagttatctt gtttaaaatg aaaatgatag ttaaaacgaa tgaaatgaaa ggaatgatca
     2641 tgatgtcaca acaagattta cctacattat tttatagcgg gaagtccaat agtgctgttc
     2701 caattatatc tgaaagtgaa ttacaaacaa ttacagctga accatggctt gaaatttcca
     2761 aaaaaggatt gcagctagaa ggattgaact ttgatcggca gggacaactc tttttattgg
     2821 atgtattcga aggcaatatt ttcaaaatca atcctgaaac gaaggaaatc aaacgacctt
     2881 ttgtaagcca taaagcgaat cctgcagcaa tcaaaataca taaagatggc cgattattcg
     2941 tttgttattt aggagatttt aaatctacag gaggcatttt tgcagctaca gaaaatggtg
     3001 acaacataca agatattatt gaagattttt caacaactta ttgtattgat gacatggtat
     3061 ttgattctaa aggtggattt tattttacag attttagagg atactctacc aatccactag
     3121 ggggcgttta ttatgttgcg ccggacttta gaacagtgac gcctatcatt caaaatatta
     3181 gcgtagcaaa tggtattgct ttaagtaaag atgaaaaagt actatgggta acagaaacta
     3241 cagccaatcg attacatcgc attgcacttg aagatgatgg tgtgacgata caaccatttg
     3301 gagctaccat accgtactat tttacaggtc atgaaggacc agactcatgt tgtattgata
     3361 gtgacgataa tttatatgtg gcaatgtatg gtcaaggccg agtattagtt tttaataaaa
     3421 ggggttatcc aataggacaa atattgatac caggccgaga tgaagggcat atgttacgtt
     3481 ctacgcatcc gcaatttata cctgacacaa atcaactcat catttgttcc aatgatatag
     3541 aaatgggcgg aggatctatg ctttatacag ttaatggctt tgcaaaaggt catcaaagtt
     3601 ttcagtttca ataagaccta cctgaatgaa aggcgtctaa atataagttt aaatcttagc
     3661 gatttttaag ttagaagtta aatataattt gcttcaattt gtaaaatgaa gttttgtata
     3721 acgtaaatag gcaatattta atataagaaa gtataaagaa gaattttctt gattatttct
     3781 aatgaaatca atgccaattt aataaggagt tgtttatttt ggctatttat attttttgta
     3841 caatactagt tttagttgca gttggagcac ttttgacaaa tagaataagg gataaaaaag
     3901 ataaaaggct agatatttta tctagtgtat tacttttaat ttcatcaata tcattgttac
     3961 tttatggtgt tattatcaat taagttaaat atgattttta atttagaagt taaaaaatgc
     4021 caactatacc tagggatgat atagttggca tttcgtatat atatattaaa acaatgagga
     4081 ttgtgtaatg agattattct gagattgaga attcagctga tgcttcgaat ttaacatctt
     4141 tgcctagcat tacgccacca gtttcaagtg cttggttaaa gttaatgccg tagttttctc
     4201 tattgattgt accagtaaca ataacacctg ttacttgaga accatccatt ggatttttac
     4261 ttactccgtt gaattcaaca tcgaatgttt cttcgttagt gatgccttta attgttaaat
     4321 caccaacaac tttgctttca gatactgatt ttgtcacaaa tgtaatttta tcaaattcgt
     4381 cagtaccaaa gaaatcacca gattttaagt ggttatcacg tgcttcgttt ttagtgttaa
     4441 ttgagcttgg aataattgtt gcagtagctt tcaaagtact gaagtcatta atatctcctt
     4501 caacagctac atcaaattga tcaaatgaac ctttcacttt agaaaccatt aaatgtttaa
     4561 tttggaattc taaactactg tgtgcaccat caaaagtaaa gttagtcata atataaaacc
     4621 tccatatttt tatctcttag atatattttt tatacttgat atacatacaa taccacctaa
     4681 aaaacaaaga tgcaagcatt ttatttcgaa ttcgaatatt ttctgtgatg tatttaaaga
     4741 atgacctata aaacaccaca ctattgttat accctagtca attaattcaa acataaagaa
     4801 gtgattacaa gtccgtgaaa tttagttaat gagataaatc tgactattat tatcattttt
     4861 atagtcacta ctattgactt tgcatcaagt gactcgtact attttaatat aattctgaaa
     4921 attcaaaata cagaacatta aatatgtaga taatattttt ttgaagtttt aaaaaaagga
     4981 ggcattttat gggaaattat ttggtattgc aatcagattt tggacttagt gatggcgctg
     5041 taagtgcaat gtatggagtt gcatatactg taagtgatga tatacgtgtt gaaaacttaa
     5101 cgcatgatat accaccgtat gacatttggg tagcgtcata tcgtttatat caaactgtaa
     5161 aatactggcc taaaggcaca gtttttgtat cagtggtaga cccgggtgta ggtagtgata
     5221 ggcggagcat tgcttgctta acatattcag gtcattacat tattacacct gataatggtt
     5281 ctttaagtca tattaaacat tacgaaggta ttaaaaaagt tatcgaaatt gatgaagtga
     5341 aaagccgatt gcctcattcg gaagaaagtc atacttttca tggtagagat gtttatgcat
     5401 acaatggtgc gagattagct gcaaataaga tagcgtttga acgtctcggt caggcaatta
     5461 atgttgatag tattgaggca ttagaaataa gagaagcgac aaaaaatgag gattctgtta
     5521 caggaagcat tgatatttta gatatcagat ttgggtcatt atggacaaat attccattgg
     5581 cattcttaaa atccttggaa attgttcatg gtaataattt ggtggtaaca atttatcatc
     5641 aagataagaa agtctatcaa aatattatta aatttgcgcg atcgtttgca gatgttaatg
     5701 tgggtgaacc tcttgtgtat gttaactcgc tagtaaatat tggtgtagcg gtcaatcaag
     5761 actcattttc agatttatat catattggta ctggaaatga ttggacgatt catttatcta
     5821 aagcaccatc aatttagtga attttttatg taaggagtaa tgaagatgaa aaaacaagat
     5881 atttcagtta aaactgttgt tgcgattggt ataggagcag cggtatttgt gattttaggg
     5941 cgttttgttg taataccaac aggttttcct aatacgaata tagaaacatc ttatgcattt
     6001 ttagcattaa tatctgcaat atttgggcct tttgctggtt taatgactgg attagttgga
     6061 cacgctatta aagatttcac gacatacggt agtgcttggt ggagttgggt tatttgttca
     6121 ggaattatag gctgtttata tggatggatt ggcctaaaat taaatctttc ttcaggccga
     6181 ttttctagga aatcgatgat ttattttaat atcggtcaaa ttattgcgaa tattatttgc
     6241 tgggcactta ttgcaccaac attagatatt ttgatttata acgaaccggc taacaaggtt
     6301 tatacacaag gtgttatctc tgcagtatta aatattattt cagttggcat tattgggaca
     6361 atattattaa aagcatatgc ttcatctcaa ataaaaaaag gtagtttacg taaagaataa
     6421 tcattttgtt gaatcagata tgtaaatgaa tgtagaaagg taatgatata tcatgactga
     6481 accaattatc tcgttcaaag actttagttt tcaatatcat agtcaagcaa cacctacatt
     6541 acagaaaata aatgttgata tttatccagg agaaaaagta ttagtagttg gtgcttcggg
     6601 tagtggtaaa tcgacttttg caaattgcat aaacggatta attccattta aaactaaagg
     6661 taacataact ggggaactat atataaataa tcaagatgca accgttagtt gtttacatga
     6721 tagatctaat gttgttggta cagttttaca agatacagat ggacagttca taggcttaac
     6781 agcagctgaa gatatggcct ttttattaga aaataattgt gttgaacaag atgatatgaa
     6841 gaaaaatgta agttattggg ctgaaaaagt tggcatgata gaacatttaa atcaccgacc
     6901 gcaagattta tctggaggtc aaaaacaacg cgtttcatta ggtggtatat taatccatcg
     6961 tacgcctatt ttaatattgg atgagccgct ggccaattta gatcctgcga caggacatga
     7021 aacgctgaga ttgttaaaca atattcatga agaaacaaag tcaacgatga ttattgtcga
     7081 acatcgatta gaagaatcat tagatgatac gtttgataga gttcttcttt ttaaagatgg
     7141 aaaaatcatc gctaatacaa cgcctagtga tttgctaaaa tcatctaagc ttaaagaagc
     7201 aggcatacgt gaaccattat actgtacagc gcttaaatat gcagaagtag atgtcgaatc
     7261 cattgataac ttagcgaatt tacgtgacgt ttgtatgagt gaacatgtta aatttaaagt
     7321 gaaaaaatgg atagatgaaa catctgcaaa taatgataat aagtacaaat ctgaaccgct
     7381 attagaatta aatgaggtat gtgttcaata tagtgactat agtaatagtg tacttaataa
     7441 tgttcaatta aatgtttata gaagagaaat gcttagtata gttggtcaca atggtgccgg
     7501 caaatcaaca cttgctaaag caatttgtgg atttttagat ataacaggta atatccaatt
     7561 ttgtaatagg ggatttaatc aattgtcaat tagcgaacga tctgaattcg taggttatgt
     7621 gatgcaaaat ccaaatcata tgatttccga aaaaatgatt tacgatgaag tagcattagg
     7681 gttaagagca cgaggtatga aagaatcaga tataaagata cgggtagaga atgtgctcaa
     7741 aatatgcgga ctttatgcat ttcggaattg gccaattgta gctttaagct atggtcaaaa
     7801 aaagagggtc actatagcat ctgttttagt cttaaatccg gaaataatca tattggatga
     7861 accgactgct ggtcaagatt tctatcatta taatgagata atgtcatttt taattgagct
     7921 aaacagacaa gggaagacga ttattatgat tacgcatgat atgcatttat tgtctgagta
     7981 tagttcaaga acagttgtat tatcaaaagg tcaagtcgtt gctgatacca cgccagtatt
     8041 ggttttaaat gataaaaaaa tctgtgagat tgcatcattg agacaaacat cgctatttga
     8101 aatggccgaa tatataggga ttagcgagcc acagaaatta gtacaattat ttattaacca
     8161 tgataggaag gtgagacgcc aatgaatcaa tataatacta taggttttca cccgggaaat
     8221 agtcgtattc atcaattaaa tgcgactgtt aaacttttat tcttattagt tgtttctatt
     8281 tctgcaatgg tgacttatga cacaagatat ttaattttaa ttagtgtttc atctatttta
     8341 ttgttcaaat atgctcatat tgaatggaaa caagttcgtt ttgttgttaa attcattctg
     8401 tttttcacaa tattaaatat tattgccgta tacatatttg atcctgaata tggtgtgaag
     8461 atttataatc agcgtacaga gttaatcaat ggtattggtc gatttacgct aacatcacag
     8521 gaattattct atctttttaa tctaatatta aaatatatta gtacagttcc tttagcgtta
     8581 atatttttat tcacaacgaa tccgagtcat tttgctgcaa gtttaaatca gctaggtgtg
     8641 aattataaaa tcagttacgc agtctcacta gcattaaggt atattccaga tattcaagaa
     8701 acatatttta atatttcaca agcacaacaa gcaagaggat atgataattc gaaaaaggcg
     8761 aaatttacta gccgtgttaa aggtatcaaa cgcattgtgt tacctttaat attttctagt
     8821 atcgaaagaa ttgacactat tagtactgct atggagttaa gacaattcgg acagtataaa
     8881 aggagaacct ggtacgtcaa aaaacaatta aaaaaagatg attatgttgt tttgtgtttg
     8941 acgttaatac ttctgatgtt agtagttaca ttattctttt taaataatag tcgatatttc
     9001 aacccgtggt attagcattc atataaatag tctttaaata gaaataggag ggagacattt
     9061 aatgataaat actgaaagat taaatttaat gattccaagt tcctcgcatt taattgaact
     9121 ttataatatt tgtagtcatc cacaagcaaa tatatacact cccaaaggtt tacataattc
     9181 caaattagac acacaacggt ggattgaaaa atggcgaaac cattggcaac aatatcaatt
     9241 tggttacttt gtattggtaa aaaaaataga ttgtagtgtt attggtattt gtggatatga
     9301 atatcgacaa ttaaagcaag aaacagtatt aaatttattt tataaattac atccaagttt
     9361 tgaaggacaa gggtacgcat gtgaggctat tacagcaatc acaaattttg tgaattatat
     9421 cgatcaagaa acagtaaaag ttatcaggac aaataagtgt aaccaacgtt caataaattt
     9481 agcagaaagg cttaaattca agcgagacaa agctatggac gacattatca atcaaggaga
     9541 tattgtgttt tataaataaa atactatgac attatctaaa aaataaaatt aaaatcccaa
     9601 cagtttctta aaaagttatg agccacataa taaaaatgtt ataattgtgc ataataaaac
     9661 atagggagaa tgtttaatgc ataaaaaata tttattgcta ttttttctgt tattatcagt
     9721 tgcattgcta tatagctgtt ccaagcaaaa tgagaaagct caaaataaat ctgttaatcc
     9781 aaatgagtat gcaaagcata aatcgaaaat ggatgaacat tgggaaacat atcacggcca
     9841 agtttatcat gtgttttatc acccattaat tacagaccca aaagttgcgt ttacaggaga
     9901 tacacaccaa gctaagggca acaatgattg gatgattact gtaagtgaat ttaaaaaatc
     9961 attgaacgaa ttatataaaa ataattacat catcgttaat ccacatgatg tatacgactt
    10021 atcatctcat catgtaaaag ccaaaaaaat aaaattgccg aaaggtaaaa agccactaat
    10081 catctcaatt gatgatatga attactatag ttacatgaga caccatggtt atgctgaccg
    10141 tttagtactc gataaaaata aacatgttgt ttctgaaact acagacaaaa aaggtcatgc
    10201 tacttattct gatgacaatg atatcgtgcc aattttaaat aaatttgtaa aagagcatcc
    10261 agatttttct ttaaatggtg aaaaaggtgt tgttgcttta accggttatg agggcgtatt
    10321 aggttatcgc acgaatgaat taacgagtaa agattataca aagaataaaa aggcagctga
    10381 ggaagttgtt cgcgcaatga aacgggatgg atggtcattt gcgagtcatt catatggtca
    10441 cattaatttt gaaaaaacat ctttagaagg tattaaacgt gatactaaaa gatggaaaga
    10501 tgaagttgaa ccaattgtag gtaaaacaga tatgtttgta tttccacatg gcgcacagga
    10561 tagacataca caagcgtatg attatttggt agatgaagca gagtttaagt ttattgcagg
    10621 tgtcggtcca aacaatttta cagatatatc agcaacgaac gtttaccaag atagggttgc
    10681 gattgatggt ctaaacttat ttgaatttaa atataaactc aaaccgtttt ttaatcctga
    10741 aaatgtatat agtaaacaag atagacaatt ctttaaaggt aatcgggatt atgaagaata
    10801 gttaaattta ataaaaagat agaaagaaaa taactttaca taataaggca agcacaatat
    10861 gtgcaagaag tacaacttca atataaaaat cttggtttag catttttata ggcacaaatg
    10921 tcttgccttt ttcttttgta aaaatttaaa aacaatacac cgaattttct gaaaaaggtt
    10981 gtaatgtatt gctaaaaatg ataagttatt aatcatatta cactttagtg tggtagagaa
    11041 gtaaagtaaa caacgaaaat accaagtgat tttaaataca gtattgaaag taaagggtgg
    11101 tcgagtgaat aattcagaac aattaattgc gttaaaagaa tcggaaacag catttttaaa
    11161 atattttaat aaagcggatt atgagttagt tgattttagt gtggttgaaa agttggactg
    11221 gaaacaattg aaccatgagg atttgcagca gatgggtgag cgaaattttt ggcaacatga
    11281 gcatcaaatt tatgcacttc gaaatgattt tacagatcaa ttattgcgtt attactcaat
    11341 gtatccaact gcagcaacaa aagtggcata cacaggttta atcattcgga ataatgaagc
    11401 ggctgtacaa gtgggtttgg aaaactatgc accatcatta gcgaatgtac aacagagctt
    11461 aaagctattt attcagttta ttcaacagca gttgagagac aacgtgcatt ttgttgtatt
    11521 gggacattat caattgcttg atgccttact cgataaatcg ttacaaacac cagacatact
    11581 atcaatgatt gaggaacgta atttatcggg actggttaca tatttgtcga ctgaacatcc
    11641 aatcgtgcaa atcttgaaag agaatacgca acaacaatta aatgtattgg aacactatat
    11701 tccaaatgac catccagcac tcgttgaatt aaaaatatgg gaacgttggt tacatacaca
    11761 aggttacaaa gacatccatt tagatattac tgcgcagcca cctagatcct attacacagg
    11821 cttatttatt caatgtcatt ttgctgaaaa tgaatctcga gttttaacag gtggttatta
    11881 caaaggaagc atcgaagggt ttggattagg attaacactt taagtaaggg agtatgcaca
    11941 atgttaagaa tcgccatagc caaaggacgt ctaatggata gtttaattaa ctatttagat
    12001 gtaattgaat atacgacatt atcagaaaca ttaaaaaata gagaacgcca attattatta
    12061 agtgtagata atattgaatg cattttagta aaaggaagtg acgtgccaat ctatgtggaa
    12121 caaggaatgg ccgacatagg cattgttggt agcgacatat tagatgagcg ccaatataat
    12181 gttaataatt tgttgaatat gccttttgga gcatgtcatt ttgcggttgc agcgaaacct
    12241 gaaacgacca attatcgtaa aatcgcaacg agttatgttc atactgctga aacatatttt
    12301 aaatcaaaag gtattgatgt cgaattgatt aaattgaatg gttctgttga attagcttgt
    12361 gtcgtagata tggtagacgg aattgtcgac atcgttcaaa caggtactac gctaaaagcg
    12421 aacggactgg ttgaaaagca acatattagt gatatcaatg caagattaat aactaataaa
    12481 gcagcttatt ttaaaaaatc acaattaata gagcaattta ttcgctcttt ggaggtgtct
    12541 attgccaatg cttaatgcac aacaattttt aaatcaattt tcattagaag caccattaga
    12601 tgagtcattg tatccaatta ttcgcgatat ttgtcaggaa gttaaagttc atggagataa
    12661 ggctttgaaa atgtataatc taacattcga tcatacgaaa acagatcatt tagaaattag
    12721 tcatgaacaa attaaagcag catttgacac attagatgaa aaaacaaaac aagcattaca
    12781 acaaagttat gaaagaatta aagcatatca agaaagtatt aagcagacga atcaacagtt
    12841 agaagaatca gtggagtgtt atgaaatata ccatccacta gaaagtgtgg gtatttatgt
    12901 gcctggtggc aaagcaagtt atccatcaac ggttctaatg acagcgactt tagcacaagt
    12961 agcgggtgta gaaaatattg ttgttgtgac accacctcaa cctaacggag tatcccaaga
    13021 ggtattagct gcatgttata ttacgcaagt taatcaagtg tttcaagttg gtggtgctca
    13081 aagtattgct gcattgactt atggaacaga aacgatacct aaagttgata agattgtagg
    13141 tccaggtaac caatttgttg catatgccaa aaaatattta tttggacagg taggtattga
    13201 tcaaattgca ggaccaacag aaatagcact gattattgac gacaccgcag atttagatgc
    13261 catcgtatat gatgtatttg cgcaagcaga acatgatgaa ttagcacgta catatgtcat
    13321 tggtgaagat gcgcaagtcc ttaaagattt agaatcacgt attgctaaag cattgcctaa
    13381 tgtggacaga tacgacattg tttctaaaag tatcgctaat caacactacc ttatccatgc
    13441 tagtaatttt gatgaagcat gccatgtcat gaatacaatc gcgcctgaac atgcgtcgat
    13501 tcaaacagta aatcctcaac catatattga aaaagtgaaa tatgtgggtg cattgtttat
    13561 tggacattat tcgccagagg tcataggaga ttacgttgca ggtccaagtc atgtattacc
    13621 tacaaataga acagctagat ttaccaatgg gttatcggtc aatgatttct taacacggaa
    13681 cacggtcatc catttatcaa aagatacgtt tgaacaaatt gctgattcag cacaacatat
    13741 tgctcatgtt gaagcattat acaatcacca gcagtctatt ttaatacgtc agtcttaggg
    13801 gagtgtaatt gaaatgattt atattgataa aaatgaaagt ccagttacgc cgttggatga
    13861 aaaaacaatg acgtctatta ttagtgcaac gccatataat ttatatcctg atgcagcata
    13921 tgaacaattc aaggaagctt atgctaagtt ttacggatta ttgcctgaac aaattattgc
    13981 aggaaatggc tctgatgaat tgattcaaaa gttaatgcta atcatgccaa aaggtccggc
    14041 attaacgcta aatcctgatt tttttatgta tcaagcatat gcggcacaag taaatcgtga
    14101 aattgcattt gtagatgcag gatcagattt aacgtttgat ttggaaacca ttttaacgaa
    14161 aatcgatgaa gtacaaccat cattttttat tatgagtaat ccacataacc cttcaggcaa
    14221 gcaatttgat acggcatttt taacagctat tgcagataag atgaaagcat taaacggata
    14281 cttcgtcatt gatgaagcat atttagatta tggcatggca tatgacgttg aaatggcacc
    14341 acacatttta agaatgcgca cattatcaaa ggcgtttgga atagctggct taagattagg
    14401 tgtcttaatt agtactgctg gaacgataca gcgtattcaa aaaatagaac atccatatcc
    14461 attaaatgta tttacgctaa atattgcgac ttatattttt agacatagag aagagacaag
    14521 agtattttta acgatgcaac gacagttagc tgagcagtta aaacaaatat ttgatacaca
    14581 tgttgcagat aaaatgtcag tattcccatc aaatgccaat tttgtactta ctaaaggttc
    14641 agcagcgcaa caattaggac aatacgtata tgaacaagga tttaaacctc gcttttatgg
    14701 tgagccggta atgaagggct atgtaagata ctcaattgca acagcaccac agttaaacca
    14761 attagaagaa attgttaaag aatggagcgc aaaatatgat ttatcaaaaa caacgaaaca
    14821 cagctgaaac acaactaaat atttcaatat cagatgacca gtcaccatcg catattaata
    14881 caggtgtggg ctttttaaat catatgttaa cattgtttac atttcatagc ggtctgtcat
    14941 taaacattga ggcacaaggt gatattgacg tagatgatca ccacgtaact gaagatatcg
    15001 gcattgtcat tggccaattg ttacttgaaa tgattaaaga taaaaagcat ttcgttcgtt
    15061 atggaacgat gtacattcca atggatgaaa cattagcacg tgtcgttgtg gatataagtg
    15121 ggcgcccata cctatcattc aatgcatcat taagtaaaga aaaagttggt acgtttgata
    15181 cggagttagt agaagaattt tttagagcgg tcgtaatcaa tgcaagatta acaacgcata
    15241 ttgatttaat tcgtggaggt aatacacacc atgaaattga agctatattc aaagcgtttt
    15301 cccgtgcatt aggcatagcg ctaactgcaa ctgatgatca gcgtgtgccg tcatcgaaag
    15361 gtgtgattga atgattgtca tcgttgatta tggattaggg aatattagta atgtaaaacg
    15421 cgctattgaa catttagggt atgaggtggt tgtctcaaat acctcaaaaa taatcgatca
    15481 agcagaaaca atcatattgc ccggtgtcgg ccattttaaa gatgcgatgt cagagataaa
    15541 gcgattaaat ctcaatgcaa tattggctaa gaatactgat aagaagatga ttggtatttg
    15601 tttaggcatg caattaatgt atgagcatag tgatgaaggc gatgcatctg gattagggtt
    15661 tatcccagga aatatttcgc gtatccaaac agaataccca gtgccacact taggctggaa
    15721 taatttagtg agtaagcacc ctatgttaaa tcaagatgtt tatttcgtac attcttatca
    15781 agcgccgatg tcagaaaatg taattgcata tgcgcagtat ggggctgata ttccggcaat
    15841 tgttcaattt aacaattata ttggtattca attccatcct gaaaaaagcg gtacatatgg
    15901 gttacaaatt ttgcgtcagg caatacaagg gggatttata aatgattgaa ttatggccag
    15961 cgattgattt gattgggtca acaagtgtga ggttaacaga gggtaaatat gatagtgaag
    16021 aaaaaatgtc acgctcggct gaagaaagta ttgcttacta tagtcaattt gaatgtgtga
    16081 atcgtattca tatcgtcgac ttgataggtg ctaaggcaca gcatgcccga gagtttgatt
    16141 atattaagtc attgaggaga ttaacaacca aagatattga agtaggtggt ggcattcgta
    16201 cgaagtcaca aatcatggac tactttgccg cagggattaa ttattgcata gttggaacga
    16261 aaggtattca agatactgat tggcttaaag agatggcaca tacatttcca ggtcgcattt
    16321 atttatctgt tgatgcctat ggagaagata ttaaagtgaa cggatgggaa gaggacacag
    16381 agttaaattt atttagtttt gtgagacggt tatcggatat acctcttggc ggcattatat
    16441 atactgatat tgctaaagat ggcaaaatgt ccggacctaa ctttgaatta actggtcaat
    16501 tagtaaaggc aacgacgatt cctgtcattg cttccggtgg tattagacat cagcaagata
    16561 ttcaacgatt agcgtcgcta aatgttcacg ctgctattat aggaaaggct gcacatcaag
    16621 catctttttg ggaggggcta aaatgattaa aaaacgtatc attccatgtt tagatgtcaa
    16681 agatggtcgt gtcgttaaag ggattcaatt taaaggatta agggatattg ggaatcctgt
    16741 tgatttagca atgtattaca atgaagcggg tgctgatgaa ttagtatttt tagacatctc
    16801 taagacggaa gagggtcata gcttaatgct agaagtgatt gaacagacag cgtcacgctt
    16861 gtttatccct cttactgtag ggggtgggat tcaaagtctc gatgatatta cccaattgct
    16921 aaatcatggt gcagataaag tatcattaaa ttcaagtgct ttaaaaaatc cacagctcat
    16981 taaacaagcg agtgataaat tcggtagaca atgcatctgc atagcaattg atagctatta
    17041 tgatcctgaa agaaaagcac attattgttg tacgactggt ggtaaaaaaa tgacaaatat
    17101 taaagtatat gactgggtac agcaagtaga acagttaggt gcaggtgagc tcctcgttac
    17161 aagtatggga catgatggta tgaaacaagg ctttgatatt gaacacctag caaatattaa
    17221 gtctcttgta aatattccaa tcattgcttc tggtggtggt ggcaatgcac aacactttgt
    17281 agaattattt gatcagacgg atgtttctgc aggtttagct gcaagtatat tacatgatcg
    17341 agaaacgacg gttcaatcta ttaaagaagt gatacggcaa ggaggtatag cagtaagatg
    17401 accaattaca aaattgattt tagcaaaggt ttagtgccag caattttaca agataatcaa
    17461 acaaaacaag tattgatgtt gggttatatg aatcaagaag cttttgataa aacgatagaa
    17521 gatggtgtag tatgtttcta ttcgcggtcg aaacaacgtc tatggacaaa aggtgaaaca
    17581 tctggtcata cgcaacgtgt taaagatatt catgtagatt gcgacaatga tactatttta
    17641 attgatgtca taccaaatgg accaacatgt catacaggca gtcaaagttg tttcaacaca
    17701 gaagttccat tttcagtgca aacattagcg cagacagttc aagatagtgc ccaatccaat
    17761 aatgaaaagt catatacaaa atatttatta acagaaggta tagaaaagat tacgaaaaaa
    17821 tatggtgaag aagcttttga agtcgtaatt gaagcaatta aaggtgacaa aaaagcattt
    17881 gtaagtgaag tagcagatga actttatcat ttatttgtct tgatgcatgc gctaggcgtc
    17941 gatttttcag aaattgaggc agaattagcg cgtagacatc ataagcgcaa taactttaaa
    18001 ggcgaacgac aaaatatcga acagtggtaa aacaagtatg ggctgagata tagagaaacg
    18061 gatcatggct tatacactta caaatattgt ggaaaacgtg acattttcaa gtttaaaata
    18121 cgacaccaac atattttaac tatgaatgct gtgatggtac taaagttgca aacgcgttat
    18181 agataagtag tggataatca caatacgaaa ccaaaaataa ttatgaaaag tgaattgagc
    18241 aactcaggaa tagatgtcac tgttaaagat gttgaaaagt atatgaatcg atataatgaa
    18301 gttatgaagg gaaaaaatgg cgaaaaagct aaagagttat gtttgtcgtt actacctatt
    18361 aatatcatag ttgtctttac attctttgta tttatactat aaataaaaat atatctagcc
    18421 tgaaatagaa atgtcatagc ctatttaaaa gacaatctcc attagaacta agatgtgcat
    18481 cccgaaagtt agactaaaaa actaacttta tgggatgtat ttttatgcta atcatcataa
    18541 attcgagatt aagttaaagg tagttcaaga gtaattaaac aataaattaa aaatagtagg
    18601 atacttactt tgagggaaga aaattaactg tatatattta gtttaggaac aagtattacg
    18661 gtttatcctg atacaattat tgtggatggg atgatatttt taggtttaaa atacgacacc
    18721 agcaaacata ataactgtaa tagctcataa atctccccat atagctaat
//
LOCUS       DAMZLE010000039        18448 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMZLE010000039 DAMZLE010000000
VERSION     DAMZLE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.039"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            242..769
                     /gene="pyrR"
                     /locus_tag="POB92_001208"
     CDS             242..769
                     /gene="pyrR"
                     /locus_tag="POB92_001208"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDI6578717.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            987..2294
                     /locus_tag="POB92_001209"
     CDS             987..2294
                     /locus_tag="POB92_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI6578718.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            2322..3203
                     /locus_tag="POB92_001210"
     CDS             2322..3203
                     /locus_tag="POB92_001210"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDI6578719.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            3221..4495
                     /locus_tag="POB92_001211"
     CDS             3221..4495
                     /locus_tag="POB92_001211"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI6578720.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            4497..5597
                     /gene="carA"
                     /locus_tag="POB92_001212"
     CDS             4497..5597
                     /gene="carA"
                     /locus_tag="POB92_001212"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI6578721.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            5590..8763
                     /gene="carB"
                     /locus_tag="POB92_001213"
     CDS             5590..8763
                     /gene="carB"
                     /locus_tag="POB92_001213"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI6578722.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            8873..9565
                     /gene="pyrF"
                     /locus_tag="POB92_001214"
     CDS             8873..9565
                     /gene="pyrF"
                     /locus_tag="POB92_001214"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI6578723.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            9565..10176
                     /locus_tag="POB92_001215"
     CDS             9565..10176
                     /locus_tag="POB92_001215"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI6578724.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYSELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            10206..10418
                     /locus_tag="POB92_001216"
     CDS             10206..10418
                     /locus_tag="POB92_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578725.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            10855..11256
                     /locus_tag="POB92_001217"
     CDS             10855..11256
                     /locus_tag="POB92_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6578726.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(11519..13216)
                     /locus_tag="POB92_001218"
     CDS             complement(11519..13216)
                     /locus_tag="POB92_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDI6578727.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIKSIKQIGNDRRIEIE
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            13491..14114
                     /gene="gmk"
                     /locus_tag="POB92_001219"
     CDS             13491..14114
                     /gene="gmk"
                     /locus_tag="POB92_001219"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI6578728.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            14114..14332
                     /locus_tag="POB92_001220"
     CDS             14114..14332
                     /locus_tag="POB92_001220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI6578729.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGTIRPVISSDYYGKE"
     gene            14548..15747
                     /gene="coaBC"
                     /locus_tag="POB92_001221"
     CDS             14548..15747
                     /gene="coaBC"
                     /locus_tag="POB92_001221"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDI6578730.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            15747..18155
                     /gene="priA"
                     /locus_tag="POB92_001222"
     CDS             15747..18155
                     /gene="priA"
                     /locus_tag="POB92_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI6578731.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         6523 a         2710 c         3503 g         5712 t
ORIGIN      
        1 agtattatcc ttataacagt ttaagtacaa cataaagagt ctttaattga gcgtccagag
       61 agacgtcaaa gacatgaaaa tatagatata tactaaaatg atcataacac tttcacatat
      121 gcaagaataa gtagtaatac tacgattctt ctatgtaaat aggctgattg tatatactaa
      181 acctcatgtc gatattaaag acatgaggtt ttttgtttct aaattttgaa agggtgagaa
      241 tatgtctgaa cgtatcataa tggatgatgc cgcaatacaa cgtacagtga cgagaatcgc
      301 tcacgaaatt ttggagtata acaaaggtac tgataattta attcttttag gtatcaaaac
      361 aagaggtgaa tatttagcga atcgtataca agataaaatt catcaaattg agcaacaacg
      421 tatacctact ggaacaattg atattacata ctttagagat gatatagagc acatgtcatc
      481 acttacgaca aaagacgcaa tagacatcga cacagatatt acagataaag tagtcatcat
      541 tattgatgat gtgctgtata ctggtcgaac ggttcgtgct tcacttgatg ctattttgct
      601 aaatgctaga cctattaaaa ttggtttagc tgctttggtt gatcgaggac atcgtgagtt
      661 accaattcga gcagattttg ttggtaaaaa tatacctact tctaaagagg aaacggtaag
      721 tgtctattta gaagaaatgg atcaaagaaa tgcagttata attaaataat acccctttta
      781 attcagtacg agagactgaa aaaggtgggg gagttgaatg ataaataagt tttcatatgc
      841 ttgtttatct aataataccc tcatgttcga caactataag tcaatttcta tggacgaaaa
      901 tgacttgtag caacttgtca cactttttgt ctctttacat atactgatgt aaagagattt
      961 ttttattatc ggaggaaatg aatattatgc aaaatgatga aatgtttgaa cgaacagtaa
     1021 aacctgtact agatgtaaat gaaaaaccac aaccagcgca atgggcattt ttaagcttac
     1081 aacatttatt tgcgatgttt ggcgcaacag tactagtacc attcttaaca ggattaccaa
     1141 tatccgcagc gttactagct tcgggaatcg gtacattact ttatatctta ataacgaagg
     1201 cgcaaatacc agcatacttg ggatctagct ttgcatttat cacgccaatt atcacgggat
     1261 taagtacgca tagcttagga gacatgcttg tagcattatt catgagtggt gttatgtacg
     1321 tcatcatcgg gattctaatc aaattaagtg ggacagcatg gttaatgaaa ttattaccac
     1381 cagttgttgt tggaccagtc atcatggtaa ttggtttaag cttagcgcct actgcagtca
     1441 atatggcaat gtatgaaaat cctggagata tgaaaggtta caatatcagt ttcttaattg
     1501 ttgcaatgat aaccttgctt gtaacaatag tcgttcaagg attttttaaa ggattcctat
     1561 ctttaattcc agtacttgta ggtattatcg taggttatgt ggtagcgatt tttatgggga
     1621 ttgtgaaatt tgatgcaatt atgtcagcaa aatggataga tttccctcat atttatctgc
     1681 catttaaaga ttatgtacct tcatttcact taggacttgt actggtaatg attccgattg
     1741 tgtttgtaac agtaagtgaa catattgggc accaaatggt attgaataaa atcgtaggta
     1801 gaaacttctt tgaaaagcca ggacttgata aatcaatcat tggtgatggt gtttctacaa
     1861 tgtttgccag tattattggt ggaccaccaa gtacaacata cggtgaaaat atcggtgtat
     1921 tagcgattac cagaatatac agtatttacg tcattggtgg tgcagcagtt atagcaattg
     1981 ttttagcatt cattggtaag ttcactgcat taatttcttc tatacctaca ccagttatgg
     2041 gaggagtatc tatattactt ttcggtatta ttgcagcaag tggcttaaga atgctagttg
     2101 aaagcaaagt agattttgcg aacaatcgaa atttagttat agcttcagta attttagttg
     2161 taggtatcgg taatttagta tttaacttaa aagaaattgg tatcaacctt caaattgagg
     2221 ggatggcatt agctgcactt tcaggaatta ttttgaactt aatcttacct aaagagaaaa
     2281 aacaaaacaa ttaagattta caaattaagg agggcgcttt tatgaatcat ttattatcaa
     2341 tggaacattt atctacagat caaatataca aacttatcca aaaggcaagt caatttaaat
     2401 ctggtgaacg tcaactacca aactttgaag ggaaatatgt cgcaaattta ttctttgaaa
     2461 attctactcg aacaaaatgt agttttgaaa tggcagaact taagctaggg ttaaaaacga
     2521 ttagctttga aacatcaaca tcatctgttt caaaaggtga atctttatat gacacatgta
     2581 aaactttaga aagtattggc tgtgatttat tagtcattag acatccgttt aataactact
     2641 atgaaaaatt agcgaatatt aacatcccaa ttgcgaatgc tggtgatggt agtggacaac
     2701 atccaacaca aagtttactt gatttaatga cgatatatga agaatatgga tattttgaag
     2761 gcttgaatgt attgatttgt ggagacatta aaaattcacg tgtcgcacgt agtaattacc
     2821 atagtttaaa agcattaggt gcaaacgtaa tgtttaatag cccaaatgct tggattgatg
     2881 attctttaga agcaccttat gtaaatatag atgatgttat agaaacagta gatatagtta
     2941 tgttattaag aattcaacat gaaagacatg ggcttgcaga agaaactaga tttgcagcag
     3001 atgattatca tcaaaagcat ggcttaaatg aagtgcgcta taacaaatta caagaacatg
     3061 ctattgttat gcatccggca cctgtgaata gaggagtaga aatacaaagc gatttagtag
     3121 aagcttcaaa atcaagaatt tttaagcaaa tggaaaatgg cgtttactta agaatggcag
     3181 tcattgatga attattaaaa taggtaaggg gacgaaaatg atgaaattaa ttaaaaacgg
     3241 taaagtatta caaaatggcg aattacaaca agcagatatt ttaattgatg gtaaggtaat
     3301 taaacaaatt gcacctgcaa ttgaaccaag caatggtgtt gacatcatag atgcgaaagg
     3361 tcactttgtg tcacctggat ttgtcgatgt tcatgttcat ttacgtgaac ctggtggtga
     3421 atataaagag acaattgaaa ctggtactaa agctgctgct agaggcggat ttacaactgt
     3481 atgtccaatg cctaacacaa gaccggtacc agattctgta gaacattttg aagctttaca
     3541 aaaattaatc gatgacaatg ctcaagtacg tgtattacct tatgcttcaa ttacaacacg
     3601 tcaattaggt aaagaattgg ttgatttccc agcactagta aaagaaggtg cctttgcgtt
     3661 tacagatgac ggtgtaggag tacaaactgc aagcatgatg tatgaaggta tgattgaagc
     3721 tgcaaaagta aacaaagcca tcgtagcaca ctgtgaagat aattcattaa tctatggtgg
     3781 tgcaatgcat gaagggaaac gcagtaaaga gttaggtata ccaggtattc caaacatttg
     3841 tgaatctgtt caaatcgcaa gagatgtact attagctgaa gcagcaggtt gtcattatca
     3901 tgtatgtcat gtttctacta aagaaagtgt tagagtcatt cgtgatgcta aacgcgcagg
     3961 cattcatgtt acagctgaag ttacaccaca tcatttattg ttaacagaag atgatattcc
     4021 tggtaataat gccatttata aaatgaatcc accattgaga agtactgaag atagagaggc
     4081 tttgttagaa gggttactag acggtacaat tgattgtatc gcaacagacc atgcaccaca
     4141 tgcacgtgat gaaaaagcgc aaccaatgga aaaagcacca ttcggaattg ttggtagtga
     4201 aacagcattc ccattattat atacgcattt tgtaaaaaat ggtgattgga cattacaaca
     4261 attagtagat tacttaacaa ttaaaccatg tgagacattt aatttagaat acggcacatt
     4321 aaaagaaaat ggttatgcag atttaacaat cattgattta gatagtgaac aagaaattaa
     4381 aggagaagat ttcttatcaa aagcagataa tacaccattt atcggctata aagtttatgg
     4441 aaatccgatc ttaacaatgg ttgaaggcga agttaaattt gagggggata aataatatgc
     4501 aaagcaaacg ttatctagtg ttagaagacg gttcttttta cgaaggctac cgtttaggat
     4561 ctgataactt aactgtagga gaaattgtat ttaatacagc gatgacaggt tatcaagaaa
     4621 ctatttcaga tccatcatat acaggtcaga tcattacttt tacgtatcca ttaatcggta
     4681 attatggtat caatagagac gattttgaat cattagtacc tacattaaac ggtattgtag
     4741 tgaaagaagc gagtgcgcat ccaagtaatt ttagacaaca aaagacactt catgacgttt
     4801 tagaattgca tcaaattcca gggattgcag gtgttgatac aagaagtatt acgcgtaaaa
     4861 ttcgacaaca cggtgtgtta aaagctggtt ttactgatcg aaaagaagat attgatcaac
     4921 ttgtcaaaca tttacaacaa gtagaattac ctaaaaacga agtagaaatc gtttcgacta
     4981 aaacaccgta tgtttcgaca ggtaaggatc taagtgtcgt acttgtagac tttggtaaga
     5041 agcaaaatat tgttcgagaa ttaaacgtca gaggttgtaa cgtcacagtt gtaccatata
     5101 caactactgc cgaagaaatt ttagcaatgg ctccagatgg cgttatgcta tcaaacggac
     5161 caggtaatcc tgaagttgta gaatgtgcga ttccaatgat tcaaggaatt ttagggaaaa
     5221 ttccgttctt tggtatctgt ctaggacatc aactttttgc attatctcaa ggagcaagct
     5281 catttaaaat gaagtttggt catcgtggtg cgaaccatcc agttaaaaat ttagagactg
     5341 gaaaagttga tattacgagt caaaaccatg gatatgcaat agatatagat tcgttaaaaa
     5401 gtactgattt agaagttact catcttgcat taaatgatgg tactgtagaa ggtttaaaac
     5461 ataaaacatt accagcattt tctgttcaat accatcctga agcaaatcca ggaccgtcag
     5521 attcaaacta tctatttgat gattttgtag caatgatgac taattttaag gaaaaggagc
     5581 gtcatatcaa tgcctaaacg taatgatatc aaaacaattt tagtaatagg gtctgggcca
     5641 attatcatag gtcaagcagc tgaatttgat tatgctggaa cacaagcatg tctagcttta
     5701 aaagaagagg gatatcgagt tattcttgta aattcaaatc cagcgacaat catgactgat
     5761 aaggaaattg cggataaagt atatatcgaa ccgttaactc atgattttat agcgcgaatt
     5821 atacgtaaag agcaacctga cgctttactt ccaactttag gtggtcaaac aggtttaaac
     5881 atggcgattc aactacacga aagtggtgtg cttcaagata ataacgtcca attattagga
     5941 actgagctaa catcaattca acaagcagaa gaccgtgaaa tgtttagaac attaatgaat
     6001 gatttaaacg ttcctgtacc agagagtgac attgtaaata cagtagagca agcctttaaa
     6061 ttcaaagagc aagtgggata cccgctaatt gttagaccgg catttacgat gggtggtacc
     6121 ggaggcggta tttgtcataa tgatgaagaa ttacatgaaa tcgtctcaaa tggtcttcat
     6181 tatagtccag caacgcaatg tttattagaa aaatctatcg caggttttaa agaaatcgaa
     6241 tacgaagtaa tgcgtgataa aaacgataat gccatcgttg tatgtaacat ggaaaatatt
     6301 gatccagttg gtattcatac tggcgattca attgttgtgg ctcctagtca aacattatca
     6361 gatgttgagt atcaaatgtt acgtgatgtt tcattaaaag ttattcgagc tttaggtatc
     6421 gaaggtggtt gtaatgttca attagcatta gacccccatt cattcgatta ttatattata
     6481 gaagtaaatc cgcgtgtatc acgttcatca gcgttagctt caaaagcaac aggatatcct
     6541 attgcaaaat tagctgctaa aatcgcggtt ggtctaacat tagatgaaat gttaaatcca
     6601 attacaggaa catcttatgc agcgtttgaa ccaactttag actatgtgat ttcaaaaata
     6661 ccaagatttc cttttgataa atttgaaaaa ggagaacgag agcttggcac acaaatgaaa
     6721 gcaacaggtg aagttatggc cattggtcga acttacgaag aatcattgtt aaaagcaatt
     6781 cgatcacttg agtatggtgt gcatcactta ggattaccaa atggtgaaag cttcgatctt
     6841 gattatatta aagaacgtat ttcacaccaa gatgatgaac gattattttt catcggcgaa
     6901 gcaattagaa gaggcacaac attagaagaa attcataata tgactcagat tgattacttc
     6961 ttcttacaca agttccaaaa cattattgat attgagcatc aactaaaaga gcatcaaggt
     7021 gatttagaat atcttaaata tgcaaaagat tatggattta gtgataaaac aatagcgcat
     7081 cgctttaata tgacggaaga agaagtatat caattgcgta tggaaaatga tattaaacct
     7141 gtttacaaga tggttgatac ttgcgcagct gaatttgaat cttcaacacc atattattat
     7201 ggtacatacg aaactgaaaa tgaatccata gttactgaca aagaaaaaat cttagtatta
     7261 ggctctggac caattcgaat cggccaaggt gtagaatttg actatgcgac agttcacgcc
     7321 gtttgggcaa ttcaaaaagc agggtacgaa gcgataattg tgaataacaa tccagaaaca
     7381 gtttcaacag acttctcaat ttctgacaaa ttatactttg aacctttaac tgaagaagat
     7441 gtgatgaata tcattaattt agaaaaacct aaaggtgtcg ttgtacaatt tggaggacaa
     7501 acagcgatta atttagcaga caaattggct aaacatggtg ttaaaatact tggtacttca
     7561 ctagaaaatc taaatcgtgc tgaagataga aaagaatttg aagcactatt aagaaaaatt
     7621 aacgtgccac agccacaagg gaaaacagct acatcacctg aggaagcatt agcgaatgct
     7681 gcagaaatcg gatatccggt tgtagtaaga ccttcttatg tattaggtgg tcgcgcaatg
     7741 gaaattgtag acaatgacaa agagttagaa aactatatga cccaggctgt aaaagcgagt
     7801 ccggaacatc cggtactagt cgatagatat ttaactggta aagaaattga agttgatgcg
     7861 atttgtgatg gagaaacggt cattattcca ggaataatgg aacatattga acgtgctggt
     7921 gtgcatagtg gtgactcaat cgctgtatat ccaccacaaa ctttgacaga agacgagtta
     7981 gcaacacttg aggactatac tataaaatta gctaaaggtt taaacatcat tggcttaatc
     8041 aacattcaat tcgttatagc tcacgatggt gtgtatgttt tagaagtaaa tccacgttct
     8101 agtagaacgg taccattctt aagtaaaatt actgatattc caatggcaca attagctatg
     8161 cgagcaatca ttggggaaaa actaacagat atgggttatc aagaaggggt tcaaccatat
     8221 gctgagggtg tctttgtgaa agcaccggta tttagtttca ataaattgaa aaatgttgat
     8281 attactttag gacctgaaat gaagtcaaca ggtgaagtga tggggaaaga tactacatta
     8341 gaaaaggcgt tattcaaagg gttaacaggt agtggtgttg aagttaaaga tcacggtaca
     8401 gtattaatga ccgtcagtga caaagataaa gaggaagttg ttaaattggc acaacgctta
     8461 aatgaagttg gctataaaat tttagcaacg tctggaacag ctaataaatt agctgagtat
     8521 gacatacctg cagaagtagt aggcaaaatt ggtggcgaaa atgatttatt aacacgtatt
     8581 caaaatggtg atgttcaaat cgttataaat acaatgacta aaggtaaaga agtagaaaga
     8641 gatggcttcc aaattagacg tactacagtt gaaaatggta ttccatgttt gacatcttta
     8701 gatacagcta atgccttaac gaatgtaatt gaaagtatga catttacaat gcgtcaaatg
     8761 taaatcaatc aaactgtatc ggtggggctg taattaacca tttacttaaa gaagtttata
     8821 ttacagcctc attattttaa tgaatttctt aatataaagg gagacatata tgatgaaaga
     8881 tttaccaatt attgcattag attttgaatc aaaagaaaaa gtaaatcaat ttttagattt
     8941 atttgatgaa tcattattcg taaaagtagg tatggaactt ttttatcaag aaggtcctca
     9001 attaattaat gagataaaag aaagaggcca tgatgtattt ttagatttaa aattgcatga
     9061 tattcctaat acagtaggta aggcgatgga aggactagct aaattgaatg ttgatctggt
     9121 aaatgttcat gctgctggtg gcgtaaaaat gatgtctgag gccattaaag gattaagaaa
     9181 acataatcaa catacaaaaa ttattgcagt aacacagctt acgtcaacaa cagaagacat
     9241 gttacgacac gaacaaaata tacaaacatc gattgaagag gccgttttaa attatgccaa
     9301 gttagcaaat gcagctggtt tagatggcgt tgtttgttca cctcttgaaa gtcgtatgtt
     9361 gactgaaaag ttaggtacat catttttaaa agtaacacca ggtattagac ctaaaggtgc
     9421 atctcaagat gaccaacacc gtattacgac accggaagaa gcaagacagc ttggttcgac
     9481 gcatattgta gtcggtagac cgattacaca aagtgacaat ccagtcgaaa gttatcataa
     9541 aattaaagaa agttggttag tataatggct aaagaaattg caaaatcatt attagatatt
     9601 gaagctgtaa cattatcacc aaatgattta tatacatgga gttcaggtat taaatcaccg
     9661 atttactgtg ataaccgtgt tacgttaggt tatcctttag ttcgaggcgc aatccgcgat
     9721 ggtttaatta acttaattaa agaacacttt cctgaagtag aagttatttc tggtactgca
     9781 acagctggta ttccacatgc agcttttatt gctgaaaaat taaaattacc aatgaattat
     9841 gttcgttcat caaataagag tcatggtaag caaaatcaaa tcgaaggtgc taaaagtgaa
     9901 ggtaaaaaag tagttgtgat agaagattta atttcgacag ggggatcttc agtcacagca
     9961 gttgaagcct taaaactagc aggtgcagaa gtattaggtg ttgtagctat ctttacttac
    10021 ggtttgaaaa aagcagatga tacatttagc aatattcaac taccttttta cactttaagt
    10081 gattacagtg aattaattga agtagctgaa aatgaaggta aaatttctag tgaagatatc
    10141 caaacattag ttgaatggag agacaactta gcataatata gacactagaa ggaggaattc
    10201 aacaaatgaa tgacaaaaca tctaatgatt tatatgggaa gataaaacat tgtaacgaat
    10261 ttatcaatca ttcaaatgat tccaatctat ctagtagtca cgatgtcgac gaaagttcaa
    10321 cgaagcaaaa acatataaaa aataaaacaa ctatagacca taatgatgat ttatttaaac
    10381 atgtaaagga tatattacgt aaacaaggac aaatttaaaa acaaaaaagc tatagtgttt
    10441 aatatcaggg gctgcttaac cgcatccgaa tattacaaac actatagctt ttattatata
    10501 aaaaatttat tagcggataa ttaactttat taaaatccaa ccaattaaga atacgagtaa
    10561 taggactaat actggaatca cataatgtaa cataacgtcc ctcctttaac ttaattttaa
    10621 ttgtaatcaa atttgacaat aagtcaaaac attaatacct atgataagta tcatttatta
    10681 acatatgtat catattttta atcttgcgta atttttatcg ttaactatgt gatttaatca
    10741 acaggccaat aagacgtttg tcattcgtgc acataacggt acgacgcgct tttgtcattt
    10801 taattatgtt aagataatag tagttataga agttcaatct ataggaggca tagcatggat
    10861 attccaaaaa tcacgacatt tttaatgttt aataaccaag ctgaagaagc tgttaaacta
    10921 tacacaagct tatttgaaga tagtgagatt ataacaatgg ctaagtatgg tgaaaatgga
    10981 cctggtgatc ccgggactgt acaacactca atatttacat taaatggaca agtattcatg
    11041 gcgattgatg ctaatagtgg cacagaatta ccaatgaatc ctgcgatttc attatttgtt
    11101 acagtaaaag atactattga aatggaacga ctatttaatg gattaaaaga tgaaggtgcc
    11161 attttaatgc caaaaacgaa tatgccacca tacagagagt ttgcttgggt tcaagataag
    11221 tttggagtaa gttttcaatt agcattacct gagtaaaagg attcgcacag cgttgaatga
    11281 taaagaaaca ctttttctta tgcatgcgtt ttacctatgt atttctattt taagtacaca
    11341 ttagcatttt agtttcactc atttttaaaa tcacaaagat aatttgtaac ttaaatgatg
    11401 atacgttatt taaaaacacg atacttcgtt tcaatgaacg cattaaataa taaataaaca
    11461 cctcaccata agaaaggact actttctttt gtgaggtgtt tatttgtcga caacttgatt
    11521 atgattgttt cattttttga ataagttcat aatcaggtgt agcatacaaa gttttttgat
    11581 tgtcatatgt tacaaaccca ggctttgcac ctgatggttt gtgcacattt ttaattaatg
    11641 tgtaatcaac aggtatttgt ccagaattac cagcttttga aaagtatcct gctaacatag
    11701 ccgcttcctt gattgtcgta tcacttggtg catcattaaa tataacgaca tgtgaaccag
    11761 gaatatcttt tgtgtgtaac catgtgtgag tttttttagc ttttttattt gttaaataat
    11821 cattttgctt gttattcttg ccaacatata tatcgtcgcc atcagttgat acataatgtt
    11881 gtaattgaat ctgcgctttc tttttcttag tttgattttt acgctgtttc ataaagcctt
    11941 gttctgctaa ttcatctcta atttcatcaa tgtcatggac agaaatatga tgtaattgtt
    12001 gttcgattgt tgaaaaataa tctatattgt ctttcgtcaa ttgaatttga tgttgtaatt
    12061 cacgttctct cgttttcata cgattatatt gtttataata atattgagca tttgctgatg
    12121 gggattttgt aggatttaaa ggaatgacaa cttcttcatt cgtataataa ttcaatgccg
    12181 tcacttcttt atcgccttgc ttaattcgat atatattagc agtgatcaat tcaccatata
    12241 actgttcagt atctttattt ttagactgct catattcttc aatcaacttc gctaatttat
    12301 tttgatattt gtgcaactgc tgttgaacaa atcgaactaa atcattcgca cgttgtttaa
    12361 cgcgttcacg ttcgccacgc gcatcataaa aacgatcaag taaatcattt aatgaatcgt
    12421 atgtaactgt atcatcatta aattgattta actttataaa atagaaatcc tctttacctg
    12481 tttcatgatt tttatgaaaa ataggagtag gtggtaactt ggtttctgcc attacttcgt
    12541 caaatgcttc tggtaatgtt gatgaagtca taaattgacg acgactaaca atttcattcg
    12601 taattaaagg gctaaatcct tcaaactgat tcaataattg tttagcaata ttacctgcgt
    12661 taaaatcgat atatttcaac acctctgcac ctgtaatatc atacggattt attttgtgct
    12721 gagtaggtgg tgcttcataa ttaaatcctg gcattactgt acgatagtga ttcgtatttg
    12781 gtgttaagtg tttaaatcct tcaattattt tgcgattttc atctactaaa attaagttac
    12841 tatgtttacc cataatctca aggatgacag tgcggtaaat agtatcgcca atttcatctt
    12901 tactctttat ttcgatttca atgcgacgat cattaccaat ttgcttaatc gatttgataa
    12961 taccaccttc taagtgtttt ctaaaaacac gcgcaaacat gggtggatta aatggattat
    13021 catatttttt agtagttaat tgtaatcttg aaaagtttgg atggattgac aataacaatt
    13081 gatggttttg tctattttga cgtacaacca ttagtatcgt gtcattatca ggttgattga
    13141 ttttgtgaac gcgtcctgtt gttaaaaatt gtagagactc aaccattttc tttgtaaata
    13201 agccatcata agccataatt tatcttccac cttcttcata tcattcgttt cattttaaca
    13261 tgattgtaac ttttcggtat agttattgaa ccatacatta tgccttacat tttacattat
    13321 ctaaaagttt gcaattcaat ttatttaatt aaaattaatc gtgcaatata acgatattgt
    13381 tagacttaat agaaattatg gcatgcaatt tcaaatatgc tatacaatat aaagaacaat
    13441 gtgatatcat atttaaataa tagaagatta gcttagagag gtcgtaaggc atggataatg
    13501 aaaaaggatt gttaatcgtt ttatcaggac catctggagt aggtaaaggt actgttagaa
    13561 aacgaatatt tgaagatcca agtacatcat ataagtattc tatttcaatg acaacacgtc
    13621 aaatgcgtga aggtgaagtt gatggcgtag attacttttt taaaactagg gatgcgtttg
    13681 aagctttaat caaagatgac caatttatag aatatgctga atatgtaggc aactattatg
    13741 gtacaccagt tcaatatgtt aaagatacaa tggacgaagg tcatgatgta tttttagaaa
    13801 ttgaagtaga aggtgcaaag caagttagaa agaaatttcc agatgcgcta tttattttct
    13861 tagcacctcc aagtttagaa cacttgagag agcgattagt aggtagagga acagaatctg
    13921 atgagaaaat acaaagtcgt attaacgaag cgcgtaaaga agttgaaatg atgaatttat
    13981 acgattacgt tgtagttaat gatgaagtag aacttgcgaa gaatagaatt caatgtattg
    14041 tagaagctga gcacttaaaa agagagcgcg tagaagctaa gtatagaaaa atgattttgg
    14101 aggctaaaaa ataatgttaa atccaccttt aaaccaatta acgtcacaaa ttaaatcaaa
    14161 gtatttaatt gcaacaactg cagcgaaaag agcgcgtgaa attgatgaac aacctgaaac
    14221 tgaattatta agtgaatatc attcatttaa accagttggt agagcgttag aagaaattgc
    14281 tgacggtaca attcgccctg ttatttcaag tgattattat ggtaaagaat agtttcgacc
    14341 attaaaaata tgtgaagcta gacgtacatc aaatacgtca aggcatatcc aaatcaaacg
    14401 cacctatgta ggtgtgtttt tttagtttat ccaatgattg atgttataat aatactaaat
    14461 ttgtatctat aaaaaagtaa tgagcatttg tgcgcatatg atgatgtaaa gcgtaaatgt
    14521 catagtgata tttaagggag attgaatatg aagaaaatat tattagccgt tacaggtggc
    14581 attgcggcat ataaagcaat tgatttgaca agtaagttaa cacaatctgg gtatgaagtt
    14641 cgcgttatgt taacgaatca cgcacaaaaa tttgtgacac cattagcatt tcaagcaata
    14701 agtcgaaatg ctgtttatac agatactttt atagaagaaa atccttcaga aatacagcat
    14761 attgcattag gtgattgggc agatgcaatc attgttgcac ctgcaacggc aaatacaatt
    14821 gcaaaattga gtgtaggtat tgctgatgat ttggtgacat caacgttgct agcaacagag
    14881 acaccgaaat ttattgcgcc tgctatgaat gtgcatatgt atgaaaataa acgtacgcag
    14941 caaaatatta atattttaaa agaagatggg tatcatttta tcgaaccagg aagcggattt
    15001 ctagcatgtg gttatgttgc taaaggacgt atggaagaac cgcttcaaat cgtttctgta
    15061 attgatgctc attttcaaaa tagtaatcgt ttagctaata gttcatttca agataaacgc
    15121 gcattggtta cagcaggacc aactattgaa gttatcgatc cagtcagatt tgtatccaat
    15181 cgttcttctg gaaaaatggg ttatgcaata gctgaagcat tgcgaaatcg aggagctatc
    15241 gtgacgttag ttgctggtcc tacaacacta gaggatccaa aagatattga agttattcat
    15301 gttcaaagtg ctgaagaaat gtttgaacaa gtgacaagcc gatttgacga acaagatatt
    15361 gttgtaaaag cagcagccgt atctgactat acaccggttg atgtattaga acataagatg
    15421 aaaaagcagg atggtgattt gtcagtatct tttaaacgta ctaaagacat tcttaaatat
    15481 ttaggtgaac ataaaacatc acagtattta ataggctttg cagcagagac tgaagatatt
    15541 gaaaattatg cacaacaaaa attacgcaag aaaaatgcag atgtgattat ttcaaataat
    15601 gttggggata tgtctatcgg atttagttct gatgataatg aattgacaat gcattttaaa
    15661 aataatgaaa aggtaaatat caagaaagga aaaaaagtag tattagctgc acaaatttta
    15721 gatgaactag aaactaggtg gcaataatga tagcgaaagt catagtcgat gtcgcgtcga
    15781 agagcgttga ctataaattt gattatataa ttcccgaaca actcgaatct gtcatccaac
    15841 ctggtgtgcg tgtaattgta ccttttggac caagaacgat tcaaggttat gtaatggaag
    15901 taacagcaga acctgatgca caacttgacg tttcgaagtt aaaaaaaatc atagaagtga
    15961 aagatataca accagaatta acatcagaat taatagcttt aagtgagtgg atgggttcaa
    16021 ctcatgtcat taaacgtatt tctatgctag aagtgatgct tccgagtgct attaaagcga
    16081 agtataaaaa agcatttaag atgaaagatg acatagagct accttcagct ttattacaaa
    16141 aatttgataa gcatggttac tattattata aagatgcgca aaaaaataat gatattcaat
    16201 tgcttatgaa gttgttaaaa gatgatatcg ttgaagaaaa aacgattctc acacaaaata
    16261 taactaaaaa aaccaagcgt gctgttcgtg tcattgaagg gtatcatcct gatgaagtat
    16321 tagctaagtt ggagaaagtt attaaacaat acgatttgta tgcttacttg tctgaagaac
    16381 aacataaaac aatattttta actgatattg aggatatggg cttttcaaaa tccagtttag
    16441 atggacttat caaaaaaggt tatgttgaaa aatatgacgc ggttgttgaa agagacccat
    16501 ttaaagatcg tgttttcgaa caagaatcaa aacagcaatt aacagaagac caatataaag
    16561 catatgaagc gattaaagct aaaattgtaa gccaagagca agaaacattt ttacttcatg
    16621 gtgtgacggg atcaggtaaa acagaagtat atttacaaac gatagaagat gttttaagcc
    16681 aaggaaaaca ggcgatgatg ttagttcctg aaatcgctct aacaccgcaa atggttttac
    16741 gcttcaaacg tcgatttggt gatgacgttg ctgtattaca ttctggctta tctaatgggg
    16801 aacgttatga tgagtggcaa aaaattaggg atggtcgtgc gagagtaagt gttggtgcaa
    16861 ggtcaagtgt gttcgcacct ttcaaaaatt tagggttaat catcattgat gaagaacatg
    16921 aatctacata taaacaagaa gattatccga gatatcacgc tagagaaatt gcccaatggc
    16981 gaagtgaata tcatcactgt ccagtcattt taggaagtgc aacaccatgt cttgaaagtt
    17041 atgcacgagc tgaaaaaggc gtttatcatt tgctatcatt accaaacaga gtgaaccaac
    17101 aagctttacc tgaaattgat atagtagaca tgcgtgaaga attgagtgaa ggtaatcggt
    17161 caatgttttc aaaagattta cgtgaagcca tacaattaag attagatcga caggaacaag
    17221 ttgttttatt tttaaatcga cgtggttatg catcgtttat gttatgtcgg gattgtggat
    17281 atgtaccgca atgtccaaac tgtgatattt cattaacgta tcataaaacg acagacttat
    17341 taaaatgtca ctattgtggt taccaagaga cgccaccgaa tcaatgtcca aattgtgaga
    17401 gtgaacacat tcgacaagta ggcactggta ctcagaaagt tgaagaacta ttgcaacaag
    17461 aatttgaaga tgcgcgcata attaggatgg atgtagatac aacctcaaag aaaggtgcac
    17521 atgaaaagtt attgactgaa ttcgaaaaag gtaacggtga cattttacta ggtactcaga
    17581 tgattgcgaa aggattagat tatccaaata ttactttagt tggtgtgctg aatgcagata
    17641 caatgttaaa tttacctgat tttcgggcga gcgaacgtac ttatcaacta ttaacgcaag
    17701 tggctggtag agctggtcgt catgaaaagg caggtcaagt catcattcaa acgtataatc
    17761 cagatcatta ttcaatattg gatgttcaaa aaaatgatta tttaacattt tatcgtcagg
    17821 aaatggaata tcgtaaatta ggaaagtatc caccgtatta ttatttgatt aatttcacaa
    17881 tctcacataa agaaatgaag aaggttatgg aagcatcgca gcatgttcat aaaattttat
    17941 tacagcattt aacagaaaaa gcgcttgtac taggtccatc tccggcagca cttgcgagaa
    18001 tcaacaatga atttagattc caaattttag tgaaatataa aagtgaacct ggattattac
    18061 aagccattca gtttttagat gactattacc atgaaaaatt tataaaagaa aaattagcat
    18121 tgaagattga tattgatcca cagatgatga tgtaattttt tgactaaaac actttaatat
    18181 caatgtttcc taagagtatg aaaggtcttg tttttacttt aagtacagaa aaagggcaga
    18241 atatacaact gttaaccatt taaattagca gtttatattc tgtcttttta tatggcttta
    18301 taacttacgt gattttgatt tgattaggaa tttattagta ttttcattta cgaattctga
    18361 ttttaatgta ggtgccatac ttactgatga ataaaagtac taatgattaa acaaactatt
    18421 tttaaatggc aaattgaaat agtgcaaa
//
LOCUS       DAMZLE010000040        18266 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMZLE010000040 DAMZLE010000000
VERSION     DAMZLE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.040"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            57..2579
                     /gene="mprF"
                     /locus_tag="POB92_001223"
     CDS             57..2579
                     /gene="mprF"
                     /locus_tag="POB92_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDI6578732.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2782..3291)
                     /gene="msrA"
                     /locus_tag="POB92_001224"
     CDS             complement(2782..3291)
                     /gene="msrA"
                     /locus_tag="POB92_001224"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6578733.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3428..4411
                     /locus_tag="POB92_001225"
     CDS             3428..4411
                     /locus_tag="POB92_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI6578734.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4499..4684)
                     /locus_tag="POB92_001226"
     CDS             complement(4499..4684)
                     /locus_tag="POB92_001226"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDI6578735.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            4832..6094
                     /locus_tag="POB92_001227"
     CDS             4832..6094
                     /locus_tag="POB92_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDI6578736.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6230..7321)
                     /locus_tag="POB92_001228"
     CDS             complement(6230..7321)
                     /locus_tag="POB92_001228"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDI6578737.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7486..8517
                     /locus_tag="POB92_001229"
     CDS             7486..8517
                     /locus_tag="POB92_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6578738.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAIILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9009..10415
                     /locus_tag="POB92_001230"
     CDS             9009..10415
                     /locus_tag="POB92_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDI6578739.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQISERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            10412..10978
                     /locus_tag="POB92_001231"
     CDS             10412..10978
                     /locus_tag="POB92_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDI6578740.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            10984..11981
                     /gene="trpD"
                     /locus_tag="POB92_001232"
                     /pseudo
     CDS             10984..11981
                     /gene="trpD"
                     /locus_tag="POB92_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
     gene            11983..12765
                     /gene="trpC"
                     /locus_tag="POB92_001233"
     CDS             11983..12765
                     /gene="trpC"
                     /locus_tag="POB92_001233"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDI6578741.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            12765..13397
                     /locus_tag="POB92_001234"
     CDS             12765..13397
                     /locus_tag="POB92_001234"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDI6578742.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13390..14604
                     /gene="trpB"
                     /locus_tag="POB92_001235"
     CDS             13390..14604
                     /gene="trpB"
                     /locus_tag="POB92_001235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDI6578743.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGSAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            14597..15325
                     /locus_tag="POB92_001236"
     CDS             14597..15325
                     /locus_tag="POB92_001236"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI6578744.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            15647..16909
                     /gene="femA"
                     /locus_tag="POB92_001237"
     CDS             15647..16909
                     /gene="femA"
                     /locus_tag="POB92_001237"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDI6578745.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            16928..18187
                     /gene="femB"
                     /locus_tag="POB92_001238"
     CDS             16928..18187
                     /gene="femB"
                     /locus_tag="POB92_001238"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDI6578746.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT         6457 a         2610 c         3343 g         5856 t
ORIGIN      
        1 ggtttatatg atatattttg atttataaca gaaataatta gaattgatgt gaaaaaatga
       61 atcaggaagt taaaaacaaa atattttcaa tcttaaaaat tacgtttgct acagctttat
      121 ttatttttgt agtaatcaca ttgtatcggg agttatctgg tattaacttt aaagatacgt
      181 tggttgaatt tagtaagatt aaccgtatgt ccttagtttt actatttatt ggtggtgggg
      241 catcgcttgt tattctatca atgtatgatg tgattttatc tagagcttta aaaatggata
      301 tatccttagg caaagtttta agagtaagtt atatcatcaa tgcattgaat gcgattgtag
      361 gtttcggtgg ctttattggt gcaggcgtta gagctatggt ttataaaaac tatacgcatg
      421 ataaaaagaa attagttcac tttatatcct taatacttat ttcaatgttg acaggtttaa
      481 gcttattatc attgttaatt gtattccatg ttttcgatgc atctttaatt ttagataaga
      541 ttacatgggt aagatgggta ttatatgtag tgtcattttt cttaccatta ttcattattt
      601 attcaatggt tagaccacct gataaaaaca atcgttttgt aggattgtac tgcactttag
      661 tgtcgtgtgt tgaatggtta gcagctgcag ttgtattata tttctgtggt gtaattgttg
      721 acgctcatgt atcattcatg tcctttattg caatatttat cattgctgca ttatcaggtt
      781 tagtcagctt tattcctggt ggtttcggcg ctttcgattt agttgtatta ctaggattta
      841 aaactttagg tgtccctgag gaaaaagtat tattaatgct acttctatat cgttttgcgt
      901 actattttgt accggtaatt attgcattaa ttttatcatc atttgaattt ggtacatcag
      961 ctaagaagta cattgaggga tctaaatact ttattcctgc taaagatgtt acgtcatttt
     1021 taatgtctta tcaaaaggat attattgcta aaattccatc attatcatta gcaattttag
     1081 tattctttac aagtatgatc ttttttgtaa ataacttaac gattgtttac gatgctttat
     1141 atgatggaaa tcacttaacg tattatattc tattggcaat tcatactagt gcttgtttat
     1201 tacttttact gaatgtagtt ggtatttata agcaaagtag acgtgccatc atctttgcta
     1261 tgatttcaat tttattaatc acagtggcga cattcttcac ttacgcttca tatattttaa
     1321 taacatggtt agctattatt tttgttctgc ttattgtagc tttccgtaga gcacgtaggt
     1381 tgaaacgccc agtaagaatg agaaatatag tagcaatgct tttattcagt ttatttattt
     1441 tatatgttaa ccatatattt attgctggaa cgttatatgc attagatatt tatacgattg
     1501 aaatgcatac atctgtattg cgctattact tctggcttac gattttaatc atcgctatca
     1561 tcataggtat gattgcatgg ttgtttgatt atcaatttag caaagtacgc atttcttcta
     1621 aaattgaaga ttgcgaggag attattaatc agtatggcgg taattatttg agtcacttga
     1681 tatatagtgg tgacaagcag tttttcacta atgaaaataa aacagcattt ttaatgtatc
     1741 gttataaagc aagttcatta gtggttcttg gagatccgtt aggtgatgaa aatgcctttg
     1801 atgaattgtt agaagcattc tataattacg ctgagtattt aggctatgat gttatattct
     1861 atcaagttac agatcaacac atgcctttat atcataattt cggtaaccaa tttttcaaat
     1921 taggtgaaga agcaattatt gatttaacgc aattttcaac ttcaggtaaa aaacgccgtg
     1981 gatttagagc gactttaaac aaattcgatg aacttaatat ttcattcgaa attattgaac
     2041 caccgttttc aactgaattt ataaatgaac ttcaacatgt aagtgattta tggctagata
     2101 atcgtcagga aatgcatttc tctgtgggtc aatttaatga agaatactta tctaaagcgc
     2161 caattggtgt aatgcgaaat gaagaaaatg aagtaattgc attttgtagt ttaatgccaa
     2221 catactttaa tgatgccatt tcagtcgatt taattagatg gttgccagag ttagatttac
     2281 cattaatgga tggtctatac ttgcatatgt tactttggag taaagaacaa ggttatacaa
     2341 aatttaatat gggtatggca acgttatcga acgttggtca attgcattat tcatatttaa
     2401 gagaacgact tgcaggccgt gtctttgaac atttcaacgg tctatatcgt ttccaaggat
     2461 tacgtcgtta taaatctaaa tataatccga attgggaacc acgcttttta gtttatcgta
     2521 aagataattc gctttgggaa tcactttcta aagtaatgcg tgtaatacgt cacaaataat
     2581 taaaatccaa gtgctaagag gtatacagtt atgcctgaag cgcttggatt ttttggtttc
     2641 tataaagaat gatttataaa aaacggtaca gataagaata tttaagtttc ctatctgtac
     2701 cgtattttaa tattgaaatg agcttaagtt aagtcgccta acttttatta tcagtccaat
     2761 cagtttcatc aagtataaat gttattgctt atttttgtat tcttggcgta ttttttgttc
     2821 ttctgcatag cgctctggat ttttcttata aaaatcttgg tgatagtctt cggctttgta
     2881 aaattgtgac gcgggtaata tttttgttgc aattgcctta tcagcattaa tcgtattttt
     2941 aagctgctcg atataagtct cagcgagttc tttttgatga tcattagtgt agaaaatagc
     3001 tgtttgatat tgaggaccac ggtcttgata ttgaccccct gtatctaatg ggtcaatgac
     3061 tgagaaaaat atttctaata acttattgta tgagaataat gcaacatcat attgaatttc
     3121 aacagtttct aaatgaccac tcgtacctga ttttacttgt tcgtaagtag gattttcaat
     3181 atgtccgccc atatatccag aagttacttt ttctatgccg tcaaaggtgt caaatggttt
     3241 cgtcatacac caaaagcaac ctccggcaaa ataagctgta ttaatattca tttttgacat
     3301 cctttcatta gaccttagta cgatttatta agaaatcact tgctttttga attgttttta
     3361 tataacgtta atatgtgatt attatagtac acaaaaatct atttttgaag atataaggta
     3421 ggtaaatatg gataaagaaa ctaatgacaa cgaatataga cgtcaaagtg aacatcgcac
     3481 ttcggcgcct aagcgaaaaa agaagaagaa aattaggaaa ttacctatca ttcttctgat
     3541 tgttgtaatt ttacttatcg cattagttgt atatattgtg catagttaca atagcggtgt
     3601 agaatatgcc aagaaacatg cgaaagatgt taaagtacat caatttaatg gaccagtaaa
     3661 aaatgatggt aaaatttcta ttcttgtact cggtgcagat aaagcacaag gtggacaatc
     3721 aagaacagat tctatcatgg ttgttcaata tgactttatc aataaaaaga tgaaaatgat
     3781 gtctgtcatg cgtgatattt atgcagatat tccaggatat ggaaaacaca aaattaattc
     3841 agcatacgct ttaggtggtc cagagctact tagaaaaaca cttgataaaa atttaggaat
     3901 taatcctgaa tattatgcag tagttgattt tactggattt gagaaaatga ttgatgaatt
     3961 aatgccagaa ggtgtaccaa ttaatgtcga aaaggatatg tcgaaaaata ttggtgtatc
     4021 tttgaaaaag ggtaaccata ggttgaatgg taaagaatta cttggttatg caagattccg
     4081 tcacgaccct gaaggtgact tcggacgcgt gcgacgtcag caacaagtga tgcaaacatt
     4141 gaaaaaagaa atggttaatt ttagaacagt tgttaaatta ccaaaagttg caggtatttt
     4201 aagaggctat gtgaatacaa acattcctga ttcagggatt ttccaaacag gtttgagttt
     4261 tggtatccga ggtgaaaaag atgttaagtc attgactgtg ccaatcaaga actcatacga
     4321 agatgtcaat acaaatactg atggtagtgc attacagatt aataaaaaca caaataaaca
     4381 agctattaaa gactttttag atgaagatta aaaataaaca aggcgatttc tatcatactc
     4441 agatagaagt cgccttgttc gttttcaaag aattaattgg catgcatctt atgaagaatt
     4501 attgatctga ctttcttacg ccagccacac cataatggtt tggtttcatt tcttctataa
     4561 caacgtgaat tgcttgtcta tttgcccccg ttgttttttc tacggcgtca gttacttcgc
     4621 taactaaatt ttttaattgt tcatccgaac gaccttctaa taattttaca ttgacgattg
     4681 gcatcatcca aacctcctaa tcattattaa tttgagtata tcatgtaata cttaaaaatt
     4741 catttgcaaa ataacagaac gtttgttcta aaatagttga aatggaacac gtgttcgtat
     4801 ataatgagat tcaagtaagg gaggaataac agtgtataat tatcatttat tagaagatag
     4861 ggatgttcta tgtattgacc aaaaaagttt ttttgcgagt gtttcttgta ttgaaaaggg
     4921 gctagatcca ttagaaacaa agctagctgt tgttgcagat actaagcgtc agggttctgt
     4981 aatattggct gcgacaccta aattaaaaga attaggcatc aagacagggt cgcgattgtt
     5041 tgaaatacca catagaaatg atatttacat tatcaatcca agtatgcgta aatatcttaa
     5101 tgtttcagtt gctatttcta agattgcatt gcgttatatt ccacctgaag atttacacca
     5161 atatagtatt gacgaatttt ttatggatgt tactgatagc tatcatagat ttagttctac
     5221 agtacatgca ttttgcgaaa gacttaaacg tgaaatttat gaagaaacag gcatttattg
     5281 tactgtgggc attggttcta atatgttatt aagtaaaatt gctatggatg ttgaagcgaa
     5341 gcatagtcaa aatggtatag ctgaatggcg atatcaagat gtaccaacga aattatggcc
     5401 aattcagccc ttgcgagatt tttggggtat taatcgtcga acagaagcca aattgaataa
     5461 aagaggaatt tttactatag gagatttagc gaaatatcca tataaatttt taaaaaaaga
     5521 gttcggtatt ttaggtgttg atatgcatct acatgcgaat gggatagatc agagtaaagt
     5581 acgtgaaaaa cacaagatca gcaatccatc gatatgcaaa agtcaaatat taatgagaga
     5641 ttatcatttt gatgaagcaa aagtagtaat gcaagagtta attgaagatg ttgctagcag
     5701 agttcgagca agaaaaaaag tggcaagaac gatacatttt gcctttggct atagtgatga
     5761 aggcggtgta cataagcaat atactttgaa agatccaaca aacttagaaa aagatattta
     5821 taaagtagta atgcatttcg cagataaatt atgtaataaa caagcactat atcgtacgct
     5881 aagtatatct ttgagtcaat ttattaatga ggatgagcga cagttaagtc tgtttgaaga
     5941 tgaataccaa cgcaaacgtg acgaatgtct agctaaaacg atagaccaat tacatttgaa
     6001 atacggcaaa ggtattgtgt ccaaagcagt atcgtttaca gaagcaggta caaaacacgg
     6061 cagattaggt ttaatggctg gacataaaat gtaatgacta tacggtttaa gtaatatata
     6121 actgtgattc gtataaaata agtctctaaa gataaatatt tcatatatca caatagattt
     6181 cacaataata tctaagaata catggaattt atcaaaagag acttaataat tattggatat
     6241 aacaatcaaa atcactcaat gcttgcatac cgcgttctcg gtcagtaggg tttttgaaac
     6301 taatttttaa agcaccgtat atatcttcgc gtacttctaa gattcttaag ttgcttatag
     6361 atatgttatg taaactcagg atataagtca ctttacttat catacctgat tcatccggaa
     6421 tgtctacata tagatcatac gcagtattta gtccacctag ttgtttagcg ggtagtgcgt
     6481 cgcgatacga tttagcttgg gcaaaaaatg ataacaattt ttcagaatca ttgctttcaa
     6541 ttagtctttc taaatcttga aactgacttt ttagctgtcg aatcatttct aaaatatacg
     6601 ttttattact caaggtgata tctttccaca tttgtgcatt actactagct atacgagtga
     6661 tatcacgaaa accaccagct gcaagtttat taactaaatg atgttcttga ccgttctttt
     6721 gactaacatg aactaaacta gatgcaacga tatgaggtaa atgacttacg acgcttgtta
     6781 cgtagtcgtg ttcttcagca gtagttacaa taaatttagc aagagtaggt gataacagtt
     6841 cttttaacgt gtttgctgct tgctcatttc ttggctcatt gtagactaaa atataataag
     6901 cgttttcaaa taagtgcttt ttagcattta gtacaccaga tttatgacta ccagccattg
     6961 gatgaccact gactaaatga atattatgct ttaataaatt gcattcgtgt tgctgtatca
     7021 ttgctttagt actaccagta tcagaaacaa taacaccagg tttagttggc atatctataa
     7081 gctcgctaag atatttattt gtgatagcaa caggtgttgc ataaataatt acatcggctt
     7141 ttttaatagc ttcactataa tttaaacatt tttcattaat aatgccgatt gatttagctt
     7201 tatctaactg agaagtatct gcatcgtatg caataatatt agtattaggg ttatggtatt
     7261 ttatattgct agcaagactt ccaccaatta atccaagccc aacaaataaa actgttgtca
     7321 tataaatcac cttatttcga aattttcaga ataataacat tgtaaatgag ctgttgacac
     7381 agtgcaatag taaataaaaa tcgataatag cattaataga taaacggaga taaatcatct
     7441 acaataaaga gtatagtaac acaatggcaa cggaggggta aatcaatgga accaatatta
     7501 gaaatgatta aaacattaac aggtattaat agtccttcag gagacacaga agaagcaatt
     7561 caatttgtcg aaaaatatgc aaaagacttg ggttatcaaa caacactaac aaataaaggt
     7621 gcgttattaa taacagtgcc aggcaaaaat gatgaagtac aacgctgtat tactgctcat
     7681 gttgatactt taggtgcaat ggttaaagaa attaaagaag atggtcgctt agcaatagaa
     7741 ttaattggag gattcacgta taacgcgatt gagggtgaat attgccaaat taaaactgat
     7801 gctggtcaaa tatatacagg aacaatttgt ctgcatgaaa caagtgttca tgtatataga
     7861 aataatcatg aaatacctag agatcaaaag catatggaaa taagaattga tgaagtaact
     7921 acatcagaag aagatacaaa gagtttaggt atttcagtag gtgattttgt tagctttgat
     7981 ccacgtacag ttatcacgtc atcaggtttt attaaatctc gtcatttaga tgataaagct
     8041 agcgtagcga ttatactaca attactaaag aaattaaaag aagagcaaat aatattacca
     8101 catacaacgc aattttatat ttctaataac gaagaaatag gttacggtgc aaatgcatca
     8161 attgattcga aaatcaaaga atatattgca ttagatatgg gcgcgttggg agacggtcaa
     8221 gcatcggatg aatatacagt ttctatttgt gccaaagatg cttcaggtcc atatcataag
     8281 caattgaaat cgcacctagt taatctttgc aaaataaata acattccata taaagtcgac
     8341 atatatccat attatggttc agatgcttca gcagctttac atgctggtgc ggatatcaga
     8401 catggtttat ttggcgctgg cattgaatca tctcatgcaa tggaacgaac acatattgat
     8461 tctattaaag cgacagagaa attactatat gcatattgct tatcaccaat tgagtaaaca
     8521 attagtgttg acaaatgtga acgacctatg taatataatg aactataaaa ataattagaa
     8581 ttttctaaag aaatagtagc agatatgaaa cgtagcaaat agaaagctaa tgggtgatgg
     8641 gaattagcac gccatatctt gtgaattgga ctttggaaaa caattgaatg agttttgaaa
     8701 gtgaacatga attatgttaa ctaaggtggc accacggtaa cgcgtcctta caggtatatg
     8761 cgttatgtgg tgtcttttta tttagacaaa atgtagtagt taattaaagg tagcaacaga
     8821 aagttagtgg atgatgtgaa ctaacaccga gattaatgaa attgggtttt gtctgcaaca
     8881 gaaaaattat atatagtaaa gagtgaacta tgaatatttc gaatattcgg ttaatttagg
     8941 tggtaccacg cgtcagcgtc ctttatattg ataaggatgc tggcgctttt ttgaaaggag
     9001 cgtatagaat ggatatattt tataaaaaaa taaaagcaaa tgtaacgcca gaagttttag
     9061 cacaacttca ttccaagaag atcattttgg aaagtacaaa tcaacaacaa actaaaggtc
     9121 gctattcagt tgttattttt gatatttatg gcactttaac tttagataat gatgtattat
     9181 cagtaagcac tttaaaagaa tcgtatcaaa tcagtgaaag accgtaccat tatttaacga
     9241 ctaaaataaa tgaagactac cataatattc aagatgagca acttaagtca ttaccgttta
     9301 tatctggata tgttgggacg tgtagctttg atttagtaag acatgaattt cctaaattgc
     9361 aatcaataca attagaagat cacaagcagc acgatgtaag gttatatatg gttgaacaag
     9421 tttatgtatt tgaccattac aaagatgagt tatatatcat cgcgacgaat caattttcaa
     9481 attcaacaaa atcagatctt gagaatcgag ttaataagtc tatcgaagac ttaactaaaa
     9541 tccaaccatt catgcctaca caagattttg attttaaaac taaagaaatt caatcaaaca
     9601 tttctgaaga aagatttatc gaaatgattc agtatttcaa agagaaaata acagaagggg
     9661 atatgtttca agttgtgcca tcaagaattt acaaatatgc gcatcatgct agtcagcatt
     9721 taaatcaact ttcgtttcaa ctgtatcaaa atttaaaacg acaaaaccca agtccatata
     9781 tgtattatct taatatcgat caaccatata ttgtcggtag ttctcccgaa agtttcgtaa
     9841 gtgtcaaaga tcaaattgta acaactaatc ctattgcagg tacgattcaa cgtggtgaga
     9901 cgacacaaat agataatgag aatatgaaac aactacttaa tgatccaaaa gaatgcagcg
     9961 aacatcgtat gctagttgat ttagggcgta atgatattca tagagtaagt aaaatcggta
    10021 cctcaaaaat tactaaatta atggttattg aaaaatatga acatgttatg catatcgtaa
    10081 gtgaagtcac aggtaaaata aatcaaaatt tatcgccaat gacagttatt gcgaatttat
    10141 taccaacagg taccgtttca ggtgcaccaa aattacgtgc aattgaaaga atatatgaac
    10201 aatatccaca taaacggggc gtttatagtg gtggtgttgg atacataaat tgtaatcata
    10261 acttagattt tgcattagca attcgaacga tgatgataga tgagcagtat atcaacgtag
    10321 aagctggttg tggcgttgta tatgattcta ttcctgaaaa agaactgaat gaaacgaaat
    10381 tgaaagctaa aagcttattg gaggtgagcc catgatctta gttgtagata attatgattc
    10441 ctttacatat aacctagtgg atattgttgc tcaacatact gacgtcattg ttcaataccc
    10501 tgatgatgat aatgtgctga atcaatcggt ggacgctgtt attatatctc ctggtccagg
    10561 gcatccatta gacgatcaac agttgatgaa aatcatatca acctatcaac acaaacccat
    10621 tttaggtata tgtttagggg ctcaggcact gacttgttac tacggtggag aagtcattaa
    10681 aggcgacaag gttatgcacg gcaaagttga tacactaaag gttatatcgc atcatcaaca
    10741 tctgttatat caagatatac cagaacagtt ttcaattatg agatatcatt cattaataag
    10801 taaccctgac aattttccag aagaattgaa aattactgga cgtaccaaag attgtataca
    10861 gtcattcgag cataaagaaa gaccgcatta tggtattcag taccatcctg aatcatttgc
    10921 tacagactat ggtgtcaaaa taattacaaa tttcattaat ctagtgaagg aaggatgaaa
    10981 accatgacat tactaacaag aataaaaact gaaactatat tacttgaaag cgacattaaa
    11041 gagctaatcg atatacttat ttctcctagt attggaactg atattaaata tgaattactt
    11101 agttcctatt cggagcgaga aatccaacaa caagaattaa catatattgt acgtagctta
    11161 attaatacaa tgtatccaca tcaaccatgt tatgaagggg ctatgtgtgt gtgcggcaca
    11221 ggtggtgaca agtcaaatag tttcaacatt tcaacgactg ttgcttttgt tgtagcaagt
    11281 gctggcgtaa aagttataaa acatggtaat aaaagtatta cctcaaattc aggtagtacg
    11341 gatttgttaa atcaaatgaa catacaaaca acaactgttg atgatacacc taaccaatta
    11401 aatgaaaaag accttgtatt cattggtgca actgaatcat atccaatcat gaagtatatg
    11461 caaccagtta gaaaaatgat tggaaagcct acaatattaa accttgtggg tccattaatt
    11521 aatccatatc acttaacgta tcaaatggta ggcgtctttg atcctacaaa gttaaagtta
    11581 gttgctaaaa cgattaaaga tttaggtaga aaacgtgcat cgttttacat ggtgcaaatg
    11641 gtatggatga agcaacacta tctggtgata atttgatata tgaattgact gaagatggag
    11701 aaatcaaaaa ttacacatta aatgcgactg attatggttt gaaacatgcg ccgaatagtg
    11761 attttaaagg cggttcacct gaagaaaatt tagcaatctc ccttaatatc ttgaatggta
    11821 aagatcagtc aagtcgacgt gatgttgtct tactaaatgc gggtttaagc ctttatgttg
    11881 cagagaaagt ggataccatc gcagaaggca tagaacttgc aactacattg attgataatg
    11941 gtgaagcatt gaaaaaatac catcaaatga gaggtgaata atatgacgat tttatcagaa
    12001 attgttaaat ataaacagtc acttttacaa aatggctatt atcaagacaa acttaatacc
    12061 ttgaaaagtg tgaagattca gaataaaaaa tcttttataa acgcaattga gaaagaacca
    12121 aagctagcaa ttattgcaga aattaaatcg aagagtccta cagttaatga cttacctgaa
    12181 cgagatttat cgcaacaaat ctcagattat gaccaatatg gtgcaaatgc cgtgtccatt
    12241 ttaactgatg aaaagtactt tggtggtagt tttgaaagat tacaagcatt gacgacaaaa
    12301 acaacattac ccgtattatg caaagacttt attatagacc cgcttcaaat tgatgttgct
    12361 aaacaagctg gtgcatctat gattttattg atcgttaaca tcttatctga taaacaattg
    12421 aaagatttat ataactacgc tatatcgcaa aatctagaag tgttagttga agtacatgat
    12481 cgccatgaat tagaacgtgc ctataaggtt aatgctaaat tgattggtgt aaataacagg
    12541 gacttaaaac gatttgttac aaatgtggaa catacaaata ctattttaga aaataaaaaa
    12601 acaaatcatt attatatttc tgaaagtggt attcacgatg catctgatgt aagaaaaatc
    12661 ttgcatagtg gtatcgatgg cttactaata ggtgaggcgc ttatgcgttg tgacaatcta
    12721 tctgaatttt taccacaact gaaaatgcaa aaggtgaagt catgatgaaa ttgaaatttt
    12781 gtggctttac atcaataaag gatgttacag cggccagtca attacctatt gatgcgatag
    12841 gtttcatcca ttatgaaaaa agtaaaaggc atcaaacaat tacccaaata aaaaagttag
    12901 cgtctgctgt tccaaatcat atcgataaag tatgtgtcat ggtaaatcct gatttaacaa
    12961 caattgaaca cgtattaagc aatacgtcaa ttaacacaat acagttacac ggcacagaat
    13021 ctattgattt tatacaggaa attaaaaaga aatattcaag cattaaaatc actaaagctt
    13081 tagctgcaga tgaaaacata atccaaaaca taaataaata taaagggttc gtagatttat
    13141 ttattatcga cacaccctca gtgtcgtatg gtggtaccgg tcaaacatat gactggacta
    13201 ttttgaagca cataaaagac ataccttatt tgatagcagg aggcattaac tctgaaaata
    13261 ttcaaacagt taatcaactt aaattatcac atcaaggtta tgatcttgca tcaggtatag
    13321 aagtaaatgg gcgaaaagat atagaaaaaa tgacagcaat tgtaaatatt gtgaaaggag
    13381 atagagacaa tgaataaaca aatacaaaca gaagcagatg aattaggttt ctttggtgaa
    13441 tatggagggc aatatgttcc agaaacatta atgccagcaa ttattgagtt gaaaaaagct
    13501 tataaagagg caaaagcaga cccagagttt caaagagaac tggaatacta tttatcagag
    13561 tatgtaggac gcgcgacacc acttacatat gctgcatcat atactgaaag cctaggtagc
    13621 gctaaaatat atttgaaacg agaggatcta aatcatacag gcgcccataa aattaataat
    13681 gcgttaggtc aagcgttgct tgctaaaaga atgggcaaga agaagcttgt tgctgaaact
    13741 ggtgcgggtc aacatggtgt agctagtgct acggttgctg cattatttga tatggaactt
    13801 gttgtcttta tgggaagtga agatattaaa agacaacaac ttaatgtatt tagaatggaa
    13861 ttacttggtg caaaggttgt ggcagttgaa gatggtcaag ggactttatc ggatgcagtt
    13921 aataaagcat tgcaatattg ggtaagtcat gtagatgata cacattattt attaggttct
    13981 gcattaggtc cagacccgtt cccaacgatt gttagagatt ttcagagtgt gattggtaaa
    14041 gaaataaaat cacagatatt gaagaaagaa ggtcgacttc cggatgcaat tgtagcatgt
    14101 atcggtggtg gctcaaatgc aatcggtaca ttttatccat ttattaaaga tgatgttgca
    14161 ttatacggtg ttgaagccgc aggtcaaggc gatgatactg ataaacatgc acttgcaatt
    14221 ggcaaaggat cacctggcgt attacatggt actaaaatgt atttaattca agatgaagat
    14281 gggcaagtgc aactagcaca ttctatttca gcaggacttg attatcctgg tattggacca
    14341 gaacattctt attaccacga cattggtaga gtaacttttg aaaatgctag tgatacacaa
    14401 gcaatgaatg ctttaatcaa ctttacaaaa catgaaggta ttatacctgc aattgaaagt
    14461 gcacatgcac tgagttatgt tgaaagacta gcgcctacga tgtcgaaaga agatattatt
    14521 gtagtaacta tttctggacg tggcgataaa gatatggaaa caattagaca atatatggta
    14581 gagcgaggtc ttgcaaatga ctaaattatt tataccttat attatgggca ataaagattt
    14641 gattgaaaat gcaacattgt tgagtgaaaa tggtgcagat ataattgaaa ttggagtacc
    14701 tttctctgat ccggttgctg atggtccagt tatcatggaa gcaggtcaac aagcgattaa
    14761 acaaggcatc acgatagatt atattttcaa tcaattagaa aaacatggtg atcaaattaa
    14821 gtgtaactat gtattaatga cgtattataa tattatttgt cattatggag aacaagcgtt
    14881 ttttgaaaaa tgtcgagata ctggtgtcta cggcttaatt attcctgatt taccatatga
    14941 attatcgcag cgtttaaaac aacaatttag tcactatggc gtcaaaatca tatcgttagt
    15001 tgcgatgact actgatgaca aacgtataaa agatatcgta tcccatgcgg aaggctttat
    15061 ttatactgtg acgatgaatg cgacaacagg gcaaaacggt gcgtttcatc cagaattaaa
    15121 acgaaaaatt gagtcaatta aagcgatagc caatgtgcca gttgtcgcag gatttggtat
    15181 aagaacacca caacatgttg cagatataaa agaggttgca gatggcattg tcattggtag
    15241 cgaaatcgtt aagcgattta aatctaacac gcgtgaggaa atcattaaat atttacaatc
    15301 tatccaacaa acattgaata attaagttta cttgatttaa aaaaattagg cgaatactgt
    15361 ttgaaaaagt gaaaaacggt gaattataaa attgaataga aatgatacaa aagtaatatg
    15421 agcaaaccca aacgttcata ttactttttt tgaaattgta tgcaaaaatc taaatattac
    15481 tataaaagta tacgcaatta aagcgtttat gttttagttt taacattaac tattgtatac
    15541 ttatttagat tagatttatt atttttgaca tttgcagagg ggaaatagaa aaactgcaaa
    15601 tacggaaatg aaattaatta acgagagaca aataggagta atgataatga agtttacaaa
    15661 tttaacagct aaagagtttg gtgcatttac agatagcatg ccatacagtc atttcacgca
    15721 aactgttggc cactatgagt taaagcttgc tgaaggttat gaaacacatt tagtgggaat
    15781 aaaaaacaat aataacgagg tcattgcagc ttgcttactt actgctgtac ctgttatgaa
    15841 agtgttcaag tatttttatt caaatcgcgg tccagtgatt gattatgaaa atcaagaact
    15901 cgtacacttt ttctttaatg aattatcaaa atatgttaaa aaacatcgtt gtctatacct
    15961 acatatcgat ccatatttac catatcaata cttgaatcat gatggcgaga ttacaggtaa
    16021 tgctggtaat gattggttct ttgataaaat gagtaactta ggatttgaac atactggatt
    16081 ccataaagga tttgatcctg tgctacaaat tcgttatcac tcagtgttag atttaaaaga
    16141 taaaacagca gatgacatca ttaaaaatat ggatggactt agaaaaagaa acacgaaaaa
    16201 agttaaaaag aatggtgtta aagtaagatt tttatctgaa gaagaactac caatttttag
    16261 atcatttatg gaagatacgt cagaatcaaa agcttttgct gatcgtgatg acaaatttta
    16321 ctacaatcgc ttaaaatatt acaaagaccg tgtgttagta cctttagcgt atatcaactt
    16381 tgatgaatat attaaagaac taaacgaaga gcgtgatatt ttaaataaag atttaaataa
    16441 agcgttaaag gatattgaaa aacgtcctga aaataaaaaa gcacacaaca agcgagataa
    16501 cttacaacaa caacttgatg ctaatgagca aaagattgaa gaaggtaaac gtctacaaga
    16561 agaacatggt aatgaattac ctatctctgc tggtttcttc tttatcaacc catttgaagt
    16621 tgtttattat gctggtggta catcaaatgc tttccgtcat tttgccggaa gttatgcagt
    16681 gcaatgggaa atgattaatt atgcattaaa tcatggcatt gaccgttata atttctatgg
    16741 tgttagtggt aaatttacag aagatgctga agatgctggt gtagttaaat tcaaaaaagg
    16801 ttacaatgct gaaattattg aatatgttgg tgactttatt aaaccaatta ataaacctgt
    16861 ttacgcagca tataccgcac ttaaaaaagt taaagacaga attttttagg aagggaatta
    16921 tcaaaacatg aaatttacag agttaactgt taccgaattt gacaactttg tacaaaatcc
    16981 atcattggaa agtcattatt tccaagtaaa agaaaatata gttacccgtg agaatgatgg
    17041 ctttgaagta gttttattag gtattaaaga cgacaataac aaagtaattg cagcaagcct
    17101 tttctctaaa attcctacta tgggaagtta tgtttactat tcgaatcgtg gtccagtaat
    17161 ggatttttca gatttaggat tagttgatta ttatttaaaa gagttagata aatatttaca
    17221 gcaacatcaa tgtttatatg ttaaattaga tccgtattgg ttatatcatc tatatgataa
    17281 agatatcgtg ccatttgaag gtcgcgagaa aaatgatgcc ctagtaaact tgtttaaatc
    17341 acatggttac gagcatcatg gctttacaac tgagtatgat acatcgagcc aagtacgatg
    17401 gatgggcgta ttaaaccttg aaggtaaaac acccgaaaca ttgaaaaaga catttgatag
    17461 tcaacgtaaa cgtaatatta ataaagcgat aaactatggt gttaaagtca gattccttga
    17521 acgtgatgag ttcaatcttt tcctagattt atatcgtgaa actgaagagc gtgctggatt
    17581 tgtatcaaaa acagatgatt atttttataa ctttattgac acatatggag ataaagtatt
    17641 agtaccatta gcatatattg accttgatga atatgtgtta aagctgcaac aggaattgaa
    17701 tgacaaagaa aatcgtcgtg atcaaatgat ggcgaaagaa aacaaatcag ataaacaaat
    17761 gaagaaaatt gcagaattag ataagcaaat tgatcatgat cagcatgaat tattgaatgc
    17821 aagtgaattg agcaaaacgg acggcccaat tctaaacctt gcttctggtg tttattttgc
    17881 aaatgcatat gaagtgaatt atttctctgg tggttcatca gaaaaatata atcaatttat
    17941 gggaccatac atgatgcatt ggtttatgat taactattgc ttcgataatg gttatgatcg
    18001 ttataatttc tatggtttat caggtgattt tacggaaaac agtgaagatt atggcgtata
    18061 ccgctttaaa cgtggattta atgtacaaat cgaagaatta ataggggatt tctataaacc
    18121 aattcataaa gtgaaatatt ggttgttcac aacattggat aaactacgta aaaaattaaa
    18181 gaaatagatg taaatgatgt tagggcttat agttattgat actataggct cttttttatg
    18241 ctttttttag cgaaatttta attatt
//
LOCUS       DAMZLE010000041        18162 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMZLE010000041 DAMZLE010000000
VERSION     DAMZLE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.041"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(144..458)
                     /locus_tag="POB92_001239"
     CDS             complement(144..458)
                     /locus_tag="POB92_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6578747.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            complement(615..1052)
                     /locus_tag="POB92_001240"
     CDS             complement(615..1052)
                     /locus_tag="POB92_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6578748.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            complement(1065..2432)
                     /locus_tag="POB92_001241"
     CDS             complement(1065..2432)
                     /locus_tag="POB92_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6578749.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLEDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            complement(2413..2703)
                     /locus_tag="POB92_001242"
     CDS             complement(2413..2703)
                     /locus_tag="POB92_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDI6578750.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            2837..2935
                     /locus_tag="POB92_001243"
     CDS             2837..2935
                     /locus_tag="POB92_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578751.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            complement(3044..5491)
                     /locus_tag="POB92_001244"
     CDS             complement(3044..5491)
                     /locus_tag="POB92_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HDI6578752.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSYHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(5779..5979)
                     /locus_tag="POB92_001245"
     CDS             complement(5779..5979)
                     /locus_tag="POB92_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDI6578753.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            complement(6047..6199)
                     /locus_tag="POB92_001246"
     CDS             complement(6047..6199)
                     /locus_tag="POB92_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6578754.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            complement(6196..6582)
                     /locus_tag="POB92_001247"
     CDS             complement(6196..6582)
                     /locus_tag="POB92_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578755.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            complement(6575..6811)
                     /locus_tag="POB92_001248"
     CDS             complement(6575..6811)
                     /locus_tag="POB92_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578756.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            complement(6804..7007)
                     /locus_tag="POB92_001249"
     CDS             complement(6804..7007)
                     /locus_tag="POB92_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578757.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            complement(7004..7210)
                     /locus_tag="POB92_001250"
     CDS             complement(7004..7210)
                     /locus_tag="POB92_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDI6578758.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            complement(7247..7783)
                     /locus_tag="POB92_001251"
     CDS             complement(7247..7783)
                     /locus_tag="POB92_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDI6578759.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            complement(7776..8024)
                     /locus_tag="POB92_001252"
     CDS             complement(7776..8024)
                     /locus_tag="POB92_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6578760.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            complement(8039..8281)
                     /locus_tag="POB92_001253"
     CDS             complement(8039..8281)
                     /locus_tag="POB92_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578761.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            complement(8296..8496)
                     /locus_tag="POB92_001254"
     CDS             complement(8296..8496)
                     /locus_tag="POB92_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578762.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            complement(8499..8756)
                     /locus_tag="POB92_001255"
     CDS             complement(8499..8756)
                     /locus_tag="POB92_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDI6578763.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            complement(8756..9157)
                     /locus_tag="POB92_001256"
     CDS             complement(8756..9157)
                     /locus_tag="POB92_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HDI6578764.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(9157..9342)
                     /locus_tag="POB92_001257"
     CDS             complement(9157..9342)
                     /locus_tag="POB92_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDI6578765.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(9355..11307)
                     /locus_tag="POB92_001258"
     CDS             complement(9355..11307)
                     /locus_tag="POB92_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDI6578766.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(11375..11932)
                     /locus_tag="POB92_001259"
     CDS             complement(11375..11932)
                     /locus_tag="POB92_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDI6578767.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(11958..13124)
                     /locus_tag="POB92_001260"
     CDS             complement(11958..13124)
                     /locus_tag="POB92_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDI6578768.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(13121..13483)
                     /locus_tag="POB92_001261"
     CDS             complement(13121..13483)
                     /locus_tag="POB92_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578769.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(13497..13757)
                     /locus_tag="POB92_001262"
     CDS             complement(13497..13757)
                     /locus_tag="POB92_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6578770.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            complement(13762..14064)
                     /locus_tag="POB92_001263"
     CDS             complement(13762..14064)
                     /locus_tag="POB92_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDI6578771.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            complement(14057..14374)
                     /locus_tag="POB92_001264"
     CDS             complement(14057..14374)
                     /locus_tag="POB92_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578772.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            complement(14467..14628)
                     /locus_tag="POB92_001265"
     CDS             complement(14467..14628)
                     /locus_tag="POB92_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDI6578773.1"
                     /translation="MSDTYKSYLIAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     YYKEYFYEE"
     gene            complement(14640..14903)
                     /locus_tag="POB92_001266"
     CDS             complement(14640..14903)
                     /locus_tag="POB92_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6578774.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(14928..15143)
                     /locus_tag="POB92_001267"
     CDS             complement(14928..15143)
                     /locus_tag="POB92_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578775.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            15190..15429
                     /locus_tag="POB92_001268"
     CDS             15190..15429
                     /locus_tag="POB92_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578776.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            complement(15426..15602)
                     /locus_tag="POB92_001269"
     CDS             complement(15426..15602)
                     /locus_tag="POB92_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578777.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(15686..15877)
                     /locus_tag="POB92_001270"
     CDS             complement(15686..15877)
                     /locus_tag="POB92_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6578778.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            16038..16367
                     /locus_tag="POB92_001271"
     CDS             16038..16367
                     /locus_tag="POB92_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6578779.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            16380..16841
                     /locus_tag="POB92_001272"
     CDS             16380..16841
                     /locus_tag="POB92_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6578780.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEELAHHKLTYGNILDQTKWINRKFENYAHRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            16859..17293
                     /locus_tag="POB92_001273"
     CDS             16859..17293
                     /locus_tag="POB92_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578781.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQKHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            17322..17717
                     /locus_tag="POB92_001274"
     CDS             17322..17717
                     /locus_tag="POB92_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578782.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            17807..17953
                     /locus_tag="POB92_001275"
     CDS             17807..17953
                     /locus_tag="POB92_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578783.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(17950..>18162)
                     /locus_tag="POB92_001276"
     CDS             complement(17950..>18162)
                     /locus_tag="POB92_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6578784.1"
                     /translation="IVIKDFENRIIEEGELKTYNSAGSDFDLLEVERQDFKVSDLPSN
                     DELYIKHTLVDLKQQIKLDLYLMNEY"
BASE COUNT         5407 a         3493 c         2524 g         6738 t
ORIGIN      
        1 ttttttgtta atttcatata aagaccccct ttcataaaag tttatccgcg ttgcaagcga
       61 agggcccccg ccggtacccg gcgaaaaaac attttaagcc aatgggcagg gggctataaa
      121 attttattta aatgtttttt tatttaaatt tttagaactc taattttctt aagattactt
      181 ttgtcattac catttgcatg aattttgtta tgacagccat aacaaactga cattagatta
      241 tctaattcta aagctttgtt aaaatcttca tcaacataaa taatgtgatg cactatgttt
      301 gcatctgtta caatatcttc gcgtaaacac atttgacaaa gatgattatc tctatctaat
      361 gctatctccc ttaacttctt ccatgctttt gaatgataga accaatcgta ttgatatgac
      421 ttacgaccat gcttataaat gttattgtgc ttggtcatct cttacacctc tttgattgca
      481 taacaaaaga cacaccgcat agcgatgtgc ctcgtgtact tgtgtcgtat aacttttaga
      541 taactttata catctttccg atactatcat attactacag atttgtaggc cttttgcaca
      601 atctttgcac aatgttattt gatacctgca tgatacgcta ccgctttaac aaagttcttt
      661 cgtatagtag taacagtatt acgatgcata tggcattcat gtcctatctg ttccatcttt
      721 aacttctttt ctttattcca atacttgagc cttattactt tcttatgatc ttcaggcaac
      781 tttaagtatt cactctcaac tgcttcgacc atttcttcaa ggtttcgtaa catcttatta
      841 gttaataatc tagttgccat tagttcagtt gttctaactg gttcgccttt ttgtaatggt
      901 ccatatacaa tattggaatc ttgttccttc gttggattaa gtatttccaa cctcaatctt
      961 tttatttctt tcttgttctc atttaaatta tatatttctg attcaatata tttaaatgtt
     1021 cctggcttga tatcatatat tgtgttcccc atgttagacc tccattactt atgcttagct
     1081 attcttgctt taatagcttt cattaattct tcttgcgtta gttctttatt ttgtaaagct
     1141 ttatatactc tttgatctat tgtgttatcg gtcatgatat gatgaataat agtcgtatga
     1201 ttttgtcctt gtctgtataa tctagcattt gcttgttggt ataattccaa ggaccatgta
     1261 agtccaaacc aaacaataat gtgcccacct tgttgtaagt ttaatccatg tcctgcactt
     1321 gctggatgtg ctataagcaa cttaatgtct ccactgttcc aacgttcttt atagtttgaa
     1381 tcctctaatg tggttgcttc cttaaacctt tgaagtattc tttctttatc gtgtttgaag
     1441 ttataaaaca atagtattgg ttggccttga gactcctcta taatttcctc taacttatct
     1501 aacttcttat catgtataag tcttacatct tcctcatctg tataaactgc gccgttagat
     1561 agttgaagta gtttctgact taatgatgcc ccattttgag ctacaactgt tccttcttct
     1621 tccgattcta aaatatagtt tttttctaat tcttcatata cttttctttc tttttctgat
     1681 aagactactg tttgtttagt atcaactctg tcaggcatat ccagataatc tttcgctttc
     1741 atgcttaaac atatatcttc tatttgttta tatatctttt cttcagatcc gtctcttagc
     1801 tcccacttaa aaatatgttc gctaacttga tgagttggtt taaagtacct ttctcgataa
     1861 cgactgaatg aagactcaag tctttcacct ctgtctatca aataaacttg agcccataaa
     1921 tcctgtaaac tatttggact aggtgttcct gttaatccta taaatctatt aatgagtggt
     1981 aatttctttt taatagattt aaacctttga ctcttaggac ttttaaatgt agacagttca
     2041 tcaatcacaa ccatgtcaaa tggccattct tttttatatt gatcacataa ccatttagta
     2101 ttttctttat tggttacata gatatcagcc tctgtgttta atgcatcatt tctttcttta
     2161 ggtgttccta aaactaaaga cactttcaga tgatttaaat ggttccactt atcaacttca
     2221 tcaacccatg tatctttagc aacttgttta ggtgctataa ctaacatttt tttagtgtct
     2281 aacaactgca attcactaaa tgctgtaagt gttgatactg ttttccctag acccatatct
     2341 aaaaacagac cgtatttctc attatctatc actttatcta ttgcatactt ttgatagcta
     2401 tgtggtttga agtcaatcgc caaatgttcc acctaccatt ctgataaaag tatttacttg
     2461 ttctttattc cataacacat atactttatg atctctattt tcaaattgtc tatgcacata
     2521 tttttgtaaa ggatgcaact ttcctttttc ttgcttcatt tctacaaaat atgtttttcc
     2581 ttctggcata ataataattc tatctggcac acctcttgtt ccaggtgcaa cccattttaa
     2641 acataaaccg tttagctttg atatctcttt cactaaatat ttttctaatg tcgattcttt
     2701 catatattca ccttgtatac aaaatttata tttgtgttcc gatgttgcat caattcttgc
     2761 caaactttta aaaatagctg ttagagggtt acccctatac ctctttactc cctaacacta
     2821 ctttttaaac tttatagtga atttgatgca acattggaaa caaacagggt tgaaccttac
     2881 agcgagaagg gaaagaggtg ttgtatcatt tgttgcatca atgttgcatc actaaaaatg
     2941 atgcaacacc tacgattact ttttacactc acgtgttgca tcactcaaaa aatgatgcaa
     3001 catctgatac aacactctaa aatgtatatt tattcaatat ttattatatt aaatcctcta
     3061 aactttcatc tcttacataa gctatctgta caccataatc ttttccaaat cgaattttcc
     3121 cacttttatt accatcatat acagaccaat tgtctaattg tcttaagatg tttgaaatct
     3181 ttctaatttc catagatcct ctactatctc ccttatcttt accaaaacat tcaacaaaca
     3241 cttcaagcgc acagacctta tttctttcaa cgtaatctac atttcctgtt ggtaacatat
     3301 caacatcacc ttgataaaat cgtcttcgtt caaagatagt taagtcatcc caattgctag
     3361 gaattggtgt gttaagatat tcatcaataa tgcctgtata tggagattcc tcagtatgtt
     3421 tgctttgtat tgaacgcatt tcttcttcta gttcagggtt gaggaataac tcttctcctt
     3481 gttcataata atatttagct tctgcccaaa tttggtcgat ctcttctttg gttagtttag
     3541 accagttcac ttcaactctc tctggattta cagtcattgg ccaaaaacgt cttccaccag
     3601 tttcatctct taagaaatca actttattag ttgtaccaat gaaaatacat tgccttggaa
     3661 aatcttcaat ataatgtcca taagcaacac gaaaccggtc aacttgttta gatatgaaat
     3721 gcttaatagc ttcaacttca gcttttcttg tagctgcaag ttctgccatt tccattagcc
     3781 aaacgccttg taaggcctca taggcttctt tacctgtaac agaaactaaa ctgtcagaaa
     3841 accatgcacc acctaatttt tttagcaaag cagatttacc tacaccttga ggaccataaa
     3901 gtgtaagcat atagtcaaat ttacatccag gctccattac tcgagcaatt ccagcagtca
     3961 atgctttttt ggtagttgtt ctattcactt cagtatcttc aacacctaag tatttgataa
     4021 ataacttttc aagacgttta tgtccatccc acgatatttt atttagataa tcccttactg
     4081 gatgataagc attttgcatt gctacgctta taatggcatc ttttgtttta cctgaatggt
     4141 gtatgtcata aatcttttcg atataacttc ttaaactgct atcatcaccg tcttgccatt
     4201 gacgtgtctt aaaattagta ttccatggca ctttccctaa gcattcaatt tgttttgtaa
     4261 attcattaaa tgctattttt ccttttaaat ttggatcatt acgcaatata atttctatat
     4321 ttgggatact agctttgaaa gtacctttcg aagtaatttc taacgtctca gaccatgcat
     4381 catcgctatt tactatttca tcgaaatcct gcattgcatc agacattttg tcgttaatta
     4441 attgcttttt aacaacttca tcattttgcg ctctttgctg cattgcttta taactaggta
     4501 gtcgattaac cggagtatct gttttagcgt cttcatcttg agcaccatat aagtgtatgc
     4561 gtactaaatc aaaactgttc acaagcatac cgctaacggg atccgtatta tgataagaat
     4621 aggcaaactt gttattttcg tataacacca atccacctgc agttgaacct tcatgatagg
     4681 tataacggtt agtagaatgt ttttcgtata agtcaggaat aaaagttgat atagcttctt
     4741 ctatcgtata ggctctacaa aatgcgccaa caattcccgg cttttcttct gggtcacctt
     4801 gcttatctgc taatctttta gtcttactct cttcctttga agacgttggc cattctaatg
     4861 tgtcagtcca atcaacatat tcatttaata ttttatctgg atctaacaaa ggtaaatctt
     4921 cataggtaaa gaaaaattct gcatcattgc tagttgaagg ccaatacatt aacctatgtg
     4981 gttgataagt tgtatcatcg aagtaatcca tgccaacgat atctgcgact ttacgcccaa
     5041 tagcttcata ctcatctgca tttacatttc gttttaaagg aatcactaaa cgcagtcttg
     5101 gacttatctc tctatgctta tgtgttgaat ataaacaata tgcaaaatca taaaacatag
     5161 ataatatgtc agtcatatct tgagcagcat aatcgatatc aagtgttagc attgaacgat
     5221 tcatgacttg accagcacgt cgtttgcctt cttttaaata tccaccgaca aatccgccaa
     5281 catcttttat atctgcttgt tcagacttag acattttatt gtactcagtt aaatcttctt
     5341 tagttctaac tgtttgtgct agcttctgca taaagtcaga ccaagccata ttgtgattag
     5401 tccaatatgt ggataaacga ctagcagcat aagaatatga gacatcacga tcatatttaa
     5461 ttgtttctat ttgagtgact ttgtctaaca tattcggctc ctttcttgta taattaattt
     5521 aataaaatta aagaggtgac aaattttgaa aactttacgc gaaatcattt atgataaata
     5581 cgaaaatgaa aacttgagaa ctttaatttt attgcactca ggaaaagcct ttcccgtaac
     5641 taatataatc tcagaaaatt caaccttctt aattatcaag acttcaaaga caatttcaca
     5701 taaattagtt ttaatttaca aaaacgaaat aaaaaatatc cgcttctttt aatcctggag
     5761 agggctttag ccccctcttt agcttttgaa tctttttcta attcgttcaa cttcattttc
     5821 ataatcttct aaaccttcaa caccattatt ttttactaac tgcttgaaaa gataagaatt
     5881 catatactcc aatgcttcta tggttttcat cttatgagaa atgctactta acaagatcaa
     5941 taaaaatata gctaaaacga ttgaaatgac aatccacata tttacaacac ctccagtgct
     6001 attgctaaac acattaatat aattaattca aaaatgataa tagctattac catgaaactt
     6061 cagctctgat tttttcgtaa tcactcggcg cctctacatc atcattagcc gtcatcataa
     6121 tatacacttg ctcagttaca tacttaccta actcatacat tgctagtaag aataatagtc
     6181 ttagtatttg tttaatcatt gtttatctac cttctttact tcgtataaga ccggatataa
     6241 atttaaaaag tgtattctat aaccgattgt tttaacttct accttatcgc ctacttttaa
     6301 cctagcttgt atatctgcgc tatcaaattt ctttttgaat aataagtcgg aattttcaat
     6361 gacttgtttg ttgtctaata caatatagaa cttgtcttct ttatcttgtc tcttgttata
     6421 tttatctgta atagttcctt gatgtaattc tttgtgttgg taactagcca ctgtatagat
     6481 aggcaatgtg ataacaagta gcaatgcgaa tataccgaat aatgacagta ttccaacaat
     6541 aaagatgtcg aacccatcca tatttttaag ttttttaatc atcattgtca tctccagtat
     6601 caattaaact aggcatcatt cttaacatag cccttaattc atattcattc atattagcca
     6661 tcgtaggact gtaaaattca ctgtctttat ctttaatttc tttaataaaa tcatcttcaa
     6721 tcttagcctt ttcttcaggt gctttatttt tatatttttt gattatttca gtgtactttt
     6781 tcgggaattt cattttaggt atgttaatca tcgtttgcct ccttaataaa tgtaaatgat
     6841 tcaatctcat ctcttttaac ccatacttca ttgttgaaca catctttgac cggaagaaaa
     6901 tcctcaatca ctagattcat aacaagatta atataatcgt cagaagctag atctgttgtt
     6961 gtgtaataaa ctctatctga aatagtttta attttaacct ccgtcatttc ccacactccc
     7021 ttatattttc aaataactga cccactttaa taactgcatc tcttttaact tgtgcctcgt
     7081 atttgcgctc agcttcttct ttactctctg cctcaacaac tgtaaacctt tgattgcttt
     7141 tagctttagt tatgtgtgta tgcttgcgtc ctgttgaatc tttgaatgtt gtgactaagt
     7201 attgcgtcac ttccccaaaa cctccttgac tcgatctaag atgtctttac acgtatcctt
     7261 ttcctgcgtc tgctgttcca tcttgtcttt catgattcct tttcattttc tttttgtatg
     7321 cgtcaatgag ctggtcgata gaatataagt tgaaagctat gtctatcgct attacaattg
     7381 ccaattggtc aggataaaat tctttgaata ttatctgtgg tgtgctaaca actgcgtctt
     7441 gagcaaattc tttatcttta aaattaaaca tgttgtgaaa ttcactattt ttaaaacttg
     7501 attcaatcgc ttcttttatc tcttctgatg acactcctac ttgattcgca atactcaatc
     7561 caaacgccaa catgtccgct aattcatcta actgaacatc taacggctta cctggtttct
     7621 tcttccagtt cttaaacgtt tccaatgtat taaaccattc aaagaattca actacatatg
     7681 ctattttgct atctcgtaag ttcagcgttg gtattctatc gtcgaactcc ttttgtattt
     7741 gtaataactc ttgtaactga tcaattgtta atgtattagt cattttcctg atcctcctca
     7801 tatttataga ccacttgccc cgtcataatc cctactgctt catcaagacc aatatcttct
     7861 ttgagtgcat cttgcatagc attaggtaaa ccttcaagta tttcatcaaa cgcttgcgct
     7921 ttcttataca cgtcttcaat ctcttttagt aatccctctg tgtcattgcc gttatacgca
     7981 ctagcactga taattgattg ttcaatttgt tcgcggttat tcatcatttc catctcctct
     8041 aaaataaagt tagttgcttc tgttcctcgt attccaaacc atgttgcttt atatatattt
     8101 cgagctcttc agcagtatca aatgtctttt taacgctttg ccaacctggc acgatatgcc
     8161 catgaaagta atacgtgcca tttactacat gagtatgtgc cactcgttcg ttatcctgat
     8221 acagatatct tttagagccg aaaaattggt ttaagcgttc tttacgtgcg ctatctgtca
     8281 tggtcgtcac tccttttaac aattaggcag tccaaacgac atgcattcgt aatatagttc
     8341 ttcattcctt atgcttgtct tatagttctc aatcacattg ctaacttctt tatgactcat
     8401 tgctttaact tgttcgtctg tatatttttc gcagtcttct aattccagtt gctcctgtaa
     8461 tgacattacg taatcaactt gtttttgcgt tgccatcgtt acccctccca caagtcaaac
     8521 actctatcga cgtaaaactt cgcctttgct aaatcctcat gaccattctt taacggtgct
     8581 ctagacaagt atttgattgc attacctatt gcgaatgcta attgtggtgg atactgtgcc
     8641 gtaacttgtt cgatgaaatc tataatttca atgtcgccgt atgtgtaatg cgcaggttgt
     8701 ttaacattgt cttgtgtttc attcatatct acttttctgt tactgattat gctcattatg
     8761 cttcactcca tttcttgaac atttggttat aagtgacatc gaaccagtac ggatcacgtg
     8821 aatgtttttg aggcgttcca tcataaagcc atggtctcaa tcttctcttt ctttcttctt
     8881 catattccgc tctcacattt cgttggtata ggttcaaaat cgcttttttt ctgatttttt
     8941 ctctctcttt ttcttcatct tttatttgac tcttcatata ttcaacttca tctttagatt
     9001 ttgagtcttt tcttccacac aataattcat cgccgcgcat tttatgtttg tatctgtatc
     9061 taagaagttc tggagatata tgatattttt ctgaaacttc tctcaatgtc attagttttc
     9121 ctttgatacg cactcttata acttttcttc tagccatcat tccacctcta aatctaaaac
     9181 cttgatattt ataacgttat attttaatag ttcacctgga ttattaaata aatagtccgc
     9241 caaattctct ttttctttat caatctgatt gtaattaaca ctttcgactt ctgtaggaat
     9301 tctaatgtca acagaagcat tgatataagc ttgatgttgc attcaatcac actcctaatc
     9361 cttcatataa aacggagaag taaatccgtc actattcaaa ttcaatcctt ttgcccaatc
     9421 gacaggctta ttcatgatag tttcgatttc cttaagtcca tttgaacctc taggtatttc
     9481 tacaattact tcatcatgga catgtccaac tattttaaaa cctgatgctt caagcctagc
     9541 tatagaaatc gcaagtaaat cccttgcagt tgcttgaaca atattctcga ctaacttccc
     9601 accatacgtt tttaactttg accatttacg gttaagatct aagcccataa attcaacaac
     9661 ttgactaccc caactatttt caccaactga agcttttgga taagctaaag ctcttccact
     9721 aggcagttca atcattagaa aacctttttt catataaaat ctaactccat gcgtatgatg
     9781 cgtctttcgg gatttcacag tattaattgc agcctcttgg caagccttcc aaaaattaac
     9841 tatgttagga tttgcgttac gccaactatc aactaaacct tgtaattcat tttcttcaat
     9901 gcccatttcc aatgcgccca tcgcttttaa agctccagcg ccaccttgat aacctaaagc
     9961 taattcggac acttttccct tttgtctgag agggtcgcct ttagttatgc tttctaccgg
    10021 gacattaaac atttgagaag ccgatgcttc atatatcttt ccgtgtgtgt tgaacacatc
    10081 taaacgccat tgttcttttg cataccatgc tatgactctt gcctctattg cagaaaaatc
    10141 acttactgct agttcattac cttcttcagc agtaaatgtc gtcctaacta attgacttaa
    10201 taagtcttga ggatgaacat tgagtaataa atctaaatcg tcaaaacgtt gttctttaat
    10261 aagatctctt gctatttcta attcagtatc tgaaatataa tgctttgtta aattctgaag
    10321 ttgtacacct ctacctgccc atcttccagt accggcaccg taaaattgaa acagacctct
    10381 tacccgttca tcactgcaca tcatgtcatg cattttgttg tattttttca cactggtttt
    10441 agacatttgc aatctaattt ctagcatttt tttagctttt cctgttgctt cttttaagta
    10501 ctcctgaacc gttttctttt gtaaattagg tatatctaat ccttggtcat cctttaacca
    10561 agccaataat tgtgtaggac tattaggatt ttctaaacct gttatatgtt tagcttgatt
    10621 aagcaattct tctttactct gcttatcgag cacattagct cctaacatca atgatttaga
    10681 aagcttaata cctctgtcgt ttatatgttg gtcaaaaacc caatatgctt gttcaattgc
    10741 agttactgga aagtctttaa ttttatgagc aatcgtcatt tctacttcta catctcgaat
    10801 acagtaatct ataaattgtt gccatttttc aagatcatgt tcaggcaagt ttcttgttct
    10861 tcctccatta acttttgttg gtttacaagg tatagagaaa taacgaatta aatttttacc
    10921 tgctttatct ttttggtttt gtagtcttaa aacttctcca actttatcaa gcgaagcagg
    10981 taagccaata cgcattgaat taaccattgt gcaaatccat tcttcaggtg gcatctgttt
    11041 attaaaatgt ttagcaagac aagttctttc gaaattagca ttgaatgcat acttttttac
    11101 agcaggatca aaaagagcaa ttttaaacgt ctcaaaatca gcgtggaaag gctcattatc
    11161 tactttagtc atgtcaatcg cactaatcgc tccaccatct attgaataag ctataattaa
    11221 aatttcgaaa tcttcagctt ctgtgtattt ataggcacca catttcgaaa tatcattact
    11281 gctatatgtt tcaatatcta tattcataaa tctcaaattc ttgacacctc aatttcttta
    11341 aaattaaagt ggggctaaaa ccccacctat tgacttataa gaaatcctca tcatcagtgt
    11401 ctaattcatc gaaatcatct tctgctgcac ttgcaccgcc aagaggttcg cctttttcta
    11461 caagttgaat gttgttcaat ccaactgcga tacccttatt accatttgtg ttgaaaggaa
    11521 atagattaat tgaagctcta atataatcac cacttacaac agttccagaa tccgttaatc
    11581 taattttgtt ttggtcaata ataccaggtg cttgtttgct tgatgcgtta ataaaataag
    11641 cgtcttgata attgacatca tcttctcttt cagtatctcc atcacgtaat ggaagtttca
    11701 gatttgcagg aactttgcct ccaaacttac taacttttcc ttcttcttta gcagcttcta
    11761 tagcttgttc aatggctttt atcgtacttg tatctgattt aggaatgatt aaactgattg
    11821 aatactttga ttcttgccct tcttgcatac tgtgaggttc aaaaatatgt gcatatgatg
    11881 ctcttacttt tcctgtaatc actttagttt tatttaatac ttttgctttc atgtttatat
    11941 accgtccttt taaattttta tagtttgtca aaatcatctt cagcagattg ctttatagct
    12001 ggtcgtttat ccgactcggt agcaagtgtt aatttacctt gcggcttttc tataaaaccc
    12061 tctgtaattt tagaaaatgc ttttttacca attaattttt ctaaattcgt aatgctaagt
    12121 aacttggttt ctgtaatatc ttcaggttta taacccgctt caactaactt ttcaagcatt
    12181 gcttttgtat cagttatcat tcttcgtgaa cgaccttcta caagcttcca accaggatag
    12241 tttttatcat tttctttcgc ttgatttagt gcatattgtt ctacttcatc agcccatttt
    12301 ttgatatcag gcagtttata taaaagttct gcaatctctt catcacttaa caaatgtggt
    12361 ggcttttgag gcacattttg catgtattct gcacgtgttc tacatgaatg atttatctta
    12421 cagaatctac aatgactacc tgctttaaac tctcctccac cgttataagc aagtctggct
    12481 aatggtttaa caaaatcggc tccccattga agtaatcttg atattggtaa ctcttcagta
    12541 gaaaagttat ctattcgagg ttgtatgata gtcatgcgaa ttgtatgaat gtcatacatt
    12601 aaactaagca gttcatatgc gcccaagcca tataatctaa gttgaggatt atctatagct
    12661 gaaacttcaa tgcctttacc atatttaagg tcaataattt caagtacacc acctgaaaat
    12721 ataatgacat caccagttcc aaaagattca gggacgtatt tacctaaatc taattttgtt
    12781 tcaaataaag ctattacatc attatcccta ctcaaagctt cgttatattt ttcttctaca
    12841 ttagctacat actcttccac atattcacgc aattcttcgc tgtaatattg atttctctta
    12901 taattttgaa aagccttatt aaactcaaac tgtgttaggc cttcatattt aaggctgaaa
    12961 tataactcac ttaactcatg ggcgaatgta ccttcttcgg caaaaactgt acttttatct
    13021 gcaatacctt cacttgcctt aatactcggt gggcagttta gccattgttt tgctccactt
    13081 gcactaagct ttgcatgagc tctatttgag tgatctagct tcatgcattt attctcgcat
    13141 tcataaaatc aacaattttt tcataatgct cttctttgat agtagatagc ttatccgcac
    13201 caagttcgtt aagtttattt ctaaattctt tcttatcaga agtatctgct tttttaagga
    13261 actcttttcc tactgataaa acataatctt tagttaaatc agcagaagtt tccttaactt
    13321 cttcaattga ttccagttga gctgtttctt cttttggcat tggtgcttct ttaactttct
    13381 cttgtacaat tgatgaatct actgttgata gttcagtgtt taacacacgt aaattgttat
    13441 ttaatagttt taattcttca aaaatatctt ctaatattgc cattgattaa atcctcctac
    13501 catttcatga ctaagttaat tagtctgtcc tgttcatctg tgttctcttc aatccattcg
    13561 tttataacgt cacgcattgc atccgtcgca aaatatagtt cgcttaaatc tacaacatga
    13621 aatgatttaa gtggaatatt attcatatcc ttgatttgta tactgatacc gtcatgtttt
    13681 ttcatcgcag acactttaaa ttcaaacccg ttaaagctga taattttatt ttttatctca
    13741 ccaaatttgt aatacattgt tttagccctc cttgttatca tcaataccgt gaaatttttg
    13801 tgatttacac atttggagaa cattgacaat gtctttataa ctcttagtgc tatccaataa
    13861 ggaagcaaga tcgaaagtat gaccaatcac agaacttgaa cctgctaaat aatctccgtc
    13921 gataactcct attgatgaga aaagcaaaat atcaaattta ctttctccct taatttcttt
    13981 cgctaattca tacaattctc cgcttttttc agataataag tcttttactt cttcctgagt
    14041 catgtcttta tattttttag tcattgttga cttcctcctc gttttcttcg tcctcctcgt
    14101 tatcttcttc gttttgtaat tcataaattt tgttttttag ttttatattt tctttttcca
    14161 atttttcgtt ttttctttct tccgcaaaat acttacctct gtaagtatct tcttctttat
    14221 ctttaacagc ctttatttca ataagttttc tgtactcgtt caatgtgatt gttactgtca
    14281 attcttgatt tgctacaaag ttatcttctt catttctgta tcctgagaaa tctttagtgt
    14341 aataatgttg ttcagtttta atattttcag ccatagttga ctacctccgt atattttgat
    14401 ttaattaagt tgtatatttt gataaatgtt tgtcactgtt acttgttgac gcaagtagca
    14461 gtttttttat tcttcataaa agtattcttt gtagtatatg aatgttgcga tacttgcgaa
    14521 tcccgcaatt gaccatgctg tagtgaagta cagcaatggc ataagcacaa tcgctaagac
    14581 tgtgaagcac agtactgcta ttaagtagct tttatatgtg tcgctcattt aatatcctcc
    14641 taataccatt ttttatgctt tctgatcaaa tactcttcca atttagaaat attaatcaga
    14701 gtgcctgttg gtgaataatc aatgtataaa ttttctacac ctaaattatc tttgcggtaa
    14761 tatttcaacc agttgtatac tgtacttcta catactccaa acaattgatg gatttgtgta
    14821 ggtgttgcgt ataacttttt cacaaatttt tcttcgcctc tatatgtgtt ttctggtgtt
    14881 ggtggtacta tgatttttgg catctctatc actcctttcg ataaatgtta aagtttgtta
    14941 ttattcgctc tgtattgaag ttctctatct aatgcataga aaactttgtt tatttctaag
    15001 tagctgtaat cacctttttt aatacttcct aatatttcct ttcttagtcg acgttcattt
    15061 tctgttaaag attctactga cgcatgatct cttctgaaaa tccttggtat gattatgtct
    15121 aacccttctg attttttgtt catttgttgt tccacctttc gtgtataatg ttgttatcaa
    15181 cctaaggagg tgataagtat acataagaga ttgctcactc aatatttaga taaagaaatc
    15241 gttacttctt tagatttaca tttaattaat ggtgaagtta ttaaagtaca agaacatata
    15301 aaagatgctg aaagcaaaac tctacacatc attcatccaa aagatagagt tgtcagttta
    15361 gatcatgttt tgtatttcga cattaacgtt aaaggtgaaa agaataacga tagtccttat
    15421 ccatcttaaa atccatagtg cttatagtta tttgctattt gttgacagtt atcactggca
    15481 agtcctacct tgtcagcttg gtaactgtat tgattattta cttcatttac taatcgattc
    15541 cattcgtctc ttggtatatc ttcaagaagt aagagaatca tctttaattt ttcttcgttc
    15601 atttgttgtt cctcctttta agttgtttat gtccatttgt tacattgcct aaaagttata
    15661 aaaagttata cgaaggtcaa aaaatttaga cctttatctg gtctacctca atatcatata
    15721 actttgctaa agcgtaaact gtaacattac ttaaattagt gtcatccttt tcccaatgac
    15781 caactgtctt tgcattgacg cccaatatat ctgctacttc ttgctgtttc aagtccctta
    15841 caagtctcca ttttcttacg gataattgtt ccggcatgtc gtccacctcc ctttcacacg
    15901 tataacttta tattactttg tattacttgt caacagataa agttatattt cttcataaaa
    15961 agttataaaa agtattgtaa agtaatgtcg gttatggtaa gttaatatta cattaagtaa
    16021 tattaaagga gacaactatg gagaataata aagtcagaaa aattttatct gaaaaccttc
    16081 aagaacttat gaatgataaa aatattgatc agagagaact tgctgaagct attggagttt
    16141 ctcaacctac agtctccaat tggattcaac aaactaaata tccacgaatt aaaagaattc
    16201 aacaacttgc agattacttc aatgtaccga aatcaagaat tactgaatca aaaaaagata
    16261 tacatcaaga aacaattgct gctcattttg ataaggaggg attaactgaa gaagagattg
    16321 aagaagtaaa tagattcatt gaatgggtta gaaatagaga caaataaagg gtgtttatat
    16381 tgggattata cgaagaactt tgcataaata acgaaaaaat aaagatagaa gaaactgacc
    16441 agcttccaaa tttccaacct ggatgctata tgaacggaaa aatttatata aggcgtaatt
    16501 tatcagaagt acgtaaagca gaagtgttat atgaggaact tgcccaccac aagttgacgt
    16561 atggcaacat tttagaccaa acaaaatgga ttaatagaaa atttgaaaat tatgcacata
    16621 gacatggttt tacttcagct gtaccgctac atgaaattgt agaagctcac aactacggtg
    16681 ttcgtaattt gtacgagttg tcagagtatc tgcagttgag tgaatcatac atactagaag
    16741 ctatagaaca atataaaaag atatatggta ttggaactca ctatggcgag tattctatta
    16801 cgtttgagcc gttgagagtt tttaaatata aggaaatata aacaaaggag agatacatat
    16861 gaaaaaagta atcggactgc tactagtaag tacattagct ttaacagctt gtggtgaaaa
    16921 agaaaaacca aaaaaagaag aaaataaaaa gtcacaaaca caaaaacaca aagatagcaa
    16981 accaaaaacg caacaagaaa aaatgaaaaa agttgaagat aaaaatccac ctaataatag
    17041 catacaaaat aattcaaaca atcaaaacca atcacaaaac aatcaactta ataataattc
    17101 agatccatct aataatactc ctgcaaatat aaatgaaaac gattcacaaa atactaattt
    17161 aaatgatgag tatgtcgttt cgcctggctg gactaaagat gaacaggcta aagcttttga
    17221 agagtacaaa aaaggaaaag aagaggaagc aagagctggt gctagcgcag taccaggagc
    17281 caatattaac taataaaaca atataagaaa gaagagctaa tatggaaaca aataaaacaa
    17341 tcgatttaat gaattatgtg gaatttccaa aaagatacac agaggcaaaa ggcaaattag
    17401 ttgcacaacc aataactact ataaatagcg caagaagagt tgaaaatgaa gatatgactg
    17461 tttgctacat tttagatcag gatgatgatg taatggactt tatctttgac agagatataa
    17521 ttactgttta ctgtcctgaa aacggaactg cgactgatga atatttttgt gaaattatat
    17581 ttaactcaga tgacacattt accctaaagc gattatctaa ttacgttacc attaaagata
    17641 gaagctaccc aatgtcaaaa ataaatgacg taaacattac gggcaaagtc gtcagattat
    17701 ttagagattt taaataaact tggctttaat tacgattaaa agtacctata tagcgtgacg
    17761 agaaaaagga ttaaaaaaaa tcaaaaaacg cctactagtg tagacgttga atggtggtga
    17821 gaattttatg gtagataaaa acaaaaaaca agaaactact cgtagtaacc cattaaacaa
    17881 aagttttgaa aagtcaggcg ccagcgaaaa attaaaaagc actttatcag aaaaagctaa
    17941 gaaaaaagat tagtattcat tcattaaata taaatccaat ttaatttgtt gtttaaggtc
    18001 tacaagcgta tgtttaatat acaattcatc gtttgacggt aaatcagata ctttgaaatc
    18061 ttgtcgctca acttctagta aatcgaaatc gctaccagct gaattatagg ttttaagttc
    18121 accctcttca atgattctgt tttcaaagtc ttttataact at
//
LOCUS       DAMZLE010000042        17915 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMZLE010000042 DAMZLE010000000
VERSION     DAMZLE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.042"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            420..818
                     /gene="nrdI"
                     /locus_tag="POB92_001277"
     CDS             420..818
                     /gene="nrdI"
                     /locus_tag="POB92_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI6578785.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            781..2886
                     /gene="nrdE"
                     /locus_tag="POB92_001278"
     CDS             781..2886
                     /gene="nrdE"
                     /locus_tag="POB92_001278"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI6578786.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            3004..3975
                     /gene="nrdF"
                     /locus_tag="POB92_001279"
     CDS             3004..3975
                     /gene="nrdF"
                     /locus_tag="POB92_001279"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI6578787.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            4489..4644
                     /locus_tag="POB92_001280"
     CDS             4489..4644
                     /locus_tag="POB92_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578788.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            4683..5654
                     /locus_tag="POB92_001281"
     CDS             4683..5654
                     /locus_tag="POB92_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6578789.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5641..6597
                     /locus_tag="POB92_001282"
     CDS             5641..6597
                     /locus_tag="POB92_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6578790.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVIFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            6594..7355
                     /locus_tag="POB92_001283"
     CDS             6594..7355
                     /locus_tag="POB92_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578791.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            7474..8502
                     /locus_tag="POB92_001284"
     CDS             7474..8502
                     /locus_tag="POB92_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6578792.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(8819..9133)
                     /locus_tag="POB92_001285"
     CDS             complement(8819..9133)
                     /locus_tag="POB92_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578793.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(9151..10074)
                     /locus_tag="POB92_001286"
     CDS             complement(9151..10074)
                     /locus_tag="POB92_001286"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI6578794.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKTTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(10201..10719)
                     /locus_tag="POB92_001287"
     CDS             complement(10201..10719)
                     /locus_tag="POB92_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDI6578795.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFHTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            10839..11717
                     /locus_tag="POB92_001288"
     CDS             10839..11717
                     /locus_tag="POB92_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDI6578796.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            11871..12191
                     /gene="ytxJ"
                     /locus_tag="POB92_001289"
     CDS             11871..12191
                     /gene="ytxJ"
                     /locus_tag="POB92_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDI6578797.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            12616..13740
                     /locus_tag="POB92_001290"
     CDS             12616..13740
                     /locus_tag="POB92_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6578798.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(13922..15148)
                     /gene="pepT"
                     /locus_tag="POB92_001291"
     CDS             complement(13922..15148)
                     /gene="pepT"
                     /locus_tag="POB92_001291"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI6578799.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(15162..15656)
                     /locus_tag="POB92_001292"
     CDS             complement(15162..15656)
                     /locus_tag="POB92_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI6578800.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(15674..16435)
                     /locus_tag="POB92_001293"
     CDS             complement(15674..16435)
                     /locus_tag="POB92_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6578801.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(16631..17701)
                     /locus_tag="POB92_001294"
     CDS             complement(16631..17701)
                     /locus_tag="POB92_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6578802.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
BASE COUNT         6349 a         2577 c         3123 g         5866 t
ORIGIN      
        1 atagtgtgtc ttatgtataa tgaaacacat aatatagtgt tggtgaaacg aaaaagacac
       61 aatatcttgt gttttgtatg caaatgcttt atttatgaag aaattacatt taaaagtaat
      121 ttaacacaga aatttaatag ttattatcaa ttaatagtca tatttttaga aaatgtactg
      181 agcaaatgga agatatccaa tgatgtaaat actacatata gtgattttta tacattcaac
      241 ccatataagc tactattttc tcaaatataa atctatgcaa ttggtttaca tttgagaaaa
      301 taagtagctt cattatagtt aatacaatgc tgagataacc atagtaacca tgttgttaaa
      361 gcatttttta attggaatga ctactttatt taaaagggtt gaagaaagaa ggtgatccaa
      421 tgaaaataat atatttttca tttactggaa atgtccgtcg ttttattaag agaacagaac
      481 ttgaaaatac gcttgagatt acagcagaaa attgtatgga accagttcat gaaccgttta
      541 ttatcgttac tggcactatt ggatttggag aagtaccaga acccgttcaa tcttttttag
      601 aagttaatca tcaatacatc agaggtgtgg cagctagcgg taatcgaaat tggggactaa
      661 atttcgcaaa agcgggtcgc acgatatcag aagagtataa tgtcccttta ttaatgaagt
      721 ttgagttaca tggaaaaaac aaagacgtta ttgaatttaa gaacaaggtg ggtaatttta
      781 atgaaaacca tggaagagaa aaagtacaat catattgaat taaataatga ggtcactaaa
      841 cgaagagaag atggattctt tagtttagaa aaagaccaag aagctttagt agcttattta
      901 gaagaagtaa aagacaaaac aatcttcttc gacactgaaa tcgagcgttt acgttattta
      961 gtagacaacg atttttattt caatgtgttt gatatttata gtgaagcgga tctaattgaa
     1021 atcactgatt atgcaaaatc aatcccgttt aattttgcaa gttatatgtc agctagtaaa
     1081 tttttcaaag attacgcttt gaaaacaaat gataaaagtc aatacttaga agactataat
     1141 caacacgttg ccattgttgc tttataccta gcaaatggta ataaagcaca agctaaacaa
     1201 tttatttctg ctatggttga acaaagatat caaccagcga caccaacatt tttaaacgca
     1261 ggccgtgcgc gtcgtggtga gctagtgtca tgtttcttat tagaagtgga tgacagctta
     1321 aattcaatta actttattga ttcaactgca aaacaattaa gtaaaattgg gggcggcgtt
     1381 gcaattaact tatctaaatt gcgtgcacgt ggtgaagcaa ttaaaggaat taaaggcgta
     1441 gcgaaaggcg ttttacctat tgctaagtca cttgaaggtg gctttagcta tgcagatcaa
     1501 cttggtcaac gccctggtgc tggtgctgtg tacttaaata tcttccatta tgatgtagaa
     1561 gaatttttag atactaaaaa agtaaatgcg gatgaagatt tacgtttatc tacaatatca
     1621 actggtttaa ttgttccatc taaattcttc gatttagcta aagaaggtaa ggacttttat
     1681 atgtttgcac ctcatacagt taaagaagaa tatggtgtga cattagacga tatcgattta
     1741 gaaaaatatt atgatgacat ggttgcaaac ccaaatgttg agaaaaagaa aaagaatgcg
     1801 cgtgaaatgt tgaatttaat tgcgcaaaca caattacaat caggttatcc atatttaatg
     1861 tttaaagata atgctaacag agtgcatccg aattcaaaca ttggacaaat taaaatgagt
     1921 aacttatgta cggaaatttt ccaactacaa gaaacttcaa ttattaatga ctatggtatt
     1981 gaagacgaaa ttaaacgtga tatttcttgt aacttgggct cattaaatat tgttaatgta
     2041 atggaaagcg gaaaattcag agattcagtt cactctggta tggacgcatt aactgttgtg
     2101 agtgatgtag caaatattca aaatgcacca ggagttagaa aagctaacag tgaattacat
     2161 tcagttggtc ttggtgtcat gaatttacac ggttacctag caaaaaataa aattggttat
     2221 gagtcagaag aagcaaaaga ttttgcaaat atcttcttta tgatgatgaa tttctactca
     2281 atcgaacgtt caatggaaat cgctaaagag cgtggtatca aatatcaaga ctttgaaaag
     2341 tctgattatg ctaatggcaa atatttcgag ttctatacaa ctcaagaatt tgaacctcaa
     2401 ttcgaaaaag tacgtgaatt attcgatggt atggctattc ctacttctga ggattggaag
     2461 aaactacaac aagatgttga acaatatggt ttatatcatg catatagatt agcaattgct
     2521 ccaacacaaa gtatttctta tgttcaaaat gcaacaagtt ctgtaatgcc aatcgttgac
     2581 caaattgaac gtcgtactta tggtaatgcg gaaacatttt accctatgcc attcttatca
     2641 ccacaaacaa tgtggtacta caaatcagca ttcaatactg atcagatgaa attaatcgat
     2701 ttaattgcga caattcaaac gcatattgac caaggtatct caacgatcct ttatgttaat
     2761 tctgaaattt ctacacgtga gttagcaaga ttatatgtat atgcgcacta taaaggatta
     2821 aaatcacttt actatactag aaataaatta ttaagtgtag aagaatgtac aagttgttct
     2881 atctaacaat taaatgttga aaatgacaaa cagctaatca tctggtctga attagcagat
     2941 gattagactg ctatgtctgt atttgtcaat tattgagtaa cattacagga ggaaattata
     3001 ttcatgatag ctgttaattg gaacacacaa gaagatatga cgaatatgtt ttggagacaa
     3061 aatatatctc aaatgtgggt tgaaacagaa tttaaagtat caaaagacat tgcaagttgg
     3121 aagactttat ctgaagctga acaagacaca tttaaaaaag cattagctgg tttaacaggc
     3181 ttagatacac atcaagcaga tgatggcatg cctttagtta tgctacatac gactgactta
     3241 aggaaaaaag cagtttattc atttatggcg atgatggagc aaatacacgc gaaaagctat
     3301 tcacatattt tcacaacact attaccatct agtgaaacaa actacctatt agatgaatgg
     3361 gttttagagg aaccccattt aaaatataaa tctgataaaa ttgttgctaa ttatcacaaa
     3421 ctttggggta aagaagcttc gatatacgac caatatatgg ccagagttac gagtgtattt
     3481 ttagaaacat tcttattctt ctcaggtttc tattatccac tatatcttgc tggtcaaggg
     3541 aaaatgacga catcaggtga aatcattcgt aaaattcttt tagatgaatc tattcatggt
     3601 gtatttaccg gtttagatgc acagcattta cgaaatgaac tatctgaaag tgagaaacaa
     3661 aaagcagatc aagaaatgta taaattgcta aatgacttgt atttaaatga agagtcatac
     3721 acaaaaatgt tatacgatga tcttggaatc actgaagatg tgctaaacta tgttaaatat
     3781 aatggaaaca aagcactttc aaacttaggc tttgaacctt attttgagga acgtgaattt
     3841 aacccaatca ttgagaatgc cttagataca acaactaaaa accatgactt cttctcagta
     3901 aaaggtgatg gttatgtatt agcattaaac gtagaagcat tacaagatga tgactttgta
     3961 tttgacaaca aataacaatt aaattaaaag accttcacat gtaaagggaa atagcgattc
     4021 gtttcgtctt gtctcctaca tgttgaaggt ctttttttat gtgtatctaa ctcattatga
     4081 gtctgagtaa gaaatcaatg ctctaagatg tacaatgtta tttatattgg cagtagttgg
     4141 cggggcccca acacagaagc aggcggaaag tcagctaaca atattgtgca agttggcggg
     4201 gccccaacat agaagcagac ggaaagtcag ctaacaataa tgtgcaagtt ggcggggccc
     4261 caacataaaa gcaggcggaa agtcagctaa caatattgtg caagttcggg cggggcccca
     4321 acataaagaa aaactttttc ctttagaaat tatcacttcc acatgagttt tactcatgta
     4381 ttcctatttt taagtacaca ttagctgagg ctaatgttaa gaaccactac ataatcaatc
     4441 attagtagtt tttatcattt ccactattcc cagacatcaa aatcttaagt gttctatttt
     4501 actttaagca aacaaaatac acattccgaa aaattaaatt tcagtttaat tgcaaatatc
     4561 aataaaattg acactaaatt atttgaaagg ctattgaaat tatggtcaaa aaacgctact
     4621 attaatgaga aatattatca atgataatga ttatcattaa tttaaaggga gaaaaatttg
     4681 taatgaagta tttattaaag ggaaatattt tgcttctatt actaatattg ttgacaatta
     4741 tttcgttgtt cataggtgtg agtgaactat caattaaaga tttactacat ttaactgagt
     4801 cacagcggaa tattttattc tcaagccgaa taccaaggac gatgagtatt ttaattgctg
     4861 gaagttcgtt ggctttagca ggcttgataa tgcaacaaat gatgcaaaat aagtttgtta
     4921 gtccgactac agctggaacg atggaatggg ctaaactagg tattttaatt gctttattgt
     4981 tctttccaac cggtcatatt ttattaaaac tagtatttgc tgttatttgc agtatttgcg
     5041 gtacgttttt atttgttaaa atcattgatt ttataaaagt gaaagatgtc atttttgtac
     5101 cgcttctagg aattatgatg ggtgggattg ttgcaagttt cacaaccttc atctcattgc
     5161 gcacgaatgc tgttcaaagc attggtaact ggcttaacgg gaactttgcc attatcacaa
     5221 gtggacgcta tgaaatttta tatttaagta ttcctctttt agcattgaca tatctttttg
     5281 ctaatcattt cacgattgta ggaatgggta aagactttac taataattta ggtttgagtt
     5341 acgaaaaatt aattaacatc gcattgttta ttactgcaac tattacagca ttggtagtgg
     5401 tgactgttgg aacattaccg ttcttaggac tagtaatacc aaatattatt tcaatttatc
     5461 gaggtgatca tttgaaaaat gctatccctc atacgatgat gttaggtgcc atctttgtat
     5521 tattttctga tatagttggc agaattgttg tttatccata tgaaataaat attggtttaa
     5581 caataggtgt atttggaaca atcattttcc ttatcttgct tatgaaaggt aggaaaaatt
     5641 atgcgcaaca ataataaaaa aataatgctt ttaattgcag taacgttatt aattagtatg
     5701 ctgtacttat ttgtaggtat tgattttgaa atatttgaat atcaattttc aagtcgttta
     5761 agaaagttca tattaattat tttagtaggt gctgccattg caacttcagt ggtgattttt
     5821 caagcgatta caaataaccg tctattgaca ccatcaataa tggggttaga tgcagtttat
     5881 ttatttatca aagtattgcc agtcttttta tttggaattc aatcggtatg ggttactaat
     5941 gtatatttga actttatatt aacacttata acgatggtgt tattcgcact aatcctattc
     6001 caaggtatct ttaaaattgg acatttttca atttatttta tcttacttat tggtgtcctt
     6061 ttaggaacat tttttagaag cataacaggt tttattcaac tgattatgga tcctgagtca
     6121 tttttagcaa tacaaagtag tatgtttgct aattttaatg cttctaattc gaatttagtt
     6181 attttctcag cagtgctatt agtaatctta ttagtcatta caattttact attgccttat
     6241 ttagatgtat tgcttttagg tcgtgctgaa gcaattaatc ttgggatatc gtatgaaaaa
     6301 ttaacgcgaa ttctacttgt aatagtctca gttttagttt ctgtgtcaac tgcattagta
     6361 ggaccaatta catttttagg tttattaact gtaaatctag cgcatgaact aatgaagacg
     6421 tatgaacata agtatatttt aattgcgaca atttgcttga gttggattag tttatttagt
     6481 gcgcaatggg tagttgaaaa tgtgtttgaa gctacgacag aaatgagtat acttattgat
     6541 ttgattggtg gaagttattt catttatcta ttagttagaa ggagaaatgc gcaatgattc
     6601 aagttgaaaa tttaactaaa actataaata atcaaatgat attggaagat attagcatag
     6661 atatcgaaaa aggtaaattg acttctttaa ttggacctaa tggtgcgggt aagagtactt
     6721 tactttcagc gatatgtagg ttaattcgtt ttgataacgg tgaagtgaaa atagatggac
     6781 agctcatgtc tgattataaa aataatgact tgtcgaaaaa aatatctata ttaaaacaaa
     6841 caaaccatac tgaaatgaat attacggtag agcagttggt aaactttgga cgattccctt
     6901 attctaaagg tcgtttgacg aaagaggatc atgatattgt caatgatgcg ctagatttgt
     6961 tgcaactaca agatatcaga aatcgtaata ttaagtcatt atctggtgga caacgtcagc
     7021 gtgcatacat tgcaatgaca atagcacaag atactgaata tattttgcta gatgaaccat
     7081 taaataattt agatatgaag catgctgttc aaattatgca aacgttaaaa atgttagcgc
     7141 ataaaatgaa taaagcgatt gtcattgtgt tacatgatat taactttgcg tcctgttatt
     7201 cagatcagat tgtagcattg aaaaacggac aactagttaa gtcagatttg aaagataatg
     7261 tcattcaaag tagtgtttta agtgatttat atgacatgaa tattcaaatt gaacatataa
     7321 gaaatcaaag gatttgttta tattttaagg attgataatt tggagacact ttaaaggggt
     7381 gatgcgccaa ttaaagaagg gttaaacgta aagcatttat ttatatttca catcaagcac
     7441 acagattaag ccaaaagagg agaatattat attatgaaga aaacagtctt atatttagta
     7501 ttagcagtaa tgtttttatt agcggcatgc ggtaacaatt ctgataaaga acaatcaaaa
     7561 tcagaaacta aaggttctaa agatacagta aaaattgaaa ataactataa aatgcgtggc
     7621 gagaaaaaag atggtagtga cgctaaaaaa gttaaagaaa ctgttgaagt accaaaaaat
     7681 cctaaaaatg cagttgtgtt agactatggc gcattagatg taatgaaaga aatgggctta
     7741 tcagataaag taaaagcatt acctaaaggg gaaggcggta agtcattacc gaatttctta
     7801 gaatcattta aagatgataa atatacaaac gttggtaatt taaaagaagt gaattttgat
     7861 aaaattgctg cgacgaaacc cgaagtaatc tttatctctg gacgtacagc taatcaaaag
     7921 aatttagatg aattcaaaaa agctgcacct aaagcgaaaa ttgtttatgt tggtgcagat
     7981 gaaaagaact taattggttc aatgaaacaa aacactgaaa atatcggtaa aatttacgat
     8041 aaagaagata aagctaaaga attaaataaa gatttagata acaaaattgc ttcaatgaaa
     8101 gataaaacga aaaacttcaa taaaactgtt atgtatttac tagttaacga aggtgaatta
     8161 tcaacatttg gacctaaagg tcgttttggt ggattagttt acgatacatt aggattcaat
     8221 gcagttgata aaaaagtaag taatagcaat catggacaaa atgtttctaa cgaatatgtt
     8281 aataaagaaa atccagatgt tattttagcg atggatagag gtcaagcgat aagtggtaaa
     8341 tcaactgcga aacaagcatt aaataatcct gtattaaaaa atgttaaagc aattaaagaa
     8401 gacaaagtat ataatttaga tcctaaatta tggtactttg cagctggatc aactacaact
     8461 acaattaaac aaattgagga acttgataaa gttgtaaaat aattttaaaa gaggggaaca
     8521 atggttaaag gtcttaatca ttgctcccct cttttcttta aaaaaggaaa tctgggacgt
     8581 caatcaatgt cctagactct aaaatgttct gttgtcagtc gttggttgaa tgaacatgta
     8641 cttgtaacaa gttcatttca ataccagtgg gctccaaaca tagagaaatt tgattttcaa
     8701 tttctactga caatgcaagt tggcggggcc caaacataga gaatttcaaa aaggaattct
     8761 acagaagtgg tgctttatca tgtctgaccc actccctata atgttttgac tatgttgttt
     8821 aaatttcaaa ataaatatga tagtgatatt tacagcgatt gttaaaccga gattggcaat
     8881 ttggacaacg ctctaccatc atatattcat tgattgttaa ttcgtgtttg catacaccgc
     8941 ataagattgc tttttcgtta aatgaaggct cagaccaacg cttaatggcg tgcttttcaa
     9001 actcattatg gcacttatag catggatagt atttattaca acatttaaat ttaatagcaa
     9061 taatatcttc ttcggtaaaa taatggcgac agcgtgtttc agtatcgatt aatgaaccat
     9121 aaactttagg catagacaaa gctccttaac ttacgattcc tttggatgtt caccaataat
     9181 gcgaacttca cgatttaatt caatgccaaa tttttctttg acggtctttt gtacataatg
     9241 aataaggttt tcataatctg tagcagttcc attgtctaca tttaccataa aaccagcgtg
     9301 tttagttgaa acttcaacgc cgccaatacg gtgaccttgc aaattagaat cttgtatcaa
     9361 tttacctgca aaatgaccag gcggtctttg gaatacacta ccacatgaag gatactctaa
     9421 aggttgttta gattctctac gttctgttaa atcatccatt ttagcttgta tttcagtcgt
     9481 tttaccagga gctaaagtaa atgcagcttc taatacaact aagtgttctt tttgaataat
     9541 gctattacga taatctaact ctaattcttt tgttgtaagt ttaattaacg agccttgttc
     9601 gtttacgcaa agcgcatagt ctatacaatc tttaacttcg ccaccataag cgccagcatt
     9661 catatacact gcaccaccga ttgaacctgg aataccacat gcaaattcaa ggccagtaag
     9721 tgcgtaatca cgagcaacac gtgagacatc aataattgca gcgccgctac cggctattat
     9781 cgcatcatca gatacttcga tatgatctag tgataataaa ctaattacaa tgccacgaat
     9841 accaccttca cggataataa tatttgagcc atttcctaaa tatgtaacag gaatctcatt
     9901 ttgataggca tatttaacaa ctgcttgtac ttcttcattt ttagtagggg taatataaaa
     9961 gtcggcatta ccacctgttt tagtataagt gtatcgtttt aaaggttcat caactttaat
    10021 tttttcattt gggataagtt gttgtaaagc ttgatagatg tctttattta tcacttctca
    10081 gtacatcctt tctcatgtct ttaatatcat atagtattat accaatttta aaattcattt
    10141 gcgaaaattg aaaagaaagt attagaatta gtataattat aaaatacgga attattgtcg
    10201 ttataagtat tttttacata gtttttcaaa gtattgttgc ttttgcatct catattgtct
    10261 aattgttaag ctatgttgca atatttggtg cttttttgta tggaattgca aagcaatatc
    10321 atcattagtt gataagaggt aatcaagtgc aagataagat tcaaatgttt gggtattcat
    10381 ttgaatgata tgtagacgca cctgttgttt tagttcatga aaattgttaa acttcgccat
    10441 cataactttc ttattatatt tatgatgcaa gcgataaaac cctacataat ttaagcgttt
    10501 ttcatctaag gatgtaatat catgcaaatt ttctacacct actaaaatat ctaaaattgg
    10561 ctctgttgaa tatttaaaat gatgcgtacc gccaatatgt tttgtatatt ttactgggct
    10621 gtctaagagg ttgaataata atgattcaat ttcagtgtat tgtgattgaa aacaattagt
    10681 taaatcacta ttaatgaatg gttgaacatt tgaatacatg ataaactcct ttgatattga
    10741 aatttaattt aatcacgata aagtctggaa tactataaca taattcattt tcataataaa
    10801 catgtttttg tataatgaat ctgttaagga gtgcaatcat gaaaaaaatt gttattatcg
    10861 ctgttttagc gattttattt gtagtaataa gtgcttgtgg taataaagaa aaagaggcac
    10921 aacatcaatt tactaagcaa tttaaagatg ttgagcaaaa acaaaaagaa ttacaacatg
    10981 tcatggataa tatacatttg aaagaaattg atcatctaag taaaactgat acaactgata
    11041 aaaatagtaa agaatttaag gcactacaag aagatgttaa aaaccatctc atacctaaat
    11101 ttgaagcata ttataagtca gcaaaaaatt tgcctgatga tacaatgaaa gttaagaaat
    11161 taaaaaaaga atatatgacg cttgcaaatg agaagaagga tgcgatatat caattaaaaa
    11221 aattcatagg tttatgtaat caatctatca agtataacga agacatttta gattatacga
    11281 aacaatttga aaaaaataga tacaaagttg aatcagaaat taaattagct gataataaaa
    11341 gtgaagcaac taatcttacg acaaaattag aacataataa taaagcgtta agagatactg
    11401 cgaagaagaa cctagatgat agtaaagaaa atgaagtaaa aggcgcgatt aaaaatcaca
    11461 ttatgccaat gattgaaaag caaattaccg atattaacca aactaatatt agtgataagc
    11521 atgttaataa tgcaaggaaa aacgcaatag aaatgtatta cagtctgcag aactattata
    11581 atacacgtat tgaaacaata aaggttagtg agaagttatc aaaagtcgat gtagataagt
    11641 tgccgaaaaa gggtatagat ataactcacg gcgataaagc ctttgaaaaa aagcttgaaa
    11701 aattagaaga aaaataacta taatcatttt tcaaagttaa aaattttgaa tttatggtta
    11761 acatgtcaac ttactatgtg tataatggta aacattgata ttaactatat gtataaaaat
    11821 gtcacgcaga tgctatttaa atgtgataaa tatttttaga ggtgaataga gtggctataa
    11881 agctaagttc aattgaccaa tttgaacagg ttattgagga aaataaatat gtttttgtat
    11941 taaaacatag tgaaacttgt ccaatatcgg caaatgcgta cgatcaattt aataaatttt
    12001 tatatgaacg cgatatggac ggttattatt tgattgtcca acaagaacgc gatttgtcag
    12061 attatattgc taaaaaaacg aacgttaaac atgaatcacc tcaagcattt tattttgtaa
    12121 atggtgaaat ggtttggaat cgagaccacg gtgatatcaa tgtgtcgtca ttagcacaag
    12181 cagaagaata atgaaactat agggttggaa cattttgcct tacactacta gacgtgaata
    12241 gcacaactta aattcgtgtg aatcagagta gtttggctat aatgatgttc tgacctttta
    12301 ttttatgtca cctttagaag cagttaagtt agtacttttt tacaaacata tgtataatgt
    12361 attcgagtat ttttattgaa aatattttgg aaaacgacga atccaataag aaaatttaaa
    12421 catgatttgt aagttagttt aataggaaat atatgctaaa ccaaaagaag catattgtta
    12481 tttactggaa taattaataa tcatgtcatg ttaaatgttc gcatataatc acgagataaa
    12541 atctaaaatt taagattaat cttttatgaa taaaaaacgt atcacaacaa ataataaagt
    12601 aaggtggtca aggttatgaa agtattagta gccatggatg agtttcatgg aattatttcg
    12661 agttatcaag ctaatagata tgttgaagag gcagttgcaa gccaaattga aactgcagat
    12721 gtagttcaag taccattgtt taatggaaga catgaattat tagattctgt atttttatgg
    12781 caatctgggc aaaagtatcg tataccagta catgatgcag atatgaatga agttgaaggt
    12841 gtttacggac aaactgatac agggatgacc gttatcgagg ggaatttatt tttaaaaggt
    12901 aaaaaaccaa ttgttgaacg aacaagttat ggtttaggag aaatgattaa acatgcatta
    12961 gataacgacg caaaacatgt tgtaatttca ctaggtggga ttgatagttt tgatgctggt
    13021 gcaggtatgt tacaagcatt aggtgctcaa ttctatgatg acgaagggcg tgtcgtagat
    13081 atgagacaag gtgctggtgt aattaaatat attcgtcgta tggatatgtc gaacttacac
    13141 cctaaaatgg aaacagcaag aattcaagta atgtcggatt tttcaagtcg attatatggt
    13201 aagcaaagtg aaatcatgca aacttatgat gcgcatcagt tgaatcataa tcaagcagca
    13261 gaaatcgata atttaatttg gtattttagt gagttattta aaagtgaatt gaaaattgca
    13321 attggtccag ttgaacgtgg tggtgctggt ggtggaattg cagcagtctt gaatggactg
    13381 tatcaagctg aaatattaac cagtcatgca ttagtagacc aactaacaca tttagaaaat
    13441 ttagttgaac aagcggattt aattattttt ggagaagggt taaatgaaaa tgatcagttg
    13501 ctagaaacga caacattgcg tattgcagaa ctttgtcata aacatcaaaa ggttgccatt
    13561 gcaatttgtg caactgctga aaagtttgat ttatttgaat cacaaggggt tacagcaatg
    13621 tttaatacat ttatcgatat gccagaaact tatactgact ttaaaatggg gttacaaatt
    13681 aggcattata cggttcagtc tttaaaactg ttgaaaacac attttaatgt tgaggtttag
    13741 taaagaagga ctaaattggt gatgctgtca tgttggttaa taacatttat gatggttagc
    13801 aaaacgaatt agaagatcga aagtatacgt aaaaaatatg aaaaatcacg ctatcattgc
    13861 actgaatgtt agcgtgattt ttttattaat taagcctgag ttggtatata atcgttggtt
    13921 tttagtgatt ttcagcgata tcttctacaa ttccaatgat tacttgtact gctttttcca
    13981 taacatcaat ggatgcatat tcatatgggc cgtggaagtt accgcaacct gtaaagatgt
    14041 ttggagttgg taaccccata aatgacaatt gtgaaccatc tgtaccaccg cgaataggtt
    14101 cagtgtttgc tggaatatct aatttggcaa agacacgttt aggtatatca ataatatgag
    14161 gcaatggtaa tattttttct gccatattga aatattgatc cgatatatca actttaactg
    14221 gataattttc aaaatgggca ttgatatcgt cacgtatttc taaaatacgt ttcttacgca
    14281 attcgaattg ttttttatca tgatcacgaa taatgtattg caaagttgct ttttcaacag
    14341 ttccttcaaa gttcattaag tgataaaagc cttcgtatcc ttctgttcgc tccggaactt
    14401 cactatcagg tagcaaacta tcgaattgtt cacctaaacg tattgcgttt accattgcat
    14461 ttttagctga accaggatga acatttacac cgtggcatgt aataaccgct tcagcagcgt
    14521 taaagctttc atattgtaat tctccatatt gactaccatc catagtataa gcaaaatcag
    14581 cattgaagcg gtcaacatca aatttatgtg gaccacgacc gatttcttcg tctggtgtaa
    14641 atccaatgcg aatggtacca tgtttaattt ctggatgttc ttgtaaataa caaatagctt
    14701 ccataatttc cacaataccc gctttatcgt ctgcacctag taacgatgta ccatcagtta
    14761 ccattaatgt atgaccaact aaactgttaa gttctggaaa tactttagga tctaagacac
    14821 gtttagtatt gcctagtttg tatggcttac catcatagtt ttcaataatt tgcggtttaa
    14881 catttgaagc attgaaatca ggtgatgtat caacatgcgc caaaaatcca actgttggga
    14941 catcgacatc gatgttactt tctaatgtag caaataagta gccattttca tctaaatcag
    15001 ttggcaatcc taattgttgt aattcttttt ctaataaatg taacaaatcc cattgctttt
    15061 cagttgaagg tgttgttgta gattttggat cagattgcgt atcaattgtc gtatatcttg
    15121 ttaatctatc tatcaattgg ttcttcatta tattcgaccc cttaaactct attattcatg
    15181 ttgtaagatt ttttatatgt cttacctttg attttaccat acagttgttt gatacgtgtg
    15241 tataggtaat atagaatttc agaaactaat ataccgaaag caatcgcacc tgaaatcagt
    15301 gtaacttcta aaaatgtatt tacagcactt gtataatcat ttgatactaa aaaacgagtc
    15361 gcttgataag ctgcaccacc aggtactaat ggtataatgc ctggcactat gaatataatt
    15421 accggtcgtt tatatctgcg gctcatagta tgactcatta agcctaaaat taagcttccc
    15481 aaaaatgaag cgccaacttt tccaaactct aaatctaccg ttaattggta aatcgtccat
    15541 gcaatggcac ccacaaatcc acatgctact aagaggcgtt tgggtgcatt gaaaatgata
    15601 gagaaaagta ctgttgatat aaagctgatt gtaaaatgaa ataaataaaa tagcatgctt
    15661 taacagtcct tccttaaatg attaataaaa cgattgcgac accagcaccg attgcgaatg
    15721 ctgttaatgc agcttcaaca ccacgagaca tacctgcaag taattcaccc gctaataaat
    15781 ctcgaatggc attggtaatt aatataccag ggacaagtgg catgacactg gctatagtaa
    15841 tgatatcttg attggttgca atgcctaatt tagtaaatgt ggctgcaatg gatatgacca
    15901 cagcggctgc aacaaactct gagaaaaatt taatttgtat atagcgttgc acaaagctga
    15961 atgttaaaaa tgcggatccg ccagcaatga ctgcaatcca acaatctgat gcgacaccac
    16021 caaacataaa taggaagaag ccacatgcaa tggcagctgc aaagaaattc gttaaaaaag
    16081 aatattgtaa tgatgcatgc tgtaaatgaa taaattcaga tttagcttca tcaattgtga
    16141 gttctttatt tgatatttta cgtgaaagac tattcgttaa agcgattttc tctaaatctg
    16201 ttgtacgctc ttgtacacga attaatcttg tacttgttcg atcgtttaat gaaaaaataa
    16261 ttgcagttga actgacaaaa ctatatgtat tatgaagacc ataactatgt gcgatacggt
    16321 tcattgtatc ttcaactcga tatgtttcag cacctgattc aagtaaaatt ctacctgcaa
    16381 ttaatacaac atcaatcact ttgttttcat ctataattgt gattgaatct ggcatatcaa
    16441 ttcacctcca atgatatgtg ttatttattt gaacaattga agtttacaac ttgttgttgc
    16501 aactttcaat agtgagactt tgtgttagta tgatgaactt gtatggttca aatttaaata
    16561 agaaaaactg ttaatctttg ctattatact atgatttaat aatagcaaag gattaacagt
    16621 tttgtcgttg ttataaattg ataatagggt taaacattac tttgtttcgc ccttgatttt
    16681 tggctacatg caccatatcg tctgcatctt taaacacttt acgctgtgat tttggatcgt
    16741 catctgttaa atatccaaca ccgatagaca ctgacaattt aataacttct ttgtttggta
    16801 aatggaatga tgatttttca acacccgaac gaatattttc agccaattta acactttgat
    16861 caagtgaata attgtgaatg acaactgaga actcttcgcc accatttcta aaaattttaa
    16921 attgattcgg cacatagttt ttaagtaatt gagacatttg ttttaataca gcatcacctg
    16981 atttgtgtga gtaggtatca ttgacatctt taaatccatc gatatcgatt aataataaag
    17041 cgatactttg atgttctttt tcagcttttc gtgaaatttc atttaaatgt ctatcaaatt
    17101 cttttacatt acctaagcct gttaagtaat catatttatc ttcgttttca taacgattta
    17161 cgagtgagaa gaaatgccaa atatcgacaa atgttatcgc tgaagctaaa gtgataatta
    17221 atgaaattgg tattaaaatg ataacttccg atagtgtgta aataggactc actaacgcga
    17281 caccaaataa aatgattatt gtaacaacat taagtattaa taatgatagc acatcatttt
    17341 gttttaaaaa tggtccaata gcacttgtta ctgcagcaat aacaatcaac gtaacaccgt
    17401 acataatcga gttgttaaat actacaattt caacaattgc tacaattact gtggcagata
    17461 atgtatagac catatttgta aatctaccta aaaacaataa aggaacgaat gttaagtgaa
    17521 ttaaataatc ttcacgataa gggatagggt agacagataa taataatgat acgattgtca
    17581 ttaaaacagt gacataagcc ttagaaaaaa ccatacgttt gttttctgaa tactgtaagc
    17641 gatggaataa atagattcca gcgactataa cagatatatt gtatataaat gcttcgaaca
    17701 tgtctgaatc gactccttta attgaccact agctattgta agtgaaaact tacaatttgt
    17761 cattagttta catataaaat taatgtatga tatagacttt gatgttaaaa tgttgcctta
    17821 aatgatatga tgaaaaaatg aataataggc gatatataag aaatgaatcg tatagttgta
    17881 aatatgatat cattgattga gcgaattaat ttata
//
LOCUS       DAMZLE010000043        17688 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMZLE010000043 DAMZLE010000000
VERSION     DAMZLE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.043"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            135..1007
                     /gene="dprA"
                     /locus_tag="POB92_001295"
     CDS             135..1007
                     /gene="dprA"
                     /locus_tag="POB92_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI6578803.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1181..3256
                     /gene="topA"
                     /locus_tag="POB92_001296"
     CDS             1181..3256
                     /gene="topA"
                     /locus_tag="POB92_001296"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI6578804.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3412..4719
                     /gene="trmFO"
                     /locus_tag="POB92_001297"
     CDS             3412..4719
                     /gene="trmFO"
                     /locus_tag="POB92_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDI6578805.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            5136..6032
                     /gene="xerC"
                     /locus_tag="POB92_001298"
     CDS             5136..6032
                     /gene="xerC"
                     /locus_tag="POB92_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI6578806.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     KTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            6029..6574
                     /gene="hslV"
                     /locus_tag="POB92_001299"
     CDS             6029..6574
                     /gene="hslV"
                     /locus_tag="POB92_001299"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDI6578807.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            6640..8043
                     /gene="hslU"
                     /locus_tag="POB92_001300"
     CDS             6640..8043
                     /gene="hslU"
                     /locus_tag="POB92_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDI6578808.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            8068..8841
                     /gene="codY"
                     /locus_tag="POB92_001301"
     CDS             8068..8841
                     /gene="codY"
                     /locus_tag="POB92_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDI6578809.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISGVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            8892..8984
                     /locus_tag="POB92_001302"
     CDS             8892..8984
                     /locus_tag="POB92_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578810.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            9183..9950
                     /gene="rpsB"
                     /locus_tag="POB92_001303"
     CDS             9183..9950
                     /gene="rpsB"
                     /locus_tag="POB92_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI6578811.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            9984..10097
                     /locus_tag="POB92_001304"
     CDS             9984..10097
                     /locus_tag="POB92_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578812.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            10132..11013
                     /locus_tag="POB92_001305"
     CDS             10132..11013
                     /locus_tag="POB92_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI6578813.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            11150..11872
                     /locus_tag="POB92_001306"
     CDS             11150..11872
                     /locus_tag="POB92_001306"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI6578814.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            11891..12445
                     /gene="frr"
                     /locus_tag="POB92_001307"
     CDS             11891..12445
                     /gene="frr"
                     /locus_tag="POB92_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI6578815.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            12818..13588
                     /locus_tag="POB92_001308"
     CDS             12818..13588
                     /locus_tag="POB92_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDI6578816.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            13595..14377
                     /locus_tag="POB92_001309"
     CDS             13595..14377
                     /locus_tag="POB92_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6578817.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            14589..15875
                     /gene="rseP"
                     /locus_tag="POB92_001310"
     CDS             14589..15875
                     /gene="rseP"
                     /locus_tag="POB92_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDI6578818.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASERTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            15895..17598
                     /locus_tag="POB92_001311"
     CDS             15895..17598
                     /locus_tag="POB92_001311"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI6578819.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKYSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
BASE COUNT         6543 a         2453 c         3420 g         5272 t
ORIGIN      
        1 ccaacatact tttcactttt taagtcagta tttttactcc ttaaaataac attacatcga
       61 caatgttttt gtattagaaa tgtaaaacaa aaatagaata tgattttgta tgattacaaa
      121 aaggagttga tttattgatt aaactatttt tgcttaagtt atactgggca cacttttcga
      181 ctaaacaaat tcatcaattt ttaatggcat atcctaatgt aattaaagag gagggaagaa
      241 aaaaagatag ttatttatgt gaatgggtga atagggaaga aaatgttcat ttattacgta
      301 aatactatgc ttttataaaa cttgatcata acgatattat taaagaactg cagaaattaa
      361 aagtaagtta cattacatat atggattctg aatacccagt gctattaaaa gaaatatatc
      421 aatttccatt acttcttttc tataaaggga acatcaaatt aataaataat atgcatcatt
      481 tggcagtagt aggtgcaaga gattctacaa gttataccca acagtcttta gaatttttat
      541 tatcaaatga taaaagcaaa tatttaacaa ttgtttccgg ccttgctcaa ggagctgatg
      601 caatggcaca tcaaatagct ttaaaataca atctccctac aattgcagtt ttagcctttg
      661 gccatcaaac acattatccc aaaagtacat tagcattaag aaataaaata gaagaaaaag
      721 gtttagttat atccgaatat ccaccacata caccaattgc taaatataga tttcctgagc
      781 gcaatagaat tatcagcggt ttgtcaaaag gggttttaat tactgaggct aaggaacaaa
      841 gtggcagtca catcacgata gattttgcat tagagcaaaa tagaaatgtt tatgttttac
      901 ctggatctat gtttaatcct atgacaaaag gtaatttatt acgtatccaa gaaggtgcta
      961 aggtagtatt aaacgctaat gatatatttg aagactacta tatttaaaac taaaaataat
     1021 gagttgcttc atatttcttt tgttaccctt tgtaatttat tttttaataa aatctataaa
     1081 aaatagacag ggaaaatgat ttgtttagat ataaaacgtt gacaaaagca aaattaagcg
     1141 tttatcattt atctttagta attagattag cgagggggaa atgacattgg cagataattt
     1201 agtcattgtt gaatcgcctg caaaagcaaa aaccattgaa aagtatttag gtaagaaata
     1261 taaagttata gcttcaatgg gacacgtcag agacttacca agaagtcaaa tgggtgtcga
     1321 cactgaagat aattacgaac caaaatatat aacaatacgc ggaaaaggtc ctgttgtaaa
     1381 agaattgaaa aaacatgcaa aaaaagcgaa aaacgtcttt ctcgcaagtg accccgaccg
     1441 tgaaggtgaa gcaattgctt ggcatttatc aaaaatttta gagcttgaag attctaaaga
     1501 aaatcgcgtt gttttcaacg aaataactaa agacgctgtt aaagaaagtt ttaaaaatcc
     1561 tagagaaatt gaaatgaact tagtcgatgc acaacaagcg cgtcgaatat tagatagatt
     1621 ggttggctat aacatctcgc cagttctatg gaaaaaagta aaaaaaggtt tgtcagcggg
     1681 tcgagttcaa tctgttgcac ttcgtttagt cattgaccgt gaaaatgaaa ttcgaaactt
     1741 taaaccagaa gaatattgga ctattgaagg agaatttaga tacaaaaaat caaaattcaa
     1801 tgctaaattc cttcattata aaaataaacc ttttaaatta aaaacgaaaa aagatgttga
     1861 gaaaattaca gctgcactag atggagatca attcgaaatt acaaacgtga ctaaaaaaga
     1921 aaaaacgcgt aatccagcaa acccatttac aacttctaca ttacaacaag aggcggcacg
     1981 taaattaaac tttaaagcaa gaaaaacaat gatggtcgca caacaattat atgaaggtat
     2041 agatttgaaa aaacaaggta cgattggttt aataacatat atgagaaccg attctacacg
     2101 tatttcagat actgccaaag ctgaagcaaa acagtatata actgataaat acggtgaatc
     2161 ttacacttct aaacgtaaag catcagggaa acaaggtgac caagatgccc atgaggctat
     2221 tagaccttca agtactatgc gtacgccaga tgatatgaag tcatttttga cgaaagacca
     2281 ataccgatta tacaaattaa tttgggaacg atttgttgct agtcaaatgg ctccagcaat
     2341 acttgataca gtctcattag acataacaca aggtgacatt aaatttagag cgaatggtca
     2401 aacaatcaag tttaaaggat ttatgacact ttatgtagaa actaaagatg atagtgatag
     2461 cgaaaaggaa aataaactgc ctaaattaga gcaaggtgat aaagtcacag caactcaaat
     2521 tgaaccagct caacactata cacaaccacc tccaagatat actgaggcga gattagtaaa
     2581 aacactagaa gaattgaaaa ttgggcgacc atcaacttat gcaccgacaa tagatacgat
     2641 tcaaaagcgt aactatgtca aattagaaag taagcgtttt gttcctactg agttgggaga
     2701 aatagttcat gaacaagtga aagaatactt cccagagatt attgatgtgg aattcacagt
     2761 gaatatggaa acgttacttg ataagattgc agaaggcgac attacatgga gaaaagtaat
     2821 cgacggtttc tttagtagct ttaaacaaga tgttgaacgt gctgaagaag agatggaaaa
     2881 gattgaaatc aaagatgagc cagccggtga agactgtgaa gtttgtggtt ctcctatggt
     2941 tataaaaatg ggacgctatg gtaagttcat ggcttgctca aacttcccgg attgtcgtaa
     3001 tacaaaagcg atagttaagt ctattggtgt taaatgtcca aaatgtaatg atggtgacgt
     3061 cgtagaaaga aaatctaaaa agaatcgtgt cttttatgga tgttcgaaat atcctgaatg
     3121 cgactttatc tcttgggata agccgattgg aagagattgt ccaaaatgta accaatatct
     3181 tgttgaaaat aaaaaaggca agacaacaca agtaatatgt tcaaattgcg attataaaga
     3241 ggcagcgcag aaataatatt tttatttcct agatacattt taaggttgtt aaatagaatc
     3301 attagtgaat cttattttaa agatagtaat ggattaatct aaataagtgc ggataatata
     3361 aacataacaa cataattaaa agacataaat gacaataaaa ggagtataga aatgactcaa
     3421 actgtaaatg taataggtgc tggtcttgcc ggttcagaag cggcatatca attagctgaa
     3481 agaggaatta aagttaatct aatagagatg agacctgtta aacaaacacc agcgcaccat
     3541 actgataaat ttgcggaact tgtatgttcc aattcattac gaggaaatgc tttaactaat
     3601 ggtgtgggtg ttttaaaaga agaaatgaga agattgaatt ctataattat tgaagcggct
     3661 gataaggcac gagttccagc tggtggtgca ttagcagttg atagacacga tttttcaggt
     3721 tatattactg aaacacttaa aaatcatgaa aatatcacag ttattaatga agaaattaat
     3781 gccattccag atggatacac aattatcgca acaggaccac ttactacaga aacccttgcg
     3841 caagaaatag tggacattac tggtaaagat caactttatt tctatgatgc ggctgctcca
     3901 attattgaaa aagaatctat tgatatggat aaagtttact taaagtcccg ttatgataaa
     3961 ggtgaagctg catatttaaa ctgtcctatg actgaggatg aatttaatcg cttttatgat
     4021 gcagtattag aagctgaagt tgcgcctgta aattcatttg aaaaagaaaa atatttcgag
     4081 ggttgtatgc cttttgaagt aatggcagaa cgcggacgca agacattact atttggacca
     4141 atgaaaccag taggattaga agatccaaag actgggaaac gtccttatgc agtggttcaa
     4201 ttaagacaag atgatgctgc tggcacactc tacaatattg tcggcttcca aacgcattta
     4261 aaatggggag ctcaaaaaga agttattaaa ttaattccag gtttagaaaa tgttgatatt
     4321 gttagatatg gtgtgatgca tagaaatacc ttcattaact caccggacgt attaaacgag
     4381 aaatatgaat tgatttcaca accaaacata cagtttgcgg gacaaatgac tggtgttgaa
     4441 ggttatgtag aaagcgcagc tagcggctta gttgcaggta tcaatcttgc gcataaaata
     4501 ttaggcaaag gtgaggtagt atttcctaga gaaacgatga ttggaagtat ggcttactat
     4561 atttctcatg ctaaaaataa taagaatttc caacctatga atgctaactt cgggttatta
     4621 ccatctttag aaactagaat taaagataaa aaagaacgct atgaagcaca agctaataga
     4681 gctttggatt acttagaaaa tttcaaaaaa actttataaa atagttagaa agactagata
     4741 tgctattcat tcttaagtca tcaacgagta agtaatgact ttctaaatgg aaaatactta
     4801 tcctagtctt tttaattttg gaattgttac gtatttctga caatttagaa ttcgcattca
     4861 aaaaatatct aaataaataa cacgcaataa gttgattgat gtaacatgta agagaatgtt
     4921 ttaaataaac tttatttaaa aggcaatgaa ataataaatg gcaaggctat taataaagac
     4981 ttttagtaat taatttaaaa aagaggtatt ctaattaaca ggttttccga ttagttacaa
     5041 ttatttaatt ctcaaaagat ttagaattga ttatcaaatt actgtaagcc ctttgctgta
     5101 tatgctacaa ttcttattga tggagggtaa atgtattgaa tcatattcaa gaagcgtttt
     5161 taaatacatt gaaagttgaa cggaattttt cggaacatac attgaaatca tatcaagatg
     5221 acttaattca gtttaatcaa tttttagaac aagaacattt acagttgaaa acttttgaat
     5281 acagagatgc tagaaattat ttgagctatt tatattcaaa tcatttgaaa agaacatctg
     5341 tttctcgtaa aatctcaacg ttaagaactt tctatgaata ttggatgacg cttgatgaga
     5401 acattattaa tccatttgtt caattagtac atccgaaaaa agaaaaatat cttccgcaat
     5461 tcttttacga agaagaaatg gaagcgttat ttaaaactgt agaagaggac acttcaaaaa
     5521 gtttacggga tcgagttatt cttgaattgt tatatgctac aggtatccgt gtttcggaat
     5581 tagttaatat taaaaaacaa gatatagatt tttacgcgaa tggtgttacc gtattaggaa
     5641 aagggagcaa agagcgcttt gtaccgtttg gtgcttattg tagacaaagc atcgaaaatt
     5701 atttagaaca tttcaaacca attcagtcat gcaatcatga ttttcttatt gtaaatatga
     5761 agggtgaagc aatcactgaa cgcggtgtac gatatgtttt aaatgatatt gttaaacgaa
     5821 cagcaggcgt aagtgagatt catccccaca agctcagaca tacatttgca acgcatttat
     5881 tgaatcaagg tgcagaccta agaacagtac aatcgttatt aggtcatgtt aatttgtcaa
     5941 caactggtaa atatacacac gtatctaacc aacaattaag aaaagtgtat ctaaatgcac
     6001 atcctcgagc gaaaaaggag aatgaaacat gagtaataca acattacatg caacaacaat
     6061 ttatgctgta agacataatg ggaaagcagc tatggctgga gatgggcaag taacgcttgg
     6121 tcaacaagtc atcatgaaac aaacggcaag aaaagtgcga cgtttatatg aaggtaaagt
     6181 gttagctggt ttcgcaggta gtgtagcaga tgcgtttacg ttatttgaaa aattcgaaac
     6241 aaaattacaa cagtttagtg gtaacttaga aagagctgct gttgaattgg cacaagaatg
     6301 gcgaggcgat aaacaattac gtcaattaga agctatgcta attgtaatgg ataaagatgc
     6361 tattttagtt gtcagtggaa ctggcgaagt tattgcccca gatgatgacc ttatcgctat
     6421 tggatcagga ggcaactacg cattaagcgc aggacgtgca ttgaaacgcc atgcatcgca
     6481 tttgtctgct gaagaaatgg catatgagag cttgaaagta gcggctgata tttgtgtctt
     6541 taccaacgat aatattgttg ttgaaacact ataataatca gagcacgata aataattacg
     6601 agcaattaat tttagttaaa agacggagga atgaaattaa tggatacagc tggaataaga
     6661 ttaactccaa aagaaatcgt atctaaatta aatgaataca tcgttggaca aaatgatgct
     6721 aaacgtaaag tggcaattgc cttacgtaat cgatacagaa gaagtttatt agatgaggaa
     6781 tcaaagcaag aaatttcacc taaaaatatt ttgatgattg gaccaactgg cgttggtaaa
     6841 actgaaattg caagaagaat ggccaaagtt gtcggcgcgc catttataaa agtagaagct
     6901 actaaattta ctgaggtagg ttatgtagga cgagatgttg aaagtatggt tagagatctt
     6961 gttgatgttt cagtaagatt agtcaaggca cagaaaaaat cattggtaca agatgaagca
     7021 acagctaagg ccaatgaaaa acttgttaag ttattagttc caagtatgaa aaagaaagcg
     7081 tctcaaacga ataatccttt agagtcactt ttcggaggtg caattccaaa tttcggacaa
     7141 aataacgaag atgaagaaga accacctact gaggaaatta aaacaaaacg ttctgaaatt
     7201 aagagacagc tagaagaagg caaacttgaa aaagaaaagg taagaattaa agtcgaacaa
     7261 gatcctggtg ctttaggtat gctaggtaca aatcaaaatc agcaaatgca agagatgatg
     7321 aatcaattaa tgcctaaaaa gaaagttgag cgagaagttg ctgttgagac ggcaaggaaa
     7381 atcttagctg atagttatgc ggatgaacta attgatcaag aaagcgctaa ccaagaagcg
     7441 cttgaattag cagaacaaat gggtatcatc tttatagatg aaatcgacaa agttgcgacg
     7501 aataatcata atagtggtca agatgtctca agacaaggtg ttcaaagaga tattttacct
     7561 atacttgaag gtagcgttat tcaaaccaaa tatggtactg tgaatactga acatatgctg
     7621 tttataggtg ctggagcttt ccatgtatct aagccgagtg acttgatacc agaattgcaa
     7681 ggtcgttttc cgattagagt tgaacttgat agtttatcgg tagaagattt tgtaagaatt
     7741 ttgacagaac caaaattgtc attaattaaa caatatgaag cattgcttca aacagaagaa
     7801 gttactgtaa actttaccga tgaagcaatt actcgtttag ctgagattgc ttatcaagta
     7861 aatcaagata cagacaacat tggtgcacgt cgacttcata caattttaga aaagatgtta
     7921 gaagatttat cattcgaagc accaagtatg ccgaatgcag ttgtagatat taccccacaa
     7981 tatgttgatg ataaattaaa atcaatttca acaaataaag atttaagtgc atttattcta
     8041 taaaaatata caaaaggaga aaaattcatg agcttattat ctaaaacgag agagttaaac
     8101 acgttacttc aaaaacacaa aggtattgcg gttgatttta aagatgtagc acaaacgatt
     8161 agtggcgtaa ctgtaacaaa tgtatttatt gtatcgcgtc gaggtaaaat tttagggtcg
     8221 agtctaaatg aattattaaa aagtcaaaga attattcaaa tgttggaaga aagacatatc
     8281 ccaagtgaat atacagaacg attaatggaa gttaaacaaa cagaatcaaa tattgatatc
     8341 gacaatgtat taacagtttt tccacctgaa aacagagaat tattcataga tagtcgtaca
     8401 actatcttcc caattttagg tggaggagaa agattaggta cattagtact tggtcgagta
     8461 catgatgatt ttaatgaaaa tgatttggta ctaggtgaat atgctgctac agttattggt
     8521 atggaaatct tacgtgagaa gcatagtgaa gtagaaaaag aagcgcgcga taaagctgct
     8581 attacaatgg caattaattc attatcttat tctgaaaaag aagcgattga acatatcttt
     8641 gaagaacttg gcggtacgga aggcctatta atcgcatcaa aagttgcaga tagagttggt
     8701 attactagat ctgtaattgt aaatgcacta cgtaaattag aaagtgctgg tgtaattgaa
     8761 tcacgttctt taggaatgaa aggtactttc attaaagtta aaaaagaaaa attcttagat
     8821 gaattagaaa aaagtaaata agtcgatgag tctgggacat aattcaatgt cctagtcgtg
     8881 acgatgttat cttggcggta gtcaacgaaa tgactttgat gattatgttg aagtcatttc
     8941 tttatttttg tctaattttc aatcaaattg atattccaca ttaacatgat agaattacct
     9001 ttaaatttat gaaaaaattc ttgaaaaaca tttgctttag acacggcatt atgttatatt
     9061 tattttcggc tataaagcca atacacacat atcaaacgtg attgtgtaaa gggtgcaata
     9121 tttgtatatt gtctttacaa tagtttgcta tggaggtaat taaccaatag gaggaattta
     9181 taatggcagt aatttcaatg aaacaattac tagaagcggg tgttcacttc ggtcaccaaa
     9241 cacgtcgttg gaacccaaaa atgaaaaaat atatcttcac tgagagaaat ggtatttata
     9301 tcatcgactt acaaaaaaca gtgaaaaaag tagacgaggc atacaacttc ttgaaacaag
     9361 tttcagaaga tggtggacaa gtcttattcg taggaactaa aaaacaagca caagaatcag
     9421 ttaaatctga agcagaacgt gctggtcaat tctacattaa ccaaagatgg ttaggtggat
     9481 tattaactaa ctataaaacg atctcaaaac gaatcaaacg tatttctgaa attgaaaaaa
     9541 tggaagaaga tggtttattc gaagtattac ctaaaaaaga agtagtagaa cttaaaaaag
     9601 aatacgaccg tttaatcaaa ttcttaggcg gaattcgtga tatgaaatca atgcctcaag
     9661 cattattcgt agttgaccca cgtaaagagc gtaatgcaat tgctgaagct cgtaaattaa
     9721 atattcctat cgtaggtatc gttgacacta actgtgatcc tgacgaaatt gactacgtta
     9781 tcccagcaaa cgacgatgct atccgtgcgg ttaaattatt aactgctaaa atggcagatg
     9841 caatcttaga aggtcaacaa ggcgtttcta atgaagaagt agctgcagaa caaaacatcg
     9901 atttagatga aaaagaaaaa tcagaagaaa cagaagcaac tgaagaataa tcaactgttg
     9961 aatctgactt agatatagtt taaatgggtg ataagatatt aatgcttatc acctttttta
    10021 aaaagaaaat cgaggcaaat tacaaatatt caattagagt attagcaatc ttgcctataa
    10081 taatgctaaa atcataatat ataaaatgat aacttattgg aggaataatg aatggcaact
    10141 atttcagcaa aacttgttaa agaattacgt gaaaaaactg gcgcgggtat gatggattgt
    10201 aaaaaagcgc taactgaaac tgatggtgac atcgataaag cgattgatta cctacgtgaa
    10261 aaaggtattg ctaaagcagc taaaaaagca gaccgtattg cggctgaagg tttagtacat
    10321 gtagaaacta aaggtaacga cgcagttatc gttgaaatca actctgaaac agactttgtt
    10381 gctcgtaacg aaggattcca agagttagtt aaagaaatcg ctaatcaagt attagataca
    10441 aaagctgaaa ctgttgaagc tttaatggaa acaactttac caaatggtaa atcagttgat
    10501 gaaagaatta aagaagcaat ttcaacaatc ggtgaaaaat taagtgttcg tcgttttgct
    10561 atcagaacta aaactgataa cgatgctttc ggcgcttact tacacatggg tggacgcatt
    10621 ggtgtattaa cagttgttga aggttcaact gacgaagaag cagcaagaga cgttgctatg
    10681 catatcgctg caatcaaccc taaatatgtt tcttctgaac aagttagcga agaagaaatc
    10741 aaccacgaaa gagaagtttt aaaacaacaa gcattaaatg aaggtaaacc agaaaacatc
    10801 gttgaaaaaa tggtggaagg acgtttacgt aaatacttac aagaaatttg tgctgtagat
    10861 caagacttcg ttaaaaaccc tgatgtaaca gttgaagctt tcttaaaaac aaaaggtgga
    10921 aaacttgttg acttcgtacg ctatgaagta ggcgaaggta tggaaaaacg cgaagaaaac
    10981 tttgcggatg aagttaaagg acaaatgaaa taatctgtca taaagtaaaa caaggaagaa
    11041 gacaccttta atgttgcttt attaaaatgt aaatcattct aataaaacga caactgtgtc
    11101 ttctttactt gtatatgtta catatattca cgatagagag gataagaaaa tggctcaaat
    11161 ttctaaatat aaacgtgtag ttttgaaact aagtggtgaa gcgttagctg gagaaaaagg
    11221 atttggcata aatccagtaa ttattaaaag tgttgctgag caagtggctg aagttgctaa
    11281 aatggactgt gaaatcgcag taatcgttgg tggcggaaac atttggagag gtaaaacagg
    11341 tagtgactta ggtatggacc gtggaactgc tgattacatg ggtatgcttg caactgtaat
    11401 gaatgcctta gcattacaag atagtttaga acaattggat tgtgatacac gagtattaac
    11461 atctattgaa atgaagcaag tggctgaacc ttatattcgt cgtcgtgcaa ttagacactt
    11521 agaaaagaaa cgcgtagtta tttttgctgc aggtattgga aacccatact tctctacaga
    11581 tactacagcg gcattacgtg ctgcagaagt tgaagcagat gttattttaa tgggcaaaaa
    11641 taatgtagat ggtgtatatt ctgcagatcc taaagtaaac aaagatgcgg taaaatatga
    11701 acatttaacg catattcaaa tgcttcaaga aggtttacaa gtaatggatt caacagcatc
    11761 ctcattctgt atggataata acattccgtt aactgttttc tctattatgg aagaaggaaa
    11821 tattaaacgt gctgttatgg gtgaaaagat aggtacgtta attacaaaat aaatttagag
    11881 gtgtaaaata atgagtgaca ttattaatga aactaaatca agaatgcaaa aatcaatcga
    11941 aagcttatca cgtgaattag ctaatatcag tgcaggaaga gctaattcaa atttattaaa
    12001 cggcgtaaca gttgattact atggtgcacc aacacctgta caacaattag caagcatcaa
    12061 tgttccagaa gcacgtttac ttgttatttc tccatacgac aaaacttctg tagctgacat
    12121 cgaaaaagcg ataatagcgg ctaacttagg tgtcaaccca acaagtgatg gtgaagtgat
    12181 acgtattgca gtacctgcct taacagaaga gcgtagaaaa gagcgcgtta aagatgttaa
    12241 gaaaattggt gaagaagcta aagtatctgt tcgaaatatt cgtcgtgata tgaatgatca
    12301 gttgaaaaaa gatgaaaaaa atggcgacat tactgaagat gagttgagaa gtggtactga
    12361 agatgttcag aaagcaacag acaattcaat aaaagaaatt gatcaaatga ttgctgataa
    12421 agaaaaagat attatgtcag tataaaacta atatacaatg acatattaaa atgccagtat
    12481 taaacgataa tgtaacattt aaaatgggca tgtttaatta aatcaaagat gcatgtgatg
    12541 atttaaattc acaatgagca taaaaatggt gtttaaacaa gttaattaaa catatacttt
    12601 ataaataata ggcattaggt atattgctat aataaagtta tgtaattttt aacctcagta
    12661 tgtatgtcac atttctggtg taaactgtac cgagtcagac tttggtacag tttttttatt
    12721 tgcttattca atgcattaaa tgagtatgat aaaatgataa tgattgttta gtaacttata
    12781 ctatatgaca gagatgatca ggctcggagg aaagaccatg tttaaaaagc taataaataa
    12841 aaagaacact ataaataatt ataatgaaga attagactcg tctaatatac ctgaacatat
    12901 cgctattatt atggatggta atgggcgatg ggctaagaag cgaaaaatgc ctagaattaa
    12961 aggtcattac gaaggtatgc aaacaataaa aaaaattact agggtagcta gtgatattgg
    13021 tgttaagtac ttaactttat acgccttttc cactgaaaat tggtcaagac ctgaaagtga
    13081 agtaaattat attatgaatt tgcctgtcaa tttcttaaag acattcttac cggaactaat
    13141 tgaaaaaaat gtcaaagttg aaacaattgg atttactgat aagttgccaa aatcaacgat
    13201 agaagcaatt aataatgcta aagaaaagac agctaataat accggcttaa aattaatatt
    13261 tgcaattaat tatggtggca gagcagaact tgttcatagt attaaaaata tgtttgacga
    13321 gcttcatcaa caaggtttaa atagtgatat catagatgaa acatatataa acaatcattt
    13381 aatgacaaaa gactatcctg atccagagtt gttaattcgt acttcaggag aacaaagaat
    13441 aagtaatttc ttgatttggc aagtttcgta tagtgaattt atctttaatc aaaaattatg
    13501 gcctgacttt gacgaagatg aattaattaa atgtataaaa atttatcagt cacgtcaaag
    13561 acgctttggc ggattgagtg aggagtagta tagtatgaaa gttagaacgc tgacagctat
    13621 tattgcctta atcgtattct tgcctatctt gttaaaaggc ggccttgtgt taatgatatt
    13681 tgctaatata ttagcattga ttgcattaaa agaattgttg aatatgaata tgattaaatt
    13741 tgtttcagtt cctggtttaa ttagtgcagt tggtcttatc atcattatgt tgccacaaca
    13801 tgcagggcca tgggtacaag taattcaatt aaaaagttta attgcaatga gctttattgt
    13861 attaagttat actgtcttat ctaaaaacag atttagtttt atggatgctg cattttgctt
    13921 aatgtctgtg gcttatgtag gcattggttt tatgttcttt tatgaaacga gatcagaagg
    13981 attacattac atattatatg cctttttaat tgtttggctt acagatacag gggcttactt
    14041 gtttggtaaa atgatgggta aacataagct ttggccagta ataagtccga ataaaacaat
    14101 cgaaggattc ataggtggct tgttctgtag tttgatagta ccacttgcaa tgttatattt
    14161 tgtagatttc aatatgaatg tatggatatt acttggagtg acattgattt taagtttatt
    14221 tggtcaatta ggtgatttag tggaatcagg atttaagcgt catttcggcg ttaaagactc
    14281 aggtcgtata ctacctggac acggtggtat tttagaccga tttgacagct ttatgtttgt
    14341 gttaccatta ttaaatattt tattaataca atcttaatgc tgagaacaaa tcaataaacg
    14401 taaagaggag ttgctgagat aatttaatga atctcagaac tccttttgaa aattatacgc
    14461 aatattaact ttgaaaatta gacgttatat tttgtgattt gtcagtatca tattataatg
    14521 acttatgtta cgtatacagc aatcattttt aaaataaaag aaatttataa acaatcgagg
    14581 tgtagcgagt gagctattta gttacaataa ttgcatttat tattgttttt ggtgtactag
    14641 taactgttca tgaatatggc catatgtttt ttgcgaaaag agcaggcatt atgtgtccag
    14701 aatttgcgat cggtatggga ccgaaaattt ttagttttag aaaaaacgaa acactttaca
    14761 ctattaggtt attgcctgtt ggtggatatg ttcgtatggc aggagatggc ttagaagagc
    14821 caccagtcga gcccggtatg aacgttaaaa ttaaactgaa tgaagaaaat gaaataacac
    14881 atatcatatt agatgatcat cataagtttc aacaaattga agcaatcgaa gttaaaaaat
    14941 gtgattttaa ggatgactta ttcatagaag gtatcactgc ttatgataat gaaagacatc
    15001 attttaaaat tgctagaaag tctttctttg ttgaaaatgg tagcttagtt caaattgctc
    15061 cgagagacag acaatttgca cataaaaagc catggccgaa atttttaaca ttatttgcgg
    15121 gaccgttatt taactttata ttagctttag tcctatttat tggtcttgca tattatcaag
    15181 gcacgcctac gtctactgta gaacaagtcg cagataagta tccagctcaa caagcaggat
    15241 tacaaaaagg tgataagatc gtccaaattg gcaaatataa aatatctgaa tttgatgatg
    15301 ttgataaggc gttagataaa gttaaagata ataagacgac tgttaaattt gaacgtgatg
    15361 gtaaaacaaa gtcagttgaa ttaacaccta aaaagactga aagaaaactg actaaagtaa
    15421 gttcagagac gaagtatgtt ctcggattcc aaccagcgag tgaacgtacg ctcttcaaac
    15481 caattgtata tggatttgaa agctttttga aaggtagtac actaattttt acagctgtag
    15541 tcggtatgtt ggctagtata tttacgggcg gattctcatt tgatatgtta aatggtccgg
    15601 ttggtattta tcataacgtc gactcagttg ttaaagcggg tatcattagc ttaattggtt
    15661 acactgcgtt attaagtgta aacttaggta ttatgaattt aattcctatt cctgcactag
    15721 acggtggtcg tattttattt gttatatatg aagcgatttt cagaaaacca gttaataaaa
    15781 aagcggaaac aacgattatt gctattggtg ccattttcat ggtcgttatt atgatattag
    15841 taacgtggaa tgatattcga cgatatttct tataatttag gaggataaat aattatgaag
    15901 caatccaaag tttttatacc aacgatgcgt gatgtgccat cagaagcaga agcacaaagt
    15961 catcgtttat tattgaaatc gggtttgata aaacaaagta caagtgggat ttatagttat
    16021 ttaccgctag caacacgtgt gttaaataat attactgcaa ttgtgcgaca agaaatggaa
    16081 cgtatcgatt ctgttgaaat tttaatgcca gcgttacaac aagctgaatt atgggaagaa
    16141 tcaggacgtt ggggtgcata tggcccagaa ttaatgcgtt tacaagatag acatggaaga
    16201 caatttgcat taggtccaac acatgaagaa ttagttacat caatagtaag aaatgaattg
    16261 aaatcataca aacaattacc gatgacatta ttccaaattc aatctaaatt ccgtgatgaa
    16321 aagagaccac gttttggttt acttcgtggg cgtgaattta ttatgaaaga tgcgtattca
    16381 ttccatgctg acgaggcatc attagatcaa acgtatcaag atatgtatca agcgtatagc
    16441 cgtatttttg agagagttgg cattaacgca agaccagtag ttgcagattc aggtgctata
    16501 ggcggtagcc atacacatga atttatggca ttaagtgcta tcggtgagga tacaatcgtt
    16561 tacagtaaag aaagtgacta tgctgctaat atcgaaaaag cagaagtcgt ttacgaacca
    16621 aatcataagt attctactgt gcaaccttta gaaaaaattg aaacaccaaa tgttaagact
    16681 gcacaagaat tggcagactt cttaggtaga ccagtagatg aaatcgttaa aacgatgatt
    16741 ttcaaagttg atggcgaata tattatggtt ttagtgcgtg gccatcatga aattaatgac
    16801 attaaattaa aatcttattt cggcacagat aatattgaat tagcaacaca agacgaaatt
    16861 gttaatttag ttggtgcaaa tccgggttca ctaggtcctg ttattgataa agaaatcaaa
    16921 atttatgcag ataattttgt gcaagattta aataatttag ttgtcggtgc taacgaagat
    16981 ggctatcact taattaatgt aaatgtaggt agagacttca acgttgatga atatggcgat
    17041 ttccgtttta ttttagaagg cgaaaagtta agtgatggtt caggcgttgc acattttgct
    17101 gaaggtattg aagttggtca agtattcaaa ttgggtacta agtattcaga atcaatgaat
    17161 gctacattct tagataacca aggaaaagcc caacctttaa ttatgggttg ttacggtatt
    17221 ggaatttcta gaacgctgag tgcgattgtt gaacaaaatc acgatgataa tggaattgtt
    17281 tggcctaaat cagttactcc atttgattta catttaattt ctattaatcc taagaaagat
    17341 gatcaacgag aactagcgga tgcactatat gctgaattta atactaaatt tgatgtgttg
    17401 tacgatgatc gtcaggaacg tgcgggtgtc aaatttaatg atgccgattt aattggttta
    17461 ccactgcgaa ttgttgttgg taaacgtgca tcggaaggta ttgtagaagt taaagaacgt
    17521 ttaacaggtg atagcgaaga agttcacatt gatgacttaa tgactgtcat tacaaataaa
    17581 tatgataact taaaataatt aagatcgaat gaattataag agtaggaaaa agctgaaaga
    17641 aatcagatgc ttatgtcctg ctcttattat ttttgatata atgattat
//
LOCUS       DAMZLE010000044        17485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMZLE010000044 DAMZLE010000000
VERSION     DAMZLE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.044"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(134..493)
                     /locus_tag="POB92_001312"
     CDS             complement(134..493)
                     /locus_tag="POB92_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDI6578820.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(506..742)
                     /locus_tag="POB92_001313"
     CDS             complement(506..742)
                     /locus_tag="POB92_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDI6578821.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            1059..2123
                     /locus_tag="POB92_001314"
     CDS             1059..2123
                     /locus_tag="POB92_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6578822.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(2182..2505)
                     /locus_tag="POB92_001315"
     CDS             complement(2182..2505)
                     /locus_tag="POB92_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDI6578823.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            2606..2848
                     /locus_tag="POB92_001316"
     CDS             2606..2848
                     /locus_tag="POB92_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDI6578824.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(3111..4286)
                     /gene="dltD"
                     /locus_tag="POB92_001317"
     CDS             complement(3111..4286)
                     /gene="dltD"
                     /locus_tag="POB92_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDI6578825.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(4283..4519)
                     /gene="dltC"
                     /locus_tag="POB92_001318"
     CDS             complement(4283..4519)
                     /gene="dltC"
                     /locus_tag="POB92_001318"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDI6578826.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(4537..5751)
                     /gene="dltB"
                     /locus_tag="POB92_001319"
     CDS             complement(4537..5751)
                     /gene="dltB"
                     /locus_tag="POB92_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDI6578827.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(5748..7205)
                     /gene="dltA"
                     /locus_tag="POB92_001320"
     CDS             complement(5748..7205)
                     /gene="dltA"
                     /locus_tag="POB92_001320"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDI6578828.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(7221..7373)
                     /locus_tag="POB92_001321"
     CDS             complement(7221..7373)
                     /locus_tag="POB92_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDI6578829.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(7421..7531)
                     /locus_tag="POB92_001322"
     CDS             complement(7421..7531)
                     /locus_tag="POB92_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578830.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(7785..8744)
                     /locus_tag="POB92_001323"
     CDS             complement(7785..8744)
                     /locus_tag="POB92_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDI6578831.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNQSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(8777..9556)
                     /locus_tag="POB92_001324"
     CDS             complement(8777..9556)
                     /locus_tag="POB92_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDI6578832.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(9556..9990)
                     /locus_tag="POB92_001325"
     CDS             complement(9556..9990)
                     /locus_tag="POB92_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDI6578833.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            10088..10354
                     /locus_tag="POB92_001326"
     CDS             10088..10354
                     /locus_tag="POB92_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDI6578834.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(10433..10816)
                     /locus_tag="POB92_001327"
     CDS             complement(10433..10816)
                     /locus_tag="POB92_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDI6578835.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(10946..11863)
                     /gene="lipA"
                     /locus_tag="POB92_001328"
     CDS             complement(10946..11863)
                     /gene="lipA"
                     /locus_tag="POB92_001328"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDI6578836.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(11947..13266)
                     /locus_tag="POB92_001329"
     CDS             complement(11947..13266)
                     /locus_tag="POB92_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6578837.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLKTLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(13293..14120)
                     /locus_tag="POB92_001330"
     CDS             complement(13293..14120)
                     /locus_tag="POB92_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6578838.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(14133..14981)
                     /locus_tag="POB92_001331"
     CDS             complement(14133..14981)
                     /locus_tag="POB92_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI6578839.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(15157..16224)
                     /locus_tag="POB92_001332"
     CDS             complement(15157..16224)
                     /locus_tag="POB92_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDI6578840.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIVSNIINQINQIMQYK"
     gene            complement(16238..17278)
                     /locus_tag="POB92_001333"
     CDS             complement(16238..17278)
                     /locus_tag="POB92_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6578841.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT         5516 a         3166 c         2561 g         6242 t
ORIGIN      
        1 taattttcaa taagcttttt aataaaacaa aatatcttta cgcaaaaata ggacataatc
       61 aaatatgtcc ttaatcgtta ttgatatcaa tttgtatccc aaaaattggg ttaaccatta
      121 aaaagttatt ttattagcaa ttttcaggat tacctgcaac ttttgcagtt ctaaatgaac
      181 taccacagcc acatgaagca attgcattag gattgtcgat ttggaaaccg ccacccatta
      241 atgattgctt aaaatcaata gtcgtaccat ttaatacggg tgcatctttt ttgtctacta
      301 atacttttaa tccaaagtat tctaagactt catcattttc accaggcgct tcttctgcac
      361 ccataccgta tgttaaacca gtgcacccgc caccattcac tttaattttt aaatagccat
      421 ctggcatttc atttgcttta agcatatctt ttacttcgta agcagctgct tctgttaata
      481 taactgttgg catgataact cctccttaaa aaatccaagt ttcttttata tgtgcatata
      541 tattttgtaa taattcttcc ggcgaatcac cttcaacaat atcaccattt actaaagcat
      601 acaacccggc tgaacatata ccacaatgtg tcaggcaacc atactctaac acatcgacat
      661 ctgggtcatt ttccagttga ttaaaaacat aatctccacc ttttgccatg ttagagagac
      721 aaaattctac gatcggattc atacttcacc ttcttatttc atttgttaca atattatagc
      781 attttaaaac tggtatttta acatgatgtg ctcaattagc aacaactgat gtttcttatc
      841 ccagttatgt aatagtgcct tagttagtac aaatatttat ttgtagtcat tttcaaaaaa
      901 atatataata agcatataaa tacatattta cttttttagg ggttattgat gaatcaaaga
      961 cacttgcatt ttataactgt tcattaacaa atatatctat gcactttgat cttttcaaaa
     1021 ttaaagtaaa tattaacata taaaaggggt acttcattat gaaaaactta gttttgttag
     1081 gcggcggata tggtaatatg cgtatcatgt cacgcatttt aactacttct ttaccacaag
     1141 attatacagt cacattagtt gatcgtatgc catttcatgg attgaaacca gaattttatg
     1201 ctttagctgc gggcacgaaa tcagataaag atgttcgtat gaaattccct aatcatccac
     1261 aagtgaatac agtttatggt gaaattaacg acatagattt agatgctcaa attgtctcag
     1321 tcggtaattc taaaattgat tatgatgagc taatcattgg tttaggatgt gaagataaat
     1381 atcataacgt tccaggagcc gaagaatata cacatagtat tcaaacactc tcaaaggctc
     1441 gggatacttt ccatagtatt agtgaactac cagaaggtgc taaagtcggt atcgttggtg
     1501 ctggattaag cggcatagaa cttgccagcg aattaagaga aagtagatca gacttggaaa
     1561 tatatcttta tgaccgtggg ccgcgaattt taagaaattt tccagaaaaa ttaagtaagt
     1621 atgttgcgaa atggttcgcc aaaaataatg ttaccgttgt tccaaattca aatattaata
     1681 aagttgaacc tggtaaaata tataactgtg atgaacctaa agatattgat ttagttgtat
     1741 ggacagcagg aattcaacct gttgaagttg ttcgtaactt gccgattgat ataaatagta
     1801 atggacgcgt gatagttaac cagtatcatc aagtaccaac atatcgtaac gtctatgtag
     1861 ttggtgattg tgctgattta ccacatgcgc caagtgctca gttagccgaa gttcaaggtg
     1921 atcaaattgc cgatgtgctt aaaaagcaat ggctaaatga accattacct gacaaaatgc
     1981 cggaactaaa ggtacaaggt atcgttgggt cattaggaga taagcaaggt tttgcatata
     2041 tcatggaccg cactgtaaca ggacgactcg cttctatttt aaaatccggc gtactttggt
     2101 tatataaata tcataatggt taaaattaaa aagcaatgtc agttcacact attactgaca
     2161 ttgcttttag ttatgtgtat attattcatt cacaagcttt tggtctataa atcgagtgat
     2221 ttgctttgtt tgtatataac catctgctac atattcatcg ttcattgtaa ttaatggata
     2281 aaatagttca tcttgttcta ttctttcaat aaattgtaaa tcatgatctg ttaagttgtc
     2341 attatctttt gtaatatcta tatacgtata tttaaatgat atatttgggt attttctttt
     2401 taatagcggc tgtagccagt cataaatatc tttcgatgtt ggcgcattaa cgcaacttgc
     2461 acatataaca tctgccccat atactaccac actcacgtgc tccatatatt tatcccccat
     2521 tggtttgata gatttttatt acactatcta ttataatata ctaataaaga ttatcaaatt
     2581 catctctcga aaggagactt gcctgatgcc tactgaagat acaacgatgt ttgatcaagt
     2641 agcagaagtt attgaacgtc ttcgtccatt tttattacgt gatggtggcg actgctcatt
     2701 gattgacgtg gaagacggta ttgttaaatt acaattacat ggtgcatgtg gtacatgccc
     2761 aagttctaca atcactctta aagctggtat tgagcgtgca ttacacgaag aagtgcctgg
     2821 tgtaatagaa gtagaacaag tattctaata taaagatatt aagttaacag aacatattat
     2881 gaataacaac aaagacagga atactgaatt atttataatc agttattacc tgtctttttt
     2941 cgctgtcaaa ttggactgat gggaaagtta ttttatacta tttatctgat taattatttt
     3001 attattgcca catatccaag cacatattac tattctattc tatgaaattt aaaatataaa
     3061 aatagcacat acgctcaatc aaaatcgttg agttatgtgc tatttgtatt ttaattttta
     3121 ggtttatcta cttcaggttg tggttcacct ttcatatgtt tcgcaatttg ctcatccata
     3181 taaacccaac ctttccaacc gatgtgtacg gcatcactga taacatattt ttcataatct
     3241 ttatcagtca tatcgtaaat tttaccacca ttatctacaa cagtagaatg gattttttta
     3301 taaactgctt gacgacgttc tttatcgata ccaatgtggt cataccatac accgtttgat
     3361 ggaatactta catattgaac atctgcacca gcagcacgca ttgtttttac aagtaattct
     3421 aaatcttgga attctggaga attaacattg aattcgtagt cacgtctaac tttacgctta
     3481 ctttcttgaa ttaatttcca gtattgatct ctaataccaa atttattcga tgtagtatca
     3541 gctttaccaa tttcaactgc tttttgtttc atctcatccc aagaagcgtc tggttttgta
     3601 gcaggtttaa catgttctaa tggagattta tccattgcaa acaatgattt aatcgcttct
     3661 attttaatca attgattctc tttaaaacca gaaatataac tatctttagt ttctttaggg
     3721 tttttagcat aagatttcaa gtattcttta ttgtgtacat gtggaaactg taataaacgt
     3781 tgtgcataac gacgttttaa ttcagtagac atgtttttct gctggaacat ttgattaatt
     3841 tgagtttgag acatacgagc atcaaagttt tgattcgtta aaccatggtt tgtaaaccat
     3901 tgtggtgaaa taataaatgt taatttctta ccttttaatt tatcatactg tgatgcaagt
     3961 tcaactgcgt taattaagtc tgtagaacca ccagcaccta ataagaatgc ttttttgttg
     4021 gcgttatgct tatttaatgc aattgcagga ttaaatgggt catctttacc taattcacta
     4081 gagccgtata taggataata cttgtttgat tcgtataact tatcttgaat gagtgtgcct
     4141 tttagtactt gatctgtcaa tgaagttcta ttatcttcta cagtcttttc atttactaat
     4201 cctgtaaacc aactagcagg taataataga aagacaatga ataccgctcc actaattaaa
     4261 atgggtaaaa aaggttttaa tttcatcgta actcttctaa tgcttcaacg attttatttg
     4321 gtgttgccca ctcatctcta tcaaagtcca taatagatac ttcgatatca agtttatttt
     4381 gaatctctaa taataatcca actgtttgga aagaatcaat aataccttct tcaaaaattt
     4441 ctacgtctgg attttctttt acaatatcat tttctgctac ttctgctaat aaatttaata
     4501 cttgttctct aaattccata attaaattct cctttattat ataagtttac ctgagaagat
     4561 taaaaagcca aatgttacaa agtggaatgt aatcacaatg ctaagtgctg ttgtgaaacc
     4621 attttgccaa cgtggcggat gtttcttacg ccaacgttca taatagccat aacctataaa
     4681 caatgctgca tggtataaac cataaacaat gtaatacact tcgataccat gccaaattcc
     4741 cattatgaag aagttgatta agaatgccac gttagacatt gcaaattgac tcttcaataa
     4801 ttttttacga gacatgtaga ataaagatct catgtaaata caatctctga accagaatga
     4861 taatgtcata tgccatctat tccagaaatc tttaatattt ttcgctttga aaggtttatc
     4921 gaagtttggt ggtgttttaa taccgaataa ataactaaat gctatcgcaa ataaactata
     4981 acctgcaaag tcaaagaata aatataagct gtatgcgtac atatataacc acaaattgac
     5041 aaagccatgt aagtctaatt gtaacggcat gattgcatat gtgttaataa agtaagcaac
     5101 aatatattta tacaagaaac caagcataat catgtgaatt gcttttaata ctaattcacg
     5161 atattcattg cctgttggta cttttttatc gtctttaacg aaacgtttgt aacgatcgat
     5221 tggtccagat gaaatcgttg ggaagaatga aataaattga attaatttcc atactttaat
     5281 ttctttgata gaaccatcac gaatttccat aattaactgc acacttttga atgtaacata
     5341 agaaattcct aagaaaccaa caaattcaat taatttactt tcatggaagt gaatctgatg
     5401 tccacctaac catgtacttt gtaacacttt cacaagtgct aatggcaata ttgataaaac
     5461 cattacagtt acaaattttg aaaatgaatt attttttggt tttgaatgat aataaaacat
     5521 tatcaataaa acttgccata cgacgtaaat aataaaacta attaattgaa cacttaaata
     5581 cttttggtca aacagattat gtttatcaga agaaaagatt aacacaatca taaatgctgt
     5641 aactacgcca ttataaatgt aacttcgctt acctaaaaat ccaagtataa tgactggtaa
     5701 taatgcaatt aaagcaatta agaagaatgt aaaatcacca tatggaatca tccgttaatt
     5761 acctctgcaa ttttctttct gtcaatttta ccatttgaag tcaatggcaa ttgttccatc
     5821 cattcaaact ttctaggaat catatactct ggtaagcgtg atttcaagtc atttttaata
     5881 tttttagtca tttctgcatt gtccgtaact tcagtcgttg gcacaattgc accaattaaa
     5941 tgaataactt tatcattttt atatacaggt acaacaatcg cttcttttac gaactcagac
     6001 tggcgtaatt gtgtttcaat ttcttctaat tccattctgt agccattcaa tttgatttgg
     6061 aaatcaatac gaccttgaat gaaccattga ccattttcaa acttcgcttt atcaccagtg
     6121 tgatatgtac gaataccgtc atcgaaatta aatacttcag ctgttttttg gtcatttttt
     6181 aagtatccta aacttacact ttgaccttcg ataacaagtt caccttcatc tgtagtagat
     6241 aatcttgcgc ctggtctttc aacgccaaca ggtaatgtcg gatattgatc taagatttct
     6301 tgtgtaattt gaatacttgt aactgctacc gtagcttcag ttggaccata tgtgttgtaa
     6361 atcgtcgcac ttgggaaacg gtttactaac gcttttgctg ctctgtgagg tagaatttca
     6421 ccacagaaga agaattcgtt aagactacca tattgttctt cattaagcgt tggtaataat
     6481 aaacacattt ccataaatga tggtgttgat acccaaatgt taatcggtgt tgctgttagc
     6541 atttcattta ataatttagg tttattaatc atgtttttat ctacaagatt taatgtaccg
     6601 cctgatgcta aacatggata aatagccatt acagataaat caaatgaaaa tggcgcttgg
     6661 ttaagccatt gttgttcatt tcctgattta ttaagttcta acatccactc agtaaattga
     6721 actaaacttg catattcaat ttgaacacct ttaggttccc cagtagaacc agatgtaaag
     6781 attgtgtata ctgtgtcgtt atctttaatc tgactatcaa aaattactgg gtcttgagat
     6841 gttttaatat cttctattgt aaatacttcg ccttctaaac tttcaaatga ttcatcagtc
     6901 gtattaaata caaactctgg ttgaaccttg ttaataatca ttttaatacg gtcttcagga
     6961 attgaagtgt ctacaggtac atatccacat cctgctttaa tggcaccaat catcccaaca
     7021 atcatatatg gtgacatgtg accgaataaa atcatcggtt tcttactacc ttgtaatcga
     7081 tgtgctaatt tactagactc atccattaac tgttgataag ttaattcatc agttgtgtgt
     7141 ctaacagcaa tgctttgtgg atttgcatcc gcaaacgctt gcagcttgtt aataatatct
     7201 gtcatattaa gtctccctca ttagaactca ttataaatga agttattgtg tgtgtcgcca
     7261 ctgccataaa ttaaatataa agtaataaat attgccaaat acaatagtgt taataaatat
     7321 ggtttgaatg cttcaacata tttattaggt ggctgtttac ttttagattt catattgcac
     7381 ctcttaaagt tcttagtaaa aacgccttta taaagaccgt tcaatataaa atacgtttta
     7441 aaatttgttt tttacaattc attatatcga tattcataat gaaattcaat tttaatttta
     7501 tagattcaac atagtaattg gttgtcatca ctcaattatt gtaaatgata cactttgttg
     7561 agaacatcat tcattttaaa ggttatttaa acaataaaca attacagtct atataacaat
     7621 tttgttatat acgtcaaaat caaataaact catcacatta atatgacgag tttataatgt
     7681 tattgaatta tcatcagcgc aaatatatac attcgcaagt caagcataac atatttaaca
     7741 attgctttgc ttgttttacc aatgattaaa aaccatactt attttcaatt tactggagta
     7801 tgtggtacct gatttgtcat aaccgcttct atattattaa tacataattg aatcatattg
     7861 tcccgtgttg ttactgatgc gctaccaatg tgtggtgtaa tcagaacatt atcacgtccc
     7921 attaatggat gtgtatgatc aatcggttca tttgctaata catctaaacc acatgctaaa
     7981 atttctttat tgtctagtgc atcgattaat gctgtttcat ctacaatttg tcctctaccg
     8041 atattaataa aaattgcatc atttttcatt tgttcaaatg cttcagcatt aaatttatga
     8101 tgtgtttctt ttgtaagtgg cgctgtacag atgataaaat cactttctgc taacaacgtt
     8161 tcaaaagaaa catatgttgc attaaagtcc gcctctgcat ctttatgtct tgattgatta
     8221 tgataaagaa tattagtatt aaacccttgc aaccttcttg caaaagcttt accaatatct
     8281 cccataccat atattccaat agttgaattg aagacatctt tacctgacaa taaataagga
     8341 ccccagcttt gccatgcatc tgcttctaca tatttttcag cttctacaat acggcgtgct
     8401 atagcaagca ttaatgtaaa tcctaattct gcagttgttt cagtaagtac atttggtgta
     8461 ttcgtgacaa ccacgttatt cgctgttgca ctttcaacat cgatgttgtc atatcctaca
     8521 gccatattcg caattacttt taaatttggt gattgcgcta aaatttctgc atcgatctgt
     8581 tcacttaatg taataaaaca agcatctgcg tcacgaagtg ctgtcacaaa ttgatcttta
     8641 ggcataggca ctaatgattt ttgccacata acaacgtcac caagtttact taattgttga
     8701 taaaatttat ctggaatttt cctcgaaaca actattttta ccaaatcaat tcacctactt
     8761 tataaaatga ctatatctat ttttctaatt cagctattgc ttcatttaaa tctttaaaag
     8821 aatacgttgg tggcacattt ttattttgca catcttctaa cgtagataca cctgtttgta
     8881 catgaatcgt atccatacct acgttaatac cagacataat atcggtatcg tacagatcgc
     8941 ctaccatagc aacttcggat ttatctaatc ctaaaatttc taatgcttta accataataa
     9001 tcggttctgg tttaccaata aattgtggcg atacacctgt cgatacactt acaacacttg
     9061 taatagcacc attaccaggt aataaaccac gctctttagg aattgataca tcaggatttg
     9121 tagaaataaa tgttgcacca tttcttacac ctaacgttgc aatcgcaagc ttttcatatg
     9181 taacttgttc gtcaagtcca ataactacat aatcaacatg ctcgtcattt ttaatgacaa
     9241 gtcccgcttc ggttaacgca gtatttaaac cactcccacc taacatatat actgatgctc
     9301 ctggtgattg ttctgaaata taatcagcag tggctaacgc tgacgttaca acctcttctg
     9361 gtttagcatc aatgtgcatt tcacgtaatt tttcagttac ttgctcaggt gtttttgttg
     9421 aattattagt tacgtataaa tgaggaatgc cttttacatt taaataatcg atgaattgtt
     9481 ttgctccatc aatctcatct gttcccatat acattgtgcc atctaaatcg attaaatacg
     9541 ctttatactg tttcattatt gattttctcc tttaccaaaa gctgttactg gtacattttc
     9601 ttgatgtaaa aatgtcgtta cttctgtaat aaattgtttg taataaggta atgcgtcatc
     9661 aaacattggc atgataatct taatatctaa ggctgtgtaa tcatatgtaa attgtttacg
     9721 aataccgaca gttttattaa tcgcctgctg tgtttcttta gtaataacat tttctagttc
     9781 taatatatca atcacatctt tataattacc aggatccctt aaaataaatg catcgataat
     9841 catattccct atatctaccg atgattctat caacatttga gcaatgcgtt caaatgcata
     9901 atgattgtgc ttgctctcat gataatcatc agttaatgct tgtaaatagg ctaatttctg
     9961 agttagttta tctttgtcta caaaatacat tccttgtcac ctctcactta tatattatca
    10021 taattttgca gtatacttca tttgtcgtta tactaacttt actactttta aaaggagtcg
    10081 ctacaaaatg attgatatgt atttatatga tgacaacgaa gagagtcaag ttcagtttgt
    10141 tggttttgtt ggcgagcata gtcgatatga tttgatgtta gtccatacaa atagacatta
    10201 tggtaagaca ctcgtactta atatgcaaac aaataaattc ggtattatcg gtactgatga
    10261 tttaaaagaa gaaggctata tcgctcatat tttaggtgta aatgctgaag aaggcgatga
    10321 aattactgag tatttaaatg aagtcattca ttaaagataa aaacccctaa ctagattaca
    10381 ggtccatgct tgaattgcat gattcccatt ttcaatctag ctaagggttt tattaatgtt
    10441 cagtatcttg aatggttggc ttaatcttca catcttgaag tttattgtcg tcatctatca
    10501 catcagacgg atgaacttct tcgattaact ttttaacctc ttgtttagac aaatgtctta
    10561 atacaaagta aggacaacca aagttacaat attcaaatat gtaatcttga atatttgaaa
    10621 aacgtttact catctctgct tttttattag aatctttgta aaaacctttt aatcgtaatt
    10681 gatcgtaacc atagtcacca actatgtaat catatttatc taaaatatct gaatacctag
    10741 caataaattg ttcttcatta aaacattcgc gataattttc tattaattca aagtaatgtt
    10801 gatctacttt tatcaaattc ttcacctatc tatgacaatg aaaggctcag tttatacttt
    10861 tagtgtttca taaaaataat gattacttct atgacacgtt cgttttgtac aaactaagcc
    10921 tttatgcctt attaatggtt aaatattaac tatttaactg tgcctcacct tggcgttgct
    10981 tttctttagc agcttcattt acttgctcat ccgcatgata agaactacgt actaaaggtc
    11041 cagcttggca atgtttaaac cctttatcca ttgccacttt tcttaattta ccaaactcta
    11101 aaggcgtgta atatttttga acctttaaat gtttacgtga aggttgtaaa tattgaccaa
    11161 tcgttaaaat atctacatca ttcgcacgta aatcatccat cgtttcataa atttcttcta
    11221 tagtttcacc taatccaacc ataatacttg atttagttgg gatatccggt tgtaattctt
    11281 ttgaacgacg taaaaactct aatgttctgt cgtaagtcgc acgcgcacga actctcggtg
    11341 ttaagcgacg aacagtttca atattatggt ttaaaatgtc aggtcttgac gccattaatg
    11401 tttctaacgc atcatagtcc ccgcccatat ctgatggtaa aatttcaatc gttgtaaatg
    11461 gatttctttc tctaacttta cgtactgtct cagcataaac atttgaacca gcatctctta
    11521 aatcatcacg cgcaacagca gtgataacaa cgtgtttcaa attcattaat tcaactgatt
    11581 cagctacacg ttcaggctca tttaaatcaa gttcattagg taaacctgtc ttaaccgcac
    11641 aaaaacgaca agctcttgta catacggcac ctaaaatcat aaatgtcgct gtacgacgtg
    11701 caccccaaca ttcatgtata ttaggacatt tagcttcttc acatacagta ttaagatttt
    11761 tttccctcat catcttctta agtcctgtat agttttcgtt ggtatttaat tttattttca
    11821 accaatccgg tttacgtaat atttcctcgt tttttgtcgc cataacaaca cataccctcc
    11881 tgttaagtgt atctttaaac attatatcga aattcatgac aaatgaaaat ccaataatct
    11941 tactttttaa tattccaata atttttcttt aaatatatct cttaaaaact ctggcattaa
    12001 atactctttt ggtaattctt tcaatgttgg gaaataacga ccgaacgtat acatatctat
    12061 cgtacctagt acatattctt tgtcgtcttc gatttcttct ccattcagat agtaacgccc
    12121 tgtagaatga atgtacccta aattataaag aatatatcct ccaaatatat tccctctgaa
    12181 acccaaacct tgtgcatgtt catacatata ttcttgttta ttacttttag ctataatctc
    12241 ttttaatttc acaccaaaaa gtctaaccct taccatatta atcggatgcg gtaacatttg
    12301 atgaatgtca tattctgtca cttcatcttt tacaatatct ttaacgagta agccagcatt
    12361 gatgatggca cattgtgcat gtgtatactc acaaacactt tgagctaata agtatgcagc
    12421 ttcagttatg tgattcatac tacgcttaag cactactgga tgttgaatta ctgaattgga
    12481 cattaacgtt tttccttctt cttcaaatga agtttcaact tcaggtaatg tttttaaagg
    12541 aataatcttt gcagttttat gtactacttt atgtgcctca aaagttaaat taacctctcc
    12601 aagataatta ccatactttc cagctgccgc catcagtaca ccattattga tttcaccatg
    12661 ttcaaaataa tgatgcgtat gactaccaaa aatgacatca atttcaggca attcttggca
    12721 taatgtttca tcgaagaaaa tgccacaatg acttagcacg attaatacat caaatttacc
    12781 tcgttgaagt tcaatttctt cttttataga ttcaagtgga tcggtaacaa tccaatttag
    12841 tgcacgataa aatggggtaa aaggtgctgt cgctgcaacg aatagtattt tcacaccgtc
    12901 tatgtcctta atataagaag aaacgatatt atttggtaaa tgacctgatt catctataac
    12961 attgctacat gtcactataa attttgcttc gtcgtaaagg tgatttaaag cttcgtgtga
    13021 aatggtcatc ccttcattat taccgattgt tgcaacatca cattttgctt catttagtaa
    13081 tgccacattc tttttaccta aagttgcttc agttataggt gcggataaat ctacatgatc
    13141 acctagatca acatataaag aaggatgatt aagtcgtggc cgatgttctg ccatatatgc
    13201 tttaatgcgt tcgtattcat gtaaatgact atgaatatcg ttcgtatgat aaattgtaag
    13261 cctcaaattg acgtcctcct tttaaagttg tattaaatca acgcacgaat aattaaatat
    13321 atacccatta acaacattac tgttctcaat aatgttacta ccgtatctga cttaattgat
    13381 tgattcactt tgacaccgat tttcgcacca aaataactag aaataattaa aatgattgca
    13441 taaccccaag ctacgtgacc ttgagcaatg tgccctatag aactcatgac acttgaaaag
    13501 aaaatcatca tcatacttgt tccaacagct acatgaggtg gaaatctaaa tacaataagc
    13561 attagtggcg tcattagtgc gccacctcca ataccaaata aacctgtcaa tataccaatt
    13621 aaaaacgttg taataaaagc aaacaatggt ggaacactat aatgatatgt tttaccttta
    13681 gcgtctacat aagtcttttc atacttgggt ttatcgaaaa ttttaaacgg tttaatctta
    13741 tttcttacca ttaataaaat ggctacgaaa attaaaaaga taccaaaata taaattaaat
    13801 gactcaaatg ttaaatatct actaatgaag gacccaagca atgaacctgg taatagtcca
    13861 aataaaaaga tggaaccatt tttaatatca acttgttttg tttttaaata tccaagtgat
    13921 gaagaaagtc ctgtcacaat tagaatgact gaagacgtcc ctatcgctac ttgtgttgta
    13981 atattatgta gtaatccatg ttcaacaccg aggtaaacca ttgttggaac gataataata
    14041 ccgcctccaa tgcctacgat agaccctata atcgctgaca aacctccgat taaaactaat
    14101 aatgtaattg ttaataacat agtcgtcgcc tcttaaaata attttaattg ttgtggtgct
    14161 aacccttcat attctatatt caataatcgc tgatatgttt tggcattatt tgctgcatga
    14221 ccaccagaat tattgttaaa tatgacgtaa actttcttag ctttttgtgc taatatttgt
    14281 gccttttgtg ccaagtctat taattcttgc tcattataat catataaata gcgtacatcg
    14341 cgccattctt gatctgacat atctttctta gtccaaccgt aatgattacg tccatgataa
    14401 cgtacaaacg caatttcatt tgtgattcga ttgactaaag gtacactgcc atctttcact
    14461 tgtggttcat caactactgc atgaatgatt tgatgttctg ttaaaaatgc caatgtttgt
    14521 tctttaaatg catcactaaa ccatgattga tgcctaaatt ctacacacat tggaaatgct
    14581 tgtaattgct gtcttacata caaaatatat ttgatatttt gtgcattgca gtcaaaccat
    14641 ggcggaaatt gaaccaatac cattgctaat tttttctgtg tatgtaaggg ctctaacata
    14701 ttcttaaatt gatcaaatag ttcttgcctt gtatctgcaa atgttttgta gtctgcatgc
    14761 aatgtgagtg cttgatgaat tttgaccaca aattcaaatg tatcaggcgt ttcttttatc
    14821 catttcaata tatttctttc cggttgtatc gcatagtatg tcgcatctaa ttcgacaacc
    14881 ggaaaatgtc cagcatatgt tttaagttta tcggtttggc gttctaaatc ttcatataat
    14941 gaatagtgat caccccaacc tgttaatccg atgtttatca tatatatcac caatgtcatc
    15001 ataccatata acttttatca taatcatttc agcgaacttt agtttgataa tttcgtttga
    15061 ctagattggt aaaatagaaa cgcaatccac aaaaatggca agcactaaaa taatgtttgg
    15121 gggtgcttgt gcttttgtgg attgcggtcg attcgattat ttatattgca tgatttgatt
    15181 aatttgattg attatattgg acacgatggt gttggcggga tgcgttgttg ctagtcgcgg
    15241 gctttgtcca ctccacatat gtgttaactc tttgtcgccg atgtttgccg cggcttttct
    15301 tatgctactt gttagctcat tttgtattgg ataatctggg atgtcgcctt cgtattggga
    15361 catttcttcg ataaacctat tgttgatacc gcgtgcaagc tttccactaa acgcttttgt
    15421 aacgactgta tctgtttctt tactatttat aattgcgtct cgtagtagtt ctgatgcatt
    15481 actatcttgt gatgttaaaa atgcagtgcc catttgtacc ccttctgcac ctaagacaat
    15541 acttgccaaa actcctctac cgtccataat tccaccagcg gcaatgaccg gaattgaaac
    15601 gacatctaca atttgtggca ctaaagatat tgttccaacc ataggtaatt gatttttagg
    15661 ttttaaaaat gaaccacgat gtccacctgc ttcactacct tgagcaacga tagcatccat
    15721 acccgctttt tcattcgcaa tagcttcatc aacacttgtt gctgtaccta ttagcttaat
    15781 gtttgcttct ttcaatcttt ttataataga ttcgcttgga attccaaaag taaaacaaca
    15841 tacaggcact tgctttttaa ttatcgtatc aatatgacac ttaaattgtt gttcttcggt
    15901 aatttttaca accggctctt ctaaatgtaa tgcgcgtcga taaggtttta accatgcatt
    15961 catattttca atttgactac tagtatatga ttgttgactt ggtacaaaga catttacgcc
    16021 aaaagaattt gacgttaatt ggcgtacata atctatttca tcttccaatt gctgcgtatt
    16081 aaagtaacct gcgcctattg tgcctaaccc accactgtta cttactgatg caactaattt
    16141 cggtgtcgta cttcctgcca tacctgcttg tataattgga tattcaatac ttaacatttg
    16201 agtaagtcga ttcttattcc acatagctgt tcgctcctta tatagatacg ttgcgatttt
    16261 tccgttgttg aaattgaatt tgctgttgag aaagtttttc tttttccttt ttatccatct
    16321 catcttcaat ttccatacct aataattctt caattaagtc ttcatgtgac actatcgctt
    16381 cagtaccacc aaattcgtcc aacacaattg ctaaatgttt tctagaaata gtcatcttac
    16441 gtaataccca ttcagcttta ttgtgttcat tcacaaataa tggcttagct gaatagtttg
    16501 taatttgatt ttctttttta ttactccaag ccaacagata tttagaatga aacactccaa
    16561 taatgttatc aatatctccc tcgtacactg gatatctagt gtatggctta ttcataaccg
    16621 tttcataaac ttcttcgtat gtcgcatttg aagcaaatgc cgtcacatta attctaggtg
    16681 ttgtatctac atcttttact tttaaatttt caaaattaat gacaccttcc aacctactcg
    16741 tctcaatttc atttaaagca ccttcatgtc cagcaattgc taacattgtt ttaaattctt
    16801 cttttgaaaa ttgatgttct tgaggttgac ccttagataa acttcgatta atactgtccg
    16861 tcaacttatt taaaagtaat gtgatagggc gaaacacaat gacacaaata ttaataattg
    16921 gatatacaag ccttgttatt ttatctggaa atgttgcagc gacagacttg ggaatcactt
    16981 cggaaatcaa aatgataaca actgttaaaa cagctgatgc aataccaacg ctaatccccc
    17041 aacgtaaagc cataattgta acaagtgttg gtaataaaat attcgcgaca ttattcccaa
    17101 ttagaatcgt tgtaataaac tcacttggtt tttcaagtaa ctttacaatg ccttttgctt
    17161 ttttatcacc tttgtcagct tcagttttaa attttgtttt attggcagcc gttaatgccg
    17221 tctcgcttcc tgaaaagaaa aacgaaataa atatcaatat aattatggca atgatcacgc
    17281 gtgtagtctc cttattgtca tatcttattt ttattgtagt tacttaattc ccgacgttct
    17341 cgtttaataa accagttaaa attcattata tataatagtc ttaataatga taattaatat
    17401 gcatttttag tcatagttac tttaatgcat tcattagtaa aatgatgcta tagttcgagc
    17461 tgtttgttat tattaaattt gcata
//
LOCUS       DAMZLE010000045        17480 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMZLE010000045 DAMZLE010000000
VERSION     DAMZLE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.045"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(125..682)
                     /locus_tag="POB92_001334"
     CDS             complement(125..682)
                     /locus_tag="POB92_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDI6578842.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(749..2389)
                     /locus_tag="POB92_001335"
     CDS             complement(749..2389)
                     /locus_tag="POB92_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDI6578843.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEHAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLTSKLHQSKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            complement(2662..4707)
                     /locus_tag="POB92_001336"
     CDS             complement(2662..4707)
                     /locus_tag="POB92_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDI6578844.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            5292..6347
                     /locus_tag="POB92_001337"
     CDS             5292..6347
                     /locus_tag="POB92_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDI6578845.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            6347..7243
                     /gene="murQ"
                     /locus_tag="POB92_001338"
     CDS             6347..7243
                     /gene="murQ"
                     /locus_tag="POB92_001338"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI6578846.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            7255..8709
                     /locus_tag="POB92_001339"
     CDS             7255..8709
                     /locus_tag="POB92_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6578847.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVILAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            8712..9587
                     /locus_tag="POB92_001340"
     CDS             8712..9587
                     /locus_tag="POB92_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6578848.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(9781..10062)
                     /locus_tag="POB92_001341"
     CDS             complement(9781..10062)
                     /locus_tag="POB92_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578849.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            10275..13064
                     /locus_tag="POB92_001342"
     CDS             10275..13064
                     /locus_tag="POB92_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6578850.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEVYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            complement(13211..13810)
                     /locus_tag="POB92_001343"
     CDS             complement(13211..13810)
                     /locus_tag="POB92_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578851.1"
                     /translation="MSLSKLPLLNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
     gene            13936..14691
                     /locus_tag="POB92_001344"
     CDS             13936..14691
                     /locus_tag="POB92_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HDI6578852.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDKVNLSITSTGKTGAGI
                     IKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELSKYG
                     VIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIKRIN
                     GDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            14688..15368
                     /locus_tag="POB92_001345"
     CDS             14688..15368
                     /locus_tag="POB92_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6578853.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGSNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTPSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILGGR
                     IQHEEVIK"
     gene            15365..16543
                     /locus_tag="POB92_001346"
     CDS             15365..16543
                     /locus_tag="POB92_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6578854.1"
                     /translation="MRKVLNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
     gene            16750..16971
                     /locus_tag="POB92_001347"
     CDS             16750..16971
                     /locus_tag="POB92_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HDI6578855.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
     gene            17009..>17480
                     /locus_tag="POB92_001348"
     CDS             17009..>17480
                     /locus_tag="POB92_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578856.1"
                     /translation="MGKPLFKKGDSMNNSIFYTYLKFNLRYFIRRKFEIVKRSRLNVT
                     TKILFLIKLIFSSLLLFISTIMLADYFSDYIDFIEYYFYASMFIYIVFIPLIPRTKLL
                     INPMDKQLLYRSSLREVDIFNLIYASDVLKKFVGYFNLLIVGMAISLYHISPFIF"
BASE COUNT         6078 a         2569 c         3322 g         5511 t
ORIGIN      
        1 gtaagttaag aacgatttaa cagatataga atatgttaaa tatagaaggt tagacaaccc
       61 gacgtattaa tactttgtga aataaagatc cccctcaaca tgatcgaagg ggggaagtat
      121 atgtttagtt caatatattt agaaagtcgt ttgtagttat tgtttgtccc atcaatggga
      181 atacattatc tattggaaat tgatgtagcg tttcgttttg tgcactcatc atatctgtaa
      241 caaaaaactg attgtagttt aattgatagg catctcgcgc tgtcgtatct acgccaatat
      301 gcgttgcgac accaccaaga acaatcgtat caattcctcg acgtcgcaat tgtaagtcca
      361 aatctgttcc tacaaatgca ctaaaatgtc gtttgtctat gacaaaatcg tcatctctct
      421 tgtctaataa atgatggaaa cgactgtagt cgtcgccttc ttttggtggt aatgagatca
      481 ttgcatttgg ttgcaataca tctttaccat catagaaatt cacgcgaaca aaagcgataa
      541 agccattgtt ttttctaaaa acatctatta atttattagc gttttgaacg acattttcag
      601 ctgtatatgg ggcataatcc attttaagaa taccttcttg caggtcgatt aacactaaag
      661 cggttttatt aaaattaatc atcttatatc agagtcctat tcgtttaata aagtattcgc
      721 ttaaacttat accctgtata cgatgaaatt atttattttg ttgtgaaaaa gctttagcga
      781 tatcgatgag tttcttcggt gcgtcttcga cagacatttt gacttcgaca aaatgcatca
      841 catcgggatg accattaatt gcattaaacg tgtcttgtaa atcttttgat gattcaacgt
      901 catgaatttc aacattttta ccaccaaata cagctggtaa agctttataa tcccacatgt
      961 gaatttcatt ataaggttca tacatgccgt gaataagtcg ttctaccgta tagccgtcat
     1021 tattaatcac aaataatacc ggtttaatat gctgtctaat catagttgaa atagcttgaa
     1081 cagttagttg caatgagcca tcaccaatta ataataagtt acgacgatct ttgtctgcta
     1141 attgtgaacc taatgttgca ggtaatgtat agccgataga accccataac ggttgcccta
     1201 taaaagtatt gtttttgtat aatgctaaat cataagcacc aaagaatgat gtaccttgat
     1261 cagcaatgat gacatcattt ggttttaaga aattttgcat cattttaaaa taagtttgtt
     1321 gtgttaatgg ttctgtgcca acagtataat cgggtgatgt tggacgatga tacgcaggga
     1381 acgttgcgtt attcgtatat gaaatattgg ataactgttt taacaatgat ggtagagata
     1441 tttcatcatt tgtaacatcg tcaattttga tattgtgatg atttaacata acgacatcat
     1501 cgatattgaa ttggtatgaa aaacctgctg ttgctgaatc tgttaatttg gctccaatat
     1561 ttaaaattaa atcgctgttg tccacataat ctcgtatttt atcttcggca attttcccat
     1621 cgtaaatacc catataatat ggattttcct cattaaaagc accttttcct aatgaaagtt
     1681 gtgctactgg tatctgtgtt tgatttacaa aatcttctaa ttcttgatga aggtgaaaac
     1741 tgttgatttc atgtccagta atgatgatag gctgcttcga ttgatgcaat ttagatgtta
     1801 ataactctat atatgttgat gcatccgtat ctttggctgc cgttacttca aatggtgtcg
     1861 gtatctcaat ttcagagatt gcgacatcga ttggtaaatg taaatgaact gggcgtcttt
     1921 cggcgattgc tgtattaatt aaacgtggta tttcggttgt tgcattttca ggtgtgatat
     1981 aaccttgtgc aacggttata tgtgcaaaca tttttcggta gtcgtcaaat gtaccttcac
     2041 caagtgagtg atgtacatat ttaccggcat gttcaacagc acgtgtcggc gcacctgtaa
     2101 tcgcaatgac aggtatgcgt tcagcatatg aacctgcgat accgttgacg gcacttaatt
     2161 cgccaacacc aaatgtagta actaatgcag cgagtccatt aagacgggca taaccgtccg
     2221 ctgcgtaact tgcgtttaat tcatttgtat ttcctaccca atctacattg ggattgctga
     2281 taatatcgtc tagaaaagcg agattaaaat caccaggaac accaaaaatt ttatcgacgc
     2341 ctgctcgatg aatagcgtca attaagtaag ctccaatgcg ttgtttcata aaaaaaccac
     2401 cttttctcta tatttacact taaaatgata gcctcgaata tagtgtaagg tcaaggaagt
     2461 tgactttgtt gtgaaaacgc taacaatagt gaacaattaa tattttttag gaaatgagta
     2521 tgaagggggg ctttaattga tgatcttaaa attcaaaatt taaggggaga gcgaactatc
     2581 aatgatgctg caaaatacaa tagcaaagaa aaaacaggcg cgtcacctta tgaaggtata
     2641 ggcacctgtt tatagtaagc attatttagc ttcaaataat tgatcgccaa atgaaatgtt
     2701 gccatgttca ccttgtttaa aatcaaggtt tgtaatgttt ccttgtgtca cgataatagg
     2761 cgtaatatca ctctttgcat gattgcggat gtagtctaaa tcaaagttga ttaataaatc
     2821 accttgttta acttcttgac cttcctcaac atgtaaagta aagccttctc cgtttaattt
     2881 aacagtgtct aaaccgatgt ggattaataa ttctaaacca ctatctgata caagaccaat
     2941 tgcatgtttt gttgggaaaa tcatttgtac tttaccgttg aatggtgcac gaacttcacc
     3001 ttgtgaaggt ttgatagcga taccgtcacc catcattttt tcgctgaaca cttgatcagg
     3061 cacttctgat aatggtgtta cttcaccagt taatggtgca tgcacgatat ggctcaattc
     3121 gcttgttgca gatttatctt ctgcaacaac aacagtttcg tctttatcgt cttccatagt
     3181 agtaggattt tctactactt gaccattcat aatctgttgc atttcatgtt tgatttggtc
     3241 agatttagga ccaaaaattg cttgcatatt attgccgact tctaatacac cagatgcgcc
     3301 taaatctttc aaaccaggaa catcaacttt agatttgtcg ttaacttcaa cacgtagacg
     3361 tgtgatacaa gcgtctaaat gtttaatgtt tgctttgcca cccatagctt ctaatactgc
     3421 atatggtaat tcagttgctg aagcagtagc cgcttgtgat tgtttatctt cacgacctgg
     3481 tgttttgtat tttaatttta caattaagaa tcggaatacg aagtagtaaa taactgcgta
     3541 tacaagacct acaggaataa ctaaccacca ttgtgtctta ttaggtagta taccgagtaa
     3601 gaagtagtcg atgaaaccac ctgagaatgt ataacctaga tgaagatcta ataagtacaa
     3661 tgttaagaat gataaaccat caagtactgc gtgaataaag aataataatg gtgctacaaa
     3721 taagaatgag aattctaatg gttctgtaat accagttaag aatgatgtta aagcagcaga
     3781 acccattaaa cctgctacta ctttcttatt ttcaggttta gctgtgtgat aaattgctaa
     3841 agctgctgca ggtaaaccga acatcataac agggaattca ccttgcatga atttaccagc
     3901 tgtcaaatgt gcgccttcac gaatttgttc gataaagata cgttggtcac cgtgaataat
     3961 ttcaccagct gcatttttcc atgaaccaaa ctcgaaccag aacggtgcgt ggaaaatgtg
     4021 atgtagaccg aatggaatta ataaacgctt gatgaaacca aataagaata cggcaacacc
     4081 agtatttgaa tctaataatc ctgtactgaa tgcatttaat cctgattgaa tcgttggcca
     4141 aattaatgcc attgggaatg ctaaaataaa tgatgttgta gccatcataa taggtacgaa
     4201 acgcttacca gcgaagaaac ctaaataaga tggtaagtta atgttataga acttgttata
     4261 acaccaagct gccagggccc cgattataat accgccgaac acacctgttt gtaatgttgg
     4321 gatacctaaa atgctagcgt aaccactcgc tggatcacca atattcttag gtgtaacttg
     4381 taaaaagtcg cccattgttt tgttcatgat tatgtaaccg acgaatgctg cgatagctgc
     4441 tacgccatca ccgccagcta atccgattgc gacacctaat gcgaaaatca taggcaagtt
     4501 atcaaaaatg ataccaccag cacctgtcat taatttagcg acagtttgta cgccaccatt
     4561 ttgtataaac ggcaagtagt gttgtaatga ttcaccttgc atagctgtac cgatagctaa
     4621 taacagaccc gctgctggta aaattgcaac aggtaacatt agcgctttac caatacgttg
     4681 caattgaccg aaaagtttct tcctcacttg tccaacctcc aaagttgtat gtaatattta
     4741 agattcaata aaaaagacac gagcaaatag actgtgttgg taaccatcac agcttaattt
     4801 aactcatgcc taatcttact tagtaacacg ttggtgtatg taattaatgt aaaagaagca
     4861 attgactaag tagtatataa aactgatacg ttagtttatc tagcttacca tcacatctta
     4921 ttgaatctta tttgtttgcg actcctattt tagcatggtg aagtatgcgt tttcaataca
     4981 attattaaat tcaatttcaa atctcttaat tattcacaga ttattattaa aacataatat
     5041 aagataatcc tgaataaaat gtataaaagt tattgtaata atccagcagt agcagctatt
     5101 atcagcactt aaaagaaagg ataatctttg atggtgtaat aattaacttg tgacagaaat
     5161 atgtaagatt attacaatga acaggcttta actgattaat attgtaatgt gttttctctt
     5221 atagtaaaat gtaagcgatt acacaactca actatatttt cctaataatt atattgttaa
     5281 ggagggctac tttgacaggc ttttcagtgt atttaggaca acctttagat gaagcgtata
     5341 ttaagcgaat gattaaacaa ggttaccaaa tgatttttac atctgtacaa ataccagaag
     5401 aagatgacga gacaaaatat cattatttca caaaactact caatttatta aaacatgaac
     5461 aagtgactta cctcatagat gctaatccat ctatattaac accatctttt tatgagcatc
     5521 ttcgacaata tgatgcgcaa tttatgattc gtatcgatca tagtacatca attgaggcaa
     5581 tcgaagcgat aatggcacag ggtttaaagt gctgtttgaa tgcaagtatt atttcccggg
     5641 aattgttaac aagcttacat caacaattga atgattttac attactttca ttttgtcata
     5701 actattatcc aagaccagat acgggattat ctgttgactt ggtcaataag aaaaatgaac
     5761 tcatttatca atttaatcca aaggcacaaa tatatggttt tattgtaggg agtgatttgc
     5821 gaggtccttt gcataaaggc ttgccaacaa ttgaagcaac gagacatagt catcctgtcg
     5881 ttgcagctaa attattacaa gaaactggtg tatctgaagt gttagttgga gactcattga
     5941 ttgaaatgag gcaggcaaaa caacttatag atttttgcaa gcataggcat ttcacgttat
     6001 gtattgaaga agtgtttgat acgacagtga cttacctttt cgatatgtgt cataaagtac
     6061 gcccggataa tccggaaaat gtcattcgtt cggaaacgtc aagacaaata tgtccacatt
     6121 cgattcaacc acagtttacg acgcaacgac gcattggttc agtaaccgtt gataatttga
     6181 ataacggacg ttatcaaggc gaaatgcaaa ttgtgagaca aacgcttagt gcacatgaca
     6241 atgtgaatgt tgttgcacaa attattaaag aagacttacc actgttaagt tgtatcgagc
     6301 cgaatgatac atttgatttt caaaaaacta gggagtgtaa gaagtgatgg aaaatagtac
     6361 gaccgaagcg cgtaatgaag cgacgatgca tcttgatgaa atgactgtgg aagaggcttt
     6421 aattacgatg aataaagaag atcagcaagt cccgttagca gttcgaaagg caataccaca
     6481 attgacaaaa gtaattaaaa aaacaattgc acagtataaa aagggtggac gattgattta
     6541 tatcggtgca ggtacaagtg gaaggttggg tgtcttagat gcagcggagt gtgtacctac
     6601 attcaatact gaccctcatg aaattatagg tattattgct ggtggacaac atgctatgac
     6661 gatggctgta gaaggtgcgg aagatcacaa aaaattagcg gaagaagatt tgaaaaatat
     6721 agatttaaca tcaaaagatg tcgttatagg aattgccgcg agtggcaaaa cgccatatgt
     6781 tataggcggt ttaacatttg ctaacacaat cggtgctaca acagtatcta tttcatgcaa
     6841 tgaacatgca gttataagtg aaattgcgca gtatccagta gaagttaaag ttggtccaga
     6901 agtattaact ggttcaacac gtttaaagtc tggtacagca caaaagttaa ttttaaatat
     6961 gatttcaacc atcacaatgg ttggtgtcgg aaaagtttac gataacctca tgattgatgt
     7021 taaagcaacc aatcaaaaac tgatcgaccg ttcagtgcgt attattcaag aaatatgtgc
     7081 tatcacatat gatgaagcaa tggcgttata tcaggtatct gagcatgatg tgaaagttgc
     7141 gacagttatg ggtatgtgtg gcatttctaa ggaagaagca acaagacggt tattaaacaa
     7201 tggtgacatt gttaaacgag caatcagaga tagacaacct taggagggat ttaaatgacc
     7261 aaagaacaac aacttgcaga acgaattatt gctgcagtag gtggtatgga taatatagat
     7321 agtgtcatga actgtatgac acgtgtgcgt attaaagtat tagatgagaa taaagtagat
     7381 gaccaagaac taaggcatat tgatggtgtc atgggtgtta tacacgatga acgtattcaa
     7441 gttgtggttg gacctggtac agtcaataaa gtggctaatc atatggcgga attaagtggt
     7501 gttaaactag gtgacccaat accacaccat cacaatgata gtgaaaaaat ggactataaa
     7561 tcatatgcag ctgataaagc aaaggcgaat aaggaagcgc ataaagcaaa acaaaagaat
     7621 ggtaagttga ataaagtatt gaaatcaatt gccaatatct ttataccgtt gattcctgca
     7681 tttattggag ctggattaat tggtggtatt gcagcagtac tgagtaactt aatggtggca
     7741 ggctatattt caggtgcttg gattacgcaa cttataacag tatttaatgt cattaaagac
     7801 ggtatgttag catacttagc tattttcact ggtattaatg cggctaaaga atttggtgcg
     7861 acaccaggac ttggtggcgt gattggtggt acaacgttat taacgggtat tgctggtaaa
     7921 aatattttaa tgaatgtctt cactggagaa ccattgcaac ctggacaagg tgggattatt
     7981 ggcgttattc ttgccgtttg gattttaagt attgtcgaaa agagattaca taaaattgtg
     8041 ccaaatgcga ttgatattat tgtaacgccg actattgcat tgttgattgt aggactatta
     8101 actatcttta tctttatgcc attagcaggt tttgtttcag acagtttagt ttcagtagtt
     8161 aacggaatta ttagtattgg tggcgtattt agtggattta tcattggtgc aagcttccta
     8221 ccgttagtta tgttagggct tcatcatatt tttacgccaa ttcatataga aatgattaac
     8281 caatcaggtg ctacttactt attgccaatt gcagcgatgg ctggtgctgg acaagtaggt
     8341 gccgcattag cactttgggt aagatgtaaa cgcaacacaa cattacgtaa tactttaaaa
     8401 ggtgcattgc cagttggttt cctaggtatc ggagaaccat taatctatgg tgtgactttg
     8461 ccattaggtc gacctttctt aactgcttgt attggtggtg gtattggtgg cgctgtaata
     8521 ggtggaattg gacatattgg tgccaaagca ataggcccaa gtggtgtgtc actattacca
     8581 ttaatctcag ataatatgta tttaggttat attgcaggat tacttgctgc gtatgctggt
     8641 ggattcgttt gtacatattt atttggaacg acaaaggcga tgcgacagac agatttgttg
     8701 ggtgattaat gatgacaaat attttatatc gcattgataa gcagttgagt gattttacga
     8761 agacagaaaa gataatcgct gattacattt taaagaatcc acataaaatc attgatatga
     8821 ctgtgaatga tttggcagat gttacgaatg ttagtacagc atcaattgtt agatttagtc
     8881 ggaaaatgac acatcaaggt tttcaagagc taaagattgc gatatctcga tacttacccg
     8941 aagatattgc aaccaatcca catttagaat tgattgaaaa tgaatctgta gaaactttga
     9001 aaaataaaat gattgctaga gcaacgaata cgatgcgatt tgtagctact aatattatgg
     9061 atgcgcaaat tgatgcaatt tgtgatgtgt tgaaaaatgc caggacaata tttttatttg
     9121 gatttggcgc atcgagtttg actattggtg atctttttca aaagttatct cgtattggct
     9181 taaatgtcag gttattacat gaaacgcatt tacttgtgtc aacatttgcg acgcatgatg
     9241 atagagattg catgattttt gtgacgaatc aaggtagtca tagtgaattg cagtcaattg
     9301 cacaggtggc cacacattac agtattccca tcataactat atctagtaca gctaataatc
     9361 cagtggctca aattgcagac tatgcattga tttatggcag aactgatgaa aatgaaatgc
     9421 gtatggcggc tacaacgtca ctatttgcac agttattcac ggtagatata ttgtactatc
     9481 gatttgtagc attaaattat catgcgattc tagattgtat aacccaatcg aaaatggcac
     9541 ttgataatta caggaagcat cttgcgacga tagattttaa acattagata ttgtgtgcgt
     9601 tctaacataa agttagttgc aacgtaaaca attgaagttt ttgatgtgat tcagttgctt
     9661 gatgaaaaat tgttgctaca agtttgtaaa actggcatag aggctatttt gaaaatgata
     9721 aaggcgtgac aaattaaagc aaagttcaga tttgagcttt gcttttttca tagatggaaa
     9781 ttaaatgctg aagaataatt ttaatgctaa atgaactaat acgaaggtgc taaaagcaat
     9841 tattatcaaa ttagtgatat gaatgaaatg tttaaatcgt ttgcgttgat tgtattcggg
     9901 acttttatag agtaggtagt agtacataaa gagtatggct gcgaacatgc cgaatattga
     9961 agtgaagtta cctacagtga ctgccatgta atgatttaaa gttaaatagt aacttagact
    10021 aataataaaa aataaaatga tttgataggt aattaacacc attcaagtcc ctccattaat
    10081 cgtagacaaa aaatttatca atgatttgat tatttgtatt caaattttag tatacgactt
    10141 acctcaaaga caaatattaa aagataagac aataaaatta gaataaattt acgtttgaag
    10201 ataaaaaaca aacatttttc attaaagttt atgatatatt tagagcaata gaaagtgtat
    10261 ggaaggggat atgaatggca taccaaagtg aatacgcatt agaaaatgaa atgatgaatc
    10321 aacttgaaca attgggttac gaaagagtaa cgatacgtga taataagcaa ttgcttgata
    10381 attttagaac aattttaaat gagcgtcatg cggacaaatt agaaggcaat cccttaacag
    10441 ataaagaatt tcaacgtctg ttaacgatga ttgatggaaa aagtattttc gagagtgccc
    10501 gtattttacg tgataaatta ccacttagac gtgatgatga gtctgaggtt tatttgtcgt
    10561 ttttagatac gaaaagttgg tgtaaaaata agtttcaagt gacgaatcaa gtatctgtcg
    10621 aggatacata taaagcacgt tatgatgtaa cgatattaat caacggacta ccccttgtcc
    10681 aagttgaatt gaaacgtcga ggtattgata ttaatgaggc gtttaaccaa gtaaaacgtt
    10741 accgcaaaca aaattacaca ggcttattcc gctacataca aatgtttatc attagtaatg
    10801 gtgttgaaac gcgatacttt tctaataatg atagcgaact attgaagagt cacatgtttt
    10861 attggagtga taaacagaat aaccgtatca atacattgca atcgtttgct gagtcattta
    10921 tgagaccttg tcaattagct aagatgatat cgcgctatat gattattaat gaaacagata
    10981 gaatactgat ggcaatgcgt ccgtatcaag tgtatgcggt agaagcactt attcaacaag
    11041 cgactgagac agggaataat ggatatgtat ggcatacaac tggaagtggt aagacgttga
    11101 cttcttttaa agcgagtcag attttatcac agcaagatga cattaagaaa gttatctttt
    11161 tggttgaccg taaagacttg gatagtcaaa cagaagagga atttaataaa tttgctaagg
    11221 gtgctgtaga caaaactttt aatacctcgc aactggtacg ccaactaaat gataaaagtt
    11281 tgccacttat tgtaacgacg attcaaaaaa tggctaaagc gattcaaggg aatgcccatt
    11341 tattagaaca gtataaaacg aataaagttg tatttattat tgatgagtgt catcgcagtc
    11401 aatttggtga catgcatcgt ctagttaaac aacatttcaa aaatgcccaa tactttggat
    11461 tcactggtac gccacgtttt ccagaaaata gtagtcaaga tggtagaaca actgcagata
    11521 ttttcggtag atgcttacat acgtatttaa ttagagatgc cattcatgat ggtaatgtac
    11581 ttggtttctc agttgactat attaatactt ttaaaaataa agctttaaaa gcagaagata
    11641 acagcatggt tgaagcaatt gatacggaag aagtatggtt agcggataaa cgtgtggaat
    11701 tagtaacacg acatatcatc aataatcatg ataaatatac acgtaatcgt caatattcaa
    11761 gtatatttac agtccaaagt attcacgcgc ttattaaata ttatgagaca tttaagcgac
    11821 ttaacaaaaa gttggaacaa ccgttaacga tagctggtat atttacgttt aaacctaatg
    11881 aagatgatcg tgatggtgaa gtgccatatc attcacgtga aaaattagag ataatgatta
    11941 gtgattataa taaaaagttc gagacgaatt tttcaacaga cacaactaat gagtatttta
    12001 atcatatttc aaaaaacgtt aaaaagggcg ttaaagatag taaaattgat atcttaatcg
    12061 ttgttaatat gttcttaact ggttttgata gtaaagtact gaacacttta tatgttgata
    12121 agaatttaat gtatcatgat ttaattcaag cgtattcacg tacaaatagg gttgaaaaag
    12181 aatcaaagcc atttggtaaa attgtaaact atcgtgactt gaaaaaagag acagacgatg
    12241 cactgagagt attctcacaa acaaatgata cggatacaat tttaatgcgc agttatgaag
    12301 agtataaaaa agaatttatg gacgcttatc gtgagcttaa aatgattgtg ccgacaccac
    12361 acatggttga tgacattcaa gatgaagaag agctaaagcg ctttgttgaa gcttatcgtt
    12421 tattagctaa aataatatta cgtttaaaag catttgacga gtttgagttt acaattgatg
    12481 aaattggaat ggatgaacaa gagaatgaag actataaaag taaatattta gctgtgtacg
    12541 atcaagtaaa aagagcgacg gctgagaaaa ataaagtatc cattttaaat gatattgatt
    12601 tcgaaataga aatgatgcgt aatgatacga ttaatgtgaa ttatattatg aatatattga
    12661 gacaaattga tcttgaagac aaagcggaac aacgtcgtaa ccaagaacaa attagacgca
    12721 ttttagatca tgcagatgat ccgacattga ggttaaaacg agatctaatt agagaattca
    12781 tcgacaatgt tgtaccttct ttaaataagg atgatgatat cgatcaagaa tatgttaatt
    12841 tcgaaagtat taaaaaagaa gcggagttca aaggatttgc tggagagaga tctatcgatg
    12901 aacaagccct aaaaacaatt tcaaatgact accagtatag tggtgttgta aacccacatc
    12961 accttaaaaa aatgattggt gatttgccat tgaaagaaaa gcgtaaagca agaaaagcca
    13021 ttgaatcttt cgtggcagaa acaactgaaa aatacggtgt gtaatgattc agccccctcg
    13081 ctagattagt gtagggggca ttattatttc attagaaaag gctcatttgt taaattcatc
    13141 ttatcaagaa tataacgaat gttccttgtt ttaaacttat taattaatag agacatttta
    13201 tctgaaaata tcactgaatg ctaatactaa tcaaagaaaa agtgataatt aaaataagat
    13261 atataatacc ccaaatagtg gatgctcgta tagaaagttt cgacgtacca attagcatga
    13321 ctgtaagtat gtatgcttgt acaaaaaatt gtaagtcgaa agtacccaga atgcgatttc
    13381 ctttagcaaa aatatttaaa ctagtaatag aatagtcaat taatgataaa ccggcactta
    13441 tttggatggt aagtataatt aaactaatag catttgtaaa cagtatgtag cttaacgttg
    13501 cagagaatat agattttgta ttagcatctg agcgcataat tctagagata actagaatga
    13561 ttaaaaatgt aaataatacg ctgaaaactc ttgagaaaaa aatactaatt tgactaaaaa
    13621 tttttatttg tgctaatgat gtctctgaga gtcctgcttc actcaataac tttttatagt
    13681 ccacagatag tccactaaca aatgtatatg taacaattat tatcaaagtc agaaacagct
    13741 taatgattaa ctttggttga atgcggatat ttgcaaagtg attaagcaaa ggtagttttg
    13801 ataatgacat atagacctcc taatgatata attctcctat attttatcat aaaaataata
    13861 taaaaaatat attttattaa aaataagtat aaaaaatggt ttgaagagta tttaacattt
    13921 ataggaggac ataaattgag gcgtaaaagg aaatggctaa ttgttatttg tgtattatta
    13981 gtattgatta cgatatcaca aattttgaaa cattttaact atggaactga gagtaaagaa
    14041 gcatacaata catatacggt tgaaaaagaa agacaattag atttacaagg taaggcatat
    14101 ccacgtgaag ttaaaacata ttataaaaat aatcaggttg gaacatattt gggtgtgcaa
    14161 gtagcggacg ggcaaacagt caaaaaagga gacaaagtta acttaagtat tacgagtacg
    14221 ggtaaaacag gagcggggat tataaaacaa atctctgagt tgcctattag ttatgaagag
    14281 aatttaagtc ttcacgaaaa tgatgccctg catttaccag aaagtaacaa cgatggggaa
    14341 ttagcaaatt caaaatcgat ttcggcatct ccaatattta agagtaataa caactctgaa
    14401 ttatcaaagt atggagttat aattgatgat cttagtctac caatacgtgc aggttattcg
    14461 ttggaaatta aagtcccatt aaatgcgatt aaaataccta aatcagtatt aacaaaaggc
    14521 aataatgttt ttattgttaa taaaaatagt attgtggaaa aacggaatat tcgtataaaa
    14581 agaatcaatg gtgacattat tgtagaaaag gggttaaaac ctggagacaa gttaattaaa
    14641 caacctaaaa gtacaatgaa tgatggtgat aaggttgaga tttcgtcatg atagaattaa
    14701 agaatgttta taggactttt aaaaacggtg ataaaaatat tcgagtttta aaagacatta
    14761 atatacatat caatgcaggt gaattcatag cgattatggg accatcaggc tcagggaaga
    14821 gcacactgat taatatctta ggatttatag atcgagagta tcatggtgaa tatttgtttg
    14881 aaggaagtaa ttatcagaca caatctgata atcaattggc aaacataaga aataaaacag
    14941 ttggatttgt ttttcaaaat tttaaactaa ttcataataa tactatttta gaaaatataa
    15001 gtatgccact ggtatataca aatatgaata cgatagaacg aaagaataaa gttatgactg
    15061 tgctcagtgc tgtaggccta gatggcaaag gtgattttac tcctagtaaa ctatcaggcg
    15121 gtcaacaaca acgtgttgca attgcgcgag ccattataaa taatccaaag tttattattg
    15181 cagatgagcc aacgggtgct ttggattcag atacatctaa aattattatg gacatattta
    15241 catcattaaa ccgagataat catactacaa ttattttagt cacacatgat cgtaaagtag
    15301 cagaaaaagc tgatcgaata attcatattt tgggtggtcg tatacaacat gaagaggtga
    15361 ttaaatgagg aaagttttaa atatttttac aatagctttt aaatcaatat tgaaaaataa
    15421 aggtagaaat atatttacaa tgataggtat aatcatcggt atttcttctg ttattacgat
    15481 aatgtctttg ggaaatggct ttaaaaaaac tgctgctgat caattttcag atgctggcgc
    15541 cggaaaacaa gaagcgttga ttagctttac ttttaaagtt gatgagaaaa tcaaaaagta
    15601 tccttttaat caaagagata tagagttggt aaatcaagtt gatggcgtac tagatgcgaa
    15661 gctaaaggaa aataaagaag aaggaattga agccacaata actaatgtcc agaaaaaaag
    15721 tgacatcttc attataaaaa aacaaaattt acactctttt aaagtaggta gagggtttga
    15781 taaagaggat aatgaattac gtaagaaaat agtggttata aatgatcaag tagcaaaaac
    15841 tgtattcaat aataatgcta ttggtaaatc gctatatatt gagggacagg gatttgaagt
    15901 cataggaatt acggataaac tatattctga ctcatctacc gttataatgc ctgaaaatac
    15961 attcaactac tatatgggac acttacatca aggtctgcct accttacaga taattattga
    16021 agatggttac aataaaaaaa ctgtagttaa aaaagtagaa tcactattaa ataaaaaagg
    16081 atcgggctct gttttaggtg agtatacata tacagacact gaagaaatta tcaaaagtat
    16141 tgataaaatt tttgatagta ttacttattt tgtagcagct gttgccggta tttcactttt
    16201 tattgctggt attggagtta tgaatgtcat gtatatatct gtagctgaaa gaacagaaga
    16261 aattgcgata cgacgtgcat ttggtgcaaa aagtcgagat attgaactac aatttttaat
    16321 agagagcatt ttaatatgtg tgacaagtgg ttttattgga cttattttag gtgttgtgtt
    16381 tgcaacaata attgatgtat taacaccaga ttatattaaa agtgtagtaa gtttgagttc
    16441 tgtcataatt gcggttagtg tgtcgatatt aattggactt ctttttggat ggataccagc
    16501 tcgcgcagca tctaagaaag agctcataga tattataaaa tagtaaattt aaaatggtaa
    16561 aagtactgaa gactaattat catattaaac tgactatcaa caccaacgat ggaaatgcgt
    16621 attagaataa aagtaagata tgtattgcaa aatgtaaatt atatatttaa tttacgcaaa
    16681 aaataacata ttacttttaa gatgtaaaaa aatattatat aatatttttg agaaggaggt
    16741 ggaaggccta tgctaaaatt acataaaaaa atagcttgga caggaatcaa aggttcagct
    16801 ataactacat ttattactgc cttatataat ggaagtagtg tttggaacgc gctagctgtt
    16861 gctggtattg cttttggtgg tggcgctggt actgcggttg cagctcttgg tcgtgcaaca
    16921 gtaatgagat ttatcaaacg ttggggcgta cgaaaaacag ctgcttggta aaagataatc
    16981 taattcttta taagagaggc tttctttttt gggaaagcct ctttttaaaa aaggagattc
    17041 aatgaataat tcaatatttt atacctattt aaaatttaat cttagatatt ttatccgtag
    17101 aaaatttgaa attgtaaaaa ggtcgcgact taatgttact acaaaaatac tgtttttaat
    17161 taaattgatt ttttcgtcac tattattatt tatttcaact attatgttag ctgactattt
    17221 cagtgattat attgatttta tagaatatta tttctatgcg agtatgttta tttacattgt
    17281 atttatacca ttaataccta gaactaaatt attgatcaat ccaatggata aacagctgtt
    17341 atatagatct agtttgagag aagttgacat ttttaacttg atatatgctt cagacgtttt
    17401 aaaaaagttt gttggatatt ttaatttgtt gatagttggt atggctatta gtttatatca
    17461 tattagtcct tttatattta
//
LOCUS       DAMZLE010000046        17439 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMZLE010000046 DAMZLE010000000
VERSION     DAMZLE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.046"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            379..3333
                     /gene="fdhF"
                     /locus_tag="POB92_001349"
     CDS             379..3333
                     /gene="fdhF"
                     /locus_tag="POB92_001349"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDI6578857.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKTH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3333..3806
                     /locus_tag="POB92_001350"
     CDS             3333..3806
                     /locus_tag="POB92_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDI6578858.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            4034..4267
                     /locus_tag="POB92_001351"
     CDS             4034..4267
                     /locus_tag="POB92_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578859.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4289..5065
                     /locus_tag="POB92_001352"
     CDS             4289..5065
                     /locus_tag="POB92_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDI6578860.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5790..6914
                     /locus_tag="POB92_001353"
     CDS             5790..6914
                     /locus_tag="POB92_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDI6578861.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNTYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6995..7948)
                     /locus_tag="POB92_001354"
     CDS             complement(6995..7948)
                     /locus_tag="POB92_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDI6578862.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(8310..8810)
                     /locus_tag="POB92_001355"
     CDS             complement(8310..8810)
                     /locus_tag="POB92_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6578863.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(9047..9469)
                     /locus_tag="POB92_001356"
     CDS             complement(9047..9469)
                     /locus_tag="POB92_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDI6578864.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
     gene            9717..10799
                     /locus_tag="POB92_001357"
     CDS             9717..10799
                     /locus_tag="POB92_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDI6578865.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            10894..12294
                     /gene="nhaC"
                     /locus_tag="POB92_001358"
     CDS             10894..12294
                     /gene="nhaC"
                     /locus_tag="POB92_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDI6578866.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            12468..12710
                     /locus_tag="POB92_001359"
     CDS             12468..12710
                     /locus_tag="POB92_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578867.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(12727..13536)
                     /locus_tag="POB92_001360"
     CDS             complement(12727..13536)
                     /locus_tag="POB92_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6578868.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            14146..16101
                     /locus_tag="POB92_001361"
     CDS             14146..16101
                     /locus_tag="POB92_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6578869.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMVKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIACSKIICDVDLINENNDLKEKITMSDFNKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            16128..16871
                     /locus_tag="POB92_001362"
     CDS             16128..16871
                     /locus_tag="POB92_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDI6578870.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLTLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            16935..17261
                     /locus_tag="POB92_001363"
     CDS             16935..17261
                     /locus_tag="POB92_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6578871.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYKVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
BASE COUNT         6120 a         2368 c         3230 g         5721 t
ORIGIN      
        1 tcaaacttgg gttattaatt ttcagaaaaa gggtatttat aaatagtata acgattttta
       61 ggtgaggtga ttattaaata ttgtcattaa taaggttgtg aaagatatgg caaaaagcgt
      121 gaaacagata attggaaaac taataatgat aattattttc aattaaacaa aaagatgatt
      181 taatttcgcg caaaatattt caaataaaaa tgattgtaaa aaggcaaaat acaaaatttc
      241 acttaacaac tagtacataa agtaatacaa ttaaattaat tctatctgaa agatgtgtgg
      301 ggcatcgtta ttttaggtgg atatgagcaa tttattaaaa gtcatttacg gaaaatatat
      361 atagacgggg tgagtaatat gcaagaacat ttggtggtta cacttgatgg aaaagattat
      421 cttgtagaac caggtacgaa tttacttgaa tttattaaat cacaagatac ttttgtacct
      481 tcaatttgtt ataacgagtc gatgggacca attcaaacat gtgatacatg tactgttgaa
      541 attgacggta aaattgaacg ttcatgtagt acggtgattg atcgtccaat gactgtaaat
      601 actgtgaaca atgatgtgaa agatgctcaa aaagaagcgc ttgatcgaat tttagaaaag
      661 catatgctgt attgtacagt atgtgattat aataatggtg attgtgaaat tcataatacg
      721 atggatgcat gggggcttca gcatcaaacg tatgaatata aagagaagcc atacgaaaaa
      781 gattacggcc cattttatcg ttatgatcca aatcaatgta tcttatgtgg gcgttgtgta
      841 gaagcatgtc aagatatcga agtgaatgaa actattagaa ttgattggga tcgtgaacat
      901 ccacgtgtta tttgggataa tgatgtaccg attaatgagt cttcatgtgt atcttgtggt
      961 caatgtgcga cggtatgtcc atgtaatgcc atgatggaag tgaatatgga aggtaatgcg
     1021 ggttatatga ctgatacgga acctggttca ttagcagcaa tgattgattt aactaaaaaa
     1081 gcagaacctg gttatggtcc actatttgca atctcagatt cagaagcaga aatgcgtaaa
     1141 gagcgtatta agaaaactaa aacagtgtgt acttattgtg gtgtgggttg ttcatttgaa
     1201 gtttggacta aagatagaga aattttaaaa gtacaaccat cacatgattc tccagcaaat
     1261 aaaattgcga cttgtgttaa aggtaagttt tcatggggac atattaattc agatcaacga
     1321 ttaactaaac cactagtaag gaaaaatggt gagttccatg aagtagaatg ggatgaagct
     1381 ttaaatgtca ttgcagataa ttttacatct attaaagaaa agtatggccc agatgcacta
     1441 tcattcattt cttcttctaa agcgacgaat gaagaatcgt atttaatgca aaaattagca
     1501 agacaagtta ttggaacaaa taacgttgat aactgttcaa gatattgcca agcacctgca
     1561 acaaaaggct tatttagaac ggttggacac ggcggtgact caggtagtat tgaagatcta
     1621 gaaaaagcgg caatgtctgt attgataggt accaatacag ccgaagcaca cccagttatt
     1681 gcatcacgca tgaaacgtgc tcaaaaatta tttggacaaa aaacacatgt gtttgatatt
     1741 agaaaacatg aaatggctga acgtgcggat cgtttttatc aaccgaaacc aggtacggat
     1801 ttagcgtggt taagcgcagt gactaagtat attattgatc atgatttaca cgataaagca
     1861 tttattgatg agtgggtaga tgattttgat gaatattaca aatcattaga aacatttaca
     1921 atggcttttg ctgaagaagc aacaggtatt cctgaatcag aattgattaa atttgctgaa
     1981 gaatgtgcta aagctgaatc tgttgtaatt tgttgggcaa tgggtattac acaacaagac
     2041 attggtagtg actcaagtac agcgatttca aacttattat tagtaacagg taattatcgt
     2101 cgtcctggta ctggtgcgta tccattacgt ggacataata atgttcaagg atgtagtgat
     2161 atgggaagta tgcctgataa gattactggt tatcaaagta ttgaagcgga tgatattcgc
     2221 gctaaatttg aaaaagaata tggcgttaaa ttgaatccaa aagctggtaa agataatcat
     2281 gaaatggtag aaggtataca tgacggagaa gtacactcat tgtacttata tggtgaagat
     2341 acgggtattg tagattcaaa tattaatttt gtacaagctg cgtttgaaaa attagatttc
     2401 atggtagtcc aagatgaatt tttaacattc acagcaacat acgcagatgt tgtattgcca
     2461 gcaagtcctt cacttgaaaa agacggtact tttacaaata ccgaacgtcg tattcaacgt
     2521 ttataccaag cattagaacc tcttggtgat tcaaaacctg actggaaaat tttccaagca
     2581 attgctaata gattaggatt tgattggaat tacaagcatc ctagtgaaat tatggatgaa
     2641 gtagcacgct taacacctct atatgctggg gtaagttatg atcgtttaga aggattcaat
     2701 agtttacaat ggccagtaca acctgatggt actgatgagc ctattctata cttagaagga
     2761 ttcaattttg ataatggtaa agcaaaatta ttcccattat catttgataa ttactttaag
     2821 caagatgaaa tttatgatat tcatgtaaac aatggtcgat tgttagagca cttccatgaa
     2881 ggtaatatga cttatcaaac accaatgatt aaatacaaag tgccacgtgc atttgttgaa
     2941 atttctccag agcttgctga agatagaggc attcatgaag gtgcagaagt taagctgatt
     3001 tctgaaacag gagaagcggt attacaagtt cacgtgacag atcgtgttaa aggtaaagaa
     3061 atctatattc cattaaataa tgatgcgatg gaaaatggag atctaggtgc aattaactta
     3121 ttaactaata gtgatgttga tcaatacacg gatacaccat cttataaacg tactagctgt
     3181 cgtttggagg taattacgaa acgtggtaag tcaccgttga accctaataa tttccgtgtc
     3241 aataaaaaac gtcatccgca gtacagtgtt caagtacaga aaaaatggga acgttcagat
     3301 tatgttttcc cgggaaatca ggtggataaa taatggctga gagaatatct tctaaaatta
     3361 gacgcttaga aaaatcagaa gaacaaatta agttagaaag tttaaatgaa gttacagagg
     3421 caattgcagc gaataaagat agtatattaa aagcaatcaa gcttattaaa acattagatg
     3481 atgcaaaact attagatgct ttaaatggtg caattcgagg acgtcaagtc atcataaata
     3541 aatttgcagt tgaacttaat aaagatattt atacagggct attatctaat atggcttcaa
     3601 tggtattttt attaggtgaa ctcaatgtat cagatttaag cgacttccta aataaagtta
     3661 ataaaggatt acacgttgcc aatcaagcga gtccaaatgc taagacaact ataagaagtc
     3721 tattgggcgt attgaaagat gacgacatga accgtagttt aacatacatg cttaatatgc
     3781 taaaaggtat gtctcgagaa gaataacatt acaatgaaaa agtgtttcta catgcaagca
     3841 gtgtaggagc acttttttta atttttgtaa aaattaaaaa gggtgttttt gatggaataa
     3901 atgaataata ttgaaaaaca aaaaaatcac gtgctattgt gaatcaatca tcgacaggcc
     3961 tatgaaacat catgtattat tatgattatg taagttattt aaataaaaag ggtgagtgca
     4021 tattaattcg agaatgttaa aaacgtcagc tgtaatttct aatacattat tagtcatagg
     4081 tttagtctgt ctatttatga tgaaattagt gttagctatt actttttttg ctgtttcatt
     4141 atcaattagt ctagttgtct ttaatatgat gttcagagat agaacaacga tgaaagtaat
     4201 cgttaacgct tcttttttaa tcgtaatatt agcgattgta gtggcatatt ttttattgtc
     4261 taagtaagaa aaatgaggtt ttgtctatat gaagaagaat ttcaagttac gcatttcaac
     4321 gctactattg atagttattt tagttgtttt tgctgtatta ctcatcgtga atgaaactaa
     4381 attgtttaaa aatgatgtga attactcttt tgatgaggct gtttcaatgc aacaagggaa
     4441 aggtattgta cagacaaaag aagaggatgg taaatttgtt gaagcaaata ataatgaaat
     4501 tgctaaagca atgactattt cacataaaga caatgatatg aagtatatgg atatcacaga
     4561 aaaagtgcca atgtcggaat ctgaagttaa ccaattgcta aaaggtaagg ggattttaga
     4621 aaatcgaggg aaagtttttc tagaagctca agaaaaatat gaggttaatg tcatttatct
     4681 tgttagccat gcattagtag aaacaggtaa cggcaaatca gaattagcaa aaggcattaa
     4741 agatggaaaa aaacgctatt acaacttttt tggtatagga gcattcgata gtagtgctgt
     4801 tcgtagtggg aaaagttatg ctgaaaagga acaatggaca tcaccagata aggcgattat
     4861 tggtggtgca aagttcattc gtaatgaata ttttgaaaac aatcaactga atttatatca
     4921 aatgcgatgg aatccagaaa atcctgcgca acatcaatat gcgagtgaca ttcgctgggc
     4981 agataaaatt gccaaattaa tggataaatc ctataagcag tttggtataa agaaagatga
     5041 tattagacaa acatattata aataagacat cggtgcttaa aggagctgga acaatttatt
     5101 gtttcgagct cctttagcac attctgagtg tgttagttaa atggatttta acctaacaaa
     5161 aaacgcggga gtgggacaga aatgataaag agccactaat gatttattat gtagtggttc
     5221 ttaaacatta gccacagcta atgtgtactt aaaaatagga atacatgagt aaaactcatg
     5281 cataagaaat actaatttct atagaaaaag tatttcttta tcgttgtccc accccaactt
     5341 gcattgtctg tagaaattgg gaatccaatt tctctatgtt ggggccccgc caacttgcac
     5401 attattgtaa gctgactttt cgtcagcttc tgtgttggga cccccacccc aactcgcatt
     5461 gcctgtagaa tttcttttcg aaattctctg tgttggggcc cctgactaga gttgaaaaaa
     5521 gcttgttgta agcgcatttt cattcagtca aatactgcca atataatatt atagacccta
     5581 ggacattgat ttatgtccca agctcccaca tcattgttac aaatgtacat gatgtaaatg
     5641 aatattgctg tctaaatgtg catgtaatat acaatggtgc agataataca cttaagtcct
     5701 taaaaatgaa acgttagttc caagagtcat ttttaaacaa tagtgcatgt gataaaatag
     5761 aaaagaatga aaaatataga ggtgacaata tgaagatagc aattataggt gcaggcatcg
     5821 gtggattaac agctgctgca ttattacaag aacaaggtca tactattaaa gtctttgaaa
     5881 aaaatgagtc agttaaagaa attggcgctg ggattggtat cggagataat gtgcttaaaa
     5941 aactaggtaa tcatgactta gctaaaggta ttaaaaatgc tgggcaaatc ttatctacaa
     6001 tgacagtgtt agatgacaaa gatcgcctgt taactactgt taaattaaaa agtaatacat
     6061 tgaatgtgac gttaccacgt caaacattaa ttgacattat taaatcttat gtaaaagatg
     6121 acgcaatatt tacaaatcat gaagtcacgc atatagataa tgagacagat aaagttacca
     6181 tacatttcgc ggaacaagaa agtgaagcat ttgatttatg tattggtgct gatggaattc
     6241 attctaaagt gagacaatct gtaaatgctg acagtaaagt attatatcaa gggtatacat
     6301 gctttagagg tttaattgat gatattgatt taaagcatcc ggattgtgca aaagaatact
     6361 ggggaagaaa aggaagagta ggtattgttc cgttattaaa taatcaagca tattggttca
     6421 ttacaattaa ctcgaaggaa aacaatcata aatatagttc gtttggtaaa cctcatttgc
     6481 aagcatactt taatcactat ccaaatgaag ttagagagat cttagacaaa caaagtgaaa
     6541 caggtatctt attgcataat atttatgatt tgaaaccact caaatctttt gtttatggtc
     6601 gtactatttt actaggagat gcagcacatg cgacaacgcc taatatgggg caaggtgctg
     6661 gacaagcaat ggaagatgct atcgtattag taaattgttt taacacatac gactttgaaa
     6721 aagcattaca gcgttatgat aaaatacgtg tcaaacatac tgcaaaagta attaagcgtt
     6781 ctagaaaaat cggtaaaatt gcccaatatc gtagtcgttt agttgttgca gttagaaatc
     6841 gtattatgaa aatgatgcca aatgcattag cagctggaca aactaaattc ttatataaat
     6901 cgaaagagaa ataatacaac aatatgaaaa cccccgtatg ttgaaacgag agctcaacat
     6961 atgggggttc ttgtttttat aataaattca attattagtt aacgacaaat tgtggtttct
     7021 caccttgaac ggcactaatt gcagcattag caacaatttt agacatcatg tcacgtgctt
     7081 caaatgtagc attaccaata tgcggtgtta atactacatt attaagtgat tttaagtcat
     7141 cggtaatatc tggttcaaat tcatatacat caagtgcagc accttcaatt tcattatctt
     7201 tcaatgcttg cactagtgct tgttcgtgca cgattggacc acgagaggca ttgattaaat
     7261 acgccgtaga tttcatcatt ttaaattgtt ctgtatcaat taaatgatgc attttaggat
     7321 tataagcagc gttgatagtg ataaaatctg cattctttaa tagtgtatct aaatctacat
     7381 attttgcacc gatttctcgt tctttttctt ctttgcgatt aggtcctgtg tatagtacat
     7441 ccatgtcaaa tgctcttgca cgacgcgcta ctgcactacc aatttcacct aaaccgataa
     7501 tgccgattgt tttcccagat acttctctac ctctgaaaaa taaaggtgcc catccatcaa
     7561 atccagttgt acgtgataat tggtcccctt caacaatacg acgcgctact gcaagtacta
     7621 atccaattgt taaatcggca gtcgcgtttg ttgatgcttt aggtgtgttt gtaacatcta
     7681 tacttttttc tcgggcatac tcgatatcaa tattattaaa accagcgcca tagttggcaa
     7741 tgatttttaa gtctttacca gcatcgataa catctttatc aacgtttgta gataataaac
     7801 taattaaggc agtcgcgttt ttaacacctt taattaaagt gtctttatcg actaatcctt
     7861 taccttcata catttcaact tcaaaatgtt cttgtaaaag ttttaaacct acttctggta
     7921 ttgcaccagc aacataaact ttttccataa aagatcactc cttttatctt agtatagtag
     7981 aagattagac agtatacaac tatgtcatga tgtcttgtgt atcaatgatg taagcgcgta
     8041 cttttgatgg aggtgatata acttaggcac tgtagaacta tgaatattgt aatgtggaaa
     8101 aactggatca attaaattag ataacgtagt tttaaagtta atagtattag aaaaaattaa
     8161 tattttgaat atgggaggaa atataaataa gtaggtggca acgaaaaata gcaaaaaaag
     8221 agcttctcct ataaaggaaa gctcaaagtt ttttgatgac atatgtacta gaattaagtt
     8281 tcaagacaat atgtatcatc gtgtttatat taaatatgga tgtagttgta gttacctgct
     8341 tcacttgcag aaatagttct agaacttact gagaaaggtc cgccactata attcatttct
     8401 gaaattgtaa ctgaaccatc actgtttaca ctttctacat atgcaacgtg accaaatggt
     8461 ccttcagaag attgtaggat agcgccttta gaaggtgtat gatttactgt gaatccagca
     8521 ccttgtgcag cagcagccca attattagca tttccccaag tagaaccgat ttctccgcct
     8581 actttatcat atacatacca agtacattgt cctgcagtgt ataagttacc agaatgtgaa
     8641 attgatgatg tagttgtcgt agttgttgta gtcgttgtag tttgagtcgt gttgtagtta
     8701 tagttgttgt aatttgtata attttcagca gcatctgcat gatgtgcttg acctactaat
     8761 gctgtgccga ttcctgctgt taacgtagtt gctgttacta attttttcat gaataaagtc
     8821 ctccaaagtt ctatatcttt ttttataaat aaaacgtagc gactgtttta ttctcacatc
     8881 tcgaattgat gacaatagtt actttaacaa aattaatgct tcttgtgggg aatgttattg
     8941 atttgtaaaa gaataaaaaa actttgacta attttgtaat aaaaattagt caaagttaca
     9001 atgagattaa cagataatta ataggaaata tttatttgta atatgtttaa ataaatcgaa
     9061 ttgttaaagg tattacatat tcttgaccat tataatattt gacacacgca ataattgtga
     9121 atacaaaaga taatattgag aaagcgaata tggataaaat accgataaat gtaatgatga
     9181 aacctataat gataatgaaa ctaatatcgg tagcaattag gaaaacgcct attaaagtga
     9241 taacgactaa aacgatagac caaataatat aagaaatcgt atagttaaga taattttttc
     9301 cagcacgatc aactagtttc gattcatctt ttttcaataa ccatataatc agtggaccaa
     9361 taatagatgt gaataaactt aataaataga taagcatcgc cataatgttc tcatcattgg
     9421 atttgcgatt cggttgatga tttgttacgt cgttcatttc agttgtcata ttagacactc
     9481 ctttgaaaat tgtaatatta tctttaacta taacaaaata taatcaaaaa taaacatgtt
     9541 tattaaacaa ttattaaaaa taaaaataat tggtggacgt cggcgtttaa ataggttaat
     9601 ttaaggttat atatacttaa catttataat gatgcgtaat gaattcgcat catttttata
     9661 ttgtcttacg tataatttgt ttttaatttt aaccaaagat agaaagaggg ttgtttatga
     9721 aaatagcaat tgtaggatca ggaaatggcg cagttacggc agcagtagat atggtgagca
     9781 aaggccacga tgttaaatta tattgtcgta atcaatctat aagtaagttt caaaacgcaa
     9841 tcgaaaaggg cggatttgat tttaataatg aaggtgatga acgtttcgta aaattcactg
     9901 atattagtga tgatatggaa tatgttttaa aagatgctga aattgttcaa gtgattattc
     9961 catcttcata catagagtat tatgctgatg taatggcaga gcatgtaact gataatcagt
    10021 tgatattctt caacatggct gcagcaatgg ggtcaattcg ttttatgaat gttttagaag
    10081 atagacatat tgaaacaaaa ccacaactag cggaagctaa tacgttgacg tatggtacgc
    10141 gtgtcgattt tgaaaatgca gcagttgatt tatctctaaa tgtacgtcgt atcttctttt
    10201 caacatatga tagaagctgt ctaaatgatt gttatgacaa agtttcaagt atttatgatc
    10261 atttagtaaa agaggaaagc ttaattaaaa caaatcttga aaatggtaat cctgaagtgc
    10321 atccaggacc aacattattg aatgtcggtc gcattgatta tgctggcgag ttcgctttat
    10381 ataaagaagg aattactaaa catacagtta gattacttca tgcaatcgaa ttagaacgtt
    10441 tgaatttagg ccgtagatta ggttttgaat tatcaacagc taaagaatca cgtattgaac
    10501 gtggttattt agaacgtgat aaagaagatg aaccattaaa tcgtttgttt aatacaagcc
    10561 cagtattttc acaaattcca ggaccaaatc atgtagaaag cagatattta actgaagata
    10621 ttgcatatgg tttagtacta tggtcaagct taggtcgtgt tattgatgta ccgacaccaa
    10681 atatagatgc agtaattgta attgcatcaa ccattttaga gagagacttc tttgaggaag
    10741 gcttaacagt tgaagaaatt ggtttagata agcttgattt agaaaaatat ttaaaataaa
    10801 tgatggcttg aagatagaaa aggatatagc attatgcaaa agcaataaat tgaagaaaag
    10861 aggtttctca tcaataagcg aaggggacga tagatgatga caagaaaacc aaccttttta
    10921 gaatcaattt cgacaatgat tgtaatggtt attgttgttg taacaggctt tgtgtttttt
    10981 gatattccaa ttcaagtatt attaattatt gcctcagcat atgccacatg gattgcaaaa
    11041 cgtgtaggct taacatggca agatttagaa aaaggcattg cagaacgttt aaatactgca
    11101 atgcctgcaa ttttaattat actagcggta ggaattatag taggcagttg gatgttttct
    11161 ggcacagtgc cagccttgat ttattatggc ttagatttat tgaatccaag ctatttttta
    11221 atatcagcct tttttataag tgctgttaca tctgtagcaa ctggtacagc atggggctct
    11281 gcatcaactg cagggattgc acttatttct attggtaatc aattggggat tcctccaggg
    11341 atggcagcgg gtgctattat agcaggggct gtgtttggcg ataaaatgtc accattatca
    11401 gatacaacta atttagcggc gcttgttact aaagttaata tatttaaaca tatacattcg
    11461 atgatgtgga cgacgatacc tgcatcaatc ataggtttat tagtatggtt tattgctgga
    11521 tttcaattta aagggcattc aaatgataaa cagattcaaa ctttgttatc agagcttgca
    11581 cagatttatc aaattaacat atgggtctgg gttcccttaa ttgtgatcat tgtttgtttg
    11641 ctatttaaaa tggctacagt gccagctatg ctaatatcaa gcttttctgc cattatagtg
    11701 gggactttta atcatcattt caaaatgaca gatggtttca aagcaacatt tagtggtttt
    11761 aacgactcaa tgatacatca gtctcatatt tcatccagtg tgaaaagctt gttagaacag
    11821 ggtggtatga tgagtatgac ccaaatatta gtaacgatat tttgcggata tgcatttgca
    11881 ggtattgtag aaaaagcagg atgtttagaa gtcttattaa ctactatttc taaaggcatc
    11941 cattctgtag gaagtttaat atgtattact gttatttgtt gtattgcgct tgtattcgct
    12001 gcaggtgttg cttcgattgt aattattatg gtcggtgtgt taatgaaaga tttgttcgaa
    12061 aaataccaag tctctcgttc cgttttatca agaacacttg aagactcaag tacgatggtc
    12121 ttacctttaa taccatgggg aacatcaggt atttactata cgaatcaact tcatgtctct
    12181 gttgaagaat ttttcatatg gacagtacca tgttatttat gcgcaattat agcaattatc
    12241 tatggtttta cagggatagg tattaaaaag tcatcgaatt cacgtttaac ttaatgtgag
    12301 cgtggaatat atataatatg ttgaaacact ttaatcattt ataattgtag cggttatagt
    12361 ttgaaaaggt tttaacttag aataaatatc ctctatacat atactgaata tgttttgtag
    12421 cggaacatgt tgatatatgt aatgtaagtt ttatgtcatg atttgtaatg actaaattaa
    12481 ttgagaattt gaaggcaagt atatttgtaa gtactttaac taaaaattta tcaatgtata
    12541 gccgatttga catgcctaaa tttgggtgtg tcaatggctg tatgttgttt attctttatt
    12601 acagagtgaa tcggattggt gaaaatcgaa attttgagat ttttaccaat tcgatttttt
    12661 tcatagaaat taaaaaagcc aacaaggctc ttgaaacctt gttggcgtaa acatagccat
    12721 cactaattag tgaatgaagt tataaccagc agcttggcta gctgagattg tacgtgaagt
    12781 tacaacacct gggccataac catagttcat ttctgaaact cttactgaac cattgctgtt
    12841 aacactttca acgtatgcaa cgtgaccgta tgcaccttga gttgtttgca taattgcacc
    12901 agcttttggt gtattgttca ctgtgtaacc agctcttgca gctgcgttag cccagttact
    12961 tgcattgccc caagttgaac cgattttacc acctacacga tcaaatacgt agtatgtaca
    13021 ttgaccagaa gtgtataagt tacgtcctga agtataacca cttgagattg aacggccatt
    13081 tgatgatgga gccatagttg tagttacttg aacattgttg cttgaagtgc tgtagcttgc
    13141 acctaaacca ccagtacggt agctgtttgt gttgtaacta ttatagttat tgtagttata
    13201 tgattgatta ttatttgagt agttgttgta acggctgtaa ttattgtagc tataaccatt
    13261 gttgtaattg ttgtagttat agttattgta gccattgtag tagtaatagc tgtagtagcc
    13321 attatcttgg tttaattgac ttggatgcca gttacctttc catgtgtaat ggtagttacc
    13381 ttgtgcatca atagtgtaag tatagctata tgatgttggg tcgtttggat tataaccgta
    13441 gttatcttgc tcagaagcat gagcttgatt tcctgatgca attgcgattg tagcgaatcc
    13501 tgcagttgcg atagtagctg tagcgatttt cttcatttta aaaatatcct cctaaaaatt
    13561 ttaaatctaa aatattttcg taatgtccgt gtgacaaaat taatgttata agttatctct
    13621 cgtaattaaa cgacaagaaa gactataaca gaaattagcg tccttgtgtg ctttgttaac
    13681 gttttgtaat tttttgctaa tatcttgaca caatagaatt ttaaaagtat agaaatttgc
    13741 attttgcaaa acttataact acggcattct ttgtgaaaac tgaatgtttc gaaaataagt
    13801 ctgttacaaa tttgtaatat tactgaaaat tctaaatgta tattttgtgc ataatatagg
    13861 acttttaatc agaaaatgca taaaaattcg acacgaacga tcaagtttct atgattatga
    13921 aagctttggt atttggatga aaatgtatat ttaatggata aaatatccta atttagcata
    13981 aaaaaatgtt ttaataaaag tattatttga tataatcgat ttatgttttg ttactgctaa
    14041 aaaacatgtg gcgatataat tggtagtgaa tattagatgt tgacaattga tttgagaaag
    14101 acaaacagga gtaaatcaat atatttgtat atgtgaggat gtaagatgac agataattat
    14161 tcactacata tttataaaag tatgaaaatg agtagtgtga aggaagcgaa cttaaaaatt
    14221 atatattggc tacaaggtac aggtgtagtt tcaatcaatc tacaacaata tgatgtaaaa
    14281 agaaatgatg tcaccgttat tatgttgaat gatttgtatc aaattgatgg tcatgatgat
    14341 agtgtatgtt gtgttgttga agtaagtgca aagacatttt tgaagtttat gaatgcaaac
    14401 tatatgatgc gtggcaagat attaacaaat gaggttgcta gcacttttag aatcttgatt
    14461 aagtatttaa tctacagcaa aattaaacaa cagtcttata atgctaatga taagattatt
    14521 aattacatgt gtgttgaatt aatgtcatta aatacatacg atggtagtcg tcatttagta
    14581 gccgaagaag tgcacgatta tttaacaaac aaccaccata aaaagataaa taggaaagat
    14641 gtcattaatc aagttagtat tacaaacaaa gctttaagtg acatggttaa agcgacacct
    14701 tatagtaatt ttattcaata tttaaatcat attagactgg agcattgctt aattgatatt
    14761 ttaacttcta agaagcctat tgaagaaatc gcaagtagtc atggttttaa tcattattct
    14821 cgttttatac acctctttaa agaaacgtat ggtaacactc ctaaattaat tagaaaaaga
    14881 tatagtccaa catctcattc aattaaccat tcccaattag ttgaaattga taaaaatata
    14941 attgaattat ttgatgatac taatcaaagt tcttcaagta tgcgcgagat tgatatacct
    15001 tttgagccta caaaaaggag tcaaatgtat caacctaaaa acatttatat taaaggaagt
    15061 aatttttatt ggatggatta ttatactatt aaacaaatca ttcaaagatt aggtgttaat
    15121 cctgaaaact tgcatataat tgttacaatc gacacaagca atgagctatc gattgaacaa
    15181 atcactttat tacttcaaaa gatagtgaat tacaatgtaa atgtagtatt tcgtattaaa
    15241 catgaatata aggaattgtt aacgagtact gaaagaggca agatagaaaa gctagtagct
    15301 acaattttta atatgacttt tgagagtaat agatgtaaga ttgcattttt aattaacgac
    15361 ttaaatacta ctgcaattca aaaactgaaa cgtttaatag ataattatat aggagtattt
    15421 gaattaatat atcatctgga accaaaagag ttgaatacgc gctatgataa agaaattgat
    15481 catctaatag actattttat cttaccgcta agacaaatag aacaggtgtc tattgcatgt
    15541 tctaaaatta tatgtgatgt agatttgatt aatgagaata atgacttaaa agaaaaaatc
    15601 acaatgtctg attttaataa ggttactgaa cttttgacaa attatacaaa atttcgagga
    15661 cttattatgc acatgtcaat tataagtgaa gcgagcaaag aaataaattt aactagatta
    15721 tcaatattca tttttatcat aaatatactt aatcaattac gagggattgt tatttatcaa
    15781 aatgacacga tgattgtaac gaagttcaaa catgaagtac aatgtgtcat ttgtttgcca
    15841 gccgcattgt taaatgagaa ttcagacgct atacgtttat cgtgtaaggg tcttcaacaa
    15901 tttagccatg ctaaaactaa acaaattgtt ttaagtatag ataaagataa tcatacttcc
    15961 gaaaatggtg tacttgtgaa tgatattatg caacaacaca gttattggaa agctaatttt
    16021 atagaaagaa atatttatga tcattcatta atggtctcaa gcaatagtat tgttcatttg
    16081 aaatatcatc ttgagatata ggcatggttt aagagaggtt tttaaagatg tctaaattta
    16141 aagttaagcg tttaactgca cacatactaa tgttgaaagt cattaatgat atgttgaaat
    16201 attcatttca tctcactttg actcaagtga aactattaaa tctattagtt aaatatgata
    16261 attctgattc caatagcgct tcaatagata gtttattaaa aatgaaagaa atccaatcta
    16321 agatggccct tcttcaaatg ttaagttatt taaacaatca tcaatggcta ttgaaaggaa
    16381 gagatcaaag ggatcaacgt aaattgacta tttcgattaa taaaatgcat atagataaaa
    16441 tagaatatat gaataatgag cttagtgact atattgaacg ttattttggt caatatacat
    16501 ttgattttag ttgtgactat gtatcgtatt tattgtcgtc acaagaatta tttcgaaatc
    16561 taaagtgtta tttaaatatg tgtcaattaa cattagaaga gttatacgtt ttaggaatat
    16621 taaatctgca taaaggtcaa ttgacggtta aagaattaca gggtgaattc catcacccga
    16681 tatttgcggt tagcccgatt ttaaagtgtt taattttgaa gggattagtt aaaaaagaga
    16741 gatgtgaatt agatgaaagg cgtgttattg taacaatcaa gcgagaaaag ttttctaaag
    16801 tcactatgct tattcaagca tgttataatt atcttgaaaa gggaatacaa gttaagctta
    16861 ataataaata gttaatcact tgtatttcac atacctatga agaatagttt atagttttaa
    16921 gtgggaggga tattatgatt ttagataatg tgaatccaaa tgatttattt ccaactgaaa
    16981 aaaagggtcc gtcagtgcta ggaataattg agtataaagt tcaaggtgag aatgaatttg
    17041 aaggggcatt tatagcaaca aatgaaagat taattatgaa tgtagatatg aatggccaat
    17101 tttattatag aagtattagt tataatgaaa tagagaagat agattatgat ggtcaaacta
    17161 taatgttcaa atttaatatt ggtaatgtcc caatgcatga tattaaaagt gataacgttg
    17221 aaatgtttgt tgaatatgta aaacaacata tgatagtata atgtcaatgt atgtaggttg
    17281 ataagaagag tttgaatgat aactcattgc atttaaagat aaatggcatg cgttatgatt
    17341 taaagctctt ctttttttat attttataaa ttttgtgagc aagccatcca attaatataa
    17401 aaaatatcaa atcaaacacg ttgatataga ttaaaattt
//
LOCUS       DAMZLE010000047        17364 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMZLE010000047 DAMZLE010000000
VERSION     DAMZLE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17364
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.047"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            117..2213
                     /gene="pnp"
                     /locus_tag="POB92_001364"
     CDS             117..2213
                     /gene="pnp"
                     /locus_tag="POB92_001364"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI6578872.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPVERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2449..4122
                     /locus_tag="POB92_001365"
     CDS             2449..4122
                     /locus_tag="POB92_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI6578873.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4379..6748
                     /locus_tag="POB92_001366"
     CDS             4379..6748
                     /locus_tag="POB92_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6578874.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVVLENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6753..7466
                     /locus_tag="POB92_001367"
     CDS             6753..7466
                     /locus_tag="POB92_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6578875.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7497..8762
                     /locus_tag="POB92_001368"
     CDS             7497..8762
                     /locus_tag="POB92_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6578876.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTTTARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8762..10048
                     /locus_tag="POB92_001369"
     CDS             8762..10048
                     /locus_tag="POB92_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6578877.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFVIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVNEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10048..10752
                     /locus_tag="POB92_001370"
     CDS             10048..10752
                     /locus_tag="POB92_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6578878.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLILPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10857..11684
                     /locus_tag="POB92_001371"
     CDS             10857..11684
                     /locus_tag="POB92_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDI6578879.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11703..12095
                     /locus_tag="POB92_001372"
     CDS             11703..12095
                     /locus_tag="POB92_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6578880.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12129..12707
                     /gene="pgsA"
                     /locus_tag="POB92_001373"
     CDS             12129..12707
                     /gene="pgsA"
                     /locus_tag="POB92_001373"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI6578881.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12934..14085
                     /locus_tag="POB92_001374"
     CDS             12934..14085
                     /locus_tag="POB92_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDI6578882.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            14250..15293
                     /gene="recA"
                     /locus_tag="POB92_001375"
     CDS             14250..15293
                     /gene="recA"
                     /locus_tag="POB92_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI6578883.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            15647..17206
                     /gene="rny"
                     /locus_tag="POB92_001376"
     CDS             15647..17206
                     /gene="rny"
                     /locus_tag="POB92_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDI6578884.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT         6373 a         2341 c         3357 g         5293 t
ORIGIN      
        1 aataatagat ggatatcccg ctctcttttt ttccaattca atattttata actaatatta
       61 aaatacgata ataaatgata tgatataact attagattca agagaggaga tttataatgt
      121 ctcaagaaaa gaaagttttt aaaactgaat gggcaggaag atctttaacg attgaaacag
      181 ggcaattagc taaacaagca aatggcgctg tattggttcg ttatggagat acagtcgtgt
      241 tatcgacggc aactgcatca aaagaacctc gtgatggaga tttcttccca ttaacagtga
      301 actatgaaga aaaaatgtac gctgcgggta aaattcctgg tggatttaaa aagagagaag
      361 gacgtcctgg tgacgatgca acattaactg cgcgattaat tgatagacca attagacctt
      421 tattccctaa aggatataag catgatgttc aaattatgaa catggtatta agtgcagatc
      481 ctgattgttc accacaaatg gctgcaatga ttggttcatc tatggcgctt agtgtgtcgg
      541 atattccatt ccaagggcca atcgccggtg taaatgtggg ttatattgac ggtaaatata
      601 tcattaaccc aacagtagaa gaaaaagaag tttctcgctt agaccttgaa gtagctggtc
      661 ataaagatgc agtaaacatg gtagaggcag gcgctagtga gattactgaa caagaaatgt
      721 tagaggcgat tttctttggt catgaagaga ttcaacgttt agttgatttc caacaacaaa
      781 tcgtcgacca cattcaacct gttaaacaag aatttattcc agtagagcgt gatgaagcgc
      841 tagttgaacg tgtaaaatct ttaaccgaag aaaagggact taaagaaaca gttttaacat
      901 ttgataaaca acaacgagat gaaaatcttg ataacttaaa agaagaaatc gtcaatgaat
      961 ttatcgatga agaagatcca gaaaatgaat tacttattaa agaagtttat gcaattttaa
     1021 atgaattagt gaaagaagaa gttcgacgtt taattgcaga tgaaaaaatt agaccagacg
     1081 gccgtaaacc tgatgaaatc cgtccattag attctgaagt tggtatttta cctagaacgc
     1141 atggttcagg tctatttaca cgtggtcaga ctcaagcact ttcagtttta acattaggtg
     1201 ctttaggcga ttatcaatta attgatggtt taggacctga agaagaaaaa agattcatgc
     1261 atcattacaa cttcccgaat ttttcagtag gtgaaactgg tccagtacgt gcgccaggtc
     1321 gtcgtgaaat tggacatggt gcgttaggtg aaagagcatt aaaatatatt attcctgata
     1381 ctgctgattt cccatataca attcgtattg taagtgaggt acttgaatca aatggttcat
     1441 catctcaagc gtcaatttgt ggatcaacat tagcattaat ggatgcgggc gtaccgatta
     1501 aagcaccagt tgctggtatt gctatgggcc ttgttacacg tgaagatagc tatacgattt
     1561 taactgatat ccaaggtatg gaagatgcat taggtgatat ggactttaaa gtcgctggta
     1621 ctaaagaagg tattacagca atccaaatgg atattaaaat tgacggttta acgcgtgaaa
     1681 ttatcgaaga ggctctagaa caagcgagac gtggtcgttt agaaataatg aatcatatgt
     1741 tacaaacaat tgatcaacca cgtactgaat taagtgctta cgcgccaaaa gttgtaacta
     1801 tgacaattaa accagataag attagagatg ttatcggacc tggtggtaaa aaaattaacg
     1861 aaattattga tgaaacaggc gttaaattag atattgaaca agatggtact atctttattg
     1921 gtgccgttga tcaagctatg ataaatcgcg ctcgtgaaat cattgaggaa attacacgtg
     1981 aagcggaagt aggtcaaact tatcaagcca ctgttaaacg tattgaaaaa tacggtgcgt
     2041 ttgtaggcct attcccgggt aaagatgcgt tgcttcacat ttcacaaatt tcaaaaaata
     2101 gaattgaaaa agtggaagat gtattaaaaa tcggtgacac aattgaagtt aagattactg
     2161 aaattgataa acaaggtcga gtaaatgctt cacacagagc attagaagaa taatatttaa
     2221 agtcatatga cgacaatgta tcgtcatgtg atttttttat gccatttttt acgaagtgat
     2281 ccaatttgaa tttgttgtat tgaacatttt aaaacgcttt attattttgt gtgcaactgt
     2341 taattatcct gtatgtatag tgattaatag tgtacatcaa gtgtttttta acttataatg
     2401 aatagtgagt ttatatatgg acgggtaaca aatttaggag gtaagatttt gagtttaata
     2461 aagaaaaaga ataaagatat tcgcattata ccattaggcg gtgttggcga aattgctaaa
     2521 aatatgtata tcgttgaagt agacgatgaa atgtttatgt tagatgctgg acttatgttt
     2581 ccagaagacg aaatgctagg tattgatatt gttataccag acatttcata cgtacttgaa
     2641 aataaagata aattgaaggg tatattcctt acacacggac atgagcacgc gattggtgca
     2701 gtgagttatg ttttagaaca attagatgca ccagtatatg gatctaaatt gacaatagcg
     2761 ttaattaaag aaaatatgaa agcccgtaat attgataaaa aagttcgcta ctacacagtt
     2821 aataatgatt caattatgag attcaaaaac gtgaatatta gtttctttaa tacgacacac
     2881 agtattcctg atagtctagg tgtttgtatt cacacttcat atggtgccat tgtgtataca
     2941 ggtgaattta agtttgacca aagtttacat ggacattatg caccagatat taaacgtatg
     3001 gcagagattg gtgaagaagg tgtatttgtc ttaatcagtg attctactga ggcagagaaa
     3061 cctggatata atactccgga aaatgtgatt gaacatcata tgtatgacgc ttttgcaaaa
     3121 gtgcgaggtc gcttgattgt ttcatgttat gcttcgaact ttatacgtat acagcaagtt
     3181 ttaaatattg ctagcaagct aaatcgtaaa gtgtcatttt taggaagatc acttgaaagt
     3241 tcatttaata ttgctcgtaa aatggggtat ttcgacattc ctaaagattt gctaattcct
     3301 ataacagaag ttgataatta tcctaaaaat gaagtgataa ttatagctac tggtatgcaa
     3361 ggagaacctg tagaagcctt aagtcaaatg gcgcaacata agcataaaat tatgaatatc
     3421 gaagaaggcg attctgtatt tttagcaatt acggcttctg caaatatgga agttatcatt
     3481 gcgaatacat taaatgagct tgtacgtgct ggcgcacata ttattccaaa taacaagaag
     3541 attcatgctt caagtcatgg ttgcatggaa gaattaaaaa tgatgattaa tattatgaaa
     3601 cctgaatact ttattcctgt acaaggtgaa tttaaaatgc agatagcaca tgcgaagcta
     3661 gcagctgaag caggtgttgc accagaaaag attttccttg tggaaaaagg agatgtcatt
     3721 aattacaacg gtaaagatat gatattaaat gaaaaggtaa attcaggaaa tattttaata
     3781 gatggtattg gtattgggga tgtaggaaat atcgtgttga gagaccgtca tcttttagca
     3841 gaagatggta tctttattgc tgtagtaacg ttagatccta aaaatagacg tatagctgcg
     3901 ggacctgaaa ttcaatctcg tgggtttgta tatgtacgtg aaagtgaaga cttattacgt
     3961 gaagcagaag agaaagtacg tgaaatagta gaggctggtt tacaagaaaa acgcatagaa
     4021 tggtctgaaa ttaagcaaaa tatgcgtgat caaattagta aactattatt cgaaagtaca
     4081 aaacgtcgtc ctatgattat tccagtaatt tctgaaattt aatcaaaaag tcattaacat
     4141 aaaagaggtc agaacaagtc actgaaatat aatggttgtc atggacaatt tacttatatt
     4201 ttatgatggt caattgaaag gggtaacgat taatctgtta tcttaagtaa attgatacat
     4261 agatgatatt gttctaacct ctttcatcgt ctgtttggac tacatattct aaacatcaaa
     4321 taggaaatta tatataataa cgtcgtttta actaaggcaa cataaggagg tgcgtcaatt
     4381 ggcacaagca aaaaagaaat cgacagctaa gaaaaaaaca acatcaaaaa aaagaacaaa
     4441 ttcgaggaaa aagaagaatg ataatccgat acgttatgtc atagctattt tagtagttgt
     4501 attaatggtg ttgggtgttt tccaattagg aataataggt cgtctaattg acagcttctt
     4561 taattattta tttgggtaca gtagatattt aacatatatt ttagtactct tagcaactgg
     4621 ttttattaca tactctaaac gtattcctaa aactagacga acggctggtt cgattgtatt
     4681 gcaaattgca ttgctatttg tatcacagtt agtttttcat tttaatagtg gtatcaaagc
     4741 tgaaagagaa cctgtacttt cttatgtgta tcagtcatac caacacagtc atttcccaaa
     4801 ttttggtggc ggtgtattag gcttttattt attagagtta agcgtacctt taatttcatt
     4861 atttggtgta tgtattatta ctattttatt attatgctca agtgttattt tattaacaaa
     4921 ccatcaacat cgtgaagtcg caaaagttgt actggaaaat ataaaagctt ggtttggttc
     4981 atttaatgaa aaaatgtcgg aaagaaacca agaaaaacaa ttgaagcgtg aagaaaaagc
     5041 gagacttaaa gaagaacaaa aggcacgtca aaatgaacag ccacaaataa aagatgtgag
     5101 tgattttacg gaagtgcctc aagaaagaga tattccaatt tatgggcata ctgaaaatga
     5161 aagtaaaagt cagagtcaac caagtcggaa aaagcgagtg tttgatgcag agaatagttc
     5221 gaataacatc gtaaatcatc atcaagcaga tcagcaagaa caattaacag aacaaactca
     5281 taacagtgtt gaaagtgaaa acactattga agaagctggt gaagttacga atgtatcgta
     5341 tgttgttcca ccgttaactt tacttaatca acctgcaaaa caaaaagcaa catctaaagc
     5401 tgaagtgcaa cgtaaaggac aagtactaga gaatacatta aaagattttg gggtaaatgc
     5461 aaaagtgaca caaattaaaa ttggtcctgc agtaactcaa tatgaaattc aaccagctca
     5521 aggggttaaa gtgagtaaaa ttgtaaactt gcataatgat attgcattag ctttagcagc
     5581 aaaagatgtt agaatcgaag cgccaatacc tggtcgctct gcagtaggta ttgaagtgcc
     5641 aaatgagaaa atttcattag tttcactaaa agaagtttta gatgaaaaat tcccgtctaa
     5701 taataaacta gaagttggat taggaagaga tatatcaggt gatccaatta ctgttccact
     5761 aaatgaaatg ccacacttat tggtggcagg atcgacgggt agtggtaaat ctgtttgtat
     5821 aaatggtatt attacaagta ttttattaaa tgctaagccg catgaagtta aacttatgtt
     5881 aatcgatccg aaaatggttg aactaaatgt ttataacgga attccacatt tattaattcc
     5941 ggttgttaca aatcctcata aagctgctca agctttagaa aaaattgtag ctgagatgga
     6001 aagacgttat gatttattcc aacattcatc aactagaaat attaaaggtt ataacgaatt
     6061 aatccgtaag caaaatcaag aattagatga gaagcaacca gaattacctt atatcgttgt
     6121 tattgtagat gagcttgcag atttaatgat ggtagctggt aaagaagttg aaaatgcgat
     6181 tcaacgtatt acacaaatgg cacgtgcagc aggtatacat ttaattgtag cgacacaaag
     6241 accttctgtg gatgtaatta caggtatcat taaaaataat attccatcta gaattgcttt
     6301 tgctgtaagt tctcaaacag attcaagaac tattattggt actggcggcg cagaaaagtt
     6361 acttggtaaa ggtgacatgt tatacgttgg aaatggtgat tcatcacaaa cacgtattca
     6421 aggggcgttt ttaagtgacc aagaggtgca agatgttgta aattatgtag tagaacaaca
     6481 acaggcaaat tatgtaaaag aaatggaacc agatgcacca gtggataaat cggaaatgaa
     6541 aagtgaagat gctttatatg atgaagcgta tttgtttgtt gttgaacaac aaaaggcaag
     6601 tacatcattg ttacaacgcc aatttagaat tggttataat agagcatcta ggttgatgga
     6661 tgatttagaa cgcaatcagg taatcggtcc acaaaaagga agcaagccta gacaagtttt
     6721 aatagatctt aataatgacg aggtgtaaaa aaatgtcaga aatgaatgcg gtatataacg
     6781 ttaaacaata cattttaaat ttgattaagc aaaataaatt ggaatatggt gaccaacttc
     6841 caagtaattt atcaattgcc agagaattaa atgtaaaaac cgacgatgtt tatgaagcaa
     6901 ttcaggcatt gattactgaa caagtcatta aagataattt tgaagagggc acaagtgtta
     6961 agtcactgcc ccctttcttt tatccattga atgaacttat aagtattggg caaatgatta
     7021 aaaatgcagg atttgaatgc ggaactgaat acttaaattt tgatgagcaa ccagcaacta
     7081 tgttagatgc aaatttgttg agcgttgaag aaggatatcc agtaaccatt atagaacgat
     7141 tacgaactgc cgatggagaa ccggtcgtct attgtttaga taaaattgct aaaaaagaat
     7201 taacatgtac agagtatcaa atgagcaatg gatcgatact aagtgcgata aaagaacaaa
     7261 gtaatcataa tatttgttac gctgatacag aaattgaagc ggtaaattat gaacctcgaa
     7321 tatccgaagt actgaatgct tcgccacacg aaggtttgat tttattaaaa attacgcact
     7381 acaatgaatt agatgaacct attttgtatt cattaaatta tatgaaaaat agcttagttc
     7441 aatttaaaat cactagaaaa atatagatgt aagaagatat agaggaggct atatatttga
     7501 gtagacaatc tcaaccaaat atacatatca aagtttcacc aacaactaaa tttaaaacaa
     7561 ctactatagt ttttaaattt atggcacctt tagaatatga cacaactaca gctagatcat
     7621 tattaagcaa attattagtt cgagcaacta agaaatggcc aaccgataag tcgtttaata
     7681 atcacttagc cgatttatat ggtgcatatg tgaatagtac aatttcaaaa ttcaaagatc
     7741 agcatgtcat tacattttca ttagaaattg taaatgaacg ttatttaaga aacggtgaat
     7801 cattatttaa tcaaggatta gatttattac aagaaatcat ttggaatcca ttaattgaaa
     7861 ataaagcatt caatgataat tttgttaacc aagagaaaac attattagcc aaaaaaatag
     7921 aagcaatggt agataacaaa gcacaatatt cgtttttaaa attactcgac catatgtttg
     7981 aaaatgaagc atacaaatac ttatctacag gacaactaga acaaatccca catattactg
     8041 ctgaaacact atatcataca tatcaatcaa tgattaataa tgatcaatgt tctgtttatg
     8101 ttgtcggcaa tgtagaacct gaaagtgttg agaaacaaat acgtgaaaaa tttgcactta
     8161 aaccattcga taaacatcaa ttccaacatt ctacacatca tttacacgat gaagaagttg
     8221 attatattgt tgaatatgat gacgtggatc aagctaaatt aaatatggga tatcgttttc
     8281 caacacaata tggacaaagt ggatatgctg cttttgttgt atttaacatg atgtttggag
     8341 gagatccttc atctgtttta tttaatgaag tgcgagaaaa gcaaagttta gcgtactcta
     8401 tacattcaca aattgatggc aaaaatggct atttatttgt tttgagtggg gtttcaagtg
     8461 ataagtacga aactgcaaaa gacactatta taagtgaatt tgaaaaaata aaagcaggag
     8521 atttcactga agaaaaatta gagttagcta aaaaagtaat catttctcat cgatatgaat
     8581 ctgaagatcg tccgaaaagt attatagaga ttatgcataa ccaaatatta ttagagcaac
     8641 cacaaagcaa agaaacattt ataaatgata tacagaaggt aagtcgcgaa gatattgttt
     8701 ctgttgctga aaaagcattt ttagatacaa tctatgtgtt gacaaaagga ggggataaat
     8761 aatgaaagag cgttattatg aattaataga cgaaagagta ttcgaacaag aattagaaaa
     8821 tggtttacga ttatttgtta tccctaaacc aggttttcaa aagacatttg tcacttacac
     8881 tacacaattt ggttcattag ataatcaatt caaacccctc ggacaagacc aatttgttac
     8941 tgtacctgat ggagttgctc actttttaga acataaatta tttgaaaaag aagaagaaga
     9001 cttatttact gcatttgctg aagataatgc acaagcaaat gcgtttacaa gctttgatcg
     9061 tacaagctac ttgttcagtg caactgataa tattgaaaac aacattaaac gtttacttac
     9121 aatggttgaa acgccttatt ttacaaaaga aactgttgat aaagaaaaag gtattattgc
     9181 agaagaaata aaaatgtatc aagagcaacc tggatataaa ttaatgttta atacattgcg
     9241 tgcaatgtat caacaacatc caatacgtgt tgatattgcc ggtagtgtag aaagtatata
     9301 cgatattaca aaagatgatt tgtatctatg ttatgaaacg ttttatcatc catcaaatat
     9361 ggttttattt gttgttggcg atgtggatcc tgaagcaata tgtcgaatag taaaacaaca
     9421 cgaggatgct cgtaataaag ttaaccaacc caaaatcgaa cgaggacttg ttaatgaacc
     9481 ggaggatgtt aaagaagcat ttgttactga atctatgaaa attcaatcac caagactaat
     9541 gcttggtttt aaaaataaac cattacaaga agcgcctcaa aaatatgtac aacgtgattt
     9601 agaaatgtca ttattctttg agttaatttt tggggaagaa acagattttt atcagaattt
     9661 attaaacgaa ggacttatcg atgatacatt tggttatcaa tttgtactag agccgactta
     9721 tagtttttca atcgtgacaa gcgctactga agaaccagat aaattaaaaa aattattatt
     9781 agatgagttg cgtgataaaa aaggcaattt ccaagatgca gaagcatttg aacttttgaa
     9841 aaagcaattt ataggtgaat tcatatcaag tttaaactca cctgaatata ttgctaatca
     9901 atatactaaa ttgtattttg aaggtgttag cgtgtttgac atgttagaca ttgttgaaaa
     9961 tatcacctta gatagtatta acgaaacgtc atcgttatat ttaaatttag atcagcaagt
    10021 cgatagtcgt ttggagatta aaaagtaatg aaagcattag tattaggtgg ttctggttca
    10081 attggttctg agatagtcaa acaattatta actgatggat ttgaagttta tgtgcaatat
    10141 tatcgtactg atataaatga attaactagc aaatttaatg atgataaagt tcgttttata
    10201 caagcggatt tatctcaaac aattgatatt gacaaaacat ttggtgacat taaatcatta
    10261 gactgtttaa tatatgcaag tggtcagtct ttatatggtg ttttacaaga tatgaaagac
    10321 catgatattg atgcatgtta tcagttaaat gtcttgcaat taattcgatt atgtagatat
    10381 ttcgttgatg ttttacgtca aagtgacaat ggaagaatta ttgtaatttc atcaatttgg
    10441 ggtgagacag gagctagtat ggaaactatt tattcgacga tgaaaagtgc acaattaggt
    10501 ttcgttaagg cgcttagtca agagcttgca ctaacatcag tgacagtaaa tgctatcgca
    10561 cctggatttg tagccggtaa tatggcaagt gagtggcaag aagatgaact tcaagcaatg
    10621 ataactgaat taccacaaca gcgattgatt ttaccgagtg aggttgctca tacatgcgcc
    10681 tatttatatc acccaaatgc tagaagtgtt actggaacta tacagaaagt taatggtgct
    10741 tggtatattt aaaatataga actattttta tgagcaaatg tttttatgat tgatgttata
    10801 atatgaatat tacaatgaat cataatttgt taattaaaca aggggtgagg ttcaccatga
    10861 cagttgcaga gaaaaaagaa tggtacctag aatacgaaat tgaaattaat agaccgggtc
    10921 ttttaggtga tgtatctagt ttattaggta tgttaggtat aagtattgtt acaattaatg
    10981 gtgttgatca aggtaaacga ggccttttaa ttaaaacaga caatcttgaa aaagttgaac
    11041 gttttgagca aatagctcgt ggtataaatg aaattgaaat aacaaagctt aaaaaaccag
    11101 aattaagaga ccgtcttgca gtaagacatg gtagatatat tgagcaagat gcaaaagata
    11161 agaaaacctt tcgatttgag cgtgaagatt taggcttgtt agtagacttt ttagctgaat
    11221 tgttcaaaga agaaggtcat aagttgattg gcattagagg tatgccacga gttggtaaaa
    11281 ctgaatcaat tgttgcggga agtgtttgtg cacataagag atggttattt attagttcta
    11341 ctttaataaa acaaactgta cgtagctctc taattaaagg ggaatatgat gccaatcatg
    11401 tatacattat tgatggtgca gttactgcca gagaatctaa tccaaaacat caagagcttg
    11461 ttaacgaagt tatgacgtta ccatcaatca aagtcgttga acatccagat ttatttgttg
    11521 aaacaagtac ttgtacaatg gaagattttg actatattat cgaattgaga gaaaatgaaa
    11581 atcaagaaat acattacgaa gaaatgaaga aacagacagt ccaaagtaag aataatttag
    11641 attttggaga tccgtttggt ggtggttttg gtttcttcga gtaagtgtaa ggaggctatg
    11701 aattgaaaac ggtcggtgaa gcgctaaaag gtagacgtga aaggttagga atgactttaa
    11761 cagaattaga gcaacgtact ggaattaaac gtgaaatgct agtgcatatt gaaaataatg
    11821 aattcgatca actaccgaat aaaaattaca gcgaaggatt tattagaaaa tatgcaagcg
    11881 tagtaaatat tgaacctaac caattaattc aagctcatca agatgaaatt ccatcgaacc
    11941 aagccgaatg ggacgaagta attacagttt tcaataataa taaagactta gattataaga
    12001 gtaaatcaaa agagccaata caattattag taatcatggg tattacagtt ttaataactt
    12061 tattgttatg gatcatgtta gttttaatat tttaacagaa ataaattagt gagaaatgag
    12121 gatgtataat gaatattccg aaccagatta cggtttttag agtagtgtta ataccagttt
    12181 ttatattgtt tgcgttagtt gattttggat ttggcaatgt gtcatttcta ggaggatatg
    12241 aaataagaat tgagttatta atcagtggtt ttatttttat attggcttcc cttagcgatt
    12301 ttgttgatgg ttatttagct agaaaatgga atttagttac aaatatgggg aaatttttgg
    12361 atccattagc ggataaatta ttagttgcaa gtgctttaat tgtacttgtg caactaggac
    12421 taacaaattc tgtagtagca atcattatta ttgccagaga atttgccgta actggtttac
    12481 gtttactaca aattgaacaa ggattcgtaa gtgcagctgg tcaattaggt aaaattaaaa
    12541 cagcagttac tatggtagca attacttggt tgttattagg tgatccattg gcaacattga
    12601 ttggtttgtc attaggacaa attttattat acattggcgt tatttttact atcttatctg
    12661 gtattgaata cttttataaa ggtagagatg tttttaaaca aaaataaata tttgtttata
    12721 ctagatttca ttttcatatg gaatctagtt tttttaatcc caattttaga aattagccac
    12781 gcaattgttt ataatgatat attgtaaaac aatattcgtt cattttttta gggaaaatct
    12841 gtagtagcat ctgatacatt gaatctaaaa ttgatgtgaa tttttaaatg aaatacatga
    12901 aaaaatgaat taaacgatac aagggggata taaatgtcaa ttgccattat tgctgtaggc
    12961 tcagaactat tgctaggtca aatcgctaat accaacggac aatttctatc taaagtattt
    13021 aatgaaattg gacaaaatgt attagaacat aaagttattg gagataataa aaaacgttta
    13081 gaatcaagtg tacgtcatgc gctagaaaaa tatgatactg ttattttaac aggtggctta
    13141 ggtcctacga aagatgactt aacgaagcat acagtggccc agattgttgg taaagattta
    13201 gttattgatg agccttcttt aaaatatatt gaaagctatt ttgaggaaca aggacaagaa
    13261 atgacaccta ataataaaca acaggcttta gtaattgaag gttcaactgt attaacaaat
    13321 catcatggca tggctccagg aatgatggtg aattttgaaa acaaacaaat tattttatta
    13381 ccaggtccac cgaaagaaat gcaaccaatg gtgaaaaatg aattgttgtc acattttata
    13441 aaccataatc gaattataca ttctgaacta ttaagatttg cgggaatagg tgaatctaaa
    13501 gtagaaacaa tattaataga tcttatcgat aaacagacta atcctacgat tgcgcctttg
    13561 gcgggaagtc atgaagtata tattagattg actgcaaatg ccgactcaaa agaacaagca
    13621 caatcattga ttcaacctgt taaacaagaa attcttgatc gtattggaga atattattat
    13681 ggttcagatg acacattaat tgagcaagct gtaataaaga aaattcatga accttttgta
    13741 atatatgatg gtattactaa tggtgcttta tatcatcgat tgaaagaagt ggatttaaac
    13801 gatgttctaa agggtatgat taatcacaat gaaaactttg ttgatattaa taaacctatt
    13861 gagcagcaat taaaagatgc agtgcaattt gttaataaat tgtttaatgt gtcatcagca
    13921 attattctat tagagtatga tggtgtagtc catataggct atgataataa ctttgaattt
    13981 aaaactgagc aatttaaaat gtctaaatct agaaatttat taaagaacag aagtcaaaat
    14041 tatgcgctca taagattatt aaattggctt agaacaacaa attaattgta ttatcgataa
    14101 aaatataagc acgtttgttc gtttttgcgt tttgatttca agattttata cgaacaaata
    14161 ttcgcaaaac acttgtattt tattttgaat ccttgtatag tattggtaag ataattaaaa
    14221 gatagcaatt tcaattagga ggtctcgctt tggataacga tcgtcaaaaa gctttagata
    14281 cagtaattaa aaatatggag aaatctttcg gtaaaggtgc cgtaatgaag ttgggtgaca
    14341 atataggtcg ccgagtttca actacatcaa ctggttcagt tacattagat aatgcgctag
    14401 gtgtaggtgg ctatcctaaa ggacgaatta ttgaaattta tggtcctgaa agttctggta
    14461 agacaacagt agcgcttcac gctattgctg aagtacaaag taatggcggg gtggcagcat
    14521 ttatcgatgc tgaacatgct ttagatccag aatatgctca agcattaggc gtagatatcg
    14581 ataatttata tttatcgcaa ccggatcatg gtgaacaagg tcttgaaatc gccgaagcat
    14641 ttgttagaag tggtgcagtt gatattgtag ttgtagactc agttgctgct ttaacaccta
    14701 aagctgaaat tgaaggagaa atgggagaca ctcacgttgg tttacaagct cgtttaatgt
    14761 cacaagcgtt acgtaaactt tcaggtgcta tttctaaatc aaatacaact gctattttca
    14821 tcaaccaaat tcgtgaaaaa gttggtgtta tgttcggtaa tccagagact acaccaggtg
    14881 gacgtgcatt aaaattctat agttcagtaa gactagaagt acgtcgtgca gaacagctta
    14941 aacaaggaca agaaattgta ggtaatagaa ctaaaattaa agtcgttaaa aataaagtgg
    15001 caccaccatt tagagtagct gaagttgaca ttatgtatgg acaaggtatt tctaaagagg
    15061 gtgaacttat tgatttaggt gttgaaaacg acatcgttga taaatcagga gcatggtatt
    15121 cttacaatgg cgaacgaatg ggtcaaggta aggaaaatgt taaaatgtac ttgaaagaaa
    15181 atccacaaat taaagaagaa attgatcgta aattgagaga aaaattaggt atatctgatg
    15241 gtgatgttga agaaacagaa gatgcaccaa agtcattatt tgacgaagaa tagtacacaa
    15301 atttatatct atagttaaac ttagcaaata tccttatagg attgattgaa agtgatattc
    15361 atctcataaa gctagaataa tatctaactt tatgggatac actacaaatc gagactataa
    15421 ggttttttat tttatttatt attacattat caatagtttt ataatcgagc ttcaaaactt
    15481 tagaaaatag tagaaatagc attcaatata gtgcaaaagt gcaaattgat aacttgacac
    15541 ttatctccta taaaccgtac aattaatttg tatgatttat atataatttc ataaagtcat
    15601 attgaatttc atataaagag caaaccctag aaaaggaggt gtttgtgtga atttattaag
    15661 cctcctactc attttgctgg ggatcattct aggagttgtt ggagggtatg ttgttgcccg
    15721 aaatttgttg cttcaaaagc aatcacaagc tagacaaact gccgaagata ttgtaaatca
    15781 agcacataaa gaagctgaca atatcaaaaa agagaaatta cttgaggcaa aagaagaaaa
    15841 ccaaatccta agagaacaaa ctgaagcaga actacgtgaa agacgtagtg aacttcaaag
    15901 acaagaaacc cgacttcttc aaaaagaaga aaacttagag cgtaaatctg atctattaga
    15961 taaaaaagat gagattttag agcaaaaaga atcaaaaatt gaagaaaaac aacaacaagt
    16021 agatgcaaaa gagagtagtg ttcaaacgtt aataatgaag catgaacaag aattagaacg
    16081 catctccggt ctcactcaag aagaagctat taatgagcaa cttcaaagag ttgaggaaga
    16141 actgtcacaa gatattgcag tacttgttaa agaaaaagaa aaagaagcta aagaaaaagt
    16201 tgataaaaca gcaaaagaat tattagctac agcagtacaa agattagcag cagatcacac
    16261 aagtgaatca acggtatcag tagttaactt acctaatgat gagatgaaag gtcgaatcat
    16321 tggacgagaa ggacgaaaca tccgcacact tgaaacttta actggcattg atttaattat
    16381 tgatgacaca ccagaagcgg ttatattatc tggttttgat ccaataagaa gagaaattgc
    16441 tagaacagca cttgttaact tagtatctga tggacgtatt catccaggca gaattgaaga
    16501 tatggtcgaa aaagctagaa aagaagtaga cgatattatt agagaagcag gtgaacaagc
    16561 tacatttgaa gtgaacgcac ataatatgca tcctgactta gtaaaaattg tagggcgttt
    16621 aaactatcgt acgagttacg gtcaaaatgt acttaaacat tcaattgaag ttgcgcatct
    16681 tgctagtatg ttagctgctg agctaggcga agatgagaca ttagcgaaac gagctggact
    16741 tttacatgat gttggtaaag caattgatca tgaagtagaa ggtagtcatg ttgaaatcgg
    16801 tgtagaatta gcgaaaaaat atggtgaaaa tgaaacagtt attaatgcaa tccattctca
    16861 tcatggtgat gttgaaccta catctattat atctatcctt gttgctgctg cagatgcatt
    16921 gtctgcggct cgtccaggtg caagaaaaga aacattagag aattatattc gtcgattaga
    16981 acgtttagaa acgttatcag aaagttatga tggtgtagaa aaagcatttg cgattcaggc
    17041 aggtagagaa atccgagtga ttgtatctcc tgaagaaatt gatgatttaa aatcttatcg
    17101 attggctaga gatattaaaa atcagattga agatgaatta caatatcctg gtcatatcaa
    17161 ggtgacagtt gttcgagaga ctagagcagt agaatatgcg aaataatttt tgtctccctc
    17221 acaaattagt gagggagctt ttttaagttg tagtcttaat ctagttagac agcactttat
    17281 cggtaataac tatattaaac agtagttatt tgaaagtaag acggacctta tattaaataa
    17341 gaagttattg cttttaataa aaat
//
LOCUS       DAMZLE010000048        17273 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMZLE010000048 DAMZLE010000000
VERSION     DAMZLE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.048"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            79..1227
                     /locus_tag="POB92_001377"
     CDS             79..1227
                     /locus_tag="POB92_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6578885.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTVLNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            1227..2450
                     /gene="thiI"
                     /locus_tag="POB92_001378"
     CDS             1227..2450
                     /gene="thiI"
                     /locus_tag="POB92_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI6578886.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAILKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            2494..3264
                     /locus_tag="POB92_001379"
     CDS             2494..3264
                     /locus_tag="POB92_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDI6578887.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMATSMIAGSY
                     TGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            3362..3856
                     /gene="tpx"
                     /locus_tag="POB92_001380"
     CDS             3362..3856
                     /gene="tpx"
                     /locus_tag="POB92_001380"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDI6578888.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            3979..4926
                     /locus_tag="POB92_001381"
     CDS             3979..4926
                     /locus_tag="POB92_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6578889.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQLQGFMTELNQWMDTNRPKK"
     gene            5014..6216
                     /locus_tag="POB92_001382"
     CDS             5014..6216
                     /locus_tag="POB92_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI6578890.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(6462..6962)
                     /locus_tag="POB92_001383"
     CDS             complement(6462..6962)
                     /locus_tag="POB92_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6578891.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            7103..8221
                     /gene="ald"
                     /locus_tag="POB92_001384"
     CDS             7103..8221
                     /gene="ald"
                     /locus_tag="POB92_001384"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6578892.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            8345..8443
                     /locus_tag="POB92_001385"
     CDS             8345..8443
                     /locus_tag="POB92_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578893.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(8537..9592)
                     /locus_tag="POB92_001386"
     CDS             complement(8537..9592)
                     /locus_tag="POB92_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI6578894.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            9767..10456
                     /locus_tag="POB92_001387"
     CDS             9767..10456
                     /locus_tag="POB92_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6578895.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(10761..11174)
                     /locus_tag="POB92_001388"
     CDS             complement(10761..11174)
                     /locus_tag="POB92_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6578896.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            11379..12677
                     /locus_tag="POB92_001389"
     CDS             11379..12677
                     /locus_tag="POB92_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI6578897.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            13131..14072
                     /gene="pde2"
                     /locus_tag="POB92_001390"
     CDS             13131..14072
                     /gene="pde2"
                     /locus_tag="POB92_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDI6578898.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            14093..>17273
                     /locus_tag="POB92_001391"
     CDS             14093..>17273
                     /locus_tag="POB92_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6578899.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPS"
BASE COUNT         5958 a         2468 c         3206 g         5641 t
ORIGIN      
        1 taacctttat tttttgtcgg ataatagtat aatgcttgag taaaacaatt ttaacgattg
       61 atgattaatg aaagagaagt gtttattttg atatatctag ataatgcggc aacgacgaaa
      121 gcatttgaag aagtgttaga tacttattta aaagtaaatc aatcaatgta ttataatccg
      181 aatagtccgc ataaagctgg tttgcaggca aatcaattac tacaacaagc aaaagcccaa
      241 attaatgcaa tgattaattc aaaaacaaat tatgatgttg tattcactag tggtgcaact
      301 gaatccaata atcttgcttt aaaaggtatt gcctatcgta aatttgatac agcgaaggaa
      361 ataattacat ccgtgttaga gcatccgtcc gtattagagg ttgtaagata tttggaagca
      421 cacgaaggat ttaaagttaa atatgttgat gtaaagaaag atggcagtat taacttagaa
      481 cacttcaaag aattaatgtc agacaaagtc ggtttagtaa catgtatgta tgtaaataat
      541 gtaactggac aaatacagcc tattccacaa atggctaaag ttataaaaaa ttatcctaag
      601 gcacattttc atgtagatgc ggttcaagca ttcggcaaaa tttcaatgga tctcaataac
      661 atagatagta ttagtttaag tggacacaag tttaatggtt taaaaggcca aggcgtctta
      721 cttgtaaatc acattcaaaa tgttgaacca actgtccatg gtggtggtca agaatatggc
      781 gttagaagtg gaacagttaa tttgccaaat gatattgcaa tggttaaagc gatgaagata
      841 gctaatgaaa actttgaagc attgaatgca tttgttactg tgttaaataa tgacgtccgt
      901 caatttttaa ataaatatca tggagtttat attaattctt caacttctgg ttcaccattc
      961 gttttaaata ttagttttcc tggcgtaaaa ggtgaagtat tagttaatgc tttttcaaaa
     1021 tatgacatta tgatatctac gacgagtgct tgttcatcta aacgtaataa attaaatgaa
     1081 gtattggctg caatgggatt atcagacaaa tctattgaag gtagtataag attatcattt
     1141 ggggctacta caactaaaga agatatagca aggtttaaag aaatatttat catcatttat
     1201 gaggaaatta aggagttgct aaaataatga agtatgatca cttgcttgtt agatacgggg
     1261 agttaacatt aaagggttca aatagaaaaa aatttgtaaa tcaattaaga aataatgtaa
     1321 ataagtcatt aaaaggactt gatgggtttg tcgttaaagg caaacgagat cgtatgtata
     1381 ttgaacttga agaccatgca gatataaatg aaataacata tcgattatca aaaattttcg
     1441 gtattaaatc tattagtcca gtattaaaag tagaaaaaac aatagaggca atgagtgcag
     1501 cggcaattaa atttgcgcag caatttgaag aaaacagcac atttaaaatt gatgtgaagc
     1561 gtgccgataa aaatttccca atggatacgt atgaattaca gcgtgaattg ggtggtgcaa
     1621 tattgaagca cttcgacaat atttcagtga atgtcaaacg tccagatcat gaaattcgag
     1681 tggaagttag attagatgca atttatatgt atgaagaagt tgttccgggt tcaggtggat
     1741 taccagttgg tactggtggt aagacgttac taatgctttc aggcggtata gactcaccag
     1801 ttgctgggat ggaagtgatg agacgtggcg taacaattga agcgattcat ttccatagtc
     1861 caccatttac aagtgatcaa gcaaaagaaa aagttattga attgacacgt attttagctg
     1921 aacgtgttgg accaattaaa ttgcatattg taccatttac agaattgcaa aaacaggtaa
     1981 ataaagttgt acatccaaga tatacaatga cttcaacgag acgtatgatg atgcgtgttg
     2041 ctgataaatt agtacatcaa ataggggctt tagctattgt aaatggtgaa aacctagggc
     2101 aggtagccag tcaaacactt catagcatgt atgcaattaa taatgtaact tctactcctg
     2161 tattacgtcc tttattaact tacgataaag aagaaattat tattaaatcg aaagaaattg
     2221 gtacatttga aacatctatt caaccatttg aagattgttg tacaattttc acccctaaaa
     2281 atccagtaac cgaaccaaac tttgataagg tagttcaata tgaaagcgtc tttgattttg
     2341 aagagatgat taatcgtgct gttgaaaata ttgaaacact tgaaataact agtgattata
     2401 aaactattaa agaacagcaa acaaaccaat taataaatga ctttttataa ataaaatcct
     2461 agagtaaatt taaacataag gggatgttaa actatggatt tgaacttaac gatgattata
     2521 atcataattt tatttggttt tatcgcggcg tttatagatt cggttgtagg gggtggcggt
     2581 ttaatttcta cgccagcatt attagcaatc ggtctaccac catctgtggc tttaggtaca
     2641 aataaattgg caagttcatt tggttcttta actagtacga taaagtttat aaggtccggt
     2701 aaagtggact tatatgttgt tgccaaatta tttggttttg tatttttggc atctgcatgt
     2761 ggcgcatata ttgcaacgat ggttccgtca caaatattga aacctttaat cattattgca
     2821 ctttcgtcgg tgtttatatt cacattactt aaaaaagatt ggggcaatac acgtacgttt
     2881 actcaattta catttaagaa agccatacta tttgcagcac tttttatatt aatcggcttt
     2941 tatgatggat ttgtaggtgg gggaacaggt tcatttatgc tttttgtatt gttagtcttt
     3001 ggttttgatt ttttaagtgc agcaggaaat gctaaagttt tgaactttgc ttctaatata
     3061 ggtgcgcttg tattatttat ggtattagga caagtagatt atgtaatagg tttaataatg
     3121 gctacaagca tgattgctgg gtcatatact ggggcacatt ttgctatcaa acaaggtgtt
     3181 ggctatgtaa aagtattatt tattattatt actgctattt taattttaaa aaatgctttt
     3241 gattatattc aacaatttgt ataaaattaa atttgctttt gattaaagca tgaaaattgt
     3301 aatcaaacca taaattgtcg tatgatgtag ttagaatttt aaaatgcagg aggtcaagta
     3361 tatgactgaa ataacattca aaggtggacc aatccactta aaaggtcaac aaattaatga
     3421 aggtgatttt gcacctgatt ttacagtgtt agataatgac ttaaatcaag taacattagc
     3481 agattatgct ggtaaaaaga aattaattag tgtggtacca tcaattgata caggtgtttg
     3541 tgatcagcag actcgcaaat tcaactctga tgcttctaaa gaagagggga ttgtgcttac
     3601 aatttcagca gacttaccat tcgcacaaaa aagatggtgc gcttcagcag gtttagacaa
     3661 tgtcattaca ttaagtgacc accgtgactt atcatttggt gaaaactatg gcgttgttat
     3721 ggaagaactt cgcttattag ctcgtgcagt atttgtatta gatgcagata ataaagttgt
     3781 ttataaagaa atcgttagtg aaggtactga tttcccagat tttgatgctg ctttagctgc
     3841 atacaaaaat atttaatcat taaagagata aatcttaaaa tgtatacatc gtgtccatcg
     3901 ttgtcaacag cattaaaata gaattgtttt ctatgattgc taagacctat gggcactttt
     3961 tattggagag ggacgaatat ggcagaacaa caaacaatta tggaacgctt gtttcataca
     4021 ttagatgaaa aagctaaaac attaaataat gaaaatggcc aaagttttat tgaaaatctt
     4081 gggctagcaa tggaacaagt atataccaat gaaagaggat tgttagaaca atcgacgtta
     4141 caagatcgac gtaaagcatt ccaatttgca tatttaagtt taatgcagga agaaaagata
     4201 caagcaaatc atcaaattac accagattca attggattga tactaggatt tttagttgag
     4261 cgttttatga acaaccaaga agaattacat attgttgata ttgcaagtgg tgccggtcat
     4321 ttaagtgcta ctgtaaaaga agtgttacct gaaattgcgg ttatgcatca tttaattgaa
     4381 gttgatccag ttttatcacg tgttagtgta catttagcaa acttcttaga aattcctttc
     4441 gatgtgtatc ctcaagatgc catcatgcca ctaccattag aagaagcaga tatcgttatt
     4501 ggtgattttc cagtaggcta ttatccaatt gatgaaagaa gtaaggagtt taagctaggt
     4561 tttgaagaag gacatagtta ttcacattat ttattaatag aacaagcaat aaatgcatta
     4621 aaagatgctg gatatgcctt tctagtggta ccaagtaata tttttacagg tgaacatgta
     4681 aaacagcttg aaaaatatat tgcaacagag acagagatgc aagcattttt aaatttacca
     4741 ccaactttat ttaaaaatga aaaagcgcga aaatctatat taattttaca aaagaaaaaa
     4801 tcgggtgaaa caaagccagt tgaagtatta ttggcaaata ttcctgattt caaaattcct
     4861 tcacaattac aaggatttat gacagagtta aatcagtgga tggacacaaa tcgtcctaaa
     4921 aaataatctg taatagtata gtcataaaac tgtatgaatg ataaaatgaa aaatgaaata
     4981 gtataaaaag caaacgatgg aggatcatta cttatgtcaa aattaatctt ggctatcaat
     5041 gctggtagtt catcattaaa atttcaatta attagaatgc ctgaagagga attagtaaca
     5101 aaaggtttag tagaaagaat cggattgaag gattcaattt ttacaattga agtcaacggg
     5161 gaaaaagtta aaacagtaca agatatcaag gatcacgttg aggcagttga tatcatgttg
     5221 gatgctttta aagcgcacaa tattattaat gatattaacg atatcgatgg aacaggtcac
     5281 cgtgttgtac atggtggtga aaaattccct gaatcagtag ctattactga tgaggttgaa
     5341 aaggaaattg aagaattaag cgaattagca ccgcttcaca acccagctaa cttgatgggt
     5401 attcgtgctt tccgtaaatt attaccaaat atccctcatg tggcaatttt tgatacagca
     5461 ttccatcaaa caatgcctga aaaagcatat ctatatagct tgccatatca ttattataaa
     5521 gattatggca ttcgtaagta tggtttccat ggtacaagcc ataaatttgt atcacaaaga
     5581 gcggcagaaa tgttagataa gccaattgaa gatttacgta ttatttcatg tcacattggt
     5641 aatggagcat caattgcagc tatcgatggc ggtaaatcta ttgatacatc aatgggcttc
     5701 acaccacttg ctggtgtaac aatgggtact cgttctggta atattgaccc tgctttaatt
     5761 ccatttatta tggaaaaaac aggtaaaact gctgaacaag tattggaaat cttaaacaaa
     5821 gaatcaggtt tattaggatt atcaggtaca tctagtgatt tacgtgactt atcagaagaa
     5881 gctgaatcag gtaaagcacg ttcacaaatg gctttagatg tatttgcttc taaaattcac
     5941 aaatatattg gatcatatgc tgcgagaatg catggtgttg atgttatcgt atttacagca
     6001 ggtatcggtg aaaactcagt agaaattcgt gcgaaagttc ttgaaggctt agaatttatg
     6061 ggtgtttact gggatcctaa gaaaaatgaa aacttactac gtggtaaaga aggatttatt
     6121 aactatcctc attcaccagt taaagttgtc gttattccaa cagatgaaga aagtatgatt
     6181 gcccgtgatg ttatgacatt cggtggttta aaataatttt taaataaata ttaagcgtct
     6241 atctccgcta caattatatg taggaggtag acgctttttg tattgtttga aaattaacta
     6301 gtgtaatgtc tcttttcacg aatatcttaa agaattaagt agtcgaagaa gattatgaaa
     6361 acattattaa gtgagtttta tttataaaca ataaaaaacc aagtgcagaa agttgcactt
     6421 ggttcataat gatcaagtag ttatctaatg atcatctata tttaattttg atatttttca
     6481 cgtaattgag ttgttgcaac ttgtggttgg aagtctgctg gtaactcttc agtacgaaca
     6541 actaacacgt cacatggcgc atgacgaacg atagattctg atacagaacc aacaataaat
     6601 ctttccacgg catttaagcc tgatgtacca ctcataatca agtctgcatt aatttcatgt
     6661 gcaagctttt taggaatgat agatttagga gagccaaact ctagacgcgt ttctacatct
     6721 ttaacaccag cgttagtagc tacttcttta taaccattta ataattcttc tgcaaaatgc
     6781 ttagattttt cagtaaattg agcatcataa acttcataag aagaatacgt tcttgaatca
     6841 attacattca caattgttaa cttcgcatcg ttacgtttag caacaccaac tgctctgtta
     6901 aatgcccatt ccgcttcatg tgaaccgtca actgcgatta aaatattttt gtaagtaatc
     6961 atagtaagtg cctccttgtt ctttcttaat tatattttac cacaattttc ggaaaagtca
     7021 gtgtaataac ctttacaatt ttgtggattt ttgattgcgc tttcaaaatg ttcgcgttaa
     7081 gatgattatg gaggtggatg gtatgaaaat tggtatacca agggagatta aaaataatga
     7141 aaatcgtgtt ggtttatcac caagtggtgt gcacgcttta gtagaaagtg ggcatactgt
     7201 tttagtggaa acaaatgcgg gttcaggatc attctttgaa gatgtagatt acaaagaagc
     7261 aggtgctgag attgttgctg aacaagcaaa agtttgggat gtggatatgg ttattaaagt
     7321 taaagaacca cttgaatctg aatatccata ttttaaagaa gggcttgtat tattcactta
     7381 tcttcattta gcaaatgaag aaaaattaac acaagctttg atagatagaa aagtaattag
     7441 tattgcatat gagactgtgc agttaccaga ccgatcttta ccattgttat caccaatgag
     7501 tgaggtagca ggaagaatgt cagctcaagt tggcgcagag ttcctacaaa aacttaatgg
     7561 tggtatggga attctactag gtggtgtccc aggagtacct aagggtaaag taactattat
     7621 cggtggtggt caagcaggaa caaatgcagc taaaattgca ctaggactag gtgcagatgt
     7681 tacgatttta gatgttaatc caaagcgttt acaacaatta gatgatttat tcggtggacg
     7741 tgtacataca attatgtcaa atccgttgaa tattgagttg tatgttaaac aaagtgattt
     7801 agtaataggt gcagttttaa ttccaggtgc taaagcgcca agacttgtaa cagaagacat
     7861 gattaaacaa atgaaaaatg ggtcagttat tattgacatt gctattgatc aaggcggtat
     7921 ttttgaaaca actgataaaa ttacgacaca tgatgatcct acatatatta agcatggtgt
     7981 ggttcattat gcagttgcaa atatgccagg tgcagtaccg cgtacttcga cgttagcttt
     8041 aaataatgct acgctacctt atgcgctcat gctagctaat aaagggtata gagaagcatt
     8101 taaatcaaat caaccattat cattaggttt aaatacttac aaaggtcacg taaccaataa
     8161 aggcgttgca gaggcatttg aaatggaata taaatctgta gaagaagcat tacaattata
     8221 atttgaattt gatgaaaagg gaatggatta cagcgtacta aagaatgacg ttatcatctc
     8281 cttggttatt aaaattaaat aatgataaat taaagctaga aacaatgcga agaaatcatc
     8341 gaacatgaat gatattgatt tgattcatta catgttactg atgaacatac ctgcatatgt
     8401 gtttctagct tgtttgttac atattgcatt tagacttgga taaaaattta tgtcttaggc
     8461 gctacaatgt tatattggca gtgtttgact gaatgagaat gcgcttgtaa caagcttttc
     8521 atttttatcc cactccttat ttttcgtaat gtgttaatac ttcatatcct tcattagtga
     8581 caagtatgtc atcttcaatt cttacacctg caacaccagg tacataaata cctggttcga
     8641 ttgtaataac catgccagct tctaacaaat tagaattagt acttgaaaca tcttgatatt
     8701 catgttcttg taatcctagg ccatgaccta agcgatgagg gaaatattca ccataacctt
     8761 tttctgaaat aatatttcta gcgatatgat cgatatcttt taatggtatt ccaggtttaa
     8821 ttgcttggat tgcagatgtt tctgcttcta atacaatatt ataaatttct tgtgcttctt
     8881 tgctaggttc accaaattta atagtacgtg tcatatcgct acaataatgc tcataaatta
     8941 cacctaaatc aaatagtaca tattcattgc tttttaatct gcgatctcct ggtgtgccat
     9001 gaggtgatgc ggcatgatct ccaaataaaa ccatcgtatc aaaactcatt tcattgacgc
     9061 catattgttt gatagtttgc tcaatatggt tgactacttc acgttcagtc acaccttctt
     9121 ttaaataaga aacacctatt tcgatacact tatctgctaa ctcagcagct ttacgtattt
     9181 tgctaatttc atcttcggat ttaatatttc tcaattgttt gattgttaaa tcaacatctc
     9241 cgaatgaatt gacattgaaa ccagagatta attgtttttg gcgtgctact gttaagtgct
     9301 cgctttcaat tagtaattta ttgattgttt gaggataaag tgaaaaaggg ttttcagtgt
     9361 ctaaatatcc aacgatttca cctgtgaaag gtgatgcttt gacttcttcg acttccattt
     9421 ttggacaaaa tagtacttgt ttaccatctt tcttaatcaa taatgcaaat aatctttcat
     9481 ggggttcgct acggtatcca gtaaaataat atacattcaa cggtgttgta atccatgctg
     9541 catcagcttg ttgattgttc aattcgtcta ttatttttga tatttttgtc attttataaa
     9601 cactcctcaa cataatatta cttccatttt atgccaatta ttattagatt tcaaaatcga
     9661 gctaatacat taactagatt ttcaattttt aaaatttgag aatttgttat aattaagtgg
     9721 caaaagggta ctaaatttat atagtaattg ataggaggat gctcaaatga agttatcatt
     9781 tcatggtcaa tcaacgattt atttagaagg gaacaacaaa aaggttatcg tagatccgtt
     9841 tatttcaaat aaccctaagt gtgatttgaa tattgaaaca gtgcaggtag actatatcgt
     9901 tttaacgcac ggtcattttg atcattttgg tgatgttgtg gaactagcaa agaaaacagg
     9961 agcaacagta attggaagtg cagaaatggc tgactatctt tcttcatatc acggtgttga
    10021 aaatgttcat ggtatgaata ttggtggtaa agcaaacttt gatttcggaa gcgttaaatt
    10081 tgttcaagca tttcatagtt caagttttac acatgaaaat ggtattccag tttatttagg
    10141 tatgccaatg ggtatcgtgt ttgaagttga aggaaaaaca atttatcata ctggtgatac
    10201 aggtctgttt agtgacatgt cacttattgc gaaacgtcat ccagttgatg tatgttttgt
    10261 accaattgga gataatttta cgatgggtat tgatgatgca agttatgcta ttaatgaatt
    10321 tattaaacca aaaatttctg taccgattca ttatgataca ttcccattaa tcgaacaaga
    10381 tccacaacaa tttaaagatg ctgttaatgt aggggacgtg caaattttaa aaccaggtga
    10441 atctgttcag ttttaaatag aatatagatt ttacactaaa aaacacctcg ctaatctatg
    10501 tggaagttat cgtctgcata tttttagtgg gtgtttttat attctactga ttcagttacg
    10561 acgtggacgg gggacatcag tggttcttta tcatttctga cctactctcg taattttagt
    10621 tttatacaat tagacattga ttataaattt actacgatat aaaaaatcca gaaccacagt
    10681 cataatcgtt tcttgtattt actcagtcgt ttgagactgc atcatgattg aaagattatt
    10741 tgtgattctg gataaaaatt ttatttaacg attaatacag ggatagtcgc acgttttgct
    10801 actttgtggc tgacacttcc aagtacaaat ttctttttgt cttctgcttt acggttactt
    10861 aaaacaacaa tttcgtattt accgctatta gcatgtttta atagttcttc ttttgcatta
    10921 ccacgaacaa ttatttggtc ataatcaata ccataatctt ctaaagcttt gcgtgttttt
    10981 tccaatcgct tgcttcgttc ttcagtaagt ttgtttaaat gaacacctgc tttaattgat
    11041 gcttgagcat cttgttcgct gattgcgttt aaaactgtta cgactgtacc ttcgccagct
    11101 aatttagaca cttcttttag tgctttttca ttttttaact gagtgtctac accaagtaat
    11161 atatttttat acattagtac atccctcctc acttacattc taataaataa taatggattt
    11221 ttccatttaa aattaaagat tttgtgatct ttgtgtttgt tgtatatttt caaaaaataa
    11281 aataggtgaa acgatcatta tttcatttca cttcaatgct ttttatccaa aataaagtta
    11341 taatgtagtt aatactgttc gaaaagaaga ggtaaagaat gacaaaacat gaacaaattt
    11401 tagattatat tgaatcgtta tctataggct ctaaaatttc ggtgcgtaaa atcgctaaat
    11461 ttttaaacgt atctgaaggg acagcatatc gtgcaattaa agatgctgat aaaatgggca
    11521 tggtcgcaac gatagatcgt gttggtacgg ttcgaatcga aaaacgtaat cgtaatgaaa
    11581 ttgaacattt aacttttaat gaaattgtca atattattga tggccaagtg ttaggtggca
    11641 ataaaggtat cactaagatg gtatctaaat ttgcaattgg agcaatggaa ctcaaagata
    11701 ttttaaaata tattggtcca aagacattat tgattgtcgg aaatcgtgaa gatgtgcaga
    11761 ttgaagcttt aaaaagaggt acggctatat taattactgg tggatttaag ccatctaata
    11821 aagttattaa ttttgcgaat gaacatgatt tacctgttct atcatcaagt tatgatacat
    11881 ttctagttgc aaatattatc aacaaagcac tttttaatca gaaaatacga aaagatatct
    11941 taatagttca agatataatg acgccattag atgatttatc tgtgctgttt gatacgatga
    12001 aaatagcaga ttataagcgt atggcgaatc gaactggcca tacaagattt ccggtagtca
    12061 atgagtctta taaattagta ggtattgtaa caagtcgaga gatgattaat acaaaggacg
    12121 acgacgaaat agataaagtg atgacgcgaa atcctattta tgtaaatgct atgagtacag
    12181 tagcaagttg tgcgcatatg atgatttggg aaggtattga attgattcct gtcgtttcga
    12241 gtaataaaaa gacagtgggt gtgattaata gacaagatgt tttaaagtct atgcaacttt
    12301 taggaagaca gcctcagatg ggtgaaacaa ttaacgatca aatcgccaaa tatataacaa
    12361 tgaatcaaga tggaattaca gtagaagttt caccactttt aatcaatcat tacggaacag
    12421 taagtaaagc ggcatttgta agtattatag aagaaacaat acaatatgaa atgcgtaaat
    12481 ttaaaaaagg taatgtcatg atagagaact taaatatcgt gtatattaaa acagtaccta
    12541 ttgaatcaca tataacagtg cgctttggca tactagacgt cggaagaaac ttcgccaaaa
    12601 tagaagtgaa catgcatagt caaaacgaca aagtagcgag cgctctcgtg atatgccaaa
    12661 tgtttgacga agtataggaa cttagcagag aaaataggca taagatagat ttcgcacaaa
    12721 cgttggggcc caacttgaac attatagtaa gctgacatta cgtcagcttc tatgttgggg
    12781 cccaccccaa cttgcatggt ctgtagaatt tcttttcgaa attctctatg ttggggcccc
    12841 accccaactt gcattgtttg tagaaattgg gggtccaatt tctctatgtt gtggcccctg
    12901 aattaaataa agcttgttat ttgtgcattt ttatttggtc aactgttgct agtataatat
    12961 tgcttgatgt cctataatcg ttatgaacta aatagtatga aaatatatat atctaaataa
    13021 ttaaaacggg caaatagttt aatatggtag taacaatttt tataatttag atattttgat
    13081 atagactaat gcttaaatta agcaactttt ctaaaaaagg gagtatgaaa atgattagta
    13141 ctttgaatga aattatgaaa tgtatcgaag acaacgatac aattattata cacagacatg
    13201 ttagaccaga tcctgatgca tatggttcac aacttggttt aaaatactat attcagcaga
    13261 aatttccgca aaagcaagta tttgcagtgg gtgaagcgga atcatcatta agttttattg
    13321 gagagttaga taacattgat gataaaacat atcaagatgc gcttgtaatt gtatgtgata
    13381 ctgccaatgc tccacgaatt gacgatgaac gttatagtac aggtcgtaaa cttattaaaa
    13441 tagatcatca tcctgcagtt gatcagtatg gtgatattaa tttagttaat acgaacgcgt
    13501 catctacaag tgaaatcatt tacgatttaa tctcacattt taatgatgaa gcaattgtta
    13561 ataaagatat cgcgagcgtt ttatatcttg gtatcgtcgg tgatactggg cgattccttt
    13621 ttaacaatac ctcagaacat actatggaaa ttgctggaaa gttaattggg catgatattg
    13681 atcataatgc gcttttaaat aaaatgatgg agaaggaccc aaaaatgttg ccgtttcaag
    13741 gttatgtttt acaacatttc gaacttatgg atgatggatt ctgccaagtt aaaataactg
    13801 aagatgtatt ggagcaattc ggtattcagc caaatgaagc atctcagttt gttaatacaa
    13861 ttgctgacat caaaggcttg aaaatatggg tatttgcagt cgatgaaggt aatgaaatca
    13921 gatgtcgatt acgttctaaa gggcaattga ttattaatga tattgcgcaa gattttggtg
    13981 gcggtggtca tccgaatgcg tcaggagttt cagtggacag ctgggatgaa tttgagcaac
    14041 ttgctacagc tttacgcaca aaacttaact aatagaaagg ggccattcaa tcatggtggc
    14101 atatttaaat attcatacgg cttatgattt gttaaattca agcttaaaaa tagaagatgc
    14161 cgtaagactt gctgtgtctg aaaatgttga tgcacttgcc ataactgaca ccaatgtatt
    14221 gtatggtttt cctaaatttt atgatgcatg tatagcaaat aacattaaac cgatttttgg
    14281 tatgacaata tatgtgacaa atggattaaa tacagtcgaa acagttgttc tagctaaaaa
    14341 taatgatgga ttaaaagatt tgtatcaact atcatcggaa ataaaaatga atgcattaga
    14401 acatgtgtcg tttgaattat taaaacgatt ttctaacaat atgattatca tttttaaaaa
    14461 agtcggtgat caacatcgtg atattgtaca agtgtttgaa acccataatg acacatatat
    14521 ggaccacctt agtatttcga ttcaaggtag aaaacatgtt tggattcaaa atgtttgtta
    14581 ccaaacacgt caagatgccg atacgatttc tgcattagca gctattagag acaatacaaa
    14641 attagactta attcatgatc aagaagattt tggtgcacat tttttaactg aaaaggaaat
    14701 taatcaatta gatattaacc aagaatattt aacgcaggtt gatgttatag ctcaaaagtg
    14761 tgatgcagaa ttaaaatatc atcaatctct acttcctcaa tatgagacac ctaatgatga
    14821 atcagctaaa aaatatttgt ggcgtgtctt agttacacaa ttgaaaaaat tagaacttaa
    14881 ttatgacgtc tatttagagc gattgaaata tgagtataaa gttattacta atatgggttt
    14941 tgaagattat ttcttaatag taagtgattt aatccattat gcgaaaacga atgatgtgat
    15001 ggtaggtcct ggtcgtggtt cttcagctgg ctcactggtc agttatttat tgggaattac
    15061 aacgattgat cctattaaat tcaatctatt atttgaacgt tttttaaacc cagaacgtgt
    15121 aacaatgcct gatattgata ttgactttga agatacacgc cgagaaaggg tcattcagta
    15181 cgtccaagaa aaatatggcg agctacatgt atctggaatt gtgactttcg gtcatctgct
    15241 tgcaagagca gttgctagag atgttggaag aattatgggg tttgatgaag ttacattaaa
    15301 tgaaatttca agtttaatcc cacataaatt aggaattaca cttgatgaag catatcaaat
    15361 tgacgatttt aaaaagtttg tacatcgaaa ccatcgacat gaacgctggt tcagtatttg
    15421 taaaaagtta gaaggtttac caagacatac atctacacat gcggcaggaa ttattattaa
    15481 tgaccatcca ttatatgaat atgccccttt aacgaaaggg gatacaggat tattaacgca
    15541 atggacaatg actgaagccg aacgtattgg gttattaaaa atagattttc taggtttgag
    15601 aaacttatcg attattcatc aaatcttaac acaagtcaaa aaagatttag gtattaatat
    15661 tgatatcgaa aagattccgt tcgatgatca aaaagtgttt gaattgttgt cgcaaggaga
    15721 tacgactggc atattccaat tagagtctga cggtgtaaga agtgtattaa aaaaattaaa
    15781 gccggaacac tttgaagata ttgtggctgt aacttctttg tatagaccag gtccaatgga
    15841 agaaattcca acttacatta caagaagaca tgatccaagc aaagttcaat atttacatcc
    15901 gcatttagaa ccgatattaa aaaatactta cggtgttatt atttatcaag agcaaattat
    15961 gcaaatagcg agcacatttg caaacttcag ttatggtgaa gcggatattt taagaagagc
    16021 aatgagtaaa aaaaatagag ctgttcttga aagtgagcgt caacatttta tagaaggtgc
    16081 aaagcaaaat ggttatcacg aagacattag taagcaaata tttgatttga ttctgaaatt
    16141 tgcagattat ggttttccta gagcacatgc tgtcagctat tctaaaattg catacattat
    16201 gagcttttta aaagtccatt atccaaatta tttttacgca aatattttaa gtaatgttat
    16261 tggaagtgag aagaaaactg ctcaaatgat agaagaagca aaaaaacaag gtatcactat
    16321 attgccaccg aacattaacg aaagtcattg gttttataaa ccttcccaag aaggcattta
    16381 tttatcaatt ggtacaatta aaggtgttgg ttatcaaagt gtgaaagtga ttgttgatga
    16441 acgttatcag aacggcaaat ttaaagattt ctttgatttt gctagacgta taccgaagag
    16501 agtcaaaacg agaaagttac ttgaagcact gattttagtg ggagcgtttg atgcttttgg
    16561 taaaacacgt tcaacgttgt tgcaagctat tgatcaagtg ttggatggcg atttaaacat
    16621 tgaacaagat ggttttttat ttgatatttt aacgccaaaa cagatgtatg aagataaaga
    16681 agaattgcct gatgcactta ttagtcagta cgaaaaagaa tatttaggat tttatgtttc
    16741 gcaacaccca gtagataaaa agtttgttgc caaacaatat ttaacgatat ttaaattgag
    16801 taacgcgcag aataataaac ctatattagt acagtttgat aaagttaaac aaattcgaac
    16861 taaaaatggt caaaatatgg cattcgtcac attaaatgat ggcattgaaa ctttagatgg
    16921 tgtgattttc cctaatcagt ttaaaaagta cgaagagttg ttatcacata atgacttgtt
    16981 tatagttagc gggaaatttg accatagaaa gcaacaacgt caactaatta taaatgagat
    17041 tcagacatta gccacttttg aagaacaaaa attagcattt gccaaacaaa ttataattag
    17101 aaataaatca caaatagata tgtttgaaga gatgattaaa gctacgaaag agaatgctaa
    17161 tgatgttgtg ttatcctttt atgatgaaac gattaaacaa atgactactt taggctatat
    17221 taatcaaaaa gatagtatgt ttaataattt tatacaatcc tttaacccta gtg
//
LOCUS       DAMZLE010000049        17204 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMZLE010000049 DAMZLE010000000
VERSION     DAMZLE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.049"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(187..1185)
                     /gene="sirC"
                     /locus_tag="POB92_001392"
     CDS             complement(187..1185)
                     /gene="sirC"
                     /locus_tag="POB92_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDI6578900.1"
                     /translation="MTEKINKKDNYHLIFALIFLALVSVVSMMSGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAIAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1182..2177)
                     /gene="sirB"
                     /locus_tag="POB92_001393"
     CDS             complement(1182..2177)
                     /gene="sirB"
                     /locus_tag="POB92_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDI6578901.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIAIGLGQNIKLIKWMIIMLISMLAGISVAVAGAIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2193..3185)
                     /gene="sirA"
                     /locus_tag="POB92_001394"
     CDS             complement(2193..3185)
                     /gene="sirA"
                     /locus_tag="POB92_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDI6578902.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3416..4396
                     /gene="sbnA"
                     /locus_tag="POB92_001395"
     CDS             3416..4396
                     /gene="sbnA"
                     /locus_tag="POB92_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDI6578903.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4393..5403
                     /gene="sbnB"
                     /locus_tag="POB92_001396"
     CDS             4393..5403
                     /gene="sbnB"
                     /locus_tag="POB92_001396"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDI6578904.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5424..7178
                     /gene="sbnC"
                     /locus_tag="POB92_001397"
     CDS             5424..7178
                     /gene="sbnC"
                     /locus_tag="POB92_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDI6578905.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSDITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7171..8427
                     /gene="sbnD"
                     /locus_tag="POB92_001398"
     CDS             7171..8427
                     /gene="sbnD"
                     /locus_tag="POB92_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDI6578906.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8417..10153
                     /gene="sbnE"
                     /locus_tag="POB92_001399"
     CDS             8417..10153
                     /gene="sbnE"
                     /locus_tag="POB92_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDI6578907.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKNFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10062..11912
                     /gene="sbnF"
                     /locus_tag="POB92_001400"
     CDS             10062..11912
                     /gene="sbnF"
                     /locus_tag="POB92_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDI6578908.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGAGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11887..12663
                     /gene="sbnG"
                     /locus_tag="POB92_001401"
     CDS             11887..12663
                     /gene="sbnG"
                     /locus_tag="POB92_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDI6578909.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            12663..13865
                     /gene="sbnH"
                     /locus_tag="POB92_001402"
     CDS             12663..13865
                     /gene="sbnH"
                     /locus_tag="POB92_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDI6578910.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITLSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13869..14633
                     /gene="sbnI"
                     /locus_tag="POB92_001403"
     CDS             13869..14633
                     /gene="sbnI"
                     /locus_tag="POB92_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDI6578911.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14829..15236
                     /locus_tag="POB92_001404"
     CDS             14829..15236
                     /locus_tag="POB92_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578912.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFFSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15286..15912
                     /locus_tag="POB92_001405"
     CDS             15286..15912
                     /locus_tag="POB92_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6578913.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSTADYSIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVSTADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEFKNPTTKNLEGLNEEIHKDEATTKVDSDNTQPPIESKDHVSKKD"
     gene            16124..16900
                     /locus_tag="POB92_001406"
     CDS             16124..16900
                     /locus_tag="POB92_001406"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDI6578914.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17044..17166
                     /locus_tag="POB92_001407"
     CDS             17044..17166
                     /locus_tag="POB92_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578915.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
BASE COUNT         5781 a         2767 c         3410 g         5246 t
ORIGIN      
        1 aatattgatt gatattatag aatatgagtc ggtgttattt gtatataaag tattaatatg
       61 taattaagaa agtcgacgct tgttaacaat aacaacaact atatgtataa aaaaacctcg
      121 caactgttag ttaactgaat catttagcta acttcgttgt gaggtcattt tgttttaata
      181 atatcgttat aactttttca cggttaataa taagtatatg aagaatgggg caccaaaagc
      241 agcaataaat acacctgctg gcacttcttt aggcaagaat aaggtacgcc caattaagtc
      301 tgcaataaca attgatatgg caccaatcat tgctgacatt agtaactttt tagcataact
      361 tccgcgaacg attgttttcg cgatatgtgg tgcgattaaa ccgacaaacc caatgttacc
      421 tactaaactg attgccatag atacgagtat agtagaagtg attaattgga ttagtttcat
      481 acgttgtaca tgtaagccta agccaatcgc tacagggtca tcaagtatag atattttcat
      541 ttttggtata acaagaaata acaacggcac aacagctaaa ataaccatac ccaagatgat
      601 tgtatcttta aacgtagcac cgtaaagact tccgactagc catgtataag ctttggcagc
      661 agataattgc ttcgttgtaa tgagtaatcc ttggacaagc gcaataaaca acgtttgcat
      721 cgaaataccg atgattatga gtgttgtcgg gcgtatttgt cctttcgttt gaaacactaa
      781 tagtatcatc attgcaattg cgccacctaa tacagcgaat agtggaagta aatgtatcgt
      841 taaatggctg aaaaatgcaa taaagacaac ggcacttaag ctagcaccac ctgtgatacc
      901 gataatatca ggtgaggcaa ttggattttt taatacattt tgcaacatta aaccactcat
      961 tcctagtgcg gcacctgcta aaatcgcaag tgtaatgcga ggtaagcgta atacttctaa
     1021 agtgaattga tccatactgt catttggatt tataaagtac atcagtacgc gttgtaatgg
     1081 tataaagctt gaaccactca tcatacttac cactgaaacg agggctaaaa agattaacgc
     1141 gaagatgaga tggtaattgt cttttttatt aatcttttcg gtcataagcg ttgacgtcct
     1201 ttcttcataa tatagattaa gacaatagcg ccaatgacag cggtaacgac accgataggc
     1261 aactctagtg gcttaattat tatacgagca acaatgtctg aaatgatcat taggattgct
     1321 ccagctaatg cagtaaaagg aattaaatac ttatagtttg gtggtaataa tcgtttgcta
     1381 atattcggta cgataagacc cacaaagacg attgctccag ctacggctac cgaaataccg
     1441 gctaacatac tgatgagcat aataatcatc catttgatta attttatgtt ttgaccgagg
     1501 ccgattgcaa tgtcgtcact tgtcatcaag atgttgatgt gtgcagccat gctaaatgca
     1561 attaaaataa gtatcaatac aagcggaata atccatggga tatcccaaat attacgtaat
     1621 gaaacggagc cacttaacca aaataatagg ccttgtaagt ctgtttcgtt cataataagt
     1681 atgccttgag taaaggctgt aaatagcatc gcaatcgcag cacctgccaa aatgacacgg
     1741 tgaggtgaga atagtgtttg tctaaacata cctagcgcaa caactaatac agtaacaaca
     1801 atggcaccta aaaatgcaat aactacaatc attttaaaag attgaatttg gataaatgta
     1861 atactaaaaa tgacaaaaaa tactgcgcct gcattgacac cgaaaagccc tggtgatgct
     1921 attgggtttc gtgtaagtgc ttgcatcaac aaacctgaga cagcaagggc agcaccagtc
     1981 aataacgcaa tgattgttct cgacgcccgt gcaccagtga caacatcatg taaatcgttt
     2041 tcactatcaa agttgaataa cgcctgtatc accgtacctg gtgacacaag cgtatttcca
     2101 atcattaaac ttaagatagc tactattgca agacataaac cagcaataac gatttggtat
     2161 tttggtttaa gtagcatcgt aaaactcctt aattattttg attgtttttc aatatttaac
     2221 ttttcatata aatcgtcaat caattttaat gatgatttat atccgccagc taagttccaa
     2281 gtgatttcat ctaaatcatc agatacttgg ttgtttttaa ctgcgtctaa atttttccat
     2341 tctttacttg aagtccattc gctttcagtc tttttaacta atgcagcatc tttcgcattt
     2401 ggatctgatt ttactacaaa aatatgatca gcgttcatta atggaatgct ttctttggat
     2461 gtaagttgga taatatcttt accattatca acttgttttt gtaagtcttt attacgtttg
     2521 aatcctaaat catttaagat ttcaccagca tatccaccag cataaattct tgtatgatca
     2581 gcacggaagt taacaactga agctttcaat ggccatgcat ctttatactt tgcttttgca
     2641 tctttttgga atgcagctac tttatcatcg tactttttaa gtaaatcttc agcttctttt
     2701 tctttcccta aagctttccc cattaactta gttgtatctt tgaatttgaa aactgtatca
     2761 gtagaaactg ttggtgcgat tttagataat tgatcgtaaa ctttttcatt tctaactttt
     2821 gacgcgacaa ttaagtccgg ttttaattta gagatttcct ctaagttagg tgcaggttct
     2881 tgacctacaa tcttagtatc ttttaaatca ttttttatgt attcgaattt cggtttttgt
     2941 gtccatgatt ctacagcacc tacaggttta acacctaaag atacagcgac gtcagtggca
     3001 ccttgatata gcgtaacaac acgctttggt ttccctttaa tttcagttgt acccattgca
     3061 tgtttaattg aagttgtttc cttatctttg ttatcagatg attgtttatt tgaattccca
     3121 ctacatcctg ctaaaacaag taggaaagca agcgtaacaa caagcatttt aattacttta
     3181 ttcattgact aattagcctc cttcgtgatg tatgacaatg agaatcatta tcacgattta
     3241 gtatgaatta aattttttcc taagtcaata aaatatttat gatttacatg caacttataa
     3301 ttatttgaca tataaatgca taaaaaatat aatcctaatt acttgatagt gagaatcatt
     3361 atcaattagg taacacacaa tattatagaa ttttaaattt gaggaggaag cgcttttgat
     3421 tgaaaaaagt caagcatgtc acgattcatt gttagattct gtagggcaaa cacctatggt
     3481 tcaacttcat caactatttc cgaaacatga agtgtttgca aagttagagt atatgaatcc
     3541 tggaggcagc atgaaagatc gacctgccaa gtacatcatt gaacatggta ttaaacatgg
     3601 tttaatcact gagaatacac atttaattga aagtacttct ggtaatttag gcattgcgtt
     3661 ggcaatgata gctaaaatca agggattaaa actcacgtgt gttgttgatc ctaaaatatc
     3721 accaacaaat ttgaaaatta ttaaaagtta tggtgccaat gtagaaatgg ttgaagaacc
     3781 tgatgcacat gggggttatt taatgactcg tattgcaaag gtgcaagaac tgttagccac
     3841 tattgacgat gcatattgga ttaatcaata tgcgaatgag ttaaattggc aatcccatta
     3901 tcatggtgca ggcacagaga ttgttgaaac aattaagcaa cctatagatt attttgtcgc
     3961 gccagtcagc acgacaggta gcattatggg tatgagtaga aaaataaaag aagtgcatcc
     4021 aaacgcacaa attgttgctg ttgatgcgaa agggtcagtc atttttggtg acaaacctat
     4081 taatagagaa ttacctggta tcggtgctag tcgtgtaccc gaaatattga atagatcaga
     4141 aattaatcaa gtgatccatg tagatgatta tcaatctgct ttgggctgtc gaaaactgat
     4201 tgattatgaa ggcatatttg ccggaggttc aacaggttcg attattgcag cgattgagca
     4261 gttgataacg tcaattgaag aaggtgcaac aattgtcacg attttaccag atcgaggcga
     4321 tcgttactta gatttagttt attcagatac atggttagaa aaaatgaaat caagacaagg
     4381 agttaaatca gaatgaatag agagatgttg tatttaaata gatcagatat tgaacaagcg
     4441 ggaggtaatc attcacaagt ttatgtggac gcattaacag aagcattaac agcccatgcg
     4501 cacaatgatt ttgtacaacc gcttaagccg tatttaagac aggatcctga aaatggacac
     4561 atcgcagatc gaataattgc aatgccaagt catatcggtg gtgaacacgc aatttcaggt
     4621 attaagtgga taggtagtaa gcacgacaat ccatcgaaac gtaatatgga gcgtgcaagt
     4681 ggtgtcatta ttttgaatga tccagaaacg aattatccaa ttgcagttat ggaagcaagt
     4741 ttaattagta gtatgcgtac tgcagcagtt tcagtgattg cagctaagca tttggctaaa
     4801 aaaggattta aagacttaac aatcattgga tgcgggctaa tcggagacaa gcaattacaa
     4861 agtatgttag agcaattcga tcatattgaa cgcgtgtttg tttacgatca attctctgaa
     4921 gcatgtgcac gctttgttga tagatggcaa caacagcgtc cggaaattaa ttttattgcg
     4981 acagaaaatg ctaaagaagc agtatcaaat ggtgaagtag tcattacatg taccgtaacg
     5041 gatcaaccat acattgaata tgattggtta caaaagggtg catttattag caacatttct
     5101 atcatggatg tgcataaaga agtctttatt aaagctgaca aagtcgtagt agacgactgg
     5161 tcacaatgta atcgagaaaa gaaaactatt aaccaattgg tgttagaagg taaattcagc
     5221 aaagaagcac ttcatgctga actaggacaa cttgtgacag gtgacatacc aggacgtgaa
     5281 gacgatgatg aaatcatatt acttaatccg atgggtatgg ctatcgaaga tatttcaagt
     5341 gcttacttta tttatcaaca ggcacaacaa caaaatattg ggacaacatt gaacctatat
     5401 taagaatgcg aggtgtctga acattgcaga atcatacagc agtcaataca gcacaaacga
     5461 taatattaag agatttagtt gatgcattat tatttgaaga tatagccggt attgtatcga
     5521 atagtgatat tactaaagaa aatggacaaa cgcttttgat atacgaacgt gaaacacaac
     5581 aaataaagat acctgtttat tttagtgctt taaatatgtt tcgttatgaa agctcacaac
     5641 caattacgat agagggaagg gcgtctaagc aacctttaac agcagctgaa ttttggcaaa
     5701 caattgctaa tatgaattgt gatttaagtc atgaatggga agtggctcga gttgaagaag
     5761 gactgactac tgctgccaca cagcttgcta aacaattatc agaattagat ttagcgtcac
     5821 atccttttgt gatgtcagag cagtttgcaa gtttaaaaga tcgtccattt catccattag
     5881 ctaaagaaaa aagaggatta agagaagcgg attatcaagt gtatcaagct gaattaaatc
     5941 aatcatttcc tttaatggtt gcagcagtta aaaagacaca tatgattcat ggcgatactg
     6001 caaatatcga tgaattagaa aatttgacag cacctataaa agaacaagcg acagacatgt
     6061 taaatgatca agggttatca atagatgact atgtactatt tccggtacat ccttggcaat
     6121 atcagcatat tttgccgaac gtctttgcga aagagattag tgaaaagttg gttgtactat
     6181 taccgttaaa atttggagat tatctgtcgt cttcaagtat gcgttcatta attgatattg
     6241 gcgcaccgta taaccatgtc aaagtaccat ttgcaatgca gtcattaggg gcattaaggc
     6301 taacgcctac gcgttacatg aaaaacggag aacaagcaga acaattatta cgtcagctta
     6361 tagaaaaaga tgaagcacta gctaagtatg tcatggtttg tgatgaaaca gcttggtggt
     6421 catatatggg tcaagataat gatattttca aagatcaatt aggtcatcta actgttcagc
     6481 taagaaaata tcccgaagtg ctagccaaaa atgatacgca acagctagtg tcaatggcag
     6541 cactcgcggc aaatgatcgc actttatatc aaatgatttg tggaaaagat aatatttcta
     6601 aaaatgatgt catgacgtta tttgaagata tcgcgcaagt ctttttaaag gtaacactat
     6661 catttatgca atacggcgca ttaccagagt tgcatggtca aaatatattg ttgtcatttg
     6721 aagatggacg tgtacaaaaa tgcgtgttac gtgatcatga tactgtcaga atttataaac
     6781 catggctaac agcacatcag ctttcattgc cgaagtatgt cgtcagagaa gatacaccta
     6841 atacgctaat taatgaagat ttggaaacat tctttgctta ttttcaaaca ttagctgtat
     6901 cggtaaatct atatgccatt attgatgcaa ttcaagattt atttggtgta agtgagcatg
     6961 aacttatgtc gttgttaaaa caaattttaa agaatgaagt ggcaactatt tcctgggtta
     7021 caactgatca gctagctgtc agacacattt tatttgataa acagacgtgg ccattcaaac
     7081 aaattttatt accattgcta tatcaacgtg atagtggtgg aggtagtatg ccttcaggtt
     7141 taactaccgt accaaatcca atggtgacat atgattaatc agtctatatg gcgcagtaac
     7201 tttcgcattt tatggctcag tcagtttata gcgattgctg gactgacagt acttgtgcca
     7261 ttattgccaa tttatatggc atcactacaa aatctatcag tcgtagaaat acagttgtgg
     7321 agtggtatag cgattgctgc tccagctgta acgacgatga tagcttcgcc gatatggggg
     7381 aagctaggtg ataagattag ccggaaatgg atggtgttaa gagcgttact tggtttggcg
     7441 gtatgcttat ttttaatggc attgtgtacg acaccattac agtttgtact tgtgaggtta
     7501 ttgcagggac tatttggtgg tgttgttgat gcatcaagtg cgtttgcgag tgcagaggcg
     7561 ccagctgaag atcgtggaaa ggtattagga agactgcaaa gttcagtcag cgcagggtct
     7621 cttgtggggc cattaattgg cggtgttaca gcttcgatat taggttttag tgcgttactg
     7681 atgagtattg ccgttattac ttttattgtc tgtattttcg gtgcattaaa attgattgaa
     7741 acgacacata tgccaaaatc acaaacacca aatattaata aaggtattcg ccgttcattt
     7801 caatgtctat tatgcacaca acaaacatgt cgatttatta tcgttggcgt tttagcaaac
     7861 tttgctatgt atggcatgct aactgcatta tcaccacttg cttcatcagt gaatcataca
     7921 gcgatagatg accgtagtgt gattggattt ttacagtccg cattttggac ggcttcgata
     7981 ttaagcgcgc ctttatgggg acgctttaat gataaatcat atgttaaatc agtatatata
     8041 tttgccacga ttgcatgtgg ttgtagtgcg atactgcaag gtttagcgac gaatatagag
     8101 tttttaatgg ctgcaagaat acttcaagga ttaacatata gtgcattgat tcaaagtgtc
     8161 atgtttgttg tcgtgaatgc gtgtcatcaa caacttaaag gcacatttgt tggaacgacg
     8221 aacagtatgt tagttgttgg tcaaattatt ggcagtctta gtggcgctgc cattacaagt
     8281 tatactacac cagctactac gtttatcgtt atgggcgtag tatttgcagt aagtagttta
     8341 tttttaattt gttcaaccat cactaatcaa atcaacgatc acacattaat gaaattatgg
     8401 gagttgaaac aaaaaagtgc aaaataaaga attaatacaa catgcagcgt atgcggctat
     8461 cgaacgcatt ttaaatgaat attttagaga agaaaattta tatcaagtac cacctcaaaa
     8521 tcatcaatgg tctatacaat tatcagagct cgaaacttta acgggtgaat ttcgctattg
     8581 gtctgcgatg gggcatcata tgtatcatcc agaggtatgg cttatcgatg gaaaaagtaa
     8641 aaaaataaca acttataaag aagcaattgc gcgtattttg caacatatgg ctcaaagtgc
     8701 agataatcaa acggcagtgc aacaacatat ggcgcaaatt atgtctgaca tcgataatag
     8761 cattcatcgc acggcgcgtt atttgcaaag taacacaata gactacgtag aggatcgtta
     8821 tatcgtttca gaacaatctt tatacttagg tcatccattt catccgactc ctaagagtgc
     8881 aagtgggttt tcagaagcag atttagagaa atatgcaccc gaatgtcata catcattcca
     8941 attgcattat ttagctgtgc atcaagatgt tctgctcacg cgctatgtag aaggtaaaga
     9001 agatcaggtt gagaaagtgt tgtatcaatt agcagacata gatatatcag agatacccaa
     9061 aaattttatt ttattaccaa cacatcctta tcaaatcaat gtgttgcgac agcatccaca
     9121 gtatatgcaa tatagtgaac aaggtttaat aaaagacctt ggcgtttccg gtgattcagt
     9181 gtacccgacg tcttcggtta gaactgtatt ttcaaaagca ttaaacattt atttaaaatt
     9241 accgatacac gttaaaatca ctaattttat acgtacgaat gaccttgaac agattgaacg
     9301 gacaattgat gccgcgcaag ttatcgcatc agtcaaagat gaggttgaaa caccccattt
     9361 taaattgatg tttgaagaag gatatcgtgc attgttaccg aatccattag ggcaaacagt
     9421 tgaacctgaa atggatttat taacaaatag tgccatgatt gttcgtgaag ggataccgaa
     9481 ttaccatgct gataaagata ttcatgtatt ggcgtcatta tttgaaacga tgcctgattc
     9541 accgatgtct aagttatcac aagtgattga gcaaagtggt ttagcaccag aagcatggct
     9601 tgaatgttat ttgaatcgta cattattgcc gatattaaag ctgtttagta acacaggcat
     9661 tagtctagaa gcacatgtac aaaatacatt aattgaatta aaagatggca tacccgacgt
     9721 atgctttgtc agagatcttg aaggcatttg tctatctaga acgattgcta ctgaaaaaca
     9781 acttgtgcca aatgttgtgg cagcatcaag ccctgttgta tatgcacatg atgaagcatg
     9841 gcatcgtctt aaatattacg ttgtagtaaa tcacttagga catttagtat caactattgg
     9901 taaagcgact agaaatgaag ttgtgttatg gcaacttgta gcgcatcgtc ttatgacttg
     9961 gaaaaaagaa tacgcgaata acgcagtgtt tgttgactgt gtagaagatt tatatcaaac
    10021 gccgaccatt gcggctaaag cgaatttgat gagtaaattg aatgattgtg gtgcaaaccc
    10081 tatttataca catataccaa atccaatttg tcataacaag gaggtatcgt attgtgaatc
    10141 aaacaattct taatcgtgta aagactagag tgatgcacca actggtatca tcacttattt
    10201 atgagaatat tgttgtgtat aaagcgtcat atcaagacgg tgccggtcat tttacaatag
    10261 aaggacatga ttcagagtat cgttttactg ctgaaaagac acatagcttt gatcgtatac
    10321 gtatcacatc accaattgag cgtgtcgtag gagatgaggc agatacaaca acagactata
    10381 cacaattatt gagagaggtt gtatttacat ttcctaaaaa tgatgaaaag ctagaacaat
    10441 ttattgttga gttattacag acagaattaa aagatacaca aagtatgcag tatcgagaat
    10501 caaacccacc agcaacacct gagacattta acgactatga attttatgcg atggaagggc
    10561 atcagtatca tccaagttac aaatcacgtt taggatttac gttgagtgat aatttgaaat
    10621 ttggtcctga ttttgtacca aacgttaaac tgcagtggtt agctatcgac aaagataaag
    10681 tagaaacgac ggtatcaaga aatgttgtag ttaacgaaat gttacgtcaa caagttggcg
    10741 ataagactta tgaacatttt gtacagcaaa ttgaagcgtc tggcaaacat gtaaatgatg
    10801 ttgagatgat acctgtacac ccatggcagt ttgaacatgt catccaagtt gatttggctg
    10861 aagaaaggct taatggcaca gtactatggt taggggaaag tgatgagcta tatcatcctc
    10921 aacaatcgat tcgtacgatg tcgccaatag acacgacaaa atattattta aaggtaccaa
    10981 taagtataac gaacacttca acgaaacgag tgttggcgcc tcatacaatt gaaaatgcag
    11041 cgcaaattac ggattggtta aagcagatac agcaacaaga tacgtattta aaagatgaat
    11101 taaagacagc ttttctaggg gaagtcttag gacagtctta tttaaataca caactttcgc
    11161 cttataaaca aactcaagtt tatggtgcgt taggtgttat atggcgtgaa aatatatatc
    11221 acatgttaat cgatgaagag gatgcgatac catttaatgc actttatgca agtgataagg
    11281 atggtgtacc attcattgaa aattggatta aacaatatgg ttctgaagct tggacaaagc
    11341 aatttttagc tgtagcgatt cgtccaatga ttcatatgct ttattatcac ggtattgcct
    11401 ttgaatcgca tgcacaaaat atgatgctca ttcatgaaaa tggttggcct acacgtattg
    11461 ccttaaaaga tttccatgat ggtgttcgtt ttaagcgtga gcatttaagt gaagctgctt
    11521 cacacctgac attaaagcca atgccagaag cacataaaaa agtgaatagt aattcattta
    11581 ttgaaacaga tgacgaacgt ttagtacgcg actttttaca tgatgcattt ttctttatta
    11641 atatcgccga aatcatctta tttattgaaa agcaatatgg tatcgatgag cagcgacaat
    11701 ggcaatgggt taaagacatt atcgaggcgt atcaagaagc atttccagag ttgaataact
    11761 atcaacattt cgatttgttt gaacctacga ttcaagttga aaagttaacg acacgtcgat
    11821 tattaagtga ctccgagtta agaattcatc atgttacaaa tccattaggt gtaggaggta
    11881 tcaatgatgc aacaactatc tctgaaacat agattaaaca atggtgattc agtttatggc
    11941 atttttaatt ctataccgga cccattgatg atcgaggtta tcgcagcaag cgggtatgac
    12001 tttgttgtga ttgatacaga acacgtggcg attaatgatg agacactagc gcatttaatt
    12061 cgtgcagctg aagcagcgca tattatacca attgtacgtg tcactgcagt gatagataga
    12121 gatatcatta aagtgttaga tatgggtgcg agaggtatta ttgtgccaca cgttaaagat
    12181 cgtgagacag ttgagcatat tgtgaaatta agtcgttatt acccgcaagg attaagaagt
    12241 ttgaatggtg gtcgcatggc aagatttgga cgtacaccat tacttgatgc aatggagatg
    12301 gctaatgagc atattatggt gattgccatg atagaagatg ttgaaggggt tatggccatt
    12361 gacgatatag cacaagtcga aggtttagac atgatagtcg aaggtgccgc agatttatcg
    12421 cagtcacttg gcataccatg gcaaacgcgt gatgatcaag taacatcaca tgttcaacat
    12481 atttttgagg ttgtgaatgc acatggtaaa catttttgtg cattaccacg tgaagatgaa
    12541 gatattgcaa aatggcaggc acaaggtgta caaacattta ttttaggtga tgatcgcgga
    12601 aaaatatatc gccatttaag tgcatctcta gcgacgtcta aacagaaagg ggatgatggc
    12661 taatgcgtat agttcaacct gttattgaac aattaaaagc acaatctcat ccagtttgtc
    12721 attatatcta tgatttagtc ggactggaac atcatttgca acatattaca ttgtcattgc
    12781 cgagtaattg tcaaatgtac tatgcaatga aagcaaatag tgaacgaaca atcctagata
    12841 caattagtca gtatgttgaa ggattcgaag ttgcatctca aggtgaaata gcaaaaggtc
    12901 ttgcttttaa accagcaaat catattattt ttggaggccc tggtaagaca gacgaggaac
    12961 taagatatgc agtaagtgaa ggtgttcagc gtattcatgt tgaaagtatg catgaattac
    13021 aacggctaaa tgccatctta gaagatgaag ataagacaca acacatttta ttgcgtgtta
    13081 atttagcagg accatttccc aatgcaacgt tgcatatggc aggacgccca acacaatttg
    13141 gtatttctga agacgaagtt gatgatgtca ttgaagctgc gctagcaatg ccaaatattc
    13201 atctagatgg ctttcatttt cattctattt ctaacaattt agactcgaat ttacacgtcg
    13261 atgtagtgaa actttatttt aaaaaagcaa aatcatggtc tgaaaaacat cgatttccac
    13321 tcaaacatat caatcttggt ggtggtatag gggttaacta tgcagattta actagccaat
    13381 ttgagtggga taattttgta gaaaatttta aaacacttat cgttgagcaa gaaatggaag
    13441 atgtgacatt gaattttgaa tgtgggcgct ttattgtggc acatattggt tactatgtga
    13501 cagaagtgct agatattaag aaagtgcatg gtgcttggta tgccatttta agaggaggta
    13561 cgcaacaatt tagactgccg gtatcttggc agcataacca tccttttgaa atttatcgct
    13621 ataaggacaa tccatattca tttgaaaaag tttcaatttc gagacaggac acaacgttag
    13681 tcggtcaatt atgtacaccg aaagatgtct ttgctagaga agtacagata gacgcaatca
    13741 gtacaggcga cgttattgtt ttcaaatatg caggtgcata cggatggtct atttcacatc
    13801 acgatttctt aagccatcca catcctgaat ttatttattt aacgcaaaca aaggaggatg
    13861 aataactatt gaatcatatt catgaacatt taaaattggt accagtagat aagattgatc
    13921 ttcacgaaac attcgaacct ttaagattgg aaaagacgaa aagtagtatt gaagcagatg
    13981 attttatacg tcatcctatt ttagtgacag cgatgcaaca tggtagatat atggttatag
    14041 atggtgtgca tcggtataca agtttgaaag cgttaggatg taagaaagtt ccagtgcaag
    14101 aaatccatga aacacaatat tcaattagta cgtggcaaca taaagttcca tttggtgtgt
    14161 ggtgggaaac gttacaacaa gaacatcgct tgccatggac tactgagaca agacaagaag
    14221 cgccatttat tacgatgtgt catggtgata cagaacaata tttgtatacg aaagatttag
    14281 gcgaagcaca ttttcaagta tgggaaaagg ttgtcgcaag ttatagtggt tgttgttctg
    14341 tagagagaat tgcacaaggt acatatcctt gtctttctca acaagatgta ctcatgaagt
    14401 atcagccatt gagttataag gaaattgaag cggttgttca taaaggggaa actgtgccag
    14461 caggtgtgac acgctttaat atttcaggac gatgtcttaa tcttcaagta ccactggcat
    14521 tacttaaaca agatgatgat gttgaacaac tgcgcaattg gaagcagttt ttagcagata
    14581 agtttgccaa tatgagatgc tatactgaaa aagtatactt ggtggagcaa tagttttact
    14641 gtgatgttga gggaaatatg atgatttagc gtattgatag cgaaaatata ataaaacaat
    14701 atagtgtgga gaacttttga tattttataa atattgaagt tctccatttt tgtattttgc
    14761 atataaaaat taaataaaat aaggtatatt aaggtaaagt ataaatttta aataaatggg
    14821 gaatgagtat gagctcaatt ataggaaaaa tagcaatttg gataggcatc gtagctcaaa
    14881 tatattttag tgtcgttttt gttaggatga tatctattaa tattgctgga ggatctgatt
    14941 acgaaacaat ttttttatta ggattaatat tggctctttt cactgtttta ccaaccatct
    15001 ttactgcgat ttatatggaa agttactctg taatcggagg tgcacttttt attgtttatg
    15061 ctattattgc actgtgttta tataatttct tttcgtcaat tttatggctg attggtggta
    15121 ttttgctgat ttggaataaa tactcaaaag atgaatcgac agacgaaaat gaaaaagttg
    15181 atattgaaag tacagagaat caatttgaat ctaaagataa aatcactaaa gaataaagag
    15241 aatatttaag gcggagtata aatattaaat aaatggggaa tagacatgga aaaaaatgta
    15301 gaaaaatcat tcataaagat aggtttatat tttcaaatag cttatatagt actcatggct
    15361 ataactttat gtgggtttgt aatttgctat ggactaattt tcggcctttt ctatttatta
    15421 tcaggtagca cagctgatta ttcaatagta acaatagtta tatcggcaat aatttctata
    15481 tttgtaatta tactttcaat cgtacctgtc atcgtattgg catctgactt atttaaagaa
    15541 aggatttcaa aaggtgtcat attaattgta ttggctatta tcgctttagt attatgcaac
    15601 tttgtatctg caatactctg gtttgtttca gccatatcta ttttaggtag aaaaaaatta
    15661 gtatctacag cagatactac cactattcaa aaaagtaaag ggaacgcaaa tcaagcatca
    15721 cataaagaca cgtgtaaaaa ggaacttgat agtcaagaca tgatggaaca tcctgagttt
    15781 aaaaatccca cgactaaaaa cctagaagga ttaaacgaag aaatacataa agatgaagct
    15841 acaactaaag ttgacagtga taacacgcaa ccgccgattg aatcaaaaga ccatgtctcg
    15901 aaaaaagatt gatgacaaac taatcgagag acttaaaaaa ataatattca acataagaac
    15961 ttttaaaacg acatttaaac acattgccaa tcactaatgg tagtgcgttt aactatacct
    16021 taaatatctg aatattttgt taaatggagc tacctttgtt gtactattca aatgaagagg
    16081 agtaaaatgt aattaaagga aagtaatttg aggagtgatc tttatgacaa acaacaaagt
    16141 agcgttagta actggcggag cacaagggat tggttttaaa attgcagaac gtttagtgga
    16201 agatggtttc aaagtagcag ttgttgattt caatgaagaa ggggcaaaag cagctgcact
    16261 taagttatca agtgatggta caaaagctat tgctatcaaa gcagatgtat caaaccgtga
    16321 tgatgtattt aatgcagtaa gacaaactgc agctcaattt ggcgatttcc atgtcatggt
    16381 taacaatgcc ggccttggac caacaacacc aatcgataca attactgaag aacagtttaa
    16441 aacagtatat ggcgtgaacg ttgcaggtgt gctatggggt attcaagccg cacatgaaca
    16501 atttaaaaaa ttcaatcatg gcggtaaaat tatcaatgca acatctcaag caggcgttga
    16561 gggtaaccca ggcttgtctt tatattgcag tacaaaattc gcagtgcgag gtttaacaca
    16621 agtagccgca caagatttag cgtctgaagg tattactgtg aatgcattcg cacctggtat
    16681 cgttcagaca ccaatgatgg aaagtatcgc agtggcaaca gccgaagaag caggtaaacc
    16741 tgaagcatgg ggttgggaac aatttacaag tcagattgct ttgggcagag tttctcaacc
    16801 agaagatgtt tcaaatgtag tgagcttctt agctggtaaa gactctgatt acattactgg
    16861 acaaacaatt attgtagatg gtggtatgag attccgttaa taatcatgca ttagcgataa
    16921 ataaatcctt attgttaagt ttaatcactt agcagtaagg attttttagt gcacttagaa
    16981 gggagtgtat tggtagaaaa ttaataagcg aagttcttaa atgagttatg atgtcactgt
    17041 ctaatgcatc agttgaaagc attactagta ttaacacacc caagatatta taaaacatca
    17101 caaaaacacc actatctaat ttatctcaat aaaaattcac aaagttatct cattttattt
    17161 ttataaataa aaaatatcga taaaaagctt acaatacttt atgt
//
LOCUS       DAMZLE010000050        16770 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMZLE010000050 DAMZLE010000000
VERSION     DAMZLE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.050"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            426..791
                     /gene="crcB"
                     /locus_tag="POB92_001408"
     CDS             426..791
                     /gene="crcB"
                     /locus_tag="POB92_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6578916.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            788..1141
                     /locus_tag="POB92_001409"
     CDS             788..1141
                     /locus_tag="POB92_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDI6578917.1"
                     /translation="MISIILVMIGGGFGAITRSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLNIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(1524..2357)
                     /locus_tag="POB92_001410"
     CDS             complement(1524..2357)
                     /locus_tag="POB92_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6578918.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(2569..3477)
                     /locus_tag="POB92_001411"
     CDS             complement(2569..3477)
                     /locus_tag="POB92_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDI6578919.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVTLGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(3602..4795)
                     /locus_tag="POB92_001412"
     CDS             complement(3602..4795)
                     /locus_tag="POB92_001412"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI6578920.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(4901..5012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5167..6759
                     /gene="pckA"
                     /locus_tag="POB92_001413"
     CDS             5167..6759
                     /gene="pckA"
                     /locus_tag="POB92_001413"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI6578921.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVKIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(7137..7883)
                     /locus_tag="POB92_001414"
     CDS             complement(7137..7883)
                     /locus_tag="POB92_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDI6578922.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHS"
     gene            complement(7888..8361)
                     /gene="ytkD"
                     /locus_tag="POB92_001415"
     CDS             complement(7888..8361)
                     /gene="ytkD"
                     /locus_tag="POB92_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDI6578923.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            8427..8684
                     /gene="yidD"
                     /locus_tag="POB92_001416"
     CDS             8427..8684
                     /gene="yidD"
                     /locus_tag="POB92_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI6578924.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(8681..9682)
                     /gene="menC"
                     /locus_tag="POB92_001417"
     CDS             complement(8681..9682)
                     /gene="menC"
                     /locus_tag="POB92_001417"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI6578925.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATVSGLSNKQLESLKATKPARIKLKWTPQIMHQIRVLR
                     ELEFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(9687..11165)
                     /gene="menE"
                     /locus_tag="POB92_001418"
     CDS             complement(9687..11165)
                     /gene="menE"
                     /locus_tag="POB92_001418"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDI6578926.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMKNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITMNGLLDNTMDIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTIIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATLIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(11324..11779)
                     /locus_tag="POB92_001419"
     CDS             complement(11324..11779)
                     /locus_tag="POB92_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDI6578927.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKSDFWKTLKYKKDKITYYVYL
                     IENLDDEVFHLSVLQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            12082..12732
                     /locus_tag="POB92_001420"
     CDS             12082..12732
                     /locus_tag="POB92_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDI6578928.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNRSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDEINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            12813..13808
                     /locus_tag="POB92_001421"
     CDS             12813..13808
                     /locus_tag="POB92_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDI6578929.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSIAPTNAKGTFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QNDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAAASSNSDNVSYEASATTPATTSSANNDSEENEQSSKDED
                     KQNASKSDKSSVEKSESNEETATVESAPQSKPVTSEAPPNQNNHNEDSMYDASTE"
     gene            13882..14508
                     /locus_tag="POB92_001422"
     CDS             13882..14508
                     /locus_tag="POB92_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578930.1"
                     /translation="MKFKAIVAIALSLSLLTACGANQHKENNSKSNDTNKKTQQTDNT
                     TQSNTDKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDHDETDGESEDI
                     QHHDNDNNKAIQNDIPDQKVDDKSDKNAVNKEEKHDNGTNNSEETKVK"
     gene            14549..14890
                     /locus_tag="POB92_001423"
     CDS             14549..14890
                     /locus_tag="POB92_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDI6578931.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSSLGMKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIMQK"
     gene            14991..15563
                     /locus_tag="POB92_001424"
     CDS             14991..15563
                     /locus_tag="POB92_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578932.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSITKGKYTRAGEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYISEYNPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLKEIEIYDFLESR"
     gene            complement(15761..16318)
                     /locus_tag="POB92_001425"
                     /pseudo
     CDS             complement(15761..16318)
                     /locus_tag="POB92_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864138.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
BASE COUNT         5639 a         3036 c         2537 g         5558 t
ORIGIN      
        1 ttaactcaat tgtactaatt ttgtctataa tttttgcaga tcgttcaatt aaatcactaa
       61 agcctgatgc agattggatt agaattgcga gtaaaagttt gccttggatt attctaattg
      121 ttattttagt acatatcttt tcaattgttc gtacattcgg ttttatttaa aaaagataaa
      181 atgtcaatgt agccttaata caaaacaata caatgtatca tgctataatg agtaaaacaa
      241 tttgatgacg ttgttgcgta taaaaaatat tagattttcg aaatcataac tatacatcta
      301 atcgctatag ttatacaaca agatataaca tataatgagg tttgataatg catcgacaat
      361 ttttgtcgtc gcgttgccaa aacctctttt ttaaattcaa actacttctt ttcgaggtga
      421 accaaatgca atatgtatat atttttatcg gtggtgcttt aggcgcttta ttacgttacc
      481 tcatttcttt tctgaatact gacggaggtt ttccaatcgg aacactgata gccaatttga
      541 ctggtgcctt tgtaatggga ttgctaacag ccttaacaat tgcatttttt tcaaaccatc
      601 cgacgctaaa aaaagctatt acgactggtt ttcttggtgc tttaacgact ttttcaacat
      661 ttcaattaga attaatacat atgtttgatc atcaacaatt tataacttta ctactatatg
      721 ctgtaacaag ttatgtcttt ggtattttgt tatgttacgt cggtataaaa ctaggtggtg
      781 gtttatcatg atatcaatca ttttagtcat gattggcggc ggtttcggtg caattactag
      841 aagtgccatt actgattatt ttaatcataa atttacttca aagttaccta tcgcaacatt
      901 gatagtaaat ctagttggta gttttttaat tggattaaat ataggcttat caatttcaat
      961 ctcatggttc cctgcgttct ttgttaccgg ttttttaggt ggcttaacaa ctttctcaac
     1021 gttagccaag gaacttacac taatgatgac gccaaaattt aatattaacc tttttctcaa
     1081 ttattcactt ttacaattca tcattggatt tatagcttgt tatattggct atcatattta
     1141 aaaataaaat gcttcattca gcaaataggt aaattacgac accttcctga acgaagcatt
     1201 ttttaatttt catgcaaatt ttttagcacc ataaaatgcc taccaaattt caataatctt
     1261 tgttaccgtt taaataatgt gaatgtcaat aaattctcca aactagtcga aaataaaggg
     1321 agtgggacag aaatgatatt ttctcaaaat ttatttcgtc gtcccacccc ggcaaggttg
     1381 actagaattg aaaaaagctt gttacaagcg cattttcgtt cagtcaacta ctgccaatat
     1441 aacttcgtag agcatagaac attgatttat gtcccagcct gtgacaatcc atctaacctt
     1501 ttcatcagtt gagtgatttt aatctatcct tcaaaagttt ttggatcagg tccaattctc
     1561 ttatcttgat ttaaaccatc aattcgcgtc atttgttcat ctgataattc gaaatcaaat
     1621 atttggaagt tttcagagat tctgtttggt gttaccgatt tagggattgt aaccacacca
     1681 tgctgcacat tccatcttaa aacaacttgg gcaggtgact ttcctaattc ttgagcaatg
     1741 tctttaattg tctcatcatt taaaatttgt gcattcatca atggtgacca agattccatc
     1801 acgatatgtt gtgctgccaa atataatttc aatttatgtt gcgttaaata tggatgatat
     1861 tcaacctgat taattacagg tttaattgac acttgtgcca acaaagcttc caaatgttca
     1921 ggttcaaaat tgctgacacc tatattttta actttattat ttttatataa atcttccata
     1981 cctttccatg tatcaaccat tacggcttcg ttcgtacctg gccaatgtac tagatacaaa
     2041 tctaagtatt ttaaacctaa tctagataaa ctagcttcgt aagcagctgc tacattttca
     2101 cgaccgaaat cctcaaaata taattttgaa gtaataaata agtcttctct agcaatacca
     2161 gttgactcca atccggcacg aatgccagca cctacttgtt cttcattccc ataaactttt
     2221 gcggtatcaa tactacgata tccttgttca atggcatact taacactttc catgcaattt
     2281 tcatcatttt ccacacgaaa tgtccctaaa ccaatttgtg gcatcgtgtt tccattataa
     2341 aatgttttaa cctccataaa tatcgcctca cctttttgat gtattatacc ctgttatcat
     2401 aacaaatctg agttgaatac atgagaaaaa acacttagag caatcaacca ctaaaattct
     2461 agtaatatct ctcaaatatt aatcaaattg taaaagtaat tctgtttaat ttatgacaaa
     2521 ctaaaaaagc cgaagtaaca acatatagtc atcacttcag cctaacattt aattgaatga
     2581 ttcaatttta tccatcattt gttgtaagtc ttccacgttg tattgaatac gaccatggaa
     2641 tacaaatttg ttaaagaact cgtctaattg ttcagcaccg acaagcactt tgacagcact
     2701 attttgatta taatttgaaa tcgttacatc gccttcattt ttaggattaa agtataaaat
     2761 tgaagttggc gtatatttgg cacctaattc tttttgtaag tcttcagcca attgtttaat
     2821 cgcctcaatt tgatctgaat aatttacaaa agataatgaa cgtttgtcat cattttgatc
     2881 catcacaata gtttgtggtc tagatttatc taaatccaat gtatcaaata cttgttccat
     2941 tggtggtaaa tctttaaatt gaccgccact aataccatta taaacatgac cttttaacaa
     3001 ttgagaatca ataatataaa gaccagttct tgttaatact aaatgactaa ttcgttcaat
     3061 attattaaag ccatcctttg gtaaaaagat atttgccata atgtgcatat cttctggtcg
     3121 aattcgtttt tctttaacta atctttcacg aataccaatt aatctcatgt ccgttacata
     3181 ttcactatga tttttcgaga acaattttaa tgcgtcaatc tcacgatctt ttgtactaac
     3241 catgtgatta taatcttctt gttgttttgt aattgtcttt ttattttgaa tacgctcttt
     3301 ctctaaagct tcttcatgag actttttaat gttttgttct tgttgttcat acttttcttc
     3361 tgtttgtcgc ttaacttttt tcttactacc taaggtaact aagaaaagga caaaaaagat
     3421 taatgcaatg actactgcaa taatgagtcc aatgactatc ggtgaagata aatccatcac
     3481 aacaacgctc ctttttaata tatgaataac tttaattata atagaaaagc taaagatttt
     3541 cgatacatat tatcatttat ataccgaaaa tcttttattt agctatattc aattcatctt
     3601 attattttac tgcgtctttt aattcttcca ctttgtctaa tttttcccat gggaataaga
     3661 catctgtacg tccaaaatga ccataagcag cagtttgttt gtaaatcggt tgtttcaaat
     3721 caagcatttt aataatacct gcaggtctta ggtcaaagtg ttttctaact gcttcgacaa
     3781 gttgcccttc agaaacttta cctgttccaa atgtatcaat tgcaattgac actggttctg
     3841 caacaccaat cgcatatgcc aattgtactt cacattgatc tgctaaacct gctgcaacaa
     3901 tatttttagc cacataacgt gcagcgtatg cagctgaacg gtctactttt gtaggatcct
     3961 taccactgaa gcatccgcca ccatgacgtg catagccacc gtacgtatca acaatgattt
     4021 tacgtcctgt taatcctgca tcaccttgag gtccaccgat tacaaagcgt cctgtaggat
     4081 tgatgtagaa tttagtttgt tcattaatca agttttctgg aacagttgga taaatgacat
     4141 gtgctttaat gtcttcttga atttgttcaa gtgtcacatc ctcagcatgt tgtgttgata
     4201 cgacaatcgt atcaatacgt actgggttat cattttcatc atattcaaca gtgacctgaa
     4261 ctttaccgtc tggtcgtaaa taatttaacg taccatcttt acgcacatct gataaacgtt
     4321 ttgccaattg atgtgataaa taaattgcta gaggcatata cgtctctgtt tcattcgttg
     4381 cgtaaccaaa cattaaacct tggtcacctg cacctgttgc ttcaatttct tcttcgctat
     4441 ctttatcacg atactctaat gctttatcca cgccttgtgc aatgtcaggt gattgttcat
     4501 caatcgcagt taaaattgcc attgtttcat aatcataacc atattttgct cttgtgtatc
     4561 caatttcttt aattgtttct ctaacaactt tcggaatatc aacatatgtt gttgtagaaa
     4621 tttcgccggc gatcaatgcc atacctgttg taacagttgt ttcacaagct acacgtgcat
     4681 ttgggtcgtc ttttaaaata gcatctaata ttgcatctga cacttggtca gcgattttat
     4741 ctgggtgtcc ttctgtaaca gactctgaag taaataatcg tttgttattt aacatagttt
     4801 gctcctttaa atttatatta cgaaaattct ctctctgtga gctaaataaa aaagaccttc
     4861 taactattaa tatagagaga aggcctaata cgtccattcg ctcttatcgt tcagacctat
     4921 ttgtctgcaa acggtttggc acctttcttt tataaaaaaa gaggttgctg ggtttcattg
     4981 ggtccatgtc cctccaccac tcaggataag agaatccgtt aaaaataata gtacctaatt
     5041 aatgaattaa tgtcaatttt tcacaaataa atttacagta aaatattgta gattaattat
     5101 gttaatgtgt tatactaatt aaatgtaaag gcttacattt aaattatcgc tttggaggga
     5161 tttaggatgt cagtagacac atacactgaa acaactaaaa ttgacaaatt actgaaaaaa
     5221 ccaacgtcac attttcaact ttcgacgaca caactttata ataaaatctt agacaataac
     5281 gaaggggtat taacagaact tggtgctgtt aatgcaagta ctggaaaata tactggtcgt
     5341 tcgcctaaag acaaattttt tgtctctgaa ccttcatata gagataacat tgattgggga
     5401 gaaattaatc aacctatcga tgaagaaact ttcttgaagt tataccataa agtactagac
     5461 tatttagata aaaaagatga actatacgta tttaaaggct acgctggtag cgataaagat
     5521 acaatgttaa aacttacagt catcaatgaa ttagcatggc ataatttatt tgctaaaaat
     5581 atgtttatta gacctgaatc aaaagaagaa gctacaaaga ttaaacctaa cttcactatc
     5641 gtttctgccc cacattttaa agcagatcca gaagttgatg gtactaaatc tgaaaccttt
     5701 gtcattattt catttaaaca caaagtcatt ttaatcggcg gtactgaata cgctggtgaa
     5761 atgaaaaaag gtatcttctc tgtaatgaat tatctcttac cgatgcaaga tattatgagc
     5821 atgcattgct cagcaaacgt tggtgaaaaa ggcgatgttg cattattctt tggtctatct
     5881 ggcactggta aaacaacctt atcggctgat ccacaccgaa aactaatcgg tgatgatgaa
     5941 cacggctgga ataaaaacgg ggtctttaat atcgaaggtg gctgctatgc aaaagcaatt
     6001 aatctttcca aagaaaaaga accacagatt tttgacgcaa tcaaatatgg tgcgatttta
     6061 gagaacactg tagttgcaga agatggttca gtggactttg aagacaatcg ttatacagaa
     6121 aacactcgtg ccgcttatcc aattaatcac attgacaata ttgtagtacc atcaaaagca
     6181 gcacatccaa atacaattat tttcttaact gcggatgcat ttggtgttat tccaccgatt
     6241 tcaaagttaa ataaagacca agcaatgtat catttcttga gtggtttcac ttctaaatta
     6301 gctggtacag agcgtggtgt gacagaacct gaaccatcat tctcaacatg tttcggagca
     6361 ccgttcttcc cgttacaccc tactgtttac gctgatttat taggtgaact tatcgattta
     6421 catgatgttg atgtttatct tgttaatact ggatggactg gcggaaaata tggtgtagga
     6481 cgtagaatca gcttacatta cacacgtcaa atggtaaacc aagcgatttc tggcaaattg
     6541 aaaaatgcag aatatacaaa agatagtacg tttggtttaa gcattcctgt aaaaattgaa
     6601 gatgtaccga aaacaatttt aaatccaatt aatgcttgga gcgacaaaga gaaatataaa
     6661 gcacaagcag aagatttaat tcaacgtttt gaaaagaact tcgaaaaatt tggtgaaaaa
     6721 gttgaacata ttgctgaaaa aggtagcttc aacaaataaa tttgaatact aattaaagag
     6781 ccaaccgttg ttatagtcta acaatggttg gctcctctta ttttatgtgc taaaaattta
     6841 taggcaattt tattacaaca atgcacattt aaggtgacct tcatgccaaa ctcgcatcac
     6901 tcatttaatg gaagcagcac gtcttcacat aaagtaccgg ttccttacgc tatgcatgtt
     6961 gccctacttt gcataaagta cttatcgtta actcaacgca agttgttcct attcacaaaa
     7021 agcacataca gttatctgca tggagcacat tttcattgaa agcgcatacc cctcattcaa
     7081 cgtatatcac acatcttcaa agcttgatag ttcccatgcg cacaccacgt ttcatactag
     7141 ctatgcaact caacttggtt cataaactct ttaatataag tcaatgtttc aaccatcgct
     7201 ggtggtcttg gcacatgtcc ttctgccatt tgataaaatg tttcatgcgt ggcacctttt
     7261 aactctagtt ggtccgctaa ataatacgca tgatgaatac caacttgctg gtctttccct
     7321 ccatgtacaa ttaatattgg cggactgttt tcattaatgt ttggaatcgc ttggcgtgcc
     7381 tcatatgccg ctcgatcttt tttcggatga ccaatcattc ttcgtagcat gcctcttaaa
     7441 tcgacacgtt cttcatacat taaatcaata tctgagacac caccccagat tgtataactt
     7501 gttactggta agtcttgaaa tgtcaacaat ccttgtaaac cacctcgcga aaaaccaacc
     7561 atgtggataa atgcatgtgg atatttatca tgtagcaacc ttaataattg cgtcacatca
     7621 tttaaatcgc cacggtaaaa ttcgtcttta ccttcactcc cattgttacc tcggtagtat
     7681 ggcccaatca ctaaagtttg actatctgaa aattgcatta atctacctgc gcgcacacgt
     7741 cctacttgac ctttgccacc tcgcaaatat actacaatgc gatttacttc atgatgtggt
     7801 gtcatcatta aagcttttac ttgtaagtca tctgacaaat atgtaatttc ttcgaattga
     7861 tgcgtaaaag attcaattgg cattcgttta cgtttgataa aacccaagtg attgcaccct
     7921 ctctacgcat tttaaaatgg tactatcttg cagtaagaaa ctccgttgtg cgagttcaat
     7981 atcattgata cagttaaaca acactggccc tgctgtttct aaataatcgt tcttgcttac
     8041 caatgattca acttcgataa aatatacatc ttttacaaaa tcagtttgat catgtgtttc
     8101 aatggtatat tgtgctatgt aatgaatatt tttaactttg gcgcctgttt cttcatataa
     8161 ttcacgggta actgcttcag cactactttc cccgcgttcc cttttaccac caggaaattc
     8221 aataccccgt aaattatgtt tggtaaaaag caattgattt ttaaacgttg gaatagctag
     8281 cacatgattg ccatctgcta tctcattatc ctttttaaat gtcaaattaa cttgacgatt
     8341 atctttatcc ctaaacttca cgcgcatcac atccctacat tgtatgttaa tataatagtt
     8401 aattactatc gttggaggca ttaattatga aaaagatatt cttggcgatg attcattttt
     8461 atcaacgttt catttcgcca ctcactccac caacttgtcg tttttatcca acatgttcag
     8521 agtacactag agaagcgatt caataccacg gtgctttcaa aggcctttat ttaggtatcc
     8581 gtcgtatttt aaaatgtcat ccgcttcata aaggtggctt tgaccctgtt ccgttaaaaa
     8641 aagacaagtc agcaagcaag cattcacata aacataacca ttaatatggt tgtaattgag
     8701 ttatatccac taaagggggg cgaaattcga gtcgcccctc ttttaatatg cctgaatgcg
     8761 ccaccacatc ttgttcaaaa taataacctg ctggtgtaac atctcctgga taatcacctt
     8821 tacgagcaag catcgctgta aaataacggc ttaaaccata ttcgtacatg ccgccaataa
     8881 ccacttttac accatgactt ttcaaagtat caattgccgt ttgcacttta tcaatgccac
     8941 ctagacgaaa tggttttaat acaacaactt tcacattgta taattctatc aaattaatta
     9001 tgtccaacaa cgatgttgcc ttttcatcaa gggctattgg aggtattgtt ccatccacta
     9061 cttcatcaag catggagata tctttaaatg gctcttcgat ataaagaacc tgttcacgcg
     9121 ctaataactg taactgtgtg aaatcttgac gatccaagga ctcatttgca tctataacca
     9181 attgaaagtg aaactctaat tcccgtaaca ctctaatttg atgcatgatt tgaggtgtcc
     9241 attttaattt aattctggcc ggcttagttg cttttaatga ctctagttgt ttatttgata
     9301 agccgctcac tgtcgctcca tatgctactg aaaatgaagg cagtacatga aacatttgat
     9361 acaatgccat gacaatagtt gcccttgcag caggcgtatt ttccaatgaa tctactaatt
     9421 ttagtgctgc ttcatacgtt tcaaatgatt tatttctatt atcttcgaac cattgctcaa
     9481 ttacatgttt cactgaagca attgtttcat gatcatacca atctgtttga aaagcgttac
     9541 attccccgaa atatgcattt cctttatcat caatcaattc gataaacaaa caatcacgat
     9601 gtgttaaagt gactttcggt gttacaattt gtgacttaaa tggctcacta tatttataaa
     9661 aatgcaaagc tgtcaacttc atcaaatcat cctctataca acttatttct ttgtaattta
     9721 cctgttgatg tataaggtaa agtatcaacc ttttcaaagt gtttcggtac tttatatttc
     9781 gctaaatgtt gtgataaata tgcaatcaat tgtgcctttg aaatgtcact ttcactgaca
     9841 aaatataatt taggcacttg tccccaagta tcatcaggat gccctacaca tactgcgtca
     9901 ctgatacctg gaaattgctt cgctaccgtt tcaatttgat atggataaat attttcaccg
     9961 ccactaataa ttaaatcttt acgtcggtca taaatcatga cataaccttc atgatctatt
    10021 tcagcaatgt cacccgtatt aaaataacca ttttcaaacg tacccgttaa atctgttgga
    10081 tacaaatatc cattcatcac attggcacct ttaatcatta attctccatg accttcttta
    10141 ttaggatttt taattttaac gtctacattg gcacttggca tccctacagt gtcaggacgt
    10201 gcatgcaaca tttccggtgt tgctgttaaa aattgtgaac atgtctcagt cataccaaat
    10261 gaattataaa ttggcaggtt atattgtaat gccgtctcta tcaaagtggc agataattta
    10321 gcaccgccga gtaatatttt ttgcaaatta taaggttcat gtaaaccttg ttgcataagc
    10381 caatttaaag tttgtggcac aagcgaaatg tgcgtgatgc gttcattttt aattatcgtt
    10441 aaaatttgtt cggcattgaa tttatcaaca atgcgcacag taaacccttc aataacagct
    10501 cttaaaagta cactgagacc cgaaatatga taaatcggca agacagatag ccaattagta
    10561 tcacgatcaa atcccaagct ctctttacat ccgattgcac tggcataatg attacgaaac
    10621 gtttgcggca ccgctttttg agggccagtt gtccctgatg taaacataat cgatgcaatg
    10681 tcatctaaat taaatgaagt atttaatatg ttggacggcg actctttcgg caccacagtt
    10741 tcattcgatg tatcatattg gatatccatt gtgttgtcca acaaaccgtt cattgtaata
    10801 tccgttccag cgaattcaat atcatccagc gatacaattt gaaaccctcg caattccagt
    10861 ggcaaggtac aaaaaatcaa ttgtacatcg attgacctca tctgattctt catctcatta
    10921 ggtgtcaacc ttgtattaat catcgcaatt tcaatatttg ccaaccaaca agcatgtatt
    10981 aaaatgatcg attgaatcga attatctatg tatagcccga cacgagattg ttgataagcc
    11041 ttgagtcttt tagccaatag actcgcttca cagtataaat tttgataagt ataagattct
    11101 tgaccgtctg ttatcgcaat atgatgtcca ttttgttgtg cttgtttata taaccaaaag
    11161 tccatacgct attcctccaa attcatctac cttataatta taacgatttt atgacattct
    11221 agcagtggtt atgtttaaaa atataaaaaa gtagacgaat tgatgcattg atatgattgt
    11281 tataatgctc aatacatatc gttatatcat tcgtctacta ttatcagtta tttttattta
    11341 attttagtgt cattctgtca ttttgatgtg gtgatttacc cattgttgcc acatcatctg
    11401 caatgtcaat tggtatacga ttcatgtctt gtaatacact taaatggaat acttcatcat
    11461 ctaaattttc aatgagatat acataatatg ttatcttatc ctttttatat tttaacgttt
    11521 tccaaaagtc cgacttgcaa ttcaatacat tatccggaat atattcaata aataagtaac
    11581 gtttgctgcc tactttgtct acgaaatatt ttacagtgcc tttttctata cctcttatat
    11641 gtgcatagtc tgctgaaaag taaatactac ctattgtttc attatgttgt tgtatttcaa
    11701 atcgttggcc tactatttta ttatttgtgc cacaactact taaaaaaatc agtagatata
    11761 gcattaaaca tattttcatc cccttgaatt ttaaagactt tttcaaaagc aacacctcta
    11821 aaaataaata caatatatta tatcaaatag aaattattat tttgttaaat tatgttgtca
    11881 tgttcgtgtc attttgagtc gtaatacaac tttacaatta ttaaattaac acttctacaa
    11941 tccaaaaata tttttaaagt tttgaaaaaa attaaagtat ttccactact aatttggtaa
    12001 aatatgatta tgtgatatag tgaaagcgtt ttacacttaa taactccctc ttaaatgcat
    12061 ccaggtttta tgtagtaaat catgaaaata acatataaat atagaggaga tttacctttg
    12121 aatacagaga acaacaagaa tcaaaaccga tctgttaaaa attctgaaag acgtggcatg
    12181 ttaaaaggat gcggcggttg ccttatttct ttcattttat taataatctt attatcagcc
    12241 tgttcaatga tgtttagtaa taatgacaat tccactagta atcaatcatc aaaaacgcaa
    12301 ttaactcaaa aagatgaaaa taaaaatgaa gataagcctg aggaaaaatc agaaacagca
    12361 acagatgagg atttacaatc aaccgaagaa gtacccgcaa atgaaaatac tgaaaataat
    12421 caacatgaaa ttgatgaaat aacaacaaaa gatcaatcag atgatgaaat taacacacca
    12481 aacgttgcag aagataaatc acaagacgac ttgaaagatg atttaaaaga aaagcaacaa
    12541 tcaagtaacc atcatcaatc cacgcaacct aagacctcac catcaactga aacaaacacg
    12601 caacaatcat ttgctaattg taagcaactt agacaagtat atccgaatgg tgtcactgcc
    12661 gatcatccag catatcgacc acatttagat agagataaag ataaacgtgc atgtgaacct
    12721 gataaatatt aaacaacaag cgaattgaat tcaaattgta tttagcttta tgcactaatc
    12781 acatagtaaa taatgaggga gattttttag gcatgagcaa tcaattcaaa agcgaagaag
    12841 agcgaagaca atgggaacaa ttccaagctt tccaaaatca acaaaaccaa cagaaccagc
    12901 aatacggaca aaagaaatct aaaaaaggat ggttctgggg ctgtggtggt tgtctagtat
    12961 tatttatttt aattatcatc ggtatttcag cttgtacagc tggtattaca ggtaaccttg
    13021 gcggaaatag ttctaaagaa acgaacaaaa cccataaaat tggagagact gtaaaaaatg
    13081 gtgatcttga agtcacagtt aattcagttg aaactatgaa atcagttggt ccatctatcg
    13141 cgccaacaaa tgctaaaggt acatttgtcg ttgctgatgt gacgattaaa aacaaaggta
    13201 aagaagcgtt aacaattgat agttcaatgt ttaaactgaa atctggtgat aaaacatttg
    13261 aagcagataa tacaggctca atgtctgcta atcaaaatga caacggtagt atagaaaatt
    13321 catttttctt acagcgtata aatcctgata gcactgctca aggtaaaatt gttttcgatg
    13381 tgtcagaaaa catagccaac gcaaaagata aaaaattaga agttatttct agtttattta
    13441 gcgtcaagaa gattacattt gatttatccg atgctaaaaa aacatcaaaa gctaaaaaag
    13501 acaagcaaga tacagaagta gctgctgcga gttcaaatag cgataatgta agttatgaag
    13561 cttcggctac tacacctgct acaacttcta gtgcaaataa tgattctgaa gaaaatgaac
    13621 agtctagtaa agatgaagat aagcagaatg cgtctaaaag tgataaatct agtgtagaaa
    13681 aaagtgaatc taatgaagaa actgctactg tagaatcagc acctcaaagc aaacctgtta
    13741 caagtgaagc accacctaac caaaataatc acaacgaaga tagcatgtac gacgcttcaa
    13801 cagaataaaa tttcagggta gctcggctac ccttctttta cggaaaaata agtatacata
    13861 gcaatataag gagataaaaa aatgaaattc aaagctatcg ttgccattgc attatcattg
    13921 tcactattaa ctgcttgcgg tgctaatcaa cataaagaaa ataatagtaa atcaaatgac
    13981 actaataaaa agacgcaaca aactgacaac actacacagt caaatacaga caagcaaatg
    14041 acaccacaag aagccgaaga tatcgttcga aacgattaca aagcaagagg tgctaacgaa
    14101 aatcaaacat taaattataa aacaaatctt gaacgaagta atgaacatga atattatgtt
    14161 gaacatctag tccgcgatgc agttggcaca cctttaaaac gttgcgctat tgttaatcga
    14221 cacaatggta cgattattaa tatttttgat gatatgtcag aaaaagataa agaagaattt
    14281 gaagcattta aaaagagaag ccctaaatac aacccaggta tgagtgatca tgatgaaaca
    14341 gatggtgagt cagaagacat tcaacatcat gacaatgata ataacaaagc aattcaaaat
    14401 gacataccag atcaaaaagt cgatgataaa agtgataaaa atgctgttaa taaagaagaa
    14461 aaacacgata acggtacaaa taattctgaa gaaactaaag ttaaataagg gtatactttg
    14521 attaatcgta atttttagga gaaccgctat gataacattt gaaaatatac aacaacttga
    14581 aaaatacacc ttaatgacta tgcatggtct ttttaatcaa cttaaactcg gtattatttc
    14641 aatcgacaat gcagagcata cgctctttac accttatatg atggaaacac tctcttccct
    14701 aggcatgaaa gacagcattg tcgatttaat tcataaaggg actgaattag aagactttgc
    14761 agcatttaat ttatcaattg aagatacagt tacagtctgt ttacaaagaa ctgaagaact
    14821 attaaatcaa tacaaaaatg tggaattcaa tgacaaaata ttaatcaatt ggcgtattat
    14881 gcaaaagtaa tagacatata aaagtcgaac gtaactaaat ggatattgat tttattcttt
    14941 ctaaattaca agtatttcat tttataaagt ttgaaaagag gtatattgaa gtggagaaaa
    15001 atgaatatat agctaaatat aatgaatata gtcaattatt agacgctaca tactcgcaag
    15061 ccgtagcata ccttttaaat aaatatggcg ctgtaaccga tgactattat aaggaaaaat
    15121 catacacgcg atttttaaat ggagaaatca aaagtattac aaaaggaaaa tacactagag
    15181 ctggtgaagg attatattgt catcacataa gcgaagacaa attccaaaac ttatctgacc
    15241 taagattcat ttccgaattt aagtactcat acaactatca aaagaaagaa aacttagttt
    15301 actgcgattt aatcgagcat ttaattttac atgcaattat tacaaaagaa tccaatggcc
    15361 aatttggtgt ggctggatta tgtcaaatga tcaaaccaac agtcattgat tggtacatta
    15421 gcgaatataa tccaaaacca gcatggatgc aagccaccaa agcacgtgcc tatttgccta
    15481 gaatattagt agagaaacta ctcattaaaa ttgacgatat gttaaaagaa atagaaatat
    15541 atgatttcct tgagtctaga taaatgatta atggagattt attttttgct gttgagattt
    15601 tgttatagat gtttaaaccc ataattaaat atattttata aaaatagacc acgcatacct
    15661 atctttaaac ggacaatgtt tataaatgag tttgcatggt cttgaattgt attagttttc
    15721 ttttggtttt aataaatcga ctagattttc acaatattta tcaaatatgt attcctaaat
    15781 tatacagcct taatccagca gctactttcg aaacttccaa cttagttgat ataaggttca
    15841 atagtttgtt tcattctttt tcagataaac cagaacttaa attgatatta ttgacttcat
    15901 aaaaattata gactaatgcc tctatttgct tttttggcat aagtaagtcg actgaaaact
    15961 gatttacgtc gctttcataa atcatttcat gtaaattctt tagactatta tcgttgttat
    16021 ctttaatttt gtataaataa cggccgagtt cacgagctat tgcaaatctt gtattattaa
    16081 tcgagtgatt attattgata taaatcgttc ttccacttaa ataacccgaa gtattaccct
    16141 ccattttaat atatttaact tttaaattaa gttgaaataa tagcttgtct atgtcaatag
    16201 caaagtgttc agaagtaata aaaagttgat ccattttgtc cttaactaat ggctgaatta
    16261 attgaactat ttctggttct aaaatatctt cataatgaac tttctcaata actttcaatt
    16321 gattcccctc cctaactaaa aaatcgaacc tacatcattt aataaattta aaactatagg
    16381 tgtaaacatt attaaaataa aacccctcca ctactaaatg taacggaggg acatgatttc
    16441 aaaataaaat gtcatttttt ataaatttat tataatattt ttcatcatac tgtacaaccg
    16501 aatctaatcc aacgatcccg aaaagaggaa caacggtggt tctgcttcat aatcactttt
    16561 aactaagtgt tcatttttaa ttatttgatc gacggttttc caatttttta cttcttgctc
    16621 ttttaaaaaa atggctatga aaaattctat cctcatagat ttcttcatag ccaatgctaa
    16681 tttttttcat gtcatttctt caaaattcct aaaaatatat aaactaaaag cataagtaac
    16741 acagcaaaaa ttgatgaata tataattatt
//
LOCUS       DAMZLE010000051        16554 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMZLE010000051 DAMZLE010000000
VERSION     DAMZLE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.051"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(768..1697)
                     /locus_tag="POB92_001426"
     CDS             complement(768..1697)
                     /locus_tag="POB92_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6578933.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1892..2116)
                     /locus_tag="POB92_001427"
     CDS             complement(1892..2116)
                     /locus_tag="POB92_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDI6578934.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(2133..2336)
                     /locus_tag="POB92_001428"
     CDS             complement(2133..2336)
                     /locus_tag="POB92_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578935.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
     gene            2495..3055
                     /locus_tag="POB92_001429"
     CDS             2495..3055
                     /locus_tag="POB92_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6578936.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            3074..5476
                     /gene="mnhA2"
                     /locus_tag="POB92_001430"
     CDS             3074..5476
                     /gene="mnhA2"
                     /locus_tag="POB92_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDI6578937.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            5463..5888
                     /gene="mnhB2"
                     /locus_tag="POB92_001431"
     CDS             5463..5888
                     /gene="mnhB2"
                     /locus_tag="POB92_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDI6578938.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            5885..6229
                     /gene="mnhC2"
                     /locus_tag="POB92_001432"
     CDS             5885..6229
                     /gene="mnhC2"
                     /locus_tag="POB92_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDI6578939.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            6219..7715
                     /gene="mnhD2"
                     /locus_tag="POB92_001433"
     CDS             6219..7715
                     /gene="mnhD2"
                     /locus_tag="POB92_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDI6578940.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            7716..8198
                     /gene="mnhE2"
                     /locus_tag="POB92_001434"
     CDS             7716..8198
                     /gene="mnhE2"
                     /locus_tag="POB92_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDI6578941.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            8195..8497
                     /gene="mnhF2"
                     /locus_tag="POB92_001435"
     CDS             8195..8497
                     /gene="mnhF2"
                     /locus_tag="POB92_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDI6578942.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            8472..8909
                     /gene="mnhG2"
                     /locus_tag="POB92_001436"
     CDS             8472..8909
                     /gene="mnhG2"
                     /locus_tag="POB92_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDI6578943.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            9249..11291
                     /locus_tag="POB92_001437"
     CDS             9249..11291
                     /locus_tag="POB92_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6578944.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(12319..13248)
                     /locus_tag="POB92_001438"
     CDS             complement(12319..13248)
                     /locus_tag="POB92_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDI6578945.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(13245..14081)
                     /locus_tag="POB92_001439"
     CDS             complement(13245..14081)
                     /locus_tag="POB92_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI6578946.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(14075..14818)
                     /locus_tag="POB92_001440"
     CDS             complement(14075..14818)
                     /locus_tag="POB92_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI6578947.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            14940..15584
                     /locus_tag="POB92_001441"
     CDS             14940..15584
                     /locus_tag="POB92_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDI6578948.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(15664..16416)
                     /locus_tag="POB92_001442"
     CDS             complement(15664..16416)
                     /locus_tag="POB92_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDI6578949.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYLMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
BASE COUNT         5544 a         2355 c         2794 g         5861 t
ORIGIN      
        1 aaaaaagtgt ttataaaaca aattattcat tagacacagt gattgtattt ctgggttagc
       61 atttggttta gtcaaaaata tcagcatttt ttataattta ccttaattta atcgccaata
      121 caaggtaatt tatttaatga aacgatttag cgcaattaaa tgtttcgata ttcaatgtgt
      181 gtctaaagag attctttgtt atagcttaac tcttccaagt taacttcttc tactatttat
      241 acccattttt cagaatttta tcactaaaaa tgaaaaattt ttataatttt tttaatttag
      301 taattctcag tgcagtttta atgataagaa aaacacatta atttagtgat taaaaccaat
      361 ctatagcacg ttaaagtgta tatacgttat tccgattgat aacgggtaac aaataataga
      421 aagtacgata attgatcatg cattaaagca cttttatttt tcaaactcga ctcaatgaat
      481 tataaaaagt tgtgcatttt gacttatttt taattaattg aataaccaac tatgaaatat
      541 tagcacgaat aacataattt ttgctattaa tttggtgtaa attattaaaa aataacatta
      601 atttaggtct taagaatgag ttgactatcg aatttgctaa attaaaattt gttgtgtcaa
      661 agtatactac ccaaatcaac gcttttctaa agatttttag accacaaaaa aatagcttat
      721 cacttttaaa aataattaca agttaaaatg ataagctaaa agttaattta aaataatctt
      781 aaaagtataa tgcctgtcaa aatacataat agaccaatag tttttctgga tgtcattgct
      841 attttaggtg aaccaaataa tccaaagtga tctatcaata tgcccattag aatctggcca
      901 aacatcccaa taagtgttgt taatgctgca cccatatgag gcattaagat aatgttagct
      961 gttacaaaag ccataccaag tataccgcca gtaaaataga taggctttaa tttaccgaat
     1021 tttaaatgac ttgtttttag ttttaaagaa cgattaaaaa tagcggttaa aatcaatagc
     1081 gttattgacc caattgtaaa tgatactaat gatgcaaagg ctggtgaatg agtatgactt
     1141 gctaaagcac tattaattgt cgtttgaata ggtggaaaga aaccaaaaat aaatcctaag
     1201 agaagccaaa acagtaaata cttttgatca gttagtaata aattattctt gttaaattga
     1261 ttcattatga cgatgccgac aatgagtaac aatactccaa ttgctttaat taaattaaaa
     1321 tcatgaattg tagcgccaaa taagccaaat gtatcaataa tgacacccat aataatttga
     1381 cccgcaactg ttgcaattac agttaatgtt gcacctaatt ttggcaataa caataaattg
     1441 ccagttaaaa agctaacccc aagcaaacca ccgactaccc atgtgtagtt aaatgattga
     1501 ttattgtaaa agtgaatagt aaatacttct ggattgataa tgatatttaa aataattaaa
     1561 caaattgttc caactgaaaa tgaaatgaat gaagtataga aaggcgattt agtatacagt
     1621 gatagtcttg aattgacaga tgtttggata ggaataagca ttccaactag gacaccaatg
     1681 atatagaaaa gaagcatatg aataaagtct cactttccaa agttaataat aaaatcatat
     1741 gaatacatta tcattataga gaaaatatac aactcattta caataatgtt gaaaaaaaca
     1801 ggacattatc acaacaaaat caccctagac aatcatgatg aaagtctagg gtgttagtat
     1861 ttatactaaa ttaattgcgc tttctttata tctatttctt atcttcaaat aaaacagttg
     1921 ttgttttatc aataatttga gcagaatgta ctttggtgat agtaccacta tttgacttta
     1981 taatatttaa atctactaat gcattcatac tatcacgtgc aacttgctcg gtcactacgt
     2041 tgtttaattg tggtaattgc agttttacag gcttgtctag tgaagattta aaacttaatt
     2101 caagtgtttt agtcatgaat atgtcctcct gattaaattg ataaagattt gatgagttcg
     2161 atattgttat atgtttctcc agtaagtcgc tcaataagtt tgctgaatgt tttaatttgg
     2221 tcgtttgatg catcagggtt aatgttagcg aatcgacgct taaattctgt ttgtttgccg
     2281 ttagcgtcta ctttagtaaa tgataataca atagtgatgt ggtttatttt actcatattt
     2341 taaaacctcc tttcacacta tatatcgaaa taaaataata aaatggctaa ttttattttc
     2401 tatgtttaaa atctataaaa aaggcaatag atatatgtaa ctaaaatata aaatagctat
     2461 gattaagaca cttaaagcaa ggggaggttg agtaatgaat aaagtagaag cgattaaatt
     2521 taatgatgat attgttaaaa tgtatgaagc gctcaagata aaatctgaac gtgactattt
     2581 attctttaag ttagctatac atagtggatt gaaagtatca gaattattaa caattacagt
     2641 ctctcaagtt aagagactaa ttgaaaagtg tacgttatca gaaatgtgta aagcacattt
     2701 tcattcgttg attaaaatta ggttaccaga aacattatcg aaagaactac ttcaatatat
     2761 agaggacagg agtctttcga atgaagacgt tctttttcaa tcactacgaa caaatcaagt
     2821 attatctaga cagcaagcat atcgaataat tcaccaagca tcaattgaag ctggtataga
     2881 taatgtagga ctaacgacat tgcgtaagac atttgcatat catgcttatc aaaaaggtat
     2941 acctatacca gtcattcaaa agtatttagg gcatcaatct gctattgaaa cactaaattt
     3001 tatcggttta gaaaatgagt gtgaacatag tatttatatt tcattacaat tatagaaaca
     3061 aaggaggcta ataatgagtt tggtttattt attaattgct atacttgtga ttatggcgat
     3121 gatacttcta atgtctaaac gtagagcatt ggctaaatat gccgggtaca tagcgttggt
     3181 tgcacctgta atttcatcta tctatttttt gattcaaata ccatcagtag ctaaactgca
     3241 atatctttct acctctattc catggattaa gacattagat attaatttag atttacgttt
     3301 agatggttta agtttaatgt tttctcttat tatttcactt attggaattg cagtattctt
     3361 ctatgcaact caatatttat cctctcgaaa agacaattta ccaaggtttt atttttattt
     3421 aacgttattt atgttcagta tgattggtat tgtattatca gacaatacga tattgatgta
     3481 cattttttgg gaattaacga gtgtatcatc atttttattg atttcatatt ggtataacaa
     3541 cggagatagt caatttggtg cgatgcaatc atttatgatt acagtatttg gtggtttggc
     3601 attattagtt ggttttatca tgttgtatat tatgacagga acgaataaca tcacagagat
     3661 attaggacaa gcagatcata ttaagaatca tggattgttt atccctatga tttttatgtt
     3721 tttattaggt gcatttacaa aatcagcaca atttccattt catatttggc tacctagagc
     3781 aatggctgca cctacacctg taagtgctta tttacattca gccacgatgg taaaagctgg
     3841 tatcttttta ttacttcgat ttacaccatt attaggtctt agcaatatgt acgtatatat
     3901 cgttacgttt gttggtttaa taacaatgtt atttggttca attacagctt taaaacaatg
     3961 ggatttaaaa ggtatcctag cgtactctac aatcagtcaa cttgggatga ttatggctat
     4021 ggtgggtata ggtggcggat atgctcaaca ccaacaagac gcaatagcat ctatttatgt
     4081 atttgtatta tttggtgcgc tatttcatct aatgaatcat gccatcttta aatgtgcgct
     4141 tttcatggga gtaggtattt tagatcatga agcaggttca agggatatac gaattttaag
     4201 tggaatgcgt caactatttc ctaaaatgaa tctagtcatg acgatagcgg ctctatctat
     4261 ggctggagta ccatttttaa atggattttt aagtaaagaa atgtttttag atgcattaac
     4321 acaaactgga caattatccc aatttagttt gatttcaatg atagctatcg tgtttgttgg
     4381 tgttattgcg agtgttttta cattcacata tgcactatac atggtaaaag aagtattttg
     4441 gacaaaatat gattctaagg tttttactaa aaaaaatatc cacgaaccat ggttgtttag
     4501 tttaccatct cttatattaa tggtgctagt acctgtaatc ttttttgtac caaatatatt
     4561 tgggaagggg attatcgttc tagcattaag agctgtatca ggtggtaatc atcaaattga
     4621 tcaattggca ccacatgttt cgcaatggca tggatttaac ataccgcttc ttttaaccat
     4681 catcattatt ttattgggta gtgtactagc aatcaaagta gattggaaaa aagtgttcac
     4741 aggtaaaatt agacagattt cagtttcaaa aagctatgag atggtatatc gacattttga
     4801 aaagtttgct acgaagcgat ttaaacgtgt tatgcaagat cgtttaaacc aatacattat
     4861 tatgacctta ggcatattta tgattatcat tggatatggt tatattcgaa ttggacttcc
     4921 taaagtacat cagttacatg tttctgaatt tggggcatta gaaattatat tagcaatcgt
     4981 aactgtcaca attggtattt ctttaatttt tatacgtcaa cgactgacaa tggtcatttt
     5041 aaatggagtc atcggatttg ttgtgacctt attctttata gcaatgaaag cccctgatct
     5101 agcattgact cagctagtag ttgaaacaat aacgacgata ctatttattg tcagtttttc
     5161 aagattacca aacgtgccaa gatctaacgc taacaaaaaa agagaaataa ttaaaatttc
     5221 tgtatcactc ttgatggcac ttattgttgt atcattaatt tttattacac aacaaacaga
     5281 tggtttatca tcaatatcag acttttattt aaaagctgac aaactaacag gtggtaaaaa
     5341 tattgtaaat gcgatacttg gtgactttag agcattagat acattatttg aaggattagt
     5401 gttaattatt actgggctag gtatttacac attattaaat tatcaagatc ggaggggaca
     5461 agatgaaaga gaatgatgtc gtgttaagaa cggtcacgaa acttgttgta tttattttat
     5521 tgactttcgg attctatgtc ttcttcgcag gtcataataa tcctggtggt gggtttattg
     5581 gtggtttaat atttagttca gcgtttattt taatgtttct ggcttttaat gttgaagagg
     5641 ttttagaaag tttaccgatt gattttagaa ttttaatgat tattggagca ttggtatcat
     5701 ctattactgc gataatacct atgttttttg gaaaaccatt tttgtctcaa tatgaaacaa
     5761 cttggatact tccaatttta ggacaaattc atgtaagtac aataacactt tttgaattag
     5821 gtattttatt ctcagttgtt ggtgttattg tcacagtgat gttgtcgctt agcggaggtc
     5881 gatcatgaat ttaatattat tactagttat aggattttta gtgtttatag gaacatatat
     5941 gattttatca atcaatttaa ttcgtattgt aatcggaatt tcaatatata ctcatgctgg
     6001 taatctcatt attatgagta tgggaacgta tggttctagt agatcagaac cactaataac
     6061 tggtggaaac caattgtttg ttgatccctt gttacaagct attgtactaa ctgcaatagt
     6121 tatagggttt gggatgactg cgtttttact tgtacttgtt tatagaactt ataaagtaac
     6181 aaaagaagat gaaattgaag gcctaagggg ggaagatgat gctaagtaac ttattgattt
     6241 taccaatgtt attaccattc ctttgtgcct taatccttgt atttttaaaa aataatgatc
     6301 gtatttctaa atatttatac ttaggtacaa tgactatcac cacaattatt tcattaatgc
     6361 tattaattta tgttcagcgt caccgtccaa ttacgctaga ctttggagga tggtcagcgc
     6421 cctttggtat acagttttta ggagattctt taagtttaat tatggttaca accgcttcgt
     6481 ttgtgattac tttaattatg gcatacggat ttgggcgtgg cgaacataaa gcaaatcgtt
     6541 atcacttgcc atcgttcata ttatttttaa gtgttggcgt gataggctct tttctaacat
     6601 cagatttatt taatttatac gtcatgtttg aaattatgtt actagcgtca tttgtactca
     6661 ttacacttgg acaatctgta gaacaattac gtgctgcaat tatttatgtt gtcttgaata
     6721 ttattggttc atggctattc ttattaggta taggtttact ttataaaaca gtaggtacat
     6781 taaacttttc acatattgca atgcgtttga atgacatggg agataatcgc actgttacaa
     6841 tgatttcatt aatcttctta gtcgcattta gtgcgaaagc agcgctggtc ctttttatgt
     6901 ggctacccaa agcctacgct gtgttaaata ctgagcttgc agcattattt gcagcgttaa
     6961 tgaccaaagt aggggcctat gcattaattc gattcttcac tttactattt gatcaacata
     7021 atgatctcat acatccattg ctagcaacta tggctgctat aactatggtc atcggcgcta
     7081 taggtgtcat tgcttataaa gatattaaaa agattgcagc ttaccaagtc ataatctcaa
     7141 taggatttat cattttaggt ttaggaacaa acacgtttgc aggtattaat ggtgcaatat
     7201 tttatttggt aaatgacatt gttgtaaaaa cattgctatt ttttattatt ggtagtttag
     7261 tttacattac aggctatcga caatatcaat atttgaatgg cttagctaaa aaagaacctt
     7321 tatttggagt tgcgtttatt ataatgattt ttgctattgg cggcgtgcct ccatttagtg
     7381 gctttccggg gaaagtactt attttccaag gtgcattgca aaatggcaat tatattggac
     7441 tagcgttaat gattattact agtctaattg caatgtacag tttatttagg atactttttt
     7501 atatgtattt tggagataaa gatggggagg aagttaattt taagaaaatc ccgctatatc
     7561 gaaaaagaat tttaagtatt ttagtagttg tggttatcgc aatcggaatt gctgcacctg
     7621 ttgtgttaaa tgttacaagt gatgcaactg agttgaacac gagtgatcaa ttatatcaaa
     7681 aacttgtaaa tccgcatttg aaaggagagg actaaatgaa tcaaatagtt ttaaatatta
     7741 tcattgcatt cttatgggta ttatttcaag atgaagatca ttttaaattc tcgactttct
     7801 tttctggata tctaattggt ttaattgtca tttatatatt acacaggttt ttcagcgatg
     7861 atttttatgt tagaaaaata tgggtagcta ttaaattttt aggtgtttat ttatatcaat
     7921 taataacatc tagcattagc acgattaatt atattctttt taaaacaaaa gatatgaacc
     7981 ctggattact ttcatatgaa acaagactaa caagtgattg gtcaataaca tttttaacaa
     8041 ttttaattat tataactcca gggtctacag taatacgaat ttctcaagac tctaaaaagt
     8101 tttttattca tagtatcgac gtgtcagaaa aagaaaaaga tagtttgtta agaagtatta
     8161 agcattatga agacttaata ttggaggtgt cgcgatgata caaacaataa cacatattat
     8221 gattattagt tcactcatta tttttggaat tgcattaatc atctgtttat ttagattaat
     8281 caagggacct acaacagcag atcgtgtcgt tacatttgat acaacaagtg ctgtcgtaat
     8341 gtcaattgtg ggtgtgttaa gtgtacttat gggcaccgtt tctttcttag attcaatcat
     8401 gctcattgcc attatatctt ttgtaagttc tgtttcaata tcacgcttta ttggtggggg
     8461 gcatgtgttt aatggaaata acaaaagaaa tctttagtct tattgctgct gtgatgttgt
     8521 tgttaggtag ttttattgct cttattagtg caataggtat cgtgaaattc caagatgttt
     8581 tcttaagaag tcacgctgcg acaaaaagtt caactttatc cgtgttatta actttaatcg
     8641 gtgtattaat ttattttatt gtgaatacag gatttttcag tgtgcgttta ttactgtcac
     8701 ttgtttttat taatttaact tcaccagtcg gcatgcactt agtcgctcgc gctgcttatc
     8761 gcaacggcgc ttatatgtat cgaaaaaatg atgctcacac acatgcatca atattattaa
     8821 gttcaaatga acaaaactct acagaagcat tacaattacg tgctgaaaaa cgagaagagc
     8881 atcgtaagaa atggtatcaa aacgattgaa gtgttggcta aattgttaag cataacaatg
     8941 cttttgaaaa tctgttttca aaattaatca ttaaaagagt actttgaaat tggttagtcg
     9001 attttaaggt gctcattttt tgtattataa gttaatgttg ttatgtgaaa gcaagttgtt
     9061 tatttataca aacaattagt gataaaataa aattaaataa ttgcaaattt aagtactgca
     9121 atcgtagaca tatatctata ttaaagtgct tttgtggtcg ctaactcaat ttatcctcta
     9181 aagtataatt acaacgagat gtgatactta attttcaatt aaacaatact ttttagagag
     9241 gtgaaagttt ggaaatattt gaaacaattc ttatatttat agctgttgtg atactaagtt
     9301 cgtttgtcca tactttcata cctaaagtac ccctagcatt tatacaaatt ttcttgggca
     9361 tgttactatt tattacccca atccctgttc aatttaattt tgattctgaa ttgtttatgg
     9421 taacaatgat tgcgcctttg ttatttgtag aaggtgttaa tgtttctaga gtccatttaa
     9481 ggaaatatat taagccagtg atgatgatgg cattaggatt agtcattact actgtgatag
     9541 gtgtaggttt atttattcat tggatttggc cagatttacc tattggagca gcatttgcaa
     9601 ttgctgccat tctttgtcct actgatgcag tagcagtgca agcaatcact aaaggaaagg
     9661 tcttgccaaa aggagcaatg acaattcttg aaggtgagtc attattgaat gatgctgctg
     9721 gtattatttc atttaaaata gctgttggag tattagttac aggtgctttt tcacttgttg
     9781 atgctgttca gttgttttta attgcatcaa ttggtggcgc agtggttggt ttacttatag
     9841 gtatggcatt agtaaggttc cgattaacat tgatgcgtcg aggatatgaa aacattaata
     9901 tgtttacaat tattcaattg ttaacaccat ttgttacgta tttaattgct gaattgtttc
     9961 acgcatcagg aatcattgca gcagtagttg caggacttgt acatggtttc gaacgtgaca
    10021 gaattatgca agtacgtaca caactgcaaa tgagttacaa tcatacatgg aatatactag
    10081 gttatgtttt aaatggcttt gttttttcaa tattaggatt tttagtacct gaagttatta
    10141 ttaaaattat caaaacagaa ccgcacaatt taatcttttt aataggcatc actattgttg
    10201 ttgctttagc tgtctatcta tttagatttg tttgggttta tgtcttatat ccttattttt
    10261 atttagccat cagtccattc caaaaaatga tgactaaaaa tgatgatgat aatccaacga
    10321 ctgagaaacc accaaagcga agtttatacg ctttaattat gacgttatgt ggtgtgcatg
    10381 gaacaatttc tttagcaatc gcattaacgt taccgtattt tttagcaggg catcatgctt
    10441 ttacgtatag aaacgactta ttatttattg catctggtat ggttattatt agtttggtag
    10501 ttgcgcaagt attattgcca ttattaacga aacctgcacc taaaacagta attggcaata
    10561 tgtcgtttaa agttgctaga atttatatat tagaacaagt tattgattat ctaaatcaaa
    10621 aatctacttt cgaaacaagt tttaaatatg gtaacgtgat taaagaatat catgataaat
    10681 tagcattttt aaaaactgta gagaaagatg atgaaaactc taaagaatta gaacgtctac
    10741 aaaaaattgc ttttaatgta gaaacaaaaa cattagagtc tttagtagat gaaggacaaa
    10801 taacgaatag tgtacttgaa aactatatgc gttatgctga aagaacacag gtatatagac
    10861 aagcatcatt aataagaaga atgattgtat tattacgagg tgctttatta aaacgaagag
    10921 tacaaacgag agtgaactcc gcatcttcac ttagtgttac ggataactta atggaattaa
    10981 ataaaattaa taaattagtc cattataatg tggttagtcg tttgtctaag gaaacaacaa
    11041 aagataatac acttgaagtt ggaatggttt gtgacggtta tttaatgcga attgaaaact
    11101 taacaccatc aaatttcttc aactcagcaa gtgaagatac gattactaaa attaaattaa
    11161 atgcattgag agaacaacgt cgcattttac gtgagttgat tgatacagat gaagtatcag
    11221 aaggtacagc gttaaaacta agagaagcca tcaattacga tgaaatggtt attgtagata
    11281 gtatgacgta gttcctaatt atgctaaaag ggattgatga aaaactgaag ggcttttcat
    11341 caatcccttt tattttatgg gaattgaata gatagtttta aactatacga attattaata
    11401 tttgagattt aattgaaata agttttaaaa attggaggag atagattaag cgaagtcatt
    11461 taaaggtgaa gttaagtgta ttcacaaaaa atagccacac tcatatgaca tcggatgagt
    11521 gtggcttaag gatctatggg ggggaggaaa ccatagatgt ttactttgat aggccagatt
    11581 aaatatcaaa gtatgcgatt atttatagct tgatgcaaaa gtggtatgcc tattaaaagt
    11641 tactgcacat agcttttaat attccgttca aaggaaaggg gcatacaatt gaacaatctg
    11701 taatagtact tttaaccagc tatgctaaaa gtctagtagg gagaacagtt gtccaatcac
    11761 ataagaacct ctaacttcgt tagtacgatt aagaaaagct ttttagttag tatgtaatac
    11821 aatttattga cgcgcgtgaa tctcttttat aagagtgtgt agggaatggc gttgtataaa
    11881 ttgtattaga agaacttcta acgcatctct gtggttaaaa gagatgaagg gaacgacagt
    11941 ttaattaaaa ctgcataaga acttctagct tttctctctc gttcaaagag aagcagctgt
    12001 tcgcagttta atcaaaacca cataaagctt ttaactttac tctttgattt aaagagtgat
    12061 aaatgtttac agtttaatta aaactgcata agaacttcta gcttttctct ttcgttcaaa
    12121 gagaagttct aataccacca tatcgtgcga tcgggaacgg tatatatatt aataggaggg
    12181 taatatatat ttaacgcacg atatgggact attagccttc gactttgtta tgttgatgtg
    12241 tggcctaaaa tattggagat accaatattt taggttgcat caacatcaat tcatcttact
    12301 tcattaaaac acagcgtgtt atttcatgct tccgtgtaca gtttcaatat ttgatttcat
    12361 cattttgtag taagagtcac ctttagtgcc ttctttaccg attgaatctg tgtacacttc
    12421 accaaagata tctttcttcg tttcttcaga taaactttcc attgctttct tatcaacact
    12481 tgtttctact aataagtgtt ttaatttgtg ctttttaaca aactcaatag cttgtctcat
    12541 ttgttcaggt gtaccttgtt tttcagtgtt aatttcccaa atataacctg gtgtaatacc
    12601 gtattgtttt gagaagtact tgaaggcacc ttcacttgta atcatggcac gttgttcttt
    12661 tggaatgtca ttaaatttgt ctttactgtc attatttaat ttttccaatt gagcaatgta
    12721 tttgttacct tgcttttcat aatctgcttt atgttttttg tcgttatcga taaatgtttg
    12781 ttgaattgtt tttacgtatt taataccgtt atctaaactt aaccatgcgt gtggatcttg
    12841 tttatctttg ttgccttctt caccgtttaa atagataggt ttaacatctt ttgatactgc
    12901 gataactttt ttatctttta atgatttacc agcctgttct aaggcttttt caaaccaacc
    12961 gttaccagtc tctaaattta atccgttgta taaaataacg tcagcgtcag ttaacttttt
    13021 aatatcttta ggtttaactt catattcatg aggatcttga ccaacaggta caatactatg
    13081 aatatcgacg ttgtctccac caacattttt agccatatca tataaaattg aattcgtcgt
    13141 tactactttt aatttgccat ttgacttatc actgctttgt ttaccaccag taccacatgc
    13201 agcaactaga agtaataagg ctaataataa aggtactaat tttttcatgt taaacttcct
    13261 cgtttctttc tattcgtaaa ttttgtgaaa aataatgtga tgatataaat tacaaacgta
    13321 caaagtacga ttgtcgcacc actaggaatg ttgtaaatat agctgtaata aagtccgaca
    13381 attgaactta tgacacttat taaacttgct ataatcatca ttgagtatag ttttttacta
    13441 attaaaaatg ctgtagatgc aggtgtaatt aataatgcaa ctacaagaat aatacctacc
    13501 gtttgaatac ttgctactgt tactaatgag agtaacaaca tcacaaagta atgtaataac
    13561 gtcgtattta gaccactcat tctactaaac gttggatcga atgtagaaat cattaatgga
    13621 cgatagaaaa taatgattag aataaggacg attgaaccaa tcacaatagt tgttaaaaat
    13681 gcactatttg tgattgccag taaattacca aacagaatat ggtacaaatc tgtcgtagtg
    13741 tttattaagc taataataat aatccccgaa gctaagaaag cggtaaaact aattccaata
    13801 gcggcgtcag gtttcgtttt actactagat gtgatataac cgataaaaat acttgcgatc
    13861 ataccagtta taagtgcgcc tacaaacatt ggaataccaa ataagaatga tagggcaaca
    13921 ccaggtaata ctgcgtgact cattgcatct cccattaatg aaagaccacg taatacaatt
    13981 aaactaccaa ctgtaccaca aactatccct acaataattg aagttatcaa tgctcgattc
    14041 aagaattgat atgtaaataa atgttcgaca aactctaaca tgttatattg ctcctttgac
    14101 tagggtcact acagtcagtg ctactcataa atgtttcgtt taagcgagtg acactcatag
    14161 cctcttcact atcaccaaag tatcgtaatg tttgatttaa tagaataatg cgatcaaagt
    14221 attgctttgc ttttgataga tcatggtgga tgataagaat aagttttcct tgttgtttta
    14281 agttctcgat ttttgtcatg attaattttt cgctactaaa atcaattccg acaaacggct
    14341 catctagaaa ataaacttca ctttcggaca tcaatgctct tgctactagc acacgttgta
    14401 attgtccacc acttaattct gaaatttgtc gatgacgtaa agattctaat tctaaatcgc
    14461 ttaataactg tttgagttta tcccttgctg atttattagg tcgtctaaac catccaattt
    14521 ctttgtagca acctgataaa atcacttgtt ccacacttat aggaaaatct aaatcaatat
    14581 gtgctttttg tggaatatat gtaatatgtt gcagttgttg ttgtataggt ttgttatata
    14641 acaatttagt accggtagca ttaaattcac caattaaaga cttgataagg gaagatttac
    14701 cagcaccatt cgggcccatg ataccaatta tttcgccgcg tactggtatc gataaggaaa
    14761 tgtttttaag tacatgctta ttacctaaaa acagatttaa atcttttgtt tctaacaaac
    14821 gtttatacct cctaattaaa agtttaggct aacctaatta attgtataat aaactgagaa
    14881 tatttatcat gtcaagtaaa ttcgtgatat aatatagaca atgtatgtga ggtgaaagta
    14941 tgttaactga agaaaaagag gactatttaa aggcaatcct tacgaataat ggcgataaaa
    15001 actttgtgac aaataaaatc ttatctcaat ttttaaatat taagcctcca tctgtaagtg
    15061 aaatggtagg acgtcttgaa aaagcaggct atgttgaaac aaaaccatac aaaggtgtta
    15121 gattaacaga ggatggttta acgcatacgc ttgatatcat taagagacat cgactattag
    15181 aattattttt aatagaaata ttgaaatata attgggaaga agtacatcaa gaagcagaaa
    15241 ttttagaaca tcgaatttca gatttatttg ttgaaaggct ggatagcctg ttaaatttcc
    15301 cagaaacttg cccgcacggc ggtgtgattc ctagaaataa tgaatataaa gagaaatata
    15361 taacaacgat tttgaattat gaacctggtg atatcgttac aatcaaacgt gtgagagata
    15421 agaccgattt gctaatatat ttgtctagta aagatatttc tattggtaat gaagtggaaa
    15481 ttgtatcgaa agatgaaatg aataaagtaa ttatcattaa acgtaatgat aatgtaatta
    15541 ttgtcagtta cgaaaatgca atgaacatgt ttgctgaaaa ataaaataaa gaagccataa
    15601 agatatccat gattgaactg ataaagacat atggataatt gctttaggct tcttttttat
    15661 tagttaattt atcaagtgag tatatttgag taaaatattc actgcataaa gattgaagat
    15721 aatccagatt gtactataaa tgaagatagg tacatgactg agttctttaa gtgcactacc
    15781 atcccactgt ggactcggac gctggaaagt caatttagca atcgtccaac tagattgtag
    15841 aacttcgcct aataatacac ctaaaatata ttgataactc attgtgacaa gtagttgaat
    15901 ttctactata ttttcatctt ttaatataaa atacaacatg atagaaatta aagttataac
    15961 aacaatgggt gagccttttc tagatgttaa aattaaaaaa taaataaata tcaataaata
    16021 ggtaaatata aagaaactag gtatctgata atggctcgac gctaaaccta tcaataacat
    16081 aataggtggc attaaataac caccaatcgt tgtaagccat tggcctgcta gatgtctaga
    16141 ttgtgtaatt gcgaatcctt gttgtaatgt ctgttgtcgc tctcgtggac ttgttacaat
    16201 gactaaatct tttgcacggc caccagcgag tttattaaac agtacatgac caaattcatg
    16261 tgttaaaaca gggatatagt ttaaaatgac atctaaatag ttcaaaacag gcttatgtct
    16321 atattgatga atagcaatat aacaagctgc aacaataacg ataatgtata tattaagttg
    16381 aattgtcgta ttaaaaaagt ttgataaata attcattgtt aacctcatat aagatattaa
    16441 tttaaagttt gcttagcact tattataaat gatattggca tcaatagcgt tagactttag
    16501 acttacctta gttaaactaa ttttaatttt tgaaaaggtg aatatgtgtt aaaa
//
LOCUS       DAMZLE010000052        16504 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMZLE010000052 DAMZLE010000000
VERSION     DAMZLE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.052"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1346
                     /gene="brnQ"
                     /locus_tag="POB92_001443"
     CDS             <1..1346
                     /gene="brnQ"
                     /locus_tag="POB92_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6578950.1"
                     /translation="LTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGF
                     LITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPRLATT
                     SFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLVLLAI
                     ILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATIKKLG
                     IENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYL
                     GNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLFANVG
                     LTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDGLKAS
                     PEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQA"
     gene            1599..2783
                     /gene="rocD"
                     /locus_tag="POB92_001444"
     CDS             1599..2783
                     /gene="rocD"
                     /locus_tag="POB92_001444"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDI6578951.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            2822..3853
                     /locus_tag="POB92_001445"
     CDS             2822..3853
                     /locus_tag="POB92_001445"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDI6578952.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            3865..5106
                     /gene="argJ"
                     /locus_tag="POB92_001446"
     CDS             3865..5106
                     /gene="argJ"
                     /locus_tag="POB92_001446"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDI6578953.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5122..5886
                     /gene="argB"
                     /locus_tag="POB92_001447"
     CDS             5122..5886
                     /gene="argB"
                     /locus_tag="POB92_001447"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI6578954.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            6156..6635
                     /locus_tag="POB92_001448"
     CDS             6156..6635
                     /locus_tag="POB92_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDI6578955.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(6963..7607)
                     /locus_tag="POB92_001449"
     CDS             complement(6963..7607)
                     /locus_tag="POB92_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDI6578956.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7620..14795)
                     /locus_tag="POB92_001450"
     CDS             complement(7620..14795)
                     /locus_tag="POB92_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDI6578957.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDLNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITHIDYMPLTTNDKVDTTRLPNPSTIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGVLEDSLSHRPIGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIARYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15242..16492)
                     /locus_tag="POB92_001451"
     CDS             complement(15242..16492)
                     /locus_tag="POB92_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6578958.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
BASE COUNT         5330 a         3131 c         2584 g         5459 t
ORIGIN      
        1 aactaacatt taaagaaaac atgtttatag gttctatgtt atttggttta ttctttggtg
       61 ccggcaatct tatcttccca atacacttgg gtcaagctgc tggttctaac gtttttatcg
      121 ctaacttagg atttttaatt acagcaattg gcttaccatt tctaggtatc attgctattg
      181 gcatttcaaa gacatctggt ttatttgaaa ttgcatcgcg tgttaataaa acatatgctt
      241 acattttcac gattgcctta tatctagtta tcggaccatt tttcgcctta cctagactgg
      301 caacgacatc atttgaaatt gcattttcgc catttttatc accaaagcaa atcactttat
      361 atttatttat ttttagcttc gtcttctttg tgattgcatg gttttttgcg agaaagccat
      421 caagaatttt agaatatatc ggtaaatttt taaatccggt attcttagta ttattagcaa
      481 ttattttatt atttgctttt atccatccat taggtggcat atctgatgca cctattagta
      541 aacaatatca atcaaatgcc ttatttaacg gctttttaga tggatacaat accttagatg
      601 cgctagcgtc attggcattt ggtattatca ttgttgcaac gattaaaaag ttaggtatcg
      661 aaaatccaac tgatatcgct aaagaaacaa ttaagtctgg tactatcagt atcattatga
      721 tggggatcat ttatacccta ctagcaatca tgggtacatt aagtattggt catttcaaac
      781 ttagtgaaaa tggtggtatt gccttagcgc aaattactca atactactta ggtaactacg
      841 gtatcgtcct gttgtcactt atcgttatgg ttgcttgttt aaaaacagcc atcggtttga
      901 ttacggcatt ttcagaaaca ttcgaacacc ttttccctaa aatgaattac ctagcgattg
      961 caacagttgt aagctttatt tcgttcttat tcgcgaatgt tggtttaact aagattatta
     1021 tgtactcagt cccagtgtta atgttcttat atccattagc aattgccttg attgtactaa
     1081 cattatttag tagcaaattc catcattcaa aacttattta tcaatgtacc attttcttta
     1141 caatgattgc tgcattagta gatggattaa aagctagtcc agagttcatt tcaagcacat
     1201 cattctcaca aactttgatt aatttcagcc aaaaatattt accattatca gacattggta
     1261 tgggctgggt tgttctcagt ttgattggtt tcattatcgg cttcattatt tataaaatta
     1321 agcatcgtaa aattccacaa gcataatact atgccacagt catatgttaa acatatgctt
     1381 gtggcatttt ttattcatac tacattaaac tgcaatcgta tacatacata tcaatgatta
     1441 tccacaaaaa atattagtac tttcatttta caaatcacat taatacaaac acaaccttat
     1501 ctttatatta ttaaatttac atttgacact tatattgaac aactgtaata tattaatatt
     1561 aattctttaa aatgtataaa tataaaggag ggagaccgat gaattcaatc attgaattaa
     1621 ctgattatta tagctctaat aattatgcac cacttaagct tgtcatttct aaaggtaaag
     1681 gtgtcaaagt ttgggatact gatggcaaac aatatataga ttgcatttcg ggtttttcag
     1741 ttgcaaacca aggccattgt catccaacaa ttgttaaagc gatgacagaa caagcttcaa
     1801 agttgtctat catttcacgt gtcctttata gtgacaatct cgggaaatgg gaagaaaaaa
     1861 tttgtcatct tgctaagaaa gacaaagtac tcccccttaa ctctggtact gaagctgttg
     1921 aagcagccat taaaattgct agaaaatggg gctctgaagt taaaggcatt actgacggac
     1981 aagttgaaat catcgctatg aataacaatt ttcacggtcg tacacttggc tcattatcac
     2041 tatctaacca cgacgcatat aaagcaggat ttcaccccct acttcaaggc actacaacag
     2101 tagattttgg agacattgaa caattaacac aagctatttc accgaataca gcagcaatta
     2161 ttttggaacc aattcaaggt gaaggtggcg ttaatatacc accgaaagga tatattcaag
     2221 ctgtgcgtca actatgtgat aaacatcaaa tattattgat tgcagatgaa attcaagttg
     2281 gtcttggtag aactgggaaa tggtttgcta tggaatggga gcaagtcgtt ccagacattt
     2341 atattttagg taaggcattg ggtggcggct tataccctgt atctgctgta cttgcaaata
     2401 atgatgtcat gcgtgttcta acaccaggta cacatggttc aacatttggt ggtaaccctt
     2461 tagccattgc aatatcgacg gcagcgcttg atgtacttaa agatgaacaa ctggttgaac
     2521 gatcagaacg cttaggttca tttttattaa aagcgttgct acaacttaaa catcctagta
     2581 ttaaagaaat tagaggtcgt ggtttattta taggcataga gcttaacaca gatgctgcac
     2641 cttttgtgga tcaactgatt caacgtggaa tcttatgcaa agacacgcat cgtactatca
     2701 ttcgattgtc tccacctcta gtcattgata aagaggaaat ccatcaaatt gttgcagctt
     2761 ttcaagacgt ttttaaaaat taacaattaa tcatttatat atgacatagg agggattcat
     2821 gatgattaaa gtaggtatcg ttggcggtag cggttatggc gcaattgaat taattcgatt
     2881 gttacaaaca catcctcatg taacgattgc acacatctac tcacattcaa aagtagatga
     2941 accgttgaaa ttaacatttc cacatttaca acatattatg caacatttcg aagcacttac
     3001 agtggacaat aatgactgtg atgtaatttt ctttgcgaca ccagcacccg taagtaaaac
     3061 atgtatccct cccttagtag aaaaaggtat tcatgttatc gatttatctg gcgcatttag
     3121 aattaagaat cgtgaaatat atgaagcata ttacaaagaa actgctgcag cacaagatga
     3181 tttgaatcat gctatttaca gcatttcaga atggcaatcg tttgataaca atggaacgaa
     3241 gctcatttct aatcctggct gtttccctac agcaacatta ttagcattac atccacttat
     3301 tagcgaaaaa atagtagatt tgtcatctat tattattgat gctaagaccg gcgtgtcagg
     3361 tgctggtcgt tcattatcac aacgtgttca tttttcagaa atgaatgaaa atctaagcgc
     3421 ttatgcaatc ggaaaccata aacacaaacc ggaaatcgag caatatttat ctatcattgc
     3481 gggtcaagat gtatcagtca tatttacacc acatctcgta ccaatgacac gaggtatttt
     3541 atcaacaata tatgtcaaat tatcatctga atatacgact gaatcattac ataaattaat
     3601 gacctcttat tatgctaatc agccatttgt cagaattaga gatattggga cttttccaac
     3661 cacaaaagaa gtactcggta gtaactactg cgatatcggc atctatgtag atgaaacaac
     3721 gcaaacagca attttagtat cagtgattga taaccttgtc aaaggcgcaa gtgggcaagc
     3781 cattcaaaat ttaaatatat tatatgattt tgaagtgacg actggcctaa atcaatcacc
     3841 agtttatcca taaggggtgt tagaatgaaa catcaagaaa cgacatcaca acaatataac
     3901 ttttcaatta ttaaacatgg cgatatcagt acacctcaag gcttcacggc tggtggtatg
     3961 cacatcggtt tacgcgctaa caaaaaagac tttgggtgga tttactcatc gtctttggca
     4021 agtgcagctg ccgtatatac tttaaatcag tttaaagctg caccacttat tgtcactgaa
     4081 gacactttac aaaagtctaa aggaaaatta caagcacttg ttgttaattc agctaatgca
     4141 aattcttgta ccggtcaaca aggcatagat gatgcacgac aaacacaaac atgggttgct
     4201 caacaacttc aaataccatc tgagcatgtt gctgttgctt caactggggt cattggtgaa
     4261 tatttgccta tggataaaat taagactggg accgaacata ttaaggatgc taattttgca
     4321 acgccaggtg cgtttaacga ggcaatttta acaactgata cctgtacaaa acatatcgct
     4381 gtatcactaa aaatcgatgg taaaaccgtt acaattggtg gtagcaccaa aggttcaggt
     4441 atgattcacc caaatatggc taccatgctt gcttttataa caaccgatgc atcgattgaa
     4501 tcgaatacac ttcatcaatt attaaaatct tcgactgacc atacatttaa tatgattact
     4561 gttgatggcg atacaagtac aaatgacatg gtattagtca tggcaaatca ccaagttgaa
     4621 caccaaatac ttagtcaaga ccatccacaa tgggaaacat ttgttgatgc attcaatttt
     4681 gtctgtacat ttttagctaa agctatagcc agagatggcg aaggcgcaac aaagttaatc
     4741 agcgtcaatg tgtcaggcgc taaatcaata tcagatgcaa gaaaaatcgg taaaactatc
     4801 gtaagttcaa atctagtaaa atcagctatt tttggcgaag atgccaattt tggtcgaatc
     4861 attacagcta ttggctacag cggatgtgaa attgatccta actgcacata tgttcaactg
     4921 aaccaaatac ctgtcgttga taaaggtatg gctgtactat ttgatgagca agctatgtcg
     4981 aatacattaa ctcatgaaaa tgtcacaatt gacgttcagc ttggtttagg taacgctgca
     5041 gcgactgcat acggttgtga tttatcctat gattatgtgc gtatcaacgc atcatatcga
     5101 acataaggtg gtgttggtta gatgaaattt attgtcatta aaattggtgg cagtacactt
     5161 agtgacatgc atccatcaat tattaacaac attaagcatt tacgatcaaa caacatctac
     5221 cccattatcg ttcatggcgg tggcccattt attaatgaag cattatcaaa ccagcaaatc
     5281 gagccacact ttgttaatgg cctaagagtg actgataaag caaccatgac cattactaaa
     5341 cacacgctca ttgcagacgt taacactgca ttagtagctc aatttaacca gcaccaatgt
     5401 tctgcaatag gcttatgtgg tttggatgca cagctgtttg aaattacatc ttttgatcaa
     5461 caatatggat atgtcggtgt tccgaccgct ttaaataagg atgctttaca gtatttatgt
     5521 actaaatttg tacctatcat caattcgatt ggtttcaata accatgatgg agaattttac
     5581 aatattaatg ctgacacgct tgcctatttt attgcatcat cattaaaagc gcctatttat
     5641 gtattaagta atattgcagg tgtactcatc aatgatgttg ttatacctca attgccatta
     5701 gtcgatattc atcaatatat tgaacatggt gatatttatg gaggtatgat tcccaaagtg
     5761 ctagatgcca aaaatgcgat tgaaaatggc tgtcctaaag ttatcattgc atcaggaaac
     5821 aagccaaata tcattgaatc tatttacaat aatgattttg ttggcacaac aatccttaat
     5881 tcataactat gaaattaagg cctaacaagt tttgacacgc gagatgattc cagttcgatt
     5941 atccattgcg ctaaaacatt tatttaccgt tcatctcgtt aacaattttg aatacagtac
     6001 gatacaatat gagatgtaaa aaactaataa ccttttacaa atttgtttat caaaatattt
     6061 taagttctgc aaagcttttt attgtgatta ttttcacaaa atactataat gaggatagta
     6121 aatagagagg agtccttaag ttgacgaaac gacaaatggg tatattcatt tatgctggaa
     6181 ttatcggtgg cttgttatct ggaattgtaa aattaggttg ggaggtcatg tttccacctc
     6241 gcacaccaga acgtaatgca acgaacccac ctcaagagtt attgcaacaa ttaggattta
     6301 gtagtgagtt tacgcatcaa acatatacat tttcaaatat ggaattgcct tgggtaagct
     6361 ttattgtcca ctttagtttt tctatcgtca ttgcaattat ttactgcata ttagttaaaa
     6421 aatacgctta cttagcaatg ggacaaggtg ctgtttttgg tattgctatt tgggtattat
     6481 tccaccttat cattatgcca atcatgcata ctgtacctgc tgtgtgggat caaccattcc
     6541 aagagcatct gtcagaattc tttggccata tcgtctggat gatgacaatt gaattagtgc
     6601 gacaacattt tgtctatcgc tataaattaa attaatacac tgactaacat taacgtgagt
     6661 tttaaatcat cgtttgagta tgatgattga tgctcacgtt attttattaa ctgacatgat
     6721 atgattccag ccaacttacg tgagcattaa agtctcaaat gcgtcgtaac aaactattat
     6781 tttcggtaat ttcaatattg ctcagtatat ttttacctta tcacttactt taatctcgtc
     6841 atgattttga atgatgccat cgtgtattca cctttcattt ttccaataaa aaaacatcta
     6901 acagtaaaca tttaggcagt atagtttaaa atcactgcgc aatgatactg tcagacgtca
     6961 tattaactac tcaataactg aaatacagac acttttttat acccccaggg tgcctgtcct
     7021 aagaaacata cctgtaccat aaactgatca aaaataaatt gtttgaactt cacttcacgt
     7081 gattgataaa agtgtgattg tgtcatatca taaatgtcca atcctttgat taaaccttca
     7141 ccaatcaatt ttgtaaaact ttctttttgt gtccatattt gataaaaatc atttaaacta
     7201 catatttgat gtgcttcgtt tgtagagaaa cacgtcacta acgtacgcca gtctaaacgt
     7261 tgtgatatct tttcgatatc aataccaact ggttctttat cgacaacaca cacgatataa
     7321 ggataactat atgataagct cacatagatg ggctgtccat cacgattgtg ttgaacaata
     7381 tctgccttac ctcgtggcga aatgtgataa tgccattcat gtggtaataa acctgtgtca
     7441 tgttgaattc catattgcac taaaatatct cccaatctgt gcatgagttt atcttgattg
     7501 tatctatagt tgactgtacg cggtttttta tatgaccaac gactttgtga tattaattct
     7561 tcaatacttt tcaagttact ctgtaattgc attacaaata ctgtcataac tttccctact
     7621 tacttattga atattgtttt gatatattgt gcccaatgat acagccaatt gttagttatc
     7681 gttggccatt tttcactgat gtgattcatt atttttaatg ttaatgttgt atctatcatt
     7741 gctagttgtt gttcacggtc aacactagtt aatccaatcg tttcgtacat gtcttgtttc
     7801 tgtaaaattt cattaaatga ttcatcgctg acgagttcaa tttctttgcg cttaacgcat
     7861 tctaacaaag atttcaccgg cattttatta ggtgatagca catggtaaat gatttgtggt
     7921 gtgttgacct gtgctaatgc gacaatttgt cttgcagtcg tatccacaaa agaaaaatct
     7981 acaggcattt cagccatgct aaccccgata caatccagtt gtaacaaatc attcattacc
     8041 attgaaaaac ggttagtctt tatatttctc atatgccatc ttccattgta aggactcgtc
     8101 aaattaccaa cacgtacaat ccgaccatct aagccattat ttacagcttc taatactttt
     8161 aattcactat aaaatttgct ccgtgtatat ggtgatgtta gtagttgccc tttatagaca
     8221 tccgcttctg aaaatgtcac atcttctgtg tctatatcaa aataagttcc cacacttatc
     8281 gtagacacat atattaacct tgcatgatgt tgttgtgcca aacgtatgac atcaacagta
     8341 ccttgaacat ttactttttc aaattcatca tcatcaccaa agtgatctgt acgagcacct
     8401 gcatgaataa tcgtatccat gttttctggt aaaacaacat catccataca ctcgaaatca
     8461 ccaacaatga cttcaatgtt tgataacatc atttcaaccg tctcttctga aaaataatca
     8521 tttaaattcg tcatcaactt ataccttgct atttcctcat tatcagcacg tatgaaacaa
     8581 taaatgcgat gactgtatcc ttgtagtgct tcaatcagat aagcacctaa aaaccctgtc
     8641 gcgccagtca atagtgtatt tcctataggt cgatgactta gactatcctc taaaacaccc
     8701 aaattataac gagacataac aatcttttgt aattccgaaa gattatccgg taatgcaact
     8761 aatgattgtt gattttggtg catataatta acaatctgtc gcacggtttt atattggtat
     8821 aatgtctgca ttgaaatatg atggccaaat cgttttaaat gcgagacaac taacatcgcc
     8881 tctaatgagt taccaccaag ttcaaagaaa tcatcgtcaa caccgacatc attttgtttc
     8941 aatacctctc cgaatacatc aacaaatgtc tgctcaattt cattagatgg ttcgctatac
     9001 actttattag actgttgtat agttgatgga tttggcaaac gcgtagtatc caccttgtca
     9061 ttcgtggtta atggcatata atcgatatgc gttatagtct taggaatcat atacttaggc
     9121 agatgatcat ttaaatattg cttcaaatcc tgctccattt gttgctctcc gacataataa
     9181 gcattcaata tatcatgcgt atcaaagtga cttactgtaa caacacaatc agatatacca
     9241 cgaatagcta atattgcatt ttcaatttca tcaagttcaa tacggtaccc gttaactttc
     9301 acttgtttat ctattcttcc taaaaattca atttgaccat cagatgtata acgtgctaaa
     9361 tcaccactat gatacaactt tcctttacca aatggattat tttgccattt atcagccatt
     9421 aattctggac ggttaatata tcctatcgct aaactatcac ctgcaataca caactcgcct
     9481 ggcataccaa taccgcataa caaaccatct gacataatat acacttggat gttagataag
     9541 ggtttgccaa ttggaatcgt ctcaggtatc aaatcaccac attgatgtga ccaatacgat
     9601 gtgatgactg ttgactcaga tggtccatag gcattgaaat acgtgccaca atgcttctca
     9661 atatatttaa caaaggatgc cgtactagtt gccccgcctg taatcaactt ttcaatataa
     9721 aagtcttcca taacactaca catctgtaac ggaatcgacg caaccgtcac acgatgctta
     9781 ttaatgagtt gttgtaactg ttctggatta acacgttcct ctctatctgg aatcacaagc
     9841 gtatgaccat ttaacaaaca acaataaatc tccataactg atgcatcaaa aacaatattt
     9901 gcatgttgca aaaatacttc attgtcgcct aattgcaatt cagttgacca tgcatgcact
     9961 aaattcaaca aatttcgttg tcgtatggca acccctttag gcatcccggt cgtaccagat
    10021 gtgtaaatag catacatctc attatctaac atcgctgtgt tttcaagttg attgccatgt
    10081 aaatcatcat attgttcatt ttcctttgat tcaacaaagc ctttagcatt ttccaattga
    10141 atgactggta atgtcgtttc aatttcaacg ccgtaagaca tgactgcagt tactttagca
    10201 tcctccaaaa ttgcaccttg tcgtttattc ggaaaatcaa tatcgatagg tatataagat
    10261 gcacctactt taactgtcgc caacatcgcc gcaatcattt caaaactacg ttctgtaaac
    10321 aaggcaaccc gttgaccatt gcccacacca tttgatagga gcatgtgcgc aatggcatcc
    10381 acatagttgc gtaatgtttc atacgtcatt gtcaaatcat tcatgactag cgcaacatga
    10441 ttaccttgtc gtgagacaac ttcattaaag taacttatga tagatttatt tcccgggaca
    10501 ttaagcattc gatcgttaac atgcgtattg acccaattta gaagttcctc cgtgccgttt
    10561 ggtatatcac aaatttgtag tgtatcttga tgcttcaaaa tataatcaat cataatcata
    10621 cattgattac ccatgtgacg aactgtttct gagtgatata aatcggtatt atactcgata
    10681 ttgattgtat agtcatcgcg atcttcttca atgatgaaag ataaatcaaa tttcgccgtc
    10741 actgatttgg gttgaatgtg tgttaattta ctatgcccaa aatgagcatg attcgtttca
    10801 ttgttttgta gtactaacat gacatcaaat aatggattcc gtgaggcatc atgtgattga
    10861 tctaagtcat ttactaaaca ttcgaatggg tattcttgat gctcgtatgc ctccaaactc
    10921 atttccttaa cctcttgtaa aaactgtgtc cacattttat caggtgacgg ttgccctcta
    10981 tataccaacg tattagcaaa catgcctagc atttgctccg tgcctttatg catacgcgca
    11041 ctcatcacac taccgacaac aacatcatct tttcgagcat atctacttaa caacgtcatg
    11101 accacactca taaagaacat aaaatcagta atttgatgct tttctacata cttttgaagt
    11161 agctgtctca tttgttgatt cattgtaaat gacatcattg ctccatttgt cgttttaata
    11221 tttggtctaa catagtctgt cggtaagctt aaaataggta cttcatcttt gaattgagat
    11281 aaccaatatt gtctatgttt cgtcatatca cgatgcgaca tccactcact atagtcttta
    11341 tattgcaatt taagtggtaa caataattta tgttgataaa gtgcgttaag atcattcatt
    11401 aattgtatat tactcatacc gtcattaatg atatgatgcg tatctataaa gaggtatgca
    11461 tgtaagggac ttctaatgta tctcactcta atttgacttg gcttttccaa attaaaaggt
    11521 gctacaaatt ggcgcatgat ttcttgttca tccgtaaaat gcgtgttaac ttcttcaaag
    11581 tcaactgcaa catctgccac aatacgttgt cgaacctcat catctacaac aatatattgt
    11641 gttcgtaaaa tctcatgtcg cgctatcaaa cgctgcactg cttgtcgcaa ttgagctaca
    11701 ttaagttctg atgataaccg ccataaaaaa ggtacgttat acaccgtatc tttatggttt
    11761 gatttccata ataaatacat acgcttttgt gcagagctca gcacataatc atctttaact
    11821 atagtttctg gaatcacttc atagttttgt tcttgaacct tagcaatcgc ttgtgctagt
    11881 tcaaatacag ttggcttttg taataaatca ccaatttgta atcgtttccc agtagatgcc
    11941 tctatccgat tcaccactaa cgttgctttt aatgaatggc cacctaattc aaagaaatta
    12001 tcatgaatac ctacttgatt cacatgtaaa atatctgcaa aaatttggca tagcaagtgt
    12061 tcggtatctg tactcggtgc tacataggca tccgtatcga catagtccat gataggcaat
    12121 gccttcttat ctaatttccc attaatagta ataggaattt gctcaatatg catgaaatta
    12181 actggtatca tgtactccgg taaggtcata cgtaattgtg atttaatctt attatgtgat
    12241 aatgtatgca tcgcttcata ataagcaacg atatactgat cttgatcatg attttgaaca
    12301 ataacaactg ctttattaat accttgtata cgctcgagcg catgctcaac ctctgacaac
    12361 tcaatcctaa accctcgaat cttaacttgt ttgtcctttc gatataaata atctatgttg
    12421 ccatcgggta acaaacgaac gatatcacca cttctataca tcagctgatt tatatttgaa
    12481 tctttgataa atttatctgc tgtcaattct ggctgattta aataacctgc agctaaccca
    12541 aagccacttg tacataattc tccaggaata ccaacgccac accgacgctc gccttgcatg
    12601 atataaacat gagtacccag aatcggttta ccaataggaa tacgatttgg aactttgtta
    12661 ggtatattat acgtcgttgt aaatgttgta ttttcagttg gtccataacc attaataatt
    12721 tgaggatgct tcggtttttg attaagcaaa tccacccact tagcattcaa tacttctcca
    12781 ccaattaata aatactttaa cggtaccaat acttctattc gttcactagc aatctgatta
    12841 aataatgagg aggttaacca catagtatta acgtcatttt cattgattaa ttgttctacc
    12901 gctattggat ttaataattg ttctttttta gcaacaatca gctttccacc attgagcaat
    12961 gcaccatata tttcaaatgt tgcagcatca aaggctatag ttcctgataa caaaatcgtc
    13021 gtctcttcat ttaatggtac ataatgattt tgatggacca agcgaacaat acctcggtgc
    13081 ggaattagtg tccctttagg gttaccagtt gtccccgacg tgtaaataac ataagcatga
    13141 tcttctaacg tgttacattt agaaagatta tcaatatttt tccacgctat cttattcaaa
    13201 tcaatgtgat taatattttg tttaccattt tcatataaag cttggtacgt tattacaact
    13261 ttaggcgtta catcttttaa aatgtactcc tgacgatcac ttggatagtt cagatcaatt
    13321 ggcacgtaag ccccaccagc tttcaacaca cctatcatcg ctattatcat ctcaatactt
    13381 ttttcagcta tgacagcgac acgatcatta ggttcaacac catactggtt tctcaaacgg
    13441 tgtgctaaat cattcgcgcg tgcattcaat gtttgatatg ttataaacac tccgtcaaat
    13501 tgcacagcga catgattcgg cgttgcttca acttgttgct caaataaggt aacaactgtt
    13561 tgcgcatcat ctatctcagg caaacttaaa ttgatatcgt catataattg aatatcacgt
    13621 tctgtcatca aattaagttc atctacagtt gttcgtttat ttccattttc ttcagtaatt
    13681 tgcaaataaa tatttcgaac taagtcactc agcgtctcga ttgagagcaa atcatatgct
    13741 gcactgttat atataatttt gaacccgtgt tgatgtggaa aaatttcaat atccgctaaa
    13801 gatgtatgtg catcatgtat ttgatgtaca tcctcaataa catcattaca acaagacatc
    13861 atatgatgat aacaatgaaa aatagtctct agtgaaagcg aagacttcgc gcactgtaat
    13921 tgcgacatat tttgcaacac acatttatta aaatctgttg taaaacgttg acatacatct
    13981 tttgcatcga ttgttaacgt taacggcaca atatttccgt gtaaatcatt tggtaaatgt
    14041 gatggtacat gtatacctaa tgtgacatca tgttgttgac tcattatatg attagctaaa
    14101 tacacactaa cagccaacga tgccatatct attgatgtca tatcatcaat caaatacgtt
    14161 tgatataaag cttgttcaaa tggatgctta attggaaaat aactatcaat atggatgtca
    14221 gagttattct ctaaccgaaa atagtttgag tctaatgcta tatgcgatgc atcttgattg
    14281 tctttatcat catttctatt tatatgtgca tgctgtcgag tattgttaat aacagtattg
    14341 ccacgatatg cattgcataa atcatcaaga aaaatatcaa tttgactatc atcgaaaatg
    14401 gacacatgaa aatctaatag tatatatgca gcatcagcga actgaaacaa tttaactttg
    14461 aataaaggtg aatcattaaa atggtaagta cttaattctt gcttaaaaaa agcttctaaa
    14521 tcatagtttg cggaagaaga tggaacttgt tttatctcaa taaaaggcag aaattcatga
    14581 agtatcattg ttaaattgtc atcggtagta acatcaaaaa aatgtcttat agatgcatgt
    14641 tgttgcacaa ttgtcgataa tgcatacatc attttagtag cttcaacatt tttagcgagt
    14701 ttaacccaat acgcattacg gtgtgtcgtt gattctgtat tatttttgta tatacgaaaa
    14761 tattcctgtt gaaatctcaa attacccata atcataaaaa gtccttcttt catatcataa
    14821 tactcattac ttactgaaat tgcatgatga tatgataacc gacgaaatgt taattaactc
    14881 gttatgtaat gattaatata aaacaccatt cgcaacatat gagcgatata ttctacccta
    14941 aaatacatct tgtatcatcg ttacaattgg tatatttttc aatgtaaatt acatacatct
    15001 tcgataaata gcacactaca actcgttaat cactttctgt tgttcacatc tcattgcaaa
    15061 ctcaatattg ttgttacaaa atatccatga agcaagttta tattaaacaa acaactcgca
    15121 taaaacaatt gttatcctta aatttcaaca aattcttaat aaatttatct ctattttaat
    15181 tacgaccaaa ttaataggtt ttccatataa aaagatgcat aaaataaata tttaaataaa
    15241 ttcaatttgt attcacttgt ttttgtcccc caaatacacc agcaacaagc atgctagcac
    15301 caattgttaa aacgataaac atatacagtc ccatttgtaa tgacgttaag aaaacaccca
    15361 acacaatccc taacctagct agtgtttctg aaaaatgaat acctaatgca ttaactgcac
    15421 tatatgttcc tcttttagct ttaggaataa ttttaaagcg ttgttctgaa actataggcg
    15481 aataaataat ttcacctaca gtcgcaatta tcataaaaac aactaataag ccaaactgat
    15541 taagataggt tagaccacta tagccaacaa tataaatcag caaaccagtg attaaagctt
    15601 ttttaaaatc tattttcaat acaactttcg agattgaata cgtgagtaaa atgacgacga
    15661 ccgtattaat cattagcaag attgctaaca tcttagcacc tgtaatatca tatgaaccta
    15721 tacttattgt ttcaaactga tcctttagtc taatagcaat atatgaggag attgaaaatt
    15781 cacccatcat gatgatactg aaccccgaaa tcaataacat ataattacgg tctttcaaaa
    15841 ctaatttata actgcgaaat atattcatta tttgtaattt ttgataacga cttgcatgcc
    15901 tcttgtcatc actttgcttt acttgatttc ggtcttgagg taaccaaata tataaaataa
    15961 agagtacaat taaaaatata caagctgcta ttaagaaaag tagtaacata ctgtagccat
    16021 acatcaagcc acctaacaat gccccaatag ctaccgataa gttcgtcatc caatagctaa
    16081 tcttgtaaat ataatgttcc acgtcttcgg taattgcatc cataattaat gtgtccataa
    16141 ctggaaattg taatccccaa acgattgtaa atatggcata tgcaacacaa aaaccaataa
    16201 tttgccacaa ttgatgtgac ccaaatacgc ccatgaacac aagcattatc accatcgtcg
    16261 cttgataaat aagtactagc aactttttcg gaaaaatctc aataaggtaa ccagatataa
    16321 tggacaatgg aaattttaga accactaaac caacaagata tataccgaca attgattgac
    16381 ttaacatatc tgttaaatat agtgctataa acggtataaa tgctgtcgta ataattagct
    16441 gtaaaaaatt gctaatcaat cgtactttca aagtcgtact taagcgtttc attaataact
    16501 acac
//
LOCUS       DAMZLE010000053        16050 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMZLE010000053 DAMZLE010000000
VERSION     DAMZLE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.053"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(141..410)
                     /locus_tag="POB92_001452"
     CDS             complement(141..410)
                     /locus_tag="POB92_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578959.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            724..1410
                     /gene="mtnN"
                     /locus_tag="POB92_001453"
     CDS             724..1410
                     /gene="mtnN"
                     /locus_tag="POB92_001453"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI6578960.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            1430..1957
                     /locus_tag="POB92_001454"
     CDS             1430..1957
                     /locus_tag="POB92_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDI6578961.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1958..3058
                     /gene="yqeH"
                     /locus_tag="POB92_001455"
     CDS             1958..3058
                     /gene="yqeH"
                     /locus_tag="POB92_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDI6578962.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            3072..3878
                     /gene="aroE"
                     /locus_tag="POB92_001456"
     CDS             3072..3878
                     /gene="aroE"
                     /locus_tag="POB92_001456"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI6578963.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDDKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3882..4172
                     /gene="yhbY"
                     /locus_tag="POB92_001457"
     CDS             3882..4172
                     /gene="yhbY"
                     /locus_tag="POB92_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI6578964.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            4175..4744
                     /gene="nadD"
                     /locus_tag="POB92_001458"
     CDS             4175..4744
                     /gene="nadD"
                     /locus_tag="POB92_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDI6578965.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4734..5318
                     /gene="yqeK"
                     /locus_tag="POB92_001459"
     CDS             4734..5318
                     /gene="yqeK"
                     /locus_tag="POB92_001459"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDI6578966.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            5319..5672
                     /gene="rsfS"
                     /locus_tag="POB92_001460"
     CDS             5319..5672
                     /gene="rsfS"
                     /locus_tag="POB92_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI6578967.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            5675..6391
                     /locus_tag="POB92_001461"
     CDS             5675..6391
                     /locus_tag="POB92_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6578968.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            6431..7117
                     /locus_tag="POB92_001462"
     CDS             6431..7117
                     /locus_tag="POB92_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDI6578969.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTI"
     gene            7209..7670
                     /locus_tag="POB92_001463"
     CDS             7209..7670
                     /locus_tag="POB92_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDI6578970.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            7675..9876
                     /locus_tag="POB92_001464"
     CDS             7675..9876
                     /locus_tag="POB92_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDI6578971.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYKPLKRLNLPLFVIKGITIIVLALFAQYTNYAPIAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVTKFNDWIFIIFIISVYYIFWLLAKRKYILVTVW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIEIYNKGYSSNTL
                     MLLSKLSHKYNIKLIDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            9933..10907
                     /gene="holA"
                     /locus_tag="POB92_001465"
     CDS             9933..10907
                     /gene="holA"
                     /locus_tag="POB92_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI6578972.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVNQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(10952..11203)
                     /locus_tag="POB92_001466"
     CDS             complement(10952..11203)
                     /locus_tag="POB92_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI6578973.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            11549..13372
                     /gene="lepA"
                     /locus_tag="POB92_001467"
     CDS             11549..13372
                     /gene="lepA"
                     /locus_tag="POB92_001467"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI6578974.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            13996..15120
                     /locus_tag="POB92_001468"
     CDS             13996..15120
                     /locus_tag="POB92_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI6578975.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            15221..>16050
                     /gene="hrcA"
                     /locus_tag="POB92_001469"
     CDS             15221..>16050
                     /gene="hrcA"
                     /locus_tag="POB92_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDI6578976.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     G"
BASE COUNT         5919 a         2047 c         2914 g         5170 t
ORIGIN      
        1 tgttacaaat aaaaacagca gtaaggatga taacacttag aaaagtctta ctgctgtttt
       61 ctattagaat tatctttaat acacacagga attcgatacg aaacaaatat gattaactgt
      121 gtaattacaa aataaaacta ctatagtaaa aagaagataa agacgatgat aataaatgca
      181 tcgaatataa ttttcttgat gtttttatca acaacatcag gtaaggacat aagtataaat
      241 cctagaatta gagtgtagcg acatatgttc ataattgttg ctgtaaactc gatattaaat
      301 gaaaacttta aaaatatact tataaataaa atgacaaacg acgcaattgc aataaaactg
      361 ccgtatgtag ctatctttgt tacgagacta ttttgattat tttcagccat tgtaaaatcc
      421 tcttatttca tcattattat tttaaacata tcattttcct gttatttaat aaagtttctt
      481 gacacgattg gttgatgtaa ttgatatgat tagtttttga aaaatagaca aattagaagc
      541 atcgaattca ctgttatgag ttcaaaaata attatttatg ataccaagtt attgaaagag
      601 ctttttataa aaataaagca aaagttttat acagtaatga aaatcatgat acttgtcgtt
      661 gtagtgatta aaatgtgata aaataacaaa tgctaggatt ttttaaaaga ggtgaatcaa
      721 aggatgattg gtataattgg tgccatggaa gaagaagtaa caatattaaa aaataaatta
      781 acacaattaa gcgaaatttc agttgcacat gttaaatttt atactggcat tttaaaagat
      841 agagaagtag tgattaccca aagcggcatt ggaaaagtta atgctgcaat ttctacgaca
      901 ttattaatta ataagtttaa accggacgtc attattaata caggttctgc tggagcttta
      961 gatgaaagtt taaatgtagg tgacgttctt ataagtgatg atgtaaaata tcatgatgcg
     1021 gatgcaacag catttggtta tgaatatgga caaataccac agatgccggt agcatttcaa
     1081 tcaagtaaac ctttaataga aaaagtatct caagttgtac aacaacaaca attaacagct
     1141 aaagtaggct taattgtaag tggtgatagc tttatcggta gtgttgaaca acgtcaaaaa
     1201 attaaaaaag catttccaaa tgcgatggcg gttgaaatgg aagcaactgc aattgcacaa
     1261 acatgttatc aatttaatgt accatttgtt gtagttcgtg cagtttcaga cttagcaaat
     1321 ggagaagcgg aaatgagctt cgaagcattt ttagaaaaag cagctgtatc atcaagtcaa
     1381 actgttgaag cattagtgtc tcaattataa aaaggtaaag gtgagttaaa tgggtttagt
     1441 tcgcaagttt tttatgccga attcatatgt tcaatcaata tttcaaattg atttagacaa
     1501 gttagtggac aaaggcgtta aaggtattat tacagattta gataatacgc tagtaggttg
     1561 ggatgttaaa gaacctacag aacgtgttaa agcatggttt aaggaagcta atgaaaaagg
     1621 aatcactatt acaatcgtgt ctaataataa tgagtctcgt gttgctagtt ttagtcagca
     1681 tttagacatc gattttattt ttaaagcgag aaagccaatg gggaaagcgt ttgataaagc
     1741 aataactaag atgaatatca gaccagatca aactgttgtt ataggtgacc aaatgcttac
     1801 tgatgtattt ggtggtaatc gtcgaggtct atatacaatt atggttgttc cagttaaacg
     1861 aactgatggc tttattacta agtttaatag attaattgaa agacgattat tacgtcattt
     1921 cagtaaaaaa ggttatatca catgggagga aaattgattg tctgacattt taaaatgtat
     1981 cggttgtggt gcgccacttc aatctgaaga taaaaataaa cctggttttg taccagagca
     2041 taatatgttt cgtgatgacg tgatttgcag acgttgtttc cgcttgaaaa attataacga
     2101 agttcaagat gtaggattag aaagtgaaga ctttttaaaa ttattatcag gacttgcgga
     2161 taaaaagggt attgtcgtca atgtcgtgga tgtatttgac tttgaaggat catttattaa
     2221 tgcagttaaa cgtattgtcg gaaacaaaaa aatcatttta gcagcaaata aattggattt
     2281 attgccgaaa caaattaata aacgtcgagt taaagaatgg ttaaaacgaa cagcaagaaa
     2341 atatggtttg gaagctgacg atgtcgtatt aatttcagct gaaaaaggct ggggcataga
     2401 cgacttatta tcatcaattg cgaatattcg agaaaatgaa gatgtgtata ttgtagggac
     2461 aacgaatgtt gggaaatcta cattgattaa taaactgatt gaagctagtg ttggtgaaaa
     2521 agatgtagta acaacttcaa gattccctgg aacaacttta gatatgatag atattccttt
     2581 agatgaaaca tcatttatgt atgatacacc aggtattatt caagatcacc aaatgacgca
     2641 tttagttagt gaaaaagaat tgaaaattat tatgcctaag aaagaaataa aacaacgcgt
     2701 atatcaatta aatgaggcgc aaacattatt cttcggcggt ctagcgcgca tagattatgt
     2761 atcaggtggt aaacgtccgt tagtttgttt cttttctaat gacttgaata tacatcgtac
     2821 taaaacggag aaggccaatg atttatggcg taatcaactt ggcgatttat taacgccacc
     2881 tggaaatcca caaaattttg atcttaatga ggtaaaggct gttagacttg aaacaggcaa
     2941 agagaaacgc gatgttatga tctctggtct aggctttata actataggac caggagctaa
     3001 agtaatcgtt cgtgttccta aaaatgttga tgttgtatta agaaattcta ttttataagg
     3061 tgatttaaaa aatgaaattt gcagttatcg gaaatcctat ttcacattcc ttgtcgcccg
     3121 ttatgcatag agcaaatttt aattctttag gattagatga tacttatgaa gctttaaata
     3181 ttccaattga agattttcat ttaattaaag aaattatttc gaaaaaagaa ttagatggct
     3241 ttaatatcac aattcctcat aaagaacgta tcataccgta tttagatcat gttgatgaac
     3301 aagcgattaa tgcaggtgca gttaacactg ttttgataaa agatgacaag tggatagggt
     3361 ataatacaga tggtattggt tatgttaaag gattgcacag cgtttatcca gatttagaaa
     3421 atgcatacat tttaattttg ggcgcaggtg gtgcaagtaa aggtattgct tatgaattag
     3481 caaaatttgt aaagcccaaa ttaactgttg cgaatagaac gatggctcgt tttgaatctt
     3541 ggaatttaaa tataaaccaa atttcattag cagatgctga aaagtattta gctgaattcg
     3601 atatcgttat taatacaaca ccagcgggta tggctggaaa taacgaaagt attattaatt
     3661 taaagcatct ttctcccaat actttaatga gtgatattgt ttatataccg tataaaacac
     3721 ctattttaga ggaagcagag cgcaagggaa accatattta taatggctta gatatgtttg
     3781 tttaccaagg tgcggaaagc tttaaaattt ggactaataa agatgctgat attaattcta
     3841 tgaaaacagc agttttacaa caattaaaag gagaataaca tatgcttact ggcaaacaaa
     3901 aaagatactt aagaagttta gcacacaata ttgatccgat ttttcaaatt ggaaaaggcg
     3961 gtatcaacga aaatatgatt aaacaaatag atgatacgtt agaaaacaga gaattgatta
     4021 aagtacatgt actacaaaat aactttgatg ataaaaaaga attagctgaa acattaagcg
     4081 aagctacgcg tagtgaatta gtgcaagtga ttggatctat gatagtgatt tatagagaat
     4141 ctaaagaaaa taaagaaatt gaattgccat aataatgaaa aagatagtac tttacggcgg
     4201 tcagtttaac cctatccata ctgcacatat gatagtagct agcgaagtat ttcatgaatt
     4261 acagccagat gaattttatt ttttacctag ttttatgtct ccattgaaaa agcacaataa
     4321 ttttatagat gttcagcaca gattaacaat gatacagatg attatcgacg agcttggttt
     4381 tggagatatt tgcgacgatg aaattaaacg tggtggtcag agttatacct atgacacgat
     4441 caaggcattc aaggagcaac acaaagacag tgagttgtac tttgttattg ggacggatca
     4501 gtataaccaa ctagagaaat ggtatcaaat tgaatactta aaagaaatgg ttacttttgt
     4561 agttgtaaat cgagacaaaa atagtcaaaa tgttgaaaat gctatgattg caattcagat
     4621 acctagggta gatataagtt cgacaatgat tcgacaaaga gttagtgaag ggaaatctat
     4681 ccaagttctt gttcctaaat ccgttgaaaa ctatattaag ggggaaggat tatatgaaca
     4741 ttgaaaaagc aaaacggctt gcaaaagaga aactgccaga gaaacgttat aatcattctt
     4801 taagagttgc agaaacagct attaaattgg cagaaattta tgatggagac actagtaaag
     4861 tagaattagc aggtgtatta catgatttct gtaaatatga tgatttaggt aaaatgtatc
     4921 aaattgttcg acaatacgaa ttaggtaatg atctactgag ttatggtagt gaaatattgc
     4981 atggccctgt gtgtgcagca atcatggaac atgaatatgg tatcaatgat gaggaagtat
     5041 taatggctat caaataccat actactggac gtcaacaaat gacaaaaact gaaaaactga
     5101 tttttattgc agattacatc gaacctggaa gaacaatccc aggagttgat gatattcgag
     5161 atatggcata caatcaaggt agtttagata agacaattta tgaaatttct aaacgcacag
     5221 tactattttt aatacagaaa gatattacgg tatataataa gacgattgac tgtttaaatt
     5281 attataacta tagtgacgaa agaataaagg atgattaaat gaattcacaa gaattattag
     5341 caattgctgt ggatgcaatt gacaataaaa aaggcgaaga tacgatttct ttagaaatga
     5401 aaggtatcag cgatatgaca gattattttg ttgtaacgca cggaaataat gaacgacaag
     5461 ttcaagcgat tgctagagcg gtgaaagaag tagccaatga acaaaatata gaagtaaaac
     5521 gtatggaagg atacaatgaa gcgcgttgga tattaattga cttagctgat gttgtggtac
     5581 atgttttcca taaagacgaa agaaattatt ataatattga aaagttatat caagatgcac
     5641 cattagaatc atatagtcag gttgcgtatt aattatgtcg caatatgcag aaatgagcct
     5701 agtgtacgat caattgactc aagatcaacc atatgaaaaa tggtttgaaa ttgtaaaaaa
     5761 tcactgcaaa gatgaatcaa atattttaga tattggatgc ggtactggta gtttaacagt
     5821 tcaattagaa gctttaggta atgttactgg tatggattta agtgttgata tgttgactgt
     5881 tgcagctcaa aaatcagcaa atgtaaattg gcttgaagga gatatgacat cttttgattt
     5941 gcaacaacaa tttgatatca tcactatttt ttgtgactct ctaaattatt tgcaagatga
     6001 aactgcagtg attgaaacat tcatcaatgt ttatcatcat ctgactgatt caggtgtatt
     6061 tatttttgat gtacatactg tttataaaat gatgactttg tttaataatc aaagttatat
     6121 tgatgataaa ggggacattt ttttagcatg ggatgctgtg caaggagatt tacctttaag
     6181 tgtttatcat gatatgacat ttttcattcg acatgaagat gaaacatatt caagatttga
     6241 tgagtctcat tttcagagaa catttgatga aaagacatat ttgtcttggt tagctcaagt
     6301 tggttttaaa catgttgaaa catttactga ttttaatata gatgaacata atgaagatgc
     6361 agaaagattg tttttcattg cgaaaaaata aaagtaaaac cactcgctca gacgtgtata
     6421 aagttgagga gtggttttat tgtatcaatt tttattacgc tataaagatt ttttaactca
     6481 atggaagtta tatattataa gtgctgttgt tttaattatg gtattaattg gttttatatt
     6541 ctggagacaa gatgattata cttcaagaaa ttttgaaaat aaagataatg ctctgaaaca
     6601 aagcactagt gaaaatagta gtttgtccaa agtagaagat gtccaggtca aagatggaga
     6661 taattccaaa aataagggtc ctgtatatgt cgatgtaaaa ggtgctgtta aacatcctaa
     6721 tgtttataaa atgacatcta aggatagagt agttgattta cttgataaag cacaattatt
     6781 ggaggatgca gatgtaagtc aaattaattt gtctgaaaaa ttaacagatc aaaaaatgat
     6841 tttcatacct cataaaggac aaaagaatgt tgaaccacaa attggagtaa acagtgtgca
     6901 tgtaaaaaat gggaacacaa ataatactaa agtaaattta aataccgcat ctgtatcaga
     6961 attgatgtct gttcctggag tagggcaagc taaagctaat gcaattgttg aatatcgcaa
     7021 ccaacaaggt gcatttcaag aaattgacga tttgaaaaaa gtaaaaggtt ttggaagtaa
     7081 aacttttgat aaactgaaat cttatttcac gatataatgg attattttaa ttgcaaattt
     7141 tattttttgt gattaatctt atattaatag aaaatgttac taaattttaa gtatggaagg
     7201 tgaacatctt ggaaagaatc aaatgggaag aatattttat ggcacaaagt catttgctag
     7261 cattacgttc aacttgtcaa agattatctg taggtgcaac gattgttaag gataatcgta
     7321 ttattgctgg tggttataat ggctctgtag ctggcgaggt gcattgtata gatgaaggat
     7381 gtttaattga agatggacat tgtatcagaa cgatacatgc agaaatgaat gctttattac
     7441 aatgtgcaaa acaaggtgta tctactgaag gtgcaacaat ctatgttact cattttccat
     7501 gcctaaattg tacaaagtca attattcaag caggtataaa gcgtatctac tatgcagaag
     7561 attatcataa ccatgaatat gcaactaaat tactcaaaca atctggtatt gaatttaaaa
     7621 aaattccatt ttcaccagaa tatgttgcta aatatctgac taaaggttaa tgttttgctg
     7681 tatgtcgcgt tatcaatgat tgtaggagtg ctttggaatt ctagcaaagt gctctctaca
     7741 tttcttttca ttttactttt gtatattact tatcgtaaaa ataaaatcgt ttatgcccct
     7801 atttctctct ttttaatcat tttctcctca tggtatttac attattcaca acaagcaata
     7861 tttaattata tcaattatat tgaacgtaat tctcagttta atgagcgtgc tcaagtaatc
     7921 caaattcaac gtcaaggtag tgacacatat aaaggtaggt tgagtttaaa aaatgaaata
     7981 tatcctttct ttttaacaaa taaaaagaat tttgatttaa agaaaattga aagtcgtaat
     8041 tgtattgtta aaggacaatt caaagttaat gacaataagt ttgtaactct taaattacaa
     8101 agtatagttg tacaaagctg cctagaatcg aaccggtcta atttaattga gaaacataaa
     8161 cagtttataa tgaatcgaat ttatgattcg ggtattaagt ttccggatcg tattatggca
     8221 ttgattactg gtgacgtaaa agaaattaat gagcaattta aggaacgtgt taaagagata
     8281 ggtatatatc atttgctggc agttagtggc tcgcatatag ctgcaattgt attcttaatt
     8341 tacaaacctt taaaacgatt aaatttacct ttatttgtca ttaaaggaat tacaatcatt
     8401 gtattagctt tatttgctca atacacaaat tatgcaccta ttgctgtaag agctataata
     8461 atgacaactc ttgtactgct tattactaag caaattaaaa taaagggtat tcagctatta
     8521 gcatttgcat ttataattat gtttatttta aatccactag ttgtttatga tattggattt
     8581 caattttcat tcatcatttc attttttatt atgctacttt ttcctttttt acagcaattg
     8641 tcaaagttac aatcattatt cataattacg tttattgcac aattagcttc atttatcgtt
     8701 gccattccaa actttcatca acttcaatgg gtgggatttt tatctaattt gatttttgta
     8761 ccgtactatt cgattatatt gtttccgcta tctattttat tctttattac aagtcatttt
     8821 attgtgggat taacgccgct aaattacttg gttgacctaa gttttaattt tcatgactgg
     8881 ttactagacc tattcacaag aatcaagcaa tcacattttt ctgttaccaa gtttaatgat
     8941 tggatattta taatatttat aatttctgtt tattacatat tttggttatt ggctaaacgt
     9001 aaatatatat tggttacggt ttggactata attattctga cattattaat aacgtttcca
     9061 acaaattcac atcacaaaat tacaatgtta aatgtggggc agggagacag tattttatat
     9121 gaaggtggta agaaccaaaa tgtcttgatt gatacaggtg ggaaagtgat tgatgatact
     9181 aaacaaccta gttattcaat ttctaaatat catattttac caacgctaaa tgaaagaggg
     9241 ataaatgaat tagagtatct aattttaaca catccacaca atgaccatat tggtgaagtg
     9301 gaatatatta ttagtcatat taaaattaaa catatagaga tatacaataa gggatatagt
     9361 agtaatacat tgatgttatt atcgaaatta agccataagt acaacattaa acttatagat
     9421 gtaagacaag ttagtagttt taaacttgga gatagtagtt ttctattttt tgatagtttt
     9481 attccaaata gccgagataa aaatgagtat tcgattatta ctatgattac atatcaaaat
     9541 aaaaaagttt tattaatggg cgatgctagt aaaaataatg aatctttact actaaaaaaa
     9601 tataacttgc cggagattga tattttaaaa gtaggtcatc atgggagcaa gacaagtagt
     9661 tctaaagaat ttatagagat gattaagcct aaaataagtt tgatttcttc tgggaagaac
     9721 aatatgtatc atcttcctaa tatagaagtt gttaaacgat tgcaaaggat tcgcagtcgc
     9781 atttacaata gtcaacaaaa cggtcaagtt acaattgact tagatgataa tttaaaagtt
     9841 gattcaaact cttatggaaa tgcaagtggt ttatagatac agaaacatga tataatctat
     9901 tgatgtgtca aacgaatgaa agggtgtaat gaatgagcga caatattgta gctatttatg
     9961 gagatgtgcc tgaattggtt gaaaaacaaa gtgcagaaat tatatcacaa tttttgaaaa
    10021 gtgatagaga tgactttaac tttgtgaaat ataatttata cgaaacagag attgcaccaa
    10081 ttgttgaaga aacattaaca ttgcctttct tttcagataa aaaagcaatt ttggttaaaa
    10141 atgcatatat atttacaggt gaaaaagcgc caaaagatat ggctcataat gtaaatcaat
    10201 taatagaatt tattgaaaaa tatgatggcg aaaatttgat tgtctttgag atatatcaaa
    10261 ataaacttga tgaaagaaaa aagttaacta aaactctaaa aaagcatgca aggcttaaaa
    10321 aaatagagca aatgtctgaa gaagaaataa aaaaatggat tcaaagtaaa ttaaatgaga
    10381 atttcaaaga tatcaaaaga gatgcattag atttatttat tgagttgaca ggtattaact
    10441 ttaatattgt ctcacaagag atagaaaagt tgattttatt tttaggcgat agaccaacaa
    10501 ttaataagca ggatgttaac caaattatta atagaagttt agagcaaaat gtatttttac
    10561 tgactgaata cattcagaaa agaaagaaag aacaagcaat tcatttagta aaagatttaa
    10621 taactatgaa agaagaacct attaaattac ttgcactaat cacaagtaat taccgattat
    10681 tttatcaatg taagattttg agtcaaaaag gatatagtgg acagcaaatt gctaaaacaa
    10741 taggcgtaca tccatacaga gtaaaattag cgttaggaca agtaagacat tatcaacttg
    10801 atgaattatt gaatattata gatgcttgtg cggaaactga ttataaactt aaatcatcat
    10861 atatggataa acagttaata ctggaattat tcattctatc tttataaaaa ataaaagttc
    10921 aagcatgcgc ttgaactttt atttaaaaag attatttatt tgcagtcatt aattgtgatt
    10981 taatacggtc agctttgttt gaatgtatta aattactttg agcagctttg tctactaact
    11041 taactgctaa gcttactaat tcatttttat tatcagcgtt atttgaaaca gctgttttag
    11101 cgtttttaac tgctgtacgc attgcactct tttgtgaaat gttgcgtgct tcagcttttt
    11161 cagttgtttt tacacgttta attgcagatt tgatatttgc catttctgtc acctcctaaa
    11221 agtgagttca tcttatcaaa attttatttg attacaacaa gaaatatttt aacaaaagtc
    11281 taaccgttgt gcaatcattt atttgatggg gttagcttaa aattttgcag atttaataaa
    11341 ttgtgttgga aacacaatca atacgtttta taattataag tacgtaatta acatattaac
    11401 atatcgcaag tatgtattta aataaagatt gttataattt caaagttcat ccaagattat
    11461 ggcgtttgca tttacctatt aaaaacgtta ttatatcaaa gatgcgaaag ataatacggg
    11521 tttattttat gaaagtgaga aggataaaat ggataatgag caacgcttaa aaagaagaga
    11581 gaatataagg aatttctcga ttatagcaca tattgaccac ggaaaatcta cattggctga
    11641 tagaatttta gaaaatacca aatcagttga aacaagagat atgcaagatc agttactaga
    11701 ttcaatggat ttagaaagag aacgtggtat tacaatcaaa ttaaacgcag ttcgtttaaa
    11761 gtacgaagct aaagatggaa atacttatac attccattta atcgatacgc ctggacacgt
    11821 cgattttaca tatgaagtgt cacgttcttt ggcagcttgt gagggcgcga ttttagtagt
    11881 agatgcggct caaggtatcg aagcacaaac attagcaaat gtttatttag cattagataa
    11941 tgagttagag ttattgcctg ttattaacaa aattgattta cctgctgcag aacctgaacg
    12001 cgtgaaacaa gaaattgaag atatgatagg tttagaccaa gacgatgttg ttttagcaag
    12061 tgctaaatct aacattggaa ttgaagagat actagagaaa atagttgaag ttgtgccagc
    12121 tccagatggc gacccagaag caccactaaa agcgttaata tttgattctg agtatgatcc
    12181 atatagaggg gtaatttcat cgataagaat tgtagacggt gttgttaaag ccggagataa
    12241 aattcgaatg atggcgactg gtaaagagtt cgaagtaaca gaagttggaa ttaatacacc
    12301 taagcagctt ccagttgatg aattaacagt tggtgatgtt ggttatatta ttgcaagtat
    12361 taaaaatgtt gatgattcta gggttggtga caccatcaca ttagctagta gacctgcatc
    12421 agaaccattg caaggttata agaaaatgaa tccaatggta tattgcggac tgttcccaat
    12481 agataacaaa aattataatg atttaagaga agcattagaa aaattacaat tgaatgatgc
    12541 atcattagaa tttgagcctg aatcgtcaca agcattaggt tttggttata gaactggttt
    12601 cttaggtatg ttacacatgg aaataattca agaaagaatt gaaagagaat ttggcattga
    12661 attaattgca acagcaccat ctgtaattta tcaatgtatt ttaagggacg gttcagaagt
    12721 gacggttgat aacccagcac aaatgccaga tcgtgataaa attgataaaa tatttgagcc
    12781 atatgttcgt gcaactatga tggttccaaa tgactatgta ggtgcagtaa tggaattatg
    12841 tcaacgtaaa cgtggacaat ttataaatat ggactattta gatgatattc gtgtaaatat
    12901 tgtttatgaa ttacctttag ctgaagttgt atttgatttc ttcgatcaac ttaaatctaa
    12961 tactaaagga tatgcatcat ttgattatga attcatcgaa aataaagaaa gtaatttagt
    13021 taagatggat attttattaa acggtgataa agtggatgcg ctaagcttca tagttcatag
    13081 agattttgca tatgaacgtg gtaaagcatt agttgaaaaa cttaaaacgt taattccaag
    13141 acagcaattt gaagtacctg tacaggctgc aataggacaa aaaattgtag cgcgtacaaa
    13201 tattaaatca atgggtaaaa acgttttagc taaatgttat ggcggtgaca taagccgtaa
    13261 acgtaaatta cttgaaaaac aaaaagcagg taaagctaag atgaaagcag ttggtaatgt
    13321 tgaaattcca caggatgctt tcttggctgt attgaaaatg gatgacgaat aagtttttta
    13381 aaaatttatt aacaatttac aatgaataaa gtttaataac taaaaagagg gagcctagga
    13441 taaattaacg tcctgggctt tacaatgtta tattggcagc catcgacaga gttaaaaatg
    13501 agcttataac aatggggccc caacatagaa gctgacgaaa agtcagctta caataatgtg
    13561 caagttgggg tggggcccca acatagagaa tttcgaaaag aaattctaca ggcaatgcaa
    13621 gttggggtgg ggccccaaca tagagaaatt ggattgccaa tttctacaga caatgcaagt
    13681 tggggtggga caacgaaata aattttgcga aaatatcatt tctgtcccac tcccttatgc
    13741 atgagtttta ctcatgtaat tttattttta tggacatatt acatctggct aatgtgtaag
    13801 agccactaca taataaatca ttagtggttc tttattattt ctatctcact ccctctaaac
    13861 aagaataaat atcaaaatga atcgatatat tagacaacca ttgattaaac gttaaagtta
    13921 aaagtaagaa taattgcaga tagtccaaca ggatatagcc gattggataa acagtctgag
    13981 aagcggggca ttaaaatgac ggtacaaagt gcatatatac atattccatt ttgtgtaaga
    14041 atatgtacat attgtgattt caataaatat tttatacaga atcaacctgt agatgagtac
    14101 ttagatgcac taataacaga aatgtctaca gcaaaatata ggaacttaaa gaccatgtat
    14161 gtaggtggcg gcacaccaac ggccctttct attaatcagt tggaaagatt acttaaagca
    14221 atacgtgata cgtttacaat cacaggcgag tatacatttg aagcaaatcc tgatgagtta
    14281 actaaagaga aagtccaact attagaaaaa tatggagtaa acagaatttc aatgggcgtt
    14341 caaacattca agccggagtt attgtctgtt ttaggtagaa cgcacaatac tgaagatatt
    14401 tacacttcgg tgttaaatgc taaaaacgca ggtattaaat caatcagttt agatttaatg
    14461 tatcatttac cgaaacagac gattgaagat tttgaacaaa gtttagatct agctttagat
    14521 atggatattc aacatatttc gagttacggc ttaatacttg aacctaaaac ccaattttat
    14581 aatatgtata gaaaagggtt gcttaaactt cctaatgagg atttaggtgc tgacatgtat
    14641 cagttgctga tgtctaagat agaacaatct cctttccatc aatacgaaat atctaatttt
    14701 gcattagatg gccatgaatc agaacataat aaggtttact ggtttaatga ggaatattat
    14761 ggatttggag caggtgcaag tggttatgta gatggtgtgc gttatacgaa tatcaatcca
    14821 gtgaatcatt atatcaaagc tataaataaa gaaagtaaag caattttagt atcaaataaa
    14881 ccttctttga ctgagagaat ggaagaagaa atgtttcttg gtttgcgttt aaatgaaggt
    14941 gtgagtagta gtaggttcaa aaagaagttt gaccaatcta ttgaaagtgt ctttggtcaa
    15001 acaataaata atttaaaaga gaaggaatta attgtagaaa agaacgatgc gattgcacta
    15061 acaaagcgag ggaaagtcat aggtaatgag gtttttgaag ctttcctaat aaatgattaa
    15121 aaaaaattga aatttcgagt ctttaacatt gacttacttt gaccaatttg ataaattata
    15181 attagcactt gagataagtg agtgctaatg aggtgaaaac atgattacag ataggcaatt
    15241 gagtatatta aacgcaattg ttgaggatta tgttgatttt ggacaacccg ttggttctaa
    15301 aacactaatt gagcgacata acttgaatgt tagtcctgct acaattagaa atgagatgaa
    15361 acagcttgaa gatttaaact atatcgaaaa gacacatagt tcttcagggc gttcgccatc
    15421 acaattaggt tttaggtatt atgtcaatcg tttacttgaa caaacatctc atcaaaaaac
    15481 aaataaatta agacgattaa atcaattgtt agttgagaat caatatgatg tatcatcagc
    15541 attgacatat tttgcagatg aattatcaaa tatatctcaa tatacaactt tagttgttca
    15601 tcctaatcat aaacaagata ttatcaataa tgtacacttg attcgtgcta atcctaattt
    15661 agttataatg gttatcgtat tttcatcagg tcatgttgag catgtacatc ttgcttcaga
    15721 cataccgttt aataatgaca aactaaatac aatatcaaat tttgttacaa acaaactaac
    15781 tgagtttaat cagaatttac aagatgatat tgtctctttt gttcaatcag agcaggaaga
    15841 aatatttatc aataaattgt taaatacgat gaataatcat atttctaatc aaagtaatag
    15901 catttatatg ggtggaaagg ttaaacttat tgatgcattg aatgaaagta atgtatcttc
    15961 aattcagccc attctacaat atatagaatc aaatagaatt gcagaattat tacaagatat
    16021 atcttcacca aatattaatg taaaaattgg
//
LOCUS       DAMZLE010000054        15914 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMZLE010000054 DAMZLE010000000
VERSION     DAMZLE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.054"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..743
                     /gene="ccsA"
                     /locus_tag="POB92_001470"
     CDS             <1..743
                     /gene="ccsA"
                     /locus_tag="POB92_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDI6578977.1"
                     /translation="YDFVQKSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIPLGSI
                     SDVFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQKIAV
                     VDELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATLESIV
                     FYSTLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRINKWL
                     NSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            785..1711
                     /gene="hemC"
                     /locus_tag="POB92_001471"
     CDS             785..1711
                     /gene="hemC"
                     /locus_tag="POB92_001471"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDI6578978.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            1733..2401
                     /locus_tag="POB92_001472"
     CDS             1733..2401
                     /locus_tag="POB92_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDI6578979.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            2404..3378
                     /gene="hemB"
                     /locus_tag="POB92_001473"
     CDS             2404..3378
                     /gene="hemB"
                     /locus_tag="POB92_001473"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDI6578980.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            3426..4712
                     /gene="hemL"
                     /locus_tag="POB92_001474"
     CDS             3426..4712
                     /gene="hemL"
                     /locus_tag="POB92_001474"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6578981.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRNITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            4773..4874
                     /locus_tag="POB92_001475"
     CDS             4773..4874
                     /locus_tag="POB92_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578982.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            4908..5981
                     /locus_tag="POB92_001476"
     CDS             4908..5981
                     /locus_tag="POB92_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI6578983.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(6204..6764)
                     /locus_tag="POB92_001477"
     CDS             complement(6204..6764)
                     /locus_tag="POB92_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI6578984.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     misc_binding    6822..7123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7167..9797
                     /locus_tag="POB92_001478"
     CDS             7167..9797
                     /locus_tag="POB92_001478"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI6578985.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            9810..11081
                     /locus_tag="POB92_001479"
     CDS             9810..11081
                     /locus_tag="POB92_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI6578986.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERIGLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            11351..12058
                     /locus_tag="POB92_001480"
     CDS             11351..12058
                     /locus_tag="POB92_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6578987.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            12055..12741
                     /gene="radC"
                     /locus_tag="POB92_001481"
     CDS             12055..12741
                     /gene="radC"
                     /locus_tag="POB92_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI6578988.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            12809..13093
                     /locus_tag="POB92_001482"
     CDS             12809..13093
                     /locus_tag="POB92_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578989.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(13210..13407)
                     /locus_tag="POB92_001483"
     CDS             complement(13210..13407)
                     /locus_tag="POB92_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578990.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            13567..14040
                     /locus_tag="POB92_001484"
     CDS             13567..14040
                     /locus_tag="POB92_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDI6578991.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            14114..14233
                     /locus_tag="POB92_001485"
     CDS             14114..14233
                     /locus_tag="POB92_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6578992.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            14432..15274
                     /gene="mreC"
                     /locus_tag="POB92_001486"
     CDS             14432..15274
                     /gene="mreC"
                     /locus_tag="POB92_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI6578993.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            15274..15804
                     /gene="mreD"
                     /locus_tag="POB92_001487"
     CDS             15274..15804
                     /gene="mreD"
                     /locus_tag="POB92_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI6578994.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
BASE COUNT         5514 a         2161 c         2976 g         5263 t
ORIGIN      
        1 tttatgattt tgtccaaaaa agtcataaga ttagaagttt aggcatatat ttattgggga
       61 ttgtttgggt tttacaaaca atctctttat ctatttttat tatacaaact agacatattc
      121 cattagggtc tatttcagat gtattctata ctttaagttg gctgattatt tcaatctctt
      181 taattcttaa ccttatcaaa gttttgaatt tctctgtgtt tttcttgaat ttaatagggc
      241 taacactttt agggatgaat acatttcaac cgacacatta tactaataag gttcagaaaa
      301 ttgctgttgt agacgagtta ctgttagtcc atatcggtct agctgtgtta agttatgtct
      361 tctttgcctt agcttttgtc aatgcattac tgtatatcat gcaataccgt aatttgaagg
      421 aaaaacgttt tgatcaaaaa tactttagaa taggtagcgt tgctacactt gaatcaattg
      481 tgttttactc aacgttaagt gggtggataa ttctcatatt tagtattatt ttaggtacac
      541 agtggggtgt aatttcagtt ggtgaacgta tattcattga cccgaaggta atactctctt
      601 caattattac agtattatat ggaagttata ttttgcttag aatcaacaaa tggttgaatt
      661 caaggtattt aatatactat aacattattt tattttgctt aaatatgatt aatttatttt
      721 ttgcaacaca ttttgtgaat tgagaaaata atctagagca atccgttcct aggaggaatt
      781 gaacatgcgt aaattagtcg ttggctccag aagaagtaaa ttagctttaa cacaaagcca
      841 gcaatttatt gataaattaa aagctgtcga gccaaatcta gaaattgaaa ttaaagaaat
      901 tgtcacgaaa ggcgatcgta tagtagataa gcaattgtct aaagtcggag gcaaaggctt
      961 atttgttaaa gaaatacaac atgaactttt tgaaaaaaat atcgatatgg caatacactc
     1021 gcttaaagat gtaccaagtg taattccgga aggtttaaca ttaggttgta tccctgatag
     1081 agaattacct tttgatgcgt atatttctaa aacacataca ccactatccc aattgccaga
     1141 aggcagtatt attggtacta gttcattacg tcgtggtgca caaatattat ctaagtatcc
     1201 taatttagag attaaatgga ttagaggtaa tatagataca cgattagaaa agttacaaac
     1261 tgaagattat gatgcgatta ttttagctgc agctggttta agaagaatgg gctggtcaga
     1321 tgatattgta acatcttatc ttgatagaga tacattgtta cctgcaatcg gacaaggtgc
     1381 tttagggata gaatgtcgta gtgacgatga agaactatta acattattaa gcaaagtaca
     1441 taatgatgag gttgcaaaat gtgtgactgc tgaacgaacg tttttagcag aaatggatgg
     1501 tagttgtcag gtgccaatcg caggatatgc tacaatctca gatcaaaaag aaatcgaatt
     1561 tacaggttta attatgaccc cagatggtaa agaacgattt gaatatacaa tgaacggaac
     1621 agatccggtt gagttaggca aaacagtgag taacaaatta aaagagcaag gtgcttatga
     1681 aattataaaa cgcttaaatg aacaacatta aataatggag tgacgtttaa atatgaagcc
     1741 agttgtagtt atgacacaaa caaatgacat gcaaagtgat ttagtatcaa ttattcacaa
     1801 accctttatt gatataaagc cactaaattt tgatattcat ttgttaaatc aacgctatga
     1861 ctggcttatt ttttcatcta aaaatgctgt gaaattcttt tataaatatc taaaaggaat
     1921 taatgttgat aacattgctg tgataggaag taagacagcg caatattgtg aatcacttgg
     1981 cattcgagtt gattttatgc caaacgactt ttctcaagaa ggatttttaa aatcatttaa
     2041 tcaaactaac caaaaaatac ttttgccttc gagtgaattg gcgagaccat tgttattagc
     2101 agcgttatct aaagataatg aagttgttaa aatagattta tatacttcag tgcctaacaa
     2161 acaaaatata caagatgtta aagaaatgat agaacatcaa caaatcgatg cattaacatt
     2221 ttcaagttcg tcggcagtac gttattattt taatgaagga tttgtaccaa aattcaagtc
     2281 gtattttgct attggagaac aaacagcacg gaccattaaa tcatatcaac aaccagtaac
     2341 aattgcagaa attcaaacac tcgaatcact aattgaaaag attttagaaa gtaggggcta
     2401 aaaatgaaat ttgatagaca tagaagattg agatcatcag cgacaatgag agatatggtt
     2461 agagagaatc atgtaagaaa agaagattta atatatccaa tttttgtagt tgaaaaagac
     2521 gatgtgaaaa aagaaattaa gtcattgcca ggtgtatacc aaatcagttt gaatttactt
     2581 gaaagtgaat taaaagaagc ttatgactta ggcatacgtg ccattatgtt tttcggtgtt
     2641 ccaaactcaa aagatgatat aggtactggt gcatacattc acgatggtgt tattcaacag
     2701 gcaacacgta ttgctaaaaa aatgtatgat gacttattaa ttgttgcaga cacttgttta
     2761 tgtgaatata ctgatcatgg tcattgtggc gtgattgatg accatacaca tgacgttgac
     2821 aatgataaat cattgccact gcttgttaaa acagcaattt ctcaagtgga agctggtgct
     2881 gatattattg cgccaagtaa tatgatggat ggttttgttg ctgaaattcg tcgtggatta
     2941 gatgaagccg gctattacaa tattcctata atgagttatg gtgtcaagta tgcatcaagt
     3001 ttctttggac cttttagaga tgcagcagat tcagcgccat catttgggga tagaaaaacg
     3061 tatcagatgg accctgctaa ccgtttggaa gcacttcgtg aattagaaag tgatcttaaa
     3121 gaagggtgcg acatgatgat tgttaaacct gctctaagtt atttagacat agttcgagat
     3181 gttaaaaatc atacgaatgt tccagttgtt gcatataatg tgagtggaga atatagtatg
     3241 actaaagcag cggcacaaaa tggttggata gatgaagaac gtgtcgttat ggaacaaatg
     3301 gtttcaatga aacgtgcagg tgctgatatg attattacgt attttgcaaa ggacatttgt
     3361 cgctatttag ataaataagg ttttatatgt atgattttcc ataaactata ggaggaattt
     3421 actttatgag atatacgaaa tcagaagaag caatgaaggt tgctgaaact ttaatgcctg
     3481 gtggtgtaaa tagtccagta cgcgcattta aatcagtaga tacaccagca atttttatgg
     3541 atcacggtaa aggttcaaaa atttatgata tcgatggtaa cgagtatatc gactatgtac
     3601 taagttgggg gccacttatt ttaggacata gagaccctca agttattagt catttacatg
     3661 aagcaattga taaaggtaca agttttggtg catcaacatt acttgaaaat aaattggcgc
     3721 agctcgttat tgaccgagta ccttcaatag aaaaagtgcg tatggtgtca tctggtacag
     3781 aagctacatt ggatacttta agattagcac gtggttatac tggaagaaat aaaattgtga
     3841 aatttgaagg ttgctatcat ggtcatagtg attcgttatt aatcaaagct ggttctgggg
     3901 tggcaacatt aggattgccg gattctcctg gtgtgcctga aggtattgct aaaaatacaa
     3961 ttacagttcc atacaatgat ttagatgcac ttaaaatcgc tttcgaaaaa tttggagacg
     4021 atattgctgg tgtaatcgta gaacctgttg ctggtaatat gggtgtcgta ccgccgattg
     4081 aaggtttttt acagggatta agaaatatta cgactgaata cggcgcattg ctaattttcg
     4141 atgaagtaat gactggtttc agagtcggtt atcattgtgc acaaggttac tttggtgtga
     4201 caccagattt aacttgctta ggaaaagtta tcggtggagg actacctgta ggtgcttttg
     4261 gtggtaaaaa agaaatcatg gatcatatag caccattagg aaatatttat caagcgggta
     4321 cgttatcagg aaatcctctt gcaatgacaa gtggttatga aacgttaagc caattaacgc
     4381 cagagacata tgagtatttt aatatgttag gcgatatact tgaagacggt ttaaagcgtg
     4441 tatttgctaa acacaatgta ccaataactg taaatagagc aggttcaatg attggttatt
     4501 tcttaaatga aggacctgta actaattttg aacaagcgaa taaaagtgat ttgaaattat
     4561 ttgcagaaat gtatcgagaa atggcaaaag aaggtgtgtt tttaccacca tctcaatttg
     4621 aaggtacatt cctatctacg gcacacacga aagaagatat tgaaaaaacg attcaagcat
     4681 ttgatacggc tttaagtcgt attgtaaaat aaatatacgg acaaattgag agcctgaact
     4741 ttgttcaggc tctttttaaa tgtatatcag gcatgggcgg cgacttgata gtgaaagtcc
     4801 atatatgctt tgtagtcaaa actgctagcg gatattgtta tcttaacaaa cgtgaagctc
     4861 aagcagcaat ttagtcattt tattttttat tgaaagaagt ggaaaacatg acaatgatat
     4921 atagaaataa tttcattgtg ttcgttttat cattttttat tagtattcta ctgtattcat
     4981 cgcacgtatt actcccattt atgtttggtc ctattatcgc atcaatcatt tgtgtaaaag
     5041 ttttcaaact tgatattaaa tggccattct tacttagtga attaggtatt gtactattag
     5101 gtgtgcaaat cggatcaacg tttacgaaaa atgtcgttat ggatattaaa gacaattggc
     5161 tttcgattat tgttgtatct atttcgatat tattaattgc aatagtaatg gcatttattt
     5221 ttaaaaaaat tgcacgtatt aatacagaaa cagctatttt aagtgttata ccaggagcac
     5281 taacacaaat gctggtcatg gctgaacaag acaaacgtgc taatttgtta gttgttagct
     5341 taacgcaaac atcacgaatt atatttgttg ttgttttagt accgttcatt tcatattttt
     5401 ttcatgatgg taacatgcat gcgaatggaa agttaacaaa agtcttgcct ttatcacaag
     5461 tattaaacat agggcaaata gttattttag cgatagctat ctttatagtt tatctaatta
     5521 tgtctaaaat aaagtttcca acatttcaat tattagcacc actcattgta ttaattgttt
     5581 ggaatttttc tacaggttta acatttacac tagatcattg gttgttgaac atggcacaac
     5641 taatatatat gattagaatt ggagttcaaa ttgcgcattt attgtcagat ttaaaaggta
     5701 gactagcaat cgcaattaca attcaaaata ttatgttgat aattggtgcg ctaatcatgg
     5761 tttatgtcat acatttcttt gataataatc ctattaatga attgtttctt ggtgcagcac
     5821 caggaggggt taatcagata gttttagttg cattagctac tggtgccgat gttgctatga
     5881 ttacgagtta tcatattttc cgaatctttt tcatcctatt tattattgca ccaggaatta
     5941 attacttttt aaaatatcgt tcgaataagc gtgaagatta gaaactatca tccatttaag
     6001 ttgatttatg cttaggatag aattcattat taaggtttaa gcaaaggttg tacatctatt
     6061 tttagattaa gatatctaat attagcagtg cattaacttg gaactttaac ttatgatttg
     6121 aataaatttt cacatcaaaa aacgagacag tctattagtt aaaaatagat gatgtctcgt
     6181 ttactttatg atgctatagc aaattaattg tgtttaggct ttgatggaca atcttttaaa
     6241 tgtgcatcat ataaaccagc tgcttctaaa aacgaaaata ctgttactgg acctaaaaat
     6301 ttaaacccgt attgttttaa atctttagat agttgtgttg cagtatcatc aactgtgata
     6361 cgatcagaag catgttcata ctgcaaatct ttaggcttac catttacata agaccataaa
     6421 aatttactaa aactaccata tgcttgttca atttttaaat acccttgagc ttgattaaca
     6481 attgcttcta attttttacg atgatgaacg atatttggaa aagtcattaa gcggtcgata
     6541 tcttgagcgg tcatttgtgc taccttttct ggttcgaaat cataaaatgc ttcttcatag
     6601 gcttctttct tttttaaaat agttaaccaa gatagcccag catgttgtga ttctaatgct
     6661 aaaagtttaa acaatgcctt gctatcatag agcggttgtc cccatacatg atcatgatag
     6721 tttaagtaga ctggatcttt agtaccaaat gcgcattcat tcattttatt cccacctata
     6781 cattgactta ttttgtcaat cattatacta taagttaaga gtttaatata ggatttggga
     6841 agagtaagtt caaattattt taaagagagc atatgatttg gtgtgagtat gtaaatgatt
     6901 tttactgaag gtagcccaca gagtatctta gctgaacgtg attaatagta ggctaagacg
     6961 tgtttgagcg ttaatcataa tctagagtgc ttgaagagat gaagcatttc aaatttcacg
     7021 atgatagtat tggtatgatt attccaatta tgttaaaaga gtgaatttga tattaaacat
     7081 ccttcttgaa tataggtggt accacggaat atccgtccta tttgtatata ggatggatat
     7141 ttttattttt ttaggaggta tagcaaatgg aaatgaaacc aaaatatgat cctcgtgaag
     7201 ttgaagcggg acgttatgaa gaatgggtaa agaatggtta ttttaaaccg tcagaagata
     7261 aatcaaaaga aacatataca attgttatcc cgccaccaaa tgtaactggt aaattacatt
     7321 taggacatgc atgggatacg actttacaag atatcattac acgtatgaaa cgtatgcaag
     7381 gatacgatac gttatactta ccaggtatgg atcatgctgg tattgcgaca caggcaaagg
     7441 tagaagctaa attaaatgaa caaggaataa ctagatatga tcttggtcgt gaaaagtttt
     7501 tagaacaggc atgggattgg aaagaagagt atgcgtcatt tattcgtgcg caatgggcta
     7561 aattaggtct aggtttagat tatagtagag aacgttttac tttagatgaa ggtttaagta
     7621 aagcagttaa aaaagttttt gttgatttat acaataaagg aattatttat cgtggcgaac
     7681 gtattataaa ttgggatcct aaagcacgta cagctttatc tgatattgaa gtaatacatg
     7741 aagatgttca aggtgcgttt tatcatttta aatatcctta cgctgatggt gaaggtttta
     7801 ttgaaattgc aacaacaaga ccagaaacga tgttaggtga tacagcgatt gttgttaacc
     7861 ctaatgacga acgatacaaa gatgtaatcg gtaaaactgt tatattacca atcgtaggac
     7921 gcgaactgcc tattttagca gatgagtatg ttgatataga cttcggttct ggtgctatga
     7981 aagtgacacc agcacatgac cctaatgatt ttgaaattgg tcaaagacat caattagaaa
     8041 atattatcgt tatggatgaa aatggtaaaa tgaacgacaa agcgggtaaa tatgaaggta
     8101 tggaccgttt tgattgtcgt aaacagctag ttaaagattt aaaagaacaa gatttagtta
     8161 tcaagattga agatcatgtt cattctgtag gtcattcaga acgatctggc gctgttgttg
     8221 aaccatattt atcaacacaa tggtttgtgc gcatggaaga cttagcgaaa cgttcattag
     8281 ataaccaaaa aacagatgat cgtattgatt tttatccgca acgtttcgaa catacattta
     8341 accaatggat ggaaaatatt agagattgga cgatttcaag acaattatgg tggggtcatc
     8401 aaattccggc ttggtatcat aaagaaacag gcgaaatata tgttggagaa gaagcgccaa
     8461 ctgatattga aaattggcaa caagatgaag atgtattaga tacgtggttc tcaagtgctt
     8521 tatggccttt ctctacgtta ggttggcctg atttagaaag tgaagacttt aaacgatact
     8581 acccaacaaa tgccttagtt acaggttacg atattatctt tttctgggta gcacgcatga
     8641 tattccaagg cttagaattt acagatcgtc gtccatttaa tgatgtatta ttacacggtt
     8701 tagttcgtgc tgaagacggg cgtaagatga gtaaatcatt aggtaatggt gtggatccaa
     8761 tggatgttat tgacgaatac ggtgctgata gcttgcgtta cttcttagca acaggttcat
     8821 ctccaggaca tgatttaaga tactcaactg aaaaagttga gtcagtgtgg aactttatca
     8881 ataaaatctg gaatggggca cgtttcagtt taatgaatat cggtgaagac tttaaagttg
     8941 aagatatcga tttaagtggt aacttatcat tagcagataa atggattcta acacgtttaa
     9001 atgaaacgat tgcaacagtt actgatttaa gtgacaaata tgaattcggc gaagttggac
     9061 gtgcattata taatttcatt tgggatgatt tctgtgattg gtacattgaa atgagtaaaa
     9121 ttccaatgaa tagtaatgat gaagaacaaa aacaagttac acgttcagta ttgagttata
     9181 ctttagacaa tattatgaga atgctacatc cattcatgcc atttgtaaca gagaaaatat
     9241 ggcaaagttt accacatgaa ggtgacacaa ttgttaaagc ttcatggcca gaagtgcgtg
     9301 aatcattgat ttttgaagaa agtaaacaaa caatgcaaca acttgttgaa atcattaaat
     9361 ctgtaagaca atcacgtgta gaagtaaata cgccattgtc taaagaaata cctattttaa
     9421 ttcaagctaa agataaagaa attgaaacaa ctttatcaca aaacaaagat tatttaatca
     9481 aattctgtaa tcctagtacc ttaaatatta gcactgacgt ggaaattcct gagaaagcaa
     9541 tgacatcagt tgtaattgca ggtaaagtgg tattaccatt agaagggcta attgatatgg
     9601 ataaggaaat cagccgtttg gaaaaagaat tagctaaact tcaaagcgaa ttagatagag
     9661 tagataaaaa gctctctaat gaaaactttg taagtaaagc acctgaaaag gttataaatg
     9721 aagaaaaacg taaaaaacaa gattatcaag aaaaatatga tggtgtgaag gcaagaattg
     9781 aacaattaaa agcataggag ttagtaacaa tgaattacct agagagcttg tattggatac
     9841 acgaaagaac taaatttggc atcaaaccag gtgttaaacg tatggaatgg atgctagcac
     9901 aatttaataa tcctcaaaat aacattaagg gtattcatgt aggtggcaca aatggtaaag
     9961 gctctacagt tgcttacctt agaacagctt tagttgaaaa tggttatgaa gtaggtacat
    10021 ttacgtcgcc gtttattgaa acatttaatg aacgaattgg tctaaatggt gtgccaatat
    10081 caaatgacgc tattgtagaa ttagtatcac gtattaaacc agtaagtgaa atgatggaac
    10141 gtgaaacaga tttaggtgtt gcaactgaat tcgaaataat cacagcgatg atgtttttat
    10201 attttggtga aatacatcct gttgattttg tcattgttga ggctggattg ggtataaaga
    10261 acgattcgac aaatgtcttt acaccggttt tatcaatctt aactagtatc ggtctagacc
    10321 atacagatat tttaggtggt acttatctag atattgctag ggataaaggc gcgattataa
    10381 agcctaacgt tccagtgata tatgctgtta aaaatgaaga tgcattaaaa tatgttcgtg
    10441 aacgcgcaat tgaacaacat gcaaagccaa ttgaattaga tagagaaatt gttgttgtat
    10501 cgcaaaatga tgaatttact taccgttata aagattatga attagaaaca atcattttaa
    10561 gcatgttagg tgaacatcag aaacaaaatg ctgcattagc cataacagct cttattgaat
    10621 taaatgaaca aggattaatt gaattagatt tcaataagat gatagacggt attgaatcag
    10681 ttcgttggac tggacgtatt gagcaggtgc atgacaaacc tttaatcatt ttggatggcg
    10741 cacataattc agagagtata gatgctctaa ttgatacaat taaacagtac catgataaag
    10801 aaaaagtaga tattttgttc tcagcaataa acggaaaacc gattaacgag atggtcaaac
    10861 atttaagttt aattgcgcat acgttttatg caactgaatt tgattttccg aaagcgttac
    10921 gcaaagaaga aattgtaggt agtattgaaa atgatgaaat acaattagta gatgactacg
    10981 ttgaatttat aaaaaattat caaggtgata cattagtaat taccggtagt ctgtatttca
    11041 taagtgaagt taaatcaaaa ttagactttg aagcattata aaactaaaac taaaatagcc
    11101 atgaagaatc tatttaatca ataggtagag cttcatggtt attttttatg ctctaaatcg
    11161 tatgtgttta taaggttcaa tttcttgcca ttattaattt tgccgagcag taatgggttg
    11221 ttaaagtatc catcaattat gcttagcaat attgaaaaat ttaaatgaaa aagcggttca
    11281 attcttgaaa aatagtgtat atttacattt tggtaataac taactataat ttacataagg
    11341 aggttgtcat ttggtagtat tgttatctta tagttgcagt tgtattttta gttttctata
    11401 tcaatttata tctatagaag aaacgtcatt tgattattta catagaagat cgaaatgtga
    11461 ttattgtaat tcgtcactca aatggtatga attaatgccg attattagtt ttttattatt
    11521 aaaagggcga tgtcgaaact gtcgaaagcg tatttcccta acacatttct taggggaaac
    11581 ctttgcttta atacctatcg tctttattaa gtatgatttc acatacgtaa atgctacgct
    11641 atttataact acatatgttt ttctgcttat ttttactatg accgatatca cttctttaat
    11701 gttagattgt cgcttaatta taatttattg tatcgtttct ctctcgttaa gtatgattta
    11761 tccagtagct tttatcatta ttagtatgac cacgcatata ttctactttt tatttcgggc
    11821 atatattggt tatggtgacg ttttactaat atctgcactt tctttgtttt tccctctcca
    11881 attcactatt tatgtcattt tatttacatt tgtcattgct ggtttagttg ctttaattac
    11941 catgatattt aagccgatta aactattacc ccttgttcca tttatattta tttccttttt
    12001 tatcaattca cttttttata atgatatcca tcaattttta ggaggcgtat atttttgaaa
    12061 attaaagaaa tggtaacttc agaaatgcca agagaacgtt tgttaagcca tggtgcaaaa
    12121 agtctttcga atacagaatt attagctata ttaattaaca ccggaagaaa aggattctcg
    12181 agcatagaca ttagtaatga actgcttaaa tctgcttcga atctgaatga attgaaaaaa
    12241 tcttcaatta acgatttgat aaaagttaaa gggattggat tacaaaaagc aattacttta
    12301 aaagcagcat ttgagttagg ggaaaggatg gggagaagag ctgaaaataa tcgtataaaa
    12361 ataacgcaac caagtgatgt tgctgattat atgattccaa caatgaaaga tttaacacaa
    12421 gaacattttg tcattttatt gttaaattca aaaaatgtag tgattaaaga aacctgtgtt
    12481 tttaaaggta cattaaatag ttcgattgta catccacgtg aaatttttag tattgcggtg
    12541 agagaaaatg ccaatgcaat catcgcagtt cataatcatc catccggtga tgtaacgccc
    12601 tcacaagaag atatcataac aacaatgagg ttgaaggagt gtggtttgat tttagggata
    12661 gatttattgg atcatattat aatcggtgat aatagattta ccagtcttgt agaagcgggt
    12721 tactttgatg aaaatgattg aagttgatag aattaatgac gtatcttgtg tataatacct
    12781 acgaagtact ttcattggag gaaaaatagt gactctattt attattatcg gggttctcgt
    12841 gccaatggtt tataccatgc agttaaatat taaaaatgaa cctgtaacaa agcgcaatct
    12901 attaataaca ttagctttat ctacgttagg tattttagta acggcgttag caggtgtgat
    12961 cgttacgaaa caagcttttc ctttattaag tgtagcaatc ggctcaattt ttactggaat
    13021 cgtttggggc cttttactaa gtggtagcta cgcgctgata cgatttttat ctaacgcatt
    13081 tgggcgtaag taatagtaat ctgttcatca agtagtatcc gtgcttgaaa ccaaattaaa
    13141 actcctaatg tggaactagt taaaaaattc taaactagta cattaggagt tttgttatgc
    13201 agaataaatt taattgttaa attgaaagtg cggtaaaaac ccactattta tttgaaaaaa
    13261 atcgagcaac caaattaaac cttgatatcc taagtaaata cataataaaa caagtccgac
    13321 ataaattaaa aatcgcaaaa tagaaagtcc aactctaaaa aggaggatga ctaatagtgc
    13381 tattaaaata attgttagta tactcaatgc tacaaacctc ctcatacgct tttaaatcat
    13441 aacaaaatta taagctattt taaatgaacg tgcatcagtc ctaagatata tcatgattaa
    13501 ggctgaagac tgaggaaaat atacataata aaatatataa ttagatgaag acattggagg
    13561 cgaactatga gattgatatt tagtatgatt aaaaacatta tagcagtaat agcaattgtg
    13621 ataattgttt atatcgcctt gaaatacgca ccttttttaa aagaacaaga ttggaatcct
    13681 gtaaatcacg ataatgatca aatgaatcaa gttacgcaac ccactaatga tgcccaacat
    13741 gtttatgtaa gtggtgagaa atattcatta aaagaaaatg atttaataaa gaatgtacca
    13801 ttaagccaaa ttaaaaatgt atttaaaatg atagataaac aagagtttat ggctgtgtct
    13861 ggaatgaatc gcatggctta taatgatcaa tatattatag gtcaaagagg agacgaattt
    13921 attctttata aatttggaga tgagtcaatg cgtgtttaca atactgaatt tgaaatgcaa
    13981 caagacttaa atgaattagg gcaaaattta caactaaaac ccgaaaatgc ttatcaatag
    14041 gaaattattg cataagaaat atattttagt tatcctgtca ctagaataaa tttaaaaggt
    14101 tggtgtagtt caaatggcag aacaatcaaa acagaaacaa gctaatgaac aacaaaaggc
    14161 ccagaactta ttcgcacgtt ggagacaact tcagaattcg aacagtgaat cctcgaacga
    14221 tacaaacaaa taattttaaa atttaaatcg attatttaaa ctactagtga ctggaatgtt
    14281 tgctagtagt tttttgtttt taaaatgcca caaatttttt acggagttgt tcattttcaa
    14341 agtaattcgt agttagtgct ttatttctta atgggagtaa tataaaatag aaaaggatat
    14401 acaatatttt gacataatag aggtgttctg ggtgcttaag ttttttaaaa ataacaaatt
    14461 aattgttgtt ttatgtgcaa ttatcgtttt tattgcatta attgggctgt ccatacgttc
    14521 acaatctcaa tcacctcctg aacaatatat aggtgattct gtgtcttttg gacaacgagt
    14581 tgtgagttat ccagttaatt ttgttgctgg tacgattggg gactttttta aaaaaggaga
    14641 ttctaaagaa tctaaaaata agattagcca gttagaatct aagaaccaac aattagaagc
    14701 ggaaaatgaa aaattaaaaa aagagcttga tttaaaagat atttcaaaat ttgatcctat
    14761 ttctactacg gttttagcaa gaaatccgga tcagtggatg aatacaattg taattgataa
    14821 gggatctaaa tcaggtataa cttcaaatat ggctgtgatg acatcacaag gttttgttgg
    14881 aagagttact aaagttaata aattttcttc acaagttgat ttaatctcaa ctaatacacg
    14941 tgcgggtaaa ttatctgtaa atatacaaca cggttctaaa aatatatttg gtttaattga
    15001 tcgttatgat gaaaagaact cagaacttgt aattagtgac attaataata gagataatat
    15061 ctcaaaaggt gataaagtcg ttacaagtgg attagctgat caactaccaa gtaatttata
    15121 tataggagaa gtgactaagg ttcaaaatga tcaatacggc ttagctaaag aagttagggt
    15181 taagactggt gcagacttaa cagatttgag tcatgtttat gttgcaaaaa gagatcctaa
    15241 aacaattcct gatgatgaaa gcagggataa ataatgcgta cactgtatta ttttttgata
    15301 ggtattttac tattttatat agatactgca attgggcttc ttattccaat gcatataggt
    15361 aagtttgaac ttgtatttgt accacacctt acatttatgt atattttaat gatggtagtg
    15421 tatcgtggct ttggcgtatc attattgctc agcatatttt taggcgtaat gactgatgta
    15481 tactttggta gtatttatgg agtgtactta tttggctata tattgttttt agcacttata
    15541 gatcgattct ttaaaatttt ttacaaagat cattcgatgt tattcattat tatattagcc
    15601 agcaccttat tattagaagt ctatgtggca ttaatatacg gtatgttagg attcattcaa
    15661 tttgatatta ttcattttgt agtctttaga ttattgccaa cattaattat gaattttgta
    15721 ctgttgatta tgctttatcc gttgattata aagttcctta aaaaaacaaa caatgacatt
    15781 gacatgaaac gtcgtcaatg gtaagatgca atagttgagt agttataagt agctacatac
    15841 aaccgctcaa atataggttc aagaacattt ttaatgaaga aagcgattgt caatcaaggc
    15901 gttgaaggaa gaaa
//
LOCUS       DAMZLE010000055        15793 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMZLE010000055 DAMZLE010000000
VERSION     DAMZLE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.055"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(433..3480)
                     /gene="fnbA"
                     /locus_tag="POB92_001488"
     CDS             complement(433..3480)
                     /gene="fnbA"
                     /locus_tag="POB92_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDI6578995.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATENKVNETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEEAPKAVQAP
                     QTAQPANLETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVVEAKEASDEKVETGTDVTSKVTVESGSIEAPQGNKVEP
                     HAGQRVVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEI
                     LGNGNIRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPV
                     VYNPGVSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAG
                     GQPTVKVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLD
                     KTYVIHYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADG
                     NGKNGQIIQNNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGG
                     YVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIQGQNNGNQSFEEDTEK
                     DKPKYEQGGNIIDIDFDSVPQIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTL
                     PKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSE
                     PETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKA
                     QSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNNKA"
     gene            complement(3464..3566)
                     /locus_tag="POB92_001489"
                     /pseudo
     CDS             complement(3464..3566)
                     /locus_tag="POB92_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3949..5307)
                     /locus_tag="POB92_001490"
     CDS             complement(3949..5307)
                     /locus_tag="POB92_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDI6578996.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(5424..6977)
                     /gene="gntK"
                     /locus_tag="POB92_001491"
     CDS             complement(5424..6977)
                     /gene="gntK"
                     /locus_tag="POB92_001491"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI6578997.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQKHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHMPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(7001..7681)
                     /locus_tag="POB92_001492"
     CDS             complement(7001..7681)
                     /locus_tag="POB92_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6578998.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(7835..8599)
                     /locus_tag="POB92_001493"
     CDS             complement(7835..8599)
                     /locus_tag="POB92_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6578999.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNKASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWQVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(8764..9456)
                     /locus_tag="POB92_001494"
     CDS             complement(8764..9456)
                     /locus_tag="POB92_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI6579000.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(9687..10103)
                     /locus_tag="POB92_001495"
     CDS             complement(9687..10103)
                     /locus_tag="POB92_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDI6579001.1"
                     /translation="MPFTMKGYPEKWKDFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(10242..12065)
                     /locus_tag="POB92_001496"
     CDS             complement(10242..12065)
                     /locus_tag="POB92_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6579002.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDNFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHIMKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNSFCERDKNV"
     gene            complement(12974..14251)
                     /locus_tag="POB92_001497"
     CDS             complement(12974..14251)
                     /locus_tag="POB92_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579003.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            14798..15409
                     /locus_tag="POB92_001498"
     CDS             14798..15409
                     /locus_tag="POB92_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6579004.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
BASE COUNT         4762 a         3041 c         2310 g         5680 t
ORIGIN      
        1 catctaattc aaaacaatat actaaaacat acataataat cgcctgtaca atgcatcatt
       61 aacaagtcac tgaaacgcct ttcattgtat taataacgtc actacaattt ttatatcgtt
      121 cggtttttgt ttggttttaa tgattgttta tacagaaaca gccgtatttc aagacgatat
      181 tttaaattta actaaatttg catctagtta ataattgcat ttatctaatt tgtcttattg
      241 attcaatcta atttgtactt acaaactagt tttaaattct aactttatct ctcagttcgt
      301 tatcaatcat cagacataaa ccaatgaagc aatcagaaaa cactctaatt ttctattaga
      361 aatttgattt aatctaaaaa aacaggttta cttcatataa tttatgaaat aaacccgtca
      421 atttttgttt aattatgctt tgttattctt tttatttctg cgtaataatg ctaaacctag
      481 aatgctgaat aatccgccga acaacatacc tttgtttgtt gattcttctc cacctgtttc
      541 aggtagttca gatttcttag attgtgcttt tttagttggt gccactgctt taaccttttc
      601 attgatttca ataacaggtg ttactacttt accttgttcc actggtttag aaggcttttt
      661 aggttcttct tttgcgggtg gtactggttt accaggttca gctggtacct ctggtgttgg
      721 cggtgttggt gtttctggct cactcggcac ttctggtgtc ggtggtgttg gtgtttccgg
      781 ctcacttggt acttccggag tcggtggtgt tggtgtttcc ggctcacttg gtacttctgg
      841 tgtcggtggt gttggcggcg ttgtatcttc ttcaatcgtt tgttgacctt cattttgacc
      901 gcttactttt ggaagtgtat cttcttcaaa atcaacactg ttgtgtccac cgaattgata
      961 atttggttta tctttgtttg tatcttcttc aataatttca gtgtgcttat tgaatccatg
     1021 aatttgtggc acactgtcga agtcgatatc aatgatatta ccaccttgtt catacttagg
     1081 tttgtctttc tctgtatctt cttcgaatga ctggttacca ttattttgac cttggatttg
     1141 aggtacactg tcgaaatcga tatctacgat attgccacct tgttcatatt taggtttgtc
     1201 ttcttctgtg tcttcctcga atgactggtt accgctattt tggccacctt cataacctaa
     1261 ttcactctta atatcaacat gactattttc ttcgatttct tcaatcacgc cataattacc
     1321 gtgaccattt tcagttccta aaccagaatg agaaatatga tgattgtttt cagtgatttc
     1381 ctcgattgga ccttgagctt gaccatgttc ttctggtagt tcatccacta gttcaatcag
     1441 attactttca gtcgtatatt ctttcgtatc ttcaattgtt gtatgatcgc tcactgcgcc
     1501 agttacaata ccttttgtag actcttcgtc aaattcaact aagttagact cagtagtaac
     1561 ctgaccacca cctggatttg tatcctcttc atattcaaca acatcagcgt gatgttttga
     1621 attttcatgt gtcgattctt caaagtcaat tggatttgat tcctcagagg actcagtgta
     1681 tcctccaacg tgacccgctt cactatccac agcagtatgg taatcgatat caatagctga
     1741 tgaatccgtt tcttctattg tttcaatata cccatcaaca taaccaccct caccatctat
     1801 agctgtgtgg taatcaatgt caagcggtgt attgtcttga ttttcttctg tttcaataat
     1861 ttgcttggca tcgtactgcc cgctcatagt tccttttgca gtatcttctt tatattcaaa
     1921 atcattattt tgaataattt gtccattttt accattgccg tcagctttat tgctatataa
     1981 aactaaacca ttatcccaag ttaaacgata tcccccataa acatagtaag atttatatgc
     2041 tcgttctgga tacccatata attcagttct aaaattaacc tgatctgacc cttgcaaata
     2101 ttcacctgta taatgaatga catacgtttt atccaactta tctaaattca atgagtaact
     2161 accattgtct tgcacactca attttccatt catttccttt gttacatctt tgaatttgtt
     2221 agtatctgat gtatttgcat aaacactttg tggcaattca tcttttttcc ctagatattc
     2281 atatacttta acagtaggtt gtccaccagc taaattacta ccttcagtca acgtccctgt
     2341 tactgataca gtgtttgact ggtttccatt cattggctta atataagcta tatgtgtaaa
     2401 tttattagat tctttattaa atgtttcaat tgatccattt acatttgtat aactattgct
     2461 aacacctgga ttataaacaa ctggtattgt tttttctgtt tcttcaccat ttaatttaga
     2521 agtaatcttt tgttctccat tgctttgtac agttttaggg tcaataaata agttgatttc
     2581 taaattagct gttacctcta ccttgtgttc aatttcgtta gtaaatgtat atcttatgtt
     2641 accattccct aagatctcac ctgtagccat tacaactgag ccatttttaa tctctggtac
     2701 ctttctagct gttgaaaccc cataagtatt tacattattt gataatgtaa aatcaaaata
     2761 atctcctctt tttaatccat ctgcgaattt caatttgtat ttcaatacga cacgttgacc
     2821 agcatgtggc tctactttat ttccttgagg tgcctcaata gaaccacttt ctactgtaac
     2881 tttacttgtc acatctgtac ccgtttccac tttctcgtca ctagcttcct tagcttcaac
     2941 tacgtctgct gatcttgtca cacgtggttt actttctgat gccgttcttg gctgtgccac
     3001 ttcaacttgt gtttctgcga cttgattttg tgtggccttt ttaggtgtta aatctacttg
     3061 tctttgatct ccgctattgt cttgagattg tgttgtttcc ttaacttgag gtttcgcttc
     3121 ttccttaact acctcttctt taactgtttc taaatttgct ggttgtgcag tttgtggtgc
     3181 ttgtactgct tttggtgctt cttcagttgt tacttgtgtt gcgtttgacg gttgttctgt
     3241 tgctgttgcg ctgtatgatt gtgtttcatc tatagtatta acgttagttg tagttgtttg
     3301 tgtttcgttt actttatttt cagtagctga attcccattt tcttctactg tagttgtctt
     3361 ttgttctgat gctgcagctt ctttatcttg tcccatccca acaacgatca ttgttcctaa
     3421 gaatactgat gctgctccca atttatgttt tctaatgccg tacctaagat tgtttttcac
     3481 tataatatct ccctttaaat gcaaaattca ttaatttttt taaacttaat aaatgcaagt
     3541 ctatattgtt cggtttttaa aagcaataca aaaaagcgag ttaatgaaaa gttaagattg
     3601 tcattaattt tatgttaatt gataatttta ttatttgaaa tatacctata aattgtattc
     3661 aagtcatcat aaacccttgt cacacaaggc ttgtattttt tatacttatt tttcaaatta
     3721 aattcatcat tatctaattt aaaacaatat actaagtgtt tcataattat cgcctgtaca
     3781 atacgcacaa aaacatgtct tgaaacgcct ttcattacac taaaatcccc aatatacttt
     3841 ttatatcgtt cggattatga ctatttcaga cgattttctg cataaaaata aacgtgtttc
     3901 aaggcaatat attgcaatta ccttaaaaca cgtttactta atatttagtt aaacaaataa
     3961 gctgatgaat agaatgaaga tgatacctga aacggaaata atcgtttcta ataatgacca
     4021 tgttaagaat gtttctttta cagttaaacc aaaatattct ttaaacatcc aaaatcctgc
     4081 gtcatttaca tgagacaaaa tcacactacc tgcacctatc gcaagtacaa ctaatgcaac
     4141 atttacatct gatgattgta ataatggtaa gacaatacct gtagttgaaa tcgcagctac
     4201 tgtagccgaa cctaatgcga tacgtagcac agctgcaaca atccatgcta gtaaaattgg
     4261 agacatctct gtaccttcaa acattttagc aattgtattt ccgacaccac cgtcaattaa
     4321 tacttgttta aatgtaccgc caccgccaat aatcaataac atcattccga ttggataaat
     4381 cgcattcgtc actgattcca taatatgatt catcttacgt tttctcatta atcccatcgt
     4441 aacgattgca aataatactg ctattagcat ggctgtccct gctgttccta tcatataaat
     4501 gatagattca aatagatttg taggtttgtc gtgcccagtt acaagttgcg ttatcgtaga
     4561 cactaacatt aatatgactg gtaatgttgc agttaataaa ctcataccaa atcctggcat
     4621 ctcttgatcc gtaaattctt tttgtgcacc taacgctgaa atatcgcctt ctcgtgtata
     4681 cgcagacgga atcatttttt gtgcaacttt gttaaatata ggtcctgcaa tgagtgtaac
     4741 tggaatggca ataatcatac cgtacagtaa tacatctcca acatttgcct ttaattcttt
     4801 tgcgatgact accggtcctg gatgtggtgg taaaaagcca tgtgtcacag ataaagctgt
     4861 taccataggt agtcctagtt ttaacactga aacatttgcg cgttttgcta ctgtaaatac
     4921 taatggaatc agtaagacta aacctacttc aaagaacaat gcaataccga cgataaatgc
     4981 tgcaacaagc attgcccatt gtacatgttt ttgaccaaat ttttgaatca acgtgtctgc
     5041 aattcgagtt gcaccaccac catcagcaag taatttccca agtatggcac ctaaaccgaa
     5101 tattagtgca atgtggccga gcgtactgcc catccctttc tcaatcgtct ccataatttt
     5161 agtcaatggt atacctagca ttaacgctgt aatcatcgat gtgataatta atgaaataaa
     5221 tgtatttaat ttaaacccaa taattaatac taataaaata acgataccta aaacaacact
     5281 gattaacggc catatttcgt taaacatgac attcccctct ttctcttttc aatagaatgt
     5341 aacaccgtcg tcgcgcacgt attccacata atatatagta gtcatggcac aacaacttta
     5401 tctaagatgc ttaagtgacg tatttattgt gttttatttt cagccatatg ttggcgttga
     5461 aaatctgcaa tttgttcata attctctgtt aaagaacgac ttaaattgat aaaaatggat
     5521 acgagctctt ggtaaacagc gacattttct tcaaacggca tatgattatt tgtagcaccg
     5581 accatcgatg aaacgattga aaaatcttca atgtcaccta cagctttaag tccaagcacg
     5641 caggcaccta agcatgaact ttcatagctt tcaggaacca ctaactctgt gtcaaatata
     5701 tctgacatca tttgacgcca tacttcactt ttcgcaaaac cacctgttgc ttttatcatc
     5761 ttaggcgttt cattcattac ttcaatgagt gcaaggtaga cggtatacaa attgtaaaga
     5821 acaccttcta atgcagcgcg aatcatatgt tcttttttat gagataaagt taaaccgaag
     5881 aatgaacctc ttgcatttgc gttccaaagc ggcgcacgtt ctccagctaa atagggatgg
     5941 aatattaaac catctgcacc tggtttaaca cgctttgcaa tttgagttaa gacatcataa
     6001 ggatcaacac cgagacgttt cgcagtttcg acttcactcg ctagcaactc gtcgcgcaac
     6061 catctcaata cgacaccacc attatttaca ggacctccga tgacgtagtg gtcctctgtt
     6121 aagacataac aaaatattct acctttgtaa tcagtacgcg gtttatctat cacagtacga
     6181 atcgccccag atgtaccgat tgtgacagca acttctcctt taccaacgct attgacaccc
     6241 aaattagaaa ggaccccatc actcgcacca ataacaaacg gtgtatcttc attaagcccc
     6301 attaatgttg cataacgttc tttcatgcct ttcattacgt acgttgttgg cactaattcc
     6361 ggcaacattt ccttggaaat accaagcaat tctaatgccc caacatccca atctaatgtt
     6421 tctaaattaa acatccctgt tgcggaagcc attgaataat caatgatata tgtatcaaat
     6481 aaatgataga aaatgtatgt tttaatatct gcaaacttag cagtacgttg aaatacatct
     6541 tgccattcat gtttcatcca aaaaattttc actaaaggcg acataggatg aataggcgta
     6601 cctgttcgct gataaatcgc attgccatca tgcacttcat ttattactgt tgcatatttt
     6661 gcagcgcggt tatctgccca agtaatatta tttgttaatc tttgatgttt ctgatccatc
     6721 gcaatcaagc tatgcatttg cgcactaaat gacacaaatt taatatcatc tttattaact
     6781 ttggattctc tcataacata tttaatagtc attaataccg catcaaataa ttcatctggg
     6841 ttttcttctg agacatcaac gtttggtgtg tgtaaatcat agccgatttg atgtttcatg
     6901 atgaatgttc cattttcatc atataagact gacttggtac tcgtcgttcc aatgtcgaca
     6961 ccaatcatat atttcatgac aaatccttct ttctttcatt ttaattcaac caaaatcctt
     7021 caatatcttt accaacatcg tcgaaattta agtgaaacgc ttctttcaaa atttgactgt
     7081 cgtattgttc cactgcatca ataaacactt gatgattatg atgtatgcgt tcaaaatctt
     7141 gcgggttctg ttgcatacgt tgtcgcatgg atgttaaaac gagtgccatc attactggtt
     7201 ttaaatgact ccaaaatgag tttaaatatt gatgattaga tgctttgatt aatgtttcat
     7261 gaaattcaaa gtcatgcttc gtaaatgatt ctgcatcctc aaattttact gccactttca
     7321 tcatttcaag ttgtttcttc atttctttta cgataggtag tcgctcttga tttttaactc
     7381 ttgaaaatgc aaaagactct agcatcagtc gcaaatcgta catttctttc ttttcttgtt
     7441 ccccaaacgg caacacatgt gcacccattc tttctaattg gatgagttga ttttgttgca
     7501 ataatttaaa tgcatctcga attggcgaac gactcacatt aaattgcttt gccatttgat
     7561 tttcagtgag taacgtacct tcagctatgt gaccattcac aatacctaag cgtaattctg
     7621 ccgcgatacc ttctccagtt gtcatacctt ccaaccattt ctctggatat ccatacatca
     7681 tcaaagccac tccttcatta cacgacatac ttgtatacaa gtatgttaat atagttctta
     7741 atcgtttgca agcgctttct ttacgcgtac taaaatagtg accatccctt ttcaatgtaa
     7801 atttaaaggg aatggtcact atcacacgaa tgatttaatt gttatgttgt atgtgggata
     7861 tttctaattg ttctgtactc atatgcgctt taggtacttc aatgcaataa tgcgtttcat
     7921 gacagtttgg acattcgaat cgacgtgttg tagcagtatg tttcgctttg ataacttgcc
     7981 ataaagatgg tgagaatata tgctggcagt taggacataa ataggcaacc ttttgttggt
     8041 aataaaaagt aacaccaatg ccgtatccaa tcataaacgg caacgcaatt agaaacggcc
     8101 atttattttt catcaaaatt gcacttataa tgctagaata ttgaattatt cctataatac
     8161 ccgcactaat ccaaatgtta cgacgaatac ttttcatttc agctgattta ctcatgacat
     8221 gctctatgtc ttttaaatgt gtgattggag acgtcgacgc tttatttacg taatattgaa
     8281 catttttaat tttgtttaat accgcttgtt gctgtttgac ttgttgatta atttcttgtt
     8341 gtttcatagt tagtaaagta ttgagtgtct tcaaagtacc ttcacctttt agcaacatat
     8401 ctatatcgct taacgcacaa cctaaatctt taagcaataa gattaactct aatgtttgtc
     8461 gctgttgttc tgtatacaca cgacgctttc cttctgtaaa tccttgtggt ttcaaaatac
     8521 ctttgcgatc ataatattga atcgttcgcg ttgtcacatt gcataatttc gcgagttctc
     8581 cagtcgaata gttagacata gattccacct cctacaatca ccatagttga tgacccgacg
     8641 tcacgagcaa gtactttttc tacattttaa agaaatttat tatactaggc gtcttatttt
     8701 tgtgatttcg taccatgttg atttacaaaa tcactcaaat taagcaacac tactactaaa
     8761 cgtctactct gttatttcag aatgaatttg ttgtaattta tcttcaactt cagtaatctc
     8821 tgtcgcacat tctttcagta aatctcgata cttttccgtc tctgcattgt ttttataacg
     8881 tattttatgt tctaaacttg cccacatatc catacctatc gttctaattt gaatttcaac
     8941 aggcaatacc tcaacgcgtt ctgctaaaaa gactggaatg gatacaacga tatgtaaact
     9001 gcggtaacca ttttctttag ggtgctgaat ataatcttta cgttttatca attgtacgtc
     9061 ttcttgttta agcaacatct cttcaatcaa ataaatatca tctaagtaat tacatactac
     9121 gcgaattccg gcaatatcca gtatatgttc tttggcagtt tctgcgctaa tctgtaatcc
     9181 tttacgatta agcttttcta ttaaactgcg catttctttc acacgtcgct ccatatgatg
     9241 tatagggttg tgtttataaa tgtgattgaa attatcgtct aaaatactca gtttagtgct
     9301 tatttccttt agtgctgacg aataaatatg atctaactct acgaaaccta ataacgtatc
     9361 aaatgcttca tcaccatttt caaatttact taaactattt ttaaaatcat gtcgcaaatc
     9421 ctctaaatat agtgatggtt ttcgatctac atacattttt atactaacct ccgatatata
     9481 atcatcttta ttgtacctaa cattttatta agataatact aatacactat cgaactttgg
     9541 gctgatactg aaacagcaca aagaaactca aacaattcga attatgtatc aaaaccttca
     9601 attataggaa gtaattttaa aatgatttaa cgcaaaaaaa cacctgttac cgttatatag
     9661 gtacaagtgt ttaattgata gagatgttat acgtctttaa atgattccac aacttttgga
     9721 tgtggaccat ccataagcgg ttctctttgt cgaacgccac cactttggtt gccaattgat
     9781 tcgctatcaa agtacgattt atcttgattt gattgttctt gaatatgttc ttcattatca
     9841 gttgttgcat attcactatc cacctctgtt ttttccattg ttgctgtatg gaattgtacg
     9901 aagttctctt cttctaatgc tttgatttct tctttcgata acgctcgata ccaatctttc
     9961 gcttttttcg ccgcaatagg aacaacatct ttttcaagat atccttcttt taacattgca
    10021 ttcgcaattt ctatcgcttt ttgccgttta attacttcaa aatctttcca tttttcgggg
    10081 taacctttca ttgtaaaggg catttcctta acctccaata tgttataatt catgttatat
    10141 accaccataa ttataaaatg aaacatatta aacacaattt tttagactcc cgtcataata
    10201 acttcagaca aacgttagtc tgacctgata aatattttat tttaaacgtt cttgtctctc
    10261 tcacaaaaac tattgtgttg aatattcatc attgctgatt gaatataatt atactgtggt
    10321 aaatctggta atatctttat ttctaattct gtatttaatt ctaaatgctt agcgatgttt
    10381 tcaaatgttg ctaaatactc gcccattaat tgttcattta ttgtaagtct atcttcatta
    10441 gccatggctc tatttaacat aaagctaatt tcttctaatg cgaataagct aggataataa
    10501 ttttgaatca atgtcttatc actaaataat tcaccattag ctgcattata aacttgcgtc
    10561 atgttattta atttcacaga taggttcata gccgtgtttt tctgatacac atcatcttta
    10621 tattggtttt cagaaaacaa ataatggaag agcgttgctt ctttacgaac aacttcagcc
    10681 attacattag gaagcaatgc ggatgcggta cgttgtccaa ggacaaataa acctatgatt
    10741 gcaatcacaa tacccattgc aacgtcaata actcttggaa acgcaatgtt aattgttaaa
    10801 ttctgtgatg ctaatccatt cattaaaata acttgtattg taataaaaac tactgcaatc
    10861 gcataatttg ctcccaccaa tgcttcagta aacaatgctg caatgcccat taaaattact
    10921 gcaacaggtg ttggtattga aaacaacaat atgactgaaa gtactaacac acctaaaata
    10981 gtacctaaac ctcgtgccat acctctctcg attgcatgta tagttgatgt tcctagtaat
    11041 attgtatgtg cagataacgg tatccagtat gctttttcaa aatcaaacat taacgcaata
    11101 aatatcgcta tcatcataat cgctgtatat ctcaatgtat ttctaaaaac aatagagtct
    11161 aatgttaaat tttgataaat gcgtttacta tataaaggtt ttcgcatgtc tgcttcatac
    11221 gcaatacgcg cttcatttgc atgcaccatt tcatcaattt tcattatatg gttgaataaa
    11281 ttttgaaatt cctctgttac tgtcacttct tttcgccaat atcttacatt ttcctcacta
    11341 ttatctagtt gtgcaatgat atgatccatc atttcaatta actcatctgg caatggtcga
    11401 atttgtttag cgttcaactc taacagttca gaataaatcc cttgggcaga tgtgtgtaat
    11461 aataataatt tttggaaacg tctacttaat ttatcgttac ttgaagtaga agtgattaat
    11521 aatttatcag atgctttaaa attatccacc gctgtttttg ctacttgtaa aaatgcagat
    11581 ttatcattat aagtgtgtaa caacttagat atgagtttaa aatcattttg aattgcttgt
    11641 tcttcagctt tgtttttaga aaatacgatc gtgattaaca ccatcatagt ggcaagtata
    11701 ccgcctacta aaatcgcaaa gcctctatat aatgcttctt cgggagctat aggtaagtta
    11761 atgggtagac tgaatgtcac aatgaagaat gtcgatgacg gtccagctat ttttaaggca
    11821 gtaaatatat aaaatggtat aaccgtaaca attaataatg tcattccaaa aacgagtggc
    11881 gttttggctg ttagcgtacc aagcatcata catattgcaa acgctaaatt acaaattatt
    11941 acagttcgca gcttagatcg cgacggacct ttaaaaacat aaatatgggc tagtgttccg
    12001 gttgcaacta ataatccaaa ttggaaatta ccacataagt aaccgattat tgcgggaata
    12061 atcattaaca gtccctgtct tacaccttta taaaagtcta agttaagtcg attgattttt
    12121 atgagtgatg tcaaaaagat gtcatttcga cttctccttt ccttgtcaaa atatttccat
    12181 aatcttttta ttagcatagt taaaatgaat ttgttgctat aaattttaat tacgcaacat
    12241 atgtcattat aacaccttat actcaattat ctaaattcaa aaacttttaa agtccaatta
    12301 aaattatttt ctaatgttat ttaacatcgt tgaacctttg gtcgcccacc tttgcgctag
    12361 ttaagattta tttgtgacat gttgaaatta ttttagtgaa tacgatattt agggttagaa
    12421 aaacattcac atagacttaa ggtttattta cttcatatta ttcctagggt tcatgatcca
    12481 aactatagtg aatttaaaaa agtttataaa acatatttgc ctcataagct tgaatagttc
    12541 ctatattcgt tatttaaaat gacattcgat gtcagttcat ttcttaaaat atcgaaagat
    12601 gattacgtat aatatatata gtgacgtatt tacaaataat ggcagtaagc agtacaaaat
    12661 gattacacaa aaatttcctt ctactcgata aattaagaag tataaaataa ttaaaaaaat
    12721 acttataatc agacctataa gaggcaaact tctaatatat ttctctaagt tttccttcat
    12781 atgtataacc tcctaaaatt tatatgtact tttacaagcg ttacctgact aatgttttaa
    12841 aactttattt ctttacatat atcattttaa taacttcccg ttcatcttaa accatatctg
    12901 ttttaaaaag tttcaatttc aacgtaaaaa agcaataagc cccaacaatg tgaaagctta
    12961 ctgcttgttt aaattatttt tctattgctt tatcatcact aacaattaaa ggttgatctg
    13021 gtttcacata gaaccacata atcacagcga ttactgctgg aacgattaac aattggaatg
    13081 tcattgtcca acctaaatgt tctacgaata gacctgctaa tagcggactg attaatgcac
    13141 ctatattacc ccataagttc atccaccctg atactgtacc agagaagtta cgtcctaagt
    13201 cagttgctgc agcccaactc atacccattg aaataccgat accaccaaga cctagtgata
    13261 accagaaaat acttacatat aaatttcctg tacgtacagc aaagataatt gaaactgcaa
    13321 acacaataaa tcctgcgatt gcaattacac ctctagctac gaattttgaa cgacctaatc
    13381 ctaatacacg gtcagaaatt gcacctgctg ataagattaa gaagaacatt aataaccaag
    13441 gtaatgaact aatgctcatt tctttaaagt taacgtggaa tacttctgtt aaatacgtcg
    13501 gtaaccaaat taagaataat gtaatgataa attgtacaac aaagtattga attgcaattg
    13561 catagaagct aaaacgttta aagaaatcat tccatggtgg tgatgacttt tcagtagcta
    13621 cgatatcacg attttccata atgaaacgtt tctccgcttc attaaccatt ctatgttgct
    13681 caggtaagtc tttggcaata atcgcccata atacagccat taaaatacct actgcaccaa
    13741 aaatgtaaaa tactgcttgc cagttaaatg cgttaacaat agcaattgta actattggtg
    13801 ctaatacagg tccgaaatat gatcctgcta ataatgcact tgatgctcta cctttttcat
    13861 ttttagagaa ccaaaatgag ttaaacacag cattagaagg gtacattggc gcctcaccta
    13921 caccaaataa gaatctcact aaataaatta aaccgtggtt cttaatcata cccgttaaga
    13981 ttgtaaatgc actccaccaa actaatgcaa ttgtaatcat cttacgtgga ccgaattttt
    14041 cagccaacat ccccgatggt acttgcatta atgcataacc tagagagaag aatgatgcca
    14101 ataagccaaa ttgtggtttg gtcatcccta aatcatccat catttgttta gcgatatatg
    14161 aaatgtttgc tctatccata tacgcaataa ccccaataaa gaagaacgca agcgcgaaca
    14221 tccaccttac gttagttcgt ttttccttca taactattcc tacttccctt attaattttt
    14281 aaaaacttac cctagaacga taaaactttc ggacatctgg catgtccacc cctactttgt
    14341 cacaaaattg taccaaagtt attttttata atattatcag acatggaaag cgtttacaat
    14401 agaatctata aaaattttat taaattccgc ttaacttttg ctattaaaac gatataaagg
    14461 aacttttagt tacatagtag cctatcattt gttaattaaa aatactgcaa tcctagtgtt
    14521 ttaaaacgta tattgcaggc tttcttacat attagacatt cattttaaat tgcataaaac
    14581 tacattgctc attcattgtg cagcgcctgt tatttagatg atcatttgtt gacttttcgt
    14641 acttatagca agcaattttt tgatttattg cttataatac attttaagac aaatgcttgt
    14701 ttcaacatcg catacgacat cattcattat tcatttgaat ttatccagtt tctatttgtc
    14761 tgcaattgct gaaaattaaa ggagcttatt tttaataatg gaacaaatta tcactgaatt
    14821 tattagtcgt tttgggtatg cagccatttt tatattaatt ttattagaaa atgttttacc
    14881 tatcgtacca tcggaaatta ttctgacatt tgctggcctt atgtctgtta aatcacattt
    14941 atcaatttta accttattta ttattgcaac catcgcatcg tttatagggc tgttaatttt
    15001 atattatatc tgtcgtttaa tttcagaaga acgtctatat cgctttattg atcgacacgg
    15061 taagtggatt aaattgaaaa gtaaggattt aaagcgagca aatgattggt ttaaaaagta
    15121 tggcgtatgg gctgtattta tctgtcgttt catacctgta ttacgtgtat tgattaccat
    15181 tccagccggt gtaaaccgca tgaatgttgt gacatttacc gttatttcat taataggtac
    15241 aacaatttgg aatttcggtt taattttact aggacgcacg ttgagcgata gctttggtat
    15301 gttgatgact ggtcttcata catattcacg tattatgtat gttgtcatca ttatcgcagt
    15361 catttacttt gctatacgtt acattggcaa acgtaagaga gtaaaataat attgttgtta
    15421 gtataatggt atattttcaa ttgctataaa taaaaagcag taaaatcatt tcgatggctg
    15481 gaatgattta ctgctttttt aggtttattt ggcatacttt agttgaaagt catctaaaac
    15541 cctttacgtt aagagatata atgaagtttc ccataaaagt tcagttccct cagtatgtac
    15601 catttattcc gcaaacgcat ctcacaatcc atctataata caaactatca tcttaaataa
    15661 gcagaattta ccctgtcttc cacacaaact gttttcgtca ctttaatatg ccttctattc
    15721 aaaaaagtca ctcataacaa attgcgcatt ttaataaatg tagcaatgcc atttgcatgc
    15781 tacaaaaata ctc
//
LOCUS       DAMZLE010000056        15253 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMZLE010000056 DAMZLE010000000
VERSION     DAMZLE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.056"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            319..522
                     /locus_tag="POB92_001499"
     CDS             319..522
                     /locus_tag="POB92_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579005.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            705..1640
                     /locus_tag="POB92_001500"
     CDS             705..1640
                     /locus_tag="POB92_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDI6579006.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPEALDE"
     gene            complement(1721..2689)
                     /locus_tag="POB92_001501"
     CDS             complement(1721..2689)
                     /locus_tag="POB92_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6579007.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2822..3166)
                     /locus_tag="POB92_001502"
     CDS             complement(2822..3166)
                     /locus_tag="POB92_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6579008.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(3208..3618)
                     /locus_tag="POB92_001503"
     CDS             complement(3208..3618)
                     /locus_tag="POB92_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6579009.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(3983..6049)
                     /locus_tag="POB92_001504"
     CDS             complement(3983..6049)
                     /locus_tag="POB92_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDI6579010.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6350..8089)
                     /locus_tag="POB92_001505"
     CDS             complement(6350..8089)
                     /locus_tag="POB92_001505"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDI6579011.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELRALVEAAKIPVVHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQDADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(8136..8825)
                     /locus_tag="POB92_001506"
     CDS             complement(8136..8825)
                     /locus_tag="POB92_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDI6579012.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8818..9213)
                     /gene="cidA"
                     /locus_tag="POB92_001507"
     CDS             complement(8818..9213)
                     /gene="cidA"
                     /locus_tag="POB92_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDI6579013.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9426..10304)
                     /locus_tag="POB92_001508"
     CDS             complement(9426..10304)
                     /locus_tag="POB92_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6579014.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10471..10689
                     /locus_tag="POB92_001509"
     CDS             10471..10689
                     /locus_tag="POB92_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6579015.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            11079..11510
                     /locus_tag="POB92_001510"
     CDS             11079..11510
                     /locus_tag="POB92_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6579016.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(11648..12928)
                     /locus_tag="POB92_001511"
     CDS             complement(11648..12928)
                     /locus_tag="POB92_001511"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDI6579017.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAERILQDLRSQQ"
     gene            13182..14348
                     /locus_tag="POB92_001512"
     CDS             13182..14348
                     /locus_tag="POB92_001512"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDI6579018.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(14518..15039)
                     /locus_tag="POB92_001513"
     CDS             complement(14518..15039)
                     /locus_tag="POB92_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI6579019.1"
                     /translation="MEYKSYYDSPVGRLELVSDGGSLTAVLFENQQGDSTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
BASE COUNT         4749 a         2926 c         2326 g         5252 t
ORIGIN      
        1 gataactttt ttacatctga aagtgagtaa tatttctaaa aatctttaat atttatacac
       61 tttatctttc gaactattta ttttttgttt ttgtgctgtg tttataaaac cgcacactat
      121 attcaaaaat aacttattac acatatattt gcaataagat aattaaagta ggatattatt
      181 tttagttttc tgataggaat gatgatagtc tataggttga atctttactt tttttaaagc
      241 taaatttaca tcaacttaac aatggctggt tatactgaag atgaaaatat ttagtataac
      301 ttagtggagg cgataaaggt gcaattgagt cattccgtta aagttgcaat ttctatctat
      361 ttagcactta tctttataac gttcacttct tatttagtca ttattttata tacgagtatg
      421 actggacatg atgtatcaca tttcgtgtta gatagtcagc attctcatca tggatctctt
      481 acgcaaaaac atttgagtct tcctgaaatc tcatttaaat agtttatcct ctgtgtttca
      541 acattcattt cccatatcga ttcatttatc tatcatctag accactacat cttagatgat
      601 ttttttattt tctcatttca ctctttctta aaagtcgata taatgaatta aatcattatc
      661 atacaccgac atattttatg ttgttggtgt taagttttaa aggggtgaga tacttggcga
      721 ataatcattc agctttgcgt ttaaaaggaa ttatacttgc cattgtcggt gcttgtttat
      781 ggggattagg tggtactgtt tctgatttct tgttcaaata taagaatatt aatgtcgatt
      841 ggtacgtcac tgctcgactt gtagtcagtg gtgttttctt acttattatg tacaaaatga
      901 tgcaacccaa acgttctata tttagcgtat tccaagatcg acgtatgtta ggcaaattac
      961 ttatcttcag tatactgggc atgttagttg tacaatatgc ttatatggca tctattaata
     1021 caggtaatgc tgcgattgca acattactac aatacattgc gccagtttat attattattt
     1081 ggtttgtcat aagaggcgtt gcaaaactaa cattatttga tgtgcttgct attatcatga
     1141 cactattagg aacattttta ttattaacga atggttcatt ttctaattta gtcgtcaatc
     1201 ctgcaagttt attctggggt attttagctg gtgtagcact cgctttttac acaatttatc
     1261 cttcagacct acttaaccgc ttcggttcga ttctaattgt cgggtgggca atgcttattt
     1321 ctggtgttgc gatgaattta cgccatccaa tttggcacat tgatatcact aaatgggaca
     1381 tatcaattat attattttta atctttggta ttatcggtgg taccgcactc gcattttatt
     1441 tctttatcga cagtttacaa tacatatcag cgaaagaaac aacattattc ggaactgttg
     1501 aacctgtcgt agccgttatc gcaagcagtc tatggttaca tgtggcattc aaaccatttc
     1561 aaatcgtagg catcattctt attatgattt taattttatt actatcactt aaaagacaac
     1621 ctgaagcatt agatgaataa gaaaactctg ataatcactt tagcaagtaa ctatttttaa
     1681 caacgtagct accttttatg tgatatcaga gttttttatt ttaattaata atatttttca
     1741 cttggtataa aaagcgtcgg cgctctggta tcggaaatac tggataaata tggaattggt
     1801 aatatccagg tataaagtca tgttcgatac cttttgcact caacaattgc gataagttca
     1861 aagcatctgg gtacagtact tcttttgtgc caacagttaa tgtaatacga cctaaaccat
     1921 ctaagtcacc attaattggt gatactttgt agttatctaa aggtgtgtcc cctgcccatt
     1981 gttcagctaa gaacacactg ccatccacac ctaccattgg gtctttcttt aagtagtcag
     2041 gaatttcagg atgctgcatc gttgcatcta aaactggtga aattaataca atatgtcctg
     2101 gttgcacaat atgtttttct tttaacaatt gagcaaatga taaagcaatt tgaccgcccg
     2161 cagagtcacc cattacaacg atttgtttag aatctgctac ttgattcaat aaatcatggt
     2221 ataacttttc aaaaagcaca tacgtcgctt gataatcttg atgcggaatc ttcggatata
     2281 ctggcatgat gactttagca ttgagtgttt ctgcaagttc atcaataaat tcgaagtgaa
     2341 ttttaagtgg gtcttggaac catgcacctc catgtgcgta taatacaaca cgttgatgct
     2401 tatcttgttt atcattaacg gtataaacgg ttgaaccgta gtgcttatcc actgttactg
     2461 gcatcttaaa ctgataattg gtgccctcat attcacctgc tgtctgtggc gccatttttt
     2521 ctaatgcttt catcgcttct tctttataca taaaacgacg cttagctcta gataatcgta
     2581 taccttgttc taacatataa cttttcacac tgcgtttttc tttaattcta atatgtgcgt
     2641 atgctgctac taaaaatcca aacgcaagtg tagaccattt tttacgcatt tgtcgtcact
     2701 cctctttgat gatttctact atagttctac tgtaccatgc tgattcttat tactaccaat
     2761 atttactggg aaataagtta tgatgtctac ataatcttag caatgctaat catttgcgat
     2821 attaatgctc atcgacatcg ttatttacac tatcattcat ttgcttaaga tgatgttcaa
     2881 aacgttgcat atcgataaaa cgcccttgac gatgcatttc ttttccattc ataaaaatta
     2941 aatccacagg tacagtgaaa atatttaatt ctccagcaat agcttccacc tgactttgat
     3001 taatcacagc taatggcaca ttgggatatg attgcatcaa gtcttcaatt tgtggtaaaa
     3061 cggcatgaca cacgctacac tgatcgcgca tgacatgtac aactgctaac ggatattgat
     3121 gaataaattc acggaatgct tcgacactat ttaattgctt aaccatttcg aacaccttct
     3181 tactttttat attttaaaat gaagtcatta ttttgttgct aaaatgtctt gaaacacttg
     3241 tggcacagag gttatcttct tatcgctcaa tctgtaaaaa ccgcctgtct gtgtaccaat
     3301 acaatgcacg ccatgtgcgg acacaatttc aaactgaaac acccatttca tcttgcttgc
     3361 tttaactaag taaatacgac ctactggttt gtcatgtatt gtgacaggta ctttatactc
     3421 tgcttccgtt ttcataagta taggtgaaat gccttgattg atcattgttg agtaattcat
     3481 gtgctgatta ataaacgctg ttctcaaatc ttcgaaccat ctaatataaa cgatattact
     3541 aacgacaccc atcgcatcaa tgtcgtaacc attcacttcg atttctttct ctgcaactaa
     3601 tagaccttct gttgtcataa tgacaaatct ccttatctta caaatagatt taaaatcatg
     3661 ttaatttatt tttattactt tcatgataaa aatacaagca attgaacttt attacggaaa
     3721 atcaaacttt tcaatgacgt tattaatttg actagtcgtg aagatgcatt tttctaaaat
     3781 gtacaaaaag tacaatccct actttctatc ccccaaacta acatctatgc cattgctata
     3841 catcattgcg cactaaaaag gcagtagctt cattttaagc tactgccttt tgtaacgtat
     3901 tctctttcaa ctcgctactt cgcatacgca ctacgaatga ctaaaagttc attcatttga
     3961 tatgctgttt catttttaat cattatttga ctgtcataat tagcttacct ggatcagcat
     4021 cttgtacatc ttcaatgaca agttctttat tttcaagatt tgtaataatc attggtgtaa
     4081 cgatgcttgg tgcgtgtgct ttcaagtatg ctaaattcac tttcattaat ggttgacctt
     4141 gtgttacttt ttcatcaacg ttaatcagac tttcgaatcc ttcaccattc agtttcactg
     4201 tatcgatacc aatatgaata agtacttcga cgccactttc agattctaat cctatcgcat
     4261 gtttcgtagg gaagattgtt ttcactgtac catcaaacgg tgctacaatt tcaccttttt
     4321 cagggataaa gccaacacca tcacccatca ttttaccagc gaatacttga tcaggtactt
     4381 ctgataatgg aatgatttga ccgatacctg gtgcatagat gtctgttgtt ccaagtgctt
     4441 ctacgtgaac tggttcatct gacatttctt cagtcactgt cgtttcactt ggtttcgtaa
     4501 tttcaccact cataatcttg gccatatcat gtttaatttg atctgatttt ggaccaaaga
     4561 tagcttgcat attgtttcca acttctaata cacctgatgc gcctaaagct ttaatacctg
     4621 ctacatctac ttttgattta tcaaccactt ctacgcgtag acgtgtaata catgcatcta
     4681 aatgtttaat gttttctttt ccacccattg catctaagac atcaaatggt aattttgcga
     4741 cactagagtt acgaatttca gtttcttcat cttcacgacc tggtgttttc aatttaaact
     4801 tacgaattgc aaagtcgaat aagaagtaat acacgatagc atatacaata ccgactggaa
     4861 taactaataa tgcgtgtgaa cgatcccagt ttaataaacc atataaaata taatctatga
     4921 aaccacctga gaatgtcata ccaattttaa cgcctaataa atgcattact aagaatgatg
     4981 taccagctaa taatacgtga attccataaa gtactggtgc tacaaataag aatgaaaatt
     5041 ctaatggctc agtgatacca gttaaaaatg cagttaatcc tgctgataac attaaaccac
     5101 ccacgacttt tttacgttct ggtcgtgcat ttttataaat agcaaatgcc gccgctggta
     5161 aaccaaacat cataaatgga tatttaccag tagtaaatgc accagcagta aatggtacgc
     5221 catctttcaa ttgtgccatc caaatacgtt ggtcaccacg aaccaattca cctgcgtgat
     5281 ttgtataact tccgaattca aaccagaacg gtgaatagaa aatatgatgt aaaccaaatg
     5341 gaattaatga gcgttcaata ataccgaata taaacgttgt taatgttaaa tttttattta
     5401 ataagaaatt cgataaacta tttaatccat cttgaattgg tggccacgca aagcttaaaa
     5461 gcacacctgt tgcgattgct acgaccgatg tcacaatcgg tacaaatcgt ttacctgcaa
     5521 agaatcctaa aaatggtggt agtgtaatat tataaaattt gttgtaacac catgcggcta
     5581 aagcacccat gataatacca ccaaacacac ccgtttgtaa cgttggaata cctaatacta
     5641 aagcatgtgc tggttctttc gctgcttgac ttaattcttt tgcccctttg gcatatgaga
     5701 aaatgtcatc aattgtaatg tgcaacactt tccccattgt tgcattcata attaagtaac
     5761 ctactagcgc tgctaatgct gcaacaccgt ctcctcctgc taatccaagt gctgtaccaa
     5821 ctgcaaataa taatggcaag ttatcaaata caacttgtcc tgctgcttcc atgaccgacg
     5881 aaatcattac aatgatatcg ttttttaacc atggtgcaat ttctactaat tgttcgttgt
     5941 gcattgcgtt accaaacgct aataaaatac cagctgctgg taaaatcgca acaggtaaca
     6001 ttaatgcttt accgatacgt tgcaattgtc caaacaattt cttaaacata taaacatccc
     6061 ctatctcaat ttccgcttcc atgtacaaaa aaagcaggca tgaacaaaca agactattct
     6121 catagtgcgg ttgtttgctc atgcctgcat taccagtaac acgcaagtga tatctaattc
     6181 atagctatta tacatatttg taaaagcgtt ttcaacaact ttttcacaat tttatcatca
     6241 attcagtacg atcatttgtg ataaataaac aaaacggcaa ttcccatttt gttgaaatag
     6301 aaattaccgt ttttatagtt caattcaatt gtgataacct ttaaatcagt tataagaaac
     6361 gttttgctgc cactgaaatt ggtggaattt gatctaaatc taaatgctta tcttcagtaa
     6421 ttgatctaaa tgcccactta ccataaccaa gcgcttcttc atttacaatt ttacctggta
     6481 atggcgcagc attaggatca acatatacat ctacaatcgt tggtacatct tgtgctaatg
     6541 cctcttcgac tatagcatct acttcgctag cactcttaat tgtataacct ttaccacctg
     6601 ctgcctcagc aaattttgca tgatccatat cagaaaaatc aactgcatat tctaattcac
     6661 cagctgcttg ttgttcatat ttaataaatg ctaactgttt gttattaagt acaaatacag
     6721 ttaaaggtaa atcatattgt actgctgtag cgaagtcttg cattaccatt tggaatgcac
     6781 cgtcaccagc aatggcaatc gcttgtctat ttggatatgc aatttttgat gccatagctc
     6841 ctggaagacc gcaacccatt gtacctaacc aacttgaaat gatgaacttg ttgttcacac
     6901 caagatttaa ataacgtgta gaccaaactg ttgcagtacc aacatctgct gaaattactg
     6961 catcatcttt aataaatttg ttaattgatg ccattaaacg ttctggacgt aatggtttgc
     7021 tattattatt tttatcttgt tccatccatt tatcccaaac cgctttacgt tctaacgttt
     7081 tgtttaaaaa tggtctttcc gcaacatgtt taatattttc agttaactga tgcaatgcaa
     7141 ttttactgtc tccaacaatt cctacattaa tattaaaacg atgtccgata tttttaggat
     7201 ttgtgtcgat ttggattgct ttaatatttt tcttaggtaa gtaatctaca tatggatagt
     7261 ttgtacctac cataattagt aaatctgcat cctgcattgt ctgatatgag gtttttgtac
     7321 ctattttacc taagttacca atactgtatg gatggtcatc cggtaagatt gtcttagcag
     7381 gtaatgaatg aacgaccgga atttttgccg cttcaacaag cgcacgtagc tcatctttcg
     7441 catgttttgc accaacacca atcaacataa ccggcttttt actcttattg attaatttaa
     7501 ccgctttttt aatgtcttta tattttggtg atacaacagt tggtcttgat gtatctactg
     7561 gtttattcgt tgtatcttta attttttcag ttaataagtc gttaggacaa ataacaacag
     7621 ctacaccttt ttgttcatat gccgtacgaa ttgcttcgtt aacaatttca aacacattgt
     7681 cacctttttc aatttggtga ttataaacgg ctacatcttc acataatttt tgtaaatttg
     7741 tttcttggaa tgctttcgtt ccaagtgctg tactattcgt ttgtccagat aatattaatt
     7801 gcggtacatt atccattttg gcatcataca taccatttaa taaatgaatt aaaccagggc
     7861 caccgatact taatgccaca ccgattttac cagttaattt tgtgtaacca gcagccgcta
     7921 agcttgctac ttcttcatga cgtacatgat aaaatttaaa ttgatctctc actgtacgta
     7981 aactatcgac tactgcgtcg attgagtctc ctggaatacc atacaagtga tctatatccc
     8041 atgcttgtaa tgctttaact aatgcttcat ttgcttttat ttttgccatt attactaata
     8101 gcctcccttt ctgtctatca ttttaaatat ctaaattaat agaataataa aattagaaca
     8161 ggtattaaca ctgaactaat aactgcagtt aaaatcatcc caattgaact aaatgcaccg
     8221 gattcaatat ccatttctag tgctttagct gtgccaaatg catgtgacgc attcccatac
     8281 gttaaacctt tggcgataga agattcaaat cttccaaatc ttaataacat cgaacctaaa
     8341 atactaccga ttaaacccgt tgtgataata aaaagtacgg tcatcgtatc tgtaccacct
     8401 agttcatgtg acacttcgat acctactgcg gctgttatag atcggggcaa taacgttaca
     8461 atgacgtctt tagaatagcc aaatgcttta agtgttaaga acactaacat gaaattcagc
     8521 atgacgccag ttaatacact tgcaaaaatg atagagacat tgtctttaat tttctctcta
     8581 tttttataaa gtgggtacgc taaacatacg accgttgcgt ttaaaatatg gttgatccaa
     8641 ctgccacctt tcatataccc gttataacta attccaaaga taagtaagac aaaaataatt
     8701 cctaaagatg caattaatgc tggattcaaa aatgggttcg gatatttttg ttgtaacctt
     8761 ttagcgaaat aatataaaac gacagtcaac aaaatcatta ataaggcttg cacgtaatca
     8821 ttcataagcg tctacacctt tacgatgttt atgtttaaca gacatttttt cagcaatata
     8881 acctgaagat aatgcaacga tacatgttcc tatgataatg actgcgaaaa agagtatata
     8941 atttagcgta atttcggaag caacatccat aatacctaca actgacggta tgaagaaaaa
     9001 gaccatcgtc tttaataaaa agtttgcacc gtcttctacc caagttagcg gtacaatctt
     9061 aaattgtaat agtaaataaa ataaaaatag accaacaata ctgccggcta agggaagatg
     9121 aaaaatcttt tgaatttctg tgccaatata agtaatcaca atgatgattc ctagttgtag
     9181 tagtagtttg attattaatt ggactttgtg catggcgcca tccctttcta aatatgtcta
     9241 aattgttaca ataactatta taaagatggc gtacacaata taaaaatact ctttttttat
     9301 agtttgtatt actattttct atggaaacgc tctctaaatt tcacttcatt ttcacaaaaa
     9361 aacactcatt atttcaatga cccacgtaat gatgcgcctt aaataatgag tgctttatta
     9421 atatgttagc ctaaacggtc tttcaaaaat tctatccatt tcgttgtggc atgacttaat
     9481 tgtttatcct tcttccaaac aacacctaat tcccaatgta catgagcatc ttcaatgcgt
     9541 agcagcttca catctccatt tagttgctct gaaattgatg ttggtaaaat actaatacct
     9601 aattcattcg taactaaatc ttctataaca tgccattgtg aaatttgcgc tacagtattc
     9661 ggaacaaagc caacgttttt agcattttca ataattttat cattcaagta aaagtcttta
     9721 ttaaataaaa tgaagtcttc acctgctaaa tcttcgagtt taacagtttc atattttgcg
     9781 agtcgatgct ctctgctcac gataagtcgc aaatcttcct tatctagggt agtataatcg
     9841 aaaatatgat gatcgactgg caaagtggtc acgcctatat ctacttcatc attgataatt
     9901 tgctgttcaa ttgttttacc gccattttct atcaaattat atgtaacatt tggatattgt
     9961 tgatggaatg caccaagaat attgataaga atcttcatat tcatgactgc cgacatgccc
    10021 atgtttatat gtcctgtttc cagtccattc aagcgttcca tttcagatgg taaataatca
    10081 tacagtgcaa caatttcttt acttttctca taaaaaattt gacctgcatc ggtaagaatt
    10141 aaatgtcttt tacttctatc aaataagggc gtgcccattt cattttcaat atctttaatt
    10201 gctttactaa ttgtaggttg tgcaatatat aatgatttgg aagcattagt catgcctcct
    10261 tgcttaacga cttcaataaa atatttcata tgtttgatat ccactaacat catcctaact
    10321 atttcaacac gtttatttca ttatacacaa cttcttaaac attcaataaa tttagattta
    10381 taaaattgct ttataaaaag ttttatgtaa aatacacatt cttttatggt attatattaa
    10441 tggctcaagt atattaatcg aggtgaggta ttgagctttt acgaatttat gcaaagtttt
    10501 cttggtgatg acacaccatt aggtgaattg gttgattgga ttaatcaaga tagcaatttc
    10561 cctagagaag tgaagagcca tagtgaaata ttgtcatatt ttcgaacaaa tccatgtcct
    10621 gaaagcatct cagtacctgt aattaaacgg gcactatcag ttttcaacca attcacaaat
    10681 gtacaatgat acatgaatac atggagtcac atcatctcat gttagcgctc tgattgcagg
    10741 gcgctatttt taatgttatt gctacattta gtatattgtg attgctattt tctttcagtt
    10801 atagaaccac gtaccttttc ttttaaatca ttttatattt tcccactaat atccgctgtt
    10861 aatcaatcct gacatccttg tatcactatg acaattaatt gttaaataca tgaatttcta
    10921 cattttatga aaaaatccat ttttattaca attcaacact ttatatgaca acttcattac
    10981 agttactttt attgttgatt gcttacattg ttttctaaaa aaaatttgtt atcataatta
    11041 acgttgaata aagaaaaaaa ttaacttggg agataaaaat ggaatataaa aagatactaa
    11101 ttcgtttatt aattgctttt gcagtacttt tctcagcaga tttcacttat caatccgtcg
    11161 aacaaacgca tcaatcgcat gccgcagtta attattatag taaaaaccaa tgtacatggt
    11221 gggcatttaa acgtcgcgca caagtcggta aacctgtttc taatagatgg ggcaatgcta
    11281 aaaattggta ttacaatgca cgtaaatcaa aatatgcgac tggtcgtaca ccaagaaaat
    11341 ttgctgtcat gcaatcaact gcaggatatt atggacatgt cgcagttgtt gaacaagtat
    11401 ataaaaacgg tagtattaaa gtttcagaat acaactttta tcgcccatta aaatacaata
    11461 cacgtgtact aagcaaaaag gcagcacgta actttaacta tatttactaa tcaaaaaact
    11521 tctatcacga acgcttcaat ttcctgtatg cgtgtgatag aagtttttat ttttgttcat
    11581 attaatttat ctaagcgcta cgatggactg acctaaactt ttctttaaaa tcgctatttg
    11641 caatttacta ttgttggctt cttaaatctt gtaaaatacg ttccgctgct tgtgtatttg
    11701 cacggggttc ttttttcaaa gcttcagcta ctttagcaat ttcatcacct tttgctccta
    11761 caacaatagc taatgattta tattgtaaac tcatatgacc ttgttgaata ccttctgaca
    11821 caagcgcgcg acatgctgca aagttttgcg ctaaaccaac ggcagcaact acatgaccta
    11881 attcttgtgc tgactctaca tttagtagct ctaatgaagc tttagctatt ggtaatactt
    11941 tcgtaccacc cccaacaatt gccaatgtca taggcacttc aattgtacca atcaatcgtt
    12001 gacgatcttg atcgtaacgc catgtagcaa taccacgata ctgtccgtca cgacttgcgt
    12061 atgcatgcgc acttgcttct gcaccacgcg tatcatttcc tgttgctaaa acaacagcat
    12121 gtatgccatt cataacacct ttattatgtg ttgctgcacg atgaatatct acttgtgcca
    12181 atacagaagc acgttccatt cgtttggcaa cctcttctcc agttctctcg ccccttgcta
    12241 aatctttaac atcaatttcg ccttgaactt taacaacgga cgctgttgca tgattggata
    12301 aaatactcat taaaatgtcg ctttgcggaa attcattttt taaaaatgca gttatggcct
    12361 ctaaaatcgt attaagcata ttagcgccca tagcatcttt cgtatcaaca aatactttta
    12421 aagatagtaa ctgttgctca ggaaatgtat caatcgctat acgttggtaa ccaccaccac
    12481 gcgctttaat agaaggatat gcctcatccg caattttatg aatttgcttt tctaaagctt
    12541 taatgtctgc tgataatttt tcagtatcgt caacgccatc aaagacgatt tgacctatca
    12601 taatacgttc agaagatacc gttttaaatc cgccagtctg attcactagc tttgcaccat
    12661 aactagctgc agcgacaact gaaggctctt ccaccatcat aggtacaaca tatgccttat
    12721 cgtccacaat gatattcggt aataatccaa cgggtaatgc accttgcgcg atgacatttt
    12781 caattaaact attggctact tcttcatcga ttaatggatg attcagtaaa atgtcgaatt
    12841 gttcttctga taaccattgc ttatcaacca attgttgtaa cttttcttta cgagataaat
    12901 gtcgaaaatt cttatctaaa ttttgcatgg acgtactcct tttacttcac ataattttta
    12961 acatttcaat cactactatt tttaccacaa aataacgtca ttcgtcttaa aattcaattg
    13021 aataattgtc gttttgactt taaaataaaa caaggtaaat taaaacgctt acaagaaacg
    13081 acaaatcatt tttaaattta gtatatttct ttgtataaaa ttagcatatt ctgatatgat
    13141 acaagtgttg cttttataaa tttgaaagga tgtaaaacct tatgacaata ggtatcgata
    13201 aaataaactt ttacgttcca aagtactatg tagacatggc taaattagca gaagcacgcc
    13261 aagtagatcc aaacaaattt ttaattggta ttggtcaaac tgaaatggct gttagtcctg
    13321 taaaccaaga catcgtttca atgggcgcta atgctgctaa ggacattata acagacgaag
    13381 acaaaaagaa aattggtatg gtaattgtgg caactgaatc agcagttgat gctgctaaag
    13441 cagccgctgt tcaaattcac aacttattag gtattcaacc ttttgcacgt tgctttgaaa
    13501 tgaaagaagc ttgttatgct gcaacaccag caattcaatt agctaaagat tatttagcaa
    13561 ctagaccgaa tgaaaaagta ttagttattg ctacagatac agcacgttat ggattgaatt
    13621 caggcggcga gccaacacaa ggtgctggcg cagttgcgat ggttattgca cataatccaa
    13681 gcattttggc attaaatgaa gatgctgttg cttacactga agacgtttat gatttctggc
    13741 gtccaactgg acataaatat ccattagttg atggtgcatt atctaaagat gcttatatcc
    13801 gctcattcca acaaagctgg aatgaatacg caaaacgtca aggtaagtcg ctagctgact
    13861 tcgcatctct atgcttccat gttccattta caaaaatggg taaaaaggca ttagagtcaa
    13921 tcattgataa cgctgatgaa acaactcaag agcgtttacg ttcaggatat gaagatgctg
    13981 tagattataa ccgttatgtc ggtaatattt atactggatc attatattta agcctaatat
    14041 cattacttga aaatcgtgat ttacaagctg gtgaaacaat cggtttattc agttatggct
    14101 caggttcagt tggtgaattt tatagtgcga cattagttga aggctacaaa gatcatttag
    14161 atcaagctgc acataaagca ttattaaata accgtactga agtatctgtt gatgcatatg
    14221 aaacattctt caaacgtttt gatgacgttg aatttgacga agaacaagat gctgttcatg
    14281 aagatcgtca tattttctac ttatcaaata ttgaaaataa cgttcgcgaa tatcacagac
    14341 cagagtaatc tgtgtatttg aaagcattta aatataacct aaaaacagca gtaagacagc
    14401 ttctcattga aatcgtctta ctgctgttct ctatttataa cacttcgtat tgaatgaatt
    14461 agctaagcct gtttgacaca ttattgaagt tttcctaatg cctggatcct ttatacgtta
    14521 cggcttcgtg ctatgttttg gtacataaag ctttgacata tcgatattct ccaactctaa
    14581 cagcttaatt ttattattaa tcgttccacc gaaccctgtt aagctacccg ttttaccgac
    14641 aacacgatga catggcacga taatagataa tggattactt ccgactgcac ctccaaccgc
    14701 ttgggctgac atttttggct tgttaagcag cttgcctact tttttggcaa tagcaccata
    14761 cgttgttaga gtcccataag gaacctgtct taattcattc caaacacact gttgaaaatg
    14821 actacctgtt ggctttaaag gtattgtgat ttcaggattg tcacctttaa aatacgcgtc
    14881 taaccactgt gtcgcctctc taaatatcgc taaagacgta ttttcttccc tagtactatc
    14941 accttgttga ttttcaaaca aaacagcggt cagacttccc ccatcactca caagttccaa
    15001 tcgtcctaca ggcgaatcat agtaactctt atactccata aaaatgcccc ctttttctaa
    15061 tcatacgcct taattgttaa tttagaaaat attcatgttc acatttttta aaaataaata
    15121 agtcgtcaat tacgtatata aacacgtaat accagctatc actttgctgc aatatacagt
    15181 tacatatctt actacacgcg ctaacctctt actttgtaaa ccaaatctta aattaaaata
    15241 ttgaaaatgc aat
//
LOCUS       DAMZLE010000057        15035 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMZLE010000057 DAMZLE010000000
VERSION     DAMZLE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.057"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            250..3323
                     /gene="mupA"
                     /locus_tag="POB92_001514"
                     /pseudo
     CDS             250..3323
                     /gene="mupA"
                     /locus_tag="POB92_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
     gene            3393..4280
                     /locus_tag="POB92_001515"
     CDS             3393..4280
                     /locus_tag="POB92_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6579020.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLG
                     KIVANMHKNINSLNLFGFSHNFNGGTIEFTNKWTSSWGDLFIKSRMDKLCSEIYKKRL
                     FSISDLLLYEDIRKIMLKSLYYHQSSPSLLHGDLWKGNILFQKNGDPILCDPVCLYGD
                     REFDIGYTLALEKFPLDFYQEYNNIYPLCVGYEKRIEFYKLYVFMIHLFAIGTDFYDK
                     VKDTMYNILNNYGVDFIKG"
     gene            4625..4918
                     /locus_tag="POB92_001516"
     CDS             4625..4918
                     /locus_tag="POB92_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6579021.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            4993..6927
                     /locus_tag="POB92_001517"
     CDS             4993..6927
                     /locus_tag="POB92_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6579022.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL
                     LQIAYIKYLEKYFKDLMREI"
     gene            6931..7242
                     /locus_tag="POB92_001518"
     CDS             6931..7242
                     /locus_tag="POB92_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6579023.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            7254..7880
                     /locus_tag="POB92_001519"
     CDS             7254..7880
                     /locus_tag="POB92_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579024.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
     gene            8118..8508
                     /locus_tag="POB92_001520"
                     /pseudo
     CDS             8118..8508
                     /locus_tag="POB92_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            8448..8747
                     /locus_tag="POB92_001521"
     CDS             8448..8747
                     /locus_tag="POB92_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDI6579025.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVVPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSRYKQKTEYHKQMHMR"
     gene            8841..9386
                     /locus_tag="POB92_001522"
     CDS             8841..9386
                     /locus_tag="POB92_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDI6579026.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMIL
                     DDYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQEREIIFKEDREDGYTL"
     gene            9770..10630
                     /locus_tag="POB92_001523"
     CDS             9770..10630
                     /locus_tag="POB92_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6579027.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            10717..11058
                     /locus_tag="POB92_001524"
     CDS             10717..11058
                     /locus_tag="POB92_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6579028.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(11297..11368)
                     /locus_tag="POB92_001525"
     CDS             complement(11297..11368)
                     /locus_tag="POB92_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6579029.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
     gene            11517..11702
                     /locus_tag="POB92_001526"
     CDS             11517..11702
                     /locus_tag="POB92_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579030.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHI
                     IRVKDGLLGWRKEIVTE"
     gene            complement(11921..12664)
                     /locus_tag="POB92_001527"
     CDS             complement(11921..12664)
                     /locus_tag="POB92_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDI6579031.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NARQNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSA
                     NPNDNDSDIEEKSEETEAKKGFWSRLFGN"
     gene            13075..13751
                     /locus_tag="POB92_001528"
                     /pseudo
     CDS             13075..13751
                     /locus_tag="POB92_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT         5734 a         1891 c         2341 g         5069 t
ORIGIN      
        1 aatttataat agtatagttt caacatggga tgtttataaa aataacgaat atagcaaaga
       61 cctttcaaga gaattaaaat ttaaattata tactactaat gatataattt gcagtagttt
      121 gtattataac tttactttat ccaatatatc tttctatgga gacatgaaag gaaatgaatt
      181 tcatgaaaat tcaccaagac taatagtaaa aataacgaaa taagtgatac tctaggaggc
      241 tgaaaagttt tgacaaagaa atatttaaac acccagaatg aaatatcagc attttggaat
      301 actcaaaaga tatttaaaaa atcaattgac aatagaaaag gacaggaaag ttttgttttt
      361 tatgacggcc ccccaactgc aaatggcctt cctcatgctg gccatgttct tggaagagta
      421 atcaaggatt tagttgcaag attaaaaact atgcaaggtt tttatgtaga aagaaaagca
      481 ggatgggata cccatggctt accagttgaa ttagaggttg aaaaaaaatt ggaattaaag
      541 gaaaacaaga cattgaaaag tatggaatag aaaattttat aaatgaatgt aaaaaaagtg
      601 tatttaatta tgaaaaagaa tggcgggatt tttctaaaga tttaggatac tgggttgaca
      661 tggactcccc ctatataact cttgagaata attatattga aagtgtatgg aatatattat
      721 ctacattcca taaaaaagga ctattatata agggacataa ggtgactcct tattgtacac
      781 atgatcaaac cgctttaagt tctcatgaag tagcgcaagg ctataaaaac gttaaagatt
      841 tatcagctgt tgttaaattt caacttacaa atagtaaaga tacttatttc ttaagttgga
      901 ctaccactcc ctggactttg cctgcaaatg tagcattagc tataaataaa gatcttaatt
      961 attcaaaaat tcgggtagaa aatgagtatt atatcttagc tacagatcta attaattcta
     1021 taataactga aaaatacgaa attattgata ccttttcagg aagtaattta attaatttaa
     1081 aatacattcc tccttttgaa agcgacggtt tagttaatgc atattacgtt gttgatggag
     1141 aatttgttac taactcagaa ggaactggta ttgttcatat agcaccagct catggggaag
     1201 atgactacca attggtttta gagcgtgatt tggatttctt aaatgttata acaagagaag
     1261 gagtatataa tgataggttc cctgaattag ttggtaataa agctaaaaat agtgatatag
     1321 aaatcataaa attattatcc aaaaaacaac ttttatataa aaaacaaaaa tatgagcata
     1381 attatcctca ttgttggaga tgtggtaatc ctttgatata ttatgcgatg gaaggttggt
     1441 ttattaaaac aactaatttt aagaatgaaa ttattaacaa taataataat atagagtggt
     1501 ttccttctca tattaaggaa gggagaatgg gaaatttctt agaaaatatg gttgattgga
     1561 acattggtag aaatagatat tggggaacac cattaaatgt atggatttgc aatgattgta
     1621 atcacgaata cgcaccaagt agtattaagg atttacaaaa taattccatc aataaaattg
     1681 atgaagatat tgagttgcat agaccttatg ttgataatat cactcttagt tgccctaagt
     1741 gtaatgggaa aatgtctcga gtagaagaag taatcgatgt ttggtttgat agcggctcta
     1801 tgccgtttgc tcagcatcat tatccttttg ataaccagaa aatttttaat caacactttc
     1861 cagctgattt tattgcagaa ggagttgatc aaacgagagg ctggttttac agtttactag
     1921 taatttctac tattctaaaa ggaaaatctt cttataaacg tgctttatct ttaggacata
     1981 ttctagacag taatggtaaa aaaatgtcta aaagtaaagg aaacgttatt aatccaactg
     2041 aattaattaa taagtacgga gccgattctt taagatgggc cttaatttcg gatagtgctc
     2101 catggaataa caaaagattc tcagaaaata tagtagctca gaccaaatcg aaatttatag
     2161 atacgcttga taatatttat aaattttata atatgtataa taaaatagat cactataatc
     2221 ctaataatga aattacaaaa agtagaaata cattagataa ttgggctctt tctcgcttaa
     2281 acaccttaat aaaagaaagt aatatttatg taaataatta cgatttcact tccgcagcca
     2341 gattaattaa cgaatatacc aatacaataa gtaattggta tattcggaga tcgagaggac
     2401 gattttggga acaaggaatt tctaacgata aaaaagatgc gtacaatacg ctttatgaaa
     2461 ttttaacaac tttatcaaga ctagtggctc catttgttcc atttatatct gaaaaaatcc
     2521 attataattt gactggaaaa agtgtgcatt tacaagatta tccacaatat aaagaaagtt
     2581 ttattaatca agcattggaa gatgaaatgc ataccgttat aaaaattgta gaattatcta
     2641 gacaggctcg caaaaatgca gatttaaaaa ttaagcaacc tttatcgaaa atggtgatta
     2701 aacctaatag tcaattaaac ttaagttttt tacctaatta ctattcaata ataaaagacg
     2761 aattaaatat aaaaaacatt gaattaactg ataatattaa tgactatatt acctatgagc
     2821 ttaaattgaa tttttcttct gtgggaccaa aactagggaa caaaacgaaa aatattcaaa
     2881 cattgataga ctccctatca gagtatgata aaaaaagttt aattgagtct aataacttca
     2941 aaagtttatc ttctgatgct gagttaacta aggatgattt tataattaaa accttaccta
     3001 aggatagtta tcaactcagt gaagataatg actgcgttat attattagat aaaaatttat
     3061 ctcctgaatt aattcgcgaa ggacatgcta gagagctcat tagattaatt caacaattaa
     3121 gaaaaaagaa aaatttacca ataaatcaac gtattgatat ttatatcggt gtaactgggg
     3181 aattattaga atcaataaaa accaataaaa atatgtttaa agaaaatttg ctgattaaaa
     3241 atatacactt aaatgttata gatgaatatg aaaatactat tcattttaat aataaagaaa
     3301 taaaaatttc cttattatat taaacaaaca tggccactct attttagtag agtggccatg
     3361 agttatcaaa gccaagcaaa aggtgataaa ctatgtataa ttttgattat tctaaattac
     3421 caattaaaaa tattcaaaaa atatttccta ttgcaggggg atatgtgaat ctatcttttt
     3481 cagttgatgc ttccaacaaa aaatattttt taaagctaca acctaatact aaatcaaatt
     3541 tttttgatta tgagttatcc agtttaaaag aattaactga taaaaatatt cctgttcctc
     3601 aaattattaa taaaggagaa ttagacaata attctttttt actgttagag ttcattgaaa
     3661 atggccatgc ttatcctgaa agctatagaa aactaggtaa aatagttgca aacatgcata
     3721 aaaatataaa ttcactaaat ctatttggct ttagtcataa tttcaatgga ggaacgattg
     3781 aattcactaa taaatggact tcttcttggg gggatttatt tattaaaagt aggatggata
     3841 agctatgttc tgaaatatac aagaaacgtt tattttcaat ttcggatctt ttattatatg
     3901 aagatatacg aaaaattatg ttgaagagct tatattatca ccaatcgtca ccatcattac
     3961 ttcatggtga tctttggaaa ggaaatattc tttttcaaaa aaatggagat cctattctat
     4021 gtgatccagt ctgtttatat ggagatagag aatttgatat tggatatact ctagcattag
     4081 aaaaatttcc attagatttt tatcaagaat ataataatat atatccgttg tgtgttggat
     4141 atgaaaagcg aatagaattt tacaagttat atgtgtttat gattcattta tttgctatag
     4201 gtacagattt ttatgataaa gttaaagata ccatgtataa tattttaaat aattacgggg
     4261 ttgattttat aaaaggatag ggtgttgact ttgtttcaat tttctaaaat catttatcta
     4321 cttctaatag tcactttttt cgaacgttta taacgcaatc atgatgagag tattaataca
     4381 gttaattatt tgaaatactt tagtttatat aatgttgaat tataggtagt gaaattgtat
     4441 ttattataca ttgtcatatc gttgtttaat gatagttaat ttaaggataa tagaattaat
     4501 attatatata gatgaacaaa acaagcaaca gagcagaaaa aacactttta gattattttt
     4561 actcattctc tattttattt atttttgcta taataaataa ataatattaa atggaggtgt
     4621 ttttatgaaa aagaaatttg tttctagttg tattgctagt acaatcttat tcggcacttt
     4681 attaggtgta acttataaag ctgaagctgc aacagtacat gtagctggtg gtgtttggag
     4741 ccatggtatt ggaaaacatt acgtatggtc ttattatagt cacaataaaa gaaatcatgg
     4801 ttcaacagca gtagggaaat attcatcttt tagtggtgtt gctcgacctg gtgttcaatc
     4861 taaggcatct gctccaaagg cttggggtgg aaacaaaaca ttttatagtc ttcactaatt
     4921 acatatatta attaaggaga atagacagaa ttaagtaaac ttaattctgt ctattctctt
     4981 gaaagaggca atatgaaaaa attcaaatta tttttagatt ttagtacttt attattaatt
     5041 tcagggctat tatttttatt tttcttcaaa gaaaatgaag aaattatacc tgaaagttcc
     5101 aatattttaa caatctctaa ttgggataaa tctaacagta aatcaaaagt gttagatgtc
     5161 attgaatcag gcgccaaaaa tcaaaatata caaataatta aatccgttaa agattttgat
     5221 aataaaaaag aattttttgt tttcaactca aaacgaaata attctgactt tatacgtaat
     5281 aaaacatcac ttttaactcc atcagattta ttaaacagag agataaaagg aaagtattat
     5341 attattggtg aacatttcaa tgtagaagag cttacgagtg aattaaaaaa agctggttta
     5401 acatctgaag ttgaatacat aaatagaaac atattatttt ttgaattagt catagataat
     5461 gatttattag tgcctttcat attcttaggc gtattatatc ttttgtactt cttatatgac
     5521 agaggatata atttgaaatt ttatgctatt caaaagctac atggcttttc aactacacgc
     5581 attatttttc ataatattca tataaaaatc atttattggc tagtacttac cgctattttc
     5641 tttattgcta atatattaat cattcatata gccaatttag aattggattt cttaatgttt
     5701 attcgtagat ttatcgtgtt gctttttgtt tttgcttgta ttactacatt gctgtggctg
     5761 atttcgtata gtcttctttt gaagttaagt attccattaa tcttaaaagg gaaaaaagga
     5821 tataagtttt caatatttat gggtaccttc accaaatgta tcatggtgct aatactatct
     5881 tttttactgg cacaaaattt aaatgcttat acgaagttaa agaatatata tgactctgaa
     5941 aaaatctggc aaaagttaga taattactat acattagaga tttctcctaa tattagaaat
     6001 tcagaagaaa aacaaatttt agaaactaat atatacaatt taattaagaa aagcgaacaa
     6061 cttggtggat tgcttttaaa gaataataat atagccaatc ccgataaaaa caattacatt
     6121 cctgaaaatg gcaatgtctt ttttatcaat aacaattttc tgaatttcta taaaaatata
     6181 aatcatgatt ttaaaatgat tgacaatcat tcggataaaa taaacgtgtt tattcctcca
     6241 aggttacaat atcaaaaatc tgaaattcaa aaaaatcacc aaggatggat tgactttcaa
     6301 aagcaacaaa acaaaaaagt agatgtgcaa gtactgtcaa aaaacgtatc tgttttttct
     6361 tttgatcgca caactaattt gaaatttggg tatctagatt ctccaatagt aatgttgttg
     6421 acacttgacg atttaacagg agatttttat ttagctgctg tttctcaagg tggatatcta
     6481 tttaaagatt tagatacact taaacagttg cttaaagata atcacctaga agaagaaatt
     6541 agcggtataa ctaattacaa ggattctgtg ttaaatgaat taaatgaaac gaaaacaaaa
     6601 atgataataa caatagttac tattattatt aacgttttca ttcttttgat agctactgta
     6661 tttgaaacaa ttcaatattt tagctggaat aaaaagcaac tattattaag aaaaattcat
     6721 ggttattcat tattcagcag taatgtacgt tatttaacaa tttctatctt actttctatt
     6781 atgttagctt acactacgca tattttattt ggttcaaaga tattactatt cataataatg
     6841 tctattgcta ttattcaaca tttacttcaa attgcttata ttaaatatct tgaaaaatac
     6901 tttaaagatt taatgaggga gatttaaaca atgaaaagaa ctttaattat tgtaacaatt
     6961 gtactattaa tcatcatact tagtctgtta ttggttagaa atgagcattt agatcgtttt
     7021 aacccactgt taaaagaaaa agtatcttat gccaaagttc caaaagatac ccaggactat
     7081 agaaatataa ctgtatactc taaaaatgga gaaaaaaaga gttataaatt ggatttttta
     7141 ggttatgatc ctacaaaaga atatgtgaaa gtaaatcata aagggaaata tgtgcgttcg
     7201 atagaataca ttacaaaaga tattcctagc tttttaaaat gaggtgaccg atagtgataa
     7261 aacttgaaaa tgtttttgta aaaaaaggta ataaaaacat tttagacgga tgtaacttta
     7321 attttgaaaa aggaaaatca tatgctttag ttggagaaag tggcgctgga aaatctactt
     7381 tacttaacat aatcgctggg ttcgaagatg tatcacaagg aagcatatat attgaagata
     7441 agttactaaa aaagaaagtc gatttttata gatatacgtt agggtatttg tttcaaaatt
     7501 ttgctttact tgaaaatcaa acaatcagtc aaaatttaga tttagcccta aagttcaaaa
     7561 agaataaaaa agataatatg aacttaaaga aagaagtatt aaaaaaggtc ggcctagatt
     7621 tagatatcaa gagaattgtt agttctctaa gcggtggtga acaacaaaga gtagcattgg
     7681 caagattaat attaaaagat ccgaaaatca ttttagctga tgaacctact gggtcactag
     7741 atacgaaaaa tggaaaaata gttattgatt tattattgaa gctacttgat gaaaataaaa
     7801 ctatgattgt cgtaacgcat gatttagagc ttgcgaaacg atttgatgtc atagtcaata
     7861 tttctgagct tagaagttaa gaaatattga taggtatagc catacataga gaaaaaacaa
     7921 tatgaaatat gattagtcat tacttttgag ttttttaatt taaggactgg attttccagt
     7981 ccttttttat gatgataaaa aacaagcctc gcagagcaca cgtttttaat gacttatttg
     8041 gtttttaata agtctagtat gttaaaactt ttctgtgttt aggtgttata atcatatgca
     8101 gtggaagttg atttattatg tcttattcaa ttattcgagt tgttaaagtg aaatcaaaaa
     8161 caaatacaag aggtattcaa agacatattc aaagagaaaa taaaaattat gaaaatatag
     8221 atattgattt aagtaagtca tatttaaact atgatttagt caatgatact aagtttgatt
     8281 ttaataaaaa aattgatgag aaaatcgaaa aaaattataa agggaaacgc aaaattagaa
     8341 cagatgccat taaacatatt gatggattaa ttacatcaga taatgtgttt tttaatcaat
     8401 tatctgaaga agagacaaaa tagtttttta agcatgctaa agcgttcttg gaagaagaat
     8461 acggtaaaga aaatctttta tatgcgacag tacatatgga tgagataaca ccacatatgc
     8521 attatggcgt agttcctatt actaaagatg gacgtttaag tgcgaaagaa gttgtaggta
     8581 ataaaaaagc attaaccgaa ttccaagata gattcaatac gtatattaat aagcaaggct
     8641 atgatttaaa acgaggaatt tctagacaac ttacaaaaga aaaacatgat caagttagcc
     8701 gttataaaca aaaaacagaa tatcataaac aaatgcatat gcgctagaag caaatagagg
     8761 attatttaaa gcaagaaaat gataaattga tacaaaacta ccaaaaatca ttagatacgc
     8821 ttaaaaagct tctgagtgtc atgtatgaaa ttaaaactaa aaatgtaggt ggatggtttc
     8881 ataaagagaa gcaagaaaca ggcaatattg taattactaa aactgatttt gaaaaataca
     8941 ctaaacaaat taaagcagca cagatgatat tagatgatta tgaatgtatt aaaagtggga
     9001 agtcattaaa aaaagcagag aaacaaaatg aaagtttagt taatgaattg actagtgtcc
     9061 atatggaaaa tgaaaagctg gttgaagaat ttaatgattt agcacaacgt tataattatt
     9121 tgttgtcaga gaatgaaaag aaagataaag aactaaatta tacgcttaag ttattcaatc
     9181 aagtttttaa aatcattaaa agtatgatga aagaagagcg ttatcataca ctaataaatc
     9241 atattgataa tcatttagac aattctaaaa tacgtgaagt aatgacgata gataacaatg
     9301 atgaacagtt ctttaagaag aaatatcaag cacaagaacg agaaatcatt tttaaagaag
     9361 atagagaaga tggttataca ttataaaaat agatatttaa atgcatttgt ttgagattaa
     9421 ttatttttta ggatgaaaaa taggtagccc ccctcgaact ttttaatata tttttataaa
     9481 tctttttaaa aaccttttta ggggcttgtt cagccctaga gcaacaaggg atttcaatgc
     9541 ctaaaggagt gtttttgcca cataaaagga gtgtttttgc cacataaaag gagtgttttt
     9601 gccacataaa aggagtgttt ttgccacata aaaggagtgt tttaaaaagt tgtactcctt
     9661 ttatggtttg acattgataa taaagtagtt tattgtgaat aaaagttttc aaatgggagt
     9721 gtatattcaa acgatactcc tttaggttcg taataaaggg ggctgtatca tgacaggaga
     9781 gacagttgta tataaaaatg aaatgaattt agttccatta aggcgattta cagctactga
     9841 aattaattta ttttttgcga tgtgtaataa attaaaagag caagatacta atacattacg
     9901 tttgtctttt gatgaattaa aaaaattaag taattatagt ccagaaacac gtaatattaa
     9961 tagatttgct aatgatttag ataatgtgta taaaaagatg ttgaatttaa ccattagata
    10021 tgaagatgat gatgtaatag aaagattcgt tttattcaat cattatagaa tccataagcg
    10081 agaacaatat ctagaaatat caacttcttc aaatttaaaa cacatattga attcaattac
    10141 taataatttt acaaaatttg aattaaaaga aatgactagg cttaaatcta catattctaa
    10201 aaatatgttt agattactta aacaatacaa acatacaggc tatttaaaaa ttcacatcga
    10261 tgattttaaa aatcgtttag atatacctaa atcttatcgt atgactgata ttaacaaaaa
    10321 tgtttttaaa cctattatta ttgaattagg ttctatattt aataatctta ctatcaataa
    10381 aatcaaagcg aaaaaagggc gtaaaattga atggatagag ttcacatttg acgctgagaa
    10441 acgcattcat aacaaacgac aacctcaaat gtccaagata gataaatcaa gacaatatgt
    10501 gaggcgtgaa aagacgccta aatggttaga agaacggtca tatgaaaagc aacctcaaaa
    10561 agattacgac ccacaattag agaaagaacg agaggatttt ttaaaacaac ttgaactgaa
    10621 ttgggaataa cgcaactttt cgttgcgagt tggttaacta aaagtatcgt gacttttcaa
    10681 ttattttaat tatagtttga gttaaggagt gattttatga aattagctga agctataaaa
    10741 gaacagagag aattaaaagg atggtctcaa gaggaattag ccaatatatt aaaggtttcc
    10801 agacaaagtg tttctaaatg ggaaagtgcc aaaaactatc cttccttaga tattttgatt
    10861 gctatgagcg atttattcgg aatctcttta gagcatttaa ttaaaggaga tgcacgtttt
    10921 aaaaaagtta ttttagaggg aaattataga gagagtaaca gggtaccttc catagttgat
    10981 tttctttatg atttttggtg gcttttcttt cctgttgccg gatttttagt ttgggccatt
    11041 cattcgttca tgggttgatt ttaaaatcaa aatttaatgc acatcaggca atatgaaaaa
    11101 ggaaagtatt tttacgacac cttgaaatgt attgataaga atagaagagt ttacaaaagg
    11161 caatatgaaa tatatactaa aagtaacaag aatttcgttt gtaaacgcac caaaccccga
    11221 actattcgcc gtagttcggg gttttttggt gaattggctt taatcgtcta tattattttt
    11281 tatcacgttt atggagctaa taaccaaata acgtaacgag acaaccactg attgccgttg
    11341 tcatgatgtg aacaagaatt tcattcataa atcgcacctc ctctctacgc cgaattgaca
    11401 tctgagagat agacgacctt cacattataa caaatctctc cttccatgaa ataacatgaa
    11461 tattaataaa tgttgttata tattaaagat aaataaatat aagggtggga ttgataatgt
    11521 ttggcgatca aagacaagaa gctacaaaat atgtcattaa agaaggttat caagatattt
    11581 atttcctcaa caaaaacggg gaatggtatt atttcgaagt aagaagtgtc tggcgtggca
    11641 aacatattat tagagttaaa gatggtttgt taggttggag aaaagaaata gtaactgaat
    11701 aaagatacaa aatacttgcc aatgcaaaag aatgaaaacg cgtcaaatca acgatgtata
    11761 aaagatgcat aaaagataat gaaagtaaaa agccataaat gccgtcatat aggcatttat
    11821 ggctttttgt ttggttaata taatcgatga ttatcagaag ttcaattcac ataaaaaagc
    11881 acggctaatt tatgaacttg tcgtaccttt ttaattaaaa ttagttacca aatagacgac
    11941 tccaaaaccc ctttttagct tccgtttctt cactcttttc ttctatatca ctatcattgt
    12001 catttggatt agctgaaacg tcttctcttt gtgcttcttc accttgtgta ctttcgtcgc
    12061 tttgacttcc agatatatcc ttatattgaa tctctgtttc tttagtttct tcctgatctc
    12121 ggttaatatt tacaggacta gtagtatatg ttttttcttg tgcatcaacg ttttgtctag
    12181 cgttagtaga tgtatcaaag ggataattaa ggtgtttttc tttttctaat tgattttcta
    12241 atttttgaat ctttttatta ctctctaatg ccagaacttg ctgattctca agcaattttg
    12301 atctttcttg caactctttt ctgtaaaatt caattgttgt ttcatgacga ctttcttgtt
    12361 tttcaatttg attatttaaa gtattaatct gatttttaag tatttctact atttgagcat
    12421 cgcttttaga ttcaaaatta atattatccg actctttttt ttgtttgtct tcagtagcac
    12481 tttcaaaacc atatttcttt ttattttcat ttactctttg aatgattttt tctatatcgg
    12541 tatcttcttt aataaacgaa gtgttttcct gttttattgt ctctatattc aatctcttaa
    12601 tattattaaa aatcgtttgc ttactaaccc ctaattcttc actcaacctt tttacacttt
    12661 tcatatcatc aaccctttat aaaatgttta taaaccctta gtaaaatatt ataagcttat
    12721 aacgattaat aataaagtaa tcgacaaata aaaatataaa ttcgccaacc tttttatcaa
    12781 gggtttgtaa agggtttatg gtgtttatca aaggtttata aacctatatt ttcccttttt
    12841 taagtttatt caaagtgcta taataacctt aatataacaa aaaccccaac tactggtaat
    12901 agtcggggtt tggtaataaa acgtttagaa actgtttatt tatataattt tcgatactta
    12961 gattatataa tgaataatat cttagtgcaa gcacctagat tattaaataa acacaaaaaa
    13021 tgtaagcgtt ttattacctc ttaccaaact aaaattcgga aagaggtttt tattatgtca
    13081 attaaacttt acaacataaa tgaggaatac agagaaaagt tttatcaaat accgaaagta
    13141 ttttttacaa atcctaaata tatcaagtta tccaatgatg caaaaatggc atggggtatt
    13201 cttagagatc gtttagattt atcaattaaa aatggttggg ttgattctaa aacaggtaat
    13261 atttattttt attacaaaaa tgaaaaccca caaaatatat taaattgtaa aaaagataaa
    13321 gtaattaaaa taaagaaaga acttaattga agccgaatta ctactacaag aaagacaagg
    13381 tttaaataaa acaaataaac tttatttagt taaaccagaa gtaacagaag atgatattta
    13441 taaaattcaa aagcaagaaa acactgttga gagcagcaat ggcaaggaag tcggaaaaac
    13501 cgacttcaag aagtcgataa aacagaatct aatgatactg aacttagcta tactgaatct
    13561 aacaatactg aaataaatga tatgaatgac ttgaatgata tagagtccga aaatgaaatt
    13621 agtaatcatt caagtcaatg caaggttatt taatgcaaac aattttaact gaatttgaag
    13681 aaacacatgc actttatatg cgccgtaaaa acatgaaaca atataatatc tttaatcaat
    13741 cattcaaatg attttataac aatcaaagat acatcaaaat atttcgttaa atcatctgac
    13801 tttttaactg atagcgatga aaaaatcaag aaattgtcag tgatctagaa aaagatttgt
    13861 atcgaaaacg tatgttaagt tatggtggat tgcttaaata aaaacataaa attttaaact
    13921 tagacgatgc tgaagatggc aatttaatta atacaagtga tgaagataaa atagcaaatg
    13981 aagaagaaaa ggcacattcg attacggcaa ttttgaaaag caaaattatt atttaaggca
    14041 ttagtgataa ctgatgtctt tttttattac ttttttgtaa aaaaagtgct gtcttatttt
    14101 tgtgacaaat gctgtatgta gtgtcacaaa agtgtgacac ttgagctttt tatgacccca
    14161 cacaaaaaca ttttcgaagc cttgaaatat ctggctttgc cagatccccc attcattgat
    14221 ggggacaaat ttcccttatg ctcttacgga gattttagag ttaaattaaa atttctaagt
    14281 ttttgataag aaaagccctg caggaattta gaagaacgtg gggaagtttt aaagaatttt
    14341 atgctaattt ttaatttgca tgtaaatggg cagtgtctaa aaaatagtta ttgtttttgc
    14401 tcaaattttt gtttttgaaa atagcatata tttatttggc tcatatttgc gttttaaaag
    14461 cgtatataag ttttagataa aactatatga atcaccccta aatctttaaa atgccctctt
    14521 aaaattcaaa ataagggcat aaaaaataaa agccccccca gataaagtga ggtagctttt
    14581 tctttcgacg ccgtatcgtc gatgtaattc aaaaaagtta tgggctataa atctacatca
    14641 ctttttcaag aatgtagtag caatattcaa cgcaaattaa tttttataac tgctcggaat
    14701 atctcaagcc gtttctttaa ttttgaaata aaaaaatcga cgaaggtcga ttacgttttt
    14761 gcacatgtcg atttatccga cgtataagtg cgcccttacg ggatttaact agattatgac
    14821 gacgaatttt agacctgtaa agcaataaca attaggtcta ccaggtagat aaagtgtaca
    14881 aagtagaacc gaaaaagtta tttattttga agatttttcg tagatataat ttgctaataa
    14941 acttcctaaa actgaataaa gaaaaatagt taaactcatg taatcactcc ataattctga
    15001 agtgtctcca tgtctacgca tagacacgca tttat
//
LOCUS       DAMZLE010000058        14875 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMZLE010000058 DAMZLE010000000
VERSION     DAMZLE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.058"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(33..1091)
                     /gene="hisC"
                     /locus_tag="POB92_001529"
     CDS             complement(33..1091)
                     /gene="hisC"
                     /locus_tag="POB92_001529"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDI6579032.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTEEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(1331..2845)
                     /locus_tag="POB92_001530"
     CDS             complement(1331..2845)
                     /locus_tag="POB92_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDI6579033.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(2838..3815)
                     /locus_tag="POB92_001531"
     CDS             complement(2838..3815)
                     /locus_tag="POB92_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579034.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDVAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4037..5818)
                     /gene="recQ"
                     /locus_tag="POB92_001532"
     CDS             complement(4037..5818)
                     /gene="recQ"
                     /locus_tag="POB92_001532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDI6579035.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(5830..7713)
                     /locus_tag="POB92_001533"
     CDS             complement(5830..7713)
                     /locus_tag="POB92_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI6579036.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVSLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(7984..9924)
                     /gene="ltaS"
                     /locus_tag="POB92_001534"
     CDS             complement(7984..9924)
                     /gene="ltaS"
                     /locus_tag="POB92_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDI6579037.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(10398..11402)
                     /locus_tag="POB92_001535"
     CDS             complement(10398..11402)
                     /locus_tag="POB92_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDI6579038.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(11386..12096)
                     /locus_tag="POB92_001536"
     CDS             complement(11386..12096)
                     /locus_tag="POB92_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDI6579039.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(12184..12390)
                     /locus_tag="POB92_001537"
     CDS             complement(12184..12390)
                     /locus_tag="POB92_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579040.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(12456..13064)
                     /locus_tag="POB92_001538"
     CDS             complement(12456..13064)
                     /locus_tag="POB92_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDI6579041.1"
                     /translation="MQLFETMKIENGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESEFETIVSPLPEKSSFTAKFQVLPKVVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(13064..14215)
                     /locus_tag="POB92_001539"
     CDS             complement(13064..14215)
                     /locus_tag="POB92_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDI6579042.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLGKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(14199..14792)
                     /locus_tag="POB92_001540"
     CDS             complement(14199..14792)
                     /locus_tag="POB92_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDI6579043.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT         4622 a         2683 c         2040 g         5530 t
ORIGIN      
        1 agaacattga tttatgtccc agcctgcact tactattcgt atttaaagtt tgataaaact
       61 tctaacattt tatcattttg ttctttaaaa ccaattgtaa ttctaacacc agttggaaat
      121 ggtcgcgtaa tacaccctac atttaaaagt gcttcataaa gttcatttac ccgctttgtt
      181 tttacaaata taaaattcgt ttgacttggc aagaaatact cactttgagg taattgataa
      241 aatctttcgc gttcaacact atttttatgt gttacctctt ttaaatattg ttgatcttca
      301 agtgctgcaa ctgctgcgta ttcagatata cgtgtcacat taaatggtgg tctaatgatg
      361 ttccattttt caatcgcatg ttcacttgct accacatatc ctacacgtaa acccgctaat
      421 ccatacgcct ttgaaaatgt acgtaataag aaagcattgt catatttttg ttgcaaagca
      481 agtgtatccg ggtagtcctc ttctgtcaca aattcaaaat aagcttcatc tataattact
      541 ggtacatgtg gaggtacttg agataaaaac gaatctaagc tctcatgatt aaaatatgta
      601 cctgtaggat tatttggatt acataaccat accaatgacg tatcttcatt aacttctttt
      661 aaaatacctt ctaaatcgaa gccaccatct tttaaaggta cttgtatcac attagctgat
      721 tcaacaatcg cattgtgata atattgaccg aatgtcgctt cacttgtaac aatagtatcc
      781 cctggcgtta atacagctct agaaatcatt aatataactt catctaatcc cgcaccaaat
      841 aaaatgcgtg attgatctac atttaaatgt ttactaatcg ccgcttttaa tgtcggtgat
      901 cctgtttcag gataataata taactcatct aagtgtgctg atatcgcttc tttaacttta
      961 ggcgatggtc catacaaatt ttcatttgat gcaagtttat ataaatctcc ttcaatgcca
     1021 tacttttctt tcaatgccct tggagataaa ccaggctgat atgctgatag ttgattaagt
     1081 tgttctttca taataaaaac aacctcctta gaattaactg aaattttaaa aaacttatat
     1141 tcattatatc gctctatgtt tctaaaatgc aatgctacta ttttcacatc ataatagctt
     1201 acattgtgtt taacatgtca tattttttaa tcattattgc atgtttaaat tgaacataat
     1261 tatagattta aatataacaa cgccacataa gatataattt ggtgttacgc atcttatgtg
     1321 gcgttggtcg ttatttgatt aacccttttg cttttaaata atctttcgca actgtatatg
     1381 ggtcttcatt tttaactgtt actttatagt tcatcatttg catatcttca tcagatattt
     1441 tgttttctag tttgtttaac ggtttcttaa tttctggatg tttctttaaa aagctttctt
     1501 taaataatgg tgctccttga tatggtggaa atacgtgctt atcatctttt aacacaacca
     1561 tatcatattg ttttaattca gcgtcagttg aatatgcatc tattaaatta atattacctt
     1621 tattgatcgc ttgatatctc aacttaggtt ccattgtacg tatgttatct aaatttaaat
     1681 tataagcctt ttgaacagct ttgtaaccat ctggacgatc attaaattcc aatgtaaatc
     1741 ctggtttaag ttgatcttta accttattta aatcaccaat tgtacgtata ttatgttgtt
     1801 tagcaaaatc acgttttaca gctaaagcat acgtattgtt atacttcatt ggttttaaca
     1861 tagtcatttg atatttcttt tcaagacttt gcttagcttg ttcataaact tttttctctt
     1921 cttttgactt caatggttct tttgttaatt cgcctaaaac tgttccagta aattctaaat
     1981 acccatctat atcgtcagat tttaaagcat taaataaaaa tgctgttttg cccataccat
     2041 ctttcacttc tacagtattt ttggtctctt cttctattaa aattttatac atatttgtaa
     2101 taatcgatgg ctcggagcca agctttccag ctaacgtaat tttatcacct ttttgtgcaa
     2161 acataggaat agcgatagcc agtataataa tcatcactat aacacctaac gtcatcaata
     2221 actttttata agataattta gccataaatc ttaaaattaa atcaaatata attgccaata
     2281 aggctgctgg aattgcacct aataatatca acgatgcatt gttacggtct atacctaata
     2341 aaattaaatc tcctagtccg cctgcaccaa ttaatgctgc tagtgttgct gtacctataa
     2401 ttaataccat agccgttctt acaccagcca ttataacagg cattgctatc ggaagttcga
     2461 ctttagttaa acgtctaaat ggtttcatac ctataccttt agccgcttca atgagtgatg
     2521 gatcaacttc tttaattcca gtatacgtat tccttaaaat tggtaacaac gcatacacta
     2581 caagtgcaat aattgctggc acacgaccga taccaaataa aggaatcatt aaacctaata
     2641 atgccaacga tggtatggtt tgaagaattg ccgcaatatt cattacgatt tcagatatcg
     2701 ttttagtctt cgttaataaa atacctaatg gtaccgcaat agcagttgca atcaataatg
     2761 cgataaatga tatttgaata tgttctatca ttgtcgaaaa gagttgcccc ttacgttcac
     2821 tcaatatgtc gaaaaagtta gtcatgttga gctacctcct ttttctggga caaatatttg
     2881 aagatatctt tcctatcaat aacatattga cctacgctat cttcttgcat gacaatgaca
     2941 cgctcgctct ctgataaaag ttgatacaat acttcaattg gttgattgtc ataaacaatt
     3001 ggataagcgc tcatagatgt aacctcatcg attggtttca taatatccaa gtcacggata
     3061 attgcgttct cttcaacaca tggcgcaaca tcttctaaat gactacccat aaattgttta
     3121 acaaattcac tttgaggatt atttttaaat ccttctggtg tgtcaatttg ttcaatatgc
     3181 ccttcattca aaagacaaat cttatcacca agtttcatcg cctcttgaat atcatgtgta
     3241 acaaatatga ttgtcttctt aattttagtt tgtaattcaa ttaaatcatc ttgaagtttt
     3301 tctcggctga ttgggtctaa tgcactaaac ggttcatcca ttaaaataac tggtggatca
     3361 gctgctaacg cacgtataac tcctacacgt tgtcgttgcc cccctgacaa ttcatcaggt
     3421 tttctgtttt tatatttttc aggttctaat ccaaccattt caagtaattc atctactctt
     3481 ttatctatat ctttttcttt ccactttttc atttgtggca cttgtgcaat attttctttg
     3541 attgtcatat gtgggaataa tgcaatctgc tgcaatacgt atccaatatc ccaacgcatt
     3601 tcgtatactg gataatcact tattggttta tctttaaaat aaatataacc ttcacttaag
     3661 tgaatgagtc gattaatcat ttttaatgtc gtagtttttc cacaacctga aggtccaatt
     3721 agcacaaaaa attcaccctc attaatattg aaactaatgt tatcgacagc aacatgtttg
     3781 ccataacgct tagttacatt tttaaactta atcactttgc cacctctttt tttctcatag
     3841 cataaaaccg agattatatg tatgtattcc ctatttaacc acgtttatta caattttcaa
     3901 atttaaatga tttatccttg aactttttta ataaaataat gaataatagg taatctccag
     3961 ttaagaaata gtgttatttt accttgaatt caaaaaaaca cccagtaaaa caaggaatgc
     4021 ttactaggtg tcttcactat actttggctt tataattttg aatcgtttct aaaaatgctg
     4081 gacaataatg ttttaatttg taactaccta cgccatcaat attaatcata tcttgtttcg
     4141 aagcaggctt acgtttagca aattcctcca acgtgtaatc agaaaatata cttacaggtg
     4201 ctatcgttaa tttgtcactt aactttttac gaacttctac caactgactg aataatactc
     4261 ggtcaacccc ttcaaccgta tttataaata ctttttcagt cgctttttgc ttaaatggtg
     4321 ttgtgaatac ttctacttca ttactgagta attttttaat tgaagtatca cacattaata
     4381 tttcatcatt ttcatttaag aaccctttga atcttaattc atctattaag tgacttaatt
     4441 ctgatgttgt gtaacctttc attaaaccat gggttgaaat ttgatcataa cctttgtact
     4501 taatataatc tgttgactct cctcttagca cttgaatgat aacactataa ctctcttgtt
     4561 gtttcatacg agcgatgcaa ctaataatca tcttagcttc ttgtgtcata ttatatgatt
     4621 tatcttgttg aacacaatta ctacattgtt cacattcttc taatttttca ttcggttcaa
     4681 aataatggac aattgttgct tctaaacatt tttttgtttt tgtatattga atcattttag
     4741 ttaacttttc gcccatttta tctttatagt catcatcagc ttgagagact gttataaaat
     4801 actcgtgtaa attgatatcg cgttcgctaa ataacaaaat acattcactt ttcaacccgt
     4861 cacgacctgc acgaccggct tcttgataat aagattctaa atctccaggc atattataat
     4921 gaataacaaa acgtacattg gatttatcaa tacccatacc aaaagcattt gtagcaacga
     4981 ctactttaac acgatcaaat aagaaatcat tctgcgcttc ttctctttct ttattgctca
     5041 aacctgcatg atatataaca ctttcaattt tctggctttc taaggcttct tgaagctctt
     5101 caacttgctt acgtgtagaa caataaataa tacctgcatc ttcatcatgt gttttaatat
     5161 aatccaatat aaatttttga cgttgataag taggatttac tttaaaaatt aagtttctac
     5221 gcttagtact cgttttaatt tgatcagttt gagcgatatt taacttttct ctaatatctt
     5281 gctgtacttc aaccgtggca gttgctgtca acgctattat tgtaaaatct tgaggtaacg
     5341 taaatacttt tgaaataaca ttttggtaac tcggcctgaa atcatgaccc catttagaaa
     5401 tacaatgcgc ttcatcaaac gcgactaagt gaatctttat acgctgaagc atatttaaaa
     5461 aatatcggtt ttcaaatcgt tctggtgcaa catacaaaaa ttgaatttct ccatttgata
     5521 atgctttttc aatacgttgt tgctcttttt gagtcaaact actatttaaa aaagcagctt
     5581 gaattcccat cgcttttaat tgatccactt gatctttcat taatgatatt agtggactta
     5641 ttacaattgt tgtaccacct aacaataaac ctggtacttg atagcatata gacttacctc
     5701 caccagttgg taagacacca agcacattac gatggtctaa tactttgcta ataatttctt
     5761 cttgtcctgg tcgaaacgtt tcatacccaa agtaatgcga taatgtttgt tgcatcataa
     5821 attgacccct tattgttgtt ctttaatttc ttctaactca ctccatcttg tgatgtctaa
     5881 atcatattga atttcaagtt gttctttttc ttcgtttaat tctttaattt tcccataatc
     5941 tgcacttgcc tcaatcatga gcacatctat ttcttccatt cttacttccg cttgttctat
     6001 gcgtttcatc aattgttcat attctaactt ttctttatat gataaaccat ttttcttacg
     6061 tacagttgta gaagatttag attgttgctt caatgtggat ttatttttat ctaatgattt
     6121 tttataactt tcataatctt caaaagttcc gataatcttt tccatctgac catcatgaat
     6181 aaaccaatat gactgtgcaa ctttatttaa aaagtagcga tcatggctta cggtaatcac
     6241 tgtaccaccg aaagtatgaa tataatcttc aagtattgtt aaagtctcag tatctaaatc
     6301 atttgtcggt tcatctaaca acagaacatt tggctggtgt acgagtagac gtaataaata
     6361 caaacgcttt tgctctccac cagataattt ataaactttt ttaccatgag ttgcacttgg
     6421 aaataaaaat cgttcaagaa gttgtgtaat agataccgag gttccatctt tttcttttgc
     6481 gatctcactt tcttctctta aataatcaat catacgaata tctctatcca gggtctcatc
     6541 tgtttgctta aaataagcta ctttaaccgt ctgtccagtc ttcaatttac cttcgaattg
     6601 ttggtcttct ccactcaaaa tatttaagag tgttgtttta ccagctccat ttggcccaac
     6661 aacaccaata cgctcacctt tttgaataat ttccgtcaga tgttcaaata atactttatc
     6721 attaatagcc tttgataagt cttctaattc gaacacttgc ttacctaatc ttgaataagc
     6781 aagattcaat tcacctttat cgtctttata ttgctggtta acttcatttt ctaggtcatt
     6841 aaatcgatta attctagctt gttgctttgt agtacgagcc ttagctccag ccctcatcca
     6901 agcaagttct tccttatata aagctcgttg cttttgttgt tgcttttgaa gtgatacttc
     6961 tctttcagcg cgcatttcaa tataagattc atagttacca ggatatgacg ctaacttacc
     7021 tctgtttagt tcaataattc tagtggaaac ttcatttaaa aaatatcgat catgggttac
     7081 gaataaaaca gtatgaggat attgcttcac ataattgatc aaccagctga ttgattcgaa
     7141 gtctaaatgg ttcgtaggtt catctaataa caataaatct ggttgttcta ttaatgtttt
     7201 tgcaagtaca acacgttttt gttgtccgcc ggataattct ttaatgtatt tagtagtatc
     7261 atgtatacct agttttgaga ggattgtttt aatttcagcg ttatagtccc aagcatcatg
     7321 ttgatccata gcatcttgcg catccatcat tcgcttgaac aatttatcac tttggtcatc
     7381 cgcataagct tgtactgcct gctcatattg cttgatgatg cgtaaagttg ttgtatcaga
     7441 acttaatact gcatcaaaaa ctgtcatatc ttcattaagg tcctgtttct gagacgaata
     7501 tcgaattcga tattgatttg gatgcataac attggctgta aaatcatcat caataccacc
     7561 aattactttt aacaacgtac ttttccctgt accatttatg cctactaaac ctattttttc
     7621 accttctgaa attgataaat ctaggttatc gaatatagtc ttatcggcat aagatttatt
     7681 taaatgttca attttatatg cttccatacc catcttcctt cttctgttga aatctctacg
     7741 tacttaaatc agttctatac attatacacc tttttgaaat caaaagaaat cgataccgtt
     7801 aagtgtcata atttaatata ttgttgcttc aactataaat aaaacttgtt aaggctttga
     7861 tgactatgct aatgatcttt ataatatacc tttttatcaa cacatataat gtaaaacata
     7921 atataaaaga acataataaa aacccggatt gatattttat caatccgagt tcgtgtttaa
     7981 atattatttt ttagagtttg ctttaggtcc tgtttcatat ttatacttcg aaggatttac
     8041 ctttttgaag tctggatttt tgtagaatct aaacaaatca ccattaagca cgttgtcact
     8101 catttcgaga tccttttcaa cttgcttttt attcttttcg aaatcagctg gttgagtagt
     8161 tatgagttca ttatttttat tagaataaat cttaccatta acatatttat aatcttttgt
     8221 tataaagtca ccatttctga atggaactac ttgattatga cctttagaga ataaatcagt
     8281 accgaacatt aaataattct tcgtatctat accagccaaa tgtaaaattg ttggcattac
     8341 atcgacttga ccagcatatt cattattgat accaccagat ttaccaggga ttttaatcca
     8401 gaaaccagtt ctgtttaaat ctgtaaattt agctggtgtg attttttcac ctaatagttt
     8461 ttccatggca ttgttatggt tttcagagat accatagtgg tcaccataaa tcataatcac
     8521 tgaattgtca tataatcctt ttttcttcaa gtcattaata tattcttcta atgcttcgtc
     8581 taaataacgc gctgtttgaa tataaccatc aactgttgca tcacctgtgt ttgacttctc
     8641 aatagtcgca tccttttcat ctaatgtgaa tggatagtgg ttagtcaaag taattaaatg
     8701 agaatagaat ggtgatttca tcttagcttg ataattagca gaatctttaa agaaaatttt
     8761 gtctttcaag cctaagttta caacgttttt atcagacatg tcatagtatg ttgcatcata
     8821 gaatttatcg ataccaaagt gtttatatac ttggtctctg ttccagaatg ttttatagtc
     8881 accgtgcatg acatcagatt tgtagccttg cttttgatct aaaattgctg gtagtgactg
     8941 atacgtatta tctcctttta atgaaaaggc agaaccttgc ggtaaaccgt ataaactgtt
     9001 atccattgta aattcagagt cagatgtttt accttgacct gtttgatgga aaaagttagg
     9061 gaagtatgtg aattgctctt tcccacttga taatttgttt aaaaacggtg ttacttcttt
     9121 accattaacc tttttattaa ttaagaaggt ttggaaactt tctaaatgaa tcttaataat
     9181 atttttcttc tttgccaccc cataatattc tgggttaggc tctgtttgac gttgtttcgt
     9241 ataatttaat acttttgtta agtcatcttc agatgctaac gctttttgtt gattattttc
     9301 gatagtttta acaccatcgt atactgtaaa gttatacggt cctaaatatt tcactaaata
     9361 tttatggtca aatgtacgtg ttaataattc tggtctgtca gtttcagcaa aagctaagtt
     9421 taagaagaat aatgctactg aagccgccat tacgacagga acaaatttct tactaaatgc
     9481 ttttgtgtct aaccattttg ttttaaatat taaaatgaat aagtaaacta acgtatcaat
     9541 gaaataaaca aagtcatacc atttgaatga tgcactaacc gcaccaccca tagattctac
     9601 gttacctact tggtttaaag tactaaacgt taaaaaatcc gagaagaatc taaagtacac
     9661 aacattggca tataatagga acgtcaataa gaagccgcct atgaacatga accaaaatgc
     9721 ttttttgcct ttaaagaata ggaacacact taaaaccagt gctactaaac tataaggatt
     9781 catcaataat attaagtttt gtactaaacc tttaacacct aaagaaaaat caacataata
     9841 agaaaaatac gtcttcaagg taatcgttat tacggttaat aagaagaacg caaaaagact
     9901 aattttcttt ttttgtgaac tcatgattct ttcccccgtt atttagataa taaatcttgc
     9961 gaaagtactt gaataaatta gatattaaca ttatgtagtt aattaataag tgcgtttaaa
    10021 agttaacatt gtcataatta tagtaattac tttcagattc aagtttataa ttttcattat
    10081 ttgtagcttt caactttgat ttaacaagta ttttgggcta caactcatat ttgcttgtgt
    10141 aagtttcttt atttaatatt atcattcaat taacaaattt aaaatttgta atcaaattgt
    10201 aataataagc atagctcaat taataaatat agagtaattt tagtcaaaat tcaagtaaaa
    10261 acacacttca tcttttcatt ttactgtgaa acgaattaga atatgataca tttcgcagat
    10321 aaaacacagt attatttcat tatacattag gaggaaatta tttgcatcgg actcgagtat
    10381 gagctacgta ccaaatatta tttcatcatt tctaattgca ttctatattc tattccatgg
    10441 aataattggt ttaaccagtt actatactta acaaggttct tttcagccgt taaaatatct
    10501 ataaattgaa atttcaaacg tgatccttgc ggtttttgtg ctaatttcgt taaatgataa
    10561 cttgcgattg taccgatttg cgggtagcta cctagcgtat aatgatcatt taataagata
    10621 ataggtgtgc catcacgctt aatttgtatc gttccttttt tgacagtctg atacggtggc
    10681 atatcttcat aaaaggcttt gattttttca ccttcaagca tcatccccat gcgatttgct
    10741 ttgctcgtta ctttataatc atgtttcaca aatctttgaa tggcatcttc tttaaaatct
    10801 tccgtacctt tatttttaac gacatgaaat acatcagaca tataattaaa tgacaaggcg
    10861 tatccatcaa taccccaatc tgtttgtttc gtgtgagcaa ggttttcaaa taacttatga
    10921 tgacgagctg tataatctct cttaagcttt atttcatcgc catcttgtaa tgttctacct
    10981 ttaaaaccac caatttttac attaaagtcg gttgagttag atcctaacca tgcatctaat
    11041 tcaaatccgc ctcccacagc taaatacact cgcgatgtat aacttgtttc tctaaacttt
    11101 aaaacatcgc ctttatctaa caaatacaat ttatatggag atatagtcat atgctcagtg
    11161 taagctttga cattaccccc tgctaatgca atcagcgtag gttctgtaaa acgaatcgtt
    11221 gccattttat tagtcatttc caaagttgct tcattcttgt catttgcaac taatcgatta
    11281 gcaatttcat gtgctaaagt atcaagtgca ccacatggaa ttacaccatc atgttcatat
    11341 ccccttctgc caaagtcctg aaagctactg aataagccac ttttttcaat tatgattgac
    11401 atctttaaac gctcctactt ctaattcatt ttcattgata ggtctaaatt taatattatc
    11461 gcctaacttt aagcgtgcga aatctgattc tttcggatta aaaagtgata atggtgtata
    11521 accaataact aaccaatcat taattgtatc cgtagttaca ataccgcatt ttttcccttc
    11581 aagtactaca gaaccagctg gaatgaattt tttctgttta ctcgtgtgat taatatacaa
    11641 tttcttattt aatccagtta aataaggaaa cccaggtgaa tatcccatca tcgaaacaaa
    11701 atattgcgcc ttagaatgta attcaataaa agtttctagc ttgattttgt aatgttttaa
    11761 aagtgattct aaatccggac catatttcga accatacaca attggtattt caatcaaatc
    11821 ttgctggtta aaatcatgtt ttatgtttaa atgaatcgat tctattagtg ctttcatgta
    11881 taaaaaaggt gattgtatat tatggtgttt aatcatatcc cttgcgtcat agacaatcat
    11941 catgtctgat tctgatggca caatttctat aataaatgga taattttgtt caattaaata
    12001 tgagcgaagt gttaataaat cttcggttaa acttttagat acttcttttt caattgcgac
    12061 tacaatggct tggtcacctt gactatatat tttcatttgt acacctcttc aatatttggt
    12121 tactttactt tcatttttaa ttatagaatc gattgaatag ttcattttac ctactcacta
    12181 aatttactgg tgatacatta aatccattgc ccgcacaaca aaaggcatga gttcgtgaat
    12241 taatgcgtat acaaggataa agcttataac agtagtaatt gttgctatca aacgaacaac
    12301 atatattcta ttttcagata gcaatcgatt cactaatcga taatttacgt atacaagaat
    12361 taacaacagc acaatataaa caataatcat atttggcccc atttaatttt taaatttgtt
    12421 ttcacatcat tttctacatt attatcataa taattttaaa ggcaaaggtg gacatcggca
    12481 acctctcgta aactatttat caaaaataaa cgtatctcat tgttatgata tttataaatc
    12541 aattcgtttt tattaaagtc tttttcaaca agtttgtcac tatctattaa ataatcacgc
    12601 atgcatcctt ttaagaaatc atctttataa cttggtgtgt accattttcc atcctcttca
    12661 atgacaatgt tgccaatatc aaattcaagg accttgccgt cctctgaagt tagcaatatt
    12721 aaatctgttt catgattgtg agctaaatgc tttcgttctg tcgttttatt atttataaaa
    12781 gttggattaa ctactttggg caacacttga aattttgctg taaaactact tttctcaggt
    12841 aaaggtgaca ctatcgtttc aaattcgctt tcagcattta atacgatttt aagtctatat
    12901 tggccactgt gatactttgt tgttacatcg tttaattcat ttcgccatgc atgttcatca
    12961 aatttaaagt ttaatcgctc agaagaacat tttatgcgat tagtatgata agtaagtcta
    13021 gggatatgtc cattttcaat tttcattgtt tcaaataatt gcattataac atctccaaaa
    13081 tcttggtttt tgcgtaaaat tcattcactt catcttttgg cttagaatca attgtaatac
    13141 ctgctccgac tccataaatc gcttgtccat atttatactc aatagtacga atcggaatat
    13201 taaaaatcat tttatcatct tcagttggaa gtaatagtcc aattgctccg cagtatatac
    13261 cacgaggtga actttctaat tgtttaatat atttcattgt attcagtttc ggtgcacctg
    13321 taatcgaacc acaaggaaat aacgatgtta agatttgagt taggtctgta ttagttttta
    13381 aagtaccact taccatcgaa gtcatttgaa atacagtttt atatgcctca ataaaaaata
    13441 gtttatacac cttaattgag ccactctgtg atattctccc tatatcgttt cttagtaaat
    13501 caacaatcat gacattttct gcacgatctt tcgaagaagt ttgcaatgtt ttataatact
    13561 gttgatcttc agcttccgtt ttacctctag gcattgtccc tttcatcggt ttgcttatga
    13621 taacgttatc gacattgtta aactgtcctt tttgaaaaaa taattctggt gagatagacg
    13681 ctacttggat ttcatctgtt tgtaataacg cagtataatt accattacta aattgagtta
    13741 atcgttcata taaagtacta ataggataat aaatgttatc cgttaagcgc gccgtatagt
    13801 tcacttgata cgtttcgcct tcaacaattg cttgttggac acgtttaata ttagtcatca
    13861 taacctcaga agattcaaca aatgaaaaat gatgctttga tacataagaa gtttgatgtt
    13921 catatgttga atttatgctt tccgcttttt caaaactata agctactgca taaatatcat
    13981 ctttacctaa tgaatgtgta cacataacat gattaaaata ctttgccgct tcgtaactta
    14041 aatataacga gacatatcta ccttgtcgtt gctgcgcttg tgcaaagtgt atcacttctc
    14101 ccacatcagc caacttagta gcaacatact tctttataaa tccctttaat tgaatatggt
    14161 attgcttata ttcattttca gttaaatagt agcgataatt atattctatt ctcacagtaa
    14221 tcacctacct tcgataaaaa taattcaact tgtcgataac cgtattcact caaaatagat
    14281 tcaggatgat attgcacacc aaaaaccgga aatgtaatat gctcaaatgc cataataatc
    14341 gcttcatcgt ttttggctgt aatctttaag caatttggaa aagtcgctcc gtcagcaatt
    14401 aatgaatgat aacgcattac attgaaattt tgaggcagtc cttgaaaaat accttcattg
    14461 gtatggcgta actgtgtagt atgtccgtgt acaggatgat agccgtgaat gatatttcca
    14521 ccaaaataag acacgataca ttgaaatcct aagcatacac ctagtatagg tacacgctga
    14581 taaaattgtt ctaacacttc attcaatata ggataatcat ccggattacc cggcccaggt
    14641 gaaataacaa ttgcttttgg cttcatatta atgacgtctt ctatcagcag attatcaata
    14701 ccaacaactt gaactgttag tttcgtttga gtcttaatat agtctattaa attatatgta
    14761 aatgaatcat tattatctat gactagaatc attgtatact ccgttctaaa tgtgttttat
    14821 ttttataata tgtattggat gtagctaaaa ctttaaaagc attgtcatta tcctg
//
LOCUS       DAMZLE010000059        14515 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMZLE010000059 DAMZLE010000000
VERSION     DAMZLE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.059"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(210..569)
                     /locus_tag="POB92_001541"
     CDS             complement(210..569)
                     /locus_tag="POB92_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDI6579044.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGILLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(588..1433)
                     /locus_tag="POB92_001542"
     CDS             complement(588..1433)
                     /locus_tag="POB92_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6579045.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNPESMVEVFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            1905..2585
                     /locus_tag="POB92_001543"
     CDS             1905..2585
                     /locus_tag="POB92_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDI6579046.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGITKTNRGVYYDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDFKLRKQLISQSGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGRQI
                     QKILVEMK"
     gene            2871..3566
                     /locus_tag="POB92_001544"
     CDS             2871..3566
                     /locus_tag="POB92_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDI6579047.1"
                     /translation="MKMKSIVKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            3857..4927
                     /locus_tag="POB92_001545"
     CDS             3857..4927
                     /locus_tag="POB92_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDI6579048.1"
                     /translation="MKMRTITKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTQQTKMTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFLLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIDGTNIDNIEVNIK"
     gene            5046..5183
                     /locus_tag="POB92_001546"
     CDS             5046..5183
                     /locus_tag="POB92_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579049.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            5292..6137
                     /locus_tag="POB92_001547"
     CDS             5292..6137
                     /locus_tag="POB92_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDI6579050.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPPSTKVETPQQV
                     ANATTPSSTKVEAPQQAANATTPPSSNVDTSPPQSPTTKQVPTEINPKFKDLRAYYTK
                     PSLEFKNEIGIILKKWTTIRFMNVVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLE
                     ENNYNLEKYSVGGITKSNSKKVDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKEL
                     DFKLRKQLIEKNNLYGNVGSGKIVIKMKNGGKYTFELHKKLQENRMADVIDGTNIDNI
                     EVNIK"
     gene            6501..7205
                     /locus_tag="POB92_001548"
     CDS             6501..7205
                     /locus_tag="POB92_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDI6579051.1"
                     /translation="MKMAAIAKASLALGILATGTITSLHQTVNASEHEAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKHQKFTRIQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            7651..8349
                     /locus_tag="POB92_001549"
     CDS             7651..8349
                     /locus_tag="POB92_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HDI6579052.1"
                     /translation="MKLKALAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELINVDGLYRQHLTDKGAYVWKNLKDYYIGLLGEDSKKFKSDVYGDLDAF
                     LVIEEEPVKGRQYSIGGISKTNSKEFKEREVDVKVTRKADRDTTSTKDSKFKITKEEI
                     SLKELDFKLRQKLMKEENLYDAINHRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTID
                     GTKIKEINVELEYK"
     gene            8771..9466
                     /locus_tag="POB92_001550"
     CDS             8771..9466
                     /locus_tag="POB92_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDI6579053.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLSGKDKDKYKEGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNQSSETNTPLFIKKVYGGNLDASIESFLINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITFNLKDGEKQEIDLGDKLQFEHMGDVLNS
                     KDIQNIAVTINQI"
     gene            9805..10503
                     /locus_tag="POB92_001551"
     CDS             9805..10503
                     /locus_tag="POB92_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDI6579054.1"
                     /translation="MKFTAIAKAIFVLGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSYDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGAADKGRIVINMKDENKYEIDLSDKLGFERMADV
                     INSEQIKNIEVNLK"
     gene            10882..11580
                     /locus_tag="POB92_001552"
     CDS             10882..11580
                     /locus_tag="POB92_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDI6579055.1"
                     /translation="MKFTALAKATLALGILTTGVFTTESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKIDPKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            11939..12622
                     /locus_tag="POB92_001553"
     CDS             11939..12622
                     /locus_tag="POB92_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDI6579056.1"
                     /translation="MKLTAIAKAALALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            12884..14440
                     /locus_tag="POB92_001554"
     CDS             12884..14440
                     /locus_tag="POB92_001554"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6579057.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALAGEDITYQETWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQEDYVLFIDASNDFEKGKNQNHLTDAQVERIINTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEVEQEINAY
                     LKELGVLKDE"
BASE COUNT         5696 a         2022 c         2549 g         4248 t
ORIGIN      
        1 aattaaatca gatatgggtt ttcgtcatta aataattttt ctataaggaa acagaggcaa
       61 cgttactgtc acctctgctt gaaataatta ggatgaaata ctctgagtct aaagtccaca
      121 cccaattcgc tcacatcctc caaacctcca aacctccaaa cctccaaacc tccaaacctc
      181 caaacctcca aacccccaaa caccaatatt taccaaagca ttgatatgag tgccaatact
      241 ggcaatgtac cttgtttgaa aaagatacta atattgcttg aaaggccacc ataaatagca
      301 acgccaatga tatacactaa aatagctgcg catatttctt ttggattact gctgataaac
      361 aaaccgtata ttagcaaaat tccgattaaa ccgttatata cgccttggtt cttcaaaagt
      421 aggttaatat ttttgtcttt caatttatcg acgcttatat taaatgtctc gctagtcttt
      481 ttggaagttg tagcaatcgt ttcaaggtac ataatataga aaaactctaa tgccacaaat
      541 atgattaaaa ttgttgagat gatattcact gtaacgctcc tttattatta aatattttct
      601 tgtaaaaatg attgcagtgt ctgtggttga tcattgacta attgttggaa atcattggat
      661 tcttggtcta atagtcctct tgctcctgcg tcgtacattg atgccaataa tgcaccaaag
      721 cctttaggtt catcgtacat ttctgcaaat gtctctaatg aaacgggctc atatttaatt
      781 tctgtgcctg atgcctcaga taaaattgca gcaagttctt tcatatcata actgtagcct
      841 gataataaat agcgtttgcc ccaagtatct ggatttttaa taatagcaat gacacctcta
      901 gcaatatcat ttctagtaat ataattaata cgaccatcac cagctggata aatcagttta
      961 tgcatattca tcaattctgg taaatatggt ttaagtggat ccatgtacat tgccattctt
     1021 acataggtat agtcaatacc acttgttgcc aatagacgtg ctgcataacc aaaataagga
     1081 ctcatatgga atggattatt atgctgatct gcgtaataac ctatgaaaat gatatgagca
     1141 acgccacttt gctttgccgc atatactaaa ttttccactt ctggaatacg tttgaatgat
     1201 ggatggataa tacttggaat aaacacaacg gtatccattc ctttaaatac ctctaccatg
     1261 ctttcgggat taaaataatc taattgtcga acaggaactt ttccgcgcca atcttctgga
     1321 actttctcaa catttctaac accaatgtga aaatgatcta tgtgatttgc aatggcttga
     1381 tttgtaatat gtgtgcctaa atgacctgta gcacctgtta acataatatt cattcacttc
     1441 atctcctaat ctttatatac ataacataat acttattcga ttgttttcaa aacatttgat
     1501 tttataaaaa attgtaatct gtatttatta tcgctgtgta tagtaaatac gtatatgtta
     1561 ttaatgttga aaataccgta atgacgcgtt ttagttgatg tgtatcacta atatctttgg
     1621 aaattttaat caggtactac gacaatatga tgtctgtttt gtgtctgaaa gttttacagt
     1681 ttttaaaata aaaatggtat aaagtgtgat ttgtataaaa aagagtctcg acggataaga
     1741 attgattaat aacagttagc attttattaa ttaccttaac aatgattcaa gtttagttaa
     1801 atgaggttta atttgaaagg ggatagcgcc tcaatataat gtaggtagat tgttcatatt
     1861 acgtaattga aaaatcaaat ttaaatagat tggggctaaa aattatgaaa tttaaagcga
     1921 tagcaaaagc aagtttagca ttgggaatgt tagcaacagg tgtaattaca tcgaatgtac
     1981 aatcagtaca agcgaaaaca gaagttaaac aacaaagtga ggctgattta aaactttatt
     2041 ataatggacc aagttttgaa tataaaaaag taactggata tggatttatt gaaggtaaag
     2101 atagatttat tgattttata tacaatggac aatataataa aatatcttta gttggttctg
     2161 ataaagataa atataatgaa gaagttaacc cagatataga tgtgtttgtc gttagagaag
     2221 gaaacggtag acaagctgat aatcattcga ttggtggcat aacaaaaact aatagaggag
     2281 tgtattatga ctatatacac acaccaatcc ttgaaatcaa gaaaggtaaa gaagaaccac
     2341 aaagtagtct ataccaaatt tataaagaag acatctcact aaaagaactt gattttaaat
     2401 taagaaagca attaattagt caaagtggct tgtattcaaa tggtcttaaa caaggtcaaa
     2461 ttacaattac aatgaatgat ggcacaacac atacaatcga tttaagtcaa aaacttgaaa
     2521 aagaacgtat gggcgagtct atcgatggca gacaaataca aaaaattcta gtagaaatga
     2581 aataatactt tctaacaaca aagcgctatg ttgaatagtg cttgttatgg aaatatatgg
     2641 aagttaagcg acgtactgtt gcttagcttc tttttttgag gggaaaagtt acaaaactca
     2701 cacaaacagt cgcaccacgc attatctttt gcttaaatag cttaatcata ttttatgaat
     2761 agttaaaaac aggttaatgt gaatatctga atacagctcc tataatatgg gtgtatggtt
     2821 caaattacgt aataaaacaa tctaattata atagattgga gcatacagct atgaaaatga
     2881 aatcaattgt aaaaataagt ttgttattag gaatattagc aacaggtgta aacactacaa
     2941 cggaaaaacc agttcatgcc gaaaagaaac ctattgtaat aagtgaaaat agcaaaaaat
     3001 taaaagctta ttatactcaa cctagtattg aatataaaaa tgtgacaggt tatatcagtt
     3061 tcattcaacc aagtattaaa tttatgaata tcatagatgg taattctgtt aataatattg
     3121 ctttaattgg caaagataag caacattatc atacgggtgt acatcgtaat cttaatatat
     3181 tttacgttaa tgaggataag agatttgaag gtgcaaagta ctccattggc ggtatcacga
     3241 gtgcaaacga taaagctgtc gacctaatag cagaagcaag agttattaaa gcagatcata
     3301 ttggtgaata tgattatgac tttttcccat ttaaaataga taaagaagca atgtcattga
     3361 aagagattga ttttaaatta agaaaatacc ttattgataa ttatggtctt tacggtgaaa
     3421 tgagtacagg gaaaattacc gtcaaaaaga aatactacgg aaagtataca tttgaattgg
     3481 ataaaaagtt acaagaagac cggatgtccg atgttatcaa tgtcacagat attgatagaa
     3541 ttgaaatcaa agttagaaaa gcataacaca catacttgac gacgaaataa tttgaaattg
     3601 aaatagagag gttaagtgac gatcaaacgt tgcttaactt ctttttaatg cttaaaaatc
     3661 atttcaaagg cacatagaaa cgctatatta acctcataat cactcattat tttttgctta
     3721 aattacttaa taatacttca ataattgtta aaaggggttt aatgtgatta tcttagaatg
     3781 ccatctataa tgatgttgta tgattcaaat tacgtaaaaa gacaatcgaa tataatatag
     3841 attggagcat acaattatga aaatgagaac aattactaaa accagtttag cactagggct
     3901 tttaacaaca ggcgcaatta cagtaacgac gcaatcggtc aaagcagaaa aaatacaatc
     3961 aactaaagtt gacaaagtac caacgcttaa agcagagcga ttagcaatga taaacataac
     4021 agcaggtgca aattcagcga caacacaagc agctaacaca agacaagaac gcacgcctaa
     4081 actcgaaaag gcaccaaata ctaatgagga aaaaacctca gcttccaaaa tagaaaaaat
     4141 atcacaacct aaacaagaag agcagaaaac gcttaatata tcagcaacgc cagcgcctaa
     4201 acaagaacaa tcacaaacga caaccgaatc tacaacgcag caaactaaaa tgacaacacc
     4261 tccatcaaca aacacgccac aaccaatgca atctactaaa tcagacacac cacaatctcc
     4321 aaccataaaa caagcacaaa cagatatgac tcctaaatat gaagatttaa gagcgtatta
     4381 cacgaaaccg agttttgaat ttgaaaagca gtttggattt ttgctcaaac catggacgac
     4441 ggttaggttt atgaatgtta ttccaaatag gttcatctat aaaatagctt tagttggaaa
     4501 agatgagaaa aaatataaag atggacctta cgataatatc gatgtattta tcgttttaga
     4561 agacaataaa tatcaattga aaaaatattc tgtcggtggc atcacgaaga ctaatagtaa
     4621 aaaagttaat cacaaagtag aattaagcat tactaaaaaa gataatcaag gtatgatttc
     4681 acgcgatgtt tcagaataca tgattactaa ggaagagatt tccttgaaag agcttgattt
     4741 taaattgaga aaacaactta ttgaaaaaca taatctttac ggtaacatgg gttcaggaac
     4801 aatcgttatt aaaatgaaaa acggtgggaa atatacgttt gaattacaca aaaaactgca
     4861 agagcatcgt atggcagacg tcatagatgg cactaatatt gataacattg aagtgaatat
     4921 aaaataatca tgacattctc taaatagaag ctgtcatcgg aaaaacaaga agttaagtga
     4981 caacggttta catgttgctt agcttctttt attatgcgta atgatgtaaa aagacgaata
     5041 ttcatttgtt tgtaaaagtg gcatttctat gtcttaaaag tgacgaaact tcaaatgtgc
     5101 caagtgttga atcacatcaa aatcattttt atttaacgaa cattatggat ttcttaattt
     5161 acttaacgat gattcaaata tagttaaaca aggtttaatg tgaatggagc aatgcgccat
     5221 ctataataaa gctgtatgat tcaatgaatg taatcgaaca aatctaataa ttacgaatgg
     5281 agcatacaac tatgaaaata acaacaattg ctaaaacaag tttagcacta ggccttttaa
     5341 caacaggtgt aatcacaacg acaacgcaag cagcaaatgc gacaacaccg ccttcaacta
     5401 aagtggaaac accgcaacaa gtagcaaatg caacaacacc atcttcaact aaagtggaag
     5461 caccgcaaca agcagcaaac gcgacaacac caccttcgtc taatgtagac acatcaccac
     5521 cacaatcgcc aaccacaaaa caagtaccaa cagaaataaa tcctaaattt aaagatttaa
     5581 gagcgtatta tacgaaacca agtttagaat ttaaaaatga gattggtatt attttaaaaa
     5641 aatggacgac aataagattt atgaatgttg tcccagatta tttcatatat aaaattgctt
     5701 tagttggtaa agatgataaa aaatatggtg aaggagtaca taggaatgtc gatgtatttg
     5761 tcgttttaga agaaaataat tacaatctcg aaaaatattc tgtcggtggt atcacaaaga
     5821 gtaatagtaa aaaagttgat cacaaagcag gagtaagaat tactaaggaa gataataaag
     5881 gtacaatctc tcatgatgtt tcagaattca agattactaa agaacagatt tccttgaaag
     5941 aacttgattt taaattgaga aaacaactta ttgaaaaaaa taatctgtac ggtaacgttg
     6001 gttcaggtaa aattgttatt aaaatgaaaa acggtggaaa gtacacgttt gaattgcaca
     6061 aaaaattaca agaaaatcgc atggcagatg tcatagatgg cactaatatt gataacattg
     6121 aagtgaatat aaaataatca tgacattctc taaatagaag ctgtcatcgg aaaaacaaga
     6181 agttaagtga caacggccta catgttgctt agcttctttt gttatgttcg atgatttgag
     6241 aacccgaatt ttcgatgggt ccaaatatga cgtggaagag tcctgaattt atctgtaaat
     6301 ccctatctat cgggtgtgga gcacaacgga atcagtttta tttaacgaac attatagatt
     6361 ccttaattta cttaataatg attcaatgat tattaaacat ggtttaatgt gaaaggtcaa
     6421 atacgccaat tataataaag ctgtatgatt caatagacgt aagcgaacaa atctaataat
     6481 tacgaatgga gcatacaact atgaaaatgg cagcaattgc gaaagcaagt ttagcattag
     6541 gtattttagc aacaggaaca ataacgtcat tgcatcaaac tgtaaatgcg agtgaacatg
     6601 aagcaaaata tgaaaatgtg acaaaagata tctttgactt aagagattac tatagtggcg
     6661 caagtaagga acttaaaaat gttactggtt atcgttatag caaaggtggc aagcattacc
     6721 ttatctttga taaacatcaa aagttcacta gaatacaaat ttttggtaaa gatatagaaa
     6781 gatttaaagc acgcaaaaat ccgggattag acatatttgt tgttaaagaa gcggaaaacc
     6841 gtaatggcac agtgttttca tatggtggtg tcactaagaa aaatcaagac gcttattatg
     6901 attatataaa cgcaccaaga tttcaaatca agagagatga aggtgacggt attgctacgt
     6961 acggtagagt acactacatt tataaagaag agatttcact taaagaactc gactttaaat
     7021 tgagacagta tttaattcaa aattttgatc tgtataaaaa gtttcctaaa gatagtaaga
     7081 taaaagtgat aatgaaagat ggcggctatt atacgtttga acttaataaa aaattacaaa
     7141 caaatcgcat gagtgacgtc attgacggta gaaatattga aaaaatagaa gccaacatta
     7201 gataatgcaa tgaaatatgg ataatagtaa aatatggata gtatagagga gttaggcaac
     7261 ataagttgct tagcttcttt tttgtgttgg agagatgaaa aagaagcgta tcgatgaata
     7321 ataaaaacac caataaaact tgtgggaata gttgatacct atagtcgcgc gttgtccttt
     7381 tcgtgacatg aaacaatgtg gaaaacataa ttaaattgag ggaaagtgtg aatagttaaa
     7441 aaagctgcgt taagtttaaa aaatagatta acgctgttag gattctatta attagcttaa
     7501 cattggttca aaaatagtta aaaagaggtt aattcatagc ttagtattcc gcttatataa
     7561 tgatagtaga ttgttcgtat tacgtaattg aaataatcat ataaaaatat attaagacaa
     7621 aatttataaa tagattggga gaatagtact atgaaattaa aagcgttagc taaagcaaca
     7681 ttagtattgg gattgttagc tactggtgta ataacaacag aaagtcaaac agtaaaagcg
     7741 gcagaatcaa ctcaaggtca acacaattat aaatcattaa aatactacta tagcaagcca
     7801 agtatagagt taataaatgt agatggtctg tatagacaac atttaactga taaaggtgca
     7861 tatgtatgga aaaatcttaa agattattat attgggctac taggtgaaga tagtaagaaa
     7921 ttcaaatcag atgtatacgg ggacctagat gcatttttag tcatagaaga agaacctgtt
     7981 aaaggaagac aatattcaat tggcggtata agtaagacaa atagtaaaga atttaaagaa
     8041 agagaagtcg atgttaaagt aacaagaaaa gcagacagag atactacatc aactaaagat
     8101 agtaaattta aaattacaaa agaagaaatc tcgttaaaag agttagattt taaattaaga
     8161 caaaaattga tgaaagaaga gaatttatac gatgcaatta accatagaaa aggtaaaatt
     8221 gtagttaaaa tggaagatga taagttttat actttcgaac ttacaaaaaa attacaaccg
     8281 catcgcatgg gtgacacgat agatggtacc aaaatcaaag aaattaatgt tgagctagaa
     8341 tataaataat ctttggacaa gcagactagt aattgtaggg aagttaagcg ataacatatt
     8401 gcttagcttc ttttttgttt tgttatgatg aaaaaaggag cgggtttatg atcaagtttt
     8461 tggaaaaacg gttgatactt atagtcgcgc gttgtccttt tcgtgacatg aaacaatgtg
     8521 gaaaacataa ttaaattgag ggaaagtgtg aatagttaaa aaattagtat tgtgttataa
     8581 aaaataatta atactgttag gatttcatta actaacttaa cgttggttca aaaatagtta
     8641 aaaagaggtt aattcatagc gcagtatctc acttatataa tgatagtaga ttgttcgtat
     8701 tacgtaattg aattaatcat ataaaaatat attaagacaa aatttataaa tagattggga
     8761 gaatagtact gtgaaattaa aaacgttagc taaagcaaca ttggcattag gcttattaac
     8821 tactggtgtg attacatcag aaggccaagc agtgcaagca aaagaaaagc aagagagagt
     8881 acaacattta tatgatatta aagacttaca tcgatactac tcatcagaaa gttttgaatt
     8941 cagtaatatt agtggtaagg ttgaaaatta taacggttct aacgttgtac gctttaacca
     9001 agaaaatcaa aatcaccaat tattcttatc aggaaaagat aaagataaat ataaagaagg
     9061 ccttgaaggc cagaatgtct ttgtggtaaa agaattaatt gatccaaacg gtagactatc
     9121 tactgttggt ggtgtaacga agaaaaataa ccaatcttct gaaactaata cacctttatt
     9181 tataaaaaaa gtgtatggcg gaaatttaga tgcatcaatt gaatcatttt taattaataa
     9241 agaagaagtt tcactgaaag aacttgattt caaaattaga caacatttag ttaaaaatta
     9301 tggtttatat aaaggtacga ctaaatacgg taagatcact ttcaatttga aagatggaga
     9361 aaagcaagaa attgatttag gtgataaatt gcaattcgag cacatgggcg atgtgttgaa
     9421 tagtaaggat attcaaaata tagcagtgac tattaatcaa atttaaagta agtaatcaat
     9481 gactctaaag taataaattt gaagcagctt agcgatgaaa tgttgaatag atacgtacac
     9541 cttacataaa ggagcgtatt taaaacaacc ttgtcgttag gcttttttta cgttttataa
     9601 cgcagggtat gagcgtacta aaaattcaca ttacttctga aagtgatgtc cattgaatat
     9661 taattagttc ttcattaacc atgatttaat tttaattaaa cgagtgttaa tgtcagtctg
     9721 tctcaatgcc ctttataata aatgtgtatt attcaaatta cgtaataaaa gcaatccaat
     9781 atattaagat tggagcatat gaatatgaaa tttacagcga tagctaaagc gatatttgta
     9841 ttaggaatat taacaacaag tgtaatgata acagaaaatc aatcggttaa tgcaaaagga
     9901 aagtatgaaa aaatgaaccg tttatatgat acaaacaagt tacatcaata ctattcagga
     9961 cctagttatg agttaacaaa tgttagtggc caaagtcaag gttattatga ctctaacgtt
    10021 ttgcttttta accaacaaaa tcaaaagttc caagtgtttt tattgggaaa agatgaaaat
    10081 aaatacaaag aaaaaacaca tggtttagat gtctttgcgg taccggaatt agtagattta
    10141 gatggaagaa tatttagtgt tagtggtgta acaaagaaaa atgtaaaatc aatatttgag
    10201 tctctaagaa cgccgaactt actagttaaa aaaatagacg ataaagacgg tttttcgtat
    10261 gatgaatttt tctttattca aaaggaagaa gtatcattga aggaacttga tttcaaaata
    10321 agaaaactgt taattaaaaa atacaaattg tatgaagggg cagctgataa aggtagaatt
    10381 gttattaata tgaaagatga aaataagtat gaaattgatt taagtgataa attaggtttc
    10441 gagcgtatgg cagatgtcat taatagtgaa caaattaaaa acatcgaagt gaatttgaaa
    10501 taatcaatga tatatataga atgaaagctt aagaagcggt ttaataatcc catgtttaat
    10561 gattttgata cgtgttttaa taataaaaac atatcgaaca ttgactacgt tattaagctg
    10621 cttttttgta cactttgtat cgaataactt aagatctaaa actaatcgga aagaacaatg
    10681 attcccctaa aaaaatttat gttgctatta aaaatcagtt aatacgaatg ttaacatacg
    10741 tttgattttc attaataatg attcaagttt atttaaatga gcgttaatgt cagtctgttt
    10801 tgatgcacct tataataaag acagatagtt caaattacgt aataataaca atccaatata
    10861 tcaagattgg agcaaataaa tatgaaattt acggcattag caaaagcaac attagcatta
    10921 ggaatattaa ctacaggtgt gtttacaaca gaaagtaaag ctgttcacgc gaaagtagaa
    10981 cttgatgaga cacaacgcaa atattatatc aatatgctac atcaatacta ttctgaagaa
    11041 agttttgaac caacaaatat tagtgttaaa agcgaagatt actatggctc taacgtttta
    11101 aactttaaac aacgaaataa agcttttaaa gtatttttac ttggtgacga taaaaataaa
    11161 tataaagaaa aaacacatgg ccttgatgtc tttgcagtac ctgaattaat agatataaaa
    11221 ggtggcatat atagcgttgg cggtataaca aagaaaaatg tgagatcagt gtttggattt
    11281 gtaagtaatc caagtctaca agttaaaaaa atcgatccta aacatggctt ttcgataaat
    11341 gagttgttct ttattcaaaa ggaagaagta tcgttgaagg aactggattt taaaataaga
    11401 aaaatgttag tcgaaaaata tagattgtat aaaggcgcgt cagataaagg tagaatcgtt
    11461 attaatatga aagacgaaaa gaaatatgta attgatttaa gtgaaaaatt aagttttgat
    11521 cgtatgtttg atgtaatgga tagtaagcaa attaaaaata ttgaagtgaa tttgaattaa
    11581 ttaagtataa taacttaaga agcgacttaa cgacaaaatg tgaattgaca tgcatgtcct
    11641 taaataagga actgtgttaa atacattact gttgttaagt tgttttttgc gtttcaaaga
    11701 gcagaacaga gtaacatcat cagttgtagt aaacgataat ctagtaaaac aactaaatga
    11761 aataatgaaa ttcatttaac ctgaacatta aaatatattt gtttttcatt aagaataatt
    11821 caagtatatt taaatcgagg ttaattatcg tatgaaacga tgcacgttat aataaaaatg
    11881 tatgattcaa attacgtaat gaaaacaatc caatatatta agattggagc aaaacaatat
    11941 gaaattaaca gcgatagcta aagctgcatt agctttagga attttaacaa caggaacttt
    12001 aacaacagaa gttcattcag gtcatgcaaa acaaaatcaa aagtcagtaa ataaacatga
    12061 caaggaagca ttataccgat actacactgg aaagactatg gaaatgaaaa atattagtgc
    12121 tttgaaacat ggtaaaaata acttgcgttt taagtttaga ggtattaaga ttcaagtttt
    12181 actgcctgga aatgataaaa gtaaatttca acagcgtagt tatgaggggt tagatgtgtt
    12241 ttttgttcaa gaaaaaagag ataagcacga tatattttat actgttggtg gtgtaataca
    12301 gaataataaa acatctggag ttgtcagtgc accaatatta aatatttcaa aagaaaaggg
    12361 tgaagatgct tttgtgaaag gttaccctta ttacattaaa aaagaaaaaa taacactaaa
    12421 agagctggat tataagttga gaaagcatct aatcgaaaaa tatggacttt ataaaacaat
    12481 ctcaaaagat ggtagggtca aaattagctt gaaagatggc agtttttata accttgattt
    12541 aagatctaaa ttaaaattca aatatatggg ggaagtcata gaaagcaaac aaattaaaga
    12601 tattgaagtt aacttaaagt aaataattac gaataataaa aagtaattga agcggcttaa
    12661 cgatgaaaag taaattgatg cgcatacctt accaaaagga tgcatcaatc gatatcgtcg
    12721 ttaagctgtt ttggtttacg tttcatggat tctaccccaa ttttcataaa tataaaaatt
    12781 ccaccaccaa catcaaaatt ctcaacatcg caacataccc aaatgttata ataaatctat
    12841 tacacaaaga gataaattac ttattcaaag gcggaggaat cacatgtcta ttactgaaaa
    12901 acaacgtcag caacaagctg aattacataa aaaattatgg tcgattgcga atgatttaag
    12961 agggaacatg gatgcgagtg aattccgtaa ttacatttta ggcttgattt tctatcgctt
    13021 cttatccgaa aaagcagaac aagaatatgc agatgcgttg gcaggtgaag atatcacgta
    13081 tcaagagaca tgggcagatg aagaatatcg tgaagactta aaagctgaat taattgatca
    13141 agtcggttac ttcattgaac cacaagattt attcagtgcg atgattcgtg aaattgaaac
    13201 gcaagatttc gatatcgaac atctggcgac ggcaattcgt aaagttgaaa catcaacatt
    13261 aggtgaagaa agtgaaaatg actttatcgg actgttcagc gatatggact taagttcaac
    13321 gcgactaggt aacaatgtca aagaacgtac tgctttaatc tctaaagtca tggttaatct
    13381 tgacgattta ccatttgttc acagtgatat ggaaattgat atgttaggtg atgcatatga
    13441 attcctaatt gggcgctttg cggcgacagc aggtaaaaaa gcaggcgagt tctatacacc
    13501 acaacaagta tctaagatac tggcgaagat tgtcacagac ggtaaagata aattacgtca
    13561 tgtgtacgac ccaacatgtg gttccggttc attattgtta cgtgttggta aagaaacgca
    13621 agtgtatcgt tatttcggtc aagaacgtaa caataccact tacaacttag cacgcatgaa
    13681 catgttatta catgatgtac gttatgaaaa tttcgatatc cgtaatgatg acacgttgga
    13741 aaatccagcc tttttaggac atacatttga tgcggttatt gcgaacccac catacagtgc
    13801 gaaatggaca gcagattcaa aatttgaaaa tgacgaacga ttcagcggat acggcaaact
    13861 tgcgccaaag tccaaagcag actttgcctt tattcaacac atggtacatt acttagacga
    13921 tgaaggtacc atggccgttg tactcccaca tggtgtctta ttccgtggtg ctgcagaagg
    13981 tgtcattcgt cgttatttaa ttgaagaaaa gaactactta gaagccgtga ttggcttacc
    14041 agccaatatt ttctatggga caagtattcc aacatgtatt ttagtattta aaaaatgtcg
    14101 ccaacaagaa gactatgtat tatttatcga tgcatccaat gattttgaaa aaggaaaaaa
    14161 tcaaaaccat ttaaccgatg cccaagtcga acgcattatt aacacatata agcgtaagga
    14221 aacaattgat aaatatagct acagtgcgac attacaagag atcgccgata acgattacaa
    14281 cttaaacata ccgagatatg tcgatacatt cgaagaagaa gcaccgattg atttagatca
    14341 agtccaacaa gatttgaaaa atatcgacaa agaaatcgca gaagttgaac aagaaatcaa
    14401 tgcatacctg aaagaacttg gggtgttgaa agatgagtaa tacacaaaag aaaaatgtgc
    14461 cagagttgag gttcccaggg tttgaaggcg aatgggaaga gaagaagtta gggga
//
LOCUS       DAMZLE010000060        14395 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMZLE010000060 DAMZLE010000000
VERSION     DAMZLE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.060"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            78..371
                     /gene="esxA"
                     /locus_tag="POB92_001555"
     CDS             78..371
                     /gene="esxA"
                     /locus_tag="POB92_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDI6579058.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            454..3483
                     /gene="esaA"
                     /locus_tag="POB92_001556"
     CDS             454..3483
                     /gene="esaA"
                     /locus_tag="POB92_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDI6579059.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSRSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKTTSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3483..3941
                     /gene="essA"
                     /locus_tag="POB92_001557"
     CDS             3483..3941
                     /gene="essA"
                     /locus_tag="POB92_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDI6579060.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3913..4155
                     /gene="esaB"
                     /locus_tag="POB92_001558"
     CDS             3913..4155
                     /gene="esaB"
                     /locus_tag="POB92_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDI6579061.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4168..5502
                     /gene="essB"
                     /locus_tag="POB92_001559"
     CDS             4168..5502
                     /gene="essB"
                     /locus_tag="POB92_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDI6579062.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5524..9949
                     /gene="essC"
                     /locus_tag="POB92_001560"
                     /pseudo
     CDS             5524..9949
                     /gene="essC"
                     /locus_tag="POB92_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            9979..10371
                     /gene="esaC"
                     /locus_tag="POB92_001561"
     CDS             9979..10371
                     /gene="esaC"
                     /locus_tag="POB92_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDI6579063.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKTSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10387..10701
                     /gene="esxB"
                     /locus_tag="POB92_001562"
     CDS             10387..10701
                     /gene="esxB"
                     /locus_tag="POB92_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDI6579064.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPIADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10701..11375
                     /locus_tag="POB92_001563"
     CDS             10701..11375
                     /locus_tag="POB92_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDI6579065.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFNTDENH
                     INKAEE"
     gene            11375..11692
                     /locus_tag="POB92_001564"
     CDS             11375..11692
                     /locus_tag="POB92_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579066.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKASHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11702..13546
                     /locus_tag="POB92_001565"
     CDS             11702..13546
                     /locus_tag="POB92_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDI6579067.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRN
                     NHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRSFKENGQWYKLEKL
                     WEKAIKSGKSIENVKVEVKYKGNSQRPTEFSVRYNINGEQFLKNIINK"
     gene            13557..14051
                     /locus_tag="POB92_001566"
     CDS             13557..14051
                     /locus_tag="POB92_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6579068.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYAMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQKLRNIFKKEGHEPWTSCEF
                     DFTNEGKLKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKQIEQYIKE
                     QEEE"
     gene            14262..>14395
                     /locus_tag="POB92_001567"
     CDS             14262..>14395
                     /locus_tag="POB92_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDI6579069.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     "
BASE COUNT         5640 a         1970 c         2712 g         4073 t
ORIGIN      
        1 agtaaggagc actttttcaa aaaatagtgt ccctaaaaag ttttgataaa cttaaaatat
       61 tcaggaggtt tctagttatg gcaatgatta agatgagtcc agaggaaatc agagcaaaat
      121 cgcaatctta cgggcaaggt tcagaccaaa tccgtcaaat tttatctgat ttaacacgtg
      181 cacaaggtga aattgcagcg aactgggaag gtcaagcttt cagccgtttc gaagagcaat
      241 tccaacaact tagtcctaaa gtagaaaaat ttgcacaatt attagaagaa attaaacaac
      301 aattgaatag cactgctgat gccgttcaag aacaagacca acaactttct aataatttcg
      361 gtttgcaata agcattctga aattggcaaa gtcacatttt ctaatgtggc tttgcttatc
      421 attttttaag aaaacaactg aaaggaaata agcatgaaaa agaaaaattg gatttatgca
      481 ttaattgtca ctttaattat tataattgcc atagttagta tgatattttt tgttcaaaca
      541 aaatatggag atcaatcaga aaaaggatcc cgaagtgtaa gtaataaaaa taataaaata
      601 catatcgcaa ttgttaacga ggatcaacca acgacatata acggtaaaaa agttgagctg
      661 ggtcaagcat ttattaaaag gttagcaaat gagaaaaact ataaatttga aacagtaaca
      721 cgaaacgttg ctgagtctgg tttgaaaaat ggtggatacc aagtcatgat tgttatccca
      781 gaaaactttt caaaattggc aatgcaatta gacgctaaaa caccatcgaa aatatcgcta
      841 cagtataaaa cagctgtagg acaaaaagaa gaagtagcta aaaacacaga aaaagttgta
      901 agtaatgtac ttaacgactt taacaaaaac ttagtcgaaa tttatttaac aagtatcatt
      961 gataatttac ataatgcaca aaaaaatgtt ggcgctatta tgacgcgtga acatggtgtg
     1021 aatagtaaat tctcgaatta cttattaaat ccaattaacg acttcccgga attatttaca
     1081 gatacgcttg taaattcaat ttctgcaaac aaagatatta caaaatggtt ccaaacatac
     1141 aataaatcat tattgagtgc gaattcagat acgttcagag tgaacacaga ttataatgtt
     1201 tcgactttaa ttgaaaaaca aaattcatta tttgacgagc acaatacagc gatggataaa
     1261 atgttacaag attataaatc gcaaaaagat agcgtggaac ttgataacta tatcaatgca
     1321 ttaaaacaga tggacagcca aattgatcaa caatcaagta tgcaagatac aggtaaagaa
     1381 gaatataaac aaactgttaa agaaaactta gataaattaa gagaaatcat tcaatcacaa
     1441 gagtcaccat tttcaaaagg tatgattgaa gactatcgta agcaattaac agaatcgctg
     1501 caagatgagc ttgcaaataa caaagactta caagatgcgc taaatagcat taaaatgaac
     1561 aatgctcaat tcgctgaaaa cttagagaaa caacttcatg atgatattgt caaagaacct
     1621 gatacagata caacatttat ctataacatg tctaaacaag actttatagc tgcaggttta
     1681 aatgaggatg aagctaataa atacgaagca attgtcaaag aagcaaaacg ttataaaaac
     1741 gaatataatt tgaaaaaacc gttagcagaa cacattaatt taacagatta cgataaccaa
     1801 gttgcgcaag acacaagtag tttgattaat gatggtgtga aagtgcaacg tactgaaacg
     1861 attaaaagta atgatattaa tcaattaact gttgcaacag atcctcattt taattttgaa
     1921 ggcgacatta aaattaatgg taaaaaatat gacattaagg atcaaagtgt tcaactcgat
     1981 acatctaaca aggaatataa agttgaagtc aatggcgttg ctaaattgaa aaaggatgct
     2041 gagaaagatt tcttaaaaga taaaacaatg catttacaat tgttatttgg acaagcaaat
     2101 cgtcaagatg aaccaaatga taagaaaaca acgagtgttg tggatgtaac attgaatcat
     2161 aaccttgatg gtcgcttatc gaaagatgca ttaagccagc aattgagtgc attatctagg
     2221 tttgatgcgc attataaaat gtacacagat acaaaaggca gagaagataa accattcgac
     2281 aacaaacgtt taattgatat gatggttgac caagttatca atgacatgga aagtttcaaa
     2341 gacgataaag tagctgtgtt acatcaaatt gattcaatgg aagaaaactc agacaaactg
     2401 attgatgaca ttttaaataa caaaaagaat acaacaaaaa ataaagaaga tatttctaag
     2461 ctgattgatc agttagaaaa cgttaaaaag acttttgctg aagagccaca agaaccaaaa
     2521 attgataaag gcaaaaatga tgaatttaat acgatgtctt caaatttaga taaagaaatt
     2581 agtagaattt ctgagaaaag tacgcaattg ctatcagata cacaagaatc aaaaacaatt
     2641 gcagattcag ttagtggaca attaaatcaa ttagataata atgtgaataa actacatgcg
     2701 acaggtcgag cattaggcgt aagagcgaat gatttgaacc gtcaaatggc taaaaacgat
     2761 aaagataatg agttattcgc taaagagttt aaaaaagtat tacaaaattc taaagatggc
     2821 gacagacaaa accaagcatt aaaagcattt atgagtaatc cggttcaaaa gaaaaactta
     2881 gaaaatgttt tagctaataa tggtaataca gacgtgattt caccgacatt attcgtatta
     2941 ttgatgtatt tactatcaat gattacagca tatattttct atagttatga acgtgccaaa
     3001 ggacaaatga atttcattaa agatgattat agtagtaaaa accatctttg gaataatgtc
     3061 attacgtcag gtgttattgg tacaactggt ttggtagaag gattaattgt cggtttaatt
     3121 gcaatgaata agttccatgt attagctggc tatagagcga aattcatctt aatggtgatt
     3181 ttaactatga tggtcttcgt acttattaat acgtatttac taagacaggt aaaatctatc
     3241 ggtatgttct taatgattgc tgcattgggt ctatactttg tagctatgaa taatttgaaa
     3301 gcggctggac aaggtgtgac taataaaatt tcaccattgt cttatatcga taacatgttc
     3361 ttcaattatt taaatgcaga gcatcctata ggcttggcgc tagtaatatt aacagtactt
     3421 gtgattattg gctttgtact gaacatgttt ataaaacact ttaagaaaga gagattaatc
     3481 taatgttgat gaatagcgtg attgctttaa cttttttaac agcatctagc aataatggcg
     3541 gacttaatat tgacgtgcaa caagaagagg aaaagcgaat caataatgat ttaaatcaat
     3601 atgatacaac gctatttaat aaagacagca aagcggttaa tgatgcgatt gctaagcaga
     3661 aaaaagaacg acaacaacaa ataaaaaatg atatgtttca aaatcaagcg agtcactcga
     3721 ctcgcttgaa tgaaactaaa aaagtgttat tttccaaatc taacttagaa aagacttcgg
     3781 agagtgataa aagcccctat attcaaaaca agcaggagaa aaaaatattc ccgtacattt
     3841 tgatgtctgt aggggctttt ttgactttag gatttgtcat tttttcaatt cataaaggga
     3901 gacgaacgaa aaatgaatca gcacgtaaaa gtaacatttg attttactaa ttataattac
     3961 ggcacatatg acttagcagt accagcatat ttaccgataa aaaacttaat agctttagta
     4021 ttggatagtt tggacatttc aatatttgat gtcaatacac aaattaaagt gatgacgaaa
     4081 ggtcaattac ttgttgaaaa tgatcgactc attgattatc aaatcgctga tggagatatt
     4141 ttgaagttac tataggagga aaaatagatg gttaaaaatc ataaccctaa aaatgaaatg
     4201 caagatatgt taacgccttt agatgctgaa gaagcagcta aaacaaaatt acgcttagat
     4261 atgagagaga ttcctaagtc ttcaattaaa ccagaacatt ttcatttaat gtacttatta
     4321 gaacaacatt ctccatattt tatagatgct gaattaactg aactacgtga cagtttccaa
     4381 atacattatg acattaatga caatcataca ccttttgata atattaaatc atttactaaa
     4441 aatgaaaaat tacgttactt actcaatatc aaaaatttag aagaagtaaa tcgtacacgc
     4501 tacacatttg tgttggcacc agatgaatta tttttcacaa gagatggatt acccattgct
     4561 aaaacaagag ggttacaaaa tgttgttgat ccattacctg tgtcagaagc tgaattttta
     4621 acaagatata aagcactggt tatctgtgca ttcaatgaga aacaatcatt tgatgcttta
     4681 gttgaaggaa acttagaact acataaagga acgccatttg aaactaaagt tattgaagcg
     4741 gcaacgttag atttactaac ggcattttta gatgaacagt atcagaaaca agaacaagat
     4801 tatagtcaaa attatgcata tgtacgcaaa gtaggacata ccgttttcaa atgggttgct
     4861 atcggtatga caacgttaag tgttttatta attgcattct tagccttttt atatttttca
     4921 gtaatgaagc ataatgagcg cattgaaaaa ggataccaag catttgtaaa ggatgattat
     4981 acgcaagtac taaatacgta tgatgattta gatggtaaaa aattagataa agaggcactt
     5041 tacatttatg ccaaaagtta tatccaaaca aataaacaag gtttagaaaa agataagaaa
     5101 gaaaatttac ttaataatgt gacaccaaat tcaaacaaag actacttatt atattggatg
     5161 gaattaggac aaggacatct tgatgaagcg attaatattg ccacttattt agatgataac
     5221 gatattacaa agttagcgtt gattaataaa ttaaatgaga ttaaaaataa cggagattta
     5281 tcgaatgata aacgttctga agaaacgaaa aagtataacg ataaattgca agatatttta
     5341 gacaaagaaa aacaagttaa agatgaaaaa gcgaaatctg aagaagagaa agcaaaagcg
     5401 aaagatgaga aattaaagca acaagaagag aacgaaaaga aacaaaaaga acaagcacaa
     5461 aaagataaag aaaaacgcca agaagctgaa agaaaaaaat agtataggac tgaggcaaag
     5521 acaatgcata aattgattat aaaatataac aaacaattga agatgctcaa tttgcgagat
     5581 ggtaagacat atactattag cgaagacgag cgtgcagata ttacgttgaa atcgttaggc
     5641 gaagtcattc atttagaaca aaataatcaa ggtacttggc aagcgaatca tacttctatt
     5701 aataaggtgc ttgttagaaa aggtgacctt gatgacatta cattacagct ttatacagaa
     5761 gctgattatg catcatttgc gtatccttca attcaagata cgatgacaat tggaccaaat
     5821 gcgtatgatg atatggttat tcaaagcttg atgaatgcca tcattattaa agattttcaa
     5881 tcaatacaag aatcacaata cgtacgcatt gtgcacgata aaaatacaga tgtgtatatt
     5941 aactatgaac tacaagagca actaacgaac aaagcttaca ttggtgatca tatttatgtt
     6001 gaagggatat ggctcgaagt acaagctgat ggtttaaatg tattgagtca gaatacagtg
     6061 gcatcgtcat taattcgctt aacacaagag atgccacatg cacaggcaga tgattacaat
     6121 acgtaccatc gttcgccaag gattattcac cgtgaaccga cggatgatat taagattgaa
     6181 agaccgccac agccaataca gaagaacaat acagtgatat ggcgttccat tataccgcca
     6241 ttagtaatga ttgctttaac tgttgtcatc tttttagtga gaccaattgg tatttatatt
     6301 ttaatgatga ttggtatgag ttcagtaacg atagtatttg gtattacaac gtatttctct
     6361 gaaaagaaaa agtataacaa agatgttgaa aaacgagaga aagattacaa agattatttg
     6421 gataataaat ctaaagaaat taataaagcg attaaagcac aacgttttag tttgaattac
     6481 cattatccaa cggttgctga aattaaagat atcgttgaaa cgaaagcacc aagaatatat
     6541 gaaaaaacat cgcatcatca cgatttctta cattataagt taggtattgc gaatgtagaa
     6601 aagtcattca aattagatta ccaagaagaa gaatttaacc aacgtcgtga tgaattattc
     6661 gacgatgcta aagaattgta tgaattttac acagatgtag aacaagcacc attaatcaat
     6721 gatttaaatc atggaccgat tgcatatatt ggtgcacgac atctcatttt agaagaattg
     6781 gaaaaaatgt taatccaatt gtcaatattc catagttatc atgatttaga gtttctattt
     6841 gtgacacgtg aagatgaagt tgaaacattg aaatgggcac gttggttgcc acatatgaca
     6901 ttgagaggtc aaaacattag aggatttgtt tacaatcaac gaacacgtga ccaaatttta
     6961 acgtcaattt acagcatgat taaagaacgt atccaagctg tgcgtgaacg cagtaaaagt
     7021 aatgagcaaa ttatttttac accgcaatta gtgtttgtca ttaattattg atcatgtcat
     7081 tttagaatat gtaaaccaag atttatcaga atatggtatt tcattaatct ttgttgaaga
     7141 tgtgattgaa agtttgccag agcatgtaga taccattatt gatatcaagt ctcgtactga
     7201 aggagaactg attacgaaag aaaaagaatt agttcaattg aaatttacac ctgaaaatat
     7261 tgataacgtc gataaagaat atatcgcgcg acgtttggcg aatttgatac acgtcgaaca
     7321 tttgaaaaat gcaattcctg atagtattac atttttagag atgtataacg tgaaagaagt
     7381 agatcagctt gatgtggtta atcgatggag acaaaacgaa acatacaaaa cgatggcagt
     7441 acctttaggt gtaagaggta aagatgacat tttatcattg aacttacatg aaaaagcaca
     7501 cggaccacat ggtttagttg ctggtaccac tgggtcaggg aaatctgaga ttatccaatc
     7561 atacatttta tctttagcta ttaattttca cccacatgaa gttgcgttcc tattgattga
     7621 ctataaaggt gggggtatgg cgaacttatt taaagattta gtccatttag ttggtacgat
     7681 tacaaactta gatggtgatg aagcgatgcg tgccttaaca tcaatcaaag ccgaattgag
     7741 aaaacgtcaa cgtttattcg gagagcatga tgttaaccat attaatcaat accataagtt
     7801 atttaaagaa ggtattgcga cagaaccaat gccacattta ttcattattt ccgatgagtt
     7861 tgccgaatta aaatcagaac aacctgattt tatgaaagaa cttgtatcaa cggcacgtat
     7921 tggacgttcg ttaggtattc atttaatact tgcgacacaa aaaccatcgg gtgttgttga
     7981 tgaccaaatt tggtctaact ctaaatttaa attggcatta aaagtacaag atagacaaga
     8041 cagtaatgaa attttaaaaa caccagatgc agcagacatt acattaccag gtcgtgcgta
     8101 tttacaagtt ggtaataatg aaatttatga attgttccaa tctgcatgga gtggagcaac
     8161 atatgacatc gaaggcgata aattagaagt tgaagataag acgatttaca tgattaatga
     8221 ttatggtcaa cttcaagcaa tcaacaaaga cttgagtgga cttgaagatg aagaaacgaa
     8281 agaaaatcaa actgagttag aagcggttat cgatcatata gaatctatta caacacgatt
     8341 agaaattgaa gaagttaagc gtccatggtt accaccatta ccagaaaatg tatatcaaga
     8401 agatttagta gaaacagatt tcagaaaatt atggtcagat gatgcaaaag aagtggaatt
     8461 aacattagga cttaaagacg taccagaaga acaatatcaa gggccgatgg tattacaatt
     8521 gaaaaaagct ggtcacatcg cgttaatcgg aagtccagga tatggtagaa caacgttctt
     8581 acacaacatt attttcgatg ttgcaagaca ccatcgtcct gatcaagcac acatgtactt
     8641 gttcgatttc ggtaccaatg gtttgatgcc agtcacagac ataccacatg tcgctgatta
     8701 ctttacagta gatcaagaag acaagattgc gaaggcgata cgtatattta atgatgaaat
     8761 cgatcgccgt aagaagattt taagtcagta tcgtgttact agtatttctg aatatcgaaa
     8821 attaactggt gaaacaattc cgcatgtctt tattcttatt gataactttg acgcagtaaa
     8881 agattcacct tttcaagaag tttttgaaaa tatgatgatt aaaatgacac gtgaagggct
     8941 agcattagac atgcaagtaa ccttaactgc ctcaagagct aacgctatga aaacaccaat
     9001 gtacattaat atgaaaacgc gtatcgccat gtttttatat gataaatcag aagtgtcgaa
     9061 cgtagtagga cagcaaaaat ttgcggttaa agatgtagtg ggtcgagcat tgttaagtag
     9121 cgatgacaac gtatcattcc atattggcca accatttaaa catgatgaga ccaaatcata
     9181 taatgatcaa attaatgatg aagtatcggc gatgacagaa ttttataaag gtgaaacacc
     9241 aagtgatatt cctatgatgc cagatgaaat taaatatgaa gattacagag aatcattaag
     9301 cttaccggat atagttgcaa atggtgcttt accaattgga ttagattatg aaggtgttac
     9361 actacaaaaa attaaattaa ctgaaccagc aatgatttca tcagaaaatc cgagagaaat
     9421 tgcgcatatt gctgaaatta tgatgaaaga aattgacata ttaaatgaaa aatatgcgat
     9481 ttgtatcgca gattcaagtg gagagtttaa agcttataga catcaagtgg ctaactttgc
     9541 cgaagaaaga gaagacatta aagcgattca tcaactaatg attgaagact taaaacaaag
     9601 agaaatggac ggtccgtttg aaaaagattc actttacatt atcaatgatt ttaaaacatt
     9661 tattgattgc acgtatattc cggaagatga tgttaaaaaa cttattacaa aaggaccaga
     9721 acttggcttg aacattttat ttgtcggcat tcataaagaa ttaatagatg cttatgataa
     9781 acagattgat gttgcacgta aaatgattaa ccaatttagt ataggtattc gtatttcaga
     9841 ccaacaattc tttaaattta gatttattca acgagaacct gttattaaag aaaatgaagc
     9901 atatatggtc gcaaaccaag cttatcaaaa gattagatgg tttaaatagc aatgaattaa
     9961 ataggaggga ggtatgttat gaattttaat gatattgaaa caatggttaa gtcgaaattt
    10021 aaagatatta aaaagcatgc tgaagagatt gcgcatgaaa ttgaagttcg ttctggatat
    10081 ttaagaaaag ctgaacaata taagcgatta gaatttaatt tgagttttgc actagatgat
    10141 attgaaagca cagcaaagga cgtacaaact gcaaaaacta gtgctaataa ggacagtgta
    10201 actgttaaag gaaaagcgcc caatacgtta tatatcgaaa aaagaaattt gatgaaacaa
    10261 aagcttgaaa tgttgggtga agatatcgat aaaaataaag aatccctcca aaaagctaag
    10321 gaaattgctg gagaaaaggc aagtgaatat tttaataaag caatgaatta atattgaggt
    10381 gaagatatgg gtggatataa aggtattaaa gcagatggtg gcaaggttga tcaagcaaaa
    10441 caattagcgg caaaaacagc taaagatatt gaagcatgtc aaaagcaaac gcaacagctc
    10501 gctgagtata tcgaaggtag tgattgggaa ggacagttcg ccaataaggt gaaagatgtg
    10561 ttactcatta tggcaaagtt tcaagaagaa ttagtacaac cgattgctga ccatcaaaaa
    10621 gcaattgata acttaagtca aaatctagcg aaatacgata cattatcaat taagcaaggg
    10681 cttgataggg tgaacccatg atgaaagatg ttaagcgaat agattatttt tcttacgaag
    10741 aattaacaat tttaggtggt agtaaattac ctctcgtaaa ttttgaattg tttgatccat
    10801 caaattttga agaagctaaa gcggctttaa ttgaaaagga attagtaaca gagaatgaca
    10861 agttaactga tgcaggtttt aaagtggcta cattagtcag agagtatatt agcgcaattg
    10921 taaatattcg aattaatgat atgtattttg caccatttag ctatgaaaaa gatgaatata
    10981 ttttgttaag ccggtttaaa aataatggat ttcaaatacg aattataaat aaagacattg
    11041 catggtggtc gattgtacaa tcatatcctt tattaatgag acaagaaaag tccaatgatt
    11101 gggactttaa acaaattgac gatgaaacat tggagaactt aaataatgaa agtatcgata
    11161 cgattgggcg tgttttagaa attgaaatat acaatcatca aggtgaccct caacaaagtt
    11221 tatataacat ttatgaacaa aatgatttgc tactcattcg atacccatta aaagataaag
    11281 tactgaatgt tcatattggc gtcattaata catttatacg agaattattt ggattcaata
    11341 ctgatgaaaa tcatattaat aaagcagagg agtaatgacg ttgagtggaa aaattagtgt
    11401 taaagctgaa acgattgcac atgttgtaaa agaattggaa agcattagtc aaaagtatga
    11461 tgaaatagct caaaactttg gaaaaattgc gcaattaaat tactacagta gtgaaaaagc
    11521 ttcacattct atggaaaacg gctatagtag tgctgcaaca gtcattagtg gtctcaaagg
    11581 tccattgagt acactcggtg gtggtgtcat gaattcagca caaaagttct ttgaagcaga
    11641 tgaacattgg ggtacggaat ttgccaagct ttactataat attgagggat aggtgcatga
    11701 catgacaaaa gatattgaat atttaacagc tgattatgac aatgaaaagt catctatcca
    11761 aagtgtaata gatgcaatag aggggcaaga cttcttagat gtagatacaa caatggatga
    11821 tgcggtaagc gatgtcagtt ctttagacga agatggcgca atatcattaa caagtagtgt
    11881 agtaggtcca caaggatcta aattaatggg ctattatcaa aatgagttat atgattatgc
    11941 atctcaatta gattcgaaaa tgaaagaaat tattgacacg ccatttatag aagatataga
    12001 taaagcattc aaaggtataa cgaatgttaa attggaaaat atattaatta aaaatggcgg
    12061 tggtcatggt agagatacct atggggcttc tgggaaaatt gcaaagggag atgccaagaa
    12121 aagtgacagc gatgtttata gcatcgatga aatattaaaa tcggatcaag aatttgtaaa
    12181 agtaattgat cagcattaca aagaaatgaa aaaagaagat aagaaattat ctaagagtga
    12241 ttttgaaaaa atgatgactc agggcgcttc ttgtgattac atgacagtag ctgaagcgga
    12301 agagctagag gagcaaaaga aaaaagaaga agctatagag attgcagcac tagctggtat
    12361 ggtagtttta tcttgtatta atcctgttgc tggagcagta gctattggtg cttattccgc
    12421 ctattcagca gcaaatgcag ccacaggaaa aaatattgta actggaagaa agctatctaa
    12481 agaagaacga atcatggaag gactttcgct tattccattg ccaggtatgg gcttcctcaa
    12541 aggtgctggg aaaagtttaa tgaaattagg cttcaaaggc ggagaaaaat ttgcagttaa
    12601 aacaggattg caaaagacaa tgcaacaagc ggttagtcgt atttcaccta aaatgggaat
    12661 gatgaaaaac agtgtgttga atcaatctcg taactttgct caaaatactc atgttggaca
    12721 aatgctgagt aacatgcgtg gtcaagcaac tcatactgtt caacaaagta gaaattggat
    12781 tggacaacaa gcgcaaaatg tcaaacgaat agtgaataat ggacttgata aagaaatagc
    12841 acatccattt aaacaacaac ttgcaccagc gggaatgggt ggtataaaat ttgctgaaac
    12901 aactactttg agaaacatgg gtcaaaacat gaaacgtgct gttacaccac aaaatcacgt
    12961 gacacatggt ccgaaagata gtatggtgag aagtgaaggt aaacatagtg taagtagcca
    13021 tgaaataaat tcatcaaaat atgttgaatc accaaactac accaaggttg aattcggaga
    13081 acactatgca agacttagac ctaagaaact aaaagcaaat atagaatata caacaccaac
    13141 tggtcacata tatcggaccg atcataaagg gcgcataaaa gaagtttatg tagacaatct
    13201 ttctctaaaa gatggcggtc gtaataacca tgcacaaaga actgtggggg gagaggatag
    13261 attaccagac gatgatggag gtcatttaat cgctagaatg tttggtgggt caaaagacat
    13321 agataacctt gtggcacaaa gtaaatttat caaccgttca tttaaggaaa atggacaatg
    13381 gtataagttg gaaaaactat gggaaaaagc aatcaagtct gggaagtcaa tagaaaatgt
    13441 taaagttgaa gttaaatata aaggtaatag tcaaaggcca accgaatttt cagttagata
    13501 taatattaat ggtgagcagt ttttaaaaaa tataattaat aaataggagg attaacatga
    13561 ctttcgaaga aaaactaagt caaatgtata acgagattgc gaataagatt agcagtatga
    13621 taccggtaga atgggaaaag gtatatgcaa tggcatatat agatgatgaa ggaggcgaag
    13681 tgttctacta ttacacagaa cctggaagta atgaattata ctattatact agtgtattaa
    13741 ataaatatga tatatcggaa tcagaattta tggattcagc gtatgagttg tataaacaat
    13801 ttcaaaagtt aagaaatata tttaaaaaag aaggacatga accatggaca tcatgcgaat
    13861 ttgattttac aaatgaaggt aaattaaaag tttcatttga ttatattgat tggataaata
    13921 cagagtttga tcaattgggc cgtcaaaatt attatatgta caaaaaattt ggggttatac
    13981 cagaaatgga atatgaaatg gaggaggtta aacaaatcga gcaatatatt aaagagcaag
    14041 aagaagaata gacaatctta atttgagact aaacaataaa gctttgttta gtcttttttg
    14101 gcggttaagt aaaaagcaat agagaccgta aagttgatgc tcatcaaata ataatataaa
    14161 gataatttta ggtttttaaa cttttaatcg aaaagaatct aagtatatat agctttaaat
    14221 acgtaatatg ctatttaaaa gataatgtat aggagataga tatgactact aaagaaaata
    14281 ttgatactct tcgaaagcca ggtgcacaag ccttaagttt aatatcattg tttttgatac
    14341 ttttttcatg tctaactttc ttttttggtt tagattatga aaggtttcca aacta
//
LOCUS       DAMZLE010000061        14108 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMZLE010000061 DAMZLE010000000
VERSION     DAMZLE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.061"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(224..730)
                     /locus_tag="POB92_001568"
     CDS             complement(224..730)
                     /locus_tag="POB92_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579070.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(866..1666)
                     /locus_tag="POB92_001569"
     CDS             complement(866..1666)
                     /locus_tag="POB92_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6579071.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            complement(1650..2369)
                     /locus_tag="POB92_001570"
     CDS             complement(1650..2369)
                     /locus_tag="POB92_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6579072.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(2424..3374)
                     /locus_tag="POB92_001571"
     CDS             complement(2424..3374)
                     /locus_tag="POB92_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6579073.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3523..4410)
                     /locus_tag="POB92_001572"
     CDS             complement(3523..4410)
                     /locus_tag="POB92_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6579074.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKEKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4661..5296)
                     /locus_tag="POB92_001573"
     CDS             complement(4661..5296)
                     /locus_tag="POB92_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDI6579075.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQVKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(5687..7348)
                     /locus_tag="POB92_001574"
     CDS             complement(5687..7348)
                     /locus_tag="POB92_001574"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI6579076.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKCEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7345..7761)
                     /locus_tag="POB92_001575"
     CDS             complement(7345..7761)
                     /locus_tag="POB92_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDI6579077.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLDVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(7854..7961)
                     /locus_tag="POB92_001576"
     CDS             complement(7854..7961)
                     /locus_tag="POB92_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579078.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(8038..9048)
                     /gene="adhP"
                     /locus_tag="POB92_001577"
     CDS             complement(8038..9048)
                     /gene="adhP"
                     /locus_tag="POB92_001577"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDI6579079.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(9547..10068)
                     /locus_tag="POB92_001578"
     CDS             complement(9547..10068)
                     /locus_tag="POB92_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDI6579080.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(10096..11391)
                     /locus_tag="POB92_001579"
     CDS             complement(10096..11391)
                     /locus_tag="POB92_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6579081.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(11431..11907)
                     /locus_tag="POB92_001580"
     CDS             complement(11431..11907)
                     /locus_tag="POB92_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579082.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(12076..12612)
                     /locus_tag="POB92_001581"
     CDS             complement(12076..12612)
                     /locus_tag="POB92_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDI6579083.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(12823..13761)
                     /locus_tag="POB92_001582"
     CDS             complement(12823..13761)
                     /locus_tag="POB92_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6579084.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         4458 a         2678 c         1830 g         5142 t
ORIGIN      
        1 aaacctttat gattaaagta atcttctata ttttctagta aaattttatc gttaatacgc
       61 attggtagta ctcctttaaa atgaaacatc tttatttatt gtattggttt aatttcccct
      121 tcgcaaatca taaaaattaa tattcactgt attatgccat gtaataaaat aaccgacaag
      181 tattgcgatt taacttgtcg gttaagcacg attaatttaa ttattatttt tccaaatcaa
      241 tacgatataa ttttatgttt ttatcttttg gtgctgaggc aaattgataa gtcgttttgc
      301 ctttttcaat atatccaaaa ttaccagcaa ctttgctata ttccgtatgt gttactgctt
      361 tgccaacata atttttaaca gcttgatatg ttggaccatt agcatcatta tacatcattg
      421 aaatatgagt tactttacca ttgtttttct taccatcagt agttactaat aacatacctt
      481 ttttagtatg gaattcgtaa taatgttcag ttccatcttc attgtaagaa tacataggat
      541 ttttgtactt agttaaaaca tctttaatag attcgccaat tttcacattt tctaatgact
      601 gatcaccctt aattaattgt ttaacagtat caatagagtt gccacttgct gcttcggcac
      661 tagtgttcac taagcctact cccattacaa cagaacatgc aattatactt gccgttagta
      721 gctttttcat aagcactctc tccttttatt tatatcgtct tgtgctcaca accattatac
      781 aacaatcatt tctaaaacaa cagtcatatt acaattatat tacaaataat aattactact
      841 tttatatttc acttatcact aaaaattaaa catgcttttc atcgtgatgt ttgttgataa
      901 atgaagctgt atcttcgacg aatctttctt gctcttcaac aaatgggaat gcacttgatt
      961 cttgatatac ttcaaagtcc gcattttgga ttaaatcagc tacttcttta gcttccaatc
     1021 ttgttgttct ttcaccgaat tctcctgcaa taatttttgt cggtactacg acatttctat
     1081 aagtttgaga aatatcagcg tttttgaata cctctttaac tgcttgaatt tcttctttag
     1141 ttgatatttc atttgtatct acgacatgtt taaggaatcg attcattttt ctcggacgat
     1201 aatacttacg cttatttaaa aatttatctt gtttttcagg atcccagttt cgaataatat
     1261 gggcatattt tctaaataaa cgttcttccg gtaattcacc ttcaatagat gttggattta
     1321 ccaacataag tgatgatgta aattcaggat aacgtactga aatatccgca ccaatgattc
     1381 cacccatttc atggcataca aatgcaactt cttcaatgta taaatattta agtaattcaa
     1441 caatgtcatc agaaaaatct tttatttcaa tgtgacgagg tttatcagaa tagccatgtc
     1501 cacgtaaatc aattaacaca acttgaaatg attttgctaa ttgtgctgct aatttattaa
     1561 acacagaata attatcaagt acagtatgaa tcaatacgat aggatagcct tcacctaatg
     1621 tactgtaatg aatcgatgtt ccatcttttc tagtaaatag atccataatt ttctccttta
     1681 ttatcatttt ttattggtaa aatggttaat aattcttcca ctgttgtaat tgtaaaatca
     1741 acttcttctt cgagtggttc aatttcagca tcttcttgtt tataccatac actaaccatg
     1801 cccatagctc gtgctggagc tacatcattt aacgcatcat cgccaacata cataatttgc
     1861 tcaggtaata cccctagttg atcaatcata tcttcaaaaa ttttaggatg tggtttacga
     1921 aaaccaactg tttctgatgt tgataaataa ttaataacat gcatcaaacc aagtgaatgt
     1981 aatcgaaatt gtttaatctt agatttacca tttgcgataa caccaatttg aaagcctttt
     2041 tgcgatagct tttctagtgt atacaaagta tcgtaataag gaaatacata acgataaaaa
     2101 tgcatttcaa aatcattaaa taagtctttc caagttaacc tatctacatg gaaacgttta
     2161 atgatttctt tatataaatc aggtttatca ttatcttcat cgtcatctag ctcaataaat
     2221 gcttttttaa aatctgccag ttgaacatga attaagtagt catgaaatcg ttcatattgc
     2281 tcttcgataa atttctctcg agatttaact ctgtccaaca acgtaccttc taaatcaaat
     2341 acaactgctt taatttgatt caaatccatc gcttatgccc tctttatgtt tatagttatt
     2401 tttattttag caaatgtcat gtgctagtgt aaccgtttcg actgagatat tagcatgtaa
     2461 aataacactg gtataccaat taatgcaatg ataatacttg ccggtatttg tactggtgac
     2521 aatatatggc taccaataaa atctgccata accattattt gtcctccaat tacaatattc
     2581 aaagccaatt gtcttcctaa tgattgtttc cactgccatt ttctaatgag ttgcggtatt
     2641 accataccaa tgaatccaat gatgccaaca tatgcgacat ttatcgccgt tatcatagaa
     2701 gctatacata acgcgatata tgtaatcaat tgaacattta agcctagtga ctgacttttt
     2761 agttctccta attgcaataa ctttagttga ttaagaatga taaatatacc aaacagtgca
     2821 atgataaatg tgattgctat tatggataca tttgagtatt ctgcatcacc aaagccacca
     2881 aacaaataat tggcaattgt atttaatttg cgaggtttta ataaaatcaa aaaatataga
     2941 agtgaattga ataatgcacc aatcattaaa ccacttaata ttaagattct aactggatag
     3001 ccttggctca ataccgacgt aatgactaat acagttatta atgttatcaa actaaatgtt
     3061 attgaaaata caggaatcca taacgttgtt aaacctaaaa ataatgctaa tcctgaacca
     3121 aatgtagcac cgcttgccaa tcctaatgta aagctatctg ccaatgcatt atttaaaact
     3181 gtttgaaata taagacctgc aactgttaat atcatacctg tcaacaatgc ttctaataat
     3241 ctcggtaatc taacattgat taaaatagac tggttaaata catcattgat atcccccaac
     3301 tgccaaaata gatatatgct agttgttata atcaatatga ctatccagct caataatact
     3361 ttattaaatg tcatagcatt ctcttttctt tcatcgataa ttacattcat tatagcaatc
     3421 acatactcgc gttgctatca ttcactaaat atattttaaa aaccccttat ctcaaacatg
     3481 tattttttta catgtagcaa taaggggcat aattagaatg gtttattttc tataaattgc
     3541 atctcttaat tcttttaatc cttcatcaat acgtggaccg ggtcttgata cttcatcacc
     3601 atttacaact tcaatacgtg tattcttgac agcattaatt ttattgaatc cacctctttt
     3661 tttgatgata tccatataat ctgatcttgt cttagcttcc gtcgaaatta atatatctgg
     3721 gttcttttta ataatacttt ccttcgttac agggttccaa ccattaatgt cactataaac
     3781 attttgggct tctaattttt ctaacatatc attgaaaaat gtatgctttc ctgctgtata
     3841 tatttcaggc tttgatgaaa cctcaataaa tacttttgat tttttatgat gagcaggaat
     3901 tgaatctatg actttatcga tattatcttt agtttcctca acaagttgtt cagcctgctt
     3961 atcatgatgc gttaatttcc caatttgctt aaatgtgttg tatgtttcat caattgattg
     4021 tgcatcttta acatacacta ctttgatgcc ttgtttctct aatgatgaca atactttatt
     4081 agcagttgcc ttttgcgact catgcgcaag aattagatct ggcttttcct ttaaaagttc
     4141 ctctttattt agattcaaag catcaaattg tttcttaccc tctttcacat cttttggata
     4201 atcatcaacc gttgaaacac caactatgta tttacctaat cctaattcat ataaaatttc
     4261 agtattacta ggcattaacg aaacaattct atggtacggc gatttagacg agccattatc
     4321 attgctacct tgtttatcat tatctttcat accacagcca ctcaggacaa gcataaaaat
     4381 caaaataaaa gcaattaacg atttcttcac actatcacat ctccttttta aagcctcttg
     4441 agctttagcg attagaggct tttatgcagt tgtgcgcaag caagccaact gcgttcctct
     4501 tcacctttag cgattagagg cttttatgca gttgcgcggt agcatccaac tgttttttac
     4561 tttatatgct tctatttatt atataagtcc acatactaaa agaaaatagt aaattatttt
     4621 ttatatttca tataaactca tctatctaac ttcacagcgt ttacttttta aagtaaggtt
     4681 ctaaaaaatc ataattcttt atatctttgt ctctaaagta atgtttacca aatacatcta
     4741 acagagcatg aaattcatta gcatcttgat ttgtaaaatg atttggtaat gtgactactt
     4801 tttttaattg atcataattt ttagtgtttt catatcctaa tttatcatat atttttcttg
     4861 tatagctatc tggaataaat tcaatacgtc cgaatatata aacaagtaag acatctgctg
     4921 tttcacttcc aatacctttc aaagataata attcttttct taatccacct ttatatcgct
     4981 cattaatctc ttgataattg aaatgatgtc gtgctaacca tgttaataat gttttaatcg
     5041 tcagtgattt acttttataa aagccacttg aatgtatcaa tgattgtaac gtttcaatag
     5101 gtagttctaa tatatgattt ggattaaaat gcgtgtgttc tttaacttga ttcaatgcaa
     5161 tttctgcatt tcgccatcta gtattttgaa ctaaaattgc acctaacatc atttcaatgt
     5221 cattatcagc aggccaccaa aattgtggtc cgagatgttc atataaaact ttatataatt
     5281 catcagttcc taacatttac accctccatt ttagagggag tgggacagaa atgatattat
     5341 cgtaaaattt atttcgtcgt cccaccccaa cttgcattgt ctgtagaaat tgggaatcca
     5401 atttctcttt gttggggccc cgccggcaag gttgactaga attgaaaaaa gcttgttaca
     5461 agcgaatttt cgttcagtca actactgcca ttataacttt gtagagcata gaacattgat
     5521 ttatgtctca ggctctctta tgcagttgtg cagtagcatc caactgcgtt cctcttaacc
     5581 ttattcacat actaagcatt ccccaatatg tcacatacct aattaaaaac actccaacac
     5641 aaagtatctt tcgagaaact ctacgttgga gtgtatataa atgttcttac attgattcag
     5701 gtgcgcttac accgaccatt gccaatgcat ttttcaatgt aattctgacc gcttcaatca
     5761 tagcaacatg tgcttttgtt ttttcaatat catctgttaa cactttttca gcattgtaga
     5821 atttatggaa atgagaagct aaatcttgaa tataattagt aattctatgt gctgatctat
     5881 gttctgcagc actttcaatt gtaggctcaa agtcagctac ttttttcaac aattcaatcg
     5941 ctttttcatt agtaattgtt gtaaaatcat tcgcagcagt cacttcaata ccttgctctt
     6001 tcgcttgttt taaaattgaa caaatacgcg catgtgcata ttgagcatag taaactggat
     6061 tgtcttgaga ttgctctttc gctaattcca tatcaaaatc aaagtgacta tcaggactac
     6121 gcatagttaa gaaataacgt gcagcgtcaa cgccaacttc gtccataatt tctcttaatg
     6181 taatcgcatt accagtacgt ttactcatct tcacttcttt accattttcc attaaacgaa
     6241 ccatttgcat gatttgaatt tctaaacgat tactatctac accaaacgtt tcaagagatg
     6301 ctttcaaacg attaatataa ccatgatgat cagcaccaaa taaatcgatt aaaatgtcat
     6361 taccacgttt aactttatcg aagtggtacg caatatctgg taagaaatac gtatatgtac
     6421 cgtcattttt aattaagact ctgtctttgt cgtctttaaa atcagttgta cgtaaccatg
     6481 tagcgccatc agcttcatac gtataaccta attctttcat ttttgctaaa acttcaagaa
     6541 tttcgcattt ttcatataaa gatgtttcac taaaccaatt atcaaaatgc gtattgaact
     6601 ctgctaaatc atttttcaat ttagccattt cgtattctac gcctaatttt ctaaattctt
     6661 tcaaacgtgc ttcttcagaa taatctttaa tttcagggtg tttctctgct aaatctttac
     6721 ctatttcaat aatatctttt ccattatagc catcttctgg cattgaataa ctattgtcac
     6781 ctaaagcttc aaagaaacgt gtttcaatcg aacgcgctaa gttagtaatt tgattaccag
     6841 catcattaat ataatattca cgtgttacat tatagccagc tgcagttaaa atattagcta
     6901 aagcatcacc aactgctgca tttctagcat gaccaatatg taaatctcct gtagggttag
     6961 ccgaaacata ctcaagcaat acattttgac cttttgattc atttacatgt ccaaattgat
     7021 cacctttttc aattgcttca ggaataattg ctgttaaata ctgattatct aagtaaaaat
     7081 taatgaatcc tggaccagca atgtcaattt gttttacatg tgctttttca gtatctaagt
     7141 tatcaacaat cgcttgagca atttcacgag gattacgctt tgcaatctta gtcagtacca
     7201 tcgcaatatt agtagcataa tctccatttt ttgtatcttt aggaacttca attttaatat
     7261 caggaatctc atctgctaat cctgctttgt taatacttgc tgcaatttct tctactaatg
     7321 tttgtttcac ttgatcaata atattcattc gcctatctcc ttatagttaa tttcatattg
     7381 ataagaaccc attttttcat tatcttgata taattcataa tgaatcttta gcttgccacc
     7441 gtctccactt acgaaatgta aaatgcgtaa tgttttaaca tctagtggaa ttcgtccagc
     7501 cgatatatcg taaaaagttg ttgtcgtttg tccttcaaca aaatgcaaat tcatattaat
     7561 gtcgccttta cgaatcaact taacgctatc atcatccact tttattgtaa cattaactgt
     7621 tgcatcctca atttgttcta cataccgaat aaagttagat tgcctttgtt gccaagttcc
     7681 ttcagtagta aattcaaatt tttctttttc gttgtgctgt ttcaacactt gctttgtttg
     7741 aatacttact tttttatcca atttactact caccttaatc ttcgcataat agaaatatta
     7801 tagcataaaa taaggtcatc ttgttatgcc ttcttaaagc taaataggtt caattaatta
     7861 acaattccat acttaatcaa cgcttcatat atgtaagcca ttttataaaa tacaaacaac
     7921 acctcaaaac caaaaaacac gagattttca ctatctttca tgaaaacctc gtgtttgatt
     7981 ataatgatag tgttcagagc actaccttct tcatctatat ttaatcaatt aatattttta
     8041 aaatttaata accattctac cagttatagt accattttcc atttcttcaa aaatatcatt
     8101 gatttcttct aattttctta attgaacttt aggtgttact ttattttcag cagcaaattc
     8161 aaacgcttca cgtaagtctt gtcttgtacc aacaagtgaa cctactactt caataccgtc
     8221 aagcactaat cttgggatat ctaagttcat tttatcaaca ggtaaaccaa cagcaacaac
     8281 tcttgcacca gcttttacaa catcaaccgc ttggttaaat ggcgtcttag caactgaagt
     8341 taccactgtt gcatctaatc ctttgttatc agttaatttc ataacttctg caactggatc
     8401 aacatcttta gaattaataa tagcatctgc acctaattct ttcgcgaatg ctaatttatc
     8461 atcattaatg tcgaaggcaa caattttagc ccccataacg tttttagcat attgtaaagc
     8521 taggttacct aaaccaccta taccaaaaac acctaaccat tgaccaggtt ttacattact
     8581 tacttttaca gctttataag ttgtcacacc tgcgcatgta atagaagacg ctgctgctgg
     8641 atctaatttt tcaggtactt tcacagcata gtctgcagta acaataactt gttcagccat
     8701 tgcaccatct actgtataac cagcattttt cacactacgg caaagtgttt cacgacctgt
     8761 tgtacaatat tcacatcttc cacagctttc gaacatccaa gcgatagaca cacggtctcc
     8821 aatttttaat gattctacat cttccgcaac ttcgatgact ttaccaatac cttcatgacc
     8881 taaagtaacg cctgtaacat caccaaaatc agcattctta acatgtaaat cggtatgaca
     8941 aacgccacaa tattccgttt gtaccaacgc ttcaccaggt tttaaagctc ttaacttttt
     9001 gtcctcaata cttactttgt gatctttcgt tacaactgct gctctcataa taatgtcctc
     9061 cttatagtca ctccagtatg tatgatatta attggcgatt actttcaaaa aagaaaacga
     9121 ataattatag atgtaatttt tatttgtaat agacgccgta ttaaaacata ctactgtgac
     9181 cttgttttta gttagatatc aaccgttaac ttttaacgtt tccctagtta atatgttaaa
     9241 agaaatgaca taatcgatat gctaacgtgt aatttttact tcacatatat ttatttaaag
     9301 ataatttata atactcttat cttcactctt attataatgt gaattaattc acaaagtata
     9361 tagctatgat caaaaaaatg tgtcagttat tcaaattttt tattaactac agattaattt
     9421 aatttcaaaa aaattaaaaa aactcccaat caatctaaga ctttattttc acctcaagat
     9481 ttacgtcgtc attgagaaat gaatcatatc tttatagatt aattgggaaa ttttagtttc
     9541 taatatttaa ttaaatgctt gatgtaaagc atcagaagaa tgcttccaca taccttcatc
     9601 atggttcatt aaaaagtctt ttaaaatagc tttattctcg tcaccaattt gatcaatgat
     9661 aatatgatgt ttcattgatt tatccatcat attaacatgt tcaggcatac ttttataacc
     9721 acgacgaatt gctttattca ctaataaata gcacgctgtt aaaccagcgt aatatggtcc
     9781 ttgttcatta cgttctgttg ttacccaaca caaccaatat tctttagctt catcgccttc
     9841 aacagcagct ttatcagtaa tccatttcac acctttttca actttggcac gagcatgcat
     9901 gcctccaata tcaataaatg cttctttgtt agcaacatcg ataaaaactg gtgcaatatt
     9961 gtctaagcta attgaaccaa tatttgtacc tttatgacca tctagagggt cattttttat
    10021 gatattaaaa ttaaagcctt ttttctcaga caatgatata acctccatat ttaacgataa
    10081 caattttagc cctgtttaat taagctcatt aatcaaattt tcaatagctt ctctaattgt
    10141 cttatcttta atctttgcaa ccatttctct aggataatat aatttatagt cttggcgatt
    10201 aatgcctgta atactatgaa tgactaatga ttgattgctt atttctctaa tcgtaccatc
    10261 ttgtcttaat aaatgaatcg gtttgcgatt tgaccccggt cgatcatagt catatggcaa
    10321 atccgaaaat gcttcactca caaaataata atctggatta ataccacctg cttcaaacag
    10381 ttcttgcagt tctgatattg taataattga gccatcaaat ggaatatatt taaataagtc
    10441 tcgattaata aatcgacttg ctaaatcact taaaatggca tcatcttctt ttgtccattt
    10501 ttccaaataa tacgtaacta ccgcttcatc gagttcaaca tattgttcaa tcgtaactgt
    10561 ctcttcaaaa aatggaataa aatcatgtgg atgcaactta aattcatagc cttcattata
    10621 aagctgtttt gcgcgtttca aacaattatt aagcagcact tctccaccac gactaactgg
    10681 gtggaagtaa atttgccaat acatttgata acgactcata ataaagtttt caactgcatg
    10741 cataccactt tctttgatta gtacttcatc tttagaaggt cgcattaatc ttaaaatacg
    10801 ctccatatca aaagcaccat atgatacacc tgtgaaatac gcatcacgtt gtaaataatc
    10861 cattctatcc gcatcgattt gtgacgaaat catcgaaatg accaatttat tatgatgcgt
    10921 tttattaatt acttccgcaa cttctcttgg aaactcaggc gccactttac gtaatacagc
    10981 attcacctca gtatctccag taataatcgc ttgtgtgtat gcttcatggt ctgtattaaa
    11041 tattttttca aaactatgtg aaaatggacc atgtcctaaa tcatgtaata atgcagcaca
    11101 taatgccaac ggtctatctt tattgtccca tgcatcatga ccaataaatg actcatcaat
    11161 taatcgtcta actatttcat acacacctaa agaatgtcca aagcgactat gttctgctgt
    11221 gtgaaaagat aggtacagtg ttcctagttg tctaattcga cgtaaccttt ggaattcctt
    11281 agttttaatt aaatcccata tcaattgatc ttcaacatga atatatcgat gtatcgggtc
    11341 tttaaaaact ttttcttcta ctaattttaa atctacatat gcgttagtca ttattcccct
    11401 ccttttcgtt taatataata tttaatttac ttaaaatgct ttgtacataa gtgctaagtc
    11461 taacttttcg ccatacattt ctggctcata agagcgtaag attgtaaaac cttgctcttt
    11521 atagtaagct actgcttctt catttttatt atctacttct aagtaaacac cttcaaattt
    11581 atcttcaaaa cgtgataatc cttcatttaa caatgctgta ccataacctg tatgttgcga
    11641 ttctggttta acataatgag ctgataaata taattcttca ccgtaaataa agttagcaaa
    11701 gccaacgatg tcattacctt cttcaacgac taagaataat tgttcttgaa gtcttttctt
    11761 taaatgatgt tcattatatg aagcttctaa caagtgatta actgttgtcg cagcgtatat
    11821 atttaagtat gtattaaacc aagctttagt tgcgacatct ctaatttgaa caacatcttt
    11881 ttcagttgct tgtcttacct tgaacatgac tttctcccct tattaacaag ttttaataac
    11941 ggcattatac cacaacttgc tcaatactta ataaacaatg attgtctatt caatttatat
    12001 atttatattt tccgttaaaa ttaaaaataa aaaataacga agcaaaaaat cacttcgttt
    12061 agtatgaggt atgtcttatt gcaatatact attccactca gttgcacgtg ctaaggcata
    12121 gttgtctttc atgatgtcac caggcttttc agcagttcca ataatataac catttaaagt
    12181 ggcacctaaa aagtctaaac tatatttcat ttgcgtaatt gctggcttcg cttttatttt
    12241 tggacaatct ccaccaacta aaataactct aaaatccttt tcggccattt gtgccttaaa
    12301 attaggatat cgtttatctt gtaatgtttc tgaccaatgt tcgataaatg ctttcaatgg
    12361 tgctgaaatg ctataccaat acactggtga tgcaaaaata attgtatcac tagccaatat
    12421 tttatctaga atcggcaaat agtcatcgtc atatgaagta atagtctctg ctgtatgtct
    12481 cacgtcacgt atcggtttaa actgatgttg tgtcacgtca atccattgat actctaaatc
    12541 ttgcaaagcg aattttgtta attgtgcagt attaccgttt ggtctactcc caccaaacaa
    12601 aacagtaatc attttagcct aacctcactt ttgattaata aatatctgtg tttttcgtta
    12661 cctaattata ctatcataag ctttgcctac cgaatagtaa aacgcttaca acttttatat
    12721 aaatttgacg aaatttcgtc atgccttata taacgtcgtt tgtgatacgg ggctaattca
    12781 tgatgaaatt agatacatat atcaccatta aatacaattc atttagtctt caatcggaaa
    12841 cagttcatcg atatattgaa tctcatcatc tgataaaacg atatctgcag ctttaatatt
    12901 ttcaacgact tgttctgcac gttttgcacc aggaataatc acatcgatag ctggtctcgt
    12961 taaataaaat gctaatacaa tgttcgcaat tgaagtttga tgtgctgcag ctatgctttc
    13021 caaagctttt acgcgacgca cattttcttc aaatacacct ggtttaaaat cacgacgtgt
    13081 agtacgatgg tcactaaatt tagtgttctc atcatatttt ccagctaaaa taccggatgc
    13141 taatgggaaa tatggaataa atgtgatttg gtgatcaaca caatattgta atactgcctc
    13201 attttcgcga tgcaataaat tatattctaa ctgtacaaca tcaacgtaac catctttatt
    13261 tgcttcttta agttgatcta atgtgaaatt tgatacacca attgctttaa tcttcccttg
    13321 ttccttaagc tcttgtaatg ctgcaactgc ttgatctttc ggagtgttgt tatccggaaa
    13381 atgaatataa tataaatcga tataatcagt ttgtagacgt ttcaaactat tctcaacttg
    13441 ttgttttaaa tattccggtt gattgttctg atgtacttct tgattttcat caaattcatg
    13501 agaccctttc gtagcaattt taatttgctc tcgcggatat tctttaacaa cttctccaac
    13561 caattcttct gatcgttctg gcccataaat atatgccgta tctaataaat taataccatg
    13621 attaatggct tgacgaacaa catcttttcc ttgttcttca tctaagttcg gatataaatt
    13681 atgcccacct actgcgttcg tcccaagtgc gattggaaac acttcaacat cagatttacc
    13741 taagtttaca aattgcttca ttagacccag ctccttatct ttataacttt tccactatca
    13801 cactatcatg atttaataca acttccaaat gttagaatta gagcttgaat ttaagcggtg
    13861 taataggaaa acgttatata aaactcagtt aaaataagta ttgcttaaat tgatatgcat
    13921 atagcgcgat tgctcgttga cctttaaatt acatgtactt ttgaatcaga aattgaaaaa
    13981 catttaagaa cgacaatatt aaagccattg caaatttaat ttggtaattt ctattggttt
    14041 ttaagtatga catgaatgtt tttatctata attaaagcta tattaactag aaaaaaaccc
    14101 attataac
//
LOCUS       DAMZLE010000062        13507 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMZLE010000062 DAMZLE010000000
VERSION     DAMZLE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.062"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(64..297)
                     /gene="nrdH"
                     /locus_tag="POB92_001583"
     CDS             complement(64..297)
                     /gene="nrdH"
                     /locus_tag="POB92_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI6579085.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            495..1856
                     /locus_tag="POB92_001584"
     CDS             495..1856
                     /locus_tag="POB92_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDI6579086.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1853..2872
                     /locus_tag="POB92_001585"
     CDS             1853..2872
                     /locus_tag="POB92_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6579087.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            3005..3667
                     /locus_tag="POB92_001586"
     CDS             3005..3667
                     /locus_tag="POB92_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDI6579088.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(4016..5713)
                     /locus_tag="POB92_001587"
     CDS             complement(4016..5713)
                     /locus_tag="POB92_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI6579089.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(5713..5931)
                     /locus_tag="POB92_001588"
     CDS             complement(5713..5931)
                     /locus_tag="POB92_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDI6579090.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(5960..6082)
                     /locus_tag="POB92_001589"
     CDS             complement(5960..6082)
                     /locus_tag="POB92_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579091.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(6413..6964)
                     /locus_tag="POB92_001590"
     CDS             complement(6413..6964)
                     /locus_tag="POB92_001590"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6579092.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            7329..7955
                     /locus_tag="POB92_001591"
     CDS             7329..7955
                     /locus_tag="POB92_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDI6579093.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            8126..9238
                     /gene="pdhA"
                     /locus_tag="POB92_001592"
     CDS             8126..9238
                     /gene="pdhA"
                     /locus_tag="POB92_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDI6579094.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            9242..10219
                     /locus_tag="POB92_001593"
     CDS             9242..10219
                     /locus_tag="POB92_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6579095.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            10310..11602
                     /locus_tag="POB92_001594"
     CDS             10310..11602
                     /locus_tag="POB92_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDI6579096.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            11606..13012
                     /gene="lpdA"
                     /locus_tag="POB92_001595"
     CDS             11606..13012
                     /gene="lpdA"
                     /locus_tag="POB92_001595"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6579097.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            13180..13455
                     /locus_tag="POB92_001596"
     CDS             13180..13455
                     /locus_tag="POB92_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDI6579098.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
BASE COUNT         4600 a         2033 c         2516 g         4358 t
ORIGIN      
        1 taatttaaaa atcgatattt aatttatgat agctataaat gatgaagttg atttagatga
       61 aaattaatct tggatattta atactttgtt gatttcatca agatcaacat ggtacattgg
      121 attaccattc aacaaaataa acggagttga aaaagcatca aaatctatca tttcgtttcg
      181 atattgttga ttgttgatat ttctctcttc aaaatcaatt tgatgctcat taagataatt
      241 ttttacaaat gtacaaggtg ggcaatcatt ctgcgtataa acgattattt ctgacatgta
      301 tcatgctcct tcgtgtttta ttctaatgta atatacacaa ttatacattg aaattcaaat
      361 ttgtgaacac attgtgaact gacataaatt atacacaatt tcatagcgca taatgtttta
      421 aaaatacaca ttatagcaat tataattaag ttcattgtgt tgtcatcaaa aaaagaaaaa
      481 ggtgatgttt ttaaatggat acagttgaaa tcagtcggtt tttgacagct atgactttag
      541 cagttcatat catttttgca acgattggtg ttggtatgcc tttaatgttc gcaattgcag
      601 aatttttagg tattcgcaaa aatgatcttc aatatatagc tatggccaaa agatgggcta
      661 aagcttatac aattactgta gcagtgggag ttgttacagg tacaattata ggacttcaat
      721 tatcattgat ttggcctaca tttatggaaa tgggtggaca cgttattgca cttccattat
      781 ttatggaaac atttgcgttc ttctttgaag ctattttctt aagtatatat ttatatactt
      841 gggatcgttt taaaaataaa tggacacatt tcttaattag tataccagta attattggtg
      901 gctctttctc agcattcttc attacttcag tgaattcatt tatgaatacg cctgcaggtt
      961 ttgagttgaa gaatggaaag atggtcaatg ttcaacctat agaagcgatg tttaacccat
     1021 cgtttatagt tcgatcattt cacgtaatta ctacagccgg tatgacgatg gcatttgtta
     1081 ttgcatcaat agcagctttt aaattattgc gtaatcgtca acctaaagat actgtctacc
     1141 ataagaaagc tttgaaaatg tctatgatag ttggattctt ttcaacatta ctttctatgt
     1201 tggcagggga tttatctgca aaatttttgc ataaattcca acctgaaaaa ttagcagctt
     1261 atgaatggca tttcgataca tcttcccatg ctaaattatt attatttggt gtgttagatg
     1321 aaaagactca gcaagttaaa ggtgcgattg aattacctgg actattgagt ttcttagcag
     1381 ataatagtgt caaaactaaa gtgcaagggt taaatgattt tccaaaaagt ttacatccgc
     1441 ctatgattgt ccattatttc tttgatttaa tggtaacgat gggaatttta tgttttgtca
     1501 tttcaggtgt ttatgtttta actttaatgt ttaaaaagct tagaaagttt tctactcata
     1561 aatggatgct ttacggaata ttattaacag gaccagcttc aatgctagct atagaatttg
     1621 gatggttctt aacagagatg ggtagacagc cttggattgt tcgtggttat atgcgcgtgg
     1681 cagaagcagc aacacaagca ggcggaataa ccttcgttac aattttattt ggcatattgt
     1741 acatcatttt aatgtataca tgtgcatacg tattaattcg tatgtttaaa aataaaccgg
     1801 cgtatgaaga tgtaaatcgt ttagccaaga agcaaggagg agaaatagaa aaatgattta
     1861 tgcatttata ggtataacag tgttatggtt gtttttattt tgctatatca ttattgcttc
     1921 tatagatttt ggggcaggtt tcttcgcatt gcattcaaag ttaactggtg atgaaaagaa
     1981 aattaatcac ttaatttcac gttatttaaa cccagtttgg gaagttacga atgtattctt
     2041 tgttttcttc ttcgtaggat tcgtaggttt ctttccagag tcaatcaaat atttaggcac
     2101 ggtattgtta ataccaggtt caatagcact gattatgata tcgttgagaa acagttttta
     2161 tgcatttgaa aattatggtc aagatacaaa attagcatgg atgatcatgt atggggtaag
     2221 tggattatta attccagctt cattatctac tgctttaact attacagaag gtggctatat
     2281 taatgttcga aacaatgtta tcgatctaga ttgggtgcag ttactattaa gcccatttgc
     2341 ttggtctgta gtattcttgg caattatttc agttttatat atttcatcag gatttttgac
     2401 atattatgct aaaaaagcaa atgacgaacc agcatataat ttaacaagac aatggcacat
     2461 atttttaggg ccgccgatga ttatcatttg tttattcgta tttctatcat tacgcattca
     2521 aaattctgaa catttttatt cagctgtttt tgactattgg tggatgtttg ctataagttt
     2581 cttattcttt gcattagctt cattgttaac attctttaag aaaaaacatg gtttggcttt
     2641 tgtatttgtt attttacaaa tgatgttcgc gttctttggc tatggaatta gtaaattgcc
     2701 atacctttta tatccgtttg taaaaattac agatgcatat gttaatccag aaatgggctg
     2761 gacattagtg attgtcttta ttttaggttt acttttatta cttccatcgt taatattatt
     2821 attaagatta tttgttttcg acaaagaata tgttgaagga aagaaatcat aagttgagac
     2881 gataccccaa ctttcattta cttgcattac tgcatttaga taatttagtt ggggtactca
     2941 tttataataa ttttaaattt atattttcaa tgaattgatg aaaatcattt aatggagggc
     3001 gagtatgggt aaagaatatg tagtcatcgg tctaggtcgt tttggaggta gtatcgttcg
     3061 tgagttgaat gcattagaca tggatgtaat ggccatcgac catgatgaaa atagagtgaa
     3121 tgaatatagt gatatcgcta ctcatgcggt tgttgcagac acaacagatg aagcagttat
     3181 gaaaagttta ggtatccgta actttgatca tgtcattgtg gcaattggtg aaaatattca
     3241 atcaagtacg ttgacgactt taattttaaa agagttaggt gtaaaaaaag taactgctaa
     3301 agcacaaaat gattatcatg caaagatttt aaataaaatt ggagcagata cggttgtgca
     3361 ccctgagcgt gatatgggta gacgtattgc gcataatgtt gcgagtgcaa gtgtacttga
     3421 ttatcttgag ttggcagacg agcattctat tgtagaattg aaagcaactg aaaagatggc
     3481 ggggcagtct atcattgatt tagatataag agcacaatat ggaattaaca ttattgcaat
     3541 taaacgaggc aaagagttca tcatttcacc aaatccaaat attaatttag aaataggtga
     3601 tattttaatc atgattggac atgataatga tttaaatcgc tttgaaaaaa atattgcgac
     3661 gagataatcg ggagtgggac agaaatgata ttttctcaaa atttatttcg tcgtcccacc
     3721 ccaacttaca ttgtctgtag aaattgggaa tccaatttct ctttgttggg gcccacaccc
     3781 caactcgcat tgcctgtaga atttcttttc gaaattctct atgttggggc ccctgactag
     3841 aattgaaaaa agcttgttac aagcgcattt tcgttcagtc aactactgcc aatataactt
     3901 tgtagagcat tgaacattga tttatgtctc aagctcatca tatataaaga aaaagctact
     3961 aacaatgggt ggtgagttta attaaaactt cacttcaaag ttagtagctt tttgattatt
     4021 tattgtttga ttctttttgt tcgtttacct tcataatgac tggtaaaatc attggttttc
     4081 tagctgtttt ttcaaataaa taaggttgta atgtttcaat gatagaagat ttaatctgat
     4141 gccattgaat atctttattt tgatttaact tactaataac atcagttttg attttgcgtt
     4201 gtgcatcata aattaattga cctgattccc tcatatatac aaatcctcga gaaataatgt
     4261 ctggaccaga aagtaattta tttgtattaa aatcaatact aacaacaacg ataactaaac
     4321 cttcttcaga taatagctta cggtctctta ttacaacatt accgatatca ccgataccac
     4381 taccatcaac aagtacatta ccagatggaa tgcgaccagc tttacgtgct gaatcgtgtg
     4441 ttaaagctaa gacatctcca atatcaaaga tgaagacatt atcttcttca acgccgcatt
     4501 caacaccagt ctcaccatgt gcttttaaca tacggtattc accatgaata ggtaagaaat
     4561 atttcggctt gattaatcga agcattaatt gttgatcacc ttgagaacca tgccctgaag
     4621 tatggatgtt agaaatcttg ctatggataa catctgcacc agctttatac aaggaattaa
     4681 tagttctgtt aatacttttt gtattacctg ggataggtga tgaactaaat acaacggtat
     4741 cttcaggtat aattttaatt tgcttatgag taccattagc aattctagat aatgctgcca
     4801 ttggttcacc ttgtgaacca gtacatagta tcaataactc atgcttcggt acggtattaa
     4861 ttttattagg ttcaataaat gtttcaggtg gtgctttaat ataaccaagt tccataccga
     4921 ttttaatatt gttttccatc gaacgaccga acgtaacaat tttacggtta tttttgatag
     4981 cagcttcaac tgcttgttga actcggtaaa tattagaagc gaaggtagca aatataatac
     5041 gacctttaca attacggaag atcttatcta cgttttgacc aacttcacgt tcgcttaaag
     5101 taaaatcagg cacaagtgaa tttgttgagt ctgaaagtaa acatagaacg ccttcttcgc
     5161 ctaattgagc cattttagca atgtttgctg gtttgcctac aggtgtaaaa tcaaatttaa
     5221 agtcaccggt atgaactact tttccttcag gtgtatctac gatgacgcca taagtttcag
     5281 gaatactatg cgtagttaag tagaaagaaa tcgtaaagtg cttagattta atcacactgt
     5341 cctcattgat ttcatttagt ttagcagtac gtaataaatg atgttcttca agtttattac
     5401 ggattaaacc taatgctaaa ggaccaccat aaataggtat attaagttgt tttaatagga
     5461 agggcacacc gcctatatgg tcttcgtgac catgtgttat aaataggcca acaattttat
     5521 cttggttttg aactagatat gtgtagtcag gtataacata atcaatccct aataagttat
     5581 catctgggaa tttgatacct gcatcgataa tgacaatttc gtctttatac tcaactgcat
     5641 aagtattttt accgatttca cctagacctc caagtgcata tacacctact tcatttggat
     5701 gtaattgttt cattatttag caatctccac attaaagtgt tctgagtttt ctttttcgta
     5761 atctaaatgt gcgccctcta atttagtgat aaattcgata ttaaaattac gatctttcaa
     5821 gtaacgacgt acttgttctt ctgtttgagc ttcaacataa agtgattgtg tattttcacg
     5881 cacaattacc tcgtctctgt tatgttgata aaaaacttta aatactgcca tgttaaaaat
     5941 tcctcctaag aatgtttgtt taatttattg ttaaccctag taaaatcgta ttggagtata
     6001 tatcgataaa ttcattccaa tcatctctat aatgtaactt aataacgatt tggttaataa
     6061 ctaggttcat catgtcgttc attttaaaaa attagtgaaa taacactaaa atttcagtta
     6121 aatcttattt tacatgatga atgataataa ataaagcagt ttatctcact agaaatcgaa
     6181 gtatttcgaa taaatgtgca gttctattta ttaaaataaa tgtagagatt ataaattaat
     6241 gttaagaaag aattaaagga ttttatatga aacggtaaaa tttaaatgag gggataaaaa
     6301 gtttggtaga aaggtgaata tatttaactg ttgtttattc gaaaaagttg tacaagaaaa
     6361 agcttaggac ataatattta tcctaagctt tgataattag taaaaatgtg ctttaaactt
     6421 ctacagcatc tgtatgtggt tgtaatgggt gatctttgtc aatgtgatca tagaacatta
     6481 caccatttaa atggtcaatt tcatgttgga aaacaattgc tggatatcct tttagacgta
     6541 attgtatatc attaccttcg atgtctttgg ctttaattgt aattctatta tgacggtgaa
     6601 ctagaccagc aacattatca tcgacactaa ggcaaccttc accagttggt aaataagctt
     6661 cttgaacgct atgacttaca atttttgggt tcacaagcat atagtcataa gatttgccac
     6721 tgccatcatc tggtattaaa acagcaatca tacgtttaga aatattaatt tgaggtgcag
     6781 ccaaaccaac gcctgaacgt aaaccatatc gtttcgcgat ttcctcatct tgactattta
     6841 ctaaaaactc tctcatggcg attaatgttt ctttttcttc tttagttaat ggtaattcta
     6901 actcagctgc tttttgacgc aaagttggat gaccatctct aatgatgtct ttcattgtta
     6961 acatatattg caccttcctt attttaattt gttttagttg aatgacagta aaaaggttgt
     7021 taagatactc atacattttt atgtgtaaat atctacaaag ttaaccaact actgccaatg
     7081 tttattttag atagtatatg taaattttca gatatgctaa ttgcttaaaa aatgattaaa
     7141 gtgttggttt caagcaatga tactttagaa atttatttat catcttgact ttaaaaatta
     7201 tattataaat gacgtaactg tcaacagata tacttagtaa tgaagatgtg taatgtaatt
     7261 gtttaaaatt gatttccaag cagattttat ttatcattta atttaaatag caagtggagg
     7321 tacaagtaat gaaatttgga aaaacaatcg cagtagtatt agcatctagt gtcttgcttg
     7381 caggatgtac tacggataaa aaagaaatta aggcatattt aaagcaagtg gataaaatta
     7441 aagacgatga agaaccaatt aaaactgttg gtaagaaaat tgctgaatta gatgagaaaa
     7501 agaaaaaatt aactgaagat gtcaatagta aagatacagc agttcgcggt aaagcagtaa
     7561 aggatttaat taaaaatgcc gatgatcgtc taaaggaatt tgaaaaagaa gaagacgcaa
     7621 ttaagaagtc tgaacaagac tttaagaaag caaaaagtca cgttgataac attgataatg
     7681 atgttaaacg taaagaagta aaacaattag atgatgtatt aaaagaaaaa tataagttac
     7741 acagtgatta cgcgaaagca tataaaaagg ctgtaaactc agagaaaaca ttatttaaat
     7801 atttaaatca aaatgacgcg acacaacaag gtgttaacga aaaatcaaaa gcaatagaac
     7861 agaactataa aaagttaaaa gaagtatcag ataagtatac aaaagtacta aataaggttg
     7921 gtaaagaaaa gcaagacgtt gatcaattta aataattaat ataatacaga tggtaggaaa
     7981 caactaatac agttcctatt atctgtatct ttttttatta aaacagaact ttttcaaatg
     8041 gtttaacagt cccatttatt tgtggtacaa ttattaagga taaaatgaat ttctatacaa
     8101 ttatgggaaa ggtatggtga attgaatggc tcctaagtta caagcccaat tcgatgcagt
     8161 aaaagtttta aatgatactc aatcgaaatt tgaaatggtt caaattttgg atgagaatgg
     8221 taacgtcgta aatgaagact tagtacctga tcttacggat gaacaattag tggaattaat
     8281 ggaaagaatg gtatggactc gtatccttga tcaacgttct atctcattaa acagacaagg
     8341 acgtttaggt ttctatgcac caactgctgg tcaagaagca tcacaattag cgtcacaata
     8401 cgctttagaa aaagaagatt acattttacc gggatacaga gatgttcctc aaattatttg
     8461 gcatggttta ccattaactg aagctttctt attctcaaga ggtcacttca aaggaaatca
     8521 attccctgaa ggcgttaatg cattaagccc acaaattatt atcggtgcac aatacattca
     8581 agctgctggt gttgcatttg cacttaaaaa acgtggtaaa aatgcagttg caatcactta
     8641 cactggtgac ggtggttctt cacaaggtga tttctacgaa ggtattaact ttgcagcagc
     8701 ttataaagca cctgcaattt tcgttattca aaacaataac tatgcaattt caacaccaag
     8761 aagcaagcaa actgctgctg aaacattagc tcaaaaagca attgctgtag gtattcctgg
     8821 tatccaagtt gatggtatgg atgcgttagc tgtatatcaa gcaactaaag aagcacgtga
     8881 ccgcgcagtt gcaggtgaag gtccaacatt aattgaaact atgacatatc gttatggtcc
     8941 tcatacaatg gctggtgacg atccaactcg ttacagaact tcagacgaag atgctgaatg
     9001 ggagaaaaaa gacccattag tacgtttccg taaattcctt gaaaacaaag gtttatggaa
     9061 tgaagacaaa gaaaatgaag ttattgaacg tgcaaaagct gatattaaag cagcaattaa
     9121 agaggctgat aacactgaaa aacaaactgt tacttctcta atggaaatta tgtatgaaga
     9181 tatgcctcaa aacttagcag aacaatatga aatttacaaa gagaaggagt cgaagtaagc
     9241 catggcacaa atgacaatgg ttcaagcgat taatgatgcg cttaaaactg aacttaaaaa
     9301 tgaccaagat gttttaattt ttggtgaaga cgttggtgtt aacggcggtg ttttccgtgt
     9361 tactgaagga ctacaaaaag aatttggtga agatagagta ttcgatacac ctttagctga
     9421 atcaggtatt ggtggtttag cgatgggtct tgcagttgaa ggattccgtc cggttatgga
     9481 agtacaattc ttaggtttcg tattcgaagt atttgatgcg attgctggac aaattgcacg
     9541 tactcgtttc cgttcaggcg gtactaaaac tgcacctgta acaattcgta gcccatttgg
     9601 tggtggcgta cacacaccag aattacacgc agataactta gaaggtattt tagctcaatc
     9661 tccaggtcta aaggttgtta ttccttcagg cccatacgat gcgaaaggtt tattaatttc
     9721 ttctattaga agtaatgacc cagtcgtata cttagagcat atgaaattgt atcgttcatt
     9781 ccgtgaagaa gtacctgaag aagaatatac aattgacatt ggtaaggcta atgtgaaaaa
     9841 agaaggtaat gacatttcaa tcatcacata cggtgcaatg gttcaagaat caatgaaagc
     9901 tgcagaagaa cttgaaaaag atggttattc tgttgaagta attgacttac gtactgttca
     9961 accaatcgat gttgacacaa ttgtagcttc agttgaaaaa actggtcgtg cagttgtagt
    10021 tcaagaagca caacgtcaag ctggtgttgg tgcagcagtt gtagctgaat taagtgaacg
    10081 tgcaatcctt tcattagaag cacctattgg aagagttgca gcagcagata caatttatcc
    10141 attcactcaa gctgaaaatg tttggttacc aaacaaaaat gacatcatcg aaaaagcaaa
    10201 agaaacttta gaattttaat acattttaaa agttaacgaa gttagcgtat tttagtctca
    10261 ttgattaaaa tgaaatgttt aatttacgaa atcttaggag ggcaaaaacg tggcatttga
    10321 atttagatta cccgatatcg gggaaggtat ccacgaaggt gaaattgtaa aatggtttgt
    10381 taaagctgga gatactattg aagaagacga tgttttagct gaggtacaaa acgataaatc
    10441 agtagtagaa atcccatcac cagtatctgg tactgtagaa gaagttatgg tagaagaagg
    10501 tacagtagct gtagttggtg acgttattgt taaaatcgat gcacctgatg cagaagatat
    10561 gcaatttaaa ggtcatgatg atgattcatc atctaaagaa gaacctgcga aagaggaagc
    10621 gccagcagag caagcacctg tagctactca aactgaagaa gtagatgaaa acagaactgt
    10681 taaagcaatg ccttcagtac gtaaatacgc acgtgaaaaa ggtgttaaca ttaaagcagt
    10741 ttctggatct ggtaaaaatg gtcgtattac aaaagaagat gtagatgcat acttaaatgg
    10801 tggtgcacca acagcttcaa atgaatcagc tgcttcagct acaagtgaag aagttgctga
    10861 aactcctgca gcacctgcag cagtaacatt agaaggcgac ttcccagaaa caactgaaaa
    10921 aatccctgct atgcgtagag caattgcgaa agcaatggtt aactctaagc atactgcacc
    10981 tcatgtaaca ttaatggatg aaattgatgt tcaagcatta tgggatcacc gtaagaaatt
    11041 taaagaaatc gcagctgaac aaggtactaa gttaacattc ttaccttatg ttgttaaagc
    11101 acttgtttct gcattgaaaa aatacccagc acttaacact tcattcaatg aagaagctgg
    11161 tgaaatcgtt cataaacatt actggaatat cggtattgca gcagacactg atagaggatt
    11221 attagtacct gttgttaaac atgctgatcg taagtctatt ttccaaattt cagatgaaat
    11281 taatgaatta gctgttaaag cacgtgatgg taaattaaca gccgatgaaa tgaaaggtgc
    11341 tacatgcaca atcagtaata tcggttcagc tggtggacaa tggttcactc cagttatcaa
    11401 tcacccagaa gtagcaatct taggaattgg ccgtattgct caaaaaccta tcgttaaaga
    11461 tggagaaatt gttgcagcac cagtattagc attatcatta agctttgacc acagacaaat
    11521 tgatggtgca actggccaaa atgcaatgaa tcacattaaa cgtttattaa ataatccaga
    11581 attattatta atggaggggt aaaacatggt agttggagat ttcccaattg aaacagatac
    11641 tatagtaatc ggagcaggtc ctggtggata cgttgcagca attcgtgcag ctcaattagg
    11701 acaaaaagta acaatcgttg agaaaggtaa tcttggtggt gtttgcttaa acgtaggatg
    11761 tattccttca aaagcattac tacatgcttc tcaccgtttt gttgaagcac aacattctga
    11821 aaacttaggt gttattgctg aaagtgtttc tttaaacttc caaaaagttc aagaattcaa
    11881 atcatcagtt gttaataaat taactggtgg tgttgaaggc ttacttaaag gtaacaaagt
    11941 taacatcgtt aaaggtgaag catatttcgt agataacaat agcttacgtg ttatggacga
    12001 aaagagcgca caaacataca actttaaaaa tgcaatcatt gcaacaggtt caagaccaat
    12061 tgaaattcct aatttcaaat tcggtaaacg tgttatcgac tcaacaggtg ctttaaactt
    12121 acaagaagta ccaggtaaat tagttgtagt tggtggagga tacattggat cagaattagg
    12181 tacagcattt gctaactttg gttcagaagt aaccatcctt gaaggtgcta aagatatctt
    12241 aggtggcttc gaaaaacaaa tgacacaacc tgttaaaaaa ggtatgaaag aaaaaggtgt
    12301 tgaaatcgtt actgaagcta tggctaaatc agctgaagaa acagataacg gagttaaagt
    12361 tacttatgaa gctaaaggcg aagagaaaac aatcgaagct gattatgtat tagtaactgt
    12421 aggtcgtcgt ccaaacacag acgaattagg cctagaagaa ttaggtgtta aattcgctga
    12481 ccgtggatta ttagaagttg ataaacaaag ccgtacgtct atcagcaata tctatgcaat
    12541 tggtgatatc gttccaggtt taccacttgc tcacaaagct agctatgaag ctaaagttgc
    12601 tgctgaagca attgatggtc aagctgctga agttgattac attggtatgc cagcagtatg
    12661 ctttactgaa ccagaattag ctacagttgg ttattcagaa gcgcaagcta aagaagaagg
    12721 tttagcaatt aaagcttcta aattcccata tgcagcaaat ggtcgtgcat tatcattaga
    12781 tgatactaac ggatttgtta aacttattac acttaaagaa gatgatactt taatcggtgc
    12841 tcaagtagtt ggtactggtg catcagatat tatctctgaa ttaggtttag caattgaagc
    12901 tggtatgaat gctgaagata tcgcattaac aatccatgca catccaacat taggtgagat
    12961 gactatggaa gcagcagaaa aagctatcgg atacccaatc catacaatgt aataactgat
    13021 tatctataaa gattcagtca ttaaaagctg tagcatatgc tacggctttt ttgttttagg
    13081 taaagtaatg taaggaaatt gatttgagat atcgttaaca tgtgacatgc atgttatact
    13141 agcgatgcta acaaaagaat tgaaatggag ggttcaacaa tggaatatga gtatccaatt
    13201 gatttagact ggagtaatga agagatgatt tcagtgataa atttctttaa tcatgtagag
    13261 aagtattatg aatccggcgt gacggcaggc gactttatgg gtgcatataa aagatttaaa
    13321 gaaattgtgc ctgctaaagc agaggaaaaa caaattttta atactttcga aaaaagtagt
    13381 ggctataata gttacaaagc agttcaagat gtaaaaactc actctgaaga acaaagagta
    13441 acagctaaaa aataattcgt tcgaaattaa tacaatttaa taggaatttt tctttaaaac
    13501 tattgct
//
LOCUS       DAMZLE010000063        13330 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMZLE010000063 DAMZLE010000000
VERSION     DAMZLE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.063"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(153..959)
                     /gene="yjbH"
                     /locus_tag="POB92_001597"
     CDS             complement(153..959)
                     /gene="yjbH"
                     /locus_tag="POB92_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDI6579099.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(982..1347)
                     /locus_tag="POB92_001598"
     CDS             complement(982..1347)
                     /locus_tag="POB92_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDI6579100.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1451..2044)
                     /locus_tag="POB92_001599"
     CDS             complement(1451..2044)
                     /locus_tag="POB92_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDI6579101.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQINYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            2230..2577
                     /locus_tag="POB92_001600"
     CDS             2230..2577
                     /locus_tag="POB92_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579102.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            2594..3229
                     /locus_tag="POB92_001601"
     CDS             2594..3229
                     /locus_tag="POB92_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI6579103.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            3246..4055
                     /locus_tag="POB92_001602"
     CDS             3246..4055
                     /locus_tag="POB92_001602"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDI6579104.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            4052..4906
                     /locus_tag="POB92_001603"
     CDS             4052..4906
                     /locus_tag="POB92_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6579105.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQENGENINPHIVTRLDRNTTGIVIFAKYGYIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDRIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            4927..6312
                     /gene="mgtE"
                     /locus_tag="POB92_001604"
     CDS             4927..6312
                     /gene="mgtE"
                     /locus_tag="POB92_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDI6579106.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            6322..8166
                     /locus_tag="POB92_001605"
     CDS             6322..8166
                     /locus_tag="POB92_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDI6579107.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNNDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIVSKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            8444..9214
                     /gene="fabI"
                     /locus_tag="POB92_001606"
     CDS             8444..9214
                     /gene="fabI"
                     /locus_tag="POB92_001606"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI6579108.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(9409..10494)
                     /locus_tag="POB92_001607"
     CDS             complement(9409..10494)
                     /locus_tag="POB92_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6579109.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNSLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            10836..12404
                     /locus_tag="POB92_001608"
     CDS             10836..12404
                     /locus_tag="POB92_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDI6579110.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            12549..13307
                     /locus_tag="POB92_001609"
     CDS             12549..13307
                     /locus_tag="POB92_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6579111.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTISIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFLRKNTDDQHYE"
BASE COUNT         4495 a         1856 c         2400 g         4579 t
ORIGIN      
        1 ttgtataatt ttcgaactgg ttaaattcgg aaaatatcac taatatattt agatcgatgt
       61 gtaacattac gttctaataa tttaatgttg caaaattaag agtcagtaaa aagacctcgt
      121 tacatttatg gtgtacgagg tctttaattt atttattttg atttgatttt aggcatttta
      181 gatttccaga aatcgccatc tggatattta agtttttcaa ttttttgttg aatggctaac
      241 ttctttaact ctttgtttaa aagtttttct ggccattcat aaatagtaag taattcttcc
      301 atcgttacaa gttgttgttg ctgtatataa gtttctaatt taggaggaag attcttttcg
      361 ataggtttac ccatcaattc attaattata taagtataga tgtgatatgg gtataatcct
      421 tcgactttta aaccttcttc atgaacatct tcactaaaga aaacgagaga aggggcttgt
      481 tcgatttcca tttctcttgc aatatgcaaa tcaattttca agctttcggt tagtttactt
      541 ttttgtaagt cgtctttaaa tacttctaaa tcaatacctg cattttgaat acagtcacaa
      601 atcattgatt ctgtaataat atctctttta ggtatgattt cattttgcat taaatgtata
      661 aatcgttcgg cacgtacacg accttgtaac tcagctgctt tataagctag ggcgatgtta
      721 tcaaagttgg atgtactttg agcttggcat ttcgttaata cttttaacga aggatttaat
      781 atatgtctga tacgtatata ttgattatat tcaattctta atttggataa gattgctgat
      841 aatttgaagc aatcggagct aaatggatcg aaaaatgaat aaatttcgat tttacttaca
      901 ggtgatagat taatatcttc acgactctta ttttccatta ttcgtaattc tccagccatg
      961 tttattcacc tacaattaat tttaggaatt caccatatga ttagcagtta atcttaagcg
     1021 ctcaaataaa taatctccaa caccttgtgg aaacgcagcg cgattaattg ctgtctgcat
     1081 attttctaac catgcatctc tttcaaattc agtgattgta aaatccatat gtctttttct
     1141 tagcatagga tgtccgtgtt cttcggtata aatgtttgga ccgcccaaaa actgtgttaa
     1201 aaattgtttt tgtttacgac ttgtttctgc aaaatctcct ggaaacaggt gattaagtcg
     1261 ttcatctttt tctacaaggg tgtaaaaata atcaatcata tcgtataacg cttctttacc
     1321 aatgatgtca tatggtgttg ttgtcattta atcacccatt ttcaaaaatt tactgttacg
     1381 aacttaagtt aatatataac taatataaca tgattttaaa catttgaaag aaatatgcat
     1441 atttgccaat ttaatttata ttgtttgaaa gtgtttcttt tttcttgaaa aaacgttgaa
     1501 ctttatttaa aggttgatga tgttcgaggt ttagttcgtt taataaagat tggaactttt
     1561 gtaaaccttg attatagtct ttaacttcga actctaactc ataatccgta gtatcgaaat
     1621 actcactttt atctaaaacg agtaaatcac ctttatattt agtttcttgg cgatatgtcg
     1681 ttaatgcacc aagtattgat aaagttgtat cctttacacc aaactgttca actataattt
     1741 gacgaatgtc atctggaaga ttgtcgtttg aaataatcaa gttcatctct ggtttaatgt
     1801 cgacgatata gttgtattct aatagaccaa cctttgctgg tgtctttaaa gtcatttcat
     1861 attgattgtc tttaactctt atgcgtagtg cagagcgatg ttcctttaat ttgaaatcgg
     1921 gtgtatcaat atagtaattg atttgcttaa aaagcacact gtctttaaaa tatttctctt
     1981 gcaatttatt atagattgat gcagttatca tttgtttaaa ttctatttca tgatttgttg
     2041 ccatgatatg tatacacctc gtatcaaatt caatttatct taactatatt atgaatgaca
     2101 aagttgaatt ttaaaagcaa tttcctttat ctattatcaa tgttaatttg accattaaaa
     2161 atagtgttcg taagtgtttt gtattattga attgtgttaa aatgttatgg aataagagga
     2221 ggattaagca tgcgtttata tattaatgaa attaaaatta aagatgacat actttattgt
     2281 tatacagaag attctattaa aggattatct gaagtaggac aaatgctcgt tgatagtgat
     2341 aattatgcct ttgcgtatac attagatgat ggtaaagcgt atgcttatct cattttcgta
     2401 caagaaacat ggacgatgtt gcatgaaaac atgactaaaa aaattattat caatgatgaa
     2461 ctagaattga ctgaattcca ccaagaactt acttatattt tagacaacat aaaagggaat
     2521 aataattatg gtaaggaatt tgttgcaacc gttgaagaaa cattcgacat tgaataaagc
     2581 ggggtgaagc actatgaatc aatgggatca gttcttaaca ccttataagc aagcggttga
     2641 tgagttgaaa gtgaaactta aaggcatgcg caaacaatat gaagttggtg aacaagcgtc
     2701 gccaatagaa tttgttactg gtcgtgttaa accaatcgct agtattatag ataaggcaaa
     2761 caaacgacaa ataccatttg ataggttaag agaagaaatg tacgatatcg ctggtttaag
     2821 aatgatgtgc caatttgttg aagatattga tgttgtcgtc aatattttaa gacaaagaaa
     2881 agattttaaa gtaattgaag aacgagatta tattcgtaac actaaagaaa gtggttaccg
     2941 ctcgtatcat gtcattattg aatatccaat tgaaacatta caaggccaaa aatttatatt
     3001 ggctgagatt cagattcgta cattagcaat gaatttctgg gcaacgattg aacatacttt
     3061 acgatataaa tatgatggtg cttatccgga tgaaattcaa catcgtttgg aaagagcggc
     3121 agaagcagcg tatttacttg atgaagagat gtctgaaatt aaagatgaaa ttcaggaagc
     3181 tcaaaaatat tacacgcaaa aacgttctaa aaaacatgaa aatgattaac gaggtgttat
     3241 aaatcatgcg ttatacaatt ttaactaaag gtgactccaa gtctaatgcc ttaaagcata
     3301 aaatgatgaa ctatatgaaa gattttcgca tgattgagga tagtgaaaat cctgaaattg
     3361 ttatttcagt tggtggtgat ggtacattac tacaagcatt ccatcagtat agccacatgt
     3421 tatcaaaagt ggcatttgtt ggagttcata caggtcattt aggattttat gcggattggt
     3481 tacctcatga agttgaaaaa ttaatcatcg aaattaataa ttcagagttt caggtcattg
     3541 aatatccatt gcttgaaatt attatgagat acaacgacaa cggctatgaa acaaggtatt
     3601 tagcattaaa tgaagcaacg atgaaaactg aaaatggctc aacacttgtt gtggatgtta
     3661 acttaagagg gaaacacttt gagcgattta gaggcgatgg attatgtgta tcaacacctt
     3721 cgggttcaac ggcttataac aaagcgctag gtggcgcact gatacatcct tcacttgaag
     3781 caatgcaaat tacagaaatt gcctcaataa ataatcgtgt gtttagaacg gtaggatcac
     3841 cacttgtatt accaaagcat catacatgtt taatatcacc agttaatcat gataccatta
     3901 gaatgacgat agatcatgtt agtatcaaac ataaaaatgt taattcaata caataccgtg
     3961 tagcaaatga aaaagtgagg tttgcacgtt ttagaccatt cccattctgg aaacgtgtac
     4021 acgattcttt catatcaagt gatgaagaac gatgaaattt aagtatcata tatcacaaca
     4081 ggaaactgtt aaaacttttt tagcacgaca tgatttttct aagaagacag tgagcgccat
     4141 taaaaataat ggcgctttaa ttgttaatga tgaaccagtc acagtgcgta agcaattaat
     4201 gccaaatgat atattagaaa ttcatttacc acgagaaata ccgagtgtta atttaatacc
     4261 ttatgctcgt aagctagaag tattgtatga agatgctttt atcatcatag ttactaaacc
     4321 aaacaatcaa aattgtacac cttcgagaga acatcctcat gaaagtttaa tcgaacaagt
     4381 actatatcat tgtcaggaaa atggtgaaaa tattaaccca catattgtta cgcgtctaga
     4441 tcgtaataca actggtattg tgatattcgc taaatatgga tatatccatc atttattttc
     4501 taaagtaaac ttgaaaaaaa tatatacttg ccttgtatat ggtaaaaccc atacatctgg
     4561 tattattgaa gctaatatta gacggtcaaa ggatagtatt ataactagag aagttgcctc
     4621 ggatggtaaa tacgctaaaa catcttatga agtaataaat cagaatgata aatacagttt
     4681 atgcaaagtt catttgcata cgggacgtac acatcaaatt cgtgtacatt ttcaacatat
     4741 tgggcatcca attgtgggag attctttgta tgatggtttt catgacagaa ttcatggtca
     4801 agtactgcaa tgtacgcaaa tatattttgt tcatccaatc aataagaaca atatttatat
     4861 tacaattgat tataagcaat tacttaaatt attcaatcaa ctctaattca cacagggggt
     4921 gtaagtatgt caatgaacac agatgaaaaa gagcgtgttc aagaggaatt atatgatgag
     4981 acattattag atcaatactt agaaaatgat gatattgatc aatttagaga tgaatttcta
     5041 gcattacaca catatgaaca aagtgagtat tttgaagata ctaccgatga aaatagacaa
     5101 aagatttttc aatatttatc acctgaagag gttgcaaatt tctttgatca attagatatt
     5161 gatgacgatg aatatgagtt gctatttgat aaaatgaatg cgacatacgc aagtcacata
     5221 ttagaagaaa tgtcatacga taatgcagta gatattttaa atgagttgac taaaccaaaa
     5281 gttgctagtc ttttaacatt gatgaataaa gatgacgcga atgaaatcaa agcattactt
     5341 cactatgatg aggatacggc cggcggtatt atgacgacgg agtatttatc acttaaagcg
     5401 catacgcctg ttaaagaagc attattattg gtcaaagcgc aagcaccaga cgcagaaaca
     5461 atatatgtta tatttgtcgt tgatgatgat ggtaaattag taggtgtttt atcgctaaga
     5521 gatttaattg tagctgaaaa tgatgcttat attgaagata ttatgaatga acgtgtcatt
     5581 agtgtgaatg tagcagacga ccaagaagat gttgctcaag ttatgagaga ctatgatttc
     5641 atggctgtac ctgttataga ttaccaagaa catttgcttg gtatcatcac gattgatgat
     5701 attttagacg ttatggatga agaggctagt gaagactact ctcgtttagc cggggtatca
     5761 gatatcgatt cgactaatga ttcaatcatt aaaacagcat taaaacgttt accatggttg
     5821 attattttaa catttttagg aatgattact gcgacaattt tagggagatt cgaaaaaaca
     5881 ttagaaaatg tagcgctact cgcagcgttt attcctatta ttagtggtat gtcaggaaat
     5941 tcaggtacac aatctttagc cgtttcagtt cgtaacatta cgacagggga aattaatgag
     6001 caaagtaaat ttagaattgc attaagagaa gcaggaagtg gtgtattatc gggtgttgta
     6061 tgttcaacaa tattatttac aattattgtt gcaatatatc atcagccact tttagcatta
     6121 atcgttgcag gaagtttaac ttgtgcgatg acggtgggga cgtttgtagg ttcgatgatt
     6181 ccattattga tgaataaatt aaatatcgat ccagcagtgg ctagtggacc atttattaca
     6241 acaattaatg atattattag tatgttgatt tattttggtt tagctacatc atttatggct
     6301 tacttaattt aaggaggagt tatggagttt ttatctttag ttattgttgt tttagcagcg
     6361 tttttaactc caataattgt caatcgatta aatattaatt tcttgccagt tgttgttgca
     6421 gaaattttga tggggattgt gattggaaat tcatttctaa atatagtaga aagggattca
     6481 attctaaata ttttatcaac attaggcttt atctttttaa tgtttttaag tggtttagaa
     6541 attgatttta aagcttttaa aaaagataaa cgcgcacgtc aaggacaaaa taatgatgaa
     6601 tcctcaattc cagggcatct taatctagcg ttaactgtat ttgcatttat tatgattatt
     6661 tcgattcttt tagcgtatgt atttaaatgg cttggattag tggatgatgt gttattaatg
     6721 gtcattatca tttcaactat ttccttaggc gtagttgttc caactttaaa agaaatgaat
     6781 attatgagaa caactatagg gcaatttatc ctattagtag cagtacttgc ggacttagta
     6841 actatgattt tattaacggt ctatggtgca atcaatggtc aaggcggcag tacaatatgg
     6901 ttaataggta tattagttgt tttcacagca atttcatata ttttaggtgt tcaatttaaa
     6961 agaatgtcat ttttacaaaa attgatggat ggtacgacgc aaatcggtat tcgtgcggta
     7021 tttgcattaa taatattatt agtagcccta gcagagggag ttggcgcaga aaatatatta
     7081 ggtgcattct tagcaggtgt cgttgtttca ttattaaatc cagatgaaga aatggttgaa
     7141 aagttagact catttggtta tgggttcttt attcctattt tctttataat ggttggtgta
     7201 gatttaaaca taccttcatt aattaaagaa ccgaaattac taattatcat accgatttta
     7261 atcgttgcat ttatcgtttc aaaattaatt ccagtcatgt ttattcgacg ttggtttgat
     7321 atgaaaacaa cgattgcatc agcattttta ttaacatcaa cattatcgct cgtgatagct
     7381 gcagccaaaa tttcagaaag attaaatgct atttcagctg aaacgtcagg tatattaatt
     7441 ttaagcgcag tcattacatg tgtattcgtt ccgattattt tcaaaaaact gtttccagtt
     7501 ccagatgagt ttaaccgtaa aattgaagtt agtttaattg gtaaaaatca attaacgatt
     7561 cctatagcgc aaaatttaac atctcagtta tatgacgtga cattatatta tcgcaaagac
     7621 ttgagtgatc gtcgtcaatt gtcggatgat atcacgatga tagaaattgc tgattatgaa
     7681 caagatgttt tagaacgact aggtctgttt gaccgagaca tagttgtttg tgccacgaat
     7741 gacgatgata ttaaccgaaa agttgctaaa ttagccaaag cacatcaagt tgagcgtgtc
     7801 atttgcagac ttgaaagcac aacggacgat acagagttag ttgattcagg tattgaaatt
     7861 ttcagtagct acttaagtaa taaaatctta ttaaaaggtt taattgaaac acctaacatg
     7921 ttgaatttat taagtaatgt tgaaacgtca ctatatgaaa ttcaaatgtt aaattataaa
     7981 tatgaaaata ttcaattacg taatttccca ttcggaggag acatcatctt cgtgcgtatt
     8041 atccgtaata atgagtcgat tgttccgcat ggagatacac aattgcgata tggagatcgc
     8101 ttaattgtta ccggtgctaa agaatacgtt gatgaattga agcaagagtt agaattttat
     8161 ttttaacaat aatgatatta aaatgcagac gtattttagt acgacgtaaa attaatgata
     8221 ttaaaatgct agtatgtata tgattttgat aaataaatgc tttttacgta aatcaagttt
     8281 gatacagaaa ggactaaatc aaacatttat cgttgtaata cgtttaaata actttattaa
     8341 aaagtcataa tagtgttaaa atgtattgac gaataaaaag ttagttaaaa ctgggattag
     8401 atattctatc cgttaaatta attattataa ggagttatct tacatgttaa atcttgaaaa
     8461 caaaacatat gtcattatgg gaatcgctaa taagcgtagt attgcttttg gtgtcgctaa
     8521 agttttagat caattaggtg ctaaattagt atttacttac cgtaaagaac gtagccgtaa
     8581 agagcttgaa aaattattag aacaattaaa tcaaccagaa gcgcacttat atcaaattga
     8641 tgttcaaagc gatgaagagg ttattaatgg ttttgagcaa attggtaaag atgttggcaa
     8701 tattgatggt gtatatcatt caatcgcatt tgctaatatg gaagacttac gcggacgctt
     8761 ttctgaaact tcacgtgaag gtttcttgtt agctcaagac attagttctt actcactaac
     8821 tatcgttgct catgaagcta aaaaattaat gccagaaggt ggtagcattg ttgcaacaac
     8881 atatttaggt ggcgaattcg cagttcaaaa ctataatgtg atgggtgttg ctaaagcgag
     8941 cttagaagca aatgttaaat atttagcatt agacttaggt ccagataata ttcgcgttaa
     9001 tgcaatttca gctggtccaa tccgtacatt aagtgcaaaa ggtgtgggtg gtttcaatac
     9061 aattcttaaa gaaatcgaag agcgtgcacc tttaaaacgt aatgttgatc aagtagaagt
     9121 aggtaaaact gcggcttact tattaagtga tttatcaagt ggcgttacag gtgaaaatat
     9181 tcatgtagat agcggattcc acgcaattaa ataatatcat tcaacagctt tgttcacgtt
     9241 attatatatg tgagcaaagc ttttttgctt ttataataat cggactgatg gaaaattatt
     9301 tgatatttca tttgactgat tttttatgca caaaaaaatc gcctcaaaag ctgtagtttc
     9361 aacttttgat gcgatagtat cttatattct taaaagacat ccacagaatt agtccttaac
     9421 attactgttt gctttatcaa taatgcgttg gcggtatttg aaaatattac taacaaccgt
     9481 tttaagtaca gcatataatg gcactgcaat cagaactaat gtaaagccac ctaaatctcc
     9541 tgctgctaaa ataacaacga taattgttag aggatggata cttaaagatt tacccattac
     9601 atttggcgta atgatattac cttcaagttg gtgtgcaatt aatgtaatga tacaaaccca
     9661 tataaatgta gtaggactat ctataatacc gagtattgct gcaggtgcaa atgataacca
     9721 tgaacctaag aaaggaatta agtttgcgac accagcaaat agtactaata aaggaatata
     9781 tggtaagtca ataattgaat aaccgatata taagaatata cctaaaataa cactgacagt
     9841 tacttgacct tgaatgtaag attttaatgt aaagtttaaa tcagttaata aatctacgaa
     9901 aaatacttta cgttcacctt tgaaaaattt agcaacagct gggataaatt tttcatggtc
     9961 ttttaacata taaattaaga agaatggaac cataatcaat aagaagatgg ttgaaattaa
    10021 tgatgtaatg tactgtaatg aattagataa aatattagta acgccatcac ccattgattt
    10081 aaccatattt gtaattctac ttgttacatc ttcaggtaat ttatccattt gagctagtgc
    10141 gaatttaata atttgctctg cttctttttg tagggcaggt gtctgactaa ttaaattgtt
    10201 gatatttgaa atgatgattg gtgcaacaaa cgcaacaatt aagccgataa tagcaaacaa
    10261 agctaacatg attgttgtta tactagccca tcttggaaaa ccgacttttt caagtgagtt
    10321 ttgaaaaggt agacagatat aaaataaaaa accactaatt aaaaatggaa gaaatacaga
    10381 accgatgatt gtagctattg gagtaaatac ttcgtgcact tccataaata gtttgatgag
    10441 aatgaacagc ataatcagag cgatgccagt tcggaaccaa accttgttta acataaacga
    10501 tgttcctcct atttttctaa acttagttat tatttagtat atcctaaata gcagtgctta
    10561 aaaaggttaa tattttttaa atatacttaa attaaccatt cactttctat tttaagatat
    10621 ttactttcaa tagtttgtga gacttttcaa tgactcgtaa gtctgcaatt ggatcaaaca
    10681 gcaccagttt atatgaataa cttattttaa tattaaacgt gtttaaataa tgaaataatc
    10741 atattaatat ttataagaat attctgaaaa atatattgaa gtgtctcgtg cttaatcgta
    10801 taatattttt atgtttaaac aataggggga atcttatgat tgaaaaatta gtaacctttt
    10861 taaatgaggt tgtatggagt aagccattag tttatggttt gctaattact ggtgtgctat
    10921 ttacattgcg tatgcgattt tttcaagtta gacattttaa agaaatgatt cgattaatgt
    10981 ttcaaggaga gaagtctcct aatggtattt caagttttca agcgatagcc atgtctttag
    11041 caggcagggt tggtacaggt aatattgtcg gtgtatctac tgcaatattt ataggaggac
    11101 ctggtgcagt attttggatg tggattactg cgtttttagg tgcaagtagt gcttttattg
    11161 aatctacact tggtcaaata ttcaagagag ttgaaaataa tgaataccgt ggtggaccag
    11221 cgtattatat tgaatatggt attggtggta aatttggtaa aatttacgga attatctttg
    11281 ctattgttac gattatctca gtaggtctat tgcttcctgg tgtgcaatct aacgctatag
    11341 caagttctat gcataatgcg attcatgttc cacaatggtt aatgggtgct attgttgtag
    11401 ttattttggg attaattatt tttggtggtg tacgtagtat tgccaatgtt gcaacagccg
    11461 ttgtaccatt tatggcaatt atttacatac tgatggctgt cattatcatt tgtatcaata
    11521 tacaagaagt gccagcgtta tttgcattaa ttttcaaatc agcatttgga ttacaatctg
    11581 cttttggtgg tatcgttggc gcaatgatag agattggtgt taaacgtgga ttatactcaa
    11641 atgaggctgg tcaaggtaca ggtccacacg cagcagcggc agcagaagta tcacatccaa
    11701 gtaaacaagg tctagtacaa gcattttcag tttatattga tacattattt gtatgtactg
    11761 caactgctct gattatactt atttctggta catataatgt gactgatggt acggttaatg
    11821 cgaatggcac accgcattta attaaagatg gcggtattta tgttgaaaat gcaacaggta
    11881 aagattattc aggtactgcg atgtatgcac aagccggcat tgataaagcg ttccatggca
    11941 gtggttatca atttgatcct actttctctg gcgtaggttc gtactttatt gcatttgctt
    12001 tattcttctt tgcatttact acaattttgt cgtactacta cattacagaa acaaatgttg
    12061 cttatttaac gcgtaatcaa aataatcaag tttcatcgat atttattaat attgctcgtg
    12121 tgattatttt gttcgctaca ttttacggtg cagttaaaac agctgatgta gcatgggcat
    12181 tcggtgattt aggtgtaggt ctaatggctt ggttaaatat cattgcgatt tggattttac
    12241 ataagcctgc cgtaaatgct ttaaaagatt atgaaattca aaagaaacgt ttaggaaacg
    12301 gttataatgc agtttatcaa cctgatccga ataaattacc taatgctgtc ttttggttga
    12361 agacatatcc agaacgttta aaacaagcac gtgccaaaaa gtaatctact tttgtttata
    12421 gtatatgtag tgatcatttg ataaaaaaag aaacagcatt gataatttta ggtgctcaga
    12481 aatttgaatt ttaaaaatat agtgtctctt ggtacaataa caatacaact actagggggc
    12541 acttttttat gtcagaattt aaaactggta agattaataa acatgtttta tatagtaata
    12601 ttttaaatag agatgtcacg ataagtattt atttaccaga atcttataat caacttgtta
    12661 aatataatgt cattctttgc tttgacggat tagatttttt acgtttcggg agaatacaac
    12721 gtacatatga atcgttaatc aaagaagcgc gtattgatga tgcgatcatt gttggattcc
    12781 attatgaaga cgttgataag cgtagagagg aatttcatcc acaaggaagt cgttctcatt
    12841 taactattca atcagtcggt aaagaaatat tgccatttat tgactcgacg ttttctacac
    12901 tgaaagtagg taatgcaagg ttattagtag gggatagttt agcgggtagt attgccttat
    12961 taacggcgtt gacctatcca acgattttta gtcgtgtagc aatgttaagt ccacattcag
    13021 atgacaaagt attagataag ctaaatcaat gtgctaataa agaacaattg acaatttggc
    13081 atgtcattgg tctagatgaa aaagatttta ctttaccaac aaatggtaag cgtgccgatt
    13141 tcttaacacc gaatagagaa ttagctgaac aaattaagaa atataatata acttattatt
    13201 acgatgaatt tgaaggtggt caccaatgga aagattggaa accattgctg tcagatatat
    13261 tattgtattt tttacgtaaa aacacagatg atcaacatta tgagtaattt acattagtag
    13321 atttagtatg
//
LOCUS       DAMZLE010000064        13226 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMZLE010000064 DAMZLE010000000
VERSION     DAMZLE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.064"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(129..659)
                     /locus_tag="POB92_001610"
     CDS             complement(129..659)
                     /locus_tag="POB92_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6579112.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(748..2004)
                     /locus_tag="POB92_001611"
     CDS             complement(748..2004)
                     /locus_tag="POB92_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6579113.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKS
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(2007..2207)
                     /locus_tag="POB92_001612"
     CDS             complement(2007..2207)
                     /locus_tag="POB92_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDI6579114.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            2354..2818
                     /locus_tag="POB92_001613"
     CDS             2354..2818
                     /locus_tag="POB92_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDI6579115.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            2825..3100
                     /locus_tag="POB92_001614"
     CDS             2825..3100
                     /locus_tag="POB92_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6579116.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            3378..4589
                     /locus_tag="POB92_001615"
     CDS             3378..4589
                     /locus_tag="POB92_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDI6579117.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKNKSNPKIDVDRTYIEPQQFQS
                     KKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMFPM
                     LLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILAIW
                     SASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVISH
                     FLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFTSI
                     IWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQRSV
                     IKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDKNI
                     KDKIVDKLKKD"
     gene            complement(4701..5330)
                     /gene="vraR"
                     /locus_tag="POB92_001616"
     CDS             complement(4701..5330)
                     /gene="vraR"
                     /locus_tag="POB92_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDI6579118.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(5320..6363)
                     /locus_tag="POB92_001617"
     CDS             complement(5320..6363)
                     /locus_tag="POB92_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6579119.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(6360..7061)
                     /locus_tag="POB92_001618"
     CDS             complement(6360..7061)
                     /locus_tag="POB92_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDI6579120.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(7076..7462)
                     /locus_tag="POB92_001619"
     CDS             complement(7076..7462)
                     /locus_tag="POB92_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579121.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(7679..8437)
                     /gene="map"
                     /locus_tag="POB92_001620"
     CDS             complement(7679..8437)
                     /gene="map"
                     /locus_tag="POB92_001620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI6579122.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            8968..9954
                     /locus_tag="POB92_001621"
     CDS             8968..9954
                     /locus_tag="POB92_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6579123.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            complement(10385..10798)
                     /locus_tag="POB92_001622"
     CDS             complement(10385..10798)
                     /locus_tag="POB92_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579124.1"
                     /translation="MKPTKLILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(10827..10938)
                     /locus_tag="POB92_001623"
                     /pseudo
     CDS             complement(10827..10938)
                     /locus_tag="POB92_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11024..11755)
                     /locus_tag="POB92_001624"
     CDS             complement(11024..11755)
                     /locus_tag="POB92_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6579125.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(11757..13070)
                     /gene="murT"
                     /locus_tag="POB92_001625"
     CDS             complement(11757..13070)
                     /gene="murT"
                     /locus_tag="POB92_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDI6579126.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
BASE COUNT         4207 a         2367 c         1900 g         4752 t
ORIGIN      
        1 aatagtctat aataaaaggg ttaaatttta actaaaagca taaacgtgca taatcaaaaa
       61 gacagattgt aggtggaata ttcgaacata acagttcaat tcatccttaa caatctgtct
      121 ttatattttt agtctctaat atgttgcact tgagctaaat attcaattgt ttgtttactt
      181 tcaatgcgac gtgcttttct tcgttcaaca cgttgtggtg ctgtatcata aaaccatttt
      241 tcaacatcat cttctggata tacaccaggt acatgtttag gtttgccttc atcatctaac
      301 gcaacaaatg ttaggtaact taatgcagct aaatcatgct tgttattaaa tacatcgtca
      361 attctaattt gaacgaccac ttccattgaa ctagtcccag cgtatgaaac catcgctacg
      421 tattgtaata tgtcccctgt tttaatcggc tttaagaaat ctactgagtc tgtagatgcg
      481 gtaactactt gcgcaccagc atgtttcata gctgtgattg ctgcaatttc atcaatatta
      541 gccatcaatg taccaccaaa cattgtatga tggtgattcg tatcttgagg gaaaacttgt
      601 ctatttttat aacattttga ttctgacata gatttcattg gtctgtcctg atttgtcatt
      661 attttatctc ccaatctata ctttaatcac gtatttgacc tttaaataca tgtttcattt
      721 cctactcagc acctcaaaat acatttatta aaatgttgat gcccaatttc cattttcaaa
      781 tactagttct tttgaaccat cttcgaatat gccataaata gtcaaatcac tactaccaat
      841 catgaaatcg acatgtacgt ttgaatcatt taatccgctt gcaatctttt cctcaacagt
      901 catttccgtt ccaccttgaa tattaaaggc gtaagcagat ccaatcgcta aatgacatgc
      961 tgcattttca tcaaataaag tattgtaaaa aatggtatta cgatttgata tcggtgaatc
     1021 atcaggtact aatgctactt cacctaatct tcttgaacct tcatcagtat tgattaaatc
     1081 tttcaatacc gcttcacctt tttcagctga aaaatcaata atctctccat ctttaaacat
     1141 caatttaaat tgatcgataa ttgtgccatt ataacttaaa ggcaatttat tagtaacata
     1201 tccatctact ctatttcgat ctggtgctgt aaatacttct tcagttggta tattagcaat
     1261 aaaagcttgt tccttgccgt taacataact tgtagcatct tcccagatat gatttttcgc
     1321 taaacctact gttaaatcag taccttcaga tacataatgc aacgcatggt aatttttttg
     1381 ttgtaacttt tgagcgtaaa cacttaaatt agcgatgtgt tgacgccaat tttcaactgg
     1441 atcgttacca tcaattcgca caatatcgaa tacctcgtca ataaacttaa tatatgcttc
     1501 ttccacagat agttcaggat acacactctt cgcccaagct ttagatggaa aagcagctac
     1561 gacccatgga aattgatttt tttgtacact ttccatatat cctttaaatg cgcgtgcgtt
     1621 ttgttgttga aatgcttgta atttttggct atcgatacca tccattaaat caggatcttc
     1681 actaataagt gctaaattag cagcgccacg tttaacgtaa tccattctcg cttcaacatc
     1741 atatgatttt atctcatgat tagcaaaatg ttccacagat tcattttcaa attttaagcg
     1801 ttttaaggtt ggatcgctat atacaacacg tacatctgaa gcgccacaat gatatgcttc
     1861 ttctacaatt aaatgcgtta attctaatgt ttctactgat gatctaataa atactggctg
     1921 ttttggttga acattcatac caaccttaac taatagttca gcgtattgtt gtaacttttc
     1981 tttgtaatta gtcaaaattc ccactcctag tcgtgtcgca attgtcctaa agcgtcagca
     2041 atagctgtca cttcacttgg cgacaatctt tctcttgatt gaacaaaatc atatatatct
     2101 gttaattctt cttcatttgc atttttatat ttttcaggat ctaataatcc ttgattaaca
     2161 atatttaact tctctctaat agctaatact ttctcttcat ttgtcatagc cacaatgttc
     2221 acctctaata atagttatgt tatcaaagta tcacaattta ataaacttta atagaatttt
     2281 atataatctg ctttcttatt gtttaaagtc gctataaaat ggggatataa ctacatagtt
     2341 tggaggaatc aaaatggtag atgtagcatt tgtctgtctt ggcaatatat gtcgttctcc
     2401 aatggcagaa gcaatcatgc gacaaagact taaagacaga aatattcatg atattaaagt
     2461 acattcaaga ggtactggta gctggaattt aggagagcca cctcatgaag gtacacaaaa
     2521 aattctcaac aaacacaata ttccatttga tggcatgatt agtgaattat tcgaagcgac
     2581 agatgatttt gattacattg tggctatgga tcaaagtaac gttgataata ttaaatctat
     2641 caatcctaat cttaagggac aattgttcaa actgttagaa tttagtaata tggaagagag
     2701 tgatgtacca gatccatact acacgaataa ttttgaaggt gtatacgaca tggtattatc
     2761 atcttgtgat aatttaatag actacatcgt aaaagatgca aatttgaaag aggggtagtt
     2821 ttttatggaa aataaatttg ttcctggtat tttaattggt gccgtaattg gtggtgcaat
     2881 tagtttagct gataaatcta cacgtcaagc tttagttcaa tcagttaaag atgcaaaaaa
     2941 tggtaaccgc actcgtaagc cttctaaagt cagcaagatt aaagacgaag ttttatactg
     3001 gaaagatgtt gttgaagaaa ttcgtcgtaa taatcctgaa ttagaacgtt cattaaaaga
     3061 tgcgaaagaa acatttgtta atagaaaaaa tcaacgctaa gatgattttg tttttataag
     3121 gcaatactta acaatttggt gtacgccttt catagttatc attattaaat gataatacaa
     3181 tgattggcca aactgcattg tttcagtatt gcctttgttt taattttaaa tcaaaatagc
     3241 ctatgaaaga tttaaatcaa ttaatttcta taatattatc atttttaaag catatcattg
     3301 tttagttttt ttataattgg ataaatacta atagttactt tataaaacat tacatagaga
     3361 aaggttaagg agtgcacatg tcgaaaaagg atcactcttc ttcaaaatat cttaattctg
     3421 ttaaggaagc acaagaggag tcaaaaaata aaagtaatcc caaaattgat gttgatcgta
     3481 catatattga acctcaacaa ttccaatcta agaaacctaa aaaagatgat caggttttct
     3541 tcttatcaag attaaataaa cctgcaaaat ataagaaaga ctctaatttc ttatcatatc
     3601 tcatctatcg cataggaaaa gatgatgcct caggactagc agcacaaatg acttaccatt
     3661 tcgtacttgc tatgttccct atgttgcttt tcctattaac attattacca tttttcaata
     3721 ttaagcagag tcaaattact aatatgttaa gcaatgcacc cgctgaaaca tctactctaa
     3781 ttaagagtgt aattggtgat ataactcaaa actccagtgg tggcttatta tctatcggtt
     3841 tgattttagc aatttggtca gcttcaaatg gaatgactgc aattatgaat tctttcaatg
     3901 ttgcttacga tgtagaagat agccgtaatg gaatcgtatt aaaactacta agtgttgtct
     3961 tcactgtagt tatgggcgtt gtgtttgtag ttgctctagc attaccaacg cttggttctg
     4021 taattagtca tttcctattc ggtccacttg gatttgacga acaagtgaaa tggattttta
     4081 accttattag aattgtgtta ccaatcatta ttatatttat cgtatttatc gtgttatatt
     4141 cggttgcacc taacgttaaa acgaagctta agtcagtatt accaggtgca gtatttactt
     4201 caattatttg gttagctggt tcatttggtt ttggttggta tatttcaaat tttggtaact
     4261 attctaaaac atatggcagt atcgcgggta tcatcatttt gttactatgg ttatatatca
     4321 caagttttat tataattgtc ggtgctgaaa tcaatgcaat cattcatcag cgtagtgtaa
     4381 ttaaaggtaa aacacctgaa gaagcagcat tagaacatga tgacaataat aaaaatcatt
     4441 ataatgagaa cactagatac gaatatgatg aagatagtaa tgcaacacat caacgtacgt
     4501 accatgttga tgaacatcct agtgatacct atccagaaga tgataaaaat ataaaagaca
     4561 aaatcgttga taagcttaaa aaagactaaa caccaacaaa acagaggttt tcgctaatga
     4621 ttgcgaagac ctctgttttt ggtgtctatt tgatgttata ttgtgattgg cgtaagtaac
     4681 ttttcttaat tcaatacgaa ctattgaatt aaattatgtt ggaatgcata gatgacagct
     4741 tgtgttctat cttgcacttc taacttactt aaaatgttac tcacatgtgt cttaaccgtt
     4801 ttaatagtaa tatgcgatgc actagcaatt tcttgatttg agtaaccttt cgcaatcaat
     4861 aataatattt ccatttctcg ttctgtaagc atttcatata actctgcgcg ctttttcata
     4921 cggttacgca ttttcactaa aacttccggt tcaaaaacag attctcctct agaagtttta
     4981 cgaactgcat cggcgatatc ttttgcactt gttgttttta aaatgtaact atcgacacct
     5041 gcatctaatg cacgatatac ctctttatct tcaataaaac tagttaacat taatacttta
     5101 atttgcggta aatctttttt aatctgagtc gtcgcttcta caccatccat gtcttccata
     5161 agtaaatcca ttaaaattaa atctggcttc aactcatggg ctttggcaat tgcttcctta
     5221 ccagaagcgc cttcaccaac tacttcaata tcactttgcg ttgatagata acttgaaatt
     5281 cctatacgta ccatttcatg atcatccaca aacaatactt taatcgtcat acgaatcctc
     5341 cttatttaaa ggtgctttca cctcgatacg tgtacctgaa tctggcaatg atacaatatg
     5401 gaacgttgca ccaatttcca aagctctttc acgcatattt ttaagtccat aactttgttc
     5461 taatttttca tcaacattaa aacctttacc attgtcttga attctcaaca ataaataatc
     5521 gtctttatta aacaattcta ctgtcacttt tgtaccgttt gaatgacgca atgtattcga
     5581 aattgcttcc tgtgtaattc tgaacaaatg atcttcaata cctttaggca ctttaaaatc
     5641 ttgtatttca tgcacaactt tcatcggcac ttttttttgt aaatcaataa ctaaatcttt
     5701 aataccctca cctaaagatt tgtcttttaa accaagcggt cttaaatgta acagcaaagc
     5761 acgcatttct aactgcgaat cttgaaccat tttctctaaa atcggaattt gttggtctaa
     5821 tggtggttct aacttcgttt ctttgatagc agaaagcatc atacttgccg caaaaagttg
     5881 ctgactaaca gaatcgtgaa gttctcgagc tagtctttga cgttcatctt caataatctt
     5941 tttaactttc acatcattaa tattataatt ttcattggtt aaattttgag ttttaagtcg
     6001 caacttatgc aattcttgat ttaaaggtac gagtgtatgg tataaatcta acgtttcact
     6061 atatatttct atattttgat cattaatgcc aactgtttcg ccttccattg aacgctcaat
     6121 ttgcgtctta atccaatcat tttgctgatt gattttgtat gcgagtaccg aaccaacaat
     6181 aatacacaat aatatgatga tgagatttaa aaataaaaag actggtattc cgaatatttg
     6241 tgtataaaac ataccttgaa aatagatgat atttacaaaa actttatcga tgaatagaaa
     6301 tgcagctagc atgctatata ctaagatgag cattgaacca attgttctaa tgtagtggtt
     6361 catcgataaa tcacctctac gtctccgata aacgttgata cgtagatatt aactgtatag
     6421 ttatccggtt tcatcatttc ttcaatatga atattgttat tttcaacttt atatgatttt
     6481 tcattcacgt aagtacttcc ataaaaagca gctacatgta aattaatatt gtaattaacc
     6541 ggcaatataa cctgcacttt acctaaaatg tgtctaacaa caatggtatt attttcctta
     6601 atatttgcag cttttgttaa gtcaatatgt aggtcgccaa ttccatgttg aatttggaca
     6661 tcttcccatt tatatacata aactggtgta cgttgctcac caaaccactt ttgtttaata
     6721 aaagatggtg aagtcataac ctcatccgtc gcaactactt tttttggttt aaatttgtgt
     6781 attaaataac gcacaataag tagtaataaa aagacgaaca agatgatgat tgtatactta
     6841 ttagacaata atgtgaatgc aattaatagc gcgcctatcc aaaacgccaa aaggccacgt
     6901 attttatgaa aataaagata tcctacataa actaatacac atcccaataa tagaacgagt
     6961 aaaaagccaa ttttttcaaa aaatatgtag taaaaattgg caataatcat taatgcagta
     7021 aaaatgatca acatttgcgt tgatatatat ttgtgtgtca tgtttcgccg cctttctatg
     7081 ccacagcgtt catataattt attagatacg atgttttttc tgttgctatt ttttcagttg
     7141 aagtttgttg aatttgagtt tcaatttgtg cataatatgc ttcaacttta ttcaactctg
     7201 atttaatatt ttcaatttct tgattattga tttctcttgc acattgaatt tgaaatgctt
     7261 cagcaatatc aatatattta ttttctaatg ttagcattag actgtcaatt aacttgctaa
     7321 gttgttcttt tttagtaatt aaataacgca tataatcatc taaatttttc attttttcat
     7381 ctagcattaa aaggtagtgc ttgatattat ttcttactgt tgctctcaaa aactgttcaa
     7441 tataacgttc aacatagttc ataactatca ccttttataa taagttttaa aataccaaat
     7501 gcgctatgta tcttcgttga tgtcgatgat atgtttgctt aaaaactact ttagtacaac
     7561 atatttttga aatgacgcat tgattgtgtt cttaattatt ataaaacagt tccgaaacca
     7621 atatgaatag gctccggaac taaatgatgt tcatacttta gtcttagtat atgttgaact
     7681 attcttcttc aatttttgtc gttaaaatcg gaccatcctt agtcacgata accgtatgct
     7741 caatttgagc aacaaaactt ttatcgctcg tttcaaaagc ccattcattt ttaccttctg
     7801 taacaaatga tgcatttgat gagataaacg gttcaatagc taataccata ccttcagtta
     7861 ataatgtttt gtcttttgga tcaaagtaat taagtacatg tgctggtgct tcatgtaatg
     7921 ataaaccaac accatgacct gttaagtttt taatgacttt caaatcattt tgtctagctg
     7981 tattatgcac cgctttacca atgttactta acttagtacc cggttttact tttgcaattg
     8041 cattctcaaa tgccatcgtt gctacgtcac atactttttg tttcattgga tcatctgatt
     8101 ctccaacgac aaatgaaatg cctgtatctg cataatagcc attcttcaaa gccgatacat
     8161 caatatttac taaatctcct tcacgaatga cacgcttact tggaatccca tgtgccacct
     8221 cttcattgac actaatacac gtttgaccag gaaaattttc atcatgaatt ggcgcagaaa
     8281 tagcaccgta ttcttcaaat aactctttcg caatattatc aagctctttc gtagtgatac
     8341 ctggtttggt tgcagcttgc attgtattgc gcactttagc gcatatgtat ccaatttctt
     8401 ttaacgcttg taattcttct tctgttttta caatcatttt atcccgttcc ttttttatat
     8461 aattataact tattatagca aaaattcttt gatatactaa agatgttact ttagtatttt
     8521 ggaaagtgag tgagacatag tgtgataaag aaaaaatatt tctttatcgt tgtaccccaa
     8581 cttgcattgc ctgtagaaat tgtagatcca atttctctat gttggggccc cgccaacctg
     8641 cacattatcg taagctgact tttcgtcagc ttctgtgttg gggcccacac cccaacttgc
     8701 attgcctgta gaatttcttt tttgaaattc tctatgttgg ggccccggac tataattgaa
     8761 aaatgcttgt tacaagtgca ttttatttca gtcaactact aacaatataa cattgtggag
     8821 cccagaactt tgattaatgt atatgaaaat caaagtaatg cttaagtatg attatttcaa
     8881 atattttaca tacatgaact tttccaatgt acgatactat tattataaag cgctcgctaa
     8941 gattttacga tgattagagg ttaaaaaatg aacgatcaat ggtataaaca tttaattggt
     9001 gccagaacaa ttaaaactgg tattgccatt tttttaacag ctgtcttttg tatggcacta
     9061 gatttaacac ccatctatgc cattttaaca gctgtagtca caattgaacc aactgccaag
     9121 gcatcactta ttaaaggtta tcgtagatta cctgctacgg taattggtgc aggctttgca
     9181 gtattattta catatttatt cggcgatcaa tcacctttta catatgcatt gagtgcaacg
     9241 ttcacgattt tattctgtac aaaactcaaa ttgcaagttg gtacgaatgt cgctgtacta
     9301 acatcattag ctatgattcc aggtattcat gatgcctaca tattcaactt tttgtctcga
     9361 acattgacag caattattgg actcgtgaca tcaggtttaa ttaacttcat ggtgtttcca
     9421 cctaagtatt atggtcaggt cgaagaaaaa ttaagtaaga cagatgcctt gatgtataaa
     9481 ttattttaca atcgctgcca agaacttatc ttatcaagac tgcaatctga taaaagcgaa
     9541 aaagcatata aaaatatttt caatttaaac aatcaagttg aaacgttaat aagttatcaa
     9601 cgtgatgaat taagctatca taagaaaaaa gaatgtgatt ggaaattact caatcaatta
     9661 actaaacgtg catatacgaa tcgtttgttt atcactcatt tatcaaatat catttactta
     9721 cctaaaaata ctcgtgttaa cttttcaggt gacgaaaaaa tggcattgct aaaaattagt
     9781 agtagcatca aagatatatt ttatgatggt tcttttaaga gagaagatga ttctgtagaa
     9841 actttacgtt cgacaataaa agcacttgaa attagtggtg aaaatcaaat taaaagtcat
     9901 atactatacg aagttctaat gatttatcga ctgctagaca gcagatatgc ttaatttaag
     9961 taccttatac taattggcac catgggagtg ggacagaaat gatattttcg taaaatttat
    10021 ttcgtcgtcc caccccaact cgcattgcct gtagaatttc ttttcgaaat tctctgtgtt
    10081 ggggcccaca ccccaacttg cattgtctgt agaaattggg aatccaattt ctctttgttg
    10141 gggcccctga ctagaattga aaaaagcttg ttacaagcgc attttcgttc agtcaactac
    10201 tgccaatata acttcgtaga gcatagaaca ttgatttatg tccccgcctc tttttatcgt
    10261 atatagcatg tcccttttta aaattacaag cataaatatt cagactcaaa tatagccaca
    10321 cccctaaact acggagggat gtggctgtct tttttgattg taattatgta tcagatttaa
    10381 atcattaatg taaagaggct aattcattaa taaatttagt aaaatctgat ttagcaacat
    10441 ttatccttaa tgaaatacca gattctgttg ccatgtttga atgccttaaa ccagaatcaa
    10501 aatcaatata aatacaaagc agttcttgat taaaatcaat aattgtaaaa tgtaaatcag
    10561 gttctataaa atatatttca gtaacaggat tgtcgtaatc catatgtttc aaattgtcca
    10621 tgtcactatc aaaaagtgca aattcaaatt taacttcttg atcttcgaaa gttgcactag
    10681 tgtcgaaata ataaatttta ttatcttctt cgataactac atcttttaaa ataaatgtta
    10741 tcgatgtttt gctatgtaaa taagatgcat cttttaatat caatttagta ggcttcatcg
    10801 tcaaattcct ttcatcattc attttttcat gttgcataat caccattact attgataatt
    10861 ctaggataga tactcattgt ataataatta ttcactagaa caaaaaaaac acacatcaat
    10921 ttggttgttt ccatccaaaa taatgtgtgt gtttatacat atatgattaa ttactaaact
    10981 tcatcgttta tcaatattaa atccattgat gcgatgttca gagttaacga gatttcttct
    11041 gtctatttgc tctgtctatt aatacttgtt tcgcttgtat ttccgcttca ttatctattt
    11101 ctttaggctc aaacggaata cccttacgtt cacaagcttt ttctaacaga taatcagtga
    11161 tttcgtaatt tttaggtaaa attggtccgt gtaaataagt acctaataaa tttttataat
    11221 gaatgccttc ttttttatct tcatcattat taccataacc aaaagtgaca tgaccaagtg
    11281 taccgaaatc atgatatgtt ctaccaccgt gattttcaaa acctacaata gttccaaaag
    11341 tatcactttc gataacaata tctcctgtta atcggtttgt ctttgattca gtataaaaat
    11401 ctaaaatacc taacccttct aattctgtac catcaggcgt gatatatttt tttcctaaaa
    11461 attgatagcc tccacaaatt gttaatcccg gcataccatc ttcaatcgct tctttaagtg
    11521 gtgtcttaat tttacttaat tcttttgttg ctaatgcttg ttctctatca cttccaccac
    11581 cgataaagaa aatatcacat tcatcaaagg taataccttc tgtttcattg atttctacga
    11641 cattaacttt aatatttcgt tttttagcac gttgtcttaa agcaataata tttcctatat
    11701 cactgtataa attcaattta tctgacataa aatgataaat agtcaattca tgcatattat
    11761 gattgacctc cttcaaacga acggtttaat tgttcaagca taggcgctaa tgatgtatag
    11821 tttggtattg caactgtgaa acctttataa tccatagtct ttgccgttgc tttataaata
    11881 tcacgctcaa ctataattgg tacttcaacc tctgctaact tcaatcgcaa ttgaagttct
    11941 tctgctcgtg tacctgtcac gatgatagct tcaatttgtt gcttagataa tttttcaaaa
    12001 tctgcatcat aaatccatga agtatctcga ccatctgcag cgttatcatt tagcgaaata
    12061 acatacactt tttcgccttc taattgttca ccaactgata aacttgcatt cattcctgca
    12121 ggatttttag ctaaattgat catcgcttct tttcgttctt ttttaaagta ctgcatacga
    12181 ccattgtctg atgtatacgt ttcaaagcca tttttaattg cttgttcatt taaccctagc
    12241 tctcttaaaa cagtatatgc tgctaacgcg ttataagcgt taaagtcacc tgcaattttc
    12301 atatcatatt tttcatcatt gatatttaaa tgtaaaaacg gtgccacatc aaaacttgat
    12361 atttcatatt ttgcttgctc tcgtttgaaa ccacactgac agtgataatg accaatttga
    12421 ttataatgaa tataatcgta ttgcaataag cgaccacagt ttggacaata tctactttca
    12481 ttcatcgtac tttgttcaaa ttcatgggca tgtgctttca taccatagta cacaatcgta
    12541 tcacttgcga ttttcaaacg actcacaaat ggatcatcag catttagcaa taatttgatg
    12601 cctttattac taattgtctc tgcaatgtta ttaaccataa tatcaatttc accgaagcga
    12661 tccatttgat ctctaaagaa attagtaaat accatcattg aaggtgtaac ttcttttaac
    12721 acacgtggaa tcgaaccttc atcaatttcg attaccgcaa ttttagtctt aggtgttgat
    12781 tgcatgatga atgcagaagt tatacctgca gccatattag caccttcatt attgtgtata
    12841 atttgaatat tatttgcttt taaagtatgt ccaattaagt ttgaagtcgt tgttttacca
    12901 tttgttccac tgataaatac aatatcatca acttgctctg ctaattttct taatatatct
    12961 gtatccactt ttctagcgat ttgtccaggt aaatctgttc ctcttttacc tactgctcta
    13021 cttgctttac gcgccaattt cgctagatgg attgccgtcc actgtctcat gtgtttcctc
    13081 ctcaaatttc cactcgcatc attataacat gacaaggcaa cttcaaaaaa gtttctcaat
    13141 cacaaattga tacagtgttt ctaaattata atcattctca tttagataat taaaaatgat
    13201 atttattctc aattaaatat cgctaa
//
LOCUS       DAMZLE010000065        13185 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMZLE010000065 DAMZLE010000000
VERSION     DAMZLE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.065"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            61..825
                     /locus_tag="POB92_001626"
     CDS             61..825
                     /locus_tag="POB92_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6579127.1"
                     /translation="MKRLKKIVLCISFLFLTIFIGGCGMGKEAEIKKSFEKTLSMYPI
                     KNLEDLYDKEGYRDDQFDKKDKGTWIINSEMATQNKGEALKIKGMVLYMNRNTKTTKG
                     YYYVNAIKNDKDGRPQENEKRYPVKMIDNKVIPTKEIKDENIKTEIKNFKFFVQYGNF
                     KDLKNYKDGDISYNPEAPIYSAKYQLTNDDYNVKQLRERYDIPTNKAPKLLLKGSGNL
                     KGSSVGYKNIEFTFVEEKGKNIYFSDSLDYKKSGEV"
     gene            828..2204
                     /locus_tag="POB92_001627"
     CDS             828..2204
                     /locus_tag="POB92_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579128.1"
                     /translation="MAKAEYEIDPGKITSNSEETSAISNISYEIENANDNNLDNEKIR
                     GQITKLKKGGNFPKNLDYIDSYTDPSTGTTATAFLNKDTGKVTVGMAGTNFHGDQLKR
                     VALSSMSPLLFPPSKQDMKDVRGTMKDGVADLAIGVGMVNYKGKHFANTQQFIENLQK
                     KYEIDTVTGHSLGGRDAIFLGLRYNIKNVVAYNPAPLEVKSIRNKFGGQLFRNTTFPD
                     EKYLKELMDNYDGDITKVITQKDGLDYLVKRTDHLTCGDVLRINNGQGHAMENFLGEK
                     EQREIIEELMLVKGYRDANDKAFKALKKNTEKKLGKIDEIKSKLLQTNGGALSSSQQK
                     LLETLVAFSVAEGLSKMVDQELQQLKNMFNLMDEKFEANWKDAQEASDIVGKHLSYPE
                     KVSALDNGGVNESKLATEPHNEIKDKLNKITDLSKKYNSYLQQIEKSINEIVAKDQQL
                     AGQIGDLI"
     gene            2204..2530
                     /locus_tag="POB92_001628"
     CDS             2204..2530
                     /locus_tag="POB92_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579129.1"
                     /translation="MFEEYKKIDDLENAYEIELKRIEREIQNLSDLKYHLRRENEQSY
                     DAFLYLKNKMNYSEESNAKVRRLVEEFDYEADTYIRQKELKLEDYKEEIRREYIQQSE
                     KIMEAK"
     gene            2534..2845
                     /locus_tag="POB92_001629"
     CDS             2534..2845
                     /locus_tag="POB92_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579130.1"
                     /translation="MGIPDPLSNPMSDPLLGDSWMVGDLKKRQEKKLITTVIAPEHDE
                     KHRENKSKMKKSEKQLINQLINHGHTFKSDFKNVAKGDWVNKAMQDLDKLGNELKKIM
                     N"
     gene            3097..5334
                     /locus_tag="POB92_001630"
     CDS             3097..5334
                     /locus_tag="POB92_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6579131.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHNALIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYFIDLIHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            5485..6663
                     /locus_tag="POB92_001631"
     CDS             5485..6663
                     /locus_tag="POB92_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6579132.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNIGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIETEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6665..8053
                     /locus_tag="POB92_001632"
     CDS             6665..8053
                     /locus_tag="POB92_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579133.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(8540..10201)
                     /locus_tag="POB92_001633"
     CDS             complement(8540..10201)
                     /locus_tag="POB92_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI6579134.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(10525..12300)
                     /locus_tag="POB92_001634"
     CDS             complement(10525..12300)
                     /locus_tag="POB92_001634"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDI6579135.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKKAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYGVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPQKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            12651..13124
                     /locus_tag="POB92_001635"
     CDS             12651..13124
                     /locus_tag="POB92_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDI6579136.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT         4586 a         2035 c         2394 g         4170 t
ORIGIN      
        1 acgttgaaaa tatgtaattc acaaaagcaa atcatataaa aagaagagag ttgtgatacg
       61 gtgaagcgtt taaaaaaaat agttttatgt attagttttc tatttttaac aatttttatt
      121 gggggttgtg gcatgggtaa agaagcggaa attaaaaaaa gttttgaaaa aacgttgagt
      181 atgtatccga ttaaaaatct agaagattta tacgataaag aaggatatcg tgatgatcaa
      241 tttgataaaa aagataaagg cacatggatt attaattctg aaatggcgac tcaaaataag
      301 ggagaagctt tgaaaattaa aggtatggtc ttatacatga atagaaatac aaaaacaaca
      361 aaaggatatt attatgtaaa tgcaataaag aatgataaag acggaagacc ccaagagaat
      421 gaaaaaaggt acccagttaa aatgattgat aataaagtta ttccaacaaa agaaattaaa
      481 gatgaaaaca taaaaacaga aatcaaaaac tttaagttct ttgttcaata tggcaacttt
      541 aaagatttga aaaattataa agacggagat atttcatata atccagaagc cccgatttat
      601 tcagcgaaat atcaactaac taatgatgat tataatgtaa aacaattacg tgaaagatat
      661 gatataccga cgaataaagc gccaaagtta ttgttaaaag ggtcagggaa cttaaaaggt
      721 tcatcagttg gatataaaaa tatagaattt acttttgtag aggaaaaagg taaaaatata
      781 tactttagtg atagcttaga ttataaaaaa agtggagaag tataattatg gctaaagcag
      841 aatatgaaat tgatcctgga aaaataacaa gtaattcgga agaaactagc gcaatatcta
      901 atattagtta tgaaatagaa aatgcaaatg ataataattt agataatgaa aaaattagag
      961 ggcaaattac taaattaaaa aagggtggga attttcctaa aaacttagac tacatagata
     1021 gttatacaga ccccagtacg gggaccacgg caacggcatt tttaaataaa gatacaggta
     1081 aagttactgt tggaatggct ggtacaaact ttcacggcga tcaacttaaa agagtagcac
     1141 ttagttcgat gtctccgtta ttattcccac cctctaagca agatatgaaa gatgtacgag
     1201 gcactatgaa ggatggtgta gcggatttag ccattggagt aggaatggtc aattataaag
     1261 gaaaacattt tgcaaataca cagcagttta ttgagaattt acaaaaaaag tacgaaattg
     1321 ataccgttac tggtcattct ttaggaggga gagatgctat ctttcttggg cttcgttata
     1381 acatcaaaaa tgttgtggca tacaatcctg ctccgttaga agtaaaaagc attcgtaata
     1441 aatttggtgg tcaattgttt agaaatacga catttccaga tgaaaaatat ttaaaagagt
     1501 taatggataa ttatgatgga gatattacta aggttataac tcaaaaggat ggattagact
     1561 atttagtgaa gcgtacagat catttaactt gtggtgatgt gttacgtata aacaatggtc
     1621 agggccatgc gatggagaat tttttaggag aaaaagaaca acgtgaaata atcgaagaac
     1681 taatgttagt aaaaggatat cgcgatgcaa atgacaaagc atttaaggct ttgaaaaaaa
     1741 atactgagaa aaaactcggt aaaatagatg aaatcaaatc gaagttactg caaacgaacg
     1801 gtggcgccct ctcgtcttct caacaaaaat tgcttgaaac tttagtggca ttttcggtag
     1861 ctgaaggact aagtaaaatg gttgaccaag aacttcagca gttgaaaaat atgttcaatc
     1921 ttatggatga gaaatttgaa gcaaattgga aagacgcaca agaagctagt gatattgtag
     1981 gtaaacatct ttcataccca gaaaaggtaa gtgcattaga taacggtggt gtcaatgaaa
     2041 gtaaacttgc taccgaacca cataatgaaa taaaagacaa actaaataaa attacggact
     2101 tatctaagaa atataattcg tatttacagc aaatcgaaaa aagtattaat gaaatagttg
     2161 ctaaggatca acaattggca gggcaaatag gtgatttaat atgatgtttg aagaatacaa
     2221 gaaaatagat gatcttgaaa atgcatatga aatagagttg aaacgtattg aaagggagat
     2281 acaaaatctt agtgatttaa aatatcacct tagaagagaa aatgaacaaa gttatgatgc
     2341 atttttgtat ttaaagaata aaatgaacta tagtgaagag tcaaatgcta aggtgagacg
     2401 actagttgaa gaatttgatt atgaagcaga tacttatatt agacaaaaag agttaaagtt
     2461 agaagattat aaagaagaaa taagaagaga atatatacag caatctgaaa aaataatgga
     2521 ggcgaagtag cttatgggaa tccctgatcc gttatctaat cctatgtctg accctttact
     2581 aggagatagt tggatggtag gtgatttgaa aaaaagacag gaaaaaaaac taatcacaac
     2641 agtcatcgca cctgaacatg atgaaaagca tcgtgaaaat aaaagtaaaa tgaaaaaatc
     2701 tgaaaagcaa ttgatcaatc agttaattaa tcatggccat acatttaaga gtgattttaa
     2761 aaatgtagct aaaggtgatt gggttaataa agccatgcaa gacttagata aattaggcaa
     2821 tgagcttaaa aaaataatga attagttgaa ttgtcacata ctttttcaaa ccaacaacga
     2881 actaaaaaat ccatataaat atgattaaaa gaagtcataa ccattcgtaa aggatggtta
     2941 tttttaatag taaacaaaat tgctgctaac ctacatcttg aaccaagtga ggataaataa
     3001 cctcctgcct cccacacgat tcaacacgaa tttcaaatat attgctgtaa aataatgtaa
     3061 aataaacgta ttcatattac tgaattgagg gatagtatgc aacgtgatta tttaattcga
     3121 gtagaaactg aaagcatgcc agatttcaaa aggctcaatg gtttaatgat tggttttgtt
     3181 attaaaggtg aggcacatat ttatgatgaa aataacatga cgcaatgcaa cagtggcgac
     3241 attttcatca ttaaccaccg tgacttgtat cgatttcaac ttcaacaaga tggcatcata
     3301 tgttatatcc aattccaaat gaaatatttg gcagacaagt ttgatgatgc gcattgtcta
     3361 tattttcact taacagatgc gaccacaacc aagaatatac atcaactgag aaatataatg
     3421 gcaagactgg tttcaacaca cattcgacat aatgagttgt ctaaattgac tgagcaacaa
     3481 cttgtgattc agttgcttat gcatatgatt cattatgtcc cgcgtacata tcattcgaac
     3541 caaagtatct taaatgatga taaagtgaat caagtatgcg actatatcga gttacatttt
     3601 catgaagatt taagcctttc agaattaagc gaatacgttg ggtggtcaga gagccatctg
     3661 tctaaaaagt ttacagaatc gctaggtgta ggattccaac atttcttaaa tacgacgcga
     3721 attgagcatg cgaaactcga tttaacatac acagatgaaa cgattactga tattgcattg
     3781 caaaatggct tttcaagtgc agcgagcttt gcgagaacat ttaaacactt tacgcatcaa
     3841 acgcctaaac aatatcgagg tgatcgtcca gcaatcactg aaaatcaaca atcggcacaa
     3901 cataattatc acgaccgtga attgatatta cttttaaatg actacattga agaaatgaat
     3961 catttcattg aagatattga aaagatgaac tataaagaga ttgcctttaa accaactaat
     4021 caacaactaa atcaatttaa tcatattatt caagtgggct atttgaggaa tttgctcaat
     4081 acacagtatc aatcacagtt gcttacatgt catcatgatt ttcaagtcaa tgaagtatta
     4141 gcatatgatg tgatgccata tattatgaaa aagctcaatg cgccattcac gtatgatgca
     4201 gagatttcga atatatttta tgatatcgat ttgtgtttag actttttatt agatcataat
     4261 tttagtctaa ccatgcattt gaatcagtat gactcacgag attatatcga tgcattcaaa
     4321 gtatttatcc atcacgttgc cctgcatgtc agtcatagaa aagatttgaa gttcaacttg
     4381 tatgtgacga cattgcacaa tgctttgatt gaaatgattg attattttaa agcgttattc
     4441 cctaatggtg gcttgtacat tcacttagat caagctacgg aaagacatct accattgttg
     4501 aaacgacttg agccacacat cgaccatttt gtatttgatg ccaattcaaa tgatgctgtt
     4561 gattttaata aaatgaatga tgatgaattt aaaaccgcga gtcaaatgat tattaataaa
     4621 acgaattact ttatcgactt aatacatcgt cataacctaa agcgtccact cattttactc
     4681 aattggaata cattgacggg tgatacattt ataaccaacg gcgaatattt tagaggtggt
     4741 atcatcattg agcagttatt aaagttaagc tctaaagtag agggtatcgg gtattggttg
     4801 aattatgatt tgcacgttag tcattgtaaa aatgaacggg attatatgaa ttctattgaa
     4861 ctgtttcatc aatataatgg aaaacgtccg gtctatttca cggcattgct atttaataaa
     4921 ttaacaagca atattttgta ttctgatgat acatgtattg tcacgggaac tgattcaaat
     4981 tttcaaatat tgttatatga tgcaaagcat tttaatccgt acttagcgtt ggacaatcaa
     5041 atgaatatgc gtgcaacgga aatgatccat ttgaacatta atgccttgga agaaggtatg
     5101 tataagatta aacattttac cttagataaa gaaaatggtg cgttatttaa tctttggcgc
     5161 aaacatcata cgatacatgg catggacaag gactctatag attacgttaa tcgaatgagt
     5221 tttccgaaat tagaagtata tgatatagat atcacggaca cactggcatt aaacattaaa
     5281 atgattacga atgggattca cttaattgaa gtaaaacgtt acccaagttc ataaaatgat
     5341 cacaaatcac aaattttgat atacataatt tgtgattttt tatattcaaa gctaaaattg
     5401 caaaaaatta atggttaaca tctctgttgt tggcaatata aataatgatt aatcatttat
     5461 gatgtaacta aggagatgaa ggatatgaat caacaattaa ttgaaacttt aaaatctaaa
     5521 gaaggcaaaa tgattgagat cagacgttat ttacatcagc atccagaatt atcttttcat
     5581 gaagatgaaa cggcgaaata catcgctgaa ttttacaaag gtaaagatgt ggaagtagaa
     5641 acgaatatcg gaccacgtgg aattaaagta acgattgatt cagggaaacc tggtaaaaca
     5701 ttagcaatcc gtgcagactt tgatgcatta cctattactg aagatacagg attatctttt
     5761 gcatcacaaa ataaaggtgt tatgcacgca tgtggtcacg atgcacatac agcatacatg
     5821 cttgtattag cagagacgct tgctgaaatg aaagatagtt ttacaggaaa agtcgttgtg
     5881 atacatcaac cagctgaaga agtaccacca ggtggtgcta aagcaatgat tgaaaatggt
     5941 gtattagacg gtgttgatca tgtattaggt gtacacgtca tgagcacaat gaaaacaggt
     6001 aatgtgtatt acagacctgg ttatgttcaa acaggacgcg catttttcaa attgaaagtt
     6061 caaggtaaag gtggtcatgg ttcatcaccc catatggcca atgatgccat tgttgcaggt
     6121 agctacttcg tcacagcgtt acaaacagtt gtatctagac gactaagtcc atttgaaacc
     6181 ggtgttgtca caatcggttc atttgacggt aaaggtcaat tcaatgtcat taaagatgtt
     6241 gttgaaattg aaggtgatgt acgtggatta acagatgcta caaaagcaac aattgaaaca
     6301 gaaattaaac gtttatcaaa aggattagaa gcattgtatg gtgtaacttg cacattagaa
     6361 tataacgatg attatcctgc attatataat gatccagagt ttactgagta cgtggctaag
     6421 acgttaaaag aagcaaacct tgattttggt gtcgaaatat gtgaaccaca accaccttca
     6481 gaagactttg catactatgc taaagaacgt ccaagtgcct ttatttatac aggtgcagct
     6541 gtggaagatg gtgaaattta cccacatcat catcctaaat ttaacatttc agaaaaatca
     6601 ttacttattt cggcagaagc tgtagggaca gttgttttag attaccttaa aggagataac
     6661 taacatgaat gaaacgtatc gcgggggcaa caagttaatc ttaggtattg tattaggtgt
     6721 tattacattt tggttgtttg cacaatcact tgtaaatgtt gtaccaaatt tacaacaaag
     6781 ttttggtgca gacatgggaa caattagtat tgcggtaagt ctaaccgcac tattttcagg
     6841 catgtttgtt gttggagcag gcggcctagc agataaaatt gggcgcgtga aaatgacgaa
     6901 tatcggttta ttgttaagta ttattggttc agcattaatt attattacga atttaccggc
     6961 attattaatt ttaggtcgta ttatacaagg cgtatcagca gcgtgtatta tgccttccac
     7021 attggccatt atgaaaactt attatgaggg tgctgaacgt cagcgcgcct taagctactg
     7081 gtctatcggt tcttggggtg gaagtggtat ctgttcactc ttcggtgggg cagttgcgac
     7141 aactatgggt tggagatgga ttttcatctt ctcaattatc gttgccgtac tttcaatgtt
     7201 actcatcaaa gggacgcctg aaacgaaatc agaagttacc aatacacata aatttgacgt
     7261 tgcagggcta attgttctag tagttatgtt gctaagttta aacgttgtca ttactaaagg
     7321 tgcagcactt ggttacacat cattatggtt ctttggttta attgcaatcg taattgtagc
     7381 attctttatt ttcttaaaag ttgagaaaaa agtagataat ccgcttattg attttaaatt
     7441 atttgaaaat aaaccatata caggtgcaac gatttcgaac ttcttattaa acggtgttgc
     7501 aggtacatta attgtagcga atacattcgt gcaacaaggt ttaggttata cagcattgca
     7561 ggcaggatac ttatcaatta cttatttaat catggtgtta ttgatgattc gagttggtga
     7621 aaaattatta caaaaaatgg gttctaagcg accaatgtta ttaggtacat tcattgtggt
     7681 cattggtatt gcacttattt cattagtatt cttaccaggc atattttatg ttattagttg
     7741 tgtcgtagga tatttatgtt tcggactagg cttaggtatt tatgcaacac cttctacaga
     7801 tacagctatt tcgaatgcac cgttagataa agttggcgtt gcttcaggta tttataaaat
     7861 ggcttcatca cttggtggtg cattcggtgt cgcaattagt ggtgcagtat atgccggtgc
     7921 agttgctgca actagcattc atacaggtgc gatgattgca ctttgggtta acgtattaat
     7981 gggaatcatg gcatttatcg caattttatt cgcgattcct aatgatgata aacgtgtcaa
     8041 agatgcgaaa taataacgat gacacgccaa gtgttttgat aatgatataa atgaaagtaa
     8101 agcaaattaa atgaggaaat gtatagagca gtgcatatag ataaatctat gattggatta
     8161 acactaatat aaatataaat gaaaaaccat ccacttaaac ttttgataag ttaggtggat
     8221 ggttatttat tgtgttgttc taaaaatttg gacatttata tagaaggaac tatatctaat
     8281 gtgaagataa tggctattcg cttcaatgaa ggcgacgtgt aaaagtgtat attaaacgtt
     8341 tcggggtcag actccgagaa cagtgtccgt catgcttacc gcatactaat gaagcattat
     8401 tatatcagtg tttatagccg atttaagata ataaattttg ctaaacaaaa aagccaaccc
     8461 atgaatgttg gattggcttt ttacatgcat ctgaatctct aattttaaaa aaatatgaat
     8521 ataaataaga cagtaaaaat taaatttcag ttgttgcaat ttcttcatct gtaggtacat
     8581 catcgttaag gccaacaagt gcttcagaaa catttcgtga atgataaccg atacgttcaa
     8641 gaacaccaat catatcgata tatagtaatc cgccttttgt tgtacattca ccacgattaa
     8701 ggcgtttaat atgacctttg cgtagtttat gttcaatatt aaatgattct ctactacgtt
     8761 ctacaatttc atcttttttc gttttgtcat aaacatctaa catgtcgatg gctttatcaa
     8821 atgactcagc aacatggttg aataatttat ccataccgcg ttgtgcatct tctgtaatgc
     8881 gaatatcttc atcatgttgg cgttttaatt gagcgacata ctcttctgtt agctctgcta
     8941 cttttaaaat agagcgattg acatcaaaca taactgctaa acgctcaacg tcagccttcg
     9001 ttatggcttt tgtagaaatt ctaactaaat aatttcgaat gctatcattg attgtttcaa
     9061 cagcttggtg cttttgttca agctttttga tcaatttttt atcgtctttt gtaatttcgc
     9121 gaatgtcttc aaacattgat aagacaatct gaccaacatt ttgtaattct ttttgagttt
     9181 cttgtaatgc aacaccaggt gcgtgataaa caagatcttt gtttaagtgc tgaggtttat
     9241 agtcatcagc aatatcttta cctgggacaa gctttgtaac aatccatgct aaacctgcta
     9301 caaatggtaa ttgaatcaaa gtatttgtta tgttgaagat accatgtgat actgcaatcg
     9361 tcatcgctgg ttttaagtgc catacatctt gtaacaaact aatcaaatga atcacaactg
     9421 gcaagaaaat tgtgaagata attaccccga ttaagttaaa gatgacgtgt acaagcgccg
     9481 cacgttttgc agcgattgag ccggctaaac tagctaagat agctgtaatc gttgtaccaa
     9541 tattatcgcc tagtaacaca gggattgctg cgtttaagct aattaaatct tgttgataaa
     9601 attcttgtaa aataccaatc gtcgcacttg aactttgaac tagtgctgtt aaccctgcgc
     9661 cgacaataac agcaagtatt ggatttgatg acatatcaag cattaattgc ttaaatccat
     9721 ctaatgatgc taaaggttta acggcatcac ccataaattc tagaccgaag aatagagaac
     9781 cgaaaccgaa tagtatgcgg ccaatgttat taattttaga gcgtttaaag aaaaagatta
     9841 aaaatgcacc taatgctaaa attggcattg catattcgcc taaatctata ccgataataa
     9901 atgcagttac cgttgttccg atattagcac ccattatcac tccaatggct tgtttcaatg
     9961 tcataaatcc agctgttacc agtccgattg tgataactgt cgtacctgaa ctactttgta
    10021 ttaaaatagt tacaacgata cctgcaataa cacctaatac tggatttgat gtaaatttgt
    10081 ttaaaatatc tcgtagcctg tctcctgctg atgcttgaag cccgtctccc atgattttta
    10141 agccgtaaag gaaaatacct aaaccaccta aaaaggagaa aatgacttct gtaaccgaca
    10201 tttccattat ttcacctcaa ataagcttta tatttagatt atcgcttata attgtaaatt
    10261 taatgttaag attaggtaaa attatttaac aatatatgtt atttgtatat gacttgtaaa
    10321 atatcgtcac ttattatgta aattttcagt gtgaaatggc aggtttgcaa tcacttgttt
    10381 aacaaaatga tgcaatcaat catgtaatta tgtttcatca aaaaaatcat gtgagtggga
    10441 taacgaaata aagtttgtga tcatatcact tctatcccac tccatgattt gaaatcacca
    10501 tatagacaga tttaatgtgc ctcgttataa caatttgtgt tcttttaata atgactcaat
    10561 gtacgtacct tttatctttt taaggaatcc tgctaatgcg agtttttgca ttttcgaatc
    10621 tttagtaatc tcgcgcaaat cttgatggtc attcagttcg tatatggcat ccattaagac
    10681 gcgaagatca aatgtactat tgatgacctc tggaatacca cgatctatat ttagtaattg
    10741 ataaacagct tccatggcag tacgaaccga atattctgtt gtaaatacag tatctcgctc
    10801 tgtttctgca aagttaccaa taaatgctaa attttgtgat tgatgcggga cgactaaagg
    10861 tctgtcgccg atagcacgcg tcatgaaata agaggtaata tatggcatat aaacaggaat
    10921 cgtattagat gcatgttttg ctaagtcttc aattttgtca gttggtacac ctaagtgata
    10981 cagccattct tgacatattt cattaccact acattctgtg attggctttt taatataatc
    11041 gccgtttaca tctgaatata aggcataaat ccatgtagat atttcatttt taggttggtc
    11101 tttaaactgt tgctgacgat tgattgtaaa actgatttgc catgcagaat cattgattgt
    11161 aataattccg ccagtaaccg ttttgcctgc aagagggtca cgtttacaaa tgctttctat
    11221 tgtatcgata atatctttat tgtttgttgt cgatgttgca gaaacaaacc aactcttttg
    11281 aggaatattt tggcaaaact tatcaggatt accaaattca ggactttgtc gcgctaaatt
    11341 tttccatagt gtccaactac cacctaattc gtcagttggt ggtgctggtg tatcattatc
    11401 accatacgta gagctttccg taatactacc gtttgtcaca aagacaagat catctacagt
    11461 cagtttaatt gactctgctt tgccatgtcg gtgaattaat atttctcggg caattttttg
    11521 acttgtcgta acatctactt taatatcttc tacttttaca ccgtattcaa attgaacccc
    11581 atgcgatttt aaatattcaa ccataggtaa tactaaagat tcatattgat tatatttagt
    11641 gaattttaaa gctgaaaagt ctgcgagacc accaatatga tgaacgaatc gcattagata
    11701 gcgacgcatt tccatagcag aatgccacgg ttcaaatgca aacatcgttt tccagtaaat
    11761 ccaaaagttt gaattaaaga agtcatcgga aaatacatct gttattttga catcatctaa
    11821 atcttcttca ttcgttaagc ataaatctaa aatttcttta atcgcttttt tggtcaaagt
    11881 gaagtctcca tcggtaacta aacgttgacc ctgtttttca ataactcgac agcgagaata
    11941 gttagggtct tctttgttta gccaatagaa ctcatctaat acagacgcgt tatcgatttc
    12001 taatgaaggg atagatctga ataagtccca caaacattca aagtggttct ccatttcacg
    12061 accacctcgg acaacatagc cttttaaagg catattttca ccatcaagac taccacctgc
    12121 tttaggtaac tcttctaaaa tatgaatctt cgaaccttcc atttgaccat cccttattaa
    12181 aaaacaagct gcagcaagtg aagctagacc agatccgatt aagtaagcgg atttgttttc
    12241 tacattttca ggttttttag ggcgcgcaaa tgcttcataa tttccataac tgtaatacat
    12301 atccaccaag tcctttcgta ttagaataca ctcagactat acccctttgt gaattgcaaa
    12361 tattagtatt tatattggta aatttgacga aattagatca tttttatatt tttgattttt
    12421 aaaattttat cactcgaatg tttttggatt ctgaggtgcg ggacttggaa taattcgaat
    12481 cattatgtgg catgttaaac aggtaaatat aagctatgca catttaacaa gcggatactc
    12541 ctgacgcaat tatagtatca ttacatattg tttaatcgtt tggggtaatg taattaaaag
    12601 gtaaaatagg cagagtgtta tacataaaat gtaaagaaag aggcgataga atgtctaaaa
    12661 tcaggtcttt tacaatatta agtctactta tttacttagc tatgatgtgc tatacagtag
    12721 tgacctattc aaaattacca accaaagtac ctattcatta taatttagca ggggatgctg
    12781 ataactttgc tgataaatgg gtgctgcttt tgattaatag cgcatttata gtgatttggc
    12841 ttatattttt cattgcaggt agatactatg aacgatttgc caaatggtca cattataatc
    12901 atacaccacg tgaaattcga gcgattaaat tatttttaag tacgttaaat ttagagatta
    12961 tgagctatat gtctatcttc acagtattag aaatttggca aatacaacac catcatcaat
    13021 tcaatttact atggtttaat atgatattta tcattatcat tggtttgacg cttgtcatat
    13081 tttgtttact tcctacaatt cataaaatga gagattctca ataaaaatat aaactgtcat
    13141 gatgcagttt tgaagcggat tcctgagatt attataaata atatc
//
LOCUS       DAMZLE010000066        12943 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMZLE010000066 DAMZLE010000000
VERSION     DAMZLE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.066"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(120..317)
                     /locus_tag="POB92_001636"
     CDS             complement(120..317)
                     /locus_tag="POB92_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579137.1"
                     /translation="MNCYDEIFNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(820..2160)
                     /gene="glnA"
                     /locus_tag="POB92_001637"
     CDS             complement(820..2160)
                     /gene="glnA"
                     /locus_tag="POB92_001637"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDI6579138.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2179..2547)
                     /locus_tag="POB92_001638"
     CDS             complement(2179..2547)
                     /locus_tag="POB92_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6579139.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2791..4029)
                     /locus_tag="POB92_001639"
     CDS             complement(2791..4029)
                     /locus_tag="POB92_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6579140.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEQVITRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4048..5286)
                     /gene="hflX"
                     /locus_tag="POB92_001640"
     CDS             complement(4048..5286)
                     /gene="hflX"
                     /locus_tag="POB92_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI6579141.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKMEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NCPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5398..5874
                     /locus_tag="POB92_001641"
     CDS             5398..5874
                     /locus_tag="POB92_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6579142.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6096..6328)
                     /gene="hfq"
                     /locus_tag="POB92_001642"
                     /pseudo
     CDS             complement(6096..6328)
                     /gene="hfq"
                     /locus_tag="POB92_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
     gene            complement(6343..7278)
                     /gene="miaA"
                     /locus_tag="POB92_001643"
     CDS             complement(6343..7278)
                     /gene="miaA"
                     /locus_tag="POB92_001643"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI6579143.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRGLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7296..8210)
                     /locus_tag="POB92_001644"
     CDS             complement(7296..8210)
                     /locus_tag="POB92_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6579144.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8360..10033)
                     /locus_tag="POB92_001645"
     CDS             complement(8360..10033)
                     /locus_tag="POB92_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDI6579145.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10191..11687)
                     /gene="glpK"
                     /locus_tag="POB92_001646"
     CDS             complement(10191..11687)
                     /gene="glpK"
                     /locus_tag="POB92_001646"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI6579146.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLSRGTEKEHFIRATIESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11816..12634)
                     /locus_tag="POB92_001647"
     CDS             complement(11816..12634)
                     /locus_tag="POB92_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDI6579147.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         3996 a         2508 c         1788 g         4651 t
ORIGIN      
        1 ataagtaaat cactaaacat gatttctttc acttttagaa aacttttaat actataaaaa
       61 cacccactca gtcactagtt tgggcagtta ttgtatgcct attgaactca atgcttatat
      121 tacaatacct ttttcgcata ttcatataag atctttgcat tctttaagct taaattttct
      181 attcttcttt ccccacggcg catagcatta atattaccgt aacttatccc agtatcttta
      241 ttaatttgat aacttgatat ctctttgttt tctatcaatt ttttgattgt attgaatatt
      301 tcatcatagc aattcataaa ttagatgagg cgaaattttt aattttttag aatatcaata
      361 gtagtataac taaaatgaaa ataccgatcg ataaacaaaa agatattttt tgttttgttt
      421 ctcttttcat atagtattac ccccttaata atgagtagta aggtccctct tttcggcgtc
      481 ttaccttaga acgttctgca aatgaattcg atgagaagta atatgatatg gctattttca
      541 agtaatactc aacgttttcg cgacgttctt ttatcgcctc atctcatcac ctccaaatat
      601 attaaaattc atgtgaacta aaatataaaa tggtcttccc cagctttaaa aaaataaata
      661 cacaaaacat ttacttgacc aaaacttgac caaaagttgc ccaatcgtaa caaaaggcac
      721 attttacacc caaaaaagtt gcccagttaa atcaaaaaag agctaaagca aaacgctcta
      781 gcccttgata ttactgattt cccatcattg tttagcgtat taatattgct tcatgtactg
      841 atctctttcc cattcagata cttgagttct gtagtaatcc cattcaattg attttgaatt
      901 aataaattga ttatagatat gatttcctaa agcttttttt ataacttcat tttcacgcat
      961 tgcttttaat gctgtgtaaa gtgttgaagg taagtcttga atgcctactg cttcacgttc
     1021 ttcacgattc atttcgtaaa tattttggtt aacaggttct ggaactttta atttattttt
     1081 aataccgtct aaaccagctt ctaaaattgc agctaacgcc atatatgggt tcgcagctgg
     1141 gtcaactgaa cgtacttcaa tacgagttga taatcctctt gaagatggta cacggattaa
     1201 cggtgaacgg tttttaccgc tccatgcaat ataacatggt gcttcataac caggaactaa
     1261 acgtttgtat gagtttacta atgggttaca taccgctgta aatccacgtg cgtttttaag
     1321 tacaccagct gtgaattggt aagctgtttc tgttaagccc atttcagtat tcggatcaaa
     1381 gaatgcattt tctttacctt tgaataatga aacgttaaag tgcataccgc taccattcac
     1441 accaaataat ggtttaggca taaatgttgc gtgtaaatta tgtttacgtg cgattgtttt
     1501 aacaaccaat ttaaatgttt ggatattatc acatgctgta acagcatctg catatttaaa
     1561 gtcaatttca tgttgaccag gggcaacttc atggtgacta gcttcaatat cgaagcccat
     1621 atcctctaat tctaaaacaa tatcacgacg acagttttca cctaaatctg taggtgctaa
     1681 atcgaaatat ccaccatcat cattaagttc taaagttggt tccccttttt catccaactt
     1741 aaacaagaag aattctggtt caggccctag gttaaagtct gtgaagccta aatcttccat
     1801 ttcttttaat acacgtttta agtttgcacg aggatcccct tcaaatggtg ttccatctgt
     1861 tttatataca tcacaaatta aacgtgcaac tttaccttgt ccagcagtcc atgggaagat
     1921 tacccaagta tctaaatctg gatgtaagta catatctgat tcttcgatac gtacgaaacc
     1981 ttcgatagaa gaaccgtcaa acatcatttc gttatcaagt actttttcta attggcttac
     2041 aggcacttca acatttttaa ttgttcctaa aatatcagtg aattgtaatc ttaaatatct
     2101 tacattttcc tcttctgcaa atttacgaat gtcgtcttta gtgaaagtac gttttggcat
     2161 tttaaaatcc tccaaatttt atttaataaa tcgggataaa tcaccacgat ttattggcaa
     2221 agtttctcca ataggctttt gcgtggcatc tacaatcatc ttttttctta tctcttgttc
     2281 atctgttgtt aaatgctctt gtgagtcata aatgatttgt ttaatccctt tgatattaaa
     2341 tcctttttct aataatgatt taatttctag taatctttcc aaatcattga gtgagaacag
     2401 acgtttttga ccttctgttc tttcaggttt gatgagttca tgtgtttcat agtaacgtat
     2461 ttgccttggc gttaaatccg ttaacttact tactacactc atagagaaga cagccatatt
     2521 tcgtctgatt gcatcattcg atatcattgt tcctctcctc tacttttgaa cttgttaata
     2581 acttatcata atttttttag gatttcaaaa atatgtcagg ttttctgaca taaagctcaa
     2641 aacattgata taactgcaat tttgaccttt aaaacgccat aaaacaatct acagctaaaa
     2701 tattttttca atttttaaaa ataatctgat aaatctccgc aaaaaaattt cacccaaaac
     2761 acattatcat ttagggtgaa atttaatcta ttatataagt ccttgttctt tcaactgatt
     2821 aacagctctt gtcacagcaa ttttaacgtg ttcatatgtt aatcctcctt gaacatatgc
     2881 ttcataagga ggacgaatag gtccatctgc agataattca atcgatgaac cttgaataaa
     2941 cgtaccagca gccataataa catcatcttc gtaaccaggc atataactcg gttctggact
     3001 aaaatgtgca ttaattgggg atgcgtgttg aatactttga caaaatgaaa tcatttgttc
     3061 tttcgtttta aatttaactg tttgaattaa atcagttcgt ttttcgtagt acttcggcgt
     3121 tgtgttcata ttcatttttt ctaaaaataa actagtaaac aatgcccctt taagactctg
     3181 actgacaaca tgtggtgcta aaaagaaacc ttgatacatt tcaagcaatg catttaatga
     3241 tgcacctgct tctttaccaa taccaggtgc tgtcaatcta taaccacatc gttcaattaa
     3301 atcttttcta ccagcaatgt atccaccaat cttagctaaa ccaccgccag ggtttttaat
     3361 taatgatcct gctattaaat cggcaccaca ttctataggt tcacgtcttt caacaaattc
     3421 cccataacag ttatccacaa atattaaaat attaggatgc acttctttca acctagtaat
     3481 cacctgttca atttcatcta gaggaattga aggtctttga tcatagcctt tcgaacgttg
     3541 aatcgctatt actttggtac gctcagaaac cccatctaac acactttcaa tatcgatctt
     3601 accttcttta agtgcaatat ctttatacga tacaccgtgc tccattaaac tttcaatacc
     3661 atttccgttt acaccaatga cttcaagtaa agtgtcatat ggacttcccg ttatataaat
     3721 taattcatca ccatgtttta ataaactttg taatgcaata gtaatcgcat gcgtacctga
     3781 aataatttgc ggacgaacaa ttgcatcttc tgctttaaat gcctgcgcat atatttcttc
     3841 taaatgatca cgtccaaaat catcatatcc gtatcctgtt gatccttgta gatcactttc
     3901 ggttgctttg acatgatgaa atgcatttaa tactttttct tgattaatat atgctgtttc
     3961 ttcaatttct ttaaaatatg gtgctaacgt tgattcgacg tcagctacta tcttacttat
     4021 atctttcatt tgaatttcgt ccttttatta ttttttaaat ccttttatac gataaacatt
     4081 ttcaatttca tcatatttaa gttcagtcac taatgtatgt tgttttagaa aatataatcg
     4141 atctgcatta ttcgtcgcaa ttgtctcatc ataataagtt aattgccttt tgatttcatc
     4201 aataaataac gtcttaacaa gtaatttatc accatcattt ttagaagaaa caaaaacatt
     4261 aggcaaatca cttgctggac aatttgatgc atgatcacat aagtcctttt tattaaaaat
     4321 aactatttga gaaatatgac tcatatctaa ttgtttgatt aaatcattaa ctgtgtcata
     4381 ctgcgtacgg tattcaggat ggctactatc tacgacatgt actaataaat ctgcaccttt
     4441 agcctcttct aaagttgatt taaaagctgc aattaacgtc gtaggtagtt tctgtataaa
     4501 accaacagta tctgaaataa ttaaattaaa tccatcattt atttgaattt gtcgtgtttt
     4561 aggatctaac gttgcaaata attgatcttt ttcatatgtc tcttcatttg ctaaaacatt
     4621 aaaccatgat gatttaccag catttgtata accaactaaa gctacttgaa acacctgatt
     4681 ttgatttctt ttatttcgat atctttcgcg atgttcttct accgtccgca attgatgttt
     4741 aatttcattc atacgagttc gaatatgtct gcgatccatc tctaacttcg tttcaccagg
     4801 gcctctagtt ccaataccgc cacctaaacg agaaagactt ttaccatggc cttgcaatct
     4861 aggtaataaa taatcaagtt gtgctagctc tacttgcaat ttaccttctt tacttcttgc
     4921 tcttaatgca aatatttcaa gaatcaactg agttctatca ataattttta cacctaaagc
     4981 ttcatttagt gatttggatt gtgcagtcgt taattcatca ttcgtgatga ctacatcaat
     5041 atctttgaac tcaataaatg cttgaatttc ttccatttta cctttaccaa cataatattt
     5101 gcgatctaca cgatctctgt tttgagtaat ttgacccaac acttcaagtt ggcaagtctc
     5161 tgataaagat gataattcct ccattgtaga ctcaaaatta aattgcttat catcttgagc
     5221 atgtacaccg actaagacag ctttttctag tttattttta gtatcatgaa tttgttgctg
     5281 agccatgtta tacatctcct taataaaatc ctactcaaag tattctatca tagagtttta
     5341 gtaaagacta ttacacgatt ttatggtaca ttttaagtaa atcgaaagga tgattcaatg
     5401 gagacaattt atgattttgt agtagaaaca aataaaggtg ttacttacaa attagatgca
     5461 tataagggtg acgttatgct aattgttaat acagctagcg aatgtggctt tacttcacaa
     5521 tttgaaggtt tgcaatcatt atacgagaaa tacaaagatc aagggtttgt aattctaggt
     5581 ttcccttgta atcaatttgg tggccaagaa cctggatcag gcgaagaagc agctcaaaac
     5641 tgtaaattaa actatggtgt taccttccct atgcatcaaa aaatagacgt aaaaggggaa
     5701 catcaattac ctttatttag atatttgact gcggcacaac acggattctt taatgagaaa
     5761 atcaaatgga atttcactaa gtttttagtg gatcgcgaag gtaacgttgt taaacgattt
     5821 gcacctcaaa aaaaacctgt tcaaatcgaa agagaaatag aaaaattatt ataaaatata
     5881 ctagctcata gcaaataaaa cgcttcatat aaagtcgtat catatattga ctcgaccttt
     5941 atatgaagcg tttttattac aaacattcct ctattgtatc ttgtcatttg aacatcattt
     6001 aattgatgtc acatatgact ttatttctat atctatttat catacttcaa acgttatgta
     6061 cacgagtcat taaatcgtgc ccatatgatt acaacttatt cttcactttc agtagatgct
     6121 tgaccttcag tttctactgt ataagtgctg atcgcatgtt tgtaaatcaa gtgttgtttg
     6181 ccttgagaat ttaagcttac gacatacttg tgtattcttc aataacacct ttcatttgga
     6241 aaccgtttag aaagaataca gttacttcag tttggtttgc tttaaaattc tctagtgctt
     6301 tgtcttggat gttttcgttt gcaatcatct gtcggactcc ttttacttaa tctgggttgt
     6361 aatctcatct aacatcattt gaagtgacat attttcttta tctaaccaat gaacactcat
     6421 tttattcttg aaccatgtca tttgtcgttt tgcatattgg cgtgaatgtt gctttaaatc
     6481 attgacagca tcttcataaa tcatttgtcc gttaatcaca ggtattaatt ctttatatcc
     6541 aatagcttgc atactttggc aagattcata gccttgttca acaagttgtt gcacttctct
     6601 aaataatccg tgatccaaca taatatcaac acgtttattt attcttgaat ataatgtttt
     6661 acgcgacatt tcaatcccta ataataatgt atcataattt tcagtaaatt gttgcacttt
     6721 cttgcgatta ctcaaaagtt tttttgtttt taaataatat tcaatagcgc gcaaccctct
     6781 ttggcggttg ttagggtgaa tattttctgc agaaaccgca tcaaattgag ctaaataatc
     6841 gtgtagttgc tgattatcta aatgttctaa tgcagataac ttttgtttaa ctatggataa
     6901 ttgtgcaggt gtaactgttt catcttctaa ttcataatta tatattaatg attgaatata
     6961 taagcctgtt ccacctgcta tgattggaac tttacctcta ttcgttatat cagtaattaa
     7021 atcttctgct aatcgcttga attcatatgc tgaaaatgta tcatcaggat tcaagatatc
     7081 aattaaatga tgtggaatac catccatttc ttcaggtgtt acttttgcag ttccaatatt
     7141 catatgtttg taaacttgca tagaatcacc gcttatgatt tcaccattga tacgcttcgc
     7201 gagttctatg ctaagctctg ttttacctga agcagttggc cccacaatta ctacaataaa
     7261 aggcttattt ttattcatat ttaattccac tttctttatt ttttataaaa ttgattcaac
     7321 catttaaaca agtcttccca aattttgtca tgatcttttt caaataacac ttcatgacgt
     7381 ttatgatgat aaagattcac tttaacattt ttaatgccag cttttctata tatattcgcc
     7441 aattttaaaa tccctttacc ataatcacct aaaggatcgt catatcctga aactaataat
     7501 ataggcatgg catgatttaa cattttcata ttttttaatt gtgatgtatg tagcatataa
     7561 taggctgttt gatataataa ttgatttgac acattaaatc cactatatgg atctttaata
     7621 aagttatcta cttcaattgg attactagaa atccaatcac tttgtgtacg taatggacgt
     7681 atttttttat taaaactatt tgatactaac tggttaaccc attcaactcg tttctcagca
     7741 ccataaattt ttgtaatcag ttgtaacact ttaacggttg gtaaaccttt ccataatgaa
     7801 tacataccag taccacttaa aattagacca tcaacatatt gcggatatgt ttctacaaac
     7861 aatctagcta taacggatcc cattgaatgt ccgattataa tgtatggttt atcaacattg
     7921 cctctcactg tttgcgctac ttcaaaggca tcaccgataa ctcgtttcat atcatcgtaa
     7981 tgccctcttg ttgattcatc aatattaata ccatgtcctc gatgattatg acgtatcaca
     8041 tcgaagccat gctttgaaag tgcatgtgct aatttatcgt aacgttccat atgttcagcc
     8101 ataccatgaa atatatgaat aattcctttt gaatctacat ctgtaaaatt cactttaact
     8161 tcaatattcg tattatcttg tacagttaat ttaaattgtt tttcagtcat catctcacac
     8221 ctttaataac aacgctttta tattcgcttt tattattatc atacatttag tgatgcatag
     8281 tttcaattga ctaatgtatc tcgtataaaa aaactgatgc ccttaaaaca ggacaccagt
     8341 ttatgattgt acaaattaat tattctttaa ctgctggttg attatttcca tgttgcgctt
     8401 ctttaattgc ttgctcaact tcttcagtat atgcttcaat ttgagctgga gagtaatcaa
     8461 gcatatctgc cataatatcg atgacagcat ctttataatc taatacatct ttaatattga
     8521 aatacatttt accagaacga cgaactaaga aatcgttagg tttgtatacc atttcttgtt
     8581 gaatactata aacaagttct acataaattt ctaatggtaa cttgctatca tggtattgag
     8641 atgtttgcgc aatgttgaac aattcatcaa catttgaacc atatttagat gctaaacgtc
     8701 ttgcaacatc ttcatcaatg ccgaatcctt tagctacatc tactttttgc tctacaaacg
     8761 catcaaagtt cttgctacca cctacatcgc cacctgaaat tgccagacct tttgtattac
     8821 atggactaaa tgttaaaccg tagtcttttt tcaagcgttt agatactaaa tcaacaatgt
     8881 cttgagccat gtgacgatag cctgttaatt taccacctgc aatagttaat aaacctgatt
     8941 taccttccca aatttcatcc ttacgagaga tttcagaagg gtctttgcct tcttcgtaaa
     9001 ttaatggtct aattcctgcc catgttgatt caatatcttc atctgtaaca ttaacactag
     9061 ggaacatgta attaatcgca tcgattaaat agtctctgtc ttcttgtgta gttaatggtg
     9121 aagatttgat attgtcatag aatgtatctg tagtacctac atacgctttt ccttcacgtg
     9181 gaattgcaaa aatcattctt ccatcttttt cagtatcaaa gtatactgct tgacctaatg
     9241 ggaattttga ttgatcaata acaacatgta cacctttagt taaacgtaat tttttattat
     9301 tgcgtgcata atcaccactt ctaacatcat caacccatgg acctgctgca ttaaccactt
     9361 ttttagcctt aattgtataa ttttcattag ttaatttatc tataacttta acaccattta
     9421 cttgttgatt tttatcataa gtgaagtgtt cagatttagt atagttgata atttctgcgc
     9481 ctttttcagc agcacgcttc ataacttcaa tagttaaacg cgcatcgtca gtacgatatt
     9541 caacatagta accgccgcct tttagacctt cttttttaac taatggttct ttagctaaag
     9601 tttctttttt agataacatt tttttacgtt cagacttctt aacacctgct aaacgatcat
     9661 acattcctaa accaattgat gttgagaatt taccaaatgt tccaccttta tgcattggta
     9721 aaagcatcca ctctggagtc gtaacatgag gtccattttc ataaacaatc gcacgttctt
     9781 taccagtttc ggcaactact ccaatttgga attgttttaa gtaacgtaaa ccaccatgga
     9841 ctaattttgt agatcttgag cttgttcctt gtgcaaagtc ttgcatttca actaatgcaa
     9901 ctttcattcc tctttcactc gcgtctagtg caatacctgc acctgtaata ccgccaccaa
     9961 taattactaa atcatattca tcatttctta aattcttttt aatatgttct ctcttaaaag
    10021 tagacaacgc cattccaaaa cgcctcctaa aaaatgtaaa aacgagagac ttgtcccaca
    10081 taaacaatcc ttcaattgta catgttcgaa caaatctctc gcttctcgat tcaaatatta
    10141 acttaaattg attgtatcac aatgtttata caaaagtcta atctacaagt ttattctgtt
    10201 ttaaaaactt gtgttgcttc aacagctttt ttccaacctc tatataattt ttctctttcg
    10261 ccttcatcca ttttcggatc gaatttttct tctaatttcc agtttttagc gatatcatct
    10321 ttactctccc agaatccaac tgctaatcct gctaaaaatg cagcacctaa agctgtagtt
    10381 tcttgaattt caggtctttc aacagaagta ttaacaatgt ctgcttggaa ctgcataata
    10441 aagttgtttt taactgcacc accatcgaca cgtaaacttt ggacatcaat gccagagtct
    10501 tttgacattg cttccataac gtcacgagtt tggtaacata gtgattctat agttgcacgg
    10561 ataaagtgct ctttttcagt tccacgtgat aaaccgaaaa tcgcaccacg tgcttcagaa
    10621 tcccaatatg gtgttcctaa acctacaaaa gctggaacaa cataaacacc ctcagtagag
    10681 tcaactcgtg tcgcataact ttctgattgt ggtgctgaat taatcattct taatccatca
    10741 cgtaaccatt ggattgctga acccgaaaca aagatggaac cttcaagcgc ataatttact
    10801 tttccatcaa taccataagc aattgttgtt aataaaccac tttcagattt aaccgctttg
    10861 tcacctgtat tcattaacat gaagccacca gttccatatg tgtttttcac gtcaccacgt
    10921 tcgaagcaag cttgtccaaa taatgctgct tgttgatcac cagctacacc agcgattggt
    10981 acttcttgac catagaagtg gtaatcaatt gtcttaccat atacttcact tgaaggttta
    11041 acttctggca acatattttt aggtactgta agtagttcta ataactcatc gtcccattct
    11101 aaatcatgga tattaaacat taatgtacga ctcgcatttg aataatcagt aatatgcgca
    11161 gcttttcctg ataatttcca tactaaccaa gtatcaatcg ttccaaataa tagatcgcca
    11221 ttttctgctt tttctcgtgc accttcaaca ttgtctagaa tccatttaac ttttgtacct
    11281 gcaaaatacg gatctaaaag taatcctgtc ttatctctaa atgtttgttc atatccttgt
    11341 tgttttaatt ctgaacaaat tgattgtgtt tgacgtgatt gccaaacaat tgcgtgataa
    11401 attgggcggc cagtatgttt gtcccaaacc accgttgttt cgcgttggtt tgtaataccg
    11461 atacctgcaa tttgatcagc tctaacatca ttttcattaa ttacttccgt cattacagct
    11521 aacacagatg tccaaatttc atttgcatca tgttcaaccc aacctgattg tggaaaatat
    11581 tgcttaaact cacgttgtgc tacccctgca atttcccctt tttgattgaa taaaatcgct
    11641 cttgagcttg ttgttccttg gtctatagat aaaatatatt tttccattag tagtatctcc
    11701 tttattaaaa aataatcgct aacccattgt acaaaatctg ttaaattttg gcgaaaaaat
    11761 agcataatcc atgaatcaaa catgttaaat tatgctattt acgtttcttt ttattttagt
    11821 aaattgattc gatatcacct ttttttgatg atttatttaa aatgtaacct aaaatcaaag
    11881 taataataac tacaacaatt gcaattgcac aaccaatatt aaatgtatgt ttataaaata
    11941 cagcgtatac cactgcacct aataaaccac cggcaattgg tcctaagata ggaacgattg
    12001 catatgacca atttgaacca cctttaccag ctattggtaa aatcgcatgt gcaattctcg
    12061 gacctaaatc acgtgctggg ttgattgcat aaccagtagc accgcctaaa cttaatccga
    12121 ttgcaacaat taatgctccg acaattaaag gatttaaacc atcggcaatt ttgtttacac
    12181 cgataaataa aatacctaaa gttaatgcca ttgttccgat aatctcactt aaaaagttgg
    12241 caaagtaatt cttaatagcc ggtgctgtag agaaaacacc taatttcgcg ccagcttctt
    12301 ctgtcgcttt ccaatgtggc aagtacatta accatacaat tgttgctccg acaattgcac
    12361 ctaacatttg agcaacaata taaccaggaa ctaatgacca atcaaaactt ccgtctaatg
    12421 caagagctaa agacaccgct gggtttaaat gtgcacctga gaattgaccg acagcaaaca
    12481 cacccattgt aaccgctaat ccccatccag ctgtgatgac aatccaatca gcaccattcg
    12541 cagcacttct ctttaaattg acattggcac aaacgccacc accaaaaagg attaagattg
    12601 cagttcctag gaattctgct aaatatacat tcatattaaa tgtcctccca aaaagacaaa
    12661 gagattccta cactagcagt ttggctcgta gaaatctccc gtctctattc tatgtattaa
    12721 cttttattat atttgttatg atactatcga tatgaaagcg ttgtcaatgg tttttgtaaa
    12781 aattttgtca aatttaattt ttcaagtctt tataaaagta gtttaatttg aaaactagaa
    12841 atatcaatcc gcactaattt atttatgacg ccattatgtt taattgacat tcttcagcca
    12901 tattttttat gactagttgc attttttact aataaacacc tct
//
LOCUS       DAMZLE010000067        12900 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMZLE010000067 DAMZLE010000000
VERSION     DAMZLE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.067"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            351..1310
                     /locus_tag="POB92_001648"
     CDS             351..1310
                     /locus_tag="POB92_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6579148.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSSKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLLKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1356..1487)
                     /locus_tag="POB92_001649"
     CDS             complement(1356..1487)
                     /locus_tag="POB92_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579149.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1551..1766)
                     /locus_tag="POB92_001650"
     CDS             complement(1551..1766)
                     /locus_tag="POB92_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDI6579150.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1931..2482
                     /locus_tag="POB92_001651"
     CDS             1931..2482
                     /locus_tag="POB92_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579151.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVESKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESSTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2533..3471)
                     /locus_tag="POB92_001652"
     CDS             complement(2533..3471)
                     /locus_tag="POB92_001652"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI6579152.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            3653..5014
                     /locus_tag="POB92_001653"
     CDS             3653..5014
                     /locus_tag="POB92_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDI6579153.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            5001..6674
                     /gene="menD"
                     /locus_tag="POB92_001654"
     CDS             5001..6674
                     /gene="menD"
                     /locus_tag="POB92_001654"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDI6579154.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTINRVSWLEKWQRLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHGTL"
     gene            6661..7464
                     /gene="menH"
                     /locus_tag="POB92_001655"
     CDS             6661..7464
                     /gene="menH"
                     /locus_tag="POB92_001655"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDI6579155.1"
                     /translation="MAHYNYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            7457..8278
                     /gene="menB"
                     /locus_tag="POB92_001656"
     CDS             7457..8278
                     /gene="menB"
                     /locus_tag="POB92_001656"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI6579156.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVSE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(8516..8845)
                     /gene="sspC"
                     /locus_tag="POB92_001657"
     CDS             complement(8516..8845)
                     /gene="sspC"
                     /locus_tag="POB92_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDI6579157.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(8883..10064)
                     /gene="sspB"
                     /locus_tag="POB92_001658"
     CDS             complement(8883..10064)
                     /gene="sspB"
                     /locus_tag="POB92_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDI6579158.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSSKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(10146..11147)
                     /gene="sspA"
                     /locus_tag="POB92_001659"
     CDS             complement(10146..11147)
                     /gene="sspA"
                     /locus_tag="POB92_001659"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDI6579159.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQSQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDNPNNPNNPDNPDNGDNNNSDNPDAA"
     gene            complement(11675..12829)
                     /locus_tag="POB92_001660"
     CDS             complement(11675..12829)
                     /locus_tag="POB92_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6579160.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT         4483 a         1830 c         2326 g         4261 t
ORIGIN      
        1 aagacgttta agtcatcagt tacattttgt atttataaat aaagattagc acagcatagt
       61 acaaagttta gaggtaaatg aattaattat tgcatcgcta tttatgaatc gtaaacacta
      121 ttatgaaatt gcaagagtag tatggctaat tagtatgatg gtttgagata aatgcgtgtt
      181 attaaccagt gagtcatctt caaaattaga ggccatttgc tgtttataat aagatgattt
      241 gataatcaac tatgataaga aaaaataata acctcttaaa tatatcataa acaaattaaa
      301 tgataaaata ataatatagt tttctactaa agttgtttgg aggtatatgt gtgaatagga
      361 atatcgttaa actagttgtg tttatgctaa tcttagttgt agcagtagcg ggttgtggtc
      421 aaaaagatac tgaagagaaa actgaaatga cgacaataaa agatgaatta ggaactgaaa
      481 aaattaagaa aaatcctaaa cgtgttgttg tattagaata tagttttgct gattatttag
      541 cagcattaga tatgaaacct gttggtattg cagatgatgg cagcagtaaa aatataacaa
      601 agtcagtaag agataagatt ggggcatatg aatcggttgg atctagaccg caaccgaata
      661 tggaagtgat aagtaaatta aaaccggatt tgatcattgc agatgttagc agacataaga
      721 aaatcaaatc agaattgagc aaaattgcgc cgacaattat gttagtcagt ggtacgggag
      781 attacaatgc aaatattgaa gcatttaaaa cagtcgctaa agcagttggc aaagagaaag
      841 aaggcgagaa gcgtctggaa aagcatgata aaatattagc ggagattaga aagaaaattg
      901 aacagagtac gttaaaatct gcatttgcat tcggtatctc aagagcaggt atgtttatta
      961 ataatgaaga tacatttatg ggacaattct tacttaaaat gggtattcaa cctgaagtca
     1021 caaaagacaa aactacgcat gttggtgaac gcaagggtgg tccttatata tatttaaata
     1081 atgaagaact tgccaatatc aatccaaaag ttatgatttt agccactgac gggaaaacgg
     1141 acaaaaatag aacgaaattc attgatcctg cagtttggaa atcattaaaa gctgtgaaag
     1201 ataacaaagt ttatgacgtt gaccgaaata agtggttgaa atcaaggggg attatcgcaa
     1261 gtgaaagtat ggcagaagat ttagaaaaaa ttgcagaaaa agcaaaataa aaatacagcg
     1321 ctactcgtaa atcatataag agtggcgctg tatttttaat tatgtttatt atctgtcgga
     1381 tgtgatgatt tacctgaaag tttatttcga ataaatttaa ttacataacc gacaaggatt
     1441 gttttaacaa ttcttttaat gaattggcgc atcgttacat acctcatttc tctatatctt
     1501 atgaactata tacccattca tatatgcttt ttaaacgtca ttgtcactat ttaattttta
     1561 gggaatataa tataaccatc tttatctgct tttttagtaa aaatgacaaa aatggcatgt
     1621 attattgaga tgatggtagg gatacctgtc cagaaaaata ataagtgaaa aagaccttgt
     1681 ccaaatttat cagcataaaa tttatgaata cctaaacctc caagaaataa tgcaacaata
     1741 acataaatgg ctttattgac tttcatttgt aaatcctcct taactataat tctacttaaa
     1801 ttcgttgtga aaaccaatat ttttaacttt agaattttca aactttctaa aattataagt
     1861 atatcttttt aaaataagct agaatttcta tataataaat gttaataacg taaaagggaa
     1921 tgatgacata gtgatacgtc aagcacgtcc agaggaccga tttgatattg cgaagttagt
     1981 ttatatggtg tgggatgata tggaattaga attggtaaag catctaccta aagacatggt
     2041 attagatgca attgaaaaaa gctgtgttga tgcaacatat cgaacttttt atcagcatat
     2101 tttagtttat gaagtagaaa gtaaagtagc aggttgtatt attagctata gcggtgaaaa
     2161 tgaattgaaa tatgaaaaag catgggaact acttgacttg ccagaagaaa taaaacaata
     2221 tggcacgcca ttacctgtaa aagaagctaa agacgatgag tattatatag aaacaattgc
     2281 gacatttgaa gcatatagag gtagaggcat cgcgacaaag ttattaacgt cattacttga
     2341 atcaagtaca catgttaaat ggagtttgaa ttgcgatatt aataatgaag cagcattaaa
     2401 gttatataaa aaagtaggct ttatatctga tggacagatt gaattataca agcacatgta
     2461 tcatcattta attgttaaat aaaaaactcg acagttcgat gtaagtcgat tgccgagtag
     2521 tatcatttta tattaaatgc ctgcaaataa tgcactaata taaataccta atgcatataa
     2581 taaaccgaaa aatgtatttg ttttaccagc agcagccatt gctggcatca ttgtaggcgg
     2641 tgtatcattc ttcttgaaac gtctgataac tttaacaggc attgggaatg ataacaacgc
     2701 aagtaagtaa aataatgagc caccaggttt aataatgatc gtaagtacaa taaaggcata
     2761 agcgataaag tacatgattg ccataaatgt taaagaagca tttttaccta atagaatggg
     2821 taaagttttg cgaccacttg ctttatcttt gacacggtcg cgaatattgt tagccatatt
     2881 aattaaaccg atagtgatta ctataggtac acttaaccaa attacataac tttgaatatt
     2941 gccagtttga ataaagaatg caataacgat aataaacata cccataaata cgcctgagaa
     3001 taattcaccg aaaggcgtcc atgaaatagg gaaaggacca cctgtatata ggtaaccaac
     3061 agccatacat actaatccaa ctggtaataa ccaaaatgaa gagttagcag ctaaaaacaa
     3121 acctaatatt gctgctaaga tgtaaaatgc aatggctaat cgtagcacaa gctctgggct
     3181 cataccgttg cgaacaatgg caccaccaat gcctacagat tcatgatcat caaggccttt
     3241 tttataatca tagtattcat taaacatatt agttgctgct tgaataagta agcatgctag
     3301 taacatggca atgaataggc tgattttaat atgatcttcg ctaccaagaa aatatatttt
     3361 agatgctgct gtaccaacta aaacgggtac tacggaagca gttaatgtat gaggacgcat
     3421 taaatgccaa tatttcttaa ctgtagaata ttgctgatat tgattactca ttgaatacaa
     3481 aacctcttta aatcatattt tttataacaa agatatttta atagaaagta gcataaaggt
     3541 caattgacgt gaaaaattgc aagataagtt taagcaattc ttgtaacaca attatccaaa
     3601 aatgatacaa tataataatg agtgaaaaaa gacgaagaaa gaagtgaaat ggatggctac
     3661 gggcgtatta gaggacgata ttgtcaaaga gatatatggc agctcaaagg aatgggtttc
     3721 agttgaagtg aaattatcac agtcactaga cccgagcaca ttatttcatc tcactgacaa
     3781 tgaggcagga gatcgctttt atatgcgttt gaatgataat cgaacgtcat attttggcta
     3841 caaagcaatt caattattca aaaataattc taaaaataaa caatctattt ttaaagactg
     3901 ggaaaaatta aaacataaca tcacatttat acatccgcaa tctgagaaac atcatcttcg
     3961 agttgttgga ggattccaat tttcgagtca taaatcagat gatgaatggc gagagtttgg
     4021 actaaatcat tttgtattac ctgaagtttt aatttcaact gataataatg ggacattttt
     4081 aacttataca gttaaaaggg aaagttttac tgttgaggca ttgaacgatt taatggattt
     4141 gttcaacaat atatcggaca tagatgtgga cgagcaaatt ggggaaatta ctagaaatga
     4201 agatatttat aaagatgact ggcgtcaact tgtagtagaa gctatagaat ctattaataa
     4261 tgaagaaaaa attgtactag cacgtagacg gttaataaag ttcgataaag atatcagtat
     4321 tccatatatt ctaaagcaag catattctaa agaaaaaaac agttatatat tcttgttaga
     4381 atcacaagat tctatattct tttcacaaac acctgaacaa ttaataaagg tcaataataa
     4441 aatactatcg actaaagctg tagcaggtac aattaaacgt tcacaagatg aggacgaaga
     4501 tacaaaaaat gttgaagcat ttttaaaaga taataaaaac ttaatcgaac atcgatttgt
     4561 tgttgacagt attttacatg atattaaacc ttatatcact gaattacatt atgataagac
     4621 gcctaaaatt ctaaaaaatg atcatttata tcacttgtac actgaaataa aggcgccact
     4681 gaaggatgat tcgtatatta gtttaattga taatttacat ccaacacctg ctttaggtgg
     4741 ctatccaaaa gaatttgcga tggattttat tgaacagaaa gaatttggta cacgaggatt
     4801 atatggtgcg ccggttggct atatagatat atatgatgat tgtgaattta ttgttgcaat
     4861 tcgttcgatg cttattaaga aagcacaagc aactttattt gctgggtgtg gcattgttaa
     4921 agattctgat ccagatagtg aattggcaga aacgaacctt aagttcacac ctatgatgaa
     4981 tgcattagga gtcgatatga atgggaaatc ataaagcagc tttaacgaag caagttttta
     5041 catttgcatc tgagttatat gcgtacggcg taagggaagt agttatcagt ccgggatcac
     5101 gctcaacgcc acttgcactt gcatttgaag cacatccaaa tattaaaaca tggatacacc
     5161 ccgatgagcg aagtgcagca ttttttgcag ttgggttaat taaaggtagt gaaagacctg
     5221 tcgctatatt atgtacgtca ggtacagcag cagcgaatta tacgcctgca attgctgaaa
     5281 gccaaattag tagaattcca ttaatcgttt taacaagtga ccgtccgcat gaattaagaa
     5341 gtgtaggcgc accacaagcg attaatcaag taaatatgtt taataattat gtaagttatg
     5401 agttcgatat gcctattgcg gatgatagta aagagaccat taatgcaatt tattatcaaa
     5461 tgcaaattgc tagtcaatat ttatatggac cacataaagg gccaattcat tttaacttgc
     5521 catttagaga tccgttaaca cctgatttga atgcaacaga attgttaact tctgagatga
     5581 agattttacc gcactatcaa aaaagtatag atgcatcggc attaagacac attttaaata
     5641 agaaaaaagg tttaattatt gtaggggata tgcagcacca agaagttgat caaatactaa
     5701 cgtattcaac gatatatgat ttgcctattt tagctgatcc tttaagtcat ttaagaaaat
     5761 ttgatcatcc gaatgttatc tgtacatatg atttgctgtt tagaagcggc ttagacttaa
     5821 atgtggattt cgtaattcgt gttgggaaac cagtgatttc taaaaagttg aatcaatggt
     5881 taaagaaaac tgatgcattt caaatattag tgcaaaacaa tgataagatt gatgtctttc
     5941 cgatagcacc agatatttca tatgagattt ctgcgaatga tttctttagg tcattaatgg
     6001 aagacacgac catcaatcgc gtaagttggt tagaaaaatg gcaacgctta gagaaaaaag
     6061 ggcgtaaaga aattaaatgt tatttggaac aagctacaga tgagagtgca ttcgttggtg
     6121 aattgattaa gaaaacatct gaaaaagatg cattatttat tagtaatagt atgcctatca
     6181 gagatgtaga taacttgtta ttgaataaaa atatagatgt ctatgcgaat cgtggtgcga
     6241 atggtattga tggtatcgtt tcaactgcac tgggtatggc tgtgcataaa cgaataacat
     6301 tattgatagg tgatttatca ttttatcatg atatgaatgg actattaatg tcaaaattaa
     6361 ataatattca gatgaatatt gtattattga acaacgatgg tggcggtatt ttttcatatt
     6421 taccacaaaa agaaagtgca actgactatt ttgaacggtt gtttggcaca ccgacgggat
     6481 tggatttcga gtatacagct aagttatatc aattcgattt taaacgtttt aacagtgttt
     6541 cagaatttaa aaatgccaca ttgttatctg aaacttcgac gatttatgaa ttgataacga
     6601 atcgcgaaga taactttaaa cagcatcaaa ttttatatca gaaattgagt gaaatgattc
     6661 atggcacatt ataattacta tgaagcaaac gttgagacca atcaagtttt agtattactg
     6721 catggttttc ttagcgacag tcgtacttat tataatcaca tcgataaata tactgatatc
     6781 tgtcatgtca tcactataga cttaccagga catggcgaag atcagtcttc aatggatgaa
     6841 acgtggaatt ttgattatat tacgacgttg ttagaccgaa ttttagataa atataaagat
     6901 aaatcaataa cattgtttgg atattcaatg ggtggacgtg ttgcattata ttatgcaatt
     6961 aatggtcaca tccctatatc taatttgata ttagaaagta cgtcaccagg tattaaagaa
     7021 gaagcaaatc aattggaacg ccgtcttgtt gatgatgcac gtgctaaagt attagacata
     7081 gcaggtattg aattatttgt taatgattgg gaaaagttgc cattatttca atcgcaacta
     7141 gaattaccag ttgaaataca acatcaaata agacaacaac gattgtccca atcgccacat
     7201 aaaatggcca aagcattaag agattatggt acaggtcaaa tgccaaactt atggccgcgc
     7261 ctgaaagaaa ttaaagtacc aacattaata ttagctggag aatatgatga aaaatttgta
     7321 cagattgcga aaaaaatggc aaatttaatt cctaatagta aatgtaaatt aatttctgct
     7381 acaggtcata caattcatgt ggaagatagt gatgaatttg atacaatgat attaggattt
     7441 ttaaaggagg agcaaaatga ctaacagaca atgggaaaca cttagagaat atgatgaaat
     7501 caaatatgaa ttttatgaag ggattgctaa ggtaacaata aatcgccctg aagtacgcaa
     7561 tgcgtttaca cctaaaacag tttctgaaat gattgacgca ttttcacgtg cacgtgatga
     7621 tcaaaacgtt tcagttatcg tattaactgg tgaaggtgat ttagcattct gttctggtgg
     7681 tgaccagaag aaacgtggac atggtggtta tgtaggtgaa gaccaaatcc ctcgcttaaa
     7741 tgtattagat ttacagcgtt taattcgtat tattccaaaa ccggttatcg cgatggtaaa
     7801 aggttatgct gtaggtggcg gtaatgtact aaatgttgtt tgtgacttaa cgattgctgc
     7861 tgataatgct atttttggac aaactggtcc taaagtaggt tcatttgatg cgggttatgg
     7921 ttcaggatat ttagcacgta tcgttggaca taagaaagca cgtgaaattt ggtacttatg
     7981 tcgtcaatac aatgcacaag aagctttaga tatgggtcta gtaaatacag tggtaccttt
     8041 agagaaagtt gaagatgaaa ctgtgcaatg gtgtaaagag attatgaaac actcaccaac
     8101 agcgttacga ttccttaaag cagctatgaa tgctgacaca gatggtttag ctggtttaca
     8161 acaaatggct ggggatgcaa cactgcttta ttacacaact gatgaagcga aagaaggccg
     8221 tgatgcgttt aaagaaaaac gtgatcctga cttcgatcaa ttccctaaat tcccataagt
     8281 tattttgaat atgatattaa gtcacttgcg tcgtttatta gcgacgatag gtggcttttt
     8341 tattttataa gaatttaaaa ggggctttta attaattatc ataaatataa gatagtttga
     8401 gaaaattaat agagaaaaaa taaatgttaa agattattat tacaatcaaa aaacgagctg
     8461 gtgagaaatt gaattctcgc caacccgctt tatgaagtga aatcatttaa attagttata
     8521 ctaagcgctc ataaacgatt gggcgcgaag tgccaatacc ttgtttggat gacgtttgca
     8581 tgaggatatg ctgtgtatca tcataatcaa gaatataaat gatttcttca ttatcaatag
     8641 atgaaaattt aatgcgttga tgtgccgtat ctataaaaag aatatgatat tgattgtgac
     8701 ttgtatcatc gccatgacgt atacaaattg atttttgtaa ttgatgatta ctccaattac
     8761 caatgaataa attgatattg gtttgttcta gatgtgtgag tttggttgtg tcgtaaacta
     8821 aatttataaa ttgtagttga tacatagttt tacctgcctt taatcaatat ctatattact
     8881 ttttagtaac ctatcattga accataccag ttataatcac gattgaatga taaatgtaat
     8941 aggcttgaat ctgcatcttg gattgataat tctgtatccc aaggattcca gtaaataagt
     9001 ttttcttggt cattaatttt agcattacca acaactgcta gggcatgtcc taaatgtgga
     9061 tcattagggt tttgagatac actttgtgca agaatcataa ttcctacatt atcttttgta
     9121 agttgatcaa cttgttcata tgatggtacg ccttcttgat aatgaatatc tctgccttgt
     9181 gatttaccgt attcaatcat ttgattaggg aatgttgagc aattaggaag gtcttgctca
     9241 cttacttcag ggtataatgt acgcataata tcatgtgcat tataagtgtc tgtattttta
     9301 gttgcattta ataatgctgc catactaaat cctgcacacc atgagttatc gaattgttgt
     9361 tctctaattt tgaagttttt taatgtattt tcatattgaa cttgatcttc ttgtatcgct
     9421 tcgttttctt caactttagt aggtgttact gtatttttta actcttgttt caattttgat
     9481 gaaactgttt tagcactttc tttttctttt acattaccag gtagtggcgt agcttttact
     9541 aatctaactt tgccatcttc ttcaaaataa aatccttttt catcagtaag aacagtgata
     9601 tttgaatttt tatctttaat ttggtctaaa tctttagcga tgaagtttga aattttaaca
     9661 ctgtaattca tgtcttcttt tgatttgttt aaatcatctt tatttttagg gctaagtgtt
     9721 aaagtataaa caattttacc gtcttttata actggataat agtaactatt atcttcttca
     9781 ccattaaatt tataaatttt aaaagcttcg ccaagttcat atttaccagt ttttgcatta
     9841 ctttgtttat ctaatgcttt agcataacca gcaaattgtt gttgtgctag atcttttact
     9901 ttttgaggta ctttgtcact cttaacatta atttctagct gtttagagtg tgaatcggct
     9961 ttggctttat tgttattagc aaatgcgcct agtgataaaa taagcattga aaccattatg
    10021 atgcttataa tattgaatac tctagattta tatgaactat tcatataata ccctccattt
    10081 aaatcaaaat attttgcgtt gctaagactt tgcttctatt tactaaatct aaattaagat
    10141 gaagtttatg cagcgtcagg gttgtctgaa ttattgttat cgccattgtc tggattatca
    10201 gggttgttag ggttgttagg gttatcagga ttgttagggt tatcaggatt gttagggtta
    10261 tcaggattat ctgggttatt aggttggtca tcgttggcaa aatggatatc ttcaatattt
    10321 tgttttaaga agttgcgtac attttcatta ataaataccg caccattaaa ttcatttggt
    10381 acaccgcccc aatgaattcc gatcacttca tttttttcat taaatacagg tgaacctgag
    10441 ttaccaccag ttgtacttaa atcatattgc atagcttcgc ctttgaggta agtgattttt
    10501 cctttacttt cccacattgt tgctacaggt ttatcaccag gatatcctgt tacagtaata
    10561 ttttggttaa cttgtgtttc agcattatta ctcattgttg ctggtttaac tacttcacca
    10621 atatgtttgt tttgctcatt aggggagaat ttaactattg ctaaatcacc ttcgcctgaa
    10681 tatttagtga tttgttcagc agtgaaacca ccatttggat aattgtcttg gttaattgca
    10741 gaagggaatg cttttaaagc atgaggatca ccgtgcgtag catctacgac gtgtttattt
    10801 gttaaaagag tatctttacc tacaactaca ccggaagcaa taaatgtacc agtaggtgct
    10861 tcaacttgaa tataagttac gggtgcataa tgaccattcg ttgtatctgt gatttggtga
    10921 cgatcgttat ttggtaatat aacatttgcg tgttcacgtt gttctaatgg tttaaggtta
    10981 ccgccttttt gaatcttagg tgtttgctgt ttgcttgact gtgattgttg cggatgattg
    11041 tccatagcct ttgaagataa cgcgtttgct gctggagaac tcacaagtgt cgctgttgtc
    11101 aaagttgcaa cgaataaaga actaactttt aaaaatttac ctttcatcta aaaacctcca
    11161 aaaaatttat ttacaagtta aatataacac taaaaatttt taagtcaata agaatatata
    11221 aataaattaa aaagtttttg taaagattag agtttacaaa aacattaagg ggattaaaat
    11281 tgaattgtat tatactttat tcaattgagt taaattgata agatcgtcaa ttgtttgcat
    11341 ttatctaaaa gaataaataa gaaaggaatt gagagcgaat ggaaagtatt tgtttataat
    11401 aataaatagt aacttttgaa taataattgt ttggccaaag gtagattaat atgaatggtg
    11461 atatattatt tgagtgctta agtttcgaag aacaattata agttagcata ttaaaacctt
    11521 ttgatagaat cattttgagt gaggtgttta aaggagagga atataactcg gtagtattta
    11581 cagtctctat agagtattat ttaaaactta tagaatataa aaaactgaac gtactatgaa
    11641 tttagttcac gacgtccagt tcaacatatc atttttattt agtatttaaa tatttagcaa
    11701 gtctcttcat accttctttt aaaacatcca tttcataagc ataggaaatc cttacaaatc
    11761 ctttaccgaa ttctgtaaag gatgagcctg gaacgattgc taaatgtgtt gattcaagta
    11821 aatcgacaca aaattcgaaa tcgtcatcgg tgatatgttt aatacttggg aaaatataaa
    11881 acgcgccttc aggttgagcg gtaatctcaa aacctaattt agttaattca gatactaaat
    11941 aatttcttcg ttctacataa gcttcgttca tatatttagg agcttctaat ccttcgttaa
    12001 gtgctgttat acatgctatt tgagctggaa cattggcaca aatacaatta taggcgtgca
    12061 taaatgttaa tttatcaatc aaatattgag gtcctaatag aaaaccaatt cttattccgg
    12121 ttgctgagtg tgatttactt aaaccaccaa ttaatattaa ttgatcacga atgtcttcaa
    12181 attcagcgaa ggatacatgt ttaccactaa atgtattttc agcataaatc tcatcgctaa
    12241 taataaatat cggatatttt tttaatacat ttacgatatt taaaacttca tttcttttta
    12301 aaactacgcc agttggatta gtcggataat ttaacaagac agcttttgtc tttggagaaa
    12361 tatgactttc taatgcatca ggtgtaattt taaattgtgt tgctgttgta tcaatataaa
    12421 ttggtttacc acctagtact tcgatgagtg gtatgtagcc tgcataaatt ggtcccggta
    12481 ttataatttc atctccaggc tctatgatag aacgtaacgt tgtgtctatt gcttcacttg
    12541 ctccatttgt cacaataatt tcttcaggat cataggaaaa atgataacga tttttgaagt
    12601 attgactaat tgcttcgcga gtttctaata accctttatt gtgagagtat gatgtcttgt
    12661 cattgttaat agcatcaata tatgcctttt tcacaacatc aggcatcggg aagtctggtt
    12721 ggccaatagt taaattaaca caatcatcca aattattcat acgatttgaa aattggcgaa
    12781 tacttggtgc tcttaaatat ttagaattag aatttaaaga aagtttcatt tgtgacacct
    12841 caaatataaa tcaaatattg tctaaaaatt tagaaattaa taatatcata acatactttt
//
LOCUS       DAMZLE010000068        12838 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMZLE010000068 DAMZLE010000000
VERSION     DAMZLE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.068"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(210..1085)
                     /locus_tag="POB92_001661"
                     /pseudo
     CDS             complement(210..1085)
                     /locus_tag="POB92_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365429.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1343..1555)
                     /locus_tag="POB92_001662"
                     /pseudo
     CDS             complement(1343..1555)
                     /locus_tag="POB92_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1624..3162)
                     /locus_tag="POB92_001663"
     CDS             complement(1624..3162)
                     /locus_tag="POB92_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6579161.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLGPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMFIDAPPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDF
                     KVEK"
     gene            complement(3213..4757)
                     /locus_tag="POB92_001664"
     CDS             complement(3213..4757)
                     /locus_tag="POB92_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6579162.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITDWMSKDSDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            4986..5942
                     /locus_tag="POB92_001665"
     CDS             4986..5942
                     /locus_tag="POB92_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6579163.1"
                     /translation="MKKFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6156..6929
                     /gene="phnC"
                     /locus_tag="POB92_001666"
     CDS             6156..6929
                     /gene="phnC"
                     /locus_tag="POB92_001666"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDI6579164.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            6931..7731
                     /gene="phnE"
                     /locus_tag="POB92_001667"
     CDS             6931..7731
                     /gene="phnE"
                     /locus_tag="POB92_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6579165.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7728..8543
                     /gene="phnE"
                     /locus_tag="POB92_001668"
     CDS             7728..8543
                     /gene="phnE"
                     /locus_tag="POB92_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6579166.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAIESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8674..9852)
                     /gene="deoB"
                     /locus_tag="POB92_001669"
     CDS             complement(8674..9852)
                     /gene="deoB"
                     /locus_tag="POB92_001669"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6579167.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(9880..10542)
                     /gene="deoC"
                     /locus_tag="POB92_001670"
     CDS             complement(9880..10542)
                     /gene="deoC"
                     /locus_tag="POB92_001670"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6579168.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKVAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            complement(10623..11975)
                     /gene="tet(38)"
                     /locus_tag="POB92_001671"
     CDS             complement(10623..11975)
                     /gene="tet(38)"
                     /locus_tag="POB92_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6579169.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            complement(11982..12689)
                     /gene="deoD"
                     /locus_tag="POB92_001672"
     CDS             complement(11982..12689)
                     /gene="deoD"
                     /locus_tag="POB92_001672"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6579170.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVGTARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
BASE COUNT         4186 a         2378 c         1997 g         4277 t
ORIGIN      
        1 tttcaatatt attataaaca tttcaactac ttttaaaaac caacaaaaaa atacttattt
       61 taagtagatg agcataagtg aacatgtccc catttttttg caaaattttg caaaaaatga
      121 gtggtttggc ggtgccaaac atgttcaaaa cgtggggaca aaaaagcatg attgcacata
      181 aattgaacat agttctttag ttataataat taattcaacc aaaagtcgat ttgtttttgc
      241 aattggtttt catttcctct taaagatatt ttcattaaat ctgtcaaatc aatagacgct
      301 atatttttca acttatctct atatttattt ttagtacgtc tttctaaatt tccccattcc
      361 tcttcttcgt gagttaataa atgaagcatt gctcgttctt gtatattttc aatcattttt
      421 aaattcggtt ttaaaatatg caaatcatca aaacaatctt tccaacaatc aaccatatct
      481 cgttttaatt caatttccac acgccataga aatgttgaat caatttcaac atctgcatta
      541 tctttacgtt cttgttttta ttataaatcc gaataaacct atcactatta cgcacaccaa
      601 aatattttgt ttctggtttt acattacgtc cataaaatat agttttcttt gccgacttat
      661 ctgacaatgc ataatagtca tttaaatcaa attcaaaatc aaaagccaaa tctaatctcg
      721 taaaactaac atcgtccaaa taactgatga tattttgttt taaccaaagc acttcatcat
      781 gcgaaagctt attaggatta aattcaacgc gcatactacg tctattccaa agagttgctt
      841 ttattttgtc atattcaata taaacttttt ctttaagagc tttagcttta aagtttgttt
      901 gtaaaatatc ccaaagccga acttcaggat tagtactcat aaaatgtgaa agtctctctg
      961 cgttagacat gctaagattc ccaacaatcg ttatagcgtc aaaagacaat tttggaatag
     1021 ctagtgacat cctatgtcaa tttaaccggc tattaccgga tattagagta tccagtttta
     1081 cacatggatg aaacgaaatt caaaacccta aaaaatatgt tccactaaca gcaaaaaaat
     1141 accattatgt tcctactaaa aaactaaaaa tactggagaa caaatgtcag gatataactt
     1201 aggatactat gtaataaaaa tttacaataa aaaaacagga aaacaaattt caagtaaaag
     1261 aatacccata caaagaggat aaaataaaaa acctcgaact gaaatgatga tcttttcagc
     1321 tcgaggttta aatattagtg ccttatttat atagattcgt tatattatat tctctatttt
     1381 cattaacata atccttaaag agttttaaat taatacctgc tagatgattc aaaaatgttt
     1441 catcaacttt taaataattc aataattttt gtggtgtcag taaatttcta tcaaaataca
     1501 actttaataa actattcatt ttgacaggac gtgacatttc aatcacgtcg tctaaagata
     1561 atactttctc gctttaaaca aaaacttacc cgattaaaat caagtaagtt tttcatatat
     1621 ccgtcacttt tcaactttaa aatcaacaac ttgcgggata cgcatcaaat tattatttga
     1681 taaaaaatca ataagtaatt gtgcgccttc aacttgaata tctttcacaa ctggcggcgc
     1741 gtcgataaac atatcatact gaccaccgcc aactgcacga taattattta cacaaattgt
     1801 atatgtctgc tttaaatcaa ctgcgtgacc ttgaatcatc atattgctca cacgttgtcc
     1861 ctttggtctt ccaacatgaa tggtataact tacgccacca tatatatcat aattaaagtg
     1921 ttgtggtttg ggtccaagga agtctgcgct cacactaact tcatcatttt tcacgtcaaa
     1981 atattctgct gatcgttcaa tggcttcttt aagtttggca ccacttacag ctaaaacttt
     2041 aaatgtattt ggaaatgggt aattgttaat aacatctcgc atcgtcacga cttgcttgaa
     2101 accactagca gaatcaaaca aagctgtaca ggcaacatct gcgtcacttt tttctaataa
     2161 agcgtaattc ataaaatttg taaaaggatg cggtgccaca cgtgcctcaa atgcatgatt
     2221 aatcgtcata tcatatggca atgtagtaat ttcgtaatct aaccagtcct ctaactgctt
     2281 tcgtaaatgt tggtcatctt catcaatagt aaatgtggaa tcatctataa caggaagtaa
     2341 ttcacatgat tcaacggata gattttcata ttcatcagta ctcaagacta ctctgcctac
     2401 agttgtacct ctcgtaccag gttgaatcac agccgtttgc ttaaaccttt cagcaatttg
     2461 tcgatgttgg tgacccgtaa taaagatatc tatatcttta gaaaacgctt ctaacatggc
     2521 atatccctca ttttcacccg ttaatacttc ggtcggcgta ccactttcta aatccttttc
     2581 aaatccacca tggtaacaaa ccacaatgat atctgcatgt cgcttcattt caggtaagta
     2641 ttgttgaagt atttcaaaag cactatgaaa cgtaagtgac tgaatatgct ctggttgttc
     2701 ccaatgggga ataaattgtg tcgttaaacc tatcacacca acagtttgat ctccaacctg
     2761 aaaatacttc acaccgttat cagtcaatgt actatcattt tcatatatat tagcgcacaa
     2821 aactggataa ttgagtctgc gtaaagtgtc ttttaagtat ggcaatccat aattaaattc
     2881 atgattacca agcgtaccaa agtcgaatgc cattcgatta taaaaatcaa ctaaaggctg
     2941 gctactgccg ctatgcgcga ttaagtaatt acaaaatggt gacccttgca aaaaatcacc
     3001 attatctatt ttaaaacttt ggtcatactg ccttctgtct tgttctataa catgattcgc
     3061 tagtaacaat cccataggtt gatattgatt tctactcgta aaatctgttg ggaaaatata
     3121 accatgtacg tcactcacga cataaaatgc tatgtttgac atcctcactc actccttcaa
     3181 tcacaaacat ctttcttatt tctattatat atttatttga agtctgttgt aatcaaggtt
     3241 ttgtcaccga gttttaaacg aatctttgaa ccttccatac tttcaagtac tttagcattg
     3301 accttaattg tgacatttcc gttttcatct gctttaactg ttggcaaagt actgtaacct
     3361 ggtgggttat aatcgttatc tttacttgaa aattgtccga tttgacgtcc gccttctttg
     3421 taaataccaa ctctaagatt ttcaagtgtt tgatttgctt caaatccttt taaatgtatt
     3481 gtcatttcaa atggctcatt tacagaaaca ttttgcggga tatcaaatgt tactttttcg
     3541 ttctgatttg gtggcgtatg atcatctggt gtgtttggct gaggatctgc gcctttttcg
     3601 ctgccataac tacctgcttt aaatgttgtt ggatcatacc atttataacc actcggcggt
     3661 tgtgaccatg gctctttttc aggctcagtt gaacgctctg gtcgttcaaa atcaagcaac
     3721 ttagtctttg tatctaatgt tagtccactc gccttaagtg atttcccatc actatcttta
     3781 gacatccaat ccgttatatt atttaatagc ttaccgttgt cttgttcttt aaaaccatca
     3841 tatgttttct tcttttctcc attatcttct cttacatatt tgggcgaact atcttccaca
     3901 agtgatgaat caccgataaa tgctgcttta ccttttccaa ctttagaaat tgctacatag
     3961 gggccttctg ctttaccgcc cccattataa ataccttgat ctacagcatg tgaccattta
     4021 cttttcgctg gcaattgttc tggtgtatac acaatacctt ttgctttctc tggattagta
     4081 attgctaatg ttgatccggc atgcatagag acagatttca caccttcagt aataccgaaa
     4141 ctctcttttg aagaaacaat attgctcgta tttaaatcac ctagtgcatt atatcgaaaa
     4201 cgtacgccaa agtttgtaga taaccaatct gaacttttca caccttgcat tgcagtagaa
     4261 cttttttctt ctgcattcat acctttcgac atatcttcat atgctccacg tcgataacca
     4321 ttcattgcct ctgatgaatc aatacgattt aaatttcggt cagcattgta atgatctgaa
     4381 ataaagacaa cattgccacc ttgtttcaca tagttaacaa ttgctgcctg ttctgattct
     4441 ttgaaaggaa tattagcctc aggaattaca aatattttag aacttttcaa acttgcttct
     4501 gttatgttcg aatgaccatc aatagcttta acgtcataac cttgtttttg tattgaatcc
     4561 gcataatctg aaaatgcacc atcactaacc caatctgcag caccagctgt ttgaccatga
     4621 gaacgatcga ataataccgt tcgctgttgc tttgtaggtt gcgattcatg cgttatagct
     4681 aaagattgcg gtaaagcact taatgatacc gttgcaacaa ttgcagagac agttaatgac
     4741 ttatatattt ttttcatttt gtgaggctcc ttttaaaata aatttcttct tgaattatag
     4801 gataaatata tgtttgaaat gggcaattta acgaaaaatt tacaaaatct tatcaaactc
     4861 ttaaataaag ttattaaaat tcatttttat aaaatacttt ttcgcattta aatgtggtac
     4921 gctataagtg taatttcatt gcatacatat tacacgatta agaatgtgaa ggggacagtt
     4981 atcaaatgaa aaaatttaag tgtttatttg tattaatgtt agcagtcatt gtttttgcag
     5041 cagcatgtgg aaattcaagt tctttagata atcaaaagaa cgctagtaat gattcggatt
     5101 ctaaatcagg aggatacaaa cctaaagaat taacagtaca atttgtacct tcgcaaaatg
     5161 ctggaacatt agaggctaaa gcaaaaccat tagaaaaatt actatctaaa gaattaggga
     5221 ttccagttaa agtgtctgta tcaactaact acaatacaat tgttgaagct atgaagtcta
     5281 aaaaagttga tgttggtttc ttaccaccaa cggcatacac attagcacat gatcaaaaag
     5341 cagctgattt attattacaa gcacaacgtt tcggtgtaaa agaagatggt tcagcaagta
     5401 aagaacttgt agatagttat aagtcagaaa ttcttgttaa aaaagactca aaaattaaaa
     5461 gcttgaaaga tttaaaaggt aagaaaattg ccttacaaga tgtaacatca actgctggat
     5521 atacattccc acttgcgatg ttaaaaaacg aagcaggtat taatgcaact aaagatatga
     5581 aaattgtgaa tgttaaaggt catgaccaag cagttatctc attattaaat ggagatgtag
     5641 atgctgcggc tgtatttaac gatgcacgta atactgtgaa aaaagaccaa ccaaatgtat
     5701 ttaaagacac acgaatttta aaattaacac aagctattcc gaatgacaca atttctgtaa
     5761 gaccagatat ggataaagat ttccaagaaa aattgaaaaa agcttttata gacattgcta
     5821 aatcaaaaga aggtcacaaa attattagcg aagtttattc acatgaagga tacacagaaa
     5881 cgaaagattc aaatttcgac attgtaagag agtacgaaaa attagttaaa gatatgaaat
     5941 aatcattatt taacaaatga atcattagcg aatttggtat taaaagcttt cgttcaatag
     6001 atatattcta gattaatatt gaaaagctag gcgctaaact gaaacagata tagaaaggtg
     6061 tcgctgtaca tttgaaacca tttgtacaca gaaacccaat gtctatgata tttcagttta
     6121 ccttggcttt tctttattaa agaaaggtgt caaacatgag tcaaatcgaa tttaaaaacg
     6181 tcagtaaagt ctatcctaac ggtcatgtag gcttgaaaaa tattaactta aatattgaaa
     6241 aaggtgaatt tgcagtcatt gtcggactat ctggtgctgg gaaatccacg ttattaagat
     6301 ctgtaaatcg tttgcatgat atcacgtcag gtgaaatttt catccaaggt aaatcaatca
     6361 ctaaagccca tggtaaagca ctattagaaa tgcgccgaaa tataggtatg attttccaac
     6421 attttaattt agttaaacgg tcaagtgtat tacgaaatgt actaagtgga cgtgtaggtt
     6481 atcaccctac ttggaaaatg gtattaggtt tattcccaaa agaagacaaa attaaagcaa
     6541 tggatgcact agaacgggtc aacatcttag ataaatataa tcaacgctct gatgaattat
     6601 caggaggtca acaacaacgt atatctattg cacgtgcact atgccaagaa tctgaaatta
     6661 ttcttgcaga tgagccagtt gcttcattag acccattaac tacgaaacag gttatggatg
     6721 atttaagaaa aatcaaccaa gaattaggca tcacaatttt aattaactta cattttgttg
     6781 acttagcaaa agaatatggc acacgcatca ttggtttacg tgatggtgag gttgtctatg
     6841 atggtcctgc atctgaagca acagatgacg tatttagtga aatatatgga cgtacaatta
     6901 aagaagatga aaagctagga gtgaactaac atgcctttag aaacacctac aaagtatgac
     6961 tcccttttaa agaaaaaggt ttctttaaaa acgagtttta ccttcatgtt aatcattgtg
     7021 cttatcattt ggagcttcat ttatacgggg tttaattttg gagatttaat gataggaata
     7081 cctcaaatag gtgatctatt caaacaaatg attccacctg atttcgagta tttacaacaa
     7141 attacaacgc caatgttaga tactattcga atggctatcg taagtacagt attaggtagc
     7201 atcgtttcaa taccaattgc gttattatgt gctagcaata tcgttcatca aaagtggatt
     7261 tcaataccct cgcgttttat tttaaatata gttcgtacta taccggattt gctattagca
     7321 gcaatctttg tggctgtatt tggaatcggt caaattccag ggatattagc actgtttatt
     7381 ttaactatct gtattattgg aaaattatta tatgaatcat tggaaacgat agatccaggt
     7441 ccaatggaag caatgacggc tgttggcgct aataaaataa aatggattgt tttcggtgtt
     7501 gtaccacaag ccatatcgtc atttatgtca tacgtattat atgcatttga agtaaatata
     7561 cgtgcttcag ctgtgcttgg attagtcggc gctggcggta ttggattgtt ttatgatcaa
     7621 acacttggtt tatttcaata tccaaaaaca gcaacgatta ttttatttac tttagttatt
     7681 gtcgtcgtca ttgattacat cagtacgaaa gtgagggcac atctcgcatg acacaggaaa
     7741 tagcaaaata taatgttcac acaaaagcac acaaacgaaa attgattaaa agatggctta
     7801 ttgcaattgt tgtcttagct attatcatct gggcatttgc aggtgtacca agtttagaac
     7861 ttaaaagtaa atcattagaa atcttaaaat ccatattcag cggattattc catcctgaca
     7921 tcagctatat ctatatacca gatggcgaag acttattacg tggtttactt gaaacctttg
     7981 cgatagccgt tgtaggtact ttcatcgccg caattatctg tattccatta gcatttctag
     8041 gtgcaaataa tatggtaaag ttacgcccgg tttcaggtgt tagcaaattt attttaagtg
     8101 ttatacgtgt cttcccagaa attgtaatgg cacttatatt tatcaaagct gttggcccag
     8161 gttcattttc aggtgtatta gctttaggta tccattctgt aggtatgctt gggaaacttt
     8221 tagctgaaga tattgaaggt ctagatttca gtgctataga atcattaaag gccagtggtg
     8281 cgaataagat taaaacactc gtatttgcag tcataccaca aattatgcct gcctttctat
     8341 ctctcatact ttatcgcttt gaactaaact tacgttcagc ttctatactg gggctaattg
     8401 gggctggtgg tatcgggaca ccactcatat ttgccattca aacacgttct tgggaccgtg
     8461 taggtattat attaatcggt ttagtactaa tggtcgcaat tgtagattta atttccggtt
     8521 caatccgaaa acgtattgtt taacattaaa tcaggatact cctaaataag aggtcctacc
     8581 gtcttacgtt tctctattat aataaaaaca gcagtgaaga aaactattgt tatagttaac
     8641 ttcactgctg tttttattat atctaaattt attctatttc aattccttta aataactttt
     8701 accgaactct ggtaatgtta cgttgaaatt atctgctata gttgcaccga tagaactgaa
     8761 tgtagtatca ctttctagtg catgaccacc tttaaatttc ggactgtaca taattactgg
     8821 gatatattct ctcgtatggt ccgtacctgg cgctgtcggg tcattaccat ggtctgctgt
     8881 aataattact aaatcgtctt cttttaagtt gctaaacagt tctggcaagc gatcatcgaa
     8941 atctttaatt gcttgtgcat aacctggttt atcacgacga tgaccgtata atgcatcaaa
     9001 gtctactaag tttaagaagc taatacctgt gaaatctttc ttaacaattt tcatcaattg
     9061 atccataccg tccatgttac tcttcgtacg aaccgcttct gttacacctt caccatcata
     9121 aatgtcatta attttaccga tggcaataac atcataacca ccgtctttca aatgatctaa
     9181 gacagtttta ccaaaaggtt ttaacgcata gtcatgtcga ttagatgtac gtgtaaagtt
     9241 tcctggttca ccaacatatg gacgtgcgat aatacgacca attaaatatt tagggtcttt
     9301 tgtcaactca cgaacctttt cacaaatatc atataactct tctaatggga taatgtcttc
     9361 atgtgcagca atttgcaata ctgggtctgc acttgtataa acaattaagt cgccagtttt
     9421 catttggtgc tcgccccact catcgataat ttgcgtaccc gatgccggtt tgttagcaac
     9481 aactttacga cctgtcattt cttcaatttg ttgaattaac tcttcaggga atccattagg
     9541 gtatacttta aaaggttgca taatatttaa acccataatt tcccagtgac cagtcattgt
     9601 atctttacca actgaagctt cactcaattt agtatagtat gcttctggtt gttcaactgc
     9661 atttactact ggtaatttat cgatgttccc tagacctaac ttttcaaggt ttggtaaagt
     9721 ttgatcgaaa ccttctaagg tatgtcttaa agtatgtgaa ccttcatctt taaaatcagc
     9781 tgcgtctggc gcttcaccaa tacctactga atccattacg attaaatgta cacgattaaa
     9841 tggtcttgtc atagctatca ctcccaaaat ttatatatat tagtaatctg aatctgcttc
     9901 taaaccttgc ataatttgaa cacctgcgct cgcaccaata cgtgtcgcac ctgcttcaac
     9961 cattttattg aaatcttcta aattacgtac gccacctgat gcttttactt ctacatcagc
    10021 acctactgta tctttcatta atttaacgtc ttctgcagtc gcaccgccac ctgcaaaacc
    10081 tgttgaagtt ttaacgaagt ccgcaccagc cgcttttgtt aattcactcg cttttacaat
    10141 ttcgtcatgg tccaacaata ccgtctcaat aatcactttt actgtgtgac ctttcgcaac
    10201 tttaaccact gcttcaatgt cttgttgtac atcatcaaaa cgtccatctt ttaatgcgcc
    10261 gatgttgatg accatgtcaa tttcatctgc accattttga attgcatctt ctgtttcaaa
    10321 tgctttcgtt gcagttgtcg acgcacctaa tgggaatcct attaccgtac aaacgagcac
    10381 ctctgaatca gctagtcgct ctgctgcata tttaacatgt gttggattca cacatacaga
    10441 tttaaaattg tatgctttcg cttcatcgat gatttgatcg atttgcgtac gtgttgactc
    10501 aggcttcaat aaagtgtgat ctatatattt ctcaaatttc atacttacta ctcctcgtgt
    10561 tatataatct ctttatttaa ttttactata aatacgaata tatctcgcga atttatatct
    10621 atttattttt cagattgtgt ccaacgattt aagctgacga ataaaactaa tccgacaata
    10681 ctcattaaac ctaaaataat taaaataata ccgaaatgtg aacttaatgc atcattgcct
    10741 gggaaattta atgctttaaa atcgattaga gccgcagcaa tcgcaatacc tacagatacc
    10801 gccacattaa taattaaatt ataaaaacca atagccacac ctgtcatatt aagatctatt
    10861 gttttaatgg cttcgttaag taaaggtgca tacattaaag caaagctacc tgcaaagaat
    10921 atcatagaaa tgacgaagat tgaaatgtga ttacctactg caaatgcagg taaaatcaag
    10981 ctcagtgcga ttaaaataat tgctgtgata atcgcttgtt ttgaattcag atattcgccg
    11041 attttaccac ttagtgcacc aacgatgact gctactatat aacccggtac taataacagt
    11101 gatgttgtgt ctagttgcag atgataaatt tgctccatta tgaatgggaa cgtaaaaata
    11161 taacccaatt ggatagcata cattacaaat actataaata aaaatgaagc ataacgttta
    11221 ttttggaaaa atgatttatt tactaatgga cgttgcgcat ttttaatata tagcgcaaaa
    11281 acgataatcg caattaaggc accaatcata tataaccaat taaagttcgt aataaacagc
    11341 atgactgttg tagcaatagt tgccactaga attaagccaa caaaatctaa atgagcttta
    11401 ttcgtattat tttcttttgg taataattta aataggaatg gcaacgtaaa tactacgatt
    11461 aatgcaatta aaaacatatt tgtccagtgt aaatacgtag aaataaatcc acctgataat
    11521 gtaccgataa ctaatgacaa ggaataactg ctcgtactta agccaaggta agtcttctgg
    11581 tcctctttag aaagatactt tgcaacatat atcacatata atgtctctgc agcggctaaa
    11641 ccggcagttt gaataatacg tccaactaaa agtaatgcga attgatgttg gaaaatgtaa
    11701 ccaataattg atccgataat gattaatatc acgccataaa taaatagtgt gcgtatacta
    11761 atggcatcag ataatgaagc gtatactaca gcgccaatac caattactaa accggctaat
    11821 gtcgcttgcc aacttactgt cgttacagaa atgtttaagt catcagcaat ggctacagaa
    11881 atcaatttaa atgagttgtc tataaccaaa ctgaatacaa ataagaataa taaaataggt
    11941 actgctttct ttattttaga atattcaaca ttcataatta tctaaaccaa tgacagtgca
    12001 atttcaatca tatctgtaaa tgcacgttcc ctttcctcag gtgttgttga cgtttcatga
    12061 attaaatgat cgctcactgt gaacacacct aatgcttcga caccagcgta aattgcattc
    12121 atgtataatg cagctgattc catttctaca cctaaaatac ccatacgcat ccaacgttca
    12181 ctcgctgttg tatccgcgtt atagaaaata tcacttgata acacattacc tacatgatga
    12241 cgtacacctt tgtcacgtgc tgttccaact gctttttcta ataattgata agaagcaatt
    12301 ggcgcaaaat gacctggtaa ttgatattgt tgaacgtaat ttgaatcagt agaggcacct
    12361 tgtgcaataa tcacatcata taaatcaatg ttttcttgca tcgcgccaca agaaccaacg
    12421 cgaattaatt ttttacaacc aaatgtatga attaattcat aagagtaaat gccaatagat
    12481 ggcataccca tacctgaacc catgacagaa acttttttac ctttatatgt tccggtaaaa
    12541 ccaaacatgt ttcgcactgt attgaactgt tccacatcat ccaaataagt ttctgcaatg
    12601 aacttagctc ttaacggatc tcctggcaat agaaccgttt ctgcaatttc gacgtcattc
    12661 attggtttaa tgtgtggtgt tgatttcata aaatattcct cccaatattt gtatagtgat
    12721 ttgtctattt gtctaaccat gtatataata atatttttta aaagcgcttt caagcattac
    12781 attttgacaa tattaagaac attttacagt tacagctact aaccttgtat aaaactaa
//
LOCUS       DAMZLE010000069        12553 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMZLE010000069 DAMZLE010000000
VERSION     DAMZLE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.069"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(66..1520)
                     /locus_tag="POB92_001673"
     CDS             complement(66..1520)
                     /locus_tag="POB92_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6579171.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            1724..3241
                     /locus_tag="POB92_001674"
     CDS             1724..3241
                     /locus_tag="POB92_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDI6579172.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            3332..3481
                     /gene="rpmG"
                     /locus_tag="POB92_001675"
     CDS             3332..3481
                     /gene="rpmG"
                     /locus_tag="POB92_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6579173.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            3935..4204
                     /gene="rpsN"
                     /locus_tag="POB92_001676"
     CDS             3935..4204
                     /gene="rpsN"
                     /locus_tag="POB92_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI6579174.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            4361..5338
                     /gene="guaC"
                     /locus_tag="POB92_001677"
     CDS             4361..5338
                     /gene="guaC"
                     /locus_tag="POB92_001677"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI6579175.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            5360..6376
                     /locus_tag="POB92_001678"
     CDS             5360..6376
                     /locus_tag="POB92_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDI6579176.1"
                     /translation="MKKFIVTVVAFLSITIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAARKQHQLFTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(6756..7379)
                     /gene="lexA"
                     /locus_tag="POB92_001679"
     CDS             complement(6756..7379)
                     /gene="lexA"
                     /locus_tag="POB92_001679"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDI6579177.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            7522..7755
                     /locus_tag="POB92_001680"
     CDS             7522..7755
                     /locus_tag="POB92_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579178.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            7891..8130
                     /locus_tag="POB92_001681"
     CDS             7891..8130
                     /locus_tag="POB92_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDI6579179.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            8251..10239
                     /gene="tkt"
                     /locus_tag="POB92_001682"
     CDS             8251..10239
                     /gene="tkt"
                     /locus_tag="POB92_001682"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6579180.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            10517..10759
                     /locus_tag="POB92_001683"
     CDS             10517..10759
                     /locus_tag="POB92_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDI6579181.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            10746..10844
                     /locus_tag="POB92_001684"
     CDS             10746..10844
                     /locus_tag="POB92_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579182.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            10938..11405
                     /locus_tag="POB92_001685"
     CDS             10938..11405
                     /locus_tag="POB92_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDI6579183.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            11529..>12553
                     /locus_tag="POB92_001686"
     CDS             11529..>12553
                     /locus_tag="POB92_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI6579184.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSII"
BASE COUNT         4590 a         1753 c         2262 g         3948 t
ORIGIN      
        1 tttacaccat taaaaagcgg caaatgctat agataatcta aagcatctgc cgtatgagaa
       61 tttatttatt tttgattgtc ataatcgtgt ggttttaaat taattgtttc tagctttaca
      121 aattttgttt tgtgaatgat tttatgaata aagtaaatca acgctagaat gattaaaggt
      181 aaaaagtttt taaaagcatt taaccattga tcttttaaaa tatattcaac tgagccacca
      241 aatagcaaga ataatagtgt agtgatgaca atgattggtc ctaatggata aaaaggtgct
      301 ttatatggta ggaccttatt agggtcttga ccttgttttt taatagcttg tcgcaatcgt
      361 atttgtgacc aaatgcttga tccccaaaca actataatca ttgaaccaat aatttcaagt
      421 aaattaaaaa cggcatttga attaaagttt gcataaataa taacaataac aacgactgca
      481 taagtagtta ataatgctct taaaggtaac ttagttgtct tgtttaattt acttaaaaat
      541 tggggtgctt ttttgtctga acttaaggaa tacaacattc tgcctgttgt ataaacacct
      601 gaatttgcag cggataataa tgaagttaaa ataaccgcgt tgattactga tgctgcaaag
      661 gctatgccta ctctatcgaa tacaattgta aatgggcttt gacttattga actacttgct
      721 cttaataatg atggatctgt gtacggaata attgcaccaa ttactgcaat tgataagaca
      781 tagaataaaa gaatacgcca aaatacttgt ttaattgcct taggcataga ctttttaggg
      841 tcatctgatt caccagcagt tactgctact acttctgtac caccaaccga aaatccggcg
      901 actaataata cgcctaagaa accagagata ccaccaacaa acggtgcttg gccttttgta
      961 tagttttcaa atccatatgt atgaccacct aagataccga aaatcattaa aaagccaaaa
     1021 ataacgaata cgataattgt taacacttta atcaatgata accaaaactc agtttctcca
     1081 aatgatttta cagaaaaaat gtttaataat agtaaaattg taataaagat taagctccaa
     1141 gtaatggggt ggaaaaattt aaatgtgtcc cagaaataaa gcacatttga cgctactatg
     1201 acatcaacac ttgtaactaa tgaccacaat gcccaataca accatcccat ggtaaagcca
     1261 agagatgagt caataaagcg tgttgaataa gagctgaatg aacctgatac tggataaaat
     1321 gttgccaact ctccaattga tgacattaag aaatatagca tgacaccaat aacaagataa
     1381 gcgagtatag cgcctccagg accagcttga gaaatgatat taccagtagc tacaaataga
     1441 ccagtcccaa ttgcaccacc tatagcaatc atggaaatgt gtcttgagtt aagactacgg
     1501 ttcattttat tatcttccat atttagtctc ccatctattt aaatataccc attattgtaa
     1561 gctttttaag tgtactattc aataactatt tagtactgta aagcgaaaaa attaaaattt
     1621 tctgattttt taatcatctt gagcatgttt aattgtaatt ttgatggggt taaattataa
     1681 tatgtattaa attataatta ttataaattg tggagggatg actatgtcac aacaagacaa
     1741 aaagttaact ggtgtttttg ggcatccagt atcagaccga gaaaatagta tgacagcagg
     1801 gcctagggga cctcttttaa tgcaagatat ttacttttta gagcaaatgt ctcaatttga
     1861 tagagaagta ataccagaac gtcgaatgca tgccaaaggt tctggtgcat ttgggacatt
     1921 tactgtaact aaagatataa caaaatatac gaatgctaaa atattctctg aaataggtaa
     1981 gcaaaccgaa atgtttgccc gtttctctac tgtagcagga gaacgtggtg ctgctgatgc
     2041 ggagcgtgac attcgaggat ttgcgttaaa gttctacact gaagaaggga actgggattt
     2101 agtagggaat aacacaccag tattcttctt tagagatcca aagttatttg ttagtttaaa
     2161 tcgtgcggtg aaacgagatc ctagaacaaa tatgagagat gcacaaaata actgggattt
     2221 ctggacgggt cttccagaag cattgcacca agtaacgatc ttaatgtcag atagagggat
     2281 tcctaaagat ttacgtcata tgcatgggtt cggttctcac acatactcta tgtataatga
     2341 ttctggtgaa cgtgtttggg ttaaattcca ttttagaacg caacaaggta ttgaaaactt
     2401 aactgatgaa gaagctgctg aaattatagc tacagatcgt gattcatctc aacgcgattt
     2461 attcgaagcc attgaaaaag gtgattatcc aaaatggaca atgtatattc aagtaatgac
     2521 tgaggaacaa gctaaaaacc ataaagataa tccatttgat ttaacaaaag tatggtatca
     2581 cgatgagtat cctctaattg aagttggaga gtttgaatta aatagaaatc cagataatta
     2641 ctttatggat gttgaacaag ctgcgtttgc accaactaat attattccag gattagattt
     2701 ttctccagac aaaatgctgc aagggcgttt attctcatat ggcgatgcgc aaagatatcg
     2761 attaggagtt aatcattggc agattcctgt aaaccaacct aaaggtgttg gtattgaaaa
     2821 tatttgtcct tttagtagag atggtcaaat gcgcgtagtt gacaataatc aaggtggagg
     2881 aacacattat tatccaaata accatggtaa atttgattct caacctgaat ataaaaagcc
     2941 accattccca actgatggat atggctatga atataatcaa cgtcaagatg atgataatta
     3001 ttttgaacaa ccaggtaaat tgtttagatt acaatcagag gacgctaaag aaagaatttt
     3061 tacaaataca gcaaatgcaa tggaaggcgt aacggatgat gttaaacgac gtcatattcg
     3121 tcattgttac aaagctgacc cagaatatgg taaaggtgtt gcaaaagcat taggtattga
     3181 tataaattct attgatcttg aaactgaaaa tgatgaaaca tacgaaaact ttgaaaaata
     3241 aatttgatat gtagtttcta tattgcgtag ttgagcagtt tatgatatca taataaatcg
     3301 taaagattcc taacaagaga gggtgtttaa cgtgcgcgta aacgtaacat tagcatgcac
     3361 agaatgtggc gatcgtaact atatcactac taaaaataaa cgtaataatc ctgagcgtat
     3421 tgaaatgaaa aaatattgcc caagattaaa caaatatacg ttacatcgtg aaactaagta
     3481 attcttatca ttcaaatacg acgatttgaa aataaagcgg gcttacctat tatattgggg
     3541 agctcgcttt tttatgaaat ttttgtgaag agtgattaat ggattgagtt tcatcggtag
     3601 aacaatatat gattgtatta gttgttactt tattaaaatt tgagaatatt tatagaagga
     3661 aatagattac tgattttata aagtcacttt gttagcgaat gcttgaaaga gtatttaata
     3721 tagtagaatt taaaatttca aagcggaatt taataagtac gaagtaattc tgggtatgtt
     3781 ttataaatgt tcgataatac actttaatct taaatatgat ggtttagaaa atgatttaac
     3841 aaagaaatga aactttactg ttgaattatg tgaggattgt gttattatat aaatcgtaat
     3901 aattacgatt tgataaaaag tgaggtaact atatatggct aagaaatcta aaatagcaaa
     3961 agagagaaaa agagaagagt tagtaaataa atattacgaa ttacgtaaag agttaaaagc
     4021 aaaaggtgat tacgaagcgt taagaaaatt accaagagat tcatcaccta cacgtttaac
     4081 tagaagatgt aaagtaactg gaagacctag aggtgtatta cgtaaatttg aaatgtctcg
     4141 tattgcgttt agagaacatg cgcacaaagg acaaattcca ggtgttaaaa aatcaagttg
     4201 gtaaaatcaa atttcgtact ttagcccatt tacaatatca ataaaacaat gtacagtata
     4261 tacgaatgct ataaactgaa tgttttctca tattaataag gaaacattcg gtttttaatt
     4321 tgcatttaaa aataattagt ttttaaaggg gctatttaaa gtgaaaatat ttgattacga
     4381 agatattcaa ttaataccta ataaatgcat agttgaaagt aggtctgaat gtgatacaac
     4441 tatccaattt ggtccgaaaa aattcaagct acctgtagtt cctgcaaata tgcaaacagt
     4501 tatgaatgag aaattagcga aatggtttgc tgaaaatgat tacttttata tcatgcatcg
     4561 ttttgatgaa gaagcaagaa taccttttat aaaacatatg caaaattcag gcttatttgc
     4621 atctatttca gttggtgtaa agaaagcgga atttgatttt attgaaaagt tagctcaaga
     4681 aaaattaatc cccgaatata ttacaataga tattgcgcat ggtcactcag attcagtgat
     4741 aaacatgatt aaacatataa aaaaccatat acctgatagt tttgttattg ctggtaatgt
     4801 tggcacgcca gaaggtgtta gagaattaga aaatgctggt gctgatgcta ccaaagtcgg
     4861 tataggtcct ggtagagttt gtattacaaa gattaaaaca ggttttggta ctggtggttg
     4921 gcagttagcg gcattaaaca tatgtagtaa agcagctcgt aaacctttga ttgccgatgg
     4981 tggtataaga acgcatggcg acattgctaa atcaattaga tttggtgcat caatggtcat
     5041 gattggttca ttatttgctg cacacgaaga atcacctggt gaaactgtag aacttgatgg
     5101 taaacagtat aaagaatatt ttggtagtgc atctgaattt caaaaaggcg aacataaaaa
     5161 tgtagaaggt aaaaaaatgt ttgtagaaca taagggttca ttaatggata ccttaaaaga
     5221 aatgcaacaa gatttacaaa gctcaatttc atatgccggt ggaaaagact tgaaatcatt
     5281 acgtactgta gattatgtta ttgttagaaa ctctattttc aacggtgata gagattaata
     5341 tttatagtag gtgatgtaaa ttaaaaaatt catagtaact gttgttgcgt ttttatcaat
     5401 tactatcatt gcgccaataa cagaatttaa accattcatt catttacaaa atgaagtaag
     5461 acaatatatt gacattcaca tcaataaaga aacaatttct gcggaaaata aattggatac
     5521 accgaagaaa caacaatttg cctttaataa tatacaaatg aacatgtcga aatcagatgt
     5581 tgagaaaaca ttaaataaac caaaaagagt gacatttaat gaatatggta cgaagtggta
     5641 tacgtattat gatgacgatt acaataattt tataatgata agttacatga aagataaagt
     5701 taatgcgtta tatacaaatc aaaatataat cacttcaaaa tcaaaaatta aatacaatac
     5761 acctaaatcg gttgtaaggc aaagattagg cgaaccagaa acagagattg ttaaaggtag
     5821 agtgcgttac gaacaaaata ataaagaata tgatgttttc cataaaaatc acatttatac
     5881 gacggtattt tatgataagc atcgacgtaa taatgtaaca gctgttttac aagtaagtga
     5941 tgctatggaa aatagattaa aagaacaata tggagcacct tcgaaatcgc ttgcagatag
     6001 ttttgaacta caaaattttg atttagttaa tgctgcaaga aaacaacatc aattatttac
     6061 attgaagtat tctaaacaga attctgaaac tgcacgtaag catagtaaag atatggccaa
     6121 caatcattat tttgatcata caaatttaaa aggtcaatca ccatttgatc gattgaaaaa
     6181 agatggtatt acatttaact cagccggaga gaatttagca tatggtcaag ttagtagtat
     6241 ctatgcacat caaggattaa tgaattctat tggtcacaga aaaaatattt taaatgatac
     6301 gtttaaaata ttaggtgttg gtgttgattt taatgatgaa aaacaacctt tttggacaga
     6361 aaattatact ggttaatgtt tttggcatga gattaaaggg taatgtttgt ctgtaaggaa
     6421 gtagataaac atgatgacaa cagataaacc aaaagatgcg gatatacttg aacgcgtcaa
     6481 agatatactt aataaaaaag aaagaaaaaa ataataatgg ctcgctcctg taaattatta
     6541 cgggggcgag ttttaatttg tgtaacattt tgcagaaatc tgtaattcaa aatttatatt
     6601 aaagaatgag attatctaac aattcgcaat ttctgtttga actgaattta gattagaata
     6661 aataaaacct cttgaaaacg taactttttc aagaggtcca aaatttataa aaatgagggc
     6721 ttaacaatta cgataatata taacggttta aaatattaca tttcgcggta caaaccaatt
     6781 actttcccaa ttacagcaac attgtctagg taaattggct ccattgtact attttcaggt
     6841 tgtaatcgat aacgattttt ttctttatag aagcgtttga cagttgcttc atcttcctca
     6901 gtcatagcaa caataatgtc tccattttct gctatggttt gactgcgaac aattactttg
     6961 tctccgtcta atataccagc ctcaatcata ctgtcgccta cgacgtttaa tatgaatatg
     7021 tcgctattgt gtgtcgatgt taagtgttca ggtaatggaa aatattcttc aatattttct
     7081 actgcggtaa taggaacacc tgctgtgact ttaccaataa ctggcacatg aatcgtttct
     7141 tccatattaa tattatcatt tgtttgatca cttacaattt ctatagcacg tggtttcgtt
     7201 ggatctcttc ttatatagcc tttttcttca agacgtgaaa ggtgaccatg aacagttgaa
     7261 ctggatgcta agccaactgc ttcaccaatt tcgcgaacac taggcggata acctttcgtt
     7321 tgaacaactt gtttaatata gttatatatt tcgctttgtc gttttgttaa ttctctcata
     7381 tataggcact ccctaaatta atttaaatac agtatatcat taatttgaaa ccaaaacaaa
     7441 catttgttcg ttaaaatatt gacacagaac ataagttctg ataaattact aatacaaaca
     7501 aatgttctag gagtgaaaat gatgttttac aataaatata aaaacgtatc aacatatatc
     7561 atcatatttt tagtttcaag tgcagccttt gcaatattct tgttaagtgc gaacattagt
     7621 gctcactcgg aacaagtgta cgaaatgact gaccatcaaa ttaagaacaa tacgataaat
     7681 aaagcatacg aacataaaga ccctacaaac aatagcgaac aaagagatgg gaaagtgttc
     7741 gctttaataa attgatacat tgtcacaacg ttattttgcc tatttttgcg aaatagcgtt
     7801 ttttattact tttttgctga tcttaaattt gttatatttt gttaaagtat tataatgatt
     7861 gaataaacaa attgaaggta ggttttttaa ttgagtaatt ctgatttgaa tatcgaaaga
     7921 attaacgagt tagctaaaaa gaaaaaagaa gtaggattaa ctcaagaaga agcaaaggag
     7981 caaacagcct taagaaaagc ttatcttgag agttttagaa aagggtttaa acaacaaatt
     8041 gaaaatacta aagtaattga tccagaaggt aatgatgtaa cacctgaaaa aattaaagag
     8101 atacaacaaa aaagagataa taaaaattaa atcacaaatc tgtaaagaat tttctgacat
     8161 tataacttga aataagtatt ttacttatct ttttatttta aaataagtta taatgtattt
     8221 gataaaattg aagaagggaa gatacacaag atgtttaatg aaaaagatca attagctgtt
     8281 gatacgctac gtgcactaag tatcgacaca atcgaaaaag cgaattctgg tcatccagga
     8341 ttacctatgg gagctgcccc aatggcttac actttgtgga cacgtcatct gaattttaat
     8401 ccacaatcta aagattactt caatagagac cgtttcgtat tatctgcagg gcatggttca
     8461 gcattattgt atagcttgtt acatgtttct ggtagtttag aattagaaga attaaagcaa
     8521 tttagacaat ggggttctaa aacaccaggt catcctgaat acagacatac agatggtgta
     8581 gaagttacta ccggaccact tggacaaggt tttgctatgt cagtaggatt agctttagca
     8641 gaagatcacc tagcagggaa atttaataaa gaaggatata atgttgtaga tcattacaca
     8701 tatgtattag cttctgacgg tgatttaatg gaaggtatat cgcatgaagc agcttcattt
     8761 gctggacata ataaattaag taaattagtt gttttatacg attcaaatga tatttcatta
     8821 gatggcgaat taaacaaagc tttttctgaa aacacaaaag ctcgttttga agcatatggt
     8881 tggaattact tactagttaa agatggtaat gatttagaag aaattgataa agcgattact
     8941 acagctaaat ctcaagaagg accaacgatt attgaagtta aaacaacaat cggatttggt
     9001 tcaccgaata aagcaggaac taatggtgtt catggggcac ctttaggtga agttgaaaga
     9061 aaattaacat tcgaaaatta cggtttagat cctgaaaaac gttttaatgt ttcagaagag
     9121 gtatacgaaa ttttccaaaa tactatgtta aaacgtgcta atgaagatga atctcaatgg
     9181 aattcattat tagaaaaata tgcagaaaca tatcctgaat tagcagaaga atttaaatta
     9241 gcgattagtg gtaaattgcc taaaaattat aaggatgaat taccacgttt tgaactgggt
     9301 cataatggtg catctcgtgc tgattctggt actgttattc aagcaatcag taaaactgtc
     9361 ccttcattct ttggtggatc agcagacctt gctggttcaa acaaatccaa tgtaaatgat
     9421 gcaacagatt atagttctga aacacctgaa ggtaaaaatg tgtggtttgg tgtacgtgaa
     9481 tttgctatgg gtgctgctgt aaatggtatg gctgcacatg gaggtttaca tccatatggt
     9541 gcaacattct tcgtatttag tgattattta aaaccagcgt tacgtttatc atcaattatg
     9601 ggattaaatg caacgttcat cttcacacat gattcaattg cagtaggtga agatggtcct
     9661 actcatgaac caattgaaca attagctgga ttaagagcta ttccaaatat gaatgttatc
     9721 cgtcctgctg atggtaatga aacaagagta gcatgggaag ttgccttaga atctgaatct
     9781 acacctactt cattagtatt gacacgtcaa aacttaccgg ttttagatgt accagaagat
     9841 gtagttgaag aaggcgttcg aaaaggtgcc tatacagttt atggctctga agagacacca
     9901 gaattcctat tattagcttc aggttcagaa gttagtcttg cagttgaagc tgctaaagat
     9961 cttgaaaaac aaggtaaatc agtgcgtgtt gtttcaatgc ctaactggaa tgcatttgaa
    10021 caacaatctg aagaatataa agaatcagtt attccatcaa gcgtaacaaa acgtgttgcg
    10081 attgaaatgg cttcaccgct tggatggcat aaatatgtag gtactgcagg taaagttatt
    10141 gctattgacg gctttggcgc aagtgcacct ggcgatttag tagttgaaaa atatggattt
    10201 actaaagaaa atatcttaaa ccaagttatg agcttataag aataatttat aaagcgagta
    10261 tgtttagaag tctaggatgc ataatcttag gcttctttta aagtgttgaa atttagagta
    10321 tagcacttaa actacatcat aagtgataag ttatgaaagt atactatttc agattaatct
    10381 ttaaaagctc tgttataaca gcatgatttt tgatattatt tttagtatcg atattaaaat
    10441 acttgaataa actagttctt gaaataatgt gatgaattta gtaaaattca gtaggataaa
    10501 gaaagcgggt gaaacaatgg caacttggtt agcaattatt tttatagtag ctgcattaat
    10561 tttaggttta attggaggtt tccttttagc tagaaaatat atgatggact acttgaagaa
    10621 aaacccacca atcaacgaag aaatgcttcg tatgatgatg atgcaaatgg gtcaaaaacc
    10681 ttctcagaag aaaattaatc aaatgatgac gatgatgaat aaaaatatgg atcaaaatat
    10741 gaagagtgcg aaaaagtaaa ttcgcaattg atagaggcta ttttccagat atggaaatgg
    10801 cctcttttta taatcaaatt aataagaata aatatgttta ttaaaattaa agttaacaaa
    10861 atgacgaata gactgagaaa tgctataatt cattttgtat gatttacaga gagtttattt
    10921 aacgagaagg tgtctgcgtg ctctatttaa tattttcaat cattgtagct ttatttatgg
    10981 gaactatagt tatagttatt cgtatgaaag ctcaaaatta tccggtaaat gagaaaaaaa
    11041 tagttttgcc accgtttttt atggcgaccg gtgcattgat gtacgtcgtt ccatatttta
    11101 ggctaacagg atcggaaatg ctagaagcct ttataattgg tttgcttttt tctacagttc
    11161 taatttggac ttctcgattt gaagtcaaag gtacagaaat ttatatgaaa cgatctaaag
    11221 catttccagt tattttgatt tcattactta tcattcgtac tgtgatgaaa atattcatta
    11281 gtaatgaaat agatcctgga gaattaggcg gcatgttctt tttattagca ttctgtatga
    11341 ttgttccttg gagagcagca atgctatata aatacaaaaa actaaagaaa acattaatca
    11401 attaattact tttaaaacca cttgtgatcg acttctaaat cagtcaatga gtggttttaa
    11461 ttttacttga aaaggtgaaa tgattatatt aaaataaaag cgaacaaatg ttctatatgg
    11521 aggcagtaat gaaaattata catacagcag actggcactt agggaaaata ttaaatggca
    11581 aacagctttt agaagatcaa gcgtatattt tagatatgtt cgtagaaaaa atgaaagaag
    11641 aagaacctga tatcattgtg atagctggag atttatatga cacaacatat ccaagtaaag
    11701 atgcaatcat gttattagaa caagcgattg gaaagttaaa tttagaactg cgtataccaa
    11761 taattattat tagtggaaat cacgatggta aagagaggtt aaactatggg gcgagttggt
    11821 ttgaacataa tcagttattt ataagaacag attttacatc gattaattca ccaatagaga
    11881 taaatggggt taatttttat acactccctt atgctactgt gagcgaaatg aaacactact
    11941 ttgaagatga caccattgaa acacatcaac agggaattac gcgctgtatt gaaacaatag
    12001 caccggaaat tgatgaagat gccgtcaata ttttaattag tcatctgact gttcaaggtg
    12061 gaaagacatc tgattctgaa agaccattaa ctattggaac ggttgaatca gttcagaaag
    12121 gtgtttttga tatatttgat tatgtcatgc taggtcactt gcatcatcca tttagtatag
    12181 aagacgacaa aattaaatat agtggctcct tattgcagta ttcattttcg gaagcgggtc
    12241 aagctaaagg gtatagacgt gtaacaatta atgatggcat tattaacgat gtatttattc
    12301 ctcttaagcc acttagacaa ttggaaatta tctcaggcga atataatgat gttattaatg
    12361 aaaaagttca tgtgaaaaat aaagataatt atttacattt taaacttaaa aatatgtctc
    12421 atattactga tccaatgatg agtttaaaac aaatttatcc taatacttta gcgctgacga
    12481 atgaaacttt taattacaat gaagaaaata atgctataga aataagtgaa aaagatgaca
    12541 tgtcaattat cga
//
LOCUS       DAMZLE010000070        12434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMZLE010000070 DAMZLE010000000
VERSION     DAMZLE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.070"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            129..4439
                     /locus_tag="POB92_001687"
     CDS             129..4439
                     /locus_tag="POB92_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6579185.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            4729..5196
                     /gene="rimP"
                     /locus_tag="POB92_001688"
     CDS             4729..5196
                     /gene="rimP"
                     /locus_tag="POB92_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI6579186.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            5217..6392
                     /gene="nusA"
                     /locus_tag="POB92_001689"
     CDS             5217..6392
                     /gene="nusA"
                     /locus_tag="POB92_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI6579187.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTIVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            6413..6697
                     /locus_tag="POB92_001690"
     CDS             6413..6697
                     /locus_tag="POB92_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDI6579188.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            6694..7011
                     /locus_tag="POB92_001691"
     CDS             6694..7011
                     /locus_tag="POB92_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDI6579189.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            7016..9133
                     /gene="infB"
                     /locus_tag="POB92_001692"
     CDS             7016..9133
                     /gene="infB"
                     /locus_tag="POB92_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI6579190.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKMAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            9519..9869
                     /gene="rbfA"
                     /locus_tag="POB92_001693"
     CDS             9519..9869
                     /gene="rbfA"
                     /locus_tag="POB92_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI6579191.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            10039..10956
                     /gene="truB"
                     /locus_tag="POB92_001694"
     CDS             10039..10956
                     /gene="truB"
                     /locus_tag="POB92_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI6579192.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLEKKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            10971..11942
                     /gene="ribF"
                     /locus_tag="POB92_001695"
     CDS             10971..11942
                     /gene="ribF"
                     /locus_tag="POB92_001695"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDI6579193.1"
                     /translation="MKVIEVTHPIQSKQYNTEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            12057..12326
                     /gene="rpsO"
                     /locus_tag="POB92_001696"
     CDS             12057..12326
                     /gene="rpsO"
                     /locus_tag="POB92_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI6579194.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT         4594 a         1702 c         2453 g         3685 t
ORIGIN      
        1 gttttaacaa tattatgatt agccaaatat aaagcattta attttctatc attactatgc
       61 ctacataatc taaatattgt tcgaacacgt aaaagtaatt tctatttaag gtggtaattg
      121 tcttggcaat gacagagcaa caaaaattta aagtgcttgc tgatcaaatt aaaatttcaa
      181 atcaattaga tgctgaaatt ttaaattcag gtgaactgac acgtatagat gtttctaaca
      241 aaaacagaac atgggaattt catattacat taccacaatt cttagctcat gaagattatt
      301 tattatttat aaatgcaata gagcaagagt ttaaagatat cgccaacgtt acatgtcgtt
      361 ttacggtaac aaatggcacg aatcaagatg aacatgcaat taaatacttt ggacactgta
      421 ttgaccaaac agctttatct ccaaaagtta aaggtcaatt gaaacagaaa aagcttatta
      481 tgtctggaaa agtattaaaa gtaatggtat caaatgacat tgaacgtaat cattttgata
      541 aggcatgcaa tggaagtctt atcaaagcgt ttagaaattg tggttttgat atcgataaaa
      601 tcatcttcga aacaaatgat aatgatcaag aacaaaactt agcttcttta gaagcacata
      661 ttcaagaaga agacgaacaa agtgcacgat tggcaacaga gaaacttgaa aaaatgaaag
      721 ctgaaaaagc gaaacaacaa gataacaacg aaagtgctgt tgataagtgt caaattggta
      781 agccgattca aattgaaaat attaaaccaa ttgaatctat tattgaggaa gagtttaaag
      841 ttgcaataga gggtgtcatt tttgatataa acttaaaaga acttaaaagt ggtcgccata
      901 tcgtagaaat taaagtgact gactatacgg actctttagt tttaaaaatg tttactcgta
      961 aaaacaaaga tgatttagaa cattttaaag cgctaagtgt tggtaaatgg gttagggctc
     1021 aaggtcgtat tgaagaagat acatttatta gagatttagt tatgatgatg tctgatattg
     1081 aagagattaa aaaagcgaca aaaaaagata aggctgaaga aaagcgtgta gaattccact
     1141 tgcatactgc aatgagccaa atggatggta tacccaatat tggtgcgtat gttaaacagg
     1201 cagcagactg gggacatcca gccattgcgg ttacagacca taatgttgtg caagcatttc
     1261 cagatgctca cgcagcagcg gaaaaacatg gcattaaaat gatatacggt atggaaggta
     1321 tgttagttga tgatggtgtt ccgattgcat acaaaccaca agatgtcgta ttaaaagatg
     1381 ctacttatgt tgtgttcgac gttgagacaa ctggtttatc aaatcagtat gataaaatca
     1441 tcgagcttgc agctgtgaaa gttcataacg gtgaaatcat cgataagttt gaaaggttta
     1501 gtaatccgca tgaacgatta tcggaaacga ttatcaattt gacgcatatt actgatgata
     1561 tgttagtaga tgcccctgag attgaagaag tacttacaga gtttaaagaa tgggttggcg
     1621 atgcgatatt cgtagcgcat aatgcttcgt ttgatatggg ctttatcgat acgggatatg
     1681 aacgtcttgg gtttggacca tcaacgaatg gtgttatcga tactttagaa ttatctcgta
     1741 cgattaatac tgaatatggt aaacatggtt tgaatttctt agctaaaaaa tatggcgtag
     1801 aattaacgca acatcaccgt gccatttatg atacagaagc aacagcttac attttcataa
     1861 aaatggttca acaaatgaaa gaattaggcg tattaaatca taacgaaatc aacaaaaaac
     1921 tcagtaatga agatgcatat aaacgtgcaa gacctagtca tgtcacatta attgtacaaa
     1981 accaacaagg tcttaaaaat ctatttaaaa ttgtaagtgc atcattggtg aagtatttct
     2041 accgtacacc tcgaattcca cgttcattgt tagatgaata tcgtgaggga ttattggtag
     2101 gtacagcgtg tgatgaaggt gaattattta cggcagttat gcagaaggac cagagccaag
     2161 ttgaaaaaat tgccaaatat tatgatttta ttgaaattca accaccggca ctttatcaag
     2221 atttaattga tagagagctt attagagata ctgaaacatt acatgaaatt tatcaacgtt
     2281 taatacatgc aggtgacaca gcgggtatac ctgttattgc gacaggaaat gcacactatt
     2341 tgtttgaaca tgatggtatc gcacgtaaaa ttttaatagc atcacaaccc ggcaatccac
     2401 ttaatcgctc aactttaccg gaagcacatt ttagaactac agatgaaatg ttaaacgagt
     2461 ttcatttttt aggtgaagaa aaagcgcatg aaattgttgt gaaaaataca aacgaattag
     2521 cagatcgaat tgaacgtgtt gttcctatta aagatgaatt atacacaccg cgtatggaag
     2581 gtgctaacga agaaattaga gaactaagtt atgcaaatgc gcgtaaactg tatggtgaag
     2641 acctgcctca aatcgtaatt gatcgattag aaaaagaatt aaaaagtatt atcggtaatg
     2701 gatttgcggt aatttactta atttcgcaac gtttagttaa aaaatcatta gatgatggat
     2761 acttagttgg ttcccgtggt tcagtaggtt ctagttttgt agcgacaatg actgagatta
     2821 ctgaagtaaa cccgttaccg ccacactata tttgtccgaa ctgtaaaacg agtgaatttt
     2881 tcaatgatgg ttcagtagga tcaggatttg atttacctga taagacgtgt gaaacttgtg
     2941 gagcgccact tattaaagaa ggacaagata ttccgtttga aacattttta ggatttaagg
     3001 gagataaagt tcctgatatc gacttaaact ttagtggtga atatcaaccg aatgcccata
     3061 actacacaaa agtattattt ggtgaggata aagtattccg tgcaggtaca attggtactg
     3121 ttgctgaaaa gactgctttt ggttatgtta aaggttattt gaatgatcaa ggcatccaca
     3181 aaagaggtgc tgaaatagat cgactcgtta aaggatgtac aggtgttaaa cgtacaactg
     3241 gacagcatcc agggggtatt attgtagtac ctgattacat ggatatttat gattttacgc
     3301 cgatacaata tcctgccgat gatcaaaatt cagcatggat gacgacacat tttgatttcc
     3361 attctattca tgataatgta ttaaaacttg atatacttgg acacgatgat ccaacaatga
     3421 ttcgtatgct tcaagattta tcgggcattg atccaaaaac gatacctgta gatgacaaag
     3481 aagtcatgca aatatttagt acacctgaaa gtttaggtgt tactgaagat gaaattttat
     3541 gtaaaacagg tacgtttggg gttccagaat tcggtacagg gttcgtgcgt caaatgttag
     3601 aagatacaaa gccaacaaca ttttctgaat tagttcaaat ctcaggatta tctcatggta
     3661 cagatgtgtg gttaggcaat gctcaagaat taattaaaac cggtatatgt gatttatcaa
     3721 gtgtaattgg ttgtcgtgat gatatcatgg tttatttaat gtatgctggt ttagaaccat
     3781 caatggcttt taaaataatg gagtcagtac gtaaaggtaa aggtttaact gaagaaatga
     3841 ttgaaacgat gaaagaaaat gaagtgccgg attggtattt agattcatgt cttaaaatta
     3901 agtacatgtt ccctaaagcc catgcagcag catacgtttt aatggcagta cgtatcgcat
     3961 atttcaaagt acatcatcca ctttattact atgcatctta ctttacaatt cgtgcgtcag
     4021 actttgattt aatcacgatg attaaagata aaacaagcat tcgaaatact gtaaaagaca
     4081 tgtattctcg ctatatggat ctaggtaaaa aagaaaaaga cgtattaaca gtcttggaaa
     4141 ttatgaatga aatggcgcat cgaggttatc gcatgcaacc gattagttta gaaaagagtc
     4201 aggcgttcga atttatcatt gaaggcgata cacttattcc gccgttcata tcagtgcctg
     4261 ggcttggcga aaacgttgcg aaacgaattg ttgaagctcg tgacgatggc ccatttttat
     4321 caaaagaaga tttaaacaaa aaagctggat tatctcagaa aattattgag tatttagatg
     4381 agttaggctc attaccgaat ttaccagata aagctcaact ttcgatattt gatatgtaaa
     4441 atgaaataat caaggtattt atttaatgcg tatggcgtag ttaaagaaat acaaaattgt
     4501 tgctggacat aaaattatgc ctgtatttct tttcaacgtc ttacgagtct attcgaatgt
     4561 aatggtgaaa taaatgaaca aacttttaca agaatctctg attaatagtg aagtcatttg
     4621 tttcaagcat aaacttatgc tataattaag ttgcttaaaa attagtgaac tcaggcagaa
     4681 gagtgggaga ttcccgctct tttctatttg ccaaaaaggg aggcctgtat gagtaaaatt
     4741 acagaacaag tagaagtgat tgttaaacca attatggaag acttgaattt tgaacttgta
     4801 gacgttgaat atgtcaaaga gggtagagat cattttctta gaatctctat tgataaagaa
     4861 ggtggcgtag atttaaatga ttgtacgcta gcttctgaaa aaataagtga agctatggat
     4921 gcaaatgatc ctattcctga aatgtattat ttagacgtag cgtcacctgg tgcagaacgt
     4981 ccaattaaaa aagaacaaga tttccaaaat gcaataacta aacctgtatt tgtttcttta
     5041 tatgtaccaa ttgaaggtga aaaggaatgg ttaggcattt tacaagaagt caataatgaa
     5101 acaattgtag tacaagttaa aatcaaagca agaacgaaag atatagagat accgagagac
     5161 aaaatagcaa aagcacgtca cgcagttatg atttaacgtg atgaggagga aaaaacgtgt
     5221 caagtaatga attattatta gctactgagt atttagaaaa agaaaagaag attcctagag
     5281 cagtattaat tgatgctatt gaagcagctt taattactgc atacaaaaag aattatgata
     5341 gtgcaagaaa tgtccgtgtg gaattaaata tggatcaagg tactttcaaa gttatcgctc
     5401 gtaaagatgt tgttgaagaa gtatttgacg acagagatga agtggattta agtacagcgc
     5461 ttgttaaaaa ccctgcatat gaaattggtg atatatacga agaagatgta acacctaaag
     5521 attttggtcg tgtaggtgct caagcagcga aacaagcagt aatgcaacgt cttcgtgatg
     5581 ctgaacgtga aattttattt gaagaattta tagacaaaga agaagacata cttactggaa
     5641 ttattgaccg tgttgaccat cgttatgtat atgtgaactt aggtcgtatc gaagctgttt
     5701 tatctgaagc agaaagaagt cctaacgaaa aatatattcc taacgaacgt atcaaagtat
     5761 atgttaacaa agtggaacaa acgacaaaag gtcctcaaat ctatgtttct cgtagccatc
     5821 caggtttatt aaaacgttta tttgaacaag aagttccaga aatttacgat ggtactgtaa
     5881 ttgttaaatc agtagcacgt gaagctggcg atcgctctaa aattagcgtc ttctctgaaa
     5941 acaatgatat agatgctgtt ggtgcatgtg ttggtgccaa aggcgcacgt gttgaagctg
     6001 ttgttgaaga gctaggtggc gaaaaaatcg acatcgttca atggaatgaa gatccaaaag
     6061 tatttgtaaa aaatgcttta agcccttctc aagttttaga agttattgtt gatgaaacaa
     6121 atcaatctac aatagttgtt gttcctgatt atcaattgtc attagcgatt ggtaaaagag
     6181 gacaaaacgc acgtctagct gctaaattaa ccggctggaa aattgatatt aaatcagaaa
     6241 cagatgcgcg cgaagcgggt atctatccag tagttgaagc tgaaaaagta actgaagaag
     6301 atgttgcttt agaagatgct gacacaacag aatcaaccga agaggtaaat gatgtttcag
     6361 ttgaaacaaa tgtagagaaa gaatctgaat aataggttgg agtgaagtat ctatgaaaaa
     6421 gaaaaaaatt ccgatgcgaa aatgtattct ttcaaatgaa atgcatccca aaaaagatat
     6481 gattcgtgtt gttgttaata aagaaggcga aatctttgcg gatgttactg gaaagaaaca
     6541 aggccgtggc gcatatgttt ctaaagatgt tgctatggtt gaaaaagcac aacaaaaaga
     6601 aattttagaa aaatatttta aagcatctaa agagcaattg gatcctgttt ataaagaaat
     6661 tattagatta atttatagag aagagatccc aaaatgagta tagatcaaat attaaacttt
     6721 ttaggattag caatgagagc tggtaaagta aaaacaggtg aatcagtcat tgttaatgag
     6781 ataaaaaaag gaaatttaaa gctcgttatt gttgcaaatg atgcgtctga taatacagct
     6841 aaattaatta cagataaatg taagagttac aaagttccat tcagaaagtt tggaaatcga
     6901 aatgaattgg gaatagcact tggaaaaggt gagcgtgtta atgtagggat tactgaccca
     6961 ggctttgcta aaaagttgct atcaatgata gatgaatatc ataaggagtg attatatgag
     7021 taaacaaaga atttacgaat atgcgaaaga attaaatcta aagagtaaag agattataga
     7081 tgagttaaaa agcatgaata ttgaggtttc aaatcatatg caagctttgg aagatgacca
     7141 aattaaagca ttagataaaa agttcaaaaa agaacaaaag aacgacaata aacaaagcac
     7201 tcaaaataat caccaaaaat caaacaatca aaaccaaaat aaagggcaac aaaaagataa
     7261 caaaaagaat caacaacaaa ataataaagg caacaaaggc aataaaaaga ataatagaaa
     7321 taataagaaa aataacaaga ataataaacc acaaaatcaa ccagctgctc caaaagaaat
     7381 accatcaaaa gtgacatatc aagaaggtat tacagtaggc gaatttgcgg ataaattaaa
     7441 tgttgaatca tcagaaatta tcaaaaaatt attcttactt ggtattgttg ctaatatcaa
     7501 tcaatcatta aatcaagaaa caatcgaatt aattgccgat gattatggcg ttgaggttga
     7561 agaggaagtt gtgattaatg aagaagattt atcaatctac ttcgaagacg aaaaagatga
     7621 tccagaagca attgagagac cagcagttgt aacaattatg ggacatgttg accatggtaa
     7681 aactacttta ttagattcaa ttcgtcatac aaaagttaca gcaggtgaag caggtggaat
     7741 cactcaacat attggtgcat atcaaattga aaacgatggc aaaaaaatca ctttcttaga
     7801 tacaccggga catgctgcat ttacaacgat gcgtgcgcgt ggtgcacaag taacagatat
     7861 tacaatttta gtagtagcag ctgacgatgg tgttatgcca caaacaattg aagcaattaa
     7921 ccatgctaaa gaagcagaag taccaattat tgttgcagta aataaaattg ataaaccaac
     7981 ttcaaatcct gatcgagtta tgcaagaatt aactgaatat ggtttaattc ctgaagattg
     8041 gggtggcgaa acaattttcg ttccactttc tgcattaagt ggtgatggta tcgacgattt
     8101 attagaaatg ataggattag ttgcagaagt tcaagaactt aaagcaaatc ctaaaaaccg
     8161 tgctgttggt acagttatcg aagctgaatt agataaatca cgtggtcctt ctgcatcatt
     8221 attagtacaa aacggtacat taaatgttgg ggatgcgatt gtagttggta atacttacgg
     8281 ccgtattcgt gcaatggtta atgacttagg tcaaagaatc aaaatggctg gtccatcaac
     8341 gcctgttgaa attacaggta ttaatgatgt gccacaagct ggggatcgct ttgttgtatt
     8401 tagtgatgaa aaacaagctc gtcgtattgg tgaatcaaga cacgaagcta gcattgttca
     8461 acaacgtcaa gaaagtaaaa atgtttcatt agataacctg tttgaacaaa tgaaacaagg
     8521 tgaaatgaaa gatttaaacg ttattattaa aggtgatgtt caaggttctg ttgaagcttt
     8581 agctgcatca ttaatgaaaa ttgatgttga aggcgtaaat gttcgtatca ttcatacagc
     8641 ggttggtgca attaatgagt cagacgtgac acttgctaat gcctcaaatg gtattatcat
     8701 tggtttcaat gttcgtccag acagtggtgc aaaacgtgct gcagaagctg aaaatgttga
     8761 tatgcgttta cacagagtta tttataatgt tatcgaagaa attgaatcag cgatgaaagg
     8821 tttacttgat ccagaatttg aagaacaagt tatcggacaa gctgaagttc gtcaaacatt
     8881 caaagtttct aaagttggta ctattgctgg atgttatgtt actgaaggta aaattacgcg
     8941 aaatgctggt gtacgtatta ttcgtgatgg tattgttcaa tatgaaggcg aattagatac
     9001 acttaaacgt ttcaaagatg atgctaagga agttgcaaaa ggttatgaat gtggtattac
     9061 aattgaaaac tacaatgacc ttaaagaagg cgatgttatc gaagcatttg aaatggttga
     9121 aattaagcgt taattaaata aattacaagc taaaagtata gttaagattg atatgctccc
     9181 tataaatatt gcacttttta agtgtctact ttatagggag catatttgat actagctttt
     9241 ggttttttat tagaatagat tacctattaa aagttacgtt atatggacat gattttgtat
     9301 aaaattttgt ggtggcctag aatgattttt aatgacaaaa tataatgtcg actattattg
     9361 gaaaattttc tgttgaaatg cctatcttac ggcaaacttt atttgatttt ataggcttaa
     9421 tttattaaaa taacgtgtga gctaaaataa ttgtttaagc attgttacac taaaaaatgc
     9481 aaataacaat tgaacttaaa gataaagagg tgacaagaat gagcagtatg agagcagagc
     9541 gtgttggtga acaaatgaag aaggaattaa tggatatcat caacaataaa gtcaaagatc
     9601 ctcgagttgg ttttattaca attacagatg ttgttttaac aaatgattta tcgcaggcta
     9661 aagtattttt aactgtatta ggtaacgata aagaagtaga aaatacattt aaagcacttg
     9721 ataaagcaaa aggcttcatt aagtctgaat taggttctag aatgcgatta cgtattatgc
     9781 cggaattaat gtatgaatat gatcaatcaa tcgaatatgg caataaaatt gaacgaatga
     9841 ttcaagattt acacaaacaa gatagataat ttagtgttag gtatctggaa aatgtttgat
     9901 aatttcttaa tatcggtata ttaacattaa acagttaata catagatgtg tagaaatagt
     9961 taacattttc cagttttttt atgaataaat tcagttgata cgctattaaa atatatttta
    10021 aaaaaagaag gtgactatat gtataatggg atattaccag tatataaaga gcgcggttta
    10081 acaagtcatg acgttgtatt caaattgcgt aaaatattaa aaactaaaaa aataggtcac
    10141 acgggtacgc ttgatcccga agttgcaggc gtgttaccgg tatgtatagg taatgcaacg
    10201 agagttagtg attatgttat ggatatgggc aaagcttatg aagcaactgt atcgatagga
    10261 agaagtacaa cgactgaaga tcaaacgggt gatacattgg aaaaaaaagg tgtacactca
    10321 gcagatttta ataaggacga tattgaccga ttgttagaaa gttttaaagg tatcattgaa
    10381 caaattccgc cgatgtactc atccgtcaaa gtaaatggta aaaaattata tgaatatgcg
    10441 cgtaataatg aaacagttga aagaccaaag cgtaaagtta atattaaaga cattgggcgt
    10501 atatctgaat tagattttaa agaaaatgag tgtcatttta aaatacgcgt catctgtggt
    10561 aaaggtacat atattagaac gctagcaact gatattggtg tgaaattagg ctttccggca
    10621 catatgtcga aattaacacg aatcgagtct ggtggatttg tgttgaaaga tagccttaca
    10681 ttagaacaaa taaaagaact tcatgagcag gattcattgc aaaataaatt gtttccttta
    10741 gaatatggat taaagggttt gccaagcatt aaaattaaag attcgcacat aaaaaaacgt
    10801 attttaaatg ggcagaaatt taataaaaat gaatttgata acaaaattaa agaccaaatt
    10861 gtatttattg atgatgattc agaaaaagta ttagcaattt atatggtaca ccctacaaaa
    10921 gaatcagaaa ttaaacctaa aaaagtcttt aattaaagga gatagaattt atgaaagtca
    10981 tagaagtgac acatcctata caatcaaaac agtataatac agaggatgtt gcaatggcat
    11041 tcggattttt cgatggcatg cataaaggtc atgacaaagt ctttgatata ttaaacgaaa
    11101 tagctgaggc acgcagttta aaaaaagcgg tgatgacatt tgatccgcat ccgtctgtcg
    11161 tgttgaatcc taaaagaaaa cgaacaacgt atttaacgcc actttcagat aaaatcgaaa
    11221 aaattagcca acatgatatt gattattgta tagtggttaa tttttcatct aggtttgcta
    11281 atgtgagcgt agaagatttt gttgaaaatt atataattaa aaataatgta aaagaagtca
    11341 ttgctggttt tgattttact tttggtaaat ttggaaaagg taatatgact gtacttcaag
    11401 aatatgatgc gtttaatacg acaattgtga gtaaacaaga aattgaaaat gaaaaaattt
    11461 ctacaacttc tattcgtcaa gatttaatca atggtgagtt gcaaaaagcg aatgatgctt
    11521 taggctatat atattctatt aaaggcactg tagtgcaagg tgaaaaaagg ggaagaacta
    11581 ttggcttccc aacagctaac attcaaccta gtgatgatta tttgttacct cgtaaaggtg
    11641 tttatgctgt tagtattgaa atcggcactg aaaataaatt atatcgaggg gtagctaaca
    11701 taggtgtaaa gccaacattt catgatccta acaaagcaga agttgtcatc gaagtgaata
    11761 tctttgactt tgaggataat atttatggtg aacgagtgac cgtgaattgg catcatttct
    11821 tacgtcctga gattaaattt gatggtatcg acccattagt taaacaaatg aacgatgata
    11881 aatcgcgtgc taaatattta ttagcagttg attttggtga tgaagtagct tataatatct
    11941 agagttgcgt atagttatat aaacaatcta taccacacct ttttcttagt aggtcgaatc
    12001 tccaacgcct aactcggatt aaggagtatt caaacatttt aaggaggaaa ttgattatgg
    12061 caatttcaca agaacgtaaa aacgaaatca ttaaagaata ccgtgtacac gaaactgata
    12121 ctggttcacc agaagtacaa atcgctgtac ttactgcaga aatcaacgca gtaaacgaac
    12181 acttacgtac acacaaaaaa gaccaccatt cacgtcgtgg attattaaaa atggtaggtc
    12241 gtcgtagaca tttattaaac tacttacgta gtaaagatat tcaacgttac cgtgaattaa
    12301 ttaaatcact tggtatccgt cgttaatctt aatataacgt ctttgaggtt ggggcatatt
    12361 tatgttccaa ccttaattta tattaaaaaa gctttttaca aatattaaca tttattatat
    12421 gttaagctaa tatt
//
LOCUS       DAMZLE010000071        12283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMZLE010000071 DAMZLE010000000
VERSION     DAMZLE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.071"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..376
                     /locus_tag="POB92_001697"
     CDS             <1..376
                     /locus_tag="POB92_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HDI6579195.1"
                     /translation="SQNIFTEDVANKLDEHNIYYGRDLPIIGSSGVVHNFDFFISAKK
                     NQKEKFINAISNPNNSMIIKSKITDAMQAKKIKRHRQNEFIFILNDSKKEINEHNKNL
                     LHENYISTIDYSELDEKIGLLI"
     gene            complement(1621..1709)
                     /locus_tag="POB92_001698"
     tRNA            complement(1621..1709)
                     /locus_tag="POB92_001698"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1672..1674),aa:Ser,seq:gga)
     gene            complement(1764..1835)
                     /locus_tag="POB92_001699"
     tRNA            complement(1764..1835)
                     /locus_tag="POB92_001699"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:Glu,seq:ttc)
     gene            complement(1837..1911)
                     /locus_tag="POB92_001700"
     tRNA            complement(1837..1911)
                     /locus_tag="POB92_001700"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1877..1879),aa:Asn,seq:gtt)
     gene            complement(1926..1999)
                     /locus_tag="POB92_001701"
     tRNA            complement(1926..1999)
                     /locus_tag="POB92_001701"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1965..1967),aa:Gly,seq:tcc)
     gene            complement(2015..2090)
                     /locus_tag="POB92_001702"
     tRNA            complement(2015..2090)
                     /locus_tag="POB92_001702"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2055..2057),aa:His,seq:gtg)
     gene            complement(2104..2176)
                     /locus_tag="POB92_001703"
     tRNA            complement(2104..2176)
                     /locus_tag="POB92_001703"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2141..2143),aa:Phe,seq:gaa)
     gene            complement(2193..2268)
                     /locus_tag="POB92_001704"
     tRNA            complement(2193..2268)
                     /locus_tag="POB92_001704"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2233..2235),aa:Asp,seq:gtc)
     gene            complement(2290..2363)
                     /locus_tag="POB92_001705"
     tRNA            complement(2290..2363)
                     /locus_tag="POB92_001705"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2327..2329),aa:Met,seq:cat)
     gene            complement(2833..3387)
                     /locus_tag="POB92_001706"
     CDS             complement(2833..3387)
                     /locus_tag="POB92_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDI6579196.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(3709..5109)
                     /locus_tag="POB92_001707"
     CDS             complement(3709..5109)
                     /locus_tag="POB92_001707"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDI6579197.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLIKKPIQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(5133..6056)
                     /gene="hemH"
                     /locus_tag="POB92_001708"
     CDS             complement(5133..6056)
                     /gene="hemH"
                     /locus_tag="POB92_001708"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDI6579198.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(6114..7151)
                     /gene="hemE"
                     /locus_tag="POB92_001709"
     CDS             complement(6114..7151)
                     /gene="hemE"
                     /locus_tag="POB92_001709"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDI6579199.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(7141..7251)
                     /locus_tag="POB92_001710"
     CDS             complement(7141..7251)
                     /locus_tag="POB92_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579200.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            7414..7917
                     /gene="traP"
                     /locus_tag="POB92_001711"
     CDS             7414..7917
                     /gene="traP"
                     /locus_tag="POB92_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDI6579201.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPTKYDVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(8041..9264)
                     /locus_tag="POB92_001712"
     CDS             complement(8041..9264)
                     /locus_tag="POB92_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579202.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(9257..9997)
                     /locus_tag="POB92_001713"
     CDS             complement(9257..9997)
                     /locus_tag="POB92_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579203.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHKQTLDDIYIHVTQGGDVHA"
     gene            10131..10553
                     /locus_tag="POB92_001714"
     CDS             10131..10553
                     /locus_tag="POB92_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDI6579204.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            10695..11060
                     /locus_tag="POB92_001715"
     CDS             10695..11060
                     /locus_tag="POB92_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6579205.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            11793..>12283
                     /locus_tag="POB92_001716"
     CDS             11793..>12283
                     /locus_tag="POB92_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDI6579206.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTILFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYG"
BASE COUNT         4116 a         2206 c         1775 g         4186 t
ORIGIN      
        1 gtcacagaac atatttacag aagatgttgc aaacaaattg gatgaacata acatttatta
       61 cggaagagac ttacctatta taggaagcag tggtgttgtt cataatttcg acttttttat
      121 tagcgctaag aaaaatcaaa aagaaaaatt tatcaatgct atttctaacc ctaataattc
      181 tatgattatt aagtcgaaaa taacggatgc tatgcaagca aaaaaaataa aaagacacag
      241 gcaaaatgag tttattttta ttttaaatga ctcaaaaaaa gaaataaatg aacataataa
      301 aaatcttctt catgaaaact atattagtac aatagattat agcgaattag atgaaaagat
      361 aggtttattg atttaatatt tatagacgtg ataatatcaa tgtttatatt aaatgaaacg
      421 aatttgaaaa attcgaaaca gcttaagata gcaaattgaa tggccttatt gataatgcag
      481 aatcgtctgc acttagttga acaaattcta tgagaataga tattgttaaa ctatgtggat
      541 aggtgatttt attttgacaa gagtcagaag aacatatatt aaggatttca aattacaaat
      601 ggttaaactt tatgaaaatg gtataacaag aatcgaaatt atacgcgaat acgatttaac
      661 actctcaatc ttctcgaact gaataaatac tgggtcattc aatcatcaag acaacttatt
      721 aagtgatgat aaatgttcat accattttgc aaatataacc agatactctc aacaaaatgt
      781 agagttcaaa attaaaaatg tccctaattg gcacattgct gtatgatatt aaattcaact
      841 tagatttcaa caatataact aatgaagctt atatttaccg cgatatcaaa ccaaacagcc
      901 tatccacaaa caaattagaa tataccatgt tagtaatagt aaatgatata tatcttccaa
      961 agaccgcaaa ctgtagcaga aaatgagaac cttgcgtttt tgaaatggtc taataaatca
     1021 aatatcattg aaactttaat cacataaatt aacagtagtc ccctgttcgc agttctataa
     1081 attacaaatg caataaaggc taaagtaaac taattacttt agcctttatt aataatttag
     1141 tcgaatttat taaaaatcga ctaactcctg gttatgctac gcttttcttt aaaatccgca
     1201 cgtagatgcc gattgattat attataatat tattatttta ttttttcaag cgtatatata
     1261 atgtttgatt ttctttaagt aacagctaaa cattacattt caaaacgatt aggtcatgca
     1321 gatgtacata caacgcttaa aacatattcg catttgatta aagaatttga agataatgaa
     1381 actaaattta tagaaaaaaa tctgaatgat ttgttttgtg actaaacttc aaaatcttgt
     1441 gactaaagtt aagattaatc atcaaaaatt agtcacattg tggctaaaaa attttaaatg
     1501 gatataaata aataagaagt gtaaaaatga ggataatagc tcaaatgcag atatatcaag
     1561 ggtttgagtc taaaataaaa atagcgctta aaatgggtaa aaccaattta agcgccttta
     1621 cggaaggtaa gggattcgaa cccttgagac gcttgtgacg cctacacact ttccaggcgt
     1681 gctccttcgg ccagctcgga caaccttcct caacaataaa tatgtaataa aaaaacagaa
     1741 gcgatatttc acttctgttt gtatgactcc tacgggactc gaacccgtgt taccgccgtg
     1801 aaagggcggt gtcttaaccg cttgaccaag gagccatggc tccacaggta ggactcgaac
     1861 ctacgaccga tcggttaaca gccgatagct ctaccactga gctactgtgg aataatagaa
     1921 aataatggag cgggtgatgg gaatcgaacc cacaacatca gcttggaagg ctgaggtttt
     1981 gccattaaac tacacccgct aatgtaaacg attatggggc ggctgaaggg gatcgaaccc
     2041 tcgaatgtcg gaaccacaat ccgatgtgtt aaccacttca ccacaaccgc catgaattaa
     2101 gaatggttca ggacagagtc gaactgccga cacatggagc ttcaatccat tgctctacca
     2161 actgagctac tgaaccttaa tggtaataat aatggcggtc tcgacgggaa tcgaacccgc
     2221 gatctcctgc gtgacaggca ggcgtgttaa ccgctacact acgagaccta ttaaattaaa
     2281 aactatgtat tgcgggaggc ggatttgaac caccgacctt cgggttatga gcccgacgag
     2341 ctaccgaact gctccatccc gcgctaatat tattttgaat tacctaatta atatatcata
     2401 atcaaaaacc taaagtcaag aactttttga atttaattta aatgttatct cttaaataat
     2461 tacttaaata tcgtagcaac atgttctctg ttgaacacaa atattagtat attcattttt
     2521 gtagtatacg tcaacgacat ttttaaagtt ttttgtgtaa aaaaacgctt cttattccct
     2581 tttatcatat aagtgtctaa tagttgtcat aaatagtgtt aaagcattta aaaaggtata
     2641 ggagttataa agtttacaac gcctatacct tctgaaaaag aaattataaa agcttgttac
     2701 accgcatatt ctttcagtca gcgactacca atataacatt gtagccctaa gacattgctt
     2761 gacgcctcaa ttaccacaat tttttcaaaa tcagcagcta cctactgaca caacatagca
     2821 accaaacatt acctatcgtg tcatgtaatc ttgcatccga tcttgcaacg ctgtaaatgt
     2881 ttcgaagcca tcctcttcta agaagtgccc tccatcttcc acgattcgca agttcccctc
     2941 taatgcattc attaaacgct gggtttcttt atatgaaacg tatttgtcat ttttagaact
     3001 caatccgtaa aaattgtcaa ctttcttttt aatattatcg taatcaatgg ttacattact
     3061 taaatcaata tctaaatcta tattttctgc atcttcttta aagcccgcta tactaaaaaa
     3121 gccttcaatc ggctgatcaa tcatttcaat atattttaaa gctgtgattg aacctaaacc
     3181 atgtgttaca aaatatgtat cctttttgcg tacattaatt tgtttcgtca tagcttcaat
     3241 ccactgatcc actgtcttcg cttcagggga ttcaaaatta aataatgtta cgtcatatcc
     3301 ttctaaagtt aagttatgct ccaaccactg ataccaatga tttctactat ttccatgcat
     3361 agaatgtaca ataattacat ctgtcatctc attctctcct ttcaacttac tacttctttt
     3421 ctatttttaa aaaaatgact gattacctat aattgtaaaa taaaaacacc ttaattagaa
     3481 atgttatatc gcaaagtgac atttctaatt aaagtgtatt gtcatcattt caatatcatt
     3541 caaaaacagc taaacctttg tctctgcttc aaattcacaa aaataattcc cgttgaaagt
     3601 atctatattt acacattact tccaccatta tataacttaa aaatgactat atttcatcaa
     3661 acattatcta aaggcgtcgc acctacacca caccatccaa caattaactt acaactctgc
     3721 gattacttct tcagcagcaa ctttaccttg cgtaatacaa tcaggtagtc caaccgcttc
     3781 aaaagatgca ccagttactc taagtcgtgg atatgtttgt ttaatatgtg cttgaatctg
     3841 tctaatttgt tgaatgtgac caacatggta ctgtggcata cttttcggca aacgattgac
     3901 aattgtaaat tcaggatcgc ctttaaatgt catcatttga cttaaatctc tacgtacaat
     3961 cgatactaat tcattatctg tatgatcatc aaccacagta tcacctggtt tacctacata
     4021 cgcacgaatc aaaaccttac cttctggtgt agtaaatggc cattttttcg atgtccaagt
     4081 acatgcggta atgtctgtat cactcgttct cgcaatcacg aagccagtac catcataagt
     4141 attttcaata tctttttcat caaatgccaa tacaacagtt gcaacagtcg tactatccat
     4201 cgttttaaag taatcaaatg ctggatcttg tccgaaccaa ttcaaaaaga cttgatgcgg
     4261 tgttgtcact aataccccat cgaatacatc ttcttgttga ttactgtaaa caattttata
     4321 ttgcttttga gatgtaataa tatcatccac tgacgtattg tagcgtattg tcacaccttt
     4381 atttttcaca tcttgttcta atgcttcaat aaatgaactt aaaccatgct taaattgttt
     4441 gaattgtcct tttggtgcgc caggatataa ttgtctttgt ttcagacgct tatttttctc
     4501 atccttcata ccttttatca gacttccgaa tgcctcttct ttttctttaa aattaggaaa
     4561 cgtactcatc aaacttaatt tatcaatatc ggtaccataa ataccaccca ttaaaggctc
     4621 tattaaattc tcaagtacct cattacctaa tcttgctctg aaaaatgcac caacagaaat
     4681 gtcaccatct tgcatttgta taggcttttt gattaaatct aatcctgctc ttaatttacc
     4741 aagtggtgaa attaatttag ttgtcacaaa cggtttgata tctgtcggaa ttcccataat
     4801 tgatccacct ggaataggat acaatttgtt tttcgcaaaa atatatgatt gtccagtcgt
     4861 atttgtaaca atatcttgtt ctaatccaat atctttcgct aattctgtca taatcgtttt
     4921 tctacctaaa taagattcag gccctagttc aatcatataa ccatctttac gatacgattg
     4981 aatctttccc cccggacgat tcgatgcttc aaagatggtt acatcaatat taggatcttg
     5041 ctgttttaaa aaatatgcac ttgataaacc tgttatcccc gctcctataa tagccactga
     5101 tttagtcaca acgtttcacg cttctttcgt cattaaaata tagacttgat ttcatcaaca
     5161 attgcaccga taaataatgg atgtgtattc ggcatttttg gacgataata attcgcacca
     5221 atatcatcgc aaactacttt acattcataa tcattgtcat aaagcacctc taaatgctca
     5281 catacaaaac ctactggcgt atatataaag tttttatact gatgtttttc atataaatca
     5341 cgtgttaaat cttgtacatc tggccctaac caaggtgtac ctgtattacc ttcagattgc
     5401 caaccaatcg cgatatgttc aatattagat tgttctttaa ttaaaagcgc agtatgttct
     5461 agttcttgtg gatatggatc attattcttt tcgattaaac cttttggcaa actatgtgcc
     5521 gaaacaacta ataccgtgtc tttatgttcc tcttccggta tttgagctaa tgtttcgttg
     5581 actttattcg tccaatattc aataaattta ggttgttcat aataatgttt cacatgtgta
     5641 agttgaatac catattttgc agcttcttca tcagcacgtt tgtcatatga tcctactgaa
     5701 aatgaagaat aatgtggtgc tagtactacc gtgattgctt cagtaatgcc atcattgtgc
     5761 atttgttcaa ccgcatcttc gataaatggt gaaatgtgtt ttaatcctaa gtatagttta
     5821 aattcaacat ctgcatatgc tttatttaat gctgaaacta gcgcatcagc ctggtcatct
     5881 gttgtacctg ctaatggtga taaaccacct ataaattcat atctatcttt caaatcttga
     5941 agttcttctt cagatggacg tttaccatgt ctaatatctg tataatatgg ctctatgtca
     6001 ctttctttat aaggtgtgcc ataagccata actaataatc ccattttttt agtcattgat
     6061 aataccttcc tttaaatgaa ttatctttca tgtgcttcaa tgtaaaacta tgattatctt
     6121 tgtgtatatg tgtgtacgaa ttcgcttact ttacgtaacg tctctggttg cacttctggg
     6181 aatacaccgt gtcctaaatt aaagatgtgt ttaccgttct ccataccttg atctaatatt
     6241 ggtttcaatc tctcttcaat gacattccat ggtgctaata aaattgatgg atctaaattc
     6301 ccttgtaatg ttttagtaac gcctaattgt tgagcctgat taatagacgt tctccaatct
     6361 aggcctaata catcaatcgg taaatcattc cattcattga ttaaatgact ggcacctaca
     6421 ccgaataaaa ttaccggcac atcatgtttt tctttaacct cactgattaa tcgaatcata
     6481 tgtggtttaa tgtaacgtct gtaatcctcg acatttaatg cacctaccca tgaatcgaaa
     6541 atttgaatca attcggcacc tgcttcgact tgagctgtta catatttaac agatacatca
     6601 actaaatgat tcattaaagc aaaccatgtt gcttcatctc tatacatcat cgcttttgta
     6661 aaattgtaat ttttcgatgg tccgccttca atcatatatg acgctaatgt aaatggtgcc
     6721 ccagtaaatc ctattagcgg cacatttaac ttttcttctg ttaaaagttt aattgtatct
     6781 aatacatatg gtacatctcg ttcggggtct atttgagaaa gtttctcaac atcttgaatt
     6841 gttttgattg gattatgaat cactggacca atacccgatt taatttctac atcgacacca
     6901 attggcttta atggtgtcat aatatctttg tataaaattg ctgcatctgt atgataatta
     6961 tcaactggta aatgtgttac ataagcgcac aactccggct gatgtgtaat atcgaatagt
     7021 gaatattttt ctttcaattt tcgatattct ggttgcgaac ggccagcttg tcgcataaac
     7081 caaacaggtg tatgtgatgt ttcttcacct ttgatcattt ttaaaattgt attgttttta
     7141 ttatgcacca taaaggcctc ctaaattaaa atcattctta tctatattat catatcgctc
     7201 attcgttcgt attttcaata aataaatgtc ataaaactga catttaatca tagaactatt
     7261 tattgtaaat ttaaattcta aagtccatta ttttgtatca ttacttctaa atatctcgca
     7321 agattcatta tagtaatttt aatcaattat taatagtggt aatgactagt ttatcatcgt
     7381 ataataaata aaaacataag ggggaccttt catatgaaga aactatatac atcttatggc
     7441 acttatggat ttttacatca gataaaaatc aataacccga cccatcaact attccaattt
     7501 tcagcatcag atacttcagt tatttttgaa gaaactgatg gtgagactgt tttaaaatca
     7561 cctacaaaat atgatgttat caaagaaatt ggtgaattca gtgaacatca tttctattgt
     7621 gcaatcttca ttccatcaac agaagatcat gcatatcaac ttgaaaagaa actgattagt
     7681 gtagacgata atttcagaaa ctttggtggc tttaaaagct atcgtttgtt aagacctgct
     7741 aaaggtacaa catacaaaat ttattttgga tttgctgatc gacatgcata cgaagacttt
     7801 aagcaatctg atgcctttaa tgaccatttt tcaaaagacg cattaagtca ttactttggt
     7861 tcaagcggac aacattcaag ttattttgaa agatatctat acccaataaa agaatagatt
     7921 cgaagaaaac ttccattggt tatgctgtta acgtatagat gctaatgtaa tctgaacgcc
     7981 gtatcatttg gcgttcaaca ttacatgcac ctttacaaca tacatgccaa tggaagtttt
     8041 ttagtctcgt aataatgttt cctgatattt caacttttta ataatacttc taatcgttaa
     8101 taaacctaca atatagaaaa tgagtacaac atagaataac gtcatatgtt ttataataaa
     8161 tgtcactgca aacacagtac aaataacaaa cattaatcta tataaaaact gttcataccc
     8221 tttgattacc ttctcttcag gtacaggcca cacttgaggc cataagccat atgcttgctg
     8281 tgaataaaat tgtgccattt gtaacaatat aatatataca aataaacacc caataattgc
     8341 tgtcactaat ggatatgata accaaaccat taataaaact gcaataatta ctaacctaaa
     8401 gataatatta aatgcgtctc tccctcttat aaagcttcta ataaataaga ataaatacat
     8461 cgcattagag ttaaatttac taccctttgg aactggtaaa agtatatcta gataacttct
     8521 tctgactgca gattctttca aatgttttac atcggtgaac atattaacaa atttataata
     8581 attcatatga tgtcgatgtt cgattgcaat cattttctcc caaggataca aaaagcctgg
     8641 tttatatttt ttaactaaaa attctattaa cacaggcaaa gcaaccatca caaatgcgat
     8701 gtaccatttt ggagctaata gtaagtaata tgttagagca aaggtgatga atgatattaa
     8761 attaacttgc catgttttaa gtcccgattg ataccattgc catcttaagc gtaaaccaac
     8821 atatggaaaa attaatgcac tgactccaaa acaaatataa aatgccacat tatgttgatt
     8881 aatattgtaa aacaacggga acattacaat aacaataatg agttggatta atatgcgcgc
     8941 aaagtaacta tataaaatcg catgacgcat aaattgagac atgtgttttt caaatggtaa
     9001 taaaaagatt ttatccgctt cttttaggag tggtcgcatt ggaaaaatgg atgtcaatgc
     9061 tacaatcact gctgctatta atgaaaaatt gatattcgtt ggaatatgtt ttaaccattc
     9121 accatatcca aaaataaatg cacccagcaa aataagtaaa aagaccatga aatgaccatt
     9181 aaatataaac ttattataat aatttttctc tttacgaagg gcatgtaatc ttttattaaa
     9241 taatgtggtc gcttggttat gcatgtacat ctccaccttg cgtcacatga atatatatat
     9301 cgtctaatgt ttgtttatgt aagccagttt gttgtctcaa tgcttctaaa tctccaaatg
     9361 caacgacttc accttcgtct agtatgataa aacgatcaca ataacgttca gctgttgcta
     9421 aaatatgtgt actcattaga acggttctac cttcgttttt cttttcaacc attaaatcta
     9481 acatggattg aattcctaat ggatctaggc caaggaatgg ttcgtctata atatacaatt
     9541 cgggattaac gataaacgca caaataatca tgactttttg tttcatcccc ttagaaaaat
     9601 gactcggaaa aactttcaat tcattttcta aacggaatgt ctttaataat ggcattgctc
     9661 gattcatcgc ttcatcacga tcaatatcat atgccattgc tgtcatctca atgtgttcct
     9721 ctaatgtgag ttcttcataa ataaccggtg attccggaat ataagataac tttcttctat
     9781 aagcctctat gtcatcatta atgttgatat ctgaaattga tagagatcct tccataggtg
     9841 taagcaatcc tagcatatgt ttaatcgttg tactcttacc agcgccatta aggccaataa
     9901 gtccaacgat ttcgcctttg tttaattcaa aatttatatc tttaattaca gggcgttttc
     9961 catatccacc tgtaagctgt tctactttaa ctgtcataag gcacctccat gacttatatt
    10021 gtaccaaaaa ttataaaatc ctcatattaa atacacatgt cctaatatcg aatttttagc
    10081 gacaatgtta taatgaatgg taatacaagt tgaaaaggag tgtagtcatc atgtcagaaa
    10141 caattttcgg caaaatttta actggagaaa ttcctagctt taaagtatat gaagacgatt
    10201 atgtctatgc ctttttagat atatcacaag ttactaaagg acatacgtta ttaattccta
    10261 aaaaagcttc tgctaatatc tttgaaactg atgaagaaac aatgaaacat atcggtgcag
    10321 cattacctaa agtagcaaat gctattaagc gtgcatttaa tcctgatggt ttaaacatta
    10381 ttcaaaataa tggtgagttt gcagatcaat ctgtatttca tattcatttc cacttaattc
    10441 ctcgatatga aaatgatatt gatggatttg gttataagtg ggaaacacat gaagacattt
    10501 tagataacga tgcaaaacaa caaattgctg aacaaattca agcacaattt taaatgtatg
    10561 cttaatctaa gctcgaacgg gtataatatg attaatatta taacaattgc gtttgaagtg
    10621 ataacatcaa ggttagcaat tttaaacaaa atgagttatc aagataacag atgttaaaag
    10681 tgaggagaat ataaatgaaa gcatcacgca ttctattcgg tatcggtgtt ggcgtagcag
    10741 ctggttttgt agttgcactt caaggacgag acgacaaaag tgtcaagaac aacacgatcg
    10801 atcgtactgc ccctactggt tcaaaatcag aactacaacg tgaatttgaa acgattaaac
    10861 aaagttttaa tgacatttta aactatggtg ttcaaattaa aaacgaaagt gcggaatttg
    10921 gtagttcaat tggtggtgaa attaagtcat tacttggaaa cttcaaatct gacattaatc
    10981 ctaatattga acgtttacag tcacacatcg aaaatttaca aaatcgtggc gaggatattg
    11041 gaaacgaaat ttctaagtag cacgttacgt tctcgatcac aactattatt attagtaaca
    11101 gcatatttat tttttttaat taaatgccaa ataaacgaga tgacattaga aattagatat
    11161 ttcttgtcat ctctttttta taactcaaat gaacttatgt ttacaaatta caggaataca
    11221 tcgtttgtag tgattttcgc ttaaatcata tttatgaatt gattgaaaac attgcttagg
    11281 attcattgtg ttatccttgc actttgatta cgctttactt aaatcattat cgacaaacaa
    11341 catacttata ttttcattga gccgaacctt ttatgcacat tacatatacc ttacttgcac
    11401 aaattattaa tctggtattt attataatta catatcacta tatttttagc atttgtataa
    11461 cttagttggt caaaagatgc ttttgcatat gccctgccat atccattttt tatattattc
    11521 aagttttcaa atactcatgt cacacgcgcc tttttcataa gtgatgcttt attagcaaga
    11581 agatgtgttc gcagaaattt gttctgcatt ctacttctac gctagtcaat cagccaattt
    11641 taccaaactc cactttcgcg tttcaaatca aacaatactt cgcccctttc ttcttatata
    11701 acaattcttc taacatgata tgttactatt gaattactga acctgagtta gttataatct
    11761 aacttatatt gaaaagagat gaggcgtaag atatgttttt atgtaaaaga caaattgata
    11821 tcaatgcacg atttggtttg cctagaattg catttatgag tgcagttgca accatcatta
    11881 tgtttttagt tagttatgaa gtaatgtatt ttttatctaa tacgccatta tcagatagac
    11941 attttctcat ctttttatta cttgtattta tgacgtatcc attacataaa agtatacatt
    12001 tgttattttt cttaccatat agaaaatcgt ttaaagttca taagttgact aaaagaaaat
    12061 ggcttatatt ctataatacc tacgtcaatc aacctgtaca caaattttat ttttgcatta
    12121 acttaatatt gccgttaatt atcttatctg caatgttcgt ttatctaaca attttattcc
    12181 cgcaatatgg acattatttt atgttcttat tggcattgaa tttcggtatt tccattacag
    12241 atttattata tttaaaaata attatatttt ctaattatgg aca
//
LOCUS       DAMZLE010000072        12255 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMZLE010000072 DAMZLE010000000
VERSION     DAMZLE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.072"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            49..744
                     /locus_tag="POB92_001717"
     CDS             49..744
                     /locus_tag="POB92_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6579207.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            746..1516
                     /locus_tag="POB92_001718"
     CDS             746..1516
                     /locus_tag="POB92_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579208.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNKIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1890..3854
                     /locus_tag="POB92_001719"
     CDS             1890..3854
                     /locus_tag="POB92_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDI6579209.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNADEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            4196..5275
                     /locus_tag="POB92_001720"
     CDS             4196..5275
                     /locus_tag="POB92_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579210.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5380..5973)
                     /locus_tag="POB92_001721"
     CDS             complement(5380..5973)
                     /locus_tag="POB92_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6579211.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(6004..6963)
                     /locus_tag="POB92_001722"
     CDS             complement(6004..6963)
                     /locus_tag="POB92_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDI6579212.1"
                     /translation="MANNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            complement(7040..7474)
                     /locus_tag="POB92_001723"
     CDS             complement(7040..7474)
                     /locus_tag="POB92_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6579213.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7700..7984
                     /locus_tag="POB92_001724"
     CDS             7700..7984
                     /locus_tag="POB92_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6579214.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8158..8963
                     /locus_tag="POB92_001725"
                     /pseudo
     CDS             8158..8963
                     /locus_tag="POB92_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(9084..9755)
                     /locus_tag="POB92_001726"
     CDS             complement(9084..9755)
                     /locus_tag="POB92_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6579215.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10025..11017
                     /locus_tag="POB92_001727"
     CDS             10025..11017
                     /locus_tag="POB92_001727"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6579216.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLEDDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTSTCETRLN"
     gene            complement(11264..12064)
                     /locus_tag="POB92_001728"
     CDS             complement(11264..12064)
                     /locus_tag="POB92_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6579217.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKSRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFKIASAVAPSNDKQGVLTIIEQEVLNK"
BASE COUNT         3990 a         2185 c         1912 g         4168 t
ORIGIN      
        1 tataaaatct taagattcag attatttaat agcaaaggag atagtgatat ggatgtttta
       61 acaatagaac atttaacaaa gaagataggc aacaaaacga ttctcgaaga tgtatcattt
      121 aagctgaaac gcggacaaat agttggtctc gttggagcga atggtgcagg taaaacaact
      181 ttaatgaaag ttatattagg ttactctagt ttccaaagcg ggaattttaa tgttattaac
      241 agcaaagaca gcaaaagcaa tatcggtgca ttgattgaaa atccaggaat atatcctttt
      301 atgtctggat atgaaaactt gaagttattg aatgaatcaa aaaacactca agatatcgat
      361 aaaattgtct cacaacttca tatggatgaa tacattcata aaaaagctaa aacgtattct
      421 cttggtatga aacaaaaatt aggaattgct atagcatttt taaatgaacc tcaatttatt
      481 atcttagatg aaccaatgaa tggcttagat ccaaaagctg tgcgagatgt tcgtgaattg
      541 attgtccaaa aagcgcaaga aggtgttact ttcttaattt cgagtcatat tttaagtgaa
      601 ttagttaaaa tcacaaactc tatccttatt attaacaaag gtaaaattgt tacagaaaca
      661 tcggaagaag aacttaaaca atttaaagat aatgatttag aaaatgtatt actagaaatc
      721 atagaaaggg aggaccaagc ataaaatggg aactttaatt aaacaagaat gtttcaaatt
      781 atttaaaaag aaatcaactt ttatcgcacc tattgtcttt attctactaa tggttgctca
      841 aggttatatt gctacaaaat acaataaaat ttttacgcca caggaatctt tcacatctgc
      901 ttataatggt ttttcatggt ttgcattttt attaattatt caagcaagta caatcatttc
      961 aatggaattt cattacggta cgattaaaaa tttactctat cgtgaatatt caagaacaac
     1021 tatgattgtt agcaaaatca tcacattatt tattatttct ttaatttatt ttgttattac
     1081 aattattgct tcaattgtta ttgggtcttt attctttaat gatttaaata tatttgaaag
     1141 tagcggtaat caattatctt tattgaatca attattatta gttagtttag gcacatttgt
     1201 tggcgtttgg ttagttttaa gcttaacgtt gctattatca tctgcaacaa attcaacggg
     1261 agtagccatt gctgtaggta ttgtttttta ttttgcaagt tctattttag cagttattca
     1321 aacggcactt ttagaaaaaa tagactggct aaagtggaat cctattaata tgatgaatat
     1381 tatgcttcaa acagttgaaa aaggctttag taagtcgacg aaattagaac ttcatgaatt
     1441 gtttattggt aatattgctt atatttctat tttcttaata cttgtagtat ttattttcaa
     1501 gaagaaaaat atttagtaac ttcaagttat taaatgtcta aatacacaca tattccatcg
     1561 taattcaaaa tcattttcaa atcccttcac ccaaataatg gtgcggggat ttttttatcc
     1621 aaattttggc aattccacat tttacatatc gtaatttttt aggaaactag tgaatataac
     1681 aaattcctcc tctcattttt aaaataaata tatcactttc ccacttttac ttaactaaac
     1741 tgcaacgact cctaatacca aaatcctgcc ctctattttt atcaatccaa gcatacttat
     1801 tgaaaaatgt taacgttttc ttgataatca ttgtaagcgc atttatttta taaactaacg
     1861 tttgaaatat actacaggag tgacacgtaa tgactcaaat tactgaaaaa gaattaaaaa
     1921 agaagtattt agatttacta tcccaaaatt ttgatactcc agaaaaactt gcaactgaaa
     1981 ttatcaattt agaatcaatt ttagaattac ctaaaggtac ggaacatttc gtcagcgatt
     2041 tacatggtga atacgaagct ttccaacacg tattacgcaa cggttctggg aatgtgcgag
     2101 cgaaaatcaa tgatattttc aaagagagac tttcaactaa ggagcttaat gatttaactg
     2161 ctcttgtcta ctatccagaa gacaaattaa aattgattaa aagtgatttc caaagttgcg
     2221 gtcaacttaa tgtctggtat atcacaacaa tcgaacattt aattgagttg attaaatatt
     2281 gttcttcaaa atatacgcgt tcaaaactgc gtaaagcatt gccaaaacaa tacgtttata
     2341 ttattgagga actactgtat aaaagtaatg aatatcaaaa taaaaaatca tattacgaaa
     2401 cacttgttaa ccaagtaatt gaacttaaac aggcagatga tttaattatt ggactcgctt
     2461 attccgtaca acgcttagtc gtcgatcatt tacacgttgt cggtgatatt tatgatcgtg
     2521 gaccacaacc agataaaatt atggatacac tgattaatta tcattcccta gatattcaat
     2581 ggggtaatca tgatgtgctt tgggttggag cctatgctgg gtcaaaagta tgcttagcaa
     2641 acttacttcg aatttgtgca cgctatgata atttagatat tatcgaagac gcttatggca
     2701 ttaatttaag accactgctt actttagctg aaaaatacta tgacgcagat aatcctgctt
     2761 ttaagcctaa aaaaagacct gacaaacacg aacgtttaac tcaacgtgaa gaaagtcaaa
     2821 ttactaaaat tcatcaagct attgcgatga ttcaattcaa gttagaaata ccaattatta
     2881 aacgtcgtcc aaatttcgaa atggaagaac gtcttgtgct tgaaaaggtt aattatgata
     2941 caaatgaaat tacagtttat ggtaatacat acccattgaa agacacatgt ttccaaactg
     3001 tcaatcgtga taatccagca gaattactac ctgaagaaga agaagtcatg aataaactat
     3061 tattgtcatt ccaacaatct gaaaaattac gtcgtcatat gtctttcttg atgcgtaaag
     3121 gctctcttta cttaccatat aatggcaatt tactcattca tggttgtatt ccagttgatg
     3181 aaaatggtga gatggaatca tttgaaattg atggtcatac ttacagcggc caagaattat
     3241 tagatgtatt tgagtatcat gtccgtaaat catttgatga aaaagaaaat actgatgact
     3301 tatcgacgga tttagtttgg tatttatgga ctgggaaata ttcgtcatta tttggaaaac
     3361 gtgccatgac tacgtttgaa cgatacttta ttgcagataa agcttctcat aaagaagaaa
     3421 aaaatccata ctatcatcta cgtgaagatg tgaatatggt tcgtaaaatg ctcagtgatt
     3481 ttggattaaa tgcagatgaa ggtcgcatta ttaatggtca cacaccagtg aaagaaatca
     3541 atggcgaaga tcctatcaag gctgatggaa agatgcttgt cattgatggt ggcttttcaa
     3601 aagcttatca gtcaacaact ggcattgcag gctatacact attgtataat tcattcggta
     3661 tgcaactcgt tgcacaccaa caatttaacg ctaaagaaaa aatactttcc gaaggtatcg
     3721 atgaactttc tataaagcgc gtcgtagata aagaattaca acgtaagaag attcgtgata
     3781 ccaatattgg taaagatctt caagctcaaa tagatatttt gaaaatgtta atgcatgatc
     3841 gctacttaga ctaaagacga cattttatgc atgtattgta tactgttgta ttgatgcggt
     3901 ttacgcattg gctacgcaaa cgcgatgata aataggcgac ctagctacac cgaaaaaaat
     3961 cccctcacta ttgcgagtag tgaggggatt ggtgtatcta tgctaattcc tgttaactgt
     4021 attataacaa ctttacagca aaaaaatgca aaatcaacta ataaattaat gaaatgaccg
     4081 acgccctcgc ttctacttca atttaacaaa atactatatt agtactatat ttttcattaa
     4141 catgtatgtt acattttcta tattacttaa aattacgaag ggacattttt aaatcatgcg
     4201 tatcttaaaa gagtccatta ttgtggcatt tgcctttgtt ggtgttgtcg ttggtgccgg
     4261 ctttgctact ggtcaagaaa ttttccagtt tttcacaagt catggcgcat atagcatttc
     4321 aggcattatt gtaacaggac tattgattac tttaggtgga atggttgtca tgcatacagg
     4381 tcatcatcta aagtccagaa atcattctga ttcaattaac tatttcttat acccctctat
     4441 tgcaagaggt tttgatatta ttttgacaat gtttatgttg tctttagcta ttattatgac
     4501 tgcaggtggt gcgtcaacca ttcatcaaag tttcaactta ccgtattggc tgagcgcact
     4561 catattagtc gcctttattt tagcaacact gtttctaaaa ttcgatcgtt taattgctgt
     4621 gcttggcggt gttaccccat ttttaattgc gattgtcatt atgattgcgg tctactattt
     4681 cacaacaagt catcttgatt ttactgccgc taataatgat gctcagattc ataagcagaa
     4741 atcattatca cctggatggt ggtttgatgc gattaactat gcaagcttgc aaattgctgc
     4801 tgccttcagc ttcttatcag tgatgggtag taaagttaaa tatcgtgact caacgttata
     4861 cgggggcttg attggcggtt taatcattac atttttactc atgatgatta acctaggttt
     4921 aatttctcaa ttcgataaaa ttaaacacgt cgatctacct acattaaaat tagcgacaca
     4981 aatgtctccg tcaattggta ttattatgtc tgtcattatg atacttgtca tctacaatac
     5041 tgttgttgga ttaatgtatg catttgcgtc acgtttcagc gttccattta gcagacgtta
     5101 cttcatcatt attattacaa tggctgtcat cacttatatt agtacattta tcggtttcat
     5161 ttcattaatt ggaaaagtat tccctattat gggattgttc ggtttcatct tactcatacc
     5221 tgtactctat aaaggtttaa ttaagcgtat taccggcaaa tctcatatcg attaaaatat
     5281 ctatactatt gtaaaattta gtacaacact tcaaaatcaa aaaagcgggc agatgaaaag
     5341 taaaaatctt ttccatctgt ccgcttctta atagaactgt tatttcccaa gtatttgttg
     5401 accagccgtt aatccagttt ctgtaatttc atgaccattc acccaaactt cttcgactac
     5461 tgccccacgt gtcttaaaca agttaatgac ttgttcactt gcagctaatg gcacaattgg
     5521 atcatgtttc cccatagaaa gcaacacact tacatctgat aaatcctttg ttgacgttac
     5581 ttcaactggg tataacggtg catataacaa tgctttttta aatggtgctt ccgaacgcaa
     5641 cattaagtta atcgctatat ttgatccatt tgaaaatcca acaagtactg ctttttcaat
     5701 atcaaattca taacgttcag cagcttcttt aatgaacgct aacaattctt gtccacgaaa
     5761 ttccaaatct tcttcatcat aaacaccttc accaagacgt ttgaaatacc ggttcatccc
     5821 attttctgaa acttgtcctc taatacttaa caagtgataa ttttcattca atgcttcgcc
     5881 taatggcaat aaatcgaatt catcgccacc tgtaccatgc aacaatatta gcgttggcgc
     5941 acctttttgt ccttcacgaa aaatatgttc catcacaaat cctcctcatt ccaattaatc
     6001 atgttaacgc ttagtattaa atggtcttat ttctgattct atatactctc ttttattttc
     6061 taaaaatggt ggcaatgata aaccttcacc taatgtttca tacggttcat cttccataaa
     6121 tcctggtcca tctgttgaaa tttctattaa aatatgcccc acacgtgcat ataatgcttc
     6181 aaaatagaaa cgattaacga tgcctgagtt gttaatacct acctctttat atttcgttgc
     6241 ccacgcttct attgcatcat gatctttcac acgaaatgac acatgatgta cctcaccata
     6301 accttgacgt gctgctggcc ctttatcatc ttttattaag attacctggc caccattgcc
     6361 tccttcgcca acttcaagta atgcgacatt atcttcatgc gcaatagttg tcataccgta
     6421 aacagtctct aaaatatttt taaagtcgtc aaaataactt actttaattt caatgggtcc
     6481 taatccataa atcgctttat ctactggaac cggtccattc ttccaaggta caccaggtgc
     6541 taccccttca tttaactcat ctgaaattaa ttgatacact tggccatcga cttcttcaaa
     6601 tggcaacact tttttaccaa ataattcttg aataccttcg tgtttaacac caaattcatc
     6661 aaagcgctgt tcataataca ttaatgcgtc atcgttaggc actctaaaag acggtcttgt
     6721 aatggaattt gttcctgcct gcccttttgt aatatttgga aaatcaaaga acgtcatgtc
     6781 tgtacctgcc gaacctacat catctgcaaa aaaagtatga tacgtataaa tatcatcttg
     6841 attgactgtc tttttaacta aacgcatgcc tagtacttct gtaaaaaatt tataattacg
     6901 ttctgcatca tctgtcattg ctgtaacatg gtgaatacct aatagttcat gattatttgc
     6961 cattttatat accctctttg tcttttagca tatttatctc gaaaccgaga tattttaatg
     7021 taaattttta gtgcacgcct tacacttctg tagattgtgc acttaatttc ttaaacgctt
     7081 gttgtaagat tgttaattca tcctttgtta acacatcaaa cgcttttgtt aatgtctcag
     7141 catgcttagg gaaaatatct gccatttgac tttgaccttt ttcagttaaa caagccatat
     7201 atacacgttt atcatcttta tccttttcac gtgtaatcca acctttgtcc tctaattgac
     7261 ttacaacata tgaaatgctg ctacttgcaa ttaatacgcg gtctctaata cgttgaattg
     7321 gttgcggacc tttattataa agcaactcga gcactgcaaa ttcagtaata tttaagccat
     7381 atcgctttac gtcttctttt gtaatttgct ctaatgtgtc taacgcccta ttcattccga
     7441 caaagacatt gagcgattgt ttcgttcgat ccatatcatc accaactttt taatttattt
     7501 cgatttcgag ataaattata tagctaaatg aatttaatgt caagatacgt gttttcatct
     7561 tcatttcaaa ctgcgcttaa taaaaacgac tacataaatg tggcaatgtc gtcacatgac
     7621 tgtcatgcac ctttgacttc gtttaagtta aaatagaata gggaaatact ataatcaatc
     7681 atgataggag ttgatatgta tgagtaacct tgaaattaaa caaggcgaga acaaattcta
     7741 tattggtgat gatgaaaatc atgctttagc tgaaatcaca taccgttttg tggataataa
     7801 tgaaattaac attgatcata caggcgtatc tgatgaactc ggtggtcaag gtgttggcaa
     7861 aaaactagtt aaagcagttg ttgaacatgc tcgcgaaaat catttaaaaa tcattgcctc
     7921 gtgttcattt gccaaacata tgttagaaaa agaagattca tatcaagatg tatatcttgg
     7981 ttaaataaat tttgcgatgg tgcaactgtg ccatcgcttt ttatttgtac gaatcaatat
     8041 aaaattgcaa aacgacaagt tattttgcat ttcaaacttt atgctatcaa ttcattttct
     8101 tttctggtat actttttata ctaacttata gaaaaataga aaacgaggcg atgcataatg
     8161 gcatttcacg ataaaacagc aacacaagtg acaaacattg aactgaacgt aagagactta
     8221 gacttaatga caacatttta caaaaacatt ttaggactat ccgttaaaag ttctgacgat
     8281 aacacaaccg tattatctgt tgggactggt ggccatactc taacgttaca tttattagaa
     8341 gacggccgtc agacttcccc acgtgaagca gggctttttc atatagcact tttattacca
     8401 actactgagg atctagctaa cttcttatat ttcgtggcac aaaaaacatg ggcatcggcg
     8461 ctggtgatca tttagtaagt gaagctttat atttcaacga ccccgaaggt aatggtattg
     8521 aagtctatcg cgatagacct tcatcttcat gggaatggca aaatggcaaa gtaaaaatgg
     8581 atactttaga agttgatagc caaactttat taacacatcg tactgacgaa ggttggcaag
     8641 gaatgccagc aaaaggcatg attggacact tacatttaaa aacacatgat ttggacgcag
     8701 catatcaatg ttacattgaa caattaggat tccaacatgt gtctgatttt ccacgtgcgt
     8761 tatttatgtc aacgaatcat tatcaccatc atattgcggc taacacttgg caatccaatc
     8821 aagcaagaca aaacaatgca caaagttatg gattaacaca tgttgatatt tatcaacccg
     8881 acgcctcaac acatacattt acggcaccag aagggtttga catcacagtt cattcaaata
     8941 ctgaccttgt tccagaaaaa taatcatcca tactttatta atacaatttt ttcacgcaaa
     9001 agcccgcgat tcatttatat gaacgcaggc ttttattatt gctaactttt taattatata
     9061 tatttagaca tgctgtattc tatttattct tttggtccaa cccattcaat aacatcttca
     9121 tatgcttggc gtgttttatt tttcggtgga tcttgttgtc tgtagccaaa tgcaaccatc
     9181 actgataaac caaattgctc agtatctaag atacctttat ttgctaaaat gtctgtcact
     9241 gtatccagac taaaaccttc catcggacat gaatcaatac ctaacaatgc ggctgtcgtc
     9301 atcatattgc ctaatgcgat atacgtttgt ttacttgacc agtcatacaa ggcttgatca
     9361 ttatcagaaa tatggaaatc tgcttggaat gcatcgaatt tttgttcaac agctgggatc
     9421 gtttgtgcct catatttttt aatatctctt aacatatgtt gtacatacgg tgatcttgac
     9481 gttacatttt tacgcgcaaa aattagcaca aaatggctcg ctgtatctaa ttgttttgct
     9541 gcgccccaac tgtgcgcttt taattcatca cgtaacgctt gatcttgaat cacgacaaac
     9601 ttccaaggtt ctaacccaag agaacttgga gacaatctac ctgactctaa tattgtttca
     9661 aaatcttctt tcgaaacttt cttttgtgga tcgaattgct tagtcgcatg tctgaaatga
     9721 aatgcatcca taattgtttg attcatattg ctcattgaac gtagcctcca atttttatat
     9781 ttattactat acaaattata cttcatgttt taaaaagact aaagtaatcc gtttgcaatt
     9841 cactataact tgtgcacatg ttataagttt tctgcaataa aacacacttt tgaattttgg
     9901 aaaatttagt gttataatat atttgcgata tttttcacaa ttaaattgtg gcgtgatttt
     9961 aatgtacata atctttaatt tcactcattt acttttcaat cttttaaaag cgaggttttt
    10021 aataatgaca aaaattatgt tctttggtac gcgtgattat gagaaagaga tggcattaaa
    10081 ttgggggaaa aagaataatg tcgaagtaac tacttctaaa gagctattat caagtgctac
    10141 agtcgatcaa ttaaaagatt acgatggcgt aactacaatg caatttggta agttagaaga
    10201 tgacgtttat cctaaattag aatcttacgg tattaaacaa attgcacaac gtactgctgg
    10261 atttgatatg tatgatttag atttagctaa aaaacacaac attgtgatat ctaacgttcc
    10321 tagttattca cctgaaacaa ttgcagagta ttctgtttct atcgccctac aattagtgcg
    10381 tcgcttccca gatattgaac gccgtgtaca agcacatgat tttacttggc aagcagaaat
    10441 catgtctaaa ccagttaaaa atatgactgt tgcaattatc ggtacgggtc gtatcggtgc
    10501 tgctactgct aaaatatatg caggatttgg tgctacaatt acagcttatg acgcttatcc
    10561 taataaagat ttagactttt taacttataa agatagtgtt aaagaagcta ttaaagatgc
    10621 cgatattatt tctttacatg ttccagcgaa caaggaaagc tatcatttat tcgataaagc
    10681 aatgtttgat catgtgaaaa aaggtgcaat tttagttaac gcagcacgtg gtgcagtcat
    10741 caatacacct gatttaatcg ctgcagtgaa cgatggtact ttgttaggtg ctgcgattga
    10801 tacttatgaa aatgaagcag catacttcac aaatgactgg actaataaag acattgacga
    10861 taaaacatta ttagagttaa tcgaacatga aagaatttta gtaacaccac atattgcttt
    10921 cttctctgat gaagcagtac aaaaccttgt tgaaggtggt ttaaacgcag cattatctgt
    10981 cattaacact agtacatgtg aaacacgttt aaattaatat atatttaaaa gcaaaggcgc
    11041 gccatgtgcc tttgcttttt attttgtata tctgtataaa aaccagtttg attaaaatcc
    11101 gctttatttt atcatctatt caaatgattt tagtgcgctt attttactat ggcattattg
    11161 cctaacgtgt ttgagatata ttaatatttg attattcgta ttcaaagttt tcatttaaca
    11221 tgtatttagt atcataatag ccgcttcatt gatgatgtac tacctacttg ttcaatactt
    11281 cttgttcgat aatcgttaaa acaccctgct tatcattact tggagcgaca gcagaggcaa
    11341 tcttgaacaa ttcttcatca tgactatttt ccatcacata actatgctta gcgaacgcaa
    11401 gcatatcttt atcattattc gcatctccga aggccatgag ctctgaagaa gacattcccc
    11461 atttatctaa caatcgtttt aatgcctgcc ccttagtcat gtttggcata ataatatcaa
    11521 tgctatcgtg cccacttgag acaagtttaa tatcattgct gaattgcgtt gctacttctt
    11581 cgtcaacatt tggatgcgtc tcacgattaa tattaaatgc tattttgaca taatcatcct
    11641 caggtaattg ttgtagactg tcaatttctt ttaactgatg ataataaaat ctcgtatctt
    11701 ctttaaatgc ttcagaagta tgttttaaaa tatacgcgct tttcaaacca cagatgacga
    11761 gttgatcaat actttggttc atatttaaat aatcgacaac ctgttgaaac acctgacgat
    11821 taaagctttt ataattatat aactcattgc cattataaat aactgcacca ttttcagaaa
    11881 taaaatacat atctctatcc ccgaaaatag acttcaactt cgcatattga ttgccactcg
    11941 cagcaataaa tgtaatatct ctatttctaa gttcagtaaa aatcgcttca aatcttgatt
    12001 tatcgtatgt ctttttcgaa tctaaaaacg tcccgtccat atctaccgca atagccttaa
    12061 ccatgattat cctcctcatt gctcttttag tcgtcttcat tatatccatc tcatgacaag
    12121 caatgaagtt tgtcatacca caatgaacct atattcacat tttgaaattg ctttcatata
    12181 tgcaaaacac attacactgg atatagcatt cagaggcgaa cataaatttc agaatattgt
    12241 aaaaatataa cgata
//
LOCUS       DAMZLE010000073        12233 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMZLE010000073 DAMZLE010000000
VERSION     DAMZLE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.073"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            271..2420
                     /locus_tag="POB92_001729"
                     /pseudo
     CDS             271..2420
                     /locus_tag="POB92_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(2648..3463)
                     /locus_tag="POB92_001730"
     CDS             complement(2648..3463)
                     /locus_tag="POB92_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6579218.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(3456..4899)
                     /locus_tag="POB92_001731"
                     /pseudo
     CDS             complement(3456..4899)
                     /locus_tag="POB92_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(4871..5464)
                     /locus_tag="POB92_001732"
     CDS             complement(4871..5464)
                     /locus_tag="POB92_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6579219.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            complement(5728..6108)
                     /gene="blaI"
                     /locus_tag="POB92_001733"
     CDS             complement(5728..6108)
                     /gene="blaI"
                     /locus_tag="POB92_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDI6579220.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(6098..7855)
                     /gene="blaR1"
                     /locus_tag="POB92_001734"
     CDS             complement(6098..7855)
                     /gene="blaR1"
                     /locus_tag="POB92_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDI6579221.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSLFKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAETIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNVPFFN
                     KNILSHSFNGKKSLLKRRLINIKEANLKKQSKLILIFICIFTFLLMVIQSQFLMGQSI
                     TDYNYKKPLHNDYQILDKSKIFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPNSTYK
                     IYLAMFGLDRHIINDENSRMSWNHKHYPFDAWNKEQDLNTAMQNSVNWYFERISDQIP
                     KNYTATQLKQLNYGNKNLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGK
                     NAELISEKILKEMGVLNGQ"
     gene            7962..8807
                     /gene="blaZ"
                     /locus_tag="POB92_001735"
     CDS             7962..8807
                     /gene="blaZ"
                     /locus_tag="POB92_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDI6579222.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKTGDTLIKDGVPK
                     DYKVADKSGQAATYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            9176..9709
                     /locus_tag="POB92_001736"
     CDS             9176..9709
                     /locus_tag="POB92_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6579223.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNE
                     SKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEV
                     NITRQTIYRIKNDNDLI"
     gene            complement(9891..10457)
                     /gene="qacR"
                     /locus_tag="POB92_001737"
     CDS             complement(9891..10457)
                     /gene="qacR"
                     /locus_tag="POB92_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator
                     QacR"
                     /protein_id="HDI6579224.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFK
                     TKENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEF
                     YTEYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIV
                     TFTHEQNINERIKLMNKFSQIFLNGLSK"
     gene            10673..12178
                     /gene="qac"
                     /locus_tag="POB92_001738"
     CDS             10673..12178
                     /gene="qac"
                     /locus_tag="POB92_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound
                     efflux MFS transporter"
                     /protein_id="HDI6579225.1"
                     /translation="MKKRWTALVVLAVSLFVVTMDMTILIMALPELVRELEPSGTQQL
                     WIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFAESAEFVIAIRF
                     LLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPIIGGALLEQFSW
                     HSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIGLVWSIKEFS
                     KEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTIAAFMTMFA
                     MASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPKIVLPSGI
                     GIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKAGNAAAV
                     EESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGAVEVAK
                     ATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
BASE COUNT         4226 a         1745 c         1743 g         4519 t
ORIGIN      
        1 ttaaaaatca taatacgaag ttaaataaaa aatctcacaa taaaaatgtt catcatgctc
       61 gtttgagaat cctcctaata ctctatcaaa gtcttcctat aatttgtgtt attacttctt
      121 cttgtatgat agcgctaata taactaattt gagttttgac aatatttaca tttaaaaatg
      181 agtttgtgct atattgtttt tgaaatgtaa tattgattat aatgttttgc ttgtgaagat
      241 atcattataa aataagattg gggcttatat atgaagttta ttaaacaata cgatgagagc
      301 gactgtggac cagcatgttt agcaatgtta agtcatcact ttggaaaaaa aacattaata
      361 tctagaatta gagaatggac aaaaactgat aaaaacggta catctttata tggtatgatt
      421 gaaggtggga aacaattagg cattgaatta actggggtta aagctaaaaa agttaatgat
      481 atttctaata ctgatttacc tctgattgca catattacaa atgagcaagg atatatgcat
      541 tttattatca tagaaagaaa aactgaaaac aaaatttata ttattgatcc tgcaaaagga
      601 aaagaaaaaa ttagttttac ggaatttgaa aaaaatggac tggtatactg ttgcttgttc
      661 agaatgatgt ttcatatggc tatgataata acctccctag caaatataag ttgctcacaa
      721 aaatattaaa aagtaacaaa attcctattt ttaatatttt tatgttatca ctgttcataa
      781 atatattggg tattataggt gcattttact ttaaatatct tgtggatact ataatacctt
      841 ctcaaattat acaaaattta catatattat ctatagctgt actatttata tacttaatta
      901 atgcattggt ttctttaatc agatatcaag cttctttaaa cttaagtcta aaaattgata
      961 tgaattttat gaaagattat tattatcacg ttttaaattt accaataaaa ttttacgaaa
     1021 caaggaaaag tggagaaatt ttatcaaggt tttctgattt atctcatata agagaagcac
     1081 tttcttcagt tactataaca ttattagtag acacattaat ggttttagtt ggtggaactg
     1141 ctttattctt tcaaagtgcg ctgttatttt atataacatt aattctgatt ccaatatatt
     1201 taattatagg attatcattc agaaaaatat taagcaaata taatagacta gtgatggaac
     1261 aagacgctac tttaagttca tatcttgttg aaagtttttc aggttattca gtaataaaaa
     1321 gtttcggagc agaaaatgaa atatttaata aaggagttaa tcactttaaa aaattaatca
     1381 gaagattgta taaacttagt ttttttacta atatgcaact aactattaac acttttatga
     1441 aaatgacaac gactttaatt atcctttgga ttggatcatt tcttataata aaaaatcagt
     1501 tgactttagg agaactttta acgttcaatg ctttagtaat atattatata gatccaattg
     1561 agagattaat aaacctacag ccacaaatac aatcagcaat tgtcgctacg caaagatatt
     1621 tagatataac agatattaaa actgaaaatg aagaaagaaa taataataat acattaatag
     1681 attctacttt cacttttaca aataaactgg aaattaggaa tcttaacttt caatataatt
     1741 ttaaagaaga agctttgaaa aacattaata tatcaatact caaaaatcaa aaagttgcta
     1801 ttataggtga aagtggttca ggaaagtcta ctatagcaaa attaattgat aattttcata
     1861 taaattatga aggtagtata acgcttgata atacttcaat aaaagaaatt cccaaaaata
     1921 aactgagaaa tttgattagt tttgttacac aacagaattt tatttttgga gctacaatta
     1981 gagaaaattt gactattgga ttaaatagaa aagtctctga ttctgatata tataaagctt
     2041 gtaaaatagt ttgtgcatat gattttataa ttaaccttcc acaaaaatta gatactcaat
     2101 tacataatgg aggttctaat ttatctggtg gacaattaca aagaatagca ctagctagag
     2161 caatattaaa agattccgat atattattat tagatgaagc taccagttct ctagatgcat
     2221 ctactgaaaa aaatatatta gagaatatag atcttcattt atcaagcaaa acaatcattt
     2281 taatcacaca taaattgact aatgttaaaa atgctgacaa catttattta ttaaagtatg
     2341 gtcaaataat tgaagaggga actcataaat cattaatcaa taaaaaaggt gaatactata
     2401 atctatggaa aaatcaataa ggttaatgat ttactttaac cattcttaat gaaagattca
     2461 cctctaataa atataaaaga aaaaatctat atttattttt agtaagtttt aattatagat
     2521 atgtatactt tgagtacacc ctaatcatac atatataatt gttgtcactt tactcgatat
     2581 atgagaatga ctttacctaa taaatgagaa aagcaacgca ctatattaaa atgcgttgct
     2641 ttttctatta tcgaatacct ataactaaac tatctctagc tgtttctaca acttctgtac
     2701 ttattttatc taaaccattt atatccataa ttctgtctat ctgcgcaaat aaacgatgaa
     2761 tcagcctaaa attccctttt gtaattttaa taatcgtcgt tattgcctca taatcagtaa
     2821 agtcttcgag ttttaaatca aatcctaagt cttgacactt atattctaat atatgatgcg
     2881 tctcatcttt acttagatta tcaaattcat gcgcaaaacc tatacgcgaa tatagttgag
     2941 gataacgaga tagttttttc tctatacccg gcataccaat aaatatcatt gctaaattat
     3001 gttcatcata aatagctctt aattgctcta aatgttgcac tttaaggcga tcaatctcat
     3061 ctactataat taaatcaata tatttaaaca tatcactata atgtttacta tgttcatgtc
     3121 catacttatt tacaacatgt aatttttgac ccaaatcaat gattgcagta atactatata
     3181 aatcattcct taatttagtt tgtttttctg ctggtgcatt ataaaatatt ttatccaccg
     3241 atagtatgtc atcggtcgtt ttactagcta aatcttccca acctctatgg ttaacttgtt
     3301 tttcaatagt atcccaattt gtgtaatacc tagacgataa agtttttccg acaccaggtt
     3361 gaccataaca tataccaata tactgatatt taatacaagc atcacaaaat tcagcaaagc
     3421 gtttatactc tttcgtttca atgaaatttt gatttttagt cattacgata tctctttaat
     3481 tttttgacgt ttttagtcgt ttcttgaggt gaataaccac aatttttttc ttccttaatt
     3541 aattccgtgg tacttggact agcaatattt tgttttaagt gttttcttct ctgactacga
     3601 gcgtgctgta tgtcttttat atctatggag taatcagcta tttctggaca tatagcagta
     3661 caaagaaatt catctctata aaacacacgt atttcagcca tatcatttgg gttataacga
     3721 atcagtacat attcacctac ataagcttct agattagtat tactgtatcg aaaaccttga
     3781 aaatgaatgc catctgaatg aacttttcta gatttaggta tttccaacaa taataaatca
     3841 agttgttcta agctacttgg catattaggg aaaaagtgat tactattcca atgactgata
     3901 ggcgtggtat gaatagtact atgttctttt tgattataat cttcaattaa aaagtaacgt
     3961 aatttttctt caaaattgtg aaaatttatt agattactag gagtatcatt attgtttata
     4021 tatccaggca gttgttctaa gaaagtttga ttaactgtct gaaagaatcg ttctattttt
     4081 ccacgaccac gaggaacacc aactttcgaa aacatcaagt taattttcaa atcaatagcg
     4141 acttgttcca tatgatgtga agtaaagtca cttccatgat ctgtgtagaa tttttcgggt
     4201 ataccacaca ctggccaatt ggtatcattt ttattccata ttgcttggtg tagtgttaat
     4261 gctgtgtttt gagcattggg gggcatcaaa acttaagaaa tagcctgcaa ttgcacgact
     4321 atagtcatcc ataataatag ttagccatgg tctattgata ttaccttttt ggtctaatat
     4381 aaagatatct agcaaagtat ggtctgcttg ccatatttca ttgggtctcg aggattctct
     4441 aatttgtatt aaatcgtact tattttgata gtatttttca ccctgatgag agaaatcaat
     4501 aacagactta ggcatcgctt taataacact ataaatttgt ttataacttg gtatatcaaa
     4561 attattttct ttacagtaat tagtgatttt cctatgaatt gtggctatac tatttctttt
     4621 attcataagc gcaaggcgtt caatttcatc aattatcttt ggttctattt tacgtgttcc
     4681 tttatcagct cttgtcgcat agattagtcc tttaagtcct tgttctttat atgacttatt
     4741 ccacctataa agggtgctta acgcaatccc tttactttta gaaatacttg ataaagattg
     4801 ttcgcctagg ataaaaggtt ttataatatt gtacttttga gtagcttttt tacgtttggc
     4861 ttcagaaaaa ttagttagat acttctcctt gtttttcatc ttccttgtcc tccaatttat
     4921 tgaggtaacg ataaattgtt gtacgtgaaa catgccactg ttctgcaatt gtcttaattg
     4981 gcgtgccatt atcataaagg gtcttaagta agtttaaatc tttttgattt aatttttctg
     5041 gtctgcctcc atatcggcct ctagcacgtg ctgcaattcg gccagctgaa gaacgctcta
     5101 aaattaaatt acgttcaaat tctgcaaatg ctgcgaataa atgaaataat aattgtccag
     5161 ttgaacttga tttatccatt gttatatttt cttctaaact atggaaactt acacctcgtt
     5221 cgttaagctc attaactaat gtgattaaat cctccatatt acgtcccaat ctatctaatc
     5281 tccaaacaac aatcgtatct cctgacctcg caaattcaat tgctttatct aaaccaggtc
     5341 ttttactttt tgaaccactc atatggtcat taaatatctt ttcacaacca tatgtattta
     5401 atcgatcctc ctgtaaattt aaattttgta atccagttga aactcttgca taacctattt
     5461 tcaaaataca aacctcctat atacatcttc agtgtaccat aacttaatta caaggtcttt
     5521 tatgaacata gattttttgt tgggtttttg aacacataat cctatgtaat tcttacttat
     5581 ttagacataa aagaagagtg tacagaaaat ggttattttc tgtacactct catctttaat
     5641 aatatatttt aaatgttatt aacaaaacta atttaataag agtcaagcat agtttacata
     5701 tgtattatgt ttgaaacata tctctaatta ctttttacta atatcattta aaatgtctcg
     5761 caattcttca atttctttgt tatttaattc ttcatttttc gcaaaattca gcactaaact
     5821 tttcatgtcc cctccataca gtttattaag aaaggtttta gcagttttca ttttaatatc
     5881 gtcttcttta atatttgatg agtaaaaata aatattctct gatttgtatc gttttataat
     5941 ctctttttta tatagtcttg tgattaatgt tctaatcgtt ttatcgctaa cttctttata
     6001 tttttgaatt tcaactacaa tttcattagc tgatactgat tttttacccc atattatatt
     6061 cataacatcc cattcagcca tagatatttc aacttgctta ttggccattt aaaacaccca
     6121 tttcttttaa tattttttca ctgattaatt cagcattttt cccagatggc tttccatctg
     6181 ataaatgtgt agcaaaataa tacttatcat gatttgtaat tacgtaacct acaaaccacc
     6241 cattattata cttcccgttt actatacctg tacctgtttt cccatacagt tcatactttt
     6301 catttttctt aatcaataat gaagaagata attgattctt tgctttttta ctaaaatggt
     6361 tattttgttc catcatattt ttaaaaacta ttacttgttc aagattagat attttcaaac
     6421 tatcttccat ccaatagctt ttataacttc ccaaattttt attaccataa tttaattgct
     6481 tgagttgagt cgcagtatag ttctttggta tttgatcgct aatacgttcg aagtaccaat
     6541 taactgaatt ttgcattgct gtatttaaat cttgttcctt attccaagca tcaaaagggt
     6601 aatgcttatg attccatgac atacgtgaat tttcatcatt tataatatgt cggtcaagtc
     6661 caaacatagc taaataaatt ttataagttg aattaggaga ataccttttt ctactttctt
     6721 tttcgttata aatatagtat ttgtctttct tcatgctgta catgacgaaa gatccactat
     6781 ttgaaccaaa aattttactt ttatctaaaa tttggtaatc gttgtgtaag ggctttttat
     6841 aattataatc agttatggat tgccccatca aaaactggct ttgaattacc atgagcaaaa
     6901 aagtaaaaat acaaataaaa attagaatta atttggactg ttttttcaga ttggcttctt
     6961 ttatattaat taatcttctt ttgagtaatg actttttacc attaaatgaa tggcttaata
     7021 tatttttatt aaaaaatgga acatttaata cggagtccat aatagattcc gcataagttt
     7081 taaattcatt tttgttaata ttattaagga caaatctatc ggcttctact tcattgtcat
     7141 gaattatctt tctttttaca atatgtacta atggattgta actcataata atagagaaga
     7201 tattaaagat aattaaatgt aaagtatctc tatttttagc atgagcatat tcatgcaata
     7261 ttatatattt tagtcttttg tcaattacac ttttaaaata tgaactagga atcaaaataa
     7321 tatatttccc ataccaaaaa gttattggag attgaatagt ctctgctttt cgaatcacaa
     7381 tatttttttt atattgatgg ttgaaaagta tcgtatctat tttatttttt tcattttcgt
     7441 ttagataaag tgactgtttc tttaaatatt taagatataa taaggctttc aaaaatttaa
     7501 aacttaaaat aataactaaa actatccaaa taactgtgca gatattatca attgaatccc
     7561 aattaaactt atggatatct gttgcgaact cttgaatagg tttggtggta tttatgttat
     7621 ggttcaagtc gtgtgactta ctttcaactg tgggcgcttg attattcaca ttattaaatt
     7681 taaaaagaga gaatttaata ggaatgaaag gaattaatcc tgcaagaaga gttagatacc
     7741 aaactttata atttaacata taattaaaat agcgttttaa tatgtaccta aaaaataata
     7801 acaatagaaa tataaaacaa aagcttacta tgctcattat taataactta gccatttcaa
     7861 caccttcttt caaatattta taataaacta ttgacaccga tattacaatt gtaatattat
     7921 tgatttataa aaattacaac tgtaatatcg gagggtttat tttgaaaaag ttaatatttt
     7981 taattgtaat tgctttagtt ttaagtgcat gtaattcaaa cagttcacat gccaaagagt
     8041 taaatgattt agaaaaaaaa tataatgctc atattggtgt ttatgcttta gatactaaaa
     8101 gtggtaagga agtaaaattt aattcagata agagatttgc ctatgcttca acttcaaaag
     8161 cgataaatag tgctattttg ttagaacaag taccttataa taagttaaat aaaaaagtac
     8221 atattaacaa agatgatata gttgcttatt ctcctatttt agaaaaatat gtaggaaaag
     8281 atatcacttt aaaagcactt attgaggctt caatgacata tagtgataat acagcaaaca
     8341 ataaaattat aaaagaaatc ggtggaatca aaaaagttaa acaacgtcta aaagaactag
     8401 gagataaagt aacaaatcca gttagatatg agatagaatt aaattactat tcaccaaaga
     8461 gcaaaaaaga tacttcaaca cctgctgctt tcggtaagac tttaaataaa cttatcgcaa
     8521 atggaaaatt aagcaaagaa aacaaaaaat tcttacttga tttaatgtta aataataaaa
     8581 ccggagatac tttaattaaa gacggtgttc caaaagacta taaggttgct gataaaagtg
     8641 gtcaagcagc aacatatgct tctagaaatg atgttgcttt tgtttatcct aagggccaat
     8701 ctgaacctat tgttttagtc atttttacga ataaagacaa taaaagtgat aagccaaatg
     8761 ataagttgat aagtgaaacc gccaagagtg taatgaagga attttaatat tctaaatgca
     8821 taataaatac tgataacatc ttatattttg tattatattt tgtattatcg ttgacatgta
     8881 taattttgat atcaaaaact gattttccct ctattatttt cgagatttat tttcttaatt
     8941 ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag atgaatagtt
     9001 taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt
     9061 ttctcattta taaggttaaa taattctcat atatcaagca aagtgacata attgttgaat
     9121 ttatagtaac tgaaacgtat gattagggtg tatattaatt atgagatgga aatcaatgat
     9181 tgttggctat gcgagagtat catcgaacga tcaaaattta gaaagacaac ttgaaaattt
     9241 aaagacgttt tgtgctgaaa aaatatttac agaaaaacag tctgggaaat ctattgaaaa
     9301 tagaccagta cttcaagaag cacttaactt tttgaggatg ggagatcggt ttgtagtaga
     9361 atccattgat tgtttaggtc gtaattatga tgaagtggtt ggcaatccat tactggataa
     9421 gtttatgaaa gatttaatta ttcaaatatt agcaatggtt tcagaacaag agagaaatga
     9481 aagtaaacgt cgacaagcgc aaggaattca agttgcgaag gaaaaaggcg tatataaagg
     9541 gcgcccctta ctttattcac caaatgctaa agatccacag aaacgtatta tttatcatcg
     9601 cgttgtagaa atgttagaag aaggccaatc aattagtaaa attgcgaaag aagtaaatat
     9661 aacaaggcag acgatttaca gaattaaaaa cgataatgat ttgatttaat aaactttgtt
     9721 tcaatgcaac tgagagcttc tcacagcgtc tcaaaacaga tgattataat ctattatgag
     9781 tttttaataa taagacaaga aaagagagcg taaatatgct ctctcttttt ctcattaacg
     9841 aacgtaattt agaaataata tttattggta tttcaagaat atatatataa ttatttacta
     9901 agtccattta aaaaaatttg agagaacttg ttcataagtt taattctttc attaatattt
     9961 tgttcatgtg taaaggtaac aattccatta acagcgtttg ctgctatttt actaacagca
    10021 ttaacatcat taatacacca ttcgccattt aaattaccct ccttaaaaat tacatgataa
    10081 gcatctatat atttgttttc taatttattc attttttcat taatgctatt agttttataa
    10141 tattcagtgt aaaattcaat tattgcgttt tgaagtggat aatagtattc ggttgttaaa
    10201 gatagttcat tatataaata aaatttttct ctattagttt tacatttgat ttgttccttt
    10261 ttccactgtt cttgccattt agattcttct atatttaaaa tttctaaaaa tagattttct
    10321 tttgttttaa agtgataata aagattccct ttactacttt ctgataattt aacaatttct
    10381 ccagtagtag tggcattata tccatttttt ataaataatt cctttgcgac acctagtatt
    10441 ttatctttca agttcatcac gcctttaatg agtattaagt actatcaatg atagcacaaa
    10501 aaattcaaag aataattgta taacacaata cagccattta aatttcgcaa gattttttgt
    10561 tgtaatatgt aaaaaaatag attataatcc ttatagaccg atcgcacggt ctataaggat
    10621 tggagggaac ttaaatgatt tcatttttta caaaaactac tgatatgatg acatcaaaaa
    10681 aagatggact gcactagtag tattagctgt tagtttgttt gttgttacaa tggatatgac
    10741 aatattaatt atggctttac cggaattagt aagagagtta gagccttctg gtacccaaca
    10801 gttatggata gttgatatat actctcttgt tttagctggc tttataattc cattgagtgc
    10861 ctttgctgat aaatggggaa gaaaaaaagc attattaact ggatttgctt tatttggcct
    10921 cgtttcatta gctatatttt tcgcagaaag tgcagagttc gtaatagcta ttcgattttt
    10981 acttggtatt gcaggtgctt taataatgcc aactactctt tcaatgataa gagtaatttt
    11041 tgaaaaccct aaagaaaggg ccactgcatt agctgtatgg tcaatcgctt catcgatagg
    11101 tgctgttttt ggaccaatta tcggaggagc tttacttgag caattttcat ggcactcggc
    11161 atttttaatt aatgtaccgt ttgcgataat agcagttgta gcaggtttat ttttattacc
    11221 agagtctaag ttatcaaaag aaaagtctca ctcgtgggat attccttcta caattttatc
    11281 aattgcaggc atgattggac tggtatggag tatcaaagaa ttttcaaaag aaggactagc
    11341 agatattatt ccatgggttg taatagtatt agcaattacc atgatagtga tatttgttaa
    11401 acgtaattta tcaagttctg atccaatgtt agacgtaaga ctttttaaaa agagatcatt
    11461 ttcagctggt acaattgctg catttatgac aatgtttgca atggcatctg ttttgttatt
    11521 agcttcacaa tggttacagg ttgtggaaga actttctcct tttaaagctg gcttatacct
    11581 attacctatg gcaataggag atatggtgtt tgcaccaatt gcacccggat tagcggcgcg
    11641 atttggaccg aaaatagtgt taccttccgg aattggaatt gcagccattg gcatgtttat
    11701 tatgtatttc tttggtcatc cattatcata ttctacaatg gctttagcat taattttagt
    11761 tggagctggt atggcttcac tagcagttgc atctgctcta ataatgttag aaacacctac
    11821 atcaaaagca ggtaatgcag ctgctgttga agagtctatg tatgaccttg gaaatgtttt
    11881 tggtgtagca gtacttggta gcctatcttc tatgctttat cgtgtatttt tagatatttc
    11941 atctttttca tcaaaaggta tagttggaga tttagctcat gtagctgaag aatctgtagt
    12001 gggcgctgtc gaagtagcta aagctacggg gataaaacag cttgcaaacg aggctgtaac
    12061 atcatttaat gatgcttttg tagcaactgc tttagtaggt gggattatca tgattatcat
    12121 ttcaatagtt gtctatttgt taattcccaa atcacttgat ataactaaac aaaaatagta
    12181 ctaaacatat atttttagat attgtagatt agtttatttt taaaattagt aat
//
LOCUS       DAMZLE010000074        12223 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMZLE010000074 DAMZLE010000000
VERSION     DAMZLE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.074"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(172..1074)
                     /gene="cvfB"
                     /locus_tag="POB92_001739"
     CDS             complement(172..1074)
                     /gene="cvfB"
                     /locus_tag="POB92_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDI6579226.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRMDSKE"
     gene            1221..2822
                     /locus_tag="POB92_001740"
     CDS             1221..2822
                     /locus_tag="POB92_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6579227.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3689..4894
                     /locus_tag="POB92_001741"
     CDS             3689..4894
                     /locus_tag="POB92_001741"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI6579228.1"
                     /translation="MVTRSVLKYGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVAGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4958..5947
                     /locus_tag="POB92_001742"
     CDS             4958..5947
                     /locus_tag="POB92_001742"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6579229.1"
                     /translation="MTKLAVVGSTGLVGTKMLETLNRKDIPFDELVLFSSARSAGKEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5949..6836
                     /locus_tag="POB92_001743"
     CDS             5949..6836
                     /locus_tag="POB92_001743"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI6579230.1"
                     /translation="MTHLFEGVGVALTTPFTNNKINIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            6833..7555
                     /locus_tag="POB92_001744"
     CDS             6833..7555
                     /locus_tag="POB92_001744"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI6579231.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNIPVFF
                     SANMSYGVHALTKILAAAVPLLDEFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7582..8301
                     /gene="dapD"
                     /locus_tag="POB92_001745"
     CDS             7582..8301
                     /gene="dapD"
                     /locus_tag="POB92_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDI6579232.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8444..9595
                     /locus_tag="POB92_001746"
     CDS             8444..9595
                     /locus_tag="POB92_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6579233.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEDYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9600..10685
                     /locus_tag="POB92_001747"
     CDS             9600..10685
                     /locus_tag="POB92_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6579234.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLRETIQIRQLVPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10675..11940
                     /gene="lysA"
                     /locus_tag="POB92_001748"
     CDS             10675..11940
                     /gene="lysA"
                     /locus_tag="POB92_001748"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDI6579235.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
BASE COUNT         4247 a         1915 c         2249 g         3812 t
ORIGIN      
        1 aataattaat agaaatcagc ttttttacat tgcctaagaa cttaatgtcc cagcctattc
       61 gtttgaatta tttaattttt tactttgaaa ggaatgttta aaaaactact tcatctgtcc
      121 gatttaatat tgaataaaaa aacacgcatc ctacgacacg tgtaaaaatg attattcttt
      181 tgagtccatt cgactccaac cttttttagt taaagtgatt ttacctgttt ctatattaat
      241 aatcttctgt ttatataagt gaccgattgc acgtttgaat gaacctttac tcatattgaa
      301 tacttcttta atcgcttcag ggcttgattt gtcccagaat ggtaattcac catcatattc
      361 aactagtaaa tcaaagatga cttggccgtc atcgtctaaa cgttcatgtg caagtggtaa
      421 aaatgaacca tttaactcac ctttatcatt atgcccgata attctaactt gaacagattc
      481 acctaatctt ggttcagctt tacgttctga ttcatgtacg aaaattttgt aacctgattc
      541 gcttaataaa aagctaccaa ttcgtaatac gcggtaaggt ttggcttcaa tgacttcgtt
      601 ttttaaatta tcgtcgtgta caggtgtaaa catattttct acaacagatt cactcgctaa
      661 tcttccatac atatgattct cacggtcaat tcgtaatgtg actagcaaat gatcaccagg
      721 ttgtggccat agtgatttca cttttggtaa atcttcccat ggtactaaca cttcacgggg
      781 taatccaaca tctatacgtg ccccatcgcg atccgtttta agtactttag caaagtcata
      841 tttatctttc gtaatatcag gcatattttg agttgcaaat aattcacctg aacggtttgg
      901 ataaatgaag aaactatatt cttcacctac ttctaattca tcatcatcgt tcatttctga
      961 ttggtttaac tttacgtttt caccgtttgg tccttttaaa aggtaagttg agccttgtaa
     1021 ccctactact tcaaggaatt ctatagaacc tactatatct ttgtctaatg ccatgttatt
     1081 ctccttcgat tcttgtattc tttattatta taacatgtgt gagtcattct atcattacaa
     1141 cttatatgag gtcgaaactg tgacgataat atgatataat tcttccgtta gaatatcgaa
     1201 tgaaaaaaag gagaaaatgc atgttacaag taactgatgt gagtttacgt tttggagatc
     1261 gtaaactatt tgaagatgta aatattaaat ttacagaagg taattgttat ggattaattg
     1321 gtgcgaatgg tgcaggtaaa tcaacattct taaaaatatt atctggtgaa ttagattctc
     1381 aaacaggaca tgtttcatta ggtaaaaatg aacgtctagc tgttttaaaa caggaccact
     1441 atgcttatga agatgaacgc gtgcttgatg ttgtaattaa aggtcacgaa cgtctttatg
     1501 aggttatgaa agaaaaagat gaaatctata tgaagccaga tttcagtgat gaagatggta
     1561 tccgtgctgc tgaacttgaa ggtgaatttg cagaaatgaa tggttggaat gctgaagctg
     1621 atgctgctaa ccttttatct ggtttaggta tcgatccaac tttacacgat aaaaaaatgg
     1681 ctgaattaga aaacaaccaa aaaattaaag tattattagc gcaaagttta ttcggtgaac
     1741 cagacgtact attactggat gagcctacta acggtctcga tattccagca atcagttggt
     1801 tagaagattt cttaattaac tttgataata ctgttatcgt agtatcgcat gaccgtcatt
     1861 tcttaaataa cgtatgtaca catatcgctg atttagactt tggtaaaatt aaagtttatg
     1921 ttggtaacta tgatttttgg tatcaatcta gtcagttagc tcaaaagatg gctcaagaac
     1981 aaaacaagaa aaaagaagaa aaaatgaaag agttacagga ctttattgct cgtttctcag
     2041 ctaacgcttc taaatctaaa caagcaacaa gtcgtaaaaa acaacttgag aaaattgaat
     2101 tagatgatat tcaaccatca tcaagaagat atcctttcgt taaattcacg cctgagcgtg
     2161 agattggtaa tgacttatta atcgttcaaa atctttctaa aacgattgac ggtgaaaaag
     2221 tattagataa catttcattc acaatgaatc caaatgataa agcaatttta attggagata
     2281 gtgaaatcgc gaaaacaaca ttacttaaaa tattagctgg tgaaatggaa ccagacgaag
     2341 gttcttataa atggggtgta acaacatcat taagttactt ccctaaagat aactcagaat
     2401 tctttgaggg cgttaatatg aaccttgtag attggttaag acaatatgca ccggaagatg
     2461 aacaaacaga aacattttta cgtggtttct taggtcgtat gttatttagt ggtgaagaag
     2521 ttaagaaaaa agctagtgtg ctttcaggtg gagaaaaagt acgttgtatg ctaagtaaaa
     2581 tgatgttatc aagtgcgaat gtacttttac ttgacgaacc tactaaccac ttagacttag
     2641 aaagtattac tgctgtcaat gatggtctta aatcatttaa aggttctatc atctttactt
     2701 cttatgactt cgaatttatc aacacgattg caaaccgtgt tatcgattta aataaacaag
     2761 gcggcgtttc aaaagaaatt ccatatgaag aatacttgca agaaatcggc gttttaaaat
     2821 aaacataata tgtagaattt cttttcgaaa atctctgtgt tgaggcccga ccaacttgca
     2881 cattattgta agttggctta ctgtcacctt ctatattggg acttattccg caacttgtat
     2941 tgtttgtaga aattaggaat ccaatttctc tatgtgtggc accgacaagg attgaaaaaa
     3001 gctagctaca aagtcatttt cattcagtca actactgtca atataacatt gcagggccta
     3061 gggcattgat tcatgtccga gactatattc aatttaatag acattaactg ggcgtctcga
     3121 tgctaatatt tatagcatca aggtgtcctt tttattttat tttttagcac atcagcacag
     3181 tgaacacccc ttctctaaat tacaaataca gttatcaccg cattaaaaga gtacatttat
     3241 ttaatgtgca tattgagtat ggagtatgct ataagtattt tgaattttat ttttcaaaaa
     3301 gtctcattat caccaccttt taaaatataa gcaacgctat aacttgaatt ttcaggttat
     3361 tccaaaaaat ctaaattatc ggtttacatt catatttgtc atatgtataa tgaacacata
     3421 ccaatttaat aatattttga tgaggcgcat caatcatgag taaagtttag attactgtct
     3481 gctaacagct aaatttgaaa gggtgcgatg ccgaagcgat tataatagca gttataattt
     3541 gttggacttt ttggttaaga gctgagagtt tgtcattatt taaaaataat ggagtgcatc
     3601 acttgtatat agattaagag caagttcgca ttccgaactt gttctttttt tattattgtg
     3661 tgcccttcct aacaattagg aggattatat ggtaacaaga agtgtgttga aatatggcgg
     3721 atcatccgtc agtgatttta caaaaataaa aaggatcgct gaaatgttaa aggagcgggt
     3781 caatcaagat gaacaattaa ttgtcgttgt aagtgctatg ggtaacacaa cagatcaatt
     3841 aatgacgaat gtatcgacct tgactaaagc accaaaacaa caagaactgg cattattatt
     3901 gacaaccgga gagcaacaaa ctgtatctta tttatcaatg gtattaaatg atatcggtgt
     3961 gaatgccaaa gcaatgactg gctatcaagc gggtattaaa accattggcc atcatttaaa
     4021 aagtaaaatt gctcaaatta atcctcaaac atttgaacaa gcctttcaag aaaacgatat
     4081 tttagtagtt gctggatttc aaggcatcaa tgaacatcag gaattaacaa ctttaggcag
     4141 aggcggttct gatacgaccg ctgtggcact tgctgttagt aatcaaatac cttgtgaaat
     4201 ttataccgac gttgatggtg tgtatgccac tgacccaaga cttttaccaa aagctaaacg
     4261 actagacatc gtctcatatg aagaaatgat ggaaatgagc gctttaggtg ctggtgtact
     4321 tgaaacaaga agtgttgaat tagctaaaaa ctataatatc cctttatatt taggaaaaac
     4381 tttatcgaac gtgaaaggaa catggattat gtcaaatgaa gaaatattag agaaaaaagc
     4441 agttgctggt gtggctttgg ataaacatat gatgcatgta acaattagtt atcccctacc
     4501 tgacaatcag ctacttaccc aactatttac ggaacttgaa gaaggtgctg taaatgttga
     4561 tatgatttca caaatcgtca acttggatgg actacaacta tccttcacga ttaaagatag
     4621 tgattttcat caaatttcta tgattcttga aacattaaag aatcaatatg aagcattagc
     4681 ttataaaatc aatgagcatt atgtcaaaat ttcattaatt ggctcaggca tgcgtgatat
     4741 gtcaggtgtg gcatcaaaag catttttgac attaattgaa aataatatac ctttctacca
     4801 aacaacaact tctgaaataa gtatttcata cgtcattgat gattttaatg ggcaacaagc
     4861 ggtggaaaaa ctatatgacg catttaacat ttaatggtaa aatgattgtt aaaatattct
     4921 aaaaattgga aattattata aaatggagtg acaagttatg acaaaattag cagttgtggg
     4981 ttcaacagga ttagtaggta caaaaatgtt ggagacatta aatcgtaaag atattccttt
     5041 cgatgaatta gtattatttt catcagcacg ttctgcaggg aaagaagttg aatttcaagg
     5101 aaaaacatat acagttcaag aattaactga tgctcgtgca agtgaacatt tcgattatgt
     5161 attaatgagt gctggtggcg gtacaagcga acactttgcc ccactctttg aaaaagctgg
     5221 tgcaatcgtt atagacaatt caagtcaatg gcgtatggca gaagatattg atttaatcgt
     5281 tccggaagtc aatgaaccta cttttacaag aggtatcatt gccaatccaa actgctctac
     5341 gattcagtct gttgtgccat taaaagtatt gcaagatgct tatggtttaa aacgagtggc
     5401 atatacaaca tatcaagctg tatcaggttc agggatgaaa ggtaagaaag atttagctga
     5461 aggtgtaaat ggtaaagcac cagaagcata tccacatcca atttataata atgtgttacc
     5521 gcatattgat gtgtttttag aaaacggata tacaaaagaa gaacaaaaaa tgattgatga
     5581 gacgagaaaa attttaaatg cgccagactt aaaagtaaca gcaacatgtg cacgtgtgcc
     5641 tgttcaagat agtcatagtg ttgaaattga tgtaacgctt gaaaaagaag cgactgcaga
     5701 agatattaaa gcattatttg atcaagatga ccgcgttgtt ttagtagaca atccagagaa
     5761 caatgaatat ccaatggcaa tcaattctac taataaagat gaagtgtttg ttggccgtat
     5821 acgtagagat gattcattag aaaatacttt ccatgtatgg tgtacatcag acaatttatt
     5881 aaaaggtgct gcattaaatg ctgtacaagt attggaacaa gttatgcgtt taaaaggagc
     5941 gaattaaaat gacacattta tttgagggtg ttggcgttgc acttacaacc ccttttacaa
     6001 ataacaaaat taatattgaa gctttgaaaa cacacgttaa ttttttacta gaaaataatg
     6061 cccaagcaat catcgttaat ggaactactg ctgagagccc tactttaaca acagatgaaa
     6121 aagaacgcat tctaaaaaca gttattgatc ttgtagataa acgtgttcct gtcatagcag
     6181 gaactggcac taatgatact gaaaagtcaa tccaagcttc aatccaagct aaagccttag
     6241 gggctgatgc aattatgtta attacgccct actacaacaa aacgaatcaa cgtggtttag
     6301 tcaaacactt tgaagcgatt gcagatgctg tgaaattacc agtcgtgctg tacaatgttc
     6361 cttcaagaac gaacatgaca attgaaccag aaactgtaga aatattaagt caacatcctt
     6421 atatagttgc tttaaaagat gctacgaatg attttgagta tttagaagaa gtgaaaaagc
     6481 gaattgatac aaattcattt gcattatata gtggcaatga tgacaacgtc gttgaatact
     6541 atcaacgtgg cggtcaaggg gttatctctg ttattgccaa tgtcattcct aaagaatttc
     6601 aagcgttata cgatgctcaa caaagtggat tagatattca agatcaattt aaaccaatcg
     6661 gcacactgtt atcagcccta tcagttgata ttaacccaat tcctattaaa gctttaacaa
     6721 gttatttagg atttggaaat tatgaattac gtttaccatt ggttagccta gaagatacag
     6781 atactaaagt gcttcgtgaa gcatatgaca catttaaagc gggtgaaaat gagtgaaaat
     6841 attactaatt ggctatggtg caatgaatca acgcgttgct agattagcag aagaaaaagg
     6901 acatgaaatc gttggggtca ttgaaaatac accgaaagca acaacgccat atcaacaata
     6961 tcaacatatt gcagatgtta aagatgccga tgttgcaata gatttttcaa atccaaatct
     7021 gcttttccct ttattagatg aagagtttca tttgccatta gttgtggcaa caactggcga
     7081 gaaagaaaaa ctacttaata agttagatga attgagtcaa aatatacctg tatttttcag
     7141 cgcgaacatg agttatggcg ttcatgcact gactaaaatt ttagcagctg ctgttcccct
     7201 acttgatgaa ttcgacatcg aattaactga ggcacatcat aataaaaaag tagatgcacc
     7261 aagtggtacg ttagaaaaat tgtatgatgt gatcgtatct ttgaaagaaa atgtaacacc
     7321 tgtgtatgat agacatgaat taaatgaaaa acgccagcca caagatattg gtatacattc
     7381 tattcgtgga ggtacgattg tcggtgaaca tgaagttcta tttgctggca ctgatgaaac
     7441 gattcaaatc acgcatcgtg cacaatcaaa agatattttt gcgaatggtg caatacaagc
     7501 agcagaacgc ttagttaata aaccaaacgg cttttatacg tttgacaact tataaacata
     7561 ttaaaggaga ttgattattt tatggtacaa catttaacag ctgaagaaat tattcaatat
     7621 ataagtgatg ctaaaaagtc tacaccaata aaagtatatt taaatggtaa ttttgaaggc
     7681 atcacatatc cagaaagttt taaagtattt ggttcagaac aatctaaagt aatcttttgt
     7741 gaagcggatg attggaaacc tttttacgaa gcatatggta gtcaattcga agatatagaa
     7801 attgaaatgg atcgtcgcaa ttctgccatt ccattaaaag acttaacaaa tacgaatgca
     7861 cgaattgaac caggtgcgtt tattagagaa caagccatta ttgaagatgg tgctgtcgtt
     7921 atgatgggcg caacaattaa tattggcgca gtcgttggcg aaggtacaat gattgatatg
     7981 aatgctactc tcggtggtcg tgctacaact ggtaaaaatg tacatgtagg ggctggcgca
     8041 gtattagcag gtgtgattga accccctagt gcatcaccgg ttataatcga ggatgatgta
     8101 ttaatcggtg caaatgcagt tattttagaa ggtgtacgtg ttggtaaagg tgctattgtt
     8161 gcagctggcg cgattgtgac acaagatgta ccagctggtg cagttgttgc tggtacacct
     8221 gcaaaagtga ttaagcaagc ttctgaagta caagatacta aaaaagagat tgtagcagca
     8281 ttaagaaaac tgaatgacta gtgacgtcaa cgtataataa tttcaaggtt gagatacgat
     8341 tatgtctcaa ccttattgtt tatcattgaa taaacttatg atcacaaatt tcgaaatacg
     8401 ttacactatt tatatagtaa tttaacacaa aggattgata attatgaatg aattagaatt
     8461 tgttacgaaa catcgccgtc atttacatca acatcctgaa ttaagcttac atgaatttga
     8521 aacaactgct tatattaaag cgtttttaga tagtttaaat attaaatacg attgcccatt
     8581 ggaaactggc gtcattgcat acttagaagg taatggctca catacgatag cgtatagagc
     8641 tgatattgat gccttaccta ttttagagga aaatgatgtg ccttatcgca gtcaatctga
     8701 tcatgtgatg catgcttgtg gacatgatgg tcatacaact gcattaatgc tttttgtaca
     8761 acgttgcaaa gacatgcaag acgcaggtca attaccgcaa aatgtcgttt tcattttcca
     8821 acctgcagaa gaaactggtg gcggtgcaaa tcgattaata aaagccggtg cctttgataa
     8881 gtatccaatt gaagcggtat ttggtattca tgttaaccca tttgctgatg aaggcattgc
     8941 agtgataaga gatgaagaaa ttacggccag cgcaacagag tatcgctttt tcttaacagg
     9001 cctgtcaagt catgttgctg ataaagaaca aggtcattct tgtggtgaag cattacaaca
     9061 tgtattaact caaatatcac aaattcaaca atttcacctt aacggtttga aacgaaatat
     9121 tgttcatatt ggtcatttta aagctggtga agcgattaac actgtaccaa gtaatggtta
     9181 tttagaaggt acaattcgta catatgatat tgatgattta acaatcgtta aaaatcaaat
     9241 gcacaagata gcagaaagtg tcaagcttct gtttaatgta gattgtgaag ttaaatttgc
     9301 agaagattat ccccctacaa tcaatagtcc gaaattacgt actcaaatag aggacgcctt
     9361 aataaaagct gatttaaatg tctatgacaa accaacgcca ttcttatttg gggaagattt
     9421 tagtttttat ggtcaacaac tagctccagc ttactttgtt tttataggaa cacgaaatga
     9481 agataaaggt tttgtaactg gtttgcacac atcacattta aattttgatg aaaaagtgtt
     9541 aataaacgtg gttaattttt acgaaaattt attaaataat tacaaagagg tgtaatacat
     9601 tgacagcaac atggtctgta aataagaaaa tatttttaca aaatgcaatc acagtcaaaa
     9661 acaatcagcc attaatggca gttgttaaaa ataatgcata tcactatgac ctagaatttg
     9721 ctgtaactca gtttatccat gcaggtatag atacatttag cacaacatca ctacgagaaa
     9781 caattcaaat tagacaactt gttccagatg caacaatctt tttaatgaat gcagtttacg
     9841 agtttgattt agtccgtgaa catcaaatac acatgacttt gccgtcattg acatattact
     9901 ataaccataa aaatgattta gcaggtattc atgttcactt agaatttgaa aatttattac
     9961 atcggtctgg atttaaagat ttaaacgaaa ttaaagaagt attgaaagat caccatcata
    10021 atcaaaatgc aaaaatgatt attagtggtt tatggaccca ttttggatat gctgatgaat
    10081 tcgatgtgtc agattataat gttgaacgtt cacaatggat ggaaattgtt gaagcacttt
    10141 tatctgaagg ttatcagttc gacctaatcc atgctcaaaa tagtgcgagt ttttatcggg
    10201 aaggacaaat attactaccc caccatacac atgcacgtgt aggtattgcg ttatacggtt
    10261 caagaccata tagttcactg aatcaacatg atatagttca gtcattaact gtaaaagcac
    10321 atgttattca agtgcgcgaa gtacaagctg gtgattattg cggttatagc tttgcctttg
    10381 aagtgactaa aaacaataca aaattagctg tagttgatat cggttatggc gatggaattt
    10441 taagaacacg tgctaaacat gaagcactta tcaatggtaa acgctacccg atacgtgcat
    10501 taatgatgag ccatatgttt gttgaagtag atggcaatgt acatgcacaa gatgaagtta
    10561 ttctttataa taatgatatc cgcatcgatg aatatacctt taaaggtgtt ggtgcaaatt
    10621 ctgaacaatt aagtgctatg aatcatgatt ctttaaaaaa ggagtacatt tcaaatgact
    10681 gttaaatata atcaaaatgg cgaattaaca atggatggta ttagtttaaa aacgattgcg
    10741 caaagctttg gtacacctac cattgtttat gatgaactac aaattagaga acagatgcgc
    10801 cgttaccatc gcgcatttaa agatagtgga ttaaaataca atatttcata cgcctcaaag
    10861 gcatttactt gcattcaaat ggtcaaactt gtagctgagg aagatttaca gttagatgtt
    10921 gtttctgaag gtgaattata tacagcttta gaagcaggtt ttgaaccgag tcgcatccat
    10981 ttccatggta acaataaaac gaaacatgaa attaggtatg ctttagaaaa taatatcggt
    11041 tattttgtta tagattcatt agaagaaatt gaattaatag accgctatgc taatgatacg
    11101 gtgcaagttg tattacgagt taatccaggt gttgaagcac atacacacga atttattcaa
    11161 actgggcaag aagatagtaa gtttggatta tcaattcaat atggcttagc taaaaaagca
    11221 attgacaaag tccaacaatc taaacactta aaattaaaag gtgtacattg tcatattggt
    11281 tcacagattg aaggtacaga agcatttatt gaaactgcta aaattgtttt acgttggctt
    11341 aaagagcaag gcattcaagt tgaattatta aaccttggtg gtggctttgg tattaaatat
    11401 gttgaaggtg acgaaagttt ccctatcgaa agtggtatta aagatattac agacgcaata
    11461 aaatccgaaa ttaaagttct aggtatagat gcaccagaaa taggtattga accgggacga
    11521 tcaattgtag gtgaagctgg cgttacttta tatgaagttg gaaccattaa agaaattcca
    11581 gagattaata aatatgtttc aatcgatggc ggtatgagtg atcatatcag aactgcactt
    11641 tatgacgcaa agtatcaagc attgcttgtt aatagaaatg aagaagcaga tgacagtgta
    11701 actatagctg gaaaattatg tgagtctggt gatatcatta ttaaagacgc taaattacct
    11761 tcatcagtca aacgtggaga ctatcttgct atattatcaa ctggtgcata tcattactct
    11821 atggcatcca attacaatca aatgcaaaag ccttctgtgt ttttcttaaa agatggcaaa
    11881 gcacgtgaag ttataaagcg acaatcgtta agacaactca ttattaatga tacaaaataa
    11941 aaataaacaa agtaatcccc gaagcacaga aaattatact gtgactcggg gaatttttat
    12001 gcattataga tgactgtaga aaacatgctt ggacatatac tctaaaaaag gaagaacagc
    12061 acatttttag ttagaaagta tttatttctt tttgtcatta atcctttata tttagttagc
    12121 cctattcaac taatttaaag catcatatac ataccacact ttaaacaagc atcatgaaat
    12181 tagattttat gctttacttg ctttttcaaa taatataaaa cga
//
LOCUS       DAMZLE010000075        12115 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMZLE010000075 DAMZLE010000000
VERSION     DAMZLE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.075"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            55..183
                     /locus_tag="POB92_001749"
     CDS             55..183
                     /locus_tag="POB92_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579236.1"
                     /translation="MIKTMSIENMCTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(328..2256)
                     /locus_tag="POB92_001750"
     CDS             complement(328..2256)
                     /locus_tag="POB92_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI6579237.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIITGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2509..3144
                     /locus_tag="POB92_001751"
     CDS             2509..3144
                     /locus_tag="POB92_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDI6579238.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3448..4527
                     /locus_tag="POB92_001752"
     CDS             3448..4527
                     /locus_tag="POB92_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDI6579239.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILKIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            4587..4811
                     /locus_tag="POB92_001753"
     CDS             4587..4811
                     /locus_tag="POB92_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDI6579240.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            5019..6269
                     /locus_tag="POB92_001754"
     CDS             5019..6269
                     /locus_tag="POB92_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDI6579241.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDKIAMLAFTNTVISASAGSIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAALTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6452..7402
                     /locus_tag="POB92_001755"
     CDS             6452..7402
                     /locus_tag="POB92_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6579242.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANALENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7551..9035
                     /locus_tag="POB92_001756"
     CDS             7551..9035
                     /locus_tag="POB92_001756"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI6579243.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYSEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            9032..9991
                     /locus_tag="POB92_001757"
     CDS             9032..9991
                     /locus_tag="POB92_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDI6579244.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(10179..10288)
                     /locus_tag="POB92_001758"
                     /pseudo
     CDS             complement(10179..10288)
                     /locus_tag="POB92_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10360..11076)
                     /locus_tag="POB92_001759"
     CDS             complement(10360..11076)
                     /locus_tag="POB92_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6579245.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(11095..>12115)
                     /locus_tag="POB92_001760"
     CDS             complement(11095..>12115)
                     /locus_tag="POB92_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDI6579246.1"
                     /translation="ANFVYIIIFSYIITISHSVFIVLPIFLVVYVSISYALAYILNRI
                     LKRINGTYLSLNKKFLTVITIVIVITFSLLFAYSQIDASDASTIKQYSLLFLGIIILL
                     SILIFIYSQFTLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEY
                     IREDDMTGLRDYFNKNIVPMKDNLQMNALKLNGIENLKVREIKGLLTAKILRAQEMNI
                     PISIEIPDEVTRINLNMIDLNRSIGIILDNAIEASSEIDDPIIRVAFIESENSVTFIV
                     MNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKV
                     EIINN"
BASE COUNT         4112 a         1719 c         2142 g         4142 t
ORIGIN      
        1 aatatacttc aatattatta ttatttcata aagaaaatgt cttaacaatg aattttgata
       61 aaaacaatgt caatagagaa catgtgtact tatcaacaaa gagtgaataa cttgtggata
      121 aaaggtgttg ataacgtcat ttattgtgga tatcaggtgg gaaaacagaa aataaatgat
      181 tgattagtgt taatttactt gtggataact aggataactc tgtggatagc tataatatca
      241 gcgtttttat tgtgaataaa aaaatttaaa atttgtaaaa aaataactct aactatagcg
      301 attttacagt tagagttatg tatattttta tagtttttgt tgtaattctt cccatttaga
      361 catagcgtgt tctaattttt gttcgctatc ttgtttttgg atagctaatt cgtttgcttt
      421 ttgtggattg ttataaactt ctggttgggt taattgctcg tctatttgta agatagtagt
      481 ctctaaagtt tctatttcat tttcacactc ggatatttgt cgttcaagtt tgcgttgttc
      541 acgtctttgt tctttttggt tatgatacga cgattgttcc atagactttt catgtgcttg
      601 tacgttaact gactgttctt cttggtgcgc tttcaacgct tccgcttcct caactttttc
      661 aatgtaatat tgataatctc ctaaatacat ctttccgcca tcaattgtta aatcaaatac
      721 cttgtttgct aattggttaa taaagtaacg atcatgggaa acaaatagta ttgttccttc
      781 aaaatgttgg agtgcttgct ctaacatttc ttttgaatct atatcgaggt ggttggtagg
      841 ttcatctaaa ataagtacat tgtcacgttg caacataagt agtgctagtt gtaaacgtgc
      901 tttttcacca ccagataaat cattaattat ctttttaaca tcgtcttgta caaataagaa
      961 acgaccaaga actgcacgaa tatctttttc attcattaac ggatattgat cccacacata
     1021 atctaaaatc gttttactag atttaaattc tgcttgtttt tgatcataat aaccaatttg
     1081 taaatttgcg ccgaaagtaa tatcgccatt aagcgctttt tgttgattag caatagtttt
     1141 aattaaggtc gattttccaa taccatttgg cccaatgatt gctatatgat cgcctttaga
     1201 gacctctata ctgataggtt tggtaattgc agtttgataa ccgatttcta aattttttac
     1261 atgcatgacg tcattacctg tattccggtc aaagccaaat tgaatatttg cacttttggc
     1321 atctaacatt ggtttatcaa tgcgttccat tttttctaaa atcttacgtc tactttttgc
     1381 cattccactt gttgaagcac gggtaatatt tttctcaaca aaagtttcta atcgttttat
     1441 ttctgcttgt tgactttcat attcttgcat tcgtttttga taatataaat cccgttgctg
     1501 tataaattcc tcgtaattac caacatagcg tttgacatca cctaaagcca catcataaat
     1561 ttgagtaact attttatcta aaaagtaacg atcatggctg atgatgacga ttgcaccttt
     1621 aaaataacgt agataatctt caagccactt tgtcgtttct aaatctaagt ggttagtagg
     1681 ttcatctaaa agtaataaat caggttcgtt taatagcatt tgagctaaag ataaacgtgt
     1741 tttttggcca ccgctaaaat cattgatagg tttattgaaa tcttcttcac taaaatttaa
     1801 cccgtgaagt actgttttaa ttttactttc atattgatac ccttctaatt gttcaaattg
     1861 attcgataaa gattcataac gggacatatg tgttttatat gtatcactat cgtaatcatt
     1921 tgcatgtttt gataaccaat ctgtttcttc tttgattaag ctttccattc gtttaatatg
     1981 ttcaaatggt tttgacattt cttcaaaaac tgttgcatta gaatttaaag tcatttgttg
     2041 agttaaataa cctagtttta agtttttaat ttttgacaca tttccagaat catagttttc
     2101 tacgccagtt ataattttca tcaatgttga tttaccggcg ccatttcttc ctacgatacc
     2161 aattcgttca ccagttttta cttcaaaatc aacatcagta aatatatctt caccatcgaa
     2221 cgattttgat atatgattga gttgtaaaag tatcattcga tcttcacctt tcgtctttct
     2281 caaattttat taacttatga aaaaagtcgt aattattaca ttaataacca ttttacagta
     2341 taaaacgccg tcttgaaact aagatattta tttttaaaat tcaataatgt ataatattta
     2401 ggtattcatt gtaatcaatt atttgttttt taataatcta acaattacgc tctaactcgt
     2461 ttgaatacat agatataatt cattcaaaca cgaaggagga aatagcgaat gagtgaccaa
     2521 gttaaaattc ctcgagcaac tttaaaacgt ttgccgttat attatagatt tgtcagttca
     2581 ttaaaatcta aaggtataga tcgtgtaaat tcaaaagcga ttagcgatgc gttacaaatt
     2641 gactcggcaa caattcgtcg tgacttttca tattttggcg aattaggtaa aaaagggtac
     2701 ggatataata tagatagttt attggatttc tttaaatctg aactaagcga gagtgacatg
     2761 atcaaaatcg caattgtcgg agttgggaac ctagggaaag ctttgctcac atataacttt
     2821 tcaatacatg acgatatgac gattacagaa gcgtttgacg taaaagaaga tgttattggc
     2881 cagaaaatag ggaacgttat tgttaaagat aacgatgaat taataacaac attgaagaag
     2941 gaagaaatag atgttgtgat tctaactaca ccagaaagag ttgcacagaa agttgcagat
     3001 gaactcgtcc aagctggtgt gaaaggtatt ttaaacttca ctcctggtag aattaatacg
     3061 ccttcagatg tgcaagtaca tcaaattgac ttaggtatag aattacagtc attattattc
     3121 tttatgaaaa attacagtga ataaatgtga tgttagcttt gaatgattaa ccttgttata
     3181 tctttaatta attatagaac agtctgggac taagtatgtc tcaggctttt tctgttatgt
     3241 ttgaaaatga aaggaaaaac tgttgtgctg ataaatatag tataggttaa taaattaaca
     3301 cgtagagata taacgatgaa tcgtaattaa tcgccttcct tatatgaata taatataaat
     3361 atcaaaaata ttgtgtataa ggttgaaaac aagaaaatca tcacctataa tacatactaa
     3421 acttatcgga aatgagaggg taggattatg tggatgattt ttagtggctt aattgtcggg
     3481 ggactcttag gttttgtcat gcaaagaaca agattttgtt taacaggtgg ctttcgagat
     3541 atgtatgtgc aaaagaataa taagatgttc tatgcattat taatcgctat tactattcaa
     3601 agtataggat tattgatttt gacggcaaca gatattttaa aaattcctgc acatagtttt
     3661 ccaatattgg gaacaattat aggttctttt atttttggaa ttggaatagt attggctgga
     3721 gggtgtgcaa caggtacttg gtatcgcgct ggtgaagggc taattggtag ttggattgca
     3781 ttagtattat atgctgttac tgcagcaatc actaaaacag ggattttaaa gccagtaatg
     3841 gataaaatta atcaaccaac gaatgtaaat agtgatatgt ctcaaacaac tggcattccg
     3901 ttttggggat tagtcgttat attaactata atcaccattt ttctagttgt aagaacactc
     3961 aataataaga aagttcgagt tgctgttcca aaactaaagc aacgttatac aggtattaga
     4021 tattaccttt tcgaaaaacg ataccatcca tttattgcag caattgtaat tggacttatc
     4081 gcactcttag cttggccaat gagtgcatca actggaagaa atgacggttt aggtataaca
     4141 acgccatcag caaatttagt acactttttg attacaggtg aaactaaatt tattgattgg
     4201 ggtgtctttt tagttctagg aattttcatt ggttcatata ttgcagctag aggatcaaga
     4261 gaatttaaat ggcgattgcc agataagatt acaatacgaa acagtgctat tggtggcata
     4321 tgtatgggat ttggtgcgtc agttgctggt ggttgttcta tcggtaacgg tttggttgaa
     4381 acagcaacga tgacttggca aggatggatt gcgctagcat caatgatagt tggtgtatgg
     4441 acaatgagtc attttatctt tgttcgtcca atgaaaaaag tacaacaaca atctgcaaag
     4501 gttaaacagc aaacgcaaat agtatagaag attattatgc aaatgatgtt gatcaaataa
     4561 aagtgattgg aaaaggagaa ataattatga tacacgaatt aggtacagta ggaatggtat
     4621 gtccatttcc gttaattgaa gcgcaaaaga aaatggcaac attgcaatct ggagatgaat
     4681 taaaaattga ttttgattgc acgcaagcga cggaagctat tccaaattgg gcagcagaaa
     4741 atggatatcc tgtaacaaac tatgaacaaa ttgataatgc ttcatggaca attacaattc
     4801 aaaaagttta acgttatcat tttaacaata aaatagatgt tagattctat ggctacttcc
     4861 gctaatttaa aagtgagtaa gtagtctttt ttgttttagt tcatgaaatc acttttatat
     4921 agtgtggcac attttatccc aaaagatgta ataaaactta acgcattttt gctttttata
     4981 aattgtcaga ttattatgaa aacaagggag tggtaagtat gaatcttaac gatacgatat
     5041 ttatgttttt gtgtacatta ttagtttggc taatgacacc aggattaagt ttattttatg
     5101 gtgggttagt tcaatctaaa aatgcgctta atactgtcat gcaaagtatg gcagcaattg
     5161 tgcttgttac atttgtatgg ataacagttg gttttacaat tagttttggg aatgggaatt
     5221 tatggttcgg aaattgggaa tatacttttc ttaatcatgt aggttttgcg actcaagaag
     5281 atattagccc acatattcct ttcgctttgt ttatgttatt tcaaatgatg ttttgtacga
     5341 ttgcaatttc tattttatct ggttcaatcg ctgagaaaat gaagtttatt ccttatttat
     5401 tattcgtagt aatatggact gctcttgtat acagtccagt agcacattgg gtttggggcg
     5461 gcggttggat taacaaactc ggtgtattag atttcgctgg aggtacggtt gttcatatta
     5521 catcaggtgt ttctggttta gtattagcta ttatgattgg aaaaggaaac aaacattctg
     5581 aatcaacacc acataatctt atcattacgt tgattggcgg tatattcgtg tggattggtt
     5641 ggtatggatt taatgtaggt agtgctttta catttgataa aattgcgatg cttgcattta
     5701 caaatactgt catttcagcc agtgcaggtt ctataggttg gttaatttta gaatatattt
     5761 ttaaaaagac gacaagttta cttggacttt tactcggtgc attagcagga ttagttgtca
     5821 ttactcctgc agcaggatat gtaacatatc ttagtgcaac aataatggct ttaataggag
     5881 gtatctgttg ttatattgtc attaattaca tcaaggtaaa actaaaatat catgatgcat
     5941 tagatgcatt tggtatccac ggtgttggtg gtattattgg tgctgcttta acagcagttt
     6001 tccaaagtaa aaaagcaaat cctgacattg aaaatggttt tatttatact ggtgacatac
     6061 atattatact tgtacaaata ttatgtgtaa cagcagttgt aatttttagt atcgtcatga
     6121 cgtttattat tgcgaaagta attaaattaa ttacaccatt atctgttacg gaacaagaaa
     6181 cgaatatagg attagacaag attgttcacg gtgaacatgc ttactttgaa ggtgagctaa
     6241 atagattcaa taaacatatt cgatattaga atatatttac atagaatatt cattgtcctg
     6301 acatttaact aaaggttgat gttgggacat tttgttatac aaaagtttta ttttgaaatc
     6361 tttttatgaa agaagcagaa atattattta aagcggttac acatatgcta aaataaggct
     6421 aagtgtcaca aataatgata ggtgaataag tatgaaaaat atatctgata ttgccaaatt
     6481 ggcaggcgtt tcaaaaagta cagtatctag atttttaaat aatggatctg tcagtaaaaa
     6541 aacaagtgaa aaattaacaa gaattatagc agaacatgac tatcaaccga atcaatttgc
     6601 tcaaagttta agagcgagac aaacacacct tattggtgcc attataccta gaatgaattc
     6661 atatgcagta gacgaaacaa tcaaaggatt ggcaaaacaa tgccaaaaat atgaatcaca
     6721 attaatttta aattatacag gtttaaatat cgaagcagaa atacaagcgc ttgaaacatt
     6781 agcacgcagt aaagtagatg gtattgtttt aatggctaca gatataacag agagacatat
     6841 tgaagtcatt aataaaatga atgtaccaat cgttattgtt ggtcaacaac atgaacaact
     6901 tcatagtatt gtgcatgatg attataaagc aggtcaaatt ataggcgaat ggattggtca
     6961 acagggatat caacaagttg aagtgtttag cgtaagtgaa aaagatattg cagttggtat
     7021 acatagaaaa cgtggtttac ttgaccagtt agctaaatac caaattaaac ctaatataca
     7081 tgaaacaaat tttacttatg tggaagcaca aaaagatgtt gcaaatgctt tggaaaatgt
     7141 ggagcaagta gatgcggttg ttggagcaac tgatacgatt gcattagctg cctataaata
     7201 ttattctgat aaaaaagatg ttatgaaacc acatcaaata tatggttttg gtggtgaccc
     7261 aatgacacaa ttagtgtctc catcgataaa aacaattcat tataattatt ttgaagctgg
     7321 ccaatgcgcg atggaagaga tacaacagat gcttaaaaag caagatatgc catatagcgt
     7381 cacagtagat gttaatattt agacgctgta ttttttaaaa taaatgtgga accgatacca
     7441 tataactata aatggatagg ttaaaagtta aagaacgtag gtaaaatttg ctataataga
     7501 atataaattg ttaacagcat aaattataaa aggaggactg ggtaaatatt atgaccgaat
     7561 ggactagaga agaacgttat caacgaatcg aggacgttga tactgagtat tttaaaacat
     7621 taaaacaaca agttgatcaa tcaaaatttc gtcaacaatt tcatatacaa ccagaaacag
     7681 gcttattaaa tgaccccaac ggactaattt tttataaagg gaagtattat gtttcacatc
     7741 aatggttccc attaggcgca gtacatggct taaagtattg gtataactac acgagtgatg
     7801 acttaataaa ctttaaagct gaagggccaa ttttaaatcc agatactaaa tatgacagcc
     7861 atggtgtata tagcggtagc gcttttgaat ataacgggca tttatattat atgtacacag
     7921 gaaatcatcg agataatcat tggcaacgac atgcgagtca gatgatcgca cgattgaaag
     7981 aagacggttc agttgaaaag tttccaaagc cagtaattag ccaacaacca gaaggatata
     8041 cgagtcattt tagagatcct aaagttttta aatatagtga gaaatattat gcgatcattg
     8101 gcgcacaaaa taatgatcag caaggtcgat tattacttta taatactgaa gatataatta
     8161 attggcatta tttaggtgaa ataaatacag agttggatga ttttggatat atgtgggaat
     8221 gcccagatta ctttaatgta gataatcaag atgtcatact tatttgtcca caaggtattg
     8281 aaccaaaagg cgatcagttc aaaaatattt atcaaagtgg ttatatactt ggaaagtttg
     8341 atattgaaaa gttaacatat gaacatgaaa attttgtcga gcttgataat ggctttgatt
     8401 tctatgcacc tcaaacattt ttagatgaaa aaggccgacg agtactaatt ggatggatgg
     8461 ggttaccgga aatcgaatat cctactgata atgaaggatg ggcccattgc ttaacaattc
     8521 ctcgtgtatt aaatgtagaa aatggtcaac ttaagcagcg tccatatcca gcgttggaaa
     8581 aattacgtca caataaagag acagcattag gctacgcaaa taaatttact cgaaaattac
     8641 atccgtatga aggtaaacag tatgaattaa tcatagatat tttggataat gatgctaccg
     8701 aagtgtactt tgaattacgt acatctaaga cttcttcaac attaatcgct tataacaagc
     8761 gtgaaaataa aataacatta gatcgcagcg acagtggttt attgccgaca aatgttgaag
     8821 gtacgacgcg tagtacgata ttagacacgc cattaaagca attacaaatt tttgttgata
     8881 catctagtat agaaattttc tgtaatgatg gggaacgtgt tttgacatct cgaattttcc
     8941 cgacagaaga cgcactaggt attaaaacat caacagaatc agggcaagta tatttacaat
     9001 ttactaaata tgatttaaag gatgaacata aatgagacgt ctattttcaa taggagaagc
     9061 gttaattgat tttattccaa atattacaaa tgcgaattta aaagatgttc aaacatttac
     9121 gaagcaaatt ggtggcgcgc catgcaatgt agcttgtaca gttcaaaagt taggacaaca
     9181 agcgtatatg attacacaat taggtaatga tgcatttgga gatagcatta ttgaaacgat
     9241 atcaagtatt ggtgtagatg tatcaaaagt gtatcgcacg aatgaagcaa acactgcgtt
     9301 agcttttgtg agtttgactg aagcgggcga gcgagatttt tctttttatc gtaagccctc
     9361 tgctgatatg ttatttgaac caagttttgt gaatgatatt gatgtgaatg aaaatgatgt
     9421 agtacatttt tgttctgttg atttggtcga tagtccgatg agagatgcac attatcaatt
     9481 gattaccaaa acattaaatg caaatggtac tgtcgtattt gatcctaatg taagattgcc
     9541 attatgggat aacgctgaag atttaagaca aactattcat acctttttgc cattggcaca
     9601 tattgtaaaa gtttcagatg aagaattaga atttattaca ggtattcatg atgaaaatga
     9661 agccattcaa tcattattta caggtaatgt cactgtagtc atttatacga agggagcaga
     9721 tggtgctgcc gtatatttga agaatggtat taatcattat catagtggct ataaggtaaa
     9781 acctgttgat acaactgggg caggggatgc ttttattggt gcagttatta gcaggatttt
     9841 agcaaccgat gtgttaaatt taactcagtt atttgaaaat gaaggtgagg aaattttagc
     9901 gtttagcaat cgcgttgctg caatagtgac aacgaagtat ggtgctatta atagtttgcc
     9961 aacattacaa gaggttgaac aagcatttta aataattcat ttataaaaca gtgtaggaaa
    10021 caagttgaat cttgtgctac actgtttttt aaaacaaaat aagattcacg gggtaggaaa
    10081 ttgtagcatt ttatttatgg aaactgtcaa tttcaaattt agtatatttt gttaaaaatt
    10141 gcgccatagg attgtagagt gaaatagaaa ttgataaatt atgtagtaat aggaataggt
    10201 atacaaagaa acgttacatc attttttaat tcacgcgtca tatttaattt tgttattaaa
    10261 caattaaaaa gccagctata cagtgcattt gctagttatc ttgttaaaat ccaacaagat
    10321 ttacaattga atacgccgtt aactgacttt attatcttat tatatttttt taacgtttct
    10381 caccgatgca tagcagtgtt ctttattttt aaaatagaca attcgctctt tcgaatctat
    10441 agattcaata ttatggcgat tgacgacaaa gctattatga catctaaaga aacgatcatc
    10501 taattgactc agttctttta aattaccata aaattcaatt tgacggttat ctaaatgggc
    10561 aatgagtctg tgagattttg ttgatgattc aaaaaacata atatcatcat attgaacata
    10621 cactgaatta ctgccacgtt ttaattcaat cgtttcaacg ctattatctt tagacaacaa
    10681 ttgtaagcgt gtatgtgcag tttctaaaca atctataatt cgagttctta attctgctgg
    10741 atcatcttta aaaataaaat ccatcgctgc aactttgtag acaaatgtta aataggtaag
    10801 ttcactgtga ctcgtaacga aaataatgtt accaactggg tcatgcttac gaatttcact
    10861 gcctaatttg ataccattaa tatcagttga aagttgaata tctaaaaagt aacagcctat
    10921 gtcattcata tttttagctt gctcaagcac ctcataagga ttatcagttg cgagggcaat
    10981 ttccataggc ttttcttcta tcattatata atttttaata atggtaacca tgttttctct
    11041 ttgttttgga tcgtcttcgc aaatgaaaat tttcatacat tcacatcctt atggctagtt
    11101 gttaataatt tcaacttttt gaataaagaa accattttcg ataattgtat ctaataagac
    11161 attgtctgca ttatcagcaa tttcttttaa agttgataga cctaaaccac gaccttcacc
    11221 tttagtagaa aaactttctt ggaacaattc atgaatacgt ggtatatcat ctgcacattt
    11281 attcataaca ataaacgtta ctgaattttc gctttcaata aatgcaactc gaatgatagg
    11341 atcatcaatt tcacttgatg cctctattgc gttatcaaga ataataccaa tactgcgatt
    11401 taaatcgatc atattcaaat taatgcgagt tacttcatct ggtatttcta tactaatcgg
    11461 tatattcatt tcttgtgcac gtaaaatttt agctgttaga agacctttaa tctcacgtac
    11521 tttaagattc tcaataccat ttaacttaag agcattcatt tgtaaattat ctttcatagg
    11581 tacaatattt ttattgaaat agtctcgaag accggtcatg tcatcttcac gaatgtattc
    11641 tgaaagagtc gtcaagatat tgacataatc atgacggaac ttgcgcattt cattattaat
    11701 tgcttcaatc tttaatgtgt attcataata ggtttcaatt tcttcttgat tacgtttata
    11761 tttcatttct ttaagagtaa attgagaata aataaaaatt aagatactca gtaatataat
    11821 aattcccaag aataaaagcg aatactgttt gattgttgat gcatcactcg catcaatttg
    11881 cgaatatgca aataacaaag agaatgttat tactataaca atagttataa cagtaaggaa
    11941 ttttttatta agagaaaggt atgtaccatt tatccttttt aatattctat ttaaaatgta
    12001 ggcaagtgca taagaaattg atacatacac taccaaaaag attggtagaa ctataaacac
    12061 gctgtgtgaa attgttataa tgtatgaaaa aataatgata taaacgaaat ttgca
//
LOCUS       DAMZLE010000076        12035 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMZLE010000076 DAMZLE010000000
VERSION     DAMZLE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.076"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            69..830
                     /gene="sufC"
                     /locus_tag="POB92_001761"
     CDS             69..830
                     /gene="sufC"
                     /locus_tag="POB92_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI6579247.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            928..2235
                     /gene="sufD"
                     /locus_tag="POB92_001762"
     CDS             928..2235
                     /gene="sufD"
                     /locus_tag="POB92_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI6579248.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVIGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2341..3591
                     /locus_tag="POB92_001763"
     CDS             2341..3591
                     /locus_tag="POB92_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6579249.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            3581..4045
                     /locus_tag="POB92_001764"
     CDS             3581..4045
                     /locus_tag="POB92_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDI6579250.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4196..5593
                     /gene="sufB"
                     /locus_tag="POB92_001765"
     CDS             4196..5593
                     /gene="sufB"
                     /locus_tag="POB92_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI6579251.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(5661..6711)
                     /locus_tag="POB92_001766"
                     /pseudo
     CDS             complement(5661..6711)
                     /locus_tag="POB92_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(6774..7289)
                     /locus_tag="POB92_001767"
     CDS             complement(6774..7289)
                     /locus_tag="POB92_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579252.1"
                     /translation="MKRLLYLILASALVLGACGSNDGDKKEESKKTETKKENKDKKKE
                     TKDKAEAKKENANQNDNNNQVNKDNNTNVNDHQQTNNAPKQNQTQNNPTSNKNNNAPV
                     KDEFSSDTSYNAYQDAKRATEENKRQNGGHTAGIGGSWAVQDGQDYNSWKKAQNDFDN
                     FKRQNSEVIQQ"
     gene            complement(7293..7793)
                     /locus_tag="POB92_001768"
     CDS             complement(7293..7793)
                     /locus_tag="POB92_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579253.1"
                     /translation="MAGDKLTFKEILTETKMFSKLSNRKIDMYKKMTTDEKRKILNDF
                     KEGKELDIQLYKSENFKNTNEEYESKSAKSLNGQGIKEATDVTTYAYQKQNINPTLLK
                     VYNGLGTFTTNVDKQAKFVFYDTQLKQNFVSIAQRDELIKQNNRIIEQNNEVIDLLKQ
                     IANKGV"
     gene            complement(7844..8482)
                     /locus_tag="POB92_001769"
     CDS             complement(7844..8482)
                     /locus_tag="POB92_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDI6579254.1"
                     /translation="MAFKNSIKEIRLNNRLSKVEMARKLDVSEGTIRMWESGRTEPRM
                     GMVEKISSLFNVSKGYLLGEIEEIVLPEFDSEIEVPYFGKVSAGNFEEVAIDNEKLKV
                     PPFAFNGRKPSECIALKINGDSMNKILANGSYIIVHDYRKSCDHKLNSNDILVLRLGG
                     EYTVKRVRRTETKLHLDPVSYSDEFKTNSYDLDSIDEIEVIGKVIYNYRIFD"
     gene            8672..8926
                     /locus_tag="POB92_001770"
     CDS             8672..8926
                     /locus_tag="POB92_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015997083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6579255.1"
                     /translation="MNYIKHSLKLDEWRKRKGYTQSSFAEKLGISPSTYNIWENNPEM
                     IKPRDAFRIAKTLDISIDEIIFLKDESYFKYVLVEEKQTI"
     gene            8942..9259
                     /locus_tag="POB92_001771"
     CDS             8942..9259
                     /locus_tag="POB92_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579256.1"
                     /translation="MQQERDRLLKLTRLQLKYSNLAGQIEAYDKTLKEIRYTRDLYNK
                     YLSMNNYDAFAGLEMVEDEITEKLRSVIKELQKIEKEMNKLNGVESDNKVTDLTEWRK
                     VNQ"
     gene            9414..10205
                     /locus_tag="POB92_001772"
     CDS             9414..10205
                     /locus_tag="POB92_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6579257.1"
                     /translation="MQSLQTFNFKELPVRTVEIENEPYFVGKDIAEILGYARSDNAIR
                     NHVDSEDKLTHQFSASGQNRNMIIINESGLYSLIFDASKQSKNEKIRETARKFKRWVT
                     SDVLPAIRKHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKAD
                     YVDEILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTD
                     SDTITIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANLGGA"
     gene            10206..10430
                     /locus_tag="POB92_001773"
     CDS             10206..10430
                     /locus_tag="POB92_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579258.1"
                     /translation="MTPEQKEKLNNIVLTLYAVKENKSQTYTHKDTLTVTYAGEIEHT
                     YEVDREKHLESMIEWAIDQIEQHFDLDEEE"
     gene            10470..10919
                     /locus_tag="POB92_001774"
     CDS             10470..10919
                     /locus_tag="POB92_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579259.1"
                     /translation="MNALYKTTLLITMAVVTWKVWKIERNTRKPVINRNDFSKESTAE
                     TIERHSDPDSGIKLLKAFSDFTKQAEKQKPTLGEVYRRNKPELPTVTLDENGLFINDF
                     RVPYVLEEGVNVKKSMNNLYKVSLDFFAKSIIADNYEADNPENQQLF"
     gene            10933..11154
                     /locus_tag="POB92_001775"
     CDS             10933..11154
                     /locus_tag="POB92_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579260.1"
                     /translation="MIKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHGLSDERIMEALKKENE"
     gene            11147..11314
                     /locus_tag="POB92_001776"
     CDS             11147..11314
                     /locus_tag="POB92_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDI6579261.1"
                     /translation="MSKTYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYGVDD"
     gene            11315..11635
                     /locus_tag="POB92_001777"
     CDS             11315..11635
                     /locus_tag="POB92_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579262.1"
                     /translation="MTWFEEYVKPSVEWERKAEQAVLSDDEVKTITEYRKKYNNPHIY
                     MSAQNRNYLVEYLDRHTGDIVLHNSKLKKSSRRRVHQYLMVGQIVVPGEPKGTIYEAS
                     LIIR"
     gene            11729..11989
                     /locus_tag="POB92_001778"
     CDS             11729..11989
                     /locus_tag="POB92_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6579263.1"
                     /translation="MYYEIGDVCQKVINVDGFDFKLAVKKKDHSILVNILDLEDKFID
                     GINITNENDLYTALDILNQSIYEWIEENADDYDRLINLVMKW"
BASE COUNT         4190 a         1860 c         2212 g         3773 t
ORIGIN      
        1 tttgactaca aattaaaatt attataaact agttaagaaa actttatatt ttacggaggg
       61 aatataaaat ggcatcaaca ttagaaatca aagacctaca tgtgtctatt gaggataaag
      121 aaatcttaaa aggtgttaac ttgacaatta acactgatga aatacatgcg attatgggac
      181 caaacgggac aggtaaatca actttatcat ctgcaattat gggacaccca agctatgaag
      241 taactaaagg agaagtactt ttagacggtg taaatatttt agaattagaa gttgatgaaa
      301 gagcaaaagc aggattattc ttggcaatgc aatatccatc agaaattaca ggtgttacaa
      361 atgctgattt catgcgttca gcaatcaatg cgaaacgtga agaaggacaa gaaatcaact
      421 taatgcaatt tattaagaaa ttagataaaa acatggattt tctagacata gataaagaca
      481 tggcacaacg ttatttaaat gaaggtttct caggtggaga gaagaaacgt aacgaaatct
      541 tacaattaat gatgttagaa cctaagtttg caatcttaga tgaaatcgat tcagggttag
      601 acatcgatgc attaaaagtt gtatctaaag gtattaacca aatgcgtggg gaaaactttg
      661 gtgcattaat gattacacac tatcaacgat tattaaatta cattactcct gataaagtac
      721 atgtaatgta tgctggcaaa gtcgttaaat ctggtggtcc agaattagca aaacgtcttg
      781 aagaagaagg atatgaatgg gttaaagaag agttcggttc agctgaataa tcttattaat
      841 acagtatcca tgagatgttc atctatatat gatgaaaatg aacatttata cgaaatagta
      901 aatttcatca agtaggagga aaaagttatg acaactgata ttttgaacat ttctgaagaa
      961 caacttgttg attattctaa agcccacaat gaaccttctt ggatgacaga attacgtaaa
     1021 aaagctttga aattaacaga aactttagaa atgccaaaac ctgataaaac aaaattaaga
     1081 aaatgggatt ttgattcttt taaacaacac gatgtaaaag gtgatgttta tcaatcttta
     1141 tcacaattac ctgagtcagt aagagaaatt attgacgtag atcattctaa aaacttagta
     1201 attcaacata ataatacgat tgcgtacaca caagttgatg ataatgcatc gaaagatggc
     1261 gttatcgttg aaggtttagc agacgctctt atgaaccata gtgatttagt acaaaagtac
     1321 tttatgaaag atgcagtaac agtagatgaa catcgtatca cagcgctaca cacggcatta
     1381 gttaatggtg gcgtatttgt ttatgttcct aaaaatgtag ttgtagaaca tccagtacaa
     1441 tacgttgtgt tgcacgacga cgaaaatgca agcttttata accatgttat catcgttact
     1501 gaagaaagcg ccgaagtcac atatgttgaa aattacttat caaatgcatc tggtgaagga
     1561 aatcaattaa atattatttc tgaagtgatt gctggtgcaa attcaaatat cacatatggc
     1621 tcagtggact atatggataa aggctttaca ggtcatatca ttcgacgtgg tattactgaa
     1681 gcggatgcct caattaattg ggcactaggt ttaatgaatg agggtagcca aattattgat
     1741 aatacaacaa atttatttgg tgatcgttca acaagttcac ttaaatcagt agttataggt
     1801 acaggcgaac aaaaaattaa tctaacatct aaaatcgtac aatatggtaa agaaacagat
     1861 ggttatatcc ttaaacatgg tgttatgaaa gaacatgcat cgtctgtatt taatggtatc
     1921 ggctacatta agcatggtgg aactaaatca attgctaatc aggaatcacg tgtattaatg
     1981 ttatctgaac atgctcgtgg tgacgcgaat cctattttat taattgatga agatgatgta
     2041 caagctggtc atgctgcatc agtaggtcgt gttgatccag atcaacttta ctatttaatg
     2101 agtcgtggta tttctcaaag agaagcggaa cgtcttgtta tacatggttt cttagatcca
     2161 gtagtacgtg aattacctat cgaagacgtt aaacgtcaat tgagagaagt aattgaacgc
     2221 aaagtttcta aataatattt tgaaaataaa agtttgtaat agatatagac tgtcgatatt
     2281 ggtataagac taatacaacg tcagtattta aatgattagg atttttattt aagaaaggtc
     2341 gtgaatgaag tggccgaaca ctcatttgac gttaatgaag taatcaagga ttttccgata
     2401 ttagatcaaa aagtcaatgg caaacgttta gcatatcttg attcaacagc gacaagtcaa
     2461 acgcctgtgc aagtgttaaa tgttttagaa gattactaca agcgttataa ttcaaacgtt
     2521 catcgtggtg ttcatacatt aggatcattg gcaactgatg gttatgaaaa tgcccgtgaa
     2581 accgttcgtc gttttattaa tgcgaagtat tttgaagaaa tcatttttac acgcggaaca
     2641 actgcgtcga ttaaccttgt agcacatagc tatggtgatg caaatgttga agagggcgat
     2701 gaaattgttg tcactgaaat ggaacatcat gccaatattg ttccttggca acagttagca
     2761 aagcgtaaaa atgcgacatt gaaatttata ccaatgacag ctgacggtga attaaacatc
     2821 gaggatatta agcaaacgat taatgataaa acaaagatcg ttgctattgc acatatatct
     2881 aatgtgctcg gtacaattaa tgatgttaaa accattgcag aaatagctca tcaacatggt
     2941 gcaattatca gtgttgatgg ggcgcaagca gcaccacata tgaaacttga tatgcaagaa
     3001 atgaatgctg atttttatag ttttagtggt cataaaatgc ttggaccaac aggtattggc
     3061 gtattatttg gtaaacgtga gttactacaa aaaatggaac cgattgagtt cggtggcgac
     3121 atgattgatt ttgtaagtaa gtatgatgca acatgggctg atttacctac taaatttgag
     3181 gcgggtactc cattaattgc tcaagcaatt gggcttgcag aagctattcg ctatttagaa
     3241 cgcataggtt ttgatgcaat tcataaatat gaacaagaat taacgatata tgcttatgag
     3301 caaatgtctg caattgaagg aattgaaatt tatggcccgc caaaggatcg tcgtgcaggt
     3361 gtaataacgt ttaatttaca agatgtacat ccacacgatg ttgctacagc cgtagataca
     3421 gaaggtgtag cggttagagc tgggcatcat tgtgcgcaac cgttaatgaa atggttaaat
     3481 gtgtcttcaa cagctagagc gagtttttat atatacaaca cgaaagaaga cgttgatcag
     3541 ttaataaatg ccttgaaaca aacgaaggag tttttctctt atgaatttta ataatctaga
     3601 tcaattatat agatctgtca ttatggatca ttataaaaat cctagaaata aaggtgtatt
     3661 agataacggg tctatgacag tagatatgaa taacccgaca tgcggtgacc gtatacgact
     3721 aacatttgat atagaagacg gcattataaa agatgctaag tttgaaggtg aaggttgttc
     3781 gatttcaatg gcaagtgcat cgatgatgac acaagctgtt aaaggtcatt cacttggaga
     3841 agcaatgcaa atgagccaag aatttacgaa aatgatgctt ggtgaagact atgtgattac
     3901 agaagaaatg ggagatattg aagcattgca aggtgtatct caattcccag ctcgtattaa
     3961 atgtgccaca ttagcttgga aagcattgga aaaaggtact gttgctaaag aaggtaaagc
     4021 agaaggtacg actgaagaag aatagaatgc tgttaatcat agataatttt gatattagac
     4081 atataaaagt ataaaaattt ttataagatg tcatgtcatt gttataatat ggtttacatc
     4141 atgaattaaa aacttacgca cgccgttgta aatatatttt taaggagtga ttgaaatggc
     4201 taaaaaagca cctgatgttg gggattataa atatggattc cacgacgatg atgtgtccat
     4261 tttcagatca gaacgtggtt taactgagaa tatcgttaga gaaatttcta acatgaaaaa
     4321 tgagccggaa tggatgttag atttccgtct taaatcatta aaattgtttt ataaaatgcc
     4381 aatgcctcaa tggggtggcg acttatcaga attgaatttc gatgacatta cttactatgt
     4441 aaagccttca gaacaagctg aacgttcatg ggatgaagtg ccagaagaaa ttaaaagaac
     4501 tttcgataaa ttaggaattc ctgaagctga acaaaaatat ttagctggtg tttctgctca
     4561 atatgaatct gaagttgttt accataatat ggaaaaagaa cttgaagaaa aaggtattat
     4621 ctttaaagat acagatagtg ctttacaaga aaatgaagaa ttattcaaaa aatactttgc
     4681 ttctgtagta cctgcagcag ataacaaatt tgcggcgtta aactcagcag tatggtcagg
     4741 tggttcgttc atttatgtac ctaaaaatat caaactagat acgccactac aagcttattt
     4801 ccgtattaac tctgagaaca tgggtcaatt tgaacgtaca ttaatcattg ctgatgaagg
     4861 tgcttctgta cattacgtag aaggttgtac tgcaccagtt tatacaacta gttctttaca
     4921 ctctgctgtt gtggaaatca ttgtgcataa agatgcgcac gttcgttata ctacgattca
     4981 aaactgggcg aacaatgtat acaatttagt tacaaaacgt acttttgttt atgaaaacgg
     5041 aaatatggaa tgggtagatg gtaacttagg ttctaagtta acgatgaaat atccaaactg
     5101 tgttctttta ggtgaaggtg caaaaggtag tacattatct attgcatttg ctggtaaagg
     5161 acaagttcaa gatgccggtg ctaaaatgat tcataaagca cctaatacat cttctacaat
     5221 tgtttctaaa tctatttcta aaaatggtgg taaagttatt tatcgcggta ttgttcattt
     5281 tggacgtaaa gcaaaaggtg ctcgttcaaa tattgaatgt gatacattaa tcttagataa
     5341 cgaatcaaca tcagatacaa ttccatataa cgaagtattc aacgatcaaa tatcattaga
     5401 acatgaagcc aaggtttcaa aagtttctga agaacaatta ttctatctaa tgagtcgtgg
     5461 tatttctgaa gaagaagcga cagaaatgat tgttatggga ttcatcgaac catttacaaa
     5521 agaacttcca atggaatacg cggtcgaaat gaaccgttta atcaagttcg aaatggaagg
     5581 tagtattggg tagctttaaa ccgcgttgtt aagccattct tgacttccgg aaatggctat
     5641 tgataccatt ttgataccat ttacttgtca aaaatggcta ttgcatcgtg ttttttctga
     5701 gtatataaat ggctgtaagt gcccatcgtt tcagtgattt gagcgtgtct catgagtgac
     5761 tgtaaaacga agatatctac accattattt gcaagataag atgcataaga atgtcttaac
     5821 gcatgaatgt tataatgggg gaaagctttt tggaatttct tttgaacatg actgtaatgt
     5881 ttgggagcca ttcctccgaa aataaaataa ctacgttcat caaaatattt gtttaactct
     5941 ttttcacgtt ggtggcgttc agttaacatt gtattgatga atttaggtaa aggaacaata
     6001 tcctctgaac tatctgtttt tggtctcgga tatatagttc tattagagat gtccattgtt
     6061 ttatttatgg atatctcttt ttttgtattt attgtagtct gtccaaacaa gggccatagc
     6121 ttcgccaatc cttaaacctg tataaaacat taatgtaaat aactctctgt aatcttgctc
     6181 ttcaatgtct ttgattcttt cttcaaattc ttcacgcatc ataaacttag gttttggttt
     6241 tacacgcgga ataggtttaa ttgatattgt tggatctgta cgtaatccaa aatatttttt
     6301 ggcataatta attacaactt taaaacctga ccaaattgta cgagcagaat ttgttgatgc
     6361 tacattctct attagatatt tacgaaactc ttggcattga ttttgtgtta tcttattcat
     6421 ttttatgtgc ccgaacttag ctttaaagtg tttatgatat tcattttgtt tgcgtcgttt
     6481 tgttttaggt ctcaaatcgc tattttctaa atagtgatga aaaacataat caaatgtttt
     6541 tgaatcacta tatccttcgt ttacgtcatt caaaaaaata gcctctgctc tcttagcttc
     6601 acgcttagtt gaaaaaccgc gttgcatctt acgtttgtta ttaccgtata catctttata
     6661 tctaatagaa aaataccatt tacctgtatt atcatcctta tatactggca ttttacttct
     6721 ccctcctcaa aattggcaaa aaaataataa gggtaggcgg gctacccgaa attttattgt
     6781 tgaatcactt cgctattttg acgtttgaaa ttgtcgaaat cattttgtgc tttcttccat
     6841 gaattatagt cttgtccgtc ttgtactgcc catgaaccac ctatgccggc agtatgtcca
     6901 ccattctgac gtttgttttc ttctgttgct cttttagcgt cttgataagc gttataagat
     6961 gtgtcacttg aaaactcatc ttttactggt gcattgttgt ttttattaga agtgggatta
     7021 ttttgtgttt gattttgttt aggtgcgtta ttagtttgtt gatgatcgtt aacatttgtg
     7081 ttgttatctt tgtttacttg attattgtta tcgttttgat tagcattttc ttttttcgct
     7141 tctgctttgt ctttagtttc tttctttttg tctttgttct ctttctttgt ttccgttttc
     7201 ttactttcct ctttcttatc gccgtcgttg ctaccacatg cacctaacac taacgcgcta
     7261 gctaaaatta aatataataa tcttttcatg ttttacactc ctttatttgc tatttgtttt
     7321 aataaatcta tgacttcgtt gttttgctcg ataattctat tattttgctt tattagttcg
     7381 tctcgttgag ctatagagac aaagttttgt tttaattgcg tatcgtagaa tacgaattta
     7441 gcttgtttat ctacgtttgt tgtgaatgta cctaaaccgt tgtagacttt caatagtgta
     7501 gggttaatat tttgcttttg atatgcgtaa gttgtaacgt cggtagcttc tttaatacct
     7561 tgtccgttta aacttttagc tgattttgat tcgtattctt cgttagtgtt tttaaaattt
     7621 tcagatttat aaagttgaat atcaagttct tttccttctt taaaatcatt taatatcttt
     7681 cttttttcat ctgttgtcat ttttttatac atgtcaatct ttctattgct taatttacta
     7741 aacatttttg tttctgttag aatttcttta aaagttaatt tatctcctgc cattttcaat
     7801 ttctccttca ttcgttttat attaaagcgc cacataggcg ctattaatca aaaattcgat
     7861 agttataaat aactttgcct atcacttcga tttcatcaat agaatctaaa tcgtaagaat
     7921 tagttttaaa ttcatctgaa tagcttactg ggtctaaatg tagttttgtt tcagtacgtc
     7981 tcacacgctt aactgtatat tcaccaccta gacgtaatac aaggatgtca ttgctgttaa
     8041 gtttatgatc acaagacttt ctataatcat ggacaattat ataagaaccg ttagcgagta
     8101 ttttattcat gctatctccg tttattttta gtgctataca ttcgctaggt ttacgaccgt
     8161 taaaagcaaa tggtggaact tttaattttt cattatcaat tgcaacttcc tcgaaatttc
     8221 cagcagaaac tttaccgaaa tatggaacct cgatttcgct atcaaattct ggtaaaacaa
     8281 tttcttcaat ttctcctaag agataacctt tagaaacatt gaacaaactt gaaatttttt
     8341 cgaccatacc cattctaggt tcagttcttc cactttccca cattcttata gtaccttcgg
     8401 aaacatctaa ttttctagcc atctcaactt tagacaatct attgttcaat ctgatttctt
     8461 ttatggaatt tttgaaagcc attttgtttt ccttccttat atataatgtt tttacacttt
     8521 tattatacta tgaaaaatcg taattgcaac ccttaaaata cgatttaaca aaataaaaat
     8581 acgtaatttt gaaaaataat tacgaaaaat acttgcaatc gtattttaat tacgatatac
     8641 tttagtcaga acttaacaag gaggtaaaaa aatgaactac atcaaacata gtttgaaatt
     8701 agatgaatgg cgaaaacgaa aaggttacac ccagtcatct ttcgcagaaa aacttggcat
     8761 ttcaccgtct acttataaca tttgggaaaa caacccagaa atgattaaac ctagagatgc
     8821 ttttagaatt gctaagacat tagatatctc tattgatgag attatttttt taaaagatga
     8881 atcgtatttt aaatacgttt tagtagaaga aaaacaaaca atttaatagg aggcattaac
     8941 catgcaacaa gaaagagacc gcttattaaa attaacaaga cttcaactca aatactcgaa
     9001 tctagcgggg caaattgaag cttatgacaa aacacttaaa gaaataagat acactcgaga
     9061 tctttacaac aaatatctaa gcatgaacaa ctatgacgca tttgctggtt tggaaatggt
     9121 tgaagatgaa atcactgaga agcttcgaag tgtcataaaa gaattacaaa aaatagaaaa
     9181 agaaatgaac aagctcaacg gagttgaaag tgataacaaa gttactgatt taacagagtg
     9241 gcggaaagtg aatcagtaac gttcacttta atataaccac gcttatcaac atccacattg
     9301 agcagatgtg agcgagagct ggcgatgata tgagccgcgc ttaaatacat tcgatagtca
     9361 ttgcgataac cgtctgctga atgtgggtgt tgaggaaaaa ggaggatact caaatgcaat
     9421 cattacaaac atttaatttt aaagagctac cagtaagaac agtagaaatt gaaaacgaac
     9481 cttattttgt aggaaaagat attgctgaga ttttaggata tgcaagatca gacaatgcca
     9541 ttagaaatca tgttgatagc gaggacaagc tgacgcacca atttagtgca tcaggtcaaa
     9601 acagaaatat gatcattatc aacgaatcag gattatacag tctaatcttc gatgcttcta
     9661 aacaaagcaa aaacgaaaaa attagagaaa ccgctagaaa attcaaacgc tgggtaacat
     9721 cagatgtcct accagctatt cgcaaacacg gtatatacgc aacagacaat gtaattgaac
     9781 aaacattaaa agatccagac tacatcatta cagtgttgac tgagtataag aaagaaaaag
     9841 agcaaaactt acttttacaa caagaaatcg gagagctaaa acccaaagca gattatgttg
     9901 atgaaatctt aaaatcaact ggcacattag ctacaactca aatcgcggca gactacggta
     9961 tatcagcaca aaagttaaac aaactactac acgaagctag actacaacga aaagtaaata
    10021 aacagtgggt gctttactca gaacacatgg gcaagagtta cacagattca gacactataa
    10081 caattgtgcg ttctgatggc agagaagaca cagttttaca aactagatgg acacaaaaag
    10141 gcagattgaa aatacatgaa atcatgactg aattcggtta tgaagctaat ttagggggag
    10201 cgtaaatgac accagaacaa aaagaaaagc taaacaatat agtattaaca ctttatgcag
    10261 ttaaagaaaa caaaagtcaa acatacacac acaaagatac tcttactgtg acatatgcag
    10321 gcgagattga gcacacttac gaagtcgaca gagagaaaca ccttgaatca atgattgagt
    10381 gggcaattga ccaaatcgaa cagcactttg atttagacga agaagaataa cacacaattg
    10441 aacaaacaac ttaataggag gaattacaaa tgaacgcact atacaaaaca accctcctca
    10501 tcacaatggc agttgtgact tggaaggttt ggaagattga acgaaatacg agaaagcctg
    10561 taatcaatcg aaatgatttt agtaaagagt ctacagcaga aacgattgag cgacacagtg
    10621 atcctgattc aggaataaaa ctacttaagg cattttccga cttcactaaa caagctgaaa
    10681 agcaaaaacc tacactagga gaagtttata gacggaacaa acctgaatta ccaaccgtta
    10741 ctttagacga aaacggactg tttataaatg attttagggt gccttatgta cttgaggaag
    10801 gggttaacgt aaagaaatct atgaacaacc tatataaggt cagtttggac tttttcgcta
    10861 aaagtattat tgcagataat tacgaagcag ataacccaga gaatcaacag ttattttaaa
    10921 ggaggaatag atatgataaa aaatagtttg caagctaaag aacttgcggt aattttatct
    10981 gtttctaaat ccaaagcggg acaaataata agagaactga ataaagaact tgaagacgaa
    11041 gggtacattg cgatacgagg cagaataccc gtccaattag ctaggaaaaa attcccttat
    11101 cacggcttgt cagacgagag aataatggag gcgttgaaaa aagaaaatga gtaaaactta
    11161 taaaagctac ctattagcag tattgtgctt cacagtctta gcgattgtac ttatgccgtt
    11221 tctatacttc actacagcat ggtcaattgc gggattcgca agtatcgcaa cattcatatt
    11281 ctataaggaa tacttttatg gggtggatga ttaaatgact tggtttgaag aatacgttaa
    11341 acctagtgtg gaatgggaaa gaaaggcaga acaagctgtt ttaagtgatg atgaagttaa
    11401 aacgatcact gaatatagaa agaagtacaa caacccgcat atttacatgt cggctcagaa
    11461 cagaaattat cttgttgaat atttagatag acatactgga gacatagtat tacacaattc
    11521 aaaacttaag aaatcatcca gaagaagagt gcatcaatat ttaatggtcg gccaaatagt
    11581 agtgccgggc gaaccaaaag gcacaattta tgaagcatct ctgataataa gataaaaaaa
    11641 ctgctacttg cgccaacaag taacagagac aaacgattag caaaattaat tcacgttcaa
    11701 tataaaacga aaaacggagg aagtcaagat gtattacgaa ataggcgatg tatgtcagaa
    11761 ggtaattaat gtagacggat ttgattttaa attagcagtt aagaagaagg accacagcat
    11821 tctggtgaat atcttagatt tagaagataa gtttatcgac ggcataaaca taactaatga
    11881 gaacgatcta tacacagcat tagacatatt aaatcaatct atttacgaat ggattgaaga
    11941 aaacgcagat gattatgaca gactaattaa cttagtcatg aaatggtagg tataagcatg
    12001 agagatacag aaagaaatat attgaatatt tttaa
//
LOCUS       DAMZLE010000077        12006 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMZLE010000077 DAMZLE010000000
VERSION     DAMZLE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.077"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            8..442
                     /locus_tag="POB92_001779"
     CDS             8..442
                     /locus_tag="POB92_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDI6579264.1"
                     /translation="MVYYLLNIDLGKMIVAQDLNLQEIFFHNLIYFFSAIFGFLTLGL
                     LNIGMLLINSGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIASTFSLGFLSYL
                     YQRMVKKNKIKNKMFHNFVTTIITVIVLTIIASLLEVYVTIN"
     gene            454..1176
                     /locus_tag="POB92_001780"
     CDS             454..1176
                     /locus_tag="POB92_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579265.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQITIEKTIKDVGLNIEDLNIPVYNFSSGMKQKLNFASMLLVDSNVLLLDEPFN
                     ALDHVAQKNFTRILKGLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSF
                     NQTTLETWLLESQKEVIPYEEK"
     gene            1342..1539
                     /locus_tag="POB92_001781"
     CDS             1342..1539
                     /locus_tag="POB92_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6579266.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
     gene            complement(2049..3641)
                     /locus_tag="POB92_001782"
     CDS             complement(2049..3641)
                     /locus_tag="POB92_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579267.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLGIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            3598..5076
                     /locus_tag="POB92_001783"
     CDS             3598..5076
                     /locus_tag="POB92_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579268.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     ERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            5082..6245
                     /locus_tag="POB92_001784"
     CDS             5082..6245
                     /locus_tag="POB92_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579269.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            6262..8037
                     /locus_tag="POB92_001785"
     CDS             6262..8037
                     /locus_tag="POB92_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6579270.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            8075..10081
                     /gene="ggt"
                     /locus_tag="POB92_001786"
     CDS             8075..10081
                     /gene="ggt"
                     /locus_tag="POB92_001786"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDI6579271.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYYETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDNYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRVYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLLSPEIYDNDTH
                     RVYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIA
                     WLARKKGYQVALKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(10394..11167)
                     /locus_tag="POB92_001787"
     CDS             complement(10394..11167)
                     /locus_tag="POB92_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6579272.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPRNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKLSEH"
     gene            complement(11357..11983)
                     /locus_tag="POB92_001788"
     CDS             complement(11357..11983)
                     /locus_tag="POB92_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI6579273.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATIF"
BASE COUNT         4044 a         1816 c         2136 g         4010 t
ORIGIN      
        1 gggatgcttg gtttattact tattaaatat tgatttaggt aaaatgatag ttgctcaaga
       61 tttaaattta caagagattt tctttcataa tttaatttat ttcttctctg caatatttgg
      121 ttttctgaca cttggtctgt taaatatagg tatgttactc ataaattctg ggatgttagg
      181 cttttttata gcacatggaa tctatagcaa tcaattaaaa tctattctat tagtattagc
      241 gccacacgct atatttgaga ttttagcact tttaattgct tcaacattct cattaggttt
      301 tttatcatac ctttatcaaa gaatggtaaa gaaaaataaa ataaaaaata agatgttcca
      361 taattttgta acaacaataa taactgttat tgtattaaca ataatcgcat cattattaga
      421 agtttatgta accataaatt aaggaagtgt atcatgctag ttttaaataa tcttcaaaaa
      481 aaatttacta attcaaaaat ggttttgaat aatgtgaatg ctaagtttag aagtgggagc
      541 atcaattgca tagtgggagt aaatggggca ggtaaaacga ctttgttaaa tattattagt
      601 agtatattga tgccaacaaa aggtgatgta tatttaaata gtgaaagtat ttttgaaaat
      661 agcacactaa aaaaagagat tttttacaca ccagtaaatc ccttttttta tgaaaattta
      721 agtgctaaag ataatttgta cttaatatgt agcctatata atcggaaaat agatcaaata
      781 actattgaga aaactattaa agatgtaggt ttgaatatag aagatctaaa tatacctgta
      841 tataattttt caagtggtat gaagcaaaaa ctaaattttg cttctatgtt attagtagat
      901 tcaaatgttt tattattaga tgaaccattt aatgcccttg accatgttgc tcaaaaaaat
      961 ttcacacgaa ttttaaaggg attagtttct aaagaaaaga taataatttt cactagtcat
     1021 ttgcctaata caattttaga actctcagaa aatatttatt tgttaaaaga tggatgtttt
     1081 acagataaac gaaaagcaca gtcatttaat caaaccacgt tagaaacatg gttgttagaa
     1141 agtcaaaaag aggtgatacc ttatgaagaa aaatgatttg ttagtaatga gtgtagtata
     1201 tattttaact tcagtcataa tgttctacat taatggatta agtaaacaat tttttacata
     1261 tgtagttggt ttcccaatta tttattttgg ttatatatgt ctagttaatc ttataaaaaa
     1321 aagaagccgg aataattaaa agtgaaaaat aatttgaaaa tagctagagt ttcactatct
     1381 atgactcaaa aggagctagc aaataaagtt ggagtaacta gacaaacaat atctcttata
     1441 gaaaagggtg tacataaccc ctctctatca ctatgtaaaa atatttgttc ggtgttgaat
     1501 aaaaatttgg atgagatatt tggagagaaa ccacaataaa agagcttatt gaaagataat
     1561 ctaaaagtcg acaatatatt tcttctcaga aactgctatt attttgaaaa gaaaacacac
     1621 gtattataaa tttaaaatac cgaaatatag cattattaaa tcaaaaatag aattaaaaaa
     1681 gaaaattaat cgagaaaagt attaaatgtc atttatttga atgtgttaat tatgaaagaa
     1741 aaagagtaag tgacgttaaa tatacattga ctaagctaag ttgacacact caaaaatctg
     1801 aatattctaa taaaatataa ataatccccc gcacttcaca tagtggcggg ggatttattc
     1861 tggcgtcaaa atgcccaaag tatgggggtg actcttaaat catggcaaag cacctaaaac
     1921 ttgatatggt gccgagccct aacttagcac aatatagtta gtatgacaaa gtcatctaac
     1981 ataacacatc agagattgcc tgatttattc cagaccatac agtgatacag ttgcacaacg
     2041 catgactttt atttagcaat aactgctact tctgaaataa gttgctttgc atagtctgat
     2101 tgcggatgtt tgataatatc ttctgtgtta ttcagttcaa cgatttcgcc atttttcata
     2161 actgcaacgc gatcacatat ttcattgata acacccatgt catgtgtgat gaataaataa
     2221 gtgatgccga agtctaactg taattgtttt aataactcga tgatatcttt ttgaattgaa
     2281 acgtctaaag cggacactgc ctcgtcgcaa acaatcactt taggttctac agcaagtgct
     2341 ctcgcgatac ttacacgctg acgttgccca ccagataatt cgtgtggata gcgatataag
     2401 aaactttgat ctaggccaac cttttctaac aacgatacga cagttttaat aatgtcatca
     2461 ttatctttga ctttcccatg aatgattagt ggtcgtttaa tcacatcaat gactttaaat
     2521 cttggattaa tagatgcgaa tggatcttga aaaatcattt gtatctcttg tcgtaaagat
     2581 ttcaattcat catctttaaa taaacttaat ggtaattcgt tataccaaat aaagccttct
     2641 gacacttcct ttagaccgac gaccgtctta gctaatgtcg atttccctga ccctgattca
     2701 ccgacaatgc ctaatgtttc gccttttcta atacccaagt taatatcatt aactgctcgg
     2761 tataggctgc cactcggtga tgtgtaatcc acgctcacgc gatcgaattt taataaaata
     2821 tcattgttta acggtcttgg cggacgcgtt tgatgaatat caggaatcgc atctattaag
     2881 cgttttgtat aggtatgttg tggcgattta aaaatacttt caaccgtgcc actttcaacg
     2941 acacttccat ctttcattac aatcacatcg tcgcaaaatt gatacacagc gcctaaatcg
     3001 tgagtgataa aaataataga tgtttctgtg tactcataaa gggacttcat taactgcagt
     3061 aattgatttt gtgtactggc atctaatgcc gttgttggtt catctgcgat taaaatttgt
     3121 ggctttaaaa tcaatgccat tgctatcatg acacgttgac gcataccacc agaaagttca
     3181 tgtggataag catcaaattg tcgagttgca tgttttatac ctactttttc taaaatgtct
     3241 attgtcatcg actttgcttc agatttagat acacgtttat gttgaaatag tacttctgta
     3301 atttgtttgc caatcgttaa tcttggattc aacgaagaga gtggatcttg aaaaatcatt
     3361 gaaatatcct tacctcgaat ttgttgtaac gctgaagttg ataaattatt taacgattgc
     3421 ccattaaaaa taatttctcc tgttaatgtg tgatctggat aatctggtag tagccctaaa
     3481 atagatttag cagtaatact ttttcctgat cctgattcac caacaatacc taggatatgt
     3541 ttttttcgta attcgaaaga gacatttttt accgcttgaa ctgtagtttc atcataattg
     3601 aattgtacat tcagactgtt gacttctaat aaatttgaca tgatgttgtg cctccctatt
     3661 cacaaatgtg taaatttatt ctatactttt gtatatcata gtaatctact caagattatt
     3721 ttacaaggag tgattatcat aattacttat ttagaatctt ttaagtcgtt gtatcacaag
     3781 gcattttaca agtttgtatt atcggtatta agcttgccaa tatttttata tgcagtgata
     3841 aagtttttct tttctgctaa gagaaaaaat ttttacgcta ataattctga aatttcagaa
     3901 attgaacgag cgttacatca aaaatataaa tatttatcgc agcaaaagtc atccacacaa
     3961 atacataaag aagcattaaa aatattcaag gcacaaagtt ctaatacgag ttcaaagaat
     4021 attgaacaag cacatttttc aacatacttt gaaaatttat tatttcataa gttcatcatg
     4081 atcaaagtaa tattggcctt gccgatgttc atcttattga ctatttattt acagccatta
     4141 gttagatata tttttgagag aattgtcatg gctgtgattg tcatcattgg tgttattgtc
     4201 agtgtgttta ccattctgta tttttcaccg cttgatgcgg cttatagcat actgggacaa
     4261 aatgcaacaa aggcacagat acatcaattc aatgtattac atcatcttaa cgaaccttat
     4321 tttattcaat tgtgggatac catcaagggt gtttttacct ttgacttagg tacgacttac
     4381 aaagggaatg aggttgtgac taaagcagtt ggcgaaagaa ttccaattac aataattgtc
     4441 gcagtattag cgctaattgt ggcattaatt attgcaatac caattggtat tatcagtgcg
     4501 atgaagcgaa atagttggct tgatatcacg ttaatgataa ttgcattaat tggtttatct
     4561 attccaagtt tctggcaagg gctattattc attttagcgt tctcattgaa attggatatt
     4621 ttgccaccat cttatatgcc agaacatcca atatcgttga ttttacctgt acttgtcatt
     4681 ggaacaagta ttgctgcttc tatcacgcgt atgacaaggt cttctgtact tgaagtaatg
     4741 cgcagcgatt atgttttaac tgcttatgca aaaggattat cgacgacaca agttgttatt
     4801 aaacatattt tgaaaaatgc cattattcca attgtaacgt tagttggtct tctagtggca
     4861 gagttactag gcggttcagc agtgacggaa caagtattta acattaatgg tatcgggcgt
     4921 tatatcgtcc aaaaacaact aatacctgat atcccagcag tcatgggtgg ggtcgtatat
     4981 atatcaattg taatatcttt agcaaactta attattgata tattttatgc tttaatcgat
     5041 ccaaaattac gtagtgaaat taacgaaagg aagtgaggca tatggtaaaa cttacaacaa
     5101 agatagcttc cttaaaacta ttcgcaagtt atgctatagc aacttatatt ttagttatat
     5161 taacgagtgc attaaatctt tttaaaggtt atgtggccga tacgttctat attgctgaaa
     5221 cattgctaat cgttttaacc atcattttaa ttatcatttt aacaacggaa caaacatgga
     5281 agcatcatga cttatggcga cgtatcgtcg aagtgttgtt attgttgatg acattaacag
     5341 gcaacgtatt tacattatta atgtttgtaa gtattagacg ttaccaacgt acatcgcaaa
     5401 tacatagtta taacgggtgg gaatcgttta tacgaaaaac tactagacat cgtattgcga
     5461 ttatcgggtt acttatttta gtctacatgc tgacattatc aattgtgtca caatttacat
     5521 ttgatacgac attggctact aaaaatcagt tcaatgcact gttacatgga ccgagtctag
     5581 cctatccgtt tggtactgat gatttcggta gagacttatt tacacgcgta gttgtaggaa
     5641 cgaagctgac attttcaatt tcaattattt cagtagttat tgcagttatt tttggtgtgt
     5701 tactaggcac tatcgcaggt tattttaatc atattgataa tttaataatg cgaattttag
     5761 atgtagtgtt tgcaattcca tcattattgt tagcggtggc aattattgca tcatttggag
     5821 caagtattcc aaatttaatt attgctttaa gtatcggtaa tataccatca tttgcacgga
     5881 caatgcgtgc cagtgtttta gaaattaaac gcatggaata tgtagatgca gcacgtatca
     5941 ctggtgaaaa cacttggaat atcatatggc gttatatttt accgaatgcg attgcgccta
     6001 tgattgtacg tttttcatta aatataggtg tggttgtatt aacaacaagt agtttaagtt
     6061 tcctaggact tggtgttgca cctgatgtag ctgaatgggg caacatttta cgtaccggta
     6121 gtaactactt ggaaacgcac agtaatttag ctattgtacc tggtgtttgt attatgttcg
     6181 tcgttttagc atttaatttt ataggtgatg cagtgcgtga tgcactagat ccaagaattc
     6241 attaaaaagg tagggataga tgtgaagaaa atcattagta tcgcaattat agttttagcg
     6301 ttggtattaa gtggttgtgg tgtccctacg aaatcagaag tggctcaaaa gtcatcgaaa
     6361 gttgaagtga aaggcgagcg accaacaata catttcctag gacaagcaag ttatgaaaat
     6421 gatatgaata tcgttaaaga tcaattggaa aatgcaggat ttaacgtgaa gatgaatatc
     6481 caaccagatt atggtagcta tcgtacacaa cgtcaagccg gcaattatga tatccaaatt
     6541 gatgactgga tgacagtgtt tggtgacccg aactatgcta tgacggcatt atttagttct
     6601 acaggatcaa atagtttatt gaaagataaa catgtagacc agttgttaaa taaagcttct
     6661 actcaaaatg aagcagatgt taaacaaaca tataagcaaa ttgaagatga agttgtattt
     6721 gataaagggt atatggcgcc tttatatgga tcaaaaaaga atttagtata tgacaataaa
     6781 gtgttagata aaaatagtgt tggattgcca aattcacgtg cattaatatg gcaacaattt
     6841 gattacaaca atagtagaga acgagatacg cggccacttg tgatgacaca acaagatggt
     6901 gaaattccta cattggatcc aatacgttca attgcgccgt cagtatattc aattaatatg
     6961 aatatgtaca caaggttatt attattagat gaaaatgatc atttaacaac gaaaggttcg
     7021 ttaagtcatg attatgctgt gaataaggac aataaagcat tttatttctt gttaagagat
     7081 gatgattatt ttgcgaaagt ggtcaatgga caagcacgta atactggaga gcgtgtatcg
     7141 gctgaagatg ttaagttttc tttagataga gcacgtgata aaaagtctgt gcctaacaat
     7201 aatacttaca atatgcacaa acatataaat gacatcaaga tattaaaaga tgaggacatc
     7261 gatcagttgc gtaaagagaa agacaaggac gataaatcaa tctatgataa gttgattaaa
     7321 gcttataacg tcaaatcgtt aacgacagat ggtcaaaaag taaataataa agacggtatt
     7381 tatcaaattg ttaaaattac gacagatcaa tcgatgcctc gagaggtaaa ttacttaaca
     7441 cactcttcgg caggcatttt atctaaaaaa tttgttaatc aagtaaatca agaatatcca
     7501 aaaggatatg gggatagcag tacaattcct gcaaattcag atgggaaaaa tgcgctgtat
     7561 gcaagtggcg catacattat gacacagaaa aatgcatatc aagcaacgtt tcaacgtaat
     7621 ccaggattca acgaaacaga aaaaggtagt tatggaccag ctaaaattaa aaatattaca
     7681 ttgaagttta atggtgaccc gaataatgca ttgtcagaac ttagaaatca ttcaattgat
     7741 atgttggcag atgtgaatca aaaacatttt gatttaatta agtcggataa aaatttaagc
     7801 attattcgca aaaatggacg caagtcagtc tttttaatgc taaatattaa aaaaggtata
     7861 tttaagacgc atccaaactt gagacaagca gtagttaatg cgatagatca ggatcaattt
     7921 attaagtttt atcgtggcga taaatttaaa attgcatcac cgattacacc acttgtcgat
     7981 actggtaacg agcaacgtca agatttagaa aaagtagaaa aagccatcaa tcaataatgt
     8041 tttaaatatt taacagaaag taggaggata tagtatggtc attaacttaa atgacaaaca
     8101 gacaaaaaca tctaaagaag ggttaatttc cgtatcacat cctcttgcgg ctaaaattgg
     8161 taaggatgta ttagatcaag gtggcaacgc catggatgca gtgattgcaa ttcaactggc
     8221 gttgaatgtg gtagaaccat ttgcatcagg tattggtggt ggcgggtatt tgctatatta
     8281 tgagcaaagt actggcagta taactgcgtt tgatgcgcgt gagacagccc ctgcacatgt
     8341 agataaacaa ttttatctag atgattcagg cgaatataaa tcattttttg atatgactac
     8401 acatggtaaa actgtcgctg tgccagcaat tccaaagctg tttgattata ttcacaagcg
     8461 ttatgctaag ttgtcattgg aagatttaat taatcctgca attgaactag ctattgaagg
     8521 tcatgcagcc aattgggcta ctgaaaaata ttcgcgccag caacacgcac gattgacaaa
     8581 gtattatgaa acggcacaag tatttacgca tgaaaatcaa tattggcgtg aaggtgattg
     8641 gattgtacaa cccgaattag gtaagacatt tcaaatatta agagaacaag ggtttaatgc
     8701 attttataaa ggtgacattg cgaaacaatt agtcaatgtt gtcaaagcat gtggtgggac
     8761 aatcacttta gaggatctag ccaaatatga cattcagatt aaagcgccaa tcagtgcaac
     8821 atttaaagac tatgacattt attcaatggg accatctagt tctggcggta tcacggtaat
     8881 tcaaatattg aagttattag aacatgtcga tttaccatct atgggtccaa gatctgttga
     8941 ttacttgcat catttgatac aagcgatgca tttagcatat agtgatcgtg cgcaatattt
     9001 ggcggatgat aattttcatg aggtgcctgt acagtcatta attgatgaca attatttaaa
     9061 agcacgcagt acgctcattg atagcaataa agcaaatatt gatatagagc atggtgttgt
     9121 gtctgattgc attagtcata cagatgttga agaaaatcat accgaaacaa ctcatttttg
     9181 tgtgattgat aaggaaggta atattgcttc atttacgaca tcaatcggta tgatttacgg
     9241 atcgggtatc acgattccag gctacggcgt gttattgaat acgacaatgg atggctttga
     9301 tgtagtagat ggtggtatta acgaaattgc accatataaa cgaccactaa gtaacatggc
     9361 tccaacgatt gtgatgtatc acgggaagcc aatattaaca gtaggtgcac ctggtgccat
     9421 aagtatcatt gctagtgttg cgcaaacatt aatcaatgta ttagtgtttg gcatggatat
     9481 tcagcaggct atagatgaac ctagagttta tagtagccat cctaatcgca ttgaatggga
     9541 gcctcaattt tcacaatcta caatattagc attgattgca cgtggacatg caatggaaca
     9601 taaaccagat gcctatattg gagatgtaca tggactacat gttgacttga atacacgtga
     9661 cgcatcggga ggtgctgatg atacgaggga aggtacagtg atgggtggtg aggtattatc
     9721 aattagaaaa caaccattac ttagtcccga aatatacgat aatgacaccc atcgtgtata
     9781 tttcaatgat gtgcagttac ctttattggc agatcaagtg cgatggatgc atgacaaata
     9841 ttgggttgat gaaagtgtcg ttagaatcat cttctctgaa gttagtgcac atattgaaga
     9901 tttaagaagt tatgaaaacg caggagaaaa ttatatagat attgcctggt tagcacgaaa
     9961 gaaaggttat caagtggcat tgaaagatga tggtttatac ttaactgatg atacatatac
    10021 ttcagtgaaa cggaacacaa atgcatacta tagatatgat cgagatagta tcacaagata
    10081 gtttatacgc ttgatatgaa gtttgtagtg gataagggct tttgttgaat gaaaggattc
    10141 gattttgaac tttagcgtga cgcttgagtg tagtaatgtt gaagttgtta taagaaacgc
    10201 tagtcgcctt tgtaaaatat aaaacgcccc acaaattcgt ttttgatgta caccacaata
    10261 gttagtctaa aatctaactt gatgtgattc ggtacaatta aatgaatgat gtgaggcgtt
    10321 tttataatgt gtgattttag tatgttaaac gataataaat gatttggtta tttctataat
    10381 tacatcataa gtattaatgt tcacttaatt tattataagt aaggctttct ttgtatataa
    10441 tttggtcttt tccaggactt gaaaaataaa aataaagttt ctgatcttga tgattattgc
    10501 cttttaaatc actcattccc tttaattcca cctttgttga tgcatttctg ggaatattat
    10561 atctacgttt taattgttta acgtttggat tatcattcga caacttacca gataaattat
    10621 aataatcagt ggtaggatca tgtgtgactt tcaaatcatt gctatttaaa atgcttgtta
    10681 aatcaccact ttgaatcaaa aattgattgt tttcgatttt ttgtttcagc gcgggatctt
    10741 ttacgtcttt tgtgaaaacg attttattat taactacttt tactggataa cttttgtatg
    10801 tcgagtcagt agcatttttt ctatcgtttg tagttgtgtc atattcacca gttattttat
    10861 gtgtgttctt atctaccttt aacaacatac ggtcttcttt taaaagctca tctgatccaa
    10921 caactgaata agaggattct atataccatg tgtcttgatc attattttca taatggggat
    10981 tatcgtgacc atcaatttca taaagcgttt ctaagttttt aataggatac gtacttagta
    11041 cttttttaag accatctttc aaatgaattt gttcccactt cattgccaaa aacataccgc
    11101 cactgactac aattgaaata ataataattg ctgctaagtt taaccagaaa attttatgtg
    11161 ctttcataca ttcacacctt ttcttaaaat acttaattaa cactataata atatatttta
    11221 aaaatattta catcagtatt aaagtgaata tcaaacttta aatgtgtgga aataataggt
    11281 atttataaaa aagtaggaaa tgcatacaat taaaaactgt gtgacgtttg agacgtcaca
    11341 cagtgtaact aaaaatttaa aaaattgttg ctaatttttc agcattatta atactagttg
    11401 ctttaatttc ttcagtctta tgaggttcag cattgtgtcc ttcaataatg attgtttcat
    11461 atgatggcac acctaagaat gtcataattg ttcttaaata acggtcaccc atttcaaaat
    11521 cagcagcagg tccttcagta taatatccac cacgtgattg aatgtgtaat acttttttgt
    11581 cagttagtaa accttgtggt ccttcagcag aatatttaaa agttttacct gcaattgaaa
    11641 tagcatcaat atatgcttta actacaggtg ggaaagaaag gttccacata ggcgttacaa
    11701 atacatattt atctgcactt aaaaattctt ctaaaatgtc actcaatctt gaaactttca
    11761 tttgttcatc atcagttaac gtttcgccat tactcatttt tccccaacca gttaatacat
    11821 ctttgtcaat aactggaata taagtttcaa ataaatcaat atgtttcact tcatcatcag
    11881 gatgttgttg ttgatatgtt tcgataaatg ctttaccagc cgccatagaa tttgatacca
    11941 gttcattaaa agggtgtgct gtaatatata atacttttgc catttgaaaa ttctcctctg
    12001 tttctg
//
LOCUS       DAMZLE010000078        11974 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMZLE010000078 DAMZLE010000000
VERSION     DAMZLE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.078"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            306..1706
                     /locus_tag="POB92_001789"
     CDS             306..1706
                     /locus_tag="POB92_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579274.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYIDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2250..3626
                     /locus_tag="POB92_001790"
     CDS             2250..3626
                     /locus_tag="POB92_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6579275.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3628..4767
                     /locus_tag="POB92_001791"
     CDS             3628..4767
                     /locus_tag="POB92_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDI6579276.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4742..5107
                     /locus_tag="POB92_001792"
     CDS             4742..5107
                     /locus_tag="POB92_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDI6579277.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            5110..5379
                     /locus_tag="POB92_001793"
     CDS             5110..5379
                     /locus_tag="POB92_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579278.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5580..5747)
                     /locus_tag="POB92_001794"
     CDS             complement(5580..5747)
                     /locus_tag="POB92_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDI6579279.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(6254..7084)
                     /gene="thiD"
                     /locus_tag="POB92_001795"
     CDS             complement(6254..7084)
                     /gene="thiD"
                     /locus_tag="POB92_001795"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6579280.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            7268..7924
                     /locus_tag="POB92_001796"
     CDS             7268..7924
                     /locus_tag="POB92_001796"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI6579281.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKLPINWCESEA
                     "
     gene            7925..8305
                     /locus_tag="POB92_001797"
     CDS             7925..8305
                     /locus_tag="POB92_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDI6579282.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            8438..8806
                     /locus_tag="POB92_001798"
     CDS             8438..8806
                     /locus_tag="POB92_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDI6579283.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            8927..10411
                     /locus_tag="POB92_001799"
     CDS             8927..10411
                     /locus_tag="POB92_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6579284.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGTLLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(10552..11004)
                     /locus_tag="POB92_001800"
     CDS             complement(10552..11004)
                     /locus_tag="POB92_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI6579285.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(11017..11781)
                     /locus_tag="POB92_001801"
     CDS             complement(11017..11781)
                     /locus_tag="POB92_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6579286.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
BASE COUNT         4053 a         1832 c         2173 g         3916 t
ORIGIN      
        1 gtaaattgag tataaacatt taaatacgat gtcgaaaatg gcgatagcat atgacttaca
       61 tgaagttgtg tgctatcgct atttttagtt ataattccaa aaagttaatc gttcgatgat
      121 ttaagaatta ttattgttta attcaaatgt atgagggtat aaaatcattg aatttaattc
      181 gataaagcga aatttttgaa caaacatact tttgtattta tataaaagtt ttaattctta
      241 taaatttgac aaaactaatt aactccgtat aattatgaaa catacaagag ggagtgtatg
      301 aattcatgga ttttaataaa gagaatatta acatggtgga tgcaaagaaa gctaaaaaaa
      361 ccgttgttgc aaccggtatc ggtaatgcaa tggaatggtt cgattttggt gtctatgcat
      421 atacaactgc gtacattgga gcgaacttct tctctccagt agagaatgca gacattcgac
      481 aaatgttgac tttcgcagca ttagccattg cgtttttatt aagaccaatt ggtggtgtcg
      541 tatttggtat tattggtgac aaatatggac gtaaagttgt attaacatct acaattattt
      601 taatggcatt ttcaacatta accattggat tattgccaag ctatgatcaa attggacttt
      661 gggcaccaat actattattg cttgcaagag tactacaagg gttttcaaca ggtggagagt
      721 atgcgggggc aatgacatat gttgccgaat catctccaga taagcgtcgt aactcattag
      781 gtagtggact agaaattggg acattatcag gttacatagc tgcttcaatt atgattgctg
      841 tattaacatt ctttttaaca gatgaacaaa tggcatcatt tggttggaga atcccattct
      901 tactcggttt attcctagga ttattcggct tatatttacg tcgtaagctg gaagaatcac
      961 cagttttcga aaatgatgtt gcaacacaac cagaaagaga taacattaac tttttacaaa
     1021 tcatcagatt ttattacata gatatatttg tatgttttgt agctgttgta ttcttcaatg
     1081 ttacaaacta tatggtaact gcatatttac caacctattt agaacaagtt attaaattag
     1141 atgcaacgac aacaagtgta ttaattactt gtgtcatggc aataatgatt ccattagcat
     1201 taatgtttgg taagttagcg gataaaatag gtgaaaagaa agtatttcta attggtactg
     1261 gtgggctaac attattcagt atcatcgcat ttatgttatt acattcacaa tcatttgttg
     1321 taatagtaat cggtatattt atattaggat ttttcttatc aacttacgaa gcgacaatgc
     1381 cagggtcgtt accaacgatg ttttacagtc atataagata tcgaacttta tcagtaacat
     1441 ttaatatctc tgtttcgata tttggtggta cgacgccatt agttgcaaca tggttagtta
     1501 cgaaaactgg agatccatta gcacctgcgt attatttaac agcaatcagt gttattggct
     1561 ttttagttat tacattctta catttaagta cagcaggaaa atctctaaaa ggttcgtatc
     1621 caaatgtaga taacgagcaa gatagagctt attatgcaga acatccaaaa gaagcattat
     1681 ggtgggttaa agaacgtaag aattagagat tttaataaaa agtataaatc aatcgtatat
     1741 aagcacttta aagctagtag gttctgctaa ctttaaagtg ctttttaaat tgagaactgt
     1801 aattagccgt aataaagttt ttgtatatac ataaaccccc actgcaatga ttatcgcaat
     1861 gggggaaaga ggggacttaa agcatatgtt tagctttgaa tacttaaaat tctcttgcta
     1921 ttgaaatgtt aggatgtaaa tatgtcttag agtattttgt ccaacgcaat taatattgag
     1981 actctaacct tcaatattat tatagagaac acaaacttaa atagattggg tgacttattt
     2041 gtgtcagtta ttgcgattgc gataacttct tttctctata tacatatagt aacgtcttat
     2101 ctaataaaaa acatggtact acagtatcaa atttatctag ggcttaagtt tgatttttat
     2161 aataggcagg tttacctgat aaaaatactt attcattata taatgttaac aatatgtatt
     2221 ttaaagttta cattgagtga gggatattga tgaacgtaat tttagaacag ttgaaaacac
     2281 atactcaaaa taaacctaat gacatagcat tacatatcga tgatgaaaca attacatata
     2341 gtcaactaaa tgcccgcatc actagcgcag ttgaatcttt gcagaaatat tcacttaacc
     2401 ctgtcgttgc tattaatatg aaatcaccgg tgcaaagtat tatttgttat ttagctttgc
     2461 atcgtttaca taaagtgcct atgatgatgg aaggtaaatg gcaaagtact atacatcgtc
     2521 aattgattga aaaatatggt attaaagatg taattggaga tacaggtctc atgcagaata
     2581 tagactcacc gatgtttatt gattcaacgc aattacagca ctaccccaat ttattacata
     2641 ttggttttac ttcagggaca actggactgc caaaagcata ttatcgtgat gaagattcat
     2701 ggttggcttc ttttgaagtt aatgaaatgt tgatgttaaa aaatgaaaat gcaatagcag
     2761 cccctggacc actatcgcac tcgttaacat tatatgcgtt attgtttgct ttaagttccg
     2821 gtcgtacttt tataggacag accacttttc atcctgaaaa gttacttaat caatgtcata
     2881 aaatatcatc atacaaagtt gctatgtttc ttgttccaac gatgattaaa tcattattgt
     2941 tagtttacaa caatgaacat acaatccaat cattttttag cagtggagat aagctgcatt
     3001 cttctatttt taaaaagata aaaaatcaag caaatgacat aaatttgatt gaattttttg
     3061 gtacatcgga aaccagtttt atcagctata acttgaatca gcaagcacca gttgaatcag
     3121 taggtgtgct atttccaaat gtggaattga aaacaacgaa tcacgatcac aatggtatag
     3181 gaactatttg tataaaaagt aatatgatgt ttagtggcta tgtaagtgaa caatgtataa
     3241 ataatgatga atggtttgtt actaatgata atggctatgt aaaagagcag tatttatatt
     3301 taacgggacg tcaacaggat atgttaatta ttggtggtca aaatatatat ccagcacatg
     3361 ttgaacgcct tttaacgcaa tcttcgagca ttgatgaagc aattatcatc ggtattccaa
     3421 atgagcgttt tggtcaaata ggcgtattgc tttattctgg tgatgtgaca cttacacata
     3481 aaaatgtaaa acaattttta aaaaagaaag tgaaacgcta tgaaattcca tcgatgattc
     3541 atcatgtaga aaagatgtat tacactgcaa gtggtaaaat tgctagagaa aaaatgatgt
     3601 cgatgtattt gagaggtgaa ttataatatg aatcaagcag tcatagttgc agctaaacga
     3661 actgcatttg ggaaatatgg tggcacttta aaacatttag agccagaaca attgcttaaa
     3721 cctttattcc aacattttaa agagaagtat ccagaggtaa tatctaaaat agatgatgta
     3781 gttttaggta atgttgttgg gaatggtggc aatattgcaa gaaaagcatt gcttgaagcg
     3841 gggcttaaag attcaatacc tggcgtcaca atcgatcggc aatgtgggtc tggacttgaa
     3901 agtgttcaat atgcatgtcg catgatccaa gccggagctg gcaaggtata tattgcaggt
     3961 ggtgttgaaa gtacaagtcg agcaccttgg aaaatcaaac gaccgcattc tgtgtacgaa
     4021 acagcattac ctgagtttta tgagcgtgca tcatttgcac ctgaaatgag cgacccatca
     4081 atgattcaag gtgctgaaaa tgtggccaag atgtatgatg tttcaagaga attacaagat
     4141 gaatttgctt atcgaagtca tcaattgaca gcggaaaatg taaagaatgg aaatatttct
     4201 caggaaatat tacctataac cgttaaagga gaaatattca acactgatga aagtctaaaa
     4261 tcacatattc cgaaagataa ctttggccga tttaagcccg tgatcaaagg tgggaccgtt
     4321 accgctgcga atagttgtat gaaaaatgat ggtgcagttt tattgcttat tatggaaaaa
     4381 gatatggcat acgaattagg tttcgagcat ggtttattat ttaaagatgg tgttacggta
     4441 ggtgttgatt ctaattttcc tggcattggt ccagtaccag ccatttccaa cttactaaaa
     4501 agaaatcaat taacgataga aaatattgaa gtcattgaaa ttaacgaagc gttcagtgca
     4561 caggtagttg cctgccaaca agctttaaat atttcaaata cgcaattaaa tatatggggt
     4621 ggtgcattag catcaggtca tccatacggt gcaagcggtg cccaattagt gactcgatta
     4681 ttttatatgt ttgacaaaga gactatgatt gcatctatgg ggataggggg aggtctagga
     4741 aatgcagcat tatttactcg attctaacca gcgattaaat gtgtcatttt ctaaggatag
     4801 tgtggctgca tattatcagt gttttaacca accttataga aaagaagtac caccattaat
     4861 gtgtgcgtca ttatggccaa aatttgattt atttaaaaaa tatgcaaata gcgaactgat
     4921 tttaacaaaa tcagcaatta atcaaactca aaagatagaa gtagacacaa tatatgtagg
     4981 gcatttagaa gatattgaat gccgacagac tcgcaatatc acacgttata caatggcttt
     5041 aacattaact aaaaatgatc aacatgtcat aacggttaca caaactttta ttaaggcgat
     5101 gaagtagaga tgaagtttaa tgagatatgg ataaatgaat atttggcgct cgtaaatgat
     5161 gataatccaa tacataatga gattgtgcca ggacaattag tgagtcaaat gatgctgatg
     5221 gctatgtcat tagagacaaa ccagtgtcaa attaactacg ttaaacctat tttaataaat
     5281 gaaaatatcg aattcattga acaacacgaa cacgaaatta tagcaattaa tgacgatgga
     5341 gagattaaaa taaaaatttc tttgagcaca aaaaaataac cgatattagc tgcatgaacg
     5401 catattaatt aggagatgaa aggacagcta atatcagtta tgtattgtta ttattattgg
     5461 gaacagagat gaatataggt tacgtttctt tctttgcacg gggatgcatt aatctaaaat
     5521 aataataaca actatatcaa tgtttaataa attctggatt attggaacga ttagtcaatt
     5581 taactaactt tcatatgatc tatatcgtct tgtaataaag agagcaattt gaatatttca
     5641 gtatcactaa atgaatcgtc acatttaatt gaaacatgct gaaacgtttt ggttataatt
     5701 tcataaactg gtgcgccttc atggtgatac tgtcgataaa taatcataac ctatattacc
     5761 tcctttgcta ctctatggtt atattataaa taacattttt atgtgtgaca tcaaccttaa
     5821 gtatcaactt tttatcagac atagaacgta tgatttacta agactattta tgtataaaag
     5881 ttctaaataa atatatattt atagagtcgc ctggcagtca tttgggaaat ataacatata
     5941 tgattagaga ggcatctatc gcaaaagaat gataatgata gaggtattga gcatatagat
     6001 gagtttaagt tcatcttgaa aataaagggt tatttagtca tagatgtaga tgtataggaa
     6061 atatttgtat gtattgttcg atatgtatga aattttcaat aaaagctaat aacgcttata
     6121 tgtaactttc aaatttaaat tatatacaga gcatgatgat tataaaaaaa taaccacatc
     6181 acataaattg agttcatacc caatttaagt ggtgtggcta ataatgttga tttatagatg
     6241 aaccgcctaa tcgttaaacc tctgttactt caacatcgat atgttcaata cggttgtatg
     6301 caccgtgatc cacaggacca acaaaatcat tcattttcca accgttttta atagcagaag
     6361 cgacgaaagc tttcgcgcta atcacagctt ctttcggtga cttaccgtta gctaaatatg
     6421 cagttgttgc cgcagcaaat gtacaaccag caccatggtt ataactttgt tggaacatgt
     6481 ctgttgttag ttgataaaat gtttgaccat catagtataa gtcatacgat ttatcttgat
     6541 ctaaagcttt gccaccttta atgatgacat gctgtgcgcc tttatcaaag ataattgttg
     6601 cagccttttt catatcttca attgaattta atttacctaa tcctgataat tgacccgctt
     6661 caaataagtt tggtgtcact accgttgctt taggtagtaa atatttaatc atcgcctcag
     6721 tatttccagg attaagcact tcatcttcgc ctttacaaac catgacagga tctactacaa
     6781 aatattgtgc attagatgcc tcatatactt ctccagcacg tttgattatc tcctcagtac
     6841 ctaacatacc tgttttaata gcatcaggtc cgattgataa agccgtttca agttgctttt
     6901 caaatacgtc cataggtaat ggtgtgacat cgtgtgacca tgtatcttta tccatagtaa
     6961 cgatggcagt taaagcgacc atgccatacg tatctaattc ttggaacgtt ttcaaatctg
     7021 cttgcatacc tgcgccagca cttgtgtcag aaccggcaat tgttaaaact ttctttaaag
     7081 ccattgagct tcactcctac ataataatat tgtattcatc atatcatttt taacctaatt
     7141 gaaaaatatt aagcattcaa tatttgatga ttgttgaaat gaatcattca tactattgta
     7201 acttttgaaa atgtcattca ctttagataa gtgtgatatg ttaaaatatg tcctgaggtg
     7261 agattgaatg gaatggtcgc aaatttttca tgacataaca acgaaacatg actttaaagc
     7321 tatgcatgat tttttagaaa aagaatattc gactgcaatc gtataccctg atagggaaaa
     7381 tatatatcaa gcgtttgatt taacaccgtt tgaaaatatc aaagttgtta tattaggaca
     7441 agacccgtat catggtccaa accaagcaca tggattagca ttttcagtgc aacctaacgc
     7501 aaaattccct ccatctttac gtaatatgta taaagaatta gcagatgata ttggatgcgt
     7561 tagacaaaca ccgcatttac aagattgggc aagagaaggc gtcttgttat tgaatacagt
     7621 tttaaccgta agacagggtg aagcaaattc tcatcgtgat attggttggg aaacatttac
     7681 tgatgaaatt attaaagcag tgtctgatta taaagaacat gttgtcttta ttttgtgggg
     7741 gaaacctgca cagcaaaaaa taaagcttat cgatacatct aaacattgta ttataaaatc
     7801 agtgcatcct agtccactgt ctgcatatag aggattcttt ggatcaaaac cgtattccaa
     7861 agcgaatgcc tatttagagt cagtaggaaa attaccaatt aattggtgtg aaagtgaggc
     7921 gtagatgttg aatagagaaa ctttaatagc acgaattgag caagaattag tacaagcaga
     7981 gcaggcacag catgaccatg actttgaaaa acatatgtat gccatacata tattaacatc
     8041 tttatatgct tcaacatcaa atacaccaca tattggtgaa caacaaatga atcgtcgtat
     8101 tgctaaccat aatcaaatgc cacaatcaca aataacgcag ccaactcatc aagtgacagc
     8161 tgctgaaatt gaagcgatgg gtggtaaagt aaatacgcat tcagcacatc atcataataa
     8221 gtcatattca caaccttcaa accaacaaca aagattagcg acagatgatg acattggcaa
     8281 tggtgaatcc atatttgatt tttaaaaagc aacaatgaaa cataattact taatagcttg
     8341 ttaagtatgt aggttaataa tcaagacgca tatactttta ttcgagtgtt cggatttaaa
     8401 catttattaa tactgaatta tataaggaga ggtagcaatg aaattattta ttattttagg
     8461 tgcattaaac gcgatgatgg ctgtcggaac aggtgcattt ggtgcgcatg gtttacaagg
     8521 aaaaataagt gatcactatt tatcagtatg ggaaaaagca acgacgtatc aaatgtacca
     8581 tggcttagca ttattaatta taggtgtaat tagtggtaca acttcaatca atgttaactg
     8641 ggctggctgg ttaatatttg ctggtattat tttctttagt ggatcattat atattttagt
     8701 attaactcaa attaaagttt taggtgcgat tacgccaatt ggtggcgtat tgttcatcat
     8761 tggatggata atgttaatca ttgcgacatt caaatttgct ggttaaattt taaaacttta
     8821 gattacctat gtaactaaac attaaatttt taataaaaat aatcaagaaa aagagttaca
     8881 aactcatctt ttgggtatag aataccttcg aggtgagttt ttatttatgg aaaaaaagaa
     8941 taagcaaata gatagaggcg atttaaaaca aaacctatct gaaaagtttg tatgggcgat
     9001 tgcatatggt tcatgtatcg gatggggcgc attcatctta ccaggagact ggattaagca
     9061 gtcaggtccg attgcagcat caattggtat agttattggt acattattaa tgatattaat
     9121 tgcggttagt tatggcgcat tagtagagag atttccagta tcagggggcg cgtttgcctt
     9181 tagtttctta agtttcggca gatatgtgag tttcttctca tcatggtttt taacttttgg
     9241 ttatgtctgt gtcgttgctt taaatgcgac cgcattcagt ttactagtta aattcttatt
     9301 gccagatgtc ttaaataatg ggaaactata caccattgcg ggctgggacg tttatattac
     9361 ggaaatcatt attgcgaccg tattactact tgtattcatg ctagtaacga ttcgtggcgc
     9421 aagtgtatct ggatcattac aatattattt ctgtgtggcg atggtaatcg tcgtattatt
     9481 gatgttcttt ggttcattct ttggtaataa ttttgcactt gaaaatttac aaccgttagc
     9541 tgaacctagc aaaggatggt tagtgtctat tgtggttatt gtatccgtgg caccatgggc
     9601 atatgttgga tttgataata ttccacaaac agcagaagag tttaactttg caccaaacaa
     9661 gacatttaag cttatcgtgt acagtttatt agcagcatca ttaacttatg ttgtcatgat
     9721 tttatacact ggttggttat caacaagtca tcaaagttta aatgggcagt tgtggttaac
     9781 aggtgctgtt acacaaacag catttggtta tattggatta ggtgtattag caattgcaat
     9841 tatgatgggt atatttactg gtttaaatgg attcttgatg agttcaagtc gcttgttatt
     9901 ttctatggga cgttcaggta ttatgccaac aatgtttagt aaattacata gtaaatacaa
     9961 aacaccatat gtcgcaatca tattcctagt aggtgtgtcg ttaattgcac cttggctagg
    10021 aagaactgca ttgacttgga ttgtagatat gtcatctaca ggtgtatcca ttgcctactt
    10081 tattacatgt ttgtctgcag cgaaattatt cagttataac aaacaaagta atacgtatgc
    10141 accggtttac aaaacgtttg ctattatcgg ctcatttgta tcattcattt tcttagcgtt
    10201 gttattagtg ccaggttctc ctgcagcact gactgcaccg tcttatattg cattacttgg
    10261 atggttaatc atcggtttaa tattctttgt gattcgatat cctaaattga aaaatatgga
    10321 taatgatgaa ttaagtcgct tgattttaaa tagaagtgaa aatgaagttg atgatatgat
    10381 tgaagaacct gaaaaagaaa aaactaaata ataaaagaat cgcacaatat accttcttca
    10441 ttcggaggcg tatcgtgcga ttttttgtat tataaattga catttaagac gaggcagctg
    10501 aaccttatat ataattgcta atagtttggg ctgagccaat tctaacaata tttataatcg
    10561 tttaaaagat ttcacgaacc cagaaacaat taatttagaa atttggtcgg cgaataataa
    10621 acctaatgcg atggctcctg caataagtgt tacttctaac attgtattta ttgctgtgct
    10681 gaaatttaat aagactaaat tttttgtagc atcgtatgct aagccaccag gtactaatgg
    10741 aatgataccc gttaccataa aaatgatggc aggttctttt tgtttacgag ccatataatg
    10801 acttaacaag cctaatgcta aactaccaaa gaaactagag tatatagtgt gcacattaaa
    10861 gccgttgaag aataaggtgt aaaccatcca tccacatgta ccaacgaaac cacatgatag
    10921 atataatttt ctaggtgcat caaaaatgac gcagaagaac attgaagcta aaaagctaaa
    10981 gataaagttt aagatccaaa acatagtctg atactcctat actaaaatta atacgctacc
    11041 aacgccagca ccgatgccaa acgcagtaac caatgcttct aatgatttcg ttgtgaacat
    11101 caacatgtgt ccaccaaata aatcttgtat tgcgtttgtt attaatacac caggaacaat
    11161 aggcatgact gctgcaatga taatagttgc caagtcacct gttgggataa gtgtatgtcc
    11221 aataacggcg ataatcccaa taactaatga accaatgaat tctgggataa actgtgcatg
    11281 taacttgcga tctaaaatct cagtgactag gtatcctaga ctacctgcta atatcgcagt
    11341 taaaacatca atcaatctac caccttgtaa atataagaaa ctcattgcaa tcattgctgc
    11401 agcaaaacct ttaaagggaa gactgctgtc acgcttagca acatatattt tttcaagttg
    11461 cgtttttgct tcggctaaag aaatttcatt gtttgtaatt tgacgcgaaa ttttattagc
    11521 ttgcgaaatt tttattaagt ttgtatctcg agaggtaatt ctaaatattc taggaaacga
    11581 ttccgaatgt aacgtaaact ggatgacagt gtttgtaaca aagctgttac tttcactgta
    11641 accaagtttt tttgcaatac gtgtcatggt atcttctaca cgcgtacctt ctgcaccaga
    11701 ttctaatagt atgcgagcag caagcatgac aacgtctttg ataagtacct cttgtttgta
    11761 ttcttctgaa tttatgtcca tttcatcacc attgtttata agaatttaat actcattata
    11821 gtttatacac tataaaataa ccacatgagc tttttgataa gttgttattg attttaaaaa
    11881 actaatattt gatactattg taataataat catcggaatt tttagtagtt aaaattctct
    11941 tgaagtataa aaaggaaaaa ttattgaaaa aaat
//
LOCUS       DAMZLE010000079        11836 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMZLE010000079 DAMZLE010000000
VERSION     DAMZLE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.079"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..141
                     /locus_tag="POB92_001802"
     CDS             <1..141
                     /locus_tag="POB92_001802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579287.1"
                     /translation="SWGEGPNTEADEKSAYNNVQVGDGPQQREIGFPISTDNASWGGT
                     TK"
     gene            476..3181
                     /gene="acnA"
                     /locus_tag="POB92_001803"
     CDS             476..3181
                     /gene="acnA"
                     /locus_tag="POB92_001803"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDI6579288.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3361..3828
                     /locus_tag="POB92_001804"
     CDS             3361..3828
                     /locus_tag="POB92_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6579289.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDDIDSL"
     gene            complement(4035..4331)
                     /locus_tag="POB92_001805"
     CDS             complement(4035..4331)
                     /locus_tag="POB92_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDI6579290.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4706..5314)
                     /gene="plsY"
                     /locus_tag="POB92_001806"
     CDS             complement(4706..5314)
                     /gene="plsY"
                     /locus_tag="POB92_001806"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI6579291.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5520..7511
                     /gene="parE"
                     /locus_tag="POB92_001807"
     CDS             5520..7511
                     /gene="parE"
                     /locus_tag="POB92_001807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI6579292.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQADRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7511..9913
                     /gene="parC"
                     /locus_tag="POB92_001808"
     CDS             7511..9913
                     /gene="parC"
                     /locus_tag="POB92_001808"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI6579293.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDFSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVVTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10163..11623
                     /locus_tag="POB92_001809"
     CDS             10163..11623
                     /locus_tag="POB92_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDI6579294.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4137 a         1714 c         2264 g         3721 t
ORIGIN      
        1 agttggggtg agggccccaa cacagaagct gacgaaaagt cagcttacaa taatgtgcaa
       61 gttggggatg ggccccaaca aagagaaatt ggattcccaa tttctacaga caatgcaagt
      121 tggggtggga cgacgaaata aattttgcga aaatatcatt tctgtcccac tcccatcaaa
      181 agaatgacat tgaaaaaaat attaaagttt ggtgccataa ttttctttct gctcaatgaa
      241 ataatacgtt taaaaattga attgaatcat tcgcgaaatt gataattatt ctcaattaat
      301 aaaaaatact tattacacct tacctgtatg ataagttttg cttatatact ctgattaaaa
      361 agtcaaaacc taaattaagc gttttctaag catgatgagt tgtttatcaa taaatataat
      421 aatacaattt aatcaaggca tataaatata aaaatgtatc aagggggatc attaaatggc
      481 tgcaaatttt aaagagcaat caaaaaaaca ttttgacttg aatggccaaa gttatactta
      541 ctatgattta aaagctgtag aagagcaagg tattactaaa gtttccaatt taccttattc
      601 aattcgtgtt ttgttagaat ctttacttcg tcaagaagat gattttgtaa ttacagacga
      661 tcatattaaa gctttaagtc agtttggaaa agatggaaat gaaggcgagg taccatttaa
      721 accttctcgt gttattttac aagatttcac aggtgtacca gccgtagttg atttagcttc
      781 tttacgtaaa gcaatggatg acgttggggg agatattact aaaattaatc cagaagtacc
      841 ggtggattta gttattgacc actcagttca agtggatagc tatgcaaatc cagaagctct
      901 tgaacgtaat atgaaattag aatttgaacg taactatgaa cgttatcagt ttttaaattg
      961 ggcaacgaaa gcatttgata attacaatgc agttcctcct gcaactggaa tagttcacca
     1021 agttaactta gaatatttag caagtgttgt acatgttcgt gatgtagatg gtgaaaaaac
     1081 tgcatttcca gatacattag ttggtactga ttcacataca acaatgataa atggtattgg
     1141 cgtactagga tggggtgttg gtggtattga agctgaagct ggaatgcttg gacaaccttc
     1201 ttatttccca attccagagg ttattggtgt acgactagta aattcattac cacaaggcgc
     1261 aacagcaact gatttagcgt taagagtaac tcaagagcta cgtaaaaaag gtgttgttgg
     1321 taaatttgtg gagttctttg gtccaggtgt acaacattta ccactagcag accgtgctac
     1381 aattgcaaac atggcaccag agtatggagc aacttgcgga ttcttcccag ttgatgatga
     1441 atctcttaaa tatatgaagt taactggtag atcagacgaa catatcgcgc tagtaaaaga
     1501 atatttgaaa caaaaccata tgttctttga tgttgagaaa gaagatccta attatacaga
     1561 tgttatcgaa ttggatttat caacagttga agcatcgctt tcaggaccaa aacgtcctca
     1621 agatttaatt ttcttaagtg atatgaaatc atcatttgaa aattctgtaa cagctccagc
     1681 aggcaaccaa ggacacggtt tagataaaag tgaatttgat aagaaagctg aaattaactt
     1741 taaagatgga tcaaaagcta caatgaaaac aggtgatatt gcaatagcag caattacatc
     1801 atgtacaaat acatctaacc cttatgtaat gttaggtgca ggtttagttg ctaaaaaagc
     1861 agttgaaaaa ggcttgaaag ttcctgaata cgttaaaact tctctagcac caggatcaaa
     1921 agttgttacc ggatatttaa gagatgctgg cttacaacct tatttagatg atttaggctt
     1981 caacttggtt ggttatggat gtacaacttg tatcggtaat tcaggtcctt tattaccaga
     2041 aattgaaaaa gcgattgctg atgaggacct attagtgaca tctgtattat ctggtaaccg
     2101 taactttgaa ggtcgtatcc atcctcttgt taaagccaat tacctagctt caccacagtt
     2161 agttgttgct tatgcattag ctggaacggt tgatattgat ttacagaatg aacctattgg
     2221 taaaggtaat gacggtgaag atgtatattt gaaagatatt tggccatcaa ttaaagaagt
     2281 ttcagatacc gttgatagtg ttgtaacacc tgaattattt attgaagaat ataataacgt
     2341 atacaataac aacgaattat ggaatgagat tgatgtaact gatcaacctc tatatgactt
     2401 tgatcctaat tcaacataca ttcaaaatcc atcattcttc caaggattat ctaaagaacc
     2461 gggtacgatt gttccattaa atggtttacg tgttatgggt aaattcggtg attctgtgac
     2521 aactgaccac atctctccag caggtgcaat tggtaaagat acgccagctg gtaaatattt
     2581 acaagatcat caagtgccta ttcgtgaatt taattcatat ggttcaagac gtggtaatca
     2641 cgaagtaatg gttcgaggta cgtttgctaa tatacgtatt aaaaaccaat tagcgccagg
     2701 tactgaaggt ggttttacaa cttattggcc aacaaatgaa gtaatgccta tctttgatgc
     2761 tgcaatgaaa tataaagaag atggtacagg tttagttgta ttagctggta acgattatgg
     2821 tatgggttca tctcgtgact gggcagcaaa aggtacaaac ttattaggtg ttaaaacagt
     2881 tattgcacaa agttatgaac gtatccatcg ttcaaattta gttatgatgg gtgtattacc
     2941 attagagttt aaaaaaggtg aatcagctga ttctcttggt ctagatggta cagaagaaat
     3001 ttctgttaat attgatgaaa atgttcaacc acatgactac gtcaaagtta ctgctaagaa
     3061 gcaagatggt gatttggtag aatttgacgc tatggttcgt tttgactcac ttgttgaaat
     3121 ggattactat cgtcacggtg gaattttaca aatggtttta agaaataaat tagcgcaata
     3181 aaaaatagat atcacagtaa aattttaatc ggtatttgag gcactttgat agtgctcaaa
     3241 aatacttcta taaactttat gatataatag gtcggttcaa aacctttgtg ttcttggccg
     3301 gcctcttctt gtattttatg ttagaatgag atataaagtt aaactgaaag aaggcttaaa
     3361 atgatatata gtattacaga aatagaagcg cgttatgctg aaactgataa gatgggtgta
     3421 atttatcacg ggaattatgc aacttggttt gaagttgcgc ggttggatta tatatcgaag
     3481 ttaggtttta gttatgctga tatggaaaaa caaggaatca tttcacctgt gactgacctc
     3541 aatgtcaatt ataaaaagtc tattttttat ccagaaaaag ttaaagttaa aacttgggtt
     3601 gaaaaatatt cgagattacg ttcagtgtat aaatatgaga tttttaatga aaaaggtgaa
     3661 cttgcaacta caggttccac agaattgatt tgcattaaag aagatacttt taagcctata
     3721 cggttggatc gttatttccc agattggcat gaagcttata gtaaagtgca aacgctcaat
     3781 aatgaaggga aaacagtaga gataatggat gatattgatt ctttataagt tactgtaaat
     3841 gtttggagag gcaatattaa caattaatct ataaaaaata gccaatacgt taacattaat
     3901 gtaattagcg tgaagataag ctaatatata gcaaaaacga acttgttcat acaacatcag
     3961 atgtgctaaa ccaaagtaca acttccgagg ttcaggacac atcagaatgt gtaaacaagt
     4021 tcgttttatt ttagttattt tgtggaataa gaaatttcat cttcgtgatc aactacattt
     4081 acaataatgt ggtcatcttc aaagtaccac aaatcttttt ccgctacgac aacatttaaa
     4141 tcgtcatatt gttgttcata gccaatatca acatcttcct ttggttcaac tgtaaaagca
     4201 ggactaaatc cttgcttgag ttggaattcg ccaccatatc ttacaaaaaa cacgagcact
     4261 ttattatttt caggcaactc aagttcattt ttaaaccaag ttactgctgc atcagtaagt
     4321 tctatttgca taacaatgac ctcctatatt tattacagaa tgggagtggg ccaacttgta
     4381 ttatttgtgg aatttcttat cgaaattctc tgtgttggag cccaccccaa cctgctcatt
     4441 attgtaagct gaatttccat aagcttctat gttgggaccc ccgaccagaa ttgaaaaaac
     4501 ctagttataa gcgcattttg ttcggttaac tacagctaat ataacattgt ggagcctatg
     4561 acattgattt atgtctcggt ctcaaatgtt cttgtaaaaa actaagtata gttgaatttt
     4621 aagttaaaaa aattgttttt gcaaaaataa aataatccat caactgcaaa aaaatatgca
     4681 atgatggact aatgtcaaaa aattgttaca tccattttat tttaggttct tcgcctctaa
     4741 aaatccttgc gatattagag cgatgtctaa ttatcaatat gattgaaact aagaaactaa
     4801 cgactaataa aatatagtct tgaatgataa gcgagccaat cacacagcaa attgctgcaa
     4861 cgatacttgc taaagaaaca tatttaaaaa tcttcaatac aataaagaag ataattgcaa
     4921 gtattagtaa aagtatcgga ttgactccca agacgacacc tgcactagtt gcaacagctt
     4981 tgccaccttg gaattttaaa taaacaggat aaacgtgtcc aagtatagcg aataagccaa
     5041 caattaaacc atttgtaaaa aaagtactaa tagggccatc tgcgtgaact ggtaaccata
     5101 aagggaagaa aacagttatg aaccctttga aaatatctag aaatgttacc aagaatcctg
     5161 caggacgacc taatactcta aagctattag tagcgccagt attaccacta ccaaattgtc
     5221 taatatcttt tttgaaaaat aattttccaa ttacgaatcc acttgggaaa gcgccgataa
     5281 gataacttag tagtaacatg acgattatca tcataaatat tacacatcct ttaatatctt
     5341 aggactattt tatcatattc gtttaaatta cggctaaaaa ttttaaaaac ggggattaat
     5401 atatggaatt aagctatgaa agttaattga tacttgcatt ttacgctgat ttatataaga
     5461 ataactattg tatagtttta aaaacgaacg tacgtttgca ggaggcgaaa tcattggcaa
     5521 tgaataaaca aaataattat tcagatgatt caatacaggt tttagagggg ttagaagcag
     5581 ttcgtaaaag acctggtatg tatattggat caactgataa acggggatta catcatctag
     5641 tatatgaaat tgtcgataac tccgtcgatg aagtattgaa tggttacggt aacgaaatag
     5701 atgtaacaat taataaagat ggtagtattt ctatagaaga taatggacgt ggtatgccaa
     5761 caggtataca taaatcaggt aaaccgacag tcgaagttat ctttactgtt ttacatgcag
     5821 gaggtaaatt tggacaaggc ggctataaaa cttcaggtgg tcttcacggt gttggtgctt
     5881 cagttgtaaa tgcattgagt gaatggcttg aagttgaaat ccatcgagat ggtaatatat
     5941 atcatcaaag ttttaaaaac ggtggttcgc catcttctgg tttagtgaaa aaaggtaaaa
     6001 ctaagaaaac aggtaccaaa gtaacattta aacctgatga cacaattttt aaagcatcta
     6061 catcatttaa ttttgatgtt ttaagcgaac gactacaaga gtctgcgttc ttattgaaaa
     6121 atttaaaaat aacgcttaat gatttacgca gtggtaaaga gcgtcaagag cattaccatt
     6181 atgaagaagg aatcaaagag tttgttagtt atgtcaatga aggaaaagaa gttttgcatg
     6241 acgtggctac attttcaggt gaagcaaatg gtatagaggt agacgtagct ttccaatata
     6301 atgatcaata ttcagaaagt attttaagtt ttgtaaataa tgtacgtact aaagatggtg
     6361 gtacacatga agttggtttt aaaacagcaa tgacacgtgt atttaatgat tatgcacgtc
     6421 gtattaatga acttaaaaca aaagataaaa acttagatgg taatgatatt cgtgaaggtt
     6481 taacagctgt tgtgtctgta cgtattccag aagaattatt acaatttgaa ggacaaacga
     6541 aatctaaatt gggtacttct gaagctagaa gtgctgttga ttcagttgtt gcagacaaat
     6601 tgccattcta tttagaagaa aaaggacaat tgtctaaatc acttgtgaaa aaagcgatta
     6661 aagcacaaca agcaagggaa gctgcacgta aagctcgtga agatgctcgt tcaggtaaga
     6721 aaaacaagcg taaagacact ttgctatctg gtaaattaac acctgcacaa agtaaaaaca
     6781 ctgaaaaaaa tgaattgtat ttagtcgaag gtgattctgc gggaggttca gcaaaacttg
     6841 gacgagaccg caaattccaa gcgatattac cattacgtgg taaggtaatt aatacagaga
     6901 aagcacgtct agaagatatt tttaaaaatg aagaaattaa tacaattatc cacacaatcg
     6961 gggcaggcgt tggtactgac tttaaaattg aagatagtaa ttataatcgt gtaattatta
     7021 tgactgatgc tgatactgat ggtgcgcata ttcaagtgct attgttaaca ttcttcttca
     7081 aatatatgaa accgcttgtt caagcagatc gtgtatttat tgctttacct ccactttata
     7141 aattggaaaa aggtaaaggc aaaacaaagc gagttgaata cgcttggaca gacgaagagc
     7201 ttaataaatt acaaaaagaa cttggtaaag gcttcacgtt acaacgttac aaaggtttgg
     7261 gtgagatgaa ccctgaacaa ttatgggaaa cgacgatgaa cccagaaaca cgaactttaa
     7321 ttcgtgtaca agttgaagat gaagtgcgtt catctaaacg tgtaacaaca ttaatgggtg
     7381 acaaagtaca acctagacgt gaatggattg aaaagcatgt tgagtttggt atgcaagagg
     7441 accaaagtat tttagataat tctgaagtac aagtgcttga aaatgatcaa tttgatgagg
     7501 aggaaatcta gtgagtgaaa taattcaaga tttatcactt gaagatgttt taggtgatcg
     7561 ctttggaaga tatagtaaat atattattca agagcgtgca ttgccagatg ttcgtgatgg
     7621 tttaaaacca gtacaacgtc gtattttata tgcaatgtat tcaagtggta atacacacga
     7681 taaaaatttc cgtaaaagtg cgaaaacagt cggtgatgtt attggtcaat atcatccaca
     7741 tggagacttc tcagtgtacg aagcaatggt ccgtttaagt caagactgga agttacgaca
     7801 tgtcttaata gaaatgcatg gtaataatgg tagtatcgat aatgatccgc cagcggcaat
     7861 gcgttacact gaagctaagt taagcttact agctgaagag ttattacgtg atattaataa
     7921 agagacagtt tctttcattc caaactatga tgatacgaca ctcgaaccaa tggtattgcc
     7981 atcaagattt cctaacttac tagtgaatgg ttctacaggt atatctgcag gttacgcgac
     8041 agatatacca ccacataatt tagctgaagt gattcaagca acacttaaat atattgataa
     8101 tccggatatt acagtcaatc aattaatgaa atatattaaa ggtcctgatt ttccaactgg
     8161 tggtattatt caaggtattg atggtattaa aaaagcttat gaatcaggta aaggtagaat
     8221 tatagttcgt tctaaagttg aagaagaaac tttacgcaat ggacgtaaac agttaattat
     8281 tactgaaatt ccatatgaag tgaacaaaag tagcttagta aaacgtatcg atgaattacg
     8341 tgctgacaaa aaagtcgatg gtatcgttga agtacgtgat gaaactgata gaactggttt
     8401 acgaatagca attgaattga aaaaagatgt gaacagtgaa tcaatcaaaa attatcttta
     8461 taaaaactct gatttacaga tttcatataa tttcaacatg gtcgctatta gtgatggtcg
     8521 tccaaaattg atgggtattc gtcaaattat agatagttat ttgaatcacc aaattgaggt
     8581 tgttgcaaat agaacgaagt ttgaattaga taatgcagaa aaacgtatgc atatcgttga
     8641 aggtttgatt aaagcgttgt caattttaga taaagtaatc gaattgattc gtagctctaa
     8701 aaacaagcgt gacgctaaag aaaaccttat cgaagtatac gagttcacag aagaacaggc
     8761 tgaagcaatt gtaatgttac agttatatcg tttaacaaac actgatatag ttgcgcttga
     8821 aggtgaacat aaagaacttg aagcattaat caaacaatta cgtcatattc ttgataacca
     8881 tgatgcatta ttgaatgtca taaaagaaga attgaatgaa attaaaaaga aattcaaatc
     8941 tgaacgactg tctttaattg aagcagaaat tgaagaaatt aaaattgaca aagaagttat
     9001 ggtgcctagt gaagaagtta ttttaagtat gacacgtcat ggatatatta aacgtacttc
     9061 tattcgtagc tttaatgcta gcggtgttga agatattggt ttaaaagatg gtgacagttt
     9121 acttaaacat caagaagtaa atacgcaaga taccgtacta gtatttacaa ataaaggtcg
     9181 ttatctattt ataccggttc ataaattagc agatattcgt tggaaagaat tgggacaaca
     9241 tgtatcacaa atagttccta tcgaagaaga tgaagtggtt attaatgtct ttaatgaaaa
     9301 ggactttaat acagatgcat tttatgtttt tgcgactcaa aatggcatga ttaagaaaag
     9361 tacagtgcct ctatttaaaa caacgcgttt taataaacct ttaattgcta ctaaagttaa
     9421 agaaaatgat gatttgatta gtgttatgcg ctttgaaaaa gatcaattaa ttaccgtcat
     9481 tactaataaa ggtatgtcat taacgtataa tacaagtgaa ctatcagata ccggattaag
     9541 ggcagctggt gttaaatcaa taaatcttaa agctgaagat ttcgttgttg tgacagaagg
     9601 tgtttctgaa aatgatacta tattgatggc cacacaacgc ggctcgttaa aacgtattag
     9661 ttttaaaatc ttacaagttg ctaaaagagc acaacgtgga ataactttat taaaagaatt
     9721 aaagaaaaat ccacatcgta ttgtagctgc acatgtagtg acaggtgaac atagtcaata
     9781 tacattatat tcaaaatcaa atgaagaaca tggtttaatt aatgatattc ataaatctga
     9841 acaatataca aatggctcat tcattgtaga tacagatgat tttggtgaag taatagacat
     9901 gtatattagc taaaaactat atgcaatcac gaaattaaat gataaaatac agtaatgtta
     9961 aattttgact aaattcaagg gatttatatt aaatgctgac caagtactta tcgttaaatt
    10021 agcgatatac tttgaccaaa ctaatataaa tctcttgtat tgtataagaa atgatgatgt
    10081 atctctatat ttactgaagt ttcaattcgg tctaatttta tagaaggtac atgattcatg
    10141 tagaggatgc gaggggttta ttttgaaaga tttcgatagt ttaattcctg gatggtttaa
    10201 agaatttgtc catgttggta ccgatttaat atggtctcaa tatttaattg gtctattatt
    10261 gacagctgga ttcttcttta caattagttc taaattcgtc caattacgaa tgttacctga
    10321 aatgtttaga gctttagtag aacgtccaga aactttagaa gatggtaaga agggtatttc
    10381 gccattccaa gcatttgcga ttagtgctgg ttcgagagtt ggtactggta atattgctgg
    10441 tgttgcgact gcgattgttt taggcggtcc aggtgcagtg ttttggatgt gggttattgc
    10501 atttataggt gcagcgagtg catttataga agcgactttg gctcaggttt ataaagtaca
    10561 tgataaagat ggtggattcc gtggtggtcc agcttactat attactaaag gtttaaatca
    10621 aaaatggcta ggtatcgtat ttgcgatttt aattacaatt acatttgcat ttgtatttaa
    10681 cacagtgcaa tctaatacaa ttgcggagtc gttaaatacg caatataata ttagtccagt
    10741 aatcacaggt attattttag caatcgtaac agctattatt atatttggtg gtgtacgtag
    10801 tattgctacg ttatcttcgt taattgtacc gattatggct atcatttaca ttggtatggt
    10861 tttagtaata ttgctattta atttagatca aattgttcct atgataggta cgattattaa
    10921 aagtgcattt ggtatcgaac aagtaactgg tggcgctgta ggtgctgcgg ttcttcaagg
    10981 tatcaaacgt ggtttattct ctaacgaagc tggtatgggt tctgcgccga atgcagcggc
    11041 aactgctgcc gtaccacacc ctgttaagca aggtttaatc caatcattag gtgtgttctt
    11101 tgatacaatg ttggtttgta cagcaactgc aatcatgatt ttactatatt caggactgaa
    11161 atttggtgat aacgcacctc aaggtgttgc agttactcaa tcagcactta atgagcattt
    11221 aggttctgct ggaggtattt tcttaacaat agcagttaca ctgtttgcat tttcatctgt
    11281 ggtaggtaat tactattacg gtcaatctaa tattgaattt ttatcaacaa accgtgtaat
    11341 attatttatc tttagatgtc ttgttgtagt acttgtcttt gtcggtgcag ttgtaaaaac
    11401 agaaacagta tggaatacgg cagacttatt tatgggctta atggcaattg taaacattat
    11461 ttccattata ggactgtcca atgtagcttt tgcattgatg aaagattatc aaaagcagaa
    11521 aaaagaaggc aagaaccctg tctttaaacc tgaaaactta gaaattaact tatttggaat
    11581 tagtgcttgg ggcgctaaca aatataagaa ctctgataaa taaatatttt taatgtaacg
    11641 attcattata taaaaatcaa ataaaccctg aggttttcaa attaaagcga aaatctcagg
    11701 gttttaaatt tgtactcaat aatcatctgt atcattacac tagttaatct aagatttgat
    11761 tatatattaa tatagtaagg ttgagattta tttcctccat tggtgtgcaa agactatacc
    11821 attgataaga catatt
//
LOCUS       DAMZLE010000080        11729 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMZLE010000080 DAMZLE010000000
VERSION     DAMZLE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.080"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            136..1428
                     /gene="obgE"
                     /locus_tag="POB92_001810"
     CDS             136..1428
                     /gene="obgE"
                     /locus_tag="POB92_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDI6579295.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1441..1896
                     /locus_tag="POB92_001811"
     CDS             1441..1896
                     /locus_tag="POB92_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6579296.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            1910..2512
                     /gene="ruvA"
                     /locus_tag="POB92_001812"
     CDS             1910..2512
                     /gene="ruvA"
                     /locus_tag="POB92_001812"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI6579297.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2544..3548
                     /gene="ruvB"
                     /locus_tag="POB92_001813"
     CDS             2544..3548
                     /gene="ruvB"
                     /locus_tag="POB92_001813"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI6579298.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            3550..4575
                     /gene="queA"
                     /locus_tag="POB92_001814"
     CDS             3550..4575
                     /gene="queA"
                     /locus_tag="POB92_001814"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI6579299.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4598..5737
                     /gene="tgt"
                     /locus_tag="POB92_001815"
     CDS             4598..5737
                     /gene="tgt"
                     /locus_tag="POB92_001815"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI6579300.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5756..6016
                     /gene="yajC"
                     /locus_tag="POB92_001816"
     CDS             5756..6016
                     /gene="yajC"
                     /locus_tag="POB92_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI6579301.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            6291..8570
                     /gene="secDF"
                     /locus_tag="POB92_001817"
     CDS             6291..8570
                     /gene="secDF"
                     /locus_tag="POB92_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDI6579302.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            8773..11046
                     /gene="recJ"
                     /locus_tag="POB92_001818"
     CDS             8773..11046
                     /gene="recJ"
                     /locus_tag="POB92_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI6579303.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     AIVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            11068..11586
                     /locus_tag="POB92_001819"
     CDS             11068..11586
                     /locus_tag="POB92_001819"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI6579304.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT         4213 a         1601 c         2287 g         3628 t
ORIGIN      
        1 aaatatcagc aatttgagta aagtaaattt aaaagcaact gcataagagc ccctaattat
       61 ttacaatata taaggggctc taatatgcta taattattgg gaaaatgaaa attatatgta
      121 aaagaggtga gatatatgtt tgtcgatcaa gtcaaaatat ctcttaaagc cggtgatggt
      181 ggtaatggta ttaccgcata tagaagagaa aaatatgtac catttggtgg accagctggc
      241 ggtgacggtg gtaaaggtgc ttcagtcgta tttgaagtgg atgaaggttt aagaacgtta
      301 ttagatttta gatatcaacg tcattttaaa gcaagcaaag gtgaaaatgg ccaaagtagt
      361 aatatgcatg gtaaaaatgc ggaagattta gtattaaaag ttccacctgg tacaattatt
      421 aaaaatgttg aaacagacga agtgttagca gatcttgttg aagatggtca aagagctgta
      481 gtagcgaagg gcggtcgagg tggccgaggt aattcacgtt ttgcaacacc tagaaaccct
      541 gcacctgact tcagtgaaaa aggtgaacca ggtgaggaat tagatgtatc tttagaattg
      601 aaattattag ctgatgtagg attagtaggt ttccctagtg tgggtaaatc gactttatta
      661 tctatcgttt caaaagctaa gcctaaaatt ggggcatatc attttacaac gattaaacca
      721 aatctaggtg ttgtttcaac gcctgatcaa cgtagttttg ttatggcaga tttaccaggt
      781 ttaattgaag gtgcatctga tggcgttgga ttaggacatc aatttttaag acatgtagag
      841 agaacaaaag ttattgttca catgattgat atgagcggtt ctgaaggtag agaacctatt
      901 gaagattata aagtcattaa tcaagaatta gctgcgtacg agcaacgttt agaagataga
      961 cctcaaatcg tagtagctaa caagatggat ttacctgaat cacaagataa tttaaacttg
     1021 tttaaagaag aaattggcga agatgtgcca gttattccag tttcaacaat aacgcgtgat
     1081 aatattgatc aattattata tgcaatagca gataaattag aagaatataa agatgttgac
     1141 ttcacagttg aagaagagga gtcagttggc attaaccgag tattatataa acatacaccg
     1201 tcacaagata aatttacaat ttcaagagat gatgatggtg cttatgtggt aagtggtaat
     1261 gctattgaaa gaatgtttaa aatgactgac tttaacagtg atccagcagt acgtcgattt
     1321 gctcgtcaaa tgcgttcgat gggtattgat gatgcactta gagaacgtgg ttgtaaaaat
     1381 ggtgatatcg ttagaattct tggcggagaa tttgaattcg ttgaatagga gcgtaacatg
     1441 atggacaata aagattataa aaagttttat ttaattagag aagatgtctt gcctgaatcc
     1501 gtggttaaaa cattgaagat taaagatgcc ttaaaaagtg atccgacatt gtccatttat
     1561 gatgccgtta aacagtttga tctatctaga agtgcttttt ataaatatag agaaacgata
     1621 tttccagtag acgataaaat gcttgaccat cgagaattta cattaatttt atatgtaact
     1681 gatattgtgg gtatgttggc acgtgtacta gatgttatat caaagttaga actatctgta
     1741 ttaacgattc atcaaagtat tccaatggaa gaaaaagcaa caataacatt atcactgaat
     1801 gctaaatcta aagaaacttc agtagaagat gttattggtg ctttgagaaa tttagattat
     1861 gtatcaaaag tagaattaat tagtatgagt atgtaaggat gtgcctataa tgtacgcgta
     1921 tgtcaaaggt aagttaacac atttatatcc tacacacgta gttgttgaaa ctgctggtgt
     1981 tggttatgaa attcaaacac caaattctta tcgttttcaa aagcatctag atcatgaagt
     2041 tttaattcgt acatctttaa ttgttcgtga agatgcacaa ttattgtatg gatttagtag
     2101 tgaagaagag aaagatatgt tcttgagttt aattaaagtt actggtattg gtccgaaatc
     2161 agctttagct attttagcga caagtacgcc taatgaagta aaacgtgcca ttgaaaatga
     2221 aaatgatacg tatttaacta aattcccagg aattggtaag aaaacggcaa gacagattgt
     2281 cttagattta aaaggtaaag tgaaaatcac tgaagaagat agcgattcat tattacaagt
     2341 agacgctact tcgacggtgc aagatcaatt cgtgcaagaa gcaatgttag cgttagaagc
     2401 attaggttat tctaaacgag agcttgcaaa agttgagaaa acgttaaata aaaataaata
     2461 tgactcagtt gatgaagctg ttaaggcagg tcttcaatta gttgtatctt aattttaaat
     2521 agattaatag gggaagtgtt gtcatgaatg agcgtatggt tgatcaatca atgcatagtg
     2581 aagaaactga tttcgaattg tcgcttagac ctacgagatt acgacaatat attggtcaaa
     2641 attcaataaa aagtaattta gaagtattta ttaaagcggc taaacttcgt catgaaccat
     2701 tagatcatgt attgcttttt ggcccccctg gattaggtaa gacaacatta tctaatatca
     2761 ttgccaatga aatggaagtt aatatacgta cagtatcagg gccttcatta gaaagacctg
     2821 gtgatttggc tgcaatttta tcaggacttc aacctggaga tgtattgttt attgatgaaa
     2881 tacacagact gagtagtgtt gttgaagaag tgttataccc tgcaatggaa gatttctttt
     2941 tagatattat cattggtaaa ggcgatgagg ctagaagtat ccgtatcgac ttacctccat
     3001 tcactttggt aggtgcaaca acgcgagctg gcagcttaac aggtccacta agggatcgat
     3061 ttggtgtgca cttaagatta gaatattata atgaatcaga tttaaaagaa atcattatta
     3121 gaacagctga ggttttaggc acaggtattg atgaagaaag tgccattgaa cttgctaaac
     3181 gttctagagg gactccaaga gtagcaaatc gactattgaa gcgggtaaga gacttccagc
     3241 aagtgaatga agatgaacaa atatacattg aaacaacgaa gcacgcatta ggtttacttc
     3301 aagttgatca acacggacta gattacattg atcataaaat gatgaactgt attattaagc
     3361 agtataatgg tggacctgtt ggtttagata cgattgccgt aacaattggt gaagaacgta
     3421 ttacaattga ggacgtttat gagccatttc ttattcagaa aggcttttta gaacgtacgc
     3481 cacgtggcag aaaagcaaca ccattagctt atgaacattt tgcaaagtcg aatgaggaga
     3541 gaggataacg tgaatattga agaatttgac tatgacttac cagaatcatt aattgctcaa
     3601 acgcctttaa aagatcgtga tcatagtcgt ttattagtca tggatagaga aactggtgaa
     3661 atgaaacatt tacatttcaa agatatcatt gagtatttta gacctggtga tacattagtg
     3721 cttaacgata cgcgagtaat gccagctaga ctttttggtt taaaagaaga aactggtgca
     3781 aaagttgaaa tgttaatgtt aactcaaatt gaaggtaatg attgggaagt cttactgaaa
     3841 ccagctaagc gtattaaagt tggtaataaa ttgaattttg gtaatggcaa aattatagct
     3901 gagtgcataa aagaaatgga tcaaggtgga cgcatcatgc gtttacatta tgaaggtatt
     3961 ttacaagaaa gattagatga attaggggaa atgccactgc caccatacat caaagaacgt
     4021 ttagatgatc cagatcgtta tcaaacagtt tacgctaaag aaagtggttc agcggcagca
     4081 ccaacagcag gattacattt tactgatgag ttattaactg aaattaaaaa taaaggcgtt
     4141 aatatcgcat ttgttacatt acatgttggg ttaggtacgt ttagaccggt gagcgtcgat
     4201 gatgtgaatg accacgaaat gcatagtgaa tattatcaaa tgacacaaga aacagctgat
     4261 ttattaaatg atactaagtc caaaggacat cgcattatat cagttggtac aacttcaaca
     4321 cgtacacttg aaacaattcg acgcgatcat gataaatttg ttgaaacgag tggctggact
     4381 aatatattta tttatccagg atttgatttt aaagcaattg atggccagat tactaatttt
     4441 catttaccaa aatcaacatt agttatgcta gtatcagcgt ttagtactcg tgaaaatgtt
     4501 ctgaatgctt ataaaacagc agtaaattta gaatatagat tctttagttt tggcgatgca
     4561 atgttaatta tataaaaaga atgtgaggat tttgaatatg cctgcagtaa catacgaaca
     4621 cattaaaact tgtaaacaat caggtgcgcg tttaggtatc gtgcacacac cacacggttc
     4681 atttgaaaca cctatgttta tgccagttgg tactaaagca accgttaaaa caatgagtcc
     4741 agaagagtta agacaaattg aagcaaaaat cattttgggc aacacatatc atttgtggtt
     4801 acaacccgga aatgatatta tcaaacacgc tgggggatta cataaattca tgaattggga
     4861 tggtccgatt cttacagatt caggcggttt ccaagtgttt agtttaagta atttacgtaa
     4921 aattacagaa gaaggcgtgg aatttagaca tcatactaat gggtctaaat tatttttgag
     4981 tcctgagaaa tcaatgcaaa ttcaaaatga tttaggatct gatattatga tggcatttga
     5041 tgaatgtcca ccgatgcctg ctgaatatga ttatgtaaaa aaatctattg aacgtacaac
     5101 acgttgggcg aaaagatgtc tagatgcaca ccaaagacct gaagatcaag cattgttcgg
     5161 cattatacaa ggtggcgaat atgaagattt aagagaacaa agtgcaaagg atttagtaga
     5221 attagatttt cctggttatg caatcggcgg tttgtcagtt ggtgaaccta aaccggttat
     5281 gtataaaatg gttgaacata cagagcagtt tatgcctaaa gataaaccaa gatatttaat
     5341 gggtgtagga tctccagatg cgttaatcga atgtagtatt cgcggcatgg atatgtttga
     5401 ttgtgtctta ccgacacgta ttgccagaaa tggtacttgt atgacatcgc aaggtcgttt
     5461 agttattaaa aatgcaaaat ttgcagatga tttaagaccg ttagatgaga attgtgactg
     5521 ttatacatgt caaaactatt caagagcgta tatacgtcat ttaatcaagg cagaggaaac
     5581 ttttggtatt cgtcttacta ctattcataa tttacatttt ctgctaaaat taatggaaga
     5641 tataagacaa gccattcgag aagatcgtct tttagatttc aaagaagaat tcttcgagca
     5701 atatggatta aatgttgaga acccaaaaaa cttttaagca agaggagcgt ataaaatgca
     5761 attttcatta ctaatatata tagtcgtaat ttttgcggtt atgtatttct tgatgatcag
     5821 accacaacaa aaacgtgcga aacagcatcg tgagttgatt aataacattc aatctggtca
     5881 aagaattaca actattggtg gtattaaagg tactgttaaa gcagtagatg aaacaactgt
     5941 tgttattaca gttaatggtc atggtactga attaactttc gaaaaacctg ctattaaaca
     6001 agttgaccct tcataatgag aagtggtatt aaaaaggatg aaacgaatag cgtttcatcc
     6061 tttttaagtt ataagtagta tgtagaatag cttgttttta ttccaaaaaa agaaattgtg
     6121 atacataata attgttaaat ttaaattaaa tatatctatg actattttta ctccctgctc
     6181 tagtatgtta atatgtataa ggctaataaa ttttgtaaga aagatttaac ttgcgttata
     6241 aaatagtatt caagtattct aaatacatac gtaaataatg aggtgttcat gtgaagaaaa
     6301 gtagtagaat aattgcgttc ttgcttttag tcgtattgtt gtttgccgga atggctgcaa
     6361 cttataaaag tgttataaag aatgtcaatt taggacttga tttgcaaggt ggatttgagg
     6421 tgctttatca agtcgatcct ttaaataaag gcgacaaaat agataaaaaa gccttacaat
     6481 caacagcgca aacattagaa aatcgtgtta acgttttagg tgtttcggaa cctaaaattc
     6541 aagttgaaga acctaataga attagagtac aacttgctgg tgtaacagac cagaatgaag
     6601 cacgtaaaat tttatcttct caagctaatt taacaattcg cgatgcagaa gataaagtaa
     6661 aattaagcgg ttctgatatt aaacagggat ctgccaaaca agaatttaaa caggaaacaa
     6721 accagcctac agttactttt aaagtaaaag acaaaaacaa atttaaaaaa gtaacggaag
     6781 aaatctctaa gaaacgtgat aatgtcatgg ttgtttggtt agatttcaaa aaaggtgaca
     6841 gctataaaaa agaagcacaa aagaaaaacc ctaaatttat ttctgcagca agtgttgatc
     6901 aaccaatcaa ttctgatagc gtagaaatct cgggtggatt taaaggacaa gaaggcgtta
     6961 aaaaagcaaa acaaatcgcc gaattgttaa atgcaggttc tttaccagtt gacttgaaag
     7021 aaatttattc aaactcagta ggtgcccaat ttggtcaaga tgcattagat aaaactgtgt
     7081 ttgcatcgtt tattggtgta gcattaattt atttattcat gcttggattc taccgattac
     7141 ctggtttagt agcgattatt gcattgacaa cttatatcta tctaacgtta gtagcattta
     7201 atttcatttc cggggttcta actttaccag gattagcggc gttggtatta ggtgtaggta
     7261 tggctgtaga tgccaatatt atcatgtatg agcgtattaa ggatgaactt cgaataggta
     7321 gaacgataaa gcaagccttt tctaaagcaa acaaaagttc attcctaaca atttttgatt
     7381 ctaacttaac aacagttatc gccgcagcag tattattctt cttcggtgaa agttcagtta
     7441 aaggtttcgc gacaatgtta ttattaggta ttctaatgat ctttgttaca gccgtgttct
     7501 tatcaagatt cttattatca ttacttgttt catcaaatat attcaaaaat caattttggt
     7561 tatttggtgt taaaaagaat aaacgacatg atattaatga aggtgtagat gttcatgacc
     7621 ttaaaacttc attcgagaaa tggaattttg ttaaattagc taagccatta attggagtaa
     7681 gtattttaat tgtagttgtc ggtttagtta ttctttatat cttcaagtta aacttaggaa
     7741 ttgatttctc aagtggtaca cgcgtagatt tccaatctaa acaagcaatt actcaacaaa
     7801 aggtagagca agttgtaaaa gatagtggac ttaaagcgga tcaaattcag atcaatggta
     7861 aagacaataa agtagcaact gttcaattta aagacgattt aacgcgtgct caagataata
     7921 aattgagtga taatattaaa tcgaaattcg gtgacacacc tcaaattaat actgtatcac
     7981 caattattgg acaggaatta gcaaaaaatg caatgcttgc attaatctat gcatcgatag
     8041 gtatcatcat ctatgtatca ttacgatttg aatggcgaat gggtctttca tctgtattgg
     8101 cattattaca tgatgtattc attatagtag cgattttcag tttatttaga attgaagtag
     8161 atttaacatt tatcgccgct gtattaacaa ttgtcggtta ttcaattaat gatacaatcg
     8221 taacgtttga ccgtgtacgt gaaaacttac aaaaggttaa agtgattacg acaacagaac
     8281 aaattgatga tatcgttaat agatcaatta gacagacaat gacacgttca attaatacag
     8341 tattaacagt tattgtagta gtagttgcta tactattctt cggtgctcct acgatattca
     8401 actttacttt agcattattt atcggattga tttctggtgt attctcttca atcttcattg
     8461 ccgttccgct atggggaata atgaaaaaac gtcagctgaa aaaatcgccg aaacacaaat
     8521 tagttgtata taaagaaaag aaatcgaacg atgaaaagat tttagtttaa aatgaattaa
     8581 gcggtatgtg aaacaataaa gagttcacat accgcttttt actttattta aatttaacaa
     8641 aatatatata aataagttcc cgcattcatt tttaaaaaat ggtataatta aagtcttgat
     8701 tatttagata agtgcttttt atttaaagta attttagata taatgtcttt tggaaatgag
     8761 gcgtaaatat atatgattaa accgaagtat aagtggaaat taactaaacc agctgaatac
     8821 ataagtgatg aattaacatc aaaattaaag ttaacgccaa tcgtaaaaaa aatattagaa
     8881 agtaaatcga ttattgatga acaagcaata gaatccatca tttcagatac agatatcaat
     8941 catgatgcat tgcaattgag cgatatgact aagactattg aaagaattaa aagagcgata
     9001 gcgaatgatg agaaaatcct agtttatggt gattacgatg ctgacggtgt tacctcaact
     9061 acaattttag tgacaacatt acaactttta ggtgcacaag taggatggca tattcctaac
     9121 cgctttaccg aaggatatgg accaaacgaa ctagcattta gaaatgctca tgatgaaggc
     9181 ataacattaa tcataactgt tgataatggc atacaaggtc ataatgaaat taaaatggtt
     9241 caagatttag gagttgatgt cattgtaact gatcatcatg aaattggtag tacattacca
     9301 gaagcatatg caatcgtaca tccaatgcat ccatcgttta attatccgtt ccaacaattg
     9361 tgtggtgcag gtgttgcata caaattagca caagcattga ttgaaaatgt acctgattat
     9421 tttaaagcaa tagttgcgat aggtaccatt gcggatttag tatccttaac tgatgaaaat
     9481 cgatcattag tgaaacaagg attaaaagtc ttaaatgatc aatgtccgac ctctgttaaa
     9541 gcattgctaa aagaagcggg atacaatgac aatattgatg aagaaacaat tggttttatt
     9601 attggaccta gacttaatgc tgtaggtcgt ttagatgacg catcattagc ttgcgaattg
     9661 ttgatgactg acgacgaaga agaagctgca tttttagcag aacaagttga acattttaac
     9721 cgtgaaagaa aagatattgt agcaactatc acagaagaag caatggctat ggcagaaacg
     9781 aaagttaaaa agggagattt atttttactt ttagcaaaag aaaattggca tgaaggtgtc
     9841 ttaggtattg ttgcttcgaa aattgtcgaa acatttgctt tgccaacatt aattttaaat
     9901 attgataggg agcaaaatca tgcaaaaggt tccgccagat caattgatca agtgtcgatg
     9961 tttgaaatac tatcagcaca tcaagagtta atcgctaaat ttggcggcca tcatatggct
    10021 gcaggtatga cgatggatat tgaaaatata gaatcgttag ctgaaggttt aaataaatgg
    10081 atgaaagaat tatctaaaac aacttcgtta gatcccgtaa agcaagtaga tgtattatta
    10141 acagaaaatg atataacgat taaaaatatt cgtgatatga atcgtttaag acccttcgga
    10201 acagattttt ctagacctat ttttgaaatg gatgatttat cagtttcttc agttaaggcg
    10261 ataggtcaac aaaaaaatca tctaaaattg acacttggtg aaagtaatat cgccgcgcta
    10321 ttttggcaaa atggacactt agaacctgag ttacaagatg aacagccaat taatatatta
    10381 ggatctgttc aaatcaacga atggaatggt aatcaatcac cgcaaataat tattcaagat
    10441 attgcgatga atgaacagca aatattagat tatagaagta agcgaaaaag tttacctttt
    10501 acagaaaatg atgaaaatat tgtcgtgctt attcatccta aaagtgataa agtaaatgcg
    10561 aatgaatatt attatggtga agaaattaaa caacaaactg ataaagtagt attaagagat
    10621 ttaccaacgt caatggaaga cttgtctaat tccttgcaac aactgcaatt ttctcaactt
    10681 tatatagttt tgcaacataa tcattcgatt tacttcgatg gtatacctaa tatggatatt
    10741 tttaaaaagt gttataaagc attaataact aaacaagaaa caaatatcca gaaagagggt
    10801 atgttattgt gtcaacattt aagtgtgaaa ccagatacac ttaaattcat gttgaaagtt
    10861 ttcttagact taaaatttgt aacacaagaa gatggtttaa ttcgaatcaa tcaacaacct
    10921 gataaaagat cgattgattc cagcaaagta tatcaattaa gacaacaacg tatggatgtt
    10981 gaaaagcaat tattatatca agatttttca gaaataaaaa attggataaa gtcacaattg
    11041 tcgtgagcaa tttaggagga aatattaatg gatttaaagc aatacgtatc agaagttcaa
    11101 gattggccga aaccaggtgt tagtttcaag gatattacta caattatgga taatggtgaa
    11161 gcatatggct atgcaacaga taaaattgta gaatacgcaa aagacagaga tgttgatatc
    11221 gttgtaggac ctgaagcgcg tggctttatc attggctgtc ctgtagctta ttcaatgggg
    11281 attggctttg cacctgttag aaaagaaggg aaattacctc gtgaagtcat tcgttatgag
    11341 tatgacctag aatatggtac aaatgtttta acaatgcaca aagatgcaat taaaccaggt
    11401 caacgtgtgt taattacaga tgatttatta gctactggtg gtacgattga agcagcaata
    11461 aaattagttg aaaaattagg cggtatcgta gtaggtattg catttataat tgaattgaaa
    11521 tatttaaatg gtattgaaaa aattaaagat tacgatgtta tgagtttaat ctcatacgac
    11581 gaataataaa taatataatt ttatcaaatg aaatccttca tcaaatgtat aagaaccaat
    11641 gacttaatta aaaaagttgt ttaagttttc ttaacatgag atgttaggat tttttattta
    11701 ctgaaaatgt tagatgattg agcattata
//
LOCUS       DAMZLE010000081        11534 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMZLE010000081 DAMZLE010000000
VERSION     DAMZLE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.081"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            16..666
                     /locus_tag="POB92_001820"
     CDS             16..666
                     /locus_tag="POB92_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6579305.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            663..899
                     /locus_tag="POB92_001821"
     CDS             663..899
                     /locus_tag="POB92_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDI6579306.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            1168..3153
                     /gene="uvrB"
                     /locus_tag="POB92_001822"
     CDS             1168..3153
                     /gene="uvrB"
                     /locus_tag="POB92_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDI6579307.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIEEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            3161..6007
                     /gene="uvrA"
                     /locus_tag="POB92_001823"
     CDS             3161..6007
                     /gene="uvrA"
                     /locus_tag="POB92_001823"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI6579308.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            6614..7546
                     /locus_tag="POB92_001824"
     CDS             6614..7546
                     /locus_tag="POB92_001824"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDI6579309.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            7552..8391
                     /locus_tag="POB92_001825"
     CDS             7552..8391
                     /locus_tag="POB92_001825"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI6579310.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYVENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEKLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            8399..8884
                     /locus_tag="POB92_001826"
     CDS             8399..8884
                     /locus_tag="POB92_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6579311.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            8892..10331
                     /locus_tag="POB92_001827"
     CDS             8892..10331
                     /locus_tag="POB92_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6579312.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            10398..11333
                     /gene="trxB"
                     /locus_tag="POB92_001828"
     CDS             10398..11333
                     /gene="trxB"
                     /locus_tag="POB92_001828"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI6579313.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         4154 a         1541 c         2288 g         3551 t
ORIGIN      
        1 gaggaggtaa ttgaaatggg tgtacatcaa tattttaaaa gattatcaga tatggaaaga
       61 cttataagat tacctggaaa atttaaatat tttgaacaca atgttgcagc acactccttt
      121 aaagtaacta aaattgctca atatctagca acagttgaag aatatcatgg acgaaagatt
      181 aattggaaaa gcttatatga aaaagcatta aatcatgatt tcgccgaagt gtttactggt
      241 gatataaaaa cacctgttaa atatgcgagt agtgaattaa aaaaattatt ttcgcaagtt
      301 gaagaagaaa tggtagagac ctttattgaa gaagaaattc cattacaata tagagatgtt
      361 tataagcaac gactgcaaga aggtaaagat gattcattag aaggccaaat actttcagtt
      421 gctgataaaa ttgatttgct ttatgaaaca tttggagaaa tacaaaaacg taatcccgaa
      481 gaattatttt tcgaaattta tgaaatgagt ctagaaacaa ttattcaatt tgaccattta
      541 gcatctgtac aagattttat taataatatc attccagaaa tgttgactga aaactttata
      601 cctagaacag aattaagaga aacaaccatg aacattttaa ataaaagaaa agaggaaaat
      661 gaatgatatg gtattttagc gcagcattct ttccatgtgt cttggtagta ttatttagtg
      721 taataacaag aagtaagtgg gtcggtacta ttctgacatt aattttaatt ggtgcctcaa
      781 tctataaaga gtatttccat aacgagtgga ttatttttat tgatgtagtg tcattattag
      841 ctggttattt aattatagat caactcgaat ttcataaaca tcaagatgaa gatcgctaag
      901 attaacttta aaataatgtt tcaaacaaat ttgttgaaac aaaatgatga ttaatataat
      961 gtgtatttac atactaaaaa taacaagata aacgatttga tttaaggcaa gcatagttag
     1021 cacaactatg tttgtttttc tttgttcgac atttttacga acaaacgttt gctttttgtg
     1081 tgactacttt gctaaaatat gtaatgagaa agcaaagagt tgagcggaaa tataaataat
     1141 acgttgaaag aggaggcata tgtgacaatg gttgaacatt atccttttaa aatacattct
     1201 gattttgagc ctcaaggtga ccaaccgcaa gcaattgaag aaatcgtgga aggtattaaa
     1261 gcggggaaaa gacatcaaac tttattaggt gctactggca cagggaaaac atttacgatg
     1321 agtaatgtta ttaaagaagt tgggaaacca acgttaatta tcgcgcataa caaaacatta
     1381 gcaggacaat tatatagtga gtttaaagaa ttttttcctg aaaacagggt ggaatacttt
     1441 gtaagttact atgattatta tcaaccagag gcatacgtac cgtctactga cacttttatt
     1501 gaaaaagatg cctcaatcaa tgatgaaatt gatcaactac gacattctgc tacaagtgca
     1561 ttatttgaac gcgatgatgt aattattatt gctagtgtaa gttgtatata tggtttaggt
     1621 aatcctgaag aatataaaga tttagtagta agtgttcgag ttggtatgga aatggataga
     1681 agtgaattac ttagaaaact tgtagatgtg caatatacac gaaatgacat cgatttccaa
     1741 cgaggaacgt ttcgagtgcg tggtgatgta gttgaaatat tcccagcctc taaagaagaa
     1801 ctttgtataa gggttgagtt tttcggcgat gagattgacc gtatccgaga agttaactac
     1861 ctaacaggtg aagtgttgaa agaaagagaa cattttgcga tattcccagc ttctcacttc
     1921 gtaacacgtg aagaaaagtt gaaagttgcg attgaacgta ttgaaaaaga attggaagaa
     1981 cgattgaaag aattacgaga tgagaataaa ttactagaag cgcaaaggtt agaacagcgt
     2041 accaactatg atttagaaat gatgcgagag atgggattct gttcaggaat tgaaaactat
     2101 tccgtacatt taactttgcg gccactgggt tcgacaccat atactttatt ggattacttt
     2161 ggcgatgatt ggttagtaat gattgatgaa tcacatgtga cattaccgca agttcgaggc
     2221 atgtataacg gagacagagc gcgtaaacaa gttttggtgg atcatggatt tagattaccg
     2281 agtgcattag ataaccgtcc acttaaattt gaagaatttg aagaaaagac aaaacaactt
     2341 gtgtatgtat ctgcaacgcc tggaccatac gaaattgaac atacggataa gatggttgaa
     2401 caaattattc gtcctactgg tttactggat cctaagattg aggttagacc tactgaaaat
     2461 caaattgacg atttattaag tgaaattcaa acaagagttg agcgtaatga acgcgtactt
     2521 gttacaacgc tcactaaaaa gatgagtgaa gatttaacca catacatgaa agaagcgggt
     2581 attaaagtta attatctgca ttcagaaatc aagacattag aacgaattga aataattaga
     2641 gacttacgaa tgggtacata tgatgttatc gtaggtatta atttattaag agagggtatt
     2701 gatataccag aagtttctct agttgtcata ttagatgcag ataaagaagg gtttttacgt
     2761 tctaaccgct cattaattca aacaataggt agagctgcgc gtaacgataa aggtgaagtc
     2821 attatgtatg ccgataaaat gactgattcg atgaagtatg caattgatga gacacaacgt
     2881 cgtcgagaaa tacagatgaa acataatgaa aaacatggta ttacacctaa aacaattaat
     2941 aaaaaaatac atgatttaat tagtgctact gttgaaaatg acgaaaataa tgacaaagca
     3001 caaactgtga tacctaagaa gatgacgaaa aaagaacgtc aaaagacaat cgacaatata
     3061 gaaaaagaaa tgaaacaagc agcgaaagat ttagatttcg agaaagctac agaattaaga
     3121 gatatgttat ttgaattaaa agcagaaggg tgacaagtaa atgaaagaac catccatagt
     3181 agtaaaaggt gctcgtgcgc ataacttgaa agatattgat atcgaactac ctaaaaataa
     3241 attaattgtt atgacaggtt tatctgggtc aggtaaatcg tcattagcat tcgatactat
     3301 atatgctgaa ggacaacgac gttatgttga atcattaagt gcctatgcgc gtcaattttt
     3361 aggccaaatg gacaaaccag atgttgatac aattgaagga ttatcgccag caatttcaat
     3421 agatcaaaaa acaacaagta aaaatccaag atcaactgta gcaacagtaa cagaaatata
     3481 tgattatata cgtttgttat atgcacgtgt tggtaaacct tactgtccaa atcacaatat
     3541 agaaattgaa tcgcaaacag tacaacaaat ggttgaccgc attatggaat tagaggcacg
     3601 tacaaagatt caattattag cacctgtcat cgctcatcgt aaaggtagtc atgaaaagct
     3661 aatcgaagat attggtaaaa aaggttatgt acgtttaaga atcgatggcg aaattgttga
     3721 tgtaaatgat gtacctactt tagataagaa caagaatcat acaatagaag ttgttgtaga
     3781 ccgattagtt gttaaagatg gaattgaaac acgactagct gactctatag aaactgcctt
     3841 agagctttca gaaggacaat taacagtcga tgtcattgac ggggaagacc ttaagttttc
     3901 agaaagccat gcttgtccta tatgtggatt ttcaatcgga gagttagaac caagaatgtt
     3961 tagctttaac agtccttttg gtgcttgtcc gacatgtgat ggcttaggcc aaaagttaac
     4021 agtcgatgta gacttggttg ttcccgacaa agataagacg ctaaacgaag gtgcaataga
     4081 accttggata ccgacgagtt ctgattttta tccaacattg ttaaaacgtg tttgtgaagt
     4141 ttataaaatc aatatggata aaccttttaa aaagttaaca gaacgtcaac gtgatatttt
     4201 attgtatggt tctggtgaca aagaaattga atttacattt acacaacgtc aaggtggtac
     4261 tagaaaacga acaatggttt tcgagggtgt agttcctaat ataagtagac gattccatga
     4321 atctccttca gaatatacac gtgaaatgat gagtaaatat atgactgaac taccttgcga
     4381 aacttgtcat ggaaagcgat tgagtcgtga agcgttatct gtttatgtag gtggtttaaa
     4441 tattggtgaa gtagtcgaat attcaatcag tcaagcgctg aactattata aaaacattga
     4501 tttgtcagaa caagatcaag cgattgcaaa tcaaatattg aaagaaatta tttcccgact
     4561 cactttttta aataatgtgg gacttgaata tttaacgtta aacagagctt caggtacact
     4621 ttcaggtggt gaagcacaac gtattcgatt agcaacgcaa attgggtcgc gtttgactgg
     4681 tgtcttatat gtattagatg agccatcaat tggactgcat caaagagata atgatcgatt
     4741 aattaataca cttaaagaaa tgagagattt aggaaatact ttaattgtag ttgaacacga
     4801 tgatgataca atgcgtgcgg ctgattactt agtggatata ggtcctggtg ctggtgaaca
     4861 tggagggcag attgtgtcta gtggtactcc tcaaaaggta atgaaagata aaaaatcatt
     4921 aacaggacaa tacttgagtg gtaagaaacg tattgaagta cctgaatatc gcagaccggc
     4981 ttcagatcgt aaaatttcta tacgtggagc tagaagcaac aatcttaaag gggttgatgt
     5041 ggacatacca ctatcaatca tgacggttgt tacaggtgta tcaggttctg gtaaaagctc
     5101 attagtaaat gaagtattat acaaatcatt agctcaaaaa attaataaat ctaaagtaaa
     5161 gccaggattg tacgataaga ttgaaggtat tgatcaactt gataaaatta ttgatattga
     5221 tcaatcacca ataggtagaa cgccacgctc taatccagca acatatactg gtgtgtttga
     5281 tgatatacgt gatgtgtttg cgcaaacaaa tgaagctaaa attcgaggat atcaaaaagg
     5341 gcgttttagt tttaatgtaa aaggtggacg ctgtgaagct tgtaaaggtg acggtattat
     5401 taaaattgaa atgcattttt tacctgatgt ttatgttcct tgtgaagtgt gtgatggtaa
     5461 acgatataat cgtgagacac tagaggttac ttacaaaggt aaaaatattg ctgacatttt
     5521 agaaatgact gttgaagaag caacacaatt ttttgaaaat attcctaaga ttaagcgcaa
     5581 gttacaaaca ctagttgatg ttggtcttgg atacgtcaca ttaggtcaac aagctacaac
     5641 gttatcaggt ggtgaggctc aacgtgtgaa acttgcatct gaacttcata aacgttcaac
     5701 tggtaaatct atttatatcc tagatgaacc gacaacaggg ttacatgttg acgatattag
     5761 tagattatta aaagtattaa accgattagt tgaaaatggt gatactgttg taattattga
     5821 acataaccta gatgttatca aaacagcaga ctatattata gacttaggtc ctgaaggtgg
     5881 tagtggcggt ggtactattg ttgcgactgg cacacccgaa gatattgctc agacaaagtc
     5941 atcatataca ggaaagtatt taaaagaagt acttgaacga gataaacaaa atactgaaga
     6001 taaataagat taaaagaagt gaaggatgtt ataaatttat ccttcgcttc tttttattaa
     6061 tttagtaatg aatagtagaa agaaaagatg cgtaaaaaga attatgttaa gataggttca
     6121 atctagagta gttaaacata aatcgaactg ggagtgggac agaaatgata aagaatcact
     6181 aatgatttat tatgtagtgg ttctttgtca ttagccacag ctattgtgta cttaaaaata
     6241 ggaatgcatg agtgcaactc atgcataaga aatactaatt tctaaagaaa aagtatttct
     6301 ttatgttggg gccccgccaa cttgcattgt ttgtagaatt tcttttcgaa attctttatg
     6361 ttggggcccc gccaactaat tccaatatat cattgtagag cttaggtcat tgatttttgg
     6421 ctcggacttt tatggcgata tgaaccatgt aaattaagca agcaataaat taatgattga
     6481 tattgacttg taaaataata acaataatga acaattaata tttattttag cttttcaatg
     6541 tagattggtg ttatattttt gatatgataa gaagagatgt aagagtaggg ataaatacaa
     6601 ttgaggtgaa cccatgttaa cgacagaaaa actagttgaa acattaaagt tagatttaat
     6661 cgctggtgaa gaaggactat cgaagccaat taaaaatgct gatatatcaa gaccgggctt
     6721 agagatggca ggttattttt cacattatgc gtcagataga atacaactat taggaacaac
     6781 ggaactatcg ttttacaatt tattaccaga taaggatcgc gcaggtcgta tgcgtaaact
     6841 atgcagacca gaaacgcctg caattattgt gacgcgtgga ttgcagccac cagaagaatt
     6901 agttgaagct gcaaaagaat taaatacccc acttatagtt gctaaagatg cgactacaag
     6961 tttaatgagt cgcttaacaa cgtttttaga gcatgcactt gcaaagacga catctttaca
     7021 tggtgtttta gtagatgttt acggtgttgg tgtactaatt accggtgatt caggaatagg
     7081 taaaagtgag actgcgttgg aattagttaa acgtgggcat agattagtag cagatgataa
     7141 tgtagaaata cgtcaaatta ataaagatga actaataggg aaaccaccaa agttaataga
     7201 acatctatta gaaatacgtg gactaggtat tatcaatgtt atgactttat ttggcgcggg
     7261 ttcaatatta actgaaaaac gaattagatt aaatattaat ttggaaaact ggaacaagca
     7321 aaagttatat gaccgcgtag gtcttaatga agagacgcta agtattttag atactgaaat
     7381 cactaaaaaa acaatacctg taagacctgg tagaaatgtt gcggtaatta ttgaggtcgc
     7441 tgcaatgaac tatcgattaa atatcatggg cattaacact gccgaagaat ttagtgaaag
     7501 attaaatgaa gaaattatca agaacagtca taagagtgag gagtaggttg aatgggtatt
     7561 gtatttaact atatagatcc tgtggcattt aacttaggac cactgagtgt acgatggtat
     7621 ggaattatca ttgctgtcgg aatattactt ggttactttg ttgcacaacg tgcactagtt
     7681 aaagcaggat tacataaaga tactttagta gatattattt tttatagtgc actatttgga
     7741 tttatcgcgg cacgaatcta ttttgtgatt ttccaatggc catattacgt ggaaaatcca
     7801 agtgaaatta ttaaaatatg gcatggtgga atagcaatac atggtggttt aataggtggc
     7861 tttattgctg gtgttattgt atgtaaagtg aaaaatttaa acccatttca aattggtgat
     7921 atcgttgcgc caagtataat tttagcgcaa ggaattggac gctggggtaa ctttatgaat
     7981 cacgaggcac atggtggacc tgtgtcacgc gcttttttag aaaaattaca tttgccaaat
     8041 tttataatag aaaatatgta tattaacggc caatattatc atccaacatt cttatatgaa
     8101 tccatttggg atgtcgctgg atttattatc ttagttaata ttcgtaaaca tttaaaatta
     8161 ggagaaacat tctttttata tttaacttgg tattcaattg gtcgattctt tatagaagga
     8221 ttacgtacag atagcttaat gctcacaagt aatattagag ttgcacaatt agtatcaatt
     8281 cttttaattt taataagtat aagtttaatt gtatatagaa ggattaagta taatccaccg
     8341 ttgtatagca aagttggggc gcttccatgg ccaacaaaaa aagtgaagta gtgatatttt
     8401 gagaaaattt ttatcaaaaa cacatcatca tacaaaccct ttatggcgtg tataccgtct
     8461 tgttaaattt tcgaaagttt ttaagaatgt aattatcatt gaattttcga aatttattcc
     8521 aagtatggta ctgaaaagac atatatataa acaactttta aatattaata tcggtaatca
     8581 atcgtcgata gcttataaag taatgttaga tattttttac ccagaactga ttacgattgg
     8641 tagtaacagt gttattggtt acaatgtaac aattttgacg catgaagcat tagttgatga
     8701 atttcgttat ggaccagtga cgataggatc taacactttg attggtgcaa atgctaccat
     8761 tttacccggt ataacgattg gtgacaatgt aaaagttgca gctggtacgg ttgtttcaaa
     8821 agatataccg gataatggat ttgcatatgg caaccctatg tatataaaaa tgattaggag
     8881 gtgacaattt tatggcgcaa aagaataata atgtaattcc aatgactttt gatgatgcat
     8941 tttatcgtaa aatggctaaa cagaagttta aacaaagaga atataaacga gctgctgaat
     9001 actttgaaaa agtgttagaa ttgtcacctg atgatctgga aattcaaatt gattatgcac
     9061 aatgtctagt gcaacttggt attgctaaaa aagcagaaca tttattttat gacaatatta
     9121 tttataatag gcatctagaa gatagctttt atgaattgag tcagctcaac attgaagtta
     9181 acgaaccaaa caaggcattc ttgtttggta ttaattatgt tattgttagc gacgaccaag
     9241 attatagaga tgaattagat caaatgtttg atgtgaaata tcaaagcgaa gaacaaattg
     9301 aacttgaagc tcaattgttt gtagttcaaa tactattcca atatcttttt tctcaaggtc
     9361 gattaaaaga tgcaaagaat tatgtcttac atcaaccaca agaagttcaa gatcatcgtg
     9421 tcgtacgtaa tttattggca atgtgttatt tatatctcgg tgaatatgat acggctaaag
     9481 cattgtacga agcactatta caagaggata gtacagatat atatgcatta tgccattata
     9541 ctttgctact ttataacact aaggaaaatg aacaatatca aaaatattta aaaatattaa
     9601 acaaagttgt acctatgaat gacgatgaaa gttttaaatt aggtattgta ttaagttatt
     9661 taaagcagta tcgtgcatca caacaattgt tgtacccttt atataaaaaa gggaaatttt
     9721 tatcaattca aatgtacaat gctttagcat ataattatta ttatttaggt gaagaagacg
     9781 aaagtcatta ctactgggat aaattgaagc aaatttctaa agtggaaatt ggacatgcgc
     9841 cttgggtaat tgaaaatagc aaagaagttt ttgaccaaca tattttgcca ttacttcaaa
     9901 gtgatgacag tcattatcgt ttatatggta tttttttatt ggatcaatta aatggtaaag
     9961 aaattgtgat gacggaaagt atttggcagg ttctggaaaa tctaaataat tatgagaaat
    10021 tgtatttaac gtatttagtt caaggtttaa cgctcaataa attagacttc attcatcgcg
    10081 gcttgttaac gctttaccaa aatgaattat ttgtaagtga aaatgattta atggttgcat
    10141 ggattaatca aggtgaactc ataattgctg aaaaagtaga tttaactgat gttgagccat
    10201 atatcggtgc gtttatttat ttgtatttta aaaatcaacc tcgaaacgtt acaaagaagc
    10261 aaattacaac atggttaggc ataacacaat ataaactgaa caaaatgatt gaatttctct
    10321 tgagcatata gatttatgaa aagttagatt tattatataa tgcgcataat gattaataat
    10381 gaggaggcgt taataaaatg actgaaatag attttgatat agcaattatc ggtgcaggtc
    10441 cagctggtat gactgctgca gtatacgcat cacgtgctaa tttaaaaaca gttatgattg
    10501 aaagaggtat tccaggcggt caaatggcta atacagaaga agtagagaac ttccctggtt
    10561 tcgaaatgat tacaggtcca gatttatcta caaaaatgtt tgaacacgct aaaaagtttg
    10621 gtgcagttta tcaatatgga gatattaaat ctgtagaaga taaaggcgaa tataaagtga
    10681 ttaactttgg taataaagaa ttaacagcga aagcggtcat tattgctaca ggtgcagaat
    10741 acaagaaaat tggtgttccg ggtgaacaag aacttggtgg acgcggtgta agttattgtg
    10801 cagtatgtga tggtgcattc tttaaaaata aacgcctatt cgttatcggt ggtggtgact
    10861 cagcagtaga agagggaaca ttcttaacta aatttgctga caaagtaaca atcgttcacc
    10921 gtcgtgatga gttacgtgca caacgtattt tacaagatag agcattcaaa aatgataaaa
    10981 tcgactttat ttggagtcat actttgaaat caattaatga aaaagacggc aaagtgggtt
    11041 ctgtgacatt aacgtctaca aaagatggtt cagaagaaac acacgaggct gatggtgtat
    11101 tcatctatat tggtatgaaa ccattaacag cgccatttaa agacttaggt attacaaatg
    11161 atgttggtta tattgtgaca aaagatgata tgacaacatc agtaccaggt atttttgcag
    11221 caggagatgt tcgtgacaaa ggtttacgcc aaattgtcac tgctactggc gatggtagta
    11281 ttgcagcgca aagtgcagcg gaatatattg aacatttaaa cgatcaagct taattcgagg
    11341 tcgaattaag atgttgagtt gtaagttatt tggatattta ttttaatagt gtcatcacat
    11401 tgttaaaata atgtcttact tttaaattaa aacaaattat atagaaaact agaacttagt
    11461 acgtatcatt tgtgcgtttc aatgagttct agttttttta tatgttatat taaacttata
    11521 actttatggg agtg
//
LOCUS       DAMZLE010000082        11420 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMZLE010000082 DAMZLE010000000
VERSION     DAMZLE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.083"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..639
                     /locus_tag="POB92_001829"
     CDS             1..639
                     /locus_tag="POB92_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDI6579314.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            642..1565
                     /locus_tag="POB92_001830"
     CDS             642..1565
                     /locus_tag="POB92_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6579315.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1577..2845
                     /locus_tag="POB92_001831"
     CDS             1577..2845
                     /locus_tag="POB92_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6579316.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2845..3468
                     /gene="udk"
                     /locus_tag="POB92_001832"
     CDS             2845..3468
                     /gene="udk"
                     /locus_tag="POB92_001832"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI6579317.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3496..3972
                     /gene="greA"
                     /locus_tag="POB92_001833"
     CDS             3496..3972
                     /gene="greA"
                     /locus_tag="POB92_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI6579318.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4298..5032
                     /gene="pxpB"
                     /locus_tag="POB92_001834"
     CDS             4298..5032
                     /gene="pxpB"
                     /locus_tag="POB92_001834"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDI6579319.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            5022..6032
                     /locus_tag="POB92_001835"
     CDS             5022..6032
                     /locus_tag="POB92_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDI6579320.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQLL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6034..6483
                     /locus_tag="POB92_001836"
     CDS             6034..6483
                     /locus_tag="POB92_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDI6579321.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6497..7858
                     /locus_tag="POB92_001837"
     CDS             6497..7858
                     /locus_tag="POB92_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6579322.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALGSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NFVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            7858..8610
                     /gene="pxpA"
                     /locus_tag="POB92_001838"
     CDS             7858..8610
                     /gene="pxpA"
                     /locus_tag="POB92_001838"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI6579323.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8622..9850
                     /locus_tag="POB92_001839"
                     /pseudo
     CDS             8622..9850
                     /locus_tag="POB92_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
     gene            10137..11357
                     /locus_tag="POB92_001840"
     CDS             10137..11357
                     /locus_tag="POB92_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDI6579324.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYTKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPI
                     IYEISDEFLNSIIK"
BASE COUNT         4139 a         1526 c         2219 g         3536 t
ORIGIN      
        1 atggatgacc taaataaaaa atatttaata gatttacatc aacatcaaaa tagttcaatc
       61 gaagttttgc gtgaatttgc cgaagtaaat gaagtgccaa ttgtagatcg tttaacatta
      121 gatttaatta agcaattaat tcgtatgaat aatgttaaaa atattttaga aattggtaca
      181 gcaatcggct atagttctat gcaattcgct tctatatctg atgacattca tgtcacaacg
      241 atagagcgta atgaaacgat gattcaatat gctaaacaaa atttagctac ttatcatttt
      301 gaaaaccagg ttcgaattat tgaaggtaat gctttagagc aatttgaaaa tgtaaatgac
      361 aaagtttatg atatgatatt tattgatgca gcaaaagcgc aatcaaagaa attttttgaa
      421 atatatacac cacttttaaa gcaccaaggt ctcgtaatta cagataatgt tttatatcac
      481 ggttttgtat cggatattgg gattgttaga tcgagaaatg taagacaaat ggttaaaaag
      541 gttcaagatt ataatgagtg gttaataaag caaccaggat atacaacgaa ttttttaaat
      601 atagacgatg gattagcgat ttcaattaaa ggagaatgac tatgacagaa ttacttgtca
      661 cacccaagtc attaagccat atggaaacac ttattgattt aggtgcagat gcttttgtaa
      721 ttggcgagca aaagtttggt ttaagacttc caggggaatt caatagacaa caaatgacag
      781 aggctgtcgc attagctcac aaaaacgata aaaaagtata tgcggcggtg aatggattat
      841 ttcataatta tcatttagat gctgtagaag attatattaa ttttttacat gaaatacgag
      901 ttgacagaat tattttcggg gatccagcag tagtaatgta tgtgaaggcg caagagaatc
      961 caataccact tcattggaat gcagagacgt tagtaactaa tcattttcaa tgtaattact
     1021 ggggtaaaag aggcgcatca agagctgtct tagcacgtga gctgaatttg gatgaaatta
     1081 ttaatataaa agaaaattca aatgtggaaa ttgaggtgca agttcaagga atgacttgta
     1141 tgttccaatc aaagcgtatg ctactaggca attattatac gttccaagat cgccaaatga
     1201 aaattgaacg tcgcaatgat gaacaatctt tattacttta tgatgaagaa agacaaaata
     1261 attacccagt ttacgaagat tacaatggta cgcacattat gtcaccaaac gacatttgtt
     1321 taattgaaga attagcacca ttttttgagg cgggtataga ttcatttaaa atcgatggta
     1381 ttctacaaac ggaagaatat attaatgtgg taacggaaca gtatcgtcaa gcgatagatt
     1441 tgtacaatga agatcctgaa atctatgaag atgagaaatt tatgttgatg gacccaattg
     1501 aagaaattca acctgatcat cgaccatttg acgaaggttt cttatataaa caaacggtat
     1561 attaaggagg ttaatcatga agacaataga agagattaaa tcaactccta aaacagttat
     1621 gaagaaacca gaattattag cacctgctgg aaacttagaa aagttaaaaa tagcagtaca
     1681 ttatggcgct gatgccgtat ttttaggtgg tcaagaatat ggattacgtt caaatgctga
     1741 taatttcacg atggaagaaa tagctgaagg tgttgaattt gcgaaccgtt acggtgccaa
     1801 aatttatgtt acgacaaata ttattgcaca tgatgagaat attgaaggtc tagaatcata
     1861 tttgcgtaat ttggaaaaga ctggtgcgac aggtatcatt gttgcagatc ctttaattat
     1921 cgaaacatgt aaagaagttg cgccaaaact tgaaattcat ttatctactc aacaatcact
     1981 ttctaattac aaagctgtag aatattggaa agaagaagga ttggatcgtg ttgtattagc
     2041 acgtgagacc ggcgcgatgg aaatgcgtga aatgaaggaa aaagtagata ttgaaatcga
     2101 agcatttatt catggtgcta tgtgtatcgc ctattcaggt agatgtacat taagtaatca
     2161 tatgactgca agggattcca acagaggcgg ttgctgtcaa agttgccgtt gggattatga
     2221 attattagaa gttgatgata atggtgaact tgatgttttt tataatcaag gtgaagttac
     2281 accgtttgcg atgagtccta aagatttaaa attaatcgaa tcaattcctc aaatgatgga
     2341 tattggtgtg gactcattaa aaattgaagg acgtatgaag tcaattcatt atattgcaac
     2401 agttgtctca gtatatcgta aagtcattga tgcgtatgcg gcagatcctg acaattttaa
     2461 gattaatccg gaatggttaa tagagttaga taaatgtgca aatagagaca ctgcaccagc
     2521 attctttgag ggaacacctg gttatgaaga acagatgttt ggtcaacaac aatctaaaaa
     2581 atcaccattt gatttttgtg gtttggtatt agactataat gaagatacaa aaattgcgac
     2641 tattcaacaa cgaaataact ttaaaccagg tcaagaaata gaattctttg gtccagaaat
     2701 tgaaacattt acacaagtag tagaagcaat ttatgatgaa gaaggtaaca gcttagacgc
     2761 ggcgcgccat ccattacaaa tcgttcaaat taaagtggat cgcccgatat atccaaacaa
     2821 catgatgaga aaggaaattg gctaatgaaa gctactacaa tcattggcat agctggtgga
     2881 tctggctcag gaaaaacaac tgtaactaac gaaattatga aaaacttaga aggtcatagt
     2941 gtcgctttac ttgctcaaga ttactattat aaagatcaaa agcacttgac tttcgacgag
     3001 cgcctagaaa ccaattatga ccatccattt gcattcgata atgatttatt aattgaaaat
     3061 cttaaagact tgaaaaatgg taaagcagta gaagtaccga catatgatta tgctagtcat
     3121 acaagaagtg acattaccat tgattttaaa cctaaagatg ttattatcgt agaaggtatt
     3181 ttcgctttag aaaataaggt attacgtgat atgatggatg ttaaaatata tgttgataca
     3241 gatgcagact tgagaatatt acgccgttta acacgagata ctaaagagcg tgggcgttca
     3301 atggactctg ttatcaatca atatttaagt gttgttagac ctatgcatga ccaatttatt
     3361 gaaccgacta agaaatatgc tgatataatt attcctgaag gtgggagcaa taaagttgca
     3421 atagatatta tgacaacaaa aattcagtct ttagttagca agcaatagta taattcagta
     3481 aaggaagatg acaatatgga aaatcaaaag caatatccaa tgactcaaga aggttttgaa
     3541 aaattagagc gtgaacttga agaattaaaa acagttaagc gtcctgaagt tgtagagaaa
     3601 attaaagttg cacgttcatt tggtgactta tcagagaact ctgagtatga tgcagcaaaa
     3661 gatgaacaag gattcatcga acaagatatt caaagaattg agcatatgtt aagaaatgca
     3721 ttaatcattg aagatactgg agataacaac gttgttaaaa ttggtaaaac agtaacgttt
     3781 gtagaattac caggtgatga agaggaaagt tatcaaatcg ttggttcagc tgaatcagat
     3841 gcatttaatg gtaagatttc aaatgaatca ccaatggcta aagcgttaat tggtaaaggt
     3901 ttagatgatg aagttcgtgt tccactacct aatggtggcg aaatgaacgt aaaaattgtt
     3961 aatatccaat aaatgatcaa atagattgtt taattgcgcg attgaagtgt gttaatgata
     4021 cttcggtcgt gcttttttat attcaaaatc aaaaaatcgg acagatgaaa agtaaaaatt
     4081 ttccatcaga ccgaatgata gcagaaccta aattagtgta agagttacaa tattaactac
     4141 tccattttaa aaaagcaaaa tatttccgag aaaacaaatt gattatcatt tgttattgta
     4201 tgcgatttga attacttatt aaacaacttg tgcttctcaa tgatacaatg aaaaggtatt
     4261 caatactggt ttattgcaat acaaaggggg atttaaagtg gatgtaagat ttattaacga
     4321 gcagacaatt atgatttatt ttgaaaataa aatatccgaa gagacatatc gtaatgtcac
     4381 agcaatggta cggtggatac gagaaaaaga aatattagaa attcaagata ttgtcccttc
     4441 gtatcgagca gttctaatat attttgatga acaagctata acatcttcta aattaattga
     4501 aaatttagaa cttaacaaat ttaatgaaaa aaatgttcat gctgtgaatc aaaccaatcg
     4561 tattattaaa atacctgttc aatatggtgg tacatatgga ccagatattg aagaagtagc
     4621 aaagcataat cgaataactg ttgagcaagt tattgaaaaa catacaagta aaccttattt
     4681 aatatatatg ctaggattta tgccaggatt tccatactta ggcggacttg atgaacaact
     4741 acatacacct agacgtaatc agccaaggtt aaaaattcat gcaggttctg taggtattgc
     4801 gaataatcaa acaggactat atccatcaga ttctcctggc ggatggcaaa ttattggacg
     4861 cacaccgtta aaagtattta gcagcgagcg tgaaccaatg tcaatgtacg aagcaggaga
     4921 atggatacaa ttttatgcaa tagatgaaca agaatttata caaatcgaaa gagatatatc
     4981 agatggcaac tttaatgttg atgactgggt ggtgattgaa aatgtcaatt aagattttac
     5041 aaccaggtct cttttcaacg gtacaagatc taggaagaaa aggttatgaa catattggat
     5101 tttcaggcgc aggtgccatg gatcaattta gttttaaggt tgcgcagtta ttaattaaca
     5161 atgatggtcc agcgattgaa tatactttga ttggtcctac cattcaattt aatacgcaaa
     5221 atacatttgt tataaccggt ggtagtgtta atgcctcgct aaataataaa actatatcaa
     5281 tgaattctgt catattagct gagaaaggtg acattttaaa aataggtgct ataaccaaag
     5341 gtgcacgcgg ttatcttact tttggtcatt ctatcaacgt acctcctatt gctgaaagtt
     5401 atgcaacaca tacccgaagc agtataggag gattcaaagg tagaaagtta ttagctaatg
     5461 atgtaataac agtgaaaata aataatgact ttaaagaaaa cattggaaag acgattcatt
     5521 tacaggacga tttattgcca gaaaataata ttatacacat tcttcaagga cctcaattcg
     5581 aggcattttc tgaagaggct agagcgaaaa ttgtaaatca tccatattta attactgaac
     5641 aatcagaccg tatgggctat cgtttggaag gtgacagcgt tgcaccaatt aatcaagcag
     5701 atatcatttc tgaaccggtt gctcttggta gtgttcaagt accaaatgat ggtcaaccta
     5761 ttattctgct taatgataaa caaacgatag gtggttatac caaaattgca actgtatgca
     5821 aatttgatct gccaaagtta gcacagatga aaccacaaga tacaattcaa tttaaatgga
     5881 tatcattcca agaagctgta gataagaatc gggaacaaat gtctctgttt aatgaaattc
     5941 ttaagagtca tcaaaagact ccaatttttg atacatctag cttgcgacat acttcgaaaa
     6001 aattagcaac aattttaaaa ggggatttgt aaaatgaata ttgaaaaaat cgaacaaata
     6061 atcaaattag tgaaggaaaa tgatgttaag aaatttaaat ataaaaattt tgaagatgaa
     6121 atagaaattg acttcactga ctcgaatcat ttggctgcac acagtaatca atcaaatcaa
     6181 agtatgaaca ataatgattt gacagcttca aaagcgaatg ataactccga tgtttcgaca
     6241 aatgattatc atgacattaa atcaccaatg gtaggtacat tctttttaca agatagtaaa
     6301 gaattaactg aaccaattgt gaatgtcggt gacaaagtta acaagggaga tattatagga
     6361 tatgttgaag cgatgaaagt attaaacgag gtaacaacag atgttgctgg agaaattact
     6421 gaaatagtag ctgatcatgg aacaaatgtt gaatacgacc aagttttggt acgtattaag
     6481 taaagggggt aagtaaatgc ttcgttgttt aattgcgaat agaggtgaaa ttgctgtaag
     6541 gattataaga gcttgcagag aatatgggat tgaaactgta gcagtttatg caaaagggga
     6601 cgagcaaagc ttgcatgtac atttagcaga tcaagctata tgtattgggg aagctaatgc
     6661 tttaggtagt tatttaaata ttgaccgcat catatctgct gcacaaatca ctggtgctaa
     6721 tgcaattcac ccaggatatg gctttttatc agaatcaaca aaatttgctc aaaccgttga
     6781 ggaacaaggc atagcgttta tagggcctac taaaaaaacg atggaaatga tgggggataa
     6841 aataacagca agacaaacag ttcatcatgc aggtgttcct gtgattccag gatcaaatgg
     6901 tgctgtgaac catgtgtctg aaattgagaa gcttgccaaa gacatcggtt atccagttgt
     6961 tatcaaagct gccagtggcg gtggtggtaa aggtatacgt attgtaaaga aagctgaaga
     7021 tttagaaaag gcatttaaag aagctaaaag tgaaggaaaa aaatactttg atgatgatcg
     7081 tgtttatgtt gaggctttta tacctgttgc aaaacatgtt gaagttcaag ttatgggaga
     7141 tgggcaggat aattttgtac atttagggga acgtgattgt tctgtacaac gtaaaaatca
     7201 aaaattaatc gaagaatctc cttgtgcagc attaactgaa gaaagacgac aacaaatatg
     7261 taacgatgct gttaaagttg cacgtgcagc aaattataga agtgcaggta cgattgaatt
     7321 tttagtaact gataccgcac attactttat tgaaatgaat gctcgaatac aagttgaaca
     7381 tactgttaca gagatgcgag cagaacgtga tttggttgca gcacaattgt atttattgga
     7441 acataatcat ttaccttttt cacaaagcga tattcaattt aatggtcatg tgatagaagc
     7501 gagaataaat gccgaaaatc ctgaaaagaa atttcaacca acaccaggta aagtgacagc
     7561 gttacattta cctcaaggct ttaatgtgcg agtcgattct ttactatatc acggttatca
     7621 agtttcacca tattatgatt ctttagtagc taaagtaatt gtaaaatctc atgatagagc
     7681 ttcagcaatt gataagttga aagccacctt agatgaaatg gtgatagatg gattttctac
     7741 aactgccgat tttttatatg cagttttaaa ttatccacta tatcgtgatg gagatgcaaa
     7801 agatgttgat attaaatttt tagaaaaaca tcaaattgtt aaaggggtgg aattataatg
     7861 cgagttgatt tgaattgtga tttaggcgaa gcatttggaa attattcctt tggtggtgat
     7921 catcaaatta ttccgttaat tacaagtgcg aatgttgctt gtggttttca cgctggtgat
     7981 gaaaatgtaa tgaatgaaac ggtaaaactt gccaaagcac ataatgttgc agtaggtgca
     8041 catcctggtt tacctgattt gaaaggcttt ggcagacgaa atatagatat ctctaacgac
     8101 gagatttata atttgatgat ttatcaatta ggtgcattac aagggttttg tcgcattcat
     8161 caagttaaaa ttaatcatgt taaaccgcat ggtgcgttgt atcagatggg tgcaaaagac
     8221 agagaaatag caagcgttat agcacaagct gtttatgact ttgatccatc actagtgtta
     8281 gtaggattag caaattcata tctaatttca gaagcaaaga atgtcggatt aattacagct
     8341 tctgaagtgt ttgctgatag acgatacgaa gatgatgggc agctcgttag tagaaaagaa
     8401 agtgatgctg tgattactga tactgacgaa gcacttaagc aggttttaaa gatggtgaag
     8461 gaaaataaag ttatttcaaa aaacaataag gaagtaacgt tacaagcaga tacaatttgt
     8521 gtgcatggtg atggagaaca tgcattatta tttgtttcga aaattagaga aattttaatg
     8581 aaagaaggca ttgatattca atccttatag ggggctaaaa aatgggaaga aatttaaagc
     8641 ttaaaaaaga aagtgacttt gaattcacaa aaaatcataa aaggttatta ttaggttctg
     8701 tatttttgat ggcaacttct gcaattgggc cagcattttt aacgcaaaca gcagtattta
     8761 catcacaatt ttttgcaagt ttcgcatttg ccatattact gtctatcatc attgacattg
     8821 gtgcacaaat taatatatgg cgcatattag ttgtaactgg tttaagaggt caagaaatat
     8881 caaataaagt tgttcctggg cttggtactg ttatctcaat attgattgca tttggtggtc
     8941 tagcttttaa tattggtaat attgctggtg ccggtttagg tttaaatgca atttttggat
     9001 tagatgtaaa atggggcgca gctattactg caatctttgc aatattaatc tttgtaagta
     9061 aaagtggcca aaaaattatg gacgttgttt caatgattct tggtattgtg atgattttag
     9121 ttgtggcata tgtgatgttt gtttctaatc caccttatgg tgatgctttt gtgcatacat
     9181 ttgcgccaga acatccaatg aaattagtct tgccaatcat tacgttagtt ggtggaactg
     9241 taggtggtta tattaccttt gcaggtgcac atcgtatatt agactctggc attaaaggta
     9301 agcaatattt accatttgta aatcaatcag caattgctgg tattttaact acaggtatta
     9361 tgagaagtta ctattcctag cggtattagg tgttgttgta acaggtgtga cactaagttc
     9421 tgaaaatcca ccagcgtcag tttttgaaca cgcaattgga ccaattggaa agaatatttt
     9481 tggtattgtg ttatttgctg cagctatgtc atcagtaatt ggctcagcat acacaagcgc
     9541 aacattttta aaaacacttc ataaatcact taacgaaaga agtaatttaa ttgtgattgt
     9601 gtttatcgtt atttcaacaa tgattttctt atttattgga aaaccaatca gccttttaat
     9661 tatagcaggc gcgataaatg gatggatttt acctattacg ttaggtgcaa ttttgattgc
     9721 aagtaaaaag aaatcaattg ttggtgatta taaacaccca aattggatgt tcatctttgg
     9781 tatcgttgca gtacttgtta caatattgac aggtatattc tcattcaaag aagtacttca
     9841 gttattttaa aagacatttt gactaatagc atcactgtaa atgttgttag tattaggcaa
     9901 gatgtatttt gatttccggt gattcattta taaagcataa ttagatatat ttgttgagag
     9961 tggtgcatga caaatgtact gctctttttg ttgtgatgaa acttctgttt cagttgtttg
    10021 gataaataga tatttatata gtttttaaag aaaaaaagtg ataatattta gctaatctaa
    10081 tgaaaatgga tttattggac aaaaaataca ttttaaatga gtagaggagg ctgcaaatgg
    10141 gttacagaac tttaaaaagt atttttcatg aacacaatga aagtaaaatg aaggaagagt
    10201 atactaaaag atttaattct ttagcttctt tcaatactaa tattaatatc atacctatgg
    10261 aaaatggaaa aaaagttaat gatttggaat atcctctatt ctttatggtg actaaaaatc
    10321 tatcaaaaaa acaagaatta atatcaatta atagtagaaa aattgataga gcacttaatt
    10381 ctttaccata tgcagctaga gaacaatatt ttaatgattt attaatcgat gaattacaaa
    10441 gtactaatga aattgaaaat gtatttagta ctaaacaaga gattgcacat gcgttaaata
    10501 accaagcatc agaatttctt aagttcagag gcctcgtgga tcaatataaa gagatagaac
    10561 ttaataaaaa aattaaagtt gataatgtaa gagacattag agcgatttat gataaattag
    10621 tttcaaatga aattaacgaa caagataagt tagatggaga gctatttcgt aaaaattttg
    10681 tcggtgtgca tgatgggtca acgaataaat atatacatgt tgggttacaa cctgaaacca
    10741 aaattgttga atatataggt gaaatgctaa catttttaaa atattttgat gcgcctcagc
    10801 cgttcaaaat catggctagt cattatctgt ttgaatatat acatcctttt tatgatggca
    10861 atggtagagt tggaagattt ataattgcca agctcttgag tgattattat gataattata
    10921 ctgctttgac attttcatat gttataaata gaaataaatc gaaatattac aaggcgttta
    10981 tgactgcttc aaatcatttg aattgtggtg atttgacgga atttattgac acaatgcttg
    11041 agctattgat agcaggacaa gaaagaatac ttgatgagtt aataccaaaa atggatgcga
    11101 ctgaaaaatt aacgctttat ttaactagtc attataaaca gatagattat gaatttttgt
    11161 atctcctttc aatggataaa ttgtttggaa ataaaagaaa tagattaaca ttaattgatt
    11221 tagaaaacat attgggagta ggaagagtaa aaattaataa tacaattaaa aaatatgata
    11281 actacttagt taaaattaaa agtagaccaa taatttatga gataagtgat gaatttctaa
    11341 attctattat aaaataaata taatatgtga ttcagctata cttatagtat ttgtaagatc
    11401 gcgtaattgc ttcattgaaa
//
LOCUS       DAMZLE010000083        11287 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMZLE010000083 DAMZLE010000000
VERSION     DAMZLE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.084"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            211..927
                     /locus_tag="POB92_001841"
     CDS             211..927
                     /locus_tag="POB92_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6579325.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            927..1400
                     /locus_tag="POB92_001842"
     CDS             927..1400
                     /locus_tag="POB92_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDI6579326.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEFSNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1492..1608
                     /locus_tag="POB92_001843"
     CDS             1492..1608
                     /locus_tag="POB92_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579327.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1753..2397
                     /locus_tag="POB92_001844"
     CDS             1753..2397
                     /locus_tag="POB92_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI6579328.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2513..3379
                     /locus_tag="POB92_001845"
     CDS             2513..3379
                     /locus_tag="POB92_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6579329.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3718..4938
                     /locus_tag="POB92_001846"
     CDS             3718..4938
                     /locus_tag="POB92_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDI6579330.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4935..5423
                     /locus_tag="POB92_001847"
     CDS             4935..5423
                     /locus_tag="POB92_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDI6579331.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5522..6211)
                     /locus_tag="POB92_001848"
     CDS             complement(5522..6211)
                     /locus_tag="POB92_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDI6579332.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6338..6781
                     /locus_tag="POB92_001849"
     CDS             6338..6781
                     /locus_tag="POB92_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579333.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6848..7243
                     /locus_tag="POB92_001850"
     CDS             6848..7243
                     /locus_tag="POB92_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579334.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            7381..7680
                     /locus_tag="POB92_001851"
     CDS             7381..7680
                     /locus_tag="POB92_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579335.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7760..8302
                     /locus_tag="POB92_001852"
     CDS             7760..8302
                     /locus_tag="POB92_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579336.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8398..8964)
                     /locus_tag="POB92_001853"
     CDS             complement(8398..8964)
                     /locus_tag="POB92_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDI6579337.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8966..9424)
                     /locus_tag="POB92_001854"
     CDS             complement(8966..9424)
                     /locus_tag="POB92_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI6579338.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9427..10110)
                     /locus_tag="POB92_001855"
     CDS             complement(9427..10110)
                     /locus_tag="POB92_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579339.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(10282..11157)
                     /locus_tag="POB92_001856"
     CDS             complement(10282..11157)
                     /locus_tag="POB92_001856"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6579340.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQITDIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT         3964 a         1589 c         1951 g         3783 t
ORIGIN      
        1 gaacaatttt taaatattta attgaaattg agtgtcgaaa gcatagaatt tgcttatcgg
       61 cacattttta atttatacat attttaaaac taagtaacag tttgaaggaa tcgtagttca
      121 ataatgttaa ttgtgaaaat gtatataaac ataaaaaaat catgtataat atatgttgtt
      181 aattaaacag ttcgaaagcg agatgacatt atgggacgta aatggaataa cattaaagaa
      241 aaaaaggccc aaaaagataa aaacacaagt agaatatatg cgaaatttgg taaggagatt
      301 tatgttgcag caaaatctgg tgaacccaat ccagaatcta accaagcttt aaggttggtg
      361 cttgaacgcg ctaagacata ttcagtgccg aatcatatta ttgaaaaagc aatagataaa
      421 gctaagggtg ctggagacga aaactttgat cacctaagat atgaaggatt tggcccaagc
      481 ggatcaatgc taattgttga tgcgttaaca aataatgtaa atcgtactgc ctctgatgtg
      541 cgagctgctt ttggtaaaaa tggcggtaat atgggtgtat ctggatcagt tgcttatatg
      601 tttgatcatg tggcaacatt tggtattgaa ggaaagtctg ttgacgaaat acttgaaaca
      661 ttaatggaac aagatgtaga tgtaaatgat gtgattgacg ataatggatt gacaatagtc
      721 tatgctgaac cagatcaatt tgcagtcgtt caagatgcgc ttcgtgcagc aggtgttgaa
      781 gaatttaaag ttgctgaatt tgaaatgtta cctcaaacag atattgaact ttctgaagcg
      841 gaccaagtaa catttgaaaa attaatcgat gcattagaag atttagaaga tgtacaaaac
      901 gtattccata atgtggattt gaaataatga aatcagcaga acaatggatt gatgaattgc
      961 aacttgaatc acatcctgaa ggtggtttct atagagagac aattcgagaa gtattgaaag
     1021 atggacgcag agcgccgttt agtagtattt attttttact tacagatgac aatatttcgc
     1081 attttcatcg aattgatgct gatgaagtat ggtactatca tgctggcgat tctctaacaa
     1141 ttcatatgat aaatccggat ggggaatata cgactgcaac attgggtact gatatccaaa
     1201 atggagatgt attgcaatat gtagtgccta aaggaacaat ttttgcttct tcaatcgaat
     1261 tttcaaatac ttatagttta gtaggttgta tgtgtcaacc ggcatttgag tttaagcagt
     1321 ttgaattgtt taagcaatct gaattaatta cacaatatcc gcatcttaaa tcagtgattg
     1381 aaaaatatgc tttaaaataa aaatgatcaa tgaagtggtt tgaaggttgt taataaacct
     1441 ttgagtcact tcatttttgt atgtattctt gattgaatca gaatagaatt gatgctacag
     1501 ctgtttttaa tgaaaatagc attaaatgat tttgaaaacg ataagagtgt gttatttata
     1561 tttttgaaaa atcactttta tgaagaaatg tgtgtactat ctaattaata ttgtctattt
     1621 taagtaaatt gaagcgagtt gtagtaaaat aataataaat attttcatat gattaacaaa
     1681 aactataaaa ctgtatcatg acatgacatc atttatgtag ttttatataa taatgacaag
     1741 gagttggtga ctgtgaaagt aaagtatata gataaacgtc actggcgtcg cctaattgat
     1801 agggaataca cagaggtaaa agttaataat aataggttta agggtattat aggcttagtc
     1861 acgatgaaaa aggttcgtga tcctttagag gtgacggtag ttggacaaaa tatcattgtc
     1921 gcagatgaca attataaatg gttgcaaata ctacctgaaa agaaacgtta tagtataact
     1981 gtaatgtttg ataataaagg caatccatta gaatattatt ttgatataaa tatcaaaaat
     2041 ataacgcaaa aaggtaatgc gcgtacagta gatttatgtt tagatgtttt agcgttacca
     2101 agtggtgaat atgagttggt agatgaagat gacttaatgt ttgcattaga aagtgagcaa
     2161 attacaaaaa agcaatttca tgaagcatat atgattgcac atcaaattat ggcagagtta
     2221 gaaaatgatt ttaaaggatt ccaaaagaaa atcatgtact gctttaataa aattaatgca
     2281 aaggctcaaa aaaatcatca aaagccacaa aataaaacta atattgaaaa aagcaaacaa
     2341 ataaagccta agcaatataa tcaaactaaa aatcaccaac aacaaaagaa aaactaagta
     2401 attcaagctg cagccatgcc aataaaattg gtgaaattat tcgttagtaa tgaactcttg
     2461 aatgctcgta gtcatgaaag ttcaataatt gaataattta tggggggaaa ttatgaagat
     2521 tgaagactat cgtttactaa taacattaga cgaaacgaaa acgttacgta aagcggctga
     2581 aattttatat atatctcaac ctgctgttac acaaagacta aaagctattg aaaatgcttt
     2641 tggagtagat atttttatca gaacaaaaaa acaattgatt acaacaactg aaggaacaat
     2701 gattattgag catgcccgtg acatgttgaa aagagagcga ttattttttg acaaaatgca
     2761 ggcacatatt ggtgaagtga atggaacaat atcaatcggg tgttcttctt tgattggaca
     2821 aaccttactt cctgaagttt tgagcctata taatgcccaa tttcctaatg ttgaaataca
     2881 agtgcaagtt ggttcaactg aacaaattaa agcaaatcat agagattatc atgttatgat
     2941 aactcgtgga aataaagtaa tgaatttagc taacacacat ttatttaatg atgatcatta
     3001 ttttattttt ccaaaaaata gacgagatga tgttacaaag ttaccattta tagagtttca
     3061 agctgatccg atttatataa atcaaataaa acaatggtat aacgataatt tagaacaaga
     3121 ttaccatgca actattacag tggatcaagt agcaacttgc aaagaaatgt tgattagtgg
     3181 tgtaggtgtt acaattttgc cggaaattat gatgaaaaat atcagcaaag aacaatttga
     3241 gtttgaaaaa gtagaaattg ataatgaacc gctgattcgt tcgacattta tgagttatga
     3301 tccgagcatg ttgcaattgc cacaagttga ttcttttgta aatctcatgg cgagctttgt
     3361 tgaacaacca aaggcgtagt tttagactaa tttaaggttt gtatttagtt ttaaactatt
     3421 cggttaaatt gaacgtagtt ggttgctaat gcaccaacag caaaagagcc cctaattaat
     3481 aaattaaaag gggacaaagg aatacagttg gttgctaatg caccaacagc aaaagagccc
     3541 ctaattaata aattaaaagg ggacaaagga atacagttga ttgctaatgc accaactgca
     3601 taagagcccc taattaataa attaaaaggg gacaaaggaa tacagttgga tgctaacgca
     3661 ccaactgcat aagagcccct aattaataaa ttaaaagggg ctctaagaag cggggttatg
     3721 gatatgtttg cagcgttatt acaaataaag aattataaac tctttgttgc taatatgttt
     3781 ctactaggta tgggtattgc ggttacggtc ccatatcttg ttctttttgc aactaaagat
     3841 ttaggtatga caacaaatca gtatggatta cttctagcat ctgcagcgat tagccagttt
     3901 acagtaaatt caattattgc tagattttcg gatacgcatc actttaatag aaaaattatt
     3961 attattctcg cattattaat gggtgcgctt ggtttttcaa tatacttttt tgtagataca
     4021 atctggttat tcatattact atatgcgatt ttccaaggat tatttgcacc agcaatgccc
     4081 caactttacg catctgctag agaatctatc aatgtttcaa gctctaaaga tagagctcaa
     4141 tttgccaaca cagtattacg ttcaatgttc tcattgggct ttttatttgg tccatttatt
     4201 ggtgcccaat taatcggttt aaaaggctat gctggattgt ttggtggaac aataagtatc
     4261 attttattta ctttagtact tcaagtgttt ttctataagg atttaaacat taaacaccct
     4321 attagtacgc aacaacatgt tgaaaaaatt gctcctaata tgtttaaaga caaaacgctt
     4381 ttattaccat ttattgcatt tattttatta cacattggac aatggatgta tacgatgaat
     4441 atgcctttat ttgttactga ttatttaaaa gaaaatgaac aacatgtcgg ttatttagct
     4501 agtttatgtg ctggtttaga agtgccattt atgatcattc ttggcgtttt atcatctaga
     4561 ttacagactc gaacattgtt gatttatgga gcgatttttg gtggtttatt ctacttcagc
     4621 attggggtat ttaaaaactt ctatatgatg ttagcaggac aggtgttttt agctattttc
     4681 ttagcggttc ttttaggaat tggtattagt tatttccaag atatcttacc agattttcca
     4741 ggatacgcct caacactatt ttctaatgca atggttattg gacagttagg cggtaaccta
     4801 ttaggtggtg ctatgagtca ctgggtaggt ttggaaaatg tattttttgt atcagcagca
     4861 tcaatcatgt taggtatgat acttatattc tttactaaaa atcaaaaaat tacaaaagag
     4921 gatgtgatat caacatgaca attattttat ggctacttat catcgctgcc ttcatgttag
     4981 catttgttgg gttgattaag ccgattattc cttctgtttt agtattatgg gttggctttt
     5041 taatctatca atttggcttt cataatcagc atttatcatg ggtgttttat gtatctatgg
     5101 cattgctaac aatattaatt ttatgtgccg actttttagc taataaatat tttgtgaatc
     5161 gcttcggtgg ttctaagttt ggagagtatg cagctttaat tggtgtggtt attggatgtt
     5221 ttgttttacc gccatttgga attattatta taccttttat tttggtattc atagttgaat
     5281 taatacaagg ctattcattt gaaagagcag ttaaagtaag tataggttca atcgtagcat
     5341 ttttaacaag tagtatagct caagcaatca ttatgtttat aatgattgta tggttcttta
     5401 tagatgcttt attgattaat taataaaaag cttattgcaa aatatgtttt tcggtaactg
     5461 taatttagtg attttatcat taacagtacc aaattcgcat atgatgtaat aagctttttg
     5521 tttataaaaa tgtatgagaa tgttttttcg aaatatttct ttcaatgcgt aatccaatag
     5581 gcataactat aaatgaaaat ataataaata cccaattgct tacctgaata tatggtccaa
     5641 aggcaagtaa tgattgtgga ataaagaata catagaaaaa tcctacaatt agtagaatga
     5701 ttgctaaata ggtgaatatc catatccaat ttgaattgtt aaaacactgc agttgtattg
     5761 ttttaaaact aaaccatata aataaaaaga caataaagaa tccaacgact actgaaaacg
     5821 ggaatgaaac aaaatataaa ttacttccat ttttttccat gaaaaatcct aaaaatcctt
     5881 tgagaaaact aacaatccca attaatagaa cgatgtgttg atagatatat ttaaaaatat
     5941 ttttaaatgt ttcattaggc atcgctttta gttcttttat tgcatgtgct tttgggtcgt
     6001 gattgaaaaa atctaaggct aataaaccat gttgttctgc gcttaataat tgtttgagta
     6061 tacggttaat aattaactct gtatcatgag ggttgacgcg aaagtcagag cgcatataag
     6121 tcatataatt ctcgaagatt tctctatcag tattgcttaa tcttaatgat ttaacattat
     6181 tttcttttgt taattgcgca gtacttttca ttgttactta agcgctcctt taaaaatgtt
     6241 taattccaaa ttaaaatgga aatgatttta tagtattaat aaggtcaatc atatcatatt
     6301 aaacgcataa atataacgat taatattgga gaggaaaatg aggacactta ataaagatga
     6361 acataattat atcaagcaaa tagctaatat acatgagaca ttattgtcgc aagtagaatc
     6421 caactataaa tgtactaaac tgagtattgc tcttaggtac gagatgatat gttcaagatt
     6481 agaacataca aatgataaaa tttatatata tgaaaatgaa ggtcaattaa tagcgtttat
     6541 ttggggacat tttagtaatg aaaaaagtat ggttaacatt gaactgctat atgttgaacc
     6601 acaatttcgc aaactgggaa tagctacgca actgaagatt gcgcttgaaa aatgggcaaa
     6661 aactatgaat gcaaagcgaa taagcaatac aattcataaa aataatttgc caatgatatc
     6721 tttgaataaa gatttaggtt atcaagtgag tcatgtgaaa atgtataaag atattgatta
     6781 gaattaggat tatgttgcta attcatgtta aaattaaaaa agatttaatg acgttaagga
     6841 gttttatatg aagaaattaa tcatcagtat tatggcggtc atgctatttt taacaggttg
     6901 tggtaaaagt caagagaaag ccactctgga aaaggatatc gataatttac aaaaagaaaa
     6961 taaagaatta aaagacaaaa aagaaaagct tcaacaagaa aaagaaaaat tagcagataa
     7021 gcaaaaagac cttgaaaaag aagtgaaaga tttaaaacct tcaaaagaag ataacaagga
     7081 tgataaaaaa gacgaagaca aaaataaaga caaagataaa gataaagagg catcacaaga
     7141 taagcaatca aaagatcaaa ctaagtcatc ggataaagat aatcacaaaa agcctacatc
     7201 agcagataaa gatcaaaaag ctaatgacaa acaccaatca taatcgaatt gcttacttgt
     7261 tatagatgaa aggtacagcg ttttaaacct tattttaagg gtatgtatta attaaaatgt
     7321 ggtcatgatt gaaacagaac gtaaaaatag acaacgtaat taataaagga gagaaacggc
     7381 atgcatgaac aagattttag aattttagag ggtcaagata ttactttgcc agaattaggt
     7441 agagaattag aaaatattac aggacatacg attgctgatt ctactggcga aattaagcgt
     7501 gtaattgcac atttaccaaa ctttgagtcc gatacagata cttttgttgc tacatatcgt
     7561 ttaaaccatc aacaagattt tatagatgca acttttactg cgctgaaatc agatagagca
     7621 cgtttaaaag aagtgccagt tcatgttgaa cttataagtt atatttctaa atcaaaataa
     7681 actgctatct aaaacgcaaa gttgatcaaa atatcgattt tgtgtttttt attgagaaat
     7741 tatataggag tgtcaatcga tgatttattg tgaaacagag cgtttaatat taagagactg
     7801 gcatgaagat gatctgttac cttttcaaaa aatgaatgcg aattatgacg tacgtaaata
     7861 ttttccaagt ttattgagtt atcgtcgttc agaattagat atgagaacta tggatgcggt
     7921 tattaaagat tatggcattg gattatttgc tgtagaagat aaagagtccc atcaatggat
     7981 aggctttata ggtttgaatt atattccaga aacaagcgat tatccattta aagaattacc
     8041 gctttatgaa ataggttggc gcttgttgcc agaattttgg ggaaaaggat tagcaactga
     8101 aggcgcaaag gcaacattga agttagcaga agaacatcaa atatacgatg tctatagttt
     8161 tacagcagaa gcaaataaag cttcacaacg tgtaatggaa aaaattggca tgacagtgta
     8221 tgatcatttc gaattaccca atctaagtaa gtatcattta ttaaaaaggc aagtgcgcta
     8281 ttacattaat cttcgaaagt gaaaaattta tacataagcg taacaaacac ccctaacatt
     8341 gtttagctga tgataacaat attaggggtg tttgtttatt ttttaacctt agaatgatta
     8401 atcgtatgaa cgagtaccca gaggtttgaa atttaatatt gattcaatta atgattcctt
     8461 agtgtcgcat aacggtgcaa gagcacgata cttaggatca ataaaacctt cctcaatcat
     8521 atggtcaatc attgtttgta gtggattgaa aaagccatta atattataaa tggcaatagg
     8581 cttttcatgg atacctattt gagcccaact atacatttcg aaaaattctt ctagtgaacc
     8641 tgcgccacca ggagccatga caaatgcatc tgcaagttct gccattttat ttttacgttc
     8701 atgcatagaa tcaactaaaa ttaattcagt taaacgttgg cttgtgattt catgttcatc
     8761 taacatttta ggcatgacgc caatagcttt gccgccatga tctaatacac catcttgaat
     8821 ggcacccata atgccaattg accctgcacc aaatactaat tcataacctt gttcagcaaa
     8881 atatttacct aaatcgtatg ctttttgtac atatgaaggg tcatgacctt tgcttgcacc
     8941 acaataaact gcgattcgtt tcatgttaat ccagctcctt aattcgatga atgactttta
     9001 atagtgattg ttcaaacact ttttgatctt gctttgtaaa aggtggggga cctttgtggc
     9061 gaccaccttg ttttctaatt tgtgcattca tatatcgttt atctaatagt tgttgaatat
     9121 ttttggaatt gtatatcttc ccattatgat gcatgacaat taagactttg tcgactaata
     9181 aacttgcgag tccataatct tgagtgacta cgatatcatc cttcgttgat aattgaacaa
     9241 ttttgtaatc aactgcatct ggtccatcat caacatataa tgttgataca tgtggaggat
     9301 ataattggtt cgaaaaatgg ctgaagctcc gaataattgt cacaaaaatg cctgtctcag
     9361 ttgttaaatc tataatagaa tcaacaacag gacaagcatc tccatcaata ataatatgtg
     9421 tcacaattat gcctctgtat tgttttcttt attttgttga gaggcgcttt tggcaacata
     9481 atctttatat tttttaaatg acttgatgcg tgctttatca gcttcttgtt ggcgtttttg
     9541 ttcttctttg tgtcgttttt caatattttt ttgtaacttt ttattcattt tagcgatttc
     9601 tttgcgattt ttttcagcta gtttatcgcc ttttttctca gttttctcat ctaatttatt
     9661 aggtgttaag cctgcttttt cttcgtattt ttgtgatttt ttcatatctt taatacgttg
     9721 tatttcattc ttttcgcggg ctttttgctc ttctttatga cgcttttcga tatttttttg
     9781 aagtatttta ttcattttat cagcgtcttt acgattttgt ttagctaatt tttcgccttt
     9841 tttctcaata taggcaggat catgttctct agcaaacttt ttaagttcac gtttattttc
     9901 aaaatcttgt tttttatcgc cgacatattc tttaacatca ctcgctgtgt tactgattgc
     9961 tgcagatgtt tttgaagcaa ctttacttgt agcatctgta actttttgta cgtccggatg
    10021 ttgtttgata cgtttacgtt caacaattaa cggtaccaat acaattggta atacattaat
    10081 cataaatttg atgacttttt tcttatccat agatcttgcc tccataatta ctttattaat
    10141 tttacatacc ctatgataca tcaatataaa cgatgatagt agtgaatcac tattaagtat
    10201 ttcagatctt ttttaaaaga agacaataaa aactgccaat caagtggttc cttaattgac
    10261 agtctatatt ttaaaaggaa attaaatacc tttaccaatg ccaaatccga agtaaagtat
    10321 agcaataaag attactaata caattctgta aatggcaaat ggaattaatt tgattttgtt
    10381 aattagatgc aagaatgttt tgattgcaat tagtccaaca gtaaatgcag ctaaaaagcc
    10441 taaaatataa aaaggtatat cagtaatctg aatatcttga taatgtttta ataaagataa
    10501 accactagct gctaacataa ttggaacagc cataataaat gtgaagtccg atgctgcttt
    10561 atgatttaat ttcattaata ccccagttga aattgttgag cctgaacggc tgaaaccagg
    10621 ccacatagct actgcttgag agataccaat tacaaacgct tggaaataat tgatttgatc
    10681 cactgtttgt gggtttttaa ctttagctga gtatttatca gcaataatca tatagatagc
    10741 acctacgaat aagccaatca taacagttgg cacactaaat aaatgttctt cgatgaaatc
    10801 atcaaatagt aagcctaaaa tacctgctgg aaccataccc actaatacat gtaataaatt
    10861 taaacgtctt ggctttgaac gtctttgttg attgttatct ccttcaacat gtttgtgttt
    10921 accaatatgt aaaatctcta agaagcgttc gcggaacacc catgctgctg caaagacgga
    10981 tcctaattgg atgacgattt taaatgtaaa tgctgattga gaacctaaaa attcagatga
    11041 ttttaaccac atatcatcaa ctagaatcat atgtccagta gaggaaacag gtgcaaattc
    11101 tgttaatcct tcgacgaccc ctaagataat accttttatt aattcaatga taaacataat
    11161 gtacccactt tcattactca atttaattta tttaaatatc aaaattacca tatcatgata
    11221 gcatattcat ttaaagacat gctagttata gttataatac tagactaaag atgtatatat
    11281 tcatttt
//
LOCUS       DAMZLE010000084        11250 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMZLE010000084 DAMZLE010000000
VERSION     DAMZLE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.085"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(94..909)
                     /gene="sdhB"
                     /locus_tag="POB92_001857"
     CDS             complement(94..909)
                     /gene="sdhB"
                     /locus_tag="POB92_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDI6579341.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(909..2675)
                     /gene="sdhA"
                     /locus_tag="POB92_001858"
     CDS             complement(909..2675)
                     /gene="sdhA"
                     /locus_tag="POB92_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDI6579342.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(2727..3341)
                     /locus_tag="POB92_001859"
     CDS             complement(2727..3341)
                     /locus_tag="POB92_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDI6579343.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(3665..5446)
                     /gene="uvrC"
                     /locus_tag="POB92_001860"
     CDS             complement(3665..5446)
                     /gene="uvrC"
                     /locus_tag="POB92_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI6579344.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFIDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            5612..5707
                     /locus_tag="POB92_001861"
     CDS             5612..5707
                     /locus_tag="POB92_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579345.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(5770..6084)
                     /gene="trxA"
                     /locus_tag="POB92_001862"
     CDS             complement(5770..6084)
                     /gene="trxA"
                     /locus_tag="POB92_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDI6579346.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(6257..8605)
                     /locus_tag="POB92_001863"
     CDS             complement(6257..8605)
                     /locus_tag="POB92_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDI6579347.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSVKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(8615..10327)
                     /gene="polX"
                     /locus_tag="POB92_001864"
     CDS             complement(8615..10327)
                     /gene="polX"
                     /locus_tag="POB92_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDI6579348.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(10400..10921)
                     /locus_tag="POB92_001865"
     CDS             complement(10400..10921)
                     /locus_tag="POB92_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDI6579349.1"
                     /translation="MVIDFIIIIFFVYFVMVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(10922..11188)
                     /gene="zapA"
                     /locus_tag="POB92_001866"
     CDS             complement(10922..11188)
                     /gene="zapA"
                     /locus_tag="POB92_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI6579350.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         3409 a         2173 c         1581 g         4087 t
ORIGIN      
        1 cttattaaaa aaatcccttc caagttattg tgtatctcca ttcaatttaa ttttgaaagg
       61 aacataacat tttaactcaa aagggattaa tttttattct acttcatggt ctgaaccaaa
      121 gaatgattta aacatgtgga atgttgtttc tctgttcatt gctgcaatgg atgttgttaa
      181 tggaatacct ttagggcaag cattaacaca gttttgtgaa ttaccacact gctgtaagcc
      241 accagtaccc attaatgcat ttaaacgttc atctttagtc atagatcctg ttgggtgcaa
      301 attaaacaaa cgaacttgcg agattgcttg cgcaccaacg aatttattat tttcagtaac
      361 attaggacaa acctctaaac atacaccaca tgtcatacat ttagataatt cataagctgt
      421 ttgacgtttt ttctctggca tacgtggtcc cggacctaaa tcatacgttc catcaattgg
      481 gatccatgct ttcatacgtt ttaagttatc gaacattcta gaacgatcaa cttgtaagtc
      541 acggataact gggaaagtat tcattggctc taaacgaata ggttgttcta attgatcaac
      601 aatcgcagaa caagattgtc ttgcacgacc attgataacc atagaacatg ctccacatac
      661 ttcttctaag cagttcatat cccagacaac aggtgttgtt ttttcacctt taatattaac
      721 tgggttacgt ctaatttcca ttaaacaagc aatgacgttt aaattttcac gatatggaat
      781 ttcaaatgtt tcttcataag gcttagaatc acttgtatct tgtcgtttaa taattaattt
      841 tactgttttt tgtttcggtt tagattgtgt ttcatgttgt ggagtgtttt tcactgattg
      901 ttcagtcatt attttttacc ccctttagac ttacttgtgt aatcacgttt acgaggtggt
      961 attaaactca catcgacgtc atcataagta aactgtggtt tttcaaatgc gccttggaat
     1021 gaggccattg tcgtttttaa ccactcttca tcattacgct ctgggaattc tggtttataa
     1081 tgggcaccgc gtgattcgtt acggttatat gcaccaatcg taataacacg tgcaagtact
     1141 aacatgttcc atagttgacg ggtaaagaat accgcttggt tactccaagt ttgagtatct
     1201 tccatatcaa tatcttcata acgtttcatc aattcaacaa tctttttatc tgtttctaac
     1261 agtttttcat tttcacgaac aacagttaca tttgctgtca taatttcacc aagttcacgg
     1321 tgtaatttat aggcattttc tgtaccgcgc atagctaata atttatcaaa acgttcttgc
     1381 tcttcagctt tacgtttttc aaaaatactt tcgtccatat cagtatatga tcgatcaata
     1441 tttgaaatat aatcaatcgc gtttggacct gctactgtac caccataaat cgctgataac
     1501 aatgaattgg cacctaagcg gttaccacca tgttgagaga agtcacattc tccagctgca
     1561 aataaccctt taatatttgt catttgatca taatctacat atagaccacc cattgaatag
     1621 tgaacagctg ggaaaatctt cattggtact ttgcgtgggt catcaccagt gaatttttca
     1681 taaatctcaa tgataccacc tagttttaca tctaactcat gtggatcttt atgtgacaaa
     1741 tcaagatata ccatgttttc gccatttata cctaattttt ggttaataca tacatcgaaa
     1801 atttcacgcg ttgcgatatc acgaggtact aagttaccat aatcaggata tttctcttct
     1861 aagaagtacc aaggcttacc atctttatat gtccaaattc gtccaccttc accacgtgct
     1921 gattcactca ttagtcgcag tttatcatca ccagggattg cagtaggatg aatttgaatg
     1981 aactcaccat tagcataaat agcgccttgt tggtaaacaa tggaagccgc tgatcctgta
     2041 ttaatcattg agtttgttgt tttaccgaaa ataataccag ggccacccgt tgccataata
     2101 actgcatctg aaccaaatgt ttcaatctca gcagttgtca tattttgtgc aacgatacct
     2161 cttgcactat catcgtcacc tttaactatg ccaaggaatt cccatccttc atacttcgta
     2221 actaatccat ctacttcata tgcacgaact tgttcatcca atgcatataa taattgttgt
     2281 ccagttgttg cccctgcata tgctgttctg tgatgtaatg taccaccgaa acgtctaaaa
     2341 tctaatagac cttcatttgt tctattgaac attacgccca tacggtctaa taaatgaata
     2401 attttaggtg ctgcctctgt catcgcttta acaggtggtt ggtttgcaag gaagtcgcca
     2461 ccatacactg tatcatcaaa gtgaatccaa ggagaatcgc cttccccttt agtattgacc
     2521 gcaccattaa tgccaccttg ggcacaaaca gagtgcgaac gctttactgg tacaactgag
     2581 aacaaatcta catgtgcacc tttttctgcc gctttaattg ttgacattaa gcccgctagg
     2641 ccacctccga caacaataag atgtttctct gccataaaaa tttcactccc ctaaattttc
     2701 aatctatatt tgttaaatgc gatgtattac ataaaggcaa taattgcagt aacaccaata
     2761 tacgaaataa ctaaaaatac gattaatgaa acccatgtaa atactcgttg tgattttgga
     2821 gattgaagtc caccccaagt aactaagaat gaccataagc catttgcaaa gtggaacaca
     2881 acagcaataa tacaaataat ataaaatatt gcccatccag gatgttgcaa tgtttcgtgc
     2941 attaaatcgt aattcacttc tttgccgtaa aatgcttttt gtaaacgtgt ttgccataaa
     3001 tggataccaa taaagataaa tgttaagata ccactcactc tttggaagaa gaacatccag
     3061 tttctaaaaa tcgagtaatg tccaacattt tcttttgctg taaatgcaat gtgtatacca
     3121 aacaaaccgt gatataacaa cggaatgtat ataaataaaa attctacaat aattagaaat
     3181 ggtaatgatt ccataaagtt agatgcctta ttaaacgctt cagcaccttg tgttgcttgg
     3241 tgattcacta ataaatgaac gaccaaaaat gcacctattg ggataatacc taataacgag
     3301 tgaatacgtc ttagataaaa ttcatttttt gattgagcca aaaggagtcc cccctgtgaa
     3361 cgaatattta atttattgag ctatttatat taaacgtacg cttaaccccc taaagtgata
     3421 tcgtttctag cgttgtcatt atcattaagc gaaacatttt aaagacaaat acacactgca
     3481 cgatcaccaa actgcatgtc gaacaatgta acatttggat tcgatatcta aaattgcttg
     3541 tgatgataaa ctttctcatt tagaaaacgc ttccacgtac attcaaaaaa ataactttgt
     3601 taaccatatt gtaacattat ttcatatatt ttggggcatg agaatgattc tcacgcccag
     3661 taatttattt atgcaattgt tcatgtaggt tctttgcgac gttttcagga atacctatat
     3721 ttttaaaatc ttcaagtgta gcttccttca ttttcttgat tgaaccgaat gaacgcaata
     3781 ataatgtttt acgtttgtta ccgataccat ctatatcatc aagtattgat ttcaagcctg
     3841 tcttttgacg tgtttgtcta tgaaatgtga ttgcgaatct gtgaacctca tcttggatac
     3901 ggtgcaacaa ataaaatgcc tggctatttt tcttcagtgg tacaatttct gcactagcgc
     3961 catataataa ttcagatgtt tggtgtttat catttttctg caaacctgca acagggatat
     4021 caagacctaa ttcgttttgt agcacatcaa taaccccgtt catatgtcct ttaccaccat
     4081 ctactattat taaatcaggt aatggtaatc cttcgtttaa aacgcgagaa tatcgtcgtc
     4141 ttactacttc tctcattgat ttgtaatcat ctggaccttt aaccgttttg attttatact
     4201 ttctataatt tttcttatct ggtttaccgt cgataaatgt aaccattgct gacactggat
     4261 ccacaccttg aatattagaa ttatcgaatg cttcaattct aattggtgtt tgaattccca
     4321 tttgtgttcc aagttcttca atagctttaa tcgttcttga ctcatcacgt gatattaatt
     4381 caaatttatt atttaaggat actttagcgt tatgtgcagc taggtcaacc atatcttttt
     4441 tgggacctcg cgcgggttga acgattttag tgtccacaac agattgaatc atttctttat
     4501 ccaaattacg tggtacatga acttccttag gtaaaatatg ttggtttaag ctataaaatt
     4561 gtccaataaa tgtataaaat tcttcttctt ctgtttgctg taatggaatc atcgttgtat
     4621 ctcgctttat catattacct tgtcgtataa agaaaacttg gatacacatc catcctttat
     4681 caacactata accaaagaca tcacgaatcg ttttatctga tgacataatt ttttgtttgt
     4741 ttgtcagatt ttgaatatgt tgaattaaat ctctatattc tttagcccgt tcaaaatcaa
     4801 gtgattcact tgcagttaac attcgctctt ctaaactttt taaaattgtt ttgtcttccc
     4861 cattcagaaa atcagtaatt tccttcgtca tttgtgcgta tttactcaaa tcaacgtcat
     4921 atacacatgg tcctaaacat tgtccaatat ggtaataaag acataattta tctggcatct
     4981 tatcacattt gcgatatgga tatattctgt ctaataactt tttagtttct tgagcagaat
     5041 atgcattcgg atacggtccg aaatatttgc cagtaccttg ttttacagtt ctcgtcacta
     5101 gtagtctagg atatttctcc ttcgtaattt taataaatgg ataactttta tcatccttta
     5161 ataatatatt atatcttggt tgatattgtt taatcagatt caattcaagt aaaagtgact
     5221 ctgtttcact agacgtgaca ataaactcaa agcgacgtat ttcaccaacc agtctcgttg
     5281 ttttagcatc atgagcaccc gtaaaatatg atctcaatcg atttcttagc tttttagctt
     5341 tgccaacata tatcacttga tcattacgat ctttcattaa atagcagcct ggttccatag
     5401 gtacgacatt taatttattt ttaattcgtt gcttatagtc ttccaaaaat atcccccctt
     5461 ctaattttaa aatgaatgat gctacaaaag tgcttcaaat ttatcaccac taattctagt
     5521 taataatcaa catcttcgag tatgactttt catgcacatc atgtaatatt gaacattgtt
     5581 ttatcaaacc aactgctaca aatctatctg catgctagat tatcatttaa cgttcataga
     5641 tttattaatc ccaccattct caagtcgtaa acaatatgat gacttatcat tcaacttttt
     5701 atattaaaaa cacaaacaaa aactggagca attcattaaa gaaatgcccc agtcgtcatt
     5761 ggttgtaact tataaatgtt tatctaaaac ttcagctaag ttttcttttg gttggaaacc
     5821 aacaacttta tcaactggtt gaccgtcttt aaagacgatt aatgttggaa tactcatcac
     5881 ttcatattta gctgcagttg atggattttc atcaacatct aattttaaaa tgtcagcttt
     5941 accttcatag tcagctgcta attcttctaa taccggagcg atcattttac atggaccaca
     6001 ccatgttgcc caaaaatcta ctagttgtac accagattct acttttgaat caaaatctgc
     6061 atctgttact tttacgattg ccataaatgc caatcctcct taattattat tatgatgttc
     6121 aaattatatc agataacatg cttcatgtat ttctatttgc tcaattttta tgcttatttc
     6181 aaataacatg tttgaaatgt attcttgttg ctttacatta ttttaactgc agctatttaa
     6241 tttatcaaat tataatttat tttagtgttg caacggtaac gccaaatcca ccttcgcttg
     6301 gcataccacc tctaaagtca ctaacacttt tatgcttttt caaatgttgt tgtacacctt
     6361 tttgaagtgc acctgtacct ttaccatgaa tgatataaac ttgttcgtaa ttacttaaaa
     6421 cggcttgatc taaatattga tctagttcaa ttaaagcatc ctcataacga tagcctcgta
     6481 agtcaagttc agttttaatt gtttgacgat tttgacgtgt aaccattttc gttggcttaa
     6541 ctttttcttt ttgttttttc tctaaatctt caataggtaa cttcatttta ataattccca
     6601 tttgaacaat tgcttcttca tcattgacaa tttctaaaac ttcacccttt tgaccgtaag
     6661 ataatacttt tacttcatca ccagcaacaa ttttatcgta tttttgcttt tgtacatttt
     6721 gctttataga tttcgcttca taatgatcat ctaatcgttt cttcttatca atcaattcat
     6781 gttctttaac atctgcacct ttttgttcac gcaattgtct taagtcttta ataatatcgt
     6841 cagcttcttt tgttgcagcc ttaatcttct gatttgcttt ttctttcgct tcctctatta
     6901 gagacttttc ataattttgg aattgttgat actgcttaga taaatcatca tgcacttgct
     6961 ccgcttcttt tacaagacgg tccagttcta acctctgtgt ctctacacgt ttgtaattac
     7021 gctctaatga ttcaatcatt tcatttattt ctttttcatc agtaccaatc atcgtcttag
     7081 ccttattaat aatattcaaa ctaagaccta actttttaga aatgtcaaaa gcatttgaac
     7141 gacccggcac acccattaat aacttatacg ttggactcaa agtatctaca tcaaattcta
     7201 cactcgcatt cataacgcct tctcgattat aactatatgc tttaagttca ggatagtgcg
     7261 tcgttgccat tactagagaa ccaatttttc taacatgatc taaaatgctc attgctaatg
     7321 cagcaccttc actcggatct gtacctgcac ctaattcatc aaataaaact aaactatgtt
     7381 tgtctgcatg ctttaaaatt tcaactatat tcgtcatatg agatgaaaaa gttgataatg
     7441 attgttctat tgattgttca tctccgatat cgcaatatac atttttaaat acacttaact
     7501 gactaccatc aagtgtggga atcaacaatc ctgattgagc cataacaata attaaaccta
     7561 atgtttttaa tgttacagtt ttaccacctg tattcggtcc tgtaataatt accgtttcaa
     7621 tatcttccat aaattcgatg gtattagcta caacagtctc acgatttaat aatggatggt
     7681 atgctttagg taaatataca gtacggtcct ctttaaatat cggctttgtt cctttaacac
     7741 ttctactata tctcgctttt gcgattaaaa aatctaactg acccatgact tgttctgcca
     7801 caagtagtgc atctttgtcc gcagccacat aaccagttag ttgcgttaaa atgcgttctt
     7861 tttcaattgc ttcgtcatga cgtaatcgac taatttgatt attcatttca acaactgatg
     7921 atggctcaat atacaatgtt tgtcctgaag cagattgatc atgtacaatc ccattaaaat
     7981 cttgtcgata ttcagctttg acaggtataa cgtttctttc attcctaact gttacaatag
     8041 catctgataa ttttttctga tttgcttggc ttttaacaat acggtccaaa ttttgtctaa
     8101 tacgttgatt cgtgctagaa attttacttc taatcccttg caattcataa ctcgcattat
     8161 catataaatc atacgtatcg catgtttcat ttatttgatg aaaaagatca gttaacacag
     8221 gtaattgatt catcttgtca tctaatattg ggtatttaac accttcatct tcttcaacca
     8281 attgattata aaatgtcttg aattgatttt gtacttgaat taatcttttt atcaagttaa
     8341 gctctgatac atttaaaacg ccgccaatat cagcgcgatg aatgaatgct gatactttag
     8401 ataagccact caagcttggt aaacgatgct tattataaat ttgagcaatc tcatccgttt
     8461 cttccatttg aaaaacaacc gtttcaaaat tagtagctgg catcatttga ttgacctttt
     8521 ccaagcctaa gtcactaata gtttcattgg caacgagtga ttttattttt tcaaattcta
     8581 agacgtctaa tgttttttgt ctcataaaat ccctctattt cttaagtttt gtattatttt
     8641 caataaagtc tttaaaagct tcacgcgata atgcgttaat cactctatct tttgttacaa
     8701 atcctttttg cgcagttgct acgccataat tcataaaatc taaatgattt gtatgatgcg
     8761 catcagtgtt aatagttaat ttcacatttg gatatttacg aacgatatca gcgctcagat
     8821 ccagtcgatg tggattggca ttaatttcta atactgtatt cgtttcttca gctaatgcca
     8881 ttaattgttc aatattcggt ttataaccat ctcttctacc tataatacgc cctgttggat
     8941 gcgctatatg tcgcacgtat ggattgcgac atgcattagc taatcgttcc ataatttgtt
     9001 cttctgattg gttaaagctt tgatgaatag ctccaattac ataatcaagt tgtgctaaaa
     9061 tttcatcatc ataatccagc gagccatcag gtaatatatc catttctgta cctgaataaa
     9121 tatcaatttc actatattct ttatctaaag ccttaatttc ttcgttttgt cttaaaagtc
     9181 tttctacttg taagccatta gcaacacgta aactttgtga atgatcagta attaccatga
     9241 atttataacc ttttgcgata tttgcttcta ccatgtctcg aatagaaaac gcaccatcac
     9301 tatacgttgt atgcatatga atatcaccat taatatcatc tattgtaatg atattactta
     9361 gatctttatc aaattcgcta ccatcttctc gcatagcagg tggtataaaa ttcacattaa
     9421 aatgttcata tatcttggct tcactatcat attgaattaa tgtaccatca gcttgttcaa
     9481 ttccatattc acttactttt tcatcacgtg ctttagcaag ttgtcgaatt cttatattat
     9541 gttcttttga cccagtaaaa tgctgcaatg tatgataaaa agcacttggt tcaattaatc
     9601 gaaaatcgac accaatcgtt tcatcatcat acgctaattc taatgaaact tttgtgttcc
     9661 ccactgcaac ttcttttact ttattgggaa tatttaataa ttgctgctgc actgcttttg
     9721 ggttatcggt acttattatg aaatctaaat ctttgctcat ttctttaaaa cgacggaagc
     9781 ttcctgcaga tgaatattga tcgatataat ttaatgtatc tatataatca atgatttctt
     9841 gattaagtct tctcatttga tcaattggat atctatcttt cttagcacca agttgtttca
     9901 cagcttctaa tatgttttgt tccgttttct tagcaaatcc gcttaattca ctaacttttc
     9961 cattttcaca agcaacttga agtgacgctt tatcaacaat attcaactct ttatatagct
    10021 tagcaatttt cttgcttcca agtccttgaa ttttcaaaag tggaataaga ccttccggaa
    10081 cttcttcctg taattgctgt aaatactgag attcaccggt ctcacggtaa tcattgatta
    10141 cttctgcaac acctttacca atgcctttta actccgttac atcagatatt tcatctaatg
    10201 gtcgttcatc taattcaaga ctttgagctg cttttcgata cgctgatatt ttaaaagtat
    10261 tttccccttt taattccata taagtagcaa tttgttctaa tagtttgata acatcttttt
    10321 ttgtcataat aacactccat aaaaagaaga ccaggacgta tcattaatat atacctttgt
    10381 cctgacctct tatgataatt tataaattaa tggtgaaagc cgataaatat gggatgtgaa
    10441 aaatcaatga atgactcaca atagatattt taagctgatg ttgtaatgct tcgttaggat
    10501 ataatgccaa tagatataac gtaaagtgta agacaattat cgtcataaac acaccaacta
    10561 tcattcccat tgcacgactg aaaatatgaa tgttttgata cgctattatt ttatcaaaag
    10621 ttacgataat tagatataaa atgaacttac aaaacaatgt aatcattaaa aaagctacaa
    10681 tcgcttcaaa tcgattttgt agatgattaa aatgaaacgc aaaagttgta ttaaatgctg
    10741 ttgttttagg atatggaata aatacaatta atctttctac aatagatttg taaaattgac
    10801 tggcaatcca caatgataca atcgttgcac tcaaatgtat catagataac caaaaacctc
    10861 gtctgaatcc aaccatgaca aaatacacaa agaaaatgat tatgataaaa tcaatgacca
    10921 tttattgctc acgctgctgc aatttgtgaa tttgttgttt caaacgtcga ttttcttctt
    10981 ctagtagtac tttttcatgc ataatattca cagcagttag tattgctttt cttgaagtat
    11041 ctaaacctgc tgctttatac cctaattctt ttattttatc atcaactaaa tgtgctacat
    11101 atcgtatgtg ctctgggtta tcttccccaa caattgtaaa aagctgatca ttaattgata
    11161 catttacctt gtttttaaac tgtgccattt ataatttctc ctgatccttt ttttaaaatc
    11221 taaattcacg ttataaaata tgactggata
//
LOCUS       DAMZLE010000085        10977 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMZLE010000085 DAMZLE010000000
VERSION     DAMZLE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.086"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..334
                     /locus_tag="POB92_001867"
     CDS             <1..334
                     /locus_tag="POB92_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6579351.1"
                     /translation="NNTLNQKKINKSNNVKFPVTINKFENIVSNEFVFYNASKITIND
                     LSIKLKSAIANDQGITKHDIELAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIK
                     KVDIELKF"
     gene            complement(658..1944)
                     /locus_tag="POB92_001868"
     CDS             complement(658..1944)
                     /locus_tag="POB92_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6579352.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPDHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2144..2242
                     /locus_tag="POB92_001869"
     CDS             2144..2242
                     /locus_tag="POB92_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579353.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2485..2661
                     /locus_tag="POB92_001870"
     CDS             2485..2661
                     /locus_tag="POB92_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579354.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            2920..3300
                     /locus_tag="POB92_001871"
     CDS             2920..3300
                     /locus_tag="POB92_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6579355.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            3297..4193
                     /gene="pmtA"
                     /locus_tag="POB92_001872"
     CDS             3297..4193
                     /gene="pmtA"
                     /locus_tag="POB92_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDI6579356.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNIITPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            4194..4874
                     /gene="pmtB"
                     /locus_tag="POB92_001873"
     CDS             4194..4874
                     /gene="pmtB"
                     /locus_tag="POB92_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDI6579357.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFLLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            4871..5743
                     /gene="pmtC"
                     /locus_tag="POB92_001874"
     CDS             4871..5743
                     /gene="pmtC"
                     /locus_tag="POB92_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDI6579358.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            5743..6483
                     /gene="pmtD"
                     /locus_tag="POB92_001875"
     CDS             5743..6483
                     /gene="pmtD"
                     /locus_tag="POB92_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDI6579359.1"
                     /translation="MRILNLVKYDLYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(6549..7112)
                     /locus_tag="POB92_001876"
     CDS             complement(6549..7112)
                     /locus_tag="POB92_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6579360.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            7233..7757
                     /locus_tag="POB92_001877"
     CDS             7233..7757
                     /locus_tag="POB92_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579361.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGCKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            7817..8374
                     /locus_tag="POB92_001878"
     CDS             7817..8374
                     /locus_tag="POB92_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDI6579362.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            8371..9213
                     /locus_tag="POB92_001879"
     CDS             8371..9213
                     /locus_tag="POB92_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDI6579363.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(9270..10310)
                     /locus_tag="POB92_001880"
     CDS             complement(9270..10310)
                     /locus_tag="POB92_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDI6579364.1"
                     /translation="MQQVISDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            10761..10934
                     /locus_tag="POB92_001881"
     CDS             10761..10934
                     /locus_tag="POB92_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579365.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         3804 a         1419 c         1842 g         3912 t
ORIGIN      
        1 aaacaataca ctaaatcaaa aaaaaataaa taaatctaat aatgtgaaat tcccagtaac
       61 aataaataaa tttgaaaaca tagtttcaaa tgaatttgtg ttctataatg caagcaaaat
      121 tacaattaat gatttaagta taaaacttaa atcagcaatt gcaaatgatc aagggataac
      181 taaacatgac atagaacttg ctgaacgcgc agtgtataaa gtgtatttta aaaatggttc
      241 gtcaaaatat gtagacttaa aaactgagta taaagatgaa agagtattta aagcaactga
      301 cattaaaaag gtagatattg aacttaaatt ctaatcttta attttataat aaggcatctc
      361 acaatcgtgg ggtgcctttt acatttgtag agatgtgatg cttgaatgtt tgaagtgatt
      421 tgccaaaagt tatataaatt ggttctataa taaatcggac tgatagaaaa agttttttac
      481 ttttcatctg tccgattttt tgattttgtt aggagagatg tataaaacta attaggtata
      541 gtgtttgaca agttgctgaa taaaatatat cttatgatac tttgaaagaa ggaataattt
      601 taaaaataaa aaaccaataa tccgagtcat actcatcaga ttattggttg aaattaatta
      661 tcttaagtca tcaatcgctt tagcaattga atcaaataca tgtgggatat cttctttttc
      721 tacacagcta aacgcaatac gaatatctgt tgcattaagt gcaataacac caattgaata
      781 tttttcaatt aaatgtttac gaagttcttc agggtcaaca tcatgaactt ttattgccat
      841 aaagtatcca gagttaaagt cataggcttg ccaatgtgaa tgatattgat cagcatagac
      901 aacttcctta gtgacttcat agcgttcttt taatgtttga atattttgtt cgatttcttt
      961 atcgaattgt ttattatttt ttaatacatg cttaacagcg ctttgtgttg gaagaggtcc
     1021 actagaaatg ttacttcgta taagaccttt tactttggct tctaatactt ctttagttgt
     1081 ttgatcagac gttccaaatg tcataaaccc aacacggaat ccccatgcga aaaattcttt
     1141 tgttgcacca tctaaacgaa caggtaatat tgcatttgaa tttaaattag ataatgcagt
     1201 aaataatgat tgagtataca catcttcata gaataaacca tagtatgcgt catccacaac
     1261 agctataact tttgtacctt tatcagctaa tgctttaatt gcatcgacga tggtagtcac
     1321 ttctttatgc gtaggtgtgt aacctgtcgg attattagga taatttaaaa tcataatgac
     1381 tttatcttta ttgtatgatt gtaaagcttc tacaagtgaa tcagtagtat aatgcccgtc
     1441 tttatcaaag ataggatatg tttgaaggtt tgcaccattt ctcgtattga aaacaagttt
     1501 gtaattaccc caattatgat ctggtaacaa gatagtgtca ccttgattta caaataaatc
     1561 gccaactaaa gataaaccat gtgttaatgc attcgtaaca attggtagtg acatgttgtc
     1621 gattgatagc tctggattgt cacgcaacat tttttgttgc cataaatcac gtaattcttc
     1681 gatgccttgt ggtggcgcat aagggaatat ttcatctgga gttaaatcat taaacattgc
     1741 atctaaagat gatgcaaaca tttttccgtc tttgtttgtc gccataccta tagttgcatt
     1801 atatgttgta ctctttgctt cagcagattg agataaaatg ccttttggat aaaacatatt
     1861 ttgacctaag tcagataaca ttgcaaaggc agttgcattt gactgttgaa gttgttcatt
     1921 taagctttgg gctaaaggat tcattattgc ttaacctctc tttaaaaaaa ttgtatgcct
     1981 atatattatc ttcaattata ccaaagataa agtgttgttg gtgaattaac gaataatatg
     2041 taaaatctgc tgccaaaata cgaacatatg ttctgttttt atgttagaat aggtgtaggt
     2101 catccttcag tatgatactt tagtcgaacg gaggtgtagt gtaatgatgt ggtggcaaga
     2161 agggatgatg acacttatta caggtggctt actaattatt tttcgacttt ggttagaact
     2221 gaaatggaaa aataaaaaat gatcctacac acataaataa ccccaagtct gctggtacca
     2281 gacttggggt tgtgtagtat aattctgtgg atacacttaa ataatatcat tgaacacaat
     2341 atatcgcaat caataatcat tttgattgaa tggttttgtt gcgctatttt agttgaaagt
     2401 gattagtacc ttgaactttt gaaattcaat aacttattaa aaaaatttat atttgttata
     2461 atgtcgatgt tgaggtgaaa taaattgaaa catctaacaa aaatatttgt agtacttgca
     2521 attattttat ttattattgg ttattattta caagcaacta atcatgaaag ccaaggtata
     2581 aaattattat tagcagcgat tatgtttatg atttgtgctt ttataagtag aagtaatgat
     2641 cgacgtaaaa atagtaaata gaattgactg tttaaaacga ttgtttatgt tgacgctacg
     2701 gttgtcacgt agagattaaa gacattcgtt ttagcagtct tttttattga agtagacaat
     2761 gcaagattaa atggtagtgt catttcattt tattcaactg ctagtaatac aatactcagt
     2821 gttatttctg aagactggtt gataatgaga catgcatatt tacaaaactg tgtatattgt
     2881 gtatattgta tatatacaga aagtgatagg gggacgttga tgaaaataat tttaaaaaac
     2941 aatagtgatt ttccgattta tgaacagatt aagcaacaag taaaacaaaa tattttaaag
     3001 ggacatgttg ctcctggaga gcatttgccg tcaatgagag aacttgccaa agatcttcaa
     3061 gtaagtttga ttactaccaa acgtgcttat gaagatttag agaaagacgg ttttgttaca
     3121 acaattagag gaaaagggac ctttgttaag gagcaagata gttctatttt aaaagagaaa
     3181 caatttttta ccattgaaaa tttggttaaa gaattggtta atgaagcgca agccatcgaa
     3241 atgtcacttg aggaacttca agatatttta acgttcattt atgaggagga atcatcatga
     3301 atgccataga attaagtaat gttaattatt caagtgatca gtttaattta aagaatatct
     3361 ctttcaaagt tcctcaagga ttcgtcactg gttttattgg tagaaatggc gctggtaaaa
     3421 ccacaataat aaggttaatt atggatttgt atcaaccaca aactggtgtt attagagtat
     3481 tagaagagga tatggctctc aatccgatag agttaaagaa tagaattggg tttgtttact
     3541 ctgaaaatta ttttaatgag agatggacta cgaagcaact tgaaaaaatg attgcccctt
     3601 tttatcgtaa atgggaccat caagtatttg aattttatct tgaaaagttc gatttaccaa
     3661 tcaataaatc aataaaaaca ttttcgactg ggatgaaaat gaaattatca ctagcagttg
     3721 cattttcaca tcatgctgaa ttgtatattt ttgatgagcc tacttcaggt cttgatccct
     3781 tagctagaaa tgagctattg gagataattc agcaagaatt aattgatgaa aataaaacaa
     3841 tattcatgtc gacacacatt atctcagatt tagaaaaaat agctgattat atcattcact
     3901 taagtgacgg tgaagttatc ttaaatggtt caaaagaaca actgttacaa agatatcaag
     3961 ttgtgagtgg tgctattgag gaccttgatg atgaattagc atctctactt atttatgaag
     4021 aacataagcg tacgggattt ataggattaa cagaacatgc gcaagtattt aaagaaattt
     4081 taggtcataa agtgaatatc attacacctt ctattgagaa cttaatggtt tatcttgaaa
     4141 agagaaaacc taaatatcat gaaaacatca aattgatgga agaaggtttt taaatgaagg
     4201 cattgttaat taggaatttt aaattacgac gatatacact tatcatctat gttctattat
     4261 taaccttata tcctttttat ataatgcttg actcaacaaa gttcttttac ctattgcaat
     4321 cttttataag tccaacaata ttgattattt ggattttaga tgctggtcac ttatttagac
     4381 tcaatcgtag attaggtggt aatgattcat attattttta tatgagttta ccagtgtcaa
     4441 aaaaacaatt gcttaatgcc aactatatta catgcattgt tttaacatta attggaacgc
     4501 ttgttattag tttatatgct tatgaagcag atgtgattga accaaattca atctattttt
     4561 caactgcata tgcatttgtc atatctaact tcttgtctat accaattgca tttagtcaat
     4621 ttacagaatt gcgtagagtc aaagtgccat atggtatata cgtgtttact attatcattt
     4681 tagttccatt tttattttca attgcaatag tgttggtgaa ttattttctt ctaagtcaat
     4741 catcattccc agatttatat tcatatattt taaatattgg ttttctaatt ataagcattg
     4801 ttatacttat tgttaattat tttaaacagc tcaataaaat aaatactaga aaatttaaag
     4861 gaggcagtcg atgaaattag aacatattac aaaaaaatac ggctcaaatg tcgttttaaa
     4921 tgatattgat tttgactttg gcgatagtag aattgtcgga ttaataggaa aaaacggtgt
     4981 tggtaaaaca accgttatga aagtaatgaa tggtaatatt attaaatttg atggaaaagt
     5041 agacattgat aatgcagata atatcggttt tttaattgag catcctaaat tatatgataa
     5101 taaatcagga ttgtataact tgaaattatt tgcacaagta ttaggtaagg gttttgataa
     5161 agcatacaca gacaaaatta tagatgcatt tggtatgaga ccttatatta aaaagaaagt
     5221 taagaaatat tcaatgggga tgaagcaaaa gttagcaatt gcagtatctt taatgaataa
     5281 acctaaattt ttaatcttgg atgagcctac aaatggtatg gatccagatg gctcaattga
     5341 tgtgctgact acaattaagt ctctagtaaa tgaacttgat atgagaattc taatatcaag
     5401 tcataagtta gaagatattg aattaatttg tgatagagct gtatttttaa gagacggaca
     5461 ttttgttcaa gatgtaaaca tggaggaagg tgttgcatct gacacaacga tagttactgt
     5521 tgatcataaa gactttgata gaactgaaaa atatcttgca gagcatttcc aattacaaaa
     5581 tgtcgacaaa gcagacggac atttaatgat taatgcacaa aaaaattatc aagttatact
     5641 aaaagcatta tctgaattag atatttatcc gaaatatatt gaaacacgta aaagttcatt
     5701 gcgtgatacg tacttcaata taaatcaaag aggtgataaa taatgagaat tttaaattta
     5761 gttaagtatg atctttatag tatttttaaa agtcctttaa catatttagc gatactagtc
     5821 gtatctagtt tgattgcaac tcaaagtata cttatggcaa attctatgga taacccgaaa
     5881 catattattg tctatggatc tgtatttgct gcagcaaaat ggttattgtt aataattgga
     5941 ttaatgtttg ttgttaagac aattacgcgt gatttttcac aaggtacaat tcaactatat
     6001 atgagtaaag ttaaaacacg cgttggatac attatttcga aaacaatttc aattatttta
     6061 atttcaatat tatttgcatt aattcattat gtgattttga ttgttgtgca ggcatctagt
     6121 aatggaaaaa atttggcgtt ttctaaatat gtagataatt tatggttctt cctaatcttt
     6181 ttactattct ttggcttgtt tttattctta atcacacttg catcacaaaa aacagcaatg
     6241 atattttcat taggtgtatt tttagtactt attgtaccgt ttattaaacc ttttattaca
     6301 tttatcccaa gatacggtga aaaagtttta gatgcttttg attatatccc ttttgcttac
     6361 ttaactgata aaatgattag ctctaacttt gattttagca attggcaatg ggtaatttca
     6421 ttaggttcta tagtaatatt cttcattttg aatatcttat atgtcgctaa aaaagacatt
     6481 taataaaaat aattttgagg ttgggaattt taaattttcc caacctcaaa gtttgtctta
     6541 ttgtaaattt atttattttc taatttattt aggatggaat tataaactgc tttccaaaat
     6601 gaagcgtcag ttttatagcg gtttgatata actaagtgtg tttctttttc taaatctgca
     6661 tagtctggat gatctttgct cggtaattta tcagcacgaa catcagttac aaatttttgg
     6721 acttcatttg ctcttggtcc ccaaactgtt tcttgttcga attgatcatt caagaatacg
     6781 aagataggaa ttgcacgtga tttaccattt gttaaatatt gatcgatcag ttttgtatca
     6841 tcatctctat ggaacacgcg tacttctaaa tttaatgctt cactgatgtg ttttagaatt
     6901 gggagattca tcattgcatc tccacaccag tcttcagtaa ttactaatac tttagaataa
     6961 ttcatctctt ttattttttt gatgcgtgaa tcatcttctg gtaactcaaa tgattgatag
     7021 atactgagaa cggtatcttg atttgtcttc attccatcaa tgtattcatt taagggttgg
     7081 ctatttttga aataagtttc taaatttgtc attgtaaaaa cctcctttag catttacaac
     7141 attataccaa tttatagtaa taaaaggtaa tgaaataaat taattgcaaa ttctttgttg
     7201 atttttgtta aggatgaaac gggaagcacc ttatgctata tttaaataag tacaaagaaa
     7261 gggtgacatc agtgcgtatt caaaatcgct gggttgtgtt tatattattt ttaatctgtt
     7321 cttttggtgt attaattggt ttatatcaat atcgtcatac gaaaactgtg gatttgtcta
     7381 atcttgaaat aaacgatatt aaattaaatg agaagtttga taaaaaaggc tatgaagtaa
     7441 ataaaaaaat taagtttgat cgttttaaat tttataacag taaagctcac cctgatctta
     7501 ccgttaaagt gagagaaaag gataacatcg ttaaggggat aatattagta agagatgaaa
     7561 agatacatac taattttgat gggggaattg gttcgccgat aaataacgcg attgaaaatc
     7621 ttggattcgg atgtaaaaga acaaaagttg gcaatgattt ctcatcggta aagtatattg
     7681 atagagataa ccatttaaaa ttaaacttac tttatcaaga tttagaaatt aaacgtattg
     7741 aattttttag taaatagctt taggtcttaa agttataaaa aacgaatgaa taattttatt
     7801 gggatgagtg acctatatga acaagattac ttatttaaat gaattagaaa cagcacttga
     7861 tgcgctacct aggcatttaa gagatcaaaa aatgtatgaa tatgaacgtt atttttatga
     7921 gcaagaactt aatggcgttg atgaagggga aattttaaag aagttaaaag acccacaaga
     7981 tgttgcagct gaaacaaaag ctagaagtgt tattgattat gctgaatcta aaccaacatt
     8041 tgaaaatatt tcaagagctg ttgctgcttc attaagttta ggcattctat ctatttttgt
     8101 catccttata ccagtatcta tagttggatt atttgtatta gcattatttt taatatcact
     8161 tttgctgctg ttttgtccaa ttattttatt agcatcagca atatccagag gaattgtgga
     8221 ctcaattagt aatgtatttt ttgccatatc atattcagga ttaggattag tatttatcat
     8281 tgtcatattt aagattttag aatacattta tcgtttaatc ttaaaatatt tactttggta
     8341 tattaaaact gtcaaaggaa gcgttagaaa atgaagaaat tcttttttat tgggctttta
     8401 gtgtttgttg tcttttttac agcagcaacc attatttggt tcagctatga taaaaacaaa
     8461 tatggtacta aacaatatga taaaacattc aaagacgatg cttttgacaa tgtatctata
     8521 aatttggata gtacagaact tcgtataaaa cgggggaatc aatttagagt taaatatgat
     8581 ggtgacaatg atatattaat taatatagta gataagacgt tgaagattag tgataaaagg
     8641 tctaagacaa gaggatatgc aattgatatg aatccttttc atgagaataa gaaaacgtta
     8701 acgattgaaa tgcctgataa aatgattaaa cgtttaaatc tatcatctgg agcaggaagt
     8761 gttagaatca gtgatgttga tttagagaac acaagtattc aaagcattaa cggtgaagta
     8821 gttattaaaa actcaaatct tgacgcacta gattcaaaga caaataatag ttctacttat
     8881 attagtaaaa gtaacattaa aaatagcaat attaaagttg ttattggtac gctacaaatc
     8941 gacaagagtc aaattaaaca atccatattt ttaaacgatc atggtgacat tgaatttaaa
     9001 aacatgccat caaaagtaga tgcaaaagct tctactaaac aaggagatat tcgttttaag
     9061 tatgatagta aacctgaaga cactatacta aagctaaatc cgggaacggg tgatagcgta
     9121 gttaaaaata aaacatttac taatggtaaa gttgggaaaa gcgacaatgt tttagaattt
     9181 tatacgattg atggtaatat caaagttgaa taaataaagg atgtaagcac cgatattagg
     9241 aagcataatt tctctaatat cggtgttatt tatttgttgg caaaagttaa gtcggtatct
     9301 atattgccag taaagtgagt gatattaagg tcttgaccat ctaaccatga tttgaaatct
     9361 attatttctg gtggcgcatt ttctcccaat gtaaaatatg cagttaatgt ttcaggttga
     9421 tacattgatg tatggatggt gccagaccag cttttgaata gtttactgta aatttcatac
     9481 tgaggattat tgaataactt aaatgctgta gtcatatcta aattatcatt agtttgtgaa
     9541 atggtacgcg ccagtctttc tttagattct tttgtataat tacgattttc atgtgttaat
     9601 atttcaaaat gatttgtaca tatattatca taacgaacat ctattgatct cggtgtcact
     9661 tcaacaattg catggttcaa tgatttgtcc atcagtatgt agctaaatga gcttctgtgt
     9721 ggtatttctt tcaataattg tattgcttct gttacatttc ggcaattttc aagaattaga
     9781 cgaccaatca tataacatac aaaaccattt gctggttttt tccggtgcat aaagttatag
     9841 cccatagtta atcctgactc attcatacca tccattcttc cagttaccct tgatacagga
     9901 ccaatttgag ctaaaccgct atctgtaggt tgatatagta agtagcgacc atcataagtt
     9961 gcagggtggt aatcataatt tctaaccatg aagtctttgc cttgaaagac cgtgcaacca
    10021 ctttctttta aatcggtaaa acgataatgt ccaaagttta aaataatttg gcgtgttggc
    10081 attttgagta tactttgtag tcccattaat tcttcccata tttgaggtgc gtaagtttgg
    10141 aatatttgat aagtttcatt tacatctata tcgaaacgtg ggacacgttt tttccattct
    10201 ttttctcgat tttttagaag aggtgtttgt tgaagccatt taccagtttt aacacctaac
    10261 tcgaaatgtg aacctctaaa agtcataata tctgatatca cttgttgcat atcatcggcc
    10321 cctttctttt tagttgtaat atattgtaaa taaatagtaa tcgtatgtat attgaatgtc
    10381 atgttaaata aagttatatt ttactaaatg aaatataaaa ttgtttgagg tgatttctcg
    10441 gtgtataaga cttatcaatc agttaaaaca tatttttata gatggtgggg atattaagtt
    10501 aaaaacttaa aatcatctta tcataaataa caatcttaag ttagcattca cgataatagt
    10561 cattgttaac attagcatat aaggtcatgt cacgttgaaa cagaggttcc tcggcatttt
    10621 tgaaaataat cattagcttg ctcactatat aatttgatga atatatttcg taaaagtggg
    10681 tatttattta atgattattc tatatatgat agtataatga aatgtagata ggtatttaat
    10741 ttaacagagg tgaaattgag atgtggaatt ttattaaatg tgtgtttaaa ttcgtattta
    10801 gcttagttgc tattacaaca ttagttgctg gtgttggtgt agtagcattt gcttatatct
    10861 ttaaaaaaga ttttgaagat attgaaagaa aaactaaaga aattatttct gatattgaaa
    10921 gtaaaaataa ctaataacat ttagaggctg ggacataaat ccctaaaaaa cagcagt
//
LOCUS       DAMZLE010000086        10960 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMZLE010000086 DAMZLE010000000
VERSION     DAMZLE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.087"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            296..1984
                     /gene="ilvD"
                     /locus_tag="POB92_001882"
     CDS             296..1984
                     /gene="ilvD"
                     /locus_tag="POB92_001882"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDI6579366.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            2012..3781
                     /gene="ilvB"
                     /locus_tag="POB92_001883"
     CDS             2012..3781
                     /gene="ilvB"
                     /locus_tag="POB92_001883"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDI6579367.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLELEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3781..4035
                     /locus_tag="POB92_001884"
     CDS             3781..4035
                     /locus_tag="POB92_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6579368.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4172..5176
                     /gene="ilvC"
                     /locus_tag="POB92_001885"
     CDS             4172..5176
                     /gene="ilvC"
                     /locus_tag="POB92_001885"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDI6579369.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQDASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGTENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5206..6735
                     /locus_tag="POB92_001886"
     CDS             5206..6735
                     /locus_tag="POB92_001886"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6579370.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYGIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6738..7784
                     /gene="leuB"
                     /locus_tag="POB92_001887"
     CDS             6738..7784
                     /gene="leuB"
                     /locus_tag="POB92_001887"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDI6579371.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7798..9168
                     /gene="leuC"
                     /locus_tag="POB92_001888"
     CDS             7798..9168
                     /gene="leuC"
                     /locus_tag="POB92_001888"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDI6579372.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFVKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEISDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9169..9741
                     /gene="leuD"
                     /locus_tag="POB92_001889"
     CDS             9169..9741
                     /gene="leuD"
                     /locus_tag="POB92_001889"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDI6579373.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9756..>10960
                     /gene="ilvA"
                     /locus_tag="POB92_001890"
     CDS             9756..>10960
                     /gene="ilvA"
                     /locus_tag="POB92_001890"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDI6579374.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKTDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRV"
BASE COUNT         3712 a         1803 c         2135 g         3310 t
ORIGIN      
        1 tagataattc taactattaa agtgatatat accattcacg gaaggagtat aataaaatgc
       61 ttaatcaata tactgaacat caaccgacaa cttcaaatat tattatttta ttatactctt
      121 taggactcga acgttagtaa atatttacta aacgctttaa gtcctatttc tgtttgaatg
      181 ggacttgtaa acgtcccaat attattggga cgttttttta tgttttatct ttcaattact
      241 tatttttatt actataaaac atgattaatc attaaaattt acgggggaat ttactatgcg
      301 aagcgacatg atcaaaaaag gagatcacca agcaccagca agaagtcttt tacatgccac
      361 gggcgcgcta aaaagtccaa ctgatatgaa caaaccattt gtagctattt gtaactctta
      421 tattgatatt gttcctggac atgttcactt gagagagctt gcagatatag ctaaagaagc
      481 aattagagaa gccggtgcca ttccatttga attcaataca attggtgttg atgatggaat
      541 agctatggga catatcggaa tgcgatattc tctaccatca cgtgaaatta ttgcagatgc
      601 agctgaaact gtaattaacg ctcattggtt tgacggcgta ttttacattc ctaattgtga
      661 caagattaca cccggtatga ttttagcagc catgaggaca aacgtaccag ctatcttttg
      721 ctctggtgga ccaatgaaag ctggcttatc tgcacatgga aaagcattaa cactttcatc
      781 aatgtttgaa gcagtcggcg catttaaaga aggatcgatt tctaaagaag aatttttaga
      841 tatggaacaa aatgcctgcc ctacttgtgg ttcatgtgct gggatgttta ctgcaaattc
      901 aatgaactgt ttgatggaag ttttaggtct agcattacca tacaacggta ctgcacttgc
      961 agtcagtgat cagcgacgag aaatgattcg ccaagcagct tttaaattag ttgaaaatat
     1021 taaaaatgat ttaaaaccac gtgatatcgt tactcgcgaa gcaattgatg atgcatttgc
     1081 acttgatatg gctatgggtg gttcaacaaa cacggtactg catacgttag ccattgccaa
     1141 tgaagctggt attgattatg acttagagcg cattaatgct attgccaaac gcacgccata
     1201 tttatcaaaa atagcaccta gttcatcgta ttcaatgcat gatgtgcatg aagctggtgg
     1261 cgtcccagca attattaatg aattgatgaa gaaagatggc acgttacacc cagatagaat
     1321 cacagttact ggcaaaacgt tacgtgaaaa taacgaaggc aaagaaatta agaactttga
     1381 tgtcattcac cctcttgatg caccatatga tgcacaaggc ggtttatcta tcttatttgg
     1441 taatatcgcc cctaaaggcg cagttattaa agttggcggc gttgatccat ctatcaaaac
     1501 atttactggg aaagcaattt gtttcaattc gcatgatgaa gctgttgaag caatagacaa
     1561 tcgtaccgtt cgtgcaggcc acgtcgttgt cattagatat gaaggaccta aaggtggacc
     1621 aggtatgcct gaaatgttag cacctacttc ctctattgtt ggtcgcggct taggtaaaga
     1681 tgttgcatta attactgatg ggcgtttttc cggtgccaca agaggtattg cagttggtca
     1741 tatttcccct gaagctgcat ctggtggacc aattgcctta attgaagatg gagatgagat
     1801 tactattgat ttaacaaatc gtacattaaa cgtaaaccag cctgaagatg ttctagcgcg
     1861 tcgccgagaa tctttaacac cgtttaaagc gaaagtaaaa acaggttatc tagctcgtta
     1921 tactgcccta gtaactagcg caaatacagg tggcgtcatg caagtccctg agaatttaat
     1981 ttaatttatt tttatattgg agatggttaa aatgtctaaa actcaacatg aagtaaacca
     2041 aaatattgac cctttaaaaa tggctgaatc acttgaactt gaacaactaa atgaaaaaac
     2101 tttaaatgac atgcgttcag gatcagaagt gctagtagaa gctctactta aagaaaatgt
     2161 ggattattta ttcggttatc ctggtggtgc cgtactacct ttatacgaca cattttatga
     2221 tggtaaaatc aaacatattt tagcaagaca cgaacaaggt gctgttcatg ctgcagaagg
     2281 ttatgcacgt gtatctggta aaactggcgt cgttgtagtt acaagcggtc caggtgcaac
     2341 taatgtaatg acaggtatta cggatgcaca ttgcgactct ttacctctag ttgtattcac
     2401 tggacaagtt gctacaccag gcattggtaa agatgcattc caagaagcgg atattctatc
     2461 tatgacttca ccaattacaa aacaaaatta tcaagtgaaa cgtgttgaag atatccctaa
     2521 aatcgtacac gaagctttcc atgtagctaa ttctggacgc aaaggtcctg tagtgattga
     2581 ttttccaaaa gatatgggtg ttttagctac aaatgtggat ttatgcgacg aaatcaatat
     2641 tccaggttat gaagttgtta cagaaccaga aaataaagac attgacactt tcatctcact
     2701 tttaaaagaa gcgaaaaagc ctgtcgtatt agccggcgca ggtattaatc aatcaaaatc
     2761 aaatcaatta ttaacacagt ttgttaataa acatcaaatc ccaacagtta ctactttact
     2821 aggtctaggt gctgtacctt atgaggatac actattttta ggtatgggag gtatgcatgg
     2881 ttcttatgct agtaacatgg cattaactga gtgtgattta ctcattaatt taggtagccg
     2941 cttcgatgat agattagcaa gcaaacctga tgcctttgca cctaacgcca aaattgtaca
     3001 tgtagatatt gatccttcag aaatcaataa agttattcat gtagatttag gtattattgc
     3061 agactgtaaa agatttttag aatgtttaaa tgataaaaat gttgagacta tagaacacag
     3121 tgactgggtt aaacattgtc aaaataataa gcagaaacac ccatttaaac ttggtgaaga
     3181 agatcaagta ttttgtaagc cacaacaaac aatcgaatat atcggcaaaa ttacaaatgg
     3241 tgaagcaatt gttactacag acgtgggaca acatcaaatg tgggcagctc aattttatcc
     3301 atttaaaaat cacggacaat gggttacaag cggtggttta ggaacaatgg gattcggtat
     3361 tccttcgtca attggtgcca aattagctaa tcctgataaa acagtcgtat gtttcgtcgg
     3421 tgacggtggt ttccaaatga caaaccaaga aatggcactt ttacccgaat atggtttaga
     3481 tgtcaaaatc gtactaatca ataatggaac attaggtatg gttaaacaat ggcaagataa
     3541 gttctttaat caacgcttct cacactcagt atttaatggt caacctgatt ttatgaaaat
     3601 ggcagaagca tatggcgtca aaggtttctt aatcgataag ccagaacaac tggaagaaca
     3661 attagatgca gcgtttgctt atcaaggacc agctttaatt gaggttcgta tttcccctac
     3721 tgaagctgta accccaatgg ttccgagtgg caaatcaaat catgaaatgg agggcttata
     3781 atgacaagaa ttcttaaatt acaagttgcg gatcaagtca gcacgctaaa tcgaattaca
     3841 agtgcttttg ttcgcctaca atataatatc gatacattac atgttacaca ttctgaacaa
     3901 cctgggattt ctaacatgga aattcaagtc gatattcaag atgatacatc acttcatata
     3961 ttaattaaaa aattaaaaca acaaattaat gttttaacgg ttgaatgcta cgaccttgtt
     4021 gataacgaag cttaatttta agacaaaggc aatgatgcgc taattagtta tagataaatc
     4081 ataggctgct agttaacatc tgccactatt acaaagttat atttcagaat tttcgaaaaa
     4141 caaaatattt aattatttgg aggaatttat tatgacaaca gtttattatg atcaagatgt
     4201 aaaaacggac gctttacaag gcaaaaaaat tgcagtagta ggttatggat cacaaggtca
     4261 cgcgcatgca caaaacttaa aagacaatgg atatgatgta gtcatcggca ttcgcccagg
     4321 tcgttctttt gacaaagcta aagaagatgg atttgatgtg ttccctgttg cagaagcagt
     4381 taagcaagct gatgtaatta tggtgctatt acctgatgaa attcaaggtg atgtatacaa
     4441 aaacgaaatt gaaccaaatt tagaaaaaca taatgcgctt gcatttgctc atggctttaa
     4501 cattcatttt ggtgttattc aaccaccagc tgatgttgat gtatttttag tagctcctaa
     4561 aggaccgggt catttagtta gacgtacatt tgttgaaggt tctgctgtac catcactatt
     4621 tggtattcaa caagacgctt caggtcaagc acgtaatatt gctttaagtt atgcaaaagg
     4681 tattggtgca actcgtgcag gtgttattga aacaacattt aaagaagaaa ctgagacaga
     4741 tttatttggt gaacaagcag tactttgcgg tggtgtatcg aaattaattc aaagtggctt
     4801 tgaaacatta gtagaagcgg gttatcaacc agaattagct tattttgaag tattacatga
     4861 aatgaaatta atcgttgatt tgatgtatga aggcggtacg gaaaatgtac gttactcaat
     4921 ttcaaatact gctgaatttg gtgactatgt ttcaggacca cgtgttatca caccagatgt
     4981 taaagaaaat atgaaagctg tattaactga tatccaaaat ggtaacttca gtaatcgctt
     5041 tatcgaagac aataaaaatg gattcaaaga attttataaa ttacgcgaag aacaacatgg
     5101 tcatcaaatt gaaaaagttg gtcgtgaatt acgcgaaatg atgcctttta ttaaatctaa
     5161 aagcattgaa aaataagata gacctacaat gaggagttgt taaatatgag tagtcatatt
     5221 caaatttttg atacgacact aagagacggt gaacaaacac caggagtgaa ttttactttt
     5281 gatgaacgct tgcgtattgc attgcaatta gaaaaatggg gtgtagatgt tattgaagct
     5341 ggatttcctg cttcaagtac aggtagcttt aaatctgttc aagcaattgc acaaacatta
     5401 acaacaacgg ctgtatgtgg tttagctaga tgtaaaaaat ctgacatcga tgctgtatat
     5461 gaagcaacaa aagatgcagc gaagccggtc gtgcatgttt ttatagcaac atcacctatt
     5521 catcttgaac ataaacttaa aatgtctcaa gaagacgttt tagcatctat taaagaacat
     5581 gtcacatacg cgaaacaatt atttgacgtt gttcaatttt cacctgaaga tgcaacgcgt
     5641 actgaattac cattcttagt gaaatgtgta caaactgccg ttgacgctgg agctacagtt
     5701 attaatattc ctgatacagt cggctacagt taccatgatg aatatgcaca tattttcaaa
     5761 accttaacag aatctgtaac atcttcaaat gaaattattt atagtgctca ttgccatgac
     5821 gatttaggaa tggctgtttc aaatagttta gctgcaattg aaggcggtgc gagacgaatt
     5881 gaaggcactg taaatggtat tggtgaacga gcaggtaatg cagcacttga agaagtcgcg
     5941 cttgcactat acgttcgaaa tgatcattat ggtgctcaaa ctgctcttaa tctcgaagaa
     6001 actaaaaaaa catcggattt aatttcaaga tatgcaggta ttcgagtgcc tagaaataaa
     6061 gcaattgttg gccaaaatgc atttagtcat gaatcaggta ttcaccaaga tggcgtatta
     6121 aaacatcgtg aaacatatga aattatgaca cctcaacttg ttggtgtaag cacgactgaa
     6181 cttccattag gaaaattatc tggtaaacac gccttctcag agaagttaaa agcattaggt
     6241 tatggcattg ataaagaagc gcaaatagat ttatttaaac aattcaaggc cattgcggac
     6301 aaaaagaaat ctgtttcaga tagagatatt catgcgatta ttcaaggttc tgagcatgag
     6361 catcaagcac tttataaatt ggaaacactg caactacaat atgtctctag cggccttcaa
     6421 agtgctgttg ttgttgttaa agataaagag ggtcatattt accaggattc aagtattggt
     6481 actggctcaa tcgtagcaat ttacaatgca gttgatcgta ttttccagaa agaaacagaa
     6541 ttaattgatt atcgtattaa ttctgtcact gaaggtactg atgcccaagc agaagtacat
     6601 gtaaatttat tgattgaagg taagactgtc aatggctttg gtattgatca tgatatttta
     6661 caagcctctt gtaaagcata cgtagaagca catgctaaat ttgcagctga aaatgttgag
     6721 aaggtaggta attaattatg acttataaca ttgttgccct acctggtgat ggaatcggtc
     6781 cagaaatttt gaacggatct ctatcattgc ttgaaattat aagtaataaa tataacttta
     6841 attatcaaat agagcaccac gaatttggtg gtgcctctat tgatacattc ggcgaacctt
     6901 taactgagaa aaccttaaat gcgtgtaaaa gagcagatgc tattttactg ggtgcaatcg
     6961 gtggacctaa atggacagat cctaacaatc gaccagaaca aggattatta aaattgcgta
     7021 aatccttaaa tttatttgca aatatacgcc ccactaccgt tgtcaaaggc gctagttctt
     7081 tatcaccttt aaaggaagaa cgcgttgaag gcacagattt agttatagtc cgtgaattga
     7141 caagtggtat ttattttgga gaacctagac attttaataa tcacgaggcc ttagattctc
     7201 ttacttatac aagagaagaa atagaacgca ttgttcacgt agcatttaaa ttggccgctt
     7261 caagacgagg aaaactaaca tcagttgata aagaaaatgt attagcttct agtaaattgt
     7321 ggcgcaaagt cgtaaatgaa gtaagtcaat tatatccaga agtaacagta aatcacttac
     7381 ttgttgatgc ttgtagtatg catttaatca caaatccaaa acaatttgac gtcatcgtat
     7441 gtgaaaactt atttggcgat attttaagtg atgaagcttc agtgattcct ggttcacttg
     7501 gtttatcacc ttctgctagt tttagtaacg atggtccaag attgtatgag cctattcatg
     7561 gatcagcacc agatattgca ggtaaaaacg ttgccaatcc atttggaatg attctatctt
     7621 tagcgatgtg tttacgtgaa agcttaaatc aaccagatgc tgcagatgaa ttagaacaac
     7681 atatttataa catgattgaa catgggcaaa cgacagcaga tttaggcggc aaattgaata
     7741 ctactgatat tttcgaaatt ctatctcaaa aattgaatca ctaaggggga gatgtaaatg
     7801 ggtcaaacat tatttgacaa ggtgtggaac agacatgtgt tatacgggaa attgggcgaa
     7861 ccgcaactat tatacattga tttacacctt atacatgaag ttacttctcc tcaagcattt
     7921 gaaggactta ggcttcaaaa cagaaaatta agacgcccag atttaacatt tgcaacactc
     7981 gatcacaatg ttcctactat tgatatattc aatattaaag atgaaattgc aaacaaacaa
     8041 atcacaacat tacaaaaaaa cgccatagat tttggggtgc atatttttga tatgggttct
     8101 gatgaacaag gtattgttca catggtagga cctgagacag gacttacaca gcctggaaag
     8161 acaatcgttt gtggtgactc tcacacagca acacatggag catttggtgc tattgcattt
     8221 ggaattggaa caagtgaagt tgaacatgtt ttcgcaactc aaacgctatg gcaaacaaaa
     8281 cccaaaaact taaaaatcga tattaatggt accttaccaa caggcgtcta tgctaaggac
     8341 attattctgc atttaattaa aacgtatggt gttgactttg gtacaggcta tgctttggaa
     8401 tttactggcg aaacaattaa aaacctttca atggacggtc gaatgactat ttgtaacatg
     8461 gctatcgaag gtggtgccaa atacggcata atccaacctg atgatataac atttgaatat
     8521 gttaaaggga gaccatttgc cgataacttc gttaaatcag ttgataagtg gcgtgagcta
     8581 tattctgatg acgacgcgat atttgatcgt gtaattgaac ttgatgtttc aacattagaa
     8641 ccacaagtga catggggaac taatcctgaa atgggtgtta atttcagtga accattccct
     8701 gaaatcagtg atatcaacga tcaacgtgcg tatgattata tggggttaga accaggacaa
     8761 aaagctgaag atatcgactt agggtatgtt ttcctcggtt catgtacgaa tgctagacta
     8821 tcagatttga ttgaagctag tcatattgtt aaaggaaata aagttcaccc aaatattaca
     8881 gctattgtcg tgccaggttc tcgtacagta aaaaaagaag ctgaaaaatt aggtctagat
     8941 actatcttta aaaatgcagg atttgaatgg cgtgaaccag gatgttcaat gtgtttaggc
     9001 atgaatcctg accaagtacc tgagggcgta cattgtgcat ctacaagtaa tcgaaacttt
     9061 gaaggacgac aaggtaaagg tgcaagaaca catttagtat cccctgctat ggcagcagca
     9121 gcagctattc atggtaaatt tgtggacgta agaaaggtgg ttgtttaaat ggcagcaatc
     9181 aaacctatta caacatataa aggtaaaata gtccctctct tcaacgacaa tatcgataca
     9241 gaccaaatca ttcctaaggt acacttaaag cgtatttcaa aaagtggttt cggtccattt
     9301 gcttttgatg aatggcggta cttacctgat ggttcagata atccagattt caatcctaac
     9361 aaaccacaat ataaaggggc ttctatttta attacaggag ataattttgg atgcggttca
     9421 agtcgtgaac atgctgcttg ggctcttaag gactatggtt ttcatattat tattgcagga
     9481 agtttcagtg acatatttta tatgaattgt acaaaaaatg caatgttacc aattgtttta
     9541 gaaaaaaatg cacgtgaaca tcttgctaaa tatgttgaaa ttgaggtcga tttaccaaat
     9601 caaactgtgt catcaccaga caagcgtttc cattttgaaa ttgatgaaac ttggaaaaat
     9661 aaacttgtaa atggcttaga tgacattgca atcaccctac aatatgaatc attaatagaa
     9721 aaatatgaaa aatcacttta agggagttga atattatgac agtcaaaaca acagtttcta
     9781 cgaaagatat cgatgaagca tttttaagac ttaaagatat tgtcaaagaa acacctttac
     9841 aattagacca ttacttatct caaaagtatg attgtaaagt ttatttaaaa cgagaagatt
     9901 tacaatgggt acgttctttt aaattaagag gtgcttacaa cgctatttct gttttgtcag
     9961 atgaagctaa aagtaaaggt attacatgtg cgagtgcagg taatcatgct caaggtgttg
    10021 cctatacagc taaaaaactt aatttaaacg ctgttatctt tatgccagtc actacacctt
    10081 tacaaaaggt aaatcaagta aagttctttg gaaatagtaa cgttgaagtt gtactcactg
    10141 gtgatacatt tgatcactgt ttagctgaag ctttaactta tacaagtgaa catcaaatga
    10201 actttataga tccattcaat aatgttcata caatttctgg acaaggtacg cttgctaaag
    10261 aaatgctaga acaatcaaag actgacaatg ttaactttga ttatttattt gccgctattg
    10321 gtggtggtgg attaatttca ggtattagta cttactttaa aacctattca cctactacga
    10381 aaattatagg tgttgaacct tcaggtgcaa gtagtatgta tgaatctgtt gtcgtaaata
    10441 atcaggtagt cacattgcct aatatcgata aatttgtgga cggtgcatct gtagctagag
    10501 ttggcgatat tacatttgaa attgcaaaag aaaatgtaga tgattacgtt caagtagatg
    10561 aaggtgcagt ttgttctacg attttagaca tgtattcaaa acaagcaatt gtagcagaac
    10621 ctgctggcgc attaagtgtg agtgctcttg aaaactacaa agaccacatt aagggtaaaa
    10681 ctgttgtttg tgtcattagt ggtggtaata atgatattaa tcgtatgaag gaaattgaag
    10741 aacgttcttt attatatgaa gaaatgaaac attattttat tttaaacttc cctcaacgcc
    10801 ctggtgcatt aagagaattt gtaaatgatg ttttaggccc acaagacgat attacaaaat
    10861 ttgaatactt gaaaaaatct tctcaaaata caggtactgt cattattggt attcaactta
    10921 aagatcatga tgatttaata caactcaaac aacgtgtaaa
//
LOCUS       DAMZLE010000087        10700 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMZLE010000087 DAMZLE010000000
VERSION     DAMZLE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.088"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            164..1693
                     /gene="aur"
                     /locus_tag="POB92_001891"
     CDS             164..1693
                     /gene="aur"
                     /locus_tag="POB92_001891"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDI6579375.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2060..2509
                     /locus_tag="POB92_001892"
     CDS             2060..2509
                     /locus_tag="POB92_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDI6579376.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2512..2679
                     /locus_tag="POB92_001893"
     CDS             2512..2679
                     /locus_tag="POB92_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579377.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGIFE"
     gene            2856..4091
                     /gene="arcA"
                     /locus_tag="POB92_001894"
     CDS             2856..4091
                     /gene="arcA"
                     /locus_tag="POB92_001894"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDI6579378.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4124..5134
                     /gene="argF"
                     /locus_tag="POB92_001895"
     CDS             4124..5134
                     /gene="argF"
                     /locus_tag="POB92_001895"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6579379.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5225..6655
                     /gene="arcD"
                     /locus_tag="POB92_001896"
     CDS             5225..6655
                     /gene="arcD"
                     /locus_tag="POB92_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDI6579380.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6672..7613
                     /gene="arcC"
                     /locus_tag="POB92_001897"
     CDS             6672..7613
                     /gene="arcC"
                     /locus_tag="POB92_001897"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6579381.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7712..8416
                     /locus_tag="POB92_001898"
     CDS             7712..8416
                     /locus_tag="POB92_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI6579382.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLFCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8766..>10700
                     /gene="clfB"
                     /locus_tag="POB92_001899"
     CDS             8766..>10700
                     /gene="clfB"
                     /locus_tag="POB92_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDI6579383.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTAKPMST
                     QTSNTTTTEPASTNETPQLTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTAKDFQLEKTTFDPNQSGNTFMAANFTVTGQVKSGDYFTAKLPDSVTGNGDVDYSNS
                     NNTMPIADIVNDKNEVVAKATYDILTKTYTFVFTDYVNDKQNINGKFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKDKITYKYQNVASINFGDINKTYVVLVEGHYDKTGKNLKTQVIQEN
                     VDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPEPTPDPEPSPDPDPDSDSDSDSGSDSDSDSDSDSDSDSDSGSDSDSDSDSDSES
                     DSESDSDSDSESDSDSDSDSE"
BASE COUNT         3971 a         1542 c         2050 g         3137 t
ORIGIN      
        1 tttatttagt tacagataac atttttaaaa aagaaaagaa tcaataaata aaatcaacga
       61 acaaaaagta tcgaaataaa tagaaataat catttacttt tctgaaaaat taaattaata
      121 ttttatttat aagctgtttt taagatttca ggaggaatga aatgtgagga aattttcaag
      181 atatgcattt acaagtatgg cagcagtaac attgttgagc actttatcac cagcagcatt
      241 agcgattgat tcaaaaaata aaccagctaa ttctgatatt aaatttgagg tgactcaaaa
      301 gagtgatgcg gtcaaagcat taaaagaatt gcctaaatcc gaaaatgtaa aaaatattta
      361 tcaagattac gctgttactg atgtaaaaac tgataaaaaa ggatttacgc attatacatt
      421 gcaaccgagt gttgatggtg ttcatgcacc tgacaaagaa gtgaaagtac acgcagacaa
      481 atcaggaaaa gtcgttttaa tcaatgggga tactgatgcg aagaaagtaa agccaacgaa
      541 taaagtgaca ttaagtaaag atgacgcagc cgacaaagca tttaaagcag ttaagattga
      601 taagaataaa gcgaaaaatc ttaaagataa agtcattaaa gaaaacaaag ttgaaatcga
      661 tggtgacagt aataaatacg tttataatgt tgagttaatt acagtgacac cagaaatttc
      721 acattggaaa gttaaaattg atgctcaaac tggcgaaatt ttagaaaaaa tgaacttagt
      781 taaagaagct gcagaaactg gtaaaggaaa aggtgtactt ggcgatacaa aagatatcaa
      841 tatcaatagt attgacggtg gatttagcct agaagattta acgcatcaag gtaaattatc
      901 agcatttagc tttaatgatc aaacaggtca agcaacattg attactaatg aagatgaaaa
      961 cttcgtaaaa gatgagcaac gtgctggcgt agatgcaaat tattacgcta aacaaacata
     1021 tgattattac aaagacacat ttggtcgtga atcatatgac aaccaaggta gtccaattgt
     1081 ttcattaacg catgttaata actacggtgg tcaagataac agaaataatg ccgcatggat
     1141 tggtgacaaa atgatctatg gtgatggtga tggtcgcaca ttcacaagtt tatcgggtgc
     1201 aaatgacgta gtagcacacg aattaacaca cggtgtgaca caagagactg caaacttaga
     1261 atataaggac cagtcaggcg ctctaaatga aagcttttca gatgtttttg gatactttgt
     1321 agatgacgag gatttcttaa tgggtgaaga tgtctacaca cctggaaaag agggagacgc
     1381 tttacgcagc atgtcaaacc cagaacaatt tggtcaacca gctcatatga aagactatgt
     1441 attcactgaa aaagataatg gtggcgtaca tacgaattct ggaattccaa ataaagcagc
     1501 ttataacgtg attcaagcaa tagggaaatc taaatcagaa caaatttact accgagcatt
     1561 aacggaatac ttaacaagta attcaaactt caaagattgt aaagatgcat tataccaagc
     1621 ggccaaagat ttatatgacg agcaaacagc tgaacaggtg tatgaagcat ggaatgaagt
     1681 aggcgtggag taaaaatata taaacaagaa gaagtaatgt taaacactta taaataatta
     1741 aattttaaat acagcttata tatggggtat acctacattg ataattgact ttgtaggtat
     1801 accctctctt tgtaattaga tgagaggagt gtgagggttg tctgccgaaa gactactcgg
     1861 cagtctaaaa tcattacaag tagtagatat gtgataatta aatgctgact tagaatacaa
     1921 aattcatttt aaaagttgtc acaaaaaatt tacatgtatt tttattatct tttgcaaaac
     1981 aaagtgttaa attataaatg aaacatgcat gaatttattt tttaatacaa gaaacgtaac
     2041 taccaaagga gtttacaata tgaagaaaag taaacgatta gaaattgttt ctacaatagt
     2101 taaaaagcat aagatttata aaaaagaaca aatcatttca tatattgaag aatattttgg
     2161 tgtaagatat agtgcaacaa caattgctaa agacttgaaa gaacttaata tatatcgtgt
     2221 ccctatcgat tgtgaaacat ggatttataa agctattaat aatcaaacag aacaagagat
     2281 gagagaaaag tttagacact attgtgaaca tgaagttcta agttcaatca tcaatggttc
     2341 atacattatc gttaaaacct cacctggttt cgcccaaggc ataaactatt ttatcgatca
     2401 gctaaatata gaagagatat taggtacggt gagtggaaat gacactacat taatcttaac
     2461 tgcctcaaat gatatggcag aatacgtata tgcaaaatta tttaaataga catgtatcaa
     2521 atgaataata aaaatttgtt tcgtatcacg tgtactcaag ttagttacca aatattaact
     2581 tgtgtacgcg tttttttatg gaaagaaaga attcatagtc attcaattga ctgtataaaa
     2641 aactttatac aacatgtttt tatgggtatt tttgaataaa aaatgtatat tttgacccaa
     2701 aataccttta tttatgtata aaaatccatt attatgtatt gtataacaaa aagatatgaa
     2761 attttcgact ttctttatgt gaatataatc acatgtaagc gtttgaagat tgtctatact
     2821 ctaaatgaat tcaaagataa aaggaggaaa tagacatgac agatggtcca attaaagtaa
     2881 atagcgaaat tggagcttta aaaactgtgt tacttaagcg tcctggaaaa gaattagaaa
     2941 atttagtacc tgattattta gatggattac tatttgatga tattccatat ttagaagtag
     3001 ctcaaaaaga gcatgaccat tttgcgcagg tgctaagaga agagggtgtt gaagtacttt
     3061 accttgagaa gttagcagct gaaagtattg aaaatcctca agtaagaagt gaatttattg
     3121 atgatgtatt agcagagtct aaaaaaacaa tattaggtca tgaagaagaa attaaggcat
     3181 tatttgcgac actttctaat caagaacttg tagataaaat aatgtcaggg gtacgtaagg
     3241 aagaaattaa tccgaaatgt acacatctag tagagtatat ggatgataag tatccattct
     3301 atttagatcc aatgccaaac ctttatttta ctagagatcc acaagcctca ataggacacg
     3361 gtataacaat caatcggatg ttctggagag cacgacgacg agaatcaata tttattcaat
     3421 atattgtaaa gcatcatcct agatttaaag atgcgaatat tccaatctgg ttagatcgag
     3481 attgcccatt caatattgaa ggcggcgatg aacttgtttt atctaaagat gtcttggcta
     3541 taggcgtttc agaacgtaca tctgcacaag ctattgaaaa gttagcgcga cgtatttttg
     3601 aaaatccgca ggcgacgttt aaaaaagtag tagcaattga aattccaact agtcgaactt
     3661 ttatgcactt agatacagta tttacaatga tagattatga caaatttaca atgcattcag
     3721 ccattttaaa ggcagaaggc aatatgaata tatttattat tgaatatgat gacgtaaata
     3781 aagatattgc catcaaacaa tctagtcatt taaaagatac tttagaagac gtactaggta
     3841 tagatgatat ccaattcatt ccaacaggaa atggtgatgt cattgatggt gctagagagc
     3901 aatggaatga tggctcaaat acattatgta taagaccagg cgttgtagtg acttacgata
     3961 gaaactatgt atcgaatgat ttattgagac aaaaaggcat taaagtcatt gaaatatctg
     4021 gtagcgagtt ggtacgtgga cgtgggggcc ctagatgtat gagtcaacca ttattcagag
     4081 aagacattta acgtaataaa ttatagaaac gagggtgaaa ataatgacag aaattcaaaa
     4141 accgtatgat ttaaaaggca gatcattatt aaaagaaagt gattttacca aagcagaatt
     4201 cgaaggactt attgattttg caattacatt aaaagagtat aagaaaaacg gtattaagca
     4261 tcactactta tctggaaaaa atattgcact actattcgaa aagaattcga cgagaacgcg
     4321 tgctgcgttt acagttgcgt ctattgattt aggtgcgcat ccagaatttt tagggaaaaa
     4381 tgatattcaa ttaggcaaaa aagaatctgt agaggatact gcgaaagtat taggtagaat
     4441 gttcgatggt attgaattcc gtggtttttc acaacaagct gttgaagatt tagcgaagtt
     4501 ctctggtgtg ccggtgtgga atggattaac agacgattgg catcctacac aaatgttagc
     4561 tgattttatg acaataaaag agaattttgg atatctagaa ggaataaact taacttacgt
     4621 tggagatgga cgtaataata ttgcgcattc attaatggta gcaggtgcta tgttaggtgt
     4681 taatgtaaga atttgtacac ctaaatcatt aaatccaaaa gaggcatatg ttgatattgc
     4741 aaaagaaaaa gcgagtcaat atggtggttc agtcatgatt acggataata ttgcagaagc
     4801 agttgaaaat acagatgcta tatatacaga tgtttgggta tcgatgggtg aagaaagtga
     4861 atttgaacaa cgtattaatt tattaaaaga ctatcaagtg aatcaacaga tgtttgattt
     4921 aacaggtaaa gattcaacga tattcttaca ttgtttacca gcattccatg atacaaatac
     4981 actttatgga caagaaattt atgaaaaata tggattagct gaaatggaag ttacagacca
     5041 aatctttaga agtgaacatt caaaagtgtt tgatcaagct gaaaatagaa tgcatacaat
     5101 taaggcagta atggcagcaa cattggggag ttaatcacta aatggaacga tatgaatatg
     5161 atgtgtctga tgatataagt gtcatgtaca gacacctcat attggtatta aaggagaaat
     5221 gaatatgaac gaatcaggag ataacaaact cagtaaatct tctttaattg gactagttat
     5281 aggatccatg attggtggcg gtgcgttcaa tataatgtct gatatgggcg gtaaagccgg
     5341 tggattagcc attattattg gttggattat tacagctata ggaatgattt cattagcgtt
     5401 cgtatttcaa aatttaacca atgaacggcc ggagctagac ggtggtattt atagttatgc
     5461 tcaagcagga tttggcgatt ttgtaggatt tatcagtgct tggggatatt ggttctcagc
     5521 gtttttaggc aatgttgcct atgcaacact attgatgtca gcagtaggta actttttccc
     5581 gatttttaaa ggaggcaaca cattaccaag tattattgtc gcctcgttac tactctgggg
     5641 tgtccatttc ttgattttaa aaggcgttga aacagcagca tttatcaata gtattgttac
     5701 tgttgcaaag ttaataccga ttttacttgt aatcatatgc atgataattg cattcaattt
     5761 tgacactttt aaaacaggct ttttcggtat gacgtcagag ggtgtattgc catttagttg
     5821 ggcgagcaca atgagccaag ttaaaagtac gatgctagtg acagtttggg tgtttatcgg
     5881 tatcgagggt gcagtaattt tttctagtag agctaaaaat aaaaaagatg taggtagtgc
     5941 cacggttata ggacttatat cagttttaat tatctatttc ttattaactg tattagctca
     6001 aggcgtgatt ttgcaaaatc atatttctca attagattcg ccaagtatgg cacaggtgct
     6061 tgcaactatt gtaggtggtt ggggatctac acttgtaaat attggtttaa ttatttcggt
     6121 actaggtgca tggttaggat ggacattgct tgctggtgaa ttacctttca ttgttgcaaa
     6181 agatggatta tttccaaagt ggtttgctaa agaaaataaa aatggagcac ctgtaaatgc
     6241 actgcttatt accaatatat tagtacaatt atttttaata agtatgctat ttacacagag
     6301 tgcgtatcaa tttgcatttt cactagcatc aagtgctatt ttataccctt acatgttcag
     6361 tgcattttac caagttaaat acactttaga gcatcgacag caagcaacta ctaagcaatg
     6421 gacgattggc atcatagcct caatttatgc tatatggctt atatatgcag caggtatcaa
     6481 ttacttatta ttgacgatgt tactttatat tccagctctt cttgtttatt caatcgttca
     6541 aaagaataat cagacacgtt tgattaaatc agactatatt ctttttatga ttattatcgt
     6601 acttgcagtt atcggattaa ttaagttatt gatgggaacg ataaatgttt tttaaaagga
     6661 gcgacaaaaa tatgaaagag aaaattgtca ttgcattagg cggtaatgcg atacagacaa
     6721 aagaagcaac agctgaagca caacaaacag ctattagacg tgcgatgcaa aaccttaaac
     6781 ctttatttga ttcaccagcg cgtattgtca tttcacatgg taatggccca caaattggaa
     6841 gtttattaat ccaacaagct aaatcgaaca gtgacacaac gccggcaatg ccattggata
     6901 cttgtggtgc aatgtcacag ggtatgatag gctattggtt ggaaactgaa atcaatcgca
     6961 ttttaactga aatgaatagt gatagaactg taggcacaat cgttacacgt gtggaagtag
     7021 ataaagatga tccacgattc aataacccaa ccaaaccaat tggtcctttt tatacgaaag
     7081 aagaagttga agaattacaa aaagaacagc cagactcagt ctttaaagaa gatgcaggac
     7141 gtggttatag aaaagtagtt gcgtcaccac tacctcaatc tatactagaa caccagttaa
     7201 ttcgaacttt agcagacggt aaaaatattg tcattgcatg cggtggtggc ggtattccag
     7261 ttataaaaaa agaaaatacc tatgaaggtg ttgaagcggt tatagataaa gattttgcta
     7321 gtgagaaatt agcaacgctg attgaagcag ataccttaat gattcttacg aatgtagaaa
     7381 atgtatttat taactttaat gaacctaatc aacaacaaat cgatgatatt gatgtagcaa
     7441 cactgaaaaa atacgcggca caaggtaagt ttgcggaagg atcgatgttg ccaaaaatag
     7501 aagctgcgat acgatttgtt gaaagtgggg aaaacaaaaa agttatcatt accaatttag
     7561 agcaggcata cgaagctttg attggtaata aaggtacaca cattcacatg tagcacttta
     7621 tcacgcgaca aaacattaaa tatgtttctc cgttgattca aatgaaaaag ttgtctgctg
     7681 acactttgca aggtttgaag gagtttaact tatgacagaa aactttattt tgggtagaaa
     7741 taataaatta gaacatgaac taaaggcatt agcagattac attaatatcc catatagtat
     7801 attacaacca tatcaaagtg aatgttttgt cagacattat acgaaaggcc aagttattta
     7861 tttttcgcca caagaaagta gcaatattta ctttttaatt gaaggtaaca ttattagaga
     7921 acattacaat caaaatggag atgtatatcg ttattttaat aaagagcaag tattatttcc
     7981 aatcagtaac ttatttcatc cgaaagaggt taacgaattg tgtacagcat taaccgattg
     8041 tacagttctt ggattgccta gagaattgat ggcctttttg tgcaaagcta atgatgatat
     8101 atttttgaca ctttttgcat taataaatga taatgagcag caacacatga actataacat
     8161 ggcattaaca agtaaatttg ctaaagatcg aattattaaa ttgttttgtc atctatgtca
     8221 gacagtaggg tacgatcaag atgaatttta tgaaatcaaa cagtttttaa ctattcaact
     8281 catgagtgat atggctggga tttcccggga aacagctggt catattattc atgaacttaa
     8341 agatgaaaaa cttgttgtta aagatcataa aaattggtta gtaagcaaac atttattcaa
     8401 tgatgtatgt gtttaatata caatgtaaaa tgaataagtt gaacatgagg tctaacgtac
     8461 atttatacgt taggcctttt ttgctagcat gatgaataat ttaaaatgtt agttaaattt
     8521 gattgttgaa attacagtaa aatttaaggt gatgaaaaat ttagaacttc taagtttttg
     8581 aaaagtaaaa catttgcaat agtgtaaaaa tggtatattg atttttgcta gttaacagaa
     8641 aattttaagt tatataaata ggaagaaaac aaattttacg taattttttt cgaaaagcaa
     8701 ttgatataat tcttatttca ttatacaatt tagactaatc tagaaattga aatggagtaa
     8761 tatttttgaa aaaaagaatt gattatttgt cgaataagca gaataagtat tcgattagac
     8821 gttttacagt aggtaccaca tcagtaatag taggggcaac tatactattt gggataggca
     8881 atcatcaagc acaagcttca gaacaatcga acgatacaac gcaatcttcg aaaaataatg
     8941 caagtgcaga ttccgaaaaa aacaatatga tagaaacacc tcaattaaat acaacggcta
     9001 atgatacatc tgatattagt gcaaacacaa acagtgcgaa tgtagatagc acagcaaaac
     9061 caatgtctac acaaacgagc aataccacta caacagagcc agcttcaaca aatgaaacac
     9121 ctcaactgac ggcaattaaa gatcaagcaa ctgctgcaaa aatgcaagat caaactgttc
     9181 ctcaagaagc aaattctcaa gtagataata aaacaacgaa tgatgctaat agcatagcga
     9241 caaacagtga gcttaaaaat cctcaaacat tagatttacc acaatcatca ccacaaacaa
     9301 tttccaatgc gcaaggaact agtaaaccaa gtgttagaac gagagctgta cgtagtcttg
     9361 cagttgctga acctgtagta aatgctgctg atgctaaagg tacaaatgta aatgataaag
     9421 ttacggcaaa agattttcaa ttagaaaaga ctacatttga ccctaaccaa agtggtaata
     9481 cttttatggc ggcaaacttt acagtgactg gacaagtgaa atcaggggat tattttacag
     9541 cgaagttacc agatagtgta actggtaatg gagacgtgga ttactctaat tcgaataata
     9601 cgatgccaat tgcagatata gtaaacgata aaaatgaagt tgtagcaaaa gcgacatatg
     9661 atattttgac taagacatat acatttgtct ttacagatta tgtaaatgat aagcaaaata
     9721 ttaatgggaa attttcatta ccactattta cagacagagc aaaggcacct aaatcaggaa
     9781 catatgatgc aaatattaat attgcggatg aaatgtttaa taataaaatt acttataact
     9841 atagttcgcc aattgcagga attgataagc caaatggcgc gaacatttct tctcaaatta
     9901 ttggtgtaga tacagcttca ggtcaaaata catacaagca aacggtattt gttaacccta
     9961 agcaacgagt tttaggtaat acgtgggtgt atattaaagg ttaccaagat aaaatcgaag
    10021 aaagtagcgg taaagtaagt gctacagata caaaactgag aatttttgaa gtgaatgata
    10081 catctaaatt atcagatagc tactatgcag acccaaatga ctctaatctt aaagaagtaa
    10141 ctgatcaatt taaggataaa atcacttata aataccaaaa tgtagcaagt attaattttg
    10201 gtgatataaa taaaacgtat gtcgttttag tcgaaggtca ttatgataaa acaggtaaaa
    10261 acttgaaaac gcaagtcatt caagaaaatg ttgacccagc gacaggtaaa gactacagta
    10321 ttttcggttg gaataatgag aatgttgtac gttatggtgg tggaagtgct gatggtgatt
    10381 cagcagtaaa tccgaaagac ccaactccag ggccaccggt tgacccagaa ccaagtccag
    10441 acccagaacc ggaaccaagt ccagacccgg atccggaacc aacgccagat ccagaaccaa
    10501 gcccagaccc ggatccggat tcggattcag acagcgactc aggttcagat agcgattcgg
    10561 attcagacag cgattcagac tcagacagcg actcaggttc agatagcgat tcggattcag
    10621 acagcgactc agaatcagac agtgagtcag attcagatag cgattcagaa tcagatagcg
    10681 actcagattc agatagtgag
//
LOCUS       DAMZLE010000088        10647 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMZLE010000088 DAMZLE010000000
VERSION     DAMZLE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.089"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            208..1692
                     /locus_tag="POB92_001900"
     CDS             208..1692
                     /locus_tag="POB92_001900"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDI6579384.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPCEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYSVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            1673..1933
                     /locus_tag="POB92_001901"
     CDS             1673..1933
                     /locus_tag="POB92_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDI6579385.1"
                     /translation="MALLVNKRFIDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1933..3495
                     /locus_tag="POB92_001902"
     CDS             1933..3495
                     /locus_tag="POB92_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI6579386.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3797..4600
                     /locus_tag="POB92_001903"
     CDS             3797..4600
                     /locus_tag="POB92_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6579387.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDNHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4819..7143
                     /locus_tag="POB92_001904"
     CDS             4819..7143
                     /locus_tag="POB92_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI6579388.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDFESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNYE
                     NDLSHHMPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRITEKRNNAINDHDKLNGQKPNAKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7160..8518
                     /locus_tag="POB92_001905"
     CDS             7160..8518
                     /locus_tag="POB92_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI6579389.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8657..10168
                     /locus_tag="POB92_001906"
     CDS             8657..10168
                     /locus_tag="POB92_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6579390.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDDDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLSKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYHISCSDNFIVKNR"
     gene            complement(10348..10436)
                     /locus_tag="POB92_001907"
     tRNA            complement(10348..10436)
                     /locus_tag="POB92_001907"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10399..10401),aa:Ser,seq:gct)
     gene            complement(10439..10513)
                     /locus_tag="POB92_001908"
     tRNA            complement(10439..10513)
                     /locus_tag="POB92_001908"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10479..10481),aa:Asn,seq:gtt)
BASE COUNT         3825 a         1435 c         2057 g         3330 t
ORIGIN      
        1 ttatgatatt ataccatgtt acaagatggt tttaataatt taagatgagc cataattgtg
       61 aaactaattc ataataacgt atgttttatt tttaatagta gaaattagaa aatgctgatt
      121 agtaggatat aacagtgaaa ttataaattt attaacatca acaaaacgtg tataataaac
      181 atattgtaga aaaaggagcg gttcagtttg gatgcaagta cgttgtttaa gaaagtaaaa
      241 gtaaagcgtg tattgggttc tttagaacaa caaatagatg atatcactac tgattcacgt
      301 acagcgagag aaggtagcat ttttgtcgct tcagttggat atactgtaga cagtcataag
      361 ttctgtcaaa gtgtagctga tcaagggtgt aagttggtag tggtcaataa agaacaatca
      421 ttaccagcta acgtaacaca agtggttgtg ccggacacat taagagtagc tagtattcta
      481 gcacacacat tatatgatta tccgagtcat cagttagtga catttggtgt aacgggtaca
      541 aatggtaaaa cttctattgc gacgatgatt catttaattc aaagaaagtt acaaaaaaat
      601 agtgcatatt taggaactaa tggtttccaa attaatgaaa caaagacaaa aggtgcaaat
      661 acgacaccag aaacagtttc tttaactaag aaaattaaag aagcagttga tgcaggcgct
      721 gaatccatga cattagaagt atcaagccat ggcttagtat taggacgact gcgaggcgtt
      781 gaatttgacg ttgcaatatt ttcaaattta acacaagacc atttagattt tcatggcaca
      841 atggaagcat acggacacgc gaagtcttta ttgtttagtc aattaggtga agatttgtcg
      901 aaagaaaagt atgtcgtgtt aaacaatgac gattcatttt ctgagtattt aagaacagtg
      961 acgccttgtg aagtatttag ttatggaatt gatgaggaag cccaatttat ggctaaaaat
     1021 attcaagaat ctttacaagg tgtcagcttt gattttgtaa cgccttttgg aacttactca
     1081 gtaaaatcgc cttatgttgg taagtttaat atttctaata ttatggcggc aatgattgcg
     1141 gtgtggagta aaggtacatc tttagaaacg attattaaag ctgttgaaaa tttagaacct
     1201 gttgaagggc gattagaagt tttagatcct tcgttaccta ttgatttaat tatcgattat
     1261 gcacatacag ctgatggtat gaacaaatta atcgatgcag tacagccttt tgtaaagcaa
     1321 aagttgatat ttttagttgg tatggcaggc gaacgtgatt taactaaaac gcctgaaatg
     1381 gggcgagttg cctgtcgtgc agattatgtc attttcacac cggataatcc ggcaaatgat
     1441 gacccgaaaa tgttaacggc agaattagcc aaaggtgcaa cacatcaaaa ctatattgaa
     1501 tttgatgatc gtgcagaagg gataaaacat gcaattgaca tagctgagcc tggggatact
     1561 gtcgttttag catcaaaagg aagagaacca tatcaaatca tgccagggca tattaaggtg
     1621 ccacatcgag atgatttaat tggccttgaa gcagcttaca aaaagttcgg tggtggccct
     1681 gttggtcaat aaaagattta ttgatgaagg taaaactatt gatgtttatt tattcgaagc
     1741 attaaatgac cagataatca ttgctatacc agattggttt tggtcatatc agatggcaat
     1801 gacattagat gaagaaactt gttttgaagc aatactcatg caattgtttg tttttaaaga
     1861 agaggaagag gcagaatcga ttgcatcaca actaacagat tggatagaaa catataaaaa
     1921 ggagaaagac taatgaactt aaagcaagaa gttgagtcta gaaagacttt tgcgattatt
     1981 tcacatcccg atgcagggaa aacaacgtta actgaaaaac tattgtactt cagtggtgcc
     2041 attcgtgaag cgggtacagt taaagggaag aagactggta aatttgcgac aagtgactgg
     2101 atgaaagttg aacaagaacg tggtatttct gtaacaagtt cagtaatgca atttgattac
     2161 gatgattata aaatcaatat cttagataca ccaggacatg aagacttttc agaagatacg
     2221 tatagaacat taatggcagt tgacagtgct gtcatggtca tagactgtgc aaaaggtatt
     2281 gaaccacaaa cattgaagtt atttaaagtt tgtaaaatgc gtggtattcc aatctttaca
     2341 ttcattaata aattagaccg agtaggtaaa gaaccatttg aattattaga tgaaatcgaa
     2401 gagacattaa atattgaaac ataccctatg aattggccaa ttggtatggg acaaagtttc
     2461 tttggcatca ttgatagaaa gtctaaaaca attgaaccat ttagagatga agaaaatata
     2521 ttacatttga atgatgattt tgagttggaa gaagatcatg caattacaaa tgatagtgct
     2581 tttgaacaag cgattgaaga attaatgttg gttgaagaag cgggtgaagc ctttgataat
     2641 gacgcgctgt tgagtggaga cttaacacct gtatttttcg gttcagcttt agctaacttt
     2701 ggtgtacaaa atttcttaaa tgcatatgtt gattttgcgc caatgccaaa tgcaagacaa
     2761 acaaaagaag aggttgaagt aagcccgttt gatgattcat tttcaggatt tatctttaaa
     2821 attcaagcca acatggaccc taaacaccgt gatagaattg catttatgcg tgtcgttagt
     2881 ggtgcatttg aacgtggtat ggatgttact ttgcaacgta ctaataaaaa gcaaaagatc
     2941 acacgttcaa cgtcatttat ggcagacgat aaagaaactg tgaatcatgc tgtagcaggc
     3001 gatatcattg gactatatga tactggtaat tatcaaattg gagatacttt agttggtgga
     3061 aaacaaacct acagtttcca agatttacca caatttacgc cagaaatttt tatgaaagtt
     3121 tctgctaaaa acgtcatgaa acagaagcat ttccataaag gtattgaaca attagtacaa
     3181 gaaggtgcga ttcaatacta taaaacatta cacacaaacc aaattatttt aggtgctgtt
     3241 ggtcagttac aatttgaagt tttcgaacat agaatgaaaa atgaatataa tgttgatgtt
     3301 gttatggagc cagtaggccg taaaattgca cgttggattg aaaatgaaga ccaaattaca
     3361 gataagatga acacatcaag atcgatttta gtgaaagata gatatgacga tttagtattc
     3421 ttatttgaaa atgaatttgc aacaagatgg tttgaagaga aattccctga aattaaattg
     3481 tatagtttac tttaacagct caattgtata atcgaatttg ttacattaaa aataattgtt
     3541 tcgttgaaga aaaataaatt gtatatttta aaagaaaaag gtatactatg atgtatcaaa
     3601 tgaataacct atggcatttt gtcagagggg agtaacttaa gaatcatgac cgtataaatg
     3661 attcgacact ttatcgtcat tacgaagata tcttccggta aagtgggcaa tttaaattgc
     3721 ttagtgagac ctttgctatt tatttagcat aggtcttttt gtttgtactt aacttattta
     3781 tttaaaggag ttgtacatgt taatggatcc aagtttgatc ttaccttatt tatgggtact
     3841 tgtcgtttta gtatttttag aaggcttatt agcagcagat aacgcgattg ttatggctgt
     3901 aatggttaag cacttaccac ccgaacaacg taaaaaagct ttgttttacg gtttgttagg
     3961 tgcatttgta tttagatttt tagcattatt cttaattagt attatcgcga acttttggtt
     4021 tattcaagct gcaggagcgg tttacttaat ttatatgtca atcaaaaatc tgtggcagtt
     4081 ctttaaacac ccagaaattg aaagtcctga agctggagat aatcatcatt atgatgaatc
     4141 tggtgaagag attaaagcaa gtaacaaatc attctgggga actgtgttga aaatagaatt
     4201 tgcagatatc gcatttgcca ttgattctat gcttgctgct ttagctattg ctgtaacact
     4261 tcctaaagtt ggtattcact ttggtggtat ggacttaggt cagttcgtag tcatgttcct
     4321 aggtggaatg attggtgtta ttctaatgcg ttatgcagca acatggtttg tagagctatt
     4381 aaacaaatat ccaggacttg aaggtgcagc cttcgcgatc gttggttggg taggtgttaa
     4441 attagttgtc atggtattag cgcacccaga catcgctgta ttgcctgagc acttcccaca
     4501 tggcgtatta tggcaatcta ttttctggac agtactaatt ggattagtaa ttatcggttg
     4561 gttaggttca gttgttaaaa ataaaaaatc gcataaataa ttgatgtgaa gcggacaatc
     4621 ttaatttagt ttaaggttgt cctttttcat ttaattgagt gatttatgaa aaatggattt
     4681 tgaagaatgt gaatcaaaag atgcgatata gtattaagaa aatgtgcctt ttatatttag
     4741 cattttttca atagaaatta tatagatttt aaagcaaatt aggtgttaat gtgtcataat
     4801 gataagtgat tttattgaat ggagtggaca ttagtggata ttggtaaaaa acatgtaatt
     4861 cctaaaagtc agtaccgacg taagcgtcgt gaattcttcc acaacgaaga cagagaagaa
     4921 aatttaaatc aacatcaaga taaacaaaat atagataata caacatcaaa aaaagcagat
     4981 aagcaaatac ataaagattc aattgataag cacgaacgtt ttaaaaatag tttatcatcg
     5041 catttagaac agagaaaccg tgatgttaat gagaataaag ctgaagaaag taaaagtaat
     5101 caggatagta agtcagcata taacagagat cattatttaa cagacgatgt atctaaaaaa
     5161 caaaattcat tagattcagt ggaccaagat acagagaaat caaaatatta tgagcaaaat
     5221 tctgaagcga ctttatcaac taaatcaacc gataaagtag aatcaactga catgagaaag
     5281 ctaagttcag ataaaaacaa agttggtcat gaagagcaac atgtactttc taaaccttca
     5341 gaacatgata aagagactag aattgatttt gagtcttcaa gaactgattc agacagctcg
     5401 atgcagacag agaaaataaa aaaagacagt tcagatggaa ataaaagtag taatctgaaa
     5461 tctgaagtaa tatcagacaa atcaaataca gtaccaaaat tgtcggaatc tgatgatgaa
     5521 gtaaataatc agaagccatt aactttgccg gaagaacaga aattgaaaag acagcaaagt
     5581 caaaatgagc aaacaaaaac ctatacatat ggtgatagcg aacaaaatga caagtctaat
     5641 tatgaaaatg atttaagtca tcatatgcca tcgataagtg atgataaaga taacgtcatg
     5701 agagaaaatc atattgttga cgataatcct gataatgata tcaatacacc atcattatca
     5761 aaaacagatg acgatcgaaa acttgatgaa aaaattcatg ttgaagataa acataaacaa
     5821 aatgcagact cgtctgaaac ggtgggatat caaagtcagt caagtgcatc tcatcgtatc
     5881 actgaaaaaa gaaataatgc tattaatgac catgataaat taaacggtca aaaaccaaat
     5941 gcaaagacat cggcaaataa taatcaaaaa aaggctacat caaaattgaa caaagggcgc
     6001 gctacaaata ataattatag tgacattttg aaaaagtttt ggatgatgta ttggcctaaa
     6061 ttagttattc taatgggtat tattattcta attgttattt tgaatgctat ttttaataat
     6121 gtgaacaaaa atgatcgcat gaatgataat aatgatgcag atgctcaaaa atatacgaca
     6181 acgatgaaaa atgccaataa cacagttaaa tcggtcgtta cagttgaaaa tgaaacatca
     6241 aaagattcat cattacctaa agataaagca tctcaagacg aagtaggatc aggtgttgta
     6301 tataaaaaat ctggagatac gttatatatt gttacgaatg cacacgttgt cggtgataaa
     6361 gaaaatcaaa aaataacttt ttcgaataat aaaagtgttg ttgggaaagt gcttggtaaa
     6421 gataaatggt cagatttagc tgttgttaaa gcgacttctt cagacagttc agtgaaagag
     6481 atagctattg gagattcaaa taatttagtg ttaggagagc caatattagt cgtaggtaat
     6541 ccacttggtg tagactttaa aggcactgtg acagaaggta ttatttcagg tctgaacaga
     6601 aatgttccta ttgatttcga taaagataat aaatatgata tgttgatgaa agctttccaa
     6661 attgatgcat cagtaaatcc aggtaactcg ggtggtgctg tagtcaatag agaaggaaaa
     6721 ttaattggtg tagttgcagc taaaattagt atgccaaacg ttgaaaatat gtcatttgca
     6781 atacctgtta atgaagtaca aaagattgta aaagatttag aaacaaaagg taaaattgac
     6841 tatcccgatg taggtgttaa aatgaagaat attgccagtc taaatagttt tgaaagacaa
     6901 gcagttaaat tgccaggaaa agttaagaac ggtgttgttg tagatcaagt tgacaacaat
     6961 ggtttagcag atcaatctgg tctgaaaaaa ggtgatgtaa ttactgaatt agatggcaaa
     7021 cttttagaag atgatttacg ctttaggcag attatattta gtcataaaga tgacttgaaa
     7081 tcaattacag cgaagattta tagagatggt aaagagaaag aaattaatat taaactaaaa
     7141 taatgttgag gtgaacaacg tgtcaatttt tagccagttt ttaaaaagat caagccctca
     7201 acaaggtatt gtattgtact atatcgtcgc aattgtcatt gcatttttat tattaaactt
     7261 accgtatgtt cataaaccag gtgtagaagt aaatccaatt gacacattat ttgttgccgt
     7321 atccggaatt agtgttactg gattgtctcc gataagtatt gtcgatacct attctacatt
     7381 tggacaatta attatcctcg tgatattaaa tattggtgga attggcgtca tggcaattgg
     7441 tacgatgtta tgggtggtac taggtaaaca tattggaatt agagaacgtc agttaattat
     7501 gttagataat aacaaaaaca caatgagtgg taccgtcaaa ttgattattg atattgtaaa
     7561 atcaatattt gtaatcgaac tcgtaggagc catgttatta gcattttact tttatcgaga
     7621 taatccagat ttaaaatatg caatcatgca aggtgttttt gtttctattt ctgccactac
     7681 caatggtgga ttagatatta caggtaagtc attaattcct tatgcacatg attattttgt
     7741 acaagcgata gttatatttt taataatttt aggatcaatc ggcttcccag tattattaga
     7801 agttaaagct tatattcaaa atagggttac taattttaga ttttcattat ttactaaaat
     7861 tacgacatca acatatttat tcctatttat tgttggggta ttagccattc tattatttga
     7921 acataaccat gcgttcaaag gattaagttg gcatcaatcg ttattctatt cgctgtttca
     7981 atcagcgact acaagaagtg cgggtcttca aacaattgat gtgacaacac taagtgaccc
     8041 cactaatatt atcatgggta ttttaatgtt tataggatct tcgccaagtt cggttggtgg
     8101 cggtattcgt acaacaactt tcgctatttt aattttgttt ttaattaact ttagtaataa
     8161 tgccgataaa acatccatta aagtttacaa tagagaagta cacattatgg atattcaacg
     8221 ttcatttgca gtatttacaa tggcgacaat tttaacattt ttaggaatgc taattatatc
     8281 agctactgaa aatggtaagc ttacattttt acaagtattt tttgaagtca tgtctgcatt
     8341 tggaacttgt ggactatcgc ttggtgtcac aagtgatatt agtgatattt ctaaggtcgt
     8401 actaatgata ctcatgttta taggacgtgt tggcttaata tcatttatca ttatgatagc
     8461 aggacgtcga gaaccagata aattccatta tccaaaagaa cgtattcaaa taggataata
     8521 taatagcaat ctaagtttag ttaatgtaga ttttaactgg aacttagatt gcttttttag
     8581 tttgtatttt taacttattt tataagacga ttggtttcga aaatggtaaa ctagtaacaa
     8641 tgagaggtgt aacatgatgg aaaaaaatga aaacattaat gtagagattt taactacgtc
     8701 agatatgcat agtcatttct taaatggtga ttatggttca aatatttata gagctggtac
     8761 ttatgttaac caagtaagag cacaaaatca tcgcgtcatt ttattagata gtggcggaag
     8821 tttagctggc tcgttagcgg cctattatta tgctattgtt gcaccttata aacgacatcc
     8881 aatgataaag ttaatgaaca gaatgcatta tgatgctagc ggtgtgagtc caagtgaatt
     8941 caagtttggt ttatcatttt taactcgttc aattgctttg gcacgttttc catggttatc
     9001 agcaaatatt gaatacaatg ttactaagga gccttatttt tcaactccat attgtattaa
     9061 acattttggt gacttaaaaa ttgctatcgt aggcgttaca gcagatggtt taatggaaaa
     9121 tgagtattct gaaatggagc aagatgtatc tattgaaaag acattagtgg catcaaaacg
     9181 atggattaga tatatccatg aagttgaaga gccggatttt ttgattgtaa tttatcatgg
     9241 tggattgaat aaaattagta atagtacgaa aaataaaaag gcaagttcga atgaagctga
     9301 aaaattaatg gaagaactcg gtgttataga tttaatgatt acagctcatc agcatcaaac
     9361 aatagtcggt caagatcatg aaacgtatta tgttcaggct ggtcaagatg caaaagagct
     9421 tgtacatctt tcgattaatt ttaaaaagag aacaacaact tatgatgttg aaagcattga
     9481 ttctaaagtg attgacttaa atgagtatga agaggaccaa gaattattag atttaacatt
     9541 ctatgataga aaagcagtgg cttattggtc acaggaaatc ataagtgata aaggtttgat
     9601 gttatcagta aatgggttac aagatttagt ctgtcaaaca catccatttt cacaattatt
     9661 acatgatgca attcaccttg catttgatga tgatataaca tgtgtccacg tgcctatgaa
     9721 cggagagaag gggttgagtg gacagattcg aaatgaagat ttgtatcatg catacccata
     9781 tccagataag ccaatggata tgacaattag tggtcaaaat atcaaagata tattggagta
     9841 tagttattca catttagatt ttgttaacga gcaattaagc ttaacaatta ttgatgaaac
     9901 gttatgtaca atgtggcaag gattcaatta tgagattgat atgaatcaag aacctgggca
     9961 acgagtaatg ttagatcaaa ttgatttgag taagagttat agagttacaa tgactgacta
    10021 ttgttatcgt aactacaaga attatttaaa aaatgctatt atacatgaat catacgatga
    10081 aacaatgagt acattaattg cagagaagtt aagagatccg aattatcata tttcatgtag
    10141 tgataatttt atagttaaaa acaggtaaat attgctatgt aataaattaa ttataagttc
    10201 aacaagtata aaaacctttc ttagagaaga ttatctagga aaggttttta gaattttaaa
    10261 attgatataa aagaacagtg aaattctcat tcattatata taagtaaaca aaaaagctaa
    10321 aatggtatat accattttag caagtaacgg aaacggaggg attcgaaccc tcgcgccgct
    10381 ttcgcgacct acacccttag caggggcgcc tcttcagcca acttgagtac gtttccaatg
    10441 gctccacagg taggactcga acctacgacc gatcggttaa cagccgatag ctctaccact
    10501 gagctactgt ggattaattt gtatgtgttt gaatatcaag cacaaatatt attataacaa
    10561 gattaagtta atttgcaata gataccttaa aaaaatatgc agaacattta cactatattt
    10621 ataaaacatt ccgcatattt taaaaat
//
LOCUS       DAMZLE010000089        10617 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMZLE010000089 DAMZLE010000000
VERSION     DAMZLE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.090"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            24..977
                     /locus_tag="POB92_001909"
     CDS             24..977
                     /locus_tag="POB92_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579391.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1140..1343)
                     /locus_tag="POB92_001910"
     CDS             complement(1140..1343)
                     /locus_tag="POB92_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDI6579392.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1642..2130
                     /locus_tag="POB92_001911"
     CDS             1642..2130
                     /locus_tag="POB92_001911"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6579393.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2123..3058
                     /locus_tag="POB92_001912"
     CDS             2123..3058
                     /locus_tag="POB92_001912"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI6579394.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERINWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3055..4362
                     /gene="rsmB"
                     /locus_tag="POB92_001913"
     CDS             3055..4362
                     /gene="rsmB"
                     /locus_tag="POB92_001913"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI6579395.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKFTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEKTETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4365..5459
                     /gene="rlmN"
                     /locus_tag="POB92_001914"
     CDS             4365..5459
                     /gene="rlmN"
                     /locus_tag="POB92_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDI6579396.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLYELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5466..6209
                     /locus_tag="POB92_001915"
     CDS             5466..6209
                     /locus_tag="POB92_001915"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDI6579397.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNRTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6206..8200
                     /gene="pknB"
                     /locus_tag="POB92_001916"
     CDS             6206..8200
                     /gene="pknB"
                     /locus_tag="POB92_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDI6579398.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8428..9303
                     /gene="rsgA"
                     /locus_tag="POB92_001917"
     CDS             8428..9303
                     /gene="rsgA"
                     /locus_tag="POB92_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDI6579399.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9304..9948
                     /locus_tag="POB92_001918"
     CDS             9304..9948
                     /locus_tag="POB92_001918"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI6579400.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9955..10596
                     /locus_tag="POB92_001919"
     CDS             9955..10596
                     /locus_tag="POB92_001919"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDI6579401.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
BASE COUNT         3964 a         1461 c         2006 g         3186 t
ORIGIN      
        1 aataacttaa gaggagaaag tttatgaaaa agacactggg atgtttactt ttaattatgc
       61 ttttagtcgt agcaggttgt tcttttggtg ggaatcataa attatcatca aagaaatcag
      121 aagaatcaaa acaagaaact gtaaaaaaag aatcggaaga agagaaagat ccagatttag
      181 agaaatatga agaaatagag aagaaaatga aaggaattaa agatgcgcca tctcttgata
      241 agttggatcc attaatgaca gaaaagtcgt ttacgaatag taaagggatt caaggatgga
      301 aagattacaa agaattaatg ggtaaagtgg aacttgcaga ttatagattt actaaagatt
      361 caaaaggatc ttcaataaaa gatgttgatg cattctttaa aggtaagaaa ggtataaaaa
      421 ggaaagtgat tgaaacacac gatgatgtaa aacaagttga ttattggtat gtagatccag
      481 atggaaagaa aattggcaat tcaaacacac ctgtttttta cgcagaaatt atgacaaaat
      541 ataaagatgg aaagttagtt tatgcatcag tcgaaccagg atcttacgta atacataaag
      601 atgatgcaat taaatatgac gattattcta agttaaaaaa attaagtcag ctaactaaac
      661 ttgatcatcc aaaaccagtt ccatatagcg tagctcaaat caaatctttc ggagtacctt
      721 taacaagcgt ttcatttatg acacatggat caaaggatac taaagatgaa gtgttgccgg
      781 cattggccta tttcactttt tcaccaaaaa attatgaaga caagtctaat ccagatccaa
      841 aagttttaaa tttagtacat atggatttct taaatgcatc tagtgatttt ggtaacgcac
      901 attttgttgt tttaagtaaa tatattaaag agtatgaatc aaactatgaa acagcgtcag
      961 atgattcttt aaaatagtat ttactgtgtg aaaaataaat agtgtactac attaaataat
     1021 cgcaataata atcccgataa acaatcagca ttactgctta tcatatagag ttcgtaataa
     1081 ctataactct atgattcgca aataataaat gattgtcatc gggatttatt tttatcaatt
     1141 tataaagtga cattaccttg ttcatcagca ggtttgaaaa cagtaatcac tgcactaata
     1201 attgctaaaa tgtgtgggat acctgtccaa cagaatatta agtgtagaat accttgcata
     1261 ttcttgccgg cataaaattt atgaatacca aaactaccta agaacaatgc taataaaata
     1321 taaataactt tgtttacttg catttctttc cctccagttg aattgcttat aatgacatta
     1381 gcttctcttt ttattatacc cacttttagt tcaaacattc tagtttaagc attcccaatc
     1441 atctaaattt cagttattca atccttacaa taaatttagg attacacttc agttgcattg
     1501 tattatttta cgtgtgaaat atacgtaatg aatcacatga caatctttaa attgaaaaat
     1561 atacattcta tgatgtaagg tcgcattttt aatatattta cgttataatg gtttgatgaa
     1621 ttttattgag gagtaagagc tatggcgatt aaaaagttag taccagcatc gcatcctatt
     1681 ttaacgaaaa aagcgcaagc agttataaaa tttgatgatt cgttaaaaag attattacaa
     1741 gatttagaag atacaatgta tgcacaagaa gcagctggct tatgtgcacc tcaaattaat
     1801 cagtcattgc aagtggcaat cattgatatg gaaatggaag gattattaca acttgttaat
     1861 ccgaaaatta ttagtcaatc aaatgaaacg ataacagact tagaaggttc aattacattg
     1921 ccagatgttt acggcgaagt gacaagaagt aaaatgatag ttgtcgaaag ttatgacgtc
     1981 aatgggaaca aagttgaact aactgcacat gaagatgtag caagaatgat tttgcatatt
     2041 atagatcaaa tgaacggtat cccttttaca gaacgtgcgg accgtatttt aacagataaa
     2101 gaagtggagg catattttat aaatgactaa aataatattt atgggtacac cagacttttc
     2161 aacaactgtt ttagaaatgc ttattgcaga acatgatgtc attgcagtcg taacgcaacc
     2221 agatcgacct gttggacgta aacgtgttat gacaccacca ccagttaaaa aagttgcaat
     2281 gaaatatgat ttacctgtat atcagcctga aaaattaagt ggatcagaag aattagaaca
     2341 attgcttcaa ttagatgtag atttaattgt aactgctgct tttggacaat tattacctga
     2401 atcattgttg gcattaccaa aacttggggc aattaatgta catgcatcat tgttaccgaa
     2461 gtatagaggt ggtgcaccaa ttcatcaggc aattatcgat ggtgaacaag aaaccggcat
     2521 aacaattatg tatatggtta aaaaattaga tgcgggtaat attatttcgc aacaagcaat
     2581 taaaatagaa gaaaatgata atgtcggtac gatgcatgat aaattaagtg tattaggggc
     2641 agatttatta aaagaaactt taccatctat tatagagggc acaaatgaaa gtgtacctca
     2701 agatgatacg caagcaacat ttgcttccaa tattcgacgc gaagatgagc gaattaactg
     2761 gaataaacca ggaagacaag tgtttaatca aattcgtgga ttatcaccat ggccagttgc
     2821 ttatacaact atggatgaca ctaacttgaa aatatacgat gctgaactcg ttgagactaa
     2881 taagataaac gagcctggaa ccattataga aacgactaaa aaagccatta ttgttgctac
     2941 aaatgataat gaagctgttg caattaaaga tatgcaatta gctgggaaaa agagaatgtt
     3001 agctgccaat tatttaagtg gtgcgcaaaa cacactagta gggaagaaac ttatatgata
     3061 gaaaacgtga gaagtcttgc ttttgacacg attcaagata tattaaatga aggtgcgtat
     3121 agtaacttgc gtatcaatga agtgttgtca gaaaatgaat taaatgcaat ggataaggct
     3181 ttatttacag aaattgtcta cggaaccgtt aaaagaaaat ttacgttaga tttttattta
     3241 aagccttttg tgaaaacaaa aattaaggca tgggttaggc aattattatg gatgagtatt
     3301 tatcaatatg tttatttaga taaagttcca aatcacgcca tcattaatga agcagttgaa
     3361 atagcaaaag aacgcggtgg ctatcataat ggtaatgtcg taaatggtat tttacgtaca
     3421 atgatgcgta gtgacttacc tgattttaat gaaattgcag atcctaaaaa aagaatggca
     3481 atcgagtata gtatgccgaa gtggattata gatcattggg caacacatta tggtctcgaa
     3541 aaaactgaaa caattttaca gtcattttta gaaacgacat caacaactgt gcgtgccaac
     3601 ctgacgcgag catcattaga tgatattatt gaaaagttgc aagacgaagg ttatgacgtt
     3661 gaaaaagatc atgacttacc ttattgtctc catataggag gacaaccaat tattcattct
     3721 cgttcattta aagatggatt cgtttcaatt caagataaaa gctcaatgtt tgttgcacac
     3781 attatgaatg tagaccgaca tgatcacgta ttagatgcat gtagtgcacc tggcggtaaa
     3841 gcttgtcaca ttgctgaagt tttaatgcca gaagggcaag ttgacgcttc agatatacat
     3901 gatcacaaaa tagacttaat taattttaat ataaaaaaat tacgattaac aaatattaaa
     3961 gcttttcaac atgatgcgac aaaaccttat gataaaacat acgataagat acttgttgat
     4021 gcaccatgta gcggattagg tgtaatgaga cataagccgg agattaagta tactcaaagc
     4081 aaacaacata ttgagtcact agttgaatta cagcttgaaa tattggaaaa tgtaaaaaac
     4141 aatgtaaaaa taggtggcga aatcatctat tcaacatgta caattgagca actagaaaat
     4201 gaaaacgtga tttatacgtt tttgaaaaat aataaaaact tcgaatttga accgtttcaa
     4261 catccgataa ctggagagtt ggtcaaaacg ttacaaatca tgccgcaaga ctttaattca
     4321 gatggattct ttatcactaa gataaaaaga aaggacaatt aggaatgata actgctgaaa
     4381 agaaaaagaa gaataaattt cttccaaatt ttgacaagca atcaatatat tcattgcgat
     4441 ttgacgaaat gcaaaactgg ctcgttgaac aaggtcaaca aaaatttcga gcgaaacaga
     4501 tttttgaatg gttatatcaa aaaagagtag attcgattga tgaaatgacg aacttatcga
     4561 aagacttacg acagctttta aaagataact ttactgttac aactttaaca actgtagtaa
     4621 aacaagaaag taaagacggt acaattaaat tcttatatga attacaagat ggctatacaa
     4681 ttgaaactgt tttaatgaga catgattatg gaaattcagt atgtgtaacg acacaagtag
     4741 gttgtcgtat cggatgtacg ttttgtgctt ctacacttgg cggcttaaaa agaaaccttg
     4801 aagctggcga aattgtttca caagttttaa cagttcaaaa agcccttgat gctacagaag
     4861 agcgcgtatc tcaaattgtc ataatgggta tcggtgaacc atttgaaaat tatgatgaaa
     4921 tgatggactt tttaagaatc gtcaatgatg ataatagttt aaatattggt gcacgtcaca
     4981 ttacagtatc aacatcaggt atcattccta gaatatacga ctttgcggat gaagatatcc
     5041 aaattaattt tgctgtaagc ttacacgccg caaaagatga agtgcgatca cgcttgatgc
     5101 caattaaccg tgcatataat gttgagaagt taatcgaagc aattcaatat tatcaagaaa
     5161 aaacaaatcg tcgtgttact tttgaatatg gtctgtttgg tggtgtgaat gaccaactag
     5221 aacatgcaag agaattagca catttaataa aaggcttaaa ctgccatgtt aacttaattc
     5281 ctgtcaacca tgttccagaa agaaattatg tgaaaacggc taaaaatgat atctttaaat
     5341 ttgaaaaaga attaaagaga ctaggaatta atgccacaat acgtcgtgaa caaggttcgg
     5401 atattgacgc agcttgtggt caattaagag caaaggaacg acaagtagaa acgaggtaaa
     5461 gacaaatgct agaggcacaa ttttttactg atactggaca acatagagat aagaatgaag
     5521 atgcgggtgg tattttttat aatcgaacta atcaacaact tttagttctg tgtgatggta
     5581 tgggcggcca taaagcagga gaagttgcaa gtaaatttgt tacagatgag ttgaaatccc
     5641 gttttgaagc ggaaaatctt atagaagaac atcaagctga aaattggttg cgtaataata
     5701 taaaagatat aaattttcag ttatatcact atgcacaaga aaatgcagaa tataaaggta
     5761 tgggtacaac atgtgtttgt gcacttgttt ttgaaaaatc agttgtgata gcaaatgtcg
     5821 gtgattctag agcctatgtt attaatagta gacaaattga acaaattact agtgatcact
     5881 catttgttaa tcatcttgtt ttaacgggtc aaattacgcc ggaagaagca tttacacatc
     5941 cacaacgtaa tattattacg aaggtgatgg gcacagataa acgtgtgagt ccagatttgt
     6001 ttattaagcg attaaatttt tatgattatt tattattaaa ttcagatgga ttaactgatt
     6061 atgttaaaga caatgaaatt aagcgtttgt tagtaaaaga aggtacaata gaagatcatg
     6121 gtgatcaatt aatgcaattg gcattagata accattcgaa agataacgtt actttcatac
     6181 tcgcggctat tgaaggtgat aaagtatgat aggtaaaata ataaatgaac gatataaaat
     6241 tgtagataag cttggcggcg gtggcatgag taccgtttat cttgctgaag atacgatact
     6301 taacattaaa gttgcaatta aggcgatttt tataccacct agagaaaaag aagaaacatt
     6361 aaaacgtttt gaacgagaag tacataactc atcacagcta tcacatcaaa atatagtaag
     6421 tatgatcgat gttgatgaag aagatgactg ttactactta gtaatggaat atattgaagg
     6481 tccgactttg tctgagtata ttgaaagtca tgggccatta agtgttgaca cggcgattaa
     6541 ttttacgaat caaatattgg atggtattaa acatgcgcat gatatgcgta ttgtacatag
     6601 agatattaag ccacaaaata tattaattga cagcaataaa acgttgaaaa tatttgattt
     6661 tggaattgct aaagctttaa gtgagacgtc tttaactcag actaatcatg tgttaggtac
     6721 tgtgcagtac ttttcgccag aacaagcaaa aggtgaggca acggatgaat gtacagatat
     6781 ttattctata ggtattgtgt tatatgaaat gcttgttggt gaaccaccct ttaatggaga
     6841 aactgcagtt agcattgcga ttaaacatat tcaggattct gtgccaaatg tgacaacaga
     6901 tgtacgtaag gatattccgc aatctttaag taatgtcatt ttacgcgcta cagaaaaaga
     6961 caaagcgaat cgttacaaaa caattcaaga aatgaaagat gatttgagta gtgttttaca
     7021 tgaaaatcga gcgaatgaag atgtctatga actcgataaa atgaaaacga tagcggtacc
     7081 tttgaaaaaa gaagatctag caaagcatat tagtgaacat aagtcgaatc aacctaaacg
     7141 tgaaacgacg caagtaccta ttgtaaatgg gcctgctcat catcagcaat tccaaaagcc
     7201 agaaggtacg gtttacgaac caaaacctaa aaagaaatca acacgaaaga ttgtgctctt
     7261 atcactaatc ttttcgttgt taatgattgc acttgtttct tttgtggcaa tggcaatgtt
     7321 tggtaataaa tacgaagaga cacctgatgt aatcgggaaa tctgtaaaag aagcagagca
     7381 aatattcaat aaaaacaacc tgaaattggg taaaatttct agaagttata gtgataaata
     7441 tcctgaaaat gaaattatta agacaactcc taatacaggt gaacgtgttg aacgtggtga
     7501 cagtgttgat gttgttatat caaaaggccc tgaaaaggtt aaaatgccaa atgtcattgg
     7561 tttacctaag gaggaagcct tgcagaaatt aaaatcgtta ggtcttaaag atgttacgat
     7621 tgaaaaagta tataataatc aagcgccaaa aggatacatt gcaaatcaaa gtgtaaccgc
     7681 aaatactgaa atcgctattc atgattctaa tattaaacta tatgaatctt taggcattaa
     7741 gcaagtttat gtagaagact ttgagcataa atcctttagc aaagctaaaa aagccttaga
     7801 agaaaaaggg tttaaagttg aaagtaagga agagtatagt gacgatattg atgagggtga
     7861 tgtgatttct caatctccta aaggaaaatc agtagatgag gggtcaacga tttcatttgt
     7921 tgtttctaaa ggtaaaaaaa gtgactcatc agatgtcaaa acgacaactg aatcggtaga
     7981 tgtaccatac actggtaaaa atgataagtc acaaaaagtt aaagtttata ttaaagataa
     8041 agataatgac ggttcaactg aaaaaggtag tttcgacatt actagtgatc aacgtataga
     8101 cattccttta agaattgaaa aaggaaaaac agcaagttat attgttaaag ttgacggtaa
     8161 aactgtagct gaaaaagaag tcagctatga tgatgtataa atataattga agtaaatgta
     8221 ccgaggtttc tatttggaag tctcggtatt tttatgttga gattgcggta gttttaaaat
     8281 gcttcatgtc atcatataca ggtgagcatt gtctatcaat tctgtatcac tctcacaata
     8341 tttatatcat ttgaaatgat catttatata ttaattttcc agtctttgat ataataaatg
     8401 cagtaaaatt aatcgagagg tgccattttg aagacaggtc gaatagtgaa atcaattagt
     8461 ggggtatatc aagtagacgt taatggcgaa cgtttcaata caaaaccacg aggattattt
     8521 agaaagaaaa aattttcacc ggtagttggt gatatagtgg aatttgaagt acaaaacatt
     8581 aacgaaggct atattcatca agtgtttgag cggaaaaatg agttgaaaag accacctgta
     8641 agtaatatag atacactagt aattgtaatg agtgctgtcg agccaaattt ttcaacgcaa
     8701 ttattagatc gatttttagt tattgcacat tcgtatcagt taaatgcgag agttttggtg
     8761 actaaaaaag ataaaacacc aattgaaaag cagttcgaaa ttaatgagtt gttgaaaata
     8821 tatgaaaata ttggctatga gactgaattt attggaaatg atgatgatcg aaaaaaaatt
     8881 gtagaagctt ggccagctgg acttatagta cttagtggtc aatcaggtgt cggtaagtcc
     8941 actttcttaa atcattatcg tccagaactt aatcttgaga caaatgatat atcaaaatca
     9001 ttaaatcgag gaaagcatac tacaagacat gtcgaactat tcgaacgtca aaacggttat
     9061 attgcagaca cacctggatt cagtgcttta gattttgatc atatagataa agatgaaata
     9121 aaagattatt ttcttgaatt aaatcgatat ggtgaaacat gtaagtttag gaattgtaat
     9181 catatcaaag aacctaattg taatgttaag catcaattag agatagggaa tattgcgcaa
     9241 tttagatacg accattattt acaactattt aatgaaattt caaatagaaa ggttagatat
     9301 taaatgacaa aactatatcc atcattatta tctgttgatt ttttggattt acaacatgaa
     9361 ttaaaacgac ttgaagaagc aggtgtcgac ggagttcatt ttgatgttat ggatggtcaa
     9421 tttgtgccta atatatctat tggtttacca atattagatg cagtaagaaa aggcacaaca
     9481 ttacctatag acgtacattt gatgattgaa aatccagaaa agtatattgc atcatttgca
     9541 gaacatggtg ccgatatgat ttcaattcat gtcgaatcaa cgcctcatat tcatcgtgct
     9601 attcaaatga ttaaacattt agataaaaaa gctggtgtag taattaatcc tggtacacca
     9661 atatcacaaa ttgaacctat tttagacatt gttgattatg tactagtgat gacagttaac
     9721 ccagggtttg gtggtcaatc atttattgat caatgcgtag aaaaaatagc gggtcttaat
     9781 gctattaaaa tggaacgtca attaaacttt gatattgaag ttgatggagg cgtaaatacc
     9841 gatacagcga aagtttgtgt tgaaaatggt gctacaatgc tagtaacagg ttcatttttc
     9901 tttaaacaag aggattataa aaaagtcaca caagcattga aaggttgagt gtatatgcat
     9961 ataaatttat tatgttctga tcgacacttg ccgcaagata tttgggccaa aagtaatgaa
    10021 ggtaaatggg gcggcgttga tagaggtgct ttgattttat tgaagcatca aattatccct
    10081 tttttctcag tgggagactt tgattcagtc agtaaagaag aacgccaact tctaacagaa
    10141 cagttacaaa tcaaaccagt tcaagctgaa aaagctgata cggatttagc tttagcggtt
    10201 gataaagctg ttgcacttgg atttgatagt attacaattt atggtgcaac aggcggacga
    10261 ttagatcact tttttggggc aattcagtta ttattgaaaa aagcatatta taaacatgat
    10321 gttcatatag aagttatcga tcaacaaaat aaaattgaat tattgcctaa aggtcaacat
    10381 acagttgaaa aagataagag ttatccgtac atttcattta taccgatgac tgatgatgta
    10441 gaactttctc tagcaggttt taaatataat ttagctagac aaatgcttaa tataggttct
    10501 actttaacta tttcaaatga aattgagtct ttgcaagcga aagtaactgt acatgatggg
    10561 ttgattttgc aaattagaag tacagattta aattaagtat attcaaattt gtgagga
//
LOCUS       DAMZLE010000090        10098 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMZLE010000090 DAMZLE010000000
VERSION     DAMZLE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.091"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            27..920
                     /locus_tag="POB92_001920"
     CDS             27..920
                     /locus_tag="POB92_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6579402.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            1251..2774
                     /locus_tag="POB92_001921"
     CDS             1251..2774
                     /locus_tag="POB92_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579403.1"
                     /translation="MSKKLKIIIPIIVVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            2842..4008
                     /locus_tag="POB92_001922"
     CDS             2842..4008
                     /locus_tag="POB92_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579404.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALVITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRVLITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            4005..4925
                     /locus_tag="POB92_001923"
     CDS             4005..4925
                     /locus_tag="POB92_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579405.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQQSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLTRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            4939..5598
                     /locus_tag="POB92_001924"
     CDS             4939..5598
                     /locus_tag="POB92_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579406.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENINFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(5650..6600)
                     /gene="lytM"
                     /locus_tag="POB92_001925"
     CDS             complement(5650..6600)
                     /gene="lytM"
                     /locus_tag="POB92_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDI6579407.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPAIAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGLASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(6926..7918)
                     /locus_tag="POB92_001926"
     CDS             complement(6926..7918)
                     /locus_tag="POB92_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDI6579408.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKAGAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            8152..9528
                     /locus_tag="POB92_001927"
     CDS             8152..9528
                     /locus_tag="POB92_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579409.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(9656..10048)
                     /locus_tag="POB92_001928"
     CDS             complement(9656..10048)
                     /locus_tag="POB92_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579410.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT         3433 a         1754 c         1585 g         3326 t
ORIGIN      
        1 aatacataaa ggattaaatg gttattatga agaaaacaat tttactgacg atgacaactc
       61 ttactttatt tagtatgtcg cctaactcgg ctcaagcata tacgaatgat agcaaaacat
      121 tagaagaagc aaagaaagca cacccaaacg cacagttcaa agtgaataaa gacaccggcg
      181 cgtatactta tacatatgac aaaaacaaca cgccaaacaa caatcatcaa aaccagtcac
      241 gtacaaacga caatcatcaa cacgcaaatc aacgtgatct taacaacaat cagtaccatt
      301 cttcattaag tggtcagtat acgcacatta atgacgcaat tgattcacac acaccgcctc
      361 aaacgtcacc aagcaatcct ttgacaccag caataccgaa tgtcgaagac aatgacgatg
      421 aattaaataa cgctttttca aaagataaca aagggcttat tacaggcatc gatttagacg
      481 aattgtatga cgaattacaa atcgccgaat ttaatgacaa agcaaagacc gctgacggta
      541 aacctttagc attaggtaac ggtaaaatca ttgatcagcc tcttatcaca agtaagaaca
      601 acttatatac tgctggacaa tgtacatggt atgtctttga taaacgtgcc aaagatggac
      661 acacgattag tacattttgg ggagatgcta aaaactgggc aggccaagct tcaagcaatg
      721 gcttcaaagt agatagacac ccaacacgag gatcaatttt acaaacagta aatggtccat
      781 ttggtcatgt agcctacgtt gaaaaagtta atattgatgg aagtattcta atttcagaaa
      841 tgaactggat tggtgaatat atcgtttcat caagaaccat ctctgcttca gaagtttcat
      901 catataatta catccattaa attaatcatg acatcaataa aaagcgacca gttcgcagtt
      961 tacaattcgt aacactgcaa aattggtcgc tttattttgt atgttattcg attataaaat
     1021 atgatgtcta ccttcaaaat tgcaataact tgtcacctta aagttaaccg acatttttaa
     1081 ataatcatta tgtaatcaaa atattttttg tagtttatta gatttccaat aacttattaa
     1141 acattattat aactaaaatc ctattaattt aaacaaaatt taaaataaac catttaataa
     1201 aatgtattgt tctgatatga ttaactcata aataattggg aggaaacaaa atgtctaaaa
     1261 aactaaaaat tataattcct attattgttg tcttattatt aataggtgga atcgcatggg
     1321 gagtttatgc attttttgct aatacaccga aaaatacata cctaaaaagt gaacaacaaa
     1381 cagcaaaaat gtataaagat tattttaatg accgttttga aaatgaagtg aagttccaag
     1441 aaaagatgaa agataattca tttttatctt cattagaatt aagcgcagat gcatcagatg
     1501 aaattgttaa ggggcttggt attcctaaat ctgttgttaa tgcttcgaaa attaaaatgt
     1561 catatggaca tgatcctaaa aaagagaaat caatgattaa tcttgaacca acaatagcag
     1621 actctgcatt agggaaattc cagttagctg cagataaaga taagcattat ttcgaatcac
     1681 cattatttaa agggaaatat agtgttaata attctgattt attatcaact tattcaaaac
     1741 ttacaggtga agatgaagaa acagcaaaag aaaatggtat tacaaaccaa caactaaatt
     1801 taaatactct ttttagtaat gctcaagcac aacaaagtga ctacagcaaa attgccgaaa
     1861 aatattccga acttattgtc gacaaattag atgacgataa ttttgataaa ggtaaaaaag
     1921 aagaaattaa ggttaatggt gaaaagtaca aagttagacc tgtcacgtta acacttagca
     1981 gagctgacac taaaaaaatt acattagctg tattagaaga agctaaaaag gataaagacc
     2041 ttaaaaaatt aatggaagaa caaggtgcta caaaagactt tgaaaaagac atcaaaaaag
     2101 caattgacga tgtcaaagaa actaaaaagg atgaatttgc taaaattcaa tctaaaattt
     2161 ataccgaaaa acatacgatt gtaaaacgag aaattactat tacagacaaa gaaaataata
     2221 aaactaaaat caaaggtact aatactttag aagacgataa gttaaaacta gattacgcac
     2281 ttgatttcga tcaagataaa tacacgtatg ctgaagcgaa atatacaatt aaaggcgtat
     2341 cttctaagga aaaagacaat aaatacagtg ataaatacga atttggtaaa aagacagaat
     2401 atgatgaatc aaaaatcaaa ttagataacc aagaaaaagt agatggcaca aaacgtcaag
     2461 ataaaggtaa aattactgtc gcattagata aatatagcga cgaaaatgaa ttcacttttg
     2521 aaaataatat agattctgac gtcaaaaata acactcagaa atctacgtta aatatcggca
     2581 tcaaatatgc tgaagaacca attaatttca ttttaaaatc tagcacaaaa ttgaaagcag
     2641 atattgattt tgatgatagt ggtgcgaaag atttcaatag tctatcttca aaagaccgtg
     2701 aaaaacttga aaaagaaatc gaaaaaaatg gcggcaaaat gtttgaatca attttaaaaa
     2761 aggcatctaa ataactaaaa tataaaaata agattggaag gtcatcttgc ctttcaatct
     2821 tatcattgga ggagtggtta aatgaaaaca atacatttgt ttcgcatcta ccactctttt
     2881 ttattgaaaa agtggtattt gattatctat ttactattta tattagcggc actcgttatt
     2941 acattaacga cgatccaaca tgtaacagaa gatgacaatc attttaatat aggtgtcgta
     3001 gataaagatc aatcaagtga aacgaaatta atcttaaact ctattggtaa agggagtaac
     3061 ctaggaaaaa acgtgagcat taaggcatat gatgataagc aagcacatac tttgttaaaa
     3121 aaacataaac ttcaaggcta ttttgttttt gataaaggta tgaccaaggc attttataaa
     3181 caaggcgaac taccaatttc agtatataca tatgatcaac aatccatgaa aagtgtcgtg
     3241 ctatctcagc taacagattc tgtttaccaa cgtcttatgc gatcaatggg tggcatctta
     3301 gcttttcaag acttagcacc gaaagcatca cattctgaca gtatcaatgt tatgactgat
     3361 ttgctgatta caggattaaa ccgttcaggt gcatttaact tagaaccgat tcatttatac
     3421 gatacgggca gttattatgc aattacaggt tttttaacaa cggtattcat ctttgcacta
     3481 tctttattta cagttttgaa aatgaatcaa gatactgtat tgaaagcgcg attgaaaatg
     3541 tttcattttt ctaaagagcg tttattaatc attcgtgtgt tgattacatg gttttatact
     3601 atgttatgga gtatcgttgg tgtagtttgg attgtgttca gtattccgaa tacctttgaa
     3661 ttatataatt ggccaacgtt agccattcat ttaagctatt atgttacctt tttaatactg
     3721 tggttattac tcatcgaact tttaacaaca ggcttgttga atagtattag taaagtcata
     3781 ttagctattg tcatcctggt gttatctggt ttaacaatac ctacgatttt tttacaacat
     3841 atagcaaatg gcgttttcaa tattcaaccg ttcgcagttg taacaaatca attgttagaa
     3901 attattttaa acaactacat tttagaactg caccctagtt tctatcttag tttcatcgca
     3961 cttttgataa ttaatttagc tgtattggtt tggaggtatc gtcaatgaag ccttatatac
     4021 aacttgttgt gttcaagcaa tggttacaat acatcttgct cgtaacaacc attgtcatcg
     4081 cactcgtact tattggtatc ggttaccgtg tagcacatga caacttcaaa ataccgatta
     4141 ccattcaaga tttagaccaa accactgcat caaaatcatt cgtcaataaa attaaacaat
     4201 ctgactatgt aactattaaa aaagtcgatg aagatgaaag ctatattgaa gatgatgtta
     4261 ctaaaaagga agctatttta agtatgcaaa ttcctaaagg tttctctcaa aaattaaaag
     4321 agaaccgttt aaaagaaacg atacagttat atggtagaga tgactttata ggtggtattg
     4381 ctgtagaaat tgttagtagt tcattatatg aacagcaaat tcctaacatt atttatgaac
     4441 accttgagga tatgaaacaa catcaatcca tcgatgctat caacaagtcc tatcataaac
     4501 atacacctga atctaaaatc aaatttgtgt cgcttactaa acaagcacaa caatctattt
     4561 caattagctt aatctttgct gtgattttat ttgttagcgc tgttcaagta gtacttcatt
     4621 atcgtttaaa ccaacaagca gcattgcaac gattatcaca atatcattta acccgtttca
     4681 aactatatag tacttatgta atgacacata cgatcttgtt attgttggta ctattggcag
     4741 ttagtctata tttgtctcaa ccactcagct taatatttta cttaaaatca ctgttactta
     4801 tattgattta tgagataggt atcgtcttta tcttattcca tattcaaaca ataagtcatc
     4861 gattattcat gacatttata tatgcacttg ctatgggtat cgtatacttg attattttca
     4921 tgtaaaggag cgtaactgat gatagaaatt aataaccttt caaagcgtta ccgtaacaaa
     4981 cagattttca atcatttaac catgtccttt gatagtaatc gtttaaccgt attacttggt
     5041 gataatggcg ctggaaaatc aacattactt cgtatgatcg ctggtattga aaaagctaat
     5101 gatggaacta tcaactattt cggcgaaaaa tggaatcaaa gacaaatcca aaatcacatc
     5161 ggttatgtgc cacaagacat tgcgttattt gaacacatga cagtggctga aaacattaat
     5221 ttttttaaat cactttgtaa aaatccaatt aacgatacaa ctatcaacga atatttacag
     5281 caattaaatt ttgatgatac gtctgccaaa gtatctacat tgtccggtgg gaataaacgt
     5341 aaaattaata tattagtagg tttactaggt caacctcgaa ttctaatttt agatgaaccg
     5401 acagttggta ttgatttgaa atctagacat gacatccacc aactgcttaa cattatgaaa
     5461 tctaaatgtt taattatatt aactacccat catttagatg aagttgaagc acttgcagat
     5521 gatatcaagt taattggcca agatcctttt tatcaacatg ttttagagga caagcaatgg
     5581 gcttatacct attattaaaa cgaaaaaatc ccaagttgcg tatgatatcg taacttggga
     5641 tttactgtat tatctacttt gcaagtatga cgttgggtct actgcatatt gattaccgat
     5701 gccaccagac atacgttgga agtgtacgtg aggcgctgtt gaattacccg tactacctga
     5761 atatgcaatt tggtcaccag ctttgacttt atcaccagct gaaacagtta aacgattatt
     5821 atgcatatac cattggtagt tattactgtt cgcttcttta atagttactt gattgccgcc
     5881 accatagtta ctccaacctg cttgtactac tgtgccatca gttaatgagt aaactggtga
     5941 attttcaggc attgcatagt cgacaccgta atgcgcacca ccaccgtgat attgtccata
     6001 tggttgtagt tgtttacgac ttgttaacca gcttgcgtct ttcgcatgac cactagctgt
     6061 cgctttactt gctaatccgc cattttgatt cgatgtgcca ttagggtaat tgacctttcc
     6121 attaccatca tggctgttat acgcttggtt gttgttacta tgtgaataat agctcgcgtc
     6181 tggacctaca tttgattgat aaccatattg attaatatgc tgttggcttt gactcgctgt
     6241 gtagtcattg ttatctcctg ctattgctgg attcacatat gtttggccgc ttccattggc
     6301 atttgcattt tttggataac agttataaaa ataatgcgta tgtccttgag catctacgaa
     6361 tgtatagcta tattctttat tatcaaacat tgcttgattc cagttaccat caggtgtgtg
     6421 atgataatcc ccattagaat caattgtata ataagtacca taagatacgt cttgtgattg
     6481 tgttgacatt tgtgtatgtg cttgttgggt gtttgtcgtt tctgctgcat ctgcttgatg
     6541 cgccattgta aatgtagcga agcccatcgt tgcaatcgct gctgctgtta attttttcat
     6601 gtataaaaca tcctccatta aaattaaagt tagttttcaa ttaaactgta ctgcacatac
     6661 taaaagaatt agacaactga gtaaaggatt taattctcat tttccaacta tttaatattc
     6721 ccgaaatgtt ttactaaact cattacattg tcattacaaa atagccatac attgatatta
     6781 aaatgacatc tctcactgca ttcgtttaac cttttataaa ttttcaaaac taacaactaa
     6841 tcgttcgtcc atgttcgcga ttcaacgcta atgtatgata gtaatcatcc atcaaatcat
     6901 atcaaccaaa ttccattatc aatcgctatt gattgtcatt caactttcta atagtgatat
     6961 gcttctcagg cttaaaaatc gtcatatctt ttctattaat taaatcatct gtgagcttta
     7021 atgctactaa ttcattgctg ccataatact taatatataa cgttcttgta gttaaattta
     7081 ttacggtctg atacatcgta tagtgattgt cagcatcatg cggacgtaca attccaatcg
     7141 gtatatttac cgcatctaat aaataaaatg cattcattaa atccatttct ttatcattgt
     7201 tttgagcaat gtttgctttc ataaatgcca ttctcacaaa gcgctcagtt gaagtaaatc
     7261 cacctggcaa tccaaatgta cctgcttcat tgcctaaagg ttcaatcgtt acaccttcca
     7321 ataactttgc tgttgctgga taaggagaaa tattgatata ttgtcttaaa ttactataat
     7381 gccaatctaa gtctggatga tttgttaaga caccaatagg gttatctttt ataaccactt
     7441 caccctcttt aaatgaaact tcgactgaat gtccagttgc atcggaaaca tgataatgca
     7501 atggcggaac ttcaccgatg tcatttaaat ataccgctac aacatgtatt tgggatgctt
     7561 gttgtttcat atcttcaatg cttgttgtat atcccaaaat ccatgtcaca atttcatttt
     7621 gcgtaatatt catcgcgccc gctttgtgtg ttgatccata tgaactataa cctcggaaat
     7681 attgtgttga aatggcaacg ccatgttcat taacaccatc accataaata aaaccttcca
     7741 tatctgttcc tgtaccaata aagccatatt gcgtttggcc tgtcgtgcca gtgcgagatg
     7801 tccaacgata atttctaggc gtcactgctg gcgaaccatc taatggataa tcataatcca
     7861 tcgtgcgtcc aagaagtact tgattattta aagtttgtat tgtgaatcct gtgcacattg
     7921 ttctcactcc tctgtacctt catttacttt aatcactttc aaataaagct gtttcactta
     7981 aacatactat aaaaattcaa tcatacaagc aattaattga tattcattct caataactgt
     8041 ggtatgatat gtaaggaaat catgactaat gtgtgagtga acgatcatct atacatccgt
     8101 tcacttcatc tcatgacttt ctatatttaa tttttacaag gagtgacatc tgtgaataac
     8161 acacaatctt caccacgcag taatattatt attgcgatta tgttgtctgc attaacatat
     8221 tggttgtttg cacaatcatt tattaatata ggacctctcg ttggtcaaac gtatcaaacc
     8281 tctcctgccg tgttaaattt atctattagt ttaacttcct tcgccacagg tatcttcatg
     8341 gtggctgcag gtgatattgc tgataaaata ggacaactga gaatgacata catgggtctc
     8401 ataatcagta tgtttgcatc tcttctatta attatatcgg acatcactgc actgctcatc
     8461 atcggtagaa ttttacaagg tctatcagca gctatcttgt taccttcaac agttggcgtt
     8521 ttaaataatc aatttaaagg agaacattta agacgagcga ttagttatct aatgattagt
     8581 actgttgggg gcatcggcct agctggtgtt atcggcggtt taattgccac aaatttcgga
     8641 tggcaaacga atttcatcat tagtatagtc attgctttca ttgccatatt acttctaaaa
     8701 ggcacacctg aaaaagtaag tcaacatagc catcgtcatc cattcgatta caaaggtatg
     8761 tcgattttcg ctgttatgat tggtagcttt acattattgt taacacaagg attcgaacaa
     8821 ggttggttta gtacattttc attcatttgt ctgagcattt ttatcatcac tacgttgata
     8881 ttcatcatca tcgaacgtcg acatgaagta ccttttattg atttctcagt attacgcaac
     8941 cgtccgttca ttggtgcatt tttaaataac tttgttttaa atagtggcct aggcgtaaca
     9001 gtggtctttt tcatatatgc tcaaacacac cttggtttat cagctgcgca atctggactt
     9061 gttacattgc catatgccat tgtggcagtt gcgatgattc gtttaggtga aaaagcaaca
     9121 ttacgtttcg gtggcaaatt gatgctcatc attggtccgt tgttccctgt catcggcatc
     9181 actattatta gcatgactca attatcggca tcacaatatg tcattgcagt tatcattggt
     9241 ttcgtcattt gtgcaattgg aaatggttta gtcgcaacac ccggccttac aattgcaatt
     9301 ttcagtatgc caaatgaaaa agttggttta gctacaggat tatataaaat gagtggtaca
     9361 ttaggtggcg cctttggtat agcactaagt actacagttt tcagtatgtt acaactaaac
     9421 tatgcaccaa gtgtagctgc aaccgtaaca tttatcgtca gcattgtatt gatgatcctt
     9481 ggctcattgt ctgcatacat gatcattcca aaaacggtta aatcttaaat ataatagaag
     9541 catcatgttt cgaaatatct gtatcacttt aaaatgatat acaagaaatt caagaaaaat
     9601 aagcgaagaa cgtttttctc tgttcttcgc ttactctaat tatattcact atcaattaga
     9661 caaaatggcc attttcaaat atcacgcgtt gtttctgacc ttgaatatat ttattataat
     9721 tctctttttg aaaatcagtt aacattaatt tagatgtacc gtattttaac actttttgca
     9781 ttgtttctat tctcattttt ctaaataacc atccatcttt taacacaata cgattaacag
     9841 catcatatga taattctact gtttctttaa tttcaaatgt cttgaatgaa ataatcgtgc
     9901 acattaaaaa cgtatcacca aagtaataaa catctaaatc atcacgttta tgttgtccaa
     9961 ctaacaaacg accatattcg aactcttttt ctggatattt caattctaaa aaactaataa
    10021 tctcttcttc ttttaatttg aattgcattt aaaaacatcc tctcttatgt tttaacaagc
    10081 cttaattaaa aatttttt
//
LOCUS       DAMZLE010000091         9701 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMZLE010000091 DAMZLE010000000
VERSION     DAMZLE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.092"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(15..90)
                     /locus_tag="POB92_001929"
     tRNA            complement(15..90)
                     /locus_tag="POB92_001929"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ala,seq:tgc)
     gene            complement(114..187)
                     /locus_tag="POB92_001930"
     tRNA            complement(114..187)
                     /locus_tag="POB92_001930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Pro,seq:tgg)
     gene            complement(208..284)
                     /locus_tag="POB92_001931"
     tRNA            complement(208..284)
                     /locus_tag="POB92_001931"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Arg,seq:acg)
     gene            complement(290..378)
                     /locus_tag="POB92_001932"
     tRNA            complement(290..378)
                     /locus_tag="POB92_001932"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Leu,seq:taa)
     gene            complement(386..460)
                     /locus_tag="POB92_001933"
     tRNA            complement(386..460)
                     /locus_tag="POB92_001933"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(426..428),aa:Gly,seq:gcc)
     gene            complement(494..566)
                     /locus_tag="POB92_001934"
     tRNA            complement(494..566)
                     /locus_tag="POB92_001934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Lys,seq:ttt)
     gene            complement(573..648)
                     /locus_tag="POB92_001935"
     tRNA            complement(573..648)
                     /locus_tag="POB92_001935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(613..615),aa:Thr,seq:tgt)
     gene            complement(665..740)
                     /locus_tag="POB92_001936"
     tRNA            complement(665..740)
                     /locus_tag="POB92_001936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Val,seq:tac)
     gene            complement(753..867)
                     /gene="rrf"
                     /locus_tag="POB92_001937"
     rRNA            complement(753..867)
                     /gene="rrf"
                     /locus_tag="POB92_001937"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="POB92_001938"
     CDS             complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="POB92_001938"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6579411.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDDEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2965..3057)
                     /locus_tag="POB92_001939"
     CDS             complement(2965..3057)
                     /locus_tag="POB92_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579412.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3451..3927)
                     /gene="folK"
                     /locus_tag="POB92_001940"
     CDS             complement(3451..3927)
                     /gene="folK"
                     /locus_tag="POB92_001940"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDI6579413.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSLKRYK"
     gene            complement(3924..4289)
                     /gene="folB"
                     /locus_tag="POB92_001941"
     CDS             complement(3924..4289)
                     /gene="folB"
                     /locus_tag="POB92_001941"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDI6579414.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4267..5070)
                     /gene="folP"
                     /locus_tag="POB92_001942"
     CDS             complement(4267..5070)
                     /gene="folP"
                     /locus_tag="POB92_001942"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI6579415.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGVDII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(5286..6218)
                     /gene="cysK"
                     /locus_tag="POB92_001943"
     CDS             complement(5286..6218)
                     /gene="cysK"
                     /locus_tag="POB92_001943"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI6579416.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(6397..7278)
                     /gene="hslO"
                     /locus_tag="POB92_001944"
     CDS             complement(6397..7278)
                     /gene="hslO"
                     /locus_tag="POB92_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI6579417.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(7507..9600)
                     /gene="ftsH"
                     /locus_tag="POB92_001945"
     CDS             complement(7507..9600)
                     /gene="ftsH"
                     /locus_tag="POB92_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI6579418.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         2924 a         2065 c         1352 g         3360 t
ORIGIN      
        1 gtgtaaaaaa taaatggtgg agactagcgg gatcgaaccg ctgacctcct gcgtgcaaag
       61 caggcgctct cccagctgag ctaagccccc ataaaatgga atttaagaag taatcgggaa
      121 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
      181 cttcccgtta ttcaattaat ttaaaaatgg cgcgcccgat aggagtcgaa cccataacct
      241 cttgatccgt agtcaaacgc tctatccaat tgagctacgg gcgctaaaat ggtgccgagg
      301 accggaatcg aaccggtacg gtgatctctc accgcaggat tttaagtcct gtgcgtctgc
      361 cagttccgcc accccggcaa aataatggag cagaagacgg gattcgaacc cgcgacccca
      421 accttggcaa ggttgtattc taccgctgaa ctacttctgc attattttgc taaatataaa
      481 aattaaatcg taatgagcca tagaggattc gaacctctga ccctctgatt aaaagtcaga
      541 tgctctacca gctgagctaa tggctcaaaa gatggtgccg gccagaggac ttgaaccccc
      601 aacctactga ttacaagtca gttgctctac caattgagct aggccggcta tatctaagaa
      661 taaatggtgg agaatgacgg gttcgaaccg ccgaccctct gcttgtaagg cagatgctct
      721 cccagctgag ctaattctcc gatttgtcat ttgcctggca acgttctact ctagcggaac
      781 gtaagttcga ctaccatcga cgctaaggag cttaacttct gtgttcggca tgggaacagg
      841 tgtgacctcc ttgctatagt caccagacaa aataacttac ttattcatga tacacgctta
      901 attaataagg tgcaatagtt aattttacac tttgatgtaa actttgtcac atcagctttt
      961 agtgtgtttc gtctgttttt gacgactttt atatattact tcattcaatt gataaagtca
     1021 acacttttaa tcaacttttt tattcttcaa aactgacatt tctcgtgccc tctaattcat
     1081 aacactttta gtattctatc atacacatat gtacaaaacc atacacattc tatacaattt
     1141 taaagtactt ttatagctga ttaagataaa ttatactaat atagcaattt ttgttataag
     1201 atatctcaat gtctatcgtt aacatttctc atccgaacta ctctatatac gtatcgtcat
     1261 tttcatctat cagccaacta ccttgtttaa cagtcaaaaa tagcaccact atcgttaact
     1321 attactatca aaaacattca aacacgaact aattaccata attaagaaag ccctcgcata
     1381 cacatgttgt atacacgaga gctcttacta acaatcaacg tcatttattt ttgtctcata
     1441 taagggaata ataatacgtc tctgattgat ggagagtcag ttaataacat aaccaatctg
     1501 tcaataccga taccaagacc acctgtcgga ggcataccat attctaacgc ttcaatgtaa
     1561 tcttcatcca tttcatgcgc ttcatcatta ccttgcgctt tttcaacaag ttgcgcttca
     1621 aaacgacctt tttgatcaat aggatcattt aattcagtaa atgcatttgc atgctctcta
     1681 cctacaatga acaattcgaa acgatcagta aatctaggat cttcaggatt tttcttcgct
     1741 aaaggtgaaa tttcagtcgg atgaccatag ataaacgttg gctgaataag tgtttcttca
     1801 actttttgct caaagaattc atttaaaata tgaccatatt tcattgtatc tttaatttca
     1861 ataccatgtt ctttagctaa agctttagct tcatcatcac ttttaacttc ataaaaatct
     1921 acaccagtag cttcttttac agcatcaaca atatgcaaac gagtccaagc agattctaaa
     1981 tcaatcgttt ccccattgta ttgtactttt gcagaaccta acacttcatt ggcaatatgt
     2041 ctcaccatag attctgttaa atccataatg tcatgataat ctgcatatgc ttcatataat
     2101 tcaatcattg tgaattcagg gttatgtcta gttgatacac cttcattacg gaatactcta
     2161 ccaatttcat atactttttc aagtccaccg acaattaaac gttttaaatg caactcaata
     2221 gcaatacgca tgtataacgt tgcatctaat gcattatgat gtgttacaaa tggtctagca
     2281 gctgctccac cagcaatttg gtgcatcata ggtgtttcta cttccaagaa acctttatta
     2341 tttaaataat tacgcatttc ttgaatgatt ttactacgat taataaatgt acgagtgcta
     2401 tcttcgttcg taattaaatc taaatatctt tgacgatatc tctgttcaat atcctgtaaa
     2461 ccgtggaatt tatccggtaa tggtcgcaat gatttagtta gtagcgtgaa tttcttcgct
     2521 ttaaccgata attcgccagt atttgttttg aacattacac cttcaacacc aacgatatcg
     2581 cctaaatcag catttttcca taaatcaaat tcgtcatcgc caacttgatc tttacgaacg
     2641 taaatttgaa tttgtccagc taagtcctga acgtgtgcaa atcctgcttt acctttacca
     2701 cgcttagtca ttaatcgtcc agctatagcg acatgactat ccgcttcttt ttctaccaat
     2761 tcttctttag aatactggtc ccactcttct ttcaaatcac tagataaacc tgaacggtca
     2821 aatttagaac caaacgggtc tataccaaga tcatataatt cttgtaattt ttgacgtcga
     2881 accaacattt ggtcattcat ttcttctgac ataactttct ctcctttaac ttgttgtaat
     2941 cctttagtat tccaactggt acactcaaac attttccttt aaagttttat aaaatatcag
     3001 acatttttgc tcaacataca tctctttcga tgtttctaat actttatcat taaccattac
     3061 accttaaagt tatgatgtgg catgttttct tatattcata acatcaattt tatcactttt
     3121 ttaattttac aagagcttgt aaatgtaata aagcaatttc tgttttcgtt tatgaattac
     3181 ttattcattt aaaatacgat tatcattaat acaaccctaa gtaaatgtat aaaaattatc
     3241 ttccacaaac ttcaacaaaa gcccaaataa attacagcaa tttatcaaat attgcttact
     3301 ttgattttat gaaataactt aattctaaca catactaaat catcatatac taattcgaaa
     3361 tcaaatgcat ttagagataa tcaaaatgcg gaaacatctc caataatcaa tcatctattc
     3421 ccaataaata tgaatgttct caacaatgca ttatttatat ctctttaaac tgtcatctac
     3481 aaaaactaaa tctttcactt tcaatttcga acgtggttct acgacatttg ctgctatatc
     3541 atttaatggg attaaaacaa atgcacgttc attcattctc ggatgtggca ccgacagttt
     3601 tggtaaatct atcatttctt ctccatacaa caaaatatcc acatctaaag ttctaggacc
     3661 ccatcgttcc tttctaatac ggtgtaaaca ttcttctgtc ttcaaacaac attccaacag
     3721 ttgtaatact gtgagtgttg tttgaatttc aacacacaaa tttaaaaagt taggttgctc
     3781 agtataccca actggtgctg tttcataaat cggagaaata ttagatacgt taataccatc
     3841 atattcattc aaaatcttta tagcatcgtt taactggctt tctctatcac caatattact
     3901 acctaatcct aagtatgctt gaatcattta ttctccctca ctatttcgat acctactcca
     3961 tcataatgac ccggaatcgg tgggttttct ttagtgattc tcactttcgt ttccattaca
     4021 cgattatatt gtgaatttat acgatttgca atacgttcag ctagatgctc aagtaaatta
     4081 acggccttac cttccataat tgatttaacc tcttcgaaca cttcaccata atgaactgta
     4141 tcaataacat tatcagtacg cccagcttca gctaagtcta ctttcaaagt tacatccact
     4201 ttgaaaattt gccctatttc attttcagct gataaagcac catgatatcc ataaaagcgc
     4261 atacctttaa gaaagattgt gtcttgcatt ttcattctcc tttaaaaaat ctataccttt
     4321 agctaattta gcattcaact cgacattatg aacgcgtact gctctaacgc ctttcataat
     4381 accatatgca gtcgtagctg cagttacttc atctctttca accggtgttg tatcataacc
     4441 catcatctct ttagtgaaac gtttccggct tgtcgctaat aaaactggat attctgttgc
     4501 aacaagttca tccagtcttg ccataacttc ggcttcttca tttctagttt tagcgaaacc
     4561 tatacctgga tctagccaaa ttttatttga aggtatacca gctattttag cttgatgtgc
     4621 ttgtgctaac aaagatgtta acatttcttc gacaaccggt tcatcacgat taccatttcc
     4681 attatgcatt aaaataattt ccgcgtcata tttagctaca atttggaaca tacgatgatc
     4741 atacagaccc gcccattgat cattaatcat atcaacgcct aattttaaac atgcttcagc
     4801 aacctcactt cgaaatgtat cgactgaaat ttttacatca aaaccgacaa tagcttcaac
     4861 aacaggtaat actctgttca tctcttcttc taatgaaacc atttcatgac ctggtctcgt
     4921 tgaaacacct ccaacatcta taatgtcaac accttcatct atcatggctt tcactctatt
     4981 tatagctgat tcaacattat taaattttcc accatctgag aatgaatcag gtgtgacgtt
     5041 taatatgccc ataatttttg ttttagtcat gttaaccacc ctttaattat gtattgttta
     5101 actctaataa aatttactcc tgctatttac tagttattag tatgtacgtt tattatctta
     5161 tcattcactg gatattttta tcagaaaaat gtttaattta acgctcatat ttttaaaaat
     5221 atacatggta gaaaaaaaga gcaagttatc tcaaaaagat aactcactct tttaaatgac
     5281 attaattagt catcgaatga atataaaggt gttgataagt agcgttcacc attactcggc
     5341 aatactgtta ctactgtttt accttttcct aattcttttg ctttttgaat ggcagcataa
     5401 atcgcagcac ctgatgaaat acctgctaaa ataccttcct ctttagcaac tcgacgagac
     5461 atttccatcg ctgtatcatt tcctacttta ataatactgt catagatttc tgtattcaaa
     5521 gtgcctggaa taaatccagc acctaaacct tgtaatttat gtggacctgg ctcaccaccg
     5581 ctcaatactg gagaagcctc aggctctata gcaacaattt cgatgttagg atattctttt
     5641 ttcagaactt tacctacacc agataacgta ccaccagtac caacaccagc taggaacgca
     5701 tcgatagttt tcccttcaaa ttgttgtaat aactcaggac ctgtagttaa ctcatgaact
     5761 tcagggttcg cagggttttc aaattgttgt ggctcgaagt aaccatgttc ttctttcaat
     5821 tctttagctt ttttaattgc acctttcatc gcttctgatc caggcgttaa aactaattcc
     5881 gcaccgtatg cttttaataa attacgacgc tcttggctca ttgtttcggg catagtaaat
     5941 actgctttat atcctttagc agcacataca aatgctaaac cgatacctgt attaccactt
     6001 gttggttcta caattgtatc gccaggttta attttgcctt ctcgctctgc tttttcaatc
     6061 attgctaaag caattctatc ctttacagaa ccacctggat tttgatattc caattttaca
     6121 taaacatctg ctgcattgtc atctactaca tttctcaatt tgactaccgg tgtaccgcca
     6181 ataatttgag taatattatc tactggtttt tgtgccatta agaacactcc tttttagtta
     6241 gttgttgaaa tcttagttta tttatcggat atagaattat tttatcaaat aaattctaga
     6301 aagcaaacaa aatcactaag gattatacaa ctatttagtt ctacatttca ctaaaccgct
     6361 aaaaataaac attttagcgt attgatttaa attaaattac gctaaacttt ctagcaacac
     6421 gtttaattct tcttcagtat atttatattt atttccacag aaatgacata ctgcttcagc
     6481 accatgatct tctttaatca tattttgaat ctcagcctcg cccaatcctt taatagcatt
     6541 taaaaatttc tcatgactac aattacattc aaattgaaca ggcattttct ctaaaatttg
     6601 cacatggtct tcacctaaga tttcgtttag taatccttct ggcgttaatc cttgttcaat
     6661 taatttagaa actggtgtca tttcactaat tgctttttct aattttgaaa ttgtttcatc
     6721 tttggcacct ggcataactt gaatgataaa tcctcctgct gctttaatcg tattatcagg
     6781 atttaccaat acaccaagac ctaccgatga aggtgtttgt tcacttgtag cataataata
     6841 agtaaaatct tcaccaagtt ctcctgaaac aattggactt gctccagaga aatagtcttt
     6901 cattccaacg tctttaacaa ccataataga tccatttgtc cctactgctc gtcttacatc
     6961 aagtttacct tgctcattta atggaaaatg agtttgtgga tggtctacat aagcacgcac
     7021 ctcgccttta gcatttgcat cggcaataat tcgtccaata ggtccttggc catctacagt
     7081 gacagttaat ttttgatcac ctttcaacat tgcgcccatc atagctgttg ctgtcattgt
     7141 tcttcccatt gcagcagatg ctgtcggcca tgtataatgt ctcgtttgtg cttcttgaac
     7201 agtttcagtt gtcaaagcag cataagccct aatctctcca tcaaatgcta atgctttaac
     7261 aatataatcg tgtgtcatta tttcaatctc ctctattact ctatatttaa aaaattactt
     7321 tacttcataa aatgcaacaa ttgtacttat tctacaccca tcattctaaa taatgaagta
     7381 acttgtttta caattatttt ctgctataac aattcaacga cttaaaatct aatacgtatt
     7441 ttcaaaaacg ataaaagtac ctctttctat aactttatca tagaaagagg tactgaatat
     7501 aatcgattat ttattgtctg ggtgatttgg atcgtaaggt ttttcgatat ttggggcttg
     7561 tcgatgtgct ggttcatctt tttcatcaga tttatcagct tcttttttat cctcagcaat
     7621 atctttttct tctttacgat cttcactttc gtcacgttgt ccatcttcta attgctcttt
     7681 acgaatctct tcataagatt taccgaattt accatcatta aattcagaat cttcatcttt
     7741 aacaacttta gctgcatcat aatcaatttc aggtaattta ccttcgtaga ataatgattg
     7801 aatttgttca gcaactaatg tttcttctgt taataatgtt tcagcaatta aaattaattg
     7861 ttctttgtgc tctaataaaa tttgtttaca acgttcgtat tgttctttaa cgattcgttg
     7921 aacttcttta tcaatttcat atgcgatttg gcttgaataa ttaggctcac cttgcatatc
     7981 tttacctaag aatacttgac cattgctatg accgaactgt aatggtccta attttttact
     8041 cataccatat tgcgtaacca ttgagcgtgc gatttgtgtt gcacgttcga agtcatttga
     8101 agcacctgtt gatacttcgt taaagttaat atcttctgat acacgtccac caagtaaacc
     8161 acagatttta tctaataact cttgttcagt cattaagaaa cgatcttgtt taggtagcat
     8221 cattgcataa ccacctgctt gtccacgtgg aacaatagta actttatgca ctacttctgc
     8281 ctcatcaagt accataccga taattgtatg accagcttcg tgatgagcaa caatattacg
     8341 ttctttctta gaaataactc gagatttctt agcaggtccg gctataactc tatccgttgc
     8401 ctcttcgata tctctcatgt caattttctt tttaccttca cgtacagcaa ttaaagatgc
     8461 ttcatttaat aagttctcta aatcagcacc tgagaaacca ggtgtacgtt gtgaaattgc
     8521 ttttaaatca accgtttcat caagtggttt gtttttagca tgaacatgaa gaattgcttc
     8581 acggcctttc acatctggac gaccaacttg aatttgtcta tcaaaacgac ctggacgtaa
     8641 taaggctggg tcaaggatat caggacggtt tgtagcagct atcataatga taccttcatt
     8701 ttcaccgaaa ccatccattt caactaataa ttggtttagg gtttgttcac gttcatcatg
     8761 accgccacca acacctgcac cacgttgacg accaacagca tcaatctcat cgataaagat
     8821 gatacaaggc gcgtttttct tagcattatc gaataagtca cgaacacggc tcgcaccaac
     8881 accaacaaac atctctacaa agtctgaacc actaatagag aagaatggtg cgccagcttc
     8941 acctgcaacc gctctagcaa gtaatgtttt accagtacct ggaggtccaa caagtaagac
     9001 acctttagga atcctagatc ccatttcttt gaatttttta ttatctttca agaaatcaac
     9061 aatttcaatt aattcttgtt tttcttcatc tgcccctgct acatcagaga aacgaacacg
     9121 acgtttatta ttatcgtaca tttttgcttt agatttacca aagttcatca tacgaccgcc
     9181 actaccgcca ccttgtgctt ggcttaggaa gaaaataaat aataacgcta tgactacaac
     9241 tggaattaat gttgaaagta tactcacaaa gacactttgt ttttcttctt ctttaatcgt
     9301 taattttaca ccgttttgct ttttagcagc atcagtaatt ttttgtaatt ctttttcgtt
     9361 gttatataaa atagttgatg aatagtcttc atcatttttc gttttaccac ttaccatata
     9421 gacattttgt tgtggttgga tttctaaagt tttaaggtca cctttttcca acttctcagt
     9481 aaattgatta tatgtaagct gtttcggcat atttccatta ccgtttaaat atgaaaatag
     9541 accaaaaata ataacgccta ttattacgat aactagcaca ttgcgaaaag ctttctgcat
     9601 gcgtcatttc ctcctacttc ccataataaa actaacaaaa attgtaacat atgacatgtt
     9661 ttataagcta gtaattgagc ttaacatcaa tgtcattcac a
//
LOCUS       DAMZLE010000092         9690 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMZLE010000092 DAMZLE010000000
VERSION     DAMZLE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.093"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            152..295
                     /locus_tag="POB92_001946"
     CDS             152..295
                     /locus_tag="POB92_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDI6579419.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            360..3353
                     /locus_tag="POB92_001947"
     CDS             360..3353
                     /locus_tag="POB92_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HDI6579420.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTE
                     IGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLWLLSR
                     NNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVMLKQK
                     FLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAELYLGRFYNQYNELIVIS
                     PLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDDNVEMISINNIPKYPRN
                     HNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLFESNN
                     MYNFLKECNLYRLLREISMESVKCIESMNDVSIDSFSYSPRIRYKNVILKPAYWKINE
                     MVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLSLANHRYLLMKEYKKHK
                     RVRLVESFLPQSKNDHVYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIHI
                     DKPSQDTFIIDNLYPFVKHLKDKGDIEKYFLMRYIKQGDILKLRLFRNDENYAEIYSI
                     LKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFK
                     FDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANN
                     FEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKD
                     QETFVLSIVKEIVKTQKHWCGDKND"
     gene            3346..4590
                     /locus_tag="POB92_001948"
     CDS             3346..4590
                     /locus_tag="POB92_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HDI6579421.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            4606..5124
                     /locus_tag="POB92_001949"
     CDS             4606..5124
                     /locus_tag="POB92_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HDI6579422.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            5134..6507
                     /locus_tag="POB92_001950"
     CDS             5134..6507
                     /locus_tag="POB92_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDI6579423.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            6530..7222
                     /locus_tag="POB92_001951"
     CDS             6530..7222
                     /locus_tag="POB92_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HDI6579424.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            7219..7980
                     /locus_tag="POB92_001952"
     CDS             7219..7980
                     /locus_tag="POB92_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDI6579425.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            7977..8675
                     /locus_tag="POB92_001953"
     CDS             7977..8675
                     /locus_tag="POB92_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HDI6579426.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(9149..>9690)
                     /locus_tag="POB92_001954"
     CDS             complement(9149..>9690)
                     /locus_tag="POB92_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDI6579427.1"
                     /translation="LVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEEKMTDGQLWK
                     KVRDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSIDFKGLKQID
                     LTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSPVSGKVGEGV
                     TSGGENTLTKRFINKITID"
BASE COUNT         3574 a         1217 c         1614 g         3285 t
ORIGIN      
        1 ctaattatca aaatagtatt actaaattaa taagtgttta caaaaaattc acaaaatata
       61 attatataat ttattgattt gttgtgtgaa aagggataat atgacaattg aagtaggcct
      121 taacttcaac taataatagg aggttttatc tatggaaaaa gttcttgatt tagacgtgca
      181 agttaaagca aacaataact caaatgattc agcaggtgac gaacgtatta caagtcatag
      241 tttatgtact cctggttgtg ctaagactgg tagttttaat agcttctgct gttaagatta
      301 cagcaaatag agcctgaaag ggctctattt gtctattcaa atatgtgagg tgcttacaaa
      361 tgaacactat ttacgaaccg tcaagtattt gtatgattag aacaccttta ttatcagttg
      421 aatttttcaa tttattttta aatactgaac aaattaaata tagcgactta caattaaatg
      481 ctcagatgaa agaatcaatc ctaacaacaa catttaactt atattgtact ttacaagaaa
      541 taaattttga tggcgataac aagaaggtta gagatgctaa agaaagttta ttaaaatatt
      601 taattagaat gtcaacacgt ccaactccat ttggattatt aagtggtatt aatttggggc
      661 attttgtaaa tgaaccaaca cgtcttaaag ttggaaatag tattcaaaaa tatgtgaaag
      721 tcgatggcga atggttgtat aaactggtta gttatattga gagtattgat gaatattatc
      781 aaaatttgaa agttatttgg aatagtaaag cacatattat caatgatcga atatatttaa
      841 atgaacaaag tgctatttat ttaaacaaca ataaagatac atctttttcc attaaaaata
      901 gcgaattact cgtattcatc aaaaccactg tgacgaataa taatattact ttttcaaatt
      961 tagctgaaaa gataaatcaa gaatttgaaa ttaatgatat tactaaagtg aaagtatata
     1021 tccataatct tgtctcaaag gaaattatat attcaacaat cagaccaccg ttatcatata
     1081 gcgataattt aaattatatt ttaaataaac tttctttaca taatgatgat tttgtgaaaa
     1141 aaattagaga aatccagaaa ttgatattag cttatgaaaa aactgaaatt ggatttggcg
     1201 aggaattata taaagacatt attcatcata tgaaggcact attcaaatgt aaaaactatc
     1261 ttcaaattga taccaaaatt gacatgatta acaattattt acatcaagat atagcaacaa
     1321 atatatctga agcagcgtac ttattgtggc ttttatcaag aaataatatt ggattcacag
     1381 atttgaaagt tcttcacaac cgttttattg aaaagtatgg atttgaacaa ctcgtgaata
     1441 ttaaagattt gatttcagat ataacaggtt tcgggacaac aatttttcaa gaagaagaaa
     1501 ccgatggaaa taatattgta atgcttaagc aaaaattttt acatgccttg agaaataacg
     1561 atgagatagt cataaatgaa aaagatgtag agtcattgat taacgataat gaaatcaatc
     1621 attatcatgc gccaatgagt gcagatgttt atgctgaact ttatttagga cgtttttaca
     1681 atcagtacaa tgagctcatt gttattagtc ctttaacggc gtcttttaat gctggcgcta
     1741 catttgggcg atttcatcat ttaattgata ctgaaacttt agcaaaatta gaacatgaaa
     1801 aaggacatta ttatcagaag atgatatgtg atgacaatgt agaaatgatt tctataaata
     1861 acataccgaa atatccgaga aatcataatg tattaactaa tcatgactca tacgaatatt
     1921 cattgaattt aggaagtagt aatagttatt caaagtatga gcttacctta gatgatattt
     1981 atgttggtgc tacctttaac aaattatatt tatattctag ccaactaaat aaaagggtac
     2041 tatttgaatc aaacaatatg tataactttt taaaagaatg taatttatat cgattgttac
     2101 gagaaatttc aatggaatct gtgaaatgca ttgaatctat gaatgatgtg agtattgatt
     2161 catttagtta ttcaccacga atcagatata aaaatgtcat tcttaaacca gcatattgga
     2221 aaattaacga gatggtatta cctttaccta aaaatgaaga atgggatcaa caatttttga
     2281 aatatcaaga acaattcaat atacctaata tcgtgaattt agtttatgga gataacaagc
     2341 ttctactaaa tttatcatta gctaatcata gatatttatt gatgaaagaa tataagaagc
     2401 ataaacgtgt acgtttagta gaatcatttt tacctcaatc aaagaatgat catgtgtatg
     2461 aaattgttac accaatttat aaaaaatcat cttatcgagg acccgaaatc gaaattccaa
     2521 aatataaaaa tacagatatt gaatatgaca aagactggtt tgcattacat attcatatcg
     2581 ataagccatc gcaagataca tttatcatcg acaatttgta tccatttgta aaacatctta
     2641 aagataaggg agatatagaa aaatattttt taatgcgtta tatcaaacaa ggtgacattt
     2701 taaagttgag gttattcagg aatgacgaaa attacgcaga aatatacagc atcttgaaga
     2761 attggttacc acatgttcga caaacgacag aagtttcaga ttatgaattt gtatcatacg
     2821 agccagaatt ttttagatat ggtggaaaga acacgattaa tgaaatagaa gccttctttg
     2881 aatatgacac gaatttagct gtcaatatta ttgaaaatga ttttaagttt gatcgaccat
     2941 atattgttgc tatttcaata atgtatttat ttgagatgtt ttcaatttct aatgaagagc
     3001 gtatggaaat tgttaataac tatgtaccta catcatttaa aagtaaagat attagacctt
     3061 ttaaaaatga acttgtaact atatgtaatc ctgctaataa ttttgaatat atggctaaac
     3121 actatagtgg tatttatcga attttaaaag atggcaacca aattttgagc aaattaaatg
     3181 aagggcttaa aaaaacttta actactaaaa ggtcaaggat tattggcagc ttaattcata
     3241 tgcgctgcaa tcgaatattt ggcattgata aggatcaaga aacatttgtt ttatctattg
     3301 taaaagaaat tgttaaaact caaaagcatt ggtgtggtga caaaaatgat tgatatgaat
     3361 ctaatattga agaagaagtt tcaagagatt tcagaagtag atgatttcat ttcaaaagca
     3421 tctactgaaa ccgattattt tgaaccttca actttatcac atggtatacc aggtatcatt
     3481 ttgtttttag atgcttatca aaaagttttc gatattaata ctgagcaaat tgtgcataag
     3541 tacatcatga aattagcacc atatttacag agtggtcaat ataacaattc tttgtttggt
     3601 ggactttcag gtattgcatt ttcaatggat ttagcatcgc aaaatggcaa aaattatcaa
     3661 aatattttaa ataatattga tggtcacatt gtaaatgaaa tagaaaataa atttgatcaa
     3721 atattacaag aaccattaaa tccattaaat tatgatactg tcagtggatt agctgggata
     3781 gggagatatt tgctaaatag agtagatgag aatgaattta atgttaaagc attaaaaagc
     3841 atattagtat actttaaaga tattcaatat tctaaaaata gctgggtagt cccacaagaa
     3901 agtcaatttt tagagtctga taaaaattat tttactgaag gtaatatcaa tcttggcctt
     3961 gcacatggag tgctaggacc gatgtcttta tttgcattat gcgtgattaa aggaattacg
     4021 attgaaaatc atcagcacat attaaaagac atgtacaaat ttatcatgga cgaaaaattt
     4081 tgtaaccacg aaagatggtt gcagcgttac gatttaattt ctgaacgtaa tcatttcaat
     4141 tttattcgga atggttggtg ttatggcaat acgggtgtaa tgacgacgtt gtttttaatc
     4201 ggccaagcat tacaagatga tgaaataatt aaaatgtcta aaaaagtgat gctacaagta
     4261 gtaaatgata aagatgaaaa tttaataagt ccaactattt gtcatggatt gtcatcacaa
     4321 atattaatgt taacaattat gaatttgaat tttgaattaa atgaagtgtc tgattatatc
     4381 actgtattaa taaataaact gatttctcat tataaggaag attatctggt gaattttata
     4441 gacattaatg aaaataagca agatgtattt aaaagtagga aagttggcct tttagaaggt
     4501 gaattagggg tctatctaac attgatgaca ctgaaaaata caaaaattct caatgaaaaa
     4561 aattggacca atgcgttttt aattagctga atggaggcgt atatcatggg agaaaatgta
     4621 ttgatttgtt tgtgtggctc agttaatagt atcaatatta gccactatat tattgaatta
     4681 aaatcaaaat ttgatgaagt aaatgtaatc gcgtcaacaa acggtagaaa gtttattaat
     4741 ggtgaaatat taaagcaatt ttgcgataac tattatgacg aatttgaaga ccctttttta
     4801 aatcatgtag atatagcaaa taaacatgac aagattatta ttttacctgc gacttctaat
     4861 acgattaata aaattgcaaa tggtatatgt gacaatttat tattaactat ttgtcataca
     4921 gcttttgaaa aactttctat atttccaaat atgaatttac gaatgtggga aaatccagtt
     4981 actcaaaata atattcgatt attaaaagat tatggtgtat caatatatcc agcaaatatt
     5041 tcagaaagtt atgaattagc gtcaaaaaca tttaaaaaga atgttgtcgc acctgaacca
     5101 tataaagttc tggaattcat ttgagattag aatatgaaaa tcataaagcg cgctattatt
     5161 agtttaataa tattgagtct tttaatatct attacaatga gtaatgcatc agcgtcagaa
     5221 gaactatatt acagtgttga atacaaaaat acagcaacat ttaacaagtt agttaaaaag
     5281 aagtccttaa acgttgtcta taatataccg gaattacatg tggcacagat taaaatgacg
     5341 aaaatgcatg ctaatgcttt agcaaactat aaaaatgata ttaaatatat caatgccaca
     5401 tgttcaactt gtattactag cgagaaaaca atagacagaa catcaaatga gtcattattt
     5461 tcaagacaat gggatatgaa taaaataacc aataatggtg catcgtatga tgatttgcca
     5521 aaacatgcta acaccaaaat agcaatcata gatacaggtg tgatgaaaaa ccatgacgat
     5581 ttgaaaaata atttctcgac tgattctaaa aatttagtac ctttaaacgg ttttagaggt
     5641 actgaaccgg aggaaacagg tgatgttcac gatgtcaatg ataggaaagg acatggcacg
     5701 atggtgtcgg gtcaaacgag tgctaatggt aagttaatag gtgttgcacc gaataacaaa
     5761 tttacaatgt atcgcgtgtt tggtagtaaa aaaacagaac tgctttgggt atcaaaagcg
     5821 attgttcaag ctgcaaatga tggaaatcaa gtcattaata ttagtgttgg tagttatatt
     5881 attttggaca aaaatgacca tcaaacattt agaaaagatg aaaaagtaga atacgatgcg
     5941 ttacagaaag caatcaatta cgccaagaag aaaaaatcta tcgttgttgc tgcagctggt
     6001 aatgatggta ttgatgtcaa tgacaaacag aaactaaaat tacagcgtga atatcaaggt
     6061 aatggcgaag tgaaagatgt tcctgcatct atggacaatg tcgttacagt aggatctaca
     6121 gatcaaaaga gtaatctatc tgagttttcc aattttggta tgaattatac agatattgct
     6181 gcgcccggag gatcatttgc ttatttaaat caattcggtg tggataaatg gatgaatgaa
     6241 gggtatatgc ataaggagaa cattttaact actgccaata acggaagata tatttatcaa
     6301 gctggaactt cattagccac acctaaagtt tcgggagcac tagctttaat cattgataaa
     6361 tatcatcttg aaaaacatcc agataaagcg attgaattgt tatatcagca tgggacatct
     6421 aagaataata aaccatttag tagatatggg catggtgagc ttgatgtgta taaagcatta
     6481 aatgtagcaa atcaaaaagc aagttaataa atcaaaggag tttttgatta tggcaaaatt
     6541 agttactgaa aacatttcga aacggtttaa aaatcaagat gtattaaagc atattaatat
     6601 cactttagaa aataacgaag tttatggatt acttggtatt aatggagccg gtaaaacgac
     6661 acttatgaaa attatatgtg gcatacttca acaagattca ggggaaatta aattagataa
     6721 tagaccaatg acacgaaatg atttgcacaa agttggttcg cttattgaaa cacctgcgac
     6781 atataatcat ttaagtgcac aagataattt gaaaattgtg tgtttaaatg aaagcgttga
     6841 tttcagcgaa attaatagtg ttttaagctt agtcaattta aatgtcgata aaaagaaaaa
     6901 ggttaaggac ttttctttag gtatgaaaca aagacttgga attgcaatgg cattaattaa
     6961 aaagccagaa attttagtat tagacgaacc atctaatggt ttagacccat atggaatcca
     7021 agaacttaga gaacttctaa aattattaac agaacaaggt actagtatta ttatttcaag
     7081 tcacatttta tctgaaatcc aagttttagc agatcatatc ggtattattc atgagggtga
     7141 gctaaaatat cagcaaagaa ataacaaaga tgaaaactta gaagagatat tcttcaaaat
     7201 aacgaaaggt gattacaaat gatacattta aagatagaag gtatcaaatt taaaaattct
     7261 ttcagtatgt atgttttatt aataagtccg ctggtatttc tttgttttgc tattttcaca
     7321 gtcttattcg ccaaaagtaa tacgggaaca gcgaatagtg tgtcaccata tataacttta
     7381 ctatttaata tttggccaat tgctttcatc ccgattgtat tatgtatggc ttgtaattcg
     7441 ttatttaaaa ttgaaatgag aaataaatca tttaattatt acttaagtaa taattggtcg
     7501 attacaaaag aaataagagc aaagattttc attttatcaa tagcattttt ggtacattgc
     7561 tttttagtat ttattattgc ttatataggt gacctaatta ttaatccaca tccggttaat
     7621 gctatgttgt tattggtgac aatattgttg atgtatgtag tatctctacc attgataccg
     7681 ctcaactttt tattaactcg atactttggt gtgttcgtat caatattaat aaacttagta
     7741 ttatcagtca tttgtgtctt gtttttaaca ttgaagagtt tattttgggt gttgccgtgg
     7801 gggataatgc agagaatccc gcttattacg cttggtatac tacctaatgg tttagttgta
     7861 aaccataatt caaaatactt taatgatctc aatgccttat atatttcgat tattgttagc
     7921 atcattattt tcgcgatagt aacattttta aataataaga aaagttggcg attaaaatga
     7981 taattaacga attaaagtca tgtaagttga aattttctaa gcaagcgctc acatttgtac
     8041 ccattattgt aaccatattg tttatattat ttataaattg gtatttaaac gtaaatttat
     8101 ggaatggtcg acaaataagt ttgtttacag cgagttttaa tgcaattaca tcgctattaa
     8161 tttctataaa cgtctatcaa gttatcaatt ttgaagaaaa tattggtcac tttaatcata
     8221 ttttaggaaa agctaatagg ctaaattggt taaatgcatc aatgattttt acttatacta
     8281 ttacagccat atgtattcta ttagcatcaa ttaatttatt gtggcattca catgatatga
     8341 aaataacact tatgtttata ggcgtatcat tgtttttcaa tgtaattata ttactgctac
     8401 tttttatttt tagtattttc attaaagatg taatggctat tgttgtcgga gttttaatgt
     8461 ttatttttaa cgtttatttt ggattagaag tgcttggaga tcattcgtgg ttctatttac
     8521 caatcacata tgctacacgt tacgtttata tgtttatcga agggagtata ccagttacat
     8581 taacattggc aatctatatt attatcacgt tgctattagc agttttactc tttaaaggat
     8641 ttaataaatg gagcggtaga acaattaaag attaaagatt ggggctctaa cggtaaaaga
     8701 aatttagttg cgttgaaaag ggaggaaaga gaacagttaa atatgaatac aaaataattt
     8761 tgttttcgga cagcaggggt attagacgcg attgacaatg tctgtttaat taaacgtaat
     8821 gtttatttaa gcgatgaata ttaggtgaaa agtttttgaa tttgaatgta attgaggttt
     8881 attgattaga cattttattg aattgcgtgt tattatataa atgtaaaaat aagacgacat
     8941 gcgcgaacat gtcgtctaag caagccaaac gctggcttct aaaatatttt tttaaccatt
     9001 ccaacttgcc aaagttaatg cggaatggtt tttttatttt ccgctaattg aaataaaaat
     9061 gacgttttaa tatattatgg gctaggtggt ttgtaagaaa gggttagtta ttaatgtttt
     9121 atgaattaag gaaatttgag tttaaggttt aatcaattgt gattttgttg atgaagcgtt
     9181 tagttagagt attttcgcca ccactagtta ctccttctcc cactttaccc gagactggag
     9241 aagagctatc tgaagaataa atagatactt tttggccatt ttgtagtaaa ccaagacctt
     9301 ttaacttctc ggttagagaa ttccatgtat ttttagcatc tagctttttg ttaaagtcat
     9361 cgtagacatt ttccttagtt aaatcaattt gttttaatcc tttaaagtct atagattgtg
     9421 ttagatggcc tccatcatca tttccaggag cagaaacgct gctagaatat ccattgctta
     9481 ataaataagt aacgttgatt gtttcgtact cgttacttaa aataatatca gaatcatgta
     9541 atgaatctct aacttttttc cataattgac catctgtcat tttttcttct gctttagccg
     9601 ttttaacaac tttatttgta tctaatccta agtatgaaga atgtaagcct gttcctaatg
     9661 ttgttaatac taaagcactt gctactaatg
//
LOCUS       DAMZLE010000093         9565 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMZLE010000093 DAMZLE010000000
VERSION     DAMZLE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.094"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            134..598
                     /locus_tag="POB92_001955"
     CDS             134..598
                     /locus_tag="POB92_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6579428.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            777..1901
                     /locus_tag="POB92_001956"
     CDS             777..1901
                     /locus_tag="POB92_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDI6579429.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLFGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            1970..2314
                     /locus_tag="POB92_001957"
     CDS             1970..2314
                     /locus_tag="POB92_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDI6579430.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            2807..2905
                     /locus_tag="POB92_001958"
     CDS             2807..2905
                     /locus_tag="POB92_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579431.1"
                     /translation="MSVGSLAVLTITEIAFPTEIFEKNQRTLQIKF"
     gene            3196..4392
                     /locus_tag="POB92_001959"
     CDS             3196..4392
                     /locus_tag="POB92_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI6579432.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKVQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4382..7318
                     /locus_tag="POB92_001960"
     CDS             4382..7318
                     /locus_tag="POB92_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6579433.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            7315..8256
                     /gene="yhaM"
                     /locus_tag="POB92_001961"
     CDS             7315..8256
                     /gene="yhaM"
                     /locus_tag="POB92_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDI6579434.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRVAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8377..9339)
                     /locus_tag="POB92_001962"
     CDS             complement(8377..9339)
                     /locus_tag="POB92_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDI6579435.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3553 a         1359 c         1629 g         3024 t
ORIGIN      
        1 atacaaatgt gaaattgact cttttcattt tattaaaaat atattaagat gtaatctgtg
       61 tttcaagaaa tgtgtattat tgcaatttct tgtgtccgga taaaattccc caaacagaaa
      121 ggtagataac agaatggata gacagagttt tacagattta attcaaacaa aatttaaaat
      181 ggttcgtata gaggctggtt atacgcaaga tactatggcg caaacaattg gactttctaa
      241 aaagacttta gtacaaatcg aaaaagagag agtattacca aactggacaa cttgtatttc
      301 aatttgtgca ttattcagag actctgaagt attaaacagt acatttggct gtgatccatt
      361 agaaatcgtt caaacaattt caagaaatca ctgtgcatat ccaaaccatg caccaacaag
      421 tgatatttat tggaataaca ttgagactcg taatgggtac attttacaaa gtaacaaagt
      481 aagcaatatt tatcgtgtat taaatccaga taaccaacca attttcggta cttctaaaat
      541 gagagaagct gaaacatact ttaacagaaa tgctaaagaa gaattagtac atatctaggc
      601 ctatatactt ttaataaata ataaaagcga tgtaacacac ctgtttcgag aggtgaggca
      661 tcgctttttg tatgtaaact aataataaat tgagcagaaa gtgaaatgat taatgaatta
      721 aaaaataaaa atagtggtat aatgaataac tatgttgtca caaaggagtc aaaattatga
      781 atgcactatt tattatcata tttatgattg tagtaggggc gataattggg ggcattacta
      841 atgtaattgc aattagaatg ctgtttcacc cctttaaacc atactatata tttaaattta
      901 gagtcccatt tacaccaggt ttaataccga aaagacgcga agaaattgca actaaaattg
      961 gccaagtgat tgaagagcat ttgcttacag aaactttaat taatgaaaaa ttaaaaagcg
     1021 agcaatcaca gcaagcaata gaatctatga ttcaacagca gttacaaaag ttgacgaaag
     1081 atcaattgtc aataaaacaa attacttctc aaatcgatat tgatttagaa caagtattac
     1141 aaactaatgg aaatcaatat attgaatcac aattgaataa ttattataca aagcatcaaa
     1201 accaaacaat agcatcttta ttgccaaatc aacttgtaac atttttagat cagcatgtag
     1261 ataacgcaac agacttatta tgtgatcgtg caaggaatta tttatcatct gcaaaaggca
     1321 cacaagatat taatgatatg ttggatactt ttttcaatga gaaaggtaag ttatttggta
     1381 tgttgcaaat gtttatgaca aaagagagca ttgcagatcg cattcaacaa gaacttatac
     1441 gtttaacatc tcatcctaaa gcaagaacaa ttgtgacatc gttaattacc aatgaatatc
     1501 aaacttttaa agataaacca ttgaatgaac tgttagatgc atcgcagttt aatgaaatcg
     1561 ctgaaaatct atcagtgtat gtaacaacat atgcaagcaa acaagcgaat aagccagtgg
     1621 ttacgttaat gcctcaattc gttgattatt tagaaggtca actctctagc aaattagcaa
     1681 atttaatcat tgaaaaatta tcgattcact tatctacaat tatgaaaaaa gtggatttac
     1741 gaggattaat tgaagaacaa attaatacgt ttgacttaga ttatattgaa aaattaatta
     1801 tagaaattgc taacaaagaa ctaaagttga ttatgtcatt aggtttcatt ttaggtggta
     1861 ttattggatt tttccaaggt ttggttgcaa tctttgtcta aaaaagcgag atgtggtaaa
     1921 gtaatatcga acgttttaat aaacatcaca acaaaggagt gctataacca tggcagtaaa
     1981 tttatatgat tatgcaaatc aattagaaca agctttaaga gaaagcgaag aatacaaagc
     2041 aatcaaagaa gcattcgcta atgtaaaagc taacgaagaa tctaaaaagt tattcgacga
     2101 gttccgtgaa actcaaatta acttccaaca aaaacaaatg caaggtgaag aaattgctga
     2161 agaagattta caaaaagcgc aagaacaagc gcaagcaatt gaaaaagatg aaaacatctc
     2221 tgcattaatg aatgctgaac aaaaaatgag tcaagtattc caagaaatca accaaattat
     2281 cgttaaacca ttagacgaaa tttacgctga ctaatattaa aataatattt ttcaatcctt
     2341 taaatagagg aatagactgc cgacagagtc cgagactttg tgggtagttt ttttagtttt
     2401 gataacggaa gttagaggct ctctgtcaaa ttgggcagat gcgttcagta tcggaattta
     2461 ggctactaaa catgtcctag ctttagctca gaatggacga cggaagctgg gcaatcaaac
     2521 atggctagct ctatgccaaa acgggcagat gagcttgata acggaagtta ggctagtaaa
     2581 cgtgtcctaa cttccttttt gcattttgaa gtgtaaagtt ttcaacataa tactattagt
     2641 tcggtcatgt atcggactga tggaaaagcg tttcactttt aatggctcat taagaacggc
     2701 ctgaaaatgt ttggcgtatt tagtgcaatg atagttttga catttagttt ctaattggtc
     2761 attactgccg agcaaatcta gtagagtaat catgtaaatc tttaatgtgt cagttggttc
     2821 actagcggtg ttaacaatta cggaaattgc atttccgact gaaatttttg aaaaaaatca
     2881 acgcacgcta caaataaaat tttaaactgt tataaatgtg tctcaatttc atatgttcat
     2941 cgacgatatg aagcgtatta tggtaaaatg aagaaataat aaacttgtta ataaataaaa
     3001 catcacgatt tgactaaagc actttattat tgtgtagata ataatttttt aacgaaataa
     3061 aaatggcgac tggttttaat aaatcagcta atgaatcact acacctataa gtatgaatat
     3121 agtgattaga atgctttgta tagttggatt ttgcaaaatt gatgttagaa cctttaatta
     3181 aggagatatg gatgtatggt taaatttatt cattgttcgg atttacattt agatagtcct
     3241 ttcaaatcta agagtcatat aagccctaaa atttttgagg atgttcaaaa aagtgcttat
     3301 gaaagtttta aaaacattgt agatattgca ttacaacaag atgttgattt tgtaattatc
     3361 gcgggtgatt tatttgatag tgaaaacaga acattacgtg ctgaaatttt cttaaaacag
     3421 caatttgaac gtttacaaaa tgaacaaatc tttgtttatg tttgtcatgg taatcacgac
     3481 ccattatcat caaagatttc atcaaactgg ccagataatg tttctgtatt ttcaaataaa
     3541 gttgagacgt atgaagcaat tactaaatct ggtgaaacaa tttatattca cggatttagt
     3601 tatgaaaata gagcaagtta tgagaacaag attgatgaat atccatcaag tcaaggccaa
     3661 aaaggcatac atattggtgt cttgcatggt acgtatagta aatcttcagt taacgaaaga
     3721 tataccgagt tcattttaga agatttaaac agtaaattgt atcattattg ggctttaggt
     3781 catatacatg aacgtcaaca attaagtgat atgcctgtaa ttaactattc aggtaatatt
     3841 caaggtagac attttaatga gcaaggtgaa aaaggttgct tattaatcga gggtgaccac
     3901 ttaaaattaa agactaaatt ttatcctaca cagtatatta gatttgaaga agcaactatt
     3961 gaaacggata agacatctaa gcaaggttta tacgaggtca ttcaaaactt taaagaacaa
     4021 gtgagagaag aaggaaaagc cttttatcgt ttaacgcttg ttattaatag tgagacatta
     4081 atttcacctc aagatttatt acaagttgaa gaaatgatta cagattatga agaaaacgaa
     4141 aatcaatttg tatatattga tgagttaaaa gtacaatatg cacaaaatga tgagtcacct
     4201 ttagttaatg aattttcagc ggaattatta gtcgatcaaa ctgtttttga taaagcgatg
     4261 tcagatttat atttaaatcc aagggcatct aagttcctag acgattatgg aacattcgac
     4321 catacagcat tagttaatcg tgctgaagaa atattaaaag ctgaaatgag aggtgaacaa
     4381 aatgataatt aaatcacttg aaatttatgg ttacggtcaa tttgttcaac gtaaaattga
     4441 atttaataaa aacttcactg aaatttttgg tgaaaatgaa gcgggtaaat cgacgattca
     4501 agcattcatc cattcgatat tatttggttt tccaactaaa aagtctaaag aaccaaggct
     4561 agaaccacgt ctaggtaacc aatacggtgg taaattagta cttattcttg atgatggctt
     4621 agagattgaa gttgaacgaa ttaaaggcag tgctcaaggt gatgtgaaag tatatttacc
     4681 taatggtgct gtgcgtgatg atgcttggtt acaaaagaaa cttaattata tttctaaaaa
     4741 gacatatcaa ggtatctttt catttgatgt actagggctt caagacattc atagaaatct
     4801 aaatgaaaaa caattgcaag attatttatt acaagcaggg gctttaggat caactgaatt
     4861 cacgtcaatg cgcgaagtga ttaatcgtaa aaaagatgaa ttatataaaa aatcaggtaa
     4921 aaatccgatc attaatcaac aaattgagca attaaaacaa ctagaaagtc aaattcgtga
     4981 agaagaagct aagctagaaa catatcatcg cttagtagat gatcgagata aatcatcacg
     5041 tcgattagag aatttaaagc ataatttaaa tcaattatca aaaatgcatg aagaaaaaca
     5101 aaaagaggtt gctttacatg atcattcaca agaatggaag tctctagaac aacagttaaa
     5161 tattgagcca atcacattcc cagaaaaagg tgtggatcgt tacgaaaaag cacgagcgca
     5221 taagcaatcg ttagaaagag atattggttt aagaaatgag cgtttagctc aacttaaaga
     5281 agaagcgact caattagagc cagttaaaca atctgatatt gacgccttca ttagtttgaa
     5341 tcaacaagaa aatgaaatta aaaataaaga atttgaactt actgcaatcg aaaaggatat
     5401 tgcgaataaa caacgtgata aagatgaatt gcaagcaaat attggttggt ctgaaacgca
     5461 tcatgacgta gatagttcag aggcaatgaa aagttatgtc agtgagcaaa tcaagaataa
     5521 acaagaacaa gctgcataca ttaaacaatt agaacgtagt ttagaagaaa ataaaatcga
     5581 agataatgcg gttcatagcg aactagattc tgttgaagaa aaaatagttc ctgaagagac
     5641 ttttgaaaag aaaaaagaat actcacaaca agtcattgaa ttaaacgaaa aagagaactt
     5701 gtatagcaaa ttgaaagaac gtttcgaaat tgagcaacaa gaaaaacaaa aacgtcaaaa
     5761 attgttacga acaacgttta ttcttttaac attagtaggt attggtttaa ctgccttctc
     5821 atttatttca aacaacatgt tgtttggtat tattttcgca gtattaactt tagtatttgt
     5881 tatcggtatc atcatgtcta aatcaaaaga agtagattat agcgaggcca tcacagatga
     5941 aatcgaagaa attaaagcac aactagccat tttagatgaa aattatgact tagattttga
     6001 tcttgatgag caatatcgca ttcgtgatca ctggcaacaa gctttgaaaa ataaagatat
     6061 tttagaagag aaacgtcagt atattgaagg tcgtttaaat gatgcgaaag ggcgtcatga
     6121 tgaattacaa tcgacagttg agaacgttaa ggatgaatta tacttatcat ctaaaatttc
     6181 aaatgattta attgttgata gtatttctac aatggcgaat attaaagcat tagatcaaca
     6241 tatcagtgat ttaaatcaac aacgccagca attggttcaa gaattagata cattctacaa
     6301 tcatgctgaa gctgttacaa aatcacaatt tgtttatttc aacaaattat ctttattcca
     6361 cgatgtgcaa caatggttga aatcagctga agacacgaat gaaaaatggc gtattaatgc
     6421 tgaaaatacc aaacttgtta caaatgaatt aaatcattta aatgctcaat tagaagaaaa
     6481 taataaagaa attacagcac tattcgattt tatcaatgtt ggtactgaag aagattttta
     6541 ccaacatcat gaagattacc aaacttatac tagtaatttg agtcgtttta atgatttaac
     6601 taaatatctt gagaaccaaa actattctta tgaattaagt tctagtttaa gtgaaaaaac
     6661 aactgcacaa cttgaagaag aagatcattt attggctact caagttgacg aatataatga
     6721 gcaatatctt gaaatgcaag cacaagtcag tgatttaagt gcacaaatca atcacatgga
     6781 aactgataca acgcttgcta atttaagaca tgaatatcac agtcttaaaa atcaacttaa
     6841 tgatatcgca aaagattggg caagtttaag ttatttacaa agtttagttg atgaacacat
     6901 taaacaaatt aaagataaac gtttgcctca agttattaat gaagcggtag aaatattgaa
     6961 gcatttaaca gatggcagat atacgatgat taactataat gaagattcaa ttacggttaa
     7021 acatgttaat ggtcaattat atgatcctgt tgaactaagt caatctacaa aagaattact
     7081 ttatgtagct ttacgtatca gtttaattaa ggtactaaga ccatattatc cgttcccatt
     7141 aattgttgat gatgcatttg ttcattttga taaaaaacgt actgaaaaaa tgttgaatta
     7201 tttacgatca ttatcagaac actatcaagt actatacttt acatgtgtaa aagataatat
     7261 tgttccatca aaagaagtga ttacattaaa caaaatagag gaaggcggga aacgatgaga
     7321 aatatagaga atctaaatcc cggagattca gttgatcact ttttcttagt gcataaagct
     7381 acacagggtg taacagcaca aggtaaagat tatatgacat tacatttgca agataaaagt
     7441 ggtgaaattg aagcgaaatt ttggacggct acaaaaaatg atatggcaac aatcaagcct
     7501 gaagaaattg tacatgttaa aggtgacatc ataaactatc gcggaaataa acagatgaaa
     7561 gtcaaccaaa ttagactagc gacaactgaa gatcaattaa aaacagaaca atttgtagat
     7621 ggtgcacctt tatcaccggc agaaatacaa gaagagattt ctcattattt gctagatatt
     7681 gaaaatgcta atttacaacg tatcacacgt catttattga aaaaatatca agaacgattt
     7741 tacacatatc cagctgctag ttctcatcat cataactttg cgagtggctt aagctatcat
     7801 gtattgacga tgttacgtgt tgcaaaatca atttgtgaca tttatccatt gttaaacaaa
     7861 agtttgttat atagtggtat tattttgcat gatattggta aagttagaga attgagtggt
     7921 cctgttgcga cgtcgtatac agtcgaaggt aacttattag gacacatctc gattgcgagt
     7981 gatgaagtag ttgaagcagc tcgtgaattg aacattgaag gagaagaaat catgttgtta
     8041 agacatatga ttttatctca tcatggtaag ttagagtatg gttcgccaaa actgccatac
     8101 ttaaaagaag cagaaatttt atgctatatc gataacatcg atgctagaat gaatatgttt
     8161 gaaaaggctt ataaaaaaac tgacaagggt cagtttacag ataaaatatt tggtcttgaa
     8221 aatcgtagat tctacaatcc tgaatcactc gattaagaat taaaagatat cggacagatg
     8281 agtatactga aacatgagct catctgtccg atttttaatg cgaaaaaacc gagcggccgt
     8341 ggtatgattt ttgaaccacg gtcgctcggt tttgtgttat tggctcatgc cggattgtcc
     8401 gccttgtgcg ccaccttgtt taagtttttc agggtttaag attttatctt cgacaactga
     8461 tttaatatca cgatctttaa agtcaacatc gtattctttt aatagatctt tgtatgcatc
     8521 agttaataat tttggatttt tttgtacttt ctgatcgact aatttttctt tcaggctttg
     8581 tttttcactg ttaaagtctg ttggtttatc agctttaata atatgatatc caaagcttga
     8641 tttaacaacc tctgatactt caccatcttt aagcttaaat agtgcttttt caaaatcttt
     8701 atcagtttgt cctttaagaa cataacctaa ttcgccatct tttttagctg aaccagtatc
     8761 cattgattct tttttagcga tttcaccaaa tttacttgga tcttttgaaa cttctttttg
     8821 aatttcttca gctttttgtt tcgcttcttt atcgtctaag ccttctttgt cgcttttctt
     8881 agatttaact ttaattaaaa tgtgtgaagc tttcttgctg tcttctttaa tttcagaatc
     8941 agagatttta attttatctg atagtaattc tttatgataa gcagcagtac gtaaattttc
     9001 tttatattta tcggctgtta aaccttgctg ttgaagggcc ttttcaaatt tatctttacc
     9061 gccgtattgc ttttgcattt tttcaatttg ttcgtcaatc ttcttatcat taactttatt
     9121 tttatattta tcagctaaaa ttttatttaa catttcagta aatgatgcat ttgcaatttg
     9181 atctttaccg atttttttca ttgtatctgc aactgttacg tctccagctt tagaagaaat
     9241 taatgtattt tctttagagt ctgtggcact agcgccacaa gcgcctaata ataaagcact
     9301 agctgttacc ggaacgatta atttgtttat catcttcata gttgaaactc ctttgtaagt
     9361 taatcatcgt gaatataaca tagttttata ttatcaccaa tcaataaccc atgacatcag
     9421 actagagcat taggtttagg ggtagaaatt gatgaaaata aaaaaacaag gtctatatac
     9481 atttgattgt aagctattac atagcctaaa aacaaataat aacaccttgc tataattatt
     9541 ttgttataaa tgatatggat tttta
//
LOCUS       DAMZLE010000094         9410 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMZLE010000094 DAMZLE010000000
VERSION     DAMZLE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.095"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..858)
                     /locus_tag="POB92_001963"
     CDS             complement(<1..858)
                     /locus_tag="POB92_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDI6579436.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGFKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLK
                     NGKSKLVNDN"
     gene            complement(979..1698)
                     /gene="mecA"
                     /locus_tag="POB92_001964"
     CDS             complement(979..1698)
                     /gene="mecA"
                     /locus_tag="POB92_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDI6579437.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(2069..2464)
                     /gene="spxA"
                     /locus_tag="POB92_001965"
     CDS             complement(2069..2464)
                     /gene="spxA"
                     /locus_tag="POB92_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDI6579438.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            2759..3748
                     /gene="trpS"
                     /locus_tag="POB92_001966"
     CDS             2759..3748
                     /gene="trpS"
                     /locus_tag="POB92_001966"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI6579439.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(3790..4671)
                     /locus_tag="POB92_001967"
     CDS             complement(3790..4671)
                     /locus_tag="POB92_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579440.1"
                     /translation="MQNKSKSPFKIALSRFIHNKIAMLSIIFLLIITIISIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(4683..5645)
                     /locus_tag="POB92_001968"
     CDS             complement(4683..5645)
                     /locus_tag="POB92_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579441.1"
                     /translation="MVKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSISHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPALTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            complement(5638..6618)
                     /locus_tag="POB92_001969"
     CDS             complement(5638..6618)
                     /locus_tag="POB92_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDI6579442.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGDEHQVACFYVNKVGDLNG"
     gene            complement(6621..7607)
                     /locus_tag="POB92_001970"
     CDS             complement(6621..7607)
                     /locus_tag="POB92_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579443.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKMDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            complement(7658..9373)
                     /locus_tag="POB92_001971"
     CDS             complement(7658..9373)
                     /locus_tag="POB92_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6579444.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTEASKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNENLLTIDKKIRPKSYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKA
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVTN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAADPKELNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     DKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
BASE COUNT         3091 a         1645 c         1232 g         3442 t
ORIGIN      
        1 attgtcattt actaatttgc tttttccatt ctttaaccat aaatcaactt gtaattgcca
       61 ctcaatagga tgatttttaa tataaatttg ctctggaaaa atataaccat aattgtgcac
      121 tacttcttga tcagttaact gtaattgata cattgcactt aaagtgggtt ctaaaacaga
      181 attttgccag cgacaatatt tgatatattg attaattgcg aactttgata atttataaac
      241 agcatgataa tcacaaggcg cctcattaaa caactcacta attttggcat ttcttttttc
      301 agcgacatat ttgtgcccgc ctaaatattg tatttgttga aacatcatta tttgtttttt
      361 ctctaaattg aacgtatgaa gttttcgagt gaatggatta ataaacatac tttgaaaatg
      421 attgaacttc actttattat gacaataatc aacatcattt aataaccaac ttactttata
      481 tcccattttc tttaaacctt ccgttcgttg aagaatctgt ttataaggaa ttggcgaata
      541 ctgtagctca ataacatatt tattattaat caaaatatcc ggaatttgtt ttatttcttt
      601 taaaaatggc tctatctcta ctttaaatcc ttgttgctgt atcatctgtg ctaaaatcaa
      661 tttactttta taatgtttaa tcgtttcatt attaaaacat ttttgttccg ctaaatgttt
      721 atgtgcaaaa tgactaatta ctttgagccc acgctttaaa ataacttgct tgccacacac
      781 cggacaaaaa tattgtgtct ttctcaatgc agtagttgct aacacacgtt ccttttcttc
      841 atttaaagct actaacatat cttcacctca aaatattaca cgtaagttta actactatta
      901 ctttttcagc ttttgaaatc aataaaaaaa gagactccaa agagtctcct ttcatagtgc
      961 gttgcttaat taatatcgtt attcagttgt ctctggaaaa taacgtcgaa cttgagctgt
     1021 tacgttatga ctcataatta ttttagcata gtcatttaaa taaacttctg ttctgtctgt
     1081 tggataagca aattcaagca attgactgta actatcatta atgacttctt gatcaacatg
     1141 actatcaaaa tgtacagcat aataataagt accatcaacc atatataaca aatcttcaaa
     1201 ctctgtagtt attggattgc tatgatatgc ataattaata acatcttcta aatcgttaaa
     1261 tttcacaata attgttcttg tatttttacg tgctgaagac ttttgacgtt tagaaccttg
     1321 agcttctttt tcttttgttc gttgctcgaa taattcttct aattgatctt caccttctaa
     1381 tgtttgagct aacaattctt gaacttgttc atcaaattga tcagttgcat catcatcaga
     1441 catattcatc atatcttcat ttttagattt agaaattgtg acttcgacac ctttttcaaa
     1501 ggcatgtact tgaatccata atggaccttc tacaacaaaa tcttcttctt cgttaatttc
     1561 atccatcatt gaccaaaaga attcttcgcc acgtttgcga tttgtccata aatcttcacg
     1621 actaaatcca cgggcctcga tatcgctata tgttataaac aattttacag ttgtatcatc
     1681 tactcgttct attctcatat catctcactc cttacagtcg atgaatagta tccacattgt
     1741 attaaagcat caaacaaaat acaatttatt tgtttgattt ctttctctaa aatattttgg
     1801 taacttataa taacatattc gttgtagcaa aactatttaa atgatttata cagttaaagc
     1861 attaaagcac tttcttaatt taattttacc ttatcatagc aaatgtttca tccaaaacac
     1921 tctaatatag agctatatta acaattgcac atttgattta aaagatacta agcaattatt
     1981 gtaaaattaa aaccaaaaaa gcaacactat agaaatagtg ttgcttgaca tatcttttag
     2041 taaagtatga cacatagtta aatggttatt agtcaaccat acgttgtgct tcttgtaatt
     2101 ggaacgtacg aacttttcta ggtaagaaac gtcgaatctc gtcctcatta taaccaactt
     2161 gtagtcgttt attatctaaa ataattggac gacgtaataa gccaggatta tcttgaatga
     2221 ttgaatataa gtcttgtaat ggtagtgaat caatatcaac atttaatttt tggtatgttt
     2281 tagaacgtgt agaaatgatt tcatcagtac cgtcttcagt catttttaat atttgcttaa
     2341 tttcatcaat tgttaaatgt tcagaaaaaa tattacgctc cgtatacgga atgtcatgtt
     2401 cttgtaacca tgctttcgct ttacggcaag atgtgcaact tggtgaagta aataatgtta
     2461 ccatacatct cactctccta tttgaatgaa taaaattcat tgcttaaaat ttagttatag
     2521 atcaagaaaa aacatttttt tctaaaattc ttaatcgtta ctatttatta taactatcta
     2581 acattaaaat taaatgagaa aaacctaatt tttcagataa gtttctacac ttataaaaaa
     2641 gattattaat ccctttgtta gtagtaagtt atacgtatat tctaacacat cttacatttt
     2701 taagaaatac tgttataatg ataattatta aaatattact aagaaagtag gcatttaaat
     2761 ggagacatta ttttcaggca tccaacctag tggaattcct actattggaa attatattgg
     2821 cgcactaaaa caatttgttg atgtgcaaaa tgactatgat tgttatttct gtatcgtaga
     2881 tcaacatgca attacaatgc cacaagatcg tttaaaatta cgtaaacaga ccagacaatt
     2941 agcagcgatt tatttagctt ctggtataga tccagacaaa gcaacattgt tcatacaatc
     3001 tgaagtccct gcacacgtac aagcaggatg gatgttaact acgattgctt ctgttggaga
     3061 attagagcgt atgacgcaat acaaagataa agctcagaaa gcagttgaag gtatacctgc
     3121 tggtctatta acatatccac ctttaatggc agctgatatt gttctttaca atactaatat
     3181 cgttccagtt ggagatgacc aaaagcagca tatcgaattg actcgtaacc ttgtagatag
     3241 atttaatagt cgctataatg atgtgcttgt gaaacctgaa attcgtatgc ctaaagttgg
     3301 tggacgtgtc atgagtttac aagacccaac aagaaaaatg agtaagagtg atgataatgc
     3361 taaaaacttc atttcattat tagacgagcc gaatgttgca gctaaaaaaa ttaaaagcgc
     3421 agtaactgat tcagatggta ttattaaatt tgatcgtgac aacaagccag gtataacaaa
     3481 tttaatttca atatacgctg gattaacaga catgccaatt aaagatattg aggcaaaata
     3541 tgagggcgaa ggttatggta aatttaaagg tgaccttgct gaaatagtta aagcattttt
     3601 agtagaattc caagaaaaat acgaaagttt ctataactca gataaacttg atgatatttt
     3661 agatcaaggt agagataaag cacacaaagt ttcatttaaa actgtcaaaa aaatggaaaa
     3721 agcgatgggt ttaggacgta agagataaaa taaagaggta ctagaattaa ctagtgcctc
     3781 ttttattatt taacgtctac ctctaggatt aaaagcatct ttaagccctt cccctacaaa
     3841 gtttatactt aatatagtta atgtaatcat tatagcaggt ggcatccata tccacggttt
     3901 accacttata acatcccctt cttgagcatc actcaacata ttaccccaag atggtattgc
     3961 tttactaatt ccgaatccta aaaagctcaa tcctgattcc actacaatca taccggcaaa
     4021 taacaatgtt gcttgtacga taactacact taatatattc ggcaaaagat gtttcaaaat
     4081 aattttatat gtgggtgtac caattgattt tgctgccaaa aagtattcat tttctttttc
     4141 ttgaagtact ttaccacgaa caagtcttgc aatacctccc caacttaata cgataataac
     4201 tagaacaaga attatggcag atccataagg atttttaatt ttatctccaa gtgcagcatt
     4261 taatacaatt gcaaatatta aaaatggaaa taacataaca aattcggtta ttctcattaa
     4321 taatgtatca acaatacctc caaaataacc agaaatcact ccaactgtaa ttccaatcaa
     4381 aagcattcct attgtagatg taattccaat ggataatgaa atacgacctg catataacaa
     4441 acgactaaag ttatccctac caccagagtc tgtaccaaga atgttttgtg ctgtcatttc
     4501 accttttata tttaataaat cttgttggtt cactggaaaa ggagctatta atggcgctat
     4561 aattgatata atagttatga ttaataaaaa aataatcgat aacattgcaa ttttattatg
     4621 aataaatcta gacaatgcaa ttttaaaagg cgattttgac ttattttgca ttccaatccc
     4681 ccctagttac ttctaattct tggatctata aatccataag taatatcaga aatcaaatta
     4741 cccagtaaac ctaaaaatga gaaaaacaac gttaatgcca tcattaatgg atagtcttga
     4801 cctattaccg attctaaaaa taatttaccg ataccgttat atgaaaagat agtctcagta
     4861 atcacagctc cacctaaaat acttacaata tcagcaccta aaaatgtaat aataggtatt
     4921 aaagaatttc tcaatatatg tttattataa attttattca tagataatcc ttttgacctt
     4981 gccgtcaata cataatcttt tttagaattt tcaataatat cattacgtaa atattgaata
     5041 taaccagcag tagataataa tcctaaagtt aatgcaggca aaaatgtatg ataaatttta
     5101 ctcatataat attcaaaagt accttcttta aggttaatat caacagaacc ttgaaacgga
     5161 aaccattgta attcaaaaga aaaaataaaa attgcaaata ctcccgcaat aaaagatgga
     5221 atagctaaca tcaaataatt aaatatttgc acagtataat cagtcaaact gtaagaatat
     5281 ctacctgacg ttattcctaa agcaaatgag ataatataag taataattaa tgacatagca
     5341 ccgagtaata ttgtatttgg aattctttcc tcaataacat caattaccgg ccttttatat
     5401 ttgattgact cccctaaatc accatgtata acatgattga cccatctaat gtattgatgt
     5461 gatatagagt cgttgaggcc taacttttcc ctttgtgcat ttatcgcttc ttgttttatt
     5521 tttccgtttt gtaaatctga aaatggatct cctggttgaa tgatagctaa tgaaaataca
     5581 acaatagaaa ttaaaattag taacggaatc attaaaccta atctctttaa tattaattta
     5641 accatttaaa tcacctactt tatttacata aaaacaagca acttgatgtt catcaccaat
     5701 gtcctttaat tgtggtatat tttctttaca tttatctttt gcaatcggac atcttgtatg
     5761 aaaaggacaa ccttgcggcg gattacttgg cgaaggtaat tctcctttta atataattct
     5821 attgttatta tgtttatcaa tttgtggtat tgatgaaatc aacgcttttg tatatggatg
     5881 tttgggattt tcataaattt ctttatcaga tgcgatttca actatatgac ctaaatacat
     5941 aactccaatg acatcactta tatgttttac tacacttaaa tcatgtgcga taaataaata
     6001 gcttaagtta aattgttctt gtaaatcttt taataaattc agtacttgag attgaacaga
     6061 tacatctaat gcacttacag gctcatcagc aacaattaaa ctcggacgca aagccaatgc
     6121 tcttgcaatt cccactcttt gtctctgtcc acctgaaaat tcatgtgcat atttataata
     6181 tgcatcttca cttaggccaa cacattttaa taaatatagt acttcttttt ttatttcttc
     6241 ttttggcaat tttttataat ttaaaatagg ttctgaaatg atatctccaa ccatttgcat
     6301 cggattcaat gatgcatacg gatcttgaaa tatcatctga tattgttgtc gtgattttct
     6361 gagtttttta ccttgtaatc ttgttatatc ttcaccatta acaattattg agcctgaagt
     6421 tgcatcttca agcctgataa tcactttacc taacgttgac ttaccacaac ccgattctcc
     6481 tactaatcca aaagtttgtc ctttttttat tgaaaacgat acgtcatcaa cagctttaac
     6541 gtactgagtt ttttgtaata atccagtttt aattttgtag tattttttta tttggttgac
     6601 ttctaaaata ttttccattc ttatatctcc ttatattcat ttttgtacaa atgacaacgt
     6661 acaatgacgt cttcacaaac atgcatttta ggagattgat tattacaaat atccattttt
     6721 ttgttacatc tatttgcaaa tctgcactta ttaacgtgaa atgcttggat tgatggcaca
     6781 attccttcta tcgtttcaag tcgtttctgc tcttctttaa gttttggaat tgttgataat
     6841 aatttttggg tataaggatg tttcggattg tgtagtattt cttttatgcc tccaaattct
     6901 acaatttgac ctgcatacat aactaagact ttatcgcaaa actcagcaac tacactcaaa
     6961 tcatgtgtaa tcatcataat tgccatttgc gtttcctttt gcaattcttt taataagtct
     7021 aatatttgcg cttgaatcgt gacatccaat gctgttgtag gttcatcagc aattaataat
     7081 ttaggagaac atgaaatggc cattgcaatc attactcttt gtctcatacc accagataat
     7141 tgatgaggat agctatttaa tatttcatct tgtctagcaa ttccaacttt ttttagtaaa
     7201 tcttttgcta agttatttgc ttctttttta gaaatttttt tatgtgattt tatagattcc
     7261 acaagttgat tttttatggt aaatacagga tttaaagcag tcataggttc ttgaaaaatc
     7321 atagcaatat catttcctcg aatatctagc aattgcttct cattgagcgt atcgattcgt
     7381 ttaccatcaa atataacttc tccattactt agtttagata tcttttctgg taataattta
     7441 ataattgact tacttaatat ggacttaccg caaccagatt cccctactat acccaaaact
     7501 tctcctttag ataattcaaa agaaatattt tcaacagttg ctaaccattc attatttatt
     7561 tttaatgatg tttctaaatc tttaacctct aacaatacat tattcataac atccccccat
     7621 ataagtaaac ctcgacaaaa tcgtcgaggt tacattacta agcttcttta gttaaattat
     7681 ataaactttg atcagttcca atttcaatgt ctaagtttcg tactttatca ttaactatag
     7741 ttatcgattt accttggaac attggtaatc caggtaactc atcattcata tatttttgcc
     7801 atttaacata aatatcttta cgtttctttt catcaatgcc tactttatcg aagtctaacg
     7861 catcatctaa atattgatct gattttggta aaactgttct catttcattt tgaggtctat
     7921 cagtgtggta taaatctgat gggtctggat ctgtaccacc tgcccatgat ctgaagtaca
     7981 cttccatatc ttttgatgca tttgctaagt cttcattata tttaccgaat tctactaact
     8041 tcacatttgt tttcaagcca actttttccc agaaatcttt tatcgcagca gttcttggtt
     8101 caaaagtagg atttgaacct gaataatgtt taaagttaat ctcaaattta ttacctttag
     8161 gatcttctct aaatccgtca ccatctcgat ctttataacc taacttatct aagatttttt
     8221 tagctttttc aggatcatat ttataatcgt ttagttcctt aggatcagct gctatccaat
     8281 gcattgatgg tacaaaacta tttatttcac tagcgtaacc attgaaaaat gctttgatcc
     8341 atttttctct atcaattgca taaagcattg ctttacgtaa ttctttgtct tcatattttg
     8401 gtctcacttt accagtttta ttagcttttt tatcgtaatc atgagatacg aatcctatta
     8461 aaccgtagtc taagcttggc gcagataata ccttgagacc agcattagat gacttagcat
     8521 cttttgccat tgcaccggta gcatcattcg taacatcaat atcgcctttt tccattgcct
     8581 taataatttg agcttgatca ataactttta aattgatttt gtctaatgca ggcttacctt
     8641 gccaataatc atcaaattta acaagttgaa cagcctcacc tgggacgatt ttcttaactt
     8701 tatatggtcc aataccaata ggatattttc ggattttatc tgatttcgct aaatctttaa
     8761 ttggtacatc tgataaatat tttttactta ataaaggtcc agtaataaat cctgttaagt
     8821 aattttcttg ctttttatca aatgtaacct gcattgtgta gtcatctatt ttcttcaatc
     8881 cgctaatatg atcagctttt ccttcatgat aatcttttgc accttggata ttttctacac
     8941 ttggataata agcaccttcg taatccttgt tagctaagac ttcaattgaa taaatccaat
     9001 catcaatttt caattcatta ccatcatgcc atttaatacc ttttttaatt ttaaattcta
     9061 ttttcttagc cggctcgatg tccttccatg aagcaatata tgatttagga cgtatttttt
     9121 tatcaattgt taataagttt tcattaaaat atccttcaac tacagcatcc gcatgtgtac
     9181 tatttaataa cgaagaataa acaccacttg gcggtgctgt taacgctaca tttagcgtac
     9241 cacctttatg ttttgacgct tctgttttag tagcatcttt tactccttca tctttattac
     9301 tgctttttcc gcaagcgctc aacactaaaa cgacaataag aagtattgaa atatatttaa
     9361 taagcttccc catacatatg cctcctactt tcaatctaat tttttctttt
//
LOCUS       DAMZLE010000095         9135 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMZLE010000095 DAMZLE010000000
VERSION     DAMZLE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.096"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            338..967
                     /locus_tag="POB92_001972"
     CDS             338..967
                     /locus_tag="POB92_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDI6579445.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKPDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            1664..2677
                     /locus_tag="POB92_001973"
     CDS             1664..2677
                     /locus_tag="POB92_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDI6579446.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            2730..3740
                     /gene="gap"
                     /locus_tag="POB92_001974"
     CDS             2730..3740
                     /gene="gap"
                     /locus_tag="POB92_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6579447.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            3879..5069
                     /locus_tag="POB92_001975"
     CDS             3879..5069
                     /locus_tag="POB92_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI6579448.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            5191..5952
                     /locus_tag="POB92_001976"
     CDS             5191..5952
                     /locus_tag="POB92_001976"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI6579449.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            5955..7472
                     /locus_tag="POB92_001977"
     CDS             5955..7472
                     /locus_tag="POB92_001977"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDI6579450.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            7602..8906
                     /gene="eno"
                     /locus_tag="POB92_001978"
     CDS             7602..8906
                     /gene="eno"
                     /locus_tag="POB92_001978"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6579451.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
BASE COUNT         3278 a         1420 c         1803 g         2634 t
ORIGIN      
        1 aacttttaac acaacactta aaccaatgct ttaattttca atacgtagct ataatttgtt
       61 gtaaaatcaa aaaggttaaa atgttaattt tcaaaaaaag gctcaaaata tgtttgattt
      121 agttattaaa tgttaagata tataagacta ctatttcttt gtaaaaatga atccgattta
      181 cgagtgagta atagtgaagg cagttttaag ttgaagaagg caaaaagagt aaatgtttat
      241 taatatttgt agaaactagg taagcaaatt agttgtgaaa atgttaatgg ttgcgtgata
      301 atttctatat ttaaattagt ttgaagtgag ggagagtatg tcgaatcaaa attacgacta
      361 caataaaaat gaagatggaa gtaagaagaa aatgagtaca acagcgaaag tagttagcat
      421 tgcgacggta ttgctattac tcggaggatt agtatttgca atttttgcat atgtagatca
      481 ttcgaataaa gctaaagaac gtatgttgaa cgaacaaaag caggaacaaa aagaaaagcg
      541 tcaaaaagaa aatgcagaaa aagagagaaa gaaaaagcaa caagaggaaa aagagcagaa
      601 tgagctagat tcacaagcaa accaatatca gcaattgcca cagcagaatc aatatcaata
      661 tgtgccacct cagcaacaag cacctacaaa gcaacgtcct gctaaagaag agaatgatga
      721 taaagcatca aaggatgagt cgaaagataa ggatgacaat gcatctcaag ataaaccaga
      781 tgataatcag aagaaaactg atgataataa acaaccagct cagcctaaac cacagccgca
      841 acaaccaaca ccaaagccaa ataataatca acaaaataat caatcaaatc agcaagcaaa
      901 accacaagca ccacaacaaa atagccaatc aacaacaaat aaacaaaata atgctaatga
      961 taagtagtat ttagtcaaac aaaaatgaac cagtatgaca gacaacacaa ttaattaggt
     1021 tgtctcgaat attggttctt atttttataa ttgttaatta ggggagagat gatacttaaa
     1081 tagttagttg tttattttac ggaaagtgaa atttattttg agtgaggtgg gacagaaatg
     1141 atattttcgc aaaatttatt tcgtcgtctg accccaactt ccacattatt gtaagctgac
     1201 tttccgtcag cttctgtgtt ggggccccgc caacttgcac attattgtaa gctgactttc
     1261 cgccagcttc tgtgttgggg cccctgactt taattgaaaa atgcttgtta caagtgcatt
     1321 ttcgttcggt cactactact aatgtgactt ttcggattct agagcattga tttatgtctt
     1381 agttcaaata ataagcaaat gattagcgag tcagtataag ggtatacatt tgactacagc
     1441 gaataaaata aaacgttttg ttgaataaca aatcgaaaat atattgcaag cgctttatca
     1501 aattatttag aaaatttaag ttttatgctt gcaatttttg aaatagaata gtactattgc
     1561 aagtgtaaag aggttaattt ttgtcccacg cgggacttaa aaaggcaacc actggttgtg
     1621 acatatcctt atttacattt ataaatataa ggaggaggta gtagtgaaag acttattgca
     1681 agcacagcaa aagcttatac cggatctcat agataaaatg tataaacgtt tttctattct
     1741 tactactatc tcaaaaaatc agcctgtcgg acgtcgaagt ttaagcgaac atatggatat
     1801 gactgaacgt gtactgcgtt ctgaaacaga tatgcttaag aaacaagatt tgataaaagt
     1861 taagcctacc ggaatggaaa ttacagctga aggtgagcaa ctgatttcgc aattgaaagg
     1921 ttactttgat atctatgcag atgataatcg tctgtcagaa ggtattaaga ataaatttca
     1981 aattaaggaa gttcatgttg ttcctggtga tgctgataat agtcaatctg ttaaaacaga
     2041 attaggtaga caagcaggtc aattacttga aggcatatta caagaagacg cgatagttgc
     2101 tgtaactggc ggatccacga tggcatgtgt tagtgaagca attcatttat taccatataa
     2161 tgtattcttc gtaccagcca gaggtggact aggcgaaaat gttgtctttc aggcaaacac
     2221 aattgcagcc agtatggcac aacaagctgg cggttattat acgacgatgt atgtacctga
     2281 taatgtcagt gaaacaacat ataacacatt gttgttagag ccatcagtca taaacacttt
     2341 agacaaaatt aaacaagcaa acgttatatt acacggcatt ggtgatgcgc tgaagatggc
     2401 gcatcgacgt caatcacctg aaaaggtcat tgaacaactt caacatcatc aagctgtcgg
     2461 agaggcattt ggttattatt ttgatacaca aggtcaaatt gtccataagg ttaaaacaat
     2521 tggacttcaa ttagaagacc ttgaatcaaa agactttatt tttgcagttg caggaggcaa
     2581 atcgaaaggt gaagcaatta aagcatactt gacgattgca cccaagaata cagtgttaat
     2641 cactgatgaa gccgcagcaa agataatact tgaataagag ataaaaagtt taatactttt
     2701 taaatatcat tttaaaggag gccattataa tggcagtaaa agtagcaatt aatggttttg
     2761 gtagaattgg tcgtttagca ttcagaagaa ttcaagaagt agaaggtctt gaagttgtag
     2821 cagtaaacga cttaacagat gacgacatgt tagcgcattt attaaaatat gacactatgc
     2881 aaggtcgttt cacaggtgaa gtagaggtag ttgatggtgg tttccgcgta aatggtaaag
     2941 aagttaaatc attcagtgaa ccagatgcaa gcaaattacc ttggaaagac ttaaatatcg
     3001 atgtagtgtt agaatgtact ggtttctaca ctgataaaga taaagcacaa gctcatattg
     3061 aagcaggcgc taaaaaagta ttaatctcag caccagctac tggtgactta aaaacaatcg
     3121 tattcaacac taaccaccaa gagttagacg gttctgaaac agttgtttca ggtgcttcat
     3181 gtactacaaa ctcattagca ccagttgcta aagttttaaa cgatgacttt ggtttagttg
     3241 aaggtttaat gactacaatt cacgcttaca caggtgatca aaatacacaa gacgcacctc
     3301 acagaaaagg tgacaaacgt cgtgctcgtg cagcggcaga aaacatcatc cctaactcaa
     3361 caggtgctgc taaagctatc ggtaaagtta ttcctgaaat cgatggtaaa ttagatggtg
     3421 gtgcacaacg tgttcctgta gctacaggtt cattaactga attaacagta gtattagaaa
     3481 aacaagacgt aacagttgaa caagttaacg aagctatgaa aaatgcttca aacgaatcat
     3541 tcggttacac tgaagacgaa atcgtttctt cagacgttgt aggtatgact tacggttcat
     3601 tattcgacgc tacacaaact cgtgtaatgt cagttggcga ccgtcaatta gttaaagttg
     3661 cagcttggta tgataacgaa atgtcatata ctgcacaatt agttcgtaca ttagcatact
     3721 tagctgaact ttctaaataa ttttagtata gtttttattc aaatacgcta gtgctcagaa
     3781 ctatttagca ttaattaaag cttatgagta agcggggagc acaaacgctt ctccgcttat
     3841 ttttatataa aatttcctaa ttacaaggag gaaacaccat ggctaaaaaa attgtttctg
     3901 atttagatct taaaggtaaa acagtcctag tacgtgctga ttttaacgta cctttaaaag
     3961 acggtgaaat tactaatgac aaccgtatcg ttcaagcttt acctacaatt caatacatca
     4021 tcgaacaagg tggtaaaatc gtactatttt cacatttagg taaagtgaaa gaagaaagtg
     4081 ataaagcaaa attaacttta cgtccagttg ctgaagactt atctaagaaa ttagataaag
     4141 aagttgtttt cgtaccagaa acacgcggcg aaaaacttga agctgctatt aaagacctta
     4201 aagaaggcga cgtattatta gttgaaaata cacgttatga agatttagac ggtaaaaaag
     4261 aatctaaaaa tgatccagaa ttaggtaaat actgggcatc tttaggtgat gtgtttgtaa
     4321 atgatgcttt tggtactgcg catcgtgagc atgcatctaa tgttggtatt tctacacatt
     4381 tagaaactgc agctggattc ttaatggata aagaaattaa gtttattggt ggcgtagtta
     4441 acgatccaca taaaccagtt gttgctattt taggtggagc aaaagtatct gacaaaatta
     4501 atgtcatcaa aaacttagtt aacatagctg ataaaattat catcggcgga ggtatggctt
     4561 atactttctt aaaagcgcaa ggtaaagaaa ttggtatttc attattagaa gaagataaaa
     4621 tcgacttcgc aaaagattta ttagaaaaac atggtgataa aattgtatta ccagtagaca
     4681 ctaaagttgc taaagaattt tctaatgatg ccaaaatcac tgtagtacca tctgattcaa
     4741 ttccagcaga ccaagaaggt atggatattg gaccaaacac tgtaaaatta tttgcagatg
     4801 aattagaagg tgcgcacact gttgtatgga atggacctat gggtgtattc gagttcagta
     4861 actttgcaca aggtacaatt ggtgtatgta aagcaattgc aaaccttaaa gatgcaatta
     4921 cgattatcgg tggcggtgat tcagctgcag cagcaatctc tttaggtttt gaaaatgact
     4981 tcactcatat ttcaactggt ggcggcgcgt cattagagta cctagaaggt aaagaattgc
     5041 ctggtatcaa agcaatcaat aataaataat aaagtgatag tttaaagtga tgtggcatgt
     5101 ttgtttaaca ttgttacggg aaaacagtca caagatgaca tcgtgtttca tcacttttca
     5161 aaaatattta caaaacaagg agtgtcttta atgagaacac caattatagc tggtaactgg
     5221 aaaatgaaca aaacagtaca agaagcaaaa gacttcgtca atgcattacc aacattacca
     5281 gattcaaaag aagtagaatc agtaatttgt gcaccagcaa ttcaattaga tgcattaact
     5341 actgcagtta aagaaggaaa agcacaaggt ttagaaatcg gtgctcaaaa tacgtatttc
     5401 gaagataatg gtgcgttcac aggtgaaacg tctccagttg cattagcaga tttaggcgtt
     5461 aaatacgttg ttatcggtca ttctgaacgt cgtgaattat tccacgaaac agatgaagaa
     5521 attaacaaaa aagcgcacgc tattttcaaa catggaatga ctccaattat ttgtgttggt
     5581 gaaacagacg aagagcgtga aagtggtaaa gctaacgatg ttgtaggtga gcaagttaag
     5641 aaagctgttg caggtttatc tgaagatcaa cttaaatcag ttgtaattgc ttatgagcca
     5701 atctgggcaa tcggaactgg taaatcatca acatctgaag atgcaaatga aatgtgtgca
     5761 tttgtacgtc aaactattgc tgacttatca agcaaagaag tatcagaagc aactcgtatt
     5821 caatatggtg gtagtgttaa acctaacaac attaaagaat acatggcaca aactgatatt
     5881 gatggggcat tagtaggtgg cgcatcactt aaagttgaag atttcgtaca attgttagaa
     5941 ggtgcaaaat aatcatggct aagaaaccaa ctgcgttaat tattttagat ggttttgcga
     6001 accgcgaaag cgaacatggt aatgcggtaa aattagcaaa caagcctaat tttgatcgtt
     6061 attacaacaa atatccaacg actcaaatcg aagcgagtgg cttagatgtt ggactacctg
     6121 aaggacaaat gggtaactca gaagttggtc atatgaatat cggtgcagga cgtatcgttt
     6181 atcaaagttt aactcgaatc aataaatcaa ttgaagacgg tgatttcttt gaaaatgatg
     6241 ttttaaataa tgcaattgca cacgtgaatt cacatgattc agcgttacac atctttggtt
     6301 tattgtctga cggtggtgtg cacagtcatt acaaacattt atttgctttg ttagaacttg
     6361 ctaaaaaaca aggtgttgaa aaagtttacg tacacgcatt tttagatggt cgtgacgtag
     6421 atcaaaaatc cgctttgaaa tacatcgaag agactgaagc taaattcaat gaattaggca
     6481 ttggtcaatt tgcatctgtg tctggtcgtt attatgcaat ggaccgtgac aaacgttggg
     6541 aacgtgaaga aaaagcttac aatgctattc gtaattttga tgccccaact tatgcaactg
     6601 ccaaagaagg tgtagaagca agctataatg agggcttaac tgacgaattc gtagtaccat
     6661 tcatcgttga gaatcaaaat gacggtgtta atgatggaga tgcagtgatc ttctataatt
     6721 tccgacctga tagagcagca caattatcgg aaatttttgc gaacagagca ttcgaaggct
     6781 ttaaagttga acaagttaaa gacttattct atgcaacatt cactaagtat aacgacaata
     6841 tcgatgcggc tatcgttttc gaaaaagttg atttaaataa tacaattggt gaaattgcac
     6901 aaaataacaa tttaacacaa ttacgtattg cagaaactga aaaatatcct cacgttactt
     6961 actttatgag tggtggacgt aacgaggaat ttaaaggtga acgccgtcgt ttaattgatt
     7021 cacctaaagt tgcaacgtat gacttgaaac cagaaatgag tgcttatgaa gttaaagatg
     7081 cattattaga agagttaaat aaaggtgact tggacttaat tattttaaac tttgctaacc
     7141 ctgatatggt tggacatagt ggtatgcttg agccgacaat caaagcaatc gaagcggttg
     7201 atgaatgttt aggtgaagtc gttgataaga ttttagacat ggacggttat gcaattatta
     7261 ctgctgacca tggtaactct gatcaagtat tgacggatga tgatcaacca atgactacgc
     7321 atacaacgaa cccagtacca gtgattgtaa caaaagaagg cgttacactt cgagaaactg
     7381 gtcgcttagg tgacttagca cctacattat tagatttatt aaatgtagaa caacctgaag
     7441 acatgacagg tgaatcttta attaaacact aatattgtaa aagatgttaa gtaaacgctt
     7501 aatgacactt attttttgaa aataatagta atatcatttt gttaaatgaa agaataaagc
     7561 tataataatt atagaataac tatttaaagg agattataaa catgccaatt attacagatg
     7621 tttacgctcg cgaagtctta gactctcgtg gtaacccaac tgttgaagta gaagtattaa
     7681 ctgaaagtgg cgcatttggt cgtgcattag taccatcagg tgcttcaact ggtgaacacg
     7741 aagctgttga attacgtgat ggagacaaat cacgttattt aggtaaaggt gttactaaag
     7801 cagttgaaaa cgttaatgaa atcatcgcac cagaaattat tgaaggtgaa ttttcagtat
     7861 tagatcaagt atctattgat aaaatgatga tcgcattaga cggtactcca aacaaaggta
     7921 aattaggtgc aaatgctatt ttaggtgtat ctatcgcagt agcacgtgca gcagctgact
     7981 tattaggtca accactttac aaatatttag gtggatttaa tggtaagcag ttaccagtac
     8041 caatgatgaa catcgttaat ggtggttctc actcagatgc tccaattgca ttccaagaat
     8101 tcatgatttt acctgtaggt gctacaacgt tcaaagaatc attacgttgg ggtactgaaa
     8161 ttttccacaa cttaaaatca attttaagca aacgtggttt agaaactgca gtaggtgacg
     8221 aaggtggttt cgctcctaaa tttgaaggta ctgaagatgc tgttgaaaca attatccaag
     8281 caatcgaagc agctggttac aaaccaggtg aagaagtatt cttaggattt gactgtgcat
     8341 catcagaatt ctatgaaaat ggtgtatatg actacagtaa gttcgaaggc gaacacggtg
     8401 caaaacgtac agctgcagaa caagttgact acttagaaca attagtagac aaatatccta
     8461 tcattacaat tgaagacggt atggacgaaa acgactggga tggttggaaa caacttacag
     8521 aacgtatcgg tgaccgtgta caattagtag gtgacgattt attcgtaaca aacactgaaa
     8581 ttttagcaaa aggtattgaa aacggaattg gtaactcaat cttaattaaa gttaaccaaa
     8641 tcggtacatt aactgaaaca tttgatgcaa tcgaaatggc tcaaaaagct ggttacacag
     8701 cagtagtttc tcaccgttca ggtgaaacag aagatacaac aattgctgat attgctgttg
     8761 ctacaaacgc tggtcaaatt aaaactggtt cattatcacg tactgaccgt attgctaaat
     8821 acaatcaatt attacgtatc gaagatgaat tatttgaaac tgctaaatat gacggtatca
     8881 aatcattcta taacttagat aaataatcat tttaaacatt aagtactgcc ataattttag
     8941 ttgaggatta ttatgtcggt ataaattgaa taaagttttt gagttcacgc ttaaaaagtt
     9001 cacgcttgta tttataacct gccacagagt tgagactgtg gtaggttttt tattttgaag
     9061 tattattcat aacagactaa taatcatgag gtaactaata acacatattt aacttgtatt
     9121 cttaaactgg tataa
//
LOCUS       DAMZLE010000096         9103 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMZLE010000096 DAMZLE010000000
VERSION     DAMZLE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.097"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..228)
                     /locus_tag="POB92_001979"
     CDS             complement(<1..228)
                     /locus_tag="POB92_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDI6579452.1"
                     /translation="MTSKDITQISVIAAILTILAALKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVII"
     gene            complement(239..931)
                     /locus_tag="POB92_001980"
     CDS             complement(239..931)
                     /locus_tag="POB92_001980"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDI6579453.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYISFL
                     ESTPKQVVYERV"
     gene            complement(942..2057)
                     /locus_tag="POB92_001981"
     CDS             complement(942..2057)
                     /locus_tag="POB92_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDI6579454.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSAFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(2035..3042)
                     /gene="bioB"
                     /locus_tag="POB92_001982"
     CDS             complement(2035..3042)
                     /gene="bioB"
                     /locus_tag="POB92_001982"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDI6579455.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(3044..4402)
                     /gene="bioA"
                     /locus_tag="POB92_001983"
     CDS             complement(3044..4402)
                     /gene="bioA"
                     /locus_tag="POB92_001983"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDI6579456.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEETIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(4380..5066)
                     /gene="bioD"
                     /locus_tag="POB92_001984"
     CDS             complement(4380..5066)
                     /gene="bioD"
                     /locus_tag="POB92_001984"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDI6579457.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(5121..5288)
                     /locus_tag="POB92_001985"
     CDS             complement(5121..5288)
                     /locus_tag="POB92_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579458.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(5519..7252)
                     /locus_tag="POB92_001986"
     CDS             complement(5519..7252)
                     /locus_tag="POB92_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579459.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLYHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(7277..9040)
                     /locus_tag="POB92_001987"
     CDS             complement(7277..9040)
                     /locus_tag="POB92_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579460.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         2888 a         1699 c         1181 g         3335 t
ORIGIN      
        1 aataatcaca ttaattggat taaatacgcc aaatactagt aataataggc tagataataa
       61 tccacctaaa aagtactttt taattccaaa gaaagctaat atcaataatg ctgccggtgc
      121 agataattga aaatctaatc ctggtataat ggacggtatt ttcaaagctg ccaaaatggt
      181 taaaatcgca gcaatgacac taatttgagt aatatctttt gatgtcatac taaaacccct
      241 ataccctttc ataaacaact tgcttcggtg tgctttctaa aaatgatatg taatgattta
      301 aatcaatacg atcgtccaca aatattattc tgcctccata tctcgtatta actggtttaa
      361 tatcaaataa tcgatggtaa ccaattttag cagaggcaaa ataacctgtc gtatacgtta
      421 agtcatctga cacacaaagt tctcctttga cataaggatg cgcattgata caactagcaa
      481 ttgctaaggc atcagtcact ctttcattaa gatcaccttt attatttata tcttcaaacg
      541 aaaaatgtgt tgccctaatc cccctttgtc caaatgaatc taaacgttta ccagatatag
      601 cagatagaat aatagctcct gtataaaccg tttcattttt aatatatgtc atcccttgat
      661 ttagcgcttg ttcagtgaca ccacattctt gtgttaaatg ttggagattt gctttatcat
      721 cctcaataat ttgtaatgct tttatttgtt gaatcggttc catgattttt tgtattttta
      781 tattgagaaa atccggttgc ccattttcat gaaaaaaacc tttattataa aacctttgta
      841 ccgtctgttc tattttgtca aactcacaaa tcgtttcagc accactaata tgaacatctt
      901 gattgctaga acgcattttt atactataca tgacgatcac ctcaatcttc ttgatgcaaa
      961 atttcaaaca acctatctat atcttgttca gtatgaaaat acgacaatga tattcttaac
     1021 attggcttag tcacagttgg ataccttaaa taacttgtaa acacatgatg ctttaataat
     1081 gtttgatgaa tgttctcagc cgcttctatg tcatcaaact caataaactt aatcggcgag
     1141 tttgaactat tataattaac attgagtgct tttaactttt ggttaaaata tttactcaaa
     1201 ctatttaatt tagtgcgtct atcatcagca tttattaact tttcaatgtt tctttttata
     1261 aaatacaaat tataaattgg caaactactt gagtagatga gtggtctacc gtgattaatt
     1321 aacatatcct tcacatcatt tgaacttaaa atcacacccc cgtatgcacc acatgcttta
     1381 gataaactag aagtgagtat atctacacct tgataattcg agtaattctc tattccaaaa
     1441 ctatgtgaaa catcgagtat cagtgttgcg ttatatttat gctttaatga gactaattga
     1501 ccaatatcca caacgtcgcc attcgttgaa aatgcactat cagatatgat tatttttggt
     1561 atattttgat tagggtattt ctctaacctt ttttctaaat cagcaatatc taaatgctta
     1621 tatatcactt tttctaaacc acttaactta ataccgtcaa taatactcgc atgattttct
     1681 tgatctgaaa acacgacaca atttgtattt ttgaaaatat taaataacgc caaattagca
     1741 tcataaccac tatttaagat agtacatgca ctatatccga gccaacctgc taacattgtt
     1801 tcaatttctt cataagctgt cgaacttcca ctaattaatc ttgaacttga taagtgataa
     1861 ctatacttcc gcataaatct ttcgaaatca tccttatcaa acgctatttg acctaatcct
     1921 aaataatcat tagatgtata gttcgtacat ctcttatttt ctacttcaat atactgtcta
     1981 tctatatacc ctaccgattt aagcgaccga tataaccctt tctgttgtaa taaatcaatt
     2041 tgctcttgaa acatcattct tgttttcctt attttcacaa gtgtcataat caatttcaaa
     2101 gcccaaatca ttaatcatat cgtagtctaa ttggttcggt tgcccaccag taattagata
     2161 atcaccgaca aatattgaat tcgccgcttt taatgctaat ggctgtaacg aacgtaagtt
     2221 gacctctctt cctccagcaa tacgaatttc tttcgtagga ttgattaatc ggaataatgc
     2281 tacgattctt aaacatttca ttggtgttaa atcatccatg cttccaaact ttgtgccttt
     2341 gattggatgc aaaaaattaa tcggaatact gtcggcatcc atttctttca aagcaaatgc
     2401 catatcaaca atatcttgat tagattctcc cataccacaa atcacgccag aacatggtga
     2461 tatattattc gctttcatta gttctatcgt atctgttcta tctttataac tatgcgttgt
     2521 cacgacgtta tcatggtaat tttcacttgt attaatattg tggttatatc tgtctacacc
     2581 agctgactta agtttcttag cttgttcgtc attcgttaat cctaaacatg cacagatttt
     2641 tagttgcggg tgttgagatt taatcgttct tacagtatta ctaatatgat caacttcttt
     2701 atcgctcggt cctctaccac tcataacaat acaatatgtt ccaatatgat tatcatgtgc
     2761 cacctttgct ccatcgataa tttgttcctc tggaattaaa gcatatcgct gtttttgttt
     2821 aatatctcgt gattgtccac agtacccaca attctcagga catataccac ttttagcatt
     2881 taaaatcatg tttaatttta cttttttacc gaaataatgt tttcttaaaa tgtacgcctc
     2941 atttaataaa tctaaggtat caatattagt atcctcataa attttcaata cagtctcttt
     3001 tgttaattgt tccccttgta atatgcgttt agccaaattc atattaacac ttcctatcta
     3061 aaatgttatg tactgccttt ttaaaaatac gtaccatcgt tttcacttct ttatttgaca
     3121 tacttaacac tggcacaaac gtaatgacat tttctaaatt tctaatcatt agcccgtttt
     3181 ctttacattt acgtacaata cgttcaactt tttcaatttc taacggcgtt ttactatctt
     3241 tatctgtaac taattcaact ccaaacatta agcctcgtcc tcttacatca ccaacatttc
     3301 gatgacgctt cagcgcatgc aactgtttct caagtgttga tgatgtcgtt tcaatatgtg
     3361 acaataactt acgtttttca taaagtctta tattttctaa tgcaaccgta caaacgattt
     3421 gattcccggt gtatgtatga ccatggaaaa aggtattcac accatgcgaa tcacttaaaa
     3481 atgcattgta tattttttga gatgtcaatg tagctgcaag tggtaagtag ccacctgtaa
     3541 tcgccttacc taaacacata atatccggtt gaacatcttc atgattgcat gcaaacatct
     3601 ttccagttct cccaaaacca actgctacct catcacaaat taataagaca tcgtattttt
     3661 tgcacaattt ctcgacttct ttcaaaaagc ctttaggatg aacaaacaag cctgtcgcac
     3721 cttgaatcaa cggttccaat ataaaccctg cgatttcatc atttctttca attagaattt
     3781 gctctatatt cgttaaaata gctgtcatca tttcattttc agtatcgtaa ttactgcgat
     3841 agaaagatgg gctttctact tgaatattct caaatattaa gtctttaaat attttatgaa
     3901 aggtcttgat accaccaaca cttaccgcac caatcgtatc cccatgataa ccgtgattta
     3961 gcgttataaa cttgtttttc ttggcatatt tttctctatc aatatttttc caatactgat
     4021 atgccatctt tattgcgatt tcaacagacg cactgcctgt atcagaataa aatacttttc
     4081 ttagattact tggcgtgatt tcgattaatt tttccgcaag ttctattgac ggaatatttg
     4141 atgatcctag cagcgtagaa tgggcaattt tattgagttg ctttttaatt accttattca
     4201 agtatttgtt attatgacca tgcacattga cccacaacga tgcataacca tctaaatatt
     4261 tattgccatt cgtatcgtaa aggtaactac cctttccttt ttcaatgatg attgtttctt
     4321 ctttgctata tacacccatt tgtgtaaatg gatgccaaac atattctgag tctttttgtt
     4381 taagttgttg tgtgtaattc atttttaact cctattaatt gttttaaaaa tgcttctgag
     4441 aaattttcat acgtggcatg ttcttcaaat gtatagactg ttttatttgt taattttcca
     4501 atcgtaattt gattgtcttt ttcaatatag ctgtctgtat agcgattcat tattaaaaaa
     4561 ttactcgccg atacattctg attaacataa tcttggtgaa caatggcatc gctaatagca
     4621 cctaattttg atggcaacac actgatgaca caatctgcac aatcattgat tagatcttta
     4681 gtcatgtaga aatcatctgt accttcatat attggtacgg caattccccc agcaccctca
     4741 attaagataa aatcaaattc cttatctaaa acttttacct tatctaatac acgctgctta
     4801 tttagaaaaa tttgatctgt cattttaaat gcaaggtgtg gtgatacagg ttgcttaaaa
     4861 gtataaagtg acgttatgtc atagcttaaa tcacattcat ttttaaatac ttctaaatct
     4921 ggaaacgtcc cgtcttggcg ttcctcagtt tgaaatggtt taaaaataca aactcgatga
     4981 ccacgtgttt tcaaagcatg gtataaatgc tttgtaacat aggttttgcc tacatcagta
     5041 ttcgtacttg taataaaaat cctcataaaa aataagcacc tcaatccgaa tgtaaacttt
     5101 tataattaat aagtttacat ttaaggcgca tgtttttcaa gaaaacatga atttaaactt
     5161 ctcagtataa ctataaattt tggcaaaatt ttgaccttgc tattgaaatg actaagactt
     5221 cttaaggaat tcgggcagat ataaacagcg attcgataaa atatgtcagt taaaattaat
     5281 aattgcatta tttcttaaca aaaagacatc ctagctttac catcttgtga aacaatccat
     5341 taattgtaat attacgttga gcgccttgtt catcaatgct ataattagtt acataataaa
     5401 ttgaacatct aaatacacca aatcccctca ctattgccct agtgagggga tttattttgc
     5461 tgttgattat ctatcaccct ttttatttcc aaattctgat tccatattaa actattattt
     5521 ataatttcca gcctgctgat ttacttttta ttcttataaa gtcttgatac attcctggtt
     5581 ttcgaattaa ttcatcatga ctaccttttt gaattatttc accttcattg agcactataa
     5641 tctgatctgc atttttaata gtttcaagtt tatgtgcaat tgtaattact gttttgcctt
     5701 tacttaattc attaattgcc gtttgaatca gctgttcatt ttcagggtca atacttgcag
     5761 ttgcttcatc taaaataatt attggtgcat cttttaatat cgccctagca atagaaatcc
     5821 tttgcttttc tccgccagat aaattactac ctttttcatt tagcattgtt tgatatccct
     5881 caggtaatga catgatgaag tcgtggcaac atgcttgctt cgctgcacga ataatttctt
     5941 ctttcgttgc acctggattg ccaaacaata tgttattttc aatcgtatca ttaaataaat
     6001 acaccttttg aaatactgca ctaattttcg acattaacgt acttaatgtc atatctttaa
     6061 tatcaacacc atcgatgcga atatttccat catcgatatc ataaaagcgc aagagtaagt
     6121 gatataatgt agatttccca cttcctgaag gtccaattat tgctgttgat gtttgtgtag
     6181 gtatctcaaa attcacattc tttatcactt gtttatcatc atatgaaaag ttcacgtttt
     6241 gaaaagcgat attttcattt ttaatcgtca aattttttcc attctcatcc agttctggag
     6301 cattttttat cttttcaata tcatctatcg tcatatctat catttcaagt acgtgtgcag
     6361 cactattaat attttcaaca ctatcaaata tcacaaatga aaaaattgaa atcataataa
     6421 gggtaggtaa atcaatacta tgattcatat ataataagca tgcaactaat acaatcatta
     6481 ttgaaacaac ttttaaacta agtaaatgca ataaattgaa tgggatatat tgcatttcta
     6541 tttttgtatt tatacgttta ctttcattga ctgcttggtt aaaactttta agactcgtat
     6601 tttctttcgc gaatgatttt attacttgaa taccacgaat aacctccaat accttttcca
     6661 ctaattggtt ttgtacatta tgatacgctg gcgcattttg tcgactcttt ctttctaata
     6721 attgaatcgc aaaaaatgat agtaatacgc caatgcatgc taataatgat acttgccacg
     6781 aaactacaag tagagacaat atgagtactg taattaatat gtatccatta acaacaacgt
     6841 ccaccatttt catagcaaag ttttctaaaa aggttaaatc tgttgttact attgttgtta
     6901 actcatttga atgatgcgaa ttaaaataac ctaaccttac atttttcaat ttatccccta
     6961 tatctaaacg ttctttcgca ctcatttcat aagcgatgct ctcatggctt ttgcttttga
     7021 aatatgctgt aataaatcgt ccaatcacta ataaaaccat gataattaca acattcaata
     7081 tatctttcat ataaataggt ttatgagata gcacattatt aaatattttt gcagctaaaa
     7141 agataggtag cgcaataaaa atagcattta aaaatgacat gctaaatcct aaaatcattc
     7201 ttgctttata tggtcttatc cagtttaaaa ttttaaatgt aatttgaaac atatttgacc
     7261 tccttttctc attagtttaa ttatgtccag tattgattcc ccaatctttt gtatgcatgt
     7321 gcgtatccca cattttctta taattaccgt ttaattttag caataagtga tgtgaccctt
     7381 tttctaaaat ttgttgttta cctaaaacaa taatttgatc tgcatgttga atcgtagata
     7441 accgatgtgc aataacaatc aatgttttat cctgcgtcaa cacatttaat gcttcttgaa
     7501 ttttttgttc gttgtctgga tcaacgtaag cagtcgcttc atctaataca atgataggcg
     7561 catcttttaa tatcattctt gcaatagtga ctcgttgttt ttcaccacca gacaatttat
     7621 ctcccactgt accaacattc gtatcatatc catctggcaa cttttcaata aattcatgac
     7681 attgtgctaa cttggcagct ttttcaactg cctcatccgt agcttctgga ttgccaagtt
     7741 taatattttc tttaaaagta agatttaata aaaagttatc ttgtccaaca aaaccaacta
     7801 aatcgttaag ttgtttcgat tcaatatctt ttatatttat accgccaatc gtaatttcac
     7861 ctgaagtcac atcccagtat cgtgatataa gcttggcaat ggttgactta ccgctaccag
     7921 atgccccgac gatagctgtg aaattatttt ctggtactgt aaatgataaa tgcttaaaga
     7981 ccagatcgtc cttatctttg ttatatgaaa atccaacatt attaaaagca atttcataat
     8041 gttgaggctt cttaaatttt gttgacaata ctaactcttc tagacttaat atttgattca
     8101 cttcagttaa tgcgtattgt atagacttta aatgatttac ataattagta aaattcttaa
     8161 tcggtgctac tacacctaaa gataatacga tgcataggaa aaattccgca tagtttaatt
     8221 ggttgataga tatcaaatac atgccgaccg gtaaaatccc taaaaatgtt gaaggtaata
     8281 cactagctcc taaattcata tacccccatg tatttttaaa ccaattcaaa gtgtgaatct
     8341 tataattatc tactgcatct ttatattttt tatatgaact ttgagattga ttaaatgttt
     8401 taatcacttc aatgccttcg ataaactcta caatcgcgct attcatataa ttattcgatt
     8461 tcatttgttc agcatatgtc tcattaaatc cagacataac ttttttaaaa gcgaaaattg
     8521 aaattggtat cgttactaat aaggcactag ccatacgcca atcaatgagc attatgtata
     8581 aaaagatagc agctgacaaa agtaagtttc ctataacttc aggaatcata tgtgctaaag
     8641 gtaattctat tgtttcaacc ttatcgacaa atatattttt taattcacct attttcttag
     8701 attccactac gcctaaaggg agacgcatta atttttgagc taatttttta cgaatttcag
     8761 ataaaatttc atatgccgta atatgtgata gcatcgttga cgctccaaaa caacacactt
     8821 gtgaaatata agcgattaaa gcaataaaga tataaaccat aatcgaatta atcgtatatg
     8881 cattgttaat catcattaaa ataattttaa agactgccca atatggaact aatccagaaa
     8941 agacactgat gatagacaac aaaattgata acataatttt ccatttacta tcagaagcaa
     9001 atccgctgag tatctttatc caatttgtct gattgttcat cgtatatgta actcctttca
     9061 attaataatc taaattaagc cgcttatatt atttatttca ctg
//
LOCUS       DAMZLE010000097         8957 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMZLE010000097 DAMZLE010000000
VERSION     DAMZLE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.098"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1168
                     /gene="mnhD1"
                     /locus_tag="POB92_001988"
     CDS             <1..1168
                     /gene="mnhD1"
                     /locus_tag="POB92_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDI6579461.1"
                     /translation="YYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGST
                     KIQLQETIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIV
                     FILFIFVFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSD
                     NVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMI
                     GAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAG
                     IPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLN
                     NKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            1170..1649
                     /gene="mnhE1"
                     /locus_tag="POB92_001989"
     CDS             1170..1649
                     /gene="mnhE1"
                     /locus_tag="POB92_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDI6579462.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            1649..1942
                     /gene="mnhF1"
                     /locus_tag="POB92_001990"
     CDS             1649..1942
                     /gene="mnhF1"
                     /locus_tag="POB92_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDI6579463.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            1920..2276
                     /gene="mnhG1"
                     /locus_tag="POB92_001991"
     CDS             1920..2276
                     /gene="mnhG1"
                     /locus_tag="POB92_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDI6579464.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            2525..3679
                     /locus_tag="POB92_001992"
     CDS             2525..3679
                     /locus_tag="POB92_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6579465.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     FTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSTFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENNSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(3735..4109)
                     /locus_tag="POB92_001993"
     CDS             complement(3735..4109)
                     /locus_tag="POB92_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDI6579466.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGHVTATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(4128..5444)
                     /locus_tag="POB92_001994"
     CDS             complement(4128..5444)
                     /locus_tag="POB92_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDI6579467.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(5855..7330)
                     /locus_tag="POB92_001995"
     CDS             complement(5855..7330)
                     /locus_tag="POB92_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDI6579468.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(7461..8669)
                     /locus_tag="POB92_001996"
     CDS             complement(7461..8669)
                     /locus_tag="POB92_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6579469.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFDKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
BASE COUNT         2888 a         1602 c         1341 g         3126 t
ORIGIN      
        1 ttattatttt tcagtactat ttatgttaat aggtatcatt ggtgcattca caactggtga
       61 cattttcaac ctattcgtat ttttcgaagt atttttaatg tcatcttatt tcttattagt
      121 aattggttct acaaaaattc aactacaaga aacaataaaa tatgttcttg ttaacgttgt
      181 atcgtcatcc ttcttcgtaa tgggtgtagc gattttatat tcagtagtag gaacattgaa
      241 tctagcagat ataagcaata aattagctaa cctttcagcg catgacagtg gcttagtcaa
      301 tatcgttttc atactattta tctttgtttt tgctacaaaa gctggcgtat tcccaatgtt
      361 tgtttggtta cctagtgcat actatgcacc accaattcca atcattgcat tctttggtgc
      421 tttgttaaca aaagttggtg tttatgcaat cgctagaaca ttgagcctat tctttagtga
      481 taatgtaagc ttttcacatt atgtcatttt attcttagca ctattaacga tcatctttgg
      541 ttgtgtcggt gctgttgcat atgccaacat taaaaaaatc atcctataca atgtcatgat
      601 tgctgtaggt gtgatcttag tcggcgttgc aatgatgaca gagtcaggta tgattggggc
      661 tatttactat acattgcacg acatgttagt aaaacttgca ttattcttgc ttatcggaat
      721 tatgattaaa attacaggca ctgctgattt aagacaattt ggtggcttaa ttaaacgata
      781 tcctgtactt ggttggtcat tctttatcgc agcattaagt ttagctggca ttcccccact
      841 cagtggcttt tatggaaaat tttttattgt ccaatcaaca tttgaaagag gattttattt
      901 aagtggtgtc atcgtcttat tatcaagttt agtcgtatta tattctgtca ttcgcatatt
      961 cctacaaggc tttttcggtc aaccaaaagg atatgatctt aataacaaag tcgacgtcaa
     1021 atatttaact acgattgcaa tcgttgcagt tgtgataact gttttatatg gattgtcagc
     1081 agattactta tatccaatgg taaaagctgg tgcagaaaca ttctacaatc catcaaccta
     1141 cgttaaagct gtattaggag gtaaataaca tggctgtaca attagtatta aactttatta
     1201 tcgcagtgtt ttggttgttt gtgacaaata gttacacaac aaataacttt gtgttaggtt
     1261 tcatctttgg cctagttctt gtttacctct tacacagagt gttaccaggt agattttatg
     1321 tcatcacgct atatcgtatt attaaattag ttattatttt cttaatcgaa ctgattaaag
     1381 ctaattttga tgttttgaaa attattatta aaccttcgat taaaaatgaa ccaggattct
     1441 ttgtttatca cacagattta aaaaaagatt ggcaaattgt cttgctgtct aatttaatta
     1501 cattaacacc tggtactgta gtgcttggtg taagtgacga tcgtacaaaa atttatattc
     1561 atgccataga tttttcaact aaagagcaag aagtagaaag tattaaaaca tcattagaga
     1621 aaattgtaag agaggtgggc gaaatataat gaatcataat gttattatcg ttattgcatt
     1681 aatcatagtt gtcatttcta tgttagctat gctcattcgc gttgtgctag gcccatcact
     1741 tgccgatcgt gttgtcgcat tagatgcgat tggtcttcaa ttaatggcag ttatagcatt
     1801 attcagtatt ttattaaata ttaaatacat gattgtcgtt attatgatga ttggtatatt
     1861 agctttttta ggtactgcag tattctctaa atttatggac aaaggtaagg tgattgaaca
     1921 tgatcaaaat catactgatt agtcttgcac ttatctttgt tatcatcggt gctttaatta
     1981 gcgccctggc agctatagga ttattgagac ttgaagatgt atattcacgt gcacatgctg
     2041 ccggaaaagc atcaacatta ggtgcaatgt cattactatt tggtacgttt ttatatttta
     2101 ttgctactca aggttttgta aatatgcaat taatcgttgc gattatcttt gtattaatta
     2161 caggtcctct ttcaagtcat atgattatga aagcagcata taatattaaa acgccttata
     2221 ctaaaaagac taaagtcgat gaaatttcag aagacttaaa agacacaaaa ttgtagatta
     2281 tataacaaaa tcatgaatat aaatcaacaa caaacagcag taagatgatt ccaaattagg
     2341 aatgatttta ctgctgtttt cttttgacat tgttacctct ttttcaatgg ttttttcttt
     2401 gactacagct tcgccctatc tacatatatc tctttaattt aattgccttt catgtcgtta
     2461 tgtattatga taataataat tataaatcgt aacgattacg ctttaaaaag agagaggttt
     2521 tattatgcat tggacaatta tcggcggtgg catacaggga actgcaatcg cacaaaaact
     2581 attatcaagc ggattaacaa cagaccgatt aacaatcatt gacccacacg aaactttttg
     2641 ccaaaggttt aactcattta caaatcgaat agaaatgcct tatttaagat caccgattgt
     2701 acatcacgta catccacaac cgttccatct aaaacaattc gctaaacagc accaatatac
     2761 aaatgctttt tatggtcctt atcaacgacc tgaattgaca atgtttatgg atcatattgc
     2821 acatgcttct aaacaatatc aattagagga ttgcttggtt caaggtttag ttcaaacttt
     2881 agataaacaa gaagacaaat ggcatatcaa gttagaagat ggacaaatta tcactacaga
     2941 ttgcgtcgtt attgcaatag gtagtacaaa tattccgttt atgcctgaca ttttaaaaga
     3001 caaacagaat gtaaatcata tcttcgagaa agaacatgat caagtagtat atgataagac
     3061 cgatcatatc gttggtagtg gcattacggc tgcacatctt gcacttaaat tgttaaatca
     3121 tgataacgat aaaaagattc atttatggct aaataaagat attgaaatac atgactttga
     3181 tgctgatcct ggttggttag gtccgaagaa tatgtctaca tttttaagta ctaaaagcat
     3241 gcctgaaaga aatgccattg tacaacgtga acgtcataaa ggatcaatgc ctcacgaact
     3301 gtacttacgc cttaaaaaac atattaaaaa tggtcgtata aatgtgcata aaacacctat
     3361 cactcaaatt agtgatggtt taattaacac tgaaaataat tctgttccat atcaacagat
     3421 tatggttgca actggttttg aacaagattt tatgtcacaa ccacttatta agcaattaat
     3481 acaaaattat gatgcaccta tcaacgaatg taattaccct gttatttccg aaaaattaga
     3541 atggatacca aatctatttg tcgcaggatg ctttgcagac ttagaattag gaccatttgg
     3601 tagaaatgtt atgggtggcc gtaaagctgc cgaacgcatt gaacaagcat ttctaaaatt
     3661 acaacaatat agcgcataaa aaaactgcta gccgtagtta tgaatatctc ataacatttc
     3721 agctagcagt tcttttattt taaaggctta atagcaactg taccacgcat aactgtaatt
     3781 aattgttctt tgtcattctt aatttttata tcccatacat gtgtcgactt accttgatga
     3841 ataatttcag ctgtcgcagt aacatgacca tctttagcag aatgaatatg attggcattc
     3901 atctctaaac ctaatggaat aaatttggtt gtatcaatta gattagcaga tcctaatgaa
     3961 catgctgttt cacctaaagc aatcgaagca ccaccatgta aatatccaaa tggttgtttt
     4021 actttatcag taacaggcat agaaataaca actaaaccat cttcctggtg atctattgac
     4081 atctcaaatg tctctaataa atgagtcata ttttcacctc tttatgctta tagtaccata
     4141 gcagcaatag taccgaaaat cattaaagga atattataaa acaagaagtt tggtacacat
     4201 gtatcgcgta tatgatcatg ttggccatca acatttaatc ccgcagttgg tcctaatgtt
     4261 gaatcacttg caggtgaacc tgagtcacct aatgcactcg ctgttccaat caatgcgatt
     4321 aatgccattg tatctagtcc aattgacgct ccaaaaggaa tgaataatga tgcgataata
     4381 ggaattgttg caaatgatga tccaatacct aaagtgacaa ttaaacctat cacatacatc
     4441 atgataatgc taaataattt attatcacca gtaatacttg ttaaagtttt aactaattca
     4501 tctatatcac cagtagcatt cattacacca gcaaatccat ttgctgttaa aataactaca
     4561 ccaatataag ccataatttt aataccttca acaaacttag catctaattc ataccaatta
     4621 tatgcacgtg aaataaagaa tacgagtacc cctgccagtg caccaaaaat cattgaatct
     4681 gtaaatgttt gtactaaaaa tgtagctagt attgctacaa ttgttactat taagatatat
     4741 ggttttaact ctgtaacatt gtcactatct gaaattttac gtgtttcata ttcacgtggt
     4801 ttacgatata catataaacc gataagtaag ccaacaatat accccattga aggaataagc
     4861 attgctttcc aaatcatatt aaactcaatt gggtgattcg cctttgcaaa gccactttga
     4921 ataatttgct ggaaaatttg accgaatcca tatggtaata acacatacgg gaaacataaa
     4981 ccaaaaccga taatcaaacc gattaaacgt ctatctattt ttaagtcatt aaacagactt
     5041 aacaatggtg ggatgacaat tggaatgaat gcaatatgta caggaattaa gttttgactc
     5101 atgatactca tagctaataa tgcaatgatt attgtcactt tgactttaac tcttgaccat
     5161 cgactatttt cagcgtgaat tgcatttata atttttccta ctaaatagtc tgtgatacca
     5221 ctgtatgaaa ttaatgctgc aaatccacct aataaagcat agcttaatgc tacctcagca
     5281 ccatcgacta tatttttccc aaatacattt ataacttttt caatgctcat gcctgaaatt
     5341 aagccaccaa ctagcgcact gataaataag cttataacta cgtttaatcg acataaacat
     5401 agcacaatca ttaaaattac tgctattact actgcattta tcatatgaca accatccttt
     5461 atcactctat cgaactaaag cgtttataga ctacatagta tcagtagctt taacacctgt
     5521 caatagcaaa ctataccaat aatctttatt ttttagttaa ttgtctttct taaaataatt
     5581 ttagctttca ttaaattaaa caattttaca agcttggaac accaatcaaa atcctaagtt
     5641 ctaaaatgca atattagtag tcgttgactg aatgaacata tgcttataat atttttttgc
     5701 aatgctagtc aagttgattt atgctcacaa ggatatgcga tttatatttt cttacaacaa
     5761 tgaaaatgcc tgatacaatg cgatccttta tactttcaag agttaaatta gaaatgtata
     5821 tcaagacaca agttgtatca ggcaaataat taaattattg ttgttttaac cattgtacta
     5881 tcgtcggaat cataaatcct gttggccctt ttggaccatt atatgaagct ttattagtcg
     5941 ttgctggacc tgcaatatcg aaatgaatgt gaggtgtttg accactaaaa tgtgttacaa
     6001 aacttgccgc aaatagcgct ttaccttgtc cattcgtatg gttaactaaa tcagcgatat
     6061 cactgtgttt aatacttgca cgttcggttg cagtaatcgg taattcaaat accatttcat
     6121 cgacttcaga acttatttgt aatatatcgt ttaatatcac tttactattc gattcaaatg
     6181 cagcagcttt atcatcgcct agtgcaacaa ttgctgcacc cgttaatgta gcaaagtcca
     6241 taatcacact aggctgatat tgatttgcat aaaacacagc atctgcaagg actaatctac
     6301 cttcagcgtc tgtattcatt acttctacag tttcaccact taatgctgta aatacatcat
     6361 ctggcttcat tgatgcttca tttatcatat tttcagcaca cgcaagcact ccgacaatat
     6421 ttacaggcag ttgtaaacga ctagccgctt caatgatacc aacgacattc gcagcgccac
     6481 acatgtcaaa cttcattgta gccatgccat tcttcgtttt aatactataa ccaccagaat
     6541 catacgttat acctttacca actaaggcaa ttggtgcttc atctttgtct ttgccattat
     6601 atgtgatggt tactaatctc ggtttatgct tactaccttt acctactgct tgtaaaagtc
     6661 cgaatccttc agaaactaaa gtgtcataat ctttaacatc tactttgacc tttgtatttt
     6721 taaaatgatt aacaatatct tctgcaaatg tttgtggtgt taatacattc ggtggcatat
     6781 tactaaagtc tcttgccaaa ttaatggatt ggccaatact gataccctca tgaataaaat
     6841 ctaattcaat taatgattca ctaataaggt ttaaattcgt cttaaacggt gccttcttac
     6901 ttgatttata atgatcgaat tcatatgttg cacgctcact ttgaataccg catgccatta
     6961 atacatctga taactgatca tattttgaaa taaatgaatc cataagtaaa tacgtatctt
     7021 caatgtgttc tgactttatg tattggaaaa gatgtcccca tattttcaac atatcttgat
     7081 aactacgtgt ctttaagttt cctaaaccaa ctgtaattaa acgatatgtt tgatcttgta
     7141 catcaaatgc agttgtataa atcttcccaa ctttactacc aataatatgt tgatgtttta
     7201 gtctttcaag tgattctgta atatcgatat gattaaaact aatgcgctct aactgattta
     7261 aatgttctgg tataccaata atcaatgtat ttatttcgtt gcttagtgtg ttatttaatt
     7321 taaaattcat aatgtacctc cttagatttg atatgttact cacttcaaat tgtacaacaa
     7381 taaagccctt cagtgacact gaaggacttt ataaatgaat aatttaattt tatgtgttta
     7441 gctgaaactt tatttaaagc ctagaattta ccttttttga atgctaaacc gataccaccg
     7501 attttgaata ccgcacgtgt atcaatcact tttttcatga atgctgcttt tttaccagcg
     7561 ataggtttat caaataccat acctacaccg tcatgtgaac ctaaagaaca aacagttcca
     7621 cgatcaacgt attcgaattc ttcagttgat tcaccgttta agatgcgttt aatgttttta
     7681 gcaacacttt caccttgttg cattgcaatt tgtgctgtag ttggtaatgg acgttcttct
     7741 ccagctggga taaacgctga acagtcacca ataacaaaaa tgttgtcgta accattgatt
     7801 gttaaatctt gctttgtaac gatacgtcca cgtttaacgc cttcaaatga ttcttccatt
     7861 aatttactac cacgtacacc agctgcccat actgaagtac ctgcatttaa ttgttgtttt
     7921 tcaccatcta cttcaactac aaaacctttt tcgttacaag caacgattgg tgtagcaatt
     7981 ttaaattcaa caccgcggtc ttctaagtag ctaactgcgt ggttaactaa ttcttctgag
     8041 aacattggta acattttagg tgctgcttca acacaagtga ttttaacttt attttgatcc
     8101 acaccatatt tgctacataa ttcaggaatt ctgtctgtta attcacctaa gaattcaaca
     8161 ccagtgaatc cagcaccacc aactaagata gataaatcgt tatcatcttt ttcttttgat
     8221 gctgcatagt tagcaaattt gtcttcgata tgacgtgata attcacgtgc tgtgataaca
     8281 ttttcaattt ggaaagcatg atctttcata ccttcgatgc cgaatgtttc actaacgaaa
     8341 cctaatgcta ctactaaaat atcaaagtca taaatacctt gatttgtttc taccttttta
     8401 gcatcacggt caatttttgt tacttctgct tgaacaaagt tcactttgtc tttcttcaag
     8461 acactttcca caggatataa tacatcttca tagtttagtg tacctgctga tgcttcatgt
     8521 aaccatgttg cttcatagtg atattcattt ttattaataa gcgtaatttc tgcttcttct
     8581 gttgatatcg ctttttgcaa tttagttaca gtttgtaaac ctgcataacc agcaccaagt
     8641 acaagtactt ttttacgatc ttgagccatt taatttcacc taagctttca tattttttta
     8701 accaaatgct gataattact agtaacaact ggtcattact acttttacca caatgtatac
     8761 tagtttatca tcattttgtt atttaagtct tttaaattat gtaaaaacct gtgatgttca
     8821 caaaaaagac atttttaatc caatactaat tctatagctt ttatgttata ttttcaagca
     8881 agattcagtc agattgtgaa aaaattaatt aattttttcg tagcctgatt tcgattaaaa
     8941 atgtatccgc tatcacg
//
LOCUS       DAMZLE010000098         8830 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMZLE010000098 DAMZLE010000000
VERSION     DAMZLE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.099"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            166..1107
                     /locus_tag="POB92_001997"
     CDS             166..1107
                     /locus_tag="POB92_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDI6579470.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            1119..2363
                     /gene="fabF"
                     /locus_tag="POB92_001998"
     CDS             1119..2363
                     /gene="fabF"
                     /locus_tag="POB92_001998"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI6579471.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(2418..2789)
                     /locus_tag="POB92_001999"
     CDS             complement(2418..2789)
                     /locus_tag="POB92_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDI6579472.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            3032..3958
                     /locus_tag="POB92_002000"
     CDS             3032..3958
                     /locus_tag="POB92_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579473.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            3958..5028
                     /locus_tag="POB92_002001"
     CDS             3958..5028
                     /locus_tag="POB92_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579474.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAISSSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            5044..6126
                     /locus_tag="POB92_002002"
     CDS             5044..6126
                     /locus_tag="POB92_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579475.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            6116..7057
                     /locus_tag="POB92_002003"
     CDS             6116..7057
                     /locus_tag="POB92_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579476.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIHD
                     EANNHLRQLHEIRPQHFVFSTEEEAAQLRENKLVTQN"
     gene            7076..8731
                     /locus_tag="POB92_002004"
     CDS             7076..8731
                     /locus_tag="POB92_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6579477.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKGGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
BASE COUNT         3150 a         1276 c         1707 g         2697 t
ORIGIN      
        1 ctaaattaag cgcattaaaa ccaatttcat actgaaattt gacgataata aagcattaaa
       61 attttattaa ctagtcaata ttcctacctc tgacttgagt ttaaaaagta atctatgtta
      121 aattaatacc tggtattaaa aattttatta agaaggtgtt caactatgaa cgtgggtatt
      181 aaaggttttg gtgcatatgc gccagaaaag attattgaca atgcctattt tgagcaattt
      241 ttagatacat ctgatgaatg gatttctaag atgactggaa ttaaagaaag acattgggca
      301 gatgatgatc aagatacttc agatttagca tatgaagcaa gtttaaaagc aatcgctgac
      361 gctggtattc agcccgaaga tatagatatg ataattgttg ccacagcaac tggagatatg
      421 ccatttccaa ctgttgcaaa tatgttgcaa gaacgtttag ggacgggcaa agttgcctct
      481 atggatcaac ttgcagcatg ttctggattt atgtattcaa tgattacagc taaacaatat
      541 gttcaatctg gagattatca taacatttta gttgtcggtg cagataaatt atctaaaata
      601 acagatttaa ctgaccgttc tactgcagtt ctatttggag atggtgcagg tgcggttatc
      661 atcggtgaag tttcagatgg cagaggtatt ataagttatg aaatgggttc tgatggcaca
      721 ggtggtaaac atttatattt agataaagat actggtaaac tgaaaatgaa tggtcgagaa
      781 gtatttaaat ttgctgttag aattatgggt gatgcatcaa cacgtgtagt tgaaaaagcg
      841 aatttaacat cagatgatat agatttattt attcctcatc aagctaatat tagaattatg
      901 gaatcagcta gagaacgctt aggtatttca aaagacaaaa tgagtgtttc tgtaaataaa
      961 tatggaaata cttcagctgc gtcaatacct ttaagtatcg atcaagaatt aaaaaatggt
     1021 aaaatcaaag atgatgatac aattgttctt gtcggattcg gtggcggcct aacttggggc
     1081 gcaatgacaa taaaatgggg aaaataggag gataacgaat gagtcaaaat aaaagagtag
     1141 ttattacagg tatgggagcc ctttctccaa tcggtaatga tgtcaaaaca acatgggaga
     1201 atgctctaaa aggcgtaaat ggtatcgata aaattacacg tatcgatact gaaccttata
     1261 gcgttcactt agcaggagaa cttaaaaact ttaatattga agatcatatc gacaaaaaag
     1321 aagcgcgtcg tatggataga tttactcaat atgcaattgt agcagctaga gaggctgtta
     1381 aagatgcgca attagatatc aatgaaaata ctgcagatcg aatcggtgta tggattggtt
     1441 ctggtatcgg tggtatggaa acatttgaaa ttgcacataa acaattaatg gataaaggcc
     1501 caagacgtgt gagtccattt ttcgtaccaa tgttaattcc tgatatggca actgggcaag
     1561 tatcaattga cttaggtgca aaaggaccaa atggtgcaac agttacagca tgtgcaacag
     1621 gtacaaattc aatcggagaa gcatttaaaa ttgtgcaacg cggtgatgca gatgcaatga
     1681 ttactggtgg tacagaagca ccaattactc atatggcaat tgctggtttc agtgcaagtc
     1741 gagcgctttc tacaaatgat gacattgaaa cagcatgtcg tccattccaa gaaggtagag
     1801 atggttttgt tatgggtgaa ggtgctggta ttttagtaat tgaatcttta gaatcagcac
     1861 aagctcgagg tgccaatatt tatgctgaga tagttggcta tggtactaca ggtgatgctt
     1921 atcatattac agcgccagct ccagaaggtg aaggcggttc tagagcaatg caagcagcta
     1981 tggatgatgc tggtattgaa cctaaagatg tacaatactt aaatgcccat ggtacaagta
     2041 ctcctgttgg tgacttaaat gaagttaaag ctattaaaaa tacatttggt gaagcagcta
     2101 aacacttaaa agttagctca acaaaatcaa tgactggtca cttacttggt gcaacaggtg
     2161 gaattgaagc aatcttctca gcgctttcaa ttaaagactc taaagtcgca ccgacaattc
     2221 atgcggtaac accagatcca gaatgtgatt tggatattgt tccaaatgaa gcgcaagacc
     2281 ttgatattac ttatgcaatg agtaatagct taggattcgg tggacataac gcagtattag
     2341 tattcaagaa atttgaagca taattataaa aatcttcagt aacgttgttt tagttactga
     2401 agattttttc tgtttcttta tactaagatg agcgacagca caatcgtcat aataaaatat
     2461 aaaatattta ttaataataa agggattatc catgtagaaa caaagtaatg ctcttttttt
     2521 acctcttgtg ggttgaaaaa tggatcatca gagatagact tcttcttttt cgaagatgac
     2581 atttgatact ttaatcttct aaaaccataa cttgtcgcat caaaaatgcc ttcttgtaca
     2641 agtaaaatca aaaatatgct aataaaaata attaatgaaa cataaaacaa tatatttaaa
     2701 tatgtaatga tagtatggct attaaaaagc catataataa acgttaatat tggcgttatt
     2761 agtgccattc caagccattt tttcaacatt tgatcactcc cacttataga aaactcttac
     2821 gcatagttta cattaaaatc agacattgag gaatgatttt ttaatttctt cagctttatt
     2881 gaaattctaa aatcaatcat tcttcattag tttaaagcaa aaaaatattg atatatagta
     2941 aatattgtat atataatatt agttaagatt tcagaaaatt ttgaagggaa tggaaattta
     3001 gaaatcggaa tttgttagag gaggggatta gatggggaaa tatattttca aacgatttat
     3061 ttatatgctt atttctttat ttattattat tacaattaca tttttcttaa tgaaattaat
     3121 gccaggttcg ccatttaacg atgctaaatt aaatgctgaa caaaaagaaa ttttaaatga
     3181 aaaatatgga ttaaatgatc ctgtagctac gcagtattta cattatttaa aaaatgttgt
     3241 tacaggcgat tttggtaatt cattccagta tcataatcaa cctgtgtggg atttgattaa
     3301 accgagacta ctaccttctt ttgaaatggg tcttacagca atgttcatcg gtgtgatact
     3361 gggacttatt ttaggtgttg cagcagctac taaacaaaat tcttgggttg actatacaac
     3421 tacagttatt tcagttattg cagtatctgt accatctttt gtacttgctg tacttttaca
     3481 atatgtattt gcagttaaat taagatggtt cccagtagct ggatgggaag gtttttcgac
     3541 cgcggtatta ccgtcacttg cattatctgc agctgtttta gcaactgtcg ccagatacat
     3601 aagagcagag atgatagagg tattaagttc agactatatt ttattagcga gagctaaagg
     3661 taattcgaca atgcgtgtac tttttggaca tgcacttaga aatgctttaa ttccaattat
     3721 tacaattatc gttcccatgt tagcaagtat tttaacaggc actttaacaa ttgaaaatat
     3781 ttttggggtt cctggattag gggatcaatt cgtacgttca attacaacaa atgatttctc
     3841 agtaatcatg gcaatcacac tattatttag cacactgttt atcgtttcta tttttattgt
     3901 agatattttg tacggtgtga tagatccacg aattcgtgtt caaggaggta aaaaataatg
     3961 gctgaaaata aaaacaattt gtcgattaac gacgatcatt ctaatgcagc tatgacgcat
     4021 acctctgacg ctatctcatc atctgatttt attattagag aattagattt gaatcaggaa
     4081 cctgaaatgc aacgagaaag caaaaacttt tggcaagatg cttgggctca gttaaaacga
     4141 aataagttag ctgttgtcgg tatgataggt ttaattatca ttgtaatatt tgcttttatc
     4201 ggtccagtta taaataaaca tgattatgcc gaacaaaatg tagaacatag aaatcttccg
     4261 gcaaaaatac ctgtattaga caaagttcca tttttacctt ttgatggtaa agatgcagat
     4321 ggcaaggatg cttataaagc agcaaatgct aaagaaaatt attggtttgg tactgatcag
     4381 ttgggtcgag atttatggac aagaacatgg aaaggtgctc aaatttcatt gtttatcggt
     4441 gttgttgcag cgatgttaga tatttttatt ggcgttgtat atggtgcgat ttctggattc
     4501 ttcggtggac gtgtcgatac gattatgcaa cgtatacttg aagtcatagc atctattccg
     4561 aatttaattg tcgtaatttt atttgtatta atttttgaac catccatttg gacaattata
     4621 ttggctatgt ctatcacagg ctggttaggc atgagcagag ttgtacgtgg agaattttta
     4681 aaattaaaaa atcaagagtt tgtcatggct tcgaaaacat tgggggcttc aaaattcaaa
     4741 ttgatattta agcatatttt acctaataca ttaggtgcta tcgtggttac atcaatgttt
     4801 acagtaccta gtgctatttt cttcgaagca tttttaagtt tcattggtat aggtgtaccc
     4861 gcacctcaaa catcgttagg gtcattagta aatgatgggc gagcaatgtt attaatttat
     4921 ccacatgaat tatttatacc agcaatgatt ttaagtttat taattctatt cttttactta
     4981 tttagtgatg gattacgtga tgcatttgat ccgaaaatgc gtaaataaaa gggggcatag
     5041 catatgactg aaagaatatt agaagtaaat gatttgcatg tttcctttga tattacagca
     5101 ggggaagtgc aggcagtgag aggtgtagat ttttatttaa acaaggggga aacattggca
     5161 attgttggtg aatcaggttc aggtaaatct gtaacaacaa aagcaattac aaaattattc
     5221 caaggggaca caggaagaat taaaaaggga gaaattttat ttttagggga agatttagca
     5281 aaaaaacctg aaaatgagtt aattaaatta cgtggcaaag atatttcaat gatctttcaa
     5341 gatccaatga catctttaaa cccaactatg caaattggta aacaagtcat ggaaccatta
     5401 attaagcaca aaaattatag taaagcacaa gctaaaaagc gcgcattgga aatactaaat
     5461 cttgtaggtt taccaaatgc agaaaaaaga tttaaagcat atccacatca attttcaggt
     5521 ggacaaaggc aaagaattgt tattgcaacc gcattagctt gtgaacctaa agtgctcatt
     5581 gctgatgaac caacgactgc attagacgta acgatgcagg cacaaatttt agatttaatg
     5641 aaagaactac aacaaaaaat cgatactgca attattttta taacgcatga tttaggggtt
     5701 gttgcgaata ttgctgatag agtggcagtt atgtatggtg gtcaaatggt tgaaacagga
     5761 gatgttaacg aaatatttta tgatccaaag catccatata catggggatt attatcgtca
     5821 atgcctgatt tatcaacaac aaatgacaca ccattactag cgattcctgg agcgccacct
     5881 gatttattac acccacctaa aggtgatgca tttgcgagac gtagtcaata tgcattagat
     5941 attgatttta aagtagaacc accgtggttt aaagtttcac cgacacattt tgtgaaatct
     6001 tggttattag acgcacgtgc accaaaagtt gaactacccg agctggtaaa acaacgtatg
     6061 aaaccgatgc ctaataatta tgaaaaacca ctcaaggtag aaagggtgtc gttcaatgaa
     6121 aaatgatgaa gtgctattat ccattaaaaa tttaaagcaa tattttaacg caggaaagaa
     6181 aaacgaagtg agagcgattg aaaatatttc gtttgatata tacaaagggg aaacattagg
     6241 tttagtagga gaatcggggt gtggtaaatc tacaactggt aaatcaatta ttaaacttaa
     6301 tgatattaca agtggagaaa ttttgtatga gggtattgat atacaaaaga ttcgtaaacg
     6361 taaagatttg cttaaattta ataaaaagat acagatgatt tttcaagacc catatgcgtc
     6421 tttaaatcct aggttaaaag taatggatat agtagctgaa ggtattgata tccatcattt
     6481 agcaactgat aaacgtgacc gtaaaaaacg tgtctatgat ttacttgaaa ctgttggatt
     6541 aagtaaagaa catgccaatc gctatcctca tgaattttca ggtggacaac gccaacgtat
     6601 tggaattgcc cgtgcattag ccgttgaacc agaatttatt atcgcggacg aaccaatatc
     6661 ggcattggat gtttcaatcc aagctcaagt agttaattta ttattaaaat tacaacgtga
     6721 aagagggatt acgttcctat ttatagctca tgatctatca atggtgaagt atatttcaga
     6781 tcgtattgca gtgatgcatt ttgggaaaat agttgaaatt ggaccggcag aagaaattta
     6841 tcaaaatcca ttacacgatt atactaagtc tttattatca gccattccac aacctgatcc
     6901 tgaatcagaa cgcagtcgca aacgatttag ttatattcat gatgaagcaa ataatcattt
     6961 aagacaatta catgaaataa gaccacagca ttttgtcttt agtactgaag aagaagcggc
     7021 acaactacga gaaaataaat tggtgacaca aaattaaggg gaagggggaa atgcaatgac
     7081 gagaaaattt agaacactta ttttaatttt gattgctaca attgcattaa gtggttgtgc
     7141 taatgatgat ggtatttatt cagataaagg tcaagtattc agaaaaattt tgtcatcaga
     7201 cttaacatcc cttgatacat cattaataac ggatgaaata tcttctgaag tgactgcgca
     7261 aacattcgaa ggtttataca cattaggaaa aggtgacaaa ccggtgttag gtgttgcgaa
     7321 agcttttcct gaaaagagta aaggtggtaa aactttaaag gttaaattaa gaagcgatgc
     7381 taaatggagc aatggtgaca aagtgactgc gcaagatttt gtttatgctt ggagaaaaac
     7441 agttgaccct aaaacaggtt ctgaatttgc atacattatg ggggacatta aaaatgcgag
     7501 tgatattagt actggtaaga aacctgtaga gcaattaggt atcaaagcat tagatgatga
     7561 aacattacaa attgatttag aaaagccggt tccatatatt aatcaattat tagcacttaa
     7621 tacctttgca cctcaaaatg aaaaagttgc caaaaaatat ggtaaaaatt acggtacggc
     7681 agctgataga gcggtataca atggtccatt taaagttgat gattggaaac aagaagataa
     7741 aacgttacta tctaaaaatc agtattattg ggataaaaag aatgtaaaat tagataaagt
     7801 gaattataaa gttattaaag acttacaagc cggtgcatca ttgtatgata ctgaatcagt
     7861 agatgacgca gttattactg cagatcaagt aaataaatat aaagataaca aaggattaaa
     7921 ctttgtgtta acgactggga cattttttgt aaaaatgaat gaaaaacaat atcctgattt
     7981 taaaaacaaa aatttaagac tggctatcgc acaagcaata gataaaaaag gatacgttga
     8041 ttcagtgaaa aacaatggct caattccttc cgatacacta acagccaaag gaattgcgaa
     8101 agcgcctaat ggcaaagatt atgcgagtac catgaattcg cctttaaaat ataatcctaa
     8161 agaagcaaga gcacactggg acaaagctaa aaaagagtta ggtaaaaatg aagtgacatt
     8221 ttcaatgaac acagaagata caccagatgc aaaaatatct gctgaatata tcaaatcgca
     8281 agttgagaaa aatttaccag gagttacttt gaaaattaag caattaccgt ttaaacaaag
     8341 agtatcacta gaactgagta acaattttga agcatcactt agtggttggt ctgcagatta
     8401 ccctgatcct atggcttatt tagaaacaat gaccacaggt agcgcacaaa ataatacaga
     8461 ctggggtaat aaagaatatg atcaattact taaagtagca agaaccaaat tggcacttca
     8521 accgaacgaa cgatatgaaa acttgaaaaa agcagaagaa atgttcctag gagatgcacc
     8581 ggtagcacca atttatcaaa aaggtgttgc acatttaaca aatcctcaag taaaaggatt
     8641 aatttaccat aaatttggtc caaataactc acttaaacat gtatatattg ataaatcgat
     8701 agataaagaa acaggtaaga agaaaaaata atatgctttg taaattaggc tggagacata
     8761 tctccagtct ttttgtgttg gataaaaact ttgggaataa aaatttaaaa taagtcgttt
     8821 tttaaattac
//
LOCUS       DAMZLE010000099         8709 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMZLE010000099 DAMZLE010000000
VERSION     DAMZLE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.100"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(86..643)
                     /gene="efp"
                     /locus_tag="POB92_002005"
     CDS             complement(86..643)
                     /gene="efp"
                     /locus_tag="POB92_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI6579478.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(669..1730)
                     /locus_tag="POB92_002006"
     CDS             complement(669..1730)
                     /locus_tag="POB92_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI6579479.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDAEEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1835..2416
                     /locus_tag="POB92_002007"
     CDS             1835..2416
                     /locus_tag="POB92_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579480.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            2430..2648
                     /locus_tag="POB92_002008"
     CDS             2430..2648
                     /locus_tag="POB92_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579481.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(2712..3542)
                     /locus_tag="POB92_002009"
     CDS             complement(2712..3542)
                     /locus_tag="POB92_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6579482.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFNTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDKHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            3700..4086
                     /locus_tag="POB92_002010"
     CDS             3700..4086
                     /locus_tag="POB92_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6579483.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            complement(4451..5923)
                     /gene="gcvPB"
                     /locus_tag="POB92_002011"
     CDS             complement(4451..5923)
                     /gene="gcvPB"
                     /locus_tag="POB92_002011"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDI6579484.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5916..7262)
                     /gene="gcvPA"
                     /locus_tag="POB92_002012"
     CDS             complement(5916..7262)
                     /gene="gcvPA"
                     /locus_tag="POB92_002012"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDI6579485.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            complement(7282..8373)
                     /gene="gcvT"
                     /locus_tag="POB92_002013"
     CDS             complement(7282..8373)
                     /gene="gcvT"
                     /locus_tag="POB92_002013"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDI6579486.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     regulatory      complement(8448..8545)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(8532..>8709)
                     /locus_tag="POB92_002014"
     CDS             complement(8532..>8709)
                     /locus_tag="POB92_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDI6579487.1"
                     /translation="DPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
BASE COUNT         2811 a         1606 c         1201 g         3091 t
ORIGIN      
        1 aggcaatagc tttatgagac ttatttagaa tatcttacgt taaatcagta tagtgaatac
       61 aagctatttg ttaacaaatt agagattatc ctcttgaaat gtagcttcca tcaccagtgt
      121 tgataattaa aacgtcacct tcgtttacaa ataaaggtac atttaatgta taaccagttt
      181 caacagttgc cgatttagta gcaccagttg cagtatcacc tttaatacca ggttctgttt
      241 cagttactgt taattcaaca gttttaggta attcaacacc gatagtttca ccttcgtatg
      301 tttgaatttg tacttccata ccttctttta agtaattcaa ttcttctttt aagtaatcac
      361 ttgaaagttc tgtttgttca aagctttcat tatccataaa tacatgatta tctccgtcag
      421 catataaata ttgcatgcga cgattttcaa tcattgctgg ttcaactttt tcaccagctc
      481 taaacgtttt ctcttgaatt gcaccagttc ttaaattacg taattttgaa cgaacgaatg
      541 ctgaaccttt accaggcttt acatgttgga agtctataac tttccaaata gcgttatcaa
      601 cagaaattgt taaacctgtt ttaaaatcat taaccgaaat cattcagttt cctcctcatt
      661 ttacacgctt atgttaaaac tataaggtct tttgtgcatt tagtaaagac ttgacaacca
      721 ttttctgtaa ttaaaatatc atcttctatt cttataccgc ccaaaccttc tatataaaca
      781 ccaggttcta ctgtaacaca gttgttaact tgaagtttat cttgtatcgt acgagccagc
      841 attggccctt catggatttc taaaccaata ccatgtccta gtgaatgtcc aaattctttt
      901 ccataccctt ttgactctaa atagtttctt gaaatggcat cagcttctgc accagtcatg
      961 ccaggtctaa tctcattaat tgctttcatt tgagattcaa gtactatttg atatatttct
     1021 ttcagtttag gatctggttc tccaatagca aatgttctag taatatctga acaatagccg
     1081 ttataatacg cgccaaaatc taatgtaatc atgtcgcctt tttcaataat tttgtcactt
     1141 gcaacaccat gtggtaatgc acctctatga ccagatgcta caatcgtatc gaatgatggt
     1201 ccatctgctc ctaattctag cattttactt tctaatattg cctttaattc tttttcagtc
     1261 atacctgctt ttacaacagt taaaatatat tcatatgttt catcaacaat attagctgct
     1321 ttttgaatta aagcaatttc ctcagcatct ttgacgtctc taattttatc tacagtatta
     1381 gaaatgctta ttaatgatat acggctttta tttaattcaa ggtatgtatc ataacttaca
     1441 tggtgcccct caaaacctac attttcaaaa ttttcttggt gtagcaattc tttaatctca
     1501 ccaataatag tagatttacg attaataatt tcataatttg gcgcttgctt agttgcttga
     1561 tcaatatatc taaagtctgt tatcaaatat tgtttatctt tagatatgat aagtgctcca
     1621 ctggtaccag taaaacctga taaatatctt ctattgtaat ccgaaagaat gataatcgca
     1681 tctaaatgtt tttgttctaa aatacgatgc acttgtgtta ttctgctcat cttattacct
     1741 cctagtgaaa cgtttgtgca tttcaacttt atacattaaa ataatatcat aatgaggata
     1801 aaaaataata gatattgatt ttagggagat agtaatgaaa aaattggttt caattgttgg
     1861 cgcaacatta ttgttagctg gatgtggatc acaaaattta gcaccattag aagaaaaaac
     1921 aacagattta agagaagata atcatcaact caaactagat attcaagaac ttaatcaaca
     1981 aattagtgat tctaaatcta aaattaaagg gcttgaaaag gataaagaaa acagtaaaaa
     2041 aactgcatct aataatacga aaattaaatt gatgaatgtt acatcaacat actacgacaa
     2101 agttgctaaa gctttgaaat cctataacga tattgagaaa gatgtaagta aaaacaaagg
     2161 cgataagaat gttcaatcga aattaaatca aatttctaat gatattcaaa gtgctcacac
     2221 ttcatacaaa gatgctatcg atggtttatc acttagtgat gatgataaaa aaacgtctaa
     2281 aaatatcgat aaattaaact ctgatttgaa tcatgcattt gatgatatta aaaatggcta
     2341 tcaaaataaa gataaaaaac aacttacaaa aggacaacaa gcgttgtcaa aattaaactt
     2401 aaatgcaaaa tcatgatagg agtcttttaa tgcgtaatat aatattttat cttgtactta
     2461 ttattgctgc gattggatta gtaatgaatc tagatgcctt tattttttca atcgtcagaa
     2521 tgttagtcag ctttgctgta atagctggta ttatttatct gatttattat ttcttcatct
     2581 taactgaaga ccaacgcaaa tatcgcaaag cgatgcgtaa gtataaaaga aatcaaagaa
     2641 gaaaatagat aaaaaaatgg aagcacttgt aggtaaaata gtctacgtgc ttccattttt
     2701 tattctaaaa actactttct aaacatccat tcatctgaac gatatttttc agttaattct
     2761 tccacttctg ccaattgagc ttctgttaat tcaagtggct ttaattctat atttaaacct
     2821 ttcttaaaac ctttctcgaa agcttcttcc atttgactaa tagtaatgtg tttatctgaa
     2881 atatcattga tggcaactgc tttttcaacg aatgcctctt tcatttttaa ttttaatctt
     2941 tcatttttat aaataaacat atcaaacagt tcatcaatat caatatcttg taaaatcgaa
     3001 ccgtgttgga ggattacgcc cttttgtctc gtttgagcac tcccagcaat cttacggcct
     3061 tcaacaacta gctcatacca acttggtgca tcaaaacaca ctgaacttcg aggttgtttt
     3121 aatttttgac gctcttcagg cgttttaggt accgcaaaat aagtattaaa tcctaagttt
     3181 ttaaatcctt ctaataatcc ttgtgaaatc actctgtacg cttctgtaac tgtagaaggc
     3241 atattcggat gcgattcagg cacaatcaca ctgtaagtta actctttatc atgtagcacc
     3301 ccacggccac cagtttgacg ccttacgaga ccaaaacctt tctctttaac cttatcaata
     3361 tcaatttctt tttgtagcct ttggaaatac cctattgata atgttgcagg attccatgtg
     3421 taaaaacgta taactggatc aatttcacct ctagagacaa aatttaataa cgcttcatcc
     3481 attgccatat tataatatgg gtctttactt cctgtattaa taaaattcca agtttcagtc
     3541 atattcaaga actcctatca gcaaaatgta taatatttga ttcgctaatt aatcaattta
     3601 actaaatgaa taataattgt aattctttag tgaaatattt tgataatttg acctaacagt
     3661 cttataatta tattatcgtt taattaggga ggatgcaaga tgagtgctag tttgtacatc
     3721 gcaataattt tagttatagc aattattgct tatatgattg ttcaacaaat tcttaacaag
     3781 cgagctgtta aagaattaga tcaaaatgaa ttccataatg ggattagaaa agctcaagtc
     3841 atcgatgtta gagagaaagt tgactatgac tacggtcaca ttaatgggtc tcgcaatatt
     3901 cctatgacaa tgttcaggca acgattccaa ggattaagaa aagaccaacc ggtatactta
     3961 tgtgatgcca atgggattgc tagctataga gccgctcgta ttttgaaaaa gaatggatat
     4021 acagatatct atatgttaaa aggcggctat aaaaaatgga ctggaaaaat aaagtctaaa
     4081 aaatagtttt tgtaaattta atatacgatt taataaaatc tgagtgttaa ttgatcatca
     4141 ataacaatac tcagatttta attttttaac aaagtctgtt actatatttc tctagcttca
     4201 ctgatcatta aacttagttt cagcataata aagaaagttc agctcatttt caatacgatt
     4261 caattaccgc aatctaaaaa atgaaaagac aatttctatg aaagaataat accaaaccct
     4321 aagagttatt acttcggttt agttttcttg tttaaataga aattgtcttt ttcaattgat
     4381 tttgaaacca ttatccttaa atcttcatac aaagttagaa taataattct cggaatatgt
     4441 gtttaatact ttatttttcc tgtttaagat tttcaaactt taatattggt ttacgagcag
     4501 ctgtagcttc gtctaatcga tcaatcacag ttgtatgtgg tgcttctagc actttatcag
     4561 gatcattttt agcttcttca gcaatactaa ttaatgtatc gataaaataa tcaagtgttt
     4621 ctttagactc tgtctcagtc ggttcaatca tcataccttc ttcaacattt aatgggaagt
     4681 atattgttgg tggatgtaca ccgaaatcta ataatcgctt agccatgtct aaagtacgta
     4741 caccaaattc tttttgacgc acaccactta acacaaactc gtgtttacaa tattgtttat
     4801 aaggtatttc aaagtgttta gataaacgtg ctttaatata attcgcatta agaaccgctg
     4861 cttcagaaac ctctttaagt ccagttgctc ccatagttcg aatatacgta taagctctta
     4921 agtaaatacc aaagttacca taaaatggtt ttacacgtcc gatagaattt ttaatgtcat
     4981 tatcatattt aaatttgtcg ccatctttaa taaccattgg ctttggtaag taacttgcta
     5041 gttcttttac tacaccgact ggacctgaac caggaccgcc accaccatgt ggaccagtaa
     5101 atgttttatg caagtttaaa tgaacagcat caaatcccat atctcctggg cgaactttgt
     5161 ccataatagc gtttaaattc gcaccatcat aatataatag acccccagca ttatggacga
     5221 tttcacggat ttccataata tttttttcga aaatacctaa agtgtttgga ttagttaaca
     5281 taatagctgc tgtattttca tttacaacac gtttcaagtc atcaatatca acttcgccac
     5341 gttcgtttga ttttacagta actgatttaa atcctgcaaa tgaagctgag gctggattcg
     5401 taccatgcgc agaatctggc acaatgactt catcacgatg accttcacca ttattctcat
     5461 gataagcttt aaatatcatc aatgcagtcc attcaccatg tgcgccagca gctggttgta
     5521 atgtcacctc atccatacca gtaatttctt ttaattcttc ttgcaaacta taaataattt
     5581 ctaatgaacc ttgaacttgg tcttcatctt gtaatggatg tgattcacta aatcctggta
     5641 ttctagcaac cttttcatta attttagggt tatacttcat cgtacatgaa cctaatggat
     5701 aaaatccgtt gtctacaccg aaatttttat ttgaaagttc agtataatga cgtactaagt
     5761 ctagttcagc aacttcagga aactccgctt tgtttttacg aataaattta tcatctaaca
     5821 atgactcaac agaatttgtt ttaatatcac tttttggtaa tgaatatgca tatctgcctt
     5881 cacgagatct ttcaaaaatt aatggacttg atttactagt catttaactc accagccttt
     5941 tctacaaatg tatcgatttc atcttttgtt cttaattcag ttacagctat taacatgtga
     6001 tttttaaagt cgtctgaaac aacacctaaa tcaaaaccac cgataatatt gtacttcact
     6061 aattcctcgt taacttgttg aattggtttg tcaaatttga ctacaaactc attgaaagat
     6121 gtaccatcta atacttcaaa acctttttta ataaattgct gtttagcata gttagcatgt
     6181 tctatatttt gaactgcaat atcatagata ccttgtttac caagtgctga cattgcaatt
     6241 gatgacgcta gagcatttaa tgcttggtta gaacaaatat tagatgtcgc tttatcacgt
     6301 cgaatatgtt gttcacgtgc ttgtaatgtt aatacaaagc cacgattacc ttcatcatct
     6361 tgtgtttgac cgactaatct acctggcact ttacgcatta actttttcgt cgttgcaaaa
     6421 tatccacaat gtggcccacc gaattgagca ggaattccga atggctgagt atcacctaca
     6481 acaatatctg caccaaatga acctggaggt gtaagtaatc ccaatgctaa tggatttgca
     6541 tatacgataa ataatgcttt tttatcttca ataaagctat gaatcttttc aagatcttca
     6601 attgaaccgt agaagtttgg atattgtact gcaacagctg ctgtttcatc atccactgct
     6661 gcttctaatt ttttcaaatc tgtaacagtg ccatctaaat cgatttccac tacttcgaat
     6721 tccttacgcg tcttagcata agtatgaagt acttgtaatg cttgataatg taaacctttt
     6781 gagactacaa ttttattttt ctttgtttga ctaaatgcta agatacatgc ttcagcaaag
     6841 ctagtcatcc catcatacat agaagaattt gctacatcca tatctgttaa ttcacaaatt
     6901 aaagtttgga actcaaaaat ggcttgtaat tcaccttgag aaatttccgg ttgatatggc
     6961 gtatatgctg tgtaaaattc tgatcttgaa atcatagcat ccacaactga tggcgcgtaa
     7021 tgatcataaa caccagcacc caaaaatgat gtatgcgttt ctttagtgat attcttgctt
     7081 gcaatgcgat ttaatcttct aagtaacgtt gtttccgctt cgccttcagc aatatttaaa
     7141 tctctattta ataaaatgtc acttggtaca tcaccgaata attctcctat agattttgca
     7201 ccaattgttt gtaacatttc ttgcttgtct ttttcagtta aaggtatata acgatgactc
     7261 acaatgcaca ccccttttta attatttatc aatttgattt ttcttaacaa ttttcgcttt
     7321 taattgacgc ttacgaactt gaacaagcaa ctctctaccc atttcaaact catctctttt
     7381 tatcattgca agtgcaattg attttcctga tgatggagac tgtgttcctg aagttacttc
     7441 tccaataata tttccatcta aatccataac ttcataacca gttcttgcaa ttcctttttc
     7501 aagtaattct aatcccactg ttcttcttgg tgcaccattt tctttttgat cttttaatac
     7561 agatttacca ataaaatcag catcaattaa tggtttactt gcaaaagcga taccaccttc
     7621 atatggtgta attgattcag ttaaatcttg tccatgtaat ggcaatccag cctctaatct
     7681 taatgtatca cgagcgccta acccacatgg cataacatta tactctaata aaccatccca
     7741 aattttttca gtatcatcga tattacaata aatttcaaac ccatcttcac ctgtgtaacc
     7801 tgactgagat aaaatgacgt ttgctccaaa taatttgaca ccctgtttaa attcaaacat
     7861 tttcatttca gttacatctt catcaactaa ttgattaatt aaatctctag cttttggtcc
     7921 ttgtattgct aattgaccat attggtttga tacattttgt acttcaacat caaatttctc
     7981 tttgtgtttt aaaatccaat taaaatcttt ttcagtatta gcagcattaa caactaataa
     8041 ataattgtcg tcagctaatt tatatattac taaatcatca ataataccgc cttcttcatt
     8101 acataaagca gtatataatg cttttgaagt agttaaatta tcagtatcat ttgataataa
     8161 atattgcaca aactgactag catctttacc tgttacttca atttcaccca tatgactaac
     8221 atcaaacagg ccaatttcgt atcgaacagc attatgctcc tctttaatac ttgaaaattg
     8281 aacaggcatc gcccatcctc cgaattccac aatttttgca cctctatcaa cataattttg
     8341 atataaaggt gtttgtttta aatcacttga cattgaaata cccccttaaa attataaaaa
     8401 caggatagca atcgctaccc tgtttataaa tcaccaattc aggaacgcgt tacagtatta
     8461 tccttttgcc tgagagtttc gtaataaatt acttgcacct tcggcgatag agtcttaacg
     8521 ctctattctc tctaatactg atcactcgct tttattaaat ttagcaattc ataatatatt
     8581 tctgaaattg atagcaaatg actatcaaat ttcttgaatg cgatttcatt atatcttaaa
     8641 ttccgcgagc aatacaagtc atttaactgc ttgattgtct tattatttgc attaggtcga
     8701 tgtgggtca
//
LOCUS       DAMZLE010000100         8576 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMZLE010000100 DAMZLE010000000
VERSION     DAMZLE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.101"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            12..893
                     /locus_tag="POB92_002015"
     CDS             12..893
                     /locus_tag="POB92_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDI6579488.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            923..1126
                     /locus_tag="POB92_002016"
     CDS             923..1126
                     /locus_tag="POB92_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDI6579489.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            1138..2235
                     /gene="ychF"
                     /locus_tag="POB92_002017"
     CDS             1138..2235
                     /gene="ychF"
                     /locus_tag="POB92_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI6579490.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(2321..2512)
                     /locus_tag="POB92_002018"
     CDS             complement(2321..2512)
                     /locus_tag="POB92_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579491.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            2756..3052
                     /locus_tag="POB92_002019"
     CDS             2756..3052
                     /locus_tag="POB92_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI6579492.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            3073..3576
                     /gene="ssb"
                     /locus_tag="POB92_002020"
     CDS             3073..3576
                     /gene="ssb"
                     /locus_tag="POB92_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6579493.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            3628..3870
                     /gene="rpsR"
                     /locus_tag="POB92_002021"
     CDS             3628..3870
                     /gene="rpsR"
                     /locus_tag="POB92_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI6579494.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(4136..5074)
                     /locus_tag="POB92_002022"
     CDS             complement(4136..5074)
                     /locus_tag="POB92_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDI6579495.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNHFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(5125..5827)
                     /locus_tag="POB92_002023"
                     /pseudo
     CDS             complement(5125..5827)
                     /locus_tag="POB92_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            6033..6644
                     /locus_tag="POB92_002024"
     CDS             6033..6644
                     /locus_tag="POB92_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6579496.1"
                     /translation="MFKKNDSKNSILLKSILSLGIIYGGTFGIYPKADASTQNSPSVQ
                     DKQFQKVEEVPNNSEKALVKKLYDRYSQNTINGKSNKSRNWVYSERPLNENQVRINLE
                     GTYRVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            7013..7414
                     /locus_tag="POB92_002025"
     CDS             7013..7414
                     /locus_tag="POB92_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6579497.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     VSYVKTTEVSTRMDDQLRSYSQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKGQVPKKSIRQIESIIACLYYMVQL"
     gene            7562..8134
                     /locus_tag="POB92_002026"
     CDS             7562..8134
                     /locus_tag="POB92_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6579498.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(8272..8535)
                     /locus_tag="POB92_002027"
     CDS             complement(8272..8535)
                     /locus_tag="POB92_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6579499.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
BASE COUNT         3058 a         1208 c         1490 g         2820 t
ORIGIN      
        1 taacgcttat catgaatcaa gtcatgaata ttatttcatc tctatttgag ccattaacaa
       61 aaatagaaac atatgaaaac attgcaacta aaatcgctat gattgttatt tatattatcg
      121 tagccctcat agttattaaa atactgaata aaatgattga acagggattt aagattcaaa
      181 ataagagtaa aaagagtaac aaaaagcgct ctaaaacttt aatatctctt gttcaaaatg
      241 tagtgaagta tatcgtttgg tttatagtta ttacgacgat tttaagtaaa tttggcatta
      301 gtgttgaagg tgttattgca agtgctggtg tcgtaggttt agcagtaggt tttggtgctc
      361 aaaccattgt taaagacgtt attactggat ttttcattat atttgaaagt caatttgatg
      421 taggtgatta tgttaaaata aacaatggtg gtacgactgt ggcagaggga acggttaaat
      481 caataggact tcgttcaaca cgaatcaata caatttcagg agaattaaca attttaccaa
      541 atagtagtat gggtgaaata acgaactact caattacaaa tggtacagct atcgttaaaa
      601 ttccagtgtc tgtcgaagaa aacattgata atgttgataa aaaactaaac aaactattta
      661 cttctttacg tagtaaatat tacttatttg ttagtgatcc ggttgttatt ggtattgatg
      721 ctattgaaga tacaagagta atattgagaa tatctgcaga aacaattcca ggtgaaggat
      781 ttgctggagc tcgaattatt cgcaaagaag tacaaaaaat gtttttacaa gaaggtatta
      841 aaacacctca accaattatg actgcttata atcatagtga aaacggtgtt tagtagttta
      901 taatacatgg aggtcatatt taatggcgtc aaaatatgga ataaatgata tagtagaaat
      961 gaaaaaacaa catgcgtgtg gaacaaaccg ttttaagatt attagaatgg gtgcagacat
     1021 aagaattaaa tgtgaaaatt gtcaaagaag tattatgatt ccacgtcaaa cgtttgataa
     1081 aaaacttaaa aaaatcatcg aatctcatga tgatacacaa agataggaga atgattaatg
     1141 gctttaacag caggtatcgt tggattgcca aacgttggta aatcaacatt atttaatgca
     1201 ataacaaaag caggtgcttt agcagcgaac tatccattcg ctacgattga tcctaatgta
     1261 gggatagtag aagtgccaga tgctagatta cttaaattag aagaaatggt tcaacctaaa
     1321 aagacattgc cgactacatt tgaatttaca gatatcgctg gtattgtgaa aggtgcttca
     1381 aagggagaag ggttaggtaa taaattctta tcacatatta gagaagtaga tgcgatttgt
     1441 caggtcgttc gtgcatttga tgatgataac gtaactcatg ttgctggtcg agtagaccct
     1501 attgatgata ttgaagttat taatatggaa ttagtactag cggacttaga atctgttgag
     1561 aaacgtttgc ctagaattga aaaattagca cgtcaaaaag ataagactgc tgaaatggaa
     1621 gtacgtattt taacaactat taaagaagct ttagaaaatg gtaaacccgc tcgtagtatt
     1681 gactttaatg aagaagatca aaaatgggtg aatcaagcgc aattactgac ttctaaaaaa
     1741 atgctttata tcgctaatgt tggtgaagat gaaattggtg atgatgataa tgataaagta
     1801 aaagcgattc gtgaatatgc agcgcaagaa gactctgaag tgattgttat tagtgcaaaa
     1861 attgaagaag aaattgctac attagatgat gaagataaag aaatgttctt agaagattta
     1921 ggtatcgaag aaccaggatt agatcgatta attagaacaa cttatgaatt attaggatta
     1981 tcaacatatt ttactgctgg tgtgcaagaa gtacgtgctt ggacatttaa acaaggtatg
     2041 actgcacctc aatgtgctgg tatcattcat actgattttg aacgtggatt tatccgtgcc
     2101 gaagtaacaa gttatgacga ctatgtacaa tatggtggcg aaagtggcgc taaagaagcg
     2161 ggcagacaac gattagaagg taaagaatat attatgcaag atggtgatat cgttcatttc
     2221 agatttaatg tataaacgat agagtgaagt taattaaata gtatatatgg agaagaggcg
     2281 gaatcaattg ttcgcctctt ttaattatgc gtataattta ttaaaagaat ggaataattt
     2341 tactcgcgtt aataatatct tgagtgctga aaaattgttt gccttcgcca gtataagcag
     2401 gctctaaaac aagattagcc tttgcacaat aaagccattc aggatgaatg ccactattaa
     2461 gtatctcttg gaattcttga aaatctttag accaatcaat atttaaattc attccttacc
     2521 acctcacaac tattatagaa cacgtgttcg cttttgtgaa gtgtattttt aaaaatatca
     2581 tagaaaattt caaatgaatt aatgtcaaaa atgatatgaa gagtagtttg aattattatt
     2641 gtaaaaagta atggcgcatg atataattct ttattgtgag taatgaaaat tattccttgc
     2701 ttatctgttt taagattgat aagccgtata gaccacaagg aggtgcaaat ataaaatgag
     2761 aacatatgaa gttatgtaca tcgtacgccc aaacattgag gaagatgcta aaaaagcgtt
     2821 agttgaacgt ttcaacggca tcttagctac tgaaggtgca gaagttttag aagcaaaaga
     2881 ctggggtaaa cgtcgcctag cttatgaaat caatgatttc aaagatggct tctacaacat
     2941 cgtacgtgtt aaatctgata acaacaaagc tactgacgaa ttccaacgtc tagctaaaat
     3001 cagtgacgat atcattcgtt acatggttat tcgtgaagac gaagacaagt aataattaga
     3061 gggggcgttt aaatgctaaa tagagttgta ttagtaggtc gtttaacgaa agatccggaa
     3121 tacagaacca ctccctcagg tgtgagtgta gcgacattca ctcttgcagt aaatcgtacg
     3181 ttcacgaatg ctcaagggga gcgcgaagca gattttatta actgtgttgt ttttagaaga
     3241 caagcagata atgtaaataa ctatttatct aaaggtagtt tagctggtgt agatggtcgc
     3301 ttacaatccc gtaattatga aaatcaagaa ggtcgtcgtg tgtttgttac tgaagttgtg
     3361 tgtgatagtg ttcaattcct tgaacctaaa aatgcgcaac aaaatggtgg ccaacgtcaa
     3421 caaaatgaat tccaagatta cggtcaagga ttcggtggtc aacaatcagg acaaaacaat
     3481 tcgtacaata attcatcaaa cacgaaacaa tctgataatc catttgcaaa tgcaaacgga
     3541 ccgattgata taagtgatga tgacttacca ttctaataaa aattaacgaa attaaagcga
     3601 aaaaattatc aaaggaggca cacaatcatg gcaggtggac caagaagagg cggacgtcgt
     3661 cgtaaaaaag tatgctattt cacagcaaat ggtattacac atatcgacta caaagacact
     3721 gaattattaa aacgttttat ctcagaacgc ggtaaaattt taccacgtcg tgtaactggt
     3781 acttcagcta aatatcaacg tatgttgact acagctatca aacgttctcg tcatatggca
     3841 ttattaccat atgttaaaga agaacaataa tatataattt attgtcaaac cccgtaggca
     3901 taggcttacg gggctttttg tgttttgggg tatagaaaaa gggcaaaaaa ggatgatgtg
     3961 aatgttttgt gttcggaatt tgcacaaaga tatgtttata ttgcaaaaat aatatgaatt
     4021 tagatgcata aaaaaagaac tacgcatttt aaataaaatg catagctctt ctttttcttg
     4081 catacgaatt aaaataactc gcgagaccta taagtctctt tcctcactag atagtttata
     4141 cttttggtct gttgaagtca ataattttat ctaaagctat aaaaaatctt ttgatagcta
     4201 atgcattatt ataatagctt tcgtttcttt tatatcgctt ttgaagttgg tccaaatcgt
     4261 gatatcttgc ttggataatt gcattactac aaacttgatt atgtaattct agcgtagcga
     4321 aagtatctat gaaatttttt attccgaaca tgtttctaga tatgcctata ttattccctt
     4381 tttcaaataa atattgagga gatctactgt cataatttag atttgctatg atgggttggt
     4441 tatgagctga tttgtttctt atatttttaa ctaaaggcat taaaatatta gcaactctca
     4501 attcttcgtc attgtacttc ttgtaataga agttgagaaa cgaaacgaat tgacctagtt
     4561 gcatgaattc aatgcaaacc catgcgggtg gattttgata gtatttattc aacttctcgg
     4621 gtagttgtcc tcgtttattc atatgcttga atatttcgtt tttatttttg attttggttt
     4681 ccataacttc ttctggtgtt cttgaatttg tgtcaaaatt tgaattgcta tatgatttat
     4741 caatacataa gaactcatct attattttat aaccatcttc ttggttattt tctgttatta
     4801 gttttaagac tagacacttt aaactatgtt caatatctaa agttaaatgc aacattgtgt
     4861 atcttaattt catatctata gttgctaaat ctgataaata agcaaattct atgaaatagc
     4921 cgccattctt tttttcgaaa ttttttcgga aataagctag tttgaagaag tgattatttt
     4981 ttctaagaat ttcatttgct ttttcggtgt caataatatt aaaaaatata ttcatctgtt
     5041 ttaatttcgc tatttgctca tcaaaattga gcataggctt aatttctgct agtgtatcat
     5101 ctttttccaa ttttaactcc ccaatcattc aaatttattc atcatatctt tcgccatctg
     5161 attagtaaca tgtgtgtata tctctagatt tttttataat ctgaatgacc tacatgctct
     5221 tgcattgctt ttaagttaat tcctaattga gcaagtgtag atatatgcga atgatgtaat
     5281 gtatgcgtcg ttacaggttt cttaatagaa ctaatctcag ttgtctcttt gatagtgccc
     5341 cctttaataa tgttgctaat tttgttcgag tcgatagggc caccagctgc atttgtgaat
     5401 atgtacccta tattaataaa cttatcatta caagcataag tgttcttagt aagtcgatgc
     5461 tatggatagt gaggcctata gccctatagc tgttacttgt tctagttgtc tcttttactc
     5521 cggatgctcc cgtcattttt cagttatcca attaacttta ccgtcgatat ctagcgtttt
     5581 atcttcatag tttatattta ctctctttat tgcaagtagc tcaccgatac gcatgtcatt
     5641 agcaatttga aactgtacca tagcttttac cattttataa ttacgttttg tcgttggata
     5701 tttttatact taattacata gtcgaaacaa tccagtaact ccttatcttc attatcttct
     5761 aaagtgttat tacgtttagc tagtaacgca tcactatagg gatatctatt ttatctatta
     5821 cacatataac tttgaattgc ttactatttt aaattaacaa attctattct cttagattta
     5881 gtccaattat gtgtagacga tttatagtta ttaaattcag agtggtagca aattaaagtt
     5941 aatcaagagt taagatgaat ttaattcatg agcacgtcta ttatttttat aattgaagaa
     6001 aataaagttt tacttcaagg aggtaattaa atatgttcaa aaaaaatgac tcgaaaaatt
     6061 caattctatt aaaatctatt ctatcgctag gtatcatcta tgggggaaca tttggaatat
     6121 atccaaaagc agacgcgtca acacaaaatt ccccaagtgt acaagataaa caattccaaa
     6181 aagttgaaga agtaccaaat aattcagaaa aagctttggt taaaaaactt tacgatagat
     6241 acagccaaaa tacaataaac ggaaaatcta ataaatctag gaattgggtt tattcagaga
     6301 gacctttaaa tgaaaaccaa gttcgtataa atttagaagg aacatacaga gttgctgata
     6361 gagtatatac acctaagaga aatattactc ttaataaaga agttgtcact ttaaaggaat
     6421 tggatcatat cataagattt gctcatattt cttatggctt atatatggga gaacatttgc
     6481 ctaaaggtaa catcgtcata aatacaaaag atggcggtaa atatacatta gagtcgcata
     6541 aagagctaca aaaagatagg gaaaatgtaa aaattaatac agccgatata aaaaatgtaa
     6601 ctttcaaact tgtgaaaagt gttaatgaca ttgaacaagt ttgaaattaa gttgaattag
     6661 tatatacagt gttttattgc taatactttg aaagttaggt atctaaaggt gcctaacttt
     6721 ctttgttatg attagcacca tcatatagaa aattctttaa tgacgtttat gccgaaatct
     6781 acaaaaataa tttctctttt atggttaatc aatatgtttt tacattcgac aaccttaaaa
     6841 ttaacttctt tcaattccat aataaagtct ctataaaata acttagttta aaaacgattc
     6901 atatctttca gattcaaata ccatcatttt ctactaatac ttacacttta attacaatta
     6961 tgtaagttgt tttcagagac atcctatttt atattttgtc agaggtgatc ttttgaaaac
     7021 gattttaaaa acaataacat atctagcact tactatcatt ggcgcttatg ctgctttatt
     7081 cattttaaaa acaatagact ctcatggtat aacagatcaa tttaacccat tagtaaagga
     7141 agatgtttct tatgttaaaa cgactgaggt gtctactaga atggatgatc aactccgaag
     7201 ttatagtcaa agtgctttta ataaagaagg gaaagagacg caattaatgt atactgctac
     7261 atttgatgtt aaaccgcata gatacttgaa aattacacat aaaggtcatc acgtagaaac
     7321 ttttgaagaa gttgaaaagg ggcaagtacc taaaaaaagc attagacaaa ttgagtcgat
     7381 tatagcgtgc ttatattaca tggttcaatt ataaaatgag tacgaacgaa agtaacacat
     7441 gatgattaat ttaaaaatat ttgtaataat tatgaataaa attaaaaaca aggggtaata
     7501 caatctatat agcatataag cttttgttat gagtttcaaa aaaggaagag agagtgatat
     7561 tatgaaatta aaatcattag cagtgttatc aatgtcagcg gtggtgctta ctgcatgtgg
     7621 caatgatact ccaaaagatg aaacaaaatc aacagagtca aatactaatc aagacactaa
     7681 tacaacaaaa gatgttattg cattaaaaga tgttaaaaca agcccagaag atgctgtgaa
     7741 aaaagctgaa gaaacctaca aaggccaaaa gttgaaagga atttcatttg aaaattctaa
     7801 tggtgaatgg gcttataaag tgacgcaaca aaaatctggt gaagagtcag aagtacttgt
     7861 tgctgataaa aataaaaaag tgattaacaa aaagactgaa aaagaagata cagtgaatga
     7921 aaatgataac tttaaatata gcgatgctat agattacaaa aaagccatta aagaaggaca
     7981 aaaagaattt gatggtgata ttaaagaatg gtcacttgaa aaagatgatg gcaaacttgt
     8041 ttacaatatc gatttgaaaa aaggtaataa aaaacaagaa gttactgttg atgctaagaa
     8101 cggtaaagta ttaaagagtg agcaagatca ctaaaaagat tcgttgcatt aacgcttgta
     8161 aaatgtctta attcatatta attattaaaa taccctcaca acgcaatgta atatatccag
     8221 tatagatttg aaaggatata tgcctaacgt gttgtgaggg tttatttgca tctatttatc
     8281 catgcgaata tcgacttctt ctaaatgttt ctgatattct ttaaccttac tttctaaaaa
     8341 catttcatat ggtgcatcaa agaaatcagc taaatgcatg gcgtctttaa atttaggttc
     8401 atggtgatga ttctcccatt cccaaatttg atgtgcttca tatttagtac catatttctc
     8461 atttaattgt tgtgctaatt cgtcaatttc taaattatgt ttagttcgta agttatataa
     8521 aatatgcata ttcattgttc attaacctcg cttcttgatt taaaattcaa agatgc
//
LOCUS       DAMZLE010000101         8265 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMZLE010000101 DAMZLE010000000
VERSION     DAMZLE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.102"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..6631
                     /gene="sasC"
                     /locus_tag="POB92_002028"
     CDS             71..6631
                     /gene="sasC"
                     /locus_tag="POB92_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDI6579500.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVASNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEPASNRPKKRSRRAAPADPNATPADPGAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNSIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVLSHAMVRTSMFEAGSNRTYQAQGNVLALGRISGTDASNHGDFNGIEKSLTVN
                     PNSELIFEFNTMPTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNSGASLDTDEFVYKIQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTTNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKKRAQT
                     ILAENRNNVPLNKRVSQADIDTLTNQMQHTLIRSVDAENAVNQKADQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKGNIIGDIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDAINQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGSVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNAEVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNVVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAFNQ
                     INQNQTNDQVDTTTNQALNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVAPEHIVRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNKKLALQNI
                     NQAVTNNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVISRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSDRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEAMKVSPKVIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEEMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6748..8016)
                     /locus_tag="POB92_002029"
     CDS             complement(6748..8016)
                     /locus_tag="POB92_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6579501.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
BASE COUNT         3246 a         1406 c         1473 g         2140 t
ORIGIN      
        1 gagggagcgt atatttttag cataattctt aacaacacag cagagaacag acaaccagga
       61 ggaaaatgaa atgaatttgt taaagaaaaa taaatatagt attaggaagt ataaagtagg
      121 catattctct actttaatcg gaacagtttt attactttca aacccaaatg gtgcacaagc
      181 cttaactacg gataataatg tacaaagcga tactaatcaa gcaacacctg taaattcaca
      241 agataaagat gttgctaata atagaggttt agcaaatagt gcgcagaata cacctaatca
      301 atctgcaaca accaatcaag caacgaatca agcattggtt aatcataata atggtagtat
      361 agtaaatcaa gctacgccaa catcagtgca atcaagtacg ccttcagcac aaaacaataa
      421 tcatacagat ggcaatacaa cagcaactga gacagtgtca aacgctaata ataatgatgt
      481 agcgtcgaat aataccacat taaatgtacc aaataaaaca aatgaaaatg gttcaggagg
      541 acatctaact ttaaaggaaa ttcaagaaga tgttcgtcat tcttcagata aaccagagct
      601 agttgcaatt gctgaaccag catctaatag accgaaaaag agaagtagac gtgcggcacc
      661 ggcagatcct aatgcaactc cagcagatcc aggggctgca gcggcaggaa acggtggtgc
      721 accagttgca attacagcgc catacacgcc tacaactgat cctaatgcca ataatgcagg
      781 acaaaatgca cctaacgaag tgctgtcatt tgatgacaat agtattagac caagtaccaa
      841 ccgttctgta ccatcagtaa cagttgttga taatttacca ggctttacac ttatcaatgg
      901 tggtaaagta ggtgtgctaa gtcatgcgat ggttagaaca agtatgtttg aggctgggag
      961 caacagaacc tatcaggcac aaggcaatgt acttgcatta ggacgtataa gcgggacgga
     1021 cgctagtaat catggagatt ttaatggtat tgaaaaatct ttaacagtaa atccaaattc
     1081 tgaattaatc tttgaattta atacaatgcc aactaaaaat ggtcaaggcg caacaaatgt
     1141 tattatcaaa aatgctgata ctaatgatac gattgctgaa aagactgttg aaggcggtcc
     1201 aactttgcgt ttatttaaag tacctgataa tgtgagaaat ctcaaaattc aatttgtacc
     1261 taaaaatgac gcaataacag atgcgcgtgg catttatcaa ctaaaagatg gttacaaata
     1321 ctatagcttt gttgactcta tcggacttca ttctgggtca catgtttatg ttgaaagacg
     1381 aacaatggaa cccacagcaa cgaataataa agaatttaca gttacaacat cactaaaaaa
     1441 taatggtaat tctggtgctt ctttagacac agatgaattt gtttataaaa ttcaattacc
     1501 tgaaggggta gaatatgtga acaattcatt gactaaagat tttccaagta acaattcagg
     1561 cgttgatgtt aatgatatga atgttacata tgatgcggcg aatcgagtga taacaattaa
     1621 aagcactggt ggtggcacaa cgaattcacc tgcacgacta atgcctgata aaatattgga
     1681 tttaaagtat aagttgcgtg ttaataatgt accgacacca agaacagtaa catttaatga
     1741 tacattaacg tataaaacat atacacaaga tttcattaat tcagctgcag aaagtcatac
     1801 tgtaagtaca aatccatata ctatcgatat catcatgaat aaagatgcat tacaagccga
     1861 agttgacaga cgtattcaac aagctgatta tacatttgca tcattagata tctttaatga
     1921 tcttaaaaaa cgtgcacaaa caattttagc tgaaaatcgt aacaatgtac cattaaataa
     1981 aagagtatct caagcagata ttgatacatt aactaatcag atgcaacata ctttaattcg
     2041 aagtgttgat gctgaaaatg cagtaaatca aaaagctgat caaatggaag atttagttaa
     2101 tcaaaatgat gaactgacag atgaagaaaa acaagcagca atacaagtta tcgaggaaca
     2161 taaaggcaat attattggtg atattggtga tcaaacgact gatgatggcg ttactagaat
     2221 caaagatcaa ggtatacaga ccttaagtgg ggatactgca acaccggttg ttaaaccaaa
     2281 tgctaaaaaa gcaatacgtg ataaagcaac gaaacaaagg gaaattatca atgcaacacc
     2341 agatgctact gaagacgaga ttcaagatgc aataaatcaa ttagctacgg atgaaacaga
     2401 tgctattgat aatgttacga atgctactac aaatgctgac gttgaaacag ctaaaaataa
     2461 tggcatcaat actattggat cagttgttcc tcaagtgaca cataaacaag ctgcaagaga
     2521 tgcaattaac caagcaacag caacgaaaag acaacaaata aatagtaata gagaagcaac
     2581 tcaggaagag aaaaatgcag cattgaacga attaactcaa gcaaccaacc atgctttaga
     2641 acaaatcaat caagcaacaa caaatgctga tgttgataac gccaaaggag atggtctaaa
     2701 tgccattaat ccaattgctc ctgtaactgt tgttaagcaa gctgcaaggg atgccgtatc
     2761 acatgatgca caacaacata tcgcagagat caatgctaat cctgatgcga ctcaagaaga
     2821 aagacaagca gcaattgaca aagtgaatgc tgctgtaact gcagcaaaca caaacatttt
     2881 aaacgctaat accaatgctg aagtggaaca agtgaagaca aatgcgatac aaggaataca
     2941 agcaattaca ccagctacaa aggtaaaaac agatgcaaaa aatgccatcg ataaaagtgc
     3001 ggaaacgcaa cataatacga tatttaataa taatgatgcg acgctcgaag aacaacaagc
     3061 agcacaacaa ttacttgatc aagctgtagc cacagcgaag caaaatatta atgcagcaga
     3121 tacgaatcaa gaagttgcac aagcaaaaga tcagggcaca caaaatatag tagtgattca
     3181 accggcaaca caagttaaaa cggatgctcg caatgttgta aatgataaag cgcgagaggc
     3241 gataacaaat atcaatgcta cacctggcgc gactcgagaa gagaaacaag aagcgataaa
     3301 tcgtgtcaat acacttaaaa atagagcatt aactgatatt ggtgtgacat ctactactgc
     3361 gatggtcaat agtattagag acgatgcagt caatcaaatc ggcgcagttc aaccgcatgt
     3421 aacgaagaaa caaactgcta caggtgtatt aaatgattta gcaactgcta aaaagcaaga
     3481 aattaatcaa aacacaaatg caacaactga agaaaagcaa gtggctttaa atcaagtgga
     3541 tcaagagtta gcaacggcaa ttaataatat aaatcaagct gatacaaatg cggaagtaga
     3601 tcaagcgcaa caattaggta caaaagcaat taatgcgatt cagccaaata ttgttaaaaa
     3661 acctgcagca ttagcacaaa tcaatcagca ttataatgct aaattagctg aaatcaatgc
     3721 tacaccagat gcaacgaatg atgagaaaaa tgctgcgatc aatactttaa atcaagacag
     3781 acaacaagct attgaaagta ttaaacaagc taacacaaat gcagaagtag accaagctgc
     3841 gacagtagca gagaataata tcgatgctgt tcaagttgac gtagtaaaaa aacaagcagc
     3901 gcgagataaa atcactgctg aagtggcgaa gcgtattgaa gcggttaaac aaacacctaa
     3961 tgcaactgac gaagaaaagc aggctgctgt taatcaaatc aatcaactta aagatcaagc
     4021 atttaatcaa attaatcaaa accaaacaaa tgatcaggta gacacaacta caaatcaagc
     4081 gttaaatgct atagataatg ttgaagctga agtagtaatt aaaccaaagg caattgcaga
     4141 tattgaaaaa gctgttaaag aaaagcaaca gcaaattgat aatagtcttg attcaacaga
     4201 taatgagaaa gaagttgctt cacaagcatt agctaaagaa aaagaaaaag cacttgcagc
     4261 tattgaccaa gctcaaacga atagtcaggt gaatcaagca gcaacaaatg gtgtatcagc
     4321 gattaaaatt attcaacctg aaacaaaagt taaaccagct gcacgtgaaa aaatcaatca
     4381 aaaagcgaat gaattacgtg ctaagattaa tcaggataaa gaagcaacag cagaagaaag
     4441 acaagtagca ctagataaaa tcaatgaatt tgtaaatcaa gccatgacag atattacgaa
     4501 taatagaaca aatcaacaag ttgatgatac aacaagtcaa gcgcttgata gcattgcttt
     4561 agtagcgcct gaacatattg ttagagcagc tgctagagat gcagtaaagc aacaatatga
     4621 ggctaaaaag caagaaatag agcaagctga acatgcgact gatgaagaaa aacaagttgc
     4681 tttaaatcaa ttagcgaata ataaaaaact tgcattacaa aacatcaatc aagcagtaac
     4741 gaataatgat gtgaaacgtg ttgaaacaaa tggcattgct acactaaaag gtgtacaacc
     4801 tcatattgta attaagcctg aagcacaaca agcaataaaa gcaagtgcag aaaatcaagt
     4861 agaatcaata aaagatacac cacatgcaac agttgatgaa ttagatgaag cgaatcaatt
     4921 aattagcgac acactcaaac aagcgcaaca agaaatagaa aatacaaatc aagatgctgc
     4981 tgttactgat gttagaaatc aaacaatcaa ggcaattgag caaataaaac ctaaagtaag
     5041 acgtaaacga gctgcgcttg atagcattga agaaaataat aaaaatcaac tcgatgcaat
     5101 ccgaaatacg ttggatacta ctcaagatga aagagatgtt gctattgata ctttaaataa
     5161 aattgtaaat acaattaaaa atgacattgc acaaaacaaa acgaatgcag aagtggatcg
     5221 aactgagact gatggcaacg acaacatcaa agtgatttta cctaaagttc aagttaaacc
     5281 agcagcgcgt caatctgttg gtgtaaaagc cgaagctcaa aatgcactaa tcgatcaaag
     5341 cgatttatca actgaagaag aaagactagc tgctaaacat ttagtagaac aagcacttaa
     5401 tcaggctatt gatcagatca atcatgcaga taagactgcc caagttaatc aagatagtat
     5461 agatgctcaa aatattattt caaaaattaa accagcgaca acagttaaag caacagcatt
     5521 acaacaaatt caaaatatcg ctacaaataa aattaattta attaaagcaa ataacgaagc
     5581 gacagatgaa gaacaaaata ttgcaatagc acaagttgaa aaagagttaa ttaaagctaa
     5641 acaacaaatt gctagtgcag tgactaatgc tgatgtggca tatttattgc atgatgagaa
     5701 aaacgaaatt cgtgaaatcg aacctgttat tagcagaaag gcgtctgctc gagaacaatt
     5761 gacaacatta ttcaacgata aaaaacaagc aattgaagcg aatattcaag caacggtaga
     5821 agaaagaaat agtatattag cacagttaca aaatatttat gacactgcta ttggacaaat
     5881 tgatcaagat cgtagcaatg cacaagttga taaaacagca tcattaaatc tacaaacaat
     5941 acatgattta gatgtacatc ctattaaaaa gccagatgct gaaaaaacga ttaatgatga
     6001 tcttgcacgc gtcactgctt tagtgcaaaa ttatcgaaaa gtaagtgatc gtaataaggc
     6061 tgatgcatta aaagctataa ctgctttaaa attacaaatg gatgaagaat taaaaacagc
     6121 acgcactaat gctgatgttg atgcagtttt aaaacgattt aatgttgcat taagcgatat
     6181 agaagcagta attactgaaa aagaaaatag cttactgcgc attgataaca ttgctcaaca
     6241 aacatatgcg aaattcaaag cgatcgcaac accagaacaa ttagctaaag taaaagtatt
     6301 aattgatcaa tatgttgcag atggcaatag aatgattgat gaagatgcga cattaaatga
     6361 catcaaacaa catacgcaat tcattgttga tgaaatttta gcaattaaat taccagctga
     6421 agcgatgaaa gtatcaccaa aagtaattca gccagctcca aaagtttgta cgcctattaa
     6481 aaaagaagag acacatgaat cgcgcaaagt tgaaaaagaa cttccaaata caggttctga
     6541 agaaatggat ttaccattga aagaatttgc actgattaca ggtgcggctt tgttagctag
     6601 aagacgtact aaaaacgaaa aagaatcata attaacgaaa tgaccttaat ataacgacac
     6661 tttttagtga ctcagttata ttaaggtcat ttttaattga atgaaaaagt tgatctacta
     6721 aagagaatcg aaccaaattt tatactatta ttccattgat gcatgtgagt aatgtccggc
     6781 atataatcca gcgacatgtc ctgttacgag tgcacttgta atattataac caccagtata
     6841 accatgtata tctaatactt caccacataa aaataatccc ggaactaatt tagacatcat
     6901 tgttttaggt tgaatttctt taagtgacac accaccacct gtgacaaatg ccttatctat
     6961 aggtaatgtc ccgttcacct taaatacaaa ccctttaaac atatttacta agtcgttcaa
     7021 ttgttgattt gataagtgat gtgatgtggt attttcatcg attcctgctt gttccagcat
     7081 gaacagtaag tagcgctctt caattagacc atgcaaactg tttttaatga ttttatctgg
     7141 tgtgtccgat aataatgatg tgatgtgttg ttttaattgt tcatggttta attcaggaaa
     7201 tgcatcgatt gccatagaaa tgtgctgtgt cttttgattt ttttgttctt tataaacaaa
     7261 ctgactacat cttaatgcag ctggaccact gataccaaaa tgagtaaata acatatccat
     7321 ttgatgactg atgcgttttt taccattttt cttaagtact gacaattcaa catcttttaa
     7381 acttaaacct tttagacgat tggatttgat gaaaggttca gctgatgtaa ttggaacttc
     7441 ggtcgggaat aactcagtaa tggtatgacc taaatcttgt gcgaacttat aaccatcacc
     7501 agttgaacca gtttgaggga cacttgtacc acctgtagca atcactagcg aatggctttc
     7561 ataactatta ttttgagtat gtacagtgaa agtttggtct gtattaactt cgattctact
     7621 aacagcttct tcttctttaa tcgttacatg ttggcgttcg atagttgtca ctaatgtatc
     7681 aaccacgtct tgtgctttgt tggaaactgg aaacatacgc ccgtgatctt cttcttttaa
     7741 tttaacaccc ctagactcaa aaaaatctat gatggattca ttatcaaaaa ttgaaaaggg
     7801 actatataaa aatttcccat ttccaggaat gttcttaata atttcagcat atggtaatcg
     7861 attagttacg ttacatctac cgccaccaga tattttgagt ttacgaccta gacctttctt
     7921 tttttcaatg agtaacacac tgctactttg ttcgcttgca gctactgccg ccattaagcc
     7981 gctaggtccg cctccgataa taattgtttg atacatatac gcttcctcca aatttgttac
     8041 tggacattca attgctattt tatagtactt ttttaaactt tgctatatct cttgtgttat
     8101 gagatgacaa ttatgttgaa tatatttata atgtgaactt cataaaaaat gaagatgtat
     8161 gtgtatgata agaatgattt ttctactgtt atgcgatgtt catctcaaat agtagtataa
     8221 tgtttatatg aaatttcgta atattctaat caaaatctag tattc
//
LOCUS       DAMZLE010000102         7935 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMZLE010000102 DAMZLE010000000
VERSION     DAMZLE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.103"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(244..936)
                     /locus_tag="POB92_002030"
     CDS             complement(244..936)
                     /locus_tag="POB92_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6579502.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFAGVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1249..2193
                     /locus_tag="POB92_002031"
     CDS             1249..2193
                     /locus_tag="POB92_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579503.1"
                     /translation="MRKIMRILLCASIIVSGCSFIPKNQTKSIPKTVSVKDYDGKYIG
                     EHKKRNEVFLKKHKDEAIKKYKDYVKDTFGYDCKINLVDAYTNKSGFSEKSKVDGISV
                     VGTVNYDVPFQLQLLFVESGNGITISTFTPGHINETSAAVSAMMYKRYEHEIEQARLK
                     LKSEIEKDSYYAMSEKLQVKQEFNGVTRQYLNFRTDSIDDLDKFKKEFKPVMHLKGDA
                     FNQQLQSLINKYPQIQKNMKSEFIAYYDKEKNRETVKNYAWNLQKSINDIMQSYPSTK
                     FVQFYKDDVSPSMVDGNGRLKSDTNVISIEGGKYDENK"
     gene            2180..2482
                     /locus_tag="POB92_002032"
     CDS             2180..2482
                     /locus_tag="POB92_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579504.1"
                     /translation="MRTNNHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINAMSQNEGKSVDALKNSTAPLKNNLTKLKEQLDKTTH"
     gene            2636..2995
                     /locus_tag="POB92_002033"
     CDS             2636..2995
                     /locus_tag="POB92_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579505.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(3080..3691)
                     /locus_tag="POB92_002034"
     CDS             complement(3080..3691)
                     /locus_tag="POB92_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6579506.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3770..4246)
                     /locus_tag="POB92_002035"
     CDS             complement(3770..4246)
                     /locus_tag="POB92_002035"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDI6579507.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(4249..4749)
                     /locus_tag="POB92_002036"
     CDS             complement(4249..4749)
                     /locus_tag="POB92_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579508.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4984..5751)
                     /locus_tag="POB92_002037"
     CDS             complement(4984..5751)
                     /locus_tag="POB92_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDI6579509.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5748..6440)
                     /locus_tag="POB92_002038"
     CDS             complement(5748..6440)
                     /locus_tag="POB92_002038"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDI6579510.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(6457..7119)
                     /locus_tag="POB92_002039"
     CDS             complement(6457..7119)
                     /locus_tag="POB92_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDI6579511.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2678 a         1399 c         1100 g         2758 t
ORIGIN      
        1 agttaattta caactaaacg ataaatatta tatgcaaaat acatctttaa tattaaagta
       61 ataccaatat tttttcaata aacctagtgt aatatatgtg taattctaaa agattcttct
      121 ttaaaaatat aaataccacg acatattgct ttaacatttt catttataaa gcgaaaaaat
      181 gcatcgctac taagttgaat gtttagtaag gatgcattga attcactaaa atgattaaat
      241 tacttatatc ttttcatctg attgattatc gaaatttctt ccttctaaac ctgctaactc
      301 ttctttagaa gctgcaggtg ctttcatttc aaatatctca ttcactactg tgtaatcgta
      361 atatcctaat ctggcaatag gtttaatcga cttaatgtcc aatttaccat tatcaagaat
      421 aaccttatcg tcaatatgaa cttgagcaac tcttcctata acaatatcta cggtagatac
      481 tggatctcca gttggaatac gaatcgtttg aacgtactca cattcaaaat gaactggcga
      541 ttcttttaca cgatatcctg gagcttctat acatttttcc tttgttacac ctgcaaaatt
      601 aaattcatcc tcttctggtg gcaatgcttt cgatgataaa ttaactgctt ctcttaaatc
      661 atacgttgcc atattccaca caaaccaacc tgtctcttca gcatttttca ctgtatcttt
      721 acgttcgtga tcaccaagaa cggattgatt tgctgcgaac ataaccatag gcggatccca
      781 agttaagttt tgatactgac tataaggcgc taaattatct ttcccatctt tcgatacagt
      841 agagatccac cctattggac gtggtactgt actactttta aatgggtcgt gcggtaaacc
      901 atgacttctt acaccttgtt ttggcgaata attcatacta tcttcacccc ttataagtaa
      961 ttacatttaa ggttacgccc tctttacata agcgtctaat ataaataaac aatttattta
     1021 taagtagaaa ctatatatga cgtggttgct tataatttgc gttcttgatc cgaaaatttc
     1081 agataaggat ttatgcaatt aatatttatg atatcttttg taaatttaat taattatagt
     1141 tacttcaatc atgattagtt tataataata ttgaataaca attttttgat ttttgtgtaa
     1201 tttatctatc aactgaaaaa cagctaatac aaaggagcta atgaagatat gagaaaaatt
     1261 atgcgaatct tattgtgcgc atcaatcata gtaagcggtt gctctttcat accaaagaat
     1321 caaacaaaat ctattccaaa aacggttagc gtcaaagatt atgatggtaa atatattggt
     1381 gaacataaaa aaagaaacga agtatttctg aaaaaacata aagatgaagc aatcaaaaaa
     1441 tacaaagact acgtgaaaga cacctttggt tatgattgta aaattaattt agttgatgcc
     1501 tacactaata aatctggatt tagcgagaaa agtaaggtag atggtatctc agtggttggt
     1561 actgtcaatt atgacgttcc ttttcaactc caattgcttt ttgttgaatc tggaaatggt
     1621 ataactattt caacatttac acctggtcat ataaatgaaa catcagcagc cgtttcagcc
     1681 atgatgtaca aacgttatga acatgaaatt gaacaagctc gattaaagct taaaagtgaa
     1741 attgaaaaag acagctatta tgcgatgagc gaaaaacttc aagtaaaaca agaatttaac
     1801 ggcgttacaa ggcaatactt gaatttcaga acagatagta tcgatgatct ggataagttc
     1861 aaaaaagaat tcaaacctgt tatgcactta aaaggtgatg cattcaatca acagttacaa
     1921 tctttgatta acaaatatcc acaaatacaa aaaaatatga aatcagagtt cattgcttat
     1981 tatgataaag aaaaaaatag agaaacagta aaaaactatg cttggaacct tcaaaaatct
     2041 ataaatgaca ttatgcaatc atatcctagc acaaaatttg tacagtttta taaagatgat
     2101 gtttccccgt caatggtaga tggaaatggc cgtttaaaat cggatactaa tgtaatttcc
     2161 atcgagggag gaaagtatga tgagaacaaa taatcatata accgatttag atgctattga
     2221 gttaagtagc ggttggaatt acaattcgaa attggagact ggcaaaaatg cactactatc
     2281 agatagcctt ttaagagata ttaaagtatt aagttctaat gctaaaggcg acgctgacat
     2341 actagaaaca gaacttcgtg ctgtaataaa cgcaatgtct caaaacgaag gtaaatctgt
     2401 tgatgcactt aaaaatagta ccgctcctct aaaaaacaat ctaaccaaat taaaagagca
     2461 actcgacaaa actacacatt aatttcatac attttgagtg aacagaatta tatttccata
     2521 gcaaacattc ctaaactaca catacgttac ataattgatt catttttata gaaacgggta
     2581 aaaatgataa agtaatcatc tttcacaaaa aatcaaatat tgggaggaat taaatatgat
     2641 tttgcgttac gcaacgaatt taaaacttgc gaaagagcta taccaagcca gtaaacctaa
     2701 attgcaagaa gataagggta tgattgaact gtttgaacat acttttggct tgcaaaaaga
     2761 acttgtaaaa tacgtcggca ttgcagaagc aactactgct gctttatact cagctagctt
     2821 tatcaataaa aacatttctc gccttgcatc actatcaacg ataggcattt taagtgtagc
     2881 tgcttacaaa catttcgaag ctggccaagg taaaaaaggt gcacaacatg cattagattt
     2941 attaggtctt gctactttaa gtttactaga tacattttct tgtaataaaa aatagtcttc
     3001 attttagaag cacatacttt agcacctgtg tacttttgat tcaatccata aacggtacac
     3061 cgctatagca tgtgttttat tatttttctg gtctctttat cgtaaatatt ccccctaatt
     3121 tagcataatc atttccggct aaacgagaaa cagcctgtaa tgcgtgtgga ttgattttaa
     3181 aatcaccctc ataaatactg tcatcaatat gataagttac aatttcacct attattaaat
     3241 cagccccatc taatacatct ccaagcaata tcatttgcga tagtttacat tcgaatctca
     3301 ttttcgcatc tttaattcct ggcgtcttaa tcgttgtaga tgttaaaagt gataattctg
     3361 tacgactcaa ctcactgtca ccatatgcta acggcgctgc agtctcatta atatcttgaa
     3421 cattatcttc gtctgtaata tgcacaacaa agtctccagt ccgttctata tttaatgcag
     3481 tatcttttct cttacctcct gcacgttgaa ctgcaatagc aatcattggc ggatgattat
     3541 taacaatatt aaaaaagcta aatggtgctg catttactga tgcatcttga tttaatgttg
     3601 taacaaaagc tataggtcgt ggaataattg aaccaattaa taatttatag ttttctctag
     3661 cagttaatga ttgtgcatca aacgtataca taatacctac ctcttttcta agtatatcta
     3721 ggtatttctc cgattttggt taatttaaac atctattctc ctctgaaaat cacttgtatt
     3781 tatttagcaa atcttttgaa atatgacaca tatgcatatc ttctggatat ttttctaaat
     3841 gttgctgatg ttcttcagca cttttaatgt agttagacag cggtaagact tccactgcaa
     3901 tttgatctct gtctttacgt cgttcaatga actgacgcgc ttcaattaag tggtcatcta
     3961 cacaactata taaacccgtt cgatactttt gtccaatatc atttccttgt tgattcacac
     4021 tgtaaggatc aatgatttca aataaataat tcataatgtc tgtaattgtt aacatacgat
     4081 catcgaaatg aagtttgaca cattcagcat aaccatcata cggaccgtct aatttagaac
     4141 ttcttccatt tgctcttcct gcttctgtat gtataattcc aggtattgtt gcaaaaaatg
     4201 cttcaacacc ccataaacat cctcctgcta cataaacaac tgccatattt acacctcatc
     4261 atcctttttt atatttttaa caaggttata ccatttaata ccgccatgac atgattctga
     4321 tacaccttca ttacgatacc catatttttc ataaaatgaa attaatgatt ctcgacatgt
     4381 taacgttaca ccatgtcgat gatgattctt agcaagagtt tcaaaatagt ttagtaagcg
     4441 acctgcaata ccctgacctt gataatttgg tgctacaaca agacctaaca cactaatata
     4501 gccaccttca ctattatttg tggagacatt tttaaataaa tcatcgctaa tgtaacgctc
     4561 ttttatgact ggaccgttga tgtatccaat tacttcatca ttcttttcag ctacaataaa
     4621 agtttcttga atttgttcaa ttctcaattt caatgcctct cgtgttgcag cttcttccgg
     4681 tgaaaaacca atattttcaa gtgcaataat ttgatctaag tcctgcatga ttacttttct
     4741 taataccata aaaacacttc cttagcccta tatatcaaaa gtaatacctt catctttata
     4801 ttcattattt tcaaactacc ttcactataa aaaacgagtc tttttattga aagccattcg
     4861 ccttaatcaa ttagacaagt tgtattatac gaattagtaa ataatcatga ttataattca
     4921 ttttaagcat actcaaaaag actggtacat gtataccagt ctaattcgaa aaaatatatt
     4981 tagttaaaac catttcaaaa acgacttcac ttttcctggc ttagtcggtc tgtctaaata
     5041 aacaaaatta tcattgatta ctgaactggc atttcgaagt aatgcttcaa tatcattcga
     5101 atatttcttc aatttatgat tgtgaaataa ttcttgcatc aaaaatggtc tttggtcaca
     5161 tgaatgtgca tctgaagcta caaaatgagc caaattacat tctataaatt gtaatgataa
     5221 cttttgaatg tttttaccaa atccaccaac taaagaactc gatgttaatt gactcaatgc
     5281 cccatttgca accaattcat ataatatttc cggatttttg gcgatacttc tatttctttc
     5341 aggatgtgca atgattggta tgtaacctct cgattgtatt tcaaaaaaca attgttttgt
     5401 ataatgtggt acttcgcccg ttggaaattc aattaataaa tatttcgaac gattaatacc
     5461 ttgaatacta ccattatcta agcctttcag aatcgaatct gtaattctaa tttcttgccc
     5521 gggaaataat ttaatatcca atgcttgaac ttctggatgc gttcttaact ccgccaattt
     5581 cacaagcact tgttgaaatg tattatcata tctcggatgc aaatgatgag gtgtcgctac
     5641 aatacttgtt acaccttcat ccttagcttg ctttaatagt gcaatactct tttcaattgt
     5701 tttaggacca tcatctatat caactaatat atggttatga atatcaatca tgattcatca
     5761 gtcccataat atgcatagta actagcactt ttatctttag gcattctatt taagactaca
     5821 cctaataatt tagcacctgt tgcttcaata agttcttttc cttttttaac ttcatcttta
     5881 ttattatttt ccgaattaac tacgtagaca acattgccgg taaactttga aaataattgc
     5941 gcatctgtaa ctgtgttcac tggtggcgta tcgataatta caaagttata attcatcaat
     6001 aatgtgtcat acaaatttgc aaatgccctt gatgtaatta actctgacgg attcggtggg
     6061 attggcccag acgtcaagac gtctaaatct tgaatttcag ttgagataat actgtcttga
     6121 taagttgacc aatttagcaa taaacttgat aggccttcat tgtttggcaa attaaaaata
     6181 taatgctgcg taggtttacg catatccccg tctacgatta gtgttttata acctgcttgc
     6241 gcatatgcaa ctgctaaatt tgctgcaatt gtagacttac ctgcgcctgg tgcctctgaa
     6301 gtgattacaa tgctttgaac tgcactgtca ggatttgcaa acataatatt tgatcttatg
     6361 cctcgaaatt tctcgctaat aggtgacttt ggttgttcat ggacaattaa acttgatgta
     6421 cttcttcgtg tattcgtcat ggtaattcct cgtaaattaa aatttttgta ttgaacctaa
     6481 aataggtaat cctagttgcg attcaacatc ttcttctgtc ttaatacgct tatctaataa
     6541 ttcttttaag aaaataatca atattgctaa aacaatacca acaataatgc tgataactaa
     6601 gttgacagat actattggag atacttttac agcattatca tgtgctgagg aaagtatcgt
     6661 aacattatca acactcataa ttttaggcat gtcatgagca aaaactttag atattttatt
     6721 aacaattttg tcagattcag atttattccc agtggtaact gatacagtaa taatttgaga
     6781 gtttgtttga ttggttactt ttaaaaatga attcaactca gctgttgaat actgaccatc
     6841 aaattctcta gatactttat ctagaattct aggacttttg ataatttccg tatatgtatt
     6901 aacagactgc aaactacttt gaacattttg gaaagctaaa tcacttgagg actttttcat
     6961 gttcactaat atttgagtag aagcagtata tttgtcaggc ataacaaaaa aggttaatgc
     7021 cgcacttact acaagacata ttgccggtaa aataagcaat aatttaatat tcttctttag
     7081 aatatttaat agttttacta aatcaaactt ttctttcatg gtttcctcca cataatcaat
     7141 cattgtattc attatgtatg ttttataaat cggacaatta tatctagttt aacgaccaca
     7201 aaacatacac aactacattt tctctaatta tttatataaa tattttatcg tttaaaatta
     7261 tatcatgatt ctctaccatt atgtataact tatttatatt tttgcacaag atataatatt
     7321 gtccaacttt aaatatccaa acctattaat aataaaacta gataccatcg tactctatca
     7381 tggctttctt ataatcgagt agaagcatca tcattacttg attatttgct ctttacaaca
     7441 ccgagcgtgc ccgtactcgg taattcaata ccttgcgtaa cccgtcactg tgagttgggt
     7501 taatgataat aaagcccaca ccttttaaaa agatgtgggt aatttatata atttttattt
     7561 acatttttaa cttataaaaa aaagcgccta tgtcatgatt taccatcaca taggcgctta
     7621 tcaataaatt attacttatt actttccatt tcatctaatt tatgcggatt tcctgtaatt
     7681 agatgacaac ttattctttt caggggaaca ttacactttt ataatatgtt caaagacaaa
     7741 cttaaccatt cacaaatata aagaataata ttatcaaatc attgaacaaa tcgtattttg
     7801 catcaattga tatttatatt aatgtattgc atttaattta taaaattcat atacatctta
     7861 atattctcaa tatcgatttg tattgtcaac tttatataga tttaaaaaaa taatctcatg
     7921 tcttttttta caaaa
//
LOCUS       DAMZLE010000103         7911 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMZLE010000103 DAMZLE010000000
VERSION     DAMZLE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.104"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            104..1246
                     /gene="bshA"
                     /locus_tag="POB92_002040"
     CDS             104..1246
                     /gene="bshA"
                     /locus_tag="POB92_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDI6579512.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYGLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            1251..2453
                     /locus_tag="POB92_002041"
     CDS             1251..2453
                     /locus_tag="POB92_002041"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDI6579513.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVIKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            2440..3411
                     /locus_tag="POB92_002042"
     CDS             2440..3411
                     /locus_tag="POB92_002042"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDI6579514.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSVQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            3435..6128
                     /locus_tag="POB92_002043"
     CDS             3435..6128
                     /locus_tag="POB92_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HDI6579515.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRSENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            6450..7742
                     /gene="asnS"
                     /locus_tag="POB92_002044"
     CDS             6450..7742
                     /gene="asnS"
                     /locus_tag="POB92_002044"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI6579516.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFINHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKSEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
BASE COUNT         2943 a         1049 c         1440 g         2479 t
ORIGIN      
        1 tatttgaaat tataaactca acattactat aaacttaaat ttaaacaatt atctacgcat
       61 cgagaataat agagagaaat agaaagaaag gatgggcaag cgaatgaaga taggtataac
      121 atgttacccg tccatgggtg gttctggaat tattgccaca gaattaggaa ttaaattagc
      181 agagcgaggt cacgaagtac attttatcac ttcaaatatc ccgtttagaa taagaaaacc
      241 attaccgaat atgatttttc atcaagttga agtgaatcaa tatgcagtat ttcagtatcc
      301 accgtatgat attacgttaa gtactaaaat cgctgaagtt attaaagaat atggtcttga
      361 tttgttgcat atgcactatg ctgtacctca tgcaatttgt gggattttag ctcgtgagat
      421 gtcaggtaaa gatataaaaa ttatgacaac gctacacggt actgatatta ctgttttggg
      481 ctatgatcat tcactccaag gtgcaattaa atttggcatt gaaaaaagtg atattgtgac
      541 aagtgtgagt aaatctttag cacaagaaac acatgaaatt attgaaacta acaaagaaat
      601 tataccgatt tataattttg tacgtgaaaa tgagtttcca actaaacata acacagcatt
      661 aaaatcacaa tttggtatag caccagatga aaaagtgtta atacatgtat cgaattttag
      721 acaagtaaaa cgcatagata ctattataga aacatttgca aaagtacgtg aaaagatacc
      781 tagtaaatta attttacttg gagatggtcc tgaattagtg ccaatgcgtc aattgacgaa
      841 agagttaaac gtagaagagg atgttttatt tttagggaaa caagattgcg taagtgaatt
      901 ttaccaatta tctgacttag tattattatt aagtgaaaaa gaaagctttg gactaacttt
      961 acttgaggct atgaaaacag gcgtcgttcc aattggttca aatgccggtg gaattaaaga
     1021 ggttattaaa catggtgaaa ctggatttgt cgtagatgtg ggtgattgtg attcagcgag
     1081 tgactatgct atccgattac ttgaagataa ggtgttatac aacaaacttc agaaaaacat
     1141 gctagcagat attgctgaac gttttggatc agaacttatt acagatcaat atgagtatta
     1201 ttatcaaaaa atgctaaatg agcataacaa aagtaaaggc gagtaataat atggataaat
     1261 cattatttga acaggcaagg cctatattag aacaaattca agacaatggt tttgaagcat
     1321 attatgtagg tggctctgta agagattatg tcatgggaag aaatattcat gatatagata
     1381 tcacaacaag tgcaacgccg gatgaaatag aatctatctt tagtcatacg atacctgtag
     1441 gtaaagaaca tggcacgata aatgtagttt ttaatgatga aaattatgaa gtgacaacat
     1501 tccgggctga agaagattat gtcgatcacc gtagaccaag tggtgttaca tttgttcgtg
     1561 atttatacga agatttgcaa cgacgagatt tcacgatgaa tgcgatagca atggatacag
     1621 catacaaatt gtatgattat tttgatggtc aacaagatat taataatcga ataataagaa
     1681 ctgtaggtat agctgaggaa cgattccaag aagatgcttt acgtatgatt cgatgtttaa
     1741 ggttccagtc acaattatca tttgatattg caacggaaac attcgaagcg atgcgtatac
     1801 aaatggcaga tattaaattt ttatcaattg agcgtatagt gattgaacta actaaattaa
     1861 tgcgaggtat taatgttgaa aagagtttta atcatttaaa atcgctgaaa gcatttaatt
     1921 atatgccgta tttcgaacat cttgatatga atcaaattaa tgtaactgaa gcaattgatt
     1981 tagaattgtt gattgctata gtatcagtta aatttgatat taattactca ttgaagcctt
     2041 taaagctaag taaccgacaa gtaaaagata tcaatcaata tattcaaatt atgaatgcat
     2101 taccaagtat tattacaaaa gaacaattaa aaatgtttgt ttatgattat gatacgcatc
     2161 tcattaaaaa tgtaatggtt gcagcagacg tgataaaggc aaatgatatt caaggacatg
     2221 aaccattaat cgttaacctt caaacgattg atgaaacatt acatcgttta cctatgcata
     2281 atagaaaaga catgatggtt aatggcggtg tacttatggc acatttaaat gccaaaagtg
     2341 gtccgtggtt aaaagatgtg ctaagacaaa ttgagatagc gattgtaaca ggtaaagtaa
     2401 gcaacgaaga aactgaaatt ttgaaatggg tggataatca tgtcaaaata tagtcaagat
     2461 gtacttcaat tactctataa aaataaaccg aattatatat ctggacaaag cattgcggaa
     2521 tcacttaata tatcgcgcac tgcagtaaaa aaagtgattg accaattaaa gttagaggga
     2581 tgtaaaatag attcagtaaa tcataaaggg catttattac aacagctccc agatatttgg
     2641 tatcaaggta taatagacca atatacaaaa agttctgctt tgtttgattt tagtgaagta
     2701 tacgattcaa tagattctac acaacttgct gcgaaaaagt cacttgttgg aaatcaatct
     2761 tcatttttta tcttgagtga tgaacaaacg aaaggtcgtg ggcgatttaa tagacattgg
     2821 agttcttcaa aagggcaagg actttggatg tctgtcgtgt taagacctaa cgttgcattc
     2881 tcaatgatat ctaaatttaa tttatttatt gcattaggga taagagatgc gattcaacat
     2941 tttagtcaag atgaagtcaa agtgaaatgg ccgaatgata tatatattga taatggtaaa
     3001 gtgtgtggtt tcttaactga aatggttgct aataatgatg gtatagaagc aataatatgt
     3061 ggtataggta ttaatttgac gcaacaacta gaaaactttg atgaaagtat tagacataga
     3121 gcaacaagtg tacaattaca tgataaaaat aaattagata gatatcaatt tttagagaga
     3181 ttacttcaag aaattgaaaa aagatataat caatttttaa cgttaccttt ttctgaaatt
     3241 cgtgaagaat atattgcagc ttctaatatt tggaatagaa cgttgctatt tacagaaaat
     3301 gataaacagt ttaaaggaca agcaattgat ttagattacg atggctatct aattgttaga
     3361 gatgaagcgg gtgaatcaca ccgtttaatt agtgcagata tagattttta acactaaagc
     3421 aaggagagat agctatgggt atggcaacct atgccgttgt ggatttggaa acaacaggca
     3481 accaattaga ttttgacgat atcattcaaa ttggtataac gtttgtgcgc aataaccaaa
     3541 ttattgatac atatcattct atgattagaa caaatttaga gattccgccg ttcattcaag
     3601 cgttaacatc cattgaagaa aatatgctac aacaagcgcc atattttaat caagttgcac
     3661 aagaaatcta cgataagata aaagattgca tttttgtagc tcataatgta gattttgatt
     3721 tgaattttat taaaaaggca tttaaagatt gtaatattca atatcgtccc aaaaaagtta
     3781 tagatacact agaaatcttt aaaatagctt ttcctacaga taaaagctat caattaagtg
     3841 aattagcaga ggcacatggt attaccttag caaatgccca tcgagctgac gaagatgccg
     3901 ctactactgc taaattgatg atattagctt ttgaaaaatt tgaaaagtta ccgttagata
     3961 ctttgaagca attatattat ttaagtaaac agttgaaata cgatttgtat gatattttct
     4021 ttgagatggt taggcaatat gatgcaaagc cattggataa atcgtacgaa aagttcgaac
     4081 agattatcta tcgtaagcaa gttgatttta aaaagccgac aacaaattat aatggcagct
     4141 tgaagtcttt atatagcaaa gctgtagatc aacttggctt aacatatcga ccgcaacaat
     4201 tatatttagc ggaaactata ttagatcagc tcatgcatag tgaaaaagca atgattgaag
     4261 catcactagg cagtggtaaa tcattagcat atttacttgc ggccttgatg tataatattg
     4321 aaactgggaa gcatgtcatg atttctacca atactaaatt actgcaaagt caattgttag
     4381 aaaaagatat tccagcaatg aatgaagcgt tgaattttaa aattaatgca ctattgatta
     4441 aaagtaaaag tgattacatt tcattggggc ttattagtca aattttaaaa gatgacacaa
     4501 gtaattacga agtgaatatc ttgaaaatgc aattgctgat ttggattaca gagacgccgt
     4561 ccggagatat acaggaatta aatttaaaag gtggtcaaaa gatgtatttt gaccagaaaa
     4621 ttgaaacata tgtaccggca cgtcacgatg ttcattatta taattttatt aagcgaaatg
     4681 cacagaacat tcaaattggt attacaaatc atgcacattt aattcattct gatgttgaaa
     4741 attcaatata tcaattgttt gatgattgta tagtggatga agcacatcgc ttaccagact
     4801 atgccttaaa tcaagtgaca aatgagttga gttatgctga tattaaatat cagcttggtt
     4861 tgattggtaa aaacgaaaat gaaaaattgt taaaggcaat tgatcaactg gaaaagcagc
     4921 gaattttgga aaaattagat attgcaccaa ttgatatttt tggcttgaag gctagtatga
     4981 atgagattca tgagttaaat gagcaattgt tctcaacgat atttacgatt atcaatgatt
     5041 ctgatgttta tgatgatgat attcatcgct tccataatgt atttactttt gaaacaaaag
     5101 acattttgaa ggatttacat gcgattatcg ataagttaaa taaaacgcta gaaatattca
     5161 atggcattag tcataaaaca gttaaatcat tacggaaaca gttgttgtat ttaaaagata
     5221 aatttaaaaa tatcgaacaa agtttaaaag ctggacatac tagtttcatt tcgattaaaa
     5281 atttatctca aaaatcaaca atacgtttat atgtgaaaga ttacgctgta aaagatgtat
     5341 taacgaaaca agtattggaa aaatttaaat cactcatttt tatatctggt actttaaaat
     5401 ttaatcattc gtttgaagct tttaaacagt tattcaacaa agatgttcac tttaatacat
     5461 ttgaagttaa cacgtcgtta caaagtgcaa aaaatacaag tgtatttata ccgagtgatg
     5521 tagcatctta tcaatataaa aatatagatg agtatgtagc atcgattgta agctatatta
     5581 ttgaatatac gactataacg tcatcaaaat gtttagtctt atttacgagt tataaaatga
     5641 tgcatatggt acaagatatg ctaaatgaat tgccagaatt tgaagattat gttgtattaa
     5701 cacaacagca gaaccaaaat tataaaatag tacaacagtt taataatttt gataaggcca
     5761 tactgcttgg tacgtcaaca ttttttgaag gttttgattt tcaagcaaat ggtattaaat
     5821 gtgtgatgat agcgaagtta ccgtttatga acaagcataa tgcaaaatat tggcttatgg
     5881 attctgaatt cacttcaact ttcaaggaat atgtattacc tgatgcagtt acacgtttta
     5941 gacaaggact tggaagatta attagaagtg aaaatgatcg cggcataata gtttcatttg
     6001 atgatcgact tataaacagt aattataaaa acttttttga acaaacactt gaaaattatc
     6061 gtcagaaaaa aggcgacatt caacagtttg gaaaattatt aagacaaatt caaaaaaaga
     6121 aaaagtgaaa tacaaagtaa aattcttgtg acatgacatg tgctatgttc tgttacaaga
     6181 atttttgttt ggtcgtcaaa atttgagttt tataattaaa cttgaatgtt agcacaaaaa
     6241 acaaccttca tactaataat attgaggctt tattatatat tttgtctgaa actgatgatt
     6301 gtattgtctt aatataaatt aatgttcaaa tctgtgctta aatcattacc atttacacgt
     6361 tatcttttag tctagttcta gaacttgaaa gcggattttg ttaaaatgaa tgagtattgt
     6421 ataacgaaaa aataaatagg agaatggtta tgaaaacaac gattaaacaa gcgaaagatc
     6481 atttaaacca agacgttaca attggtgctt ggctaacaaa taaacgttca agtggtaaaa
     6541 tcgccttttt acaattacgt gatggaacag gctttatgca aggcgtagta gttaaatcag
     6601 aagttgatga agaggtattc aaacttgcga aagaaattac tcaagaatca tctctatacg
     6661 ttacaggcac aattacagaa gataatcgtt ctgacttagg atacgaaatg caagtgaaat
     6721 caattgaagt tatttcagaa gcgcatgact atccgattac acctaaaaat catggtacag
     6781 aattcttaat ggatcaccgt catttatggt tacgttctaa aaaacaacat gctgtaatga
     6841 aaattagaaa tgaagttatt cgtgcaacgt atgaattttt caacaaagat ggatttacaa
     6901 aggttgatcc accaattttg acagcaagtg cgccagaagg tacaagtgaa ttattccata
     6961 ctaaatactt tgatcaagat gcgtttttat ctcaaagtgg tcagttatac ttagaagctg
     7021 cagcaatggc acacggaaaa gtattttcat ttggtccaac tttcagagct gaaaaatcaa
     7081 aaacacgtag acacttgatc gagttctgga tgattgaagg ggaaatggct ttcataaatc
     7141 atgctgaaag tttagaaatt caagaacaat atgtaacaca tgttgtaaaa tcagttttag
     7201 aaaattgtaa actagagttg aaaattttag agcgtgatac atcaaaactt gaaaaagttg
     7261 cgacaccatt ccctagaatt tcatatgatg atgcaattga attcttaaaa tcagaaggct
     7321 ttgatgatat tgaatggggc gaagattttg gtgcgccaca tgaaacagcc attgctaatc
     7381 attatgattt accggtgttt attactaatt atccaactaa aattaagcct ttctatatgc
     7441 aaccaaatcc tgaaaatgaa gaaactgtat tatgtgcaga cttaattgca cctgaaggat
     7501 acggtgaaat tattggtgga tctgaacgtg tggatgactt agaattgtta gaacaacgcg
     7561 ttaaagaaca tggattagac gaagaagcat atagttacta cttagactta cgtcgttatg
     7621 gtagtgtgcc acactgtgga tttggtttag gtttagaacg tacagtagca tggatttctg
     7681 gtgttgaaca cgttcgtgaa acagcgccat tcccaagatt attaaaccgt ttatatccat
     7741 aagttttagg gtcgttactt aaaatatata ttatgataat aaaaagaatc gtccagccta
     7801 tattaatagc tgggcgattt atgcttggaa aatgaagatt gataacggct tatattaatc
     7861 atgaaaaaac ttttgtttag taactatatt tggaaaataa atagttcatc t
//
LOCUS       DAMZLE010000104         7859 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMZLE010000104 DAMZLE010000000
VERSION     DAMZLE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.105"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(142..978)
                     /locus_tag="POB92_002045"
     CDS             complement(142..978)
                     /locus_tag="POB92_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDI6579517.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGIIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(978..1592)
                     /gene="ureG"
                     /locus_tag="POB92_002046"
     CDS             complement(978..1592)
                     /gene="ureG"
                     /locus_tag="POB92_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDI6579518.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFIINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(1605..2294)
                     /locus_tag="POB92_002047"
     CDS             complement(1605..2294)
                     /locus_tag="POB92_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDI6579519.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2287..2739)
                     /gene="ureE"
                     /locus_tag="POB92_002048"
     CDS             complement(2287..2739)
                     /gene="ureE"
                     /locus_tag="POB92_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDI6579520.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(2752..4467)
                     /gene="ureC"
                     /locus_tag="POB92_002049"
     CDS             complement(2752..4467)
                     /gene="ureC"
                     /locus_tag="POB92_002049"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDI6579521.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4464..4874)
                     /locus_tag="POB92_002050"
     CDS             complement(4464..4874)
                     /locus_tag="POB92_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDI6579522.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(4888..5190)
                     /locus_tag="POB92_002051"
     CDS             complement(4888..5190)
                     /locus_tag="POB92_002051"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDI6579523.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5450..6355
                     /gene="yut"
                     /locus_tag="POB92_002052"
     CDS             5450..6355
                     /gene="yut"
                     /locus_tag="POB92_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDI6579524.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            6572..7726
                     /locus_tag="POB92_002053"
     CDS             6572..7726
                     /locus_tag="POB92_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDI6579525.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELEGVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNSTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
BASE COUNT         2513 a         1319 c         1350 g         2677 t
ORIGIN      
        1 aaacgaagtt atttattcag cattgggaca taaaattaac ttaaaattta aatattgaag
       61 atgctttaat taaagttaaa gactagccat accttatttc agcttattaa gcttgaaaca
      121 aggtacacta gtctttttat tttaatattt tcttagaaaa tcaagtttac gatcataaat
      181 attttctgcg atatagcttt ggatgattcc aagtattttc tctataattt gtgtgcgata
      241 agcaaaaatt ctaactgcaa aaccatgtgt aggcaattga gaaatagcaa cacgacaatc
      301 gaatgtattg ctataagaac taatggtttc ataaactgaa tcgattagtt tttggttaac
      361 atcttcatgt atgaagtaag cagagccgta atgagagtaa tgttccatat aacctatttc
      421 attgatcgat tgtttattag gattcaataa taaattatca tatgtgacta actcatcttc
      481 aatataaatt tcatttatta aatgcatata ttgatacttg aaggcttcgc cagtttttga
      541 ataaccagga gttaaaatat cagtataaaa taatgaacta gaattattga gattgaacgt
      601 attgtgttga taaaatttag cattttcata tgcgatgatt ggatcagcga catattctaa
      661 atatgcgtta tctttcaaat taaaagtttg atactgctca acatgattgg aaggtgtctt
      721 gtatattttt gttgcacctt gagatgtcaa tgtcacttta gcgttatctt cgacattcac
      781 attcatacgg taacgatctc catctaaata accaccacct acatttacta tataaaatgt
      841 aggaatggta gattgattta gataaactgg acgtatcact ttaagtgctt tttcaaagaa
      901 aatatctctt gatactgatc ggttgccatc aaaaaacact gttaaatcaa gttgcccagt
      961 ccattgttgt tcatccatta tgataatcct ttgagtaaag tgtcgcgttc aatccaatcg
     1021 ataacttcat ccaaaccttc gtcagttttt aagttagtaa aagtaaatgg acgtttacca
     1081 cgaaatactt tagtatcttc agccatttgt tctaatgatg cacctacata gggagctaaa
     1141 tcagttttgt taattataaa gaaatctgac ttaatcatac cttgaccacc tttacgtgga
     1201 atcttttcac cttgagcaac atcgataata tatattgaaa agtcaacaag ttctgggcta
     1261 aatgttgctg ctaagttatc gccaccagat tctatgaaaa ttagttctat atcgtcatga
     1321 cgttctaata attcgtctat tgctgcaaag ttcatagatg catcttcacg aatcgcagta
     1381 tgaggacatc caccagtttc aacaccaatg atacgacttt caggtagaac tcctgaattt
     1441 actaatatct tttcgtcttc ttttgtatat atatcatttg taataacgcc gatactcatt
     1501 tcttttgaaa gacgttttac aactttttca attaattgtg ttttacctgc acctacagga
     1561 ccaccaatac caattttaat cggatttgcc acaattataa cctcctatga tatgaaaatt
     1621 ctaacattga cgttctcatg cgccatttga tttagttcta aaccaggcgc tgtcatgcca
     1681 aaatctgctt cttttaattc gaaaatctgc tttcttgttc cttctatata aggaatcatg
     1741 tgagtaacta tcttttgacc agcagtttgt ccaagtggaa tagcacgaac agcattttga
     1801 gttaaacttg aaacattttg atataaatag taatcaataa tcgtttcaat atctacacct
     1861 aaatgatggc ctagcatagt aaaacaaata gctggattta actttgcttt cttatcttgc
     1921 atttgttgat gataccaagc aatccatggg ctattataaa gttctaaagc caatttaacc
     1981 atgcgagtcc ccatttgttt tgcaccaaca cgtgtttctt taggtaagtt ttgaacaaac
     2041 atcagtttat ctatgtgtaa tactttttgt gtatcatcat tttccaatgc atcataaact
     2101 aaacgcatgg ctaaaccatc agaataggta agttgctctt gtaaaaacat ttttaaccaa
     2161 gcaataaaag tatgatcgtc atgaattata tttcgttgaa tatatgtttc aagaccaaat
     2221 gaatgactga aagcacctgt tggaaactgt gaatcacaga actgaaataa tcttaagtgt
     2281 gtatgatcaa tcatgagaat gccctatatg tctgaaagcc ttattaactt tacggtcttc
     2341 tcgaacatat gggatgccta aactttttaa taaatcttca actaaataat catattgtac
     2401 tagcatttca gtctctgtaa attgtgctgg caaatgacga tttcctaatt gatgggctat
     2461 atctcccatt tcttgcaatg ttcttggttg aatcactaaa agatcttctg aattaacatc
     2521 cacaataatc atattatggt catctgcgta taaaatatct ccatattgta agtcaatagg
     2581 ttgttttaaa cgaatgccta tttcagtgcc atggtctgta acgactcttt gaatacgttt
     2641 aacaagatct gaattttcaa ggtatacttt ttcgacgtgc ttttgttttt ctgaatttga
     2701 caaattggca atattgcctt ggatttcttc aacaatcatt ctatgttcct cctagaataa
     2761 gaagtatctt tgagttaatg gtaactcagt tgctgcatta cttgtaattt tttctccatc
     2821 tacatatact tcatatgttt gtggatcaac gtctaattta ggtgttgcgc tgttattttt
     2881 catatctgct ttagataatt gtctaatatt tttaactgga cgcaccatgc gttttaaatt
     2941 taatgcacga ttgataccat tttcataagc agttttagac acgaatgtca ttgacgtact
     3001 tgtaaggttt ccgccgtatt gaccatacat tttacggtac ttcatcggtt cagatgtagg
     3061 tatagaacca tttgcatcgc catttacggc agagttaatt aatccgccct ttacaactaa
     3121 ttcaggttta accccaaaga aaattgggtc ccataagaca atgtcagcta gtttgcccgg
     3181 ctcgatagat cctacatatt cagaaatacc atgtgtaatt gctgggttaa ttgtatattt
     3241 agcgatataa cgtttgatgc gattattatc attatgttca aaatcaccat ctaaaggacc
     3301 acgttgttct ttcatgcgat gtgctacttg ccatgttcgt gtaattactt cacctacacg
     3361 gcccattgct tgtgaatcgg aactaatcat actgaataca cccatatctt gcagaacatc
     3421 ttctgctgca atcgtttctt tacgaatacg tgaatctgcg aatgcgatat cttcaggaat
     3481 agccgcattt aaatggtgag taatcattac catatctaaa tgttcatcta cagtattatg
     3541 tgtataaggc aaagttggat ttgtagatga aggtaaaata tttgaaaatg cagctgattt
     3601 aattaaatca ggcgcatgac cgccaccagc accttcagta tggtacatat gaagtacacg
     3661 gtctttaaca gcagccattg tgtcttccat aaatcctgct tcatttaaag tatctgcatg
     3721 taatgcaatt tgaacatcaa attcatcagc aacatctaat gcatgactca aagcagatgg
     3781 tgttgcaccc cagtcttcat gtacttttaa tccaattgct ccggcattga tttgttcaat
     3841 gagtgcagtt ggatttgttg cttgtccttt acctgtaaaa ccgacattaa tcggtaaacc
     3901 ttcggcagct tctaacattc tatgaatatg ccatggacct ggagttacag ttgttgcttt
     3961 agaaccttct gaagcaccag taccaccacc aatatgagtc gtaatgccac tttctaatgc
     4021 gacctctgct tgttcaggat taataaaatg aacatgagta tcaataccac cagcagtgac
     4081 gattttacct tcagcggcaa tgatatctgt tgttgaacct ataataatgt cgacattatc
     4141 cattatatct gggttgccgg cattacctat ggcgaaaata taaccatttt taatgcctat
     4201 atcagcttta accactttat cgtaatcgat aataacggca ttagaaatga caaggtctgc
     4261 aacgttcacg tcatcacgtg ttacacgagg attttgcgcc ataccgtctc taatagattt
     4321 accaccacca aaagtagctt cttcaccata aaccgcatag tctttttcta tttgagcaaa
     4381 tagattcgta tcacctaaac gaatggaatc tccaacagtt ggaccgtata agctcgtata
     4441 ttgattttgc gtcattttaa agctcatgat ctttttcctc cttttttatt cacgttttca
     4501 gcaccgttat ctccgaatac acctgcatat tcatcatttt catcagttgg gcgatagaca
     4561 cgtgactcat cgataggacc attgaccata ccacgaaaac caaaaatttt acgtttgcca
     4621 gcatattcaa ctaattgaac ttctttttta tccccaggtt caaatcggac agctgctcca
     4681 gctgggatat ctaaatgttt tccatatgcc atttcacgtt cgaaatctaa tgctgcattt
     4741 gcttcataaa aatgaaaatg tgagcccact tgaattggtc gatctcctgt attttcaact
     4801 tcgataactg tttcaggatg atggttatta atttcaacct ctgtactttt tgtaataatt
     4861 tctccaggta tcatttgact gcctccttta aacaataggg tgatgtactg tgattaactt
     4921 agtaccatcg gggaacgtag cctcgatttc gatatctgta atcatgtgtt cgacaccatc
     4981 catgacatct tctttgttta gaatttgtct accataactc attaactctg caacggtctt
     5041 accatcgcgt gcaccttcta ataattcatc gctgattaaa gctaatgcct caggatgatt
     5101 tagtttcaaa ccacgtgctt tacgacgacg tgcaacttcc gccgccacta caatcattaa
     5161 tttgtcttgc tctcgttgtg taaaatgcaa attaaaaccc ccaatttcat attagataca
     5221 atttacaaaa tttatattaa tcctaattgt tgtgataaac aagtaatata caaagttcaa
     5281 tgtgtaatta gaaaattata tttttagcat atccgaaatt gaagcaaaca atctaatcga
     5341 aaacaaatag tggaatatat ttatgtaaaa accaaaatag tttttaatat aacttttcat
     5401 agaatagtag tatattaatg agtaatgatt caaaggaaag gtgaaagatt tgaagataat
     5461 agatgtactt ttgaaaaata tttctcaggt tgtgttaatt agtaataaat ggacaggatt
     5521 atttatctta ataggattat ttgtagccga ttggacaatt ggattagcgg ctattgtagg
     5581 tagtatcata gcttatgcat ttgcacgtta cattaattat agtgaagcag agattaatga
     5641 tgggttagcc ggatttaatc cagtgttaac tgccattgcg ttaacaatct ttttagataa
     5701 gtcaggatta gatattgtta taacaatgat agcaacttta ttaacgttac cagttgctgc
     5761 tgcagtgaga gaagttttaa gaccatataa agttccgatg ctgacgatgc cttttgtcat
     5821 tgtgacttgg tttacaattt tactttcagg acaggttaaa tttgtagata catcgttaaa
     5881 gttaatgcct caaaacattg aaacggttaa ttttagcaac aatgatagaa tacatttcat
     5941 tcagtcatta tttgaaggat tcagtcaagt atttatcgaa gcgagtgtaa ttggtggcgt
     6001 atgtatttta atcggcatat tgatagcatc aagaaaagca gcactcttag ctgttatagc
     6061 tagtttgtta agctttatca ttgtagctct attaggtggt aattatgatg atattaatca
     6121 gggattattc ggttataact ttgtattaat ggcaatcgca ctaggatata catttaaaac
     6181 agcgattaac ccttatattt cgactttttt aggtgtgtta ttaacagtag tggtgcaact
     6241 aggtacaaca acattgcttg aaccgtttgg cttacctgca ttaacattgc catttattat
     6301 tgtgacatgg attttattat ttgctggtat taaacatgac aaagtagatg cttgatagtt
     6361 aaatcaaacc taatattgtt tgaatatcac cttaaactat acagcgaatt gtatagttta
     6421 aggtgtattt ttatggataa aattaagtgc atacttaatc gaattatcag ttttctttta
     6481 attgttcaca gcacgttcct ttttattttt aaagtcgagt gttaaactag gaataaataa
     6541 tttatattgt gtgttgtgtg gggtgactaa tatgaatgat atggataatt cctttttaat
     6601 aacaacggaa attcaaagaa aatggattga aaaattcaaa gtaattagag atacatttaa
     6661 ggctaaagct gaatataatg atcaacatag ccaatttcca tataaaaata ttgaatggtt
     6721 aattaaagaa ggttatggaa aattaacgtt accaaaagca tatggtggtg aaggtgcgac
     6781 catagaagac atggttattt tgcaatcatt tttaggcgaa cttgatggtg ccacagcatt
     6841 atctattggt tggcatgtga gtgtcgtagg acaaatttat gaacagaaat tatggtctca
     6901 agatatgttg gagcaatttg ctgttgaaat taataatggt gcattagtta atagagcagt
     6961 tagtgaagct gaaatgggta gtccaacaag agggggaaga ccaagtacac atgctgttaa
     7021 agctgatgat gggtatattt taaatggtgt gaagacatat acatcaatga gtaaagcact
     7081 aacacatatt attgttgctg cttatataga agaattagaa ggtgttggtt ttttcttagt
     7141 ggacaggaat ttgcctggtg ttgaaattgc cgataattgg gacgtattag gtatgcgagc
     7201 gacagaaagt catgatttaa tattaaatga tgtaaaagta ccattaaaac atttagtgga
     7261 aacagagaaa agtaaagcgc caaatggttg gatattgcat ataccaagct gttatttagg
     7321 tatagcacaa gctgctagaa attatgctgt agactttgca atccaacata gccctaatag
     7381 tattgaagga acgattgcaa cattgccaac tgtgcaacaa aatttaggga aaatggaaac
     7441 gctattgtta tgtgcgagac aatttttatg gagtacagca aaaggctatc aacaatataa
     7501 agatgacagt cagataagaa attcaacaag tgcgagtaaa gtgatggtaa tgaatcaagg
     7561 gcttgaagtg attgatttag cgatgagaat agttggtgct aaaagcttag aaatgaatcg
     7621 accattacaa cgttactatc gtgacatgcg tgcaggatta cacaatccac ctatggaaga
     7681 tgcagcatat acaaatattg ctaagagtat tacagataca ttttaaattt aaaaaggtaa
     7741 atcgatcgtt gattggcata aagagatgaa atttgacgtt cggatttacc cttttttgtg
     7801 gggatttata gggatgttaa gatattttgt taaatttttt gcgaaaataa aaaaataaa
//
LOCUS       DAMZLE010000105         7848 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMZLE010000105 DAMZLE010000000
VERSION     DAMZLE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.106"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(260..1087)
                     /locus_tag="POB92_002054"
     CDS             complement(260..1087)
                     /locus_tag="POB92_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6579526.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(1090..1209)
                     /locus_tag="POB92_002055"
     CDS             complement(1090..1209)
                     /locus_tag="POB92_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579527.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1321..2322
                     /locus_tag="POB92_002056"
     CDS             1321..2322
                     /locus_tag="POB92_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDI6579528.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2444..2908)
                     /locus_tag="POB92_002057"
     CDS             complement(2444..2908)
                     /locus_tag="POB92_002057"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDI6579529.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAIITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2921..4102)
                     /gene="ribB"
                     /locus_tag="POB92_002058"
     CDS             complement(2921..4102)
                     /gene="ribB"
                     /locus_tag="POB92_002058"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDI6579530.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(4113..4745)
                     /locus_tag="POB92_002059"
     CDS             complement(4113..4745)
                     /locus_tag="POB92_002059"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDI6579531.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4752..5795)
                     /gene="ribD"
                     /locus_tag="POB92_002060"
     CDS             complement(4752..5795)
                     /gene="ribD"
                     /locus_tag="POB92_002060"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDI6579532.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKITKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5922..6057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6276..7778)
                     /locus_tag="POB92_002061"
     CDS             complement(6276..7778)
                     /locus_tag="POB92_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6579533.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVYRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHKVSQLYDALESNEQQQRSDNQ"
BASE COUNT         2502 a         1439 c         1093 g         2814 t
ORIGIN      
        1 cttcaaaaaa gatacgcatt gaatttttat tacatataac tctcatgaaa attgattaaa
       61 ctaaattaat tagttagcct taaatttaac aaattaaatc aaaccgaatg aggctaaatt
      121 ggttattttt caagatatga tttgtataaa tcaaaacatt tcttacaaat caaatgtgta
      181 taaaatgtga tatacattct caaccttaaa aaagttctaa aaaaaagatg agcatctact
      241 gtctcatctt tttagaatat taaatttcta caatttcgtc atcttcaaca ataaagccca
      301 ttgtattgac gctgttattt aagaaagtca gaatataacg cattacttca tcgcgttctg
      361 gctcattgtg aacctcgtgg taaaaacctt gccaagcttt aaaatataat tcaggtgttt
      421 gatatttttc tttaaactca tcaattgccc tagtatcaac aattaaatcc ttcgttccat
      481 acattaatag cgttggcatt ggttgaatgt catgaatatg agccatcgta tctttcatcg
      541 tctcattaat tgtattatac caatgatacg ttgctttttt taacattaaa ccatcgttaa
      601 ctgtttcttc aacaatttct aaattacgtg ttaaatcttt tggttctaca ccaacattaa
      661 aacgtgtgtc ttttgaaatt ttacctatat ttgaaacaag tttatcttta cgattttttc
      721 cattcttttg aagttctagc ataggagaaa ttaacatcat cccctcgatt ggcaattcta
      781 ctttttcaag taaatttaat aaaatcaaac cgccaagtcc tacccctaat acataagtag
      841 gaattttata ttcattagct atctttaacc agtctagcaa actttcgtga tacgtttgaa
      901 agttttcaat ttgtccttta ttagctcttg aagtttgacc ttgaccaggc aaatctccca
      961 taatcacatg atagccattt cttcttaaca tcgtaataac atatgcatat cttcccgtat
     1021 gttctaatat attatgagca ataacaacga cgcctttcgc atcattttca gcttcccact
     1081 tccacattat tatactgccc ctttttcatt aatcttcaat aacataatta tagcaaattc
     1141 actatgtaga tttctattta tagtattatt gttgtccata ttattatata taaatgaaat
     1201 caacatcaat gatagtgtaa ttatacataa ttatttttga ttgtttttga tgaaaacgct
     1261 ttctcgaata tttttttcat gctaaactta ttgtaaacac aagggtttgg aggagtagca
     1321 atggcactat taaagaattt ttttatcgga ttatctaata atagtttttt aaacaacgca
     1381 gcaaaaaaag tgggcccacg tttgggcgcc aataaagtcg ttgccggaaa tacaattcca
     1441 gagttaatta atacaatcga atacttaaat gacaagaata tcgctgttac agtagacaat
     1501 ttaggggaat ttgtcggtac agttgaagaa agtaatcatg ctaaagaaca aattttaaca
     1561 attatggacg cgcttcatca acatggcgta aaggcacata tgtctgttaa attgagtcag
     1621 ttaggtgcag aattcgactt agaattagct taccaaaatt taagagagat tttacttaaa
     1681 gcaaatactt acaacaatat gcatataaat attgatactg aaaaatatgc tagcctgcaa
     1741 caaattgttc aagttttaga tcgattaaaa ggcgaattta gaaatgttgg tactgtaatt
     1801 caagcatatt tatacgatag ccatgaatta gttgataagt accaagattt acgattacgt
     1861 ttggttaaag gtgcatataa agaaaacgaa tcaattgcat ttcaatctaa ggaagacgta
     1921 gatgcaaatt acatcaaaat aattgaacaa cgtttgttaa acgcacgcaa tttcacttca
     1981 attgcaacac atgaccatcg catcattaat catgtaaaac aatttatgaa agaaaatcac
     2041 attgaaaaag atcgtatgga attccaaatg ctctatggtt ttagatcaga gttagcagaa
     2101 gaaatcgcaa atgaaggcta taatttcact atttatgtac cttatggcga tgattggttt
     2161 gcgtatttta tgagaagatt agcagaacgc ccacaaaacc tatctcttgc tgtaaaagaa
     2221 tttgtgaaac ctgctggctt aaaacgtgtt ggcataattg cagctttagg agctacagtt
     2281 atgttaggtt taagtacaat taaaaaatta tgccgtaaat agagcaagac ataaacaata
     2341 atttaggagt ctggaacaat aatcaatgtt ctaggctcct aaatgttata ttggcagttc
     2401 tgaaacattt ataaatcttt gattatgaac tcacaacagt gctctatgct tttatagatt
     2461 ttaataaatt agccatttca attgcactta ctgctgcttc ggcaccttta ttaccagctt
     2521 tcgtacctgc tctttccaca gcttgttcaa tactttcagt cgttaaaata ccaaatatga
     2581 ctggtacatt agtttgatca tttactttag aaacaccttt cgcgacttca ttacaaacat
     2641 aatcataatg agacgtagca ccgcgaatta cgcatcctaa tgtaattatt gcatcataat
     2701 ttcctgatga ggctaatttt ttagctacta aaggaatttc aaacgcacct ggcacaaatg
     2761 ctacatcaat attgtcttca ttaacatcat gtcgaatcaa agtatctttt gcaccttcaa
     2821 gtaatcttcc agtgataaaa tcattaaatc gactaactac gattgcaact ttcaaatctt
     2881 ttccaattaa tttaccttca aaattcatgt taaaatcctc ctatattaaa tgacccattt
     2941 ttattttttt cgtttccata taatcatgat tatgtaccgt ttctggtacg ataacttcaa
     3001 ttctttctgc aatatcaatg ccatattgtt ttaatccctc aaatttactt ggattattac
     3061 ttaataaatt gatatgttcg atgttaaaat attttaaaat ctgtgcagca atatgataat
     3121 ctcgcaaatc ttcatcaaaa cctaatgcta aatttgcagt tactgtatca tatccttgct
     3181 caattaattc atatgcgcgt aatttgttta acaatcctat gccacgacct tcttgaggta
     3241 gataaataat catgccacca tgttcattga tatacttcat agacgattca agttgagcac
     3301 cacaatcaca acgttgacta tggaaaatat cgcctgtaag gcacgcagaa tgtaagcgta
     3361 cattttcatg ttgtcgaatt gcaccttttg tcagtacaac tatctcttca tctgtgtaag
     3421 tcgctttaaa accatacata tcaaatgttc cgaaatctgt aggcattttc acttttgcct
     3481 taaattcaat ttctggttct aattttttac gatattcaat taaatcatca atcgtaatca
     3541 tctttaattg atgtttttct ttaaactttt gtaaatcttg tcctttcgcc atcgtgccgt
     3601 catcattcat aatctcacaa atgacaccag cgggcttggc accagtaagt ttagctaaat
     3661 caacagccgc ttctgtgtgt ccatttctag ctaatacgcc tttatcttgt gctactaatg
     3721 gaaataaatg accaggacga ttaaaatctt tagcttcact actaggatca atgagctttt
     3781 tggcagtcaa tgtacgttca taagcactaa ttcctgttgt tgtatctaca tgatcaatac
     3841 ttactgtaaa ttgcgtacca aagatgtcgg agttatcatc aaccatttgt accaaatcca
     3901 aacgttgtgc aatatcttta gacactggtg cgcatattaa tccccttgct tctttcgcca
     3961 taaaattaat ggtattatcg ttcatccatt cagtgaccgc tactaaatca ccttcatttt
     4021 cacgattctc atcatctact acaataattg tttctccatt ttttaaagcc attaaagcac
     4081 tgtcaatatt atcgaattgc atgctacccc tcctaaaaac caaatgctct taatttatct
     4141 acagataatt ggtctttatc tttatttaaa atattttcaa catatttaaa caaaacgtct
     4201 gtttctaaat gtactttatc tcctaatttt ttggatgata aaatcgttga acgcctcgtt
     4261 tctggaataa gatgaatgtc aaaactgtta tcatgcttat caaataccgt tagacttaca
     4321 ccatccacag taatagaccc ttgcttaact aactgattat taatatgttg gctacattga
     4381 atcgtaataa ttttggcatt ggctgtttca tttatttttg aaactgttcc tagttcatct
     4441 acatgaccga ggacaaaatg tccaccaaac ctaccgttac cactcatggc acgctctaaa
     4501 tttacttctg attgtcgctt aacatctgct aaataggttt tattttcagt gcctttaatt
     4561 acttgaacag taaaagatgt ctgatcaaaa tcaatcactg ttaaacatgc accattaaca
     4621 ctgatggaat caccaatatg catatctgcc gtaatcttat gtgcttcgat ttcaatcgtc
     4681 ctgactgatt gacgaatttg aacactttta acgacaccta tttcttcaac gatgccagta
     4741 aacatgcatc atcacttctt tcgtaaagtt aatttaacat tttgatttaa taactcggaa
     4801 tgaacaattt caaattggtt cgcatctggt atctcaatca catcatttgt ttgataaaat
     4861 tgataatttc cagatccgcc aattaatttc ggggcataat agagaataaa ttcatctata
     4921 taattagatt ggagaaattc tgaagtagtg gttggacctg cctcgactag caaagttcca
     4981 actcctcttt tatataaatt gtgaagaatt gttgttaaat cgcaagactt caagtaaata
     5041 atttcaatat gtgtttgatt ggttgttaaa tttggatttt cagtatatat ccaaattggt
     5101 gttgattcat cttgataaat ttgctgatta aaatgaatat tcccagactt agacaatatt
     5161 acttttatag ggttttttcc atcttgaata cgtgtagtat attgtggatc atctaattca
     5221 actgtacgtc ttccagttaa cactgcgtcg tgtcgatgtc ttaacttata gacatcttgt
     5281 ttaacctctt tgttagtaat ccattgactt tgtccattat cattcgcttg tttaccatct
     5341 aaacttgcag atactttcac tgtaatttgt ggcagttgct ttgcttttgc tttaaaaaag
     5401 tcttggtata attgtgatgc ccgttcatca tcaacgcatt caacctcaat accgtgagcc
     5461 cgtaacgtct catcaccatg tgtgtctaac gaattgtctt ttgttgcgta aactacttta
     5521 gttatcttac aatcaataat tttgttaaca cagggtggtg ttgaaccaaa atgactacat
     5581 ggctctaacg taatataaat cgtcgcacct tcagcatttt gttgtgccat atcaagtgct
     5641 tgaacctccg catgcttgtc accttttctc aagtgtgcac caataccaac aatcctacct
     5701 tctttaacta caacagcgcc aacgggtgga ttaacacctg tttgaccttg taccatattt
     5761 gcaagttgaa tcgcataatc cataaattga ctcaaatgat cacctctata aacaaaaatc
     5821 ctcacatcat gaattaagat gcaaggagaa aaatttatcg ttaaataagc ctatttgtac
     5881 acatttttac aaatacgcta cattatcttt gtcgataatt aacattcttt ctcccatcca
     5941 gactttaact gtcggctcta gaatctcact agatcagcca ctaatatgaa acatattagc
     6001 aggtcgcagg ctttatttac tgccggttgg gaatttcacc ctgccccgaa agaattatat
     6061 atgaaattgt tatagattat ttgagtacgt agtatgtcaa ctacatttaa aatgatacta
     6121 tatgttttct gaaaaaacaa ttaatgacgg ttttaattta atataatctg agtactatag
     6181 gcatctcatt gatatgattc ttactaacag acattaaaat caaaccttca attcgtctct
     6241 atagagcgtt ctctttatta tcttctagtt acaaattatt gattgtcact gcgctgttgt
     6301 tgctcattcg attctaaagc atcatataat tgagatactt tatgcgcaac ttgttctaca
     6361 atcattttca caccatttcg tagtttatta acaccgtttg tcatttgacc tatcgcaatc
     6421 atatttgtta atgttccaaa ccttggacta ataacttgat tggtttccgg aatgatttgt
     6481 atgcctccca ttgggtgtgc ttgtacaatt tgtctatttt caagattttt aattaattga
     6541 tcatcttgat ccaattcatt taaatgactt tttgcacctg tcgcgttaat gacaacatta
     6601 tatatgtcta ctgattcttg gtttttgtat gaaaaataat acaacttgcc atcatgtttc
     6661 acatcttcta aatctttttt caaaattaaa gacttatttt ctattaattc aataattagt
     6721 tcagcagttc ttggaggcat tggatttgaa tttaattgaa tcatctttga gtatttttga
     6781 ttaaattgat gttggtcttc aatacttaag ctattccata tccaatttaa attctctttc
     6841 aaatgttcaa tcatactttg gaaaatgccc atttctgttg gacgcgctaa atcatacttc
     6901 aaatctgcaa tatgatttcc tgtacgtcta tatactaatt ttttaaaatc aatgtcatat
     6961 tcagcacatt cttttaaaaa taaagaaact aaagtatcaa gcggtgcatt gccgaaatga
     7021 tgttttttaa tgtcatttaa tttgtcttta gttaagtact tgaatgtcac gtctatcatt
     7081 gtacctctta cacttggtaa atgagcagaa cgactcgtca tagtaattgg taatttcgga
     7141 tgatgagcag caacataacg gacaacatct aaactggcaa ggcctgtacc aataatcgca
     7201 atatcgtcca gttcatttac ttcgtctaac gtattatatg ttggataagg cgtagcgata
     7261 tatccttttt taccctttaa gttatatgga tcatggtagg caaatgtacc acatgttaaa
     7321 aatacataat cgtacgcttg ccatgattgt tctgaatttg tagtacatat gtaataagtt
     7381 aaattcgttt catcgatatt agaatttgta taaatctctt gaactttatt ataattagtt
     7441 gatatatttg gatatttttt cgtgaacata gataaataag atttcatata atgtccgaat
     7501 acaaatctcg gtaaatatgc aggttcatca aaattaaaat cagtttgttg tttataccac
     7561 ttccaaaatt cagtctcatc atctaaattt aaactcatct ttttcgaagg catattaatt
     7621 agcagctcag aactatcatt ttgaaatggt acgccctgtc ccatatttac tttatcatcg
     7681 tataaatcta tatctaattg attaaacttc gggtgcttaa ctaactctct caatacactt
     7741 acaccagcag ttcccatgcc tattattgct acacgcatat atcatccatc cttttgtaat
     7801 tcaaaatatt atatttaaac tattacctat aattatagca atttggta
//
LOCUS       DAMZLE010000106         7800 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMZLE010000106 DAMZLE010000000
VERSION     DAMZLE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.107"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(91..408)
                     /locus_tag="POB92_002062"
     CDS             complement(91..408)
                     /locus_tag="POB92_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI6579534.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(744..1424)
                     /locus_tag="POB92_002063"
     CDS             complement(744..1424)
                     /locus_tag="POB92_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDI6579535.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILLARSSD"
     gene            complement(1496..2083)
                     /locus_tag="POB92_002064"
     CDS             complement(1496..2083)
                     /locus_tag="POB92_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDI6579536.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILFLVISISMILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLILMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(2073..2648)
                     /locus_tag="POB92_002065"
     CDS             complement(2073..2648)
                     /locus_tag="POB92_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDI6579537.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(2662..3906)
                     /locus_tag="POB92_002066"
     CDS             complement(2662..3906)
                     /locus_tag="POB92_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579538.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIAKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLRQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLTEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(3913..5211)
                     /gene="aroA"
                     /locus_tag="POB92_002067"
     CDS             complement(3913..5211)
                     /gene="aroA"
                     /locus_tag="POB92_002067"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI6579539.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(5221..6285)
                     /locus_tag="POB92_002068"
     CDS             complement(5221..6285)
                     /locus_tag="POB92_002068"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI6579540.1"
                     /translation="MKLQTTYPSNNYPIYVERGAIDHISTYIDQFDQSFILIDEYVNQ
                     YFANKFDDILSYENVHKVIIPAGEKTKTFHQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQEIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHEINHYIQYLIQLGYPLDMITDLDFETLYEYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(6311..7477)
                     /gene="aroC"
                     /locus_tag="POB92_002069"
     CDS             complement(6311..7477)
                     /gene="aroC"
                     /locus_tag="POB92_002069"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI6579541.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSKTFKANLDRNDVRVIDDSIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         2517 a         1455 c         1066 g         2762 t
ORIGIN      
        1 ctttaaaaat aatatgattt catgcgatgc aattcattga tggatgtttt taatcttaat
       61 caaatccaaa taaagcatat atttttatat tcactttctt tcgaatcgat ttttatctct
      121 tgtattaaac ttttccatag tttcattaaa gctctctgtc atatctattc ccattgaatt
      181 cgctaaacat aacaacacaa ataaattatc acctaattct gctttaatcg tatttgcttc
      241 ctctgaatct ttcttctttt tttcaccata ggtatgattt atttcacgtg caagttcgcc
      301 cacttcttca gtcaatctag ctaagttagc taatggtgaa aaatatcctg ttttaaattg
      361 tccaatatat tcatcaactt cacgttgcat ttctaccatt gatttcattt ctacgttctc
      421 cttatattgc atttctaata tagtatatat caatttgaag tctcatgcat gtttagtgca
      481 atctactgac tctagctaaa ctagatgaca agttgtttat tttagaagtt taataattca
      541 gtttatataa atgtaatgca ttcctaacta aattaaatca attgaaaatg ggattataac
      601 tttatgatac gtactactac aataaaataa tatagtgaat aatctaccat tagaaaaata
      661 agcacaaaaa aactagcaac cacacaaaat gtgattagct agttaataag tgtctaattt
      721 aagttaattg ttaatctata agattaatca cttgaacgcg caagcaaaat gatacgtaca
      781 agctctgcta cagcgacagc agttgctgca acataagtca ttgctgctgc agataatact
      841 ttacgcgcat gcttgtattc tttttcattt acaatgttca atgccgtaat ttgtttcatc
      901 gctcttgaac tcgcatcaaa ctcaactggt aacgtaacaa ttgagaataa taccgctaat
      961 gacattaaac cagcaccaat ccataaagca gttgaaccaa atgcactacc tatcgctgtt
     1021 aagataatac ctaacatgat gatcatataa cttaatgaac tccctaggtt tgcaacaggt
     1081 actaatgctg ctctgaatct taagaaccaa tatccttggt gatcttgaat ggcatgacca
     1141 acttcgtggg ctgcaattgc agttccagca actgatggtc tgtcatagtt tgcaggagat
     1201 agtgaaacaa ctttcttttt aggatcgtaa tgatctgtta agaatccttc acctttaaca
     1261 acttcgacat cataaatacc gtttgcatgt aaaatttcta atgcaacttc acgacccgtt
     1321 ttaccactag ttgatctaac ttgtgaatat ttctcatagt tagatttaac tttgtgttgt
     1381 gcccataaag gaagcaccat taatattacg aaataaatta tcatagtaaa aattgaagac
     1441 aataaactca ctctccttta taaatatttt actgtcattt gccgttttta tcaaatcatt
     1501 tacactttaa taatttgttt aattcaatat aaagcaaaag tccaaaaaca ctcagacaac
     1561 atgataatac accaatttgc cacacatgtg tagttataaa atcataatat ggaaattgaa
     1621 ggtgaaaata gtcaatataa tcattcaaaa atacccaagt catcgctaca ctgattccaa
     1681 tcatagaacg tttaaaccta ggatagaagt aaattgcctg aacggccatt atactgtggg
     1741 aaaacattaa tatcaggcca tttactgtaa tatcaccttg ttcaataata aataatatat
     1801 tcataataac tgcccaaatc ccatatttga ataatgttac aaatgccagt gcatcgataa
     1861 tactattttg tttttggatt aatatcattg agatagaaat aactaagaat aatattgcag
     1921 ttgggctatc tggaacaaaa atcttaaaat gccacggcgt atgacttaat tgttcaccat
     1981 accatatata accataaatc atccctaata tattacaaat gagtagcatc attaaccaag
     2041 aacgttgata aagtgtatat tgccaaaatg tattaatcgt catctgctaa gtcctcaaat
     2101 tgattatgtt tatttactag cttgagtgta tttaaaattt gcgttagttg ataaaaacgt
     2161 tgcttttcat tcatctgtaa acttaaatca atattgtgta acaagtaatc tattaataac
     2221 gcatgtttat gccgatctat agccatacta tttaagtcat gaagataagt ttgataactg
     2281 ggcgattccg ttagttgagc aactattaaa tcatctagcc tttgctcacg ctttgacacg
     2341 tttgcgaagt gaatttgaat atcaaaagca cagttatgat tagcgatata atcaaatatt
     2401 tcatttgtat tcattaactt tatattacgc ttagtaaatt gaattgcaga agcgtgactt
     2461 cccacttctg caatttctaa tgtttcatga tgattaattt ttgtatctac aaaatgaatg
     2521 tttgccaatt tcgcctcatt cacttttata tagttaagca cccaaactgc aatacgcgac
     2581 ttaaatcgat attgaaaaag taaatattca ataaaacttt ctttaatttg attgagtgtc
     2641 tctgacatca aataccccat tttaagattg caatcttgat aattcgtcat gccaattttc
     2701 gttacttggc tctagttcca acaattgatt taaaatagta attgcttgtt ccttttgacc
     2761 aatttcagtt aaatagaaat aataatcact cataaaatca atatttgttt tcatcgttgg
     2821 atatgctaat tcaaagaaat gttgagcttc tttatctcgc tcttcttgac cataggcgaa
     2881 cgctaaatgc cacatgaatg taggatccaa atcttcttca tctacatatg ttaataattc
     2941 aatgattgct tcataatctt cttcattacg atataaatcg cttaaaatca ataaaggttc
     3001 ttggtaagca ttatcaacct ctaatgcttg tcttaacaat aatacacctt cattagcatc
     3061 gccgtgttct atttccaaac atccagttgt atacattaac tctttataaa attgacttag
     3121 tcgtaatcct tctttacccg tctcaatggc atctggataa tttttttcat tttcatataa
     3181 tgattgtaaa tacaagtagc cttgaatata atcaggatct ttagaaagta atgtagtcat
     3241 tattttaatt gcttcttgag tgatgtcatt tttatcgtaa gaaatggctt ttttgagata
     3301 atcttctgaa gtcatttcat cttcattaat ttcatcgtat aagcgaatcg catcactata
     3361 gttaccactt tgtaaactac aatctgccat acgagagaat aagtttacac cattaacttg
     3421 atattcacca gtttctaaaa cggtttcgta ttcagaggta gcacgtaaat attgaccatc
     3481 ataatataac atttcagcca atgcaaaatg gattattgga tcatttggct ctagttcaag
     3541 tgcttcttgt aatttagcaa tagcaacttc catcatatta atttgttgat ataaatctgc
     3601 ttctaacatc aacttttcag gtgatggttc aacataactt aaatattcta acgcttcgtc
     3661 agtttgattt tcagacatta aaccttcaat aaaataaatc agcaattcac tttcgtctgg
     3721 atatttgtga tataacacgc gaaatacttc caaaccttgt ggcattaatc caaaattgta
     3781 aagtgtctct cctagaataa ataatgcgtc atcgttgtca gtagttattg cttcattaac
     3841 acgagagtct aaattttcta gtttttgtag attgatatcg tctattaatt tatagatatc
     3901 ttccatttta tattatccct cattttctaa aagctttagt tttggtaaaa atcctggaaa
     3961 tgatacattt acagcatcaa attgtttgat tttgacaggc tcgcttgaaa gtagagaagc
     4021 aactgcaagc atcattccta ttcgatgatc agttaaacta tcaactgttg catttgtttt
     4081 aaattctgac ggatgaataa tcaatccatc attagttggt tgtaattcaa accctaacaa
     4141 gtttaacata tcagccgttg tatcaattct atttgtttct tttactttta attcctcggc
     4201 atctttaatt gtactcgtgc caactgcttg tgtacaaagt aatgctatta caggcagttc
     4261 atcaattgct tttggaacta attctccttc gattgttatt ggttgaagca ttggtgtgta
     4321 ttgaatacga atagaagcag taggttcagc accagttgtt tgattgaaaa gttggatatt
     4381 accgcccatt ttttcaacaa tatcaataat acctgaacgt gttggattga ttccaacatt
     4441 atgaattgtt acatcacttc ctggtgtgat aagtgctgca acaataaaga acgctgcaga
     4501 tgaaatatcg ccaggaacat gaaaatctgc aggtttaatg tatcgaattg cttcaggggt
     4561 tgtattaatt gataaccctt ctgcttcaat tggaatatta aaatgtttga acatcgtctc
     4621 agtatgattt cgacttacat ctaattcttt aatgatggtc ggttccttag aaaacaaact
     4681 tgcaaataaa atggcacttt ttacttgtgc acttgcaact tccatttgat aatttatacc
     4741 ttttatgaca gatggcttaa taattaatgg tgtataatta tcttcaatac cttcaatatt
     4801 cgcatccata agtttcaatg gtctcaagac acgatccatt ggccttttac caattgaaac
     4861 atcgccagac aaaacacttt caatacctaa accacttaac aaacctgcca ataatcgtgt
     4921 tgtcgtacca gaattacctg tatacaatac ttgatgtggc gtgttaaaag attgatatcc
     4981 tggggaagtc acaactaatt tttcatcatc ttctttgatt tctacaccta acagtcggaa
     5041 aatgtccatc gtacgacgac aatcttcgcc aagtagtggc ttatatatag tagatacacc
     5101 ttcagctagc gacgccaaca tgattgcacg gtgtgtcatt gacttatcgc ccggcacttc
     5161 tatttcgccc tttaacggac ctgaaatatc aatgatttgt tcatttacca tttcattcac
     5221 ctacttaaaa tatgttttta attgttcaca tgcatgttgt aatgttagtt gatcaacatg
     5281 ttgtacaacg atatctccaa attgtctaat caagaccatt tgtacacctt gcttatcatt
     5341 ctttttatca cttagcatat attcgtataa cgtttcaaaa tccaagtcag ttatcatgtc
     5401 taaaggataa ccgagttgta ttaaatattg aatataatga ttaatttcat gcttagaatc
     5461 aaacaaagca ttcgcaacta taaattgata gataatgcca accatcactg catgaccatg
     5521 aggtatttta tgatagtatt caacagcatg cccaaatgta tgacctaaat ttaaaaattt
     5581 acgtacacct tgttcttttt catctgcaac aacaatatcc agcttcgttt caataccttt
     5641 agcaatatat ttatccatac catttaatga ctgtaatatc tctctatctt taaagtgctg
     5701 ttcgatttct tgcgtcgttg attcaccatt caataacgca tgcttataaa cttctgcata
     5761 gccacttaat atttgctcaa atggtaacgt ctttaaaaag tctaaatcat aaatcacagc
     5821 agttggacga taaaatgcac cgataaggtt tttaccttgt tttgagttaa tacccacttt
     5881 accgccaaca ctagaatcat gcgccaaaat cgttgtagga acttgtataa aatggacacc
     5941 tcttaatagt gttgctgcta caaatcctgc aaaatcacca gttgcaccac caccaacagc
     6001 aataattgct gtattacgag tcacatgatg ggataaaata tactctaatg tttcttgata
     6061 ttgatgaaat gtttttgtct tttcacctgc tggaataatg actttatgca cattttcata
     6121 tgataaaata tcatcaaatt tattcgcaaa atattgattt acatattcat caattaatat
     6181 aaaactttga tcaaactgat caatatacgt gctaatatgg tcaattgcac cgcgttcaac
     6241 atatattgga taattatttg aagggtatgt tgtttgtaat ttcatgatta cacctcaatt
     6301 gttcttgttg ttaaaactca atatttaatt gtctgcgctc aataatttgt tgtttaagtt
     6361 gctcaatatg atttgattgg aattcttcca ataatgcttt cgcaatttca aatgctacga
     6421 catgttcgca gacgatactt gctgcaggaa cagcacaact atcagaacgt tcaattgttg
     6481 ctttaaagtc ttctttagta ttaatgtcta ctgaatttaa tggtttatat aacgttggaa
     6541 ttggtttcat tacaccatta acgataattg gcattccatt tgacataccg ccttctaaac
     6601 cacctaagtg atttgaccca cgataatagc ccaattcagt attgtagaga atttcgtctt
     6661 gaatttcgct accaggcttt tcagctgctt taaatccttc tccaaaactt acacctttaa
     6721 atgcattaat actaacgaca ccctgtgcta ttcttccatc taatttacga tcataatgta
     6781 cataactacc tacaccaaca ggcatatttt caactacaac ttgaactact ccccctattg
     6841 aatcaccatc gtttttagct tcgtcaattt tatctcgcat tgcttgtgcg atactgtcat
     6901 caattacacg aacatcatta cgatcaagat ttgctttaaa tgtttttgaa tcataaaaat
     6961 ctttatcttt aattccacct atttcaacaa cacgactgta tatatcgata tctaactgtt
     7021 gtaataacac tttacataag gcaccgactg caactcgagc tgctgtttct ctagcagatg
     7081 atcgctctag cacatttcgt aaatcacgat gattatattt catacctcca accaaatctg
     7141 catgaccagg tcttggtttt gtaatagtac gtttcatatt ttcacgttct tcttcactta
     7201 ttggagctgc tcccataatt tttctccaat gcgtaaagtc atcattggtt acaaccatag
     7261 taattggact acctaatgta taaccatttc taacgcctga tactatttct actgtatctt
     7321 tctcaatttg catgcgtcgg ccacgaccat aaccaccttg acgcttgaac atttctttat
     7381 taatatcttc aactttaatt tctaaatttg ctggtatacc ttcgacaata actgttaatt
     7441 gaggtccatg tgattctcca gaagttagat atctcataca cactcgctcc tttcatactt
     7501 ccgcaataac attataatat gttgttaatc ttatcacatt cattgtggat tcataaatgt
     7561 ttgttttcaa gcttaattaa gaaaatggat gacatctcat tttaaatgtc agaattatct
     7621 taatattaaa cgaactaaaa ttaattataa tatattcgtc tgtagaaaag tatcatgaat
     7681 ttctaaacag ttgcatcata taatcattaa ttaaaaatga ttagccgttt taaaataatc
     7741 gtatcgcaac tttagcaaat aatatactga ctacatttct taatacaaaa gaacgaactg
//
LOCUS       DAMZLE010000107         7627 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMZLE010000107 DAMZLE010000000
VERSION     DAMZLE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.108"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..804)
                     /locus_tag="POB92_002070"
     CDS             complement(<1..804)
                     /locus_tag="POB92_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579542.1"
                     /translation="MKKLYKNRSFSNLLMSRILINAGDSLYYVSTTWIVLQWTQNPLL
                     VGLTNTLLMIPMCISFLFGPLVDALPLKKTLFLTPLLQCLLLSLIASLNLIGYLNVYG
                     LIILVAIAMFCTQIGYPAQSKAIPLLVKKDQLLDANSAMSVAYQGTDVIMNSISGIIV
                     AAIAFFPLYSINSLIFLIACLFMLNVNIPNITNPARNNPSYLQNLKEGVLGIKNSLLL
                     VVALVSGVINFGLGLLYTWIPLKADHLGGAYYYGLLMALFSIGLIVGSLI"
     gene            complement(1047..2051)
                     /locus_tag="POB92_002071"
     CDS             complement(1047..2051)
                     /locus_tag="POB92_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6579543.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDFPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            2242..2757
                     /locus_tag="POB92_002072"
     CDS             2242..2757
                     /locus_tag="POB92_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579544.1"
                     /translation="MAGIIKEISKQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            3071..3685
                     /locus_tag="POB92_002073"
     CDS             3071..3685
                     /locus_tag="POB92_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579545.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQANNNQKSS
                     YVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDY
                     SIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(3839..5512)
                     /locus_tag="POB92_002074"
     CDS             complement(3839..5512)
                     /locus_tag="POB92_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6579546.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPN
                     SERADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            5829..7427
                     /locus_tag="POB92_002075"
     CDS             5829..7427
                     /locus_tag="POB92_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6579547.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
BASE COUNT         2675 a         1139 c         1236 g         2577 t
ORIGIN      
        1 gattaatgaa cctacaatta aacctattga aaatagtgcc attaataaac cgtaataata
       61 ggctccacct aaatggtcag cctttaaagg tatccatgta taaagaagtc ctagtccaaa
      121 atttataaca ccacttacca atgcaacaac tagtaggagt gaatttttaa ttcctagcac
      181 accttctttt aaattttgta aataagaagg attatttctt gcagggttag ttatattagg
      241 aatatttaca ttcagcataa ataaacaagc tattagaaat attagtgaat taatagaata
      301 tagtggaaaa aaagctattg ctgcaacaat aataccactt attgaattca taataacatc
      361 tgttccttga tatgcgacac tcatagcaga atttgcatct aataattgat ctttttttac
      421 taataaaggt atagctttag actgagctgg gtatcctatt tgagtgcaaa acattgcaat
      481 tgccactaaa atgatcaagc cgtatacatt taagtatcca attaggttta aagaagcaat
      541 tagtgatagt aatagacatt gtaataaagg ggttaaaaat agagtcttct ttaatggtaa
      601 ggcatctact aaaggaccaa ataaaaatga aatacacata ggaatcatta ataaagtatt
      661 agttaaacca actagtaaag ggttttgcgt ccattgtaaa actatccaag ttgtagaaac
      721 ataatagaga ctatcaccag catttatcaa aattctactc ataagtaaat tagaaaacga
      781 acgattttta tataactttt tcaaaatatc acacctattc attgaaagtt atttacatta
      841 attataataa taaaatttat taaatcaagg tattattgct atttaacaaa caatttactc
      901 tactgtctgt tgtctttgtg taccgtttag ttgaatttta attatttaat tcttttattg
      961 attaatttgt gtaatgttga ttaagtagat aatctaaaag aaaataaaaa gacatctcgc
     1021 ataacgggat gtctttttca tgaatactat ttatctacac cgaaatcgat aacaatgcgc
     1081 cctttatttt catgtttaat tactttgtta agttgttctg gaagttcatc aaatgtaata
     1141 acttgcttga tctcatgtaa attttcaggc attaaatctt ttgcgagacg acgccaaacg
     1201 cgctgtctta atttcatagc agtaaatacc gagtcaattc ctaaaatgtt tacacctctt
     1261 aaaatgtgag ggaatacaga attagtataa gtattaccgg cagtcatacc aataactgca
     1321 attgacccac tatgatttaa acgctttgta acataattaa taccttcgcc accaacaggg
     1381 tctacacaag cttgccaagt tgatgatgcg agtggctttt tatgatcatc ttcaacagga
     1441 agtcgatcga taacttcttt ggcaccaagt tcaagtaatt gatcgctaac atcttgttta
     1501 cctgtacttg cgataacttt ataaccaagt tcattaagca tgagtactgc taaagtaccg
     1561 acaccacctg aagcaccgcg aacgagtaca ggaccatctt caatattcat tccaactttt
     1621 tcaagtcttt caattgctaa accggcagta taaccagctg tgccatatat cattgattct
     1681 tctaatgtta aagtatcagg aagcttgata atccattctg attttacacg cgcatattca
     1741 ctaaaaccgc cataatggct aacacctagg tcataactcg ttacaattac ttgttctcct
     1801 ttttcaaagc ctggtgcttc ggattcaaca attgttccag ctaaatctat tcctggaatc
     1861 ataggatacg attttacgac tgcattatga tcttgagtcg ctaaagcatc tttataattt
     1921 ataccagagt aatgtacttt aatcagcaca tctcctttag gaaaatcagt aggcgataat
     1981 tgtttgaaag ttggtgtcac tttaccgctc tcatctttat caattacaaa tgctttaaat
     2041 gtttctatca caacgataca tcctctcttt ttctcttttg aaaatacggc cattttactg
     2101 gaataaatgt atcatttatt gatgttacac acaatcaata caatctgtaa ttgatttgtg
     2161 ttaatgtatt tttaaatagt tttggctgaa aaataacata agtaatattt tgattcgatc
     2221 actgtttatt ggaggattta tatggctggg ataatcaaag aaatttcaaa acaaaatgct
     2281 agtgaattag ttgaattagc aactagaaca ttttatgaca cgtttggttc ttactatgat
     2341 gacaaagatt ttgatcaatt ttttaaagac aattatactg tagaaaaatt tacacaagag
     2401 attaaccatg tagattcatt tcattatttt tatcaagaag atggtgcgaa tgttggttat
     2461 ataaagatga atattaatag tgctcaaact gaagaaatgg gggagaccta tttagaagtg
     2521 cagcgcatat attttttgaa agactttcaa ggtggcggaa gaggttcaca attgatagaa
     2581 ttggccgaaa aaattgcaca agaacataat aaacataaaa tttggctagg agtttgggaa
     2641 cataatcctc gtgctcaagc attttataaa cgtcatggat ttaaagtggt tggtgaacat
     2701 cattttcaaa caggtgatgt aacggataca gatttaatta tggaaaaaga gttataatga
     2761 ttgtttttgc agcagataaa atgaaatcat agtaataaat attgtgtata ccttgaaatg
     2821 ctaaatgcgt aatgaattgt taaagtaaaa taattaaatt tatagaaaac ataaaattca
     2881 caaaaattaa ataataatga acaaaaatta gggtataata aaaagaacct tagattgtag
     2941 ttatggatat tgtgataatt cagatcatat taatgttgac gtctttggta acaagccatg
     3001 aaaagataca catggtagat atgtatttca agattattca atgaatatcg aattatagga
     3061 ggagatatgt atgaaaagat tagttacagg gttactagca ttatcattat ttttagctgc
     3121 atgtggtcaa gatagtgacc aacaaaaaga cggtaataaa gaaaaagatg ataaagcgaa
     3181 aactgaacaa caagataaaa aaacaaatga ttcatctaaa gataagaaag ataataaaga
     3241 tgatagtaaa gacgtaaaca aagataataa agataatagt gcaaacgata accagcaaca
     3301 atctaattca aatgcaacaa acaatgacca aaaccaaaca aataataacc aagcgaataa
     3361 taatcaaaaa tcaagttacg ttgcaccata ttatggacaa aatgccgcgc cggttgcacg
     3421 tcaaatttat ccgtttaatg gaaataaaac tcaagcttta cagcaattgc caaatttcca
     3481 aacagcttta aatgcggcta ataatgaagc aaataaattt ggtagtaata ataaagtgta
     3541 taatgattat tctattgaag aacataatgg caactataag tatgtgttta gttttaaaga
     3601 cccaaatgca aatggaaaat attcaattgt aacggttgat tatactggac aagcaatggt
     3661 tactgatcca aactaccaac aataatgtta atataactat gatgcaagtt aaaaaaataa
     3721 aacggtaaac tctataatat gaattagggt ttaccgtttt ttgcgtattt taaagtatca
     3781 aagtttacgt tttatagtac ttttaaacct cattcacagc cactgagaaa tttaaacact
     3841 attgattaga tgtcatctta gacaatataa gttgtgaaat agattctaat ggttgttctt
     3901 tatgttgtat ttgtggatat ttaatcactg gtattgcctg ggaaattaaa ttattaatta
     3961 ataattgtcg gtctgtatac caaatttttg atagatgacg ccaaatatct tcgttaacac
     4021 cttgagactc aatgtattga ctgtgatttg gagtgtataa tgctactgtt ttgtctatag
     4081 ccatcgcatc aaatattata tttgagtaat cagtgataac gacatcagac attagaatta
     4141 agtcttgagc actaagatac tttggtgcaa caagcgcctc ttctggtaag atcgcttcat
     4201 caacaccctg aaccacaaca tgatatgctt tgaataaagc atctgatagt ggtaattgtt
     4261 gcgcactcac taatccaata ggtgcgtata ataaaactgg tttttgatca tttattttaa
     4321 acgatttctt atactgttgt tgagtagtac tttcgttacg cttttgtagt aaatattggt
     4381 gtttaggatt accataagcc aatacatttg cttggtgact aggaaaagcc gtttgataaa
     4441 agtggctaat atcatcagca gaatgtataa cataatcgaa ttgcaaccag cgattatacc
     4501 ttttagcacg ataaaaaggt gtttctatat ttttaatagg ctctttacta tctaaaaata
     4561 attgcattat tggtgtgcct tgatgtaatt gaattaaagt accgtttggt tgtaacgttt
     4621 caggtatatc attttcaact ataactacgc gtgcgctgtt aatcaattcg tcagctcttt
     4681 cagaattagg tgatatgaaa tgtggtccgc gacgatcgtc agtaacaaaa taacaggcag
     4741 tcattggatt atgtttaacg aagtaagtaa ataaatattt tgaattacct atatattgat
     4801 gatgatcacc gataaaaacg atgtgatctt ttaaaacgtt tggttgcttc gtatacaggc
     4861 cgaagttttg taattgtttg ttatcagcac gttgctttgc tattttatat aaccatttag
     4921 gtagcataaa cttggtagaa tacaaccaat tatctggcga attaagttgt tctgaatcct
     4981 cgataaaaag gttgcgaaaa ggtataggat gatgatcaat ataagccatt ttattcaaat
     5041 aaaactgaga catcatatca aaatcttcaa tagttttaat agtttcccat attgaagcac
     5101 ttgttgctat aggggcttta aaatcaaaaa cgacatgacc atcgattgat aaatcacctt
     5161 tcagaagtgt caataaagct aaatcgaaaa tcgtttctac agagtaataa tgtattaaat
     5221 caagagtctg ttgaatcgca tatatcaggt taggataata attattgttt tgagcccaat
     5281 cggcaatgtt gatcgatgat tgaccgaaat agtgacaatc atttttgtat tgtccatcca
     5341 cagtataatc aaccacgata gacttaggat ataattgaac cgcatctaac atcgattgat
     5401 aaatcattat ctcatttgca tatggaacga catgtgtata accttcagac tgagctttca
     5461 ttaattgaga ttcaaacttt tgcatattag aaacatttat ctgtttaatc attgctataa
     5521 cttccatttc tcatcgtagt atcttcctta ttttagcacg gtcttatgta aaattcatga
     5581 atagtataag ttacttcatt acattaagta caattcattt tgatgaacag atttaaaatg
     5641 aaaaataaaa caattaaata ggaaagtgat attgttaata gtctgaaaaa atggtatatt
     5701 ttaattgtga aaaaaatcac attaatctca ctgttctgta aaatgttatc gataacaaaa
     5761 tgctatagcc agagcatgat tttaatgaag caattacact actactttat ggaaagaaga
     5821 gtcagcctat gttagtaaat acgtttaacc catttgataa tttattatta tcgtctttaa
     5881 ttgcagcaat accgattgta ctgtttttac tatgtttaac tgtttttaaa atgaaaggta
     5941 tttatgcagc tattacaaca cttgttgtaa cattactaat tgcaatacca tttttcaaat
     6001 tgccagttgg tatcgcttct ggtgcagtag tcgaaggttt cttccaaggt atcattccga
     6061 ttggctatat cgttatgatg gcagtattgt tatacaaaat tactgttgaa tctggacaat
     6121 ttttaacaat tcaagatagt attacaaata tttcacaaga ccaacgtatt caagttttac
     6181 ttattggatt tgcattcaac gcatttttag aaggtgcagc aggatttggt gtaccaattg
     6241 caatttgtgc acttttatta acacaattag gatttaatcc attaaaagct gcgatgttat
     6301 gtttagtcgc aaatgcagcg tctggtgctt ttggtgcgat tggtatccct gtaggtgttg
     6361 tagaaacgtt gaaattacct ggagatgttt cagtattagg tgtttctcaa tcagcaactt
     6421 taacattggc aatcataaat ttcattattc cattcttact tatctttatt attgatggtt
     6481 ttagaggtgt taaagaaaca ttaccagcaa ttttagtagt ttcaatcaca tatacactta
     6541 ctcaaggatt attaactgta ttcagtggac ctgaattggc agatattatt ccaccgttat
     6601 taacaatgtt agcattagca gtattttcta aaaaattcca accaaaacac atttatcgtg
     6661 ttaataaaga tgaagaaatt gaacctgcaa aagcacattc tgcaaaagca gtattacatg
     6721 catggagtcc atttattgta ttaacagtca ttgtaatgat ttggagtgcg ccattcttta
     6781 aaaacttatt cttaccaaat ggtgctttat catcattagt atttaaattc aacttacctg
     6841 gaacaatcag cgaagttacg cataaaccat tagtattgac tttaaatatt attggacaaa
     6901 caggtacagc tattttatta actattatta ttacaatttt aatgtctaaa aaggttaact
     6961 ttaaagatgc tggtagatta ttcggcgtta catttaaaga gttgtggtta ccagttctta
     7021 caatttgttt catcttagca atttctaaaa tcacaactta tggtggttta agtgcagcaa
     7081 tgggtcaagg tatagctaaa gcaggtaatg tcttcccagt tctatcacca atcttaggtt
     7141 ggataggtgt gtttatgaca ggatcagttg taaataacaa ttcattattt gcaccaattc
     7201 aagcttctgt tgcacaacaa attggaacaa gtggttcact tcttgtatct gctaatacag
     7261 ttggtggtgt agcggcaaaa ttgatttcac cacaatcaat tgcaattgca actgcagcag
     7321 taaaacaagt tggtaaggaa tcagaattat taaaaatgac attgaaatac agtgtatgtt
     7381 tactaatatt catctgtatt tggactttca tcttgtcatt attataaaaa aacgtatttc
     7441 aaaatataaa tatacagagg gtgagatgtt ttctaacatc tcatcttttt ttaatggatc
     7501 attaatgaaa gaagtttgac attataataa tggtagcgct ttatgttaaa atgaatagtg
     7561 agtaatcagc aatcaaatta aattggttga tagctgttaa ggtttgtggt tttgtctttg
     7621 tgctatc
//
LOCUS       DAMZLE010000108         7530 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMZLE010000108 DAMZLE010000000
VERSION     DAMZLE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.109"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(94..315)
                     /locus_tag="POB92_002076"
     CDS             complement(94..315)
                     /locus_tag="POB92_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579548.1"
                     /translation="MNHETTQSDWRTVPNCLASQNYISIVKGLVHHFTAIEDEEILDK
                     IYDDFMDDDSITTVLNNDLQTIINHYLLK"
     gene            complement(329..832)
                     /locus_tag="POB92_002077"
     CDS             complement(329..832)
                     /locus_tag="POB92_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDI6579549.1"
                     /translation="MNTIKSTIHTEAIFSDDKQHRYLLKKTWDEKKPACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDKHTDIHLMK
                     TISESDTVILAYGAYAKRPVVIERVSQVMEMLKPHKKKVKKLINPATNEIMHPLNPKA
                     RQKWTLK"
     gene            complement(848..1159)
                     /locus_tag="POB92_002078"
     CDS             complement(848..1159)
                     /locus_tag="POB92_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579550.1"
                     /translation="MKINRYITKGISKQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDENILSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDVFYYVMLLPEE
                     Y"
     gene            complement(1252..1593)
                     /locus_tag="POB92_002079"
     CDS             complement(1252..1593)
                     /locus_tag="POB92_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579551.1"
                     /translation="MKTTTQELKQYITRLFQLSNNETWECEALEEAAENILPTRFVDH
                     TPLAHLTLETYTYYNDELHELSIYPFLMYANNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLQKGGE"
     gene            complement(2059..3684)
                     /locus_tag="POB92_002080"
     CDS             complement(2059..3684)
                     /locus_tag="POB92_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6579552.1"
                     /translation="MDKMKKKLVGGYIRVSTERQVEGYSIEGQITQIEQYCQFNGYEL
                     VDIYADRGISGKSMNRPELQRMLNDAKNGKLDCVMVYKTNRLARNTSDLLTIVEELHR
                     QNVEFFSLSERMEVKNSTGKLMLQILASFSEFERNTILENIYNGQHQRALKGYYQGNL
                     PLGYNNIPDNKKELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGNPFS
                     INSITYILVNPFYIGKIQFAKYKDWNEKRRKGLNDKPIIADGKHPPIISQELWDKVQS
                     RKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNFR
                     NKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVLKQVVERVNQDNQVDVAALNHDIA
                     YKQQQFDEVNIKLKNLVQTIEDNPDLTSALKPTIHQYETKLNDITNQMNQLKQQQNQE
                     KPSYDTKQIAALLQRIFQNIESMDKSQLKALYLTVIDRIDIRKDENHKKQFYVTLKLN
                     NEIIKQLFNNNNLDEVLLSTSSLFLPQTLYFQI"
     gene            complement(3706..5055)
                     /locus_tag="POB92_002081"
     CDS             complement(3706..5055)
                     /locus_tag="POB92_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6579553.1"
                     /translation="MKQAIGYLRQSTTKQQSLAAQKQTIKALAEKHNIQHITFYSDKQ
                     SGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRNLKNALKLMKLCQTYHVHILSVH
                     DGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKARQGKLITTHAPFGYHYH
                     NGTFTIDTVKSPTVKAVFNYYLQGYGYKKIAQYLEADDNFINRKPYQVRNIILNPNYC
                     GRVINQYGQYENMFPAIVSTTIYEEAQVTRTQKQVKCKPSENQLKQKIKCPYCHSTLT
                     NMTVRKPDHSLRYYVCPQNMNNARFVCEFKGINAQELETSVLATCQDFFQNQQLYSKI
                     NHTIQQRLKRQRDIEIKTTLNHEQLIEKLAQGKIDAETFKEQTHSLHQQSKPISSISA
                     YQIRKAFQNIIQQRFTLNMLYPYIDEINISKNKSLAGIYFKNEPLNIVKQTMKLSIV"
     gene            complement(5243..7012)
                     /locus_tag="POB92_002082"
     CDS             complement(5243..7012)
                     /locus_tag="POB92_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017176075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDI6579554.1"
                     /translation="MSHYIIFEAGPYQLDAKAWRKTFPPKKDGDKEKVVPISSCIVVE
                     AKFWHASENTEKIIISNGKDKEHTFESDILTTRNLPSLIKYGHSINESYIRDLSYALQ
                     LMRDELPTSDLYDGSGIIETPHGYIVMVDTVYTSKQYDQLASLEPIVDSAYLLEPKGT
                     FKDWLNMYMNEVKGHLLLELAVIFGISALVTAFLKHKHEVEFAGIIFSFTGQSSTGKS
                     TAAALAVSVAGNPTKGNKTLFRSWNGTRNALEGYLSNNYGIPMVFDELSSATFKDTTG
                     LLYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFTDSAQNDGLRVRTIEINEQ
                     FTTNATNADNIKKAVALNYGHVLPLVAQYLINREEEVIQWFKDEHHWFKAKLKSEANN
                     TGIRMFKRYAVITTSAQILGRVLATNIDIAKIRDYFIDYHTHTVSERSLADKAIEVIT
                     QFVAQNRGKFSDDTALKNMIENYGLIALKDDYIEVKIIATVFKDMLKEQHFQDVNNVV
                     NALRDKGFIESDRDRITTKRTVKDDYGKKQSLVFYHLKLDSEYASIFGLTKDAEPIKA
                     KINLDNKKMLESWKKQNDDLIDF"
     gene            complement(7012..7308)
                     /locus_tag="POB92_002083"
     CDS             complement(7012..7308)
                     /locus_tag="POB92_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6579555.1"
                     /translation="MTYEQEDDFKLLKRRILGIGFVLPEGQVFTFHDLNRRANVQCSK
                     AVQQNVGRWFAYFVKNAPSVPFIIIGKNTNGHLVYMKVGPNPLQDSNPSKGGVR"
BASE COUNT         2133 a         1225 c         1331 g         2841 t
ORIGIN      
        1 ctattatgtt aattaaaaat agaaaaaaat agtaataaga gcatccctca ccataaaagt
       61 gaaggatgct ttagttttat tagaatatag ttttcatttt aatagataat ggttaataat
      121 tgtctgtaaa tcattattaa ggaccgttgt tatagagtca tcatccataa aatcgtcata
      181 gattttatca agtatttctt catcttcaat cgctgtaaaa tgatgtacta gtccttttac
      241 gatagatata taattttgag atgctaagca attaggaacc gttcgccagt ctgattgtgt
      301 agtttcatgg ttcataggta agcctccttt atttcaatgt ccatttttga cgcgctttag
      361 gattgagtgg atgcataatt tcatttgttg ctggatttat gagctttttg actttctttt
      421 tatgaggttt taacatttcc atcacctgtg atacacgttc tataacaact ggtcgcttag
      481 cataggcgcc ataagctaga atcactgtgt cactttcact aatcgtcttc atcaagtgaa
      541 tgtctgtgtg tttgtcataa ggctctttga tatgtttaag gttctctggg gttttaatat
      601 ttgaaaatag attcacaaga tatacagcac catattgttc agaattcgct aattggttaa
      661 gaataagaac agtagtaaga tcgagtgata acacaccatc taaatggggg tacatcgtta
      721 tcactgtaca agctggtttc ttttcatccc aagttttctt gagtaaatag cggtgctgtt
      781 tgtcatcgct aaatatggct tctgtgtgta ttgtactttt aattgtattc atatatcatc
      841 atttccttta gtattcttct ggtaaaagca tcacataata aaaaacgtct acatcatctt
      901 ctcggatgac gtagactttc ttaggtaatg cattttgatt ttttatatag tttgtatagt
      961 gatattccaa tttgtatgcg ggttgttctt gttcatgtgt gattgagagt atattttcat
     1021 cttcttgtag tttaaaaatg tgtaggtaat ctgtattaag ttgattgtct cgatctttta
     1081 ccatattcca aagtaggatt tgaaggtcta gagatagttg tttactaatg ccttttgtga
     1141 tgtatcgatt gattttcata ctattttcct ccattttgct tttctttcat gatgtcaatt
     1201 acttcgttaa taactgtaac agatagttga gcgatcttga tccagttcat attattctcc
     1261 tcctttttgt aatagatgac gttcatcgat aattgtattt ttagtatctg tgaggtatag
     1321 aaagtccata tcaaaatgat ccagataacc aatactgatg agttggttat tggcgtacat
     1381 aagaaatgga tagatactta gctcatgtag ttcatcgtta tagtaggtat aagtttcaag
     1441 tgtaagatgc gcaagtgggg tatggtctac aaagcgagta ggaagtatat tttctgctgc
     1501 ttcttctaac gcttcacatt cccatgtttc attgttagat agttggaata gacgagttat
     1561 atattgtttg agttcttgag tggttgtttt catatcattg cctcctagat agtgttagcg
     1621 atgtatttca tatacatcac tgagataata tatatttgat ttatcattta ttacgaatcc
     1681 cggtgggaat aagagaaaat cccatatgaa aaactgctac aaacattgat atgacaagaa
     1741 aatcccgtta tcccgctcat ttgttgggaa atataggtat atatttatat attatttgga
     1801 tttggtggtg ttattacgac ttactttttc tttttatatt atttatttat aaataataac
     1861 gggattttgg gattacgctt gcgtaatcct tctcctacat gaagttagcc aatcccgtta
     1921 ctatcccact tatattttgt tttgggatgt tctttcgtca tatcaatata agcttcacat
     1981 tgagccatga tttgttagcg tgttgaatat atttgttaga tgagtgttat tgaatgacga
     2041 aaggacagta catagatatt atatttgaaa gtagagtgtt tgaggcaaaa acaaagacga
     2101 agtgctgagg agcacttcgt ctagattgtt attattaaaa agttgtttaa taatttcatt
     2161 attgagcttg agagtgacat agaattgttt tttatgattc tcgtctttac gaatatcaat
     2221 acggtcaata accgttaggt ataatgcttt gagctgtgat ttatccatgg attctatatt
     2281 ttgaaatatt cgttgtaata gggcagcgat ttgtttcgta tcataagatg gtttctcttg
     2341 attttgttgc tgcttgagtt gattcatttg gtttgtaata tcattcagtt ttgtttcata
     2401 ttgatgaatg gttggtttga gtgcagatgt taagtctgga ttgtcttcga tggtttgaac
     2461 gagattttta agtttgatgt ttacttcatc aaattgttgt tgtttataag caatatcatg
     2521 attgagtgca gctacatcga cttgattatc ttgattaaca cgttcgacaa cttgtttgag
     2581 aactttatcg cttttgacaa tttcaagtat ttgatccata acgtatttct caatgacatc
     2641 ggctctaacg ctattcgcag aacatacttt tgagcctttg tttcggaagt tactacaaga
     2701 ataataacgt attcgcttct tagtaccatc tttcaatgtg ttcgttgtgt tactagctgc
     2761 cattggtgca ccacattgtg ggcagtgaat aatacctgtt aatagattgg ttcctttccc
     2821 atgaacttga ggtttttgac tgacctgttt tttgcgtgat tggactttat cccataattc
     2881 ttgactaata ataggagggt gtttaccatc agctataata ggtttatcat ttagtccttt
     2941 acgacgcttt tcattccaat ctttgtactt cgcaaattga attttaccaa tgtagaatgg
     3001 attgactaag atataggtta ttgaattgat actaaatgga ttgccttttt tagtcacata
     3061 gcctttgtga ttgagtgcat tggctatttt acgataacca tgacctttgg cataagattc
     3121 aaagatatat ttaacgatgt tagcttcatg ttgattaatc attaattctt ttttattatc
     3181 aggtatgtta ttatatccta atggaagatt gccttgataa tagcccttta aagctctttg
     3241 atgttgcccg ttgtaaatat tctctaaaat tgtatttctt tcaaattcgg aaaaacttgc
     3301 aagtatctgg agcattaact tgcctgttga atttttgact tccatacgtt cagacaagct
     3361 aaaaaattca acattttgac gatgaagttc ttcgacgatt gtaagtaaat cggaagtatt
     3421 acgtgccaaa cgatttgttt tataaaccat aacacagtct aattttccat ttttagcatc
     3481 atttaacatg cgctgtaatt caggacggtt catagatttt cctgatatac cccgatctgc
     3541 atatatatca acgagttcat agccgttgaa ttggcaatat tgctctattt gtgtaatttg
     3601 tccctcgatg ctataaccct ctacttgtct ctctgtggac acgcgaatgt agcctcctac
     3661 aagctttttc ttcattttat ccattataat ccatcctttc tttaattata caattgataa
     3721 tttcatagtc tgctttacaa tattcagtgg ttcgtttttg aaatagatgc cagcgaggct
     3781 tttattttta gaaatattaa tttcatcaat ataggggtac aacatgttta gcgtgaaacg
     3841 ttgttgaatg atgttttgga aagcttttcg aatttgatac gcactgattg atgatatagg
     3901 ttttgattgt tgatgtaatg agtgcgtttg ttctttgaac gtttctgcat caattttgcc
     3961 ttgggctaat ttttctatca gctgctcgtg attgagtgta gttttaattt ctatatctct
     4021 ttgtcttttg agtcgttgtt gaatagtatg gtttattttt gaatagagct gttgattttg
     4081 aaagaagtcc tgacaagtcg ctaaaacact tgtttctaat tcttgtgcgt tgattccttt
     4141 aaattcacaa acaaagcgag cgttattcat attttgcgga cagacatagt aacgcaatga
     4201 atgatctggt tttcggacag tcatgtttgt gagtgtcgaa tgacaatagg gacatttgat
     4261 tttttgtttg agttgatttt ctgacggctt acattttact tgtttctgag ttcgggtaac
     4321 ttgagcttct tcgtatatcg ttgtactgac aatagctggg aacatgtttt catattgtcc
     4381 gtattgattg ataacacggc cacagtaatt aggattgagg ataatattac gcacttgata
     4441 aggcttgcga ttaatgaagt tatcatcagc ttctaagtat tgcgcaattt ttttataacc
     4501 ataaccttga aggtaataat tgaacacagc ttttactgtt ggtgacttta ctgtgtctat
     4561 cgtgaaagta ccattatgat agtgataccc aaagggcgca tgtgttgtaa ttagtttacc
     4621 ttgtctcgct ttttctttga ttccattttt gacttgttcg cctatattat cagattctag
     4681 ttcggccaag ctgatgaaaa tattgagttt gagccgatcg aatgctttat ccatgtcgaa
     4741 gtagccatca tgaacgctta agatatgaac atgatacgtt tgacataatt tcatgagctt
     4801 taatgcattt tttagattac gatgaagtcg atttagacga tagcaacata atacgtcaca
     4861 ttgtccttgt tgaatcagtt cagtaatttg ttgataacca ttccgcttat cagtacgtcc
     4921 tgattgttta tcgctataaa aggtaatgtg ttgaatatta tgtttttcgg ctaatgcctt
     4981 gatggtttgt ttttgtgctg ctaaggattg ttgctttgta gtgctctgtc gtaagtaacc
     5041 tattgcttgt ttcatagtat ttcctccttc caaagtgata atatatattt atgaacgaat
     5101 ttatagatga gcccaacacc tgctggtgtt gggcgttatt attagtcatc agcatgatta
     5161 atttcttcaa caactaaatc agctagtaat tcaatcaatt catccatttt atctaatcct
     5221 gtacgattgc atctttaagt tattaaaaat caattaaatc atcattctgt tttttccatg
     5281 attcaagcat ctttttgtta tccaagttaa ttttagcttt aataggttca gcatctttag
     5341 ttagaccaaa aatagacgca tattctgaat ctaactttaa atggtaaaaa acaagtgact
     5401 gtttcttgcc atagtcatct tttactgttc tttttgtcgt gattcgatca cggtcagatt
     5461 cgataaatcc tttatctctt agtgcattca cgacattgtt gacatcctga aagtgttgtt
     5521 ctttcagcat atctttaaat acagtcgcaa tgattttgac ctcgatataa tcatctttta
     5581 aggcaattag tccataattc tcaatcatat tctttaacgc tgtgtcatca gaaaacttac
     5641 cacggttttg tgctacaaat tgtgtaatga cttcaatcgc tttatctgcc agtgatcgtt
     5701 cagagactgt atgagtatga taatcaataa agtagtctct tattttagcg atatcaatat
     5761 ttgtagctaa aacacgacct aatatttgcg cagatgttgt aatgactgca taacgcttaa
     5821 acatacgaat acctgtattg tttgcttcac tcttcaattt agctttaaac caatgatgtt
     5881 catctttaaa ccattgaata acttcctctt cacgatttat aaggtattga gctactaacg
     5941 gtaaaacatg accatagttt agcgccacag cttttttaat attgtcagca ttggtcgcat
     6001 ttgtagtaaa ttgctcatta atttcaatgg ttcttacacg taacccatcg ttttgagctg
     6061 aatcagtaaa aatactgtat tctgacgttg aaatgacaga agttccccaa ttcttaggtg
     6121 ttttaacttc tccatgaacg tttgaacgtt gacgcccttg accttcagcg atggagtaca
     6181 ataaccctgt tgtatcttta aaagttgctg aggagagctc gtcaaatacc ataggtatgc
     6241 cataattgtt actcaagtaa ccttcaagag cgttacgtgt gccattccaa cttcgaaaaa
     6301 gcgttttatt acctttggtt ggatttccag cgacggatac cgctaaagct gccgctgttg
     6361 atttaccggt tgatgactgg cctgtaaaac taaagatgat accagcaaat tcaacctcat
     6421 gtttatgctt cagaaaggct gtcactaagg cagaaattcc aaatatgacc gctaactcta
     6481 gaagaagatg acctttgact tcgttcatat acatatttaa ccaatcttta aatgtccctt
     6541 taggctctaa tagataagca ctatcgacaa taggctctaa agatgctaac tgatcatatt
     6601 gtttagatgt gtaaacggta tctaccatta caatgtaacc gtggggtgtt tctatgatgc
     6661 ccgagccatc atataaatca gaagtgggca gttcatcgcg cattaactgt agtgcatagc
     6721 tcaaatctct tatataactt tcattgatac tgtgaccata tttaatcaag gaaggtaagt
     6781 ttcgagttgt taaaatatca gattcaaatg tatgttcttt atccttaccg ttagaaataa
     6841 ttattttttc tgtattttct gaggcatgcc aaaatttagc ttcaacaacg atacaactag
     6901 atataggcac tactttttcc ttatctccat cttttttagg tggaaaagtt ttacgccacg
     6961 cctttgcatc taattggtat ggacctgctt caaaaatgat gtaatgactc attagcgaac
     7021 acctcctttc gaagggttgc tatcttggag tggattaggt ccgactttca tatatactaa
     7081 atgaccattg gtatttttac cgataataat aaatggaaca cttggcgcat ttttaacaaa
     7141 atatgcgaac caacgtccaa cattttgttg tacagctttt gagcattgta catttgcacg
     7201 acggttcaag tcatggaaag taaatacttg tccctccggt aaaacgaatc caattcctaa
     7261 aattcttctt ttcaacagtt taaaatcatc ttcttgctcg taagtcattt ggatcatcct
     7321 ttgtatatta gtcatcattt aaatgatgcc tttgtatctt atatctacac tgagatataa
     7381 agttctaaat ttgagaggaa aatattagga cattggtatt acaccttttg ttaattaaaa
     7441 aacccaacga taaaaaaatc gttgggttta aaaaggtatg ttaataatat aatttagatg
     7501 cactattcaa tttttttaag cgtaatttat
//
LOCUS       DAMZLE010000109         7372 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMZLE010000109 DAMZLE010000000
VERSION     DAMZLE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.110"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(137..1498)
                     /locus_tag="POB92_002084"
     CDS             complement(137..1498)
                     /locus_tag="POB92_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6579556.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1846..2967
                     /locus_tag="POB92_002085"
     CDS             1846..2967
                     /locus_tag="POB92_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDI6579557.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            3016..4284
                     /gene="icd"
                     /locus_tag="POB92_002086"
     CDS             3016..4284
                     /gene="icd"
                     /locus_tag="POB92_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDI6579558.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVEKEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4674..5378
                     /locus_tag="POB92_002087"
     CDS             4674..5378
                     /locus_tag="POB92_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6579559.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEVDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5378..7039
                     /locus_tag="POB92_002088"
     CDS             5378..7039
                     /locus_tag="POB92_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDI6579560.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDF
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2675 a         1037 c         1366 g         2294 t
ORIGIN      
        1 tgtatttctg aaatgataca ttattaaata gatgagaaag taaaagtttt gagccaagta
       61 cgcaatttaa tattataagt tgcatataaa aacccctgtt gaactttagt atattcaaca
      121 ggggtaaaac ttgttgttat ttatttgatt tagcagcgat acgtttatat cttaagtaca
      181 taaatgctaa aagtataaac caaatcggaa taaaataaat tgcacgtctt gtatcaacat
      241 taataaataa taacccgaac acaaaaatga agaatacaaa tattaagtag cccatatatt
      301 taccacctaa tagtttgtac gtagcatttt tatgtagatc tgggttttta cgactataat
      361 tgatatatgc aatgataatc agaccccata caactaaaaa taacactgta gagatggtag
      421 tcacatacgt aaatactttt gtcgcatctg ggaaaatata gtttagtaat gctgccacaa
      481 gtaataatgc tgatgaagca aagattgcaa catgtggaac gccatattta ttcgtcttag
      541 aaaagttcgg aggtgcttgt tgttgacttg ataaaccgaa aagcatacgg ctatttgaga
      601 atataccact attacatgat gaagcagcag cggttaatac tacaaaatta atcaagcccg
      661 cagcaaacgg aataccgatc aatgcgaata attttacgaa tggactgtta tcaggatcaa
      721 cttgctgcca agggataata gacatgataa ccgctaacgc cccaacgtaa aatattaaaa
      781 ttctaatggg tacactgtta attgcttttg gaattgtttt aactggatct tttgtttccc
      841 cagctgtaac accaatcatt tcaattccta caaatgagaa tagtgccatt tggaaagaca
      901 taaagaatcc agaagcacct ttagcgaata tgccgtgttc atataaattt gtgaaactgg
      961 catgcccgaa ttgagtttta aatgcaaata gaatcatgac gaaaccaact actattaaac
     1021 caataattgt cgctatttta atgatagaga accaaaattc taattctccg aaaagtcttg
     1081 cgctaagtag gttgaatgac attaataaca gtacacaaaa tagtgcactt atccagtttg
     1141 gaatttctgg gaaccaaaag ctaacatatt ttgccacagc cgttacttca gccatacctg
     1201 taataatcca acagaaccag tatgtccatc cggtaacaaa tcctgcaaaa ggcccaatat
     1261 atgtattggt tacatctgcg aaagatttaa attcagtatt ctgtataatg atttctccta
     1321 aacctcgcat aaacataaat aacataaatc ctataatgat gtatgttaat agaattgaag
     1381 ggccggttaa tgcaatcgtt tgaccagcac ctaagaataa gcctgtacca attgcaccgc
     1441 caattgcaat taattgtatg tggcgattgc tcagttccct ttgtaatttt tcagccataa
     1501 tacatctccc ttaaatatag atatgtttat tatgcactta tattgagata tatacaatta
     1561 ttttcggtaa aaatgtgtaa aattccatgt taatatactt tggtttttat aatcatatat
     1621 aataaccaat tgaaaattta attctattgt aaaattcatg gattattcac atcttgaaaa
     1681 agctttaatg gtgctatttg tggctattct gtgacattta catagattta caaaaaaatt
     1741 gttgcacata taatgccagt ttttatattt cacaaacgaa atgcgtttag ctataatatt
     1801 agttgaaagc catttcataa agaaacagta aaggggaaat ttatcatggc agaattacaa
     1861 agaggtttag aaggggttat cgcagcggag actaaaataa gttcaattat tgaaagtcaa
     1921 ttgacttatg ccggctatga tattgatgat ctagctgaaa atgcgcaatt tgaagaagtt
     1981 attttcctat tatggaacta tagattgcca aacgaagaag agcttgctca tctaaaaggg
     2041 aaattaaatc aatacatgac attaaatcct cgtgtgtata cacattttga ggagtatgtt
     2101 acagatcacg tgcatccaat gacagcatta cgtacgtcat tatcatatat tgcacatttt
     2161 gatcctgatg ctgaaaatga atcagatgaa aatcgttatg aaagagcaat gcgtatacag
     2221 gctaaagtag catcattagt tacagcgttt gctcgagtaa gacaagataa agaaccactt
     2281 aagcctaatc ctgacttaag ttatgcggca aacttcctat atatgttacg tggggaatta
     2341 ccaacagata tagaagtaga agccttcaat aaagcactta ttttacacgc tgatcatgag
     2401 ttgaacgcat ctgcatttac agcacgttgt gcggtatcat cattgtcaga tatgtactca
     2461 ggtattgtag cagctgtagg ttctctgaaa gggccattac atggtggtgc aaacgaacaa
     2521 gttatgacga tgttatctga gattgggtca attgaaaatg ttgatgctta cttagatgaa
     2581 aaatttgcta ataaagataa agtaatgggc ttcggtcatc gtgtatataa agatggtgat
     2641 cctagagcga aatatttaag agaaatgagc cgtcaaatta cgaaagacgc tggtcgtgaa
     2701 gaattatttg aaatgtcagt gaaaatggaa aaacgtatgg cagaagaaaa aggattaatt
     2761 cctaatgttg atttttatag tgcgagtgtt tatcactgta tggaaatacc tcatgactta
     2821 ttcacgccaa tctttgctgt aagtcgttct gcaggatgga ttgctcatat tttagaacaa
     2881 tataaagata atagaattat gcgtcctaga gcgaaatata ttggcgaaac gaatcgtaag
     2941 tatatcccgc ttgaagaaag aaaataatca atacaaatta aaaatgaaga tgtaaaattt
     3001 ggaggtaaaa taactatgac tgcagaaaaa attactcaag gaactgaagg attaaacgta
     3061 cctaatgaac caattatccc atttattatc ggtgatggaa ttggaccgga tatttggaag
     3121 gcagcaagcc gagttataga tgctgctgtt gagaaagcct ataatggcga aaaacgcatt
     3181 gaatggaaag aagtgctagc tggccaaaaa gcatttgata caactggtga atggttacct
     3241 caagaaacac ttgatacaat taaagaatat ttaattgctg ttaaaggacc tttaacaaca
     3301 ccaattggtg gtggtattag atcattaaat gtggctttac gccaagaatt agatttattt
     3361 acttgtttaa gaccggtacg ttggtttaaa ggagtaccat cacctgttaa acgtccacaa
     3421 gatgttgata tggttatttt ccgtgaaaat actgaagaca tttatgctgg tattgaattt
     3481 aaagaaggta caacagaagt taaaaaggta attgacttct tacaaaacga aatgggtgcg
     3541 acaaatattc gattcccaga aacttcaggt attggtatta aaccagtttc taaagaagga
     3601 actgagcgat tagttagagc agctatacaa tatgctatcg ataataaccg taaatcagtt
     3661 actttagttc ataaaggtaa tattatgaaa tttacagaag gctcatttaa gcagtggggt
     3721 tacgatttag cattatctga atttggtgat caagtattca cttggcaaca atatgacgaa
     3781 attgttgaaa aagaaggcag agatgctgct aatgctgctc aagaaaaagc tgaaaaagaa
     3841 ggcaagatta tcattaaaga ttctattgct gacattttct tacaacaaat tttaactcgt
     3901 ccagctgagc atgatgttgt agcaactatg aacttgaatg gtgactatat ttcagatgct
     3961 ttagctgcac aagttggtgg tattggtatt gcgccaggtg caaacattaa ttatgaaaca
     4021 ggtcatgcta tttttgaagc aacacatggt acagctccaa aatatgcagg tttaaataaa
     4081 gtgaatccat cttcagtaat tttaagttct gtattaatgt tagaacattt aggatggcaa
     4141 gaagcggcag ataagattac agattcaatt gaagatacaa ttgcttcaaa agttgttact
     4201 tatgactttg cccgtttaat ggatggtgct gaagaagttt ctacatcagc atttgcagat
     4261 gaattgatta aaaatttaaa ataagcaaaa taaaaatagg tgagtctagc aactttaatt
     4321 gctaggttcg cctttttaaa tgaattgaag ttggcaacag gatagaaata ataaagagta
     4381 actaatgatt gattatatag tgtttcttac acattagaca aagcgattgt gtacataagc
     4441 ttaggaataa atgagtaaaa ctcatgcata ggtagtggca tagaaatgat attttcaaaa
     4501 agtttatttc gttgtcccag agtaagtcat gcaaatgata gaaggaaata taagaagtat
     4561 tttatttata tgtaaagtaa tgtaaaaaat gtgtaaagat aatgtgtagg atgttatttt
     4621 tataaatagt tcggttataa tgtaaatata attataagtt agggaggcat accatgtcgc
     4681 aaaaagtgtt ggtagtagat gacgaacatt caatcgtaac attacttaaa tataacttag
     4741 aaacagctgg ttatgaagtt gttgtcgcat ttgatggtga tgaggcttta gaaaaggtag
     4801 aaagtgaaca gccagattta attattttag atgttatgct acctaaaaaa gatggcattg
     4861 acgtatgtaa gactgtaaga acaaataaaa atttagtccc tatattaatg ttaacggcga
     4921 aaaatgatga atttgatcgg gtattaggtt tagaattagg tgccgatgac tatatgacta
     4981 agcctttttc acctagagaa gttgttgccc gtgtgaaagc cattttaaga cgttctcaat
     5041 ttgtaaaaga agtagaagag gttgatgatg atattaccat cggatcgata cgtattagac
     5101 ccgattattt tgaagtatat aagcataatg agttattgga gctaacaccg aaagaatttg
     5161 aactcttgct ttatttgata gaaagacaag gcagagtcat tacgagagaa catatgttaa
     5221 atacggtatg gaattatgaa tttgcaggag actcaagaat tgtcgatgta catatcagtc
     5281 atttaagaga taaattagaa gataatccta agaagccaca acttattaaa actgtgcgtg
     5341 gcttaggcta taaattggaa agacctaaag aacaatgatg aagtttcacc accgcttaat
     5401 gttattgata agcagtatat taattatcag ttttgttaca ttaggcatta ttattcacgc
     5461 ttccatcaca tatgttttat cagaaaaaca cgaacaagct ctagcaacag atgcaagatc
     5521 atatatttat ttagtccaag ataatgagat tgataaagtt aaagaaattg taaaacagca
     5581 aaatatagat ttattgataa ctaagcatga taaaaaggtg tttagtagtg gcaaactgaa
     5641 agctatccca aatagtgata gctatattca aaaaagggac ttttttattt ttaataagaa
     5701 ggttaacggt tatcatgtat ggattaaagg ttacaataac gatattacag aaatgcattg
     5761 gacgttgtgg aaatatctta ttttgacatg tttagttgta ctcatttgtc tgtattttgc
     5821 aagccgtagt tttaagcgta cattaatccg tcccattcaa gaagttactt atgcaacaca
     5881 attgttggca aatggttatt atcacatacg tgtacctgaa agtaatgttg tagaaactaa
     5941 agcgcttttt gtgtctacca atgatttagc acgacgcctt caaaagctta ataatgagca
     6001 aaaaattcaa tccaaccgtt taaaaacaac gattgaaaat attccaagtg ctattttaat
     6061 gatagatcga aatggaaaaa tcgttgttgc aaataaagcg tattatgaac agttcaacat
     6121 ctcgcataat attgaacaag taggctatca cggttatgtt aatactgaaa ttgagcaatt
     6181 aattttagaa agttttaaag ttgaaaaacc tatttatgaa caattggaag ttgcaataaa
     6241 ccaagtccat gccaagtact ttgatatttc atgcgttccg attttaacta ggtctcaaaa
     6301 aagtttgcaa gggattttag ttgtgatgca tgacataaca aatctgaaac aacttgaaaa
     6361 tctaagacgt gagtttgtag caaatgtttc acatgaatta aaaacaccta ttacttcaat
     6421 taaggggttt gctgaaacgc ttattgatgg tgcaaaaaat gatgcggaat cattagacat
     6481 gtttttaaac ataattttaa aagaatcaaa tcgaatagaa tctttagtaa cagatctgtt
     6541 agatttatcg catattgagc aacatacaga gttagataca gactatatga atttatcaga
     6601 tttaacgcgg cgtattattg ataatatgat gactcaagcc aatcaaaaaa atatttccat
     6661 tcatactgat attgaaaaag atgtcattgt taaagcacaa gaaagtaaaa ttgctcaagt
     6721 tatcacgaat ttgttaacga atgcaattaa ctattcttat gaagatggag atattaacgt
     6781 tcgtgtgtat cgagatgact ttcgtgtcat tttcgaagta caagattttg gtataggtat
     6841 taaattagaa gaccaacaac gtatttttga aagattttat agagtagata aagcacgaag
     6901 tagagattct ggtgggacag gtcttggatt atcaattaca aagcatatag tagaggccca
     6961 ccaaggcaat attgaagtga atagtcaagt tggcaaaggc tcaacgttca aagttattct
     7021 aaaagattat aaagaataaa aattaaagtg gtaacagcgc gtgtattttt cacgaagttg
     7081 ttgctgcttt tttatttcgt tcaaatctct attggtatta aattagagtt aatgaagtgg
     7141 aataaattcg gttgtcaatt ttgtcatttg tatatgtcga tttgtaagtt ataagtaata
     7201 gattgttgtg aatcaagcaa ttttataata tatttattat aataaagaaa ttttagtaac
     7261 gcgcttaaaa tagatgctta attaaaaagt aataagtttt atttgaacgg aaataaatct
     7321 taatttaaac acttgaatag acaagtgtaa tcattcgtca tagaaaaaga cg
//
LOCUS       DAMZLE010000110         7299 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMZLE010000110 DAMZLE010000000
VERSION     DAMZLE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.111"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            104..1024
                     /gene="rnz"
                     /locus_tag="POB92_002089"
     CDS             104..1024
                     /gene="rnz"
                     /locus_tag="POB92_002089"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDI6579561.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGVPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKV"
     gene            complement(1127..1942)
                     /gene="proC"
                     /locus_tag="POB92_002090"
     CDS             complement(1127..1942)
                     /gene="proC"
                     /locus_tag="POB92_002090"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI6579562.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            2084..2839
                     /locus_tag="POB92_002091"
     CDS             2084..2839
                     /locus_tag="POB92_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6579563.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKRMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            2855..3103
                     /locus_tag="POB92_002092"
     CDS             2855..3103
                     /locus_tag="POB92_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579564.1"
                     /translation="MNCQSESVVRFCVRYAEQLSVFEEFTVLDILGDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(3319..4227)
                     /locus_tag="POB92_002093"
     CDS             complement(3319..4227)
                     /locus_tag="POB92_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6579565.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIVIGTKVGNRLTKDGSTKWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            4309..4851
                     /locus_tag="POB92_002094"
     CDS             4309..4851
                     /locus_tag="POB92_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDI6579566.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            4956..5405
                     /locus_tag="POB92_002095"
     CDS             4956..5405
                     /locus_tag="POB92_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI6579567.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            5454..6341
                     /gene="xerD"
                     /locus_tag="POB92_002096"
     CDS             5454..6341
                     /gene="xerD"
                     /locus_tag="POB92_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI6579568.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6419..6925)
                     /locus_tag="POB92_002097"
     CDS             complement(6419..6925)
                     /locus_tag="POB92_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDI6579569.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPDFERIIKANYPDYDYNQETSTDPFIVDHHMRRDRSEVISAKEEAI
                     QLRKHRRN"
     gene            7017..>7299
                     /locus_tag="POB92_002098"
     CDS             7017..>7299
                     /locus_tag="POB92_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDI6579570.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRL"
BASE COUNT         2506 a         1083 c         1306 g         2404 t
ORIGIN      
        1 ctagcaaaga cgaattttta acagattttg attcaagtat aaattaaaac taaattgata
       61 caaattttat gataaaatga attgaagaaa aggaggggca tatatggaag ttacattttt
      121 tggaacgagt gcaggtttgc ctacaaaaga gagaaataca caagcaatcg ccttaaattt
      181 agaaccatat tccaattcca tatggctttt cgacgttggt gaaggtacac agcaccaaat
      241 tttacatcat gcaattaaat taggaaaagt gacacatata tttattactc atatgcatgg
      301 cgatcatatt tttggtttgc caggattact ttctagtcgt tcttttcagg gcggtgaaca
      361 gaagccgctt acattggttg gaccaaaagg aattaaagca tatgtggaaa tgtctatgaa
      421 tttatcagag tcacatttaa actatccaat tacatatatt gaaatcgatg atcatctgac
      481 atatcatcat gatggtttta ctgtagaggc gcatttactt aatcatggtg ttccatcata
      541 tggatataga gtaatggcac ctgaaactac aggtacaatc aatgttgaag cgttaaaaaa
      601 cattggtctt gaaccaggtc ctaaatatca agaagtaaaa tctcatgata catttgaaca
      661 taatggtcaa gtttatcaat caaaagattt tagaggtgag tccaaacagg gtccagttgt
      721 agcaattttt ggtgatacaa aaccatgttc taatgaacgt gtgatttcta gagatgctga
      781 tgtgatggta catgaagcga cctatataga tggtgaaaag catttagcca acaattatca
      841 tcatagccat attgaagacg tatttgcatt gataaaagaa gccaatgtta aacgtacgtt
      901 gattacacat ttgagcaatc gctataatac agaagatata aatgaaattt atcaaacatt
      961 gatacaaaat gaagatacac ctaattttaa ttttgtaaaa gattttgata gttttaaagt
     1021 ctaatgacaa agtaataaac aatgcttgcc aaatagtaat aatttataaa aataacccgt
     1081 ccaactatgt atgcgatgat gcatacatgt gttggcgggt ttgtttttat tggtcttcta
     1141 cattagaaag ttcaatacta cggtcgacgg cagcatttag acaatcttcg aaaatagata
     1201 ctaaatcata ttgtgacaat gtatcaaggc cagcttgtgt cgtaccacct ttagaggtaa
     1261 tattttttct taattgagcc atgctcaaat ctgaacgttc aatcatctta cttgtaccta
     1321 taataaggtt gcgtatagat tcttcaactt gttctttttc tagaccaagt ttcgtaccag
     1381 ctttaacgta ttgctcgaat acatgatata aaaatgctgg gccgcttccg gtgatagctg
     1441 ttacttgatg taaatgatct tctgatactt caattacaga accaaatgct ttaactaaat
     1501 cgttaatttc atctttagat ttagggtcaa agttgtttga aaaactaatg ccagtaacag
     1561 agtgtccgac ttgcgcattt gtgtttggca taattctagc aactggattt tggcattcta
     1621 attgttgttt aatataatca atcggaatac ctgccataat tgaaatgaag caattgtctt
     1681 ttgtgatatg tggtttgatg cgtgttgcta gagcatcaaa gtcatgtggt ttggtaccta
     1741 aaaaaacata atctgcatct tttaataatg tcgcatcatc ataactatag ttaacaccta
     1801 gtttttcagc gaatgctttt aaagcttgtt cattagattt atttgttaaa tatatatcat
     1861 tggcatctaa gttgcttgag ttaataattc ctgtaaatat agcttgtgcc atattaccag
     1921 ctccataaaa tacgagtttc attaacgtca catcccttta gtttttgatt tgtatgttat
     1981 attttcttat tattgtatat atattaggaa agataatcaa cttgtctcaa ttatgtaaag
     2041 ttgataaatt gcattctata taattgaaaa ggaaggttaa ttgatgaaag gacaacattt
     2101 tatagtgact ggtggtacga gtggtttagg tttatcgatt gtccgaaagt tactagaaaa
     2161 taaagtgcat gtaacgttgt tggtgcgtga tgttgataaa gcaactagaa tctttgaaca
     2221 ggaattgggg aaaactataa atgttattcc ttgtgactta aatgatttga agtcgataca
     2281 ggcactgcaa tttgaagaca atacatcatt cgatggattc atttatagtg caggtttagg
     2341 ttatttcaaa tctatttcag accatagttt ttcagaaatg attgaaacgt atcagcttaa
     2401 tttaattagt tttaatgttt tatatactgt gttgagacca tatttaacat ctaacgcaca
     2461 tattgtaggt atttcaagtc aagctgcatt ttccacacaa gcaaatgctg cacattatgg
     2521 ggcatcgaaa gcagggtttt atgcattaat gaatgcttta agactagagt caccaaactt
     2581 acatatcatg acagttaatg ttggcccgat tgatacacct tttcatcaaa aagccgaccc
     2641 atcaatgaag tatgctaaaa ggatggggaa aattatgctt gatgctaatc agcttgcaga
     2701 agatatcatt tatggcatta aaacaaaaca attggaaatt aatcggccta aatggatgca
     2761 tcatgcgttg aaaatgtatc aaattgcgcc tagatttttt gaacgatgtt tccctaaatt
     2821 atttaaaaat aaagcataaa taggtgggat ggcaatgaac tgtcaatcgg agagtgtggt
     2881 aagattttgt gtacgatatg cggaacaact atcagtgttt gaagaattta cagtgctgga
     2941 tattttaggc gatatttctg tagaccaaat ttcagacagt acattatatt atacttgtga
     3001 aaaattcaaa ctacttgtgt tgcaaggaaa tgtgctgggt gtacaaataa taactaataa
     3061 tgatgaatca acatgcgaag tgaaatatag aaagatgttt taataaaatt attaagagat
     3121 gaagtggcag aagtttgctt aaggcaatgc cgcaaggcga aattactaaa tactgatgcg
     3181 tgaggacaac gctttaattt ctatcataag tgagtaatca taatcataaa ttatagcgaa
     3241 ttgtacataa acaacagcag taaggcattt ttataatgaa acgcctcatg ctgctgtttt
     3301 tattactgaa aatgacttct acactaaatg attggtatat tccaaatcct ttacacgagc
     3361 tcttgctgtt ttaatctgat ctaaactaac tttagtatga tagttttcaa tattttcttt
     3421 taattggtcg acgctacttg cacctacaat gatggaacca agcacgtcat gtgatgttaa
     3481 ataactaaat gtcaatgcag ataaattact ttcaatttct ttaatagagg ctattgtttc
     3541 acccaattca tcatgagaat aatcaaaaat accatcttta aatttattgt cgagcacatt
     3601 aacactgttt gaagttaata atcctttaga cacaggtcct cttgccaata ctttaacatc
     3661 attgttgtga attgcatcta ataatgattc tggacgatta tcaatcaaat tgaattgaga
     3721 cattatcgtt tcgatttgac tatgttttaa ataataatca attacatttg ggcgaataga
     3781 agaaataccg taagcacgta taattccttc ttgtttcaat tcatcaaatg cgcttattgt
     3841 ttcgtctaat gggtcatcaa tggttccgcc atgaagttga tataaatcga tatgatcgat
     3901 acctaaacgc tttagtgaac ctttaactgc ctctttaata taggatttac tcggatccca
     3961 ttttgtactg ccatcttttg ttaaacgatt gcctactttt gtaccaatga caatatcatc
     4021 gcgttgttga tatttaagaa gtgccttacc aacaactgat tcatttatac ctttatcgta
     4081 catatctgct gtatcaaaat aagtgatacc attttcaaca gcacaatcta taatttgttc
     4141 ggcttttttt aaatctgtac ctaaactcat gcaacctaga cctaattcag ataatgaaat
     4201 accacttttt aatatatttt tttgcatgtc tgttctcctt tcgatacact ttagctaaca
     4261 agcatagtgt atcaatatat aatatgaatt taaaggaacg tgattataat ggatttaaat
     4321 gaaaaaacaa ttgatcgaac agttatttat aatggcaaaa ttgtagatgt agaaattcat
     4381 acagtgacat taccaaacgg tgaaacatca acaagagaat tagtttatca taatggtgca
     4441 gttgctgttt gtgcagtgac acctaaaaaa gaagtcgtat tagtgaaaca gtatcgtaaa
     4501 ccagtagaaa aaccattgct agaaattcca gctggtaaat tagaagatga tgaagataga
     4561 gtcgaagcgg caaagcgtga attagaagaa gaaacaggat atattgccaa agagttaaca
     4621 catgttgtag atatgtatgg ttcaccaggt ttttgcgatg aacaattatc aatatatttt
     4681 acggataatt tagaagaagg tacagttcac ttagatgaag atgaatttgt cgaagtcatt
     4741 aaagttccga ttgagaatgt taaatcgatg ctaatgaata aagaaataga agatgcgaag
     4801 actatcattg cattgcaaca cctattatta aattataatc attctaaata acaaatgaga
     4861 aaagcttgca ttttattgag aaaactgtta gttttaattg tgaagtttga aataatttgt
     4921 aatgatttta attattagta ggggagtgga catcgttgga agaacgatta aatcgcgtta
     4981 agcaacaatt acaacaatca tcatataagc taacgccaca acgcgaagct actgttagag
     5041 ttctaattga aaatgaaaaa gatcatctaa gtgctgaaga cgtatatctg aaagtaaaag
     5101 ataaagcgcc tgaaattggc ttggcgacag tatacagaac gttagagttg ttagctgaac
     5161 taaaagttgt cgacaaaatt aactttggtg atggcgtcgc tcgttttgat ttaagaaaag
     5221 aaggcgcaaa acatttccac catcatttag tatgtatgga atgtggtcgt gtagatgaaa
     5281 tcgatgaaga tttgttacca gaagttgaaa atcgagttga aaatgagttc aattttaaaa
     5341 ttttagatca tcgtttaact ttccatggtg tgtgtgaaac gtgccaagct aaaggtaaag
     5401 gatagtaaat tgcgtaggtt aaattaacct tcgctttttt tagaggtgtg gttatggaaa
     5461 caattattga agaatatttg cgttttatac aaattgaaaa aggactaagt tctaacacaa
     5521 ttggtgctta tagacgtgat ttgaaaaagt atcaagatta tatgactgaa catcatatct
     5581 cgcatattga ttttatagat cgacaattaa ttcaagagtg tttggggcat ttaatagacc
     5641 aagggcaatc tgctaaatct attgcgcgat ttatttcaac aatccgtagt tttcatcaat
     5701 ttgctataag agaaaaatat gcggcgaaag atccaacggt attattagat tcaccaaaat
     5761 atgacaaaaa attgcctgac gttttaaatg ttgacgaagt gttggcttta ttagaaacgc
     5821 cggatctaaa taaaattaat ggttatcgtg atcgtacgat gttagaactt ctgtacgcaa
     5881 cgggaatgcg tgtatctgaa ttgatacatt tagagttaga aaacgtgaac ttaataatgg
     5941 gatttgtacg cgtatttggt aaaggcgata aagaaagaat tgtaccatta ggcgacgcag
     6001 tcattgagta cttaactact tatattgaaa cgattagacc gcaactttta aaaaagactg
     6061 ttaccgaagt cttattttta aatatgcatg gtaaaccttt atcacgacaa gcaatatgga
     6121 aaatgattaa acaaaatggt gtaaaggcaa acattaaaaa gacgttaacg ccacatacgt
     6181 tacgccactc ttttgcgaca catttattgg aaaatggcgc agatttaaga gcagtgcaag
     6241 agatgttagg tcactctgac atatctacta cgcaactcta tacacatgtt tcaaaatctc
     6301 aaattagaaa aatgtataac caatttcatc ctagagcata aagtgaacca taactcaaaa
     6361 gtcacaatac acatgactaa aaatgtctgt gctattgtgg cttttttaaa ttggttgatt
     6421 aattacgtct atgttttctt aattgaatcg cttcttcttt tgctgaaatc acttctgaac
     6481 gatcacgccg catgtgatgg tctacaataa aaggatctgt tgatgtttcc tgattataat
     6541 catagtctgg atagttggcc ttgatgatgc gttcaaagtc tggagttatt ggtaatataa
     6601 cagatgaaaa aggctttgct gcattcaatt ttgcaatctg ttgctcaatt aacaattgat
     6661 aatcatttaa attaaggtat aacgcatctc tatctttagc attttgtatt atttctttag
     6721 atttattaaa agacttatag gcgcctttta aattattgcg gcgataatgg taacaagcag
     6781 ttgcaaacaa gattaaacta acaactgcat cttgcttact gtagttattt tcagctttcc
     6841 atgcatcttc taaaatgtca tgacatagga aataatgttg cttagtatga aattgataat
     6901 agaaatttat cagtgcctgt tgcattttgt tatcacccca atttaaaagt aagttatttt
     6961 catgctataa tattttagag aattatgcac atatgacgca atacgaggta gatattatgt
     7021 atgaagttaa attagatgct ttcaatggac cattagattt attactgcat cttatccaaa
     7081 aatttgaaat agatatttat gatattccta tgcaagcatt aacagagcag tatatgcagt
     7141 acgttcatgc aatgaaacag cttgaaatta atattgcaag tgaataccta gtattagcgt
     7201 cagaactctt aatgattaaa agtaagatgc tattaccaca atcaacatca gatatggatg
     7261 ttgatgatga cccacgggaa gatttagttg ggcgtttaa
//
LOCUS       DAMZLE010000111         7105 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMZLE010000111 DAMZLE010000000
VERSION     DAMZLE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.112"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            230..1213
                     /locus_tag="POB92_002099"
     CDS             230..1213
                     /locus_tag="POB92_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI6579571.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1404..2330
                     /gene="pstC"
                     /locus_tag="POB92_002100"
     CDS             1404..2330
                     /gene="pstC"
                     /locus_tag="POB92_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDI6579572.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2332..3249
                     /gene="pstA"
                     /locus_tag="POB92_002101"
     CDS             2332..3249
                     /gene="pstA"
                     /locus_tag="POB92_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI6579573.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGILGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3296..4147
                     /locus_tag="POB92_002102"
     CDS             3296..4147
                     /locus_tag="POB92_002102"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDI6579574.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4154..4795
                     /gene="phoU"
                     /locus_tag="POB92_002103"
     CDS             4154..4795
                     /gene="phoU"
                     /locus_tag="POB92_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI6579575.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4934..6748)
                     /gene="pepF"
                     /locus_tag="POB92_002104"
     CDS             complement(4934..6748)
                     /gene="pepF"
                     /locus_tag="POB92_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI6579576.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKEPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            6953..>7105
                     /locus_tag="POB92_002105"
     CDS             6953..>7105
                     /locus_tag="POB92_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579577.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPN"
BASE COUNT         2477 a         1099 c         1201 g         2328 t
ORIGIN      
        1 cctcattttt ttgtgtacat atttaaattt cgataatttt tcaggaagca ttttaatttt
       61 actaatgaag caatattttt tagattaaca aaaattaata tttacatttt cttaacaaat
      121 ttttatgtaa catttacagt ttctaaaaat gaggttaata attcaaggtt aagataaaga
      181 tgtaatcaat acaaatacta tttgttgttc atacagggag gatatttcaa tgaaaaaatg
      241 gcaatttgtt ggtactacag ctttaggtgc aacactatta ttaggtgctt gtggtggcgg
      301 taatggtggc agtggtaata gtgatttaaa aggggaagct aaaggtgatg gctcatcaac
      361 agtagcacca attgtggaga aattaaatga aaaatgggct caagatcact cggatgctaa
      421 aatctcagca ggacaagctg gtacaggtgc tggtttccaa aaattcattg caggagatat
      481 cgacttcgct gatgcttcta gaccaattaa agatgaagag aagcaaaaat tacaagataa
      541 gaatatcaaa tacaaagaat ttaaaattgc gcaagatggt gtaacggttg ctgtaaataa
      601 agaaaatgat tttgtagatg aattagacaa acagcaatta aaagcaattt attctggaaa
      661 agctaaaaca tggaaagatg ttaatagtaa atggccagat aaaaaaataa atgctgtatc
      721 accaaactca agtcatggta cttatgactt ctttgaaaat gaagtaatga ataaagaaga
      781 tattaaagca gaaaaaaatg ctgatacaaa tgctatcgtt tcttctgtaa cgaaaaacaa
      841 agagggaatc ggatactttg gatataactt ctacgtacaa aataaagata aattaaaaga
      901 agttaaaatc aaagatgaaa atggtaaagc aacagagcct acgaaaaaaa caattcaaga
      961 taactcttat gcattaagta gaccattatt catttatgta aatgaaaaag cattgaaaga
     1021 taataaagta atgtcagaat ttatcaaatt cgtcttagaa gataaaggta aagcagctga
     1081 agaagctgga tatgtagcag caccagagaa aacatacaaa tcacaattag atgatttaaa
     1141 agcatttatt gataaaaatc aaaaatcaga cgacaagaaa tctgatgata aaaagtctga
     1201 agacaaaaaa taataagacg caatttcaaa tgtgtcttga aacatgattt tgatggtgaa
     1261 tcattattta gagtacaaag cttgatttat cgagacgctg attttgacat tcagttagtc
     1321 tacaagctta tcaacttaaa atagtggttc atcattattt tacaaatcta attattttgg
     1381 gagtaataga aagaggtttg attatgactt catctactaa tgttaaagct ttaatcgaaa
     1441 aaaataataa taaaaaaggg aagcataatg acaaaattat accagttatt ttagccgcaa
     1501 tttcagcgat ttccatttta acaacactag gtatattaat cacattgctt ttagaaacca
     1561 tcactttttt caccagaatt ccaataactg aatttctatt ttctactact tggaatccta
     1621 ccggttcaga ccctaagttt ggtatctggg cattgataat agggacttta aaaatcacag
     1681 ttattgcgac tatatttgca gttccagtcg ggcttggtgc agcaatttat ttaagtgaat
     1741 atgcaagtga ccgtgcacga cgcataatta aaccgatatt agaaatttta gcaggaatac
     1801 caacaattgt gtttggtttc tttgcattaa cctttgttac accagtatta agatctttca
     1861 taccaggtct tggagagttt aatgctataa gtcccggctt agttgtcggt attatgattg
     1921 tccctctcat cacaagtttg agtgaggatg caatggcatc tgtaccaaat aaaattcgag
     1981 aaggtgccta tggacttgga gcaactaaat tagaagtagc aactaaagtc gtacttcccg
     2041 cagcaacatc aggtattgta gcttcaatcg ttctcgcgat ttcaagagca attggagaaa
     2101 cgatgattgt atcattagcg gcaggtagtt cgccaacagc ttcattaagt ttaacaagtt
     2161 cgattcaaac aatgactgga tatattgttg agatagcgac aggtgatgca acatttggat
     2221 caaatattta ttacagtatt tatgctgtag ggttcacact atttatcttt accttaatca
     2281 tgaatttact ttctcagtgg atttctaagc gttttaggga ggagtattaa tatggaaacg
     2341 acagataata atagacaatc actcgtcgat caacaacttg tccaaaaaca tttatcatcc
     2401 agaacggtta aaaataaagt gttcaaactc atatttttag catgtacatt attaggactt
     2461 gtcgtactta ttgcgttgtt aactcaaaca ttgattaaag gggtaagtca tttaaattta
     2521 cagtttttca ctaatttttc ttcttcaaca ccatctatgg ctggcgttaa aggcgcgtta
     2581 atcggttcac tttggttaat gttaagtatc attccattat caatcatcct aggaataggt
     2641 acagctatat acttagaaga atatgcgaaa aacaacaaat ttactcagtt tgttaaaatc
     2701 agtatttcca atttagctgg tgtaccatca gttgtatttg ggatattagg ttacactttg
     2761 ttcgttggtg gtgcggggat tgaagccttg aaaatgggta acagtatatt ggcagcagcg
     2821 ctaacaatga ccttactgat attaccaatt attattgttt caagtcagga agcaattaga
     2881 gctgtaccta actcagtacg cgaagcttct tatggtttag gtgctaataa atggcaaacg
     2941 ataagacgtg ttgtcttacc agcagcgtta cctggtattt taactggatt cattttgtct
     3001 ctttcaagag cactgggaga aacagcgcca cttgtgctaa tcggtatacc gactatatta
     3061 ttggcaacac ctagaagtat attggatcaa ttttcagcat taccaatcca aatatttact
     3121 tgggcgaaaa tgcctcaaga agaattccag aatgttgcat cggcaggcat tatcgtttta
     3181 ctagttatct taatcttaat gaatggcgtt gcgattattt tacgtaacaa atttagtaaa
     3241 aaattctaat ttaaacaatc aatctcattt atctattaaa aagggagttt taaatatggc
     3301 gcaaacactt gcacaaacta aacaaatatc tcaaagtcat acgtttgatg tctcacaaag
     3361 tcatcataaa acaccagatg atacaaactc acattctgtt atatattcaa cacaaaattt
     3421 agacttatgg tatggcgaaa atcatgcatt acaaaatatt aatttagata tttatgaaaa
     3481 ccaaattact gccattatag gtccatctgg ttgtggcaaa tcaacatata ttaaaacttt
     3541 gaatcgaatg gttgagttag taccttctgt gaaaacagct ggtaaaatat tatatcgaga
     3601 tcaagacatt tttgatcaaa aatattctaa agaacaatta cgtacaaatg tgggcatggt
     3661 ctttcaacaa cctaatccat ttccaaaatc aatatacgat aatattactt acggtccaaa
     3721 gattcacggt attaaaaata aaaaagttct tgatgaaatc gttgagaaat cattacgtgg
     3781 cgctgcaatt tgggatgaat taaaggatag gttgcacaca aatgcatata gtttatccgg
     3841 tgggcaacaa caacgtgttt gtatcgcgcg ttgtttagca attgaacctg aagtcatttt
     3901 aatggatgaa ccgacatcag cattagatcc aatctcaaca ttaagagtag aagagttggt
     3961 tcaagaacta aaagaaaagt atacaattat tatggttaca cataatatgc aacaagcagc
     4021 tcgtgtatca gataaaactg catttttctt aaatggttat gtcaatgaat atgatgatac
     4081 tgataaaatt ttctctaacc catcaaacaa gaaaacagaa gattatattt caggaaggtt
     4141 tggttgatat ataatggcaa taattagaca gcgatatcag gagcaacttg atgatttaat
     4201 aaaggaatta cgtcggttag gtgcaaatgt ctatgtgagt attgaaaatg gtataaaatc
     4261 attaagtatt gacgatagag gctttgcacg acaaacagtt aaaaacgata aacatatcaa
     4321 tcaattaaat tatgatatta atgagcgagt tatcatgtta attacaaagc aacagcccat
     4381 tgcgagtgat ttgcgtatga tgatttcttc attaaaaatc gcctccgatt tagaaagaat
     4441 aggagataat gcatcgagta ttgccaatat tcgattgcgt acaaagatta cagatgatta
     4501 tgtgttaacc cgtttaaaga caatgggtaa attagctatg ttaatgttaa aggacttaga
     4561 tcaagcattt aaaaagaaag ataccgtatt aataagagaa ataattgagc gtgatgaaga
     4621 tatcgatgac ttatatagtc atattattaa cgcaacgtat cttattgata acgatccatt
     4681 tgtcgctgca caagctcatt tagcagcaag acatttagaa cgtattggtg atcatattat
     4741 taacatcgct gaaagtgttt atttttattt aacaggtaca cattacgaac aataacttaa
     4801 agttattact ataaaatccc ttacgataaa tatacatttc tatcattcat aaaccctcaa
     4861 aaaaccaaga ttctcacaat tagtaatgtg aaaatcttgg tttatattgt tctactataa
     4921 attgtctcgc atcttagtta tttgcttgct caatttcatc tgttaatttt tcaacttcat
     4981 cgactaaatc agaaatatat tgaattgtag atttaagtgg ctgttctgta gtaatgtcta
     5041 cacctgcaat gtttgcaagt tcgacaggtg atacactacc accttttttc aatgtttcta
     5101 accaagcatc aacagctggt tggccttcat ttttaatctt ttgagaaacg acagttccga
     5161 ttgttaagcc agcagaatac gtatacgaat ataatcccat atagtaatga ggttgacgca
     5221 tccatgttaa ttcagcaccc tcagtcatgt ctactgcatc tccaaaaaat tgtttataaa
     5281 catttagcat tatttcattt aatgtcggcg catttaaaga ttcaccttga tctactttgt
     5341 gatacacttc acgttgataa gcagcttcta ataaatgggt aaccatatta tgataatatg
     5401 ttctagataa aattgagcca ataacccaac gcttaaatct tggattatca cttgtgttaa
     5461 ataaataatt ggccatcaac atttcattca ttgtagaagg ggcttcaaca aagtacattg
     5521 atgcttctga ttcaagatat ggttgatgtt tttgagctaa tgtaaaatga cctgcatgac
     5581 ctaattcatg tgctaagaca aatgcttcag ccattttacc agtccaagaa ataaacacat
     5641 atgaatgtgt aaagtatgga cttgcacaaa atgcgcctgt atctttacct ttattttgtg
     5701 caaaatcaat ccatcgctga tcgtatgctt cacgtaacat gtttgtatag tcatcaccta
     5761 aaacacttaa cgcaccgaaa atataatttt ttgagtcttc aattgaaatc tctggttcat
     5821 aatcagggtc tacagaaatc ttcaagtctt caaaacgcat gttatctaat ccgtgaatac
     5881 gttgtaaaag tttagcatat ttctgcataa ctggtgccaa gtcacgcata atcatatcga
     5941 tttgacggtc aaacatatca cgcgttactt cttgactatg taataaataa tcgatgactg
     6001 attcaaatcc acgtaaatca gcttcaattt tttcttgttg tacttgcata ttatatgtag
     6061 ccgcggtagt atgctgatat tttcgaatcc catcgctaaa cgatttgaaa cttttacgtc
     6121 taaactcagg ctctttatta tcttcataat cattttcaaa cgtagcatag tcgacagggt
     6181 acgttgtacc atcatgttca aatgaatcga atgtaatatc tagcattttt gtcgtgccgt
     6241 ataaatcgta aggactgttt agcgtaggtg ataaagttgc taatacttgt tctaccgacg
     6301 cagataattg gaatggcttt tgttttatta actgtttaat atagtgttga tatggacatg
     6361 attcttctaa ttgttgaagt atttcttctg gtaattcaag tatttctgac tctacaaatg
     6421 ataattggct aacaatttta ccgtatgtag tagataattt agcgctcaat acttgtgcct
     6481 cgatattact agtatctaca cttaaacgta gttctgcata attacttaag cgatctaagg
     6541 caattaaaat attttctaat tcagctaaag cagtattaat ttgttcgatt gaatttaatg
     6601 ttgttgcata tgtatgatga aattgatttg cttgttgtac tagagcgtca atactttcat
     6661 aatattgttg atcatcttta aataagtcta ctaaatccca tgtttctgaa acaggaacat
     6721 cttctcttaa aggtaaacct tgagacatat aatcactcct ttggtataat acattcatta
     6781 tacataattt tgtctcctaa acgataatgt tacgatttcc atgtcgcaag gaacaatgat
     6841 ttttctaata gtagatagaa tattgcttga aactgactgc tattgtttaa gtttttaaaa
     6901 atttataata aaatgtatac aaggaaagta atgaaataaa aggagaatga atatgacatt
     6961 attcgatatg cctaattatt tatggatcac aacattaata atgattttat taacaatatt
     7021 ctgttgctta gttttaaata aatggtttgt atctgcagta attacatttg ttatattagg
     7081 tgtgcttgca ttttttattc caaat
//
LOCUS       DAMZLE010000112         7070 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMZLE010000112 DAMZLE010000000
VERSION     DAMZLE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.113"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            16..435
                     /locus_tag="POB92_002106"
     CDS             16..435
                     /locus_tag="POB92_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6579578.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            462..572
                     /locus_tag="POB92_002107"
     CDS             462..572
                     /locus_tag="POB92_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579579.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(642..4394)
                     /locus_tag="POB92_002108"
     CDS             complement(642..4394)
                     /locus_tag="POB92_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI6579580.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKAAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTGKLTVAANNGVAQIKPT
                     NSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVY
                     NTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYL
                     YGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLF
                     TTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKS
                     PVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVN
                     GKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRAS
                     VYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKE
                     VKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTIN
                     NRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNE
                     QPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGL
                     QYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFL
                     KGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSN
                     TKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKM
                     RWNPAHPGTHQYATDIDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4622..5056)
                     /locus_tag="POB92_002109"
     CDS             complement(4622..5056)
                     /locus_tag="POB92_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579581.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5211..5681)
                     /locus_tag="POB92_002110"
     CDS             complement(5211..5681)
                     /locus_tag="POB92_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDI6579582.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5729..6946)
                     /locus_tag="POB92_002111"
     CDS             complement(5729..6946)
                     /locus_tag="POB92_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDI6579583.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKTSNENNDSTNNSDSSNQQQPATDQNLNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         2075 a         1156 c         1182 g         2657 t
ORIGIN      
        1 gtggagtaga acaacatgta taaacaactt gaaaaactta ttacactgac taacaatgac
       61 ttaaacttag tgaatagaag atttggacaa cgcacggata tcacatctga acagctagaa
      121 cttctccgta ttttatttaa ttacgatcgc ttatcacagt atgatttgac gatgaagatt
      181 agcagggaac aatctatagt ttcaaggtgg attaagaaat tagttttgaa agggtacatc
      241 acaagtcaac aatctagcga agatttaaga tgtaaagaat taattttgac tgatcaagca
      301 cgtacattaa tttcacaaat aaataatgca cgttgcgaat tgattgaagc aagatgtcaa
      361 tgtttatcgg aagtcgaatt agacaattta aatcaattac ttgataagtt aaatcaacga
      421 cgcatatcgt tgtaacacga caatagcaaa cacataattt agtgtcgttg tgcacacaac
      481 aaaaattgaa tcatgaatta caagcaaaag tagcggtgat tgttaaaatt gatggtaaac
      541 aatcaccgct atttttgctt gtgtatgtat aaaaaaggga tcaaaggtca tcccccatga
      601 ttgatagtgg gggatgactt ttgatcctat gttcatgttg cttatttata ttgtgggatg
      661 tcgaagtatt tgccgacttc gccaatttta tcatagtagc ctttgatgat tttagcattg
      721 atgttagccc aatctatatc tgtagcatat tggtgtgttc ctggatgtgc aggattccat
      781 ctcattttgt aaagtgtatt ttgaccagct tttacatatg agttgccgat gaatttagca
      841 ccaccaacga ttgcttttga tactgtgtcc caaccagctt gtttagcata tttaatacct
      901 tcacgtaaag gatcgttatc atatgcagca ataccaaata cgttatggta tttcgtgttt
      961 gagttagtta caactttgtt gttcactaca tctgcacctt tcgctaattg agaagtaccg
     1021 ttacctgttt ctaatagggc atgtgagata agataaactt cattaatgcc atacatttga
     1081 gcagctttgt taaatgcagc accttggttt tctaatacac ctttaccttt taagaattga
     1141 ttgattttat caatagaaat attttgtggt tggtctaagc gtaagaattg atattttaat
     1201 gctggatctt gagctaaacg cttcgtatcc attgcatgct taacatcatt aaagttagca
     1261 tctgtccact tacctggtac acgttgtact tgtggtttat attgtaaacc agcttgtatt
     1321 tgagcaactt ggtttaatgt catacctgtt tgattatact taattaattc tttagctaaa
     1381 tcagttgatt taatccatgc taatttaccg ttagataatt taccatagta ccaagtttgt
     1441 ccattaatga cttgttcttt aacaactgcg aatggttgtt cattaaatgc ttttaatgag
     1501 tatttagctg tatcagaatt tggtgttaca tagtaataac cattaccatt tttaattaca
     1561 taagtgtagt tataatcttt ggcagctgat gtagttggtt taacagcagt tggtgcagtt
     1621 aaatcttttg catttaccca accagtgcgg ttattaatag taccgtataa ataaacatct
     1681 ttgcctacag atacttgttt cgttgcatta aatgtacctt gagcaatgtt attgcctgtt
     1741 aaaatgactt ggtttttagt accccaagga accattgata agccgttatt tgatttatta
     1801 acagtatatt tttgagtcgt tttaacttct ttgcctaagt tttgaacatt taagtctttt
     1861 acattgaacc aacctaatgg gatgttatgg cttgtattgt ttaataatac atacgtttca
     1921 ttaccatgag cacgctcttt tgttacatag aacgtacggt ctgcatattt cgcaccgttt
     1981 ttcgctgttt tttcataaac agaagcacga ataccagtgt tgtttggttt aacttgagca
     2041 atcttgctaa ctgtttgagt cgtttgtggt ttagtaacag tataagcttt tacagctgtt
     2101 tttggttgtg ctactgcttt tttaggtgca gcaggtacag ctaaatatgc tttacttacc
     2161 caaccagatt taccatttac agttccaaat aaatagatag atttatcaat ttgttgttgc
     2221 ttagtcgctt taaaagtttg gttacctgta ccagaaactg caccagcttc ttgtttataa
     2281 gtgccccaag gtactgaata taatttagtg cctggtttta ctgtatatgt ttgcattaca
     2341 tttacaggtg attttgcatt gttataaata acgtcacctt gtttaaccca accaattaaa
     2401 gttggactat tgtaatcttt aactaagtag aatttgtttc cacctaaact tgcttctttt
     2461 gttacagcaa atgttttttg aacttctttc gttggcttac cagttttgtc ataaactgta
     2521 gtgaataagc cattgttttt agcattaatt tgagcaacac cgtttaatga tgaaactgtt
     2581 aatttattat ttgttgtagg tgttgatggc ttaggtgttg gtgtaggcgt aggtttagca
     2641 gtatcaacta aatatgcttt acttacccaa ccagatttac cattcacaga gccatataaa
     2701 taaattgatt tatcaatttg ttgttgcttt gaagccttaa atgtttggtt tccagagcca
     2761 gacacactac cagcaacttg tttagatgta ccccaaggta ctgtataaag tttcgtacca
     2821 ggtttgattg aatatgattg atttacattt acaggtgatt tagctgtgtt gtaaaccaca
     2881 tcgccttctt taacccaacc aaatttatta ccagaattgt aatcttgaac aagatagaat
     2941 ttttgattac ctaatgtagc tgttttagat acagcaaatg ttttttgaac ttcattagtt
     3001 gctttaccag ttttgtcgta tacagtagta tataaaccac tatttgttgg tttgatttgt
     3061 gcgacaccat tgtttgcagc aactgttaat ttaccagttg atggtttcga cggtgtagta
     3121 ggggtagttg tagattgcgt accccatggc gccactttac ccatttttat taaatatttt
     3181 tcattaatta agtcatataa ttgatcataa ctataattat gacttcttaa atatccatgt
     3241 ggatcggcat ggtcagtacc acctaaatat ttacttacag cgtagtgagt ccatactgta
     3301 ccatttccat catactcagc actgtctggt tttaaaccat aatattgtaa ttgtgtagct
     3361 gcatagtcag catagttatt cattgaacgt gcaaatgaag catagtcgtg tgtgtgtacg
     3421 atttcaacat tgatgaatct agggttaccg actgcaccga caccccaaga taagtaatcc
     3481 gttggtgctg tttcgattat acgatcccca tcaacaaatg catgtacgaa tgcgttttga
     3541 tagttatttt tcatataact aatttcacca tttatcgtcg aacgatcatt agctgtatca
     3601 tgaactacga taccttcagg acgacctacg ccgttacggt atgcgtattt agggaagtaa
     3661 gatgtataat cttcttcaat tttaggtgct tttaagttat ttttacgaat gtaatcgtta
     3721 attgaagagt ttacttgtgg tttatatttt ggcaaactcg tttttggtgt tgcagcaact
     3781 gatcttggtt gtgctgaagc gctaaaagta gttactttag gtgtcgcttc agttttagct
     3841 ttaggtgctg atgtagttgc agctttaggt gctgcggctt tatattgcgt ttcaagagct
     3901 gcaggtttag cagctgattt aattaattct ggattaattt gattttctga attatcatct
     3961 tcatcatcaa ctaaactata accagcattt gtaacattag tgttagtttt aggtgctgta
     4021 gtgcttgttg actttgcaac aggctgcgta ttatttgtag tcgctgattg attagcacga
     4081 gtgtcaccat ttacttgtgc agtatcaact ttttgactta cttgagcatt gcctgttttg
     4141 ttatttgctg tttttggttg gacaatagca gggtcttgat atacttgagt gccagaaatg
     4201 ttttgcgttg gattttttac ctcagctttt gcttgttcag tagttgcttt aactttatta
     4261 ctatctaaaa cgtttttatt agtagtttga tcttgtgtcg tctcagctgc ttgaacttga
     4321 tgtgcagtga ctgctgaacc tacaagcgtt aatgcaacca ttgatggtag tttgtaattg
     4381 aattttttcg ccattctatt tattactcct aacatttatt aattattact aacattatag
     4441 tacctgtttt atatacctgt gcgtatttaa ccaatttgta atatactctt aatttacatt
     4501 ttcactgtaa aaaaatgaaa aaaattttga atctcaatgg tgacgtgaca aatcaaatag
     4561 aattgatgtc aatttaagaa ttggtttatt acaaatgttt ctaaaataga tacaactttt
     4621 tttaattaag cgaggttaac ttttttgtca tttcaatatg ctcgatgcct tcctcaagaa
     4681 atatattacc tctcatttta aagtttaaac tttcataaaa tgggatagca tgacattggg
     4741 cattcatagt agctacgtaa aaaccttcat ctttagctaa tgattctaca gcttgcataa
     4801 gcattctacc cattccttgt ccacgatgtg atttcatcac agctactcgt tctattttga
     4861 cagttgtttc attaataggg cgtattcgag cagtggcaac tggctgtcca ttatcatatc
     4921 caatgaggtg aatagattca gattcatatt catcaatttc actttcctca gggacgcctt
     4981 gttcttctac aaacactttc tttcttatat agaagcaatc ttctaacatc ttttgattgt
     5041 ttacttttga aaacataaaa ttcctccaca tcctaacaaa atcatttaca tttaatttac
     5101 taataataac atagaataac gattgttatt ttcctcaaaa ataattagac ataaaaaagc
     5161 tataaagatg attgttgtga atcatcttta tagctaatta attattaatg ttatagtgct
     5221 tcttttctaa taattgctgt atattgccaa aagattcgca tttgagcaat attccaatta
     5281 ttcattccca ttgtaaatcc tgaaggctta aatatattca catcagttac ttctaaagct
     5341 gctgcaacta aatattgtaa tggaatgcta agtgatttca ttgtttcggt aatgccattt
     5401 tcatcatata cccatgaaaa tggtaactct gattcgaaaa catcaacact attgaaaatt
     5461 tcaggtaggg ctaaaatata acatgcacca tctacaatag gattgtcgat actatttttg
     5521 taataaatta ggagtgcttc atagttttct cgatgctcat gattgacgta aaaaaattca
     5581 cttctaaaat gagccatatc ttggctatga atgatttgtt gttctaacat attaaacatg
     5641 ccatttatat ttgcaatctt ttcgtatgtt tttctactca tgtatgatgc tcctttcata
     5701 aatagtaaat gttaatcgtc atataatatt aatttacaac accattttgg ttatttgaag
     5761 cttgtggcgc ttgttgtgtg ccaccttgat tttgatttaa gttttgatct gtagcaggtt
     5821 gttgttgatt gctggaatca ctgttattag ttgaatcatt gttctcgtta gatgtcttat
     5881 ctttatctgt cgtatcattt tgttcttttc tcaataaact actatctaaa ggcgttaatg
     5941 gtattaatga accataatga ttaatgacac gttgatctaa gaaatcattt ttatcattaa
     6001 taggtgataa ttctaagtct ttacgaagta agtttgcata tttttgaatg ctttcaacac
     6061 ttggatgata atagtaaata ccatttaaca tatcatcttt accttttaat tgcgcagttt
     6121 taatttcaac atcatttgtt aagtacattt ttgctaaagc tttaatttca gagttagtta
     6181 aattatgctt tgcattttta cctacaattt gaatcacgtt atcaagttta tcaatagaat
     6241 caacttcctg tgctttttgg aataaaatct taattaattc catttgacgt tgtccacgtt
     6301 ttaagtctga atcatgatgt ctagttctag caactgctaa agcctcatca ccatttaatt
     6361 tttggtaccc ttttttaatt ttaatcttac cagtatcatc tgtgttaggt tcatttaagt
     6421 cgtatggcac atcatagtat atgccaccaa gctcatttac agcctcgaca aatgctttca
     6481 tattgactct cacataataa tcaacaggta cattcatggt agcttctacc gaatccattg
     6541 cggcaattgg accaccatat gcatgtgcat gggtaatctt atcgtaatag ccaactttag
     6601 gaatgtagct gatagtatca cgtggaatac taagcattct aatttgatgt tttgattgat
     6661 taaaagtagt taaaatcata gcgtctgatc tagagtgttc agcatcctgt cctttttttc
     6721 ttcttccatc gttatcatcg atacctaaga aaagaataga gataggttgt tcttcgggat
     6781 tgactttatt atctcttaag ttggattgac gattagcatt tttgctgtct tgagaagatt
     6841 cgaatgcatc ttgggacgtt ttaaaaagta acgtagcgaa gactattgga acaacaatga
     6901 gaaccaatgc tagaaggatc aaaaagtatt ttaaaaattt attcatgatt gatgctccta
     6961 tattaaattt ttgttaaatc ataatcataa ttttaaaata taattagttg aacgatattt
     7021 atacaacttc attactaata cattatagcc ttaaattgta gtactttata
//
LOCUS       DAMZLE010000113         6994 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMZLE010000113 DAMZLE010000000
VERSION     DAMZLE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.114"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            35..841
                     /locus_tag="POB92_002112"
     CDS             35..841
                     /locus_tag="POB92_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6579584.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(904..1560)
                     /locus_tag="POB92_002113"
     CDS             complement(904..1560)
                     /locus_tag="POB92_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDI6579585.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1673..2146
                     /locus_tag="POB92_002114"
     CDS             1673..2146
                     /locus_tag="POB92_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6579586.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCISELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2269..3480)
                     /locus_tag="POB92_002115"
     CDS             complement(2269..3480)
                     /locus_tag="POB92_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6579587.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(3662..4321)
                     /locus_tag="POB92_002116"
     CDS             complement(3662..4321)
                     /locus_tag="POB92_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579588.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDENGGA
                     N"
     gene            complement(4381..5523)
                     /locus_tag="POB92_002117"
     CDS             complement(4381..5523)
                     /locus_tag="POB92_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6579589.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVQFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            5781..6167
                     /gene="gtxA"
                     /locus_tag="POB92_002118"
     CDS             5781..6167
                     /gene="gtxA"
                     /locus_tag="POB92_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDI6579590.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            6301..6408
                     /locus_tag="POB92_002119"
     CDS             6301..6408
                     /locus_tag="POB92_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6579591.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         2303 a         1092 c         1303 g         2296 t
ORIGIN      
        1 ataacatttt aatttgagta aagaaggtat gggtatgaag atagggacaa tcgccgattt
       61 acatattgat cgtcataata aaaaaacatc cgaagattac ttagaggcat tggttgaaat
      121 agttaagtat aagaaattgg atatattgtt gattgcggga gatatctcaa atcattatca
      181 attgacgcac caatttataa cgcaattgac aaaacaatta gatatacctg tgaaatttgt
      241 tcctggaaac catgatttat gggaagttga aagtatgact acgcaagaca tttggaataa
      301 ttataagagc atgtcacagt gcttggtagg aaaaccattt atagtaaatg aagaatgggc
      361 aataatagga catacaggtt ggtatgatta tagctttgca gcacaacgat tttcattaga
      421 acatttacaa aaagggaaac attatggtgc gacttggcaa gataaagaac gaatatcttg
      481 gggcatatca gatcaaaatt tatctaaaat agcggctgaa caagtgaaga aagatatatt
      541 agaagtagga aatagacgag tgattttagt cacacatgtt gtgacgcacc ctgatttcat
      601 tgttcctatg ccgcatcgta tattcgattt ttataatgca tttattggga caagtgattt
      661 caatcctttg tatgcgatgt tcgatatacc atatagtatt atgggccatg ttcattttcg
      721 taaaagtgtg atagatgatg gcagatgtta tctctgtccg tgtctaggct atccaagaca
      781 atggcgttca gaagatattt accaggaaat aaatgagacg atacaaataa tagaaattta
      841 aaatgcgcaa acctgaccca gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt
      901 tacttacgtg tgtttatttt gttgttgatt ttcaattgta tatgaatgtg gttgcacata
      961 aatgcacttt cttcctggtg aattaaagct gtattccatt ttctctttac gaattttaat
     1021 aatttgtttg cgatttggaa tgatggcagg taaaactagg ccacgacgaa tatgactcca
     1081 aagtggatcg atataatagt aatagtaatc atcgtaccca atgagtaacg ttacgtgaat
     1141 atttgaaact tgtttcgctg gcttgttatc aaagcggaaa acacgtagta atggtttaga
     1201 gccaagatta gtatggtata ttaacacagg ttgaccttga tcgataatac ctttaagatc
     1261 ttctaaggat ttaccagtgc cgtctacgat attaggattg tatttttgta aaaatggtac
     1321 atatgcttct ggaaatatcg tttgatgata attgccaagc ttaatgaata agtgatgtcc
     1381 aacataacct ttatgtggat tgttcggatg tgtcggccaa tgtctcataa tttctgtagc
     1441 agggatatgt tggttattgt attgcaacat catggctgcg gaaacacctt cacaccccat
     1501 gaccataggg ataggaaata gctgactgat aggtttaact ggtaatattt ttcggttcat
     1561 aatatagtcc tcgcattgat tcaataaata tttaatataa ttatatagcg tcaatgcaaa
     1621 atgtcctaaa catatgtttt acatgagtga ataaaattaa tggagtgata aaatggaata
     1681 tcaattacaa caacttgcgt cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg
     1741 acgacaggct cagcaacata tagcagggtt ttggcaaaga tgttatcaag agggagtaat
     1801 tgcggattta cagttaaaaa ataatggtga tttagccggg atacttggct tatgtatatc
     1861 tgaattagac ggtaagatgt catatatgat tgcagttacc ggagataata gtgctgatat
     1921 tgaaaaatat gatgtcataa cattagcaag ttcaaagtat atggtatttg aagcacaggg
     1981 cgcagtacct aaagcagttc aacaaaaaat ggaagaggtt catcactaca tacatcaata
     2041 tcaagcagat acggtaaaat cagcaccatt ttttgagttg tatcaggatg gtgatactac
     2101 aagtgaaaaa tatattacag aaatttggat gcctgttaaa gggtgattga atagaaatgt
     2161 tcttgatatg aaggtgtaat tttagttgaa gattcgatct cataattcat aaagaagaac
     2221 cctgacaaag atagatatct ttgctagggt tctaattatg ttgaagtgtt atgcgacatg
     2281 ttgcttttta atcattttaa agtaaatgat ttgtagactg actaatagaa tggctgtaat
     2341 gaagataata atcatatatg gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc
     2401 aacagcgcca cctaagataa attggaataa gccaagtaaa cttgatgcgt taccactgcc
     2461 acctgttcgt tcttccatag ccattgtgaa accaagtggt ccaattgacg tcacaggaca
     2521 cacatttaag aagaatgcga tgagtaagac ccaaagtggt aaatggaatg taagtgtcag
     2581 gataattaaa gcaacaccta ccacttgtat aatagttaaa atgattaata atatgtggcg
     2641 atgtaatttt tctactaata aagcaacgac ttgactgaca atgattaaac ctacaccgtt
     2701 aacagcaaac atgatactaa attgttgggg tgtcatatta tacaattttt gagtaataaa
     2761 tggcgatgca gatgaataac taaatagcat tacataagtt aaaccttgta atagcattgg
     2821 aataataaat gctggttttt tcaaaagact tccaaaatct ttaatgacgc tactaaaatt
     2881 cacctgcttt aattcatctt tagatgtttt aggtaattga gaagcgacgc caattaaaat
     2941 gatgagtgcc acaatagtta aaattgtgaa aatagaacgc catgttgcta cggataaagc
     3001 taatccacca gctaatggtg caagaatagt gatgatgcca ttaactacca ttaaagatgc
     3061 taaaaattta gcgagtgcgt tgccactaaa tttatcacca gcagaggctt tggcaatcac
     3121 gatgacgcca ccaccagtta aaccttgaat aaatcgtaaa gttaagaata atggcaattg
     3181 atcaacaaac atagacaaac ctgataccaa aatgaaaatg atcaaaatgg atacagcaat
     3241 ccgtttgcga ccaaaagcat cggataaagg tccaaagata aattggccta gtgcaagacc
     3301 aatcattgtg aatgataatg ttaattgaat ttctgatgta gtagaaccaa aatcaccttg
     3361 cactttaggt aatgatggta cgtacatatc tatagttagt gggccaaaag cggtcatgac
     3421 gcctaacgta attagcaata aaaatgaaaa atgcttcgtt ttggttttcg ttgattgcat
     3481 gatcagactc ctttaaatga tataattata taaattatat acgttttgtg tgtatttgta
     3541 tgtagttaga atacaagttt atataaaaaa tccctatact cttaatgcat catcacgaat
     3601 aacgtagtga tacagataaa gtatagggaa ttaatgttta tcatcaaagc aggtgtaata
     3661 attaattagc gccaccattt tcatctttaa ataagctttc taatttagat ttaatactgt
     3721 cgaattttgt attaatatca tttaacatgc tttcaatttt accttggctt gcattgtttt
     3781 taagtgcatt atttgcctct gtcaatttgt tgatttggtc ttgataaaat tgagttagct
     3841 tagaatcagc tttgttttgt tcttgctgct tcaagttgtc gatttgattt tgtaaatttt
     3901 taataatgtc atcagttttt tggttgtttt taatattttc tttagcatta tcgacttgct
     3961 ggttaatttg gccgtcttga ttttcaactt gttttttata attttgagag actttagggt
     4021 cattactaac tttatcgcta tcattgttaa gtaaggcgcg tgtgacaaaa atagcgacaa
     4081 taattaagag aatagcaata atgatatata accatttttt tgaacggcgg gatttctcgt
     4141 tttcttcttc aagaatttgt tctcttcgat aatctctacc gttatagtat cgttccgggt
     4201 gttcttcagg atcgccttta gcatattgtt gtctatacga tgcgtcttca gattgacgac
     4261 gatgattttg attttgattg tcgtattgat atgaatcacg gtatttatca gttcttttca
     4321 tgggtttcac ctcattcaag ttttaactgc aaataataaa gctgatgatt tactttgaca
     4381 ttattgcaat ttcagaatac gagcaatgtc agttgcagtg tttaataaat tttgttcgct
     4441 attttgtagg acatcttcta aagtgctagg tgaagagatt atagaatagg cactatcaat
     4501 accgaaatcg taaacatgtt gataattttc gcctagactg ccacaaatcg cgatgacagg
     4561 aatatgatat tgttttgcag ctaacgcaac gcctacgggt gttttaccaa agatggtctg
     4621 ataatccatg cgtccttctc cagtaataac gaggtctgca tctttaattc tttgatgaaa
     4681 atctgtaatg tcaaagacga catcaatacc ttttgttaaa gttgtctcac aaaacgctaa
     4741 taatgctgcg cccataccgc cagctgcacc agaacctggt atttgattaa cggactttcc
     4801 tgtgcacatt tttatcttat catgataatg cgacattgcg aaatccaact ttggtatcat
     4861 ctttgcatca gcgccttttt gaggaccata aatataggta gcaccatttt cacccaataa
     4921 aggatttgaa acatcacagg ccactttaaa ggtcacctct tttaatcgcg aatctagatt
     4981 ggttatatcg atttgtgcaa tgtgagcaag attagcacca ttcatttgta ataagtcccc
     5041 gtttacatca gtaaactgaa cgcccagtgc acttagcata cctgtaccac catcatttgt
     5101 tgcactgcca ccaatcccta aaataatggt cttagcacca tgatttaatg catctttaat
     5161 tagttcaccg gtaccatatg atgatgtgta taaaggatta cgttcctctt tttctaataa
     5221 atccaaacct gacgctgccg ccatttcaat aattgcaatt tgttgttcgt ctgcgcgtgc
     5281 ataacatgct tcaataggtc gcattaaagg gtcattaacg atgactgtat acttagtggc
     5341 acctgttgca tgcattaaag catctgtggt accttcacca ccatcagcca tcggaatgat
     5401 atcataatga agggtattcc cataaacatt agtaaatgcc tgttttataa tattgccaac
     5461 ttgctgtgcg gtcatacttt ccttaaatga gtcaggagcg atgacaattt tttgtaattg
     5521 catagtagac atcctctcat tactgttgtc gctcttattg tatataaaat tactagtgaa
     5581 ctaaagtaat ctctatagtg ctaatgagtc acgcgataaa tcagttacat tataatggcg
     5641 tataaggatg agaggaaaga gactaaattt gctgtgaaag ggtataaaga ttgaaataaa
     5701 catatttgaa gtgaagcgga ttggcacgca atttcgagaa cgtagttatg catatatttt
     5761 gatgttgagg agttacacat atgaaactaa cacaaacgca tgctgaaatt ttaaaattta
     5821 tcattgttgg cggcattaat acgttaaatt attatgttgt ctatttattg ctgttaaaat
     5881 tattacacat tgaatatatg attagtcata ttaccggatt tctagtcgct tttgtgattt
     5941 cgtattattt gaattgttat tttgtttaca gggtaaaacc tacttggaga aaattcatta
     6001 gttttccaat tacgcagatt gtcaacgtaa gcttacaaac agttttatta tatgtttttg
     6061 tatcctggct aaatttgcca gcggaaattg cgccatttgc aggtttagtg attacgatac
     6121 ccatcacatt tgtattatct aaatggattt taaaagatag taattaaagt agcgtaaacc
     6181 actaaaaaac gctttactcg aactttaata ttgcgtgaaa tataataaat aaatgatata
     6241 atataatggt cgcctaacat ctcgggcgtt actttaacgc agagaggagg tgtatgacgg
     6301 atgttcaatt tattaattga catcatgact tcagctttaa gcggctgtct tgttgcgttt
     6361 tttgcacatt ggttacgaac gcgcaacaat aaaaaaggtg acaaataacc aacacctaaa
     6421 taaatcccct cactatggca gtagtgaggg gatttggtgt atttagatgt tcaatttatt
     6481 atgtaattaa ttatagcatt gatgaacaag gtgtgcaatg tatttttgta atattacaat
     6541 aaatgaattt aattatatta gaattaagta tttcgaaaaa tgatttaata gttgagtcat
     6601 aggatcatta gataaagcga ataatcaatt atagggacat tgggatacaa atcaatgtac
     6661 tagactctac aaggctatac tcagggaccc aacatagaga aattggatcc acagtttcta
     6721 cagacaatgc aagttggcgg agtcccaact aagagaaatt ggattcccaa tttctagaga
     6781 caatgcaagt tggggtggga cgacgaaata aattttgtga aaatatcatt tctgtcccac
     6841 tcccagaacc actacataat aaatcattag tggctcttta tcatttctgc cccataacct
     6901 tgtgaatttt taaaagaaat gtgtcacaaa tccacgcgac attgataatc gttatcaact
     6961 aatatatgat gtacttgtaa taagtattaa tttt
//
LOCUS       DAMZLE010000114         6978 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMZLE010000114 DAMZLE010000000
VERSION     DAMZLE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.115"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            35..463
                     /locus_tag="POB92_002120"
     CDS             35..463
                     /locus_tag="POB92_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579592.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(672..1178)
                     /locus_tag="POB92_002121"
     CDS             complement(672..1178)
                     /locus_tag="POB92_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579593.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            1291..2214
                     /gene="graX"
                     /locus_tag="POB92_002122"
                     /gene_synonym="apsX"
     CDS             1291..2214
                     /gene="graX"
                     /locus_tag="POB92_002122"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDI6579594.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            2230..2904
                     /gene="graR"
                     /locus_tag="POB92_002123"
                     /gene_synonym="apsR"
     CDS             2230..2904
                     /gene="graR"
                     /locus_tag="POB92_002123"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDI6579595.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            2897..3937
                     /gene="graS"
                     /locus_tag="POB92_002124"
                     /gene_synonym="apsS"
     CDS             2897..3937
                     /gene="graS"
                     /locus_tag="POB92_002124"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDI6579596.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            4081..4842
                     /gene="vraF"
                     /locus_tag="POB92_002125"
     CDS             4081..4842
                     /gene="vraF"
                     /locus_tag="POB92_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDI6579597.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            4832..6721
                     /gene="vraG"
                     /locus_tag="POB92_002126"
     CDS             4832..6721
                     /gene="vraG"
                     /locus_tag="POB92_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDI6579598.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGILMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAVIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKVKNKVDKSIKTRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT         2576 a          872 c         1228 g         2302 t
ORIGIN      
        1 cacgaagtga tttgaaataa ggttgtgaaa gggaatgaca aggtcagcat taaaaccatt
       61 taaaaataaa cgcgttatgg ttactggacg tatacaacgt gttttgttta aaaattattt
      121 agatagacat agcacattta agccgaatgt aaggatatta ttaaaagatg tatttgtttc
      181 aggtgtatca atagatcatt tatggttata tgagacaaat aaatactatg cattggcaat
      241 ggaacttatt catcaacgag taaaatttag tgcgaatgtt gtaccatatt acaaaataaa
      301 tagaaataat aatttattcg tacaagatta tggaattaag cgtaaaggta ggttaattac
      361 tgaagaagct tacaatcaaa acaatcagta tcaggataag atatatgaaa aattaccgga
      421 tatagatttt agactcgaag atttttatag taaggaaaac taaacaacat atataaagca
      481 ccactaatcg tagttgatag ttgatcaatt acacattagt ggtgctttat tttgaattta
      541 aatttgataa atgaataaga gtatacgcat tcatttaaga ttagatgaca ttgataagta
      601 tgagtaatgt attaggtaac cattaaattc gttgttatgc gattctgata caagattatg
      661 ataaaatagc tttaattaag aattttagcc atcatatagt catcgtaata tttaccatcg
      721 ataaataact tatcttttaa aacgccttcg atttgaaaat cggcactttt aaaaagctcg
      781 agggcaggtt ggttattgag tggtacattt gcttcaattc ggtgtatttg attgtttaaa
      841 caccaagcca taatggcatc aagaagtgct tggccaattc cacgatgttg atataatttc
      901 tttacaccta aatcaatttt agcaacatgt ttaatgcgtt gaaatggtgt cgtattaaca
      961 aaggcaaagc caacgagttg ttcatcactt tcagcaacaa agatgacttt atgtggagaa
     1021 gtgatatatt cttctaattg tttactagcc gacgtgacgc taggatcata ttctcctggt
     1081 gtgtagaaca tatacggaga ttcgtcgtat attttcgcta acattgaaat gaaattttct
     1141 acatctttga tactaactct acgtataata tgggccataa aaagcctcca gtattttgaa
     1201 attttaaaac atttgctact attataatat atatgtaact aaaaggtgga gtaatatgtt
     1261 ttttggttac agtttaaagg agtattttag atgaaaccta aagttttatt agcaggtgga
     1321 acaggatata ttggtaagta tttaagtgaa gtgattgaaa atgatgctga actttttact
     1381 atatcaaaat atccagacaa taaaaaaaca gatgatgttg aaatgacttg gattcagtgt
     1441 gatatatttc attacgaaca ggttgttgca gcaatgaatc aaatagatat tgctgtattc
     1501 tttatcgacc caacaaagaa ttctgccaaa ataacacaat catcagcaag agatttaaca
     1561 ttaatcgcag cagataattt tggtcgagca gcggctatca atcaagtaaa aaaagtaatc
     1621 tacatacctg ggagtcgtta tgataatgaa acaattgaac gcctaggtgc atatggcaca
     1681 cctgtagaaa caacaaattt agtttttaaa cgttctttag ttaatgtaga attacaagtt
     1741 tcaaagtatg atgatgttag atcaacgatg aaggtagttt taccaaaggg atggacatta
     1801 aagaacgttg taaaccattt tattgcatgg atgggttaca ctaaaggaac ttttgtgaaa
     1861 acagaaaaat cacatgatca atttaagata tatattaaga ataaggtgcg accgctcgca
     1921 gtatttaaaa tagaagaaac agctgacgga ataataactt taattttatt gagtggaagt
     1981 ttagtgaaaa aatacacagt taatcaaggg aagttagaat ttagattaat caaagagtcg
     2041 gcagtcgttt atatacatct atacgattat atccctcgat tattttggcc gatttattac
     2101 tttatacaag caccaatgca aaaaatgatg attcatggct ttgaagttga ctgccggatt
     2161 aaagattttc aaagtcgatt aaaatcagga gaaaatatga aatatactaa atgatattgg
     2221 gtgatatgga tgcaaatact actagtagaa gatgacaata ctttgtttca agaattgaaa
     2281 aaagaattag aacaatggga ttttaatgtt gctggtattg aagatttcgg caaagtaatg
     2341 gatacatttg aaagttttaa tcctgaaatt gttatattgg atgttcaatt acctaaatat
     2401 gatgggtttt attggtgcag aaaaatgaga gaagtttcca acgtaccaat attattttta
     2461 tcatctcgtg ataatccaat ggatcaagtg atgagtatgg aacttggcgc agatgattat
     2521 atgcaaaaac ctttctatac caatgtatta attgctaaat tacaagcgat ttatcgtcgt
     2581 gtctatgagt ttacagctga agaaaaacgt acattgactt ggcaagatgc tgtcgttgat
     2641 ctatcaaaag atagtataca aaaaggtgac gatacgattt ttctatccaa aacagaaatg
     2701 attatattag aaattcttat taccaaaaaa aatcaaatcg tttcgagaga tacaattatc
     2761 actgcattat gggatgatga agcatttgtt agtgataata cgttaacagt aaatgtgaat
     2821 cgtttacgaa aaaaattatc tgaaattagt atggatagtg caatcgaaac aaaagtagga
     2881 aaaggatata tggctcatga ataatttgaa atgggtagct tattttttga aatctcgcat
     2941 gaactggata ttttggatat tgtttttaaa cttgcttatg ttaggcatta gtctaatcga
     3001 ttatgatttt ccaatagaca gtttatttta tattgtttct ttgaatttaa gtttaacaat
     3061 gatttttctt atattgacat attttaaaga agtaaaatta tataagcatt ttgacaaaga
     3121 taaagaaata gaagaaatta aacataaaga tttagcggaa acgccatttc aacgtcatac
     3181 agttgattat ttatatcgtc aaatctcagc gcacaaagaa aaggttgttg agcaacagtt
     3241 acaattgaac atgcatgaac aaaccattac agaatttgtg cacgacataa aaacacctgt
     3301 gacagccatg aaattattaa ttgatcaaga aaaaaatcaa gaaagaaaac aggcattact
     3361 atatgaatgg tctcgtataa actcgatgct ggatacacag ctgtatatta ctagattaga
     3421 atctcaacgc aaagatatgt attttgatta cgtgtcactt aaacgcatgg tcattgacga
     3481 aatacaatta acaagacata ttagtcaggt taaaggtatt ggttttgatg ttgactttaa
     3541 agtggatgat tatgtttata cagatataaa atggtgtcgt atgattatta gacaaatttt
     3601 gtcaaacgca ttgaaatata gtgagaattt taatattgaa attgggacag aattaaatga
     3661 tcaacatgtt tcgttatata ttaaagacta tggcagaggt attagtaaaa aagatatgcc
     3721 gcgaatattt gaacgaggat ttacgtcaac ggctaacaga aatgaaacga cgtcttcagg
     3781 tatgggtcta tatttagtaa atagtgtaaa ggatcaatta ggtattcacc tgcaagtcac
     3841 gtcgactgtt ggtaagggga caactgtcag attgattttc ccattacaaa atgaaattgt
     3901 tgaacgcatg tcggaagtga caaatttgtc attttaaaca tgcgttttgt tacttagaat
     3961 tgatacatca atgcagcttc aacgttataa taagatagat gttagtcata tgttaaattg
     4021 aagatacaag tgccaaagcc taaaggaaat gaagttaaga taaattatag gagtgttaaa
     4081 gtggcaattt tagaagtaaa acaattaaca aaaatatatg gaactaaaaa aatggcacaa
     4141 gaagtgttgc gagatatcaa tatgtctatt gaagaaggcg agtttattgc tattatgggt
     4201 ccctctggat ctgggaaaac gacattatta aatgttttaa gttcaattga ttatatttca
     4261 caaggttcta ttacattaaa aggaaaaaaa ttagaaaagc tttcaaacaa ggaattatct
     4321 gatatacgca agcatgatat tggttttatt tttcaagagt ataatttact gcatacattg
     4381 actgttaaag aaaacataat gttaccacta acggttcaga agttagataa agaacatatg
     4441 ttaaatcgtt atgaaaaagt agcagaagca ttaaatatat tggatattag tgataaatac
     4501 ccttctgaat tgtctggtgg acaaagacaa cgaacatctg ctgcaagagc gtttattaca
     4561 ttaccttcta ttatatttgc tgacgaacca acaggtgcac tggattctaa aagtactcaa
     4621 gatttattaa aacgattaac aagaatgaat gaagcattta agtctacaat tattatggta
     4681 acgcatgatc ctgttgcagc aagttatgcc aatcgagtag tgatgctaaa agatggtcaa
     4741 attttcactg aattatacca aggggatgac gataaacata cctttttcaa agaaataata
     4801 cgtgtacaaa gtgttttagg tggcgttaat tatgaccttt aacgagataa tatttaaaaa
     4861 tttccgtcaa aatttatcac attatgccat ctatcttttt tcattaatta cgagtgtagt
     4921 attgtatttt agctttgtag cattaaaata cgcgcataaa ctaaacatga cagagtcata
     4981 tccaattatt aaggaaggct cacaagtcgg aagctacttt ctatttttca tcataattgc
     5041 atttttgtta tatgccaatg tgttatttat taaacgacga agttatgagc ttgcattata
     5101 tcaaacatta ggtttatcta aattcaacat tatttatata ctaatgctcg aacaattact
     5161 aatatttata attacggcaa tattaggtat tattattggt atttttggtt caaaactgtt
     5221 attaatgatt gtctttacat tattaggaat taaagaaaag gttccaatta tttttagttt
     5281 gagggcggta tttgaaacat taatgttaat cggtgtcgct tattttttaa cctctgctca
     5341 aaattttata ttagtgttca aacaatctat ttcacagatg tcaaagaata accaggttaa
     5401 agaaacaaat cataataaaa ttacatttga agaggttgtt ttaggcatct taggtatagt
     5461 attgattacc acaggatact atctatcttt gaacattgtt caatattatg attctatcgg
     5521 tatacttatg tttattttat tgtcaactgt gattggggca tacttatttt ttaaaagctc
     5581 tgtttctcta gtttttaaaa tggtgaagaa gtttagaaaa ggtgttataa gtgtaaatga
     5641 tgtcatgttc tcatcatcta ttatgtatcg tattaagaaa aatgcttttt cacttacggt
     5701 catggcaatc atttcagcga ttactgtttc agttctttgc tttgctgcta taagtagagc
     5761 gtccttatca agtgaaataa aatatactgc accacacgac gttacaatta aagaccaaca
     5821 aaaagctaat caattagcaa gtgaattaaa caatcaaaaa attcctcatt tttataatta
     5881 taaagaagta attcatacga aattgtataa agataattta tttgatgtaa aagcgaaaga
     5941 accatacaat gtaacaatta ctagtgataa atatatccct aatactgatt tgaaacgtgg
     6001 acaagctgat ttgtttgtag cggaaggttc tatcaaagat ttagtgaaac ataagaagca
     6061 tggtaaagca gttataggaa cgaaaaaaca tcatgttaat attaagttgc ggaaagatat
     6121 taataaaatc tattttatga cagatgttga tttaggtgga ccaacgtttg tcttaaatga
     6181 caaagactat caagaaataa gaaagtatac aaaagcaaag catatcgtct ctcaatttgg
     6241 attcgatttg aaacataaaa aagatgcttt agcattagaa aaagtgaaaa ataaagttga
     6301 taaatctatt aaaacaagaa gtgaagcgat aagctcaata tcaagtttaa ccggaatatt
     6361 attatttgta acatcatttt taggtattac attcttgatt gctgtatgtt gcattatata
     6421 cataaagcaa atagatgaaa ccgaagatga gttagagaat tatagtattt tgagaaagct
     6481 tggatttaca caaaaagata tggcaagggg actaaagttt aaaattatgt ttaattttgg
     6541 gttaccttta gttattgcac tatcacatgc atattttaca tcattagcat atatgaaatt
     6601 aatgggtaca acgaatcaaa taccggtttt catagtaatg ggattataca tttgtatgta
     6661 tgctgttttt gcagtgacgg cttataatca ttccaagcga acaattagac attccatata
     6721 aaatatacag atggctttca gtagagtagt ggattcggat tcacgaacta tactggaagc
     6781 tttttattat aaatgaagag aagttatatt tttagcatgt atagttgaat actgggttaa
     6841 aataccatat taataatgaa gtaaaggtat gagtgattat gaaagtgttt tgaatgaaat
     6901 atatttaatt ggtgatgctt ttaattgaaa agattaacag gattcaactt tgtaaattgt
     6961 attaaatgtg agaaaata
//
LOCUS       DAMZLE010000115         6902 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMZLE010000115 DAMZLE010000000
VERSION     DAMZLE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.116"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            45..159
                     /locus_tag="POB92_002127"
                     /pseudo
     CDS             45..159
                     /locus_tag="POB92_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            439..1098
                     /gene="arlR"
                     /locus_tag="POB92_002128"
     CDS             439..1098
                     /gene="arlR"
                     /locus_tag="POB92_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDI6579599.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1095..2450
                     /gene="arlS"
                     /locus_tag="POB92_002129"
     CDS             1095..2450
                     /gene="arlS"
                     /locus_tag="POB92_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDI6579600.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2734..5532
                     /locus_tag="POB92_002130"
     CDS             2734..5532
                     /locus_tag="POB92_002130"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDI6579601.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5546..6814
                     /gene="sucB"
                     /locus_tag="POB92_002131"
     CDS             5546..6814
                     /gene="sucB"
                     /locus_tag="POB92_002131"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDI6579602.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2607 a         1005 c         1257 g         2033 t
ORIGIN      
        1 aattatattg tttacatact ccttttgaat ttatacatta tgaaatgtta cttccaagtt
       61 caaaatcgca cattgaaatg atgtgtgaaa atgtttaaac tacggtcatt ttgtgaaaat
      121 aaagataata actattcatt ttacattagt gaaaagtaag tatacgacaa caattgatat
      181 tgcggtaagg ccttgtgtta cagtattcta tatttaagta ctgcaatcag aattaacaga
      241 attccattaa ctgattatta aatatttgag ttaataaata attaatgatt gtagcttgaa
      301 aaatttaaaa catggttatt gatttgtgat aaaatttaaa cgtaaacaaa ctaatttaaa
      361 aagcaactat tgtatagaaa aatacaaaat ttaaaatata ttaccttatt agaaaaagtc
      421 gtaatatgag gtgtacaaat gacgcaaatt ttaatagtag aagatgaaca aaacttagca
      481 agatttcttg aattggaact cacacatgaa aattacaatg tggacacaga gtatgatgga
      541 caagacggtt tagataaagc gcttagccat tactatgatt taatcatatt agatttaatg
      601 ttgccgtcaa ttaatggctt agaaatttgt cgcaaaatta gacaacaaca atctacacct
      661 atcattataa ttacagcgaa aagtgatacg tatgacaaag ttgctgggct tgattacggt
      721 gcagacgatt atatagttaa gccatttgat attgaagaac ttttagcaag aatacgtgcg
      781 attttacgtc gtcagccaca aaaagatatt atcgatgtca atggtattac aattgataag
      841 aatgctttta aagtgacggt aaatggcgca gaaattgaat taacaaaaac agagtatgat
      901 ttactatatc ttcttgctga aaataaaaac catgttatgc aacgggaaca aattttaaat
      961 catgtatggg gttataatag tgaagtagaa acaaatgtcg tagatgttta tataagatat
     1021 ttacgaaaca aattaaaacc atacgatcgt gacaaaatga ttgaaacagt tcgtggcgtt
     1081 gggtatgtga tacgatgaca aaacgtaaat tgcgcaataa ctggattatt gttaccacga
     1141 tgattacgtt tgtcacgata tttttgtttt gtttaattat tatttttttc ttgaaagata
     1201 cactgcataa tagtgagctt gatgatgcag aacgaagctc aagcgatatt aataatttat
     1261 ttcattctaa gcctgttaaa gatatatctg cattagactt gaatgcatct ttaggtaatt
     1321 ttcaagagat aattatttat gatgagcata ataataaatt atttgagaca tcgaatgata
     1381 acacagtgag agttgaacca ggttatgaac accgttattt tgaccgcgta ataaaaaaac
     1441 gctataaagg cattgaatat ttaattatta aagaaccaat tacaacgcaa gatttcaaag
     1501 ggtatagctt gttaattcat tcactagaaa attatgataa catcgtaaaa tcattgtata
     1561 tcattgcgct ggcatttgga gtgattgcaa caattataac tgccacaatc agttatgtat
     1621 tttcaacaca aattactaaa ccgcttgtca gtttatcaaa taaaatgatt gagattcgac
     1681 gagatggttt tcaaaataaa ttgcaattaa atacaaatta tgaagaaata gataatttag
     1741 caaatacgtt taatgagatg atgagccaaa ttgaagaatc atttaatcaa caaagacaat
     1801 ttgttgaaga tgcgtcacat gaattacgaa caccattaca aattattcaa ggtcatttaa
     1861 atttgattca gcgatgggga aaaaaagacc cagcagtatt agaagaatcg ttaaatattt
     1921 ctattgaaga aatgaatcgt atcataaaat tagtcgaaga attacttgaa ttgactaaag
     1981 gagatgtaaa tgacatttct tctgaagcac agaccgtgca tattaatgat gaaattcgct
     2041 cgcgaataca ctcattaaaa caattgcatc ctgattatca atttgatacg gatctgacat
     2101 ctaaaaatct agaaattaaa atgaaacctc atcaattcga acaattattt ttaatcttta
     2161 ttgataatgc aatcaaatat gatgtgaaga ataagaaaat taaagttaag acaaggttaa
     2221 aaaataagca aaaaataatt gaaattacag atcatggaat tggtattcca gaggaagatc
     2281 aagatttcat ttttgatcgc ttttatcgag tggataaatc tcgttcaaga agtcaaggcg
     2341 gtaatggact cggattatct attgctcaaa aaatcattca attaaacgga ggatcgatta
     2401 aaattaaaag tgaaattaac aaaggaacaa cgtttaaaat catattttaa tcatgactga
     2461 gacgtcaatc aaagtcatag gatcaatttt ttaagtacac attagctgtg actaatgtat
     2521 aagaacaact ataaaacaaa taaacagtgg ttctttatca tttctgttgt actcccaaaa
     2581 tttacaataa aatacatcta taaacctaga agaatcaacg cttttgttga ttcttctttt
     2641 tagcagataa ataggtaaat ctactttaac aaataactaa atagtgatat tattacattg
     2701 taagcgtttc aacatttttg tggagggtgt aaaatgacta acgaaagaaa agaagtttca
     2761 gaggctcctg taaacttcgg tgcgaattta ggtctaatgt tagatctata tgatgacttt
     2821 ttacaagatc catcatctgt accagaagat ttacaagtct tattcagcac aattaagaat
     2881 gatgactcaa ttgtaccagc tttaaaaagt acaagtagtc aaaatagcga cggcacaatt
     2941 aagcgtgtca tgcgtttaat tgataatatt cgccaatacg ggcatcttaa agccgatatt
     3001 tatcctgtaa atcctccaaa aaggaaacat gtacctaaat tagagattga agactttgat
     3061 ttagatcaac agactttgga aggtatatca gcaggaattg tttcagatca ctttgccgac
     3121 atttatgata atgcttatga agcaatttta agaatggaaa aacgttacaa aggaccaatt
     3181 gcatttgagt atacacatat taataacaat accgaacgtg gttggttaaa aagaagaatt
     3241 gaaacgccat ataaagtaac gttaaataat aacgaaaaaa gggcactatt caaacaatta
     3301 gcgtatgttg aagggtttga aaaatatctt cataaaaact tcgttggtgc aaagcgtttt
     3361 tcaattgaag gggtagacgc acttgtaccg atgttacaac gtactattac gattgctgcg
     3421 aaagaaggta ttaaaaatat acaaataggc atggctcacc gtggacgttt aaacgtttta
     3481 acgcatgtct tagaaaaacc gtacgaaatg atgatttcag aatttatgca tacagatcca
     3541 atgaaattct tacctgaaga tggtagcttg cagttaactg ctggatggac tggtgatgtg
     3601 aaatatcacc ttggtggcat taaaactact gattcatacg gtacaatgca gcgtattgca
     3661 ctggctaaca atccaagtca cttggaaatt gttgcacctg ttgttgaggg gcgtacgaga
     3721 gcagcacaag atgatacaca acgagctggg gctccgacga ctgatcatca taaagcaatg
     3781 ccaattatta tacatggcga tgctgcttat cctggtcaag gaattaactt cgaaacaatg
     3841 aacttaggaa acttgaaagg ctattctacg ggtggttcat tgcatattat tactaacaat
     3901 agaattggat ttactacaga accaattgat gcacgttcaa caacttattc tacagatgtg
     3961 gccaaaggtt atgatgtgcc aatattccat gtcaatgcag atgacgttga agctactatt
     4021 gaagcaattg atattgcaat ggaatttaga aaagagtttc ataaagacgt cgttattgat
     4081 ttagtaggtt atcgtcgttt cggacataac gaaatggatg aaccatcaat tactaatcca
     4141 gttccttatc agaatattcg caaacatgac tctgttgaat atgtgtttgg taaaaagctt
     4201 gttaatgaag gtgtcatttc agaagatgaa atgcattcat ttatagaaca agtccaaaag
     4261 gaactaagac aagctcatga taaaattaat aaagctgata aaatggataa tccagatatg
     4321 gaaaagcctg cagatcttgc attaccgtta caagcagacg aacaatcatt tacttttgat
     4381 cacttgaaag aaataaatga tgcattgtta acatatccgg atggctttaa cattttgaaa
     4441 aagttaaaca aagttcttga gaagcgtcat gagccgttta ataaagaaga tggtttagtt
     4501 gattgggcac aagcagaaca acttgcattt gcgacaattt tacaagatgg tacaccgatt
     4561 cgcttaactg gtcaagatag tgaacgtggt acattcagtc ataggcatgc cgtgttacat
     4621 gatgagcaaa caggtgaaac atatacacct ttacatcatg ttcctgatca aaaagcgaca
     4681 tttgatatac acaattctcc gctttcagaa gcagcagtag ttggttttga atacggctat
     4741 aatgtggaaa acaaaaaaag cttcaatatt tgggaagcac aatatggtga ttttgcaaat
     4801 atgtcacaaa tgatttttga caacttctta ttcagttctc gctcaaaatg gggagaacgt
     4861 tcaggattaa cattattctt acctcatgca tatgagggtc aagggcctga acattcatca
     4921 gcaagattag agcgattttt acaattagct gctgaaaata attgcacagt tgtcaactta
     4981 tctagttcaa gtaattattt ccacttattg cgtgcacaag cggctagttt agattctgaa
     5041 caaatgcgac cattggttgt tatgtcacca aaaagcttac tgagaaataa aacagttgca
     5101 aaaccaattg atgaatttac ttctggtgga tttgagccaa ttttgacaga atcatatcaa
     5161 gcggataagg ttacaaaagt tattttggca actggtaaaa tgttcattga tttaaaagaa
     5221 gcattagcta aaaatccaga cgaatcagta ttactcgttg cgattgaaag attgtatcca
     5281 ttcccagagg aagagattga agcattacta gcacaattgc caaaccttga agaagtgtca
     5341 tgggtacaag aagaacctaa aaatcaaggt gcctggttat atgtctatcc atatgttaaa
     5401 gtgctagttg cagataaata tgatttaagt tatcatggca gaattcaaag ggctgctcca
     5461 gctgaaggcg atggagaaat tcataaactt gttcaaaata aaattataga aaatgcatta
     5521 aaaaataact agggggaaat aagtcatgcc agaggttaaa gttccagaat tagcagaatc
     5581 tattacagaa ggtaccattg cagaatggtt gaaaaacgta ggggatagcg tagaaaaagg
     5641 tgaagctatt cttgaattag aaactgataa agttaatgtc gaagttgtat ctgaagaagc
     5701 aggtgtatta tctgaacaac ttgcaagtga aggcgacact gtagaagttg gacaagcaat
     5761 tgctatcatc ggcgaaggta gtggcaatgc ttctaaagaa aatagtaacg acaatactcc
     5821 acaacaaaat gaagaaacaa ataataaaaa agaagaaaca acaaataatt cggtagataa
     5881 agctgaagta aatcaagcaa atgatgacaa tcagcaacgt attaatgcta cgccttctgc
     5941 gcgtcgatat gctcgtgaaa atggtgtgaa tcttgctgaa gtaagtccga aaacaaatga
     6001 tgtggttcgt aaagaagata ttgataagaa acaacaggca ccggcatcaa cacaaacaac
     6061 acaacaagca tctgcaaaag aagagaaaaa atacaatcaa tatcctacaa aaccagtgat
     6121 tcgtgaaaaa atgtcacgta gaaagaaaac agctgccaaa aaattattag aggtatctaa
     6181 taatacagct atgttaacaa catttaacga agttgacatg acaaatgtta tggaattgcg
     6241 taaacgtaag aaagaacaat ttatgaaaga tcatgatggt actaaattag gatttatgtc
     6301 attctttact aaagcttctg tagcagcttt gaaaaagtat ccagaagtta atgcagaaat
     6361 cgacggcgac gacatgatta cgaaacaata ttatgatatt ggtgtagctg tttctacaga
     6421 tgatggatta ttagtaccat ttgtaagaga ttgtgataaa aagaattttg cagaaatcga
     6481 agcagaaatt gctaatttag cagttaaagc acgagagaaa aaacttggct tagatgatat
     6541 ggttaatggt tcatttacga ttacaaatgg cggtattttt ggatcaatga tgagtacgcc
     6601 aattatcaat ggtaatcaag ctgcaatctt aggcatgcat tcaattatta caagaccaat
     6661 tgcgattgat caagatacaa tcgaaaatcg tccaatgatg tatattgcat taagctatga
     6721 tcatagaatt attgacggta aagaagcagt tggattctta aaaacaatta aagaattaat
     6781 tgaaaaccca gaagacttat tattagaatc ttaatccaca acacaaaata gtttactatt
     6841 cctaaaagcg ggattaaatc aataacaaac agcagtaaga ttatttccta gtcgaaatat
     6901 ct
//
LOCUS       DAMZLE010000116         6826 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMZLE010000116 DAMZLE010000000
VERSION     DAMZLE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.117"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..550)
                     /gene="icaR"
                     /locus_tag="POB92_002132"
     CDS             complement(<1..550)
                     /gene="icaR"
                     /locus_tag="POB92_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDI6579603.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIF"
     gene            714..1952
                     /gene="pgaC"
                     /locus_tag="POB92_002133"
     CDS             714..1952
                     /gene="pgaC"
                     /locus_tag="POB92_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDI6579604.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESDTIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            1916..2221
                     /gene="icaD"
                     /locus_tag="POB92_002134"
     CDS             1916..2221
                     /gene="icaD"
                     /locus_tag="POB92_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDI6579605.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2218..3090
                     /gene="icaB"
                     /locus_tag="POB92_002135"
     CDS             2218..3090
                     /gene="icaB"
                     /locus_tag="POB92_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDI6579606.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLIKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3077..4129
                     /gene="icaC"
                     /locus_tag="POB92_002136"
     CDS             3077..4129
                     /gene="icaC"
                     /locus_tag="POB92_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDI6579607.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4575..6617)
                     /gene="lip1"
                     /locus_tag="POB92_002137"
     CDS             complement(4575..6617)
                     /gene="lip1"
                     /locus_tag="POB92_002137"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDI6579608.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT         2124 a         1117 c         1039 g         2546 t
ORIGIN      
        1 aaaatatatt tagtagcgaa tacacttcat ctttgaattg acttttactt tcttccactg
       61 ctccaaattt ttgcgaaaag gatgctttca aataccaact ttcaagaaac agcaatatta
      121 aattctgaaa gtcttctttt gtcatcttta tctttgattc atcatagaat tttgctatct
      181 ctttacttaa tgattgattt aaatcttgta tttgtccgta aatatttcca gaaaattcct
      241 caggcgtatt agataattga acgtacattc taatatacct ttcttcgatg tcgaaaataa
      301 actcaaataa gaattgatat aaagcatcaa ttgaatagtt cgatttattt tgattcatca
      361 taataatatt attaaggtaa tcaaaacaac atttaacact ttgttcgtaa atactttttt
      421 tcgagtcaaa atggtaatat aaactcgctt tctttatatt tacactttta gctatatcat
      481 caagtgttgt accgtcatac cccttctctg aaaataaggt tattgcgtta tcaataatct
      541 tatccttcaa tttttataac cccctactga aaattaatca cactatgtta caggaaaatt
      601 aagttgcaat tacaaatatt tccgtttaat tataacaaca atctattgca aattaaaata
      661 ctatcaatta ccatatggct tacaacctaa ctaacgaaag gtaggtaaag aaattgcaat
      721 tttttaactt tttgcttttt tatcctgtat ttatgtctat ttactggatt gtcggttcaa
      781 tttatttcta ttttaccaga gaaattagat attcattgaa caagaagcct gacataaatg
      841 tggatgaatt agaaggcatt acatttttac ttgcctgtta taacgaaagt gacacgattg
      901 aagatacgtt gtctaatgtt cttgcactca aatacgagaa gaaagaaatt attatcatta
      961 atgatggaag ttcagataat acagcagaac tcatctataa aatcaaagaa aataatgact
     1021 ttattttcgt cgatttacaa gaaaacagag gtaaagccaa cgcactcaat caaggcatta
     1081 aacaggcttc atatgattat gtaatgtgct tggatgcaga tactatcgtt gatcaagatg
     1141 caccatatta tatgattgag aatttcaaac atgatccaaa acttggtgca gttacaggta
     1201 atcctagaat tcgaaataag agttctattt taggtaaaat tcaaacgata gaatatgcaa
     1261 gtttaattgg ctgtattaag cgaagtcaga cacttgctgg cgcagtcaat actatttcgg
     1321 gtgtcttcac tctatttaaa aaaagtgcag ttgtcgacgt tggctactgg gatactgata
     1381 tgattaccga agatattgca gtttcttgga aattgcattt acgtggatat cgtattaagt
     1441 atgaaccgct tgccatgtgt tggatgttgg ttccagaaac attgggaggt ctttggaagc
     1501 aacgcgtgag atgggctcaa gggggacacg aagtattact acgagacttt tttagcacaa
     1561 tgaaaacgaa aaggtttcct ttatatattt tgatgtttga gcaaatcatc tcgattttat
     1621 gggtatatat agtgcttcta tatttaggct atttgttcat aacagcaaac ttcttagact
     1681 atacatttat gacatatagt ttttcaatat ttctactatc atcatttact atgactttta
     1741 taaacgttat tcaatttaca gtcgcactct ttattgatag tcgctacgag aaaaagaata
     1801 tggctggact catatttgta agttggtatc cgacagtata ctggattatt aacgcagcag
     1861 tagttcttgt cgcatttcca aaagcattaa aacgtaagaa aggtggttac gcaacatggt
     1921 caagcccaga cagagggaat acccaacgct aaaatcatcg ctaaatattg taagagaaac
     1981 agcacttatc gctatatctt gtgtcttttg gatatattgt ttagttgttc tactcgttta
     2041 tattggtact atatttgaaa ttcatgacga aagtatcaat acaatacgtg ttgctttaaa
     2101 cattgaaaat actgaaattt tagatatatt tgaaactatg ggcattttcg cgattatcat
     2161 ttttgtattt tttacaatta gcatattgat tcaaaaatgg cagagaggaa gagaatcgtg
     2221 aagtatagaa aatttataat tttagtgttg agtatcttga tcatattgcc tgtaagcaca
     2281 ctggatggtc atcatattgc aaatgcagat gacgattcac ctaaaaaact gaaatataaa
     2341 gaaaatagtg ctctggcatt aaattatcac cgtgtaagaa aagcgaattt tctgaataat
     2401 tttatttact tcttttctag tagtaaagaa attaaaaatt atagtgttag tcaatcacaa
     2461 tttgaatctc aaataaaatg gctaaaatca catgatgcta aatttttaac cttgaaagaa
     2521 tttttatatt acaagaaaaa aggtaagttt ccaaaacgaa gtgtatggat taactttgat
     2581 gatatggatg aaactattta tgaaaatgct tatccaatct taaaaaaata taaaataccg
     2641 gcaactgggt ttattatcac aggtcatgtt ggggaagaaa actttcacaa cctcgatatg
     2701 attagtaaaa aagaactaaa agaaatgtat aaaactgggt tatgggaatt tgaaacacat
     2761 acccacgatt tgcataactt atctaaaaat aataagtcaa aattaataaa agcttctgaa
     2821 gctacaatca taaaagattt aaacaaaagt gaaaaatatc taactaaaaa ctttaaaaag
     2881 tcgcagaaaa ctatagccta tccttatggc ttgatgaatg acgataaatt accggtaatc
     2941 aaaaaagctg ggttaaaata cggtttttca ttagaggaaa aagcagtcac tccgaactcc
     3001 aatgattatt acatccctag aatattaatt agtgatgatg cttttgagca tttaattaag
     3061 agatgggacg gattccatga aaaagattag acttgaactc gtatatttac gtgctattat
     3121 atgtgcaatt attattatca cacatttact tacacaaatt actttaaaac atgaaaatat
     3181 ggagggtgga tccttagtgt tacaatttta cattcgtaat attgtgattt ttggtacacc
     3241 ttgctttatt atcttgtcac agttactgac aaccttgaat taccaaaaag tcacctatag
     3301 atacttaact acacgcgtaa aatatatact tattccttac atattaatgg gattgtttta
     3361 cagttatagt gaatcattat taacagattc aagtttcaat aaacaattca ttgaaaatgt
     3421 cctattaggt caatggtatg gctattttat cgttgttatc atgcaattct ttattttgag
     3481 ttatatcatt tttaaaatta actataacct attcaacagt aaaatattat tattgttatc
     3541 ttttatttta cagcaatcat ttttatatta ctttacgaac aacacagcgt ttcacgatac
     3601 cgtgctacac tattatccat taagtgaaaa tactataata ttcggatgga ttttttattt
     3661 cttcttaggt gcatatatgg gttataacta cgaacgtgta ttaaatttct tagaacgtta
     3721 tttagttatt atgattgtat tagctgtagc tacttatttt gtgtttattg cgttagcaaa
     3781 tggagactat tggaacgtta ccagcttttc atattcatta acaccatata atagtattat
     3841 gtttattgtt atcttgggta tttgcacgca ttttaaaaca atgttattta atacgattca
     3901 aatgattagt gctttctcat tctttattta tttattacat ccaatcattc tagactcatt
     3961 gtttgcatat acaaatatat ttgaggataa tacaatggtc tttctagcga tatcactact
     4021 attcatttta ggattatgta taggtgtcgg catgatattg cgtgaattct atatctttag
     4081 gtttattatt ggaaaacaac catataaatt gaacattaat gcttattaat tattaagcta
     4141 tgttaaaaac acgcggtggg cgaaatcagt ttgaattgac tgacttcgtt ttaccgcgtg
     4201 tttaatattg ttatacatat attctaattg cacatttaaa cttcgtaaat gccaatggga
     4261 gtgggacaga aatgatattt tcgcaaaatt atttcgtcgt cccaccccaa cttgcacatt
     4321 attgtaacct gactttccgc cagcttctat gttggggccc cgccaacttg cacattattg
     4381 taagctgact ttccgccagc ttctttgttg gggccccgcc aacttgcatt gtttgtagaa
     4441 tttcttttcg aaattcttta tgttggggcc tcgccaatgt tttacttgaa taattctttt
     4501 agaattctaa ataatgatcc gattaattga aagaagtctg cagtcattat taattcctcc
     4561 ctttacttta taaattatgc ttgcttagta tcagtcagct tttcagtttt cactaaatcg
     4621 tctgctaaat gatgccaaaa atcttgtaat tcttctcttg tgcgcactgt atcagaactg
     4681 tcttgtccta caaagtcaac atgatcccaa tcatgttttg taggcgtcac ttgccaaatg
     4741 cctttttgaa ttttatctgt cgcttttgta taagcttgat taaatggatg ttgagaagaa
     4801 ataacggata ctaaaccatc gttttctcgc cattcttttt cagtagcttt accgattaag
     4861 ttaccagtaa tcacaaatgg gaaaaacata tttaagtctg ctttttgtct atcgctattt
     4921 aatgctttgt gcgttgcttc accagtgtat gttttataca caatgttagg gttcaacgac
     4981 gttttacgat ttaaatctgt tgcaccctca cgcgtcagat cgtaaaatcc attatctttt
     5041 gatttccata aattagattg tttaacgcgt ttgacataat caatatatga ttcatttggc
     5101 ttctgtttta gaccccattg agccaacccg aagtctactc ttgaattttt attaccaaac
     5161 attttaccga tatcaaatac gatttgtctc actaaagctt cattaccagc taaatctgat
     5221 gcgtgtgtac cattatgtgg tgttcctaaa gtagtaattg atgaaatcat attgtcatga
     5281 ttacctttga atagtggaga aatttcgcca ccatgtttct tttgatactc tatttcttca
     5341 cgattaccat tacgcagtaa ttcttctagt tgacgtatcg tttgaccgcc catactatgt
     5401 ccaactaggt gtaccttctg tcctggtttc cagtctttgt aaattccttc gtatgttttt
     5461 ccataacgtt catgtccata ttttgctgca tgtgctgcac cataatctac acgaccgcct
     5521 ttgatataat aataaagttc aactgcgcgg tcatagttac ttccaaaagc acttatactt
     5581 gcttcataag ctttgtaacc attttcttct aaatcttggc gaatgttcat tttattaccg
     5641 ccccaataat gagctaacac tgaaggatta atatcatctg taaacccatt gaaaccatgc
     5701 actaaaacga taggatcctg atttttatac tgcccttgtt tggcaacttt attcgtttga
     5761 tcatctttcg cttttgcagt aagttccttt gtccctttgt ttggtaacgt tgctactgca
     5821 ctacttttta ataaatttaa tccatttttc ttgtcattgt cctttgttgg ttctactggt
     5881 gatttttgat tgttttttaa atctgaaact gatagtgttt tttcgtcttt ttgaggtttc
     5941 acttcttgtt cattgttaaa attgtagtta gtaattttat cattcgcttg attattttgt
     6001 tgtggttgta ctttctctat ttctgtctca ttaacaattt ctcgttgact tgtttgatct
     6061 aacttattta atgatggtgc ttcctttatt cgactttgtg aaggtagatt gttattagcg
     6121 tttgaatctt gactattatc tgcctctttt gactgttgct gtgacgccac tttactttgt
     6181 ttaccatcat taatagcctg ttctaaccct tgatttacat cgctaggatt agcattgtta
     6241 ttttttgttg tatcacgttg aatattttta ttattaagcg atgatttttt atcatccttt
     6301 agttgatctt tattcaaatc atctgtttta cgatgatttt cttctgatat tgttttatca
     6361 ttatgcaagt tcttagttaa attttcagta tgttgctgtt cgtcaacacc gttttcacgt
     6421 tgataattag cattttccct agtttgttga tccccaatag attgtgctgt aactgtatcc
     6481 tcctgtgaat ttcccatatt cacttgcttc tcagctgctt gtgcttgtcc accacttaaa
     6541 aatagtaatg tagctattaa aatggaagat gcacctacac taaatttacg aatactatac
     6601 ttattttgac ttttcatcat tgtcagcacc tctatttttt tattttagat aaacaaattg
     6661 ataattattc gaggaataaa ctcaaataat ttacttaacc aagcactcat aatgaccatc
     6721 ctttctattg tttaatttga cgagaaagcg tttacatttg tatttaaaaa atatattctc
     6781 caaagagaat ttaaagtgta tacgctaatc atttcaaatt attaat
//
LOCUS       DAMZLE010000117         6807 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMZLE010000117 DAMZLE010000000
VERSION     DAMZLE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.118"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            32..565
                     /locus_tag="POB92_002138"
                     /pseudo
     CDS             32..565
                     /locus_tag="POB92_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..2168)
                     /locus_tag="POB92_002139"
     CDS             complement(678..2168)
                     /locus_tag="POB92_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDI6579609.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNETTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            complement(2360..2581)
                     /locus_tag="POB92_002140"
     CDS             complement(2360..2581)
                     /locus_tag="POB92_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDI6579610.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            complement(2581..3081)
                     /locus_tag="POB92_002141"
     CDS             complement(2581..3081)
                     /locus_tag="POB92_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6579611.1"
                     /translation="MFKKLFGKGKEVQKDIAIYTPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            complement(3093..3521)
                     /gene="msrB"
                     /locus_tag="POB92_002142"
     CDS             complement(3093..3521)
                     /gene="msrB"
                     /locus_tag="POB92_002142"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI6579612.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            complement(3514..4047)
                     /gene="msrA"
                     /locus_tag="POB92_002143"
     CDS             complement(3514..4047)
                     /gene="msrA"
                     /locus_tag="POB92_002143"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6579613.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            complement(4133..4972)
                     /gene="fakB2"
                     /locus_tag="POB92_002144"
     CDS             complement(4133..4972)
                     /gene="fakB2"
                     /locus_tag="POB92_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDI6579614.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            complement(4987..5466)
                     /locus_tag="POB92_002145"
     CDS             complement(4987..5466)
                     /locus_tag="POB92_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI6579615.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVVGVDVIHSIEDIYQLPGHVFIFGGQILFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            complement(5666..6622)
                     /locus_tag="POB92_002146"
     CDS             complement(5666..6622)
                     /locus_tag="POB92_002146"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6579616.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
BASE COUNT         2110 a         1202 c          951 g         2544 t
ORIGIN      
        1 tgtaatttta cttttaaata ctttaaaaaa aataagacac tttgcccaac ttacactacc
       61 aatagaaact tctgttagaa ttcctcaaaa tgatatttca cgatatgtta atgaaattgt
      121 tgaaacgata cctgatagcg aattcgatga attcagacat catcgtggcg caacatccta
      181 tcatccaaaa atgatgttaa aaattatctt atatgcatac actcaatctg tattttctgg
      241 tcgtagaata gagaaattac ttcatgacag tattcgaatg atgtggttag ctcaaaatca
      301 aacaccttct tataaaacta ttaatcgttt tagagtaaat cctaatactg atgcattaat
      361 tgaatcttta tttatccagt ttcatagtca atgtttaaag caaaatctta ttgatcataa
      421 ttcaattttt attgatggta caaaagtaga agctaatgcc aataggtata catttgtgtg
      481 gaagaaaagt attccaaatc acgaatcgaa attgaacgaa aattcaaaaa cactatatcg
      541 tgacttagtt gaagaaaagc tgatttctat caaataatta atagaaatca gcttttttac
      601 attgcctaag aacttaatgt tccagcctga gcgtgtcatt aacataataa taatacaatt
      661 ttagcagtgt gtatctctta ttttaaaata ttaatcaatt tatcgagaac atcatcatgt
      721 ttattagctt tttcaactaa taactcagta aatttattat tcgtttcttt attaaattca
      781 ccagttacct cgagtttatt cgcttgttgg aaagctttaa cttgattttc taaagcttga
      841 tcaaattgcg ttgtttcatt atcaacttta taacctaaag ctgacaaacc aattttaata
      901 gttttaatat ttttatcatc gtctccaact ttaaatgttt tcgtattagg aatgacattt
      961 aaagattgat atttaggtgt gtcaatagta acgtctggtt taatgccttt accgtgaata
     1021 taatgaccat ctggcgttaa ccatttcatt tcagtatatt ttaacaatga accatcctta
     1081 aactctcttg tagtttgtac gacacctttg ccgaatgttt ttgacccata aactttagct
     1141 ttattatagt cttttagcgc accagtaaac acttcagaag cgctagctga accttcattc
     1201 actaagatgg atatatccat gtctttcgct tcttttaacg catcattaga agtttgaatt
     1261 gcttcagtat ctttaccttt ttctagttta acaacagttt ttcctttatc gataaaaata
     1321 tttgccattt taacagcttc atctagtagt ccacctggat tatttcttaa atctaaaaca
     1381 atctttttca aaccatcttt gtgagctttt agaactgcat ctttcaattc acctgatgta
     1441 tcattctgga atttattaat agtaataact ccaactttac cttttttctt atactcaaca
     1501 cttttaacat gaattttttc acgtttaatc ttaacgtctt tttcttcact acctcgttga
     1561 acagttaaag tgacttcagt gttttcttta ccacgaacat ctttgacaac ttcatctaat
     1621 gcttttcctt taattgattt tccatttact ttagtaatga catctttagg acgaatgcca
     1681 gcacgttctg ctggagatcc cttcatagga ctagtaacca taatttgatc atttttcttt
     1741 tgcatttctg caccaatacc tacaaaatca cctgaaacac cttcattaaa ggatttcgtt
     1801 tgttcttttg ttaaatattc agaataagga tcttttaatt ctttgaccat gccatcaatt
     1861 gcagctttac ttaacttgtc agagtcctgt tttttgtaat aatcactatt taagatttta
     1921 tacacatttt caattttatt taagtttgct tgatcagttt tgtttaaacc acttattttt
     1981 tgatttataa aaatatatgc aacaactgtg atgacagctg ttattaggat tgtaccaatt
     2041 aatattgata tgaattgcca acgttttaag tgaacgcgtt tcgatgaatt agtttcttgg
     2101 tcttgattgc ttgttgcaat ttcagcgcgt tcatcatcgg atgaagatgt gtgttgctta
     2161 tcatccattg tatcacttcc ttattatgta ttatgtactt ttaaattctg tgtagttaat
     2221 catatcaatt tatgcaagat agcgcaccat atttcactat aattgattaa tttttagtca
     2281 caacttagtt tattttaaat taaaaccagg ataacgaatt gcatcaccct ggttctaact
     2341 attattatta aaaattatat taaaatttta gccattccgt atattcttca tataaatcat
     2401 caaatacaga cattggcaat gtgaaatcac catgtgtctc aatataatca gacagtatgt
     2461 taaaatcatc atcgtgtttt gggaaagcaa gatcgtcaaa tatctcttct gctagacgac
     2521 ctttatcgtc gtgtcgacca cgaactgtca tgacaaattg ataaaacgaa tagtttttca
     2581 ttagttcatt gtcacatcaa tcacttttgt ttcaccttta atcacagcat tttcatcata
     2641 aatattaatt gaagctgctt gatcagtgtt agtaattata attggtgaaa ttacagattt
     2701 agcgttatta ttaatatatt caaggttgaa tcttactaat ggatctccga cgttaacttc
     2761 gtcaccacta gacactaaca cttcaaatcc ttcaccgtct aattgaactg tgtctaaacc
     2821 gatatgaact aataattcta atccgttatc tgcttttaac ccaatcgcat gcttagttgg
     2881 aaagacattg tcaacacgtc ctgcaattgg agacacaact tctccttcag ttggattaat
     2941 accaaaacct tcgcccatca ttttttgtgc gaatacagga tctggaatat cttcaatttt
     3001 cacgaattct ccagttaatg gtgtataaat tgcgatatct ttctgaactt ctttgccttt
     3061 tccgaataat tttttaaaca tactttccac tcctacttat caaaatgtga tattaaatcg
     3121 ccataaccca attcttctaa cttttcatat ggaataaatt gaattgcagc ggaattgata
     3181 cagtatctta agccgccact ttctttaggt ccatcattaa agacatgtcc taaatgacta
     3241 tttgattctt ctgaacgcac ttcagttctc aacataccaa atgatttgtc gactaattct
     3301 ataatttcat cgtcatcaag cgctttggaa aagctaggcc atccacattc agaatgaaac
     3361 ttttcttcag atgtaaataa aggtttacca gaaattttat ctacataaat tcctttagca
     3421 aaatgattcc aatattcatt cataaatggt ggttcagtgc cgttttcttg tgtaacaata
     3481 tattctatat ctgttagttc acttttatct tttttaagca ttttgattcc cccaatgtga
     3541 ttctataaac gcttttctac ctgaaccacg ttgatattgg taataatgta ccgggttctt
     3601 tttgtaataa tcttgatggt agtcttcagc tggatagaaa tttttatatg gtttaatagg
     3661 tgtaatcact ggtttcttga aaataccttg ttcatttaat tgttgctttt taaactcagc
     3721 agcctttttc tgatgttcat catgatagaa aatgactggt tgatagcttt cgcctctatc
     3781 gaaaaattgc ccttgatcat cagttgggtc aaatgttttg aaatatatgt ctaatatatt
     3841 ttcaaaggaa gtaacctctg gatcaaacgt aatttgtact gcttcgacat ggccggtttg
     3901 attcgtacat acctgttcat aagttgggtt gtcaacatga ccgccactat aaccagatac
     3961 gactgacttg atgcctggat atgatgtaaa tggtttaacc atgcaccaga aacatcctcc
     4021 tgctaatgtt gcatattctt ttgtcatatg aattacctcc tctatctatc taattataaa
     4081 tttagtctat aaaggattga aatggaagta attaacactg aaaagattaa atttacttct
     4141 taaggactac gaggccaatc gcaccttgac cagtatgtgc agaaataact ggtgtagtta
     4201 catttatatc gtaattattc acatgaaaag cttcattaaa aactttcttc aatttatcaa
     4261 catattcaat gacgttagca tgtgcgacac caatggattt gatttcatga tctcctataa
     4321 attcagcaat ttcctttttc aagtattgga tactagaatt ttgagttctc gcattgtgca
     4381 caagctctaa gcgaccatca tctagtgtac caattggttt aattttcata agattaccaa
     4441 tcaaaccttt tgttttacta attctgccac ctttaattaa ttgattcaat tgccctataa
     4501 ctacaaataa tttaatgttt tctcttaaat gatttaactt tttaactatt tcagaagttg
     4561 agacaccttc ttttacaagc tctactaggt gttgtatttg ataccctaaa ccaaaagaaa
     4621 tagattttga atcaataaca gttacattag catctaccat ttgacttgct tggtaagcag
     4681 tgttatatgt accacttaat cctgaagaaa gatgaatact tatgatttca gagccatctt
     4741 ttcctagttc ttcataagca gatataaatt cacctatggc tggctgactt gtctttacat
     4801 cttcatcatt ttcaatatga ttaataaatt cttctgatgt aatatctact tggtcaacgt
     4861 atgaagctcc ttcaatagtt aaacttaaag gaattacatg aatgttgttt gcttctaagt
     4921 attctttaga taaatcggat gttgagtctg ttactataat ctgttttgtc atggtcgttt
     4981 tcccccttat tttttacgaa ttaaatgtag aaaggtatgt ggaattgtat ttttctcatc
     5041 tagtttacct tcaactgaag aggcaacttc ccagtcttca aatgtataag gtggaaagaa
     5101 cgtatcacca cggaatttac cttcaataac agtaatatac atgtcgtcca ctttatcaat
     5161 catttcttca aataatattt gccctccaaa tatgaaaaca tggcccggta gttggtaaat
     5221 atcttcaata gagtgaatta catcaacgcc cactacgttg aaacttgtat ctgaagtaag
     5281 tacaacattt cgacgattcg gtagtggttt accaatcgat tcaaatgtct tacgacccat
     5341 tactaaagta tgacctgttg ataatttttt aacatgtttc aaatcatttg gtaggtgcca
     5401 aggtaattga ttttcaaaac caattactcg ttgcaagtca tgtgcaacta gaatggataa
     5461 agtcataatt atcctccttc ttctatcatt tcatttttta ttactaagtt atctttaatt
     5521 taacacaatt tttatcataa agtgtgatag aaataatgat tttgcataat ttatgaaaac
     5581 gtttaacaca aaaaagtact tttttgcact tgaaaatact atgatgtcat tttgatgtct
     5641 atatggttag ctaactatgc aatgactaca ctgctattgg agcttttatt gctggatgtg
     5701 attcatagtc aacaatttcc aaatcttcat aatttatgtc gaaaatagac ttgtcactgt
     5761 taatttttaa tgttggagga ttgaagcttt cacgtgctaa ttgtgtttga atcgcatcaa
     5821 tatgatttga ataaatatgt gcatctccaa atgtatgcac aaattctcct acttcaagtc
     5881 cacattcttt ggcaataagg tgtgtcaata aagcgtagct tgcgatatta aatggcacac
     5941 ctaaaaagat atctgcgcta cgttggtata actggcaact taacttacca tcttggacat
     6001 aaaactggaa catggtatga caaggcggaa gtgccattgt atcaatttct gttggattcc
     6061 atgcagatac gatgtgtcgt cttgaatctg gattatgctt aatttgttca attactgttt
     6121 taagttgatc aaaatgatta ccatctttat caacccaatc tcgccattgt ttaccataaa
     6181 catttcctaa atcaccgaat tgcttcgcaa aagtatcatc ttcaagaata cgttgcttaa
     6241 attgtttcat ttgttcttta tattgttcgt taaattcagg atcactcaat gcacgatgcc
     6301 cgaaatctgt catatctgga ccgttatact cgtctgattt tatataattt tcaaaagccc
     6361 attcgttcca aatattatta ttatatttta ataagtattg gatgtttgta tctcctttaa
     6421 tgaaccataa taattcggtt gctactaatt taaaagaaac tttctttgtc gttaatagtg
     6481 gaaatccttt agataagtca aagcgaagtt gatgaccaaa tttcgaaatc gtacctgtat
     6541 ttgtgcgatc atttcgtgta tttcctattt ctaaaacttc ttcacaaaga ctgtgatatg
     6601 ctgcatcaaa tgaattcaac atatgcgata acacctcatt ttcattattt atagtatgta
     6661 tatttagttt gatataactt aattttatgt agcattttgt tatcactcat tttaggaata
     6721 tgatattaat atcatgaatt ccgttacttt atttataaaa tgctgattaa gtacctacca
     6781 catcgtaacg tgatatatgt ttccaat
//
LOCUS       DAMZLE010000118         6770 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMZLE010000118 DAMZLE010000000
VERSION     DAMZLE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.119"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            72..869
                     /locus_tag="POB92_002147"
     CDS             72..869
                     /locus_tag="POB92_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDI6579617.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1009..2769
                     /locus_tag="POB92_002148"
     CDS             1009..2769
                     /locus_tag="POB92_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDI6579618.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGIKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEVKGKIIATEIKEMV"
     gene            2770..3636
                     /locus_tag="POB92_002149"
     CDS             2770..3636
                     /locus_tag="POB92_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDI6579619.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3730..4023
                     /locus_tag="POB92_002150"
     CDS             3730..4023
                     /locus_tag="POB92_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDI6579620.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGMHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4157..5701
                     /gene="miaB"
                     /locus_tag="POB92_002151"
     CDS             4157..5701
                     /gene="miaB"
                     /locus_tag="POB92_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDI6579621.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            5702..6067
                     /locus_tag="POB92_002152"
     CDS             5702..6067
                     /locus_tag="POB92_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDI6579622.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6094..6585
                     /gene="thiW"
                     /locus_tag="POB92_002153"
     CDS             6094..6585
                     /gene="thiW"
                     /locus_tag="POB92_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDI6579623.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
BASE COUNT         2500 a          937 c         1287 g         2046 t
ORIGIN      
        1 agaatttaat atttacttta aatattagaa cgtatgaatt aaactagtat ttatgagagg
       61 atgaacaaaa catgagaata atgtttatag gggatatcgt aggtaaaatt ggacgagacg
      121 caattgaaac gtacatacct caactgaagc aaaagtataa accaacagtt acaattgtaa
      181 atgctgaaaa tgcagcacat ggtaaaggtt tgactgaaaa aatatataaa caattactaa
      241 gaaatggtgt agatttcatg actatgggta atcacacata tggtcaacgt gaaatttatg
      301 attttataga tgaagcaaaa cgtctagtaa gaccagcgaa ttttccggat gaagcgccgg
      361 gaattggtat gagatttata caaattaatg atattaaact tgcagttatt aatctgcaag
      421 gaagagcgtt tatgccagat attgatgatc cttttaaaaa ggcagatcaa ttagtcaagg
      481 aagcacaaga acaaactccg tttatatttg ttgattttca tgcagaaaca acttctgaaa
      541 agtatgcaat gggatggcat ttagatggta gagctagcgc tgttgttgga acgcatacac
      601 acattcaaac agcagatgaa cgtattttac caaaggggac agggtatata acggatgttg
      661 gtatgacagg tttttatgac ggcattttag gaataaataa aacagaggta attgagcgtt
      721 ttatcactag tttgccacaa agacatgttg ttccaaatga aggtagaagt gtattatctg
      781 gtgttgttat tgatttagac aaagaaggta aaacaaagca catcgaacgt atattgataa
      841 atgatgacca tccattttca acattttaaa attacgtaag taaacattcg aattggaccc
      901 tatcgtccat tagtatgaat ttaatatagt accactgttt acatagtaaa tcggtggttc
      961 tttttgttat catttaatat gaaatatatc cataggaggc atataactat gaaaccacaa
     1021 ttatcgtgga aagttggcgg tcaacaaggc gaaggtattg aatcaactgg ggaaatcttc
     1081 gctacggcta tgaatagaaa aggatattat ttatatggat atagacattt ttcaagtcgt
     1141 atcaaaggtg gacatacgaa taataaaatt agagtttcta cgacgcctgt tcatgcaatt
     1201 agtgatgatt tagatatttt gattgcattt gaccaagaaa caattgatgt taaccatcat
     1261 gaaatgagag aagacagtat tattttagct gatgccaagg ctaaacctgt gaaaccagaa
     1321 ggatgtcatg cacagcttat tgaattacct tttacagcaa ccgctaaaga attaggtaca
     1381 gcattaatga aaaacatggt tgcaataggt gctactagcg cattgatgaa tttgaataca
     1441 aatacatttg aagaacttat tactaatatg ttttctaaaa aaggtgacaa ggtagttgaa
     1501 gtcaatatcc aagcattaaa cgaaggttat caattaatgc aatcccgctt acctgaaatc
     1561 gacggggact ttgaattaga gtcaacagat gcactaccac atctatatat gattggtaac
     1621 gatgccattg gattaggtgc aattgctgca ggttcacaat ttatggcggc atatcctatt
     1681 acacctgcgt ctgaagttat ggaatatatg attgccaata tatctaaagt aaacggagcg
     1741 gttattcaaa cagaagatga aattgctgct gtaactatgg ctattggtgc aaattatggt
     1801 ggagtcagag cgtttacggc tagtgctggt ccaggtttat ctttaatgat ggaagcaatt
     1861 ggattatctg gtatgactga aacgccatta gtcattatta atactcaacg aggtggacct
     1921 tctactggat tacctacgaa acaagaacag tcagatttaa tgcaaatgat ttatggtaca
     1981 catggtgata ttccaaaaat agttgtagca ccaactgatg cagaagatgc attttattta
     2041 actatggaag cctttaattt agcagaacaa tatcaatgcc ctgttatagt tttaagtgat
     2101 ttgcaattat ctttaggtaa acaaactgtt gaaaaattag attataatcg cattgaaatt
     2161 aaacgtggtg aaatcattca atctgatatt gaacgtgaag aagatgataa aggttatttc
     2221 aagcgttatg cgttaacatc caatggtgtt tctcctagac ctatccccgg tattaaagga
     2281 ggtattcatc atataactgg tgtggaacac aatgaagaag gtaaacctag tgaatctgca
     2341 tcaaatagac aacaacaaat ggaaaaacga atgcgtaaaa ttgagcagtt actaattgaa
     2401 tcgccagtag aagctaactt acaacatgaa gatgcagata ttctttatat cggttttatt
     2461 tctacaaaag gtgcaattca agaaggtagt aaccgtttga atcaacaagg cataaaagtt
     2521 aacactatac aaattagaca attgcatcca ttcccaacaa gcgttattca agatgcagtt
     2581 aataaagcga agaaagtcgt tgtagtggag cacaattatc aaggacaatt ggctagtatt
     2641 ataaaaatga atgtcaatat tcatgataag attgaaaatt atacaaagta tgatgggaca
     2701 cctttcctac cacatgaaat cgaagtaaaa ggcaaaataa ttgctactga aataaaggag
     2761 atggtataga tggcgacatt taaagatttt agaaataatg ttaagcctaa ctggtgccct
     2821 ggatgtggcg atttctcagt acaagctgca attcaaaaag cagccgcaaa tatagggtta
     2881 gaacctgaag aagtagctat catcaccggt ataggatgtt ctggccgtct ttcaggatat
     2941 attaattctt atggcgttca ttctattcac ggacgtgcat tacctttagc tcaaggtgta
     3001 aaaatggcga ataaagattt aactgttatt gcatctggag gagatggtga tggttatgct
     3061 ataggtatgg ggcatacaat ccatgcttta agaagaaata tgaacatgac gtatatagtc
     3121 atggataatc aaatttatgg tttgacaaag ggacaaacat cgccgtcatc agcagtagga
     3181 tttgttacta aaacaacgcc aaaaggtaat atagaaaaaa atgttgcgcc tttagaatta
     3241 gcattatcat ctggtgccac atttgtagcc caaggttttt caagcgatat taaaggatta
     3301 acaaaactaa ttgaagatgc aattaatcat gatggatttt cattcgttaa tgtcttttca
     3361 ccatgtgtga cttataataa aattaacaca tacgattggt ttaaagaaca tttaacaagt
     3421 gttgatgaca ttgaaaatta tgattctaca gataaacaat tagcgactaa aactgttatt
     3481 gaacatgaat ctttagtaac tggtattgtt tatcaagata aagaaacacc atcatatgaa
     3541 tctcaaatta aagagttaga tgatacacca cttgctaaaa gagatatcaa aattactgaa
     3601 gacaccttca atgcattaac tgaacaattt atttaataat taaaactgag tcgtataaat
     3661 gtatttataa cagatccatt tatgctactc agttttttac tattacaaaa aataaaggag
     3721 tttttaaaaa tgaaagacac attaatgagt atacaaataa ttcctaaaac accaaacaat
     3781 gacaatgtta taccttacgt agacgaggcg attaaaataa ttgacgaatc tggtatgcat
     3841 tttagagtag gtccgttaga aacgacagta caaggaaata tgaatgaatg tttaatttta
     3901 atacaatcat taaatgaacg aatggtggaa cttgaatgtc caagtattat tagccaagtt
     3961 aagttttatc atgtgccaga tggcatcact attgaaactt taactgaaaa atatgatgaa
     4021 taacattaaa agtgaagtaa actggatttg aattggcttg ttagagatga cgtataactt
     4081 taactgtttt tgcactttat agttaaattt aatataatta ttaaatgata cgggcaaata
     4141 gaaaggattt tgtaaagtga acgaagaaca aagaaaagca agttctgtag atgttttagc
     4201 tgagagagat aagaaagcag aaaaagatta tagtaaatat tttgaacatg tttatcagcc
     4261 gcctaattta aaagaagcaa aaaaaagagg taaacaagaa gttcgttata atagagattt
     4321 tcaaattgat gaaaaatatc gcggtatggg gaacgagcgt acatttttaa ttaaaacata
     4381 tggatgtcaa atgaatgcac atgacactga ggtcattgct ggtatacttg aagcattagg
     4441 ctatcaagca acgactgata ttaacactgc agatgttatt ttaattaata catgtgcgat
     4501 tagagaaaat gccgagaaca aagtgtttag tgaaataggt aatttgaagc atttgaaaaa
     4561 agaacgacct gatattttaa tcggtgtttg tggttgtatg tcacaagaag agtcagtagt
     4621 gaataaaatt ttaaaatcgt atcaaaatgt agatatgata tttggtacac ataatattca
     4681 tcatttacca gaaattttag aagaagcata cttatctaaa gcaatggttg ttgaagtatg
     4741 gtctaaagaa ggagacgtta ttgaaaatct tccaaaagtc cgtgaaggca acattaaagc
     4801 atgggtcaat attatgtatg gttgtgataa gttttgtaca tattgcattg ttccatttac
     4861 aagaggtaaa gaacgaagcc gcagacctga agacattata gatgaagtac gtgaacttgc
     4921 tcgtgaaggt tacaaagaaa taacgctttt aggtcaaaat gtaaattctt atggtaaaga
     4981 tttacaggat atagaatatg acttaggaga tcttttacaa gcaatttcta aaatagcgat
     5041 tccaagagtt cgtttcacaa caagtcatcc ttgggacttt acagatcaca tgattgatgt
     5101 tatttcagag ggtggtaata tcgttcctca tatccacttg ccagttcaat ctggaaataa
     5161 tgcagtatta aaaataatgg gtagaaaata tacacgagaa agttatttgg atttagtaaa
     5221 acgaatcaaa gatagaattc ctaatgtagc attaactaca gatattattg tagggtatcc
     5281 aaatgaatca gaggaacaat ttgaagaaac tttaactctg tatgatgaag ttggttttga
     5341 acatgcatat acgtatttgt attcacaacg tgatggtacg cctgctgcta aaatgaaaga
     5401 taatgtacct ttaaatgtca aaaaggaacg attgcaacgt ttgaataaaa aagttggtca
     5461 ttattcacaa atagctatga gtaagtacga aggacaaact gtaacagtac tttgtgaagg
     5521 tagtagtaaa aaagatgatc aggttcttgc tggctacact gataaaaata agctagttaa
     5581 tttcaaagcg cctaaagaaa tgattggtaa actagtggaa gtacgaatag atgaagctaa
     5641 acagtattca ttaaatggca gttttgtaaa ggaagtagag ccggaaatgg tgattcaata
     5701 aatgtataat aaagatgacg tgttgaaaca agcggataat attgcaaata aaattaaaaa
     5761 tttggatact atcaaaacat atcaacaaat tgaagcacag attcatcaga accaaacgat
     5821 aaagactaaa atggatatgt taaaaaagca tcaaaaacaa gcagtaaact ttcaaaatta
     5881 cgggaaacaa aatgcgctag aacagtcgga acataccatt caaagtatag aagcagaaat
     5941 aaatacattg cccatagttg aacagtttca aacttcacaa tatgaagcga atcaattatt
     6001 gaaaatgttt gtatcaacaa tggaaacacg tttaaatgac cataataaag ccaagcatag
     6061 tgattaatta caaacaaaaa ggagaacaat catatgaaat caagaaaact ggctataact
     6121 gcacttttaa ttgcaataaa tgttgtatta agcagtatta tcatcattcc tctaggacca
     6181 gttaaggcag caccagtaca gcattttgta aatgtattaa gtgcggtcat agtaggtcct
     6241 tggtatggat tagctcaagc gcttatatca tcaattttaa gagttctttt tggtactggt
     6301 acagcttttg catttccagg tagtatgatt ggagttttat tggctagtat gttttacata
     6361 tatcgtaaac atatattcat ggccgcggtc ggtgaagtac ttggaactgg tgtcatcgga
     6421 agtttaattt gtataccatt agcatatttc cttgggcttc aagacttctt cattaaaccg
     6481 ttaatgatta cgttcatagt ctcaagtgct atcggatcta ttataagtta tttcttatta
     6541 attactctaa aaaaacgtgg tattcttcaa aggtttataa aataatttta tgatatatga
     6601 tttcaaacag agtcattgtt cattttaata ttgaacgccc ttacttaatt aaatgtcttg
     6661 aatgttagaa ttttaattta attctaaaca tgctattgac acattttatt aggtttgagg
     6721 cgttttctta tacatttaag atgcttcgtg attatatggt atgaataggt
//
LOCUS       DAMZLE010000119         6727 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMZLE010000119 DAMZLE010000000
VERSION     DAMZLE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.120"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            208..609
                     /locus_tag="POB92_002154"
     CDS             208..609
                     /locus_tag="POB92_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6579624.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(729..1292)
                     /locus_tag="POB92_002155"
     CDS             complement(729..1292)
                     /locus_tag="POB92_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6579625.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1289..2242)
                     /locus_tag="POB92_002156"
     CDS             complement(1289..2242)
                     /locus_tag="POB92_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI6579626.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2352..3533
                     /locus_tag="POB92_002157"
     CDS             2352..3533
                     /locus_tag="POB92_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579627.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTQLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3821..6244
                     /locus_tag="POB92_002158"
     CDS             3821..6244
                     /locus_tag="POB92_002158"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI6579628.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEAEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDE
                     YKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYG
                     TGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEA
                     AITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPL
                     LLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYID
                     PKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKL
                     FNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLD
                     GSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQ
                     LMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEA
                     LLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVP
                     QKLVNIVAK"
     gene            6266..6577
                     /locus_tag="POB92_002159"
     CDS             6266..6577
                     /locus_tag="POB92_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6579629.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2252 a          960 c         1292 g         2223 t
ORIGIN      
        1 tcttatttct aaatattaac tctattgatt attggttttt atacttattt aattttattc
       61 aactttgaca attgaataga aagcaagttt atttacactt gtagttttat gcataagtta
      121 gcacatacaa gtttcgggat tgttgggatg tttgttaata cttgtatggt agctaaatat
      181 gtgattatta attgggagat gtttagcatg aaaaaagtaa ataacgacac tgtatttggg
      241 attttgcaat tagaaacact tttgggtgac attaactcaa ttttcagcga gattgaaagc
      301 gaatacaaaa tgtctagaga agaaatttta attttactaa ctttatggca aaaaggttct
      361 atgacgctta aagaaatgga cagatttgtt gaagttaaac cgtataagcg tacgagaacg
      421 tataataatt tagttgaatt agaatggatt tacaaagagc gtcctgttga cgatgaaaga
      481 acagttatta ttcattttaa tgaaaagtta caacaagaga aagtagagtt gttgaatttc
      541 atcagtgatg cgattgcaag tagagcaaca gcaatgcaaa atagtttaaa cgcaattatt
      601 gctgtgtaag tttaatagca taaaaagagg ttttcattaa gttgaaaacc tctttttgtt
      661 gttggtatta atttttcaaa tgttgactac tcaatcctaa attataaata gtatagcgca
      721 gcaaatgctt aagaaatttt ttctatggca caaatgaatg gagcatgatt acgttggttt
      781 aaaaattgat attgcaaaac ttgcgcatgc ttttgatcta aagtactcaa gtaatcaagc
      841 aatgcatgct tctcaatttg tccttcgcta tgaccatgat atataacaag tacaataata
      901 ccttcaattg acattaatga tagcaatgaa ttaatagctt ggattgtcgt gtcaggcttt
      961 gtcacgatag atttatcacc tttaggcaaa taacctaggt taaagatggc tgcatcaata
     1021 tgacctttat gtgcatcatt tatatgatgt tcaatatttt catgtccatc ttttattaaa
     1081 gaaacatgat tgaaatcctt aactttatca cgtgtatttt ccaaagctaa atcttgaatg
     1141 tcgaaaccat aaacatgtcc ttctggtact tgttcggcta aaaataaagt gtcattgccg
     1201 ttaccgcaag ttgcgtctac aacaatactt tctggtgtta tatgttgttt aataagtgtt
     1261 tttgaaaaag ggagtatacg ttctaatttc attgcttcac cttagacttg taacgcaagc
     1321 cttgatatga atttctacgt gctaattcag catcgatgcc atttaatact tcccatttat
     1381 taacactcca cattggaccg accatgatat ctattggacc atcaccggta attcggtgaa
     1441 cgatcatttc agggggaatc acttctaatt ggtccacaac taggtttgtg tactcttctt
     1501 gagtcataaa agttaataaa cctttatcgt attgttttac catcggtgta ccttttaaca
     1561 aatgaagtaa atgaatttta ataccttgta catccatttg tgcaacttct ttggcagtag
     1621 ccatcatcat gtcatagtct tcgccaggta agccattaat gatgtgtgta catacattga
     1681 tattatgctt acgtaatttt gccacaccat cataataagt tttcatatca tgggcacgat
     1741 tgattaaatc agatgttgat tgatggattg tttgtagtcc taattcaacc cataagtatg
     1801 ttcgttgatt caaatctgct aaatattcga caacatcgtc tggtagacag tcaggacgcg
     1861 taccaataga taatcctaca acacccggtt ctttaagtac aggttcgaat ttttctttta
     1921 atacttcaac tggcgcatgt gtatttgtaa atgcctgaaa ataagcaata tattttcctt
     1981 cgtgccattt ctcatgcatc ttttctttaa tttctttaaa ttgtactgcg attgaatctg
     2041 cacgattacc tgcaaagtct ccgctacctg cagcagaaca aaatgtacat ccaccatgtg
     2101 ctacagtgcc atcgcggtta ggacagtcaa acccgccatc caatgcaact ttaaatattt
     2161 tttgtccaaa tttatttttt aaatggtaat tccatgtgtg ataacgtttg ttttcaaaag
     2221 cgtattggaa atgattgccc atatgtcatt ttcctttcta taaaaaaaga gttctaagta
     2281 cagattttaa catattttaa tgttatagtg tttattatag tttgacaaaa aagagagagg
     2341 aactatgaaa tatgaatata cctaaatcag tctggtggct agtaattggc atggcgttaa
     2401 atattactgg ttccagtttt ttgtggcctt taaatacaat ttatatgaaa caagaacttg
     2461 gaaaaagttt aactgttgct ggtttagtgc taatgataaa ttcatttggc atggttattg
     2521 gaaacttatt aggtggttca ctatttgata aattaggtgg atacaagacg attttaattg
     2581 gaactttcac ttgtctttgt agtacaacgc tacttaattt ctttcacggg tggccttggt
     2641 atgctgtatg gcttgtaatg ttagggtttg gtggcggaat gattattcct gcgatatacg
     2701 ctatggctgg agcagtgtgg ccaaatggcg gaagacaaac gtttaatgcg atatacttag
     2761 cgcaaaatat tggtgtggct gtcggtgctg caatgggcgg ctttgtcgca gaatttagct
     2821 ttaactatat ctttttagcc aatcttatta tgtatgttgt gtttgcgctt gtcgcggtaa
     2881 cgcaatttaa tattgaaatt aatgcgaaag ttaaatatcc aactcaatta gatattactg
     2941 gtaaaaagaa taaagcaaga tttatttcat tagtactaat ttgtgcaatg tttgcaattt
     3001 gttgggttgc atatattcaa tgggagtcta caatcgcttc atttacacaa tctattaata
     3061 tttcaatggc acaatatagt gttttatgga caattaacgg aataatgatt ttagtagcac
     3121 aaccattaat taaaccgatt ctctatctgt taaaaggaaa cttaaagaag caaatgtttg
     3181 tcggcatcat catttttatg ttgtcgttct ttgtcacgag ttttgccgaa aactttacaa
     3241 tatttgttgt cggtatgatt attttaactt ttggagaaat gtttgtatgg ccagcagttc
     3301 caactatagc caatcagtta gcgccagatg gtaagcaagg acagtaccaa ggttttgtga
     3361 attcagctgc tacagtagga aaagcatttg gtccatttct tggtggtgta ttagttgatg
     3421 cgtttaatat gcgcatgatg tttatcggta tgatgctact acttgtattt gcattaatat
     3481 tattaatggt tttcaaggag aataatacgc aacctaaaaa aatagatgca taatgagtaa
     3541 atagaattaa cgttatagac ttgaaataaa tgtcgttata acataatatt aatttgtata
     3601 atttaatttc gtttggagct tttctacaga aagctagtga tgctgagagc tagtgttaag
     3661 gactaaatgt aaatcgtatt aattttaaat tgaatgaatg acatctctta ctattaaaat
     3721 gagtgcacaa tttttgtgaa atagggtggt aacgcggcaa atgtcgtccc tatgtaaata
     3781 gaatagttag aggtgtcttt tttattgaat aggaggaaat gtgttgaatt acaaccacaa
     3841 tcaaattgaa aagaaatggc aagactattg ggacgaaaat aaaacattta aaacaaatga
     3901 taacttaggt caaaagaaat tttatgcttt agacatgttt ccatatccat caggtgctgg
     3961 tttacatgtt ggacatcctg agggctatac agcaacagat atcatttcaa gatataaaag
     4021 aatgcaagga tataatgtat tacatccgat ggggtgggat gcattcggat taccagcaga
     4081 gcaatatgct ttagacactg gcaacgaccc acgtgaattt acaaagaaaa atatccaaac
     4141 ttttaaacga caaattaaag aattagggtt cagttatgat tgggatcgtg aagttaatac
     4201 aacagatcca gaatactata aatggacaca gtggattttc atacagttat ataacaaagg
     4261 tttagcatac gttgatgaag ttgcagttaa ctggtgtcca gcattaggca ctgttttatc
     4321 taacgaagaa gtgattgatg gtgtctctga acgtggtgga catccagttt atcgtaagcc
     4381 gatgaaacaa tgggtactta aaatcacaga atatgcagat caattattag cagatttaga
     4441 tgatttagat tggcctgaat ctttaaaaga tatgcagcgc aattggattg gacgttctga
     4501 aggggccaaa gtttcatttg atgtagataa tacggaagcg gaaggaaaag tagaagtatt
     4561 tacgactaga ccagatacaa tctatggtgc atcattctta gtcttaagtc ctgaacatgc
     4621 attagttaat tcaattacaa ctgatgaata taaagaaaaa gtaaaagctt atcaaacaga
     4681 agcttctaaa aagtcagatt tagaacgtac agatttagca aaagataaat caggtgtgtt
     4741 tactggtgca tatgcaatta atcctttatc tggtgaaaaa gtacaaattt ggattgctga
     4801 ttatgtatta tcaacatatg gtactggagc aattatggca gtaccagcgc atgatgacag
     4861 agattatgaa tttgctaaaa agtttgattt gccaatcatt gaagtcatcg aaggtggaaa
     4921 tgttgaagaa gcagcataca ctggtgaagg taaacatatt aattctggtg aacttgatgg
     4981 tttagaaaat gaagcggcaa ttactaaagc tattcaatta ttagagcaaa aaggtgctgg
     5041 cgaaaagaaa gttaattaca aattaagaga ttggttattc agtcgtcagc gttattgggg
     5101 cgaaccaatt cctgtcattc attgggaaga tggaacaatg acaactgttc ctgaagaaga
     5161 gctaccattg ttgttacctg aaacagatga aatcaagcca tcagggactg gtgagtctcc
     5221 actagctaat attgattcat ttgtaaatgt tgtagatgaa aaaacaggta tgaaaggacg
     5281 tcgtgaaaca aatacaatgc cacaatgggc aggtagttgt tggtattatt tacgttacat
     5341 cgatcctaaa aatgaaaata tgttagcaga tcctgaaaaa ttaaaacatt ggttacctgt
     5401 tgatttatat atcggtggag tagaacatgc ggttcttcac ttattatatg caagattttg
     5461 gcataaagtc ctttatgatt tgggtatcgt acctactaaa gaacctttcc aaaaattatt
     5521 taaccaaggt atgattttag gagaaggtaa tgagaagatg agtaaatcta aaggaaatgt
     5581 aatcaatcct gatgatatag tacagtctca tggtgcagat actttacgtc tttacgaaat
     5641 gtttatggga cctttagatg ctgcaattgc atggagtgaa aaaggattag atgggtctcg
     5701 tcgattctta gatcgcgtat ggcgtttaat ggtaaatgaa gatggaacat tgagttcaaa
     5761 aattgtaact acaaataata aatctttaga taaagtttat aaccaaactg ttaaaaaggt
     5821 aacagaagac tttgaaacat taggatttaa tactgctatt agtcaattaa tggtatttat
     5881 taatgagtgt tataaagttg atgaagttta taaaccttac attgaaggct tcgttaaaat
     5941 gttagcacct attgcaccac atatcggtga agaattatgg tcaaaattag gacatgaaga
     6001 gtctattacg taccaacctt ggccaactta tgacgaagca ctacttgtag atgatgaagt
     6061 agaaatcgtt gttcaagtaa atggtaaatt gagagctaaa attaaaattg ctaaagatac
     6121 atcaaaagaa gaaatgcaag aaattgcctt atctaatgac aatgttaaag cgagtattga
     6181 aggtaaagac atcatgaaag tcatcgctgt tcctcaaaaa ttagtcaata ttgtagctaa
     6241 ataatgtttt aaggaggact ttgaaatgaa gtcaattact acagatgaat taaaaaacaa
     6301 acttttagaa tctaaaccag ttcaaattgt tgatgttcgt actgacgaag aaacagcaat
     6361 gggatatatt cctaatgcaa agttgattcc aatggatacc attccggata atttaaattc
     6421 atttaataaa aatgaaatat attatattgt atgtgctggt ggagttcgaa gcgctaaagt
     6481 tgtagaatat ttagaggcaa atggcattga tgccgtaaat gtcgaaggcg gcatgcacgc
     6541 atggggcgat gaaggtttgg aaataaaaag tatttaaagt agtgacataa tttaaaataa
     6601 tattacattt gtaatgacac caagtaacgt ttcggttgct tggtgttttt tggtatgaat
     6661 tactttctgt tacaaaacaa tctaaagcgt tcttgttatg ttttattaag attttaatta
     6721 caaaacg
//
LOCUS       DAMZLE010000120         6687 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMZLE010000120 DAMZLE010000000
VERSION     DAMZLE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.121"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..408
                     /locus_tag="POB92_002160"
     CDS             <1..408
                     /locus_tag="POB92_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDI6579630.1"
                     /translation="DMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRV
                     KNRVELNTPKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFL
                     AILEMSKAGIINIEQQRNFEDINIIRGVNYHFG"
     gene            401..943
                     /gene="scpB"
                     /locus_tag="POB92_002161"
     CDS             401..943
                     /gene="scpB"
                     /locus_tag="POB92_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDI6579631.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKNQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            936..1673
                     /locus_tag="POB92_002162"
     CDS             936..1673
                     /locus_tag="POB92_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI6579632.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            1806..2531
                     /gene="srrA"
                     /locus_tag="POB92_002163"
     CDS             1806..2531
                     /gene="srrA"
                     /locus_tag="POB92_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDI6579633.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            2512..4263
                     /gene="srrB"
                     /locus_tag="POB92_002164"
     CDS             2512..4263
                     /gene="srrB"
                     /locus_tag="POB92_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDI6579634.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            4508..5416
                     /locus_tag="POB92_002165"
     CDS             4508..5416
                     /locus_tag="POB92_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDI6579635.1"
                     /translation="MKKFIGSVLTTTLILGGCSAMENESNKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDK
                     EFKEGMQQARKELDYTANTDAVSTLFSTKKNFTKDNTVDDVIELSNKLKEKPNMPQKS
                     QVTIQLGKSSINTKKPFYDDINPIEG"
     gene            5428..>6687
                     /locus_tag="POB92_002166"
     CDS             5428..>6687
                     /locus_tag="POB92_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579636.1"
                     /translation="MNKINDRDIAELSSYWVYQDINEKKDFKVNGKRFKQIDNYNDNS
                     NDNMNGAADIKTYELLNDQGNPTGQQTIIYQGTSNEAINPNNPLKSSGFGDDWLQNAK
                     LMNNDNESTDYLKQVEEFANSYRQKIKDASVLDENEFRRKYKTKPYNYKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDSAMIPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNISDGVPRLDALIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGKKVKKTINITLDMDGRIPINVWTGDSIARSGKGTLIKLNLENLDALSKLITGETS
                     GMLAECVIFLNESFNISENENKNFADRKKQLSEGFKDKINLFQLEEMERTLISKINAL
                     EEVADETIESISAVKHLLPDFALDALKE"
BASE COUNT         2606 a          866 c         1260 g         1955 t
ORIGIN      
        1 gacatgaaag aagaaagaga tttttatttt actaaaagac cgacagattt atctcatttg
       61 gaaacggatg aatcttggga tccaaatcat acgattgatt taactgaatt aattgtagct
      121 tatcaaagag ttaaaaatag agttgagtta aatacaccta aatctgttga aatccgaaaa
      181 gagacattta ccattcaaca agctacagaa caagtgacat cgagattgaa agataaagat
      241 cattttaact tctttagtct gtttacgttt tctgagccaa ttgaacaagt agtcactcac
      301 tttttagcta ttttagagat gtcaaaagca ggaataatta atattgagca acaacgtaat
      361 tttgaagata ttaacattat tagaggagtg aactaccatt ttggataatc atggtatatt
      421 agagtcgcta ttatttacag ctggcgatga aggtttagat gaaaaacaac tattagaaat
      481 attagatatg tcgaaaaatc aactcgttga attaattgaa aattattcat cacatggatt
      541 aatgatacaa cgatttggaa cgacgtatgt tttaacgact aaaaaagaag cggcaacgta
      601 tattgaacaa ttaattgaac aaaagtcaca aatgaaatta tcacaagcag caatggaagt
      661 tctatcaatt attgcttata accagccatt atcaagaagt gatattgaat taattcgtag
      721 tatcaattca gatggtgcgg tcaagacatt gattgccaaa ggactagttg aggctaaagt
      781 ggtcaatgaa cagcgtagcc aacagttaat tactactgat ttatttttaa atgtatttgg
      841 tatttccaat atagaagatt tgccgacaac tgaagaagac gatgaagaaa tggatgcttt
      901 cttcagtaat ctagtcaatc aaaaaggaga aaataatgac taaagaatta gaacgattac
      961 aaaaacgtat agctaatagc ggttatactt caagacgtaa ggcagaaact ttaatttctg
     1021 aaggaaaagt gaaagtgaat ggtacgactg tcactgaatt aggaacaaaa gtaaagccat
     1081 cagatacgat tgaagttgaa ggtattaaaa tagaactaga agacaaaatt tacattttat
     1141 tccataaacc aactcaagtg ataacaagtg tatcagatga tagaggacgt acagtggtta
     1201 cggattactt taaagatata gaagcacgta tttatcctgt tggtcgttta gactatgata
     1261 cttctggatt gttattactt acaaatgatg gtgaatttac taatttaatg acacatccaa
     1321 gatatcaaat taagaagaaa tatgtagcaa aattaaaagg ttacttaatg agagaagaag
     1381 tgaaagccct agaaaaaggt attgaattag aagatggtat gacgcaaccg gctgaagtga
     1441 aagttaagaa acaagataaa gataaaaaca caacattggt tgaaattaca attacagaag
     1501 gtcgtaatcg ccaagtcaga agaatgttcg aacattttgg tcatcaagtg acgaaactgt
     1561 ctcgtattga atatgggcct ttaaatgttg tcggtttgaa tgctggtgaa ggacgtgtat
     1621 tgacgcctca tgaagtaaaa gtgatgcgtc atttagcaga acatgggaaa taattaaata
     1681 aaatatgtat ttttcacaaa gtttgagaat tttttcacaa aattttaggt ttttaataac
     1741 tgtatgcgct ttcctgtgat ataatacttt tgtaaagaaa agcatgtgtg ggaggtatga
     1801 cctgtatgtc gaacgaaata cttatcgtag atgatgagga tagaatcaga agattactta
     1861 aaatgtattt agaaagagaa tcttttgaaa tccatgaagc aagtaatggc caagaggctt
     1921 atgaacttgc aatggagaat aattatgctt gcatactact agatttaatg ttgcctgaaa
     1981 tggatggtat ccaggtggca actaaattgc gtgaacataa acaaacaccg attattatgt
     2041 tgactgctaa aggtgaagaa acaaaccgtg ttgaaggttt tgaatctggt gcagatgatt
     2101 atatcgtcaa accattttca ccaagagaag tagtcttaag agttaaagca cttctaagaa
     2161 gaacgcaatc tacaactgta gaacaaagcg aacctcacgc acgtgatgtg attgaattta
     2221 aacatttaga aatagataat gatgcacatc gcgtacttgc tgataatcaa gaagttaatt
     2281 tgactcctaa agagtacgaa ttattaatat atttagctaa aacaccaaat aaagtatttg
     2341 accgtgaaca attattaaaa gaagtttggc attatgaatt ctatggtgat ttaagaacag
     2401 ttgatactca tgttaaacga cttagagaaa agttaaatcg tgtgtctagt gaagctgcgc
     2461 atatgattca aacagtctgg ggcgttgggt ataaatttga ggttaaatct aatgatgagc
     2521 cggctaaata gtgtcgtaat taaactgtgg ttaactatta ttttaatagt gacgacagtt
     2581 ttaattttat taagtattgc tttaattacc tttatgcaat actatttcac acaagaaacc
     2641 gaaaatgcca taagagaaga tgctagacgt ataagttcac tggtcgaaca atcacataat
     2701 aaagaagaag caataaaata tagtcaaaca ttaattgaaa atcctggtgg gttgatgatt
     2761 ataaataata aacatcgtca atcaacggct tcactttcta atattaaaaa gcaaatgttg
     2821 aatgaagtag tcaacaacga ccattttgac gatgtgtttg ataaaggtaa atctgttact
     2881 cgaaatgtaa cgattaaaga aaagggctca tctcaaacat atattttgtt aggctatcca
     2941 acaaaagcac agaagaatag tcatagcaaa tatagtggag tctttatata taaagacttg
     3001 aaatcaatcg aagatacaaa taatgctatt acgattatca ctataattac ggctgttatt
     3061 ttcttaacaa ttacaacagt ctttgcgttt ttcttatcat caagaattac aaaaccttta
     3121 agacgtttaa gagaccaagc tacacgtgta tctgaagggg attactctta taaaccttct
     3181 gtcacaacga aagatgaaat tggtcaatta tcgcaggcat ttaatcagat gagtacagaa
     3241 atcgaagagc atgtcgacgc attatccaca tctaaaaata ttagagacag cttaattaac
     3301 tctatggtag aaggtgtcct aggtattaat gagagtcgac aaattatctt atctaataag
     3361 atggcgaatg atattatgga caatattgat gaagatgcta aagctttctt attaagacaa
     3421 attgaagata cttttaaatc aaaacaaacg gaaatgcgtg atttagaaat gaatgcacga
     3481 ttctttgttg tgaccacaag ctatatcgac aagattgaac agggaggtaa aagtggtgtt
     3541 gttgtgacag ttcgtgatat gactaatgag cacaatctag atcaaatgaa gaaagatttc
     3601 attgctaatg tatcacatga attacgtaca ccgatatcat tacttcaagg ttatactgaa
     3661 tcaattgtag atggtattgt tacagaaccg gatgaaataa aagaatcgct tgccattgtc
     3721 cttgatgaat cgaaacgttt aaatcgttta gttaatgaat tgttaaatgt cgcacgcatg
     3781 gatgctgaag ggttatccgt aaataaagaa gttcagccta ttgcagcgtt actagataag
     3841 atgaaaatta agtatcgcca acaagctgat gatttaggtc taaatatgac ttttaattat
     3901 tgtaagaagc gtgtttggag ttatgatatg gatcgcatgg accaagtact aacgaactta
     3961 attgataatg catcacgtta tacgaaacct ggagatgaaa ttgcaattac ttgtgatgaa
     4021 aatgaaagcg aagatatttt atacattaaa gatacaggta caggcattgc accagaacat
     4081 ttacaacaag tatttgatcg tttttataaa gttgatgcag cgagaacgcg aggtaaacaa
     4141 ggtaccggtt taggtttgtt catttgtaaa atgattatcg aagagcatgg tggttccata
     4201 gatgttaaaa gcgaattagg gaaaggcaca acatttatta ttaaactacc aaaaccagaa
     4261 taaaattgaa tatagttatt tcagaacgca tgttattgat tagagactct aatttatagc
     4321 atgcgttttt tgattgatgt ggggaatttt gtatgtggat tagaacttag ggtttttgcg
     4381 aatatcaact attaaatata ttactaattt atacaaaaat ataaagtttg ataaagttat
     4441 ttatttgatt ataaaaatag ggtaaaatat agatatattg tattaattaa attattcgag
     4501 gtgtcatatg aaaaagttca ttggatcagt tttaaccacg acattaattt tagggggatg
     4561 ttccgcgatg gaaaatgaat caaataaaga cacaaacaca gaaacaaaat ctgtaccaga
     4621 agaaatggaa gcttcaaaat atgtaggcca aggcttccaa ccgcctgcag aaaaagatgc
     4681 gattgaattt gcgaagaagc atcgtaaaga atttgaaaaa gtaggtgaac aattctttaa
     4741 agataacttt ggactaaaag ttaaagctac aaatgttgta ggtaaagatg atggtgtaga
     4801 agtttatgtg cattgtgaag atcatggcat tgtatttaat gcaagtctac ctttgtacaa
     4861 agatgccatc catcaaaaag gatcaatgcg cagtaatgac aatggtgatg atatgagtat
     4921 gatggtgggt acagtgctga gtggctttga atatcgagcg caaaaagaaa agtatgataa
     4981 cttatataaa ttcttcaaag aaaatgaaaa gaaatatcaa tatacaggct ttacaaaaga
     5041 ggcaattaac aagacacaaa acgttggata tcaaaatgaa tacttttata tcacatattt
     5101 atcaagaaac ttaaaagaat atcgtaaata ttacgaaccg ttgattcata aaaatgataa
     5161 agaatttaaa gaaggtatgc aacaagctag aaaagagtta gattatactg ccaatactga
     5221 tgcagtttct acactttttt caactaagaa aaactttact aaagacaata cagtagatga
     5281 tgtgattgaa ttgagtaata aattaaaaga aaaacccaat atgcctcaaa aatctcaagt
     5341 tactattcaa ttaggaaaat ctagtataaa tactaaaaaa ccattttatg acgatataaa
     5401 tccaattgaa gggtaagtga atataaagtg aataaaataa atgataggga catagcagaa
     5461 ttgagcagtt attgggttta tcaagatata aatgaaaaaa aagattttaa agtaaatgga
     5521 aaacggttca aacaaataga taattataat gataatagta atgataatat gaatggtgct
     5581 gctgatatta aaacatacga gttacttaat gatcagggga atccaactgg tcaacagaca
     5641 ataatttatc aaggaacatc taatgaggca attaacccaa ataatccatt aaaatcatcg
     5701 ggttttggag atgattggct ccaaaatgct aaattaatga ataatgataa tgaaagcaca
     5761 gattatttaa agcaagtaga agaattcgca aatagttatc gacaaaaaat taaagatgct
     5821 tctgttcttg atgaaaatga atttagaaga aaatacaaaa ctaaacctta taattacaaa
     5881 aacaaaacaa ttgtggcgga tggtggtaat tcggaaggtg gtgcaggagc aaaatatcaa
     5941 ggagcgaaac atccgaatga aaaagttgtt gctactgact cagcaatgat tccttatgct
     6001 gcttggcaga aatttgctag accacgcttt gataatatga ttagttttaa tagtaccaac
     6061 gatttattaa catggttaca agatccattc atcaaagata tgccaggaaa acgcgttaac
     6121 attagtgatg gtgtgcccag gttagatgct ttaatagaca gccatgtagg ttataaaagg
     6181 aagttaaata gaaaagataa cacatacgat actgtaccac taatcaaaat taagtcggta
     6241 aaagatacag aaattaaaaa tggaaaaaaa gtaaaaaaga ctattaacat aacattagat
     6301 atggatgggc gaattccaat aaatgtttgg acaggagatt cgattgcacg ttccggaaaa
     6361 ggaactttaa ttaaacttaa tttagaaaat cttgatgcgt tgagtaaact gattactggt
     6421 gaaacaagtg gtatgttagc agaatgcgta atctttttaa atgaaagttt taacatctca
     6481 gaaaatgaaa ataaaaattt tgcagataga aagaaacaat tatcagaagg atttaaggat
     6541 aagattaact tatttcaatt agaagaaatg gaaagaactt taattagtaa aataaacgca
     6601 cttgaagaag ttgcagatga aacaatagaa agtattagtg ctgttaaaca cttattacct
     6661 gattttgcat tggatgcatt aaaagaa
//
LOCUS       DAMZLE010000121         6464 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMZLE010000121 DAMZLE010000000
VERSION     DAMZLE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.122"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            120..1349
                     /locus_tag="POB92_002167"
     CDS             120..1349
                     /locus_tag="POB92_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDI6579637.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1544..2401
                     /locus_tag="POB92_002168"
     CDS             1544..2401
                     /locus_tag="POB92_002168"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDI6579638.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNNMRQTLSEI
                     LKIHQEVTK"
     gene            2401..3345
                     /locus_tag="POB92_002169"
     CDS             2401..3345
                     /locus_tag="POB92_002169"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDI6579639.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3614..4582
                     /gene="pfkA"
                     /locus_tag="POB92_002170"
     CDS             3614..4582
                     /gene="pfkA"
                     /locus_tag="POB92_002170"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI6579640.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4604..6361
                     /gene="pyk"
                     /locus_tag="POB92_002171"
     CDS             4604..6361
                     /gene="pyk"
                     /locus_tag="POB92_002171"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI6579641.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2287 a          873 c         1330 g         1974 t
ORIGIN      
        1 ataatcaaaa ccataaattc ttactcattc agatttaccc aatattttta cttttataat
       61 gtaatgcgtt ttatccaagt tattttttaa aaataaatat tgaattaggg gctgatttca
      121 tgtcattaag agatgaagca ttggaaatgc acaaacgtaa tcaaggtaaa ttagaagtta
      181 aaccaaatgt aaaagttact aataaagagg aattaagttt agcatactca cctggcgttg
      241 ctgaaccgtg taaagatatt tatgaagata aaagaaaagt atatgattac acaattaaag
      301 gaaatacagt tgcagttatt actgatggaa cagcggtatt aggtttaggt aacattggac
      361 ctgaagcaag tattcctgta atggaaggta aagcagtatt attcaaaagc ttcgctggta
      421 tcaatggggt gcctattgcg ttaaatacaa ctgataccga agaaatcatt aaaacagtta
      481 agttgttaga acctaattat ggtggtatta atttagagga tatttcggca ccacgttgtt
      541 ttgaaattga agaacgattg aaaaaagaaa ctaatattcc ggtattccat gacgatcaac
      601 atggtacagc aattgtaaca atggcaggtt tggtaaatgc attgagagtt gttaacaaag
      661 atattgctaa aataaaagtt gtactaaatg gtgctggtgc agcaggaata gccattgtta
      721 aattactata cgcgtatggt gtaagaaata tggttatgtg tgactcaaga ggcgcaattt
      781 ttgaaggacg ttcatatggt atgaatccta cgaaagatgt tgtagctaaa tggacaaata
      841 aagataagat tgaagggtct ttagaagaag tcgtaaaaga cgcagatgta tttatcggag
      901 tttctgtagc taatgcgctg tcacaagata tggttaagag tatggcagat aatccaatta
      961 tatttgcaat ggctaatcca aatcctgaaa taatacctga tgatgccaaa gcggcaggtg
     1021 cacgagttgt tggtacagga cgttcagact atcctaacca aattaataat gtattagctt
     1081 tccctggtat ttttagaggt gcattagagg ttgaagctac acatataaat gaagaaatga
     1141 aaaaggcagc tgtagaagcg attgctgatt taatcgatag ttctgaatta aatgaagact
     1201 actgtatccc aggaccgttt gataaacgtg tagcgccatc agttgctcgt aatgttgcta
     1261 aagcggcaat ggaatctgga gtagctagga ttgaagttga tccgcaagat gtgtatgata
     1321 aaacaatgaa acttacagat ttacaataaa aataattaac gtttaattaa attgcttaaa
     1381 agttagcaat aaagagtcat taaaatgatg aaaatgaaag tttatgataa acattcaaca
     1441 gtcaaacgaa tataaatcaa ataaatttaa acccgttttt aactggtcaa gttcagttta
     1501 aggctctaaa tggttagaac agaggttatt tggaggtttt cctatgttta aagatttttt
     1561 taatcgaaca aagaaaaaga aatatcttac agtacaagac tctaaaaata atgatgtgcc
     1621 tgcaggtatt atgactaagt gtccaaagtg taagaaaatt atgtacacaa aagaattagc
     1681 tgaaaattta aatgtgtgct ttaattgtga tcatcatatt gctttaactg cgtataaacg
     1741 tatagaagca atttctgatg aaggatcatt tacagaattc gataagggaa tgacctctgc
     1801 gaatccatta gattttccaa gttatttaga aaaaattgaa aaggaccaac aaaagacagg
     1861 tcttaaagaa gcagttgtga ctggtacagc gcaactagat ggtatgaaat ttggcgttgc
     1921 tgtcatggat tcacgtttta gaatgggaag tatgggatcg gttatcggtg aaaagatatg
     1981 tcgcatcatt gattactgca ctgagaaccg tttaccattt attcttttct ctgcaagtgg
     2041 tggtgcacgt atgcaagaag gtattatttc cttgatgcaa atgggtaaaa ccagtgtatc
     2101 tttaaaacgt cattctgacg ctggactatt atatatatca tatttaacac atccaactac
     2161 tggtggtgta tctgcaagtt ttgcatcagt tggtgatata aatttaagtg agccaaaagc
     2221 gttgataggt tttgcaggtc gtcgagttat tgaacagaca ataaacgaaa aattgccaga
     2281 tgatttccaa actgcagaat ttttattaga gcatggacaa ttggataaag ttgtacatcg
     2341 taataatatg cgtcaaacat tgtctgaaat tctaaaaatc catcaagagg tgactaaata
     2401 atgttagatt ttgaaaaacc actttttgaa attcgaaata aaattgaatc tttaaaagaa
     2461 tctcaagata aaaatgatgt ggatttacaa gaagaaattg acatgcttga agcgtcattg
     2521 gaacgagaaa ctaaaaaaat atatacaaat ctaaaaccat gggatcgtgt gcaaattgcg
     2581 cgtttgcaag aaagacctac gaccctagat tatattccat atatctttga ttcgtttatg
     2641 gaactacatg gtgatcgtaa ttttagagat gatccagcaa tgattggtgg tattggcttt
     2701 ttaaatggtc gtgctgttac agttattgga caacaacgtg gaaaagatac aaaagataat
     2761 atttatcgaa attttggtat ggcgcatcca gaaggttatc gaaaagcatt acgtttaatg
     2821 aaacaagctg aaaaattcaa tcgtcctatc tttacattta tagatacaaa aggtgcatat
     2881 cctggtaaag ctgcggaaga acgtggacaa agtgaatcta tcgcaacaaa tttgattgag
     2941 atggcttcac taaaagtacc agttattgcg attgtcattg gtgaaggtgg cagtggaggt
     3001 gctctaggta ttggtattgc caataaagta ttgatgttag agaatagtac ttactctgtt
     3061 atatctcctg aaggtgcagc ggcattatta tggaaagaca gtaatttggc taaaattgca
     3121 gctgaaacaa tgaaaattac tgcccatgat attaagcaat taggtattat agatgatgtc
     3181 atttctgaac cacttggcgg tgcacataaa gatattgaac agcaagcttt agctattaaa
     3241 tcagcgtttg ttgcacagtt agattcactt gagtcattat cacgtgatga aattgctaat
     3301 gatcgctttg aaaaattcag aaatatcggt tcttatatag aataatcaac ttgagcattt
     3361 ttatgttaaa tcgatactgg gttttaccat aaattgaagt acattaaaac aataatttaa
     3421 tatttagata ctgaatttta actaagatta gtagtcaaaa ttgtggctac taatcttttt
     3481 ttaattaagt taaaataaaa ttcaatattt aaaacgttta catcaattca atacattagt
     3541 tttgatggaa tgacatatca atttgtggta atttagagtt aaagataaat cagttataga
     3601 aaggtatgtc gtcatgaaga aaattgcagt tttaactagt ggtggagatt cacctggaat
     3661 gaatgctgcc gtaagagcag ttgttcgtac agcaatttac aatgaaattg aagtttatgg
     3721 tgtgtatcat ggttaccaag gattgttaaa tgatgatatt cataaacttg aattaggatc
     3781 agttggggat acgattcagc gtggaggtac attcttgtat tcagcaagat gtccagagtt
     3841 taaggagcaa gaagtacgta aagttgcaat cgaaaactta cgtaaaagag ggattgaggg
     3901 ccttgtagtt attggtggtg acggtagtta tcgcggtgca caacgcatca gtgaggaatg
     3961 caaagaaatt caaactatcg gtattcctgg tacgattgac aatgatatca atggtactga
     4021 ttttacaatt ggatttgaca cagcattaaa tacgattatt ggcttagtcg acaaaattag
     4081 agacactgcg tcaagtcacg cacgaacatt tatcattgaa gcaatgggcc gtgattgtgg
     4141 agatctagca ttatgggctg gattatcagt tggtgctgag acaattgtag ttccagaagt
     4201 gaaaacagat attaaagaaa tagctgataa aattgaacaa ggtattaaac gtggtaagaa
     4261 acactcaatc gttcttgtag cagaaggttg tatgactgcg caagattgtc aaaaagaatt
     4321 atcacaatac atcaatgttg ataatagagt gtctgtgtta ggtcacgttc aacgtggtgg
     4381 tagcccaaca ggtgcggata gagttttagc atcacgttta ggtggatatg cggtagactt
     4441 attaatgcaa ggtgaaacag ctaagggtgt tggaattaag aacaataaaa ttgtagcaac
     4501 atcttttgat gaaatttttg atggtaaaga tcataaattt gattatagtc tatatgaact
     4561 tgctaacaag ttatctatat aagatttcag gaggaattat aaaatgagaa aaactaaaat
     4621 tgtatgtaca attggaccag cttcagaatc agaagaaatg attgagaaat taatcaatgc
     4681 tggtatgaac gttgcacgat taaacttttc acatggtagt catgaagagc ataaaggtag
     4741 aattgataca attcgtaaag tagctaaaag attagacaaa attgtagcaa ttttattaga
     4801 tacaaaaggt ccagaaattc gtacgcataa tatgaaagac ggtatcattg aacttgaacg
     4861 tggtaacgaa gttattgtta gcatgaatga agttgaagga acacctgaaa agttctcagt
     4921 aacatatgaa aacttaatta acgatgttca agtaggttca tacattttac ttgatgatgg
     4981 cttaattgaa ttacaagtta aagatattga ccatgctaaa aaagaagtta aatgtgatat
     5041 tttaaactct ggtgagctta aaaacaaaaa aggtgttaac ttacctggcg taagagtaag
     5101 tttacctggt attacagaaa aagatgctga agatatccgt ttcggtatta aagaaaatgt
     5161 tgacttcatt gcagcaagtt tcgtacgtcg tcctagtgat gttttagaaa ttcgtgaaat
     5221 tttagaagaa caaaaagcta acatttcagt attccctaaa attgaaaacc aagaaggtat
     5281 tgataatatt gcggaaattc ttgaagtgtc tgatggttta atggttgcac gtggtgacat
     5341 gggtgttgaa attccacctg aaaaagtacc aatggttcaa aaagatttaa tcagacaatg
     5401 taacaaatta ggtaaaccag ttattacagc tacacaaatg ttagattcta tgcaacgtaa
     5461 cccacgtgct acacgtgcag aagctagtga cgttgccaac gcaatctatg atggtacaga
     5521 tgcagtaatg ttatctggtg aaactgctgc tggtttatat cctgaagaag ctgttaaaac
     5581 aatgagaaat attgctgtat cagctgaagc agcccaagat tacaaaaagt tattgtcaga
     5641 tcgtactaaa ttagttgaaa cttcattagt gaatgctatc ggtatttcgg ttgcacatac
     5701 agctttaaac ttaaatgtta aagcaattgt agctgctact gaaagtggtt caacggcacg
     5761 tactatctct aaatatcgtc cgcattcaga cattattgca gtgactccaa gtgaagaaac
     5821 tgcacgtcaa tgttcaattg tttggggagt tcaacctgta gttaaaaaag gacgtaagag
     5881 tacagatgca ttgttaaaca atgcagttgc aacagctgtt gaaactggta gagtatctaa
     5941 tggtgattta atcattatta ctgctggtgt accaactggt gaaactggaa ctactaatat
     6001 gatgaaaatc cacctagttg gtgacgaaat tgctaatggt caaggtattg gacgtggatc
     6061 agttgttggt actacgttag ttgctgaaac tgttaaagat ttagaaggta aagatttatc
     6121 tgacaaagtt atcgttacta actcaatcga tgaaacgttt gtaccttatg tagaaaaagc
     6181 tttaggctta attacagaag aaaatggtat tacatcacca agtgcaattg ttggtttaga
     6241 aaaaggtatt ccaacagttg taggtgtaga aaaagctgtt aaaaacataa gcaataacat
     6301 gttagttacg attgatgctg ctcaaggtaa aatctttgaa ggatatgcaa acgtactata
     6361 atttataaaa aaacgtcttt ccatttatca acaatggaaa ggcgtttttt ggttcatctg
     6421 gtattttatg acgtaattaa taggttattt gataatgata gtgt
//
LOCUS       DAMZLE010000122         6365 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMZLE010000122 DAMZLE010000000
VERSION     DAMZLE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.123"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1631
                     /locus_tag="POB92_002172"
     CDS             <1..1631
                     /locus_tag="POB92_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI6579642.1"
                     /translation="KKLTIILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKIIR
                     HQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGEQ
                     MTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLFY
                     IIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRTL
                     TMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYNA
                     IKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDNS
                     DRLVLNDLNLEIYNGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGIL
                     SQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEMLS
                     GGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRDN
                     TIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1628..3301
                     /locus_tag="POB92_002173"
     CDS             1628..3301
                     /locus_tag="POB92_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDI6579643.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLVVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKNRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFADEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3428..3871)
                     /gene="mgrA"
                     /locus_tag="POB92_002174"
     CDS             complement(3428..3871)
                     /gene="mgrA"
                     /locus_tag="POB92_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDI6579644.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            4098..5024
                     /locus_tag="POB92_002175"
     CDS             4098..5024
                     /locus_tag="POB92_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6579645.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIETTGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDRLERIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFSGPIDRQLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            5127..6035
                     /locus_tag="POB92_002176"
     CDS             5127..6035
                     /locus_tag="POB92_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDI6579646.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRSSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            6070..6357
                     /locus_tag="POB92_002177"
     CDS             6070..6357
                     /locus_tag="POB92_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579647.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
BASE COUNT         2158 a          882 c         1129 g         2196 t
ORIGIN      
        1 tgaaaaaatt aacaataata ctgtttcaat ataaaatttt tccggtactc atgttcttgg
       61 tcagtactgg tctcggcata ctcgttataa cgcaaaatat tttaatagca gattttttag
      121 ctaaaattat aagacatcaa tttcaaggtt tatggattgt attatttatt ttattaggtg
      181 ttttactttt aagagcaact gtgcaatttc taaatcaatg gttaggtgat acattagcat
      241 ttaaagttaa gcatatgctt agacagcggg ttatttataa aaataatggt catccaatcg
      301 gtgaacaaat gactatactc acagaaaaca ttgatggttt agcacctttt tataagagtt
      361 atttgcctca agtgttcaaa tcaatgatgg ttccgctcat cataatcatt gcaatgtttt
      421 tcatccattt caataccgca ttaattatgt taataactgc accatttatt cctttgtttt
      481 atattatttt cggtttgaaa acgcgagatg agtcaaaaga tcaaatgact tatttgaatc
      541 aatttagtca acggttttta aatattgcta aaggtttagt gacgttaaag ctatttaatc
      601 gtacagagca aacagagaag catatttacg acgatagtac tcagtttaga actttaacaa
      661 tgcgcatttt acgcagtgct tttttatcgg gattaatgct cgaatttata agtatgttag
      721 gtattggatt ggttgcattg gaagcaacgc taagcttagt agtatttcat aatattgatt
      781 ttaaaactgc ggcaattgcg attattttag cgcctgaatt ttataatgca attaaggact
      841 tagggcaagc gttccatact ggaaaacaaa gtgaaggtgc cagtgacgtt gtgtttgagt
      901 ttttagaaca accgaacaat aataatgaat ttctattaaa gtatgaggaa aatcaaaagc
      961 catttattca gttaacagac atatcatttc gatatgataa ttctgataga ttggtattaa
     1021 atgatttaaa tttggaaata tataatggtg atcaaattgc acttgtaggt ccaagcgggg
     1081 caggtaaatc cactttgaca catctaattg caggtgttta tcagccaaca ataggtacta
     1141 taagtacaaa ccagcgtgat ttaaatatag gaatacttag tcaacagcca tatattttca
     1201 gtgcttctat aaaagagaat attacgatgt ttaaagatat agaaaataat actattgaag
     1261 aagtgctaga cgaagtaggt ttattagaca aagtgcaatc tttcacaaaa ggcattaaca
     1321 caataatagg tgaaggaggc gaaatgttat ctggtggaca gatgagacgc atagaacttt
     1381 gccgtctttt agttatgaag ccagatctcg ttatatttga tgagcctgca actggtttag
     1441 atattcaaac agaacacatg attcagaacg ttctgtttca acattttaaa gatacaacga
     1501 tgattgtcat tgcacataga gataatacaa ttcgccattt acaacgacgc ttgtatatag
     1561 aaaatggaag actgattgct gatgatcgca atatttcagt aaatataaca gaaaatggtg
     1621 atgacttatg aaaacacgac taaaatttca agtagataag gatttattgt tagctatagt
     1681 tgttggtgtt tgtggaagtt tagttgcgct cgccatgttt ttcttaagtg gttatatggt
     1741 gacacaaagt gcacttggtg cgccactata cgctctgatg attttagtcg ttacagtaaa
     1801 attgtttggg tttttaagag ctattactcg atacgtagag cgccttattt ctcataaagc
     1861 tacatttaca atgctacgtg atattcgggt acagtttttc ggtaaattag taaatgtcat
     1921 tcctaatgtt taccgtaaac tgagttctag tgatttaatt tcacgtatga ttagtcgtgt
     1981 tgaggcatta caaaatatat atttacgtgt ttattatcca ccagtcgtca tcggtttgac
     2041 agcgctagtt acagtcatag ttttggcgtt catttcaatc ggccatgcgc tattgattat
     2101 ggttagcatg ttgtttactt tactcgttgt tccttggtta agctcaaaaa aagcacgtac
     2161 tttaaagaaa catgcagcta atgaacaggc ccgattttta aatcattttt atgattataa
     2221 agctggtatg gatgaactac gtcgatttaa tcaaattaat cattatcgag ataatttgat
     2281 ggctaaatta aatcattttg ataaattaca acttaaagag caacgctttt taacgattta
     2341 tgattttata ttaaatatta ttgctatgct ttcgattttt ggtagtttag ttctaggatt
     2401 aattcaaatt aatgcaggcc aactaaatat tatttatatg acgagtatag ttttaatggt
     2461 cttaacttta tttgaacaag ctgtaccaat gacaaatgtc gcgtattata aagcggatac
     2521 tgaccaagca ttgcatgata ttaatgaagt gatatctgta ccttctacta atggaaaaaa
     2581 ccgtcttaat gataagtatg atgcaacgaa catttatgaa gttaaggacg ctagttttaa
     2641 gtattggaac cagcaaacgt atgtgttgtc ggatattaat tttaatgtta atagaggcga
     2701 aaagattgcg attgtggggc cttctggttc aggaaaaagt acattactac aaattatggc
     2761 tgggttatat caattagata gtggctctat tcgtttcgaa aatatggata tgtttgaaat
     2821 agatgacaaa gataagtttg aatcgttaaa tgtcttgcta caatctcaac aattatttga
     2881 tggtacaata cgtcaaaatt tatttgccga tgaaaaagat gaagcggtgc aagcaatatt
     2941 taagcaatta gatttagaac atttggcact agaacgtcaa attgacttag atggtcatac
     3001 attatctggc ggagaaattc agcgtttagc gattgcaagg atgttattaa aagatactgc
     3061 atcaacatgg attttagatg aaccaacaac tgcattagat aaacaaaata gtttaaaagt
     3121 tatggattta attgaagcac atgcagaaac attaattgtt gctacacacg atttaacttt
     3181 attgtcacgt tttgagacca tcattgtgat gataaatggt aaaatagttg aaaagggaaa
     3241 ctatcaacaa ttactcgcta atcaaggtgc tttatggaat atgattcaat ataatgcata
     3301 aaaaaactgc ttgataggct aagaaacctt tcaagcagtt ttttaactaa caagcttacg
     3361 cgtgctaatt gtttttctat tcttaataaa ttctaaacat tactttaatt gtcatgacaa
     3421 aagttaatta tttttccttt gtttcatcaa atgcatgaat gactttacct aataagcgat
     3481 taagttcttt aacttcatct tgagataaag aagaagctga agcgactttg tcagatgcat
     3541 tacttaattc tggtctaata gtttcacttt tgtcagtcaa gtgaataaat acttcacgtt
     3601 gatcgacttc ggaacgttca cgcttaatta agtctacttg ttccattcgt tttaataatg
     3661 gtgatactgt accagtatcg agtgctaatt cagttacgac tttcttgacg tttacaggag
     3721 attcatccca taaaattgtt aagacaagaa attgtgggta tgttagattg tacttcttaa
     3781 aaactttgtt agagtagtag cgattaactt gtctttgagc attgtacaaa ctaaagcata
     3841 gctgttcttt taaattatgt tgatcagaca ttaaagttct cctccagaca tactatccgt
     3901 ttttttctct tttcggattg gtaatcatta aaaagttgat tgtttattaa ttcacaactt
     3961 tctttgattc aatgccatgc taaaattaaa gtatgtttaa agtttagaag atatttttga
     4021 ttaaatcaag caaaaagata atttaatata tatgttatca tttttaaaaa taactgtaat
     4081 agaaaagaga atataaaatg aaaaataata aagatgaaaa aataagaata tccataatta
     4141 acggattttt gggtagtggt aaaaccacgt tactgacaca ttatattagt gaattattaa
     4201 aaaatgatga gaaaattaaa atcatcatga atgaattcgg tacttttgat attgatagca
     4261 atagtatttc aaatgaaatt gaagtccatt cattgattaa tggttgtgtt tgttgcgatc
     4321 ttaaacaaga acttgtctat gaactaaaag ccattgcttt aaaaggggac gttaatcatg
     4381 tcatcataga aacgacaggc attgcgcatc ctttggaatt actagttgca tgtcaagatc
     4441 cgcaaatcgt taatttcttt gaaaagccga ttatttatgg tgtattagat gcgactcgat
     4501 ttttagaacg tcatcaatat accgaaaata cagtttcgct gatggaagat cagttgaaac
     4561 taagtgacat gattattatt aataaaattg atcttataac tgatgaccgt cttgagagaa
     4621 ttgataagca attaggtatg atttgtgcaa gtattccaac atataaaaca acctatggaa
     4681 aagtttcgtt ggaagaattg gacttaactg ttaaagacag agagatatcg tctcatcatc
     4741 accatcatca tgggattaaa agtatgactt acacgttttc aggtccgatt gatcgtcaat
     4801 tgttttatca atttataatg aaattaccgg aatctgttct acgtttgaaa ggttatgtgt
     4861 catttagaga tcaaccaaat gcaatttatg aatttcaata tgcatatggt ttaccagact
     4921 atggaataat tggcatgcaa ttaccattaa cgattgttat tattggtgaa actttagata
     4981 caaatcacat acgtaatcaa ttggatatgc tacaatttac gtaaatcaga aaatgtgttg
     5041 tttcaattta acggaaatag cacattaatt tcttaatact tttattgtga atattttgtg
     5101 acattgaggg atggagtgga tgagatatgg aacaaataat gattaatcac tatgttcatt
     5161 tttctaggct tgtacaaggt ttttggcgtg caaatgaatg gaagatgact gcgaaagagt
     5221 taaattattt tataaatgaa ttagttgaac gtggaattac aacgatggat catgctgata
     5281 tttatgggga ttatcaatgt gaatcactgt ttggtaatgc tttggattta tcacccgaat
     5341 taagaaataa aattcaaatt gttacgaaat gtggtatcat tttgccttct aagcaatttg
     5401 attttacaaa tggacatcgt tatgatttga gtagtaagca catcgtgaaa tctgttgaac
     5461 agtcattaat caatttgaat gtagattatt tagatagtct actcattcat cgttcttcac
     5521 cattgatgga tccagaacaa gttgctgatg cattaactaa acttgttaaa caaggtaagt
     5581 tgaagtcatt cggggtgtcg aattttaatc attcacaata ccaattgtta aatcaatata
     5641 ttatgaaaga aagactacat attagcatca atcaattaga attatcgcca tatcacgttg
     5701 atagtttaca agatggaaca atggattcaa tgtatcaaaa ccatgttcaa attatggctt
     5761 ggagtccttt tgcaggcggt aaaattttcg acaaggaaga tattaaagcg caacgtatta
     5821 tgaaagttgt tcaatcaata gctgacaaat atggtgtgag tgacacagct gtgatgatag
     5881 catggttagt aaaaataccg catcgtatca tgccgatact tggaacaagt cagttaaagc
     5941 gtattgatca agcaatcgaa gggctacaac ttaatttaga tgatcagtcg tggtttgaca
     6001 tttacaccgc tattatcgga caagatattc cgtaaactta tttactttta aatcataaag
     6061 gagcatatca tgacaaacga agataaacgt ttcgaacaat taagatttga acgcaaattt
     6121 atagttattc cgtatttaat ttatgcagtc attgtattac tattaaatat tttctattct
     6181 gatttgaaaa taacaatgac attattcgga cttttctttg cgtataatgt agtcattttg
     6241 ttcatagcat ttattaaaca ttataaacgc acattgttac taagtcttat attaacagtg
     6301 cttagtggcg cggcattctt tggaattatt tatgtttatg gcattaatca tttttaaata
     6361 ttaaa
//
LOCUS       DAMZLE010000123         6283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMZLE010000123 DAMZLE010000000
VERSION     DAMZLE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.124"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(19..897)
                     /locus_tag="POB92_002178"
     CDS             complement(19..897)
                     /locus_tag="POB92_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDI6579648.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSESLFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(917..4357)
                     /locus_tag="POB92_002179"
     CDS             complement(917..4357)
                     /locus_tag="POB92_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDI6579649.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTMINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLKQMDRSYVEMITEFETNKADISRRQQRLTVYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4660..6000
                     /locus_tag="POB92_002180"
     CDS             4660..6000
                     /locus_tag="POB92_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6579650.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
BASE COUNT         1974 a         1017 c          956 g         2336 t
ORIGIN      
        1 ataaaatttt atactttttt aaaaatggga tgaaatatat tttcctgaaa catgtaattc
       61 atgttcgtta caaattgata aaatatgatt aagtgttgtt gcaaatgaca ttttctcaaa
      121 aagcgattca ctatcaattg gtacttgtgt gtgaatttca gcaagtcgct tcgataaata
      181 tagttcatct aaattgtcat taatcttatt acgttgccca gctgataatg catcaatgtt
      241 ttcaaccaca ttttcaacgc tttgatattg ctgaattaac ttaatagccg ttttctcacc
      301 aattccttta acacctgcat atccatctgc tgtatcaccc ataaacgctt taatatcgat
      361 tagttgttgt ggttcaaggg catattcttc gttaaaacga tgtaatgtat atctattata
      421 aatgttaaaa ccttttttaa ttagccagac ttcaacattg tcattaatac attgcagtaa
      481 atctttgtcg cccgtaataa tatagacatc gttatcagtt gaatattgtt gtgctaatgt
      541 acctataaca tcatccgctt catagttttt aacgccaata tttacaaagc caaattgctc
      601 tgaaatttct ttaacataat caaattgtgg tatcaattct tctggtggtg cagaacgatt
      661 ttgcttataa ccatcaaaca tatcatttct aaaagttgat tgtcccatat cccaacatac
      721 agctacatgt gtagggcgta tttcatgtat tgccgaaaag atatgacgca caaacccttg
      781 tattccattt gtaggtacac cttgtgaatt gtacataaat tgtttatgaa gacttgtagc
      841 gtagaaatgt ctaaataata gtgccatacc atctacaagt aatattttat taggcattta
      901 aaacgttcct tctttcttaa tataaaatag ttttcagttg ttgatgttta tctttcaatt
      961 gattgataag tgttgtatct aaatcaaatg ctaataaatc gtttatttgc gtttgaatat
     1021 tagcttcaaa actttcaaat tgcgattcag cttctacagc catttgctta acatataatt
     1081 caagttgttg cctcaataat cccatttgtg gttgtaataa tcctaatgta ctttgacaaa
     1141 ttgattcatg tatatctctt tgcccatttg gattcaaaat tttacgtttt gttaattgtt
     1201 taggcaatgc atttagcata tcattgaaat cgatgtgcaa taatggttgc tctatattag
     1261 ctgattcaaa gttaaactga ggattaacaa tgacatgtaa atctgctaat tgttgaacga
     1321 ttggtgcaat ttgctcagtg agttgcttat taaagtattt ttttatacgt tctgtaatta
     1381 aagattgctc taaaaacaat cgttgatgaa tttgatctaa gtatactttc gtagacactt
     1441 tcttttcttc attaaaatca ctattttgcg tcatttgaga attaaacact gatttaacat
     1501 cgtcaagtag ttgtaatttt aaacgtgcat ttaaatgata aacttgttct tcaacttcgt
     1561 tgtctgtatg ttgtaacgtc gcatcaatta attgatgttg taaacgctgt ttatctttat
     1621 agactgttaa cctttgttgg cgacgtgaaa tatcagcttt atttgtttca aattctgtaa
     1681 tcatctctac ataagaacga tccatttgtt taagctgatg aatcatttgt tgttctaaaa
     1741 ttgatttaga ttcaacatca acaaattgtt gtatgctttg ttttaattga tcaatgccct
     1801 tatcttcagc ttgtaatgca tttcgacttg atacagcaaa aatgtctgat tgtaagtgta
     1861 cttgtcttaa tgcatctgat acatatgttt caaccgcttc aagatcatct tgactttctg
     1921 ctaaatcagc agcattaatt accattttaa atgcttggtt ttcattcaac tggttcatat
     1981 ctttcatgtg ttctataaac gccttgtcat tatcagtaaa tgaatgatta aaataactta
     2041 catacaatat taagtctgaa gaagttaaaa tttgctcggt ttcatttgta tgcctttggt
     2101 tatttgagtg tagccctaat gaatcaacaa ttattttacc ttttaaccaa tcatgcatta
     2161 atgcaatgtg tactgtctta acaaatgttg catattcatc ttctgcactc cactttttca
     2221 attcctgttg attaatggca tgtttttctc cattttccaa catattgaca tacaatttat
     2281 aatgtttttc aactgcctga ataaaagcga gttgattttt atttaaatgt gattttaact
     2341 tttctaaatc tgaattaata aagtcttcta tagtcgaata agacatatct tggtattcaa
     2401 ctactgcatt aatttcatct aataattgcg attgtgattt tagcgttata taactctcgt
     2461 ctccataaga tatttctgta gtagctgctg tagtaggatt tggagaactg actaaaatat
     2521 gctcgcctaa taatgcattt atcaaactac ttttaccagc actaaatgtt ccaaatacac
     2581 ctatttttat taatttatta tctatacgtg ttaatgtttc gtggatatct tgctttgtac
     2641 gcttgaacaa aggcacatcc gaaattatat caagcgcttt ttgaatatct atagacatat
     2701 ttgttgtagc tgtattttga tttagctgct gattatcacg attatcctga gtattatcag
     2761 tagccacttg atatgttgtc tcttgtcgac ctattaattt atctagagat tcatctaaat
     2821 gaatatagta gtgacgataa ttcttagtcg tcaatgactg acgcagctca tttaattctg
     2881 tataacgttg atattctttt aaatcatcac tttcttctgt tggtaattca tctgcctgta
     2941 cattttctat tatttcttta aaaattggtg ttgattgttg ttcaacatat ttcttaatgg
     3001 ctttaaccac ttcgtctgaa aatgtaagta cataagtatt gctgattgat gtttgtggtt
     3061 gatataaatc ttcaatcatt tccggcttaa cgtcataatg ctgatttaat actttatctg
     3121 aagcttcttt tttattgata aaacgcgtta caaatgacat atcttctcgc attggttgac
     3181 gaatttgttg attaacatgt tcttgtaacg catctgtcgc tgttaataat cgctgttgtt
     3241 gaatttgtag ctttttcttc tttttattaa aaaacccacc aacattaaag tcttgagaca
     3301 tactttccaa ataacttcgt aacatttctc tcatattatg cggcataatg tatgcatttt
     3361 ctaaaatatt tttacgctta ttctttaaaa atgccattaa ttcatctgga ttatttaaaa
     3421 gttgtgcctc ttcactaatt gcttgatgtt gttgactatt taaaaatgct tgttcaaact
     3481 ccgcttcttc gatacctaaa tcttctagta cttcctgaat ttcagactga atgtaatcta
     3541 gctgagcttc ggtaatgtat tcaaccgttc ttgatgtata atcctctatt gtctctctat
     3601 gttgatctaa tgaaattaga taacttgata atacttcaag ttcattttca gggtgatcaa
     3661 atttagatac ataaaaggtg cgttctaatt taatacccca atctgcaatt gatttttcaa
     3721 ctcgagattt aaacgtagag aatgacaatt catcgtcttg atgcttgtca atttgattaa
     3781 tgataaacac aacaggtatt ccaacatcat ttatatgctt cataaactta aagttaagtt
     3841 cagattgaac gtggttatag tcaaccgtat aaaatatcat attacttgta tacatatatt
     3901 gttctgttat tgactgatgt gatgcaacat ttgaatcaac acctggtgta tcttgcaacg
     3961 taaacccatt ttcaaattta gctgattgaa aattaatttc tacagattca acgtcgacat
     4021 tttggcgatt catttccctt acttcatcat aattagataa tttggcatac gtttgattcg
     4081 gcaaattagc aataatatcg tgattgtctg aaactgacac aatagcagta ttacttgtcg
     4141 ttggtacagg agaacttggt aagatatctt gttcaattaa taaatttatc attgtcgatt
     4201 tacctgcaga aaaatgtcca acgaacgaac atgtatattg ctgcaaatat actttcttaa
     4261 ttacttggtt aattgtatgt aaaagtgctt catttcgcga cttttcaact tcttttttta
     4321 atttatataa aagatctaat tgttctttat taatcatgtt actttcccct ttggttcgtt
     4381 cacatagaaa tgcttgcttg ttacaacgaa ttcatacaat atcgatgtcc atttaaacat
     4441 agatcgttaa atggctcgca ttgtattatg tttaaatttt atcatagctg cttgataaaa
     4501 catacgaata atcaattttc ttcaaacatt ccaacttaaa agttcatttt ttaaaaatca
     4561 caaatcatcg ttaaattttt ctagaaaata aatattgtca cagtttgttc acacacgtca
     4621 cagaattgac accgtataat agccaattgg gagggaaata tgaaaaatac atttaatcca
     4681 ttacaaagat tacatttcta tgcagcaata tttattgctc cactgttaat cactttaacc
     4741 atttcgggca ttggttactt gttctttcca gaagttgaaa ataatattta taagaatgag
     4801 ttttttggtg acagtgatgt aaaaacgcat caaacattaa atgatgcagt acatcaagtt
     4861 gaacaacaat atgaaggatt ctttgtaagt aaagttagca tacttgatga accatataac
     4921 aaacgaatta cactgagtga tatggcagga aatcaacgtt acgtctttct agatcataac
     4981 aatcaatttg ttgcagatca aaatgcgaaa catacgtatt ctaatgtgat gcgaagtata
     5041 catagttctt tgtttactga aaatactatt attaattatt tagtagagtt aaccgcatgt
     5101 tggatgatat tcatgatttt atctggtact tatttactca ttaagaagca tttaatttct
     5161 aacaaaagta aggcacttcg ttggcaaaag tggcacgcaa tgattggagt tatcattgca
     5221 attccagtat ttgtattagt cttaactgga ttgccatggt ctggttttat gggcagtaaa
     5281 attgccggta tgatggacac aaacggtgac cttggtcaag gtgaattagc gattaatcca
     5341 cctaaatcag atttgaacga attaccttgg gctacacgta aaaataaaca gccagcttca
     5401 tccgaaaaag gttcaagtgg tcatcatggt aatgcagcaa tgcctcaaac caaattagat
     5461 tatcaaatat ctattgataa ggtcgttgaa caggcgcaaa aagctggtat taaaaagccg
     5521 ttttcaatcg tatatccaag tgataaaaat ggtaccttta ttgtatctaa tactagtaat
     5581 tcaggtgtta ctgggctaga tgtatcacca tacaaggaac aaacacttta tttcgatcaa
     5641 tatagcggta aaaagctagg tacgattaaa tatgatgact acggtattat tgctaaatgg
     5701 tttacatggg gcattccgct tcacgaaggt catttattcg gcattttaaa taaaatcatt
     5761 aatttatttg tatgtatcgc tttattagta gccattggca tggggtttgt ctcttggata
     5821 aagcgtacaa aaaatactgc agtaaaagta ccacatcgcg taaaaaaacc agcatctata
     5881 tcactcataa tatgtttaat tgtattagga ttattaatgc cattatttgg attatcactt
     5941 atccttgtat ttataattga attaatatta tatattaaag atcgtcgtgc taaacaataa
     6001 tgcacttaaa gttttgaact gacgaaattt acaaaatgat tctcgtctct ctaattactt
     6061 aaaacgggtt ctataataaa tcgtactgat ggagcttggg agataaatca atgtcctagg
     6121 gtctataata ttatattggc agtagttgac tgaatgaaaa tgcgcttgca acaagctttt
     6181 ttcaactcta gtcaggggcc caaacacaga gaatttcgaa aagaaattct acaggcaatg
     6241 cgagttgggg tgtgggcccc aacacagaga atttcgaaaa gaa
//
LOCUS       DAMZLE010000124         6266 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMZLE010000124 DAMZLE010000000
VERSION     DAMZLE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.125"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..357
                     /locus_tag="POB92_002181"
     CDS             <1..357
                     /locus_tag="POB92_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579651.1"
                     /translation="TNENDLKKRLKKITNHDDYFLLLLIKENKIIGLSGMCKMMFYEK
                     NAEYMRILAFVIHSEFRKKGYGKRLLADSEEFSKRLNCKAITLNSGNRNERLSAHKLY
                     SDNGYVSNTSGFTKQL"
     gene            complement(1211..2518)
                     /locus_tag="POB92_002182"
     CDS             complement(1211..2518)
                     /locus_tag="POB92_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI6579652.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            2679..3590
                     /locus_tag="POB92_002183"
     CDS             2679..3590
                     /locus_tag="POB92_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6579653.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            3652..4497
                     /locus_tag="POB92_002184"
     CDS             3652..4497
                     /locus_tag="POB92_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6579654.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSDILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(4869..6092)
                     /locus_tag="POB92_002185"
     CDS             complement(4869..6092)
                     /locus_tag="POB92_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDI6579655.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIKYLKEK"
BASE COUNT         2320 a          814 c         1002 g         2130 t
ORIGIN      
        1 acaaatgaga atgatttaaa aaagagacta aagaaaataa cgaatcatga tgattacttc
       61 ctactgcttt tgataaaaga aaataaaata attggtttaa gtggtatgtg taaaatgatg
      121 ttttacgaaa aaaatgcaga gtatatgaga atccttgcgt ttgttataca ttctgaattt
      181 aggaaaaaag gttatggaaa gagattatta gctgattctg aagaattttc taaacggttg
      241 aattgtaaag caataacact aaatagtggt aatagaaatg aaagactatc tgcacataaa
      301 ctatatagtg ataatgggta tgttagcaat acatctgggt ttactaaaca actataagtg
      361 tgagcattcg tataatacga tgtaggaaca tattttattg taaatgttgc atgtatgcct
      421 cgtattatga cttatttaaa gggagaaaaa agggcataat tcttcgattg ttggagaaat
      481 ggactagatt ttcgagattt tccaacaata aatcaatgtt ggaaaacagt ttttgagtga
      541 cataattatg acataataga attgaaaaac taaaaataat agcgttgtct aaaaattaaa
      601 acgctgattt aataatcttt aaagcgtgta ttaaagtaag tttttattag tgcataatgc
      661 cgggaatgat gtaaaactgt atatagtttt gactaattag aaaattacat tatgaacgag
      721 tatgatgtat tctaagtaca aattagaatg cacgcaaata tattttaatt acgagacgtt
      781 ttccatttgg tgactagact tatgggaggc gtctttttta tgtgctaatt tgtaattgag
      841 attcataatg gtatttaaat cattatggtg cgggagttat ggtattgcca taatatgaga
      901 gtgcatccac ttctataagt aatgcatatt gcgagtgcag gaatgaatga gtgcttgatt
      961 aaaatcctta tgggtggttg acataattaa aagaaaccac atttaaaatt tcttaatcac
     1021 aagcggttaa ctaggtatag tttagttttg agtaaatctt tttaaaggtg tacttgtgca
     1081 ttttacttaa ttaaagacat aagacattta atgggcctaa aataaataaa aaactacctg
     1141 tttaggtagt ttttttaatg taatagatta aaacactagt tcatttcttg ttaaagatgg
     1201 ataagctatt ttatagataa atttgtcctt tagtgtagcg gtaattttta ggactttttg
     1261 gtggtataaa tgttcttaat aaagttaata gtcctacttt accgcaaagc ataacgaata
     1321 taataattat tttagtaata ccatgatatt ctgtggtaag gttcatactt aacccgactg
     1381 ttccaaatgc agaaaccact tcgaataata acttgattaa tgatatgttc ggattaatta
     1441 tcgataatat aaaagtaatg atactgataa atagaaatga gatattaatg gtaacaatag
     1501 atagttttat atgtttgtca gatatttctt tattgaatac tgaaacatta ttttctttac
     1561 gtatataatt tagaacaaat ataaacgcca ctgcaaaagt agttatttta attcctccag
     1621 ctgcactgag aggggcacca ccaataaaca taagtagcat taacattaag gcggtagatt
     1681 tgttaatgct tgctatatct atactgttaa aacccgctgt tcgtgttgtt actgattgga
     1741 aaaaagaatt tccgattttt tcaactagtc ccatatgttg catagtatta aactgttcta
     1801 ataaaaagaa tgtaatagct cctataatta ttaggatact agttgtagtt aagactaatt
     1861 tagaatgtaa agataattta ctcaattttt tacaattaat aaagtctatt acgacaaaat
     1921 gtccaatacc tccaaatatt atgagtattg agattgtaat aatgacaatt ggatcactag
     1981 aataatctat taagttattc ttaaaaaggg caaatccggc attattaaaa gctgatactg
     2041 atgtgaataa acttaaaaat aatcctttgc ctataccaaa ttttggtata aaagataaac
     2101 acaaacaaat cataccaatt aattcagtga ctaaactata aatagccaag tgtttaatta
     2161 gcttaataac accaccaggt tcgtcaatat tccatgtaac cataatcaag aatctatttt
     2221 tcattgatat ctttctattt aaaaatacta gtgtcaatag ggttacggtt acgataccca
     2281 gaccacctat ttgaattaat aatagtatta ctatttcacc aagtatatta aactgtgacc
     2341 ctatatcaac tggagatagg ccagtaactg taaatgcact tgaagctata aatagggcat
     2401 ctaaaaaaga tattggcttt ttaccagtga aaggtaaata caataaaagt gcacctatga
     2461 tagttgtaga gaagaatagc attaaataaa aatataaagg tttgtggact ttgttcattt
     2521 taattgcata ctcctttata ttataaatta ataattagat aatatcataa atgaaaatag
     2581 aaatgttact gatgtgtatt actaacaggc gttgactaat tcttttaata ttttataatt
     2641 gaaaatgaat atcaattaaa aaacaaaagg atgatattat gaaaagatta attggaattt
     2701 tattatgtaa tttatttatt ttgactgcgt gttcagcatc tgtcgataaa acaagtaact
     2761 cgacaaagac tacagactat aaaattgaaa atggtgaaac actgaaagta ccagagaaac
     2821 ctaaaagagt tgctgtatta actggatttt atgttggtga ttttataaag ttaggaatca
     2881 aaccaattgc tgtttcagat ataactaaag attcttcaat tttaaaacct tatttaaaag
     2941 gggttgatta tattggagaa aatgatgttg aaagagttgc taaagcaaaa ccagatttaa
     3001 ttgttgtaga tgctatggat aaaaatataa aaaaatacca aaaaatagcg ccaacaatcc
     3061 catatacata caataaatac aatcataaag aaatattaaa agaaataggc aagttgacta
     3121 ataatgaaga taaagctaaa aaatggattg aagagtggga cgataaaact agaaaagata
     3181 aaaaagaaat tcaaagtaaa attggtcaag caacagcatc tgtgtttgaa ccagatgaaa
     3241 agcaaatata catatataac tctacatggg gtcgtggttt ggatattgtt catgatgcat
     3301 tcggtatgcc aatgacaaag caatataaag ataaattaca agaagataaa aaaggctatg
     3361 cttccatttc aaaagaaaat attagtaaat atgctggtga ttatatattt ttaagtaaac
     3421 cttcgtacgg aaaatttgat tttgaaaaaa cacatacatg gcagaatatt gaagctgtaa
     3481 aaaaaggaca tgtaatttca tataaagcag aagattattg gttcacagat cctattacat
     3541 tagaacattt gagaagtaaa ttaaaaaaag aaattttaaa taaaaaataa tagaaataag
     3601 ttgtaaaaat tttcttatgc attggtacta atgtttttaa ggagtgatta aatgaagcaa
     3661 ctggttggaa ttcccgaatc aatgttaatt cctttgatag ctcgagcaaa agagtacgaa
     3721 aacgaaaaac caataataaa agacgcacta tctaaaaaaa tatttgatgg tttagatgat
     3781 atgtacaaaa atgttacatg tgatgacatg tctcaaattg gaattagtat acgtactgtg
     3841 ataatagatt gtgttactaa gaggcttatc aaggataata aagatttaat cgtggtcaat
     3901 ataggttgtg gcttagatac aaggtttcaa agatttaata aagaaaaaat atcatggata
     3961 gatttagatg taccagaatc aatagaaata cgaaaaacat tttttaaaga aagtaatagt
     4021 tataagatga tatctaaatc tatgctagat tacagttgga ttgatgatgt caaaaattat
     4081 aaatttttta atagtaagtc agatatattg tttattattg aaggtgtatt gatgtatttt
     4141 gatgagagtg taatgactca attattggac actattatca aaaagatggg agatcataat
     4201 ttgacatttg cgattgaatt ttgctcaaaa acaattgcga ataatacaaa gagacatcaa
     4261 tcggtatcaa agttatcctc acaacctgtt tttaaatatg gttacaatga tttaaaaaaa
     4321 ttgaatgaaa ttttaccaaa tacaataaga gtaatacatg aatataatta ttttgactat
     4381 tataagaatc gatggggctt atttggatat tgtagattta taccttattt aaaaaaaagg
     4441 ttaaacaata aaatagtact tatgaaatat aaagttccaa agagacagag acattaagtt
     4501 cttagacaat gtaaaaaagc tgatttctat aaattatttg atagaaatca gcttttttca
     4561 aatgtatttg ataatttaca gctcgttgag ctgctgtttt ctagggattt atgtcccatt
     4621 ccttttacga gaaggttttt acgagtttta tgtaactatt agaagctatc tttttatatg
     4681 accattagtc aatatatgta tgacaaatca gcacaataat ttcagtttta catcacttaa
     4741 tcaatggaaa tgtgcttgtt gatatttgaa tatttacatt caaacaatat tcatcgttat
     4801 ttctatcaac ttaaaattcg caataaaaaa ctgccagcat gcgaataggg cagctgacag
     4861 catcgttctt atttctcttt taaatattta atggaagcct ctttaaaaat atcaatatat
     4921 ttcagataca tatctttttc aatatattca tcgatttgat gtgccattaa tggattacct
     4981 ggtccaaaaa tggctaaatc aacattgtcc ttattatctc ctaagaaact agaggcatct
     5041 gttgcaccta caagcgctga aacaaatatt tcgtcttgtt ctacataact agaagctaca
     5101 tctttaatcg tagtaattaa tttgctattt ttatcgcttg ttacaggtcg gtgattgctt
     5161 ggaatatcga gtgaaagctt attgctatcc acatcattaa tgatattttg gaaaaacgat
     5221 tctataaagt cgttatcata ctcaggaact ggtcttacgt taaattcaag tgaagcttca
     5281 tctggtacag agttaaattg tttgccgcca tttataatcg aacatacagc tgtaagacca
     5341 gatgcataat ttgcatcctc ttcagaaatt tcttttccaa tcaatgattt gaacataggc
     5401 gcaacatcta attcatgttt agtatcttgt tgtttaagct ctgaatattt ttctttaaat
     5461 agattataaa attcaagcag tgtatcaatt gcattgtcac caataaatgg aactgagcta
     5521 tggacagctt taccagttgc agttacttta catgacatag accccttatg tgcataataa
     5581 attccagatc cagttggttc agcaataatt aagccatcga catcgtctaa atagccttta
     5641 tcagctaata atttggcacc ttcttgttct ttctcttcgc cagcagtagc tagtaatctt
     5701 atcgttcctt gaggcaattg attttgttct tttaattcaa tgagtgtgat gaccaaagcc
     5761 atcagaccgc ctttcatatc cgttgtgcct cgaccgtata atttgccagc tttttctgtg
     5821 agttgaaaag ggggataagt ccaattatct tgatttcctg catcaacaac atccatatga
     5881 ccactcaatg cgagtatagg tgagccgtta ccgatttctg caacgatatt ggcgcggtgt
     5941 tcattaactt tcaaaatttc agatttaata tcgtacttgt cgaataaatc ttttaaataa
     6001 ttacaaacgt ctatttcatt attattttca gtttgtagtt caacaatatc tgctagtaat
     6061 tgaatttttt ctttttcact aaaagttgtc attaagctca caccttttca aagtagtata
     6121 tatatttata taaacataat ttttattctt aaacataatt taaaatgcag aaagtagcgt
     6181 gttatttgat ttcgttctat gaaattaact gtctagtatc aacgatctta ttaacactga
     6241 tatcgggtat gcttaatttt atagta
//
LOCUS       DAMZLE010000125         6263 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMZLE010000125 DAMZLE010000000
VERSION     DAMZLE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.126"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            108..836
                     /locus_tag="POB92_002186"
     CDS             108..836
                     /locus_tag="POB92_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDI6579656.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSSLDEV"
     gene            complement(1249..1610)
                     /gene="ssrA"
                     /locus_tag="POB92_002187"
     tmRNA           complement(1249..1610)
                     /gene="ssrA"
                     /locus_tag="POB92_002187"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1701..2165)
                     /gene="smpB"
                     /locus_tag="POB92_002188"
     CDS             complement(1701..2165)
                     /gene="smpB"
                     /locus_tag="POB92_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI6579657.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(2187..4559)
                     /gene="rnr"
                     /locus_tag="POB92_002189"
     CDS             complement(2187..4559)
                     /gene="rnr"
                     /locus_tag="POB92_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI6579658.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDINEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGSQRKGKNERKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(4593..5333)
                     /locus_tag="POB92_002190"
     CDS             complement(4593..5333)
                     /locus_tag="POB92_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDI6579659.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQAENDNMIDPQSANYIYDHVDSDDKDIKWYSQS
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(5449..5682)
                     /gene="secG"
                     /locus_tag="POB92_002191"
     CDS             complement(5449..5682)
                     /gene="secG"
                     /locus_tag="POB92_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI6579660.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(5749..6207)
                     /locus_tag="POB92_002192"
     CDS             complement(5749..6207)
                     /locus_tag="POB92_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579661.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
BASE COUNT         2099 a         1167 c          881 g         2116 t
ORIGIN      
        1 tatcatctta gaatctcaca aaatatcaac tttgtttaat tactcaaaat gataagtcat
       61 ttagagtttt taaaataaat ttttgcgaaa taaaggagac atgttaaatg aaaaaagtaa
      121 tggggatatt attagcaagt acacttatct taggtgcttg tggacatcat caagatagtg
      181 caaaaaaaga gagcactagt cacaaaaaga aagaaaatga caatgaagaa ttaaatgaag
      241 aacttaaaga atttaaaagc aaaaaaaata tggatataaa aattaaaggc gatactattg
      301 ttagtgacaa atttgaagct aaaataaaag aaccgtttat catcaatgaa aaagatgaga
      361 aaaagaaata tatcgctttt aaaatggaaa ttactgctaa aaaagacgat aaagacttaa
      421 atccatcttc tatttctcat gactatatta atatcactca agatgataaa aatacagtaa
      481 ataaattaag agatggttat cttttaagtg ataaaaaata taaagattgg acagaacata
      541 accaagatca aattaaaaaa ggcaaaactg cacaagccat gtttatctat gagttaagag
      601 gtgatggaaa tattaattta aatgtccata aatactcaga agataaaaca gttgattcta
      661 aatcattcaa atttagtaaa cttaaaaccg aggatttttc tcatagagcg gaaacaagag
      721 aagaagtaga aaagaaagaa aaagaatttg aagaagagta caaaaaagaa caagaacgag
      781 agaaagaaaa agaaaagcaa aaagatgacg accacagtag tttagatgaa gtataaattt
      841 atgaatgctc gcatacagcg ttataatctt tgtaattatt attgtcatta taaattagct
      901 tacctacact cataatgaca cactaattta attgttaaat acaacaaaaa agagtagctc
      961 gcctactctt ttttgttgtt ttgaatgaag aattatctta tataaagaat ttttttatga
     1021 acaatattaa aactctaagc tcaacttttt tgacacttat ttgacacata ttctttcaac
     1081 ccttaacgca aaatcttata tgtacacaag tattaatttt tcattcatga aaatttgaac
     1141 ctaagcaaat actatcatat caacgtacac agctaattta accttcctca caagaataga
     1201 taaaaaacat ataaaaaagc ccacatccac aaaggttgta ggctacaaat atggagacgg
     1261 cgggatttga acccgcgtcc agaggtcctg atacacatct ttctacgtgt gtagtttatc
     1321 gaaaggtttc gcatcatgaa aagtgataaa caaccaacat gatgctagct tgattaagtt
     1381 tcttctaaac agacttcaaa cggcagtgtt tagcatatcc tattaaggtt gaatcgcgtt
     1441 aacagcatat aggaaatgct gttaggcgat gcagagtgcg attaggcagc tactgcgaaa
     1501 ttattgtttg atttgccagt tattataaac tgtgtgtgtt gatgacgaag acaaccctcc
     1561 gacacgctta atgagctcgg gggacccctg tcgaatccat gaacgtcccc gaaataataa
     1621 atcaaacaaa tcatactttt gtaatttaca aaagcacata taactatatc aaatatagcc
     1681 cgattaagca actaaatcgc ttaataacgg gctttcatat cgcgcgcaac atctcgtttg
     1741 actgcttttt ctttcaaagc ttgacgttta tcatattttt tcttacctcg tgcgacacca
     1801 agtaatactt tacaatgccc atgcttcaaa taaagcttta atggcacaat cgaataacca
     1861 atttcacgtg tttgatcacc caatttaatg atttcacgtt tatgcaataa taattttcga
     1921 gaacgaagag gatcgtgatt aaaacgattc ccttcttcgt atggtgctat atgcatatta
     1981 ttcaaataca tttcaccgtt tttaacttgc gcgtaactat ctttaaggtt agcactacct
     2041 cggcgaatcg attttatttc tgtaccttgt aatacgattc ccgcttcaat cgtgtcttca
     2101 atattataat catgtcttgc tttacgattt tccgctaatg tacctggtga tttcttctta
     2161 gccattcata ctcacctcat tgctgcttat tttttcttac gacgtgcttt ctttttcaca
     2221 cttttatctt tataaaatgg cttatgttta ctatttcctg atttgtcatt tttgcgttca
     2281 tttttacctt tacgttgact accctttttc ttacgtcctt tatcatcaga ttttgattta
     2341 tctaatgatt taccacgcgt tttggcttga attgtcttac ctcgcgctgg gcgctgtgat
     2401 cgatcatttt tcggtaaagg catacctaca atttgaaaat caattaatcg ttcatctaca
     2461 tcaacatgcg tcactttaac cttaactgtg tcaccaattc taaatacttt agcttgacgc
     2521 tcaccaatta atgccatttg acgctcttca aaacggtaat aatcatcagt catattcgca
     2581 atatgaacca taccttctat cgtatttggc aattcaatga acataccgaa gttagctact
     2641 gagctgacaa taccttcaaa ttcatcacca atatgttgaa tcatatattc tgcttttttc
     2701 aattcatcag tatcacgttc tgcctcaata gcacgacgtt cacgtttaga agtatgttca
     2761 gctaactcag gcaatttgtc ttcccaacgc ttcacttctt tgttatccat tgatttctca
     2821 attaaatact tacggattaa acgatgaact gttaaatcag gataacgtct aattggtgat
     2881 gtaaaatgcg tataatattc agctgataag ccaaaatgtc ccaagttcac atcatcataa
     2941 tgcgcttgtt gcattgaacg caacatcatt gttgaaatga ccatttgttc aggtcgacct
     3001 tctacttctt cttgaacctt ttgaagtgtt gttggatgaa tatcttcgcc agtaccctta
     3061 atcatgatgc caaagtttgt aataaaatca aagaattgtc ttaagcgatc tgatttaggt
     3121 tgctcatgca ctcggtaaat aaaaggtaca tctaacttac taaaatgttc agcaactgtt
     3181 tcatttgcaa ttaacataaa tgattcaatt agacgttcac cctcgccacg ttgtcttaat
     3241 tgaacatctg ttggtatacc gtcttcgtta actaatactt ttgcttcatt aatatcaaaa
     3301 tcgatttcac cacgtcgttt tctcatttga atcaaacgat tagataaatc ttgtgctaaa
     3361 tctaacatag gcgtaatttc tttatattgt tcgcgaatgt taggatcctt ttcagtaata
     3421 atctgattta ccgcatcata cgtcattcga taatcagaat gtataacact atcaaaaatt
     3481 tcatgtttaa caacgcgacc actagcatcg atttccatgc gacagcttag agttaaacga
     3541 tcaacattag gattcaatga acaaatacca ttacttaatc gatgtggaat cattggaatt
     3601 acacggtcaa caagatatac acttgtcgct ctatcatatg cctctttatc caatgcagaa
     3661 ccttctgtta cataatagct gacatcagca atacttacag ttaattgcgt attaccgttc
     3721 gctaactttt taacactaat tgcgtcatct aagtctttag catcagcacc atcaattgtg
     3781 attgtcaatt catcacgtaa atcatgacgg cctttaattt cagtattttc aatatgatca
     3841 ggtactgctt cagcttcttg taacacttca tcaggaaatt caatttctat gccatgttga
     3901 tagataatag ataaaatatc tacgccagga tcatttttat gtcctaaaat agcagaaata
     3961 tgtccttctg gattatctga accatcagca tatttagtaa tttgaacaag taccttatgc
     4021 ccatctactg cacctaaact ttgtccttta ggaatgaaaa tatcttgcat aatacgttta
     4081 tcatctggaa taacaaatcc gaaatgtcta gcttcactat acgtaccaac aacttgagtt
     4141 actgaatgct tctcaatcga cttaacttcc ccttcaattt tacctttatg ttcaccttta
     4201 gattgatgta tttctacaat tacagtatct ccatctaagg cacgattaat ttttgttggg
     4261 ggaataaata tatcttccat atcctcatct tcaggtctta agaatgcaaa gcctttttta
     4321 ttttgactta acgttccctt tatcaatttt gaatgacctt ttgaactatg ctttttttga
     4381 tatctgtctg tttttgtacg ttcaattaat cctgattgtt ctaattctac gagcacctta
     4441 attaaatctc taaatgagtc ggcactactt aaacctaatg catcttgaaa atctgacact
     4501 gacataggtt catattcagg ttgattaata atctcttcta ttgattgctt taaattcatt
     4561 atgcccctcc tttctaatgt taaaatctct ttttattctg accagtctaa tgactctaaa
     4621 aattgataaa tatcttcaaa tacttgttct ttttctttat caatcgtaat aacatgtcct
     4681 gattgactat accacttgat gtctttgtca tcagaatcta catggtcata tatataattt
     4741 gcggattgtg gatcaatcat attgtcgttt tctgcttgaa tcactaaaat tgggtcaaat
     4801 acctcatcaa cttgatcttt aatcgtatct aatgcttcac ttagttcttt taaagtttca
     4861 gttggtttaa aatgatccat ttcattatca atagtctctt gatttttacc ttcatacttt
     4921 ttaaaattac gtgcatactc taaaaagcct tcataaatgg caccttcagt tttgccaccc
     4981 attggtgcac acatcgttac aatacccttt acatctctat ttaagcttaa ttttaaagca
     5041 aaatccccac ctaatgatag accagcaaca acaatttcat cataaccttg ttcaacaaga
     5101 taatcataac catctaacgc atctttaaac caaacgaaag gactagattt cagtatttcc
     5161 tctggtggtg ccgcgtgacc ttcatattgt ggtgcatacg aagtatatcc ctttttttgt
     5221 aaaaatcgac ctaattgacg tacatcagac gaattgcctg taaatccatg taataataac
     5281 acggcacgtt tgccttcttc aaaaaagaaa ggttttggta attttatctg cataaatcta
     5341 acgctccctt tgcatactta actaggctat atattttaca tagcttattg ttaaactatt
     5401 acattcttaa ttataaataa aaaagcccga catttacatc gccggacctt acataccaag
     5461 ataacttatg caaatcataa gtacaaaaaa taatattgat aaaataattg ttaatctatt
     5521 taagaataaa tcgacgccac gttgtttttg tttaccgaat aactgctcag caccaccact
     5581 gatggcacct gaaagtccac tgcttttacc ttcttggagt agtacaacag ttattaatgc
     5641 aatacaatca atgattaata atacgattaa aaatgtatgc ataaattgtc ctccgttcct
     5701 tatatacgac aagtagtata tcatattttt attgttaaaa tcaattttct agcgattaaa
     5761 tgtactttta cgtttgatag aaacaacaag catataaatc gctaatgcaa ccgaacctat
     5821 aataacgatt aaaattggca atgttaagta cgcaccgtgt gcagctttta ttgcttgaat
     5881 tgtgattgat aaattaatca acgtaaacaa actatttgat acataaactg caaacataaa
     5941 ccaccacaca taagtatgtc gattccaaaa agtgatgaag cctgctagat aagccaaaat
     6001 atacataata ccagtgaatc taaagctgtt taaaatgtaa tcaatcgctt tatctgaagt
     6061 gttttgattc gttaaattaa gtaatgcttt ggcagtcgca ttatccaacg aaacaaagta
     6121 atgaataatc aataatattg agaatacgat tgatgcagtt gcaataaagc gtcctgtttt
     6181 aatttcttta ttcgttctat cagccatttg tttactcctc atatatttaa atacgttcta
     6241 taaaaccttt atttaatttt acc
//
LOCUS       DAMZLE010000126         6257 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMZLE010000126 DAMZLE010000000
VERSION     DAMZLE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.127"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..841
                     /locus_tag="POB92_002193"
     CDS             <1..841
                     /locus_tag="POB92_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDI6579662.1"
                     /translation="LTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNK
                     MLNEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSNQ
                     TFKIFLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIR
                     SQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGI
                     KMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRY
                     ITN"
     gene            1147..1905
                     /locus_tag="POB92_002194"
     CDS             1147..1905
                     /locus_tag="POB92_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6579663.1"
                     /translation="MIHSKRLRLWLYLVLLAVFISACGMKEDKQIKENFNKTLSLYPT
                     KNLDDFYDKEGFRDQEFEKGDKGTWIVDSEMVVELKDKKMESRSMVLYINRNTRTTKG
                     NFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFVQYGDF
                     KDINDYKDGDISYNPNVPSYSAEYQLSNNDYNVKQLRKRYDIPTKKAPKLLIKGDGDL
                     KGSSIGHKNLEFTFVENKEENIYFTDSINFKPTK"
     gene            2017..2838
                     /locus_tag="POB92_002195"
     CDS             2017..2838
                     /locus_tag="POB92_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6579664.1"
                     /translation="MMHSKKLTLGICLVLLIILIVGYVIMTKANGQNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSGMNIQLKGGALKSREMVLYINRNT
                     RTTKGYFIVGEITKDKKGYTHDKDKKGYTHDKDKKYPVKMEHNKIIPTKPIKDEKLKK
                     EIENFKFFVQYGNFKDFKDYKNGDISYNPNVPSYSAKYQLSNDDYNIQQLRKRYDIPT
                     KKAPELLLKGDGDLKGSSIGSKDLEFTFVQNKRENIYFTDSVEFTPSEDTSYESN"
     gene            2952..3767
                     /locus_tag="POB92_002196"
     CDS             2952..3767
                     /locus_tag="POB92_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6579665.1"
                     /translation="MIHSKRLKLCLCLIILSVFIGACGMKKEESSKDKQIKENFNKIL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMIIETNNSNMESRGMVLYINRNT
                     RTTKGNFVVREITEDSKGYSHSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYNIPTNKAPKLLLK
                     GDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTPSEDTSYESNGISNKSW"
     gene            complement(3750..3872)
                     /locus_tag="POB92_002197"
     CDS             complement(3750..3872)
                     /locus_tag="POB92_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579666.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            4307..4498
                     /locus_tag="POB92_002198"
     CDS             4307..4498
                     /locus_tag="POB92_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579667.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(4637..4822)
                     /locus_tag="POB92_002199"
     CDS             complement(4637..4822)
                     /locus_tag="POB92_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579668.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(4758..5039)
                     /locus_tag="POB92_002200"
     CDS             complement(4758..5039)
                     /locus_tag="POB92_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6579669.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            5328..6023
                     /locus_tag="POB92_002201"
     CDS             5328..6023
                     /locus_tag="POB92_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6579670.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTVQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPSDRKKLDPQDIAEAVLYALTQP
                     SHVNVNEITVRPV"
BASE COUNT         2476 a          773 c         1098 g         1910 t
ORIGIN      
        1 attgacggaa aatgaatcta aaaatctggt tttcttttca agacaaatag cacctggatt
       61 gaaaagaatc gatagtttag ttttagaaga acttttaaaa aatgaattaa catatgatga
      121 attaaaaaat aaaatgttga acgaagttaa ggatataaca gaagatgata tagatacttc
      181 attaagaatt ttagattttt cattttacaa cgcaggaatt gaaaaaatct atggaagtcc
      241 cattatagaa tgtaatgaaa gaatgataag actaagcgat gcatttacta atgctctaag
      301 taatcaaaca tttaaaatat ttttagaaga tcttattgaa ttatcaaagt ataacaatga
      361 aaaataccaa aaaggtaaaa atggactgat tttatataat aaatattcaa gagaagattt
      421 tagcaaaata tttaattgga ataaaaatgg ttcgtctgta atcatgggat acatgataag
      481 aagccaagaa atgccaatat ttatcactta tgataagcat gaggatataa gtgatagcac
      541 aaaatatgaa gatgaatttt taagtcaaga tgaattaaaa tggtttacta aatctaatag
      601 aacgctaaaa tcaaaagaag ttcagaaaat tttgtcccat agagcaaaag gaattaaaat
      661 gtatattttt gtacagaaaa aagatgatga tggtatatat ttttattatt taggaactgc
      721 tggatatata gaaggttcag agaagcaaga taaaatgccg aatggttcaa acgtagtaac
      781 gatggattta gcactcgata aagctgtaag agatgatata tatcgctata taacaaatta
      841 atgtaaaccg aaaaatgaat gttagtaaag atgtatttaa acatcaattt taataagtaa
      901 agacattgag tttcagtgtt attattttaa atataagcgc aatttatctt aattaattag
      961 ttggttaggc caataaaaat tataaagata tttgcgcttt ttaaataatt tgtgatgttg
     1021 atatgtgaaa aatacaacta acaattaaaa gccaagagga tttaatcata aaattccctc
     1081 tagtattatt aatattggaa tttgaactta actttgcatc aaaaataaaa atgggggtgt
     1141 gaaatgatga ttcattcaaa aaggttgagg ctctggctgt atttagtatt attagctgtt
     1201 tttattagcg cgtgtggtat gaaagaagat aaacaaatta aagaaaattt caacaaaaca
     1261 ttgagtttat atccaaccaa gaatctcgat gatttttatg ataaagaagg atttcgagat
     1321 caagagtttg aaaaaggaga taaagggact tggattgttg attctgagat ggtagttgag
     1381 ctgaaggata aaaaaatgga atctagaagt atggtgctct atataaatcg taatactaga
     1441 acaacgaaag gtaattttat tgttagagaa ttgtgggaag atagtaaagg gtatgcacaa
     1501 agtaaagata caaaatatcc agtgaaaatg gaacataatc gaattatccc aaccaaaccg
     1561 atagctgatg ataaattaag aaaagaaatc gaaaacttta agttttttgt acaatacggt
     1621 gactttaaag atattaatga ttataaagat ggtgacatat catataatcc caatgtacct
     1681 agttattcgg cggaatatca attgagtaac aatgactaca atgtgaagca acttagaaag
     1741 aggtatgata taccaactaa gaaagcacct aaattattaa taaaaggcga tggtgattta
     1801 aaaggatcat ctataggaca caaaaattta gagtttacct ttgtagaaaa taaagaagaa
     1861 aacatctatt ttacggatag tattaatttc aaacctacta aatagggtta atagaagttt
     1921 ggaatgaaaa tttcataaag tgttcaaaat gatttttcgt tttagttgtg taaattaaag
     1981 attgagtcag tattaaaaat ataaggtgga gaaatgatga tgcattcaaa gaaattaacg
     2041 ctcggtatat gcttggtatt actcattata ttgattgtag gttatgtcat tatgacaaaa
     2101 gcaaatggtc aaaacgccca aattaaagac acatttaatc aaacattaaa attatatcca
     2161 accaaaaatc tcgatgattt ttacgataaa gaaggctttc gagatcaaga atttaaaaag
     2221 ggtgataaag gtacttggat agttaattct ggaatgaaca tccaattaaa aggaggagcc
     2281 ttaaaatcca gagagatggt actttacatc aatcgtaaca ctagaacaac gaaaggatat
     2341 tttatagtag gtgagataac taaagataaa aaaggatata cacatgataa agataaaaaa
     2401 ggatatacac atgataaaga taaaaaatac cctgtcaaga tggaacataa taaaatcata
     2461 ccaacaaaac ctataaaaga cgagaagtta aaaaaagaaa ttgaaaactt caagtttttt
     2521 gtacaatatg gcaactttaa agattttaaa gattataaaa atggggatat ttcatacaat
     2581 ccaaacgtac caagttattc ggcaaaatat caattaagta atgatgatta taatatccaa
     2641 caattaagaa aaagatatga tataccgact aaaaaagcac cagaactatt gttgaaaggt
     2701 gatggcgatt taaaaggttc atccataggc tctaaagact tagaatttac ctttgtacaa
     2761 aataaaagag agaatatcta ttttacagat tcggttgaat ttactccaag cgaggataca
     2821 agttatgagt caaactaaat agggttaata gaagtttgga ttgaaaattt cataaagtgt
     2881 taaaaaattt ttcgctttaa atgtataaat taaagattga gtcagtatta aaaatataag
     2941 gtggagaaat gatgattcat tcaaaaaggt taaaattgtg tctatgcctg ataatactat
     3001 ctgtttttat aggcgcttgc ggaatgaaaa aagaagaaag tagtaaagat aaacaaatta
     3061 aagaaaattt caacaaaata ttgagtttat atccaaccaa aaatcttgaa gacttctatg
     3121 ataaggaagg ctttcgtgac gaagagtttg aaaaaggtga caaaggcact tggataattc
     3181 actctaaaat gattattgag acaaataata gcaatatgga atctagagga atggtgctct
     3241 atataaatcg taatactaga acaacgaaag gtaattttgt tgttagagaa ataaccgagg
     3301 atagtaaagg gtattcacat agtaaagata caaaatatcc agtgaaaatg gaacataata
     3361 gaatcattcc aaccaaaccg atagctgatg ataaattaag aaaagaaatc gaaaacttta
     3421 aattctttgt acaatacggt gactttaaag atattaatga ttataaagat ggtgacattt
     3481 catataatcc gaatgtgcca agttattcag caaagtatca attaagtaat gatgactata
     3541 atgtgaagca acttcgaaaa agatataata ttccaaccaa caaagctccg aaactattat
     3601 tgaaaggtga tggtgattta aaaggctcat ctgtagggtc aaaaaatcta gaatttacct
     3661 ttgtagaaaa taaagaagaa aacatttatt ttacagattc tgtacaatat actccaagtg
     3721 aggatacaag ttatgagtca aacggaatat caaataaatc ctggtaatat aacaagtaac
     3781 tctgaagaaa caagttcgat atctaaagtg agctgtgaaa tataggtagc catttcaaaa
     3841 aatttaaagg tgaatttgat aatgtagctc aaggagattg gggtaaaaag gcgaagaatg
     3901 aactggatga tattagtaag aaattaaaaa atattcaaag aacggaagtt taatattcta
     3961 gccgattcct gaaactaaga cagcatgcgt tcattcatgc cattattaat ataagcaccg
     4021 caacaaaaaa gcttctaatg tgatatcgga acctcggatt ccatatcatg ttagaagctt
     4081 ttaatgtcta aagaacatct acattttatc atattttctt acttattaaa cttttatata
     4141 attaaatatt tcttaatttt ccaaaatagt gataaatttg tgaaatacat cacaaaaccc
     4201 tttatttatt tggaaattca tgtaatatta gtcttgtaag aagttaataa atagagagag
     4261 acgagagagt ttatataaat aatatataaa cattggagtg atgattatga gaaaagagat
     4321 tgaagcgctt attttctcag acgtaagtag ctatgatatt tacgtgaaca ctggtgtaaa
     4381 tcaaggatta attggtgaca tcaaagatgg ttacctaact attgattcta tgccttacat
     4441 tgatgctgag cgtttgtatc actttgctat ggaacgtaaa tcgttagtca ctaactagtt
     4501 cttattgcca atgattacta cccctagtct gcggcaattg aagtgtgatt gatgtaactt
     4561 gccctcgttg gtgagcaatt gagggcagac ccctttaatt aagtaaaccc taactcccca
     4621 caaatctgga acgatactaa aagccacgtc ctatattgga tgtggcttta gtcatgctta
     4681 tattattttt aaaacgatta cctacaagat ttacatataa aattctatca tgtctgctat
     4741 atataaacga aatgatatta gaatccttca ttttcaactt gtttaaaatt acgtttaatg
     4801 ccgttataag aatatacaac attatataat tttacttggg cgtcatggac acttgagaag
     4861 ggcattctta catcgtccat atttttaact tcgtgtcgaa atgctttttc agctttttgt
     4921 ctttctgcaa gcgacatact tagccattct tctttgctaa ataaatcttc gaatcgaaaa
     4981 tcaaatgcgg ttcttttact ttcagctctt aatgctaaaa cacgctcatg atatgtcatg
     5041 ttttattcct ccctctaatc attcaatcga atgataaatc acagtattaa ctttagtact
     5101 aatataatgt ttttacaata aagaagtgac gatttttcaa catttttaaa aataaaaaat
     5161 ataagcaacg attaattgta tatttcgaat ggcttggaat atactttatt cacgaaaatg
     5221 aagcgagtta ttatgaccgt tatatgaatt gtgatacgct ttttaaaagt aatatgtatt
     5281 tatgatttac cctgaaaaaa taaaaagaat caaaggagtc gatcattatg acagtattaa
     5341 cagataaaat agcagtagtt acaggtgcag gtagtggtat tggagaagca attgcaacat
     5401 tactacatga agaaggggcg aaagttgtct tagcaggtag aaataaagaa aaattacaaa
     5461 acgtagcgaa tcaattgtca caagatagtg tgaaggtagt gccaacagat gtaacgaata
     5521 aagaagaagt cgatgaattg ataaaaattg cacaacaaac attcggtggt ttggatattg
     5581 ttatcaatag tgcggggcaa atgttgtcgt ctaagattac tgattatcaa gtagatgagt
     5641 gggatagtat gattgatgtg aatatcaaag gcactttata tacggtacag gctgcattac
     5701 caactatgtt agaacaatca agtggccatc ttattaacat tgcatctatt tctggctttg
     5761 aagtaacgaa aagtagtacg atttatagtg cgacgaaagc agcagttcac actattactc
     5821 aaggattaga aaaagagttg gcaaagacag gtgttaaagt aacaagcatt tcaccaggaa
     5881 tggtagatac agcaataact gccgcatata atccaagtga tcgtaaaaag cttgatccac
     5941 aagatattgc agaagcggta ttgtatgcat taacacaacc gagtcacgtt aacgtgaatg
     6001 aaattacagt gcgtcctgta taaaaatgtg tttgaggtgt gggagataga catttaatgt
     6061 aatagctatc ggttttgtta atattagtaa ggcaataatg atataaaagc atgataggca
     6121 attattcctt gcacaatttt cactaactgg tgattttaac aatcgagtta atgaatgggg
     6181 cgatgttagt gttatggttt ataatctgta atgttcataa ttatagatta caaaaaattt
     6241 ttcattttcc accacat
//
LOCUS       DAMZLE010000127         6169 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMZLE010000127 DAMZLE010000000
VERSION     DAMZLE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.128"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            14..952
                     /gene="lukS-PV"
                     /locus_tag="POB92_002202"
     CDS             14..952
                     /gene="lukS-PV"
                     /locus_tag="POB92_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HDI6579671.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            948..1931
                     /gene="lukF-PV"
                     /locus_tag="POB92_002203"
     CDS             948..1931
                     /gene="lukF-PV"
                     /locus_tag="POB92_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HDI6579672.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2180..2365
                     /locus_tag="POB92_002204"
     CDS             2180..2365
                     /locus_tag="POB92_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579673.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2456..3361
                     /locus_tag="POB92_002205"
     CDS             2456..3361
                     /locus_tag="POB92_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDI6579674.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTKEAINKTQNSGYENEYFYIVANIPTLQEYRKYYEPLIKKNNLNFKK
                     GMKQARKGVGYKAAIEVHTTLFSRSSNFSKDKKLDDVIDLSESTKKLHLNFENTKIFL
                     QLAKSTISTNRVNYSDNESIRIEVE"
     gene            3419..4336
                     /locus_tag="POB92_002206"
     CDS             3419..4336
                     /locus_tag="POB92_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDI6579675.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGIEVYVHCDDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIQKNDKEFKE
                     GMERARKEVNYAADTDAVATLFSTKKNFTNDNTEDDVIELSDKLYNLKNKPDKSTITI
                     QIGKPTINTKKAFYDDNRPIEYGVHSKGE"
     gene            4393..5307
                     /locus_tag="POB92_002207"
     CDS             4393..5307
                     /locus_tag="POB92_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDI6579676.1"
                     /translation="MFKRIKLILIATIILSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVYVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAHKEELDNLTEVLK
                     EYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDKSFRD
                     GIRNSKKQLKDKSRPYVVTTLFSTKDNFTKDNTIDEMIDFSEVLKKKKNIPHDLNVSL
                     QISNKYINTTRPNYSKKDVIEVGVFNHE"
     regulatory      5521..5655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5717..>6169
                     /locus_tag="POB92_002208"
     CDS             5717..>6169
                     /locus_tag="POB92_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6579677.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSG"
BASE COUNT         2430 a          846 c         1065 g         1828 t
ORIGIN      
        1 ggaaatgatt tttatggtca aaaaaagact attagctgca acattgtcgt taggaataat
       61 cactcctatt gctacttcgt ttcatgaatc taaagctgat aacaatattg agaatattgg
      121 tgatggcgct gaggtagtca aaagaacaga agatacaagt agcgataagt ggggggtcac
      181 acaaaatatt cagtttgatt ttgttaaaga taaaaagtat aacaaagacg ctttgatttt
      241 aaaaatgcaa ggttttatca attcaaagac tacttattac aattacaaaa acacagatca
      301 tataaaagca atgaggtggc ctttccaata caatattggt ctcaaaacaa atgaccccaa
      361 tgtagattta ataaattatc tacctaaaaa taaaatagat tcagtaaatg ttagtcaaac
      421 attaggttat aacataggtg gtaattttaa tagtggtcca tcaacaggag gtaatggttc
      481 atttaattat tcaaaaacaa ttagttataa tcaacaaaac tatatcagtg aagtagaacg
      541 tcaaaattca aaaagtgttc aatggggaat aaaagctaat tcatttatca catcattagg
      601 taaaatgtct ggacatgatc caaatttatt tgttggatat aaaccatata gtcaaaatcc
      661 gagagactat tttgttccag acaatgaatt acccccatta gtacacagtg gtttcaatcc
      721 ttcatttatt gcaactgttt ctcatgaaaa aggctcagga gatacaagtg aatttgaaat
      781 aacgtatggc agaaatatgg atgttactca tgctactaga agaacaacac actatggcaa
      841 tagttattta gaaggatcta gaatacacaa cgcatttgta aacagaaatt acacagttaa
      901 atatgaagtg aactggaaaa ctcatgaaat taaagtgaaa ggacataatt gatatgaaaa
      961 aaatagtcaa atcatcagtt gttacatcaa ttgcattgct tttgctatcc aatacagttg
     1021 atgcagctca acatatcaca cctgtaagtg agaaaaaggt tgatgataaa attactttgt
     1081 acaaaacaac tgcaacatca gattccgata agttaaaaat ttctcagatt ttaactttta
     1141 attttattaa agataaaagt tatgataaag atacattaat actcaaagct gctggaaaca
     1201 tttattctgg ctatacaaag ccaaatccaa aagacactat tagttctcaa ttttattggg
     1261 gttctaagta caacatttca attaattcag attctaatga ctcagtaaac gttgtagatt
     1321 atgcacctaa aaatcaaaat gaagaatttc aagtacaaca aacggtaggt tattcttatg
     1381 gtggagatat taatatctct aacggcttat caggtggagg taatggttca aaatcttttt
     1441 cagagacaat taactataaa caagaaagct atagaactag cttagataaa agaactaatt
     1501 tcaaaaaaat tggttgggat gttgaagcac ataaaattat gaataatggt tggggaccat
     1561 atggcagaga tagttatcat tcaacttatg gtaatgaaat gtttttaggc tcaagacaaa
     1621 gcaacttaaa tgctggacaa aacttcttgg aatatcacaa aatgccagtg ttatccagag
     1681 gtaacttcaa tccagaattt attggtgtcc tatctcgaaa acaaaacgct gcaaaaaaat
     1741 cgaaaattac tgttacttat caaagagaaa tggatagata tacaaacttt tggaatcaac
     1801 ttcactggat aggtaataat tataaagatg aaaatagagc aactcataca tcaatttatg
     1861 aagttgattg ggaaaatcat acagttaaat taatagatac tcaatctaag gaaaaaaatc
     1921 ctatgagcta aacagataga taatcaaaaa atcttagata tgttaaaatt tacaaacact
     1981 ttctttctat attagggtaa ccacgtctta attgacgtgg ttattttttc agggcaaaaa
     2041 aagggcggat tatttaaata aggacaaaca cttgtggaaa atttaaaagg ttaaaaataa
     2101 taaagaactt ggtataacaa gggttttata catttgcgta caacgacgaa atgtcaattt
     2161 accatcacat tatgacgata tgtttatttt aaacacacaa gctcatgcgc gtcttgatca
     2221 aatggcacaa cagtttgaag ttgtttgtaa tggcttgaac gaaaatgaag gacaagcaat
     2281 tcaaacgatg gatcaatctg cctctctaat acggtcaaac ttaattcaag ttaaagaaca
     2341 attagaaaaa ctagctgtat actaattaat ttattaaatg ctacttgttt tcattgagaa
     2401 taagtagttt tttcaaacat aaaagtttta caaacacata aatgggtgat gagctatgtt
     2461 taaaagaact aaactaatct taatagcaac gattctacta tcaggatgtt caactaccaa
     2521 taacgaatcc aacaatgaaa caaaatctgt accagaagaa atggaagctt caaaatatgt
     2581 aggtcaaggc ttccaaccac ctgcagaaaa agatgcggtt gaatttgcga aaaagcataa
     2641 agataaaatt gctaaacgtg gcgaacaatt ttttatggat aactttggac taaaagttaa
     2701 agctacaaat gttgtaggta gtggcgacgg tgtagaagta ttcgtgcatt gtgatgacca
     2761 cgacatcgta tttaatgcaa gtattccatt tgacaaatca attattgaca gtgatagctc
     2821 attaagaagt gaggataaag gcgatgatat gagtacttta gttggtacag tgttgagtgg
     2881 ctttgaatat cgagcgcaaa aagaaaagta tgataattta tataaatttt tcaaagaaaa
     2941 tgaaaagaaa tatcaatata caggatttac taaagaggca attaacaaaa cacaaaatag
     3001 tggatatgaa aatgaatatt tttatatagt tgctaatata ccgacgctcc aagaatatag
     3061 gaaatattac gaacccctaa taaagaaaaa taatctgaat tttaaaaaag gtatgaaaca
     3121 agcaaggaaa ggagtaggct ataaagctgc aatagaagta catacaacat tgttttcgag
     3181 aagtagtaac ttttcaaagg acaaaaaatt agatgatgtt atagatttgt ctgaaagtac
     3241 gaaaaagtta caccttaatt ttgaaaatac gaaaatattt ttacaactag caaaatctac
     3301 tattagtact aatcgagtta attacagtga taatgagtct ataaggattg aggtcgaatg
     3361 acttgaaatc agctaatttc tctatattct aaacaaacac ataaatgggt gatgagctat
     3421 gtttaaaaga actaaactaa tcttaatagc aacattactg ctatcaggat gttcaactac
     3481 caataacgaa tccaacaatg aaacaaaatc agtaccagaa gaaatggaag cttcaaaata
     3541 tgttggacaa ggcttccaac cacctgcaga aaaagatgcg attgaatttg cgaagaagca
     3601 tcgtaaagaa tttgaaaaag taggtgaaca attctttaaa gacaactttg gactgaaggt
     3661 aaaggctacc aatgttgtag gtaaagatga tggtatagaa gtttatgtgc attgcgatga
     3721 tcatggcatt gtatttaatg caagtttgcc cttgtacaaa gatgccatcc atcaaaaagg
     3781 atcaatgcgt agtaatgaca acggtgatga tatgagtatg atggtgggta cagtgctgag
     3841 tggctttgaa tatcgagcgc aaaaagaaaa gtatgataac ttatataaat ttttcaaaga
     3901 aaatgaaaag aaatatcaat atacaggttt tactaaagaa gcaattaata agacacaaaa
     3961 cgttggatat aaaaatgaat atttttatat tacatactct tctagaagtt taaaagaata
     4021 tcgaaagtat tatgaacctt tgattcaaaa aaatgataag gaattcaaag aaggaatgga
     4081 acgagcaaga aaagaagtga attatgccgc tgatacagat gcagtagcga cactgtttag
     4141 tacaaagaaa aattttacta atgacaatac agaagatgat gtaattgaac taagtgataa
     4201 attatataat ttaaaaaata aaccagataa atctacaatt acaattcaaa taggtaagcc
     4261 tactattaat actaaaaaag ccttttatga tgataatcgt ccaatagaat atggggtgca
     4321 cagtaaaggt gaataaaatt ttaaaaaatt gcttttaata ttctaaacaa acacataaat
     4381 gggagatgag ctatgtttaa aagaattaaa ctaatcttaa tagcaacgat aatactatca
     4441 ggatgttcaa ctaccaataa cgaatctaat aaagaaacaa aatcagtacc agaagaaatg
     4501 gaagcttcaa aatatgtagg tcaaggcttc caaccacctg cagaaaaaga tgcgattgaa
     4561 tttgcgaaga agcataaaga taaaattgct aaacgtggcg aacaattttt tatggataac
     4621 ttcggtctaa aagttaaagc tacgaatgtt gtaggtagtg gcgacggcgt agaagtatac
     4681 gtgcattgtg atgaccacga tatcgtattt aatgcgagta ttccatttga caaatcaatt
     4741 attgacagtg atagctcatt aagaagtgag gataaaggcg atgatatgag tactttagtt
     4801 ggtacagtgt tgagtggctt tgaatatcga gcacataagg aagagttgga taatttaaca
     4861 gaagttttaa aagaatataa aagtaaatat aaatatactg gttatactga aaatgcaatt
     4921 atgaaaacgc agaatagtgg ttttagaaat gagtattatt atttgactgc aattccatat
     4981 actttagatg aatataaaaa gtattttcaa cccttaatca aagaggatga taagtctttt
     5041 agggacggta tcagaaactc aaaaaaacaa cttaaagata aatcacgtcc atatgttgtt
     5101 acaactttat ttagtactaa agataatttt actaaagata atacaataga cgagatgatt
     5161 gatttttctg aagtgctaaa aaagaaaaag aatattcctc atgatttgaa tgtttctctt
     5221 caaatttcta ataaatatat aaatacaaca agacctaatt atagtaagaa ggatgtaata
     5281 gaagtaggtg tatttaatca tgaataagct aacacgaatg attaaactaa aaacaataaa
     5341 aagataggtt caaaaaagcc atctcaaaaa ttaatcaaac aaacaacatt ccaaccaatg
     5401 ctcgcaaatc gcaaattcat tatacatcac caacttaatt aattttcatt ttaagcatag
     5461 ttattgaggt tttgtgatat atagtataaa attaatggga attaaattta ataatgtaaa
     5521 attcatcttc ggggtcgggt gtaattccca accggcagta aattaagcct gcgacctgct
     5581 agtatgtatc atattagtgg ctgatctagt gagattctag agccgacagt atagtctgga
     5641 tgggagaaga tggaggtttt ttgttgtgca ataatcctcc tattcttacg agatgaatgg
     5701 aaggagaaaa ttgaatatgc aacaaaataa acgtcttatc acaataagta tgttgagcgc
     5761 gattgcgttt gtgttaactt ttatcaagtt tcctatacca tttttgccac catacttaac
     5821 tttagatttt agtgatgtac cgtcactact agctacattt acgtttggac cagttgccgg
     5881 tatcgtagtt gcactggtta aaaatttatt gaactactta tttagtatgg gcgatccagt
     5941 tggaccattt gctaactttt tagcaggcgc aagtttctta ttaactgctt acgccatcta
     6001 taaaaataaa cgttcaacaa aatctttgat tactggatta atcattgcaa caatcgttat
     6061 gactatcgtg ttgagtattt tgaactattt cgttctatta cctttgtacg gtatgatatt
     6121 taacttagct gatatcgcaa ataatcttaa agtaatcatt gtttcagga
//
LOCUS       DAMZLE010000128         6121 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMZLE010000128 DAMZLE010000000
VERSION     DAMZLE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.129"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            122..664
                     /locus_tag="POB92_002209"
     CDS             122..664
                     /locus_tag="POB92_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDI6579678.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(1345..2262)
                     /locus_tag="POB92_002210"
     CDS             complement(1345..2262)
                     /locus_tag="POB92_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDI6579679.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGKYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            2352..2453
                     /locus_tag="POB92_002211"
     CDS             2352..2453
                     /locus_tag="POB92_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579680.1"
                     /translation="MVITDIDVYRVGRENIVIAVYNEHIKLLFILIS"
     gene            complement(2531..4036)
                     /locus_tag="POB92_002212"
     CDS             complement(2531..4036)
                     /locus_tag="POB92_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDI6579681.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(4377..4877)
                     /gene="queF"
                     /locus_tag="POB92_002213"
     CDS             complement(4377..4877)
                     /gene="queF"
                     /locus_tag="POB92_002213"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDI6579682.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(4897..5763)
                     /locus_tag="POB92_002214"
     CDS             complement(4897..5763)
                     /locus_tag="POB92_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6579683.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
BASE COUNT         2145 a          955 c          994 g         2027 t
ORIGIN      
        1 gcagtgtgaa tgatgaggtg agagtattca gtgtaaaaag caacaataga tgatattgtt
       61 ttgtatcaat tgcttttttg ctatactgaa tcaatactga tattttgagg agaagattaa
      121 aatgacccgt aaatcaatcg cgattgatat ggatgaagta ttggcagata cattaggaga
      181 aatcattgat gctgtcaatt ttagagcgga tttaggtatt aaaatggaag ctttgaatgg
      241 tcaaaaactt aaacatgtta ttcctgaaca tgatggatta attacagaag tattgagaga
      301 accaggcttc ttcagacatc ttaaagtgat gccgtatgca caagaagttg tgaaaaaatt
      361 aactgaacat tatgatgtat atattgctac agcagcaatg gatgtaccaa catcatttag
      421 tgataaatat gaatggttac tagagttctt tccattttta gatcctcagc attttgtttt
      481 ttgtggtaga aaaaacatcg ttaaagctga ttatttaata gatgacaatc ctagacagct
      541 tgaaattttt actggtacac cgattatgtt tacagcagtg cataatatta atgatgatcg
      601 atttgaacgc gtaaatagct ggaaagatgt agaacagtat tttttagata atattgagaa
      661 ataaaaatat atcacttgaa aaatttcatg tagtaaagat gatggatagg ctataaagta
      721 attgtgactt ggttgaactt ttatgtctta gacactacaa cactatattg gcagtagttg
      781 actgcggggc cccaacataa agaaattgga acgccaattt ctacagacaa tgcaagttgg
      841 cggggcccca acatagagaa attggaacgc caatttctac agacaatgca agttggggtg
      901 ggccccaaca taaagaaata ctttttcttt agaaattagt atttcttatg catgagtgta
      961 actcatgcat tcatattttt aagtacacat tagctgtgac taatgataaa gaatcgctac
     1021 ataatcaatc attagtcgtt ctttatcatt tccgtcccgc tctcaataaa tgttagtcta
     1081 tcttattatt ataaatcgga tgaatgtgtt aatctatggc agattacacg tcatccgatt
     1141 ttttatagaa tttgaaaaag acgcataaac cactatgatt taaaatacaa catcaatcat
     1201 tttagtggca tgcgccaaaa ttatatgtct gtttttgaaa cagggtaata gcttaaagct
     1261 aataaaaacg aatataaggt gcgttgaatc ttatgattac actccaaacc taatataata
     1321 tcgggttaag atcattccgg atgcttacaa atcattgaca gtaagtaact gaatggcatt
     1381 tggtataacc tcaatatcaa taggtgtttc taatgaaatt tcgccatcaa tatcaacttt
     1441 cattgctgga tctgttgtaa gtgaaatctt tttaccaggt atatgctcaa taccttgagt
     1501 aatttcattc caattcatgc tatcacgctt tttaaaaata tcatttaaaa tactgaaact
     1561 ttgttcatta aaaatgaaag tgttcagttc accatcttga ggagacaaat cagtcaatgg
     1621 tatacgacta ccaccaatga atggaccatt tgctgttagt atcatggtcg tttcgccaga
     1681 atatgtctta tcatctattg ataattgata attaaattgt gttggattta gcagtgtttt
     1741 gacagttgat ccaatataac tcaatttacc aaatatatct tttgaaccat cttgtacgtt
     1801 ttcagcgttt tgaacaatga gacctaagcc aacaaagttg agtgcatatt gattatttat
     1861 tttaattaca tcgtatttac caacttgtgc agaaatcatt tgttcactag cttgtttatg
     1921 attaggtgct atatttagcg tttttgtaaa atcattaaaa gtaccgcctg gtaaaatgcc
     1981 aatagggagt tgaaggtcat gtgtcataac accgtttata agttcgttaa ccgtgccatc
     2041 accgccaaga ataaataata tatctacatc ttttgcatag tttttagttt tgatttcttg
     2101 gcaatattta ataatgtcac cttcgttttc actcaattga atagaaagat gcttacaaat
     2161 tgaacttaat gctgttgtaa cttccccaat accttgatta atatttttta atccactgtg
     2221 ttcatggtaa aagaggacac catgtgtata tttattttcc atagtttagc ctactttcta
     2281 aaaattggtt cattaaatat atatacccac ttttaattgt taataccaaa aatatgtttt
     2341 aaatagagaa aatggtaata actgacattg atgtctatag agtgggacga gaaaatatag
     2401 ttatagctgt ctataacgag catattaagc ttttatttat actgatatct tgaatttaat
     2461 taatagaaac ctataaaaaa acagtaagcc atttaaatga cttactgttt tttgaattag
     2521 gccaacaata ttaacgtata cctttcatcg ctttgatgat taaaggtgag aatgctaata
     2581 caattgttgt aacaataatt gcaacaacac ctaggaaaat aaagtaattt gtttgaccta
     2641 gtggttctat taacttaact aaagtaccat tgattgcttg tgcagaagcg ttagttaagt
     2701 accaaatact catcatttgg gcattaaatg ctttaggtgc taacttaaca gcagcactat
     2761 tacccgttgg tgataagcat agctcaccga taacacaaat aatgtacgat aaaataaccc
     2821 agttaactga aaagtttgat gaacctgatg cataacctac aataccaatt agtatgtatg
     2881 acgcacctgc taagaacgta ccaattgcaa attttactgg caggctaggt tgtttagttc
     2941 caagcttttg ccataaaagt gaaataattg gagctagtaa taaaataaat aatgggttaa
     3001 ttgattggaa gatcgcttca ccaaagtttg ttttccaacc aaataagttt aatttcatat
     3061 ctgaatgttc aattccatat atgtttaata cattagaccc ttgttcttga atagcccaga
     3121 acaccattcc aagaataaat aatggaataa atgctttaac acgagaacgt tcagtatcag
     3181 tgacatcttt acttctaata attaaagtga agtaaatgat tggtaatgca atacctaata
     3241 ctaaaacagt attactaact aagttaaatg ataatgagtt agttaatgca ccaataacga
     3301 taattaatac aattgctaaa acaacacttc cgataataag accatacttt ttcttttcag
     3361 ctggtgtcaa tgggttagta ggtttcatac caacgctacc taagtttttg cggttgaaaa
     3421 gtacatacca tactaaacct aatgccatac caactgctgc aatcaagaat ccgccgtgga
     3481 agtttttaac attaacaaag tgttgcaaaa taataggtga taataatgca cccatattaa
     3541 ctgacatata gaaaataaca aaacctgcat ccatacgtct atcattttca ggatataaac
     3601 ggccaacgat atttgaaatg tttggcttca ttaaacctga accaataatg atgaagaaca
     3661 ttgatgtgaa taagccgatt aatgcaaatg gtaagcttaa acaaatatgt ccgataataa
     3721 taaagactgc acctaataaa gtagcgcctc tagtacctgt aattctgtca gcaatccatc
     3781 cgcctggtat tgatgtcata tagattaatg aaccataaac tgacataatt gacatagctg
     3841 ttgttttatc aattccaagg ccattatctg ttacggcaaa gtacatgtag aaaatgagta
     3901 gggcacgcat gccataataa ctaaaccttt cccagaactc tacaaagaag agtacgccta
     3961 gtcctcgagg atgcccgaaa aatcctgttt gaggtatgtc ttgaatttga tttccatggg
     4021 agttttgttg tgtcatgtat acatcccatc ctttcttccc ctaatacaat agtttaagta
     4081 aatttgattg tgccttatca actaaatatt ccaacaaatc acaaaaaaat aaaaatattg
     4141 agaatattct gttattaagc gcgcacaatt aattagagat aatataaaaa tatacttatt
     4201 gacgttaaag tgcaataggt ttctataaaa atgttgttta attcgtgtac taatattctt
     4261 ttggtgtact ttggtataaa atgttatttt aatgtaacct attatgtatg ctaaatgaaa
     4321 aacacaccaa taagttagga aaatcaactt attggtgtgt ttattaaatg atacatttaa
     4381 cgattatcta ttttttcggg atataaatca tgattcatca aacgatgctc agccattttt
     4441 tcatatttag aatttggacg tccatagttt gtataaggat caatagaaat tccaccacgt
     4501 ggtgtgaact tgccccagac ttcaatataa tgtgggtcca taagctctat caaatcattc
     4561 ataataatat tcatacaatc ttcgtgaaaa tcaccgtgat ttctgaaact aaataagtat
     4621 aatttcaaag attttgattc aaccatttta acatttggaa tatatgaaat atagatagtt
     4681 gcaaaatctg gttgcccagt aattggacat aatgatgtaa attctggaca gttgaatttt
     4741 acgaaatagt cacgaccttg atgcttatta tcaaacgatt ctaatacatc aggacgatag
     4801 tcaaaattgt aagtattgtc ttgatttcct aataaagtta tatcttgtaa ttcatcttgt
     4861 tgacggccat gtgccatata aagcgctcct ttaaatttat ttttttatta ttttggcgtc
     4921 tcggcgtgct ttttcaaaca tgtaataact tgcaccgata ataacgacgt aacctaatgt
     4981 tgcatagaaa tctggagatt ctccgaatag aataaatcca agtattgctg tgaaaattat
     5041 agatgcatac gtgaaaatag aaatatcttt tgctgctgca aaactatatg ctaatgtaac
     5101 accaatttga cccacagcgg cagctaagcc agcccctaat agataaagta tttgcatctg
     5161 actcattggt tcataggtat atgcagtgaa aggtattaaa acgatgacag aaaataagga
     5221 gaagtaaaat actatagtat atggtgcttc tcttgtacta agtgctcgaa cacatgtata
     5281 tgctgatgct gcaaaaatac ctgagaataa gccagctaat gatggaatca tagatgatga
     5341 aaattcaggt ttcactatta aaagcatacc taaaatagca attatcattg ctgtaatttg
     5401 atacttcctt accttttcat gtaagaaaac aatgcttaat aaaatcgtcc agaaaggatt
     5461 gagtttcatt aatgaatcgg catcactaag taccatatga tcaatggcat aaatatttaa
     5521 caatacacca ataagtccaa gtgttgatcg tgttattaat aagggttgac ttgaaagtct
     5581 gccaaacatt ggctgatggt atttatatat aaaaaataat ggaataaaca ttgctactaa
     5641 gtttcgtgct aatgattttt gaaaaacagg aaggtcacct gcaagtctga aaaacactga
     5701 cataaaactg aaaccaatag ccgaaattaa aatggcaatg atacctttta ctttaggatt
     5761 caattttatc gcctctttta tataaaatta acgtatttat attagcataa aacaacatgt
     5821 tgtgcataaa tagttgaaat ttactataaa aagactataa tagactgtac cgaacaaatg
     5881 ttctgtgttt atttgtcgga ataatagggc attacacttt tatgaatgtt tgtgttatta
     5941 cataaaacaa atatcaattc agtatcaagc taataagctt tttcttgatt tctgttgata
     6001 caattgagat tgacacagat ttaaaaaaat caagtgatat ctactaaaaa atttttttaa
     6061 atttgttcaa gtttttctaa tttagtattg gtgcctagtt ggaacgtttt acgaacattc
     6121 g
//
LOCUS       DAMZLE010000129         6047 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMZLE010000129 DAMZLE010000000
VERSION     DAMZLE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.130"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            46..1872
                     /locus_tag="POB92_002215"
     CDS             46..1872
                     /locus_tag="POB92_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDI6579684.1"
                     /translation="MGYRIETRTFEELEGKLKLPTFQRALVWSNNQKQEFFSTLKDGF
                     PFGSILLYEYENDNKYSLIDGLQRYSTMVDFMENPTNYIEFEDFTLKIAELYKGASES
                     TKAEVVNYAEDILLKIIEKYIDSTKENVKVNELAKKMVDKFPVLGETDIRDEITQIQS
                     DVITYFNNQLDISNVKIPCIFFEGEETELAEVFQRLNSGGKKLSKYQVFAAHWDRYEV
                     ELGDLKYSDEILKNVIERYERLNNDRGILIENFDPKEMETSRVINLSELCYGLGKVIS
                     DELQAFFKESTEDICNELGFQTMLIVFGIPTNKMNELPYKYQYMKNKNNIEILFEKIV
                     KIYKIINNKFKEAFSMVVSSREDKYETKSFTRLQIMSFFSSLWISSYSIELDENKNTF
                     SIKQTKSLKKNQQVILSNIIIYAIYDVIRKYWSGTGDRKLMDIYISQNNRYLKPLAKE
                     VFRNELLRWNEENINKTSINIQTDEKMIITFIANKFRSFYSSLNDNLDYEHIFSRKLY
                     KLHKGNAIIPAGSLGNIMLLDSGINRRKKEKFLYSAIDIDKASITGTIENQYVNNSFY
                     PDKRTIDTIEIDIIDEKYDSLIKMIKNRGDKLIEEVVRELYK"
     gene            complement(2410..2832)
                     /locus_tag="POB92_002216"
     CDS             complement(2410..2832)
                     /locus_tag="POB92_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579685.1"
                     /translation="MKSVNILLNLLPTELKNMLENKDMENILTYFMSNEISDEKLVSY
                     LSNLANQINTIEYHEMVASIYHFHFNYIDNAYDLAYYHYWQSLEISQFNNANLLYEFL
                     EILGEPDFNMISHENIQLVANKILERDPNNKLAIKYIN"
     gene            complement(2833..3420)
                     /locus_tag="POB92_002217"
                     /pseudo
     CDS             complement(2833..3420)
                     /locus_tag="POB92_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801062.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB-like protein"
     gene            complement(3686..3838)
                     /locus_tag="POB92_002218"
     CDS             complement(3686..3838)
                     /locus_tag="POB92_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579686.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFHNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            4201..4608
                     /locus_tag="POB92_002219"
     CDS             4201..4608
                     /locus_tag="POB92_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
                     /protein_id="HDI6579687.1"
                     /translation="MELSIFYNGQFFVALVEYKMENKSKFIQYTFGNEPDDIEVLDFI
                     HHQLMKMIDDVQTIVYTKNISRKVNPKKLQRQIAKEQKKPKYSTQAQIAIKKELELKK
                     KQKRKRYKEKRDAFQKRKREIKKVKAKEKHKGH"
     gene            4655..5137
                     /locus_tag="POB92_002220"
     CDS             4655..5137
                     /locus_tag="POB92_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDI6579688.1"
                     /translation="MILISSCLIGDNVRYDGGNQLNVRLKKLIDSGKAIHACPELLGG
                     LLIPREPAEIIGGDGFDVWNDAAKVVTISNKDVTDDYKHGAIVTLKILKKYQCDTVIL
                     KANSPSCGSQEIYDGNFTGNKKKGVGVATALLINEGIKVYDENTFFDQNMNETIVHEK
                     "
     gene            5337..6026
                     /locus_tag="POB92_002221"
     CDS             5337..6026
                     /locus_tag="POB92_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDI6579689.1"
                     /translation="MEKVAILVDGGYYRKISAKVYGKVTAKERADELYSYCNRHLKET
                     HFKEEIYNKLYRIFYYDCPPIDKIVYHPLLKKNVNFSNTDTKKWTEDFFKEMSKKRKV
                     ALRLGELSEYSVEYNLKYSITKKLLNGSIDLNDLKEKDFSLSLQQKGVDMKIGLDIAS
                     LSFKHQVDKIILIAGDSDFVPAAKLARTEGIDFVLDSLGADIRNNLSLHIDGRRTCDE
                     KFKFYLNQLFK"
BASE COUNT         2236 a          690 c         1016 g         2105 t
ORIGIN      
        1 tttatagcta tttaattcgt atttgagata aaaggagagg tttggatggg atatagaata
       61 gaaacaagaa cgtttgaaga gttagaagga aaattaaagt taccaacttt tcaaagagca
      121 ctagtttggt ctaataatca aaagcaagaa tttttctcaa cgctgaaaga tggttttcct
      181 tttggttcta ttctgttata cgaatatgaa aatgataata aatacagtct tattgatggc
      241 ctgcaaaggt attctacaat ggtagatttc atggagaatc caacaaacta tattgaattt
      301 gaagatttta cattgaaaat tgctgaattg tataaagggg catcagagag tacaaaagca
      361 gaagtagtta attatgccga agatattctg ctgaaaatta ttgaaaaata tattgattct
      421 acaaaagaaa atgtaaaagt aaatgaacta gctaaaaaaa tggtggataa atttccagta
      481 ttaggtgaaa cagatattag agatgaaatc actcaaatac agtcagatgt tattacttat
      541 tttaataatc aactagatat tagtaatgtc aagattccgt gtatcttctt tgaaggagaa
      601 gagactgaat tggcagaagt ttttcaaagg ttaaacagtg gaggtaaaaa gttatcgaag
      661 tatcaagttt ttgctgcaca ttgggacaga tatgaagttg aattgggaga tttaaagtat
      721 agtgatgaga tacttaaaaa tgtaatagaa aggtacgaaa gattaaataa tgatagaggt
      781 atcttaattg aaaattttga ccctaaagaa atggagacgt caagagtaat taatttatct
      841 gaattatgtt atggacttgg taaagtgatt tctgatgaat tacaagcatt ttttaaagag
      901 tcaacagaag atatatgcaa tgaattagga tttcaaacaa tgctaattgt atttggcatt
      961 cctacaaata agatgaatga gttaccatat aaatatcaat atatgaagaa taaaaataat
     1021 atagaaatct tgtttgaaaa aattgtgaag atatataaaa taataaataa caagtttaaa
     1081 gaagctttct ctatggtagt atcgtcaaga gaagataaat atgaaactaa atcttttact
     1141 agattacaaa taatgtcatt cttttcatct ttatggatct catcgtatag tattgaatta
     1201 gatgaaaata aaaacacatt ttctattaaa caaactaaat ctttgaaaaa aaatcaacaa
     1261 gtgattttgt ctaatatcat aatttatgca atttatgatg tgataagaaa gtactggtca
     1321 ggaacaggtg atcgaaaatt aatggatata tacatatctc aaaacaatag atatttaaag
     1381 ccgttagcaa aagaagtgtt tagaaatgag ctacttagat ggaatgaaga gaatataaat
     1441 aagacaagta ttaatattca aacggatgag aagatgatca ttacttttat tgctaataaa
     1501 tttagaagct tctattcatc tttgaatgat aatttagatt atgaacatat tttttcaaga
     1561 aaattataca agctgcataa aggtaatgca ataatccctg ctggatcttt aggtaacatc
     1621 atgttacttg actctggtat taatagaaga aaaaaagaaa aatttcttta ttcagcaata
     1681 gatattgata aggcatctat tacgggaact atagaaaatc agtatgttaa caacagtttt
     1741 tatccagata aaagaacaat agatactatt gaaatagata taattgatga aaaatatgat
     1801 agtttaataa aaatgatcaa aaatcgtggt gataaattaa tagaagaagt tgttagagaa
     1861 ttatataaat aagtttgata tatcttgctt tttattatca cgctagttga tggtatcttt
     1921 aaagtgtgta ttacctgatt gatatcaata ccaggtatcg aatgaatatt tttaaaataa
     1981 ttgagttgtt gtgctaattg aatcatggca tcatttaatt gtttgatacg atggatagat
     2041 tgttttaatt gtttaggcgg cctttgttgg tcgccatttt tatataataa tataaatgac
     2101 caaaccacag cacctattaa tttaagagtg aggtcatttt taaaggtaaa aaaggagtaa
     2161 ttttatatat taaaatgata tataaaacaa gtagtattag tatgttgtga attgttatag
     2221 aatttgactt gttagcaatt tcgtagttat tgtaatagca agtactgtat ggttttattt
     2281 tttaaaggta gtgaaataat attggatttt tgaagtgaaa tatttgtaga tggatacaac
     2341 gaagcatatt agaaatacaa aaggaacgat gaaattaaat gtccatcatt ccttatgcct
     2401 agtagttttt tagttaatat atttaattgc taacttatta ttagggtctc tttcaagaat
     2461 tttatttgcg accaattgaa tattctcatg tgaaatcata ttaaagtctg gttctccaag
     2521 gatttctaaa aattcgtata ataaattagc attattaaat tgcgaaatct ctaacgattg
     2581 ccaataatga tagtatgcta aatcataggc attatctatg taattgaaat gaaagtgata
     2641 gatgcttgcg accatttcat gatattcaat tgtatttatt tgattcgcaa gatttgataa
     2701 atatgatact aatttttcat ctgaaatttc gttcgacata aagtatgtta gaatattctc
     2761 catatcttta ttctctaaca tattttttaa ttcagttggt aataaattta atagaatgtt
     2821 aacgcttttc atttatttaa cccatccatt cgatatatat attgccattt tttcgtgtat
     2881 aagttatgac tacagttcta tcactcatga caagtgtttt ttggtatgcg gactttttgt
     2941 atcggctttg ctttccatac ctcataacat gcgacattac tttagaaaca tctttccaat
     3001 tgtttttagt aactttattc caattatgtt tactagcaag aatatgtttt cttttattag
     3061 ctcctagatt ggctacagaa ggtaaaaatt taccaaaact ctttgttttg atagcggttt
     3121 gagttgcttt ttttccatat gttttaattg catagtttat tccgtatcag ctactgcagc
     3181 taatattact aatggcaatg cagaagattt agcttcaatt gagttgaatt ttattatttc
     3241 gccagtttct ttgtcgatta atgtagcttc atattcttca ttttcaaatt gttctacgaa
     3301 aacgtcataa tccttattta ttagttttcc attttcatcg acgtatctgc ccttatcatc
     3361 catagtatta gtattcaaat catattgaat atttttatct aaatcaatat cttttttcgt
     3421 ttttccacta acgtcttttt caagctttat atttacaatt tttaagcaga agtatcccat
     3481 tatttcaagg tctgatttat ctataattaa atacgtacgt atggaatttt gatcgttcat
     3541 ttttattgct ttatgtttta ggaaatgtgc tattgaaggg tttaaagatg tttggaattt
     3601 cttggtttct tgacttatat agttatgaaa gagggcgttg tcctttatta aatttctgag
     3661 atcagaaatg ctataaataa agtgattaag attttttgtt ttcatgtaat ttaagaaatg
     3721 gatttgtttt ttgtgggtta tttttaattc tattatgaaa tggtttgaat tttgcagttg
     3781 caaatatgat agctgcttct tttgacaaag taatagaacc tttttcttct ggtaccatga
     3841 tattcactcc ttgtacaatt caatttaagt attataaaat aattttacta tacaattacc
     3901 tttgtgtaaa tcgtttctta ttgttaccaa attatttgtt taaaagcatt atgcaatgcc
     3961 aatcaacact aatggggtgt aagtatttcc ctgttaatat tttattagcg ttatttacat
     4021 ataccttctg taatgtgaat ttttaaaatt acagtttaca tgtaacgttg tgcaatatat
     4081 tctgtatata gaagtgtaaa aacacaattg ggttggtagt cccaatgcag ttttaaaact
     4141 gtcagtaacc ttcctcctcg ggtcgtccgt catttctaat aaaactagag gagggatagc
     4201 atggagctat ctatttttta caatgggcaa ttttttgtag cattggtaga atataaaatg
     4261 gaaaataaat ctaaatttat ccaatacaca tttgggaatg aacctgatga tatagaggta
     4321 ttggatttta ttcatcatca attaatgaaa atgattgatg atgtgcaaac tattgtttat
     4381 acgaaaaata tttctagaaa agtaaacccg aaaaaactac aacgacaaat tgctaaggag
     4441 caaaagaaac ctaaatattc tacccaagca caaatagcta ttaagaaaga attagaatta
     4501 aagaaaaagc aaaagcggaa gcgctataaa gaaaaacgtg atgcatttca aaaaagaaaa
     4561 agagaaatta aaaaggttaa agcaaaagag aagcataagg ggcattagcg atacgacaaa
     4621 taaattaaaa caaatagatt tataggtggt tgggatgatt ttaattagtt cgtgtttgat
     4681 aggcgataat gtaagatacg atggtggtaa tcaattgaat gttagattga aaaaattaat
     4741 agacagcgga aaagctattc acgcatgtcc agaattactt ggtggattat taattcctag
     4801 agaacctgca gaaattattg gtggcgatgg ttttgatgta tggaatgacg ctgcaaaggt
     4861 tgttactatt tcgaacaaag acgtaactga cgattataaa catggagcaa tagttacatt
     4921 aaaaattttg aaaaaatatc aatgtgatac agttatttta aaagcaaata gcccatcatg
     4981 tggatcacaa gagatatatg atgggaattt tacaggaaat aagaaaaagg gtgtaggtgt
     5041 ggcaactgct ttactcatta atgaaggtat aaaagtttat gatgaaaata cgttttttga
     5101 ccaaaacatg aatgaaacga tagtacatga aaagtaagta ttgaggatat acgtaataaa
     5161 ccaaacattt aagttgtaag atactttata aaatattttt taaaatcgtt gaaatgatat
     5221 tcaagttgtg ataatatatc ggtacagata attttgttaa cagatccatg agagtaggaa
     5281 ccccaccata cgggaatttc gaaatcatgg attttttgat ataaaggagt gatgtaatgg
     5341 aaaaagtagc cattttagta gatggtggat actatagaaa gataagcgca aaagtctacg
     5401 gaaaagttac agcgaaagaa agagctgacg agttatatag ttattgcaat agacacttaa
     5461 aagagacaca tttcaaagaa gaaatatata ataaattata cagaatattt tactatgatt
     5521 gtcctcctat tgataagata gtttatcacc ccttattaaa aaagaatgta aatttttcca
     5581 atactgatac gaaaaagtgg acagaagact ttttcaaaga aatgagcaaa aaaagaaagg
     5641 tagcattgag gttaggagag ttgagtgagt attcagttga atacaactta aaatactcta
     5701 ttacaaaaaa attattaaat ggaagtatcg atttaaatga tttgaaagaa aaagattttt
     5761 ccctatcatt acaacaaaaa ggtgttgata tgaaaatagg tttagacata gcttcattat
     5821 catttaaaca tcaagtagat aaaattattt taatagctgg tgatagcgat tttgtacctg
     5881 ctgctaaact agcaagaaca gaaggaattg attttgtatt agattcttta ggggcagata
     5941 ttagaaacaa cttgtcatta cacattgatg gtaggcgtac ttgtgatgaa aaatttaaat
     6001 tttatttaaa tcaactattt aaataataat ttttttaaaa cgcatct
//
LOCUS       DAMZLE010000130         6041 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMZLE010000130 DAMZLE010000000
VERSION     DAMZLE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.131"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            184..1122
                     /locus_tag="POB92_002222"
     CDS             184..1122
                     /locus_tag="POB92_002222"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDI6579690.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYNHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSHLDTISKKHFKNREKAQKILKPL"
     gene            complement(1357..3759)
                     /locus_tag="POB92_002223"
     CDS             complement(1357..3759)
                     /locus_tag="POB92_002223"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI6579691.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKDKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPDTTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMSVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3759..4817)
                     /gene="pheS"
                     /locus_tag="POB92_002224"
     CDS             complement(3759..4817)
                     /gene="pheS"
                     /locus_tag="POB92_002224"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI6579692.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5198..5938)
                     /locus_tag="POB92_002225"
     CDS             complement(5198..5938)
                     /locus_tag="POB92_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDI6579693.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1941 a         1168 c          854 g         2078 t
ORIGIN      
        1 tttatcatgt ttaaattgtg tcactataac attttcataa acattataca tgaccactat
       61 gtattttgta agtatccgca attaattctt tacaacatac ataaatgttt ctgacgttat
      121 tatcatttat gatatgatta tttttgctaa agataatgaa attttatgaa aggatttaca
      181 caaatggcga atatcgtttt taaattgtcg gataaagaca taacgacatt aatgtcacgc
      241 attacttttg atactgagaa tttacctcaa ggtatgaaag cacgtgcaaa gtatcaaaat
      301 acaactgtaa atatttacca atcaggtaaa gtcatgtttc aaggtaatca tgcagaagct
      361 gtgtctaaag aacttctacc gcagcattct caattgaata ctaataaaac taaaaagaaa
      421 aatatggcta attcttttct tgaacaaact ttaatgtatg atcaatttaa ctgtattggt
      481 agtgatgaag ctggaagtgg cgattacttt ggtccattga ctgtatgtgc tgctttcgtt
      541 accaaagagc atgtccctat tttaaaaact ttgggtgtag atgattcaaa aaaattaacc
      601 gataccaaaa ttgtagaact tgctgagcaa ttggttactt tcatcccaca ttcattatta
      661 acactacaca atgaaaaata taatattcag caagcaaaag gctggactca agtaaaaatg
      721 aaagctgttc ttcataatga agccatcaaa aatgtacttg aaaaaattga ttcgtctcaa
      781 ttagactata tcgtcattga tcagttcgca aaacgcgaag tgtataacca ttacgcatta
      841 tccgatatac cacttccaaa gaaaacaaaa tttgaaacaa aaggagaatc gaagtcatta
      901 gccattgcag tggcaagcat aatctctcga tacgcatttg ttacatatat ggatcaaata
      961 tcaaaaaata ttaatatgac gattcctaaa ggtgccggtg ctaaagtcga tgtaatcgcc
     1021 gccaaaatta taaaaaaata cggtctttca catttagata ccatttcaaa aaagcatttt
     1081 aaaaatcgtg aaaaagccca aaaaatttta aagcctcttt gatttttaat cattataaat
     1141 tggatcctaa taggaaatcc tattaggtaa gtcatactaa tttgctttgc tcataagtat
     1201 gactttatat gcagttgtgc gaagcatcat ctgtattcca ctgttcctct ttgacttttt
     1261 aaaccaatcg aatcctaatt ggtgaatcca aaaataaaac cccatgttag ggatatcaat
     1321 ctacttcaga tatccttata catggggttt aaatcattat ctaataacag caccttgttc
     1381 aattaatgct gcttcaattt ccgcttgtac ttttgaaacg cgctcatctg tcaatgtttc
     1441 ttctgtgtct aaataattta aacgtattgc aattgatttt ttaccttttt ctaaatgttc
     1501 gccctgatat acatcaaaga caagtgtatc ttttaatata ttgccaccgt gtgcatgaat
     1561 cgttgataat aaatcagctg ctggaatatt ttgatctact tctaatgcaa tgtcacgaga
     1621 catgcctggg aatctcggaa ttggctggta attaatgtaa cctaccgaca cagacattaa
     1681 tgcatcaaaa ttcaactcaa aaacatacgt acgttttaaa tcattatcag ctgctaatgt
     1741 tggatgtaat tcaccaataa aaccaacaac tttattttct aataagattt cagctgtacg
     1801 acctggatgt aatccatcaa tatcggcacg acgataacta aattcaaggt ttaacttttc
     1861 agatactcga tccacgacac cttttgctaa atagaaatca accgtttctt tcttaccttg
     1921 ccattgattg actacataat ctccagttaa aataccactt aaatattcaa cttgatctgg
     1981 tagttcacct tctccattag caaagaagac attgccgatt tcaaataatt ttacatcttt
     2041 atttttgcgt gccacattat atgatgctgc ttcgattaaa tgtggtaata aactttgacg
     2101 taatgacgca tgcgcttcac tcattggcat caataaatca attgtttgac gctgttgcat
     2161 cgaaaatgca gtagcatcct ctttagaaac taacgaatac gtaatagctt gatctaatcc
     2221 agcaccttct aacacttctt taaccattct cgttttatat tggcgatcag ttagctgacc
     2281 actagtaact ttatcgaaga caggtaacgt tgatggaata tcgtcgtatc catatatacg
     2341 tgcaacttct tcaattaaat cttctttaat tgtaatatct ttacgacgtg atggtactag
     2401 cactgtaata acatcatcat ttatttctgt atcaaaccct agttgattaa aaatagtaac
     2461 aatatcattt tgtgacaaat caaatccaat agtgcgatta attttatcag cagtgatgtc
     2521 gattggtgta ataaatgcac caagttctcc tgaagacact ctatctttta gcacttttcc
     2581 gtttgcataa gtttgtaata aataacatgc acggtcgact gcttcatcta caaattcagt
     2641 agctattcct ttttcaaaac gactagatga ttcactgcgt aaatttaaac gtcttgatgt
     2701 atgacgaatt gaaactggat caaaaatagc accttcaatc actatatttg atgtttgttc
     2761 tttaacttct gaaaaatcgc cacccataac accagctaat gcaattggag tttgtccatt
     2821 agtaatgaca atatcgctcg ttaataattc acgttctgta tcatctaatg ttgtcatttt
     2881 ttcgccttca ttagcttgac gaacaacaat ttgttgtgaa ccaatcgcat cttgatcaaa
     2941 catgtgcaat ggttgaccgt attctaataa cacataattt gaaatgtcaa caacattatt
     3001 aataggacgt atacccgctt ttattaagcg tgcttgcatc caaattggcg agggttcaat
     3061 tgtcacgtcg tgaacaacac gtgcactata atatggtact ttatcttcat tttctattgt
     3121 cacagtcagt tcatcatttg cagataactc aagctcattt gatgttgtgt ctggcttagt
     3181 catttttgta ttatacaatg ctgcaacttc ataagcagta cctatcatac ttaaagcatc
     3241 tgcacgattc ggcgttaaat caaattccat tacttgatca tctaaatata aagcttgtaa
     3301 ggcatctgtt cctggaactt gggattcact aaatacataa atgcctgatt caaaactttt
     3361 cggtatataa ttacttgaaa taccaatttc ttgtaacgaa caaatcatac cttctgaacg
     3421 ttcaccgcgt aatttggcac gcttaatttt aataccacca ggcaatcttc cacctacttt
     3481 agcaacaatg acatattgtc ctgcatcaac gttcggtgca ccacatacta tttgtacagg
     3541 ttcgtcttct ccgatatcaa cttggcaaac atttaattta tctgcatcag gatgtttatc
     3601 ttttgactta acgaagccga caactaaatt tttgatatct tttgtgtagt caattaaatc
     3661 atccacttca atacctgtgc gcgtaatacg ttctgccaaa ttacttacag aatcatcgat
     3721 tgttacatat tctttcaacc attcatttga tatcaacatt acatgtcacc tctatcttct
     3781 accgctttaa attgatctaa aaatctcaca tcattagtat agaaatgacg aatatcttct
     3841 ataccatatt tcaacattgc aatacggtct ggtcccatac caaatgcaaa tccagagtac
     3901 tctgaagaat caaaaccagc catttctaat acattaggat gtaccattcc agcacctaaa
     3961 atttcaatcc atcctgtgtg tttacacaca ttacaacctt ttcctttaca tttaaaacat
     4021 gacacatcaa cttctacaga aggttcagtg aatgggaagt aacttggacg taaacgaatt
     4081 tcacgatcag caccaaataa tttcttagct aacaattcta aagtaccttt caaatcactc
     4141 attttaacgt ttttatcaac aactaatcct tcgatttgtg taaattgatg actatgtgtc
     4201 gcatcatcag agtcacgacg atacacttta ccagggcaaa taattttaac tggaccttga
     4261 ccatgacgtg attccatcgt acgtgcctgc actggtgatg tatgcgtacg taataaaatt
     4321 tcatccgtaa tatagaaact atcttgcata tcacgtgcag ggtgtgattt aggtaaattc
     4381 agcatttcga agttataatg atcttgttca acttcatatc cattcacaat ttcataacct
     4441 aaacctaaga ataagtcttc aatttcttct attgtacgtg ttaatggatg ctttgaaccg
     4501 atttcaatat gacgacctgg taatgataca tcaattgttt cttcagccaa ttgcttattt
     4561 aatttttctt taactaacat ctgttgtctt tcatctaatt cattttgaat tgtttgacgc
     4621 aattcattca ctttttgacc aaatgcaggt ttctcttcat tcggcaaatc cttcatcaat
     4681 ttcattagtc cgctaactga ccctttttta cctaagtatt tcactttaac ttcttgcagt
     4741 gcacgttcat catttgcttc attaatatct acaagcgctt gttgttttaa ctctgacatt
     4801 gtttgttgtt cagacatatg tcactgacct ccctattcga actattcatt ttatatttat
     4861 catatttgtt gtttaactaa aaaaagactt tcatcctctt aaaaaataag ggacgaaagt
     4921 catttccgtg gtaccaccca aatttatgcc taagcataca cttgtttaat attgataacg
     4981 gattgtcttt ccgacttaaa tctctttaag taaccaaaaa ggtgaaaaga ctttaaataa
     5041 ttggaataac ttgcagtcac gattattctc cctaaaacaa ttatttatat aaatgtccat
     5101 gcctttttat tggtttatat tcaattgtgt atgcaatcac gtagaaatgt tctgttaaac
     5161 aattaattat aattatatcg gaaaactttc aacacggtca acctttcaaa tgataaagta
     5221 aaatactacc tgcaatcgct acatttaaac tttcagcttt accataaata ggtatgatta
     5281 aattttgtgt agtatgtgct aataattcag gattaacacc ttctccctca ttacctaata
     5341 gtaatgcaaa agaatcactt gaagtaactt ctttgtatgc cactgcgttt tcaagtgctg
     5401 taccataaac aggaccatta aattgagtaa taaacgtatc gagatcttgt gtcataactg
     5461 gcaaatggaa aacactacct tgactcgctc gcaacacttt atcttgataa ggatctgtcg
     5521 tacccttctc cattattaca gcatccattc cagcagcatc cgcagttcta attaatgtgc
     5581 ctaaatttcc aggatcttga acacgatcga tgagcaatac ttgttgtgct ttagaaatat
     5641 catagtgcgg cttctcaatg attgcgaaaa acccttgtgg tgtcactgta cctgataaag
     5701 attcagcaac tttcatgttt atttcaaaaa cttcttgcgc gtatgcgata atttgctgat
     5761 ctaatcttgc cggttcaatt gcaaataatt gtgtaattac aattccactt tgataagctt
     5821 cttcaattaa atgcacacct tcaattaaag ctaatccagt tttatccctc tcacgtttct
     5881 tttttagctt gttcgcttgt ttaattctat tattttgtgc agaagtaatt tgttccatag
     5941 atagctcctc gctttatttt taaaaataaa aatattaatc attaataaga tgaaaacatt
     6001 tgattgtata gttaatatta attaatcgat tttatcactc a
//
LOCUS       DAMZLE010000131         6023 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMZLE010000131 DAMZLE010000000
VERSION     DAMZLE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.132"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            63..680
                     /gene="cadD"
                     /locus_tag="POB92_002226"
     CDS             63..680
                     /gene="cadD"
                     /locus_tag="POB92_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDI6579694.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRNIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            699..1046
                     /locus_tag="POB92_002227"
     CDS             699..1046
                     /locus_tag="POB92_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6579695.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNQEGF"
     gene            1215..1307
                     /locus_tag="POB92_002228"
     CDS             1215..1307
                     /locus_tag="POB92_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6579696.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            1519..2493
                     /locus_tag="POB92_002229"
     CDS             1519..2493
                     /locus_tag="POB92_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDI6579697.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFN
                     SSSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE
                     MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLIQSVPSEIKENS
                     GWIRVQDCGKNVKKIKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIE
                     DIENYLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYE
                     EDLKLLQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
     gene            2588..3196
                     /locus_tag="POB92_002230"
     CDS             2588..3196
                     /locus_tag="POB92_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6579698.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSITNRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEVINTVNYLKDKEVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNENKRRQAQGIQVAKEKGVYKGRPQLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKTISKIAKEVNITRQTIYRIKHDSGLS"
     gene            complement(3940..4443)
                     /locus_tag="POB92_002231"
     CDS             complement(3940..4443)
                     /locus_tag="POB92_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579699.1"
                     /translation="MNKKQGLIAIVVGCSVILGACQGVHDDPLANKKYKVEDTDYSIS
                     FNGKDRAMMSWTEGAPDNMIKDSTPFNYEIKKDAGKKFLVLERDSSEFPDGYKDELHF
                     YDDRSIDYDHSKYYEVYEIKEKNKDDITLTEKDLQQSINDREIDKKLFKHPDKSEDGR
                     DLALTKQ"
     gene            complement(4460..4858)
                     /gene="tnpA"
                     /locus_tag="POB92_002232"
     CDS             complement(4460..4858)
                     /gene="tnpA"
                     /locus_tag="POB92_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDI6579700.1"
                     /translation="MFYNKRTQSLYDLNYYLVIATNQKRSYISEKQSTLLKRHFLKVA
                     KTYSVHLLYWEYHDFYIEISFSVDKGQTSVSDFVAVFKSTSSFNIKKYFPEILDERIE
                     SKPFWNKGYFLKTLGSVSEKEKDEWLKKLK"
     gene            complement(4956..5177)
                     /locus_tag="POB92_002233"
     CDS             complement(4956..5177)
                     /locus_tag="POB92_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579701.1"
                     /translation="MTKNTYNESMYQDYIGQQVIFYLFTEKRIIATIKEERRFELLIE
                     RIVKYKNSGKIEQKKTILPKSAIIFEDLK"
     gene            complement(5256..5483)
                     /locus_tag="POB92_002234"
                     /pseudo
     CDS             complement(5256..5483)
                     /locus_tag="POB92_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5809..5910)
                     /locus_tag="POB92_002235"
     CDS             complement(5809..5910)
                     /locus_tag="POB92_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6579702.1"
                     /translation="MVEALVHITTTVISGCIVALFTHWLRTRNNKKK"
BASE COUNT         2113 a          808 c          914 g         2188 t
ORIGIN      
        1 ctatgattat ttaaaaatat aagttataat acattcaaaa atatttttga atgaggtgta
       61 ttatgattca aacggttgtt gctgctgctg ttctttatat tgctacagca gttgatttat
      121 tagtaatttt attaatattt tttgctagag caaagactag aaaagaatat agaaatattt
      181 atgttggtca atatttaggg tctattattt taatattagt tagtttgttt ttagcttttg
      241 tattaaatta tgttccagaa aagtggatat taggtttatt gggtttaata ccaatttatc
      301 ttggaattaa agtggctatt tatgatgatt gtgaaggaga aaagagagct aaaaaagaat
      361 tgaatgaaaa aggattgtct aaattagttg gtacggttgc aattgttacg atagcaagtt
      421 gtggcgccga taatattggt ttatttgttc cttattttgt gacattaagt gttactaatt
      481 tattacttac tctgtttgtc tttttaattt taattttctt cttggtgttt actgcacaaa
      541 aattagctaa tatcccagga attggagaaa ttgttgagaa atttagtcgt tggattatgg
      601 ctatcattta tatagcttta ggtttattta ttattattga aaatgacact attcaaacaa
      661 ttttaggatt tatattttaa tttagggtgt gattttatat gagttatgaa aatacttgtg
      721 atgtgatctg tgtacatgag gataaagtta acaatgcttt aagtttttta gaagatgata
      781 aatctaagaa attacttaac attttagaaa aaatttgtga tgagaagaaa ttgaaaatca
      841 tattatcttt gattaaagaa gatgagttgt gtgtttgtga tatttctttg atattgaaaa
      901 tgagtgttgc ttcaacttca catcatttaa ggcttttata taaaaatgag gtacttgatt
      961 tttataaaga tggaaagatg gcatattatt ttattaaaga cgatgaaata agagagtttt
     1021 tctctaaaaa tcaggagggt ttttgaaacg agtgaaacga gtttcttctt gtcttgatac
     1081 tataaaaaac aaagttatat aacataagac gacttatcgg aagttatcta tcatcgtata
     1141 ttcggagatg atataatata caggtcgcct atctctcagg cgtcaattca gacgcagaga
     1201 ggaggtgcat tactttgcta atcattttcg ttcacatcat agcaccagtc atcagtggct
     1261 gtgctgttgc gtattatact tattggctta gtaaacgcaa caaataatta ataggcgact
     1321 ttataagcca gcatcgaaaa aatccccaca ctattgcagt agtgtgggga ttggtgcatt
     1381 aattgttaat cattttcgtt atctagatta taacactggt tagtagagga atgcaaaaag
     1441 ataatatttt aagtagtctt tttggatatt atcatatgat atatttatgt aaattgattt
     1501 tttattaaag gagcatatat gagtaatact aaaattaaat atcaaatttt tatttcatca
     1561 acttatttag atttaataga agagcgtcaa gcagtagtag aagcagtttt aaacagtaat
     1621 aatattccag caggtatgga actttttaac tcaagtagta aaagccaatg ggaaataata
     1681 acacaatgga tcaatgaatc agatgcagtg atattattgt taggttctag ttatggaaca
     1741 attgatagtg aagaaaatat tagctataca gaaatggagt acaattttgc taaatcttta
     1801 ggtaaaccta tatatgtttt tgagatgaat aaagcatcta ccgaatccgc ggaaaagtct
     1861 aaaaaattag atgagtttaa gacaaaagtt aaagaaaata gactaattag tccaaatatt
     1921 aatgatattt cggaattaaa aagtaaagta tctcagactt taatacaaag tgtacctagt
     1981 gaaattaaag aaaatagtgg atggataaga gtacaggatt gtggtaaaaa tgtaaaaaaa
     2041 ataaaaaaat tggatgctat acaaagagat ttagatacat atgatttttt gaaagaagaa
     2101 ctttataaaa tattcatgta tgtaaaaact ttttatttcc agcacacacc atttgatgat
     2161 agttatattg aagatataga aaactacctg ctaaattatg aaaataatcc acataaatca
     2221 ttcatgaaaa taaaagttta tgattataaa aactttttaa aacatttaaa aaacatattg
     2281 attttaatta aacaaggaaa aaagccatta gctcaagccg gtcattttaa attaagcttt
     2341 tacgaagagg atttaaaatt gttacaaaaa aattataagg ctacgctagt cgatattaaa
     2401 ggattgaata aacattttta taaggaatgt gataggtgct taaaagcata taatattttt
     2461 attgagggta taaataaaca tttatatgaa tgatataagt acaccctaat catacaaatt
     2521 aagttattga aattacagca attaaaatgt atgattaggg tgtatgttaa ttgtggagtg
     2581 aaaacaaatg attataggct atgctagggt ctcttctatc gatcaaaatt tagaaagaca
     2641 gttagataat ttaaaaacgt ttggcgtgga gaaaatattt acagaaaagc aatcaggcaa
     2701 atcaattaca aatagacctg tatttcaaga ggccctaaat tttgtgagaa tgggagatcg
     2761 attcgttgtg gagtctattg accgtttagg tcgtaattat gatgaagtca ttaataccgt
     2821 taattatcta aaggataaag aagtacaatt gatgattacc agcctcccaa tgatgaatga
     2881 agtgattggc aatcctttat tagataaatt tatgaaagat ttaattatac agatattagc
     2941 aatggtttct gagcaagaaa gaaacgaaaa taaacgtcga caagctcaag ggattcaagt
     3001 tgcgaaagaa aaaggagttt ataaagggcg gccacaatta tattcaccta atgctaaaga
     3061 cccacaaaaa cgtgtcatct atcatcgtgt tgtggaaatg ttagaggaag gcaaaacgat
     3121 tagtaaaatt gccaaagaag tgaatataac aagacaaacg atttatagaa ttaaacatga
     3181 cagtggtttg agttaataaa ctattatcaa gttctaatca taaaaaaaga gagagcttat
     3241 tcgctatctc ttttcctata atttttaata aagaattatg taaatatata taaataaatt
     3301 atattcaacg ctttcttgtc atcatataat atattatagc attaattact agaaaaacga
     3361 aaatcacgct aaacacaata aatttagttg caagtgcaat atttgaaact aatgtaaaga
     3421 taaacaatat aatacctata attgctatta tattgaatgt ttctaatttt tctctcatta
     3481 taatcctccg tttcgactga gagctctata agtatttaaa aaatgtagtt attataacca
     3541 tattttttaa atgttcaatt gattttgtag gtgaatatat agttgtattt acccatataa
     3601 gaggattttc ctattatcta ttcttcttta cattcctata ctaaccagta ttataatcta
     3661 gttaacgaaa atgattaaca aaataatgca tctcctctct gcgcctaaat ttgacgtctg
     3721 agagatacgt gccttattat atattatgcc attttcgaat acacgataat agctaattcc
     3781 tataaatctt cttggacaat aagaaggaca ttctgccaaa aaaccgctag tctttactaa
     3841 cggtttcatt tacattcttt tcttttttat ggttgaagct taattacata tctataatat
     3901 atctttaatg taattgtttt accatacttt tagagatatt tactgtttag tcagagctaa
     3961 atctcttcca tcttcactct tatcaggatg tttaaataat tttttatcta tttccctatc
     4021 attaattgat tgctgcaaat ctttttcagt taaagtgatg tcgtctttat tcttctcttt
     4081 tatttcataa acttcatagt atttgctatg atcatagtct atactacggt catcataaaa
     4141 atgaagttca tctttatatc catctggaaa ttctgaggag tctcgttcca gcactaaaaa
     4201 tttcttgcct gcatccttct taatttcgta attaaacggt gttgagtctt taatcatgtt
     4261 gtctggagca ccttccgtcc atgacatcat tgcacgatct ttgccgttaa acgatatgct
     4321 ataatccgta tcttcaactt tatatttctt gttcgctaac ggatcatcat gaacaccttg
     4381 acaagcgcct aatatcacac tacaaccaac aacgattgct attaaacctt gttttttgtt
     4441 caactagcac acacaccttt tacttcaatt tttttaacca ttcatctttt tctttctcac
     4501 taacgctacc taatgttttt aagaaataac ctttattcca aaacggttta ctttctatac
     4561 gttcatctaa aatttcggga aaatattttt taatattaaa cgatgaagta gacttaaaaa
     4621 ctgcaacaaa gtcagaaaca gaagtttgtc ctttgtctac agaaaaggat atttctatat
     4681 aaaaatcatg atattcccaa taaagcaaat gaacactata tgtttttgcc acttttaaaa
     4741 agtgtcgttt caaaagtgtt gattgttttt cagaaatata agatctcttt tgattagtag
     4801 ctataactaa ataatagttt aaatcatata acgattgcgt tcgcttattg taaaacatct
     4861 gaccaccttt tcaatcttac atattaattt taacaaacca ttataaccac tgcaatatca
     4921 ggttattccg ttttacaaaa acttaacata gtgatttatt ttaaatcctc aaaaattatt
     4981 gcgcttttcg gtaaaatggt cttcttttgc tcgattttac cagaattttt atatttaact
     5041 attctttcaa taagtaattc aaagcgtctt tcttccttga tggtagctat tatacgtttt
     5101 tcagtaaata gataaaaaat tacttgttgt ccgatataat cttgatacat tgattcgtta
     5161 tatgtatttt tagtcatttt gacatctcct tgttatagtc caaacatatt actttgagac
     5221 atattttgtt gacgtttagt aagaacatgt atttcttaaa attcagattt aatacagcac
     5281 attaaatagc tttgcatata ttgaacagat tcattctttt tctttaagat aattctaaaa
     5341 cgttttagta catcaataag ttcatcttca atattttcaa gtgtataagg cataacaatt
     5401 tcatcagtac gttcatcatg aaaagaacgc tttgctttta agataatacc ttttataatt
     5461 tgaatttctg ataaagaata attggttctg ttgcaaagtt aaaaatcatg taatatttat
     5521 actgaaaaac gaaaaataat taatactaac aaaactcttt actgttaaaa caatatttgc
     5581 ttttatagtt ttgtatagat gtgcatataa ttttaacttt gcaacagaac ccttttttta
     5641 attacagaat taatcaggtt ttaagtcttt cttttttgtt tttgatcttt tgcattccta
     5701 tccactatgg tgttataata tatttaacaa gagcttcgtg tattatatgc accaatcccc
     5761 tcactactcg caatagtgag gggattttta ggtgttggct aatatcgcct attttttctt
     5821 attgttacgt gtacgtagcc aatgcgtaaa taacgcaaca atacaaccac tgatgactgt
     5881 ggtcgtgatg tgaacaagag cttcgaccat ctgatatgca cctcctctct gcgtctgaat
     5941 tgacgcctga gagataggcg acttatatat tatatcatct ctgaatatac gatgatataa
     6001 gacttctgat aagtcatttt atg
//
LOCUS       DAMZLE010000132         5997 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMZLE010000132 DAMZLE010000000
VERSION     DAMZLE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.133"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..1195)
                     /locus_tag="POB92_002236"
     CDS             complement(<1..1195)
                     /locus_tag="POB92_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579703.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSF"
     gene            complement(1210..1524)
                     /locus_tag="POB92_002237"
     CDS             complement(1210..1524)
                     /locus_tag="POB92_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDI6579704.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(1502..2320)
                     /gene="recX"
                     /locus_tag="POB92_002238"
     CDS             complement(1502..2320)
                     /gene="recX"
                     /locus_tag="POB92_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI6579705.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(2581..3390)
                     /gene="sgtB"
                     /locus_tag="POB92_002239"
     CDS             complement(2581..3390)
                     /gene="sgtB"
                     /locus_tag="POB92_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDI6579706.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(3729..4244)
                     /locus_tag="POB92_002240"
     CDS             complement(3729..4244)
                     /locus_tag="POB92_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6579707.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(4375..4536)
                     /locus_tag="POB92_002241"
     CDS             complement(4375..4536)
                     /locus_tag="POB92_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579708.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            4760..5911
                     /gene="yfkAB"
                     /locus_tag="POB92_002242"
     CDS             4760..5911
                     /gene="yfkAB"
                     /locus_tag="POB92_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDI6579709.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
BASE COUNT         1911 a         1038 c          808 g         2240 t
ORIGIN      
        1 taaaactatg cgtttgatat aaacctgccg ggttaaaata ttctaattta tctttactaa
       61 catagtaagt cttattatcg gcggtaacac gataattttt actattttcg ccagtaattg
      121 taactacact atattttgga attgtgactt gtttatcccc ttgcatatcc acactgccat
      181 taaaagcaat tccataagct aacttttcgc catatggatc tttattttca ttttgcatcg
      241 tagcacgatt cgtaaaatct gttatcgaaa taattcctat attattgaaa atgagcaaca
      301 tcatcaatgc taagcctaaa atagtacctg aggctaaagt ccaaaaacgc actaaaaact
      361 taggcggttt tgcatgatta tagcgctcaa cacgtttgaa attataggtc atctctggta
      421 ttcttgtacg attctttttc caatctaaat caaagttttc aatttcttct tttgagttta
      481 gacttaatcg atcacgttca tgttctttaa aagatggaat aacttgttta agtgaccctt
      541 ccattcttaa ttgaccatgt gaaaaccaag ctatgtagtt actcacttgt gaaattttat
      601 caatatcatc accaattgac acaatcgtta aattattttc aatataatca tttgttaatt
      661 caatcgcacg ttccataaat tgtggtgtta aatagtcaat aacatgattt aaaataataa
      721 tatttgattt tgatgagcgt gcaatactta atagtaattg agcgtatgcc gctttcgaaa
      781 tatggttaac cggcttcgtt ttataatcac ctaaatgtgc atattgaata atctgttcag
      841 ctttatgatc attaatttca tatggaaata gttgaactaa ctgcgctgta taagcttcaa
      901 cagtttgatg aattaacgat tgatcttcaa tatatccgta gaacaaatct tcagtacaaa
      961 ctactttacc tttatcaggt ttaattgcac ctgccaacaa ttgacctacc aacgctttgg
     1021 aagattcagg ttcaccaatt atacctaatg cttctccttg ataaatatgt aaactaatat
     1081 tgtttaaatc gatatcttca gcatcatatc caaaaggtaa ataccatttc ttattctgtt
     1141 tattcctata gtagtgtgtt acttttagta actttaaaac aattgaactt cccatctatt
     1201 ttcatccttc tataattgta ataacgctat aggtaatcct tcttcgggtt catcgctatt
     1261 aaatctaaac ccccacgcaa aaataccttt taatctttct actttaaaat attgattact
     1321 gccatcagtc agtttataaa ttttaccaat ttctatcttt tttctgtcta ctaagtaact
     1381 ttcggcaaca attactttac tttgataaac gtcatattca tttaaaatac cattcatctc
     1441 agcttttctc attttctctt tataaagttg tatttcgtgt cttaattctg gctcagacat
     1501 ctcactcaat ttcttctgtt ccatcggcaa taccactttc ttctaattta gctttaattt
     1561 tatcatattt atatcctttt ctcataaggc cttcgatagt ttttgaaatt aatttctgtt
     1621 gcgtgtactt ctttcgattt ttattataaa ttttttctaa atctcgttgt aataaatcgt
     1681 ctaaaaccgc ttcatcttgt gtaaaatcca tttcattcaa tacagcatga atggtttcca
     1741 tttcaaaccc tttttgaatt aaagattgca ttactttttc tttaacttta ttttgtggcc
     1801 ctttttttgt tttagatatt ttttcagcaa tttggataat atcatcaagt tcctgttgtt
     1861 ctctataaag ttctgtaaat atttcaatga tatttggttc tataccaagt tgatatagtt
     1921 tttgttgata aattttaggt cctttatctg tcgtgcgaat cattgtattt tttaaacttt
     1981 ccgcataatc ttgatggtcg attaactttt cgcgataaca atattcaatc acttcagaaa
     2041 tcgcttgctc tgatatctct tctttttgta aatattgtat aacttctttt tcagttctct
     2101 ttttatatga taaatattgg attgctttgt ttaaacctat gcgataatga tcatactttt
     2161 gaatctctgc catgtcagca gcttcaagtt gttgcccttt ttttaaatta aatttgacta
     2221 atgtatcgat atctataccc atttcaaatt gttcgtctaa aaaaagatta aaacgttctt
     2281 tgtttttctt ttgaacttct attttagtaa tcttcggcac tggatcaccc cgttactttc
     2341 tcaagtttta atacaatact cttttattat tcaataagcc acttcctata gcaaatgttt
     2401 aactttaaat atttttcgat gctaacaaaa aatcacacta tcatctttta aaatgaaagt
     2461 gtgattacaa gcaaatctgt aaaatttata aagcagaaac aattcaactt tatcattatg
     2521 acatttcaat taaaccttct acattatagt tccaagcatc ttacacatga atgcaagtat
     2581 ttaacgattt aattgtgaca tagcctgttg atattgtgtt tcattgatat aattttgttg
     2641 cttcattttt tctaagttcg tgcttacacg ttgcgtaaaa ttctctgaca tattattgat
     2701 attatatacg ctaggtgcat tgactttact agctaaaata gcgctttgta aaactgttat
     2761 gtgagacatt gttgtactat ttttattcac ggttgttcca aagtaatggt ttgctgcgcc
     2821 ctcaagcgta tattgattat ccccaaagta aatattattt aaataaaagc ttaaaatttc
     2881 gttcttatta tattgttttt caactcgatg agctacaaat aattctttta cttttctagt
     2941 aaatgaacga tcattatcat aaaaataatt tttgacaact tgttgtgtaa tggtactacc
     3001 accttgcaca tctctgtcgc taatcgttga aaataaagct ctagttgtac ctttcaaatc
     3061 gaatccatga tgattgtaga atcgttcatc ttccattgaa ataaaggcac ctttaacata
     3121 ctctggcatg ttatcagctg acacaaaact acttttattt tcaatttttc ttagttcatc
     3181 cacattatcg cgtgtagata aaaaatacat gataccaata aacaatgcga taatgattag
     3241 aatggttaat aatattttta atagtattcg tttacttttt ttctttttcg gcggtttgcc
     3301 aactggttga taatacgtat tatagtgagg ttcgtgtttc atatgctcaa aatgttcatt
     3361 tgagtttgag tacctatcgc ttcttttcat gcgtttgctc cttcttttaa aactcactta
     3421 gtatatacct tgagtttacc agtactatca caaataggct acactttttg ggaaaatcag
     3481 tccaagggct tacaatcgta tacgccatca tacttacttt ttgttttttg aaaagattat
     3541 agataaatca ttgcgatttt aaatattaat catgtcaaat attgttatat tttataaaaa
     3601 taaaagacca tccctattaa atgccaatag agacgacctt ttatttgtta ttcatttttt
     3661 aaaactaaaa tccatatttc atttcaaacg aaaatatata aattttaaca attgataacc
     3721 gcaatactct attgtaattg cttaacgatt tctcgattaa aatcatctaa atcgtctggt
     3781 actcgacttg ttacaatatt gttgtctaca actactgact catcaactac atgtgcgcct
     3841 gcatttgata aatctttgcg tacatttaat actgctgtta acgtacgacc ttttaaatcg
     3901 tctgtatcta ttagtatttg tggcccatga caaatggcaa atgttggtac atcattttta
     3961 gtaaagtatt tagcaaatgt gccatatcga ccttctgtat ctccacgtaa atgatctggt
     4021 gaaaatcctc caggaattaa taatgcatca taatcttctg gtttagcttc tgcaatgcct
     4081 acatcgacag taactttttc accgtgttta ccaacaactt cactatttgc agtatctcca
     4141 atcactacag tattaaagcc tgcattctct aatgcctctt tagggcttga atattctata
     4201 tcttcaaatt cgtttgctag aataattgct acttttttag tcattgaaaa tcacctttct
     4261 atatatcatt gatataatta ctatagacaa gtaaatcagt gattaaacat acaagatata
     4321 aaaattatta agcgactgtc gcgatatcta accctaacac atcttatgtg gcatttactt
     4381 agatactaat ttaacctttt cttcaagctg atctaacaat ccaatccatt catctatatc
     4441 ttcaacacgt acttcatcag gatttacatg atcgatatcc tcaataaact tatttaaacg
     4501 cgcttttatc tgttcgattg tttgctgttc attcataaaa agttaactcc ttttattttg
     4561 ttttcttttt cattattatc ctaacagaaa ttgcgttaaa gcgatataat cttagctata
     4621 tttatgacat tcaaattatt ttgactttta aaaatcccct tttcaattaa ctaaaattaa
     4681 gagataattt gttacgagtg ataatacgaa gtggtatcat accgatatga accaaataga
     4741 aagaaggaag tttaagacga tgaatagcgt caaattgaag caacctgtta gcatttacaa
     4801 tgatccatgg gaatcatata acgatgttaa agaacatggc caattaactt taagtaacat
     4861 cgaatttaca actacaaatc tttgtaatat gcgttgtagc cactgtgcag ttggttatac
     4921 tttacaaact gtcgaccccg agcctttaga tatggactta atttatcgta gacttgatga
     4981 aattccaaat ctgcgaacga tgtcaattac aggtggcgaa ccaatgtttt ctaaaaagtc
     5041 tattagaaat gttgttaaac ctctattaaa gtatgcacat catcgaggta tatatacaca
     5101 aatgaattca aacctaacat tgcctcaaga tcgttattta gatattgctg aatatatcga
     5161 tgttatgcat atctcacata actggggaac aactgatgaa ttcgcaaatg ttggctttgg
     5221 cgcaatgaag aagcaaccac cgttaaaagc taagttaaaa ttatatgaac aaatgatttc
     5281 gaatgcacgt acattatcag aacaaggaat gtttgtatct gcggaaacaa tgctcaatca
     5341 aagtacgcta ccacatttac gaaaaataca tcaagaagtc gttcatgata tgaaatgtag
     5401 cagacacgag attcacccta tgtatccagc tgactttgca agtcaattaa atgtgttaac
     5461 tctagcggaa atgaaaaaga caattcatga tatattggat ttcagagatg aagatatttg
     5521 gatgttattt ggtactttgc ctgtgtttcc atgcttaaaa gatgatgaag atcaaaagtt
     5581 actatcacgt ttaagaactg ctaaaaatgt aacgactaga aatgacccgg atggccgtag
     5641 tcgtttaaat gtcaatgtat ttacaggtaa tgtaatcgta actgatttcg gagatgaaac
     5701 aggtacaatt tcgaatatac aaaaagataa attaacagat gtatttgata aatggttatc
     5761 ctctgatctt gctaaatcat taaattgtca ttgttccgag tttagttgtt taggaccaaa
     5821 tgttcttgtt aaaaatatgt actatccgaa tatggatttt aaagataatg agcgtcatat
     5881 gcacaaacaa ccacaaatta tacaatttta aaaactctta attatgcgga gaagcacttt
     5941 atcgataagt agtctccgca tattttaatg ctattataaa ataaaaaaca attaatt
//
LOCUS       DAMZLE010000133         5979 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMZLE010000133 DAMZLE010000000
VERSION     DAMZLE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.134"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            397..1308
                     /gene="rapZ"
                     /locus_tag="POB92_002243"
     CDS             397..1308
                     /gene="rapZ"
                     /locus_tag="POB92_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI6579710.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1305..2300
                     /locus_tag="POB92_002244"
     CDS             1305..2300
                     /locus_tag="POB92_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDI6579711.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2411..3355
                     /gene="whiA"
                     /locus_tag="POB92_002245"
     CDS             2411..3355
                     /gene="whiA"
                     /locus_tag="POB92_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDI6579712.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3928..4515
                     /gene="clpP"
                     /locus_tag="POB92_002246"
     CDS             3928..4515
                     /gene="clpP"
                     /locus_tag="POB92_002246"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDI6579713.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4758..5660)
                     /locus_tag="POB92_002247"
     CDS             complement(4758..5660)
                     /locus_tag="POB92_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDI6579714.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGRLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT         2135 a          841 c         1104 g         1899 t
ORIGIN      
        1 gcacattatc gtaagctgac ttttcgtaag cttctgtgtt ggggccccgc caacttgcat
       61 tgtctgtaga aattcttttc gaaattctct gtgttggggc ccacacccca actcgcattg
      121 cctgtagaat ttcttttcga aattctctgt gttggggccc ctgactagag ttgaaaaaag
      181 cttgttgcaa gcgcgttttc attcagtcaa ctactagcaa tataatatta tagaccctag
      241 gacattgatt tatgtcccaa gctcctttta aatgatgtat atttttagaa atttaatcta
      301 gacatagttg gaaataaaaa taaaacatcg ttgcttaatt ttgtcataga acatttaaat
      361 taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga aaaagaaaaa
      421 agtaaaagtg aactattagt tgtaacaggt ttatctggcg caggtaaatc tttggttatt
      481 caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt gttattacct
      541 aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc aattgcaatt
      601 gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa agttaaaagt
      661 gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa attaatttca
      721 agatataagg aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag atccttaatt
      781 aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc taattttgtt
      841 atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg atactatgaa
      901 gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa acatgggatt
      961 cagatggatg cagatttagt atttgatgta cgatttttac caaatccata ttatgtagta
     1021 gatttaagac ctttaacagg attagataaa gacgtttata attatgttat gaaatggaaa
     1081 gagacggaga ttttctttga aaaattaact gatttgttag attttatgat acccgggtat
     1141 aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg acaacatcga
     1201 tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga atataatgtt
     1261 tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga caaataaaag
     1321 ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta agagaattcc
     1381 caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca gggaaaatca
     1441 gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca gctttaagtg
     1501 attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat caaattagcg
     1561 gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat gatttcggac
     1621 atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt ccatctacaa
     1681 atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt ggagaaacaa
     1741 atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac gatgtgcaac
     1801 caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt ggaccagggt
     1861 cattatatac gagcgttatt tctaacttat gtgtcaatgg tatttcagat gcgttaattc
     1921 attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg gaaacagatg
     1981 gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa ccgtttattg
     2041 attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa tatgaagaaa
     2101 aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc ataaatgtaa
     2161 aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat aatactaaag
     2221 tgttatcgac aatgatttat gacatagctt tagaattaat tagtactatt cctttcgtac
     2281 caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata atagagctgt
     2341 gtaaaaaaat gaaaatagac agtggttcta aggtgaatca tgttttaaat aagaaaggaa
     2401 tgactgtacg atgagctttg catcagaaat gaaaaatgaa ttaactagaa tagacgtcga
     2461 tgaaatgaat gcaaaagcag agctcagtgc actgattcga atgaatggtg cacttagtct
     2521 ttcaaatcaa caatttgtta taaatgttca aacggaaaat gcaacaacgg caagacgtat
     2581 ttattcgttg attaaacgtg tctttaatgt ggaagttgaa atattagtcc gtaaaaaaat
     2641 gaagcttaaa aaaaataata tttatatttg tcgtacaaag atgaaagcga aagaaattct
     2701 tgatgaatta ggaattttaa aagacggcat ttttacgcat gaaattgatc attcaatgat
     2761 tcaagatgat gaaatgagac gcagttactt gagaggagct tttctggcag gtggctcagt
     2821 gaataaccct gaaacatctt cgtaccattt ggaaattttt tctcaaaatg agagtcatgc
     2881 agaaggctta acgaaactaa tgaatagtta tgaattgaat gccaaacatt tagagcgaaa
     2941 aaaaggaagt attacgtatt taaaagaagc ggaaaagatt tcggattttc ttagtttgat
     3001 aggtggctat caagcgttat taaaatttga agacgtacgt attgtaagag atatgcgtaa
     3061 ttctgttaac cgactcgtta attgtgaaac ggcaaatcta aataaaacag ttagtgctgc
     3121 gatgaaacaa gttgagagca ttaaattgat tgataaagaa attggtattg aaaatttacc
     3181 agacaggttg agagagattg ctagaattcg agtagaacat caagaaattt cgttgaaaga
     3241 gcttggagaa atggtatcaa ctggtccaat ttcaaaatca ggtgtaaatc accgattaag
     3301 aaaacttaat gatttagccg ataagattag aaatggtgaa caaatagaat tataagtaag
     3361 aaggtgtttt tggtagtgaa ttatcaaaaa cactttttta tgtttaaaaa gtgtttaatg
     3421 aataatagtc gactaactat taaattgagc tggaaagttt aatgaaggaa attcaataac
     3481 caagtgatgc ctaattttct cattaaatag catcatatgt atcaatttgg tcataagaaa
     3541 atatgaatgt gataattaat tgagtccctg aaagtccctg atcatagaca aaaacaaaat
     3601 cagcataaat aagaatcccg acattgcggg attctctgta ttgaaaagta ttgtatttta
     3661 ttaaaacagc ctccttgaag ggaattgaac ccctatctta agaaccggaa tcttacgtgt
     3721 tatccattac accacaagga gcaaatatgt aatcatatct ctgtactatg agaagtaaaa
     3781 taagtacaat ctaaaataga tattatcagt ttacaaagga aagagaaaag cgtcaaacaa
     3841 tgtaactatt taaagtcaaa gtgtttgacc aagtttgact taatatgtaa aataatgagt
     3901 aacagttatt acaaggagga aatatagatg aatttaattc ctacagttat tgaaacaaca
     3961 aaccgcggtg aacgtgcata tgatatatac tcacgtttat taaaagaccg tattattatg
     4021 ttaggttcac aaattgatga caacgtagca aattcaatcg tatcacagtt attattctta
     4081 caagcgcaag actcagagaa agatatttat ttatacatta attcaccagg tggaagtgta
     4141 acagctggtt ttgcgattta tgatacaatt caacacatta aacctgatgt tcaaacaatt
     4201 tgtatcggta tggctgcatc aatgggatca ttcttattag cagctggtgc aaaaggtaaa
     4261 cgtttcgcgt taccaaatgc agaagtaatg attcaccaac cattaggtgg tgctcaagga
     4321 caagcaactg aaatcgaaat tgctgcaaat cacattttaa aaacacgtga aaaattaaac
     4381 cgcattttat cagagcgtac tggtcaaagt attgaaaaaa tacaaaaaga cacagatcgt
     4441 gataacttct taactgcaga agaagctaaa gaatatggct taattgatga agtgatggta
     4501 cctgaaacaa aataattcaa agtaaagagt agactaagca gtctgctctt tttgtatgag
     4561 taaaccgagg tgtcaataaa ttgtttacta tactttgagc ggaaatatga ttgaatgaag
     4621 ctagttgaac cgtaactata tgaaatgttc ccttcaaagt agacattgaa aggaacattt
     4681 caatcctttg tttgtaagtc gctctagaca ttacatttag tacatatgtt gtttctaatg
     4741 ctcattaatg gtattgatta ttctttaatt aaatcttcaa gtgccatttt taaattacta
     4801 tatttaaatt ggaatcccaa tgcttgaatt ttattaggta atactttttg agtatccaat
     4861 actactgttg acatttgacc aagtatgaga cgcattgcaa gacttggtgc ccaagtttca
     4921 tggggcttat gcatagctct tgctaaagtg tagccaaata aattttgacg ctcaggtatt
     4981 ggtgcagtta aattaaacgg accactagct gactcgttat ttattaaaaa taaaatagct
     5041 tgaattaaat cattgatatg aatccatgaa taccattgtt ggccagaacc taatctacca
     5101 ccaatgtaat atttgtatgg tagtttcatt gtttgtaacg caccgccttc attcgataaa
     5161 atcataccga aacgaccgat gacaactcgc gtacctaatt gttcaaattg ttgtgcgaaa
     5221 cgttcccatt gatacacaat atctgataag aaatcaaatg gtaaagtttt ataaacttct
     5281 gtgtaactca taaataaatc aggaggatag taaccagtgg cgctagcatt aaataaaact
     5341 ttaggtgctt tattacgtga tttaaacaat tcatataaag cttgcgtaga ttgaattcta
     5401 cttagcatta gcgtttgttt atattccggt gtccatcgtt tattcagtgt agcacctgct
     5461 aagttgatga ccacatcgat attttgagga actttgtgtt cccacccaga tttagcccag
     5521 ttgacatatg aaattttctt atcatttgaa atttggtcgt gtcgcgttaa tatcgtgata
     5581 tgtgaatctg attttttaat ttcattaact aattgagatc caaccatacc agtcccacca
     5641 gtaattaagt attgtttcat tatcattcac cccatgtaat tttgtattta gttactattt
     5701 aataatcatt attgttgttc aaaggttata cattatttag acaataatat gtcaataact
     5761 ttttttgaat tgtattttaa taaaaatgat ataagttata ataaaggtgt accttcgata
     5821 acgaataaac atctcttaaa agtatgtgta aaacgctgca tgatacaaac gaaggtaaaa
     5881 atttgactcc ctttagtagt ggacccgtac gttaatcgtg cgggtcgttt ttatttattt
     5941 cttattccca ttatacatca atttaaagca ttaattttt
//
LOCUS       DAMZLE010000134         5943 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMZLE010000134 DAMZLE010000000
VERSION     DAMZLE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.135"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(284..856)
                     /gene="raiA"
                     /locus_tag="POB92_002248"
     CDS             complement(284..856)
                     /gene="raiA"
                     /locus_tag="POB92_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI6579715.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(917..1591)
                     /locus_tag="POB92_002249"
     CDS             complement(917..1591)
                     /locus_tag="POB92_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDI6579716.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1584..2666)
                     /locus_tag="POB92_002250"
     CDS             complement(1584..2666)
                     /locus_tag="POB92_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6579717.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(3030..3896)
                     /gene="fakB1"
                     /locus_tag="POB92_002251"
     CDS             complement(3030..3896)
                     /gene="fakB1"
                     /locus_tag="POB92_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDI6579718.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            4040..4681
                     /locus_tag="POB92_002252"
     CDS             4040..4681
                     /locus_tag="POB92_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDI6579719.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(4853..5908)
                     /locus_tag="POB92_002253"
     CDS             complement(4853..5908)
                     /locus_tag="POB92_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDI6579720.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
BASE COUNT         1988 a         1035 c          840 g         2080 t
ORIGIN      
        1 cattctgttg tcaacaagtt tatcactaat aaatatattc tcaatacgca attatacttc
       61 ctaataaatt atattataaa tattttacga ttttcgactc ggactataca aaagactgac
      121 atactattat taacttaaca ttcaaatata tacatccatt aacattagca tagtcactat
      181 gtttcattca acaaattaca ttatcgaact atgaaatagt cataatttgc ttttggagta
      241 taaaaaagca cttgtgcaaa aacacaagtg ctttaaactt aatttattgt tcactagttt
      301 gaatcaagcc atatttaccg tctttacggc ggtaaacgat acttgttcca tcagtttctc
      361 tgtctgtgaa tacaaagaag tcatgaccta atagattcat ttgtaatacc gcttcttctg
      421 aatccattgg ttttaagctg aattcttttg aacgaataat ttctatctcg ttatcatcgt
      481 aagcgtcatt atcaacttgt gtttcttgca tttcttgtaa ttcggcaaca aacacttctt
      541 gatctcctcg atcacggctc ttacgattaa tacgtgtttt atattttcga acttgtcttt
      601 caagtttatt attaattaaa tcaatacctg cgtataaatc atcgtttcgc tcttcagctc
      661 ttaacgtaac atttttcaat ggaattgtta cttcaatttt agtagctgaa tttgaataag
      721 ttttaacttt aacatgcgcc actgcatttg gtacgtcatt aaaataacgt tccaacttac
      781 caattttttc ctcaatatag ttgcgaatag catctgtgat agtgaggtta tctccatgaa
      841 tttcaaatct aatcatagta atctctcctt aaacctcttt attggtaact ctttattata
      901 ttttaacatt tttacgctat cgtgcaaacg caaacacttt gaattttctg atatttttag
      961 catataattt acaccctgca cgatgaattg ttaatccagt tgtataaata tcgtcaacga
     1021 gtaatatttc cttgccattt aaatctaatt ccgtatcaat aataaatgga ttttcatctg
     1081 ccagacgctc tttcttagtt aaatgagact gttttggtct atttgacatc tttaaaatct
     1141 tatcaaagcg aatcccttta gcttttagta ctgcttctac cgggttaaat gttctagata
     1201 aatcatgtgc cggcgaagaa ggaattggca caatatagtc ataagatgtt tgtggtattt
     1261 caatcaaatg tgccaataat tcacataaat aatagtcttt caaaaattta tactgatgta
     1321 tcatctcttt cattaaaccg tcatattgaa attgacaata taattgttcc attaaattaa
     1381 agtgtgccga tagaaacttg cagtctaaac aatacgcttc atcttgattt aagtgtttta
     1441 agcaccttga acatcgcctt gctttaatat caagtttaat attgtcccaa ttctctttgc
     1501 atctgtcaca taatctatta ggtttcttga acaaattata aatggttata ttttcatata
     1561 acttagcacc acaactcaaa caattattca tcaatccaac ctctttttaa tgctaatttg
     1621 ttcatccttt gaatctcttt tttagcttga atcatattca tacttactcc ttcatgaaaa
     1681 aacaatactt ttccagtagg acattctaat tttcgtccaa cacgtccagc aatttgtatt
     1741 aaagcctctt gagtatattg atgtgcatcg ataacaacaa catccaaatt tgccattgta
     1801 aatccacgtt ctaatattgt cgtagtaaaa atgacatcga aatgtccatt ccttaattgt
     1861 tcaacttttt caaagcgaaa aacatcctcg ctatggacgt atgttaattt agtaattttc
     1921 tgcttataaa ccgaaaatgt tttaatcatt gtttctatat tgttaaaaaa caccagtgta
     1981 taacgttgat tattaatttg atcttgtaaa attcggtata acattttctg aatcttatta
     2041 ttattaagtt tgaaataacg atatttagga actggaagtg attttttatg aaagcgagct
     2101 ggcaatttaa ttatattttc gtggggaatc tctgacagaa gttgtttcgg tggtgttgct
     2161 gtcatataaa ttgttgcatg ttcaacttta gaagatgact tcaatgcttg ttgtagattt
     2221 ttatccattg ataaaggaaa ggcatcgact tcatcaataa aaatagtatc aaagtgctgt
     2281 ttgaatcggt aaagttgatg cactgtgcat acaacaaaat gcccttcaaa ttgttgtctt
     2341 gattgctggt gtagtatgtc tatatcttca ttaagaaatg cgtctttaat acgtttacta
     2401 atttctacaa caacatctac acgtggtgac acaatagcta tattatctcc ctgtattctt
     2461 gcatattgaa tgccttgaaa catcatttct gtcttacctg cacctgttac ggcatataac
     2521 aaaatcgttt gtctctttct aatggctcga acaatatgtt ctgaggcata agactgctgt
     2581 tccgacaatt caaatgagag atgataatat gcttgtgatg aactttgcga actctctgtt
     2641 attttatatc ttgttacatt atccattcga cccagtgata tacaatttct gcaatataca
     2701 attttcttgg aatgtactga ggaatcatac tgataaaaat gctttggatt tgtactttca
     2761 cattgaacac aataatagtt tgaattttgt ttgatgacac ctgttacttc ttttttaatt
     2821 ttttcattag taagtgaagt atgaggatga attaatttcc cgtaatgttt aatgcttaaa
     2881 acctcatttc aacgtaaaaa agagcctaat attataatga ttttcttata tctttagcat
     2941 tttggctttc aaatgatgaa catttaaagc catgtatacg agagataatt tccaattaca
     3001 atatagactc ttttattaaa attttataat tatgttaatc ttatttttct gccaacatag
     3061 cctaaaccta atccaccaga acctaaatgt gctgcaacaa ctggaccaaa ctcagagtat
     3121 gctacttgat aagctgaagg acaatcatct tgtaactttt tgtataacgc ttgaccatct
     3181 tcgaaatgat ctccatttat gacaaataaa gttacttctt caaagtcttt tacaatatct
     3241 aatactttct tttctaatgt ttgaatggca cgctttttag tacgaacttt ttcttctggt
     3301 ataatcttgc catcttcaaa cttaagaact ggcttcattt tcaataatgt tccaacccat
     3361 gcttgagcac cagtaattcg accacttttt tgtaaattct ttaagtcatc aacaattaaa
     3421 tatgcacctg tgtgttcacg catattagtt aaatcatcaa taatttgctg tggttcgtat
     3481 ccttcttcaa ccatttcaat agcacgtaat acataacatc cttcaatcat tgctgcaagc
     3541 ttactatcaa atgcatgtac attaacacct tcaaccattt cccctgcttg ataactagat
     3601 tgataacttc cactaatccc actggataag caaatgacaa tgatatctgt gtaaccctga
     3661 tctcttagca tttcatattt agtaatccat tcgccaattg ctggttggct tgttgttgga
     3721 atcgtttgag acgatgccat tttattataa aattcttcaa ttgctatttc attactttct
     3781 gtaaagttct tcccatcatc aaaagtcaca cttaatggcg ctatttgaat attatattta
     3841 tcgattaagt cctgcgacag ataacttgta gaatcggtca tcacagcaat tttcatagtc
     3901 gtcctcctaa aatatactca tagcaatgat acaatatttc agttaaaaag aaaatggcaa
     3961 tcaaacgatt tatagtcatg tcattttcag tgtataatga agattaaata atcttaagct
     4021 tatgaattaa ggagttatta tgacacaaaa tatcattact gttaaaaaag aacacattat
     4081 tgaaaatgtg attagtaaat caagattcat tgctcatatc aaacctgttc aaaatgaaga
     4141 tgaagctaaa gcatttattg ctgcaataaa aaaagaacat aaagatgcca ctcataattg
     4201 ttctgcatat actattggac ctgaaatgaa tattcaaaaa gcgaatgatg acggtgaacc
     4261 gacaggaact gccggtgtcc caatgttaga catattaaaa aagctcgatg ttcataatgc
     4321 gtgtgtcgta gtaacaagat attttggtgg tattaaatta ggtggtggcg gtttaattcg
     4381 cgcctatagc ggtgctgtta gagacgtcat ttatgatgtc ggacgtgtcg aactacgaga
     4441 agcagtacca tgtatagtaa cactgaatta tgatcaaact ggtaaatttg aatatgaatt
     4501 agcttcaact cattttatgt taagaaacca aacttacact gataaggtaa gctactatat
     4561 cgatgtcgtt aaaagtgact atgaagcgtt tattaacttt ttaaatcaaa tgactgcagg
     4621 aaatttcgat ttgactgaag aaactccaaa gcagttacct ttcgatatac caactgaata
     4681 aaaataaaaa gcactatcat aagacggtaa atatcaattg cgtattatta aacgacaatt
     4741 gaagtacacg tttatggcag tgctttttta tatttgaaat ttaatacata aaaaattgca
     4801 aagagtaaag ccatataata caaattatcc catacagcta tgctttcatt ccctattcct
     4861 ctttgtgtga cgatttccga ctaattaaat ttaatattgg tcggtagttg ttatctatta
     4921 atcctgtaaa ttcaacaatt aattcaatac taaatatgat taatacaaac attaatacaa
     4981 cacctaatgg tggcgataca tacaaaataa tgctcgaaag actaaataaa atagagattg
     5041 aatagattaa taatactgtt tgtctatgtg tgtagcctaa agctaatagt ttatgatgca
     5101 aatgcgattt atcagcttgc attatatgct gccctttttt cacacgtcga atcattgcga
     5161 acaaagtatc aatgaatgga actgctaaga taacaattgg gaagaacaat gcaataattg
     5221 taatattttt gaatccgagt aaagaaagga atccgatgat aaatccaatc attaaagccc
     5281 cactatcacc taaaaatatt ttggcaggat ggaaattgta aaataaaaac ccaattaaag
     5341 agcctaataa aacacaacaa atcatcgtta tgaaaatatt agcttgtaaa attgcaatga
     5401 atcctattgt aatgagtccg attgcagaaa cacccgacgc caaaccatcg agtccatcaa
     5461 ttaagttaat tgcatttgta atagcaacaa tccaaatcac agtaattgga atactaagaa
     5521 atccaaaatg aatcgttgta cccattggca acgaaataaa atcaatagtc acaccataaa
     5581 aagcaactac taaggcagcg gcaatttgac cagccaattt tatatacggt ttcaaatcgt
     5641 agatatcatc tacaagccca agtacgtaca taataatcgc accaataata agtggtttga
     5701 tttctgtttc aataggatga ccaatccaaa tacctattaa aaatgaaaag agaatcactg
     5761 taccacccat aactgaaata ggtttagtgt gtacttttct aaaatttggt ttatcaacta
     5821 aatttaatct tttcgatatt gcaataacaa ttggtgttat cgtcaaactg acaatcattg
     5881 ttactgcaac tagtaataat gtaaccattt attcaccttc atcgatatta attgttttta
     5941 acg
//
LOCUS       DAMZLE010000135         5810 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMZLE010000135 DAMZLE010000000
VERSION     DAMZLE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.136"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..338)
                     /locus_tag="POB92_002254"
     CDS             complement(<1..338)
                     /locus_tag="POB92_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6579721.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKL"
     gene            complement(256..555)
                     /locus_tag="POB92_002255"
     CDS             complement(256..555)
                     /locus_tag="POB92_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDI6579722.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(542..988)
                     /locus_tag="POB92_002256"
     CDS             complement(542..988)
                     /locus_tag="POB92_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDI6579723.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(966..1277)
                     /locus_tag="POB92_002257"
     CDS             complement(966..1277)
                     /locus_tag="POB92_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6579724.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1291..2361)
                     /locus_tag="POB92_002258"
     CDS             complement(1291..2361)
                     /locus_tag="POB92_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDI6579725.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2333..3307)
                     /locus_tag="POB92_002259"
     CDS             complement(2333..3307)
                     /locus_tag="POB92_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDI6579726.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(3359..3982)
                     /locus_tag="POB92_002260"
     CDS             complement(3359..3982)
                     /locus_tag="POB92_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6579727.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESGKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3979..4308)
                     /locus_tag="POB92_002261"
     CDS             complement(3979..4308)
                     /locus_tag="POB92_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDI6579728.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4308..5294)
                     /locus_tag="POB92_002262"
     CDS             complement(4308..5294)
                     /locus_tag="POB92_002262"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDI6579729.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5291..5494)
                     /locus_tag="POB92_002263"
     CDS             complement(5291..5494)
                     /locus_tag="POB92_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDI6579730.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(5475..5807)
                     /locus_tag="POB92_002264"
     CDS             complement(5475..5807)
                     /locus_tag="POB92_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579731.1"
                     /translation="MTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGM
                     TEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNADYINLEKELT
                     KSNESKNK"
BASE COUNT         1881 a         1016 c          745 g         2168 t
ORIGIN      
        1 attaatttgt attcgatcat ttcttcacct ttatgtaaaa tgatacggtg ttgcctaatt
       61 tcaatatcgt ttctatctac tcctttaaaa tcatctagaa tatctcttga gaaaaattcg
      121 aaatctacag ttgttaaatc cctactttca attagaaaag ttttactaag tctaattaag
      181 tctggaacaa ttagtaaagt tatacttata accatcatcg ctactaacat ttcaatgagc
      241 gaaaaagctt tgacattaat actgtataca tgtcttttga taagaagtgg tattttttga
      301 aatagcacaa atttgttggt cactttgctt aatatcatac ttccctatag ttaccccctt
      361 cttaagttct tctttattaa ttttagatac agtcgtcaaa attactttag aagcatcaat
      421 tgtttgtagt ttatggttta tactcgcttg catttgattc atcattggta tcaatagtaa
      481 tgtaatcaat ccaattagca aaaatccagc catactatct attaagaatg aacctttaca
      541 cttatagctt ttcataacga attcttcctt tttcaatatg gaatattatt ctataaattg
      601 aattgttatt gtcaattgtt atgctaccaa atttattgat attccctttt ttatcaaagg
      661 caataatatc aacttttgca acattaatta ttttgcctac ttttaatttt agaaatcgta
      721 ttttattatt ctctattact ttaatagtgt cactgttttc ataaaatcta acattgatat
      781 atccttgatt tgctatagct tgcgacttaa tataattcaa ttcagtaata aaagaaatga
      841 tatttgcctc atcatctatt actctaagat tgcttaatcc tttagatgtc attgtcaaaa
      901 gtagaaatat actgattaac atcattacca caagcatctc aatcatagta aatgctgact
      961 gctttctaat ttgcaactgc ttctccatta ctaattgtga ttgtctctcc tgatttacat
     1021 gtcttttgtg cttcttttat aaaaccatct gcaattaagt cttcaataga cgatggattt
     1081 ctattatgtt tcaatgcata cgcttcaatt tgactattaa ccatttttac ctgtgcatta
     1141 caacctgttg attgtatgtg agcagtttgt ttagcaatat ttggaatgat taaaattaat
     1201 aataaactga tgattaataa cactaatagc atctctatca atgtaaacgc ttgagttttc
     1261 ttaagaaatt taaacatata ttaacctcca ttattttata ctttgcatca tttgaaacat
     1321 tggtaacata attactaaat aaattgcgac aataaataaa cctaaaatca aaaataaaat
     1381 aggctgtaaa aactgagtct gttttatcgc tttatcttct atttgtttta ctaatatttg
     1441 tgaatataac tttagttcta cttctagctt ccctctcttt tcaccttgta gcacaaactt
     1501 aattaattga ggcttaaagc attttagttt ttctaaaatt tgaggcaaac catatcccat
     1561 ttctgaataa gttaataagt atttacctag aaactgtcta aatggatcac tactatggtt
     1621 aatataaacg tctactattg attgaagtgt aataccattt ttataaaaca acactaattc
     1681 attagttaca aaataagttt taaataattg gaaatagcct gatattagcg gtagtttcat
     1741 cacaaagttt atcttattga gcatatttaa attgttataa attaatttca taataatagc
     1801 caacatagat actattatga gcattactac aattatagta ggtaagctgg taatgaaaaa
     1861 agacaatgtt ttttgaaaag aagatagttg aatattcata gaagtatata attgttgaaa
     1921 ctgtggaatt actgtgaggt ttaatataat aatcatagca ataaagatag aaactagtat
     1981 taaggggtat tgcagtgtct ttaacaatcg ttgttctgac tttctattca ctttcatata
     2041 atttacggtt tcttctagaa cgtctataat attgccaaat ctttctgcca aatatacttg
     2101 catgacgata gtatcgctat aacctatcag tgataatatt tgattgcatg gtgcaccatt
     2161 tgaaatttca cttagaatgg tggtacctaa ttgcttattt ttatatgtca tttgaagatt
     2221 taaaaattga aaactttgat acagagtgaa accatatttc aacaaattac ataaatttga
     2281 aagtaaatcg atttgttggg ccttacttaa ttgtcgcttc ttagaatgta gtttaaatgt
     2341 atttatccat tgtagtttca caaatgacac ctgcttttgt catatcatca agtttatctt
     2401 ctaagttctt aaatgatgat ggtaatgaat gattatggga aaagaaatat cggagttgtt
     2461 gctgagatag aatttcgcat actaattgtc gctgttgctt aatagtagtt acaagtcgtt
     2521 ggtttataat taagttagtt gcctgtatca attcttgtac agaaatgccc atttctaata
     2581 gccttaaaat agcaccttta caatcagttg catgcaatgt agtcagaaca aggtgaccac
     2641 ttaaactagc ctgtataaca cacttggcaa catctttatc tctgatttca cctattaaaa
     2701 taacatcagg atcacatctt aaaatagctt taaacgaatt tacatagtta atgccagctt
     2761 tatcattcac attaatttgg acgataccag gaatttgcat ctctacagga tcctctatag
     2821 aaattacatt taaattcaag gctttattcg cgtatgagac catttgatac attaatgtac
     2881 tctttcctga accagttggc ccactaaaca atagtaatcc ttgtttctta ttcatgaggt
     2941 gtttaaaatc attgaattta taagttgatt tttgttgttg aaaaaattga ggtacaattc
     3001 tgataacaca actttcttgg ccaagtgaca atggtaaagt tgatattctc aaaaaatata
     3061 ttttattgaa atggtaacta tatcgaccgc tctgtgcgac ttgctgtgta gaaacatcaa
     3121 gcccagcttg aaacttcata taaactaata acttttgata aatgctattc ccaatttgtt
     3181 catactgctc caagttatca ttaattctaa atttaatact tacttcattt ttaactggaa
     3241 taaaatgtac atcactcgct ttcatttcta tcgctttatt aattatttct tgaaatagaa
     3301 tcttcaaaaa aacacctcct acatataatc acgtaggagg tgtttttatt acttcaattt
     3361 aaccgtgtaa aaatggattt aattgttcat catcaaccgt cgtatatgga ccatgtccag
     3421 ggaataaagg taaatcgcct tctaattcaa atattttatc ttgaatagaa tcaactagcg
     3481 tttcataatc acctttatat aaatctgtac gtccgattcc attattaaat aatgtatctc
     3541 caacaactgc gaattcatcg aacacatatg ttaaacttcc tggtgaatgt ccaggtgtgt
     3601 gtaacacatt aaacttaaat ccttctattt ctgtgctacc ttcgtttaac ttttcaggag
     3661 ttaccttact tgtaataatt ggtaatccat attgcttaaa tttatctgcc ccatttttaa
     3721 cgggatcttt tagaaaatca aactctgctt catgcatata aaccgggaca tcgaatcgat
     3781 caactatatc atcgactgct ccgatatgat caaagtgtgc atgtgttaat aaaatagctt
     3841 ttaacggttt atttatttgg tttaattttt taataatttt tccactttca cctgaagggt
     3901 caatcagaat aacagctttg tcattttcga tgaaatacgt attagtatca actaagccta
     3961 aagttaagct tgaaatcctc ataggttttc accactattt tctaaatgtt tttgtactga
     4021 tgttagttta tcattcattt ttctagattt gtctcgtcgg tcatcaatac ggatatttgt
     4081 acaaactcta cttaaacctt tatcaaaagg taattcatgt atcacttgca caacttctaa
     4141 tacatcgctt aattcacctt caattagagt attcattggt gttaattgaa aatcaatttt
     4201 acccattgct ttatattctt gaagtttttt ctgaatatct gcaatatatt tactaacact
     4261 cggaccttcc gttccaactg gaataacaac cacatcaaca atagccatta ttttaccccc
     4321 tctttatcta atacataggt cttgattaat cctgctgctc ctgtaatacc tgcatcatta
     4381 cctaatttcg cttgtacaat ttcagtttca aattgagcag gtgcaaatgt taaattatga
     4441 tattctgttt taatattttc aattaaaata ggtcctgcag tagacattcc tccacctaga
     4501 acgatatatt tcggattact tgtaacacta ataatactac ataaatatcc aatatagttt
     4561 gcaacctttt cagtaatgaa aatacagaat tggtcaccag cttttgccgc atcaaaaaca
     4621 gcttttgctg taaccttatt ttctttaatc aattctaata tagaagatct aaacgtcaac
     4681 ttcggatagt agaagttaac taagttaaca acgcctgtcg ctgaagcaac tgtttcaata
     4741 catccagaac gaccacaatt acatttaaat cgttgatcga agtctgctct aaaatgacct
     4801 atttctgcgc cagagccatt atgaccatgt acgatttcac catttgaaat aattcctcca
     4861 cctagacctg taccaagtgt gatggcaaca acatcatcgg caccttcacc agcacctttg
     4921 tgtttctccc ctaaagcagc tatgttagca tcattatcta catacactgg acaatcaacg
     4981 aattgttcaa aaatctcacg tacattaact ttttctggcc aatataagtt tactgctcca
     5041 tttactgtac ctttttcaaa gtcaacagga cctggtacac caatacctac gccaagtaca
     5101 tttgaaaaat tataattatt ttcatttact ttttcaacaa acgaatcata aattcctttc
     5161 aaaagtgtat atcctgtact atcagatgta tcagtgtgaa tagaccattt atgtaattgt
     5221 tctaattcag gtgtgaaaat acctaattta caagtcgtcc cgcctacatc agctgctaaa
     5281 ataatttttg tcatttctgt tcattccttc tctgattaat tatcaacgta catttcaaat
     5341 attcttcctt agataacagt tcatattgat acaatgatga aatctcctgt tgaatcattt
     5401 cgtacatatc ttctggattt ttaaaatata ttagaaatcc gtaacttttc aataactgtt
     5461 gtacatcata aaagttattt atttttcgac tcatttgatt tagtcaactc tttttctaag
     5521 ttaatataat ctgcattttt agggtctgca tttaatgctt tacgcacata ttttaatgct
     5581 ttttcatcat catttaatga acgatttgct atcgctaact caaaatttaa taaacctgat
     5641 ttaggaaaca ttctaagtcc tcgctcccat tctgtcatac cttcggactt agaattgata
     5701 gtagccataa tcataccact taaataatat gtttgatcat cggcataatt tttatttatt
     5761 gtctgcttca caatattttg agcattatca taattaccac tagtcatatc
//
LOCUS       DAMZLE010000136         5795 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMZLE010000136 DAMZLE010000000
VERSION     DAMZLE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.137"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            165..1085
                     /gene="mvk"
                     /locus_tag="POB92_002265"
     CDS             165..1085
                     /gene="mvk"
                     /locus_tag="POB92_002265"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDI6579732.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1090..2073
                     /gene="mvaD"
                     /locus_tag="POB92_002266"
     CDS             1090..2073
                     /gene="mvaD"
                     /locus_tag="POB92_002266"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDI6579733.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2086..3162
                     /locus_tag="POB92_002267"
     CDS             2086..3162
                     /locus_tag="POB92_002267"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDI6579734.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHHCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3339..3680
                     /locus_tag="POB92_002268"
     CDS             3339..3680
                     /locus_tag="POB92_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDI6579735.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3732..5054)
                     /locus_tag="POB92_002269"
     CDS             complement(3732..5054)
                     /locus_tag="POB92_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDI6579736.1"
                     /translation="MKTYDLIVIGFGEAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIASKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIADSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNINYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGITL
                     HTNVETTELSSDNHHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGGVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(5041..5481)
                     /locus_tag="POB92_002270"
     CDS             complement(5041..5481)
                     /locus_tag="POB92_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI6579737.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLSNDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYEQDEQNIIINFYKGKTIKDVIEDIQKEDLCHENI"
     gene            5539..5640
                     /locus_tag="POB92_002271"
     CDS             5539..5640
                     /locus_tag="POB92_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579738.1"
                     /translation="MCQYLCLRTIELGVFIVMIYCLKVVALTVRLHN"
BASE COUNT         2001 a          858 c         1138 g         1798 t
ORIGIN      
        1 aaatataaat tcaaaataaa gattatacat aatatgaata atgtgagata aagactgcaa
       61 tgaaatttaa cgcgaatgcg tatgcaactt agaatatgat gtcttttact aataaataaa
      121 aataatgaat caagtatagg ataaatataa aaggtgatat tgacatgaca agaaaaggat
      181 atggggaatc gacaggtaag attattttaa taggagaaca tgctgttaca tttggagagc
      241 ctgctattgc agtaccgttt aacgcaggta aaatcaaagt tttaatagaa gccttagaga
      301 gcgggaacta ttcgtctatt aaaagcgatg tttacgatgg tatgttatat gatgcgcctg
      361 accatcttaa gtctttggtg aaccgttttg tagaattaaa taatattaca gagccgctag
      421 cagtaacgat ccaaacgaat ttaccaccat cacgtggatt aggatcgagt gcagctgtcg
      481 cggttgcttt tgttcgtgca agttatgatt ttttagggaa atcattaacg aaagaagaac
      541 tcattgaaaa ggctaattgg gcagagcaaa ttgcacatgg taaaccaagt ggtattgata
      601 cgcaaacgat tgtatcaggc aaaccagttt ggttccaaaa aggtcatgct gaaacgttga
      661 aaacgttaag tttagacggc tatatggttg ttatagatac tggtgtgaaa ggttcaacaa
      721 gacaagcagt agaagatgtt cataaacttt gtgaggaccc tcagtacatg tcacatgtaa
      781 aacatatcgg taagttagtt ttacgtgcga gtgatgtgat tgaacatcat aactttgaag
      841 ccttagcgga tatttttaat gaatgtcatg cggatttaaa ggcgttgaca gttagtcatg
      901 ataaaataga acaattaatg aaaattggta aagaaaatgg tgcgattgct ggaaaactta
      961 ctggcgctgg tcgtggtgga agtatgttat tgcttgccaa agatttacca acagcgaaaa
     1021 atattgtaaa agctgtagaa aaagctggtg cagcacatac ttggattgag aatttaggag
     1081 gttaatgcgt tgattaaaag tggcaaagca cgtgcacata cgaatattgc acttataaaa
     1141 tattggggta aaaaagatga agcactaatc attccaatga ataatagcat atctgttaca
     1201 ttagaaaaat tttacactga aacgaaagtc acttttaacg accagttaac acaggatcaa
     1261 ttttggttga atggtgaaaa ggttagtggc aaagaattag agaaaatttc aaaatatatg
     1321 gatattgtca gaaatagagc tggcatcgat tggtatgcag aaattgaaag cgacaatttt
     1381 gtaccaacag cagcagggtt ggcttcatcg gcaagcgcat atgcagcttt agcagcagct
     1441 tgtaatcaag cgctagacat gcagctgtca gataaggatt tatcgagatt ggcgcgaatt
     1501 ggttcgggtt ctgcgtcgcg tagtatttat ggtggatttg cagaatggga aaaagggtat
     1561 agtgatgaga cgtcatatgc cgttccactt gaatcgaatc attttgaaga tgaccttgcc
     1621 atgatatttg ttgtgattaa tcaacattct aaaaaggtac ctagtcgata tggtatgtca
     1681 ttgacacgaa acacatcaag gttttatcaa tattggttag atcatattga tgaagattta
     1741 gctgaagcaa aagcagcgat tcaagacaaa gattttaaac gccttggtga agtaattgaa
     1801 gaaaatggtt tgcgtatgca tgccacgaat ctaggatcaa caccgccgtt cacatatctt
     1861 gtgcaagaaa gttatgatgt catggcgctt gttcacgaat gccgagaagc ggggtatccg
     1921 tgttatttta caatggatgc gggacctaat gtgaaaatac ttgtagaaaa gaaaaacaag
     1981 caacagatta tagataaatt attaacacag tttgataata accaaattat tgatagtgac
     2041 attattgcca caggaattga aataattgag taaggaagag ataaaatgat tcaggtcaaa
     2101 gcacccggaa aactttatat tgctggagaa tatgctgtaa cagaaccagg atataaatct
     2161 gtacttattg cgttagatcg ttttgtaact gctactattg aagaagcaga ccaatataaa
     2221 ggtaccattc attcaaaagc attacatcat aacccagtta catttagtag agatgaagat
     2281 agtattgtca tttcagatcc acatgcagca aaacaattaa attatgtggt cacagctatt
     2341 gaaatatttg aacaatacgc gaaaagttgc gatatagcga tgaagcattt tcatctgact
     2401 attgatagta atttagatga ttcaaatggt cataaatatg gattaggttc aagtgcagca
     2461 gtacttgtgt cagttataaa agtattaaat gaattttatg atatgaagtt atctaattta
     2521 tacatttata aactagcagt gattgcaaat atgaagttac aaagtttaag ttcatgcgga
     2581 gatattgctg tgagtgtata tagtggatgg ttagcgtata gtacttttga tcatgaatgg
     2641 gttaagcatc aaattgaaga tactacggtt gaagaagttt taatcaaaaa ctggcctgga
     2701 ttgcacatcg aaccattgca agcacctgaa aatatggaag tacttatcgg ttggactggc
     2761 tcaccggcgt catcaccaca ctttgttagc gaagtgaaac gtttgaaatc agatccttca
     2821 ttttacggtg acttcttaga agattcacat cattgtgttg aaaaacttat tcatgctttt
     2881 aaaacaaata acattaaagg tgtgcaaaag atggtgcgtc agaatcgtac aattattcaa
     2941 cgtatggata aagaagctac agttgatata gaaactgaaa agctaaaata tttgtgtgat
     3001 attgctgaaa agtatcacgg cgcatctaaa acatcaggcg ctggtggtgg agactgtggt
     3061 attacaatta tcaataaaga tgtagataaa gaaaaaattt atgatgaatg gacaaaacat
     3121 ggtattaaac cattaaaatt taatatttat catgggcaat aaatgatttg ataggggagc
     3181 aatcatctaa tttaattaga agataaatgc tccatttttt attgccagcc gtgttacgta
     3241 tcttgaaaaa aatattaaaa agagtaaaat agatagagtt aacgaaaaaa ttaatgaaat
     3301 cgacgatata gatatgataa agaaaggtgg atagcaatat gaaaaataca ttccttattt
     3361 gtgatgaatg tcaggcagtc aatataagaa cgttacaaaa gaagttggaa aaattagatc
     3421 ccgatgctga aatcgtgata ggttgtcaat cttattgtgg acctggacgc cgaaaaacat
     3481 tcacttttgt taataaccgc ccactggctg cgcttactga agaagaatta atcgaaaaag
     3541 tttctcaaca attaaagaaa ccacgtgatc ctgaagaaga agagcgttta agaaaacgac
     3601 atgaagaacg taaacgtcgt aaagaagaac aagatagaaa gcttaaagaa aaattagaaa
     3661 agcgaaaagc acaacaataa agcctgatgg cagcatcatt caatgcgtgc caccaggttt
     3721 ttatgttttg tctagaaatt aaataaatca ttaaatgatt cggccatcgt aggatgcgta
     3781 taaatattat ctcgtaatac ggtatatgga atgttttgat caatcgcaag tttaattata
     3841 ttaattaatt cttcagattg cttaccatat aatgtagcac ctaaaatcat attattttca
     3901 ttattaatga ctactttaaa taaacctctt ggatcattgt taattttgtg acgaggtata
     3961 gcacttacta aaagttgatg ttcagtgtaa tcataatgtt gagcggcagc ttctttacta
     4021 gttaatccaa cacgtgataa tggtggatct ataaatactg tataaggcac gccacctcta
     4081 ttgtcagtcg tacgtgactg attaccatat aacgctgatt tgataattcg ataatcgtct
     4141 aaagatatat acgtaaattg aagtccgcct ttaacatcac ctgcagcata aatatgcggt
     4201 acagttgttt gaagatgagc attgacttta atttcgcctc tgtcgcctaa ttcgatatca
     4261 gtattttcta aagctaaatc cgtattcggt ttgcgcccga tagccaaaag tactgcatca
     4321 gcctcaaagt taccaacgtt ggtatggact gttgtatgat gattgtcaga tgacaattca
     4381 gtcgtttcaa catttgtatg caatgtaatg cctttatttt ctaagtcagt aatagcatgt
     4441 gcaacgacat cttgatcttc acgtggcata aatgattcac cacgttctaa tactgtaacc
     4501 ttactaccta aattcgcaaa cattgaagca aattctaagg cgatataacc gccacctaca
     4561 ataacgaggt gcttaggttg atagttaatg tttaataaac ctgttgaatc gaagacgtgt
     4621 ttagcttgat caaggccttt aatgttagga ataacagagg tagcaccagt attaataatg
     4681 atatgaggtg cagtaatact atcggcgata tcgtcatgct gatctaataa attcacttca
     4741 gtattagatt taaactgcgc tttaaaatcc agtacatcaa tgttgttatc gtctgctaat
     4801 aaatggtaat ttttattgtt tagcgcattg acaacatcgt ttttacggtt ataacttgct
     4861 tcaaaagatt tgccttctaa tccatcatgt acaagtgtct ttgaagcaat acatcctatg
     4921 tttatacaag tgcctccata cattttcgga gattgttcga taactgcgac gtgttgacct
     4981 gttgatgcag catatttggc taaagtttta ccagcttccc caaatcctat tacaattaaa
     5041 tcatatgttt tcatgacata aatcctcctt ttgaatgtct tcaatgacat ctttgattgt
     5101 ttttccttta taaaaattaa taatgatatt ctgttcgtct tgctcataat gtgacatagt
     5161 agttgcaata ttacgagcaa tttgacagtg actgccttcg tcgccagtaa atagtcgtgt
     5221 gtgatgttct ttctctaaga caaaatgttt atataatgtt gctagagaga catcagcgct
     5281 acgatcattt gataaataac caccatcttt accccgtatt gtgttaatca tttttaaatc
     5341 gacaagttga gtcgtcacgc gtcgtaattg aacaggattt aaacaagtta attctgctaa
     5401 tgaactacta ttgaattttt ctgaatgatg cttagttaaa aaagctaata catgcacggc
     5461 aatgttaaat tctaaattca atgtttctca cctcataatt gtaactatat tagttacaat
     5521 taaaaatcat tttgctcaat gtgtcaatac ttatgtttga gaactattga attaggtgtg
     5581 tttattgtga tgatttattg tttgaaagtt gttgcattaa cggtgagatt gcacaattga
     5641 aattttagtt atgtataaac tgatagatat gaaatgaata ttgaaaataa gggcaataaa
     5701 attttgaatg atgatgttgt gtgattgtat aaacttattc gtattttaga tacgttttga
     5761 tatttcgcat gatgaggaaa tattttcaac aactg
//
LOCUS       DAMZLE010000137         5786 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMZLE010000137 DAMZLE010000000
VERSION     DAMZLE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.138"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            11..991
                     /locus_tag="POB92_002272"
     CDS             11..991
                     /locus_tag="POB92_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6579739.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            954..1646
                     /locus_tag="POB92_002273"
     CDS             954..1646
                     /locus_tag="POB92_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDI6579740.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            1856..3022
                     /locus_tag="POB92_002274"
     CDS             1856..3022
                     /locus_tag="POB92_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6579741.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGKVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVAQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3003..4241
                     /locus_tag="POB92_002275"
     CDS             3003..4241
                     /locus_tag="POB92_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDI6579742.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFV
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            4231..5661
                     /locus_tag="POB92_002276"
     CDS             4231..5661
                     /locus_tag="POB92_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDI6579743.1"
                     /translation="MKSDSLKENIIYQGLYELIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLSNNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRLLRHKTI"
BASE COUNT         1879 a          787 c         1055 g         2065 t
ORIGIN      
        1 tataaatgag gtggaagatt tggaaagagt tttgataact ggtggggctg gttttattgg
       61 gtcgcattta gtagatgatt tacaacaaga ttatgatgtt tatgttctag ataactatag
      121 aacaggtaaa cgagaaaata ttaaaagttt ggctgacgat catgtgtttg aattagatat
      181 tcgtgaatat gatgcagttg aacaaatcat gaagacatat caatttgatt atgttattca
      241 tttagcagca ttagttagtg ttgctgagtc ggttgagaaa cctatcttat ctcaagaaat
      301 aaacgtcgta gcaacattaa gattgttaga aatcattaaa aaatataata gtcatataaa
      361 acgttttatc tttgcttcgt cagcagctgt ttatggtgat cttcctgatt tgcctaaaag
      421 tgatcaatca ttaatcttac cattatcacc atatgcaata gataaatatt acggcgaacg
      481 gacgacatta aattattgtt cgttatataa cataccaaca gcggttgtta aattttttaa
      541 tgtatttggg ccaagacagg atcctaagtc acaatattca ggtgtgattt caaagatgtt
      601 cgattcattt gagcataaca agccatttac attttttggt gacggactgc aaactagaga
      661 ttttgtatat gtatatgatg ttgttcaatc tgtacgctta attatggaac acaaagatgc
      721 aattggacac ggttataaca ttggtacagg cacttttact aatttattag aggtttatcg
      781 tattattggt gaattatatg gaaaatcagt cgagcatgaa tttaaagaag cacgaaaagg
      841 agatattaag cattcttatg cagatatttc taacttaaag gcattaggat ttgttcctaa
      901 atatacagta gaaacaggtt taaaggatta ctttaatttt gaggtagata atattgaaga
      961 agttacagct aaagaagtgg aaatgtcgtg aaaatgacat tgaagctgtc cataataata
     1021 agggttatgc ctatcaaaga aaattagaca aactagaaga agtgagaaaa agctattacc
     1081 caattaaacg tgcgattgac ttaattttaa gcattgtttt attattttta actttaccga
     1141 ttatggttat attcgccatt gctatcgtca tagattcgcc aggaaaccct atttatagtc
     1201 aggttagagt tgggaagatg ggtaaattaa ttaaaatata caaattacgt tcgatgtgca
     1261 aaaacgcaga gaaaaacggt gcgcaatggg ctgataaaga tgatgatcgt ataacaaatg
     1321 tcgggaagtt tattcgtaaa acacgcattg atgaattacc acaactaatt aatgttgtta
     1381 aaggggaaat gagttttatt ggaccacgcc cggaacgtcc ggaatttgta gaattattta
     1441 gttcagaagt gataggtttc gagcaaagat gtcttgttac accagggtta acaggacttg
     1501 cgcaaattca aggtggatat gacttaacac cgcaacaaaa actgaaatat gacatgaaat
     1561 atatacataa aggtagttta atgatggaac tatatatatc aattagaaca ttgatggttg
     1621 ttattacagg ggaaggctca aggtagtctt aatttactta ataagttcaa ataaaagtta
     1681 tattttaaag attgtgacca attgttacag tataacgagg aatcccttga gacagtatca
     1741 aatggcatta agaaatatgt gccatcattg atttgcatgg ctataaatac tattcatctg
     1801 atgagatagc catgttaaga aattgaaagt atagcattaa aggggtttgt aacagttgaa
     1861 aattatatat tgtattacta aagcagacaa tggtggtgca caaacacatc tcattcaact
     1921 tgccaaccat ttttgcgtac accatgatgt ttatgtcatt gtaggcaatc atggaccaat
     1981 gattgaacaa ctagatgcaa gagttaatgt aattattatc gaacatttag taggtccaat
     2041 tgactttaaa caagatattt tagctgtcaa agtgttagcg cagttattct cgaaaattaa
     2101 acctgatgtt atccatttac attcttccaa agctggaacg gtcggacgaa ttgcgaagtt
     2161 catttcgaaa tcgaaagaca cacgtatagt ttttactgca catggatggg cttttacaga
     2221 gggtgttaaa ccagctaaaa aatttctata tttagttatc gaaaaattaa tgtcacttat
     2281 tacagatagc attatttgtg tttcagattt cgataaacag ttagcgttaa aatatcgatt
     2341 taatcgattg aaattaacca caatacataa tggtattgca gatgttcccg ctgttaagca
     2401 aacgctaaaa agccaatcac ataacaatat tggcaaagta gttggaatgt tgcctaataa
     2461 acaagattta cagattaatg ccccgacaaa gcatcaattt gttatgattg caagatttgc
     2521 ttatccaaaa ttgccacaaa atctaatcgc ggcaatagag atattgaaat tacataacag
     2581 taatcatgcg cattttacat ttataggcga tggacctaca ttaaatgatt gtcagcaaca
     2641 agttgcacaa gctgggttag aaaatgatgt cacatttttg ggcaatgtca ttaatgcgag
     2701 tcatttatta tcacaatacg atacgtttat tttaataagt aagcatgaag gtttgccaat
     2761 tagcattata gaagctatgg ctacaggttt gcctgttata gccagtcatg ttggcggtat
     2821 ttcagaatta gtagctgata atggtatatg tatgatgaac aatcaaccag aaactattgc
     2881 caaagtcttg gaaaaatatt taatagacag tgattacatc aaaatgagta atcaatctag
     2941 aaaacgttat ttagaatgtt ttactgagga gaaaatgatt aaagaagtgg aagacgttta
     3001 taatggaaaa tcaacacaat agtaaattac taacattgtt acttatcggt ttagcggttt
     3061 ttattcagca atcttcggtt attgccggtg tgaatgtttc tatagctgac tttatcacat
     3121 tactaatatt agtttattta ctgtttttcg ctaaccattt attaaaggca aatcattttt
     3181 tacagttttt cattattttg tatacatatc gtatgattat tacgctttgt ttgctatttt
     3241 ttgatgattt gatatttatt acggttaagg aagttcttgc atctacagtt aaatatgcat
     3301 ttgtagtcat ttatttctat ttagggatga tcatctttaa gttaggtaat agcaaaaaag
     3361 tgatcgttac ctcttatatt ataagcagtg tgactatagg tctattttgt attatagctg
     3421 gtttgaacaa gtccccttta ctaatgaaat tgttatattt tgatgaaata cgttcaaaag
     3481 gattaatgaa tgaccctaac tatttcgcga tgacacagat tattacattg gtacttgctt
     3541 acaagtatat tcataattac atattcaagg tccttgcatg tggtatttta ctatggtctt
     3601 taactacaac ggggtctaag actgcgttta tcatattaat cgtcttagcc atttatttct
     3661 ttattaaaaa gttatttagc agaaatgcgg taagtgttgt gagtatgtta gtgattatgc
     3721 tgatattact ttgttttact ttttataata tcaactacta tttattccaa ttaagcgacc
     3781 ttgatgcctt accgtcatta gatcgaatgg cgtctatttt tgaagagggg tttgcatcat
     3841 taaatgatag tggatctgag cgaagtgttg tatggataaa tgccatttca gtaattaaat
     3901 atacattagg ttttggtgtt ggcttagtgg attatgtaca tattggctcg caaattaatg
     3961 gtattttact tgttgcccat aatacatatt tgcaaatttt tgtggaatgg ggcattttat
     4021 tcggtgcgtt atttatcata tttatgcttt atttactgtt tgaattattt agatttaaca
     4081 tttctgggaa aaatgtaaca gcaattgttg taatgttgac gatgctgatt tactttttaa
     4141 cagtatcatt taataactca agatatgtcg cttttatttt aggaattatc gtctttattg
     4201 ttcaatatga aaagatggaa agggatcgta atgaagagtg attcactaaa agaaaatatt
     4261 atttatcaag ggctatacga attgattaga acgatgacac cactgattac aatacccatt
     4321 atttcacgtg catttggtcc cagtggtgtg ggtattgttt cattttcttt caatatcgtg
     4381 caatactttt tgatgattgc aagtgttggc gttcagttat attttaatag agttatcgcg
     4441 aagtccgtta acgacaaacg gcaattgtca cagcagtttt gggatatctt tgtcagtaaa
     4501 ttatttttag cgttaacagt ttttgcgatg tatatggtcg taattactat atttattgat
     4561 gattactatc ttattttcct actacaagga atctatatta taggtgcagc actcgatatt
     4621 tcatggtttt atgctggaac tgaaaagttt aaaattccta gcctcagtaa tattgttgcg
     4681 tctggtattg tattaagtgt agttgttatc tttgtcaaag atcaatcaga tttatcattg
     4741 tatgtattta ctattgctat tgtgacggta ttaaaccaat tacctttgtt tatctattta
     4801 aaacgataca ttagctttgt ttcggttaat tggatacacg tctggcaatt gtttcgttcg
     4861 tcattagcat acttattacc aaatggacag ctcaacttat atactagtat ttcttgcgtt
     4921 gttcttggtt tagtaggcac ataccaacaa gttggtatct tttctaacgc atttaatatt
     4981 ttaacggtcg caatcataat gattaataca tttgatcttg taatgattcc gcgtattacc
     5041 aaaatgtcta tccagcaatc acatagttta actaaaacgt tatctaataa tatgaatatt
     5101 caattgatat taacaatacc tatggtcttt ggtttaattg caattatgcc atcattttat
     5161 ttatggttct ttggtgagga attcgcatca actgtcccat tgatgaccat tttagcgata
     5221 cttgtattaa tcattccttt aaatatgttg ataagcaggc aatatttatt aatagtgaat
     5281 aaaataaggt tatataatgc gtcaattact attggtgcgg taatgaattt agtattatgt
     5341 cttgttttga tatattttta tggaatttac ggtgcggcta ttgcacgttt aattacagag
     5401 tttatcttac ttatttggcg atttgttgat attactaaaa tcaatgtgaa gttgaatatt
     5461 gtaagtacga ttcaatgtgt cattgctgcc gttatgatgt ttattgtgct tggtgtggtc
     5521 aatcattatt tgccccctac aatgtacgct acgctgctat taattgcgat tggtatagta
     5581 gtttatcttt tattaatgat gactatgaaa aatcaatacg tatggcaaat attgaggctt
     5641 cttcgacata aaacaattta agtatcggta acgctatact ttagaaattt aagattaaga
     5701 agaaaaggca atttcttatt gaaaaatgga agttgtcttt tttaattctc tttaaaagcg
     5761 ggaaacaaaa gcagttaaat gccttt
//
LOCUS       DAMZLE010000138         5778 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMZLE010000138 DAMZLE010000000
VERSION     DAMZLE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.139"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..450
                     /locus_tag="POB92_002277"
     CDS             <1..450
                     /locus_tag="POB92_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6579744.1"
                     /translation="IIFNNVDLKNLSLLQLLTGLKANIWFLIIFVLTVLVFNLLIVKF
                     IIWLINETRKFMNLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAG
                     SDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            complement(447..593)
                     /locus_tag="POB92_002278"
     CDS             complement(447..593)
                     /locus_tag="POB92_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579745.1"
                     /translation="MNGGENFMADKNKKQEATRSNPINKSFEKPGASENLKSTLSEKA
                     KKKD"
     gene            complement(629..811)
                     /locus_tag="POB92_002279"
     CDS             complement(629..811)
                     /locus_tag="POB92_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579746.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(1011..1781)
                     /locus_tag="POB92_002280"
     CDS             complement(1011..1781)
                     /locus_tag="POB92_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6579747.1"
                     /translation="MREKVSNRLKHIMKIRNLKQVDIINKSKPYQKKLGISLSKSTLS
                     QYINDVQSPDQDRIYLLSKTLNVGEAWLMGYDVDSYRVPDEERQDETIMSKINNIFSQ
                     LTPPRQENVLNYANEQLEEQNKVTSIDGYKESKLVSYIACGATGAGIGEELYDDILHE
                     EVFFKEDETPSNADFCILVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLI
                     KRVDICEDYINLVSLNPKYDDIKVASFSDIKVMGKVVL"
     gene            1940..2164
                     /locus_tag="POB92_002281"
     CDS             1940..2164
                     /locus_tag="POB92_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HDI6579748.1"
                     /translation="MCYDYSRLSGKIVEKYGTQYNFAIAMKLSERSLSLKLNGKVGWK
                     DSEIWKAIQLLDIPVEKIHLYFFKEKVHVI"
     gene            2182..2442
                     /locus_tag="POB92_002282"
     CDS             2182..2442
                     /locus_tag="POB92_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6579749.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDEKINATPIKE"
     gene            complement(2466..3005)
                     /locus_tag="POB92_002283"
     CDS             complement(2466..3005)
                     /locus_tag="POB92_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579750.1"
                     /translation="MDIIAICIAIFSFLLTALKYYLDYMKDSLNIDVIPTRSFNYLVD
                     DKSSYNDITFINFTKFPISVIDVEFDIKNKVNEQKTFKPIRYKDKNYSIPFTLGPYES
                     VECTFLLEEYPVIWEWDVTIKVTTNKGIYIKPVIIESRTEHRESEPQVTELTSANKVS
                     ALSNPKDGFLKKFLYHLKP"
     gene            3062..3811
                     /locus_tag="POB92_002284"
     CDS             3062..3811
                     /locus_tag="POB92_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6579751.1"
                     /translation="MQALKTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHS
                     DGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            3827..4024
                     /locus_tag="POB92_002285"
     CDS             3827..4024
                     /locus_tag="POB92_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579752.1"
                     /translation="MQAQNKKVIYYYYDEEGNRRPVNIQYNDGYDLMIDPRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            4055..4195
                     /locus_tag="POB92_002286"
     CDS             4055..4195
                     /locus_tag="POB92_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579753.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERSNNPELLRAVAELLKK
                     VN"
     gene            complement(4210..4842)
                     /locus_tag="POB92_002287"
     CDS             complement(4210..4842)
                     /locus_tag="POB92_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579754.1"
                     /translation="MYIDPLKNVRFSINNVISNVEISKSMAIKQSLKPKYQLDIINRN
                     NINLFSDFKVDFHLNNLIEMNFNLRNSFSSLTFQRNLFSEETIKSFKELYRFDDEIVL
                     QAQQTIRDFYINPTAISTLAEAINSTYPINEQSTYKRHDEFVKRIENDFPHPFKKLIR
                     WSNGIAAGADIQIFVTNYINENDLHIQNSLIVAIVCLLSFLSTYCSHSKK"
     gene            4901..5221
                     /locus_tag="POB92_002288"
     CDS             4901..5221
                     /locus_tag="POB92_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDI6579755.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            5218..5379
                     /locus_tag="POB92_002289"
     CDS             5218..5379
                     /locus_tag="POB92_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDI6579756.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            5472..5732
                     /locus_tag="POB92_002290"
     CDS             5472..5732
                     /locus_tag="POB92_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6579757.1"
                     /translation="MYYKIGDVCQKVINVDGFDFKLAVKKQDYSILVNVLDLEDRFID
                     GINITDENDLYTALDILNQSIYEWIEENTDERDRLINLVMRW"
BASE COUNT         2090 a          832 c          955 g         1901 t
ORIGIN      
        1 ataattttta ataatgttga tttaaaaaat ttgagtttac ttcagttgct tacaggtcta
       61 aaagcaaata tttggtttct aattattttt gttttaacag tgcttgtatt taatctttta
      121 attgttaaat tcattatctg gttaattaat gaaacaagaa agtttatgaa tttggattgt
      181 ataagcttat tagacaaaag agacaagttg tttaataaca acggtaaacc agtatttata
      241 gttattaaag actttgaaaa cagaatcatt gaagagggtg aacttaaaac ctataattca
      301 gctggtagcg atttcgattt actagaggtt gagcgacaag atttcaaagt atctgattta
      361 ccgtcaaacg atgaattgta tattaaacat acacttgtag accttaaaca acaaattaaa
      421 ttggatttat atttaatgaa tgaatattaa tcttttttct tagctttttc tgataaagtg
      481 ctttttaagt tttcgctggc acccggcttt tcaaaacttt tgtttattgg gttactacga
      541 gtagcttctt gttttttgtt tttatccgcc ataaaattct caccaccatt caacgtctac
      601 acttgtaggc gttttttgat tagtaaaatc ataatgaatc ttctttggtt aacttatcgc
      661 catctatttt ttgtgaaata aattccaagt atttacgcgc attatgtgac gataaatctt
      721 taggtaactc ataagtgaat ggttgattac cactagttaa aacttcatat actatagttt
      781 ctttttttat tttgcaatta gttattttca ttataaactt cctttcaaac actgctgaaa
      841 tagacgtctt tttcaaataa gcataattaa tactttaatt ctttaatcca catatattta
      901 aaagtgaggt agtaggtaat aaatataaga cttaaagtta agattgcttt tttcatgtca
      961 atttctcctt tgtttatatt tatattaaag tgctaaatat acgttattaa tcacaataca
     1021 actttgccca ttactttaat atcactaaac gaagcgactt tgatatcatc atacttcgga
     1081 tttagagata ccaaattaat atagtcttcg catatatcta cacgcttgat aagacttact
     1141 ccatctaata caacgagtgc aattgtacca tctttaatag aatcttcttt cttaataaaa
     1201 gcgtatgttc cttgttttaa cataggttcc attgaatcac cattaactaa aatacaaaaa
     1261 tcagcatttg atggcgtttc gtcttcttta aaaaatactt cttcatgcaa tatgtcatca
     1321 tataattctt ctcctatgcc agcaccagtt gcaccacatg caatatacga tactagttta
     1381 gactctttat atccatctat agaagtgact ttattctgtt cttccaattg ttcatttgca
     1441 tagttaagta cgttttcttg gcggggaggt gtgagttgag aaaatatgtt attgattttt
     1501 gacattatcg tttcatcttg acgttcttca tcaggaactc gataagaatc tacatcatac
     1561 cccataagcc acgcttcacc aacgttcaga gttttagaaa gtaggtaaat tctatcttgg
     1621 tcgggtgatt gtacgtcgtt aatatattga gataaagtgc ttttacttaa agatatacct
     1681 agtttctttt gataaggttt cgatttatta atgatatcta cttgttttaa gtttcttatt
     1741 ttcataatgt gtttaagtct atttgaaact ttttctctca tttagtgcac ctccgtttga
     1801 taacttcata ataaagcttg ttgaacaaaa attcaacaaa aaagttcata aatcatgaat
     1861 ttttgtattg acttgattca aaacaaggtg taaagtatag ttaagttcat gatacgtgaa
     1921 cttgagagga ggtgctttta tgtgttacga ctactcacgt ttgagcggga aaatagtaga
     1981 aaagtatggc actcagtaca attttgcaat tgctatgaaa ttgtcagaga gaagtttatc
     2041 cttaaaactc aacggtaaag ttggttggaa agacagtgaa atatggaaag ctatacaact
     2101 actagatata ccggtagaga aaatacactt atattttttt aaagaaaaag ttcatgttat
     2161 atgaacttaa ggaggggcac aatggaacaa atcacgttaa ccaaagaaga gtgtgtcgaa
     2221 caatgcatca ataaagactt aaaactttta gattatcgag ttcaacaaat tttagaaggt
     2281 gttctatcag aaagtaccac atacggtgat gcaagaaata aattagaaac attgaaaatt
     2341 attgctgaat ctcattttaa aaccgaacat gcttcagtta tttacaaatt agcattgaaa
     2401 aagttagacg aaaaaatcaa cgccactcca attaaagagt gacggaaagg gaggatttta
     2461 aatgtttaag gttttaaatg atataaaaac ttctttaaaa aaccatcctt ggggttggaa
     2521 agagcactta ccttatttgc tgatgttaac tctgtcactt gtggctctga ttctcggtgt
     2581 tctgtccgcg attctatgat aacaggcttt atatagattc ctttgttggt agtgactttg
     2641 atagtcacat cccattccca tatcactgga tattcttcga gcaaaaaagt acattctaca
     2701 ctttcataag gtcctaaagt aaatggaatg gagtagtttt tatctttata tcgtataggt
     2761 ttgaacgttt tttgttcatt tactttattt ttaatatcaa attcaacgtc aataacagaa
     2821 atgggaaact ttgtgaaatt aataaatgtt atatcgttgt aacttgattt gtcatcgacc
     2881 aagtaattaa agcttctggt aggtataaca tcgatgttaa gagaatcttt catatagtct
     2941 aaataatatt taagtgcagt cagtaagaaa ctaaaaattg cgatacaaat cgcgattatg
     3001 tccatactta tcacctcctt aggttgataa caacattata cacgaaagga gcataaacaa
     3061 tatgcaagca ttaaaaacaa aatcgaacat cggcgaaatg ttcaacatac aagaaaaaga
     3121 aaatggagaa atcgcaataa gtgcaagaga gttatataaa gctttggaag ttaaaaagcg
     3181 ttttagcgct tgggcagaaa ttaacttgaa gcatttcaaa gaaaataggg attttacaag
     3241 tgtacttaca agtacggttg ttaataacgg agctgtaaga caactagaag attatgcttt
     3301 aacacttgat gtagctaaac atgttgcgat gatgtcaggt acagaaaaag gttttgattt
     3361 tagagagtat ttcatccaag tagagaaagc atggaacagt ccagaaatga ttatgcaacg
     3421 tgctttaaaa attgctaaca acacaatcaa tcaattagaa acaaagattg aacgtgataa
     3481 accaaaaatt gtatttgcag atgcagtagc tactactaag acatcaattt tagttggaga
     3541 gttagcaaag atcattaaac aaaacggtat aaacatcggg caacgcagat tgtttgagtg
     3601 gttacgtcaa aacggattcc ttattaaacg caagggtgtg gattataaca tgcctacaca
     3661 gtattcaatg gaacgtgagt tattcgaaat taaagaaaca tcaatcacac attcggacgg
     3721 tcacacatca attagtaaga cgccaaaagt aacaggcaaa ggacaacaat actttgttaa
     3781 taagttttta ggagaaaaac aaacatctta ataggaggaa cgaacaatgc aagctcaaaa
     3841 caaaaaagtc atctattact actatgacga agaaggtaat agacgacccg ttaatattca
     3901 atacaacgat ggctacgact taatgataga cccgcgtttt attgaaatga cgcttgaaag
     3961 acatccgcat ttaaaaaata acttttatgg attaatagat ggaaaagaat ttaagttaga
     4021 ttaaattttt ggaaatgcaa aggaggcata acaaatgtta caaaaattta gaatcgcgaa
     4081 agaaaaaaat aaattaaaac tcaaattact aaagcatgct agttactgtt tagaaagaag
     4141 taacaaccct gaattgttgc gagcagttgc agagttgtta aagaaggtta actaaattag
     4201 gccttattat tactttttag aatgtgaaca ataggtcgat aaaaaactta ataaacaaac
     4261 tatagcaact atcaatgaat tttgaatatg taaatcgttc tcgtttatat agtttgttac
     4321 aaagatttga atgtcagcac ctgctgcaat gccattagac catcttatta actttttgaa
     4381 aggatgtgga aaatcatttt cgatacgttt gacaaattca tcgtgtctct tgtaggtact
     4441 ttgctcattt attggatagg tcgaattgat ggcttcagcc aaagtagaga tagcagttgg
     4501 attgatataa aaatctctaa tggtctgttg tgcttgaagt acaatctcat catcaaacct
     4561 atagagttcc ttaaaagatt ttatcgtttc ttcagaaaat aaatttcttt gaaatgttag
     4621 agatgaaaaa gaattacgca aattaaaatt catttcaatt aagttgttta gatgaaagtc
     4681 tactttgaag tcagaaaata aatttatgtt gtttctatta attatatcta attggtactt
     4741 aggttttaaa gattgtttaa ttgccatact tttagaaatt tcaacattac taattacgtt
     4801 attaatagaa aaacgaacat tttttaaagg atcaatatac accaatatca cctcctttca
     4861 ctaggagata acaacattat acacgaaagg aaagatagaa atgccacata ttttaaacgt
     4921 aacagttcca atacctgaaa cacatgtact tatcacaaaa gatgaatatg atgagctaat
     4981 tggttattca ttagaccctg tatggaacat gagtgactta aagaagaaat taaaaattgc
     5041 atctgatgag actatcaagg acagattact atttcatcct agatttgaaa aagaactaag
     5101 agcgcaagga attgtgcatt accctgatga gaattttaat cgctggagat ttaacgcaag
     5161 aaagatgaat aaattcgtcg atgagcattt caatgaaata tataaggaga gaataaaatg
     5221 agcaacattt ataaaagcta cctagtagca gtactgtgct ttacagtctt agcaattgta
     5281 cttatgccgt ttctatactt cactactgca tggtcgattg cgggattcgc aagtatcgca
     5341 acattcatat tttataagga atacttttat gaagaataaa aaaactgtta ctcacggcaa
     5401 tgagtaacag tctaaacaat tagaaaatta atgcatattc aatataaaac gaaataaagg
     5461 aagtgtcaac aatgtactac aaaattggcg atgtatgtca aaaagtaatt aatgtagacg
     5521 gattcgattt taaattagca gttaagaaac aagattacag cattctagtg aatgtcttag
     5581 atttagaaga tagatttatc gacggtataa atataacaga tgagaatgat ctatacacag
     5641 cattagacat attaaatcaa tctatttatg aatggattga agagaacaca gacgaaagag
     5701 acaggctaat taacttagtc atgagatggt aggtataagc atgagagata cagaaagaaa
     5761 tatattgaat atttttaa
//
LOCUS       DAMZLE010000139         5768 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMZLE010000139 DAMZLE010000000
VERSION     DAMZLE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.140"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..314
                     /gene="fmtA"
                     /locus_tag="POB92_002291"
     CDS             <1..314
                     /gene="fmtA"
                     /locus_tag="POB92_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDI6579758.1"
                     /translation="MGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFYAKPT
                     LNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIV
                     Q"
     gene            complement(851..1129)
                     /gene="qoxD"
                     /locus_tag="POB92_002292"
     CDS             complement(851..1129)
                     /gene="qoxD"
                     /locus_tag="POB92_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDI6579759.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(1138..1743)
                     /gene="qoxC"
                     /locus_tag="POB92_002293"
     CDS             complement(1138..1743)
                     /gene="qoxC"
                     /locus_tag="POB92_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDI6579760.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(1733..3721)
                     /gene="qoxB"
                     /locus_tag="POB92_002294"
     CDS             complement(1733..3721)
                     /gene="qoxB"
                     /locus_tag="POB92_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDI6579761.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(3721..4821)
                     /gene="qoxA"
                     /locus_tag="POB92_002295"
     CDS             complement(3721..4821)
                     /gene="qoxA"
                     /locus_tag="POB92_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDI6579762.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            5123..5245
                     /locus_tag="POB92_002296"
     CDS             5123..5245
                     /locus_tag="POB92_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579763.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(5393..5710)
                     /locus_tag="POB92_002297"
     CDS             complement(5393..5710)
                     /locus_tag="POB92_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDI6579764.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         1996 a          935 c         1001 g         1836 t
ORIGIN      
        1 atatgggtaa attaattact caaatacaac aatataaatt attcagtcct aaaataacca
       61 atccattatt acatgagttt ggtacgaaac agtatccaga tgaatatcga tatggtttct
      121 atgctaagcc aacattgaat agacttaacg ggggattctt tggacaagtc tttactgttt
      181 actataatga taagtatgta gttgtacttg cattaaatgt aaaaggaaac aatgaagttc
      241 gaatcaaaca tatttataat gatattttaa aacaaaataa accttacaat acgaagggtg
      301 ttattgttca ataattaata tagaagatat aacatgtata tggcattaag gcatcgacct
      361 tatctgacca gtatacgagt tatatcttct tttttatagt ggtaaaaagt ttaaagtata
      421 aggttgaaga aggatgagtt taaaaatatg tgttaactga taaaagggga aatcatttgg
      481 tgagttggca tcagactaaa atgaatgaag acgaatacgt tggtccatgc gtggtgtgaa
      541 tgttttctaa atctaactgg aaatgataat ttaataatgt acacttttga ttgtttaaac
      601 atgtacaatt tttaaaaacc tagggataat tttattgacg tgaacaacat tgagattatc
      661 tttagacata aatatggatt ttgaagttac tatatttatt agttgtggtt gttaatgtat
      721 taattgaatt gattataatg attgtcttat aagatataga aggggactta accatttaaa
      781 aattgaagga aaataaaaac cagctaacaa aagtgttagc tggttaaact ttacattatt
      841 aaaatgttgt ttataagtgt gaagagtgac cgccttgcat aacccaatat gttccgacaa
      901 cgaaacaaag tgtaattaca agagcaaaga taactttgaa tgtttgtaaa cgtccatctt
      961 taccttcagt taaatgcatg aacattaata attgaagtcc tgcttggacg aatgcaaagc
     1021 caaagataat tgtcaacttc gcgtggaatg ttaatgacgt gtatagtgtt acgtatactg
     1081 ctaaaagcgt taatacgata gatgcgataa atcctacagt atgtttcatt attgtactca
     1141 tccgctatac accatcccta tcatatatac ggcagtaaag atgaaaaccc aaacaacatc
     1201 taagaagtgc cagtataaac ttactataaa taattttggc gcattatatt tgtctaatcc
     1261 gcgtcgttgg atttggatta ataaacaaat ggcccaaacg atacctagcg atacgtgaca
     1321 accatgcgtt cctaatagga taaagaaact agaccagtaa gaaccaattg ttgggttaac
     1381 gccttctgat gcatagtgtg cgaattcata aatttcgaat ccaacaaaga ctaaacctaa
     1441 aagtaacgta atgatcatcc aaaacatcat taacttttgt ttttcttggc gcatgtagta
     1501 aatagcaata ccacatgtgt aagaactgaa taataatgca aacgtcatta ttaaaacaag
     1561 aggcaattca aataactcag tagtcatttt acctgcataa tcgccaccat gttgcaaagt
     1621 taatagtgtc gcaaataggg taccgaataa cgcaaattcg gctgtaatga aaatccaaaa
     1681 gccaagctta tttaattcgc cttcatgtgt gcgtgaatca atagtgtttg tatcatgact
     1741 catgacttac agcctccctt tctttaatac gcgcttctct taatctagct tcagtttcag
     1801 caacttcagc agcagggata tgatatccgt gatcgatttg gaaactgcga taaatcatag
     1861 taccaaaaat accgaataaa caaattaatg ctggaattac agtttcgaaa attaagaaga
     1921 aaccgccgat agtcataaag ataccaatcc agaatccaac aggagtattg tttggcatat
     1981 gaatgtcttt gtaattatgg ttgtctaagt aatgacgacc atgttctttc atatcaacaa
     2041 atgtgtcgta gtcattccaa tctggtgtaa tggcaaagtt gtatttaggt ggaattgctg
     2101 atgcagtagt ccactctaga gtacgaccaa ggccatccca gttatctcca gttgcttcac
     2161 gtggagattt gaagtgactg tatacgatac taacaacaag gaataagaat ccgattgcca
     2221 ttaataatgc accgatagtt gaaattaagt ttaataagaa ccaaccatct gatggcatat
     2281 aagtgtataa acgacgtggc ataccatcta atccaagaat gaattgtggt aagaaacaaa
     2341 cgttaaatcc gatcatgaag aaccagaagc accatttgtt taatgtttcg tttaatttgt
     2401 aacccatcat ctttggatac cagaagatta aaccagctaa gcaggcaaat acaacaccag
     2461 taaccaatgt atagtggaag tgagctacta agaagtacgt attgtgatat tgatagtcag
     2521 ctgatgccat tgctaacatt acacccgtaa caccacctaa aaggaagtta gggataaatg
     2581 ctaatgagaa tagcattggt gactcaaatg taatacgtcc tttatataat gttaataacc
     2641 agttaaacaa tttcacacca gttggaatac caatcagcat tgttgaaatt gagaagaatg
     2701 agttgattaa cgcaccatta cccattgtga agaaatggtg aacccaaact aagaaactaa
     2761 ggaacgcgat accggcagtt gcccatacca tactttgatg tccgaataaa cgcttacgag
     2821 cgaatgtcgg gataatttct gagtaaatac caaatgctgg aaggataacg atataaactt
     2881 cagggtgccc ccatacccag aagaagttag cccaaagcat tggcataccg ccatgtgcaa
     2941 ctgtgaagaa tgctgtgtca aatattctat cagttgtcat taatgctaac gctactgtta
     3001 aaggagggaa agcaagaata acaattaatg tagtaataaa tgttgttact gtaaacattg
     3061 gcatttgcat aaacttcata gttggtgttt tacatcttaa aattgttaca aagaagttga
     3121 tacctgtagc taaggtacca agccctgaaa tttgtatagc tattaagtaa tagttaacac
     3181 ccggaccagg actgaattca cctgctagtg gcgcatagtt tgtccaacca gctgctggtg
     3241 aaccaccaat aataaatgac aggttgaata aaatcatacc tgcaaagaat agccagaaac
     3301 ttacgttgtt taatactggg aatgcaacat cacgtgctcc aatttgtaat ggaacaacga
     3361 tattccataa accaaagata aatggcattg ccatgaagat aatcatgatt acaccatgtg
     3421 tactaaaaat ttcgttatag tggttagatt ctaaaaattt gttatcaggt actgttaatt
     3481 gcgcacgaat aagtaacgca tcaataccac cacggacgaa cattaatacg gcacagatta
     3541 aatacataat accgattttc ttatggtcta cagatgtgaa ccattctttg taaagatatt
     3601 tccataattt aaagtaagta attactgcga ttaaaccaat aactaagaat ggggcaccaa
     3661 tttgtgccat tgtaatcatc cagttacctt taactagtaa ttgatcccat ggaaaattca
     3721 ttaatgtcca cctccatgat catcattgtc ttgatcttgc gcatcttttg aaattttctt
     3781 catttctttc gcatttttcg attcatcttt cttgaactca ttgttatatg gttcgtcatt
     3841 tccaagaatc atcaacttca taccatgtcg tttatagttc gcatttgtaa tttgagcttt
     3901 acgagcaggt attaatggtt tgtctgatac atctttaaac atattttctt cactagtgaa
     3961 gtttggatct ttcaattcga aattgaaacg tttatatgca tagaagatgt attctggatc
     4021 ggctgctgga tcaacaaacg ccatatgtgt accattaaat tctaaagctt tattaggtgt
     4081 gcttggtaat aattgtttat caaatgtatc ttgatctaac gttttcttac ctttaacttc
     4141 tttcacccat ttgtcgtagt ctttttgact aacggcattt actttaaatg tttgacgtga
     4201 gaatccttca ccattgaagt tagagttacg acctctgaac gtaccagttt gagatgcttc
     4261 taacgtccaa ttcattgtca tgccagtcat ggcatatttt tgaccaccta attgtggaat
     4321 ccagaaactt gtcattgtat ccatagcttg aagcttaaat acaacaggac gatctttagg
     4381 gattgttaat gtgttaacag tctctatatg ttcatctgga taagcaaaga accatttgta
     4441 tcctgcactt actgcatata caaccattgg atctttctca ctcttcggtg gtttttcgta
     4501 atcgtataaa gttttaactg taggaatagc taaagcagca acgattatga taggtattac
     4561 aaaccatatt gtttcaatga tggcattatg gtgcatctta ccagattcgg cattcttatt
     4621 ataactatac ttgtaaataa aaatggcgaa catgccaagt acaacgaaac aaataacaag
     4681 catgaagacg attgaataaa gaatcaagaa cttctgacta cttgctactg gcccttttgc
     4741 gttgaaaatt tctatatttg aacaaccact aagtaaaatt agtgtgccaa ataatagaag
     4801 caaagactta aattttgaca cttttttgac ctcctaatac tacaaatgta gggcttaaca
     4861 ttaattttaa gttattacac aatatttaca agggcttatg ggaaaaaaat taataaaatt
     4921 gtatcaaaaa tgttgataaa tcaaggtgtg acgtgggttc acacatttgt taaaattatg
     4981 tgtacatttt gtgactaata gcgtttttga aaaaattgct tcatatttaa aaggaaagtt
     5041 gagaatagct ttcaattaaa ggttaattga atgaaaataa aaaaagtttg aaattgtcta
     5101 ataaattgaa aatgatggct agttgattga aaggaagcgc aaacatttta ataaggaatt
     5161 tgccaaaagt aatttatcgt taaacaatga aaagtggaaa tttgtttgtt caaaatcaaa
     5221 acattttttt aaaaagagca gttaagtttt aataatttga taggtagtcc tttggtatac
     5281 agaaaaaata caatatttgg gttaaagttg cagatgaagt tagaataaca aataaaatgg
     5341 tagcaaaaca tgttcgtttg ctaccatttt atttgcagta tagtgatgag ggctatttaa
     5401 cctcaatata cctagtggaa attttaattg caccatctga atcagtgaca tgatatttaa
     5461 tttgatattt accagattta gatgtatcaa tttggccatc gactttaatt ttatcggtta
     5521 aatctccatc ttctttatca aatgcactta tgccgtttag aaggttataa tcttgacctt
     5581 tcttaataac gatatcatta gcgcctttaa tttgtggtgt agtattcgtc gttgcatctg
     5641 catttaaatt tggtgttact agtgtagcag aaacaccgag ggctgataat gactgtaata
     5701 gtttattcat aatgtaacct ccgaatgata agtcatttct gagttagctt tactatacct
     5761 taatttat
//
LOCUS       DAMZLE010000140         5712 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMZLE010000140 DAMZLE010000000
VERSION     DAMZLE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.141"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            419..805
                     /locus_tag="POB92_002298"
     CDS             419..805
                     /locus_tag="POB92_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI6579765.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            798..1094
                     /locus_tag="POB92_002299"
     CDS             798..1094
                     /locus_tag="POB92_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6579766.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      1152..1255
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1344..2369
                     /locus_tag="POB92_002300"
     CDS             1344..2369
                     /locus_tag="POB92_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDI6579767.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2362..3057
                     /locus_tag="POB92_002301"
     CDS             2362..3057
                     /locus_tag="POB92_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579768.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            3075..3896
                     /locus_tag="POB92_002302"
     CDS             3075..3896
                     /locus_tag="POB92_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6579769.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKVKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            4250..4444
                     /locus_tag="POB92_002303"
     CDS             4250..4444
                     /locus_tag="POB92_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6579770.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(4677..5528)
                     /locus_tag="POB92_002304"
     CDS             complement(4677..5528)
                     /locus_tag="POB92_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDI6579771.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGFYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT         2094 a          798 c         1008 g         1812 t
ORIGIN      
        1 ttcataagcc acagctaatg tgtatttaaa aataggaata catgagtaaa actcatgcat
       61 aagaaatact aatttctaaa gaaaaagtat ttctttatgt tggggccccc caacttgcac
      121 attattgtaa gctgactttc cgccagcttc tgtgttgggg ccccgctata attgaaaagt
      181 ttgttatagg tgtattttct tttggttaac tattgttaat ataacattgt agattttagg
      241 atgttgattt ttgacttagc ctttgtctgc ttatgttaaa tgttccagct agtcaaacat
      301 tgagtttgta attattatat taagctagtt taaatttttt gtgatttatg ttttattcat
      361 accaattttg tgaaatgata cagtgacaga tgtttattga ttttaaaggg tgatgatgat
      421 ggctattgta aataaagtga taattgttga aggaaaatct gataaaaaaa gggtgcaaca
      481 ggttattgca gaaccagtca atattatttg tactcatgga acaatgagta tagataagct
      541 tgatgatatg atagaatcac tgtatgataa acaagttttt gtattagccg attctgatga
      601 cgaaggagat cgaattagaa attggtttaa acgttatttg agtgaaagtg aacatatatt
      661 tattgataaa acttactgtc aagttgcgaa ttgccccaaa caatatttgg cgcatgtact
      721 ttcaaaacat ggctttactt gtaagaaaga aacacctctt ttaccgaata taaataatga
      781 aaggttagtt ttagtaaatg aataattcat tagacatcaa agatgtaact acattttatg
      841 aggaagacaa acatttaatc tttggttata caccaacgtg tggtacttgt aaggtttcag
      901 aaagaatgtt agacattgct aatgaaatat tgcagttacc attattgaaa atagatttaa
      961 acttttatcc tcagttttgt aaagatatgc aaatcatgtc tacgccgatt ttattgttga
     1021 tgaataaaga taaagaagta aaacgaattt atgcatttaa atcggtgact gatttgttag
     1081 aaaatttaaa atagttattg acgaaatttt actaaggtgt taggattaat acaaattaaa
     1141 tgatgcgtaa actcttatcg agagtggtgg agggatgtgc cctacgaagc ccggcaaccg
     1201 tcttatatag aaatggtgcc aattcacata aagttttaac ttttgaagat gagagaaaca
     1261 atactactat tgctttctca atttttctat cgatattgag aaagcatttt ttattttatt
     1321 aagcaacaca gggaggaatc aacgtgattg aattaaaaga agttgttaaa gaatatcgga
     1381 ctaaaaataa agaagtcctt gctgtagatc acgttaattt atcgattcga gcaggatcga
     1441 tttatggcgt cattggtttt tctggagcag gaaaaagtac tttgattcga atgtttaatc
     1501 atttagaagc gcctacatca ggtgaagtta ttatagatgg agaccatata ggtcaattgt
     1561 ccaaaaatgg attaagagca aaaagacaaa aagtaagtat gatcttccaa cattttaatt
     1621 tgttatggtc aaggactgtg ttaaaaaata ttatgtttcc gcttgaaatt gcaggtgtcc
     1681 ctagaaggag agctaagcaa aaagcattag aacttgtcga actcgtcggt ttaaaaggta
     1741 gagaaaaggc ttatccatca gagttatcag gtggacaaaa gcaacgtgtt gggattgcac
     1801 gagcgttagc taatgatcca acggtcttgc tttgtgatga ggcaacaagt gcacttgatc
     1861 cgcaaacaac agatgaaatt ttagatctac tactaaaaat tagagaacaa caaaatttaa
     1921 caattgtact aattacgcat gaaatgcatg tcattcgtcg tatttgtgat gaagttgcag
     1981 ttatggaaag tggtaaagtg atagaacaag gaccggtgac acaggttttt gaaaatccgc
     2041 aacacactgt gacaaaacga tttgtgaaag acgatttaaa tgatgatttc gaaacatctt
     2101 taacagaatt agagccatta gaaaaagatg catatatcgt tagattagtt ttcgctggtt
     2161 caacaacaac cgagcctatt gtatcgagtc tatcaactgc ctatgatatt aaaattaata
     2221 ttttagaagc aaatattaaa aatacaaaaa atggaacagt cggcttttta gttctgcata
     2281 ttccatatat ttcaagtgta gatttcggaa aattcgaaaa agagttaatt gagcgacaag
     2341 ttaaaatgga ggtgttaaga catgggtaaa tcatttagtg aaattataaa tgaaatgatt
     2401 acaatgccta atattcagtg gccagaagtt tggactgcaa tagtcgaaac actatacatg
     2461 acagtcgtct caactatatt tgcatttata cttggtctta ttttaggtgt gttattattc
     2521 ttgtctgcta aaggtaagtc tatcggtgca aggttatttt attctatcgt ttctttcatt
     2581 gttaacttat ttagagcgat accatttatt attttaattt tattattaat tccatttaca
     2641 agtttgatac ttggaacgat aagtggtccg acaggtgcgt taccagcctt gatcattggc
     2701 gcagcaccgt tttatgcaag gctcgtagaa attgctttta aagaaattga taaaggtgtc
     2761 atcgaagcgg cttggtcaat gggcgctaat acttggacag taattcgtaa agtcctttta
     2821 cctgaagcta tgccagcgct agtgtctggc attacagtta cagcaatcgc tttagttggt
     2881 tcaacagcag ttgcaggtgt aattggtgcc ggtggtttag gaaatttagc atacttaaca
     2941 ggtttcactc gaaatcaaaa tgatgtcatt ttagtatcaa cagtttttat tttaattatt
     3001 gtatttataa tccaattcat tggggattgg cttacaaata aacttgataa acgataaatt
     3061 gggggtttca ttttatgaaa aaattatttg gtcttatttt agtattaaca tttgcagttg
     3121 tattagcagc ttgcggtaat ggaaacaaaa gtggcagtga cgacaagaaa ataacagtag
     3181 gtgcttcacc agcaccacat gctgaaattt tagaaaaagc aaaaccatta ttagagaaaa
     3241 aaggttatga actagatatt aaaacaatta acgattacac tacacctaat aaattactag
     3301 acaaaggtga aattgacgca aactatttcc aacatacacc atatttaaac acagagaaaa
     3361 aggataaagg ttacaaaatc gtaagtgccg gtgatgttca cttagaacct atggctgtat
     3421 actctaaaaa gtataaaagt ttaaaagaat taccaaaagg tgcaacagtc tatgtgtcta
     3481 ataatccagc tgaacaagga cgtttcttaa aattcttcgt tgatgcaggt ttaattaaag
     3541 tcaaaaaagg cgtaaaaatt gaagatgcta agtttagtga tattacagag aataaaaaag
     3601 atattaagtt taataataaa caatcagcag aattcttacc taaaatttat caaaatgaag
     3661 acgctgatgc tgttatcatt aattcgaact ttgcaatcga acaaaaacta aatcctaaaa
     3721 aagattctat tgctgtagaa agtgcgaaag ataatcctta tgcaaactta attgctgtta
     3781 aagaaggaca tcaagatgat aagaaaatca aagcattaat tgaagtatta caatctaaag
     3841 atattcaaga cttcattaat gaaaaataca atggtgcagt tattcctgct aaataaatca
     3901 tattaatggc tcagtaacgt gatgttgctg ggctttttaa tttaaacagg tatttatatg
     3961 atatttagga atgagatgct attacggata aagtaaaatc ctatatgcat gcgtgctact
     4021 catttattct tatttttaat ttcacatggc tagtgactaa tacatcataa atcattagtg
     4081 attctttatt atttcagtcc cactccctca agttaatgat gaatctattt cgctagttat
     4141 aaaatgacgt caaatcaata tcaaaaatta tttaagtaaa atgtttagat aatttttcag
     4201 tgggtaagta ttatatataa catttaatta ttccgaggag gcatttatta tggcagacga
     4261 aagtaaattt gaacaagcaa aaggtaatgt taaagaaaca gtaggtaatg ttactgataa
     4321 taaaaattta gaaaacgaag gtaaagaaga taaagcttct ggtaaagcga aagaattcgt
     4381 tgaaaatgca aaagaaaaag caactgattt tattgataaa gtaaaaggta acaaaggcga
     4441 gtaattttac tttttattcc taatacactg cacatgccac ttagtcggta taaccatcgg
     4501 ctaggtggtt tttgtattaa aaaagtggat accaaaattt attaataatt attttaatgt
     4561 tagaaaaaaa ctaaataaaa actcgctaat gatatccaat aatatgtata caaaacgaga
     4621 catatattgc atatgattaa cgagatactg aaaatatttt atcaccccta aaatgattat
     4681 tcattttcag cggtaattcg acctaaagtc aaacttacaa taaaaccgat gataaatact
     4741 actaatgaaa cgaaccacat cacgatatta gttggtaaac ctggaaatac tgcaaagagg
     4801 gagccaacaa caaaaccaat gattaatgca aaagtcatta gtttatgatg tgttaggaaa
     4861 tactggataa ttttgcttga aataatgaat ccagcaagca cgccaaatcc gactgcaagt
     4921 aatataggaa gacctgcaaa gttaagttta acaacttcag atattgctag catgaccgta
     4981 ccatagaagc caaatactaa taacataaat gaccctgaaa tacctgggag taacatagca
     5041 ctagatgcac acatacctgc aataaaatat ttaataataa gactagttga tagagtaagt
     5101 gtttctccag catgtttatc accattattc attaatgtaa taacaattaa gatagcgata
     5161 ccagctataa ccatcatgta atgtttagtt gtaaatgacg ttttatagtt agaaattttc
     5221 aataaatatg gaacgatacc aatgattaat ccaccaaaga aaaacatagt tggaatatgg
     5281 tgttggctta ataaataatt aaaaagatta cttagtgatc ccattgccag taacattcca
     5341 attataatgg ggattaaaaa tgtaaaactt ggccaaaaac gtcgtgagaa tatgccgcta
     5401 attgaagcga taaattgatt gtaaatacct aacaataatg cgatagtccc accgctaaca
     5461 ccaggtacca agtcactcgt tcccatagca aaacctttta gaatattaat ccatttaaat
     5521 tgttgcatga ataactcctt tcaaacgatt ggaataaaat cataaatagc atcataccat
     5581 attacaaatg tcctagtgaa atgataacat attttaaatt cataaaatcc attgagaaat
     5641 tatgtgcact tattatcatt tatattttta aagagagcgg attagagttg tactttagag
     5701 ttattaaaaa ta
//
LOCUS       DAMZLE010000141         5653 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMZLE010000141 DAMZLE010000000
VERSION     DAMZLE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.143"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..489)
                     /locus_tag="POB92_002305"
     CDS             complement(<1..489)
                     /locus_tag="POB92_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579772.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LIN"
     gene            complement(888..1310)
                     /locus_tag="POB92_002306"
     CDS             complement(888..1310)
                     /locus_tag="POB92_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDI6579773.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVSPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            1458..2174
                     /locus_tag="POB92_002307"
     CDS             1458..2174
                     /locus_tag="POB92_002307"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI6579774.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFINDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            2257..2796
                     /locus_tag="POB92_002308"
     CDS             2257..2796
                     /locus_tag="POB92_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDI6579775.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLSGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(2946..3266)
                     /locus_tag="POB92_002309"
     CDS             complement(2946..3266)
                     /locus_tag="POB92_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6579776.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYKDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            3410..3766
                     /locus_tag="POB92_002310"
     CDS             3410..3766
                     /locus_tag="POB92_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDI6579777.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3924..4304
                     /gene="gcvH"
                     /locus_tag="POB92_002311"
     CDS             3924..4304
                     /gene="gcvH"
                     /locus_tag="POB92_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDI6579778.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            4483..5361
                     /locus_tag="POB92_002312"
     CDS             4483..5361
                     /locus_tag="POB92_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDI6579779.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMVLKEMK
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDNLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT         1998 a          793 c          987 g         1875 t
ORIGIN      
        1 attaatcaaa tcataaaaaa gccaatgttc atcgaaatag gtatttccga ttttaattgc
       61 atttttctca aaaccaagaa tgtcgaatcc tagtgcacta tagaaaactt tagcacctat
      121 gttgtttgac acaattgatg taagtaaaga ttcgtaattg ttctgccgtg cataattaat
      181 aatgaaatta atgagctcgc tattaatcga tttatcgtta ttagtaacaa agttgtattt
      241 aattaatgat ttatgttctt ttccaacgta tcgaatttgt tctaaggcag ctgttgcgat
      301 aagtgtttcg ttttcataac agccaaatac aatacatttc ggtgaacctt gatttaaaat
      361 gtcatgtatt aaggtgtcag tcattgtttg actataatgt gaatcttgag tgaattcttc
      421 attgactgtc gaaacaagtc tcttatactc attaaaatca ttattcgtta atgttttaat
      481 ttctatcatt actgacctcc ttaatcatta ttaagaaaga ttttgtacta aaaaaatttc
      541 ttaattacat attaaaatgt ttattaaaat tttgcaatca ctgatttata tgcacattct
      601 tgtaaatgga ttatcaattt aattatattt atttttaagt tcaggaaatt atggaattgg
      661 ttattggtcg acactggact tttatttgta gacggtatgc gaagaagtca agccagtatt
      721 ggaaccggtc acatttatga tgactaaggg acaatcattt tcagttaaat attgccagaa
      781 atttcagtca cgaatagaaa aaataaccaa ccaaaaattt ttggttggca aatttgaatt
      841 ttatgtgaat tttattaata acgttgaata atctctttaa gttaatgcta atctacaaca
      901 tttacattta aatcgacatt aatatttcct tgagtcgctt ttgaatatgg acaaaattca
      961 tgagccattt gtaaatattt ttcagcttct tcttgagata taacattttt aattgtcgca
     1021 tcaattgaaa cacttaattt aggactttct gagtctgaat catcttctag tctcactgtt
     1081 agtgttactt ctggatgagc atcacgcact ttgttttgct ttaaaattag gtcgaaagca
     1141 ccgttgaagc aagatgcata acctgctgca aataattgtt cggggttagt agctttacca
     1201 tctgcttgag caggcgaaac gatatcaata tctaacgctc gatcatcagt ataaacatgt
     1261 cctttacgtc cgccaacatt cgtcgcttta gtttcataat gtattgccat tcttataacc
     1321 tcctaattta aaatacattt atgctttacc cattcgaaaa taccttaatc attttcattt
     1381 atagcattgt ttgattgaag gataaaaagt tgttgtttac aataaaaata atgagtatct
     1441 gaaatgaggg atttactatg acacatgtgg aagtagtagc gactatcgcg ccacaattat
     1501 ctatcgaaga aactttaatt caaaaaatta atcatcgtat tgatgcaata gacgtattag
     1561 aattacgaat tgatcaaatt gaaaatgtca cagttgatca agtggcagaa atgattacaa
     1621 agctgaaggt tatgcaagat tcattcaaat tattagttac gtatcgtaca aagttacaag
     1681 gtggctatgg gcaatttata aatgacttgt atcttaattt aatatcagac ttagcaaata
     1741 tcaatggcat agatatgatt gatatagaat ggcaagcaga tattgacatt gaaaaacatc
     1801 aacgaatcat tacacacttg caacagtata ataaagaggt ggttatatca catcataatt
     1861 tcgaaagtac gcctccatta gatgaattgc aatttatatt ttttaaaatg caaaaattca
     1921 acccagaata cgttaaatta gcagtaatgc cacataataa aaatgatgtg ttaaatttat
     1981 tgcaggcaat gtctacattt tcagatacta tggactgcaa agttgttggt atttcaatgt
     2041 ctaaacttgg actaataagt agaacggctc aaggcgtttt tggtggtgca ttgacttatg
     2101 gttgtatcgg agaaccacaa gctccaggac agattgatgt tactgattta aaagcacaag
     2161 tgactttata ctaataaaat gaattaggtt gcttttgtgt aagtgctgtc ttataattga
     2221 gaagtattat cattgttcgt ggaggagcga ttttagatgg aattacaaca agcaatagct
     2281 aatagaagaa gtgtgaaaaa atttaaaaga gatatgcaca tagatgacgc attgctatat
     2341 caagcaattg agaaagctgc tgatgctcca aatcacggaa tgagggaacc atggagagtt
     2401 gtgcatgttc cgaaagacag attaggagat atgagtaagg atatttctaa atttgcattt
     2461 cctaatgaat tagataagca acaatgtcat tatgatgcag ttacgaaact aggtggcatg
     2521 ttattgctta ttttaaaaac agatccaaga caacgtcaaa atgatgaaaa ctactttgca
     2581 tttggtgcat atgcacaaaa tcttatgttg ttactttatg aagcgggaat aggtacatgt
     2641 tggaaatcgc cattatatat ctatgatcct aaagtaagaa aaacacttgg tataaagaaa
     2701 gatgaagttc tttctggatt cttatattta acggatttag aagaagatat gcctaaagca
     2761 ccacgtaaaa atagaaactt aattacatta tattaatatg tataattata gaaacattaa
     2821 taaaagctga gtcatgaatt gatggacatc tatcgagtta gagatttaat ctaacttact
     2881 agagtcggta caatcacagt ctcagctttt tattgtgcag tatatacaca tttttatttt
     2941 agtatttatt taaaagtttc tgctaaaaat gattcaactt gctcaggtga cttagcattt
     3001 gctgaatgaa ggtgtgcaat tttgtcgccg tttttaaata ctagcaagct agggataccc
     3061 ataacttcat tttcaacaac tacatcttct aattcatcac gattaacagt ataccattgg
     3121 taatctttat attgttctac gattgggtca atccataaat ccatagcacg acagtctggg
     3181 caccatcctg cctcaaattt aacaattaca ggtgtatcgc tattaatcac agatttaaat
     3241 gattcattac ttttgattgc ttgcattgta acaactcctc tagatagttt aataattttt
     3301 attatagcta aatttataac ataataaaaa attttagctt caaaatgaaa gccttttgct
     3361 atggaaaatg atatatttat tttaaataca taaaggaggt tgcagtcgta tgattaaatt
     3421 ttaccaatat aagaattgta caacttgtaa aaaggcagca aagtttttag atgaatatgg
     3481 cgtaagttat gaaccaattg atatcgttca acatacacct acaataaatg aatttaaaac
     3541 aataattgca aatacaggcg tagaaattaa taaattgttt aatacacacg gcgcgaaata
     3601 tcgtgagctt gatttgaaaa ataaattaca aactttatca gatgatgaaa agttagagtt
     3661 gttatcatct gatggtatgt tagtaaagcg tcctctagca gtaatgggcg ataagataac
     3721 gttaggattt aaagaagatc aatataaaga gacttggtta gcgtaagtat aatgtaaacg
     3781 tttactaaat atctcgatat ttagattcat tacatgtaaa atgaaataag ctatacaatt
     3841 gttaattttt ataaatatag ttgaatagca tctagcctta tggcatcatt aatgatgtaa
     3901 agattaatta ggaggggatt ctcttggcag taccaaatga attgaaatat tcaaaagagc
     3961 atgaatgggt taaagttgaa ggtaatgtag caacaattgg aatcacagaa tacgcacaaa
     4021 gcgagttagg tgatattgtt ttcgttgaat taccagaaac agatgatgaa attaatgaag
     4081 gggatacgtt tggtagcgta gaatcagtta aaactgtatc agaattatat gcaccaatct
     4141 ctggtaaagt agttgaagtc aacgaagaac tagaagatag tcccgaattt gtaaatgaat
     4201 ctccatacga aaaagcatgg atggtaaaag tagaaattag tgatgaaagt cagattgaag
     4261 ctttattaac agctgaaaaa tattcagaaa tgattggtga ataatcaccg tgtaactcct
     4321 taatctaaga ttgaggagtt tatttttagt ctgagtaagt aaataatatg aaagaaaatt
     4381 tattaggtac tattatttgg agtattgcta cattttatta ttcaagaatg atgaaaataa
     4441 tgaatttagc tattttaaaa ataaaaattg ggggaagtta atatgctaaa cattcaagac
     4501 gttagtcatc tttctaaaaa ggagcaaaaa gcatataacc gtttcgtaga atctgtagaa
     4561 aacggtaatt taccagtact accatgtatt gaaatggttc taaaagagat gaaagaagaa
     4621 acattaaacc agagtaagat tggtggaatg ccatttttaa aatcttttaa agatatacca
     4681 ttagatgaaa ataatgtacc aatggtattg ttagcacaga ttaatttgga taatcttcca
     4741 gaacaacaag aattatttcc tgtaaaagaa gggatattgc agttttggat tagttcagaa
     4801 gatcaaatgt atggtatgtc tgaaaattta aagggaaaca atataaactc aaggcttgtt
     4861 tatataaaag agccaattac agatttatca ctcgaaaata ttcaagcgca tttgaagtca
     4921 ttagatgctg ataatgagga tatcccgttc agtggagcat tttctataga atttagattg
     4981 tcgaaacaaa ctattacatg tactgattat aagtacgatg aggacgtgct tgcattgtgg
     5041 aataaagtca atccatcctt cgcgctaaaa tcaatgtttg gtggttatga tgaattgatg
     5101 gaacctgtgt gtaacacatt tactgctaag gaaccattta atcaacttgg tggttatcca
     5161 tattttgacc aaatagatcc aagaacgaac gatcaagaac tgaaaatgta tgatagagtc
     5221 ttactgcaaa ttgattctac aagagatggt aattcttcga ttatatgggg tgatttaggt
     5281 attgccaata tcttagtaaa atctactgac cttgaggcta tgaagtttga tgattacatg
     5341 tattcatggg attgcagcta agagaatcat aaaaatatat tactttaata agtgtagtat
     5401 ctatagatca atctagggag tgggacagaa atgatatttt cgcaaaattt atttcgtcgt
     5461 cccaccccaa cttgcattgt ctgtagaaat tgagaatcca atttctcttt gttggggccc
     5521 atccccaact tgcattgcct gtagaatttc ttttcgaaat tctctgtgtt ggggcccctg
     5581 actagaattg aaaaaagctt gttacaagcg cattttcgtt cagtcaacta ctgccaatat
     5641 aacttcgtag agc
//
LOCUS       DAMZLE010000142         5432 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMZLE010000142 DAMZLE010000000
VERSION     DAMZLE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.144"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            110..796
                     /locus_tag="POB92_002313"
     CDS             110..796
                     /locus_tag="POB92_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDI6579780.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRLLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            786..1445
                     /gene="nth"
                     /locus_tag="POB92_002314"
     CDS             786..1445
                     /gene="nth"
                     /locus_tag="POB92_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDI6579781.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNSSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            1450..1791
                     /locus_tag="POB92_002315"
     CDS             1450..1791
                     /locus_tag="POB92_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579782.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(2355..4538)
                     /locus_tag="POB92_002316"
     CDS             complement(2355..4538)
                     /locus_tag="POB92_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6579783.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(4535..5212)
                     /gene="recU"
                     /locus_tag="POB92_002317"
     CDS             complement(4535..5212)
                     /gene="recU"
                     /locus_tag="POB92_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDI6579784.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1839 a          850 c          900 g         1843 t
ORIGIN      
        1 atttaaaatt tgtttgctac tagttaaata tattaagcat tttagtcgaa aattaaagaa
       61 aatgatttat actattaagt aatggttaaa tttaaaaggg ggcacgaaca tggataaata
      121 tcaattaaaa gcaagacctg tagtgatacg aagagaatta ttagaccatt acagtgactt
      181 aggtttagat gagcaagatt tagtcatttt gcttaaattg atttatgctt ccgaaacatc
      241 aaataaacag ccttcaattg aattacttca gaagggctca actatgcaac cacgcgatat
      301 tacaatggtt attcaaaatt tgatacaacg tgaattgcta gaattacaag ttcaaaaaga
      361 tgaagaaggt agatttaccg aatatatgaa tctagatcct tttttcgaaa aattaagtca
      421 tatattgaag caacaaagta tggaaacgaa agagcaaaat agtaaagaaa aatttaaaca
      481 attatttaga cttttagagg atacatttgc aaggccgtta tctccatatg aaatagaaac
      541 gttaaatcaa tggattgatg ttgataaaca cgatagtgca attatacaag cggcattaga
      601 cgaggcgaat agtttgaata aacttagctt taaatatatg gatcgtattt tacttaattg
      661 gaagaaaaac aatgtcaaaa caattgacga ttctagaaaa ataagagaaa aatttaataa
      721 accgaaaatg acgcacacag ttaaaacggt acctaaattt gattggttaa acggggagaa
      781 ccttgatggt aagtaagaaa aaagcattag aaatgattga tgttatagca aatatgtttc
      841 cggatgcaga atgtgaatta aaacatgata atccgttcga attaactatt gctgtattat
      901 tgtcagcgca atgtacagac gttctagtaa atagagtaac aactgagctg tttaaaaaat
      961 acaaaacgcc tgaagattat ttggcagtca gtgatgaaga acttatgaat gatattcgtt
     1021 ctatcggtct gtatcgtaac aaagctaaga atattaagaa actttgtcaa tctttgattg
     1081 atcaatttaa tggagaaata ccacaaacac ataaggaatt agaaagttta gcaggtgtag
     1141 gacgtaaaac tgctaatgta gtcatgagtg tagcatttga tgaaccttca ttagctgttg
     1201 atacgcatgt agaacgtgtt tctaaacgct taggtattaa tcgttggaaa gataatgtga
     1261 gacaggtaga agaccgttta tgttctgtca tacctagaga tagatggaat agtagccatc
     1321 atcaactcat tttctttgga agataccact gtttagcccg aaaacctaaa tgcgatattt
     1381 gtccactatt agaagattgt agagaaggac aaaaacgtta taaagctagt ttgaaagaag
     1441 cgtgataaca tgacaaatat tacaaaagag gtatttgata atttagaaca agagattgat
     1501 gtatttgcca aaaataagac attaggttct agtgaagcaa agccctactt ggatgaatac
     1561 catagtaaaa ttattgatta ttttaagcag gttaatgaca taactggcaa tattgatttt
     1621 gataaattaa atcaatatcc tgttgtgcca atgaatttta aagaaagata tgattatatg
     1681 attgaacgta aatatcattt tatgggctat cgacaaatga agacctttaa aactgaattg
     1741 attaaaatga atgctagtta tcaaacaagg ttaaagaata agcaggtata gacaagattg
     1801 atcatttcct aatgttgaaa atatataaaa ctaattcatg cacacatatt tgtatttatg
     1861 ttaggtgtcg tgtcattaca acgtcttaat ataaaaactg tccatctaca tcataatgga
     1921 tatcttgaga tatgctttaa gatacctatt tcgaatcgta ggtggatttc ttatatctat
     1981 gattgtgaaa atatgttgca aacatcttga tatttcactg attaacgtac acagggaatt
     2041 gataaacatt tcaatttaaa acagcgtcta agatgactat gagaaaaata atcataattt
     2101 taaaaatggg aacaaccact tttgcaatgt caataatttt aaatagctaa agatgtgaat
     2161 atgaaaaact atttagatgt ttcaaaatgt atgtgaatga ctgattttaa ggtttctact
     2221 aatatagcaa tagcgtatga atacttataa aaaaagcagg aagttttacc ttcccaccat
     2281 aaaagatgaa gaacctaaaa aatcctcagc tacgccataa atatggtata actgaggatt
     2341 ttatgttgtg tggattagtt gaatatacct gttaatccac cgctgtttct agattgttga
     2401 gatctagtat tgttatttga ttgtgcagta ccactgctgt ttgctgatgt gtcactacca
     2461 ccgtgtgtac tacgatttgt agtgttgtta tcttgactac cagaaacatt gattgatggg
     2521 atactaccac ttactgagct aggacgttta aagtcttcgc catctctaga tgaaattttt
     2581 gacatcacat tttcatataa gaactgtgga tattcttgtt ggctatgtcc cacaaatgag
     2641 ttttcaccat attgtttaac tttactgaag cccatccaca ctgacatagt gtattgaggt
     2701 gtaaagccgt taatccacac gtctttcgct gcattatcag gtaaattata ttgtgaataa
     2761 gtttcagcac cgtaagtacc agtacctgtc ttagcaccca tatttactcc agatacacca
     2821 tggccatatg cagaaccata tggtttaaat gtacctttta gcatctcagc taacatgtat
     2881 gcagtgtaat cactcatcgc tttatggcta gtatgatcgt attcgattgt ttcaccatca
     2941 cgagtaacta ctttttgaat tgaatgcgcg ttgttataag taccaccgtt agcgattgca
     3001 gcaaatgctg atgctaattg tgttggtgag aattctgaag cagaaccacc aagtacttca
     3061 gatggaccaa tatcgccttc gtagtttaag ccaagtttgg cagcgaattt cttaggtgca
     3121 tcattaccag cattttgctt aactgattgc caagctttta aagctggtat attgaaactt
     3181 tgtcgtaaag catcataaat agatacagta ccgtgactct tcgtatcata gtttctaaat
     3241 gtagaaccat caacttgata tgaagattca tcttgaatcg catggtttgt tgcccatttc
     3301 atattttcaa tggcaggtcc atacgctaag aaaggtttta aagatgaacc agtagggtga
     3361 ggatctgttg cttggtttct gttaacgacg tctttgaaat cacgtccacc agatatagca
     3421 actaaaccac cagttttact atcaagaatc gttgcaccaa cttgttggtc tttattcttg
     3481 tagaagctac cattatcaac atcattttgt aatgtttttt gaacatcttt atccatgttt
     3541 gtataaattt taataccact ttgtaataca ttacctaaat tttcatcttt gaatgcttta
     3601 ttattcatta attcagattt tacaaagtta acgtatgaat tatactcaga atcttgattt
     3661 gtatcaatgt tttgacgttc ttcagcagta cgatttacta agttcgcttt taaatcgatt
     3721 ttcttagcat cttcccactg tttatctgta atgcgtttat gataatgcat taagtataaa
     3781 acagtgtttt tacgatcttc agcagctttt ggatgatcat aaatattata gttgtttgga
     3841 acctgaggta aaccggctaa ataagcttct tccgctaagt ttaaatcttt taaatcttta
     3901 ttaaagtaat acttagcagc agctttaata cctgttacgc catcagaata gtaaatttta
     3961 tttagatata cttggaagat atcatcttta ctatactctt gttctaaacg atatgataag
     4021 tatgcttctt gagctttacg tccaatagat ttatgttgtg ataaaaatgc atctttaaca
     4081 acttgttgtg ttaatgttga ggcaccttca gaaccaaatc caccagtcaa gttcttacca
     4141 attgcaccga ataaacgttt ataatcaagt gcgccatgtt cgtagaaacg attgtcttca
     4201 gttgcaagta ctgcgtcttt cattgatttc ggcacgtctt ttaaatttac atgctcatgt
     4261 ctttggccat tatctaatgt tttaacaagt tctccgttct tgtcatatat ctttgcagga
     4321 atcggatctt gtaatttagc ttcggtaaaa gcaggtgctt tccaagcata ataagcaaac
     4381 aataagatac ctagtaaaag aacaacgaaa aatgcaataa tcatgaagcc aataatctta
     4441 ataatcgttc tcttcacatt tctattcttt tttgagttgg atttcccacc attattacca
     4501 tttttcttag gctgagaaga tcctttgttt tccgtcatac gcggtcctca ctttcatcta
     4561 atatcaactt atcaactgct tttagatagt ctaatcttgg ttgatactga taaggaatat
     4621 ggtaaccatt ttttcgtatt tcatcaactg ttatagactt tttaatatta tctttatatc
     4681 tcttccaaaa tacttcgaat tttgaatagg gtaaaagata aacttcatct agcgttttaa
     4741 aacgaatcat taaaaacaca ataccttttt gttgatatgc atttttcata tgttcgactt
     4801 gatggtcatg aatattattt aaaggaaagg acgtcttgtt tttagtttcc tttgcttcaa
     4861 aatcaatata ataaccttga taaacgccgt tgtaatcagt tgttgaaggt gtacgaaaat
     4921 aagcttcgtt aatcacagct ttactccgct taggatagtt gacattaact atttgtactg
     4981 gcgtaggctt tttgtgaata actgcaatgt cgcttttaag ataaaacgta tttgaatgtt
     5041 cgatatcttt ttcaagtgac ataccacgtc caccatactc aatattacta aaggcagcgg
     5101 tctttttccc tccgtctata gcactatttt tacgatatgg tttaccattt ggataattca
     5161 taaatttcac cacacaagtt tggtagtaaa cgttgtttat tatacacaac attatagtga
     5221 aacaccaaac ttaaattagt ataaatgcga aagacacaat acaccaaagc ttattttaac
     5281 gtgatttcag cattaacaca ataatattat aatacattta ccgttaatgt tggaatttta
     5341 cacacaattt taattaattg aaaagttttg cttataaaaa tgaatactca ttacaatatt
     5401 tttgaatttt gttataaaag tcaaatcatg at
//
LOCUS       DAMZLE010000143         5418 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMZLE010000143 DAMZLE010000000
VERSION     DAMZLE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.145"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            220..330
                     /locus_tag="POB92_002318"
     CDS             220..330
                     /locus_tag="POB92_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6579785.1"
                     /translation="MVRLYKNGKPRNEIIREYDLTPSALGKWIKQHQNTG"
     gene            453..1127
                     /locus_tag="POB92_002319"
     CDS             453..1127
                     /locus_tag="POB92_002319"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6579786.1"
                     /translation="MEVIQKNAHQYSVPAMCKVLKIPRSTYYDSIKRKDNKITKDDSN
                     VERAVINIFNSNRKVFGARRKIARIMKKHNLVSVYTKAKYKNYPKETNEKRIKNHLNR
                     AFNREQPMETLVSDLTYVKVAGTWHYICLFIDLFNREIVGYSAGKNKDANLVSKAISR
                     INHNLEQINLFHTDRGKDFDNHLIDEVLESFKIKCSLSTKSCPYDNAVAEATMKAMRS
                     EFVKQM"
     gene            1131..1259
                     /locus_tag="POB92_002320"
                     /pseudo
     CDS             1131..1259
                     /locus_tag="POB92_002320"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1599..2855)
                     /locus_tag="POB92_002321"
     CDS             complement(1599..2855)
                     /locus_tag="POB92_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HDI6579787.1"
                     /translation="MIDNFKQVLKLIKEKRTNNVVKKSDWDKGDQYKTLVHDKLPKQL
                     KAHIKEDKYSVVGKVATGNYSKVPWISIYDENITKESKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISNMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRIKYYDLNEGFTEEEMLYDLKKFLELFNELASKVTKTFYDGLVNRIDE
                     IQEDSEIEEIRIAQIDKTLKQVEAPKGVIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALKYFNDLIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDEAIEK
                     FIEIKTSISSNIEEPFFMSLNEMFALKEYKKKYLILRIFNVSGEEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(3186..4115)
                     /locus_tag="POB92_002322"
     CDS             complement(3186..4115)
                     /locus_tag="POB92_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDI6579788.1"
                     /translation="MKKSVRLYHMIEYCNEKRNFKLNDLMSEFNISRSTALRDIKEIE
                     ALGVPLYSSTGKNGGYMTIGNRNQTKIAITDEELKALVFTLSSISNVSKLPFQTEYQE
                     ILKKLYNNSNKKELINQYNEIFQYFNEDTYQFKSYKIFNEIIRLTIGSKSFKICYSQN
                     YLKEQYKGIGILYKNQQWYFVVVNIASKLVKLLNISKIKEVYEMGETKECNEITMQNF
                     QQFMVENENTIDIQIRSNIIGLNILKGYLWSDYMIENINEETYLFKSKVNMQDINFIA
                     KLIVTCGANVKVEFPISLKKAIKTELTKIINLY"
     gene            4195..4623
                     /locus_tag="POB92_002323"
     CDS             4195..4623
                     /locus_tag="POB92_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6579789.1"
                     /translation="MNFQVSPYFLIIENADGLKRLYEELFGAKTLYIQRIKDRPTDKQ
                     LNIDKKDLNKIDQCVLQFGDIKIMIADDTEGLPITQGNNISLCITFDHVEETKRVYDQ
                     LIEKGSEVLKAFSPEFYTEGYGYVRDPYGISLHLFTKRKG"
     gene            complement(5045..5287)
                     /locus_tag="POB92_002324"
     CDS             complement(5045..5287)
                     /locus_tag="POB92_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579790.1"
                     /translation="MLNIEASIKDIQDIINYFIKCDVNNKYYESKQRSSLEKLALKLD
                     KVSNQLNNENIEGMKKIIEKVAEANRVIAKSSLRKN"
BASE COUNT         1861 a          790 c          698 g         2069 t
ORIGIN      
        1 attcatagat atcagttgct atagtagtta gaatggaaat gatagtttaa taatgtacat
       61 tttttaaaaa cctagacata attttattga ggtgaacaat gaatcgtcta cacttaattg
      121 gacacattct atgagagtag attttgttaa tttaagaaag taggcgattt tattatgact
      181 agagaaagaa gatcatttag ttcaaatttt aagttataaa tggttagatt atataaaaat
      241 ggtaagccta ggaatgaaat tatacgcgag tatgatttaa caccttccgc gttagggaaa
      301 tggataaagc aacatcaaaa cacaggttga ttcaatcatc aagatagctt aacagatgaa
      361 gaaaaagagc tgattaaatt aggcaatgaa gttcaacata taaaaatgga gaacgatatt
      421 ttaaagcaag cagcgctgat cataggacga aaatagaagt cattcaaaag aatgcacatc
      481 aatattcagt tccggcaatg tgtaaagtcc taaaaatacc aagaagtacc tattatgatt
      541 ctataaaaag aaaagataat aaaatcacta aagatgattc aaacgtagaa cgtgctgtca
      601 taaatatttt taattctaat agaaaagtct ttggtgcaag acgaaagata gctcgtatca
      661 tgaaaaaaca taatctagtt tctgtttata cgaaagctaa atacaaaaat tatccaaaag
      721 aaactaatga aaaacgaatt aaaaatcatt tgaatcgcgc ttttaataga gaacaaccaa
      781 tggagacatt agtaagtgat ttgacatatg taaaagtcgc aggaacatgg cattacatat
      841 gtttatttat agatctattt aatagagaaa ttgttggtta cagtgcaggt aaaaataagg
      901 acgcaaattt agtatcaaaa gcaatcagca gaataaatca taatcttgaa caaatcaacc
      961 tgttccatac cgatagaggc aaagactttg ataatcattt gatagatgaa gtcctagaat
     1021 cctttaaaat caaatgttca ttaagtacca aaagttgtcc ttatgataac gcagttgcgg
     1081 aagcaacgat gaaagcaatg agatctgaat ttgtaaaaca aatgtaattt gaaaacttag
     1141 aacagttaga aacagaatta tttgattatg taaattggta taacaatttt agactacatt
     1201 cttcattaca gtatttgacg ccagtggcat ttaaaaatct acatatgaaa aatgtctaaa
     1261 aaaatgttga cataccaggt tatagaatac caattggaag tatttatgag tttcatcgaa
     1321 gatgttaaac tgtccagctt aatttgacaa tctaaatgta aaatcattaa taattaaaca
     1381 aaacttaaaa tatttaactt tttgtgatta ttccacatta tcttatctgc tctttatcac
     1441 cataaaaata gaaaaaacaa gattcctaaa gaatatagga atcttgtttc agactgtgga
     1501 caaactgatt ttttatcagt tagcttattt agaaagtttt atttaaatta cagtttctat
     1561 attttattag atcactattt tagctcttgt tcaagtaatc atttttcgcc aaaaacttta
     1621 tattgaatag cttctacatt aaatacttta tcaatgagat catctacatc tttaaattca
     1681 gaataatttg catacggatc tataaaataa aattgtggtt cttcaccaga aacattaaat
     1741 attcttaata ttaaatattt cttcttatac tctttcaaag caaacatttc atttagcgac
     1801 ataaaaaatg gttcctcaat attagaagat atagatgttt taatttcaat aaatttttct
     1861 atagcttcat ctgtatttgt tggatcaaaa gctactaaat catagccatg accgtgttga
     1921 gagcctggat tatcatttaa aatattccta aactgttctt tcttatcttc gtctatttta
     1981 ttatcaatta gatcattaaa gtattttagc gctaattttt ctccaacttt accggttaat
     2041 ttattctctt tatttgattt ttcaatttct gaatcatttt tagtagtctt tgatacacct
     2101 tttttatatt ttggaattac tcctttaggt gcttccactt gcttgagtgt cttatctatt
     2161 tgtgctattc taatttcttc aatttcacta tcttcctgta tttcgtctat tctattgact
     2221 aagccatcat agaatgtttt tgtaactttt gaagctaatt cattaaatag ttctaaaaat
     2281 ttctttaaat catatagcat ttcttcttct gtgaaccctt cattcaaatc ataatatttg
     2341 attcttattg atccatgaga atatcctgat gggtaatcat tttttaaatc ataagatgaa
     2401 tctttatttt ctgcgtaata aaatcttcca gtattaaatt catttgatgt aatatattta
     2461 ttgagttcgg aagataaagt taatgctctt tgttttgcag catttttatc ccgcggaaac
     2521 atattactta tctttgacca tccttgattc aaagataagt atatgccttc tccttctgga
     2581 tgaaaaagat ataccaaata atatccatcc tttgattctt ttgttatatt ctcatcatat
     2641 attgaaatcc aaggaacttt actatagttc ccagtagcaa ccttccctac aactgaatat
     2701 ttatcttctt ttatatgcgc ttttaactgc ttgggtaact tatcatggac taaagtttta
     2761 tattgatcac ctttatccca atcagatttt ttaactacat tattggtacg tttctcttta
     2821 attaatttaa ggacctgttt aaagttgtct atcattggaa attcgctcct attataaaat
     2881 atattatgtc tcattttctt caatatgtac ttatttatat tttaccgtaa ttttactata
     2941 tttatttaca gaaataattt tctcaaagct aaaactttgc ttcactataa atattcacta
     3001 taaagaatat ttcgatatta tttacttgaa agactgcgga ggctaactat gtcaaaaatc
     3061 atgaacctca ttacttatga tacgcctctg cgtctcggtt agtgatgcgg tttttaaact
     3121 acggatcaat ttaatatgtt taagattatc ttttctttgt tattgcaatc ataattaaat
     3181 taccattagt ataagtttat tattttggtt agttcagttt taatagcttt tttcaaacta
     3241 ataggaaatt ctactttgac attggcacca catgtgacta ttaatttagc tataaaattt
     3301 atatcttgca tgttcacttt tgatttaaac aaatatgtct cttcgttaat attttcaatc
     3361 atatagtcac tccacaagta tcctttcaag atatttaatc caataatatt acttctaatt
     3421 tgtatatcaa tcgtattttc gttttcaacc ataaactgtt gaaaattctg catagttatt
     3481 tcattacatt ctttagtttc tcccatttca tatacttctt ttattttaga aatgtttaac
     3541 aactttacta attttgatgc tatattaaca acaacgaagt accattgttg atttttatat
     3601 aagataccaa tacctttata ttgttctttc aaatagtttt gtgaataaca gattttaaat
     3661 gacttactac caattgttaa tctaatgatt tcattaaata tcttataact tttgaactga
     3721 tatgtatctt cattaaaata ttgaaatatc tcattatact ggtttatcaa ttctttttta
     3781 ttcgaattat tatataactt ttttaatatt tcttgatatt ctgtttgaaa aggtaactta
     3841 ctaacattgg agatacttga aagtgtaaat actaaagctt tcaattcttc gtctgtgatt
     3901 gctattttcg tttggtttcg attacctatg gtcatataac cgccattttt ccctgtacta
     3961 ctatataaag gcactcctaa tgcttcaatt tcctttatat cccttaaagc agtactacga
     4021 gaaatattaa attctgacat taaatcattt aacttgaaat tccttttttc attacagtat
     4081 tcaatcatat gatataatct aacagatttt ttcattatta ttcccctttt agtatcaata
     4141 ggtgacacta atacagtata tacttattta taacaaattt aaggaggaaa aactatgaat
     4201 tttcaagtaa gtccctattt tttaattata gaaaatgcag atggtttgaa aaggttgtat
     4261 gaagagctgt ttggcgctaa aacactgtat atccaacgta tcaaggatag accgacagat
     4321 aagcaactta atatcgataa aaaagattta aataaaattg atcaatgtgt acttcaattt
     4381 ggagatataa aaattatgat tgcggatgat accgaaggtt tacctataac ccaaggtaat
     4441 aatatatcgc tttgtattac ttttgaccat gtagaagaaa ctaagcgtgt gtatgaccag
     4501 ttaatagaaa aaggcagtga agtattaaaa gcattcagcc cagagttcta tactgaaggt
     4561 tatggttatg taagagatcc ttatggtata tctttacatt tatttacgaa aaggaaaggt
     4621 taaaccaagt ttctagtgtt ctattacaga tattggtaca actataagga catttgggaa
     4681 caaattatcg catgtttttt caatataaaa agccatgaca ttatgcatct tggctttcgt
     4741 ttaatcttat acctatttaa tagtttaata cctactgtac agaatgaaca gtacagttat
     4801 gacgtgcttt tagttgtgat tctattatat gtatagtatt tgaacttaag gcaaaatatg
     4861 caataccccg tataagcgat gtttttcatt atcaatgatt ttggaacggg aaagggtgag
     4921 gattcagaat ggtttcccgt ttcttcattt agatataagt ttttcaagtt ttaactcaat
     4981 atatatttaa aatttctttt gattagtaga tttattatca acttcaatca tcttactaaa
     5041 acactcaatt ttttcttaaa gaagatttag cgataactct atttgcttct gctacttttt
     5101 caattatctt tttcatacct tcaatgtttt cattgtttaa ctgattactt actttatcca
     5161 attttaaagc taacttttcg agactgcttc tttgcttact ttcataatac ttattattaa
     5221 cgtcacattt aataaaataa tttataatat cttgaatatc cttaatacta gcttctatat
     5281 ttaatattga cttacctcca cttattcaaa attattttgt atctcatatt ttttaatttt
     5341 attagtgagt aatgaaaaat caaattttct aatttcctga tttacattaa tttcagatat
     5401 cttctcaact aatttctt
//
LOCUS       DAMZLE010000144         5384 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMZLE010000144 DAMZLE010000000
VERSION     DAMZLE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.146"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..318
                     /locus_tag="POB92_002325"
     CDS             <1..318
                     /locus_tag="POB92_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6579791.1"
                     /translation="DSNYRLSNVDTMKVTLYSNGSNYDKEALLINKDEFCPLRKITLD
                     NKLDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKRDIVQDISKIFEVNYYTYNHI
                     MHH"
     gene            752..1354
                     /locus_tag="POB92_002326"
     CDS             752..1354
                     /locus_tag="POB92_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579792.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSALLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNIMER"
     gene            1621..4773
                     /locus_tag="POB92_002327"
     CDS             1621..4773
                     /locus_tag="POB92_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDI6579793.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            4830..5312
                     /locus_tag="POB92_002328"
     CDS             4830..5312
                     /locus_tag="POB92_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579794.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         2062 a          660 c          974 g         1688 t
ORIGIN      
        1 gactcaaatt atagacttag taatgttgat actatgaaag taacgcttta cagtaatgga
       61 agtaattatg ataaagaggc tttactaata aataaagatg agttttgtcc tttaaggaaa
      121 ataactttag ataataagct tgattcacaa cgggtaatgg aaattgattc attggcagca
      181 ataataaact tagttaaaca aggaaaagga aaggctttat tacctatgac ttttgagaat
      241 aaaagagata tagtacagga catttctaag atatttgaag ttaactacta tacttataat
      301 catattatgc atcattaatt gaaagttgat tgagtttaaa ttacaggaac gattttaaaa
      361 gtctagtagt tagaatgagc gatgtgacag aaaagaaatt atgcctttta ttttgtaaat
      421 tcgttgtttt aaagtaaagg tccttgtagt ataggaaaaa gtggatgact tctccgcctt
      481 taccaccctt tcaagcataa tgataactgc taaatattga ttatagcacc attgcaaaac
      541 taatcattaa ttgtcatatt caactatttt gaaaagtatc aatttcacat tatcaaatta
      601 ttgagtatct caatttattg ttatacacaa acagtatttt tgttatcata gagttaataa
      661 atataataaa ttaaacaagt aactttacaa aaattagaat gattcaattc aatcataatt
      721 gtttgaaaat agaggtgagc aggtgaacga tatgttaatt agtcttgtaa tcccagtttt
      781 tgctttgtta gttattggtg gtattatttg gatgattata gaaggtatag tacatatttc
      841 aaaaaagaat aaagcaattg ataacttttt taaccaagtt aataaagtaa gtgagacata
      901 taaattcgct actacttttt tatttctaat cttggctacg gcgggtattt ctcaatttta
      961 tctatattat atagtatcag cgttactttt ttggcttgtc atacttacct ttggtattgc
     1021 aggtatcatt tttttaatgc catatggatt atgttttcta ccgttttata agcaaaaaaa
     1081 gaaaaaacag acatttaaaa aatacatggt ttacactacg attggtttgt caatttgtct
     1141 aggcttatct ctagttttgg ttcacactac gaaaatttat atggacgaag gtggcgtaag
     1201 atactattac ggtagttttg taatgaaaca agcgggcggt tatgcttatt tagctttagc
     1261 agtactttca acgttgttaa ttgttgcgaa aaaagctaca aataaaaata aagaaatcga
     1321 aaccgtcgac aatacaaata taatggaaag ataattaagg gagtgctcat tcaggagtgc
     1381 tcttttttga tgttcaaatt tagttggaaa tgaaggcata gaagaaattt ttatagtcat
     1441 ttattggata atgaattatg gtcatatcga ttaattttat tagtgaggat tttacaaaca
     1501 tttgttgaat aaatatggta atgataatat atgaatattt ttgaaaaaac actaatatga
     1561 ttaaattatt tgttaaaatg gcattaagta aaaaacaaac agggaacaag gtgggatttc
     1621 atgagtcaat tgctaaatga tacgttatcg gcttggttgt taattgaatc tttaagtcca
     1681 ggagaagtaa attttacagc ggaagatata ctctcagctg aacattttaa aaatggtgca
     1741 aagcaagcac aacttcaaag ttttgatgaa tattttgaaa tatggaatgc tgaacgcttt
     1801 attatatcag aagaaaaatc agagactggg gaacttatat ttaaatttta tagacattgc
     1861 ttccgctata atgaaattaa tttgaaaatt caggatattt ttgatgatta ttctgagatt
     1921 cataatccaa atgggacaca ctgttatggt tacacattta acacagataa acacggcaaa
     1981 gtgatagttg attctataca tattccgatg attatgagtg cattaaaaga aattgaaaag
     2041 aacaaaaatg ccaatataga agaaaaattt aatgattctg ttgaaaaatt tgttcaaaaa
     2101 gtaaaagaaa ttttagcaga tgaaccaatt aatgaattta aattgaagaa gatggacaaa
     2161 gcttatgatg agtacttttc tgtattaaat tcaaagaaag atggattatt tggacattat
     2221 gtagcaatag aatatgtgaa agatagtgat ttaccacagc cggaatttaa cagcttcttc
     2281 ataagtgata ttgagaaagc aagaaaatct cccaaccaaa ctttaattga ttacattgaa
     2341 ggtgtagaag aaagtcagcg catagaagta gatgaaaata aagaaatgtt tgacaaattt
     2401 ttacatcctt cacgtttgcc tgatggacga tggccatcac agactgagtt tagattgtct
     2461 ttaatgcaac aacttgctgt aaaccaaatt acgagtggta atgaaagaat aagttcagtt
     2521 aatgggccac caggtacagg taagactact ttattaaaag atatatttgc tcatctagta
     2581 gttgaaagag gtaaagagtt agctaaacta aataatccta aagatgcatt tgtcaaaaca
     2641 aaaattcatg aaacggatga taaatacgta tacttactaa aggaatctat tgccaaatat
     2701 aagatggtag tcgcatctag taataatgga gctgttgaaa atatatctaa agatttaccg
     2761 aaaattgaag aaattataag aaatcccgaa aaatgtaaat tccctaaata tgaacagaat
     2821 tatgcaaatt tagcacatga attaaaagat tttgctgaaa tagctgaaga tttgattggt
     2881 gaaagtgcct ggggcttatt ttctggagtt tttggtaaaa gtactaatat taaccaagta
     2941 ttgagtcata tgttaaaaca agatgcgaat gatattggct ttgctaaatt actacaaaat
     3001 gagaataatc gtatgagtta taacgagtta atgagtgaat ggcaatcaca tcaacgtgca
     3061 tttttagaag agttgaggca tgttgaaatg ttaaaagaag aatctattag agcatatgat
     3121 gtttataaaa attgtgagtc tttctctaag attgaacagg ttattaatag tgaaaaaaca
     3181 agtattgaag aacaggtata tcatttagat aatgaaacgt tacgagacaa taaagaaata
     3241 gaagatttgg ataatcgaat taattatatt gttaagcaaa tagaaacttt aaatgagtta
     3301 attaaatcca tcaaagaaag caacaaaggt tttattaaca aactgaaagc gatgtttaat
     3361 tcagaagaag atgaaagcta taaagatcat aataaagaga agcaacaatt attaacacaa
     3421 cagttagagt tagagaaatg taaaaaaaac aaacatgaag accttgttag caaactaaaa
     3481 gaaaaagaga aattaattaa acaattaact aaagtacagt tgcaattaga cgagttaaat
     3541 tcacagttac aagagttaga agcatatcgt attgagtcaa aaattacaat tccagaaaaa
     3601 gatttttgga gtgacaacaa ttatgatgag cgccaagtta ctaatctgtg gacgagtgac
     3661 gaacttcaat acagacgtgc catgctcttt ttaagagcaa tgatattgca taaattatta
     3721 ttgattgcta ataatacaac tatttattat gcgattaatg attttaaaga tagaaggaaa
     3781 ttaattgatg caaatccaga taaagtacac aacgcatgga atgtgatgca tttaatattt
     3841 ccagtagtta gtacgacgtt tgcaagcttt aaatctatgt atgggggcat accaaaagat
     3901 ttcatagact acttatttat tgatgaagca ggacaagcaa tacctcaagc agctgtggga
     3961 gcattatatc gttcaaaaaa agttgtagct gtaggtgatc cgattcaaat agaaccggtt
     4021 gtgactttag aaagtcattt aattgataac attcgtaaaa attatcatgt tccggaatat
     4081 ctagtttcta aagaagcttc tgtgcagtct gttgcagaca acgccaatca atatggtttt
     4141 tggaaatctg atgctactga tagtaatcaa aaaacctgga taggcatacc tttatgggtg
     4201 cacagacgat gtttaaaacc tatgttcacg atagctaacc aaatcgctta taataataaa
     4261 atggtgttgc caagtaatat tacaaaagta ggtaaaacag gttggtatga cgttaaagga
     4321 aacgcagttc aaaaacaatt tgtgaaagag catggtgaaa aagtagtggg attattagct
     4381 gatgattgga ttgaagcaat taaggaaggt aaaaatgaac cgagctcatt tgtaatatcg
     4441 cctttttcag cagtacagca acagattaaa cgtatgttaa agcaacaact accgactaga
     4501 attgatattg aacgtacaaa aattaatcaa tgggtcgata aatccattgg tactgttcat
     4561 acttttcaag gtaaagaggc tcagaaggtg tattttgtaa taggtactga taatacccaa
     4621 gatggtgctg tgaactggtc atgcgaaaaa ccaaacttgt taaacgttgc agtgacaaga
     4681 gctaagaaag agttttatgt aattggcgac atgcaaagaa tacagatgaa accattttat
     4741 gagacgattt ttaaagaaag aaatgtaaaa taacatacaa aaaagtatat agaggaagtt
     4801 atacatttta aaaggagcaa aattgaataa tggagaaatt taacaactgg atattaaatg
     4861 caataagtgg atctcaaaca gacaagaatg aaacaactga agaattaaaa ggggcaaaat
     4921 ttatcatttt atatgcatat tcaatgctcg ttttgcttgc gttagtaatt tctaacatat
     4981 tcattcacat tttggagcct aaactatcaa tcaccactca aatcatcatc gttttgattt
     5041 taattgaagc actaattgga ctgcgtttct tgaaagcgta cgatgttaag cgtggcaaag
     5101 ataaagaaaa taagaaaaat agtaaggatt tcgttaaact aaaatcaatt ttagtagcaa
     5161 ttttatttac atcattggcg ctgacagcag gtactgtagc tgatatatac ggtttcactg
     5221 acttaggaaa tactagaagt gatttaatcg tttggagcat aggtggtatt atatttggcc
     5281 tcgtatgtta cacaatggaa gataaaagat aacgataagg agctggcgat tataaagcta
     5341 gctccttttt taacttatat atgtaaagaa ctatcctaag ggtt
//
LOCUS       DAMZLE010000145         5322 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMZLE010000145 DAMZLE010000000
VERSION     DAMZLE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.148"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            43..1161
                     /gene="ald"
                     /locus_tag="POB92_002329"
     CDS             43..1161
                     /gene="ald"
                     /locus_tag="POB92_002329"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6579795.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLQDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1256..2296
                     /gene="tdcB"
                     /locus_tag="POB92_002330"
     CDS             1256..2296
                     /gene="tdcB"
                     /locus_tag="POB92_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDI6579796.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2327..3649
                     /locus_tag="POB92_002331"
     CDS             2327..3649
                     /locus_tag="POB92_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6579797.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3806..5197
                     /gene="norB"
                     /locus_tag="POB92_002332"
     CDS             3806..5197
                     /gene="norB"
                     /locus_tag="POB92_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDI6579798.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVSSLLFITLLAITIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1837 a          760 c         1022 g         1703 t
ORIGIN      
        1 ataattgtga aattattcac aaatgaaaga ggagtgttgc aaatgttagt tgcagtagtc
       61 aaagaattaa aacaaggtga aggacgtgta gcttgcacac ccgaaaatgt gcgtaagtta
      121 acggatgcag gacataaagt aattgttgaa aaaaatgctg gcattggttc aggattttct
      181 aacgatatgt atgaaaaaga aggcgctaag atcgtaactc atgaacaagc atgggaagct
      241 gatcttgtta tcaaagtaaa agaacctcat gaaagcgaat atcaatattt caaaaagaat
      301 caaattatct ggggattttt acatttagca tcttcaaaag aaatagtaga aaaaatgcaa
      361 gaagttggtg taactgcgat tagtggtgaa accattataa aaaatggaaa agcagaatta
      421 ttagcgccaa tgagtgctat agcaggtcaa cgctcagcaa ttatgggagc ttactactct
      481 gaagcacaac atggtggtca aggtacttta gtgactggtg tacatgaaaa tgtggatata
      541 cctggtagta catatgtgat tttcggtggt ggagtagcag caacaaatgc agcaaatgtt
      601 gccttgggac taaatgctaa agtaatcatt atcgagttaa acgatgatcg cattaaatat
      661 ttacaagata tgtatgcaga aaaagatgtc acagtagtca aatcaacacc agaaaattta
      721 gcagaacaaa taaaaaaagc agatgtattt atttctacaa ttttaattcc tggtgcgaaa
      781 ccgccaaaat tggttactcg tgagatggtt aaatcaatga aaaaaggttc agtattaatc
      841 gatatagcta ttgaccaagg tggaactatt gaaacaatta gaccaactac aatttctgat
      901 ccagtgtatg aagaagaagg tgtgattcat tatggtgtac caaaccaacc aggagcagtc
      961 ccaagaactt caacaatggc attagcacaa ggaaatattg attatatatt agaaatttgt
     1021 gacaaaggct tagaacaagc aattaaagat aatgaagcct taagtactgg tgtaaacatt
     1081 taccaaggac aagtgacaaa tcaaggatta gcttcatcac atgacctaga ttataaagaa
     1141 atattaaatg ttatcgaata gataataatt taaatgaaat tgagtgaaat gaatatttta
     1201 aatatagcat tatagtttgg actaaaaatt tacaaaacgg aaggatgtaa ttattatgac
     1261 aaccaacaca gttacattac aaacagcaca tattgtaagt ttaggagata tcgaagaagc
     1321 taaagcaagc attaaaccat ttattcgtcg aacacctcta attaaatcaa tgtatttaag
     1381 ccaaagtata actaaaggga atgtatttct aaaattagaa aatatgcaat tcacaggatc
     1441 ttttaaattt agaggcgcta gcaataaaat taatcactta acagatgaac aaaaagaaaa
     1501 aggcattatc gcagcatctg ctgggaacca tgcacaaggt gttgctttaa cagctaaatt
     1561 attaggcatt gatgcaacta tcgtaatgcc tgaaacagca ccacaagcga aacaacaagc
     1621 aacaaaaggc tatggggcaa aggttatttt aaaaggtaaa aactttaacg aaactagact
     1681 ttatatggaa gaattagcga aagaaaatgg catgacaatc gttcatccat atgacgataa
     1741 gtttgtaatg gcaggccaag gaacaattgg tttagaaatt ttagatgata tttggaatgt
     1801 gaatacagtc atcgtaccag ttggcggtgg aggattaatt gcaggtattg ccaccgcatt
     1861 aaaatcattt aacccttcaa ttcatattat cggtgttcaa tctgagaatg ttcatggtat
     1921 ggctgagtct ttctataaga gagatttaac tgaacatcga gtggatagca cgatagcaga
     1981 tggttgtgat gtaaaagttc ctggtgaaca aacatatgaa gtagttaaac atttagtaga
     2041 tgaatttatt cttgttactg aagaagaaat tgaacatgct atgaaagatt taatgcagcg
     2101 tgccaaaatt attactgaag gtgcaggcgc attaccaaca gctgcaattt taagtggaaa
     2161 aataaacaat aaatggcttg aagataaaaa tgttgttgct ttagtttcag gcgggaatgt
     2221 tgacttaact agagtttcag gtgtcattga acatggactg aatattgcag atacaagcaa
     2281 gggtgtggta ggttaaaaca tttaatctta aaaatgaggt gtaattatgt caaatggtaa
     2341 agaattacaa aaaaatatag gtttcttctc agcgtttgct attgttatgg ggacagttat
     2401 tggttcagga gtattcttta aaatatcaaa cgtaacagaa gtaacaggaa cagcaggaat
     2461 ggccttgttt gtatggttcc taggcggcat cattaccatt tgtgcggggt taacagcagc
     2521 agaacttgct gctgcaatcc ctgaaacagg tggcttaacg aagtatatag aatatacata
     2581 cggtgatttc tggggcttcc tatcaggttg ggcgcaatca tttatttatt ttccagctaa
     2641 cgtagcagca ttgtctatcg tatttgcgac acagctaatt aatttattcc atttatctat
     2701 aggttcgtta ataccaatag caatcgcatc tgcgttatct attgtgttga taaatttcct
     2761 aggttcaaaa gcaggcggaa ttttacaatc agttacttta gtaattaaac tgattccaat
     2821 catcgttatt gtaatttttg gtatttttca atctggagat atcacttttt cattaattcc
     2881 aactacaggt aattcaggaa atggtttctt tacagcaatt ggtagtggtt tattagcaac
     2941 tatgtttgca tatgatggtt ggattcatgt aggaaatgtt gcgggggaac ttaaaaatcc
     3001 taaacgcgat ttacctttag cgatttcagt tggtatcggt tgtattatgg ctgtgtattt
     3061 attaattaac gctacattct tattaacgtt gccaatagaa ctacttgctg gtaatttaaa
     3121 tgcggcttca gatacatcaa aaatattatt tggtgaaaat ggcggtaaga ttattacaat
     3181 cggtatatta atttctgttt atggtacgat caatggctat actatgactg gtatgcgcgt
     3241 accatatgca atggctgaaa gaaaattatt gccatttagc cacttattcg caaaattaac
     3301 aaaatctggc gcaccatggt ttggcgcaat tatacaactt ataatcgcta tcatcatgat
     3361 gtcaatggga gcatttgata caattacaaa catgctaatc tttgttattt ggttgttcta
     3421 ttgtatgtca tttgttgcgg taataatttt aaggaaacgc gaaccaaata tggagcgacc
     3481 atataaagta ccgttatatc caatcatacc tttaattgca attttggcag ggtcatttgt
     3541 attaattaat acactgttta cacaatttat actagcaatc attggaattc taataacagc
     3601 acttggtata ccagtttatt attataagaa gaaacaaaaa gcagcataag gtaagataac
     3661 tagcattaag aataaatgga tggactacta ataaatttaa agttttacac attaaaatca
     3721 aaaaccattc aattattcta tggaacagac aaatttctgt tatggaattt gtctgttttt
     3781 caaaagtata gggaggcaaa tagagatgga aaagccgtca agagaggcat ttgaaggcaa
     3841 taataagttg ttaataggaa ttgttttaag tgtaataacg ttttggctat ttgcacaatc
     3901 attggttaat gttgtaccaa tacttgaaga tagtttcaat acagatattg gaacggttaa
     3961 tatcgccgtt agtataactg ctttattttc aggaatgttt gtagtaggag caggtggtct
     4021 tgctgataaa tatggcagaa ttaaactcac gaacattggt attatcttaa atatattggg
     4081 ttcattatta atcattattt caaatattcc tttattactt attataggaa gattaattca
     4141 aggactttca gcagcatgta ttatgcctgc aactttgtct attattaagt catattacat
     4201 tgggaaagat agacaacgcg ctttaagtta ttggtcaatt ggctcatggg gcggctctgg
     4261 tgtttgttca ttttttggag gtgcagttgc aacgctttta ggttggcgtt ggattttcat
     4321 cctatcaatt ataatttcat taattgcgct ttttcttatt aaaggcacac ctgaaactaa
     4381 atctaaatcg atttctctaa ataaatttga cattaaaggt ctggttcttt tagtcattat
     4441 gcttctcagt ttaaatattt taattactaa aggatcagaa ttaggtgtta gctcacttct
     4501 ttttatcact ttattagcaa ttacaattgg atcttttagt ttatttatag ttcttgaaaa
     4561 gcgtgctaca aatcctttaa tcgattttaa attatttaaa aataaagctt acacaggtgc
     4621 aacagcttca aactttttgt taaatggtgt tgcaggaaca ttaatagtag ccaacacatt
     4681 tgttcaaaga ggtttaggat attcttcatt gcaagcagga agtttatcaa tcacttattt
     4741 agtaatggta ctaattatga ttcgtgttgg tgaaaagtta cttcaaacac tcggatgcaa
     4801 gaaaccaatg ttaattggaa caggagttct tattgtcgga gaatgtctca tttcattaac
     4861 tttcttgcca gaaatattgt atgtcatttg ttgtattata ggttatttat tctttggttt
     4921 aggactaggg atatatgcta caccatcaac agatacagca attgcaaatg caccgttaga
     4981 aaaagtaggc gttgctgcag gtatctataa aatggcttct gcattaggtg gagcatttgg
     5041 cgtcgcattg agtggtgcag tatatgcaat cgtatcaaat atgacaaaca tttatacagg
     5101 tgcaatgatt gcattatggt taaatgcagg tatgggaata ttatcattcg ttatcatttt
     5161 gttacttgtg cctaaacaaa acgacactca attatgataa ttgagaatta aattgaaatc
     5221 atacaagtcg ctacaatatt aaacaaaaat ataaaccgat tcttatgtgt cattatttta
     5281 aatgaacata gggattggtt ttttattact cttttacgct ac
//
LOCUS       DAMZLE010000146         5309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMZLE010000146 DAMZLE010000000
VERSION     DAMZLE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.149"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            44..2662
                     /gene="mutS"
                     /locus_tag="POB92_002333"
     CDS             44..2662
                     /gene="mutS"
                     /locus_tag="POB92_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI6579799.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            2675..4684
                     /gene="mutL"
                     /locus_tag="POB92_002334"
     CDS             2675..4684
                     /gene="mutL"
                     /locus_tag="POB92_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI6579800.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMVENQNDEIVIR
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4690..5232
                     /locus_tag="POB92_002335"
     CDS             4690..5232
                     /locus_tag="POB92_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDI6579801.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT         2097 a          695 c          978 g         1539 t
ORIGIN      
        1 aaagacaaat tttaaaaaga gacaagggtt gtgaagaaat aatatgtcta atgttacacc
       61 aatgatgcag caatatttaa aaataaaatc agaataccaa gattgcttat tattttttag
      121 actaggtgat ttctatgaaa tgttttatga agatgccaag gaggcatcac gtgtacttga
      181 aattacttta actaaaagag atgctaaaaa agaaaatcca attccgatgt gtggtgttcc
      241 gtatcattct gcagatagtt atatagatac acttgttaat aatggatata aagtagctat
      301 ttgtgaacag atggaagatc cgaaacaaac gaaaggtatg gttagacgtg aggtagtaag
      361 aattgtgact ccaggaactg tgatggagca aggtggtgta gatgataaac aaaataacta
      421 tattttaagt tttgttatga atcaacctga aattgcgctt agttactgtg atgtttctac
      481 tggcgaatta aaggttacac attttaatga tgaagcgact ttattaaatg aaattacgac
      541 gataaaccct aacgaagttg ttatcaatga caatatttcc gataatttaa aaagacaaat
      601 taatatggtg acagaaacaa taacagtcag ggaaacgtta tcatcagaaa tctatagtgt
      661 gaatcaaact gaacataaat taatgtatca agcgacacaa ttattgctag attatattca
      721 tcatacacaa aaacgtgatt tatcgcatat cgaggatgtt gttcaatatg cagctataga
      781 ttatatgaaa atggattttt atgctaagag aaaccttgag ttaacggaaa gcattcgatt
      841 aaaatcaaaa aaaggaacgc tactttggct aatggacgaa acgaaaacac caatgggagc
      901 acgccgctta aaacaatgga tagatagacc actaataagt aaagaacaaa ttgaagcacg
      961 attagatatc gttgatgaat ttagtgctca tttcatagaa agagacacct taagaacata
     1021 tcttaatcaa gtgtatgata ttgaacgtct tgttgggcgt gttagttacg gaaatgttaa
     1081 tgcgagagat ttaattcaac ttaaacattc catttctgaa ataccgaata ttaaagcatt
     1141 actaaattct atgaatcaga atacgcttgt acaagttaat caactagaac cccttgatga
     1201 tttacttgat atattagaac agagtttagt agaagaacca ccaatttcag ttaaagatgg
     1261 cggactattc aaagttggtt ttaatacgca attagatgaa tatcttgaag cttcaaaaaa
     1321 cggaaaaaca tggttagcag aattacaagc caaagaaaga caacgtacag gaataaaatc
     1381 attgaaaata agctttaata aagtgtttgg ttattttata gaaataacac gtgccaactt
     1441 gcaaaatttt gaaccaagtg aatttggtta tatgaggaag caaacgttat cgaatgctga
     1501 acgttttata actgatgaac ttaaagaaaa agaagatatc attttaggtg cggaagacaa
     1561 agccatcgaa ttagaatatc aattatttgt tcagctacgt gaagaagtta aaaaatatac
     1621 tgaacgttta caacaacaag ctaaaattat ttcagagcta gattgtttac agagctttgc
     1681 agaaattgct caaaaatata attacactag gccttcattt agtgaaaata aaacattaga
     1741 attagtggaa tctaggcacc cagtagtgga aagagtaatg gattataatg actatgtgcc
     1801 taataattgt cgattagata atgaaacatt tatatattta attacaggtc cgaatatgtc
     1861 tggtaaatcg acatatatga gacaagttgc cataattagt ataatggccc aaatgggagc
     1921 ttatgtccct tgtaaagagg cagtgttacc tatatttgat caaatattca ctagaatagg
     1981 tgcggcagat gatttggttt caggtaagag tacgtttatg gtagaaatgc tagaagcaca
     2041 aaaggcatta acttatgcaa cagaggatag tttgattatt ttcgatgaaa ttggacgtgg
     2101 tacttcaacg tatgacggtt tagctttagc gcaggcaatg atagagtatg tagctgaaac
     2161 atcacatgct aaaacgttat tttcaacaca ttatcatgaa ttgacaacat tagatcaagc
     2221 attaccaagt ctaaaaaatg ttcacgtcgc tgctaatgaa tataaaggtg aacttatatt
     2281 cttgcataaa gtcaaagatg gtgcagttga cgatagttat ggtattcaag ttgcgaaatt
     2341 agctgattta cctgaaaaag ttattagcag agcacaagtg attctaagcg agtttgaagc
     2401 gtctgctggt aaaaaatcat cgatatcaaa tttaaaaatg gtcgaaaatg aacctgaaat
     2461 taatcaagaa aatttaaact taagtgttga agaaacaact gatactttat ctcaaaaaga
     2521 ctttgaacaa gcatcatttg atttgtttga aaatgatcaa gaaagcgaga ttgaactaca
     2581 aattaaaaat ttgaatttat ctaatatgac accaattgag gcattggtga agttaagtga
     2641 attacaaaat caattaaaat agaggtgttg caaaatgggg aaaattaaag aactccaaac
     2701 ctcattagca aataaaatcg cagcaggtga agtagttgaa agaccgagtt ctgttgtgaa
     2761 agaactgttg gaaaatgcta tagatgcagg cgctacagaa ataagcattg aagtagagga
     2821 atctggcgtc caatctattc gcgtagtcga taatggaagc ggaattgaag cggaagactt
     2881 aggattagta tttcatagac atgcgactag taaattagat caagatgaag atttatttca
     2941 tattaggaca ttaggattcc gtggtgaagc actagccagt atttcatcag ttgctaaagt
     3001 aacattaaag acttgcacgg ataatgctaa tggaaatgaa atatatgtag aaaatggtga
     3061 aatattaaat cataagcctg caaaagcgaa aaaaggaaca gatatacttg tagaatcatt
     3121 attttataat acaccagcac gtttaaaata tattaaaagt ttatacactg aactaggtaa
     3181 aataacagat attgtcaaca gaatggcaat gagccatccg gacattcgaa tagcactcat
     3241 ttcagatggc aaaacaatgt taagtacaaa tggttcagga cgaactaatg aagtgatggc
     3301 agagatttat gggatgaaag ttgcacgaga tttagtacat atatctggag atacaagtga
     3361 ttatcacatt gaaggttttg ttgcaaagcc tgaacattct agaagtaata agcactatat
     3421 ttctattttt attaatggac gatacattaa aaactttatg ctaaataaag cgattttaga
     3481 aggctatcat acactcttaa caataggtag gttcccgatt tgttatatta atattgaaat
     3541 ggatccaatc ttagtagacg taaatgttca tccaacaaaa ctagaagtgc gtttatcaaa
     3601 agaagagcaa ctatatcaat tgatagtgag caaaatacaa gaagcattta aagaccgtat
     3661 attaattcct aaaaataact tggattatgt gccgaaaaaa aataaagtgt tacattcatt
     3721 cgaacaacaa aaaatcgaat ttgaacaaag acaaaacaca gagaataatc aagagaagac
     3781 gttttcatct gaagaaagta acagtaagcc atttatggta gaaaatcaaa acgatgaaat
     3841 agttataaga gaagattcat ataatccatt cgtaacgaaa acgtctgaaa gtttaatagc
     3901 tgatgatgaa tcttccggtt ataataatac acgtgaaaaa gatgaagact acttcaaaaa
     3961 gcaacaagaa attctacaag aaatggatca aacatttgat tcgaatgacg atacatctgt
     4021 gcaaaattat gagaataaag cgtctgatga ttattatgat gtaaacgata ttaaaggaac
     4081 aaaaagtaaa gaccctaaac gaagaattcc atatatggaa attgttggcc aagtacatgg
     4141 aacgtatatt attgctcaaa atgaatttgg catgtacatg attgaccagc atgcagctca
     4201 agaaagaata aaatatgaat attttcgaga taaaataggt gaagttacca atgaagtaca
     4261 agatttatta atcccgttaa catttcattt ttcaaaagat gaacaattag tcattgatca
     4321 atataaaaat gagcttcaac aagtaggtat catgttagaa cattttggtg gtcatgatta
     4381 tattgtaagt agctatccag tttggttccc taaagatgaa gtagaagaaa ttattaaaga
     4441 tatgattgag ctaattttgg aagagaaaaa agtagatatc aaaaaattac gtgaagatgt
     4501 agcaatcatg atgtcatgta aaaaatctat taaagcgaat cattatttac aaaaacatga
     4561 aatgtctgat ttaattgatc aattaagaga agcggaagat ccatttacat gtccacatgg
     4621 tcgtccaatt atcattaatt tttcaaaata cgaattagaa aaattattta agcgtgtgat
     4681 gtagagagga tgaatcaagt gaataacaac atattgcctg ccataagaaa cattaaagat
     4741 ttagagaaac tgattaaaac agattataaa atgtgtgtgc ttctagatat gcatatagga
     4801 catataaaaa gtattatgga attgctgaag caaaatcata tagagtgttt tattcatata
     4861 gatttgataa aaggtttaag ccacgatgaa tttgcaagtg aatttattat tcagcaatac
     4921 aagccaaaag gtatcgtatc gactaaatct aaagtaataa aaaaagctaa atcattaaat
     4981 actttaacga tttttagagt atttattatt gatagtcaag cattgaaacg cagtatagat
     5041 ttgataaaaa aagttgaacc tgattttgtt gaagtacttc caggtgttgc gagtaaagcg
     5101 attcatcata ttcagaaaga aacaaacaca caagtcattg caggtggcct aattaataca
     5161 atagatgaag tcaatgaagc tgttaaaaat ggagcgaaat atgtaacaac tagttatgat
     5221 aaactttggt aaatcataag tctgcaaaaa tttcattttt cacgagattt agtcatataa
     5281 catattaaaa aagccgtttt catatggga
//
LOCUS       DAMZLE010000147         5279 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMZLE010000147 DAMZLE010000000
VERSION     DAMZLE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.150"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(41..901)
                     /locus_tag="POB92_002336"
     CDS             complement(41..901)
                     /locus_tag="POB92_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6579802.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            1014..1565
                     /locus_tag="POB92_002337"
     CDS             1014..1565
                     /locus_tag="POB92_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDI6579803.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLKVNFVNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPFDIL
                     NDSQFIEELANINLALKKGDIYD"
     gene            1558..2778
                     /locus_tag="POB92_002338"
     CDS             1558..2778
                     /locus_tag="POB92_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579804.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSFIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(2850..3146)
                     /locus_tag="POB92_002339"
     CDS             complement(2850..3146)
                     /locus_tag="POB92_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDI6579805.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(3195..3398)
                     /locus_tag="POB92_002340"
     CDS             complement(3195..3398)
                     /locus_tag="POB92_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579806.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            3707..4693
                     /locus_tag="POB92_002341"
     CDS             3707..4693
                     /locus_tag="POB92_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDI6579807.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
BASE COUNT         1803 a          821 c          813 g         1842 t
ORIGIN      
        1 atctttgagt ttcaccataa ctacacgttt tcaccaataa ttataaaaaa acatctttta
       61 caaaagattc aagtacttgc ttattcgatt caggattaat tatcaatgtt gtttctaaat
      121 tcggaatatc gacatctacg tctatttgaa acaaactttt attttctaat tcatctgcaa
      181 caataaattc cggcaatagc gcaatcccac ctgaattttt caagtgtttt tttaatgact
      241 caattcctag aaattcaatc atttgttttg ttgaaaagct atatttatat aaaatcattt
      301 ctaactgttc tctgtaacta cacccttttt cagtaacaag taatgtatgg ttttgaatat
      361 cgtttaattt gaaatctttc atctctttgt tagtaacaaa tattaaacgt tcttcagtta
      421 taggaatagc agttaagtta ctattaataa tcttttcgtc tgtgataagg ctaatatccg
      481 atttgaaatt agcaatttct tctttaatat agaaattaga atgtgcggat ttaaacttca
      541 agtttacata tgggtgttca gctaaccatt cactaatctt attatagtag cgatacaaat
      601 actgactttc ttgcatgtta atacttatat ttaccttctt attgtaaagt ccttttattt
      661 ctctctcgta ttcgctatta ctatctaaaa tcactttagc gtatttaacg tatgctaagc
      721 cttcttccgt tagttccata ccatattttt ttctataaaa taatttaaca tctaattgcc
      781 tttctaattt ttcaatatgt gtagatacag ttgactgtga atagttaagt aactctgatg
      841 ctttagtaag gttctttgtt tcactagtta ctaaaaatga ttttaaaatg tttaagttca
      901 aagtagtacc tcctttaggg ttccctttta tcgaatcctg aattctaaga attctgattt
      961 aataataata tattaaatat tattataaag gtaaatcgaa aggagttatg actatgatta
     1021 cttgtttatt tggaagtagt agaaaagatg gcaattcagc tatcgcagta gagaatctat
     1081 tgaagaattt aaaagtcaac tttgtgaatt tatatcaaag caacattgaa aaggttacag
     1141 ataatcgtca tagcaaaggt gccaactttc ataatgatga ttacgaacaa attttaatca
     1201 aagttttaga atcagatatt attatttttt caacaccact ctattggtac tccatgagtg
     1261 cgtctttaaa actcttcatt gatagatgga ccgagtcatt gcgagatacg caaatagaca
     1321 acttcaaaga aatcatgtca caaaaaaagt atttgatttt aatcataggt ggcgacaatc
     1381 cgcataccaa agcacaacct ttagtcaacc aattcaaatt aatatttgag tttatgaata
     1441 ttactaacta tagatttttg atcggcgaag gcaataaacc attcgatatt ttaaacgact
     1501 ctcaatttat agaagagctc gcaaatataa atcttgcatt gaaaaaaggt gatatttatg
     1561 actgatttgt tgaaaattaa aaacttcaga ttgttctttt tagcagaaat aatttcagca
     1621 ttcggagttg gaattagtac agttggggct aattggtatt taatcgacaa aactaacgac
     1681 tcccaactat taggtattat gttagcttta aatgtattat ccggtttctt agcttctcct
     1741 ataataggtg gactagccga taaatataat agacgaaata ttattttaat cacctatctt
     1801 cttcaggtaa tattatattt actcattgtc atagcactcg taatgatagg atttgagacc
     1861 tatttagtga ttggatttgc gattgttaat gggataggtt ggactacgta tatggcaaca
     1921 tctagaagtt tagttaaaca gatattaaaa ccagatcaat atacagatgc aaattctcta
     1981 ttagagatta gtttgcaaac aggtatgttt attgcaggtg gattatcagg aatattatat
     2041 aaaataaatg gattcactct aattatagcg atgactataa tgatgtttct aatcagcatt
     2101 tttatgttat ttagattgca tgtagataaa ccaactcatt cagaggaaga atcaacaaat
     2161 agcttattgc aagaatattt attaggatgg aaatttttaa aagacaacat gatgatattt
     2221 atttttggag ttatttcaat cataccaatg gtgtttacaa tgatctttaa catatcatta
     2281 ccaggatatg tttataatgt tttaaaactt tcgtctgtac aatttggttt ttcagatatg
     2341 ttatatggca ttggaggatt atgtgcaggt ttaatttcag ctattctttc gaagaaaatc
     2401 tcaactaaag tattgatatt tttgttatat ttcatattag tcataaattc agcactattt
     2461 atttggataa actcagcatt ttacttattc ataggatcat ttatactagg ctactcaatt
     2521 tcatcaatca gaatttatat gaatacagct ataatgaaca ctgtttcaga taaatatgtc
     2581 ggtcgctcat ttacgatatg gacgtcaatt tcattgttac tgcaaagttt cattgctcca
     2641 tttttaggaa gatggattaa tgaaattaat gataaattcg gtttctatat tatactcatt
     2701 ttatccttac tcatatttgt tacactgctg cttgttaaca aaacagacaa aataaaatat
     2761 gcacataaag aagagtgacc gtcactcttc tttaacaagc gaccatttat cgatgggctt
     2821 agttctctct gcacccacac ttcactactt cactttttca aatcattttt tatggtctta
     2881 aataaatcag tgagatttgt tgcttcggta aagtctagaa ttaatatcat ttctttagaa
     2941 cctggatatg gcgaaactaa tgtattatct tgcaatttct gctgggcact tttagtcgcc
     3001 ttgaccaata atctattatc atacaaacca cctataacca cgccatcata ataaataata
     3061 tattctccca tcatctttct tgtcttaacc gcgtttgaat tcacatgatt taaaaataaa
     3121 tcatgtacat ctttcttagt cgccattgta atcgctcctt cagttttatg tttaatcaca
     3181 ttcgtattaa tgattcattt cgtgttgctc ttaattttat ctataattat atgcgtagtt
     3241 aaaatcaaac ctatggaaaa gaaaataatg ataacaatgt taagaaatat agttataaaa
     3301 ttatagtttg gaaggtatgc gaatagcaga aaaataggta tcgcaaaaaa taaatcccac
     3361 caacctaaac tttttaaaga atgctttaaa ccttccataa tatcaccttt ataaatttgt
     3421 ctttgttata agataactaa aaaatcgctt tactgtaaaa gtagccaaag aaaattctga
     3481 atcatattca taagtagtgt atcattaata atgaacaatt taatactata atccttgatc
     3541 tttgtattga tcaacttacc acaacattta ttttagacta ctcttagact tccctttcaa
     3601 atggttgcat ctattgaaat tccttttgta taagttaggc ttttgtggta atatcatcat
     3661 gcataaaaaa tcgagatact aattataaag agggtataaa tatattatga aagaaaattt
     3721 ttggagtgaa ttaccacgtc cattttttat tttggcgcca atggaagacg ttacagatat
     3781 cgtctttcga cacgttgtaa gtgaagcagc tagaccggat gtgtttttca ctgaatttac
     3841 aaatactgaa agcttttgcc accctgaagg catacatagt gtgcgcggac gcttaacttt
     3901 tagtgaagat gaacagccga tggtcgctca tatatgggga gataagccag aacagttccg
     3961 tgaaacgagt attcaattag ctaaaatggg ctttaaaggc atagacttaa atatgggatg
     4021 tcctgtagca aatgttgcta aaaagggtaa gggttccggc ttaatcttaa gacctgacgt
     4081 tgctgccgaa attattcaag cgactaaagc aggtgggctt ccggtaagtg ttaaaacacg
     4141 ccttggctac tatgaaatcg atgaatggaa agattggttg aagcacgtct tcgaacaaga
     4201 cattgccaat ttatctattc atcttcgtac acgtaaagaa atgagtaaag tagatgcaca
     4261 ttgggaatta atcgaagcta ttaaaaattt acgtgacgaa attgcaccaa atacattgtt
     4321 aacaattaac ggtgatattc ccgatagaaa aacaggactt gaactggcag aaaaatatgg
     4381 cattgatggc gtcatgattg gtagaggcat tttccacaat ccattcgctt ttgaaaaaga
     4441 accacgtgaa cacacaagca aagaactatt agatctgttg agattgcatc tatcattgtt
     4501 taacaaatat gagaaagatg aaatacgaca attcaagagc ttgcgtagat tctttaaaat
     4561 ctatgtgcgt ggcataagag gcgctagcga acttcgccat caattgatga acacacaatc
     4621 aattgcagaa gcaagagcac ttctagatga atttgaagcc caaatggacg aagacgttaa
     4681 aattgaatta tagtatgagt aataaagttt atggatgata tttcccaatt taacacggat
     4741 tgaacacttt ctacttaggt attatcttgg ttttcctgat aggataactc ccgatgatgc
     4801 ctttttactt atgtcactta tccttttgga aatggtgcaa aattaaaaat acatcaggat
     4861 atcgtttaac tcagaaaaaa actccaatca aaaaaaatgt ttacaacaca ttatgaaatt
     4921 aaaattgctc ttagtgttca acagacaaag ttattgaaat tactattgga ggtacatacc
     4981 aagctatcat tttgagtaca cgtggtttga atatctggca agaaatggaa attggtaatg
     5041 tcagtcataa agtcgctaaa cgtgctcaaa taccattaat tatttttaaa taatttagag
     5101 gatgaggtat aaacctttaa aaaacagcag tgagatgatt ttcaattaga aaatatctta
     5161 ctgctgtttc tattttcata atcattctta ttgaatgact tcatcgtgat catttaaaac
     5221 agtaacaact ttttcaatct tatactattt agagctaaaa attcacgaca tataaacac
//
LOCUS       DAMZLE010000148         5264 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMZLE010000148 DAMZLE010000000
VERSION     DAMZLE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.151"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(175..798)
                     /locus_tag="POB92_002342"
     CDS             complement(175..798)
                     /locus_tag="POB92_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6579808.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            complement(820..1932)
                     /locus_tag="POB92_002343"
     CDS             complement(820..1932)
                     /locus_tag="POB92_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDI6579809.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLIHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGRVTFHSQPTKGTQIQFTIPIK"
     gene            2095..2916
                     /locus_tag="POB92_002344"
     CDS             2095..2916
                     /locus_tag="POB92_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6579810.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            complement(3377..4762)
                     /gene="fumC"
                     /locus_tag="POB92_002345"
     CDS             complement(3377..4762)
                     /gene="fumC"
                     /locus_tag="POB92_002345"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDI6579811.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(4958..>5264)
                     /locus_tag="POB92_002346"
     CDS             complement(4958..>5264)
                     /locus_tag="POB92_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579812.1"
                     /translation="LYLPVIFKVIKLQLNLSKGLIDDVNAQTFIKSNQSGIVISVICC
                     LLITGILYYTLDGFYASLTGVLGTLVALNPYTTIVSAVLYILTAIATVEATKTKYRN"
BASE COUNT         1677 a          920 c          760 g         1907 t
ORIGIN      
        1 gaaagtaaat gaagtaaaat tttatgcata atcactagat ttgataaaaa ttacactttt
       61 ataatgatat caaatttaca aagaaaactg tataataaac cctatcgcta taaaaaattc
      121 atcaaaatca gattaaaaat tgtctatttt tcgacaaata tttaaaagct cattctaaat
      181 caacttattt tccattgcat aaattgctgc ttgtgtacga tcgctaactt gtaatttact
      241 aaatatatga ctgacatgtg ttttaattgt tttttcagat acaaataaag tttctgcaat
      301 ctctttattt gttttacctt taaccatttc acgtaacact tcaatttctc tctttgacaa
      361 cttattcgtg tagtgtggtt tttggctaac tgtttcgaat acatcttgtg ccttaggatg
      421 tatcattttt tcaccgttca taactcgtct aatagtttca attaattgct gaggctcaac
      481 gtctttcatt tcataaccat cagcaccttt attgattgct gaaattacat gttcatcatc
      541 aacataactt gttaatacca aaactttaat atccggataa tgtgccttaa tatattccgt
      601 aatttcaata ccattcatgc caggcatcac taaatctaat agcacaatat cagggtgctc
      661 atgctctttt aaatattcta aaaatgtttc tccatctgca aagtcttgta aaacttctat
      721 gttttcaatc gtggataata aaaatcgcaa tccttgtcgc acaatataat ggtcatctac
      781 taatattact ttgttcatgg gttatctcct taaatcaagc tattttatag gaattgtgaa
      841 ttgtatttgt gtaccctttg ttggctgaga atgaaaggtc actctacctc ttaataattt
      901 aactctttgt tttatgttat taataccgtg tgatgaagct atctgaacat tatcgatctc
      961 aaatccttga ccataatcaa tcacgtcaat atatagtata tcgttcattt gttttaatgt
     1021 aagatccatt ttattcgtat cagcatgttt cttaacatta ttaatacact cttgtaatgc
     1081 tctatatatg ttttcttcga tttcattaga taaatcgatt aaaccttcta catttacatt
     1141 taattgtata tggattaatt tactatatgc tgtcaaagca tgaattaaac cttgctcaag
     1201 tccaactggc ttaagttgcc aaatcaatgc acgcatttca ttaacggcat tttgactcgt
     1261 ttcctcaatc gtcttgaatg cttgtttagc gatggattcg tttgacatgc catacgcagc
     1321 atgtgctgtt agttttacag aaaataacat ttgatttact gaatcatgta aatctctagc
     1381 taaacgatta cgttcattaa tttttgctgc ttctttttca cggtctgtta aataaatacg
     1441 tttgatggct gaccctaatt gaaatgcgac agactctagc aactctaaat cttcatcact
     1501 atatatttca gtatttggtg acgctacatt taaaatgccg aattgttctt gacccgattt
     1561 aagtggtacc gttgcatgat gtgtaatatt gtcattttgg ctaggaaatg ctttagaggc
     1621 taagttaata cgagaacaat tgacgatatt cgacgctttc attagcctac gttgattaaa
     1681 tgctttcaca caccaacaag acccatcttt aatatagtga cagtggtctg ctgtcaaaga
     1741 ttgtggtaga gccacatgtg atacaagttc atgttcacct acgctattga tgaaaaatat
     1801 ccagcctgtc gtgaaattac tgccctcaat taaatatttt aacgcacctt gggtcatgct
     1861 atacatttca gtttcttcgt ttaaaaattc ggcaatctct tttaataaag ctagtcgcgt
     1921 cctttgttcc atcaaatcgc tccaattcat ttttacgtgt attaactatt atacattgag
     1981 ttattatatt tttaaatctt agacgtgaac atgataaaat ggccttgatt actcaatagt
     2041 tatatttcgg agaactgatt tgtgatatga tattaaagac tataggagga ttttatgaaa
     2101 tttaaaatac cagaaaactt taatgactta agtttacgag atattttcca acaacttaag
     2161 gtacctaaaa aagatttaca tcatttaaat atgtctaaag atattactat taatgataaa
     2221 cctgcgcgat taatggataa agtgcatact ggcgacgatg tatttgttcc aaccatcgat
     2281 gaaaaaagta attatgttcc aagttatcgt tatgcacaaa ttaaatacga agacgatgat
     2341 atggcaatcg taatgaaacc taaaggtgtt aagactcacc ctaatgattt aaaagaaagc
     2401 aatactttaa tgaatcatgt gatttacact attgatagtg actatgtcga accaattcat
     2461 cgactggacc aggaaacagt aggattatta attgttgcta aaaatccttt aatgaaaaaa
     2521 attcttgatc gcatgttaga agacaatgat attacgcgga tatacaaagc aaatgttaag
     2581 gcacttttac ctttgaaacc acaaacgatt gatatgccaa ttggtaaaga taaattccat
     2641 tcgaataaac gacgtgtgtc tcctactgga cagcgtgcaa ttacacacat tttaacttca
     2701 aaaatgataa aagaagctgt gtgccaactt gaaatcaagt tggatactgg acgtactcat
     2761 caaattcgtg tgcatttagc tgaaattggt caccctgtta ttggtgatcc tttatatggt
     2821 gattcaacgt taagacaatt agaacttgaa agttacaaaa tagagtttgt gcatcccttg
     2881 actaaggaag tcatttccgt ttctttggat gactaatttg attagttttg catgatattt
     2941 aaacatgcaa taccgcattg taactaaatc aagtatcaac ttaaatcgga tagatggaaa
     3001 attattaatt ttttcagatg ttcggttttt tgttttttac gatgcttagg attttatatt
     3061 ttgatatttt agtaattatt cattttataa catccttgga taataacttg tagtcttttt
     3121 caactgcgtt acgtgtatct atggacaata catgacatca taagattttt atcacaggtt
     3181 gtttggccaa tacatgtaca acaattcatc atataaaaaa taggttctat aataaaacgg
     3241 actccatgaa aagttttttc tttcacggct ctatatcaaa tcagacttat aagttcaatt
     3301 tgacatagac aaaaaataaa gcttgaacac tacatctacc tgtaatgccc aaactttatt
     3361 ttatattaat atttaattaa tgaggatcta ccatatcttc tggtttaatc catgcttcaa
     3421 attgttcttc tgtaacatat ccagtttgaa ttgcagattc ttttaaagtt aaaccttctt
     3481 tatgggcttt cttagcaatt tgagctgctt tttcataacc aatatgtgga tttaatgcag
     3541 taactaacat taatgattga tttaaataat tatcaatatt ctcttcgatt ggttcaatgc
     3601 ccactgcgca attgttatta aatgtttcca taccatcagc taaaaggtaa attgattgta
     3661 gtgtattatg cataataact ggtttataaa cattcaattc aaagttacct tgtgaacttg
     3721 cgaagccaac aactgtatca ttacccatta cttggactgc aaccattgtt aacatttcac
     3781 attgtgtagg attaacttta ccaggcataa ttgatgaacc tggttcattt tcagggatag
     3841 aaatttctgc caaaccagct cgtggccctg aagccaacca tctcacatca ttagcaattt
     3901 tcattaagtc tcctgctagc gccttcaatg ttccatgtag ttgtactact tcatcatgtg
     3961 ctgttaatgc atggaattta ttttcagaag atacaaatgg ataacccgta ttttctgaaa
     4021 tataatgtgc cactttatca ccaaattcag gatgcgcatt aataccagta ccaacagccg
     4081 taccaccgat ggcaagattt aaaatgtgct tcttagattc agataacatt atttcgcaac
     4141 ggtcaagcat ataacgccag ccactaatct cttgtcctag tttgatcggc gttgcatctt
     4201 gtaaatgtgt acgaccaatt ttaataattg aatcaaattt gtcttctttt tctttcaaag
     4261 tatttcttaa aagttttaat gcaggttcta attttgtttc aacctcttga tataatgcaa
     4321 cgtgcatagc agttgggaat gtatcattcg aactttgtga tttatttaca tcatcatttg
     4381 ggtggatact ttcatcaatt tgatgatctt ttaaatacat attagcaaca taacttacta
     4441 cttcgttcac attcatatta ctttgtgtac cgcttcctgt ttgccataca actagtggga
     4501 agtgttcatc taattcacct gataaaattt gatcacatgc gtatacaatg gcatctttct
     4561 ttgcctcgct taattttcct aaatcaaaat tagctaatgc tgctgcacgc tttagttgtg
     4621 caaaaccata aactacttcg attggcatac gctctttacc aactgggaaa ttgcgtttac
     4681 ttctttctgt ttgagcaccc caatatttat ctgcaggtac ttctatttct ccaaaagtat
     4741 catgttcaat tcttactgac attcaatttc tccccttatc actgtttatt taactgtagt
     4801 atatcattaa ataatttaat tgagcaattt atgattaaaa cgttttcata atttgaaata
     4861 aaaatacact aatcgcacgt actcaccctt tattacagtg atacggtcat acgattagtg
     4921 tgttatctat cattatttag ttattgttga actaagttta attacgatac tttgttttag
     4981 tagcttcaac cgtagcaata gctgtaagta tatataatac agcactaaca attgtcgtat
     5041 atggatttag agcaacaagc gtacctaaaa ctcctgttaa actcgcataa aacccatcaa
     5101 gtgtataata caatattcct gttattaata aacaacaaat tacacttatt acaatacctg
     5161 attgattact tttaatgaac gtttgcgcat taacatcatc gattaatcct tttgataaat
     5221 tgagttgtaa ttttattact ttgaaaataa caggtaaata taat
//
LOCUS       DAMZLE010000149         5233 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMZLE010000149 DAMZLE010000000
VERSION     DAMZLE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.152"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(75..259)
                     /locus_tag="POB92_002347"
                     /pseudo
     CDS             complement(75..259)
                     /locus_tag="POB92_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(348..1412)
                     /locus_tag="POB92_002348"
     CDS             complement(348..1412)
                     /locus_tag="POB92_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDI6579813.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="POB92_002349"
     CDS             complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="POB92_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDI6579814.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="POB92_002350"
     CDS             complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="POB92_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDI6579815.1"
                     /translation="MIVNYLKHKFYNLLTTMVVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(3897..>5233)
                     /locus_tag="POB92_002351"
     CDS             complement(3897..>5233)
                     /locus_tag="POB92_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579816.1"
                     /translation="KSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIF
                     TAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQG
                     FGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLF
                     YINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLL
                     NFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIP
                     VYLQEQLGLSPLQSGLVIFPLSLAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSII
                     ISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGAS
                     IGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT         1872 a          978 c          669 g         1714 t
ORIGIN      
        1 atgttcaatc tcttttattc tacttcattt atttcttgaa gtcaataact ttttgaacga
       61 ttactttatt tattctatat tgtttttata gttatttcaa tggtaagttt tacacttttg
      121 aaattcttct ttaaaaacaa ctgcgtcgtt tttgacgact ttatcatatt atcaactttg
      181 gaatttaaag tcaataactt ttttaaaact ttttgtgttc acaacccgct tctttttcaa
      241 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      301 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      361 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      421 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      481 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      541 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      601 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      661 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      721 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      781 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      841 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      901 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
      961 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1021 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1081 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1141 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1201 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1261 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1321 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1381 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1441 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1501 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1561 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1621 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1681 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1741 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1801 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1861 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1921 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     1981 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2041 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2101 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2161 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2221 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2281 ataaccatac ttattatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2341 gcactactaa atgcgtatag cattaacccg aatccaatag ttgaatacat tactgagcgc
     2401 ttatctaatt tcgtatattt cacttcattg taacttgaga ttgaaactaa gccgaaaaca
     2461 aaggcaacta aagcaattcc tacaattata ataaatggta ctcgccaact cgcttgatca
     2521 ataattaatc ctgtaactgt agggcctatt gctggtgcaa attgaattac taaacctgct
     2581 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2641 aagggcataa gtacgcctgc acccatcgct tgaattacac gtgctaacat taaaactcca
     2701 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2761 tataaaggtc tagttttaat tctatccatt agatatgccg tcaaaggtat catgacgcca
     2821 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattaat atttaattct
     2881 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     2941 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3001 atcataattc ctccttttta aaacagatat acacttgtcc ctttaataca tatagccatt
     3061 aacataatgg gagtagtact caaatgataa agaagcacta cataataaat cattattggt
     3121 tcttacacat taggcacagc taatgtgtac ttaaaaaact aacaatataa cattgtaggg
     3181 cctaataatt tattttattt ctcagactca tacaatggtt atttaactta tttaccacaa
     3241 attgagcact aagactcact attatcgatt cttctattga acaaaaagaa gttggaacac
     3301 atatgtgccc tcaacttcag atatctattt tctattattt aaatttttac tttcagggta
     3361 aaattgaagc ttacaaacat ataagaaccc tataataacc atgatgaccc aaaaatcgat
     3421 attgatatac acatagttca gatgaataat aaaataaaat aacgttgttg caactgtgta
     3481 agaaatcaaa tgaaaagtcg cgcctctaaa atatgcattt tcaggcaaca tttttctaaa
     3541 ataatccatt aaatgctcga ctgcaaatat gatgaagtac ccaaagatta tataactgcc
     3601 ataatataat aggttatcgt acatacttct attatatgta aagtcaccca acctaaaata
     3661 aataagtatt ctacttaatc catataaacc aaaccctaga aatgttaaaa aaatcgcacc
     3721 agaaagtaca aaaataaaga gaacaaccat tgtagttaac aagttataaa acttatgttt
     3781 aagataattc acaatcattg gcctccttat taataaaata ttgaacgatt tatctagtat
     3841 ctaggaaatt tattatttct tactttaatt acactacatc aattttaatc ataattctat
     3901 tcttttgatt gagatgactg ttctttaaaa aatacgaccc ataaaacaat aagcccaatg
     3961 ctaattacag caacagcact taacacatta tgtaagtttg gaccaaagat tcctgattgt
     4021 atcgcataaa gatatcccat tattgttgaa cctattgatg ccccaagatt tttagttaat
     4081 ccataaaatg acatcatttt cttcatttgt aatgggctag tttcctcttg gacaattaca
     4141 ctatctttcg tataaatata tccaaaactt aatcctgcca aaatcaacac aaatgctata
     4201 agtaccggca atttaatacc aaatgaaatg ataatactac ttactagtag caatgtaaat
     4261 gatagtaagt aaataacttt ccttgatagt tttgcttcaa ttcgatgtaa attaaaattc
     4321 aatgtaatcc aagctaaaga taaaggaaaa ataaccaatc cactttgcaa tggagataat
     4381 cctagttgtt cttgaaggta gactggaata tataaattga atcccattaa acaaatagct
     4441 gttagaaggt cagttataaa aactaaagta atcgaacgat taaattcaac cacaggtaaa
     4501 aatggtgaag aaacatgttt ttctacttta aataaacaca ttgccacaac gatagctaag
     4561 ataaaactca agaaatttaa aagtaacagc tgttggttta ataaagcaaa cataatcaat
     4621 ccaataaata cataaaatag cgtgagccct ttagtatcaa attttgattt agcaactgtt
     4681 tcttcctctg gaaaatgaaa tgtccatata actaatataa tggcaatgat cgcaatcggt
     4741 atatttatgt agaataacca atgccatgtt gcaaattcta atataccacc acctagaagt
     4801 ggcccaatga tactcgaaat accccaaaca ctgccaacta tgcccataat tttatatcgt
     4861 aacggaattt caaatgctaa ctttggaaca atttgcgata atgacatcaa tacacctgat
     4921 ccaaaacctt gtatcacgcg tgatataatt agcatagtaa aattagtact taaaccacac
     4981 ataaaactac ctatactaaa taaaagtaat cctgcaattg ccacataaat aattttaaat
     5041 ctcgaaagta attcgcccac tataggattg gcaattacta atgcgataaa atacgctgtg
     5101 aaaattaatg aaattaaatt tcctgcattt aaatcttgtt taattgtagg taatgcaagc
     5161 gaaataatag atgattcaat cgctgacata aacataatta atattaaagc tataacagtt
     5221 atgattgact tta
//
LOCUS       DAMZLE010000150         5232 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMZLE010000150 DAMZLE010000000
VERSION     DAMZLE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.153"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(40..687)
                     /locus_tag="POB92_002352"
     CDS             complement(40..687)
                     /locus_tag="POB92_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6579817.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(800..1348)
                     /gene="hxlB"
                     /locus_tag="POB92_002353"
     CDS             complement(800..1348)
                     /gene="hxlB"
                     /locus_tag="POB92_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDI6579818.1"
                     /translation="MAEFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1350..1982)
                     /gene="hxlA"
                     /locus_tag="POB92_002354"
     CDS             complement(1350..1982)
                     /gene="hxlA"
                     /locus_tag="POB92_002354"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDI6579819.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2059..2817)
                     /gene="nagB"
                     /locus_tag="POB92_002355"
     CDS             complement(2059..2817)
                     /gene="nagB"
                     /locus_tag="POB92_002355"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI6579820.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3093..3455
                     /locus_tag="POB92_002356"
     CDS             3093..3455
                     /locus_tag="POB92_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDI6579821.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            3469..4134
                     /gene="bshB2"
                     /locus_tag="POB92_002357"
     CDS             3469..4134
                     /gene="bshB2"
                     /locus_tag="POB92_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDI6579822.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            4147..5025
                     /locus_tag="POB92_002358"
     CDS             4147..5025
                     /locus_tag="POB92_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDI6579823.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT         1682 a          949 c          823 g         1778 t
ORIGIN      
        1 tatgcatttt aaccatggta ttctctgttt taacaaattt tataatactg tattaatttc
       61 tgatggatca ttgattataa aatccggatt tgaatgaagt aatgaccttg catcatgtgc
      121 tccccatgtt actgctgcag atggtacacc agcatgttgc gccatctcaa catcaaatgt
      181 tgaatcacca atataaaccg tttgttggct atttaaattg tagcgttgca caattgtgtg
      241 tatgccttca ggatttggtt tatatgcact tacttgatcg gatccaacag cttcggtaat
      301 caagtgattt aatccaatag ccgataaatt tctttctaat acatcactct tcttactaga
      361 caccacgaaa agttttttcc cttggttata caaacttgta atggcttcag ttatacccgc
      421 aaattcataa atatagtcct tttcaataga ttgatatgta tgtctaaatg tatcgattaa
      481 ctttgctaat gctgcttcat ctaatggtcg gtctgctaat tttaaaaatg attcttcaat
      541 aggtattccc atataatgcg ttatttcttt agatgatggt tccgttaagc cacatgcttt
      601 aaatgcactt tgtgttgcta cttcaccaca ttttttcgtg tctgccaacg taccatcaaa
      661 atcaaaaata taattgtcaa acttcaatat taacacccct attcgtttat tattgatatt
      721 atactataat ttattattta aataaaaaaa taatcgataa tatttctgcc taggcatcat
      781 attatcgact atctttattt tattctaaat tagcatgatt ttgttgcatc gtttgttccg
      841 taacattcat ttcagtcatc aatcccatta caacactatc taaaaataat tgagatgctt
      901 gttcaaacaa acttcctaat ggttgtgccg agccttgttc atcatatttt gtacctgcag
      961 gcaaaacgat gttcgtccca gctagattgc ctattgcaga atctttattt gtagtaatta
     1021 atacgatgtc agcacctact gattttgctt tgtctgctaa taatcttaaa tgttccgtgg
     1081 aacctgaacc agagataatt acaaatacat cattcgactt aatcgcaggt gtcgttgatt
     1141 ctccaacaac atgtgcctgt ttgccgagct gatttaagcg cattgcaaaa ctattcgcca
     1201 cgaatcctga acgtccttta ccagctacaa atatatgttc agcatgtagt attttggatg
     1261 caaacgttga aaactcatcc gcttcaacat gtgacaaagt catctttaat tcatctagaa
     1321 ttaattgata gtcactaaat tcagccatat tacttacctt cgattgcagc gcgacattgt
     1381 tttgcagctt ctactggatc atctgcattt gcgattccgc caccaacaat aacaagatca
     1441 ggactttcag cgacaatttc tttaattgta tctggtttaa ttccacctgc tactgcaact
     1501 ttagaatttt taataacaga tttaacggtt cttaaacttt ctaatggtga ttgcccttct
     1561 gcttgtaaat cataaccagt gtgtactgca atataatccg cacccatttc atctagttct
     1621 tttgcacgtt tttctaaatc ttgaacagca atcatatcaa ctagtaattg tttattattt
     1681 ttatgagctt cttcaatagc tgctttaatt gatgcatctt ctgcaacacc tagtattgta
     1741 attacatccg cgccaaattt aattgcttgg ctaacttcat aatcagctgc atccataatt
     1801 ttcatgtctg ctaatacttt tacattacta atgttgtctg ccatatgttt aactgctggt
     1861 aaaccttcgt tgtaaatgat tggcgtaccg atttctacga tatctacata atcttttact
     1921 ttatttgcta actcagccgc gtcttctttg tttaataaat caattgctaa ttgtaattcc
     1981 actaaaaatc atccttccaa tgctttttat ctttctattg gaacctttcc cgaggcattt
     2041 cttaattaaa cattgcaatc aatccatttc atcgacatga attttcgcaa catttttcgg
     2101 gcaagcttcg tcgtctaaat atatctcaac attcgggtgt ttgtgaagta atgtggctgg
     2161 aacatcaaca gaaatttcct gatttaataa atgtgtaata gcagcacgtt tcttttcacc
     2221 aaatgcgagt aaaatgatac gtttggcttg aagaatatta gcaagtccca tcgaaatggc
     2281 ttgctttgga acatcatctt cgtttttaaa atatcgacta ttagccttaa tagtactttc
     2341 agtcaaatca acgatatgag taacgctgtc aaacggcgta ccaggttcat taaatccaat
     2401 atgaccattt tctccaatac ctaaaatttg aatatcacgt tgaccttgtt gttctaaaac
     2461 gtcattatat tttgacgctt ccgcattcat atcatcggca tctccatttg gaatatgaat
     2521 gttctttcta ttaaaataag gatattgttt gaaaagcatg tcatccatat aatagtgata
     2581 actttgcgga tgtgatgcgg ttaaacctac atattcgtct aaattaaacg tggatacatt
     2641 gtctacgttt aactgatttt tatttaataa cttaacaagt tgctcataca aatctgtcat
     2701 tgtaccacca gttgctaaac caagtttaca gtgctgatta aatgccatct ctttatataa
     2761 ctcacatgca acatagaatg atgcttgttt tttcgatcct aagtttaata ctttcattgt
     2821 ttacatgcct ccacaaattt aatacatttt aaaaacttat gaattcattc atacaccatt
     2881 aatatagcat catttttaat gtttaacact tttcaaattc caacaaattt ttgattttta
     2941 aaagtaaaaa actaatgctg tatgattgcg taatcctgag taattaccct tcatgaaaat
     3001 taatttataa ataattgagc aatcgtatac aattattgaa tagtttttag taaaatagaa
     3061 agataaacac attgatagtg taaggagtga acgtgttgga accaattaaa gaacaagaag
     3121 ttatagattt acttgcttct tttgaacata aacctgtgta tctgcatgtt gaaacgacaa
     3181 atggggccta tgcgaaccat ttcgatcaac gtgtttttaa tgctggtaca tttttaagaa
     3241 acatacaagt aacatatacg catgcacaac taaaaggtgg taataaagaa ccttatcgca
     3301 ttggtcttaa actatcaaat ggtggttggg tttatgtaca aggtctaacg cattttgagg
     3361 tcaatgaaca tgatgaattt ttaattgcag gttttaatta cgaaggtcaa ctcgctgcag
     3421 cacttcaaat tagtgagcga ccatttaatt tatagaggag gcatcaacat gactgacgaa
     3481 agacatgtat tagtaatatt tccacatcct gatgatgaaa ccttctcatc tgcaggcaca
     3541 ttagcaagtt atatccaaaa aggcatacct gtaacatatg catgtttaac cctaggacaa
     3601 atgggcagaa atttaggtaa tcccccattt gcaacacgcg aatcattacc aagtattcgt
     3661 gagcgtgaac tagaagaagc ttgtaaagtt attggtatta ctgatttaag aaaaatgggt
     3721 ttacgtgaca aaactgtcga atttgaaccc tacgaacata tagatggtat gattaaatct
     3781 ttaatcgatg atacaaatcc atcattgatt atttcatttt atcctggata tgcagtacat
     3841 cctgatcacg aagcaacagc agacgctgta attcgaacgg ttgaacgcat gcctaaagaa
     3901 gaacgccctc gtttaacttt agttgcgttc agtaatgatg caaccgaagc tcttggagaa
     3961 cctgatattc aaaatgatat tacagatttt aaagaactta aaattaaggc atttgaagca
     4021 catgcttctc aaacaggtcc atttttaaaa caattagcaa gtcctgaaat cgatggtaaa
     4081 caacacagct ttttaacagt cgaaccatat tggacatatc actttaaatc ttaacggagg
     4141 aaaaaaatga ctgaatttga tttatcgaca cgagaaggtc gttggaaaca ctttggttcc
     4201 gtagatccta ttgaaggcac gaaacctact actaagaacg aaatgacaga cttacaaagt
     4261 acacataaag attttttatt tgaaatagaa gaagttggta ttaaaaactt agtgtaccct
     4321 gtacttgttg accagtatca aacagctggc acatttagtt tttcaacaag tttaacaaaa
     4381 gacgaaaaag gtattaatat gagccgtata attgaaagtg ttgaaaagca ctatgataat
     4441 ggtattgaac ttgaatttaa tacactttat caggtgttac gcactttaca aacgaatatg
     4501 aaacaaaatg cggcaggcgt tgatgtttct gggaaatggt tctttgatcg ctatagtcca
     4561 accacaaata ttaaagcagt tggtaatgcc gatgtcactt atggattagc aattgacggt
     4621 gataaagtta cacgtaaaga acttactatt gaagcaactg tcacaacgct atgcccttgt
     4681 tcaaaagaaa taagtgagta ttcagcgcac aaccaacgtg gtgttgtcac tgttaaaaca
     4741 tatattaata aagatcaaga catagttgat gactataaaa ataaaatttt agatgcaatg
     4801 gaagctaatg caagttcaat tctataccct attttaaaac gtcctgatga aaaacgtgtg
     4861 acagagcgtg catatgaaaa tccacgtttt gtcgaagact taattcgttt aattgccgcg
     4921 gacttagttg aatttgattg gttagacggt ttcgatatcg aatgtcgtaa tgaagagtct
     4981 atccatcaac atgatgcttt cgctaaatta aagtatcgca aataatatca tatgattaat
     5041 tcaaacgcgt aacagtagtt gcgcgttttt attatacctt ctagagttgt atatctcttt
     5101 tataaatctt ctcatttgat gtgcctgagt cataaatcaa tttcctaaac tctacaatga
     5161 tatattggtc gtagttgact gaataactac taccattata aatatcaaaa cttgttgttt
     5221 tataaataaa ca
//
LOCUS       DAMZLE010000151         5207 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMZLE010000151 DAMZLE010000000
VERSION     DAMZLE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.154"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            258..1355
                     /locus_tag="POB92_002359"
     CDS             258..1355
                     /locus_tag="POB92_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDI6579824.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQHALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKLDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            1560..3122
                     /locus_tag="POB92_002360"
     CDS             1560..3122
                     /locus_tag="POB92_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDI6579825.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFVIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3133..3240)
                     /locus_tag="POB92_002361"
     CDS             complement(3133..3240)
                     /locus_tag="POB92_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579826.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(3312..4106)
                     /locus_tag="POB92_002362"
     CDS             complement(3312..4106)
                     /locus_tag="POB92_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDI6579827.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSEKETTFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(4126..5202)
                     /locus_tag="POB92_002363"
     CDS             complement(4126..5202)
                     /locus_tag="POB92_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDI6579828.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
BASE COUNT         1752 a          775 c          941 g         1739 t
ORIGIN      
        1 aacttttttg gaagagcaaa tcacgcaatc aacaaataat ataagaagtt tttgcgatag
       61 ttttaaaata gctgtaatag aatactaaat gtgacaaact tagaactaat atcaagtgtt
      121 gatgttttga atataaaaat gctaatagat ggcatcacga gatttatgac tatctgttaa
      181 atcaacctaa cacgattata atttgaataa ttggttaata tatgagtaat tagaaaatag
      241 acaaaggatg acgatttatg tatatcaata tgaaagatta tgggttaaca ggcataaaca
      301 aaactaaaga tactcgagca atacaacatg cgttaaatcg tggaagatgt aaaccaacga
      361 cagtttatat accgaaaggg acgtatgata tttgcaaacc attaacgata tatggcaata
      421 caacactttt gttggataat gaaactattt tacgccgatg tcattctggt cctttattaa
      481 aaaatggtca tcgctttggt ttttatcgtg gttataatgg acacagtcat attcatatta
      541 aaggcggcaa gcttgatatg aatggcgtat cgtatcctta taacaataca gctatgtgca
      601 ttgggcatgc tgaagatatt caattaatag gtgtgactat taagaatgta gtgagtggtc
      661 atgcaattga tgcttgcggg attaatggac tctatattaa aagttgttca tttgaaggat
      721 ttatagacta tagtggcgaa cgcttttatt ctgaagcaat acaattagac attcaagtac
      781 ctggtgcttt tccaaaattc ggaacgacag atggtacgat aacgaaaaat gtcgttatcg
      841 aagattgtta ttttggacct tcagaattgc ccgaaatggg aagttggaat cgtgctattg
      901 gctcacatgc aagtagacat aatcgttact atgagaatat tcatattaga aataatatat
      961 ttgaagatat acaaggttat gcattaactc ccttgaagta taaagatgct ttcattatta
     1021 ataataagtt tattaactgt gagggtggta ttagatattt aggagttaga gatggtaaaa
     1081 atgcagcaga tgtgatgaca ggaaaagact taggttccca agcaggcata aatatgaata
     1141 taattggaaa tgaatttaaa ggatcaatgt ctaaagatgc gatacatgta cgtaattata
     1201 ataatgttaa acataaagat gtattaatcg ttgggaatac attcaataat tcgactcaat
     1261 caattcattt agaagatatt gatacagtgt ttttaagtcc tgttgaagcg ggtattcaag
     1321 ttactacaat caatgtagat gaaataaaaa agtaaaaagt ttcgcatgac attaggatta
     1381 agaatagtag ataatttttg aaagcgcatt cataaaacgg tataaatatg ctataataaa
     1441 cccaattatc tgatgaaagg ggtattttga cggtaatgat aatacaagat agacaacttt
     1501 ctatactcta atatagtgag ttgaagtagc ttgtcataat catcatgagg gggaaattta
     1561 tggcttattt caatcaacat caatcaatga tatcgaaaag gtatttaaca ttcttttcaa
     1621 aatcaaagaa aaagaaaccg tttagtgcgg gacaacttat tggactaata ttaggtccat
     1681 tacttttcct attaacatta ttattctttc atccacaaga cttaccttgg aaaggcgtat
     1741 atgtcttagc gattacttta tggattgcga cttggtggat tactgaagca attcctattg
     1801 cagcaacgag cttattacca attgtgttat taccattagg tcatatactt acaccagaac
     1861 aagtatcatc cgaatatggc aatgatatta tctttttgtt tttaggtgga tttattttgg
     1921 caattgcaat ggaaagatgg aatttacata cgagagtagc gttgacaatt attaatttga
     1981 ttggtgcaag cacttcaaaa atattacttg gattcatggt ggcaacagga ttcttatcta
     2041 tgtttgtatc gaacactgca gctgtaatga ttatgattcc gattggttta gcaattatta
     2101 aggaagcaca tgatttacaa gaagccaata cgaatcaaac aagtattcaa aagtttgaaa
     2161 aatctctagt tttagcaatt ggctatgcag gtacgattgg tggcttgggt acattaatcg
     2221 gaaccccgcc attaattatt ttaaaaggac aatatatgca acattttgga catgaaatta
     2281 gttttgctaa atggatgatt gtagggattc caacggtcat tgttttgtta ggtattactt
     2341 ggctctattt aagatatgtt gcgtttagac atgatttgaa atatttacct ggtggtcaga
     2401 cgttaattaa acaaaagtta gacgagcttg gcaaaatgaa gtatgaagaa aaggtagtac
     2461 aaactatctt tgtacttgcc agcttattat ggattacaag agagtttctt ctgaaaaaat
     2521 gggaagttac gtcatccgtt gcagatggta cgattgctat ttttatatca atattattat
     2581 ttgttattcc agctaaaaat actgaaaaac atcgccgtat cattgactgg gaagttgcaa
     2641 aagagctccc ttggggtgta ttaattttat ttggtggcgg tttagcatta gcgaaaggta
     2701 tttctgaaag tggtttagca aaatggttag gcgaacagtt gaaatcatta aatggtgtta
     2761 gtccgattct tattgtaatt gtcataacaa tctttgtctt atttttaact gaagtgacat
     2821 ctaatactgc aactgcaacg atgattttac cgattttagc aacgttgtct gttgctgttg
     2881 gagtgcatcc attactactt atggcacctg cagctatggc ggctaactgt gcatacatgt
     2941 taccagtagg gacaccaccg aatgcaatta tctttggttc aggtaaaata tctatcaaac
     3001 aaatggcatc agtaggattc tgggtaaact taatcagtgc aataattatt attttagtcg
     3061 tgtattatgt aatgcctata gttttaggta ttgatataaa tcaaccactg ccattgaaat
     3121 agtaattgca gattagaacg aaaaataaaa ggttacatta gcaattgctt gaacgagtgg
     3181 taatgaaacg tatacagcag catcgtgtaa aaacaataca aacaaaagaa agtcaaccaa
     3241 ggatggattc ctattttaat ccttggttga ctctttattt tatttaaatt gtagaaccta
     3301 gaaaataaag tttaattaaa agcaccaatc atttctactt tgaaatctaa ggtttctaaa
     3361 atagcaatga ctttctttat atcggttgta attgcagaat cagcctgaac gaaaaatcga
     3421 tacataccta attgtgtttt taaaggacga gactcaatcc aggataaatt aatattaaac
     3481 aaagcaaatg tattaagcac acttgctaac aacccaggtt tatcatgcat tggtgtaatt
     3541 aaaaacatca atgatgtcgc attttgatca aattgctgct gattttttat aactaaaaaa
     3601 cgtgtcacgt tatgtggata gtcttcaata tgtgtatcaa taggtgtaaa accataagct
     3661 tcgccactac ctaaaggtgc aattgctgca acgccatttt caattttagt caaactttga
     3721 attgtactgt cgacataatc atagtcaaat tgatgttgat gtatgtaatt tgttgtttga
     3781 ctgattgctg gtgcaataga atacactttt ttaatatcag aaatggaatc tgttccatta
     3841 ccatataatg caaagttaat atctaaacgt atttcaccgt gtgcaaagac atcttgctgt
     3901 gcaagtgcat ctgccacaat gttgattgtt ccttctatag aattttcaat agggacaaca
     3961 ccaatcgatg tgtcatcatc tgcaactgcc ttgatgactt caaataaatt tgactttggt
     4021 tgaaaagttg tttccttttc agaaaaatac tgacgacaag ccaaatatga aaatgtacct
     4081 ttagggccta aataatataa ttgcatatgc tacacctcta ctaacttaat gatggaaagg
     4141 gcactggtta gcatttgatt ctttcttttt atagaaaaag tttggatctt ttactgtatt
     4201 gtcatatcca tgatgataat ttgacgtcaa tgttggagat aatggcggcg ctagccaaga
     4261 ccattttccg gtaacttgac gaccttgttg tgcttcatta cgttcgaata gttcgaattg
     4321 ctttgcagcg gtcaaatgat cgacaattga tacgccttct tttttaaagg aatgatacac
     4381 agcatagttc aattcaacaa gtgctcgatc tttattaaat gaattatttt taagtgtatc
     4441 aaattcaaac gcatctgcaa ctttttctag taaattgtaa cggtaatcat caataaagtt
     4501 acgtacgcca atttcagtta ccatatacca accgttaaag ggtgcagttg gatatacaat
     4561 gccaccgatt tttaagtcca tattggaaat gatagggact gcataccatt ttaagttcaa
     4621 ttttcttaat tttggataat gattatgttc aataggtact tctttaatta atgaagtagg
     4681 atattcgtaa aatttaactg actcattagg taattggtaa atcagtggta acacgtcaaa
     4741 attagtacct tttcctttcc aacctaagtg atttgctaag cgtgtaactt ctttttcagc
     4801 aggatcacca caattgtcat agccagcata gcgaattaat tgattgttga aaattttagg
     4861 tccatccttt ggagcatata tagtaatata cggctttaat ttaccttcat ttgtagcctg
     4921 tgtaatatga taagtaattg atgataagaa cgatgcttcg tcagtaacat ctcttgcatc
     4981 aatgacattt aacgaatccc aaaataaacg accaatgcaa cgatttgaat tacgccaagc
     5041 cattttagca ccataaataa gttcttcttc tgtatgtgta tatgtcccag tttcttttat
     5101 ttctagttca atgtcatgta aacgtttatt gataatttgc gtttcataat gacactcttt
     5161 atacatgttt tctatgaaag cttgagcctc tttaaataac attaaca
//
LOCUS       DAMZLE010000152         5199 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMZLE010000152 DAMZLE010000000
VERSION     DAMZLE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.155"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            34..741
                     /gene="splA"
                     /locus_tag="POB92_002364"
     CDS             34..741
                     /gene="splA"
                     /locus_tag="POB92_002364"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDI6579829.1"
                     /translation="MNENVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            866..1588
                     /gene="splB"
                     /locus_tag="POB92_002365"
     CDS             866..1588
                     /gene="splB"
                     /locus_tag="POB92_002365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDI6579830.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGITLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1646..2365
                     /gene="splC"
                     /locus_tag="POB92_002366"
     CDS             1646..2365
                     /gene="splC"
                     /locus_tag="POB92_002366"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDI6579831.1"
                     /translation="MNKNIVIKSMAALAILTSATGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPDGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQALNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYDGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2486..3205
                     /gene="splF"
                     /locus_tag="POB92_002367"
     CDS             2486..3205
                     /gene="splF"
                     /locus_tag="POB92_002367"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDI6579832.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            3270..3404
                     /locus_tag="POB92_002368"
     CDS             3270..3404
                     /locus_tag="POB92_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579833.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            3568..5124
                     /locus_tag="POB92_002369"
     CDS             3568..5124
                     /locus_tag="POB92_002369"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6579834.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
BASE COUNT         1984 a          793 c          973 g         1449 t
ORIGIN      
        1 aactataaaa ataaatttgg aaggaggtaa aatatgaatg aaaatgtaat ggttaaaggt
       61 ttaactgctt taactatttt aacatctctt ggttttgctg aaaatatttc taatcagcct
      121 cattcaattg ccaaagcaga aaagaatgtc aaagaaatta ccgatgcaac taaggaacca
      181 tacaattcag tggtagcatt tgtgggtggt actggtgtag ttgttggtaa aaatacaatc
      241 gtaactaaca aacatatcgc taaaagtaat gatattttta aaaatagagt atcagcacat
      301 cattcgagta aaggtaaagg cggaggaaac tacgacgtta aagacattgt agaatatccc
      361 ggaaaagaag accttgcgat agttcatgtt catgaaacaa gtacagaagg tttgaatttt
      421 aataagaacg ttagttatac aaaatttgca gacggagcaa aagtgaaaga tagaatttct
      481 gttattggtt atccaaaggg tgcacaaaca aaatataaaa tgtttgaatc gacaggaacg
      541 attaaccata tcagtggaac gtttatggaa tttgatgcgt atgcacaacc aggtaattca
      601 ggatctcctg tattgaattc taaacatgaa ctgattggta ttttatatgc aggtagtgga
      661 aaagatgaat ctgaaaagaa tttcggtgtt tatttcacac cacaattaaa agaatttatt
      721 caaaataata ttgaaaaata agtatcatca attcattcgt gaagttgatt ttttaaagag
      781 tgattaagaa aacggttaca taattaacta aatatactga attatgtatc tagatatcaa
      841 aataattaaa aaagaggaac ttaaaatgaa caaaaacgta gtcatcaaga gtttagcagc
      901 attaacaatt ttaacatctg taacaggtat tggaataaca ttggttgagg aagtacaaca
      961 aactgccaaa gcagaaaata atgtcacaaa agttaaagat acaaatattt ttccatatac
     1021 tggtgtagtt gcttttaaaa gtgcaactgg atttgtagtt ggaaagaata ctattttaac
     1081 aaataaacat gtgtcgaaaa attacaaagt gggcgataga attactgcac atccaaatag
     1141 tgataaaggt aatggtggta tttattcgat taaaaagatt attaattatc caggtaaaga
     1201 agatgtatca gtcattcaag ttgaagagcg tgcaatagaa cgtggaccaa aaggctttaa
     1261 ttttaatgat aatgtaacgc cattcaaata tgcggcaggg gctaaagctg gtgagcgaat
     1321 taaagtgatt ggttatccac acccatacaa aaataaatat gttttatatg agtcaactgg
     1381 ccctgtgatg tcagtagaag gtagcagtat tgtatattca gcgcatactg aaagcggaaa
     1441 ctctggatca cctgtattaa acagcaacaa cgaattagtt ggtattcatt ttgcttctga
     1501 tgtaaaaaat gatgataaca gaaatgcata tggcgtctac tttacaccag aaattaaaaa
     1561 attcattgca gaaaacatag ataaataaac aaattgactt taaacgagcg ttgcaacata
     1621 tctcgaattg taaaggagct tgaaaatgaa taaaaatata gtcattaaaa gcatggcagc
     1681 attagccatt ctaacctcag caactggaat aaatgctgca gtcgttgaag agacacaaca
     1741 aatagcaaat gcagagaaga atgttacgca agttaaagat acaaatattt ttccatataa
     1801 tggcgtcgtt tcatttaaag atgcgacagg ttttgtaatt ggaaaaaata caattatcac
     1861 caataaacat gtatcaaaag attataaagt tggcgataga attactgccc atccagacgg
     1921 tgacaaagga aatggtggta tatataaaat taaaagcatt tctgattatc cgggtgatga
     1981 agacatctct gtcatgaata ttgaagaaca agcagttgaa cgtggaccaa aaggctttaa
     2041 ttttaatgaa aatgtccaag cattaaattt tgcgaaagat gctaaagttg atgacaaaat
     2101 taaagttatt ggttacccat tacctgctca aaatagtttt aaacagtttg aatctacagg
     2161 aactataaaa agaatcaaag acaatatttt aaattttgat gcatacattg aacccgggaa
     2221 ttcaggatca ccagttctaa attctaacaa tgaggtcata ggtgtggtgt atgacggtat
     2281 tggaaaaatt ggttctgaat ataatggtgc cgtatacttt acgcctcaaa tcaaagattt
     2341 tattcaaaag cacattgaac aataaacaaa tttaaatata caccatgagc atgtgttcaa
     2401 taattttaat gaaaaacatc ggtcgaatat aacataaaaa aacgtctata tcaaaagcat
     2461 catgaataaa cagaggagca caaaaatgaa taaaaatata atcatcaaaa gtattgcggc
     2521 attgacgatt ttaacatcaa taactggtgt cggcacaaca gtggttgatg gtattcaaca
     2581 aacagccaaa gccgaaaata ctgttaaaca aattacaaat acaaatgttg caccatacag
     2641 tggtgttaca tggatgggcg ctggaacagg atttgtagtt ggaaatcata caatcattac
     2701 caataaacat gttacctatc acatgaaagt cggtgatgaa atcaaagcac atcctaatgg
     2761 tttttataat aacggtggtg gactttataa agttactaag attgtagatt atcctggtaa
     2821 agaagatatt gcggttgtac aagttgaaga aaaatcaaca caaccaaaag gtagaaaatt
     2881 caaagatttc actagtaaat ttaatatagc atcagaagct aaagaaaatg aacctatatc
     2941 agtcattggt tatccaaatc ctaatggaaa taaactacaa atgtatgaat caactggtaa
     3001 agtattatca gtgaatggga atatagtgtc atcggatgca attattcagc ctggtagctc
     3061 tggttcacct atattaaata gtaaacacga agctattggt gtaatctatg ccggtaataa
     3121 gccatcaggt gaaagcacaa gaggatttgc tgtttatttc tctcctgaaa ttaagaaatt
     3181 cattgcagat aatttagata aataattaaa acttagacat tcacccaatc ctgacaaaat
     3241 atactataac taacatttat taatatatat tgcattattt aatatgcatc aaagccaatc
     3301 aacgattgat tttcaccaac tcaattgttg attggtttta tttatgtatg aatgaacaac
     3361 tttttgacat cattaagaat ataaatgatt ttgaaagcat ttgaaagcta caacatttct
     3421 ataaaatttt tcaataacaa ttgcgccact aaaactcaaa atttccacca ccaacatcca
     3481 aattatcaac atcgcaacat aaccaaatgt tataataaat ctattacaca aagagataaa
     3541 ttacttatgc aaaggcggag gaatcacatg tctattactg aaaaacaacg tcagcaacaa
     3601 gctgaattac ataaaaaatt atggtcgatt gcgaatgatt taagagggaa catggatgcg
     3661 agtgaattcc gtaattacat tttaggcttg attttctatc gcttcttatc tgaaaaagcc
     3721 gaacaagaat atgcagatgc cttgtcaggt gaagacatca cgtatcaaga agcatgggca
     3781 gatgaagaat atcgtgaaga cttaaaagca gaattaattg atcaagtcgg ttacttcatt
     3841 gagccacaag atttattcag tgcgatgatt cgtgaaattg aaacgcaaga tttcgatata
     3901 gaacatctag cgacggcgat tcgcaaagtt gaaacatcta cattaggtga agaaagtgaa
     3961 aatgacttta tcgggctgtt cagcgatatg gatttgagtt caacgcgact aggtaacaat
     4021 gtcaaagaac gtactgcttt aatctctaaa gtcatggtta atcttgacga cttaccattc
     4081 gttcacagtg acatggaaat tgatatgtta ggtgatgcat atgaattcct aatcgggcgc
     4141 tttgcggcga cagcgggtaa aaaagcaggc gagttctata caccacaaca agtatctaag
     4201 atactggcga agattgtcac agacggtaaa gataaattac gtcacgtgta cgacccaaca
     4261 tgtggttccg gttcattgtt gttacgtgtt ggtaaagaaa cgcaagtgta tcgttatttc
     4321 ggacaagaac gtaacaatac tacatacaac ttagcacgca tgaatatgtt attacatgat
     4381 gtgcgttatg agaacttcga gatccgtaat gatgacacat tggaaaatcc agccttttta
     4441 ggcaatacat ttgatgcggt tattgcgaac ccaccataca gtgcgaaatg gacagcagat
     4501 tcaaaatttg aaaatgatga acgattcagt ggttacggca agcttgcgcc aaaatccaaa
     4561 gcagactttg cctttattca acacatggta cattacctag acgatgaagg taccatggca
     4621 gtcgtactcc cacatggtgt attattccgt ggtgccgcag aaggtgtgat tcgtcgctat
     4681 ttaatagaag aaaagaacta cttagaagcc gtgattgggt taccagccaa tattttctat
     4741 gggacaagta ttccaacatg tattttagta tttaaaaaat gtcgccaaca agacgacaac
     4801 gtactattta tcgatgcatc caatgatttt gaaaaaggaa aaaatcaaaa ccatttaagc
     4861 gatgcccaag tcgaacgtat tatagacaca tataagcgta aagcaacgat tgataaatat
     4921 agctacagcg cgacattaca agagatcgcc gataacgatt acaacttaaa cataccgcga
     4981 tatgttgata cattcgaaga agaagcacca attgatttag atcaagtcca acaagatttg
     5041 aaaaatatcg acaaagaaat cgcagaaatt gaacaagaaa tcaatgcata cctgaaagaa
     5101 cttggggtgt tgaaagatga gtaatacaca aacgaaaaat gtgccagagt tgagattccc
     5161 aggatttgag ggcgaatggg aagagaagaa gttagggaa
//
LOCUS       DAMZLE010000153         5185 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMZLE010000153 DAMZLE010000000
VERSION     DAMZLE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.156"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            272..586
                     /locus_tag="POB92_002370"
     CDS             272..586
                     /locus_tag="POB92_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6579835.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFFQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            586..1878
                     /gene="arsB"
                     /locus_tag="POB92_002371"
     CDS             586..1878
                     /gene="arsB"
                     /locus_tag="POB92_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDI6579836.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHIGFIRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNALNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            complement(1965..2819)
                     /locus_tag="POB92_002372"
     CDS             complement(1965..2819)
                     /locus_tag="POB92_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDI6579837.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3095..3319)
                     /locus_tag="POB92_002373"
     CDS             complement(3095..3319)
                     /locus_tag="POB92_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579838.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            3518..3988
                     /gene="sigS"
                     /locus_tag="POB92_002374"
     CDS             3518..3988
                     /gene="sigS"
                     /locus_tag="POB92_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HDI6579839.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            4101..4544
                     /locus_tag="POB92_002375"
     CDS             4101..4544
                     /locus_tag="POB92_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDI6579840.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(4531..4974)
                     /locus_tag="POB92_002376"
     CDS             complement(4531..4974)
                     /locus_tag="POB92_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579841.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT         1742 a          842 c          672 g         1929 t
ORIGIN      
        1 tcgtaaaaac aaagcttctt aatcaatacc cttgattaca aaataaattc aactaaggtg
       61 attggccata cactcaagat atggctaatc acctttttca atgcaaaaca aagagtatat
      121 tttgcaaaat caatatctat aatttatctt taataccctt ttaatactaa aaccaaattt
      181 aaattacata gtcatatcgt gtaatatacc aaaggaacat agacattcat ctatatagat
      241 gataatctat ttaacgtcta ggaggtatgt tatgacgtat aaagaactag caacattttt
      301 aaaagtttta tcagattcaa gcagattaga aatactagat ttactttctt gtggagagtt
      361 atgcgcttgt gatttgttag cacattttca attctttcaa cctacactta gctatcatat
      421 gaaagcatta gtaaaaacca acttagttac gacacgaaaa atcggaaata aacatttata
      481 ccagcttaat cataatattt ttgagtccgt aattaataac ttgtcaaagg ttcatacctc
      541 taaccaacga tgtatttgtc ataaccttaa gactggtgaa tgctaatgat gacaacttta
      601 gcgacactca tttttctagt aactttatta tttgtattat ggcaacctaa aggcctagat
      661 attggcatta ccgcattaac tggtgccttt attgctgtta ttactggtgt tgtaagtttt
      721 tccgatgttt tcgaagtaac aggtattgtt tggaatgcta ctttgacttt tgtctcagtc
      781 attcttattt cattaatatt agataaagtt ggattattcg aatggtcagc tattcacatg
      841 cttcatgctt caaaaggcaa tggtttaaaa atgttcgttt atatcatatt attgggtgcc
      901 attgttgctg catttttcgc aaatgatggc gcagcgttaa tcttaacgcc tattgtatta
      961 gcgatggtta aaaatatagg ttttagtaag cgggccatat tcccttttat tattgcgagt
     1021 ggttttatag ctgacacaac ttctttacct ttgatcgtga gcaatctagt gaatattata
     1081 tctgctgatt attttcatat aggattcatt cgatatttta gtagaatgat tatacctaat
     1141 ttattctcac ttttagcaag tattatagta ttgtggttat attttagaaa ggcgatacct
     1201 aaaacgtttg atgataataa tataaagcat cctaaagatg ccattaatga tttaaagcta
     1261 tttaaaattt catggattgt gctagttata ttacttttcg gctatcttat cagtgaattt
     1321 actaaaattc cggtatcaat tttcactgga atcattgctt ttatttttct aatgttggct
     1381 cgtaaatcaa atgctttaaa tattaagcaa gtcattaagg gcgcaccttg gaatatagta
     1441 ttattttcaa ttggtatgta tatcgtcgta ttcggcttaa gaaatgctgg cattacttta
     1501 atattggcta aaatattaga atatatttcc aattacggtc tatttagcac tattttggga
     1561 atgggcttca tttcagcgtt tttatcatca ataatgaata atatgcctac agttttaata
     1621 gatgcgattg ctattggtca atcaaatgtc catggcatgt taaaagaagg cctaatttat
     1681 gcgaatgtta tcggttctga tttaggtcca aaaattacac cgataggctc tctagctaca
     1741 ttactgtggt tacacgtctt aacacaaaaa gatgttaaga tttcttgggg cacatacttt
     1801 aaaaccggta tcatcattac aattccagta ctatttataa ccctcatagg gttgtatcta
     1861 acacttatca tattttaatt aataaaaaga cgctttctat ttattgcgat agaaagcgtc
     1921 tttttatact tgagcaagtt atatcataac ttaactctat caaattactt attcaaatgt
     1981 ttactgtcat ctttatacac aaagtatttg aagtattttc cttcagtctt catgttctta
     2041 taaaagtcag cgataattgt tgcattactt tctgcccact taatatcagt agcatattga
     2101 tgttctcctg gattttttgg attccatctc atactataca atgtattttg atctgtgctt
     2161 gataagaagt gcttatgaat gaaatcagca ccgcctgaaa tagctttttc aggtgtatcc
     2221 caaccatgct ttttagcata ttctgcacct gttttaattg ggtctttatc aagggctcct
     2281 actccataga aattgtagta ttttttgcca tcaatttcga ctccattagc taattcactt
     2341 ttaactgcgc cagtttctaa taatgcatgt gaaattaaat aaacttcgtt aacgtgctta
     2401 tctttagcag cttttaagaa atcatccgta tgtttcaata acgttggtct atctactaac
     2461 atacgtttaa ttctattttt atcaatccct tgatactttg ataaatctaa aaattgatat
     2521 ttttgctttt cattatcgat aaaagtaccg ctatccattg cacttttaat ttcagttgca
     2581 gatgcatctc tccatgcatc attcttttta tttgatacct gttgactcgt ataattattt
     2641 atttgtttct ttgctgcatc gtttaatgta acatttaact tttcaatctt aatgtcggat
     2701 ttaacatgtt tgaaaaatat ctgatcggat atcattgaga aaaataaaaa tgagacaaca
     2761 gcaaatatga caacaagtcc tattattcca aaaatagaac ctttcttgtg tttattcata
     2821 tccacacctc ttaggtcatt gttgttatac attcagtttg attacgataa taaagtttaa
     2881 cattattaaa accgtttaac aattttatct atgcattgtt acatattcat aaataaacag
     2941 taatgtaaat gaaacgtttt aaaccattta tcaacattac tttacgttga tttacgaatc
     3001 ataaatgttt tttctttttg tataaccttt gaaaaccttt atatcaataa tcttattatt
     3061 cacgtattaa aaaacaaaaa attctcttgt tcgtttactt agtttctttt ttataatcca
     3121 tcataataaa acttgctgca tttgatctat ttttataata gtactgattt aaatcaatcg
     3181 taccttcaat ttgaccatca cgatacatca tttcattagt gaagttattt atcatcacga
     3241 aatcagcgcg atctcttata acatctaaat cgtcgtttcg tgcaaaaaaa tgttctaaat
     3301 ttaaatgtgc gtaatccatt gttacctccc gattacaaat tttgttctat ctactattta
     3361 gcagagtaat aatacatcat atcacattaa attaattact tatgtcttat atttaacact
     3421 taatttagcg attacgcaaa tgaatgattt ttctaaatat tttattctaa ctttatccgg
     3481 aaatatttgt tatacgtata acttggagaa tgactacttg aaatttaatg acgtatacaa
     3541 caaacaccac aaaatcatac accatctttt aaaaaaatat aatattagct ataattatga
     3601 tgagtattat caactactct tgataaaaat gtggcaattg agtcagatat ataaaccctc
     3661 aagcaagcaa tctttatcct cttttttatt cactcgatta aatttttacc ttatcgattt
     3721 attcagacaa caaaatcaat taaaagatgt cattttatgt gagaataatt caccaacatt
     3781 aactgaacaa ccaacatact ttaatgaaca tgaccttcgt ttacaagata tcttcaagct
     3841 tttaaatcaa agagaaagac tatggctcaa actatacctt gaaggataca agcaatttga
     3901 aattgctgaa atcatgtcat tatcgctttc aacgattaaa ttaattaaga tgtccgttaa
     3961 gcgtaaatgc caacataatt ttaattagtt tattgtgaaa tttcggtcga tgtgttaatg
     4021 atgttgaaac accctaacat cttcatataa atttatttta accattaaat taacaactta
     4081 ttatacgagg agtactatat ttgcaagaca attctactaa atatctactt tatatccaaa
     4141 ctgctacttc aaaccatctc gaaacaaatt gtgtcttttt acattgcgat tacattctta
     4201 aagttccaat taacaaactc gtttcatatt atgcgaaatt gcatttatca tcacaaagtg
     4261 tgctaattga gactgcaaaa aacatactaa atattaataa actggttcct atttacatca
     4321 acccaaaaac tatacttttt ccgttaaaac ataaacgtgc accgatacaa atttatatca
     4381 atgcacatta tattgttggt atgactgcca tagagaattc aacattaata cattttcaag
     4441 aaggcattca gctagaagtt gatgagccat tttctctagt ttcaaaaaaa tgtcatgaaa
     4501 gtttagcttt gaagcacttc attgaaaata ctatttcgaa ttaaatttaa tttctaaaag
     4561 tttcttagta aagtatgatg tcacaatttt tataaaatat atgctaacaa tcattaataa
     4621 aatcgctcca ataattgtta aaataacaac agttgttgat aaatctacct taggactaac
     4681 gaaaaattgt acgccagata gacttgtaat tagcccaaat aaaaacaagc ctaataaagc
     4741 aacataagca aacatcatca ctactaatgt aacaaacata ctataacttt ttgtttcttt
     4801 atttcgaaat acattttgtc ttaagtaagc attagcaaag gtaacaggct tatttaagtt
     4861 aacgctgtca tcatcagata tagaagacaa tcgttgatct aatgcttgtt tctccttctt
     4921 gttcaaaaac cataattgct ttttaacttt tttatcaaat gatgctttat tcataattaa
     4981 ggatttctct cctttataat attgacgtaa ttttaaacat tagcgtcaat tgaaagtgta
     5041 ttttatatat tttacagaaa tatatgtatc aaatctacaa aaatccatat tacaatttca
     5101 tttatggtga tttgacgcta aaatacagtc tgctaatttt ccaaactaat gctttttagg
     5161 taggtacaat gataaatttt aatcg
//
LOCUS       DAMZLE010000154         5157 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMZLE010000154 DAMZLE010000000
VERSION     DAMZLE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.157"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(1287..1670)
                     /locus_tag="POB92_002377"
     CDS             complement(1287..1670)
                     /locus_tag="POB92_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDI6579842.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            1853..3295
                     /locus_tag="POB92_002378"
     CDS             1853..3295
                     /locus_tag="POB92_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDI6579843.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLTEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            complement(3380..4324)
                     /locus_tag="POB92_002379"
     CDS             complement(3380..4324)
                     /locus_tag="POB92_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDI6579844.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(4370..4993)
                     /locus_tag="POB92_002380"
     CDS             complement(4370..4993)
                     /locus_tag="POB92_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6579845.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
BASE COUNT         1806 a          784 c          813 g         1754 t
ORIGIN      
        1 gatagtaagc aaaatttgga aattttcgat agagtctaat taaattattt ggtgcataat
       61 taatatagat ttcaaaccta tgtatttcaa aaaacaatac atagccattt tgaactaact
      121 tgctaatctt ttattgatat tcaactatgt tataaaaaaa gcaaattgac caaaatggaa
      181 gtttgaatca aaaaacatta aatgtaccca tgacttaaat tttatcgcca ttatttaagt
      241 ttggacatac acttttgtca tcaaacgctg aaaattagta cgattgaaat cacttcttgt
      301 tataaaaatt cttagtagcc ctgttacaaa atattcgcta caaattattc aaatcaacaa
      361 caataaccat caactaagaa attttaaaca atctactaaa aaataaagcg tttaaaagtt
      421 ctgtttagaa aacatcaaga taagtataaa catctagaaa agtgaacatc taagaaaatc
      481 catctagtta ttgccgtaac aattagaata atcatttgaa aaagtgcatt agacaacgaa
      541 attcaccatc aagataatga agcaacaaga aacagcacac atcaatgaat taacacctta
      601 gattcatcat gagaaaatgc atggcacaac tagatttacc atcaagataa ttcttaagca
      661 gcgaagaatt aacatctaga aatgcataaa catcttagat tttacatcaa gagatgcgat
      721 aactataaac aactagaaac gtcaccaaga tagtgcaaca acaaagaatc agcatcttga
      781 aatcgcacta acatctaaga atcaacatca agtattatat taacatcata gataacttca
      841 tcaagacagg catccacatc taagaaactc atctagaata gctaacacct aacatcaaag
      901 aatattcatc aagaacagta tctaacagaa ctttttgact atatatgaat taattatgtt
      961 actagctaaa tcatctatta ttaactttca cactcaattt ctacatctaa tatcaattta
     1021 ttaacaatag aattagcagc aacaagatat tacttaaaat tactcccccg aatttaaaat
     1081 ataaacaatg taataattaa gggtataaat tgatttatgc tgtaactaat taaatgtgta
     1141 tggtatcttc taacatcatg aaacgcattt taatatagat atgtacattt aaaccttgag
     1201 agatagatta tagtaaactt tagtacaaag tatgaattgc tgaactccaa tgactactat
     1261 ttgtccgtta caactaaagt ttcaatctat ctctttcttt ttgtgtttaa aacacgactt
     1321 tgtagtaaat attgaacatc atatttatta gcaagttctg ctaaaaaatc taataaagtt
     1381 tgggtgtcga ttttaccatt aataaaatca gctttaagta gttttgattg ttgtctttct
     1441 tcttcattcg cacatttttt aaaatatccc cacatatgat caaaagcatt acatacactt
     1501 cctatactcg gttcaattaa caaagcatca tcaatcattt gttgaacttg atgaagtgat
     1561 aaatccgttt ttaaagcatt ccttatcatt tggtaagact gttgactgtg taacaataca
     1621 tgatattttt cctcacgcca aagctgttcg atatagccac gttctttcat aaaattacct
     1681 ccgataatta atgttaatat acatgcatgt agtatagcaa taaaaatgaa tgcgctatca
     1741 aaaatggtgt aggtgtttat ataaaatgat aatagagtgt tgcaaatatt tttggaaccg
     1801 gttacaataa agtggaataa atggaaccgg ttctaattag gaggctaaat atatgaatta
     1861 taagcaatcc gcagaagaaa ttttgaacgc gataggcgga gaagagaatt tagatgcaat
     1921 ggcacattgt gcaacgagac tacgattagt tttaaatgat gaaagtttag taaatgaaga
     1981 ggcgctaaac aatatggatg tagttaaagg gacgttttct actgggggac aataccaaat
     2041 tattattggg tctggtacag tcaataaagt atttagtgaa ctggaaaaat taactggaaa
     2101 agaagcatca accacttcgg aagtcaaagc acaatctgct aaaaatatga atccgttaca
     2161 gcgatttgta aaaatgcttt cagatatctt tgttccgatt ataccagcca tcgttgctgg
     2221 tggtttatta atggggttaa ataacatttt gactgcgaaa gatttattct tttcaggtaa
     2281 atcattgata gatgtatata gtcaatttgc tggattaact gaaatgataa atgtttttgc
     2341 gaatgcacca tttacattat taccaatttt aattggattt agtgcagcaa aacgctttgg
     2401 tggcaatcca tttttaggtg ctgcattagg tatgatacta gttcatccat cgctaatgag
     2461 cgcatacgat ttcccaaaag cagttgaagc aggtaaggct attccatatt gggatgtttt
     2521 tggtttgcat attaatcaag taggttatca aggacaagtg ttacctatgc ttgtagcagc
     2581 ttatatctta gcctcaattg aaaaagggtt acgcaaagtt attccaacgg tgttagataa
     2641 tttgttaaca ccattgttat ctatttttat aacagcattt ctaacatttt catttgtagg
     2701 tccaatcact cgacaattag gttactggtt atcagatggt ttaacatggc tttatgaatt
     2761 tggtggtgca attggtggat taatattcgg attattgtat gctccgattg ttattacagg
     2821 tatgcatcat agctttatag ctgtagaaac gacattaatt gcagatgcca ctaaaacggg
     2881 tggatcattt atattcccga ttgcgacaat gtctaatgtt gcacaaggtg gtgcagcaat
     2941 tgcagcgttc tttattatta aacaaaataa gaagttaaaa ggtgtggcat ctgccgcagg
     3001 tatttcagca ttacttggta ttacagaacc ggctatgttt ggtgttaact taaaactaag
     3061 atatccattt attggcgcta tcgttggatc aggtattggt tcagcatata ttgctttctt
     3121 caaggttaaa gcaatcgcat taggaactgc tggattgcca ggatttattt caatcaatcc
     3181 agtacatgca ggatggttac actactttgt tggtatgaca atatcattca tcattgctat
     3241 aacagttact ttaattttat ctaaaagaaa agcaaataaa gaagttgtag aataaaaaag
     3301 aggtatgaca gtaatcaaag tatcagtcat tgcaaatgca ataggatgaa actttaagct
     3361 gtcatacctt ttaatttagt tataatttct gtttacgcca taagaaaata ctaacaatta
     3421 cgacaagaat taatgaaata cctaccacaa taatccaaga tatataacta tggtcatcaa
     3481 ttggcaacgg cacattcata ccgaagaaac taaatacgag tgttggtaac gttaatagta
     3541 ccgtgaaaag tgttaatgtc ttcataatcg tattcatatc attagataat aaggaagcgt
     3601 atgatgttgt gatactttct aagatacgtt gatgtaattc tgttgtttcg atggcttggt
     3661 tattttcaat gattaaatct tctagtaatt cttcatcttc ttcaaaacgt ttaattgcag
     3721 gtaaacggaa taacttttta ataattgtat cgttaccttt taaggcagct aaaaagtata
     3781 ctaaactttt ttcaacttcc attaatttga aaagttgttt gttagtaatg ttatttttta
     3841 gttctttctc aatacgaatt ctacttttat taagtaatcg taagttacga ttataatggt
     3901 cggcaatagt agttaaaatt tctaatgcaa atctgctgtg aaattttaaa ttaatatcac
     3961 gcttaggtaa attttctaaa aattcatttt cagcgtcaca aactgttacg ataataccgt
     4021 tgccaataat aatgcctaat ggaatcgtta caaaagatag aacgctacga ttagttgaat
     4081 tgacgatagg taaatcaata atgattaatg agtaaccagt atcttcgtca tattcaatac
     4141 gggaactttc ttctgagtcc aaagggtcac gtataaagtc ttcaggtata ttatattgtt
     4201 ccataagatt ttcaatttct tctcggtctg gttctactac attaatccat gacgctgtat
     4261 gatctaatgg tgtttttata atatgtttat ctatatcttc agaatgtctg aatgaagtaa
     4321 tcatgatata tagttccttt ctaaaggatt taggaaaagc gagtattact taagttagtg
     4381 aatcaaggtt tgatttatgc aaacggtttt ctgccattat tcttaatgga ccattaaaaa
     4441 taatattatg cacatgttta gctaattcat caactgaaat gggctgtttg tccattaccc
     4501 agtatttaat aattgaaatt gttgctccgg aaacgtaact gtaaaaatac atctctggaa
     4561 cgcctccgat ggaatgattg atactaatat aacgttgcat attattttta atatgttcgt
     4621 tgattttcaa ttctaattga ctcgtgcgtt ccaattgtaa aatagtatga taaaactcta
     4681 aattatcatg aatatgttgg aaaatatttt tgagtatatt atttacaaaa gtattcgcaa
     4741 tgtcttcaat tgaagaaccg cgtaacaaat caaattgagt atatgtcgtt agttctgaaa
     4801 tatactcatc ttccatatct gcgagtaaca tatatttatc ctcgtaatgt aagtaaaatg
     4861 tccctctatt gatatcagcg cgtgttgtta tgtcgcgaat ggtaatcttt tctaaatctt
     4921 tttcttgtag taatttagtg aacgcttgtt taatggagga ctttgtcttt ctaattcgcc
     4981 tatcttcttt caaaaataaa tcaacctcct cgtgttattg aacaatgcgg gtgtatttgt
     5041 ctattaataa acgtattgat gaacattgtg tattgtttat tcccacttac atattataat
     5101 agacaacatg cgaaatattc aacacattgt ctaataagga ggtaattaat aaaatga
//
LOCUS       DAMZLE010000155         5141 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMZLE010000155 DAMZLE010000000
VERSION     DAMZLE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.158"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            253..1710
                     /locus_tag="POB92_002381"
     CDS             253..1710
                     /locus_tag="POB92_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6579846.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1703..2425
                     /locus_tag="POB92_002382"
     CDS             1703..2425
                     /locus_tag="POB92_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6579847.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2576..3703
                     /gene="queG"
                     /locus_tag="POB92_002383"
     CDS             2576..3703
                     /gene="queG"
                     /locus_tag="POB92_002383"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI6579848.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLASFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILGLAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3708..4178
                     /gene="trmL"
                     /locus_tag="POB92_002384"
     CDS             3708..4178
                     /gene="trmL"
                     /locus_tag="POB92_002384"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI6579849.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4337..4936
                     /locus_tag="POB92_002385"
     CDS             4337..4936
                     /locus_tag="POB92_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDI6579850.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4961..5113
                     /locus_tag="POB92_002386"
     CDS             4961..5113
                     /locus_tag="POB92_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579851.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         1916 a          658 c          987 g         1580 t
ORIGIN      
        1 agcttgatga caagaataag ttttaatata tgataaatat aggcgttact gtcaataaga
       61 tattgatagt agcgcttttt tgattgattt taaaatgtga gatttgttca cgtataatag
      121 cggatttttt atgtacagtc ttttaaaaag aagattttct ttatattaat aaaaatacat
      181 ttataaaaca ctattgcatc aaaatgtatt aatatgtata attatgaata ttaattaggg
      241 agggctgtat aaatgaagtg tttaattagg tttatattag tattagggtt gttaattagc
      301 agtgctatgg tgtatatcaa tcccacagca catgctgaac aagatcaaac atgggagaag
      361 attaaagaac gcggtgaact tagagtgggt ctttctgcag attatgcacc aatggaattt
      421 gagcatacag ttaatggtaa gactgagtat gcaggtgtag atattgattt agctaaaaaa
      481 attgcgaaag ataataattt aaaattaaaa atcgtcaata tgtcatttga tagtttgtta
      541 ggagctctta aaactggaaa aattgatatt attatttccg gaatgacttc aacgcctgaa
      601 cgtaagaagc aagttgattt ttcagattca tatatgatga ctaaaaatat catgcttgta
      661 aagaaagata aagttaatga atataaagat atcaaagact ttaataataa aaaagtaggg
      721 gcacaaaagg gaactgaaca agaaaaaatc gctcaaaccg aaattgaaaa tgcatctatt
      781 acttcattaa gccgtttacc agacgttata ttagcactta aaagtggaaa ggttgaaggt
      841 gctgtagttg aaaaacctgt agcagaagca tatttaaaac aaaatcctaa attaggaatt
      901 tccaatgtga aatttaatga agaagaaaaa gatacagtga tagcagtgcc aaaagattca
      961 ccaaaattat tgtcacaaat taataaaacg attaaggagg ttaaagataa aggattaatc
     1021 gataaatata tgactaatgc tgcaaatgcg atgaatgatg acagtggttt tatttctaag
     1081 tatggtagtt ttttcttgaa aggaattaag attacaatat taatttcact tatcggtgtt
     1141 gcattaggtt ctattttagg tgcattcgtt gcgttaatga aattaagtaa aataaaaatt
     1201 atttcatgga ttgcttctat ctatatcgaa atattaagag gaacaccaat gttagttcaa
     1261 gtatttatcg tattctttgg tataactgct gcattaggac tagacatttc agctcttgtt
     1321 tgtggaacaa ttgcattagt tattaattcc tcagcttaca ttgctgaaat tattcgtgca
     1381 ggtataaatg ctgttgataa aggccaaatg gaagccgcac gtagtctagg tttgaattat
     1441 agacaaacga tgaaaagtgt aattatgcca caagcaatta aaaatatttt accagcttta
     1501 ggtaatgaat ttgtcacttt aattaaagaa tcatctattg tgtcaacaat tggagttggc
     1561 gaaattatgt ttaatgcaca agtggttcaa ggtatttcat ttgacccatt cacaccatta
     1621 atagtggcag cagcattata ctttgtctta acatttgtac ttacacgcat tatgaacatg
     1681 attgaaggga gattgaatgc cagtgattaa aataaacaat cttaataaag tttttggaga
     1741 taatgaagtt ttaaaagata tcaatcttga aatcaatcaa ggggaagtag tagcaataat
     1801 aggtccatct ggtagtggta aaagtacatt gttaagatgt atgaatttat tagaagtacc
     1861 cactaaaggt caagtgattt ttgaaggcaa tgacttaacg gaaaaaggga cacaagtaga
     1921 taaactacgt caaaaaatgg gtatggtatt tcaaaacttc aacctatttc cacataaaaa
     1981 agttgtcgat aatattattt tagctcctaa attattaaag aaagataata acgatgaatt
     2041 acataaggaa gcattgtcgt tattagataa agtgggatta aaagaaaaag cagatgtata
     2101 tccgaatcaa ttatcaggtg gtcaaaagca aagggtagca attgcaagag ctttagcaat
     2161 gcatccagat gttattttat tcgatgaacc aacttcagca ttagatcctg aggtagttgg
     2221 tgatgtatta aaagtaatga aagacctagc caaagaaggt atgaccatgg tggttgtgac
     2281 acatgaaatg ggatttgcca aagatgtaag tgacaaagtc atatttatgg cagatggcgt
     2341 tgtcgtagag tcaggcacac cagtcgaaat atttgaacaa ccgcaacatg aaagaacaca
     2401 aaatttctta gcaagagtat tataacaacc taacgaagct tgaatatatg atacgcacca
     2461 caaagttata tcatatattc aagcttttta ttattaaaat tatgtgaaat gaaacctcaa
     2521 tatgaataat ggttgtgtta aaataacata gtattattta atggggggca tacgattgga
     2581 tacaaagcag ttaaagcaag atatcattga ctatgcatat acaattggta tcgacagtat
     2641 tggatttaca actgccgatc cctttgatga attgaagcaa aagctagaag catatcatgc
     2701 aaatggttat gcctcaggat ttgaagaatc tgatattgct ttacgaacgg agcctaaatt
     2761 atccttacca acagcaaggt caatcatagc aattgcagtt ggttatccta acaaactgaa
     2821 aggtgcacct aagagtgtta gaggtgatcg cagaggctta tttgctagag catcgtgggg
     2881 tcaagattat catacaatta tgcgtaaacg attagacatg ttagcttcat ttattgaatc
     2941 taaagttcca gatgttgaaa taaaatctat ggtagatacg ggtgtattat cagatagggc
     3001 agtagcagaa cgtgcaggtt taggatttgt tggtcgtaat ggctttgtca tcaatcctaa
     3061 actaggaaca tggacatacc tcggtgaaat gttagtcagt ataccttttg aacctgatga
     3121 tccattatta gatagctgtg gtgattgtac aatttgtgtt gatcgttgtc caacaagtgc
     3181 attagttgga aatggccaat taaatagtca gaaatgcatt agttttttaa cgcaaacaaa
     3241 aggctatatg cctgaccaat atcgttataa aattgggaat agattatatg gttgcgatac
     3301 gtgtcaacaa gtttgtccga aaaatagagg tattaatacc gaacaagatg acatcatttt
     3361 ggaaccagaa attttaaagc caagattagt acctttacta cgtatgtcta ataaagaatt
     3421 caaacaaaca tatggtcacc ttgcaggtgc ttggcgtggt aaaaaaccga tacaacgaaa
     3481 tgctatttta ggattggcgc attttaatga agtggatgca attccagaat tgaaaaaagt
     3541 tgcaacaaca gatgaaagac cgatgattcg agcaactgct tattgggcca ttggtcaaat
     3601 tcttggggaa gaagcaagag attttatcaa tgcaaattat gatcaagagg atgcagaagt
     3661 tcaaaatgaa atgataaaag gattagacac aaggagagaa tagtaatatg acaaatcata
     3721 tcgttttata tcaaccagaa atcccagcta atactggtaa cattgcacgt acgtgtgcag
     3781 gtacaaacac acatttacat ttaattaaac ctcttggatt tagaactgat gataaaatgt
     3841 taaaaagagc cggtttagat tattgggaat ttgtgaatat tacgtatcat gatagtattg
     3901 aagcattttt tgaagccaca aatggcgagt attacttatt aacaaaattt ggtaaaaaga
     3961 cttatagtga ttttgatttt tcaaatcatg acaaagattt ttacttcatt tttggaaaag
     4021 agacgacagg attaccagac tgggtgaaag agaaatatca agacacagca ttaagaattc
     4081 ctatgagtga acatattcgt tcattaaatt tatcaaatac tgctgcgttg ttaatttatg
     4141 aagcgttacg tcaacaagat tttccgggat tgaattaaaa tttactcatt ttcattataa
     4201 aaatgatgct taagtgctat atttagagta taagcatgtt aaagttaaat ggtttgatat
     4261 attgaaagtt agggtataat aattattaat atcgccgttt ttatgtaaaa tgcaaactaa
     4321 gaggagtgta gaaatcatgg caatgaactt taaagtcttt gacaatagtc aacttgtagc
     4381 agaatatgct gctgatatta ttagaaagca atttaacaat aatcctacta caattgcagg
     4441 ttttcattta gatacagatc aagcgccagt tctagatgaa ttaaagaaaa atgttgaaaa
     4501 acatgctgtt gattttagcc aaataaatat tttagattat gacgataaaa aatcatattt
     4561 cgaagcgtta ggtgtaccag caggtcaagt ttatccaatt gcttatgaaa aagatgcaat
     4621 cgaattaatc gctgataaga ttaaaactaa agaaaataaa gggaaattaa cattacaagt
     4681 tgtttctatc gatgagcaag gtaagttaaa tgttagtatt cgtcaaggac taatggaagc
     4741 aagagaaatt ttcttagtag tgacaggtgc taataaacga gatgtagttg aaaaattata
     4801 tcaagaaaat ggtaaaacaa gcttcgaacc agccgattta aaagcacata gaatggtaaa
     4861 tgttattctt gataaagaag cggctgcagg tttacctgaa gatgttaaag cttactttac
     4921 gtcacgcttt gcttaattaa aaatctgtga ggtattgatt atgtctaaag acaaagatcc
     4981 aaaattaaat tatcatgaag aagaaaacag tatggtaacg gattttgaag atttaaaaga
     5041 attaggtaaa gaaatggaac aaatctctga tcaaaatgat caagaaaaga attctgaaga
     5101 agacagtcag taaagtatta taattgtgta atattcagtt a
//
LOCUS       DAMZLE010000156         4962 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMZLE010000156 DAMZLE010000000
VERSION     DAMZLE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.159"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(115..444)
                     /locus_tag="POB92_002387"
     CDS             complement(115..444)
                     /locus_tag="POB92_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579852.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETLYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTQLSNAL
                     FAIKFAH"
     gene            493..1230
                     /gene="sdpB"
                     /locus_tag="POB92_002388"
     CDS             493..1230
                     /gene="sdpB"
                     /locus_tag="POB92_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDI6579853.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1321..1680)
                     /locus_tag="POB92_002389"
                     /pseudo
     CDS             complement(1321..1680)
                     /locus_tag="POB92_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1877..2569)
                     /locus_tag="POB92_002390"
     CDS             complement(1877..2569)
                     /locus_tag="POB92_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDI6579854.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2954..3751
                     /locus_tag="POB92_002391"
     CDS             2954..3751
                     /locus_tag="POB92_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDI6579855.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATYFPEHQLLAEEKSNAMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            3884..4831
                     /locus_tag="POB92_002392"
     CDS             3884..4831
                     /locus_tag="POB92_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI6579856.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT         1737 a          641 c          896 g         1688 t
ORIGIN      
        1 ataactcgat taatgcatct ttacaatttg aagaaacttt ttaattgtat aatttatgta
       61 ataaagtgaa ttttaataaa taacttgttt aaatgacatt ttatgaaaaa taggttaatg
      121 ggcaaattta attgcaaata atgcgttaga tagttgtgta tgataaaaat taagatattg
      181 aattttgtta ccatagtcga cttgtcggag gggacgttta ttaagtagtg ttataatttc
      241 ataataggaa acttgatttt cgtttattat atataatgtt tcaaagcctt tgaacttatt
      301 caattcatta gtaactattt ggttaaattt ataaatatgc gtttgaatgt tccaatctgt
      361 cattaaggta ttatatttaa tcgtatcaat gatttgaatc aaattagtaa tttgttgtct
      421 catatactta gtttcgttta tcatgtagaa gtacctccaa aaatcaatta ttaaacagga
      481 aaggatataa cagtggaaaa tgaaaaaaag aaatacacgt ttaaagatat agcatggaga
      541 gatttgctta taattccaat tataggtgtt atattttatg cactcacaat tgcagcttac
      601 ataggaccaa ttttgtttgg ttattatgaa atcgatagta atctgatatt tgtagcgact
      661 atcacacaag ctttagcata tacgattggg attggcgcat tttatttatt tcatttaaaa
      721 gtgatgccat caaggcttcg agctggtttt gcgtatatca aaaaatattg gcttagattg
      781 tcaataacgt atattattgc tctcatctta atttatacat atgaatatat gacacagttt
      841 ctacctaaac atttgcaata tagtgaaaca gccaatgaac ttgagctaaa taaaatgttt
      901 gaagtgcctg catttttacc tgtagcattc ttattaattg tgattgtagg tccaatagtt
      961 gaagaaatcg tatttagaca tattttaatt ggagaattag gtaagaagtt taattttata
     1021 gctatgagta ttgtttcggt gttcttattt gcatttattc atgtgactga tgcaaagtcg
     1081 ccgtttgagt ttggtccata tctcatacta tccatcatat tagttttcac ttatttaaaa
     1141 tctggaagaa acctaggttc aacaattgca ttacatatag caaataattt tgtttctttc
     1201 gttatatcag taataagtat ttatgcgtag ataaagaggt cgggacatta agtttttagg
     1261 caatgaaaaa agctgatttc tattaattat ttgatagaaa tcagcttttt tgatatgcat
     1321 tttattatga aactgaataa ataaagattc aattagcgca tcagtattag gattcactct
     1381 aaaaagatta atagttttat aagaaggtgt ttgatcttga gctaaccaca tcattcgaat
     1441 actgttatga agtaattttt ctattctacg gccagaaaat tcagattgag tatatgaata
     1501 tgaggtgatt tttaacatca tttttagatg ataggatgtt gcgccacgat gatgtctgaa
     1561 ttcatcgaat tcgctatcag ctatcttttt aacaatttca ttaacatatc gcgaaatatc
     1621 attttgagga attctaacag cagtttctat tggtagtgta agttgggcaa agtgttttat
     1681 ttttttaagt atttcaaagt aaaattacat gttaatacgt agtattaatg gcgagactcc
     1741 tgagggagca gtgccagtcg aagaccaagg ctgagacggc accctaggaa agcgaagcca
     1801 ttcaatacga agtattgtat aaatagagaa cagcagcaag atattttcta attgaaaatc
     1861 atcttactgc tgttttttag gaatcgatgg ggtttaaaaa ttgatgatag ttatcaatgg
     1921 atatgtctct ggtttgaatc gtcttaccga atgaatacga aatatctata atattttcaa
     1981 tttcaagttc ggcagttttg tcttgagcaa tgtcataaat gactgcatgc caatgtccat
     2041 gtttacacaa aaggccaata aatataacgt tttcagcttt aatattatca tcaaccctat
     2101 gatctactaa catcacattt cgttctgtac aataaattaa aatgtcggaa aacagcattg
     2161 ggagtgtcat atgttgtgtg tcatcaaatt taatataaaa gtccatgcgt ttaagcaatt
     2221 ttcttaagcg tgtttgtgga agacttaaac attgttttat aattgcgttg atttcgcttt
     2281 tatatggtaa tgtcgagtac gactgacttt cattaagcac taaaaataag gccgataatt
     2341 gtgtttcaga taagtttaat gcaattttag attgtgcttg ttgtatttgg taaccaccat
     2401 ttttcccata atgcgcataa attttaacac cttgattttc taaatcatca atatctctta
     2461 aaattgtgcg tttggatacg ttgcaatatt tagctaattc taaagcggtc atttttttat
     2521 tttgttgaat tgcagtaatt attaaatttt gcctttcagc tttattcatg acagccacct
     2581 cctaaaagat gatatattac acaaagtata acataagagt gacaatgagg acattttcaa
     2641 tatcttttaa aaaaaatcgt aaaaattttg tggaaaacgc ttacatttta aaaaagattg
     2701 tgctatgata ttacttgtaa cagggggagc gattaaacaa aggggtagag ctatataaca
     2761 gatagctttt aatcgttcaa gttacatcac aacatcttca atatttatta cttactttcc
     2821 tttctatttg tcggctagca cgtgactagc cgattttttt atgcataaaa tgaagaagtc
     2881 ttcagttgta ttgttgattc ttttaatata gggtaataat aaaatgataa tgatatgaga
     2941 agtggtggac gaaatgacag ataaaacatt acagcaaata gataagttaa tatgttcgtg
     3001 gttgaaacaa atagacaatg ttattccgca attaattatg gaaatgacta cagaaactaa
     3061 gcgtcatcga tttgatttag ttacaaatgt agataaacag attcaacaac aatttcaaca
     3121 atttttagca acatattttc cggaacacca actattagcg gaagaaaaaa gtaatgcaat
     3181 gattacgaat gaaattaatc atttatggat tatggatccc attgatggaa ctgccaattt
     3241 agtaaaacag caagaagatt attgtattat attggcgtat ttttatgaag gtaaaccaat
     3301 gttatcgtac gtatatgatt atccgcataa aaagctttat aaagcaatac gaggagaagg
     3361 tgctttttgt aatggaatta agatggaaga accaccatcg ttaaaattgg aagacgctat
     3421 tatatcgttt aatgcacaag tgatgaatct agatacggtg caagatttat ttgacgcctc
     3481 atttagttat cgtttagttg gtgcttgcgg attagattct atgcgagtgg caaaaggtca
     3541 atttggggca catattaata caaatcctaa accttgggac attgctgcac agtttttatt
     3601 tgcagaacta ttgaatctaa aaatgacgac tttagatggt aaggcaattg atcatttaaa
     3661 gggtgcacct tttattataa gtaataaagc atgtcatgaa acggtactta aaattttaaa
     3721 tgcaaatggt ggttatcaaa aatacagata gatattctct agagataaaa aatgataaaa
     3781 aatagttata cgtctacaga agtatagtaa aaaaattatt ttactatata ataaatagag
     3841 aaaacagaca aagtatgtaa ggaatagtaa aggagtggtg ggattgaaac gttcgtctaa
     3901 aagtaaaatg agcctaccga aaaaaatatt tttatgggta ttcggtattt tagtaatctt
     3961 agcgattgta gctgttgtgt atgtagctgc taaaattttt attactggta ataagataca
     4021 caatccgtta gatcgcaatc attcagaatt aagagataaa aaagtgagtt taaacgatgg
     4081 tgatccattc acaatcgctt tatttggtgt cgattcagat gctgatcgta agaaaaaggg
     4141 tggcggagag cgtagtgata gtattatgat tttatctatc aaccctaaaa cgaagaaaac
     4201 tgaaatagta agtataccgc gtgatacaag agcggaaatt gttggccgtg gtactactga
     4261 aaaaattgca catgcctatg catatggtgg accaaatatg gctgttaaat cacttgaaaa
     4321 attaatgaat gtgccaattg atcattatgc gacaatcgat atggatggtt tgcataatat
     4381 gatagatagc atcggcggtg ttgatgtagt aagtaatgat acattcactg ttgatggtgt
     4441 tcgttttaca aaaggacagc agacacatgt aaatggtgat caagcattga agtttattag
     4501 aagtcgtaaa gaagaaggtg ctggtggtga ctttggtcga caacaacgtc agcaaattgt
     4561 acttgaagcg atggctaata agattgcaag tccatcatcg attacacatt ttaatagtct
     4621 aatgaatgaa attcaaaata atgttaaaac agatttaaca ttaggtgatt tgaatacgat
     4681 tagaagcaat tataaagatg ctaatgacac gattaataaa catcaattga gtggtcaagg
     4741 cggcattcaa agtgacggat tgtactactt cattcctagt gagcagtcaa aagctgaaag
     4801 tactaagtta ttaaaagata atctagagta gtaaactaag ggaaaataag ttatcaaaat
     4861 tgtacacaaa ttaattagat agaatgatct gtaaatagaa taacgaatag aactaatagc
     4921 ccgtaaatag aaggtgataa cactttgtat ttatgggcta tt
//
LOCUS       DAMZLE010000157         4934 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMZLE010000157 DAMZLE010000000
VERSION     DAMZLE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.160"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            197..2011
                     /locus_tag="POB92_002393"
     CDS             197..2011
                     /locus_tag="POB92_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6579857.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            2214..4823
                     /gene="clpB"
                     /locus_tag="POB92_002394"
     CDS             2214..4823
                     /gene="clpB"
                     /locus_tag="POB92_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDI6579858.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQFLDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT         1833 a          633 c          926 g         1542 t
ORIGIN      
        1 gttgcattac aaattggatt tcgaagtaag aatcatcatt ggtaattttt ttaatttaac
       61 agtattttag tcaatatgat tttgatgatg tcgtaattca ataatataca tatgcatttc
      121 aatattcttt taaacagcat aactaaattt aattaagcat ctaagaagaa cagttttaaa
      181 agggaagaat gcaaaaatga acaaaacaaa gggttttaca aagtataaga aaatgagata
      241 tatgccaggg ctcgatggtt tgagggcaat cgctgttcta ggaattatta tttaccactt
      301 aaacaagcaa tggttgacag gtggcttttt aggtgtggat acattttttg tgatctctgg
      361 ttatttaatt acaagcttat tactcaaaga gtatgatgac acaggtatca ttaaattgaa
      421 aagcttttgg atacgtcgtt taaaacgttt attaccagca gtcatagttt tattaatggt
      481 tgtagggaca gcaaccttat tattaaaatc agataatatc attagggtta aacatgatat
      541 tattgctgcg atattttatg tatcaaactg gtggtatata gcaaaagatg ttaattattt
      601 tgagcaattt tcatttatgc cattaaagca tttatggtct ttagcaattg aagaacagtt
      661 ttacatattt ttcccagtta ttttggttac attattgtta acaattaaaa agcgatacaa
      721 aataggattt attttttggg gagtatcaat aatttcttta gggttaatga tgtttatcta
      781 cagtattaat ggggatcatt cacgagtgta ttttggtaca gatactagat tacagacatt
      841 gttactgggt gttattttag cttttttatg gccaccgttt aaattgaaaa atgatccacc
      901 taaagttgta aaatatgtta ttgatagcat aggtagttta tcatttatag tacttatatt
      961 attatttttc attattaatg atgagacgaa ttggatatat gatggtggtt tctatttaat
     1021 atccatatta acgttattta ttattgccag tgtcgttcat ccatctacat ggatagcgaa
     1081 gatattttca aatccagtgt tagtatttat cgggaaaagg tcttatagtt tatatttatg
     1141 gcattttgca gtaattagtt tcgtacatag ttactatgta gatggacaga tacctgtata
     1201 tgtgtacttt atagatataa gtttaacaat tatatttgca gagctatcat atcgctttat
     1261 agaaactcca tttagaaaag aaggtattaa agctttaaat tggcgacctt cttatatacc
     1321 acaatttata agaatggcaa ttgtagtaac cttgttaatt ccatttatgt tgattttagt
     1381 aggtgcattc aataaatatg gtaaagacat tattggagaa aaagcgaata gctttgatac
     1441 cactattgaa gataattatt taatgcggat agcaccaatt gataacattc acattgatgg
     1501 cttagtaagt gagaagaaaa aggaatcttc cgacgtatat aataatatta aacctctttt
     1561 aatcggtgat tcagtaatgg ttgatatcgg tgagtcattt aagtcatcag ttcctaagtc
     1621 tagaattgat ggaaaagtag ggcgtcaatt gtatcaaacc ttacctttag ttaaagcgaa
     1681 ttattcacaa tataaaaaat catctgatca agtcgtatta gaattaggta caaatggcga
     1741 ctttactgtc aaacagctcg acgatttact taatcaattt ggaaaagcca agatttattt
     1801 agttaataca cgtgttccaa gaatttatga ggcaaatgta aatcgattat tagctgacgc
     1861 ggcgaaacga aagtccaatg tcacattaat tgattggaat aagcgatcac aaggacatag
     1921 tgaatatttt gcaccagacg gtgtacattt agagtacaaa ggagtcctag ctttaaaaga
     1981 tgaaatatta aaagcactta aaaagaaata acatatgttt aataagtcta gttaatgtgt
     2041 aacgtaacat tagctagatt tttttattca aaaaaatatt tacaaatatt aggaaattta
     2101 agtgtaaaag agttgataaa tgattatatt gggactataa tataattaag gtcaaagaaa
     2161 gtcaaagtca aaagtgcaac ttgaccaact ctacaaatta gaggtgaaat tatatggata
     2221 taaataaaat gacatatgct gttcaaagtg ctttacaaca agcagttgaa ctgagtcagc
     2281 aacataaatt acaaaatata gaaattgagg caattttaag cgctgcctta aatgaaagtg
     2341 aaagcttata taaaagtatt ttagaacgag caaatattga ggtagatcaa ttaaacaaag
     2401 cttatgaaga caaactaaac acgtatgcat ctgtagaagg tgacaatata caatatggtc
     2461 aatatattag ccaacaagca aaccaattga taactaaggc tgaatcatac atgaaagaat
     2521 atgaagatga atatatttca atggagcata ttttacgttc ggcaatggac attgatcaaa
     2581 caacaaaaca ttatataaat aataaagtag aagttatcaa agaaattatt aaaaaagtaa
     2641 gagggggaaa tcacgtgaca tcacaaaatc cagaagttaa ttacgaagca ttagctaaat
     2701 atggccgcga cttagtagaa gaagttagac aaggtaaaat ggatcctgtt ataggaagag
     2761 atgaagaaat tcgaaatacg attcgtattt taagtcgtaa aactaaaaac aaccctgtgc
     2821 tcattggtga accaggtgtt ggtaaaactg caattgttga aggattagcg caacgtatag
     2881 ttaagaaaga tgtgccagaa tcattattag ataaaactgt ttttgagtta gatttaagcg
     2941 cattagtagc gggcgctaaa tatcgtggtg aatttgaaga gagattaaaa gcagtcctaa
     3001 aagaagttaa agagtctgat ggtagaatta tattatttat tgatgaaatc catatgcttg
     3061 taggtgctgg taaaacagat ggtgccatgg atgcaggcaa catgctaaaa ccaatgttag
     3121 cacgaggaga gttacattgt attggtgcaa caactttaaa tgaatatcga gaatatattg
     3181 aaaaagattc ggcattagag cgtcgtttcc aaaaagtagc agttagtgag cctgatgttg
     3241 aagatacaat ttcaatttta cgtggtttaa aagaacgata tgaagtgtat catggtgtgc
     3301 gtattcaaga tagagcctta gttgctgccg ctgaattgtc tgatcgttac atcactgatc
     3361 gttttttacc agataaagcg attgatttag ttgaccaagc atgtgcaaca attcgtacgg
     3421 aaatgggatc aaatccaact gaattggatc aagttaatag acgtgtcatg caattagaaa
     3481 ttgaagaaag cgcacttaaa aatgaatctg acaatgcgag caaacagaga ttacaagaac
     3541 tacaagaaga gcttgccaat gaaaaagaga aacaagcagc acttcaatct cgtgtagaat
     3601 cagaaaaaga aaaaatagca aatttacaag aaaaacgtgc gcaactagat gaaagtagac
     3661 aagcgttgga agatgcacaa acaaataaca atttagaaaa agctgctgaa ctacaatatg
     3721 gaacaattcc tcaattggaa aaagaactta gagaattaga ggataatttc caagatgagc
     3781 aaggtgaaga tacagatcga atgattcgtg aagttgtaac agacgaagaa attggcgata
     3841 ttgtcagcca atggacaggc ataccagttt caaaattagt tgaaacagaa cgtgaaaaat
     3901 tacttcactt aagtgacatc ttgcataaac gtgttgtagg tcaagataaa gcggttgacc
     3961 tggtttcaga tgcagtagtt agagcaagag caggtattaa agatccaaac agacctattg
     4021 gtagtttctt attcctaggt ccaactggag taggtaaaac tgaattagct aaatcattag
     4081 ctgcatcatt atttgattct gaaaaacata tgattcgtat tgatatgagt gaatatatgg
     4141 aaaaacatgc agtatcaaga ttgatagggg cacctccagg atatattgga catgatgaag
     4201 ggggtcaatt aactgaagcg gttcgtcgta atccatactc agttatttta ttagatgagg
     4261 ttgaaaaagc gcatactgac gtctttaatg tattattgca atttttagat gaaggccgtt
     4321 taactgattc taaaggacgt agcgttgatt ttaaaaatac tattattatt atgacaagta
     4381 atattggatc tcaagtttta ttagaaaacg taaaagagac tggtgaaatt acagaatcaa
     4441 cagaaaaagc tgttatgaca agtttaaatg catatttcaa accagaaatt ttgaatcgta
     4501 tggatgatat cgtattattt aaaccattat ctattgatga catgagtatg attgtagata
     4561 aaatcttaac gcaattaaat ataagattat tagaacaacg aatctcaatt gaagtttctg
     4621 atgatgctaa agcttggcta ggtcaagaag cttatgaacc tcaatacggt gcaagaccat
     4681 taaaacgttt tgtacaacgc caaattgaaa caccattagc acgtatgatg attaaagagg
     4741 gattcccaga aggtacaacg attaaagtta atttaaattc agacaataac ttaacgttta
     4801 atgttgaaaa aattcatgaa taaaattgaa ccaagaatgt gatgattaat cgcattcttg
     4861 gttttttaga tttgaaattt attataattg ttcattggca atatactttt taaatgattt
     4921 aataaacacc aata
//
LOCUS       DAMZLE010000158         4873 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMZLE010000158 DAMZLE010000000
VERSION     DAMZLE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.161"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(23..1216)
                     /gene="sqr"
                     /locus_tag="POB92_002395"
     CDS             complement(23..1216)
                     /gene="sqr"
                     /locus_tag="POB92_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HDI6579859.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(1235..2569)
                     /gene="cstB"
                     /locus_tag="POB92_002396"
     CDS             complement(1235..2569)
                     /gene="cstB"
                     /locus_tag="POB92_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDI6579860.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(2600..3667)
                     /gene="cstA"
                     /locus_tag="POB92_002397"
     CDS             complement(2600..3667)
                     /gene="cstA"
                     /locus_tag="POB92_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDI6579861.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            3804..4064
                     /gene="cstR"
                     /locus_tag="POB92_002398"
     CDS             3804..4064
                     /gene="cstR"
                     /locus_tag="POB92_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDI6579862.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            4064..4819
                     /locus_tag="POB92_002399"
     CDS             4064..4819
                     /locus_tag="POB92_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6579863.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIIQLIL"
BASE COUNT         1492 a          873 c          696 g         1812 t
ORIGIN      
        1 atttattgtt ttctgtactt tattatatta atccttttag catgccgtac caatacattt
       61 taggtaataa atctttctta aatatataca tacttctacg ttctttggcc tgattaaacg
      121 gcattgtttc tttagtattt ttattataat caaactctgc aagtattaac ctattatatc
      181 cagtaacaat agggcatgaa gtataaccat cataatgatg cgttaacatt tgattattca
      241 tcacttgcaa taaattatta gcgacgatag gtgcttgctt acgaatagct gcgcctgttt
      301 ttgaagtagg tacatttgaa gcatcaccaa gtgcaaatac attagagtag cttttatgct
      361 gtaatgtggt tgggttaaca tctacccaac cctcactatc tgaaagtgta ctttctttta
      421 ctacatctaa gggacccata ggtggtgtta catgtaacat atcataactt attgtttttc
      481 tatcgtatgc tttgatatgt tcgaatgtag ccactttttt gtcaccgtcg atttcaacaa
      541 ggttataatt ataattgact gttatatttc tttcttcaac aatcctttct aattctttat
      601 tatattttcc tacgtcaaat aaagcatctt ttggcgttgc atatatcaca ttagcgttag
      661 aacggatttt atgtttccta aaataatctt cagctaaata cataattttc ataggcgcac
      721 ctccacactt tataggagtg tttggatgcg taaaaatggc atttccttgt ttaaaattag
      781 aaatttggtt ccaagtttcg ttaacatagt caggtgaata gttagagcaa acaccatttt
      841 tacctatatt ttcttttagt cctttaattg aagaccaatt aatctgtaat cctggagcta
      901 ctactaaaaa atcataataa acgactgtat tatctcctaa aataacgcta ttattttcag
      961 gttgaaaact tgaaacagcc tgttttatcc agttagcacc ttcaggtata acactttcca
     1021 tatctttacg agaacttttc aaactagata cccctgcacc aaccaacgtc cataatggtt
     1081 gatagtaatg atggtctgct ggatctataa ttgctatttt ctcttttaag ttttgatttt
     1141 ttcttaatag tcttgatgct acggtaacac ctgctgtacc gccaccaata ataacaattt
     1201 gataatgctt attcattttt tccacatcct taaattattt taatgattct ggaaaatctt
     1261 gatagccttc tctaatattc accacatttt caaaaccttt gctttccaat atacccactg
     1321 caattgaact tctaacacct gactgacaat gtacatatat tttatcctct ttattaaaag
     1381 gaatattttc atttaataat ttaccatgcg gaatattaac tgcttgatct aagtgtccat
     1441 tattccactc ttcatcatta cgtacgtcta atacatgttc ttctttacct gtcatatcag
     1501 cgctatgaac ggactgggtt gaaatgcctg attttggcaa acgatagcct gctaccttat
     1561 caaacccaat taattgtaaa gtgtgtttcg ctttctcaac agtagattta tctccaatta
     1621 aatctatatc tttttcaaaa tctaagtacc aaccaatttg attaataaag tttttgttgt
     1681 atggtatatt gattgttcct tttgtgtggc caccgtgaaa ggcctctttg ctacgaagat
     1741 caaatgctac tctcttatta tctaaactag gataaacatc atatgattga tataaattca
     1801 taccaaactg attaacttgt ttcatttgtg caaaatgatg cggtggtgct ggttgatttg
     1861 atgttaatga ttcaataaat ttagtctcat caatttcatt aaatgcccag ttattaattt
     1921 tctcataacc tattgtagat ataggtatgg cacctaatgc tttaccacaa gggcttccag
     1981 caccatgacc cggccagatt tgaacatagt ctggtaaatt tttaatattt tgaacggact
     2041 catacatttg tttcgcgcta atttctgtag aaccctttat ttgaacagat ttttctaata
     2101 aatcaggtct acctatatca ccaacaaaaa taaagtcacc actaaataat cccatcggaa
     2161 cacttgaacc accacctaaa tcagtgagta aaaagctaat actttcaggc gtgtgtcctg
     2221 gagtatgcag aacttctaat ttaacattgc ctacttgaat gatatctcca tgtttaacaa
     2281 attgtgtttt tgatggcata tttttatacc ctaatgcatc ttcaccttcg ccagacacat
     2341 atatatttgc atttaagcgt ttagccacat cacgaattcc tgaagcaaaa tcagcatgaa
     2401 tatgtgtttc tgtagcttgt gtaattgtta aaccttcaga atctgcaact tctatatatt
     2461 tcgataaatc acgaacaggg tctattatta ttgcctctcc tgtacgttga caacccacta
     2521 aatatgatgc ttgagataaa tgattatcgt aaaactgttt aaaaaacatg ataacaactc
     2581 cttttattaa tagatttaat tagataaata aattatgatt cgcattttca gtagcaccaa
     2641 tataagtgcc tacaccaccg tactcaactt catctctaag ttcttctttc tgaataccca
     2701 tgacatccat actcatcgta caagcgatta atttgatatt ttgctcaata gcttgattga
     2761 taagtgttgg taatgaatca acatttttct ttttcattac gtagcgcatc atcatatttc
     2821 ctaaaccaaa catattcatt ttggaaagag gcattcgtat attctttttg ggcaacatta
     2881 aatcaaacat ttttgcaata ccttgctttt taacattaac tgtttgcact ttttttaatg
     2941 cattaagccc ccaaaaagta aagaagatag ttacatcttt tccagcagct ctagcaccat
     3001 ttgcaataat caacgctgct acagctttgt ctaattctcc actaaataat acaattgtag
     3061 tacctttagc agaataattt atatctaaat cttttgcttt ttctttttga ataatcgcat
     3121 taattccatt gttattttca tcaagcttaa ctaaagtatg ccttgtttgt ttcacccaac
     3181 ttttaatgtc actagggaat ccagggtctg tgactttgac ttcaatttgg tcacctactt
     3241 caatattctt catttcttga ctaattttta caattggccc tggacattga agaccacgat
     3301 agttaaattg tttacgatct gctttaattt ctatactttt atattcttct tgtgtatcat
     3361 aactatcgtt ttcttcttca taagctttat aaccaccatc aagatttata acgttataac
     3421 cttgtttagc taaatattta ctagcattag cacttctgtt accacttcta cagattacat
     3481 aataggtttt atttttttct ttattgaatg actcaatctc atcaacagga tgaagtatgg
     3541 aaccattgat atgtcctaat gcatactcct cttctgttct aacgtcaatt aattgccctt
     3601 gcttggctag tttttccaat tctgctttac taaattcatc gataaacttt tctccgtatt
     3661 gtttcataat aacctcccta aaaatacctg taggggtatt ttaaacagtt ggatggaact
     3721 tgtcaaatac ctctaggggt atttgacaca tttgtgttta tctattaata tatttttatt
     3781 caattaaaag tggaggtatg actatgaatt atgataaaaa aatgattaat cgtattaata
     3841 gaatacaagg gcaactaaat ggaattatta aaatgatgga ggaaggaaaa gactgtaaag
     3901 atgtcattac acaaataagt gcatcaaaga gttcactcca acgcttgatg ggtatcatta
     3961 ttagtgagaa tttaatagaa tgtgtaaaag cagctgcgga tgatgaagaa agctcccaag
     4021 agttaattaa tgaagctgta aacttattgg tgaaaagtaa gtaatggata ttgttaatat
     4081 agttatcatg ttactcattg gggtattcgg tgggttcata tctggtctag taggcgtagg
     4141 tggtgctatc attatttatc cagctatttt attattacca ccattattcg gagcccctgc
     4201 atatagcgca tatatcgcat caggtcttac ttctagccaa gtctttttca gtacattaag
     4261 tggctcatta aaagctcgaa aaaaaacaga gttctcgcca caattagttc tttatatggg
     4321 tggcggcatg attataggga gtatgttagg tgcatttcta gcaaatttat ttgatgctac
     4381 atttgtaaat acggtatata tcattattgc tttacttgca ttaacattaa tgtttattaa
     4441 agtgaaacct agttccgaaa agtcttcatt taataaatat ttattagtta tcattggcct
     4501 tttcattggt attatttcag gtatagtagg tgcaggtggt gccttcatta ttattcctat
     4561 attattggtt ttattcaaat taccaatgaa tactgtagtc gctaatagta tagtaattgc
     4621 ttttatatct tcaattggtg cttttgtcat caaacttatt caaggttata tccctttata
     4681 tgacgcctta ttccttataa tagggagtat tatatttgct ccaatagggt taaaattaag
     4741 taaaaaagtc cctaacataa tacaaaaatg gattatcagt attttaatcg tttttgctat
     4801 aattcaactc attttataaa actaatcctt ttaactcaga cagcaatttc ccgaaaaata
     4861 atacctctta ttt
//
LOCUS       DAMZLE010000159         4860 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMZLE010000159 DAMZLE010000000
VERSION     DAMZLE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.162"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            84..1544
                     /gene="ebpS"
                     /locus_tag="POB92_002400"
     CDS             84..1544
                     /gene="ebpS"
                     /locus_tag="POB92_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDI6579864.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKVSQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            1669..1785
                     /locus_tag="POB92_002401"
     CDS             1669..1785
                     /locus_tag="POB92_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579865.1"
                     /translation="MLFNKVLSRILAYQLNFLHSHQKDKTPQSTNLTRKNKK"
     gene            1946..2932
                     /locus_tag="POB92_002402"
     CDS             1946..2932
                     /locus_tag="POB92_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDI6579866.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(3047..4015)
                     /locus_tag="POB92_002403"
     CDS             complement(3047..4015)
                     /locus_tag="POB92_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI6579867.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDTNPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            4092..4751
                     /locus_tag="POB92_002404"
     CDS             4092..4751
                     /locus_tag="POB92_002404"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI6579868.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
BASE COUNT         1847 a          759 c          884 g         1370 t
ORIGIN      
        1 attttgaaaa atatacataa acatatgtca tgtgggtata ttttatgtaa aatcattgta
       61 atagaataga aaggaagatg gctatgtcta ataattttaa agatgacttt gaaaaaaatc
      121 gtcaatcgat agacacaaat tcacatcaag accatacgga agatgttgaa aaagaccaat
      181 cagaattaga acatcaggat acaatagaga atacggagca acagtttccg ccaagaaatg
      241 cccaaagaag aaaaagacgc cgtgatttag caacgaatca taataaacaa gttcacaatg
      301 aatcacaaac atctgaagac aatgttcaaa atgaggctgg cacaatagat gatcgtcaag
      361 tcgaatcatc acacagtact gaaagtcaag aacctagcca tcaagacagt acacctcaac
      421 atgaagagga atattataat aagaatgctt ttgcaatgga taaatcacat ccagaaccaa
      481 tcgaagacaa tgataaacac gagactatta aagaagcaga aaataacact gagcattcaa
      541 cagtttctga taagagtgaa gctgaacaat ctcagcaacc taaaccatat tttgcaacag
      601 gtgctaacca agcaaataca tcaaaagata aacatgatga tgtaactgtt aagcaagaca
      661 aagatgaatc taaagatcat catagtggta aaaaaggcgc agcaattggt gctggaacag
      721 cgggtgttgc aggtgcagct ggtgcaatgg gtgtttctaa agctaagaaa cattcaaatg
      781 acgctcaaaa caaaagtaat tctgacaagt cgaataactc gactgaggat aaagtgtctc
      841 aagataagtc taaagatcat cataatggca aaaaaggtgc agcgatcggt gctggaacag
      901 caggtttggc tggaggcgca gcaagtaaaa gtgcttctgc cgcttcaaaa ccacatgcct
      961 ctaataatgc aagccaaaac catgatgaac atgacaatca tgacagagat aaagaacgta
     1021 aaaaaggtgg catggccaaa gtattgttac cattaattgc agctgtacta attatcggtg
     1081 cattagcgat atttggaggc atggcattaa acaatcataa taatggtaca aaagaaaata
     1141 aaatcgcgaa tacaaataaa aataatgctg atgaaagtaa agacaaagac acatctaaag
     1201 acgcttctaa agataaatca aaatctacag acagtgataa atcaaaagag gatcaagaca
     1261 aagcgactaa agatgaatct gataatgatc aaaacaacgc taatcaagcg aacaatcaag
     1321 cacaaaataa tcaaaatcaa caacaagcta atcaaaatca acaacagcaa caacaacgtc
     1381 aaggtggtgg ccaaagacat acagtgaatg gtcaagaaaa cttataccgt atcgcaattc
     1441 aatactacgg ttcaggttca ccggaaaatg ttgaaaaaat tagacgtgcc aatggtttaa
     1501 gtggtaacaa tattagaaac ggtcaacaaa tcgttattcc ataatataac tatataaatt
     1561 gtaactgaac tgctatatac aaacgtgcat attaaaagta tctctaaata ttttggagaa
     1621 cttaaattgc gcttataagt atgtagcggt tttttcattt ttcaaagttt gttatttaac
     1681 aaggtgttgt ctcgaatatt ggcatatcaa ttaaattttt tacatagtca tcaaaaagat
     1741 aaaacaccac aatcaacaaa tttaacgagg aagaataaaa aataaatcaa catattaaat
     1801 tgtagtgtta ttcaactccg tagctaacaa ttctctatgc atattaaaca aattgtcgaa
     1861 aatatatcat aaatcttcaa gcacagactt agcgcatcaa tcactgaact gttataatag
     1921 tttgggatta aaggaggccg aaacaatgca aaaagttgaa agtatcataa ttggtggagg
     1981 gccatgcgga ttaagtgcgg ctattgaaca aaaaagaaaa ggtattgata ccttaattat
     2041 tgaaaagggt aatgtcgttg aatcaatata taattatcct actcaccaaa catttttctc
     2101 atcaagtgat aaattaagta ttggggacgt accgtttatc gttgaagaaa gtaaaccaag
     2161 acgtaatcaa gcgctagttt attaccgaga agttgtaaaa catcatcaat taaaagtaaa
     2221 tgcatttgaa gaagtattaa ctgttaaaaa aatgaataat aaatttacaa ttactacgac
     2281 gaaagatgtt tatgaatgtc gatttttaac aatcgcgaca ggctattatg gtcagcataa
     2341 tacattagag gttgaaggtg cggatttacc taaagtgttc cattatttta aagaggcaca
     2401 tccgtatttt gatcaagatg ttgttattat tggtggtaag aattcggcta tcgatgctgc
     2461 tttggagttg gaaaaagctg gtgctaacgt gacggttcta tatcgtggtg gagattattc
     2521 gccttcaatt aaaccgtgga tacttccaaa tttcacagca ttagtaaatc atgaaaaaat
     2581 tgacatggaa tttaatgcta atgttaccca aataactgaa gatactgtga cttatgaagt
     2641 aaatggtgaa agtaaaacga tacacaatga ttatgttttt gcgatgattg gttatcatcc
     2701 cgattatgaa tttttaaaat ctgtaggtat tcaaattaat acaaatgaat ttggaacagc
     2761 gcctatgtat aataaagaga catacgaaac aaatatcgaa aattgctaca ttgcaggtgt
     2821 gattgctgca gggaatgatg cgaataccat ttttattgaa aatggtaaat tccacggtgg
     2881 cattattgct caaagcatgc tagctaagaa acaaacgccg ttagaatcat aaaaataaag
     2941 gtctatgtac taaatatctt agttatacaa cgactgacat tcatgatatg tcaaatgagg
     3001 ttgatgacta atgattgtac atagaccttt ttatgttacg taatcattat aattcaaaat
     3061 atgatttgat ttcagcttta tctaaattgt tgcttaacgc aactaataat tttaatcttg
     3121 cttttggacc attcaaaccg ttagaaaaaa taaaaccttg ttgtgcgagt tggtaaccac
     3181 caccatcgta tgcgtaagtt ggactcacaa taccattaaa ggaacgtgaa actagcacaa
     3241 taggtatatt taaagatact aattgttgaa tgccttctaa tgcgcttgga ggtatgttgc
     3301 cttgtcctaa cgcttcaata accataccat cgataccttc tcgactataa aaactaaaaa
     3361 tgtcacctgg catacccata tatgctttta ctaatggtac atttagtttg tcattgacat
     3421 tttccaatgc ttgttggcga tatggcatat gatggaattg cacacgatcc tttgtcaata
     3481 cacctagcgg accatgattt ggactttgaa atgtgtttgt attagacgta tgtgttttgg
     3541 taacattacg cgccgtatga atttcatcat taaatacaac catcacgcct ttatgacggg
     3601 ccttttcatc agaggcaacg cgaatagcgg aaatataatt atataatccg tcagaaccaa
     3661 tttcattaga cgagcgcatt gcgccagtaa taacaacagg ttgctcgata cctaatatta
     3721 aatcaagtaa aaaggcagtt tcttctaacg tatcggtacc atgcgtgata acgaaaccat
     3781 cataatattt atttgttact gcttctaaaa taatatcctt taattgttta acatgttgga
     3841 ttgtcatatg aggtgatggt acattaaaag gatttaattc atctatttgt gcatattgat
     3901 ttatgacatc ttgatgcatt gaaatagggt tggtatcatt tgttactact ttattagatt
     3961 ggtcttgtga catactaatg gtgccaccag tatgaataac aagtagatgt ttcatatatt
     4021 tcctcctata tttaatttac ctaattatga taaaatatta ttcataaaac gacaaggaag
     4081 ggaaatgacg catgaaagcc attaatattg cattagatgg tccagctgct gccggaaaaa
     4141 gtacaattgc gaaacgtgta gccagcgaac tatcaatgat ttatgtcgat acaggagcaa
     4201 tgtatcgtgc attaacatac aaatatttaa aattaaacaa aactgaggac tttgcaaaac
     4261 tagttgacca aacaacatta gatttaactt ataaagcaga taaaggtcaa tgtgtcattt
     4321 tagataacga agatgtaaca gactttttaa gaaataatga tgtgacgcaa catgtttcat
     4381 acgttgcatc taaagagcca gtacgttcat tcgccgttaa aaaacaaaaa gagttagctg
     4441 cagaaaaagg tatcgtaatg gatggtcgcg atatcggaac tgtagtgcta ccagatgcag
     4501 atttaaaagt atatatgatt gcatcagttg aagagcgagc agaaagaaga tataaagata
     4561 atcaattaag aggtatcgaa tcaaattttg aagatttaaa acgtgatatt gaagctcgtg
     4621 atcaatatga catgaaccgt gaaatatcac cattaagaaa agcagatgat gcagtgacat
     4681 tagatacgac aggcaagtcg attgaagaag ttactgacga aattttagcg atggtgagtc
     4741 aaattaaata attaagaagc taacattaat aaaatttata attacttgat gaacggaaaa
     4801 ggctgttttt attctaatcg aatagaaatg gtcttttgtt aatttataaa cgaataacga
//
LOCUS       DAMZLE010000160         4851 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMZLE010000160 DAMZLE010000000
VERSION     DAMZLE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.163"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            40..1320
                     /locus_tag="POB92_002405"
     CDS             40..1320
                     /locus_tag="POB92_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDI6579869.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEI
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            1326..2387
                     /locus_tag="POB92_002406"
     CDS             1326..2387
                     /locus_tag="POB92_002406"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDI6579870.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2389..3303
                     /locus_tag="POB92_002407"
     CDS             2389..3303
                     /locus_tag="POB92_002407"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDI6579871.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIAIQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3361..4164
                     /locus_tag="POB92_002408"
     CDS             3361..4164
                     /locus_tag="POB92_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6579872.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(4457..4771)
                     /locus_tag="POB92_002409"
     CDS             complement(4457..4771)
                     /locus_tag="POB92_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579873.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4746..4850)
                     /locus_tag="POB92_002410"
     CDS             complement(4746..4850)
                     /locus_tag="POB92_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579874.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT         1747 a          661 c          944 g         1499 t
ORIGIN      
        1 catttgtttt tctcaaaagc acaaacggag gtacataaaa tgaaaaaatt aaatatagca
       61 ttattaggat taggtactgt cggatctggt gttgttaaaa tcatcgaaga gaaccgacag
      121 caaattcaag atacattaaa taaagatatt gtcataaagc atattcttgt tcgagataaa
      181 tctaaaaaga gaccgctaaa tattagccaa tatcatttaa ctgaagatgt taatgaaatt
      241 ttaaatgatg attcattaga tattatcgtt gaagtcatgg gaggaattga accaactgta
      301 gattggttaa gaacagcact taaaaataaa aaacatgtta ttaccgcaaa taaagattta
      361 ttagcagtac atcttaaact tttagaagat ttagcagaag aaaatggtgt agctttaaag
      421 tttgaagcga gtgtagcagg tggtattccg atcgtaaatg ccataaataa tggtttgaat
      481 gcgaataata tttcaaaatt tatgggaatt ttaaatggta cctctaattt tattttatct
      541 aaaatgacta aagagcaaac gacatttgag gaagcacttg atgaagcgaa aagacttggt
      601 tttgctgaag cggatccaac tgatgatgta gaaggggtag atgcagcgcg taaagttgtc
      661 attacatcat atttatcatt taaccaagtc attaaattaa acgacgttaa acgaagagga
      721 attagtggcg taactttaac tgatattaat gtagccgatc aactggggta taaaattaaa
      781 ttgattggta agggaatata tgaaaatggc aaagttaatg catcggtaga accaacgtta
      841 attgataaaa agcatcaatt agcagctgta gaggatgaat ataacgcgat ttatgtcatt
      901 ggtgatgccg ttggtgacac gatgttttat ggaaaaggag caggcagttt agcaacaggt
      961 agtgccgttg tcagtgattt attgaatgta gcattattct ttgaatcaga tttacacaca
     1021 ttgccaccac attttgaaat aaagacagat aaaacacggg aaatgatgga ttcagatgca
     1081 gaaattaata ttaaagaaaa atccaatttc tttgtagtag tgaatcatgt caaaggttca
     1141 attgaaaatt ttgaaaatga gttaaaggca atattaccat ttcaccgatc attaagagtt
     1201 gcaaattacg ataatcaatc atatgccgct gttatagttg gattggaatc atcaccggaa
     1261 gaattaatca ctaagcatgg atacgaagtt gacaaagtat acccagtaga aggagtttaa
     1321 ttataatgag aagatggcaa ggattagtag aagagtttaa agcacattta ccagtaaatg
     1381 aaaatacacc aaaattaaca ttgaacgagg gaaatacacc actcattcat tgtgaaaata
     1441 tgtctaaaat actaggtata gatttatatg tgaagtatga aggtgccaat ccgacaggtt
     1501 catttaaaga tcgcggtatg gtaatggctg tgacaaaagc aaaagagcaa ggtaagaaaa
     1561 ttgtaatatg cgcttcgact ggaaatacat cagcgtctgc agcagcatat gcagcgagag
     1621 caggtttaaa agctatcgtc gtaataccag aaggtaaaat tgcattaggt aaattgtcgc
     1681 aagcagtaat gtatggtgca gaaatcgttt ctattgaagg aaactttgat gaagctttag
     1741 aaattgtaaa agaaattgca aaaagtggcg aaatagagct tgtaaactct gtcaatccat
     1801 ttagaatcga agggcaaaag acaggctcat ttgaaattgt acaacaatta gacggtgaag
     1861 cacctgatat tttagcgatt cctgtaggta atgcaggtaa tattactgca tattggaaag
     1921 gctttaaaga atatcatgaa gctaaaggtt cacaattgcc gaaaatgttt ggcttccaag
     1981 ctgaaggcgc atcaccaatt gttcaaaata aagtcattaa aaatcctgaa acgattgcaa
     2041 ctgctattcg aattggtaat cctgctagtt gggataaggc gactaatgct cttaaagaat
     2101 caaatggatt aatagatagt gttactgatg atgaaattct agaagcatat cagttaatga
     2161 caactaaaga aggtgtcttt agtgaaccag cgagtaatgc ttctattgca ggtttaatta
     2221 aattgcatag acaaggtaaa ttacctcaag gtaaaaaagt agttgctatt ttaactggta
     2281 atggattaaa agatcctgat actgctattt cactactaga taatccgata aagccattgc
     2341 caaatgataa agatagcatt atcgattata ttaaaggagc tttataacat gtcgaatgtt
     2401 ttggagttaa caattcctgc atcaacagcc aaccttggag ttggctttga ttctataggt
     2461 atggctttag ataaattttt gcatctgtct gtaaaggaaa catcagggac aaaatgggaa
     2521 tatattttcc atgatgatgc atctaagcaa ttgcctactg acgaaacaaa ctttatttat
     2581 catgtagcac aacaagttgc ttctaaatat agtgttgact tgcctaattt atgtatcgaa
     2641 atgagaagtg atattccatt ggcaagaggg ttaggttcgt cagcttctgc tttagtagga
     2701 gctatatata tcgcaaatta ttttggtgat atccaactgt ctaaacatga ggtattacaa
     2761 ttagcgactg aaatcgaagg acatcctgat aatgttgcgc cgaccattta tggtggttta
     2821 atcgctggat attataatga tgtctcgaaa gaaacgtcag ttgcacatat cgacatacca
     2881 gacgtggatg tgattgtaac gataccaact tatgaactaa aaacagaagc atcaagacgt
     2941 gctttaccac aaaaattaac acatagtgaa gcggttaaaa gtagtgcaat tagtaataca
     3001 atgatttgtg cattagcaca gcacaattat gaattagcag gtaaactcat gcaacaagat
     3061 ggctttcatg aaccgtatcg tcagcattta attgctgaat ttgatgaagt gaaaacaatt
     3121 gctattcaac ataatgccta tgcaactgta attagtggtg ctggaccaac tattttaata
     3181 tttagtcgta aagaaaatag tggggaattg gttcgctctt taaatagtca ggtagtatca
     3241 tgccattctg aattggtcga tattaatatc agtggtgtta aagaacgaat tgtataccaa
     3301 tagacgcttt atattgtaaa atagtattaa atgcagaata gagaggagat ttaatgcgat
     3361 atgacaaatt ataaagttgt cgttttagat atggatgaca cattgctaaa ttcagataat
     3421 gtgatatcag aagaaactgc aaattattta acagcaattc aagatgaagg ttattatgtt
     3481 gttttagcat ctggtagacc tactgaaggt atgattccaa ctgctagaga tttaaaatta
     3541 cctgaacatc atagctatat tattagttat aacggtagta aaacgattaa catgactaat
     3601 gaagaagtag aagtaagtaa atcgattggt aagcaagatt tcgatgaaat tgtagattat
     3661 tgtcgagata gaggcttttt cgttcttaca tatcatgatg gtcaaattat ttacgacagc
     3721 gaacatgagt atatgaatat tgaagcagaa ttaacaggtt taccgatgaa acgtgttgat
     3781 gatatcaaag cgtatattca aggcgatgta cccaaggtca tgggtgtaga ttatgtagcg
     3841 aatattacag aagctagaat tgatttgaat ggtgtgttca atgataatgt agatgctacg
     3901 acaagtaagc cattcttctt agaatttatg gccaaagacg tttcaaaagg taatgcaatt
     3961 aaagcgttat gtcacaaatt gggatattcg gtggatcaag tcattgcttt tggtgatagt
     4021 atgaatgata aatcaatgtt tgaagtcgca ggtctagcta ttgctatggg gaatgcatca
     4081 gatgaactta agcaatatgc aaatgaagtt acgttggatc ataatgaaaa tggtattcca
     4141 catgcgctca aaaaattgtt ataaatttta aaataagcct taacacatga tatttgaata
     4201 agatatcttg tggttaaggc tttttatttt tgtgaaaatg acttcagtta tactatggag
     4261 gatttgaaat acatatttta gattagtaat gatatcaaac gaatagagta aatgtatatt
     4321 ttttgaataa atcaagtatt aactagtcac tgaaggtaga taaataaatt tgatcattct
     4381 gaataataac gaatgcatta aaaaatgcag tgacaatcta acattaattg ttaaaatcac
     4441 tgcaagcatc ttgattttat ttagctgttt gtgtccagtc atgcttacct ctgaaacgtt
     4501 ttacatgagc atatatttgg tcagcagtat aatctaaatt tgtataaact gtaatactga
     4561 acgctgattg gttttcagca aatgattctt cggaaaataa ttcctttaag tgcataaata
     4621 aatcattcat ttgtttatgg tttaaaccgg gatattccct taaagttctc tttaataatt
     4681 gtccgttctt ttcttctaat gcttgaacta caataacaaa tctttcatct tgctttagta
     4741 catcatcaaa taaatttgtt tgtccaatca ttgaatttca cccctgaaaa gttattgtta
     4801 tctatattat acacgatatg caaaatatct aaaataaaga atggtaacat g
//
LOCUS       DAMZLE010000161         4635 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMZLE010000161 DAMZLE010000000
VERSION     DAMZLE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.164"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            17..769
                     /gene="sarS"
                     /locus_tag="POB92_002411"
     CDS             17..769
                     /gene="sarS"
                     /locus_tag="POB92_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDI6579875.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            1190..2572
                     /gene="spa"
                     /locus_tag="POB92_002412"
     CDS             1190..2572
                     /gene="spa"
                     /locus_tag="POB92_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDI6579876.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNKKPGKEDGNKPGKEDNNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAAD
                     NKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGA
                     ALLAGRRREL"
     gene            complement(2901..4493)
                     /locus_tag="POB92_002413"
     CDS             complement(2901..4493)
                     /locus_tag="POB92_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6579877.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPVRVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFVIDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLKESEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1754 a          846 c          669 g         1366 t
ORIGIN      
        1 acaaggagat aaaacaatga aatataataa ccatgacaaa attagagatt ttataatcat
       61 tgaagcatat atgtttcgtt ttaagaaaaa agtcaagcct gaagtcgata tgactataaa
      121 agaatttata ttactgactt atttatttca tcagcaagaa aacacacttc catttaagaa
      181 gattgtttca gatttatgtt ataaacaatc ggatttagta cagcatataa aagtacttgt
      241 gaaacattca tatattagta aagttcgaag taaaattgat gagcgtaata cttacatttc
      301 aatatctgaa gaacaacgag agaaaattgc agaacgtgtt acattgtttg atcaaatcat
      361 taaacaattt aaccttgcag atcaaagtga atcacagatg ataccaaaag atagtaaaga
      421 atttctaaac ttgatgatgt atacaatgta tttcaagaat attatcaaaa aacatctaac
      481 attaagtttt gtagaattca caattctagc tattatcact tctcaaaata aaaacatcgt
      541 tcttcttaaa gatttaattg aaacaatcca ccataaatac cctcaaactg ttagagctct
      601 caataattta aaaaagcaag gctatctaat aaaagaacgc tcaactgaag atgaaagaaa
      661 aattttaatt catatggatg acgcgcagca agaccatgct gaacaattat tagctcaagt
      721 gaatcaatta ttagcagata aagatcattt acatcttgtt tttgaataat atctctatta
      781 cgcaagtgtg ctgtattcta aagtgcactt gtgttttcta ttttttaata aaacctcagc
      841 acataatgaa caactttcta ttttctatat cacttaaaac catttccgaa attaaacctc
      901 agcacattca aagccccact ttattcttaa aaatattttt taactcatat gtattaaacc
      961 gctttcatta taaaaaatat ctctatattt tatctgtttt tattaatcga aatagcgtga
     1021 ttttgcggtt ttaagccttt tacttcctga ataaatcttt cagcaaaata tttattttat
     1081 aagttgtaaa acttacattt aaatttaatt ataaatatag attttagtat tgcaatacat
     1141 aattcgttat attatgatga ctttacaaat acatacaggg ggtattaatt tgaaaaagaa
     1201 aaacatttat tcaattcgta aactaggtgt aggtattgca tctgtaactt taggtacatt
     1261 acttatatct ggtggcgtaa cacctgctgc aaatgctgcg caacacgatg aagctcaaca
     1321 aaatgctttt tatcaagtgt taaatatgcc taacttaaac gctgatcaac gtaatggttt
     1381 tatccaaagc cttaaagatg atccaagcca aagtgctaac gttttaggtg aagctcaaaa
     1441 acttaatgac tctcaagctc caaaagctga tgcgcaacaa aataacttca acaaagatca
     1501 acaaagcgcc ttctatgaaa tcttgaacat gcctaactta aacgaagcgc aacgcaatgg
     1561 tttcattcaa agtcttaaag acgatccaag ccaaagcact aacgttttag gtgaagctaa
     1621 aaaattaaac gaatctcaag caccgaaagc tgacaacaat ttcaacaaag aacaacaaaa
     1681 tgctttctat gaaatcttga acatgcctaa cttgaacgaa gaacaacgca atggtttcat
     1741 ccaaagctta aaagatgacc caagtcaaag tgctaaccta ttgtcagaag ctaaaaagtt
     1801 aaatgaatct caagcaccga aagcggataa caaattcaac aaagaacaac aaaatgcttt
     1861 ctatgaaatc ttacatttac ctaacttaaa cgaagaacaa cgcaatggtt tcatccaaag
     1921 cttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga
     1981 tgcacaagca ccaaaagctg acaacaaatt caacaaagaa caacaaaatg ctttctatga
     2041 aattttacat ttacctaact taactgaaga acaacgtaac ggcttcatcc aaagccttaa
     2101 agacgatcct tcagtgagca aagaaatttt agcagaagct aaaaagctaa acgatgctca
     2161 agcaccaaaa gaggaagaca acaacaaacc tggtaaagaa gacaacaaaa aacctggtaa
     2221 agaagatggc aacaagcctg gcaaagaaga caacaacaaa cctggtaaag aagacggcaa
     2281 cggagtacat gtcgttaaac ctggtgatac agtaaatgac attgcaaaag caaacggcac
     2341 tactgctgac aaaattgctg cagataacaa attagctgat aaaaacatga tcaaacctgg
     2401 tcaagaactt gttgttgata agaagcaacc agcaaaccat gcagatgcta acaaagctca
     2461 agcattacca gaaactggtg aagaaaatcc attcattggt acaactgtat ttggtggatt
     2521 atcattagcg ttaggtgcag cgttattagc tggacgtcgt cgcgaactat aaaaacaaac
     2581 aatacacaac gatagatatc attttatcca aaccaatttt aacttatata cgttgattaa
     2641 cacattctta tttgaaatga taagaatcat ctaaatgcac tagcaacatc ttttgttgct
     2701 cagtgcattt tttattttac ttacttttct aaacaacttc tgaaacgcct caacactttc
     2761 tactctaatt acatatacga catttttaga cattaaaaaa tcgaactaga caagatgctc
     2821 aatgcatttc gtactagttc gattcatgaa taattagatt taaaatgtca tttgaatcca
     2881 agtgacaaca ttatttatat ttagaatatt aacgttagta taaacgtcca aacacaaata
     2941 aaagcaacaa atataatact gtattttagc gtcattttta ataatgcaga ttcttcacca
     3001 acttttttaa cagctgcagt cgcaatggca attgattgtg gtgaaataag tttcgctgct
     3061 acaccacctg cagtgttagc tgccacaagt aatgaaccgc ttgttgaaat ttgttgtgct
     3121 actgtcgctt gaataggtgc gaataaagta ttgttattta caactgaacc agtcataaac
     3181 acaccaatcc aacctaatac tggagagaat aatgggaaaa ttgctccagc tttagcaata
     3241 ccttgtccaa ttgctacagt caaaccaccg tatgtcataa ctttagcaat agctaggata
     3301 gctgaaattg taaggatcgg taaccataat tctttaattg cttcgaccaa taaagcacct
     3361 gcacttttcc attttagctt cgtaattaaa attgtaataa ttactgttaa taaaatcgct
     3421 gtcccagttg caccaattaa atcgagacgc aacgcaattc ctttaggcga taaatcactc
     3481 acagtatttg gaattggcaa ttttattact aaactttcaa gtgcacctcc aggttggaat
     3541 aattttttga agaatggtgc actccatact aaaacaaagg cagttaaaat gacgaacgga
     3601 ctccaagcaa agacaatttc tttaggcgtt cgtttttgaa ttttatgttc tgattctttc
     3661 aacctgaaaa tgtttttcgg tttaaattta cgacaaacaa atgctaacac caccattgtt
     3721 gctagtgatg ggataatgtc tgctagttct ggaccatgga atattgttaa taataattgt
     3781 aatccagtat atgtaccact cactgttaaa atgacaggta aaatttcttt aatacctttc
     3841 atgccatcta taacgaatac taaaacaaat gggataataa agtttaaaat tggaagtgtt
     3901 aatgctgaat atctcgcaac atctaatgtt gtaacgcctc cacttaagtt aaacgtatca
     3961 ataatactaa ctggtaaacc aattgcacca aaggcacccg ccgcaccatt agcaattaag
     4021 cataacatcg ctgcttttaa tggttcaaat ccaagttgaa ttaataatac tgcacaaatc
     4081 gcaattggca caccaaatcc tgctgcacct tctaaaaatg cgttgaaaca aaatccaatt
     4141 aataatagtt ggattctttg gtcctctgaa atacttgcaa tactatcttg aataatagaa
     4201 aattgtcctg ttttaataga aactttatat aaccaaactg ccattaaaac gatatatcct
     4261 attgggaaaa taccggcaac aacgccttct gtaatcgcac ctgctgatac acgcactggt
     4321 aattcaaata caaataaagc cacaatcaat gtaacaacca aagttgtcaa tgctgcataa
     4381 atgcctttca ttttaaaaac ggttaagcat aataaaaata aaataatagg tactgctgca
     4441 actaaggctg ataatccaac attatcgaat ggatttacag taagtagtgt cataatgact
     4501 ccctctcttt atataaaata tttatcatcc tgattaatct acaacctatt tcaacttata
     4561 ttttgcgatg atcacatatt taaaatgtaa cactcctata tgtgacaggc aatcgaattt
     4621 ttacaaaaag ttcac
//
LOCUS       DAMZLE010000162         4559 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMZLE010000162 DAMZLE010000000
VERSION     DAMZLE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.165"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            245..1420
                     /gene="rpsA"
                     /locus_tag="POB92_002414"
     CDS             245..1420
                     /gene="rpsA"
                     /locus_tag="POB92_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI6579878.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1642..2952
                     /gene="der"
                     /locus_tag="POB92_002415"
     CDS             1642..2952
                     /gene="der"
                     /locus_tag="POB92_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI6579879.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2969..3967
                     /locus_tag="POB92_002416"
     CDS             2969..3967
                     /locus_tag="POB92_002416"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI6579880.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4138..4410
                     /locus_tag="POB92_002417"
     CDS             4138..4410
                     /locus_tag="POB92_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDI6579881.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1643 a          609 c          905 g         1402 t
ORIGIN      
        1 ataaaacaaa atagtaagct atgctaattt ttatacaaaa aatatgtata aacatagcaa
       61 actgtattac tttgatacaa aaatggttgt aataaatatt tatcgatatg acgacttgaa
      121 tatgataaag tgacatattt atgtatatga ctatttcgca aaatgtaatc gaggtagaat
      181 ttcttgacaa ttctgtcagt ttataagatg ttataaatat gtagtgtata aggaggcaaa
      241 caagatgact gaagaattca atgaatcaat gattaacgat attaaagaag gtgacaaagt
      301 cactggcgag gtacaacaag ttgaagacaa gcaagttgtt gttcatatca acggtggtaa
      361 atttaatggg attattccta ttagtcaact atctacgcat catattgata gcccaagtga
      421 agttgtaaaa gagggcgacg aagttgaagc atatgtcact aaagttgagt ttgatgaaga
      481 aaatgaaact ggagcttaca tcttatctag aagacaactt gaaactgaga agtcttatag
      541 ttatttacaa gaaaaattag ataataatga aatcatcgaa gcgaaagtaa cagaagtagt
      601 taaaggtggt ttggttgttg atgtaggaca aagaggtttt gttccggctt cactaatttc
      661 aacagacttc attgaggatt tctctgtgtt tgatggacaa acaattcgta ttaaagttga
      721 agaattggat cctgaaaata atagagtcat tttaagccgt aaagcagttg aacaagaaga
      781 aaacgatgct aaaaaagatc aattattaca atctttaaat gaaggcgatg ttattgatgg
      841 taaagtagcg cgtttaactc aatttggtgc atttatagac attggcggtg ttgatggttt
      901 agtgcatgta tctgaacttt ctcacgaaca tgttcaaaca ccagaagaag tagtttcaat
      961 tggtcaagat gttaaagtta aaattaaatc tattgataga gatacagaac gtatttcatt
     1021 atcaatcaaa gatacgttac caacaccttt cgaaaatatt aaaggtcaat tccacgaaaa
     1081 tgatgtcatt gaaggtgtcg tagtaagatt ggcaaacttt ggtgcatttg ttgaaattgc
     1141 accaggtgta caaggacttg tacatatttc tgaaattgca cacaaacaca ttggtacgcc
     1201 aggtgaagtg ttagaacctg gtcaacaagt aaatgttaaa atattaggta ttgatgaaga
     1261 gaatgaaaga gtatcactat ctattaaagc aacattacca aacgaagatg ttgttgaaag
     1321 tgatccttct acgactaagg cgtacttaga aaacgaagaa gaagataatc caacaattgg
     1381 cgatatgatt ggtgataaac ttaaaaatct taaactataa tttaatattt aatagtcaac
     1441 tccacatgtt tatgattgca tgtggagtat ttttatgtaa caaaatatac tcggaatgat
     1501 aacgtggaca aatttaacta agtgtttaaa aagataaagt tttaagtgct gatttttatc
     1561 attacagtaa taaactcatt ttgaatacac agtctcatgt gatattatta aaaagatata
     1621 agaaagagag gaagttagct tatgactaaa cctatagtag ctattgtagg taggcctaat
     1681 gtaggtaaat ctacaatttt taatagaata gttggagaac gtgtttcgat tgtggaagac
     1741 acgccaggtg taacacgaga tcgtatttat tcttcaggtg aatggttaac acatgatttc
     1801 aatattattg atacaggtgg tattgaaatt ggtgatgcac cattccaaac acaaattaga
     1861 gcgcaggcag aaatcgccat agatgaagcg gatgttatta tttttatggt taacgtgcgt
     1921 gaaggattga cacaaagcga tgaaatggtc gctcaaattt tatacaaatc taaaaaaccg
     1981 gtcgtattag cggttaacaa agtagataat atggaaatgc gtacagacgt gtatgatttc
     2041 tattcattag gatttggtga accgtatccg atatcagggt cacatggttt aggtcttggt
     2101 gacttgttag atgcagttgt ttctcatttt ggtgaagagg aagaagatcc ttatgatgaa
     2161 gatacaattc gactatccat tattggacga ccaaacgtag gtaaatcaag tttagtaaat
     2221 gctattttag gtgaagatcg cgttatcgtt tctaatgttg cagggacaac gagagacgct
     2281 attgatacag agtatagtta tgatggacaa gattatgttt taatcgatac tgctggtatg
     2341 cgtaaaaaag gaaaagtata tgaatcaact gagaaatatt cagtattaag agctttaaaa
     2401 gcgattgaac gttcaaatgt tgttttagtg gtcatagatg cagaacaagg catcattgaa
     2461 caagataaac gtgttgcagg atatgcacat gaacaaggta aagcagtcgt gattgtcgta
     2521 aataaatggg atactgtgga aaaagatagt aaaacgatga agaaatttga agatgaagta
     2581 cgtaaagaat tccaattttt agattatgca caaattgctt ttgtgtctgc taaagaacgc
     2641 acaagattac gtacattatt cccttacatc aatgaagcaa gtgaaaacca taaaaaacgt
     2701 gttcaaagtt caactttaaa tgaagttgtt actgatgcaa tttccatgaa ccctacacca
     2761 acagacaaag gtagacgttt gaatgtcttt tatgcaacac aagttgctat agaaccaccg
     2821 acatttgttg tatttgttaa tgatgtagaa ttaatgcatt tttcttataa acgctattta
     2881 gagaatcaaa tccgtgccgc ttttggtttt gaaggtacac caattcatat tatagctcga
     2941 aagagaaatt aacgattggg ggataacaat gactaaaatt accgtttttg gtatgggaag
     3001 ttttgggaca gcccttgcca atgttcttgc agaaaatgga catgatgttt tgatgtgggg
     3061 taaaaatcaa gatgctgttg atgaattaaa tacatgtcat acaaataaaa agtatttaaa
     3121 atacgcgaaa ttagatgtta acatcatcgc tacttcagat atgaccaaag caattcaatt
     3181 tgcagatatt tacttaatgg ctttacctac taaagcaatg cgagaagttg cttctcaaat
     3241 taatgataag ctgacctcta aaaagacttt tatacatgtt gctaaaggta ttgaaaatgg
     3301 gacgtttaaa cgtgtgtcag aaatgattga agattctatt tcacctgaat ataatgcagg
     3361 tattggcgtg ttgtcagggc caagtcatgc ggaagaagtt gtagtcaagc aaccaactac
     3421 agttgctgct tcatcaaaag ataaaagtgt aagtaaatta acgcaagatt tatttatgaa
     3481 tgattatttg cgtgtgtaca cgaatgatga cttgattggt gttgaacttg gtggtgcatt
     3541 gaaaaatatc atcgcagtag caagtggtat cgtagctgga attggctacg gtgataatgc
     3601 aaaagctgca ttaatgactc gtggtttagc ggaaattagt agattaggtg aaaagttagg
     3661 tgccgatcct atgacatttc taggtttagg tggtatcggt gacttaatcg ttacttgcac
     3721 atcaacacat tctcgaaatt tcacattagg atataaactt ggacaaggtg aatcaatgga
     3781 tcaagcatta tctgaaatga atatggtcgt tgaaggtatt tatacaacta aatcagttta
     3841 tcatttagct aaagaaaaaa atgtggatat gccaattaca aatgcattat atagagtatt
     3901 atttgaaaat atctcagtaa aagaatgcgt aaaagattta atggagcgcg ataaaaaatc
     3961 tgaataaaat gtgtattatt acacatatac ggctaataat tacgtaaaat gaatagaaat
     4021 ggcttaaatt caacgttttt gccacataaa taattgcgat ttatgtaatg cttgtgttaa
     4081 agttttacca taatgatata aacatatcat tatcaaacct caggaggtga atgtctaatg
     4141 aacaaaacag atttaatcaa tgcagttgca gagcaagctg atttaactaa aaaagaagct
     4201 ggttcagcag tagatgctgt attcgaatca atccaaaact cacttgctaa aggtgaaaaa
     4261 gtacaattaa ttggtttcgg taactttgag gtacgtgaac gtgctgcacg taaaggtcgt
     4321 aaccctcaaa ctggtaaaga aattgatatc ccagcaagta aagttccagc attcaaagct
     4381 ggtaaagcat taaaagatgc tgtaaaataa ttttacttaa aaagccctga ataagggctt
     4441 tttattttgc ttttaatact tacaacctgt acataaattg taatgttctt ctaagttttt
     4501 aatctttgga acattcattg tttaagcgtt caaaatttta catgacataa tagttttga
//
LOCUS       DAMZLE010000163         4522 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMZLE010000163 DAMZLE010000000
VERSION     DAMZLE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.166"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..163
                     /locus_tag="POB92_002418"
     CDS             <1..163
                     /locus_tag="POB92_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6579882.1"
                     /translation="GNDHNDIVMLQHASSGYIIGPSEAYTHAILKLDKIKHINNNAQA
                     ICKVLKSYK"
     gene            complement(212..685)
                     /locus_tag="POB92_002419"
     CDS             complement(212..685)
                     /locus_tag="POB92_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI6579883.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(809..1510)
                     /locus_tag="POB92_002420"
     CDS             complement(809..1510)
                     /locus_tag="POB92_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579884.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPAKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(1503..2276)
                     /locus_tag="POB92_002421"
     CDS             complement(1503..2276)
                     /locus_tag="POB92_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579885.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(2263..3093)
                     /locus_tag="POB92_002422"
     CDS             complement(2263..3093)
                     /locus_tag="POB92_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579886.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(3086..4072)
                     /locus_tag="POB92_002423"
     CDS             complement(3086..4072)
                     /locus_tag="POB92_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579887.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISVSSDRHA"
     gene            complement(4243..4428)
                     /locus_tag="POB92_002424"
     CDS             complement(4243..4428)
                     /locus_tag="POB92_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579888.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(4376..>4522)
                     /locus_tag="POB92_002425"
     CDS             complement(4376..>4522)
                     /locus_tag="POB92_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579889.1"
                     /translation="LAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYGYEGAELRR
                     KDKK"
BASE COUNT         1579 a          803 c          612 g         1528 t
ORIGIN      
        1 tggtaatgac cacaatgata ttgtcatgtt acaacatgct agtagtggct atattatagg
       61 accatcagaa gcatacacac acgcaatatt gaaacttgat aaaatcaaac acatcaataa
      121 taatgcacaa gctatttgca aagtcttaaa atcatataaa taaaaaacac ccctatcaaa
      181 tgataatcat tatcaatcga taggggctat tttaataaaa ttcgtcctcg aacatttctt
      241 cctcttcatc taatccaaat aattctgcca tttctccatg ttcaattaac atgtttaaat
      301 atgcatcgcg gagttcttct tcactcatat cattaatcat ttctttaaga ctatcaatcc
      361 acatatttct gcgtaattga tagtcttctt caacttcgtt taacatcatt atatgtttat
      421 ttgctgcttc tggactagct gtaaagagta atgcaatcat atgtttacat atcactcgtc
      481 ttccatcagc atgaggacaa ttacatttgg attttctagg atgttccata tcaatataac
      541 aacgatatac tttgttgcca ctgcccttta cttcagcctc atgctgcgtt tctgaaaatg
      601 attttaagtt aatgacgcat tcactttgat aataattaaa gcctctttct atagaacgaa
      661 tacttgcaat atcaagtaat cccattaatg atactccttt ttattattat ttttaaataa
      721 agaaaataaa ataaataagt gtctagatta atatacttaa ttcatctata ttttataaca
      781 agtctagaat tatcgcattc ttaaatagct aatatgaaaa tgcttgcact aattcttttg
      841 tataagggtg tctatcaaca ttaaataatt cctctattgc aaaatcatcg actatcatgc
      901 catccttaag aacgataatt ctattaacta agcgttgtaa cacggataaa tcatgagaaa
      961 taacgataaa atgatttaag ttcgtaatcg tttgcgcttt taatatattt attacatttt
     1021 gttcagctat aacatctaaa tttgaagtta tctcatcaca tattaaaacg cgaggctgtg
     1081 ctaataacga acgcatgaca ttaaatcttt gtaattgtcc gccactcact tcgcttggta
     1141 atttagtcaa taattgcgcg tttaactcaa aagtagataa atgttgtaat aataattgat
     1201 cctgagcagt attatcagtt aggcctctgt aataatataa cgcttctttt aatgaggtct
     1261 caatcgtcca atcagggtta aagctagtta aagggtgttg gaaaatcggt aacacagcat
     1321 tgtcacttaa gtaaatttct cctttagcag gtttaaacaa gccaagaacc aatgaagcga
     1381 gcgtactttt accacagcca ctttcgccta aaataccaac attttctcca tcaggtatag
     1441 taatattgat atcttgtagc accatctgct ttttattata accaaaagtc acatgtttta
     1501 actcaatcat agtaatccct ctttaattgt gttctatatt taattagacg ttcagtatac
     1561 ggatgcaaat gctcatactt gaaatgatta atattacctc gttcaatgat ttgaccttct
     1621 tttaaaacat aaatgtactg acaatatttc aatacatgac ttaagttatg tgtgataata
     1681 aataatgttt gaccatgttc taatacaata tgctgtaata aatccatcac ttgattaccg
     1741 ttcaaagcat ccaatgatgc aactggttcg tctgcaatga ttaatttagg ctccaacatg
     1801 agaacgcttg ctatgtatac gcgttcaagt tggcccccag aaagttggaa actatattta
     1861 tttaatatat ctttgctttg taaattaacc cacgacaaag ccttatcaac tttggacaaa
     1921 gcctcttctt tactaccttt ataatgctta cgataaatcg cagttaactg tttacctaat
     1981 ttagtatggt cgttaaaact ttctgcataa ttttgagaaa tatagccaat tgtatgacca
     2041 taatattgac tcaatctact aacattttcc ccatcaaatt ggtacgaatc atacgtgcag
     2101 cttaaatcaa atggtaaata ttcaagtaaa gctttagcaa tcaaactttt tccagcgccg
     2161 ctctctccaa tcaaggcatt aatctgttga ctaaaaattt tcaaatcaat ccctttaata
     2221 agagatttct cactagtatt ctttattgtt aaattttgta tatcaatgag actcatcata
     2281 ttcaccccgt tgtttcagca atctatctct tagtgcatca ccggttaaat taaaaattaa
     2341 aatagttata gcaatgactg aagcaggtgc aatcaacata attggatgag acgaaataaa
     2401 atcacgacct tgttgcaaca tagcgcccca ctctggtgtt ggcggttgtg cacctaaccc
     2461 aataaatgat agtgaactta tatatagaat gattttaccg aaatcaacga ccatcaaaac
     2521 gataatagcc ggtataattt taggtgttaa atgacgtatt aatattgttc ttgttggtac
     2581 atgaaataat tgtgccattt ttatataagg cttattcatt tcgctattaa ctatacttct
     2641 agtcaacctt gtgtaattca tccattttat taatgtaatt gagataacta aattccataa
     2701 agatggttga aaaaaacttg ctaaagcaat catgatgata aattctggaa tactcagacc
     2761 aacatcaata aaccttaaca ctaatcgttc aatccaccct tttttatatc cggcaaatag
     2821 acctagtgta acacctatga caacgatagc tattaatgtt aaaacagtaa caaacaatgt
     2881 tgaacgtgca ccgataataa ttcgggtaaa taaatctctc ccataatcat cagttcctaa
     2941 taaatgcaac caactaatag gttcaaaagt ttgtgataaa ttgactttgg ttgcattttc
     3001 actactgaca aagaattgca gtacaattac cacaaaaata aatgcaacga atacaaaaaa
     3061 tatcaggtta ttctttgaaa atattttatg catgacggtc actacttact gatatcaatg
     3121 gtgtattggt tttgattttt ggatttccta attgtaaacg ctgcttcgga tcaagtaata
     3181 acgttaataa atcagcaatc gtattgataa taacaacgaa gaagccaata aataacacgc
     3241 atccttgaat aacaggataa tctcgagatt taatactatc cattaataga taaccaatac
     3301 caggtatatc aaataaattt tcaatcacta cagtaccacc tattagactg ccaagtgaaa
     3361 tccctagtaa tgggataatc ggcaaaattg ttggttttag taaatcatga attaaaatat
     3421 aacgttcatt cataccgcgt aatcttgatg cttgtacgat attactttgc aataacatca
     3481 ataaattaga acgcactaaa cgaatgatgt atgcacacat acctaaagat agcgtgatta
     3541 caggtaatat aaactgactt agtataacgc tatctatatt cattaaattt gtgacaataa
     3601 ataataaaat aataccgata aagaacgctg gtaaactaat cgatagtgtt gagatcactc
     3661 taatcacttt atccgtccac ttatgaaatc gtttggctgc tataatgccg agcggtatag
     3721 atatgcataa cgacactact aatgttgaaa atgatatgag taatgttatg ggtgcatagt
     3781 tgaataatat ctgtgttacc ggttcttttg attcaaaact ttttcctaaa ttaaaatgta
     3841 ataaatgatt catccaatgc caccactgta ccaataaaga atcatttaat cccaatttat
     3901 ctttggttgc atttatttgt tccgtcgaca cttgtgctac atcaagatgt aatattttat
     3961 caacaggatt gcctggtgat aatttcatta aaatgaatgt aagtgtagaa ataacaaata
     4021 aaacaactat catttgcatc agtctataca acatagactt tattatgaac ataatagtcc
     4081 ccctccttgt gtaagttact aacactttct ttttacatga gaatggcgca tgtatatgca
     4141 acttacatat taagaaccaa cgttcattat agtattatcc ataaagaaat tgaagtatat
     4201 ttaatttttt aacaaaatca ttataaaata taatattttg aatcaagtca accatgtaaa
     4261 atataaaaaa gtcaaaacaa aaacaactat agcactgtat tccatctctt tcgaaataat
     4321 tgttactgca gtgtaactta aaagtcgatg attttgtgca tatagttgtc gaatattatt
     4381 ttttatcttt acggcgaagt tcagcgccct catagccgta tttttcaatt tgcttttcta
     4441 atttacgcgc ttttctttct ttacgccaat ttctagtaaa ataccataat agaaaactaa
     4501 ttaataaact cataatcgct aa
//
LOCUS       DAMZLE010000164         4516 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMZLE010000164 DAMZLE010000000
VERSION     DAMZLE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.167"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            228..3101
                     /gene="fnbB"
                     /locus_tag="POB92_002426"
     CDS             228..3101
                     /gene="fnbB"
                     /locus_tag="POB92_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HDI6579890.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATERKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPTTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDTAQPSNVSEIKPRMKRSTDVTAVTEKEVVEEAK
                     ATGTDVTSKVEVEEGSEIVGHNNKETNVVNPHNAERVTLKYKWKFEDGIKPGDYFDFT
                     LSNNVETHGISPLRKVPDIKSKDDNILAVGKVMDERKIRYTFTDYINNKNNLMAELNL
                     NLFIDPTTVTKQGKQTVEVKLGENKISKEFDIKYLDGVKDNWGVTVNGRITLLDKENS
                     KIHHLAYINPKKSDMTSITINGFFAKGGLYTGNVPTVKVYEYLRSDELPESVYANTND
                     QEKFKDVTNDMSDKLTLSENGSYKLTLDALNKKSYVVSFEGKYNENDKELLFRTNLHG
                     YHANYGYYYYYPVSLTWDNGVAFYSNNAQGDGKDKPNDPIIEKSEPIELDIKSEPPVE
                     KHELTGTIEESNDSKPIDFEYHTAVEGVEGHAEGIIETEEDSIHVDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVITESNLVEFDEESTKGILTGAVSDHTTVEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEK
                     DKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTL
                     PQVSGHNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSE
                     PETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKA
                     QSKKSELPETGGEESTNKGILFGGLFSILGFALLRRNKKNHKA"
     gene            3281..4147
                     /gene="galU"
                     /locus_tag="POB92_002427"
     CDS             3281..4147
                     /gene="galU"
                     /locus_tag="POB92_002427"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI6579891.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(4359..>4516)
                     /locus_tag="POB92_002428"
     CDS             complement(4359..>4516)
                     /locus_tag="POB92_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6579892.1"
                     /translation="NRLNNKGMLIKERSLADERIVLIKINKIQYNTIKSIFTDTSKIL
                     KPRKFFF"
BASE COUNT         1784 a          661 c          881 g         1190 t
ORIGIN      
        1 gtaagtataa aaaatacaag cctgttatat caaggtattc aagagctttg tatgcaatat
       61 atatgtgagt ttcaaataat aaaaaactca ttatacataa agttaacaac aatcttaact
      121 ttttattaac tcgctttttt tcattgcttt taaaaaccga acaatataga attgcattta
      181 ttgagttaaa aaataaatga attttacatt taagggagaa tattatagtg aaaagcaatc
      241 ttagatacgg cataagaaaa cacaaattgg gagcggcctc agtattctta ggaacaatga
      301 tcgttgttgg aatgggacaa gaaaaagaag ctgcagcatc ggaacaaaac aatactacag
      361 tagaggaaag tgggagttca gctactgaaa gaaaagcaag cgaaacacaa acaactacaa
      421 ataacgttaa tacaatagat gaaacacaat catacagcgc gacatcaact gagcaaccat
      481 cacaatcaac acaagtaaca acagaagaag caccaacaac tgtgcaagca ccaaaagtag
      541 aaacttcgcg agttgatttg ccatcggaaa aagttgctga taaggaaact acaggaactc
      601 aagttgacac agctcaacca agtaacgtct cagaaattaa accaagaatg aaaagatcaa
      661 ctgatgttac agcagttaca gagaaagaag tagtggaaga agctaaagcg acaggtacag
      721 atgtaacaag taaagtggaa gtagaagaag gtagtgaaat agtagggcat aacaataaag
      781 agacaaatgt tgttaatcct cataatgcag aaagagtaac cttgaaatat aaatggaaat
      841 tcgaggatgg tattaaaccg ggggactact ttgatttcac attaagtaat aatgtagaaa
      901 ctcatggtat ctcgcctctt cgtaaagttc cagatataaa aagtaaagat gataatattt
      961 tggctgttgg caaagtgatg gatgaaagaa aaattagata tacgtttact gattatataa
     1021 ataacaaaaa taatttaatg gctgaattaa acttaaattt gtttattgat ccaactacag
     1081 ttacgaagca aggaaagcaa actgttgagg ttaaattagg tgaaaataag atttcaaaag
     1141 aatttgatat caaatatcta gacggtgtta aagataactg gggagtaact gtaaatggac
     1201 gtataacttt attagataaa gaaaatagta aaatacatca tttagcttat ataaacccta
     1261 aaaaaagcga tatgacctca ataactataa atgggttttt tgctaaaggt ggattgtata
     1321 caggaaatgt tccaacagtg aaagtttatg agtatttgag aagtgatgaa ttaccagaaa
     1381 gcgtctatgc taacacaaat gatcaagaga aatttaaaga tgttaccaat gacatgagtg
     1441 ataaattaac gctaagtgaa aacggtagct ataaattaac tttagatgca ctaaataaga
     1501 aaagttatgt tgtaagtttt gaaggtaaat ataatgaaaa tgacaaggag ttattattca
     1561 gaactaattt gcatggctat catgctaatt acggttatta ctattattat ccagtaagtt
     1621 taacatggga caatggcgtt gcattctact ctaataacgc tcaaggcgac ggtaaagata
     1681 agccaaatga tcctatcatt gagaagagtg aaccaattga attagacatt aaatcagagc
     1741 cgccagtgga gaagcatgaa ttgactggta caatcgaaga aagtaacgat tctaagccaa
     1801 ttgattttga atatcataca gctgttgaag gtgtagaagg tcatgcagaa ggtattattg
     1861 aaactgaaga agattctatt catgtggatt ttgaagaatc tacacatgaa aattcaaaac
     1921 atcacgctga tgttgttgaa tatgaagagg atacaaaccc aggtggtggc caagtaataa
     1981 ctgagtctaa cttagttgaa tttgacgaag agtctacaaa aggtattcta actggcgcag
     2041 tgagcgacca tacaacagtt gaagatacga aagaatatac gactgaaagt aatctgattg
     2101 aactagtaga tgaattacct gaagagcatg gtcaagcaca aggaccaatc gaggaaatta
     2161 ctgaaaacaa tcatcatatt tctcattctg gtttaggaac tgaaaatggt cacgggaatt
     2221 atggtgtgat tgaagaaatc gaagaaaata gccacgttga tattaagagt gaattaggtt
     2281 atgaaggtgg ccaaaatagc ggtaaccagt cattcgagga agacacagaa gaagataaac
     2341 ctaaatatga acaaggtggc aatatcgtag atatcgattt cgatagtgta cctcaaattc
     2401 atggtcaaaa taaaggtgat cagtcattcg aagaagatac agagaaagac aagcctaaat
     2461 atgaacatgg tggtaatatc attgatatcg acttcgacag cgtgccacat attcatggat
     2521 tcaataagca cactgaaatt attgaagaag atacaaacaa agataaacca aattatcaat
     2581 tcggtggaca caatagtgtt gactttgaag aagatacact tccacaagta agtggtcata
     2641 atgaaggtca acaaacgatt gaagaagata caacgccgcc aacaccacca acaccagaag
     2701 taccaagtga gccggaaaca ccaacaccac cgacaccaga agtaccaagt gagccggaaa
     2761 caccaacacc gccaacacca gaggtaccaa gtgagccgga aacaccaaca ccaccaacgc
     2821 cagaggtacc agctgaacct ggtaaaccag taccacctgc caaagaagaa cctaaaaagc
     2881 cttctaaacc agtggaacaa ggtaaggtag taacacctgt cattgaaatc aatgaaaagg
     2941 ttaaagcagt ggcaccaact aaaaaagcac aatctaagaa atctgaacta cctgaaacag
     3001 gtggagaaga atcaacaaac aaaggtatac tgttcggagg attgtttagt attttaggat
     3061 ttgcattatt acgcagaaat aaaaagaatc acaaagcata atcaatctaa aattgacagg
     3121 tttatttcat aaattatatg aagtaagcct gttttttaaa attaaatcaa atttcccaag
     3181 aaataattac atacttaatg acactatgaa ggcgttctaa ttagtgttaa aatgacgttg
     3241 atacatagat ttaatactta ggaaaaggag cacattaact ttgaaaaaaa taaaaaaggc
     3301 aatcattccc gctgctggtt tagggactag atttttacca gcaactaaag cgatgccaaa
     3361 ggaaatgctt cctatcttag ataaacccac aatacaatat atcgttgaag aagctgcaag
     3421 agctggaatt gaagatatta ttatagtgac aggtcgccac aaacgcgcga ttgaagatca
     3481 ttttgatagt caaaaagaat tagaaatggt gttaaaagaa aaaggtaaat ctgagttact
     3541 agaaaaagtt cagtattcaa cagaacttgc gaatattttt tatgtaaggc agaaagaaca
     3601 aaaaggttta gggcatgcga ttagttctgc gcgtcaattt atcggcaatg aaccatttgc
     3661 ggtattatta ggtgacgata ttgttgaatc tgaagtgcca gcagtgaaac agttgattga
     3721 cgtttatgaa gaaacagggc actcagttat aggtgtccaa gaagttccgg aagcagatac
     3781 tcatcgttat gggattattg acccattaac taaaaacgga cgtcagtatg aagtgaaaaa
     3841 gtttgttgaa aagccagcac aaggtacggc accatcaaac ctagctatta tgggacgtta
     3901 tgtattaacg cctgaaattt tcgattattt aaaaacgcaa aaagaaggcg caggtaacga
     3961 aattcagtta acagatgcga ttgaacgtat gaataatgat aaccaagtgt atgcatatga
     4021 cttcgaaggc gaacgttatg acgttggaga aaaattaggc ttcgttaaaa caacgataga
     4081 atatgcattg aaagatgaca gtatgcgaga agaattaaca cgatttatta aagaattagg
     4141 gttataacat taaaaagcat gatactcaat aaaattggaa cacgaaattg tgaactaaat
     4201 tgaatgagtg tcatgcttaa tttatgtgtt gtgatatata gtagggtatt gaaataatat
     4261 aaaaacaaaa gaaagttaat tatgtatttt tatttatact aaattgagaa gtattatgat
     4321 tgcttattta ttttatcaag aggtaaatct gtttaaattt aaaagaaaaa ttttcttggt
     4381 ttgagaatct tggaagtatc tgtgaatatg cttttaatag tgttatattg tattttattt
     4441 attttgatta acacaatcct ttcatctgca agggatcgtt ctttgattag cataccttta
     4501 ttatttaatc tattaa
//
LOCUS       DAMZLE010000165         4492 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMZLE010000165 DAMZLE010000000
VERSION     DAMZLE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.168"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..575
                     /locus_tag="POB92_002429"
     CDS             <1..575
                     /locus_tag="POB92_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6579893.1"
                     /translation="ILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSI
                     VLTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKG
                     NRIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQN
                     GFVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(600..1742)
                     /locus_tag="POB92_002430"
     CDS             complement(600..1742)
                     /locus_tag="POB92_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579894.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQ
                     PGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGA
                     YKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNP
                     LKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQED
                     NKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            1939..2565
                     /locus_tag="POB92_002431"
     CDS             1939..2565
                     /locus_tag="POB92_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDI6579895.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            2926..3711
                     /locus_tag="POB92_002432"
     CDS             2926..3711
                     /locus_tag="POB92_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDI6579896.1"
                     /translation="MKVQIYQLPIIFGDSSKNEAQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPIFKSIKLDLYK"
     gene            complement(4310..4444)
                     /locus_tag="POB92_002433"
     CDS             complement(4310..4444)
                     /locus_tag="POB92_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDI6579897.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTISDLVKWIIDTVNKFTKK
                     "
BASE COUNT         1469 a          671 c          738 g         1614 t
ORIGIN      
        1 ttattttaat taatttaaaa attaaaaatc caacaaaatt agaattagaa gttaaagaac
       61 ctaaaaaata tatcattcca tgggcattgc ttggatttgc attggtaatg atttatcaaa
      121 tggtagtgag cattgtatta acgcaaattt atggtggaca acaagtaagt cctaatacag
      181 aaaagctaat tattattgct cgaaaaatac ctatatttat cttctttgta tctattattg
      241 gtcctttatt agaagaatat gtattcagaa aagtaatctt tggagaatta tttaatgcga
      301 ttaaaggtaa tcgtatcgtg gcatttatta ttgctacaac agtaagttca ttaatatttg
      361 cattagcaca taatgatttc aaatttattc cagtttattt tggcatgggt gtcatttttt
      421 cattagcata tgtttggaca aaacggcttg ctgttccaat tattatccat atgttacaaa
      481 acggatttgt cgttatattc caattactta atcctgaggc tttgaaaaaa gccacggaac
      541 aagcgaattt tatatatcac atttttattc cataataaaa aaaacgaatc acacacggat
      601 tatcgtcgct gtgattcgtt tttatttttc tttttatttc gctttacaaa taagaatagc
      661 atgattgaaa ataccaccat catagaacat acaattgcta gccaactttt actatatgca
      721 gttgacgcac ttttcttatt atcttcttgt tttttaacta ctgtaaaatc gtctttttca
      781 tgcggttcaa atgagcgttg ttttgaatcc ggcttatctt cgggcttttt caaatctggc
      841 attgatccaa tttcaccagt aattaatgcg agtacttgtt catctaaatt acgatagtac
      901 ctttctgatt tcaatggatt gaaatattgg aatctaaagt aattcgattg ctgttgtaat
      961 ccatttaaat atgcatttcc gctgaaattt tgttttcgaa taatattata aatgtcttca
     1021 tcagtaactt ctccataatc tttgcccaac ttattaattt gatttaaaat atacggatta
     1081 tacttgtaag ccccatttgc caaggctaaa aatcgttggg atacaaaatc attaccagta
     1141 ggattttgtg agtttccttg attcccattt ggttgccatt gaccttgatt agatccatct
     1201 gaagcatttg ggttccatgt ccccccattt ttcgagccac cagaatgatt ggaatcccca
     1261 ggttgatcgg gatctggtga cgggttagga tttggcttat tagggtctgg ttttggattc
     1321 ggatttggct ttggtttatc tgggtctggt tttggatccg gttttggttt cgggttatct
     1381 ggatctggtt taggattcgg acttgggttt tggttatttg ggtctggttt tgaatctgta
     1441 cttgggtttt ggttgtttga gtctggcttc acattattcg aatcaggatt agcatcacga
     1501 ctattcgaac ctgttccttc attacccgtt tgatttgatt cgtgaggtgt cccagtctca
     1561 ttattattca tttcactatt tactgatggt gaagttatat tatgattcaa attagtagtt
     1621 ggtttttctc catttaagtt atcctttgct tcaactgtgt gcgttgcaag ccctaaacta
     1681 ctactactta aaattaaaat acttgttgaa agtaacattt tcttaaacca atgatatgac
     1741 atttcacgag acacctccct ttttcatgta agtattaatc taagtataat aaatatgcgt
     1801 tgaaaatgca tttatatttg gaatttgaaa atgaatatta atattcttga aattttcgta
     1861 aatattgctt tacaagtacg agtataaatt ttatacttag tctattaagt attctttact
     1921 aaagaaggag caataattat gggcttattt aaaaaagata aaaaagcaat gacttttgac
     1981 aatgcaatgg aagaaagacg ttcaatatat aatttgaaag attcgatatc aattagcgat
     2041 gacgaattag aatcaatgat tgcacatgca gttaaacatg ttccttcatc atttaattct
     2101 caatcaacta gaattgttct cttgttaaat gacaacaata ataaattttg ggataacaca
     2161 aaagcaattt taaaagaagt catgggagag aatcgtgatt tcgagccaac tgaacaaaaa
     2221 attgataact tcaaacattc atacggtaca attttgtttt atgaagatca agatgttgta
     2281 agtggtttac aagaacaaat gccaaactac tatgataatt ttgcaatatg gtcaactcaa
     2341 acaaatgcca tgcatcaatt tgcaatttgg actgcattag ctactaaagg cataggtgct
     2401 tcattacaac actataatcc acttgttgat gagatgactt caaatgaatt taatattcct
     2461 aaaagttgga aacttattgc tcaaatgcca tttggtgata ttcgtgaagc tgctggcgaa
     2521 aaaacattta atccagtgga agatcgtttc gtaataaaaa aataatcaat atattgttag
     2581 gaatgggaca gaaatgataa agaatcatta gttattcatt atgtagtagt tcttacacat
     2641 tagccgctgc taatgtgcac ttaagaatag gaatacatga gtaaaactca tgtataagaa
     2701 atactaattt ctaaagaaaa agtatttctt tatgttagag cccaccccaa cttgcattgt
     2761 ctgtagaaat tggtgttcca atttctctat gttggggccc catcaactac tgtcaatata
     2821 acattgtaga gcctaggact ttgattttat gtcctgggct cgttttttgc gtttcaacat
     2881 aacttttagt aagttatata taagatctat caaggatgtg atgttatgaa agtccaaatt
     2941 tatcaattac cgattatttt tggagatagt tctaaaaatg aagctcaaat aacgcaatgg
     3001 ttcgaaaaaa atatgaatgc tgaagtagat gtagtcgttt tacctgaaat gtggaataat
     3061 ggctatgatt tagagcattt aaatgaaaaa gcagataata atctaggaca aagtttttca
     3121 tttattaaac atttagctga aaaatataaa gtcgatatcg tagctgggtc agtttctaac
     3181 attagaaact atcaaatatt taatacagct tttagtgtga ataaaagtgg ccagctaatt
     3241 aatgaatacg acaaagttca tttagttcct atgttaagag agcatgaatt tttaactgct
     3301 ggcgaaaacg tagctgaacc ctttcaattg tctgacggta cttatgtgac ccaacttatc
     3361 tgttatgatt tacgttttcc cgaattatta agatatcctg ctcgcagtgg tgctaaaatt
     3421 gcattttatg tggcgcaatg gcctatgtca cgtcttcaac attggcattc attactaaag
     3481 gcacgtgcaa ttgaaaataa tatgtttgtc attggaacta atagcactgg attcgatggt
     3541 aacacagaat atgctggtca ttcgatagtt ataaacccaa atggagattt agttggagaa
     3601 ttaaatgaat ccgcagatat tttgactgta gatttaaact taaatgaagt agaacagcaa
     3661 cgcgaaaata tacctatatt taaaagtatt aaattagatt tgtataaata agaagcgccc
     3721 gaaataataa ttaacacatg tcatgactaa acatagattt atgagagatt tttcataagt
     3781 ctatgtttat ttttttatag tagataactc agtaagaatc catttcgccc tttgcaaatg
     3841 aatgattttc ttaattaaat tgattaattt tttgaccttt tccaacatta gacttattca
     3901 tatattttaa cggtggatct cataatgagg attaactctt cccttcttta ttacaataaa
     3961 aaggccgcga gcttgggagg ggctcacgac catacttatt attaagggaa tgttttacag
     4021 ttattttttc aatctatttt tggggatgtt attaattatg aaaaaaattt tatttatcaa
     4081 ataatgataa atcgatgttg tttacgatag cttacatgct agaaataatt atctgtaatg
     4141 ataattaaga aaaatacata gcactgagtc caaggaaact aactctacta gcaaatgtta
     4201 ctcacttgct aattttacat ggttattaag ttgggatggc ttaataactc atacttttag
     4261 tactatacga agataacaaa tttacaatga aagtaattaa ttattcatct tattttttag
     4321 tgaatttgtt cactgtgtcg ataatccatt ttactaagtc actgattgtt gaaatgatat
     4381 cttgtgccat tgaaatcact ccttccttaa ttaagataaa aattcttaaa attaaacaac
     4441 tcatcaacta ttttccatca catctctgtg atctagttat attaaaacat gc
//
LOCUS       DAMZLE010000166         4485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMZLE010000166 DAMZLE010000000
VERSION     DAMZLE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.169"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..78)
                     /locus_tag="POB92_002434"
     CDS             complement(<1..78)
                     /locus_tag="POB92_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6579898.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIG"
     gene            186..1223
                     /locus_tag="POB92_002435"
     CDS             186..1223
                     /locus_tag="POB92_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6579899.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLAEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLQAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1274..2104
                     /gene="sph"
                     /locus_tag="POB92_002436"
                     /pseudo
     CDS             1274..2104
                     /gene="sph"
                     /locus_tag="POB92_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2342..3358)
                     /gene="lukG"
                     /locus_tag="POB92_002437"
     CDS             complement(2342..3358)
                     /gene="lukG"
                     /locus_tag="POB92_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDI6579900.1"
                     /translation="MIKQLYKNITICTLALSTTFTVLPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDEGKSKF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3380..4432)
                     /gene="lukH"
                     /locus_tag="POB92_002438"
     CDS             complement(3380..4432)
                     /gene="lukH"
                     /locus_tag="POB92_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDI6579901.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKEKRNVTNKDKNSTVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKEGQRLIVTYEVDWKNKT
                     VKVVDKYSDNKSFREG"
BASE COUNT         1515 a          733 c          662 g         1575 t
ORIGIN      
        1 acctatcatt gttagatatt tatatggtat gtaacctaaa actcctgtaa gaagaagcgc
       61 ccctagaaat tgagtcatct tatcacctac tttttatttt attataacat atttagtacc
      121 tagtactaaa ttttgggtag cccgcctacc cttattattt tttgccaatt ttgaggaggg
      181 aacgcatgaa aacacgttgt tacgatggta aaaaatggca atatgaattt aagcatgaag
      241 gaaaaagata ccgtaagaaa ggttttagaa caaagcgtga agctaattct gctggactag
      301 acaagttaaa tgagttaaga agtggtttta atatagataa ctatataact cttgcagaat
      361 acttcgaaaa ttggattaaa acatataaac aacctgttgt taaagaaaat acctaccgtc
      421 attatagaaa cgcattacaa catatacaaa aacataaaat aggtaaaatg gagttatcaa
      481 agataaatag acaagtttat cagaaattca taaacgacta ttcaaaagaa cacgcaaaag
      541 aaactataag aaaaacaaac ggtgctattc ggtcagcttt agatgacgca ttatatgatg
      601 gacttatttt taaaaacccc gcttataaag ttaattataa agccggaaaa cctacgaagt
      661 cagaacaaga aaaattcatc tcggtaactg aatatgaaat actaaaagat cacgtcagaa
      721 agaagagaac tcgttcatca ttagcgctat tcataatgat ttgtacgggt tgtcgtgtca
      781 gtggtgcaag aaatataaag attgagcata tcaaccaagt gaaaaacact atatttattg
      841 acgagcgaaa aaccgatact tcccctagat atatcagtat cgctaaatct gatatgaaac
      901 acattatgga cgtcataagt acatttgcaa ttagctatga tggttacatt ttcaaagaag
      961 ccggatctat aattaacctt caggctatca ataatgcttt gaaatcagcc tgtagagtca
     1021 ataatatacc aattattaca tcgcacgcat taagacacac tcattgttct tatttactag
     1081 caaaaggtgt atctatacat tacatttcta aaagattagg tcataaaaat atagcaataa
     1141 ctacatccgt gtattctcat ttgttagaag aaaaatttaa tgaagaggac aaaaaaacaa
     1201 ctaaaatttt agaaagtatg taatttaggg acccattagg gactccaaac ccaataaata
     1261 ctgttgttac aaggtttcta tgtatccaaa ctgggggcaa tataaacgcg ctgatttaat
     1321 cggacaatct tcttatatta aaaataatga tgtcgtaata ttcaatgaag catttgataa
     1381 tggtgcatca gacaaattat taagtaatgt gaaaaaagaa tatccttatc aaacacctgt
     1441 actcggccgt tctcaatcag ggtgggacaa aactgaaggt agctactcat caactgttgc
     1501 agaagatggt ggcgtagcga ttgtaagtaa atatcctatt aaagaaaaaa tccagcatgt
     1561 tttcaaaagc ggttgtggat tcgataatga tagcaacaaa ggctttgttt atacaaaaat
     1621 agagaaaaat ggtaagaacg ttcacgttat cggtacacat acacaatctg aagattcacg
     1681 ttgtggtgct ggacatgatc gaaaaattag agctgaacaa atgaaagaaa tcagtgactt
     1741 tgttaaaaag aaaaatatcc ctaaagatga aacggtatat ataggtggtg accttaatgt
     1801 taataaaggc actccagagt tcaaagatat gcttaaaaac ttgaatgtaa atgatgttct
     1861 atatgcaggt cataatagca catgggaccc tcaatcaaat tcaattgcga aatataatta
     1921 ccctaatggt aaaccagaac atttagacta tatatttaca gataaagatc ataaacaacc
     1981 aaaacaatta gtcaatgaag ttgtgactga aaaacctaag ccatgggatg tatatgcgtt
     2041 cccatattac tacgtttaca atgatttttc agatcattat ccaatcaaag cctatagtaa
     2101 atagtgctca actaactaat aactcgcttc gttctaaaag gtcgaagcga gttatattgt
     2161 taaaatttga attgacttac attttaataa aatcatctta acaattttaa tttttcatta
     2221 atacaagtct ttactctaca ctcaaacaag attcatacac tgcacatcat aataaatcta
     2281 tctattcaaa tataaataaa agttacctac tacattctat gtagcaggca acttttatta
     2341 cttatttctt ttcattatca ttaagtactt ttacaaactt cacattatgt gtcttccaat
     2401 caacttcata taatgctgat aatttttctt cttttttatc tacatggttt tcaccagacc
     2461 aatagcccca gaaaccatga cgattccaat ctattttaaa ctcatccatt gatcttttat
     2521 aatgaacaac aaattttgat ttaccttcat cttttttatc atgtgacata acagctaaaa
     2581 attctggatt aaacccttca gaaacagtta caggcatctt attcttagga gtaaaattat
     2641 ctttcgccca taaatttcca tttcgtgtta aagaaaagat ttcactttta gttctattat
     2701 cactatcatt agttaattgt ctcgtatggt catgtcccat attatttatc aaatgtgctt
     2761 ctactttcca acctacacct ttatgtgacg tagattgatc aagtaatgta cgatacgatg
     2821 gttgttgata actaatcgtc tctgaataat tactctcttt tgtaatattt ccagttaacc
     2881 ctccacgatt aatcgaaaaa tctccacctg ttttataacc atacgtatat ttaacttctc
     2941 ttgattcatc ctgatttttt ggtgcaaagt cagtcacatt tgtattgttg ttgtcatcaa
     3001 cattttgaat tgaaactgaa taagatccag gccatcttaa tgtactattc caataaccat
     3061 ttgggtctag aattcttaaa ccactaccta ttgttccttt cgctttaata aatactgttt
     3121 ctttatcata attaggttca gttaagaagt taaattgtaa gctttgagta atattttttt
     3181 gactatcact tgttgtagct gtacgtgtat acattttagt atcaccatca agattcttct
     3241 cagaaacagc tttaatttca gaattaattt ttgcataaga agtagctggt aatacagtga
     3301 aagtagtcga tagtgctaac gtacaaattg tgatattttt atatagttgt ttaatcattt
     3361 gtaatccttc ccttcgattt tatccttctc taaatgattt attatcagag tatttatcaa
     3421 cgactttaac tgttttattt ttccaatcaa cttcataagt gacaattaat ctttgacctt
     3481 ctttattttt ttctaaaatt ggaggtgcat aatgtattcc aggtctgttt ttcaaaatat
     3541 cttgatttcg tgtgtatgtt acttcaaatt gcgttttctc atttgacttt tcattagata
     3601 aataagttaa aaattctgga ttaaagccac ttcttactaa tgctgggtat ctatattttg
     3661 aagcaaagct tagttcaggg ttttcaacag tagcaattct cgtatttcta tagaataata
     3721 attcatcatt tctatttttc acttctccac catacttcaa gtcattcgca ataactgacc
     3781 agtgtacatg ccagttatta tttttaccgc tggcaattgt gtcataattt tgctgattat
     3841 aactaatcgt tttggagtag ctatttgatg aagttcgtcc aatacctttc gttgaatcga
     3901 atttaccacc tgagctatat gaaaatgtac tgtctacttt cgcagttgaa attttatttt
     3961 tcggcaattg gtctaatatt tcagttttag gatttctttt tacttgaaaa tctacatggt
     4021 actcacttgg atactttaac caatttgaat ttttttcttc tttatgagat tcaaacttta
     4081 aatttgaatg aattgagcct tgttttttaa caagtaatac attcttgtca taagttggat
     4141 catcgataaa gtcgaattgt aaattttgga gtatatttgt tttctcatca tatacagttt
     4201 cagttcgttt tgtgatttta ccgtttttcc caatatcatc aggtactgtt gaatttttat
     4261 ctttattagt aacattacgt ttctcttttt gttgagactt atcaacatgt tctttcttat
     4321 tttggtcttg cgagtcttta tgagctgaat ttgcttgagt cgttgctgct gataacaata
     4381 aaattgcaca tgataatgat gacgctatta aaacacgttt tttatttttc attgttataa
     4441 ccttctttcg tatgattgat atttgttgat atgtatcgac atgtg
//
LOCUS       DAMZLE010000167         4472 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMZLE010000167 DAMZLE010000000
VERSION     DAMZLE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.170"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            99..782
                     /locus_tag="POB92_002439"
     CDS             99..782
                     /locus_tag="POB92_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6579902.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(898..1560)
                     /locus_tag="POB92_002440"
     CDS             complement(898..1560)
                     /locus_tag="POB92_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI6579903.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(1553..2170)
                     /locus_tag="POB92_002441"
     CDS             complement(1553..2170)
                     /locus_tag="POB92_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI6579904.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2237..2707
                     /locus_tag="POB92_002442"
     CDS             2237..2707
                     /locus_tag="POB92_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDI6579905.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2854..3723
                     /locus_tag="POB92_002443"
     CDS             2854..3723
                     /locus_tag="POB92_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6579906.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            3744..4310
                     /locus_tag="POB92_002444"
     CDS             3744..4310
                     /locus_tag="POB92_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6579907.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         1638 a          633 c          776 g         1425 t
ORIGIN      
        1 atttaaaaag aacaatacta cgagttcatt ttcagggaaa taacgaacat catagaatca
       61 gcataatacg aaataaataa ataggagtat tgatattaat ggaatggata ttatttgata
      121 aagatggcac gttaattgaa tttgatagaa gttgggaaaa aataggggta cgatttgtac
      181 aatcattgct tgagactttc ccagtacata ataaagaagc tgctttaaga caactcggtg
      241 tcattaaaga atctattgat ccaaaatcag tgatgggttc aggatcttta caacaaatta
      301 tccaggcatt taatgatgtg acgggacaag atacaaccga ctggtccaag tcaacaagtc
      361 aaaagctggt agatgaacgt attcctgaaa ttaattgggt agaaggtgtt aaagaagcac
      421 ttatcgattt gaaagcaaaa ggctatcaac ttggtattgt tacgagtgat actaaaaaag
      481 gtgtagaaca atttttagca cataccaatg ctacctcgtt gttcgatttg atcatttcta
      541 ccgaagcgga tgcctatgag aagccaaatc ctaaagtatt atcgccttta tttgagcaat
      601 ataatgtaga tcctcagaaa gtagctatag taggagacac tgctaatgat atgaagacag
      661 caagtaatgc aaatttaggt atggcaatag gtgtattaac aggtattgca acaaaagaag
      721 aattacatga agctgatatt attttaaata gtgcggcaga tattttagaa gctttaaatt
      781 aaaaagaaag acatagtgta tatgtgaatt aatgtattcg atagaattga taatgtaatc
      841 gatgatatct atggaatata cattgaatat aaacatatac gctatgtctt tagtctttta
      901 tcgtgtatct acttgtcgat atgtttgaat aattcgagca attttgtcta tcataggatt
      961 taaagattcg gggtccttat ggatatcata ttcattaata ttgatacgta caactggaca
     1021 tgcattaaag ctattaatcc aatcgtcata gcgtttaaat agctttttcc agtattcagg
     1081 gtctgtatta atttccattt cgcgaccacg ttcaataata cgatcaatga cctcatcata
     1141 gttacattct aaataaatca ttacatcagg tttaggaaaa taaggtgtca tgaccatggc
     1201 attaaataag tctgaatatg ttttgaaatc ttctttactc attgtgcctt cttcttcatg
     1261 catttttgca aaaatatcaa catcttcata aattgatcga tcttggacaa agccaccacc
     1321 atattcaaac atacgctttt gttctttaaa acgttcagct aagaagtaaa tttgcaaatg
     1381 gaaactccat cgttcaaaat cgctgtaaaa tttatctaaa tatggattat gttcgacatt
     1441 ttcaaaagac gttttaaagt ttaatttatc tgcaagtgct tgcgttagtg ttgattttcc
     1501 aacaccaact gtacctgcaa tggttataat ggcattttgt ggaataccgt aattattcat
     1561 tggtaatatc tcctatcata ggtaatataa tatgtaatat atcttcgtaa tcttgttcat
     1621 ttttaagaaa atcaatagaa gttgtatcga ttaaaactac atttgaacca ttactttgta
     1681 aggactcata atactcacga taatcttttt ttaactttaa cagatattca tcttctattt
     1741 gatgctcaaa actacggtta cgtttagcaa ttctagattt taacacatca aggtctgcat
     1801 ctaaaaagat aatcatattc ggcataatca tatcttcagt taaaatatca taaattttac
     1861 tgaatttctg aaattcaaca gaactcaaag tatttttagc aaatatctta tttttatgta
     1921 tatgataatc actaactaca ccttgattta gttgtgttac atcttgaaat tgcttatatc
     1981 tattgcataa aaagaacatt tcagtttgaa aactccattt agagatatct tcataaaagt
     2041 ctgataaaaa tggattttct gtgatgattt ctttttcttc ataaaaatct aaagtttgac
     2101 ttaatttgtg tgcaagtgaa gatttaccta cgccaatagg accttcaatt gctataaaag
     2161 gtttgttcat attcacatct ccaaatgtta aaatagacat tgagtattgt accataagtg
     2221 aggtatctgg acataaatga caaatgatat atattttatg acattagcga ttgaagaagc
     2281 taaaaaagca gctcaactag gcgaagtacc tataggtgct atcatcacta aagatgatga
     2341 agttatcgct agagcacata atttaagaga aacactacaa caaccaacgg cgcatgctga
     2401 acatattgca attgaacgtg cagccaaagt gttaggtagt tggcgtttag aaggttgcac
     2461 attatatgta accttagaac catgtgtcat gtgcgcagga acaattgtaa tgagtcgcat
     2521 tccaagagtc gtctatggcg cagatgatcc taaaggtggt tgtagtggca gtttaatgaa
     2581 tttattgcaa caatctaatt ttaatcatcg tgcaattgtt gataaaggtg tacttaaaga
     2641 agcatgtagc acattattaa caacattttt taaaaactta agagccaata agaaatccac
     2701 caattagacg atagtacata ctaattaaaa tttgttagaa ttacattata tgataaatta
     2761 atgacaatat aaatgttaat agaaaagtat gtgcgctgag tatatattct tatttaagaa
     2821 caagatttta aatttacgaa acgaggtaac ataatgataa aactaatagc cactgatatg
     2881 gatggcacgc tacttaatgc agcacatgaa atttctcaac ctaatattga tgcgattaaa
     2941 tacgctcaag aacaagggat aacggttgtt atcgcgacag gtcgagcatt ttatgaagca
     3001 caagcaccag ttgctgacac agatttaaca gtaccatata tttgtttgaa tggtgctgaa
     3061 gtacgtgatg aaactttcaa tgtaatgagc acttcacacc ttaataaatc gctagtacac
     3121 aaaattacaa atgttttaaa agatgcaggt atttattatc aagtatacac gagtcgtgcg
     3181 atttatactg aagatccaca aagagattta gacatttaca tagatattgc tgagcgtgca
     3241 ggtcaacatg caaacgttga gcgtattaaa aatggtattc aaagacgcat agataatggt
     3301 acgttgaaag ttgttgataa ttatgatgct attgaaaaca tacctggtga attaattatg
     3361 aaaatattag catttgatgg aaatttagaa aaaattgaca aagctagtaa aattttagct
     3421 gaatctccga atttagctat atcatcatct tcgagaggaa atatagaaat aacgcattca
     3481 gatgcacaaa aaggtattgc gctagaaaca attgccgaaa gattagggat tgaaatgaaa
     3541 gaagtcatgg caataggtga caatttaaat gacttatcaa tgttagagaa agttggctat
     3601 ccagttgcga tggaaaatgg tgcagaagaa gttaaaaaaa tagcgaaata tgtcacagat
     3661 acgaatgaaa atagtggtgt tggaaaagct attatgaaat tattacgtga acaacaagtt
     3721 taataaaaaa agaggggtca aatatgaaag gattaattat tattggcagt gcacaagtga
     3781 attcacatac aagtgcacta gcaagatact taactgagca ttttaaaaca catgatattg
     3841 aagcggaaat attcgattta gcagaaaaac cgttaaatca attagatttt tcaggaacaa
     3901 caccgtctat tgatgaaatc aaacaaaata tgaaagattt aaaagagaaa gcaatggcgg
     3961 cggacttttt aatattagga acgccaaact atcatggttc atattctgga atattgaaaa
     4021 atgcattaga tcatctaaat atggattatt ttaaaatgaa acctgtaggc ttaataggaa
     4081 atagtggtgg tattgttagt tcagagccat tgtcacattt aagagtaatc gtcagaagtt
     4141 tactaggcat tgctgtacca actcaaatag caacacatga ttctgatttt gctaaaaatg
     4201 aagatggttc atattactta aatgatagtg aattccaatt acgagcaaga ttatttgtcg
     4261 atcaaattgt atcttttgtg aataatagtc catatgaaca tttaaaataa tattaaaaaa
     4321 tatgtaaata tacattaaaa ataggtatgt agcttaaggt atttcattga gaatgctttt
     4381 aagtcacatg cctatttttt gttctaatgc accaatagta agcctttgat attactgttg
     4441 tgagcaatgt tattaattaa aataagatgt aa
//
LOCUS       DAMZLE010000168         4467 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMZLE010000168 DAMZLE010000000
VERSION     DAMZLE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.171"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(1420..2355)
                     /locus_tag="POB92_002445"
     CDS             complement(1420..2355)
                     /locus_tag="POB92_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDI6579908.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALL
                     KDRFELSIQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELISFNLLTQERVG
                     LNKPNRLYLLKPNYDIKASHSKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTENN
                     DTDYIKTESNDTNDLNDNKLTFPSNHTNHSNHDNSNFNNEALKFQLLEELPQSIKNYL
                     SNFEVAEIKIIKTVLLKAKTSFNNAIDSYYLLEDMEIEILHVLKRFKAMLIQKNETVE
                     SMQGYLMKSLKSEFAEMHTLNKRRDHLPITSLFNQ"
     gene            2795..3562
                     /locus_tag="POB92_002446"
     CDS             2795..3562
                     /locus_tag="POB92_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDI6579909.1"
                     /translation="MRTIKMVADELGVTKQTIVNNAKSLNITFEKENGINYIDDNDYL
                     KIIEKITKKESTIQNKESVKKELFGENTAKAEKNIYNNSNSSETLKTKVNELEKQIEI
                     FETRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLESQLEEER
                     QLNYSFDTATNNRQNINPQEATFTEESQNINQQQTKRQPTEVNYKDIAEEKKNDSTDE
                     GELTKEDKFVEETKSDPDDNREVEPPKKGFWSRFFGN"
     gene            complement(3687..3809)
                     /locus_tag="POB92_002447"
     CDS             complement(3687..3809)
                     /locus_tag="POB92_002447"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDI6579910.1"
                     /translation="MAPYTNKGYAVKELCSYLQINMDDVYTIGDRKLIYLCLIP"
     gene            complement(3885..4445)
                     /locus_tag="POB92_002448"
     CDS             complement(3885..4445)
                     /locus_tag="POB92_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579911.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLC
                     LTLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRV
                     IKKYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMI
                     MIFDLIIGLLLNFQIFLKNKKYDDFL"
BASE COUNT         1690 a          549 c          622 g         1606 t
ORIGIN      
        1 ttttatattt tttaattctc tagttaataa aaagataaaa tgatctttat ttcgattcac
       61 tttttaataa aattgagcat agaaacgtat agaaattgtt ttattctttc tctttttttg
      121 aacttaaaaa taaataatat gatcattatt gaatttgata ttgagaaaaa agtatttaat
      181 caatttcaaa aagtgaaaca aatgtatgaa ttgcttagat tactttttta gtgattttgt
      241 tgttgaaatt aatactaaaa gtagtaggat taataggatt tggttaaaaa aattaatcct
      301 atttatagga ttgaggatga ctgaagccta tgaaatcaac aaaaataata gtattcaaac
      361 aatttaaagt ggtcttgtaa caattgtaaa tagtatgcaa tattaaggtt tataatgtcg
      421 attaattttt atttaatatt aactataaat gttgttatat caacacttgt agtgaatttc
      481 aaagagcaag taagcaaaac gaacggtaag acaatccttc agctatgctg aataccacca
      541 aaggtggtac tggatttcct tatgctcatg tcaaatacta tttgaaaatc ctattatgtt
      601 tgtaatattt ccaaaaacaa aacgtgcttt aagacaaaag tggctattct cttgttcact
      661 ttttacttta agtgcttatt tttattttca aaataagcat tgttagtaaa ataattggag
      721 ataaaaaaag agaaaactaa gtaaaaatag ttacttagtt tgggatagaa aaaatcaaat
      781 ttaaaattac attcgaccca tgttttgaac attaacgact tattttttgt ttatgatgta
      841 tatcaattga atctgaattt tgattttatg gaatgataaa ttcaactttt tctttccgat
      901 cattaacaaa ataaatgaca aaaacgattt tattaagtca cttttataat taagaaagat
      961 tgctaaagaa tataaaaacg gttagtgttt ttatattctt taaagttaaa gcaacgttaa
     1021 ataatcattt tatttttcat cttatgcgac gtttttgagc cattttcaaa cgaataataa
     1081 ccgtttatag aaaaaaatag gactaaaaca gaaacgtttt agtcctacct acttgcttaa
     1141 ctacagttca ttttcagatt tactcaattg ccattgaaga taaagttcga ctgcagttaa
     1201 aagaacatta atcattgcca aagtaatttt aatctttttc ttagtactca taagtcttcc
     1261 tcctttcttt aatagcagca tgctatgttg tgaaattgta ttgatgttat tttgttatct
     1321 cctcccttac ctttcgtttg atctaggtgg ttgctttagc aaccacctgt taataaaaaa
     1381 aagagaacat ctatttaaag atgctctcta aatcaatcat tattgattaa ataaagaagt
     1441 gatgggtaaa tgatcacgtc gtttattaag cgtatgcatt tcagcgaatt cagacttaag
     1501 agatttcatt aagtatcctt gcattgattc aacagtttca tttttttgaa taagcatagc
     1561 tttgaaacgt ttaagaacat gaagtatttc tatttccata tcttctaaca agtagtagct
     1621 atcaatcgca ttattgaaag atgttttggc ttttaataat acagttttga taattttaat
     1681 ttcagctact tcaaagttac ttagatagtt tttaatactt tgtggtaatt cttcgagtaa
     1741 ttggaatttt aaagcttcat tattaaagtt tgaattgtcg tgatttgaat gatttgtatg
     1801 attactagga aaagttagtt tattatcatt caaatcattc gtatcattac tctcagtttt
     1861 aatataatca gtatcattat tctcagtatc attagactgt gaatttggaa gttcttgacc
     1921 acttaaattc acagttctag aacttccaaa ttcattgttc tggaactgtg aatttggaag
     1981 ttccttgcta tgactggctt taatgtcata attaggtttt aataaatata atctatttgg
     2041 tttattaagg ccaacacgtt cttgagttag taaattaaaa cttattaact ctttttttaa
     2101 ttttataatt ttttgttctg cataacctaa gtattcgcac aaatgtttat tactgaaaat
     2161 aaagtaaatg ttattatttt tatctaccca ttcattttgg atggataatt caaagcgatc
     2221 ttttaataac gcatacataa ttttggcttc cgccgacatt tttttaaaat atgcatcttc
     2281 gaataaatat ttaggtagtt gataaaacct ttcagttttt acttgtgata aattgtattt
     2341 ttcaaaattg ggcataataa aaacctcatt tccattttta ggattgaggt aataaaacaa
     2401 tttttacgta agttatatgt tttgtgcttg cacttagatg attagataga tataatacaa
     2461 gtatcaaaaa atatataaac aaacgagtgc gaatcgtttt attaccgaga gtcaatgcta
     2521 cgccaatagc attgactctt ttagtattta taaagcaata ttaacacttt taagaaagta
     2581 tataaagagt tttaaatgta gttaaaagca cattgccatg gcaaaatgtg tagttcattg
     2641 ttttagtacc taattaagct tcaaaaacat attataggta gttaattagg tgcatatgta
     2701 gtaaataggt agtaaattta gtggtaattg atgaaataaa ctgcattagg tggtaaaatt
     2761 taggtactaa attaggtacc taagggtgga tgttatgaga actattaaaa tggttgctga
     2821 tgaattaggt gtaactaaac aaactattgt taataatgct aaaagtttaa atataacttt
     2881 tgaaaaagaa aatgggatta attatattga tgataatgat tatttgaaaa ttatagaaaa
     2941 aattactaag aaagagagta caatccaaaa taaagaatca gtaaaaaaag aattgtttgg
     3001 tgaaaatact gcaaaagcag aaaagaatat atataataat tcaaatagtt ctgagacatt
     3061 aaaaacaaaa gtaaacgaat tagaaaagca aatagaaatc tttgaaacta gagctaaaaa
     3121 tgatgaaaaa tatattgaga atttaactaa gcaattagat caacaaaata gcaatgttaa
     3181 tacattaaat aaattgttgg agaatcaaca aatattagct ttagaaagca ataaaaaaat
     3241 tcaaaaacta gaaagtcaat tagaagaaga aagacagctt aattattctt ttgatacagc
     3301 gacgaataat agacaaaata ttaatccgca agaagcaact tttactgaag aatcgcaaaa
     3361 tatcaaccaa caacaaacaa agcgtcaacc aacagaagta aattacaagg atatagccga
     3421 agagaaaaag aatgacagta cagatgaagg tgaattaaca aaagaggata agttcgttga
     3481 agaaacgaag agtgatcctg atgataatag ggaggtagaa ccacctaaaa agggattttg
     3541 gagtcgattc ttcggtaatt aaaaatgaat gtaaaaggtg tatcaaggga gtataaatct
     3601 ttgatacacc ttttatagtg atgaaacttc tgataatcat cagttatgtt aaactctaac
     3661 aaaaactaaa ttatcagaag aattatctag ggtattaaac ataggtaaat caattttcta
     3721 tcgccaatag tataaacatc atccatatta atctgaagat agctacataa ttctttaaca
     3781 gcatagcctt tatttgtata aggcgccata aactctatta aatatggttt gttttgaggt
     3841 ttctatgttt aatcatttaa tttttcatta tataaatcga ataattataa aaagtcatca
     3901 tactttttat tttttaaaaa tatttgaaaa tttaatagta atccaattat aagatcaaat
     3961 atcattatca ttataatcaa ggcaactata atcactgtga cactcgttac ataacaagct
     4021 agtaatctcc agataaaatt gacattttca ccaattccat aacttaacat attacaaagt
     4081 agtaaaagaa taataatacc tatactgtaa attaaaattg tcagtgtata ttttttaata
     4141 actctattag gaaacaatag ttcatctatt aggttctttt tcaccagtat ctctcgttca
     4201 attctatctt tgtcacagat tttgatttta ctatttttta ttttttcctc taataaaata
     4261 acctcgaacg atctgctaat ttttaataaa tatgcaggaa aagctaatgt taagcataat
     4321 gtaattaatt catttacatt gttcatgtaa cttataaaat agatgaaagt tgctactagt
     4381 gctcctaaaa atatagttat attaagttct acatcaaata ttttaaaaat atctgctcta
     4441 ttcatcagtt aatcatcctt tcaaggt
//
LOCUS       DAMZLE010000169         4431 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMZLE010000169 DAMZLE010000000
VERSION     DAMZLE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.172"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            132..731
                     /locus_tag="POB92_002449"
     CDS             132..731
                     /locus_tag="POB92_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI6579912.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            852..2927
                     /locus_tag="POB92_002450"
     CDS             852..2927
                     /locus_tag="POB92_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDI6579913.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIRKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            3040..3189
                     /gene="rpmG"
                     /locus_tag="POB92_002451"
     CDS             3040..3189
                     /gene="rpmG"
                     /locus_tag="POB92_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6579914.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            3398..3937
                     /locus_tag="POB92_002452"
     CDS             3398..3937
                     /locus_tag="POB92_002452"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI6579915.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3949..>4431
                     /locus_tag="POB92_002453"
     CDS             3949..>4431
                     /locus_tag="POB92_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI6579916.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     Y"
BASE COUNT         1737 a          605 c          791 g         1298 t
ORIGIN      
        1 attatttttg atttgagtta tattaaatga gtagagaaaa taataagtag tagaatttaa
       61 gcaaattctt tgttgaacca gtcactgctt gttattattt acttacagac attttaggag
      121 gatgattatt tatggctttt gaattaccaa aattaccata cgcatttgat gcattagaac
      181 cacattttga caaagaaact atggaaattc atcatgacag acatcataac acttatgtta
      241 cgaaattaaa tgctgcagta gaaggtacag atttagaatc taaatctatt gaagaaattg
      301 ttgctaattt agacagtgta ccagctaaca tccaaactgc tgtacgtaat aatggcggtg
      361 gacatttaaa ccattcatta ttctgggagt tactttcacc aaactcagaa gaaaaaggta
      421 ctgtagtaga aaaaattaaa gaacaatggg gttctttaga agaatttaaa aaagaatttg
      481 ctgacaaagc agctgcacgc tttggttcag gttgggcttg gttagtcgta aacaatggcc
      541 agttagaaat tgtgactaca ccaaaccaag ataatccatt aactgagggt aaaacaccta
      601 ttttaggttt agacgtatgg gaacacgctt attacctaaa atatcaaaac aaacgccctg
      661 actacattgg cgcattttgg aatgtagtta actgggaaaa agttgacgaa ttatataatg
      721 caacaaaata aagtatgatt acttgttcgg tctcttaaat gagaccgtta tttttttgtc
      781 aaaaagatag aaataatttc taaattcata tatgatttaa agtgaaagac tttgaataga
      841 ggtaggtagt tttgttaaaa agactaaaag aaaaatcaaa tgatgaaatc gttcaaaata
      901 caattaacaa gagaattaac tttatatttg gtgtgattgt atttattttt gcagtactag
      961 tactacgttt aggttattta caaatcgcac aaggctcaca ttataaacaa attataaaaa
     1021 atgatgaaaa cattacagtg aatgagtctg tgccaagagg tcgtatttta gacagaaatg
     1081 ggaaagtttt agttgataat gcttctaaaa tggctattac atatactagg ggtcgaaaaa
     1141 caacacaatc ggaaatgttg gatacggctg aaaagttatc aaagctaatc aagatggata
     1201 ctaagaaaat tacagaacgt gataagaaag atttctggat tcagttgcat cctaaaaaag
     1261 caaaagcaat gatgacaaaa gaacaagcta tgttagcaga tggaagtatt aaacaagatc
     1321 aatatgataa acaactgtta tcgaaaatca gaaaatcaca attagatgaa ttgtcttcta
     1381 aagatttaca agttttagct atttttcgag agatgaatgc aggaacagtt ttagatccac
     1441 aaatgataaa aaatgaagat gtcagtgaaa aagagtatgc agcagtttct cagcaacttt
     1501 ccaaattacc aggtgttaac acgtctatgg attgggatag aaaatatcca tatggcgata
     1561 ctttaagagg tatattcgga gatgtatcga cacctgctga aggtattcca aaagaattga
     1621 cagaacatta cttatccaaa ggatattcac gcaatgatcg tgttggaaaa tcttacctag
     1681 aatatcaata tgaagatgta ttgcgtggta agaagaaaga aatgaaatac acaacggaca
     1741 aatctggtaa agttacatct tcagaagtgt taaatcctgg cgctcgcggt caagatttga
     1801 aattaacgat cgatatagat cttcaaaaag aagtagaagc attattagat aaacaaatta
     1861 agaagcttcg cagtcaaggt gccaaagata tggataatgc aatgatggtt gtacaaaatc
     1921 ctaaaaatgg agacattctt gcgcttgccg gaaagcagat taataagagt ggtaaaatga
     1981 ctgattatga cattggtacg tttacttctc aatttgcggt tggatcttct gtaaaaggtg
     2041 gaacattatt agctggttat cagaataaag ctatcaaagt tggagaaaca atggtcgatg
     2101 aaccattaca tttccaaggt ggtttgacaa aacgatcata cttcaataaa aacgggcatg
     2161 tatctattaa tgataagcaa gctttgatgc attcatcaaa cgtatatatg tttaaaacag
     2221 cattaaaatt agcgggagac ccttattatt ctggtatggc tttaccttca gacataagtt
     2281 cacctgccca aaagctaaga agaggattaa atcaagtagg cttaggtgtg aaaacaggga
     2341 tagatttacc aaatgaaaca agaggtcaaa tcgaaccatt aacaaataat ccaggtaatt
     2401 atctagattt atcaattggt caatatgata cctatacacc attacaatta tcacaatatg
     2461 tttcaactat agcgaatgat ggttatagaa tacagccaca cattggatta acgattcatg
     2521 aatcaactaa taaagatgag gttggtccac tcaagaagaa aattaatggc actgtcttga
     2581 acaaggttaa taatactgaa aaggaaatca aacaaattca agaaggattc aaaatggcat
     2641 ttaatgataa agatggtact ggatatgtta gttttaaaga tacagtagta cctactgctg
     2701 gtaaaacggg taccgctgaa gtgttccaaa acggagagcc aagagttaac tctacttata
     2761 taggatacgc gccaattgat gatccaaaat tagcgttttc aattgtatat acaaatcagc
     2821 ctgtaccacc accatggtta acaggtggag acttaggtag agatgtaatt aactactact
     2881 ttaagcagtt aggtaaagat gataaaaata aagacaaaga caaataaaat ttaacctgac
     2941 gattgtgtag cgcatggttg taaaatttta actttgcaaa tattatagat gttggtataa
     3001 taataaagtc gtatttagaa atgataagga ggaataacca tgcgcgtaaa cgtaacttta
     3061 gcttgtacgg aatgtggtga cagaaactac attacaacta agaacaaaag aaataatcca
     3121 gaacgtgttg aaatgaagaa attctgttca cgtgaaaaca aacaaacttt acaccgtgaa
     3181 acaaaataat cattaaaatt aaatttaaat taacaataaa aaaggtgaaa tctcaaaata
     3241 agaggtttca cctttttaat tatgcacacg tatgaaatga taaaagattt tcaattgaaa
     3301 taataaattt tagaattgtc gcataattca tcatgacaac ataatgacat gtattgttat
     3361 taacgatata atagaattga ataaagtggg tgataatgtg actaaaaatg agattagaaa
     3421 atacatttta cataaaatga agaattttaa taaagctgaa aagcgaaaag cagacacatg
     3481 gttaagaaat caattttttg caactgaaga atacaaagaa gcaaacgcaa ttgcgctagt
     3541 tctttctttt aatcatgaag tagatacttt ttctattatt gaacaagcct taatggatca
     3601 taaacgtatt tttgtaccga aaatggatta tttaaatcat caaatgactt ttaaagaaat
     3661 atttaatctc aaagatattg atgtcgataa taaggggatt tactatccaa cttcaaaagg
     3721 tgaaacaacg aataacctag atttaattgt tgttcctggt gttggatttc aagacgatgg
     3781 atatagaatt gggtatggtg gtggctatta cgacaggttt ttagctaatt atcagacaaa
     3841 gacaataagc ttattatacg attttcaaat aacatcattt gaaccggaat cattcgatca
     3901 accagtcgat aaattgatta tatatcaatc agcatagtgg aggactaaat gaacatagac
     3961 aaacaatttt ggaaaacaat atattattgg attaggtact tgaattttga tattgtcagt
     4021 agggaaaaag atgaccaaga aatttggtta gcccataaaa gaaaaaaaca agtagtcatt
     4081 tttaaacaac atatcaagtc tacccaagaa atacgttttg acaaagcgaa agtgcttgaa
     4141 cacaaagatg aaatagcaaa ttttatttct ttcgaaccac aaagttttga attttattat
     4201 tttacagaat cggaattttc agaagaacaa ttaaatgaag tttcgccaat tagaattaaa
     4261 ttcaatgtta taagacacac aaaagatttg ataaagcata tgccgaatat atttttggct
     4321 agacttattt cagaagataa tgataaaaag acatatatgt tctataaacg caaagtatta
     4381 accgataact ttttagataa atatatgcag aaattttcac cggcaacata c
//
LOCUS       DAMZLE010000170         4424 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMZLE010000170 DAMZLE010000000
VERSION     DAMZLE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.173"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(143..1951)
                     /locus_tag="POB92_002454"
     CDS             complement(143..1951)
                     /locus_tag="POB92_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDI6579917.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLDGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCRNFDDVAQKINAMIFS"
     gene            2083..2475
                     /locus_tag="POB92_002455"
     CDS             2083..2475
                     /locus_tag="POB92_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDI6579918.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2477..>4424
                     /locus_tag="POB92_002456"
     CDS             2477..>4424
                     /locus_tag="POB92_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDI6579919.1"
                     /translation="MSRLLNDFNQSLKKGFIDKDISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFELLWQKSTPLTQQWIKSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSI"
BASE COUNT         1622 a          606 c          752 g         1444 t
ORIGIN      
        1 catgaaaatt aattttctaa tatacttttg aaaaattatt acaaattagc cccttcaaaa
       61 cgcgaaaaca taaggattct agtttcaaaa gggctgataa gtataaaatg aaatgtaata
      121 tttcgatgta taaaattttt aattagctaa aaatcatcgc attaattttt tgagctacat
      181 catcaaaatt ccgacatttt aacgacacat ataatttaat tttaggttca gtaccagaag
      241 gacgtaaagc gataaatcct tcgtcaaata agacacgaat aacatttgat ttaggagaat
      301 taatctgtga cgttgtatct ttatccaaat gataaacctc gctagttaaa taatcttcaa
      361 ttgctttcac tttcagtcct tgaatctctt gcggtggatt tgaacggaaa tgtgtcataa
      421 ttgattcaat ttttttctta ccttctaatc catctagcgt atgcgagaat aatgtatcct
      481 catgtctacc aacagtttga taaatctgtt ctaattcatc tttcaatgtt ttgccatata
      541 attttaactc agaagcgtat tttataatga gtggcacaat ttgtacggca tctttatcac
      601 gtacaaaagg ctctgataga aaaccgtaac tctcttcaaa ggcgaaaatc atattttgat
      661 gatcgtccag ttgtcttatt tcctgagcaa taaatttaaa gcccgtaagc acctctttgt
      721 attcaacatt attatagcgt gctaatgact ttgttaactc actacttaca atagattgaa
      781 tcattaagcg atgacgtaat tgagacgttt gttgaatacg ataattaagt aacaacgcac
      841 caatttggtt gccattaaaa tatgtgatat gaccatgtgc atcacgttca gcaatgccaa
      901 gacgatccgc atcagggtca gttgatatta ataagtctgc atgactttta tttgcaagtt
      961 cgactgcttg atcaaaagcg cgatgatctt caggattagc actttgaact gagctgaaat
     1021 tcggatcagg tttacattgt gcttctacaa gattaaattg attgaaattc aaagattgta
     1081 ataactcagg tacaatcgga acacttgtac catgtaaact agtgaacaca acttgtaaat
     1141 cagatttagg aatatagcca atcatatttt gaatgtgttt catatagtca tcagttacag
     1201 atttcggaaa aggcttgata taagatgtat tttgtttaga aataggtata tcaatctgta
     1261 atggatcgcc aacttcttcg atataacgac ttgcaagctc agatgcatca gtcgataatt
     1321 gcgcaccatc agaaccatat actttgatgc cgttatagtc tttcggatta tgacttgctg
     1381 taatcataat gccagcagta gtattaagat tgcgcaccgc gaacgataat tccggtgtcg
     1441 ttttatatgt atcaggtaaa taaactgtta tttgatggtt tgctaataca ttagcaatta
     1501 tttgggcgaa ttcagttgaa agatgtctaa tatcataatg aatgactatt gttggactgt
     1561 ttgtttgggc atttaaataa cgcgctaaac ctaatgccaa tttttcaata gtaaacttat
     1621 taagtcgacc ttcaccaaga ccgaattttc cgcgtatacc cgcagtacca aatgttaatt
     1681 tactttcaaa accttctcgc tgttcaatgt cagattgctg ctcataaaaa tgtttaacta
     1741 aactatcatt agctcgttct atccataatt ctttatccat tgttgctaaa catcctttca
     1801 aaatctcagt tagacttaat aaaacatgaa aactaaagcc cttacattta tgtaatgaat
     1861 tataaagaaa tacgccccaa aagtaaaaaa acacagcccc aagacaatac ttttcacaag
     1921 tattatataa tagatgtgta tgaaaatgca tggagtagat gtaagagtga tatatcatgc
     1981 aggaaattgt aaaatattaa tttttaatat tgatttatat atagttttgt aatttttgat
     2041 agaataacaa ttaagatgat tttaaattga agggtgagtc ttatgaaaaa agtaatcaat
     2101 gtagtaggag ctattatttt ttctgataac aaaattcttt gtgcacagag aagtgaagaa
     2161 atgagtctac ctttaatgtg ggaatttcct ggcggtaaga ttgaaaagaa tgaaactgaa
     2221 aaagaagctt tgatcagaga aattagagaa gaaatgaaat gtgatctaat tgttggagac
     2281 aaagtcataa ctacagaaca tgaatatgat tttggcattg ttaagttaac aacatacaaa
     2341 tgtactttaa acaaagagtt accaacttta actgaacata agagtattga atggttgtca
     2401 ataaacgaac ttgataaatt aaattgggcc ccagcggata taccagccgt taataaaatt
     2461 atgactgagg gataatatga gtagattact aaatgatttc aatcaatctt taaaaaaagg
     2521 ttttatagac aaagatattt ctcataaagg gaattataca ccgaagcttt tagtaaataa
     2581 taaaaatgaa aaagtccttt caacaattat agatgaactt caaaaatgtg aaacgttcta
     2641 tttttctgta gcttttataa cagaaagcgg attagccagc ttaaaggctc aactattaga
     2701 tttaagcaat aagggtgtta aagggaaaat attaacatct aattacttag gatttaatag
     2761 tcctaaaatg tatggagaat tacttaaatt aaaaaatgta gaggtaagat taactgatat
     2821 tgctggattc catgccaaag gatatatttt tgaacataag gattatagtt ctatggtaat
     2881 aggaagctct aatttaacat ctaatgcgtt aaaggttaat tatgagcata atgttttact
     2941 gtctactatg aaaaatggcg atttagttga tagtgtaaaa aatgaatttg aattgttatg
     3001 gcaaaagagt accccactga ctcagcaatg gattaaatca tataaagaat catttgagta
     3061 ccgctcctta gaaaaattag cggaggttga acaaactcaa atgctgctag ctgataaggt
     3121 gaaaaaatca gtggaaattg tacccaattt aatgcaagca gaagcattaa ggtcattaaa
     3181 agctattagg gataaagcca aagataaggc gttaataata tctgcaactg gtacaggtaa
     3241 aacgatttta tgtgcattag atgttagaga agtaaaccct aataaatttt tatttattgt
     3301 tcataatgag gggattttaa atagagctaa ggaagaattt aaaaaagtat tacctataaa
     3361 aaatgatagt gattttggat tgttgacagg aaaacataga gatgtcgatg ccaaatattt
     3421 atttgcaacc attcaaacat tatctagaga tgataatttt aagcaatttg atgaaaatga
     3481 atttgattat attgtttttg atgaagcaca tcgttctgct gcatctactt atcaacgagt
     3541 atttaactac ttcaaaccta agttcatgct gggaatgact gcaacaccag aaagatcaga
     3601 tgaattaagt atatttgaac tgtttgatta taatattgcg tatgaaataa ggttacaagc
     3661 agcattagaa agtgatattt tatgtccatt tcattatttt ggtgtgactg attatgtaca
     3721 tcaaggtatt aaagaagatg atgtaactaa actgagatat ttaacttctg atgaaagagt
     3781 taattatatt attcaaaaga cagattacta tggatattca ggtgaaattt tacaaggatt
     3841 aatttttgta agtagcaaaa aagaagctta tgatttagcg gataaactaa gttcaaaagg
     3901 gattaaaagt gttgctttaa caggagatga tagtgttaat tatcgccaaa ttgtaataga
     3961 aaaattaaaa gaaggtaaga ttaactatat tataactgta gatcttttta acgaaggtat
     4021 tgatattcca gaagtcaatc aagttgtaat gttgagacca acagagtcta gtattatatt
     4081 cattcagcaa cttggtagag gattaagaaa aagttcaaat aaagagtatg ttacagtaat
     4141 tgattttatt ggtaattata agacaaatta tttaattccg attgcgcttt ctggggatca
     4201 atcgcaaaat aaagataatt ataagaagtt tttaacgaat aacgattcga ttaatggagt
     4261 atctacaatt aattttgaag aagttgctaa aaaacagatt tacaattcat tggatgcagt
     4321 atcattaaat caaaataaat taatattaaa agcttatgaa gaagttgaaa atagattggg
     4381 acacatgccg ttactaatgg atttcataca acaacattct atag
//
LOCUS       DAMZLE010000171         4399 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMZLE010000171 DAMZLE010000000
VERSION     DAMZLE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.174"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..132)
                     /locus_tag="POB92_002457"
     CDS             complement(<1..132)
                     /locus_tag="POB92_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6579920.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     "
     gene            307..591
                     /gene="groES"
                     /locus_tag="POB92_002458"
     CDS             307..591
                     /gene="groES"
                     /locus_tag="POB92_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6579921.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            667..2283
                     /gene="groL"
                     /locus_tag="POB92_002459"
     CDS             667..2283
                     /gene="groL"
                     /locus_tag="POB92_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6579922.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(2378..2924)
                     /locus_tag="POB92_002460"
                     /pseudo
     CDS             complement(2378..2924)
                     /locus_tag="POB92_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2985..3197
                     /locus_tag="POB92_002461"
     CDS             2985..3197
                     /locus_tag="POB92_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDI6579923.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            3194..3784
                     /locus_tag="POB92_002462"
     CDS             3194..3784
                     /locus_tag="POB92_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6579924.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNATVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKAREMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            4101..>4399
                     /locus_tag="POB92_002463"
     CDS             4101..>4399
                     /locus_tag="POB92_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579925.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFI"
BASE COUNT         1560 a          573 c          893 g         1373 t
ORIGIN      
        1 gtaaatgact gctttagtca gttctatgcc actatattgt acaaatggta atttttttac
       61 aatgagaagc ggtaaaaatt gagacaatat ataaataata acagttagca atgatgccca
      121 taatcttgtc ataattttcc tccaaatatt tgtttataat ttattttatc gtaaataact
      181 tgaagttaca aaacttaatt aaaaggttat gacttgaaat tttgaccaaa tttgattatt
      241 ataaatgtat gttagcactc tttaatgtta agtgctaaac tttaggtttt ttaaggagga
      301 acaatcatgc taaaaccaat tggaaatcgt gtgattattg agaaaaaaga acaagaacaa
      361 acaactaaaa gtggtattgt tttaactgat agtgctaaag aaaaatcaaa cgaaggcgtt
      421 atcgttgcag taggaactgg acgtctatta aatgatggta caagagtgac tcctgaagtg
      481 aaagaagggg accgtgtcgt gttccaacaa tatgctggta cagaagttaa acgagataat
      541 gaaacatatc tagtattaaa tgaagaagat attttagcag ttattgaata atacaaaact
      601 aaattcatag ataaattaaa aagaacgaaa atgaaatatg actaaacaaa tggaggttta
      661 tcatttatgg ttaaacaatt gaaattctct gaagatgcgc gtcaagcaat gttacgtggt
      721 gttgaccaac ttgcaaatgc agttaaagta acgattggtc ctaaaggacg taatgttgta
      781 ttagataaag agtttacagc acctttaatt acgaacgatg gtgtaacgat tgctaaagaa
      841 atcgaattag aagatccata tgaaaatatg ggagctaaac tagttcaaga agtcgcaaat
      901 aagacaaatg aaattgctgg tgacggtacg acaactgcaa cagtattagc tcaagcaatg
      961 attcaagaag gcttgaaaaa tgttacaagt ggtgcgaacc cagttggttt acgacaaggt
     1021 atcgacaaag cagttaaagt tgctgttgaa gcgttacatg aaaattctca aaaagttgaa
     1081 aataaaaatg aaattgcgca agtaggtgcg atttcagcag cagatgaaga aattggacgt
     1141 tatatttctg aagctatgga aaaagtaggt aacgatggtg tcattacaat tgaagaatca
     1201 aatggactaa acactgaact agaagtggtt gaaggtatgc aatttgatcg tggttatcaa
     1261 tcaccgtata tggttactga ttcagataaa atggttgctg aattagaacg cccatacatt
     1321 ttagtaacag ataagaaaat ctcgtctttc caagatatct tacctttatt agaacaagtg
     1381 gttcaatcta atcgtccaat cttaattgta gctgatgaag ttgaaggcga tgcattaaca
     1441 aatatcgtgc taaaccgtat gcgtggcaca tttacagctg ttgcagtaaa agcacctggt
     1501 tttggtgatc gtagaaaagc gatgcttgaa gatttagcta ttttaactgg tgcgcaagtg
     1561 attactgatg atttaggctt agatttaaaa gatgcatcaa ttgatatgtt aggtactgca
     1621 agtaaagtag aagtaactaa agataatacc actgttgttg atggtgacgg tgacgaaaac
     1681 agcattgatg cacgtgttag ccaattgaaa tctcaaattg aagaaactga atctgacttt
     1741 gatcgtgaaa aattacaaga gcgcttagct aaattagcag gcggtgttgc agttatcaag
     1801 gtaggtgcag caagtgaaac agaacttaaa gaacgtaaat tacgtattga agatgcttta
     1861 aactctacac gtgcagcagt tgaagaaggt attgttgcag gtggtggtac tgcactagta
     1921 aatgtttacc aaaaagtaag tgaaattgaa gctgaaggcg acattgaaac aggtgtgaat
     1981 attgtactta aagcattaac tgcaccagtt cgccaaattg ctgaaaatgc aggattagaa
     2041 ggctctgtca tcgttgaacg cttgaaaaat gcagaaccag gtgttggttt taacgctgct
     2101 acaaacgagt gggttaatat gttagaagca ggtatcgttg atccaactaa agtaacacgc
     2161 tcagcattac aacatgcagc aagtgttgca gcaatgttct taacgactga agcggttgta
     2221 gcatcaattc cagaaaaaaa taatgaccaa cctaacatgg gtggcatgcc aggaatgatg
     2281 taaaactgtt tatagttttg actaactaga aaattacatt atgcacgagt atgattcatt
     2341 ctaagtataa attagaatgc acgcaaatat attttaatta cgagacgtct tccatttggt
     2401 gactaaactt atgggaggcg tcttttttgt atgaatttgt gattttagca tagatattca
     2461 tagtggtatt gatatcttta tgccgtaatc gttcttgtat ttctttaatg tgtacacctg
     2521 attctatgag caatgcgcaa tgtgtgtatc tgaatgagtg cgtagagata tttttgagta
     2581 tgctggtacg tgacataatg gtttgtatcc acattgatag ttttttcatt acaatggtta
     2641 tcattatata attcgttttt ccaattattt tgaacgttta tcttataatc acgcaacacc
     2701 tttattacat ttgggtcaac ggaaatctta ccgatagaac tttcagtttt agggggaagt
     2761 atctgaaatt tcttcttgtt attatttgga ttgtaatatg ttttagtaat actaatcgtg
     2821 ttgttttcaa aatcaatatc agaccacttc aatgctagta tctcgccagc acgacagccg
     2881 gtatatgcaa gtgtgctaaa gacttcaaag ctagttaacg gggcgatgat gaaataaata
     2941 ctgatgattt ctttttaata ggtgatgaag attctgacga attgatgaca gacgataatg
     3001 tcaatgatca tattataaag aatcacatag aaatgattgt tgacagatta gcgaccgata
     3061 aagagtttta tatttttgac tcccttatac aaggacttag ttatcaagat attagtagtg
     3121 ccttagattg ttcagaacaa tctgtaatat tatggtatga aaccatatta gataaaattg
     3181 tgggggtgat aaaatgagtg agttaactgc aaagcaagca cgttttgtga atgagtatat
     3241 tagaacactg aatgtaacac aaagtgccat aaaagcagga tataacgcaa atagcgcaca
     3301 tgtgacagcg tgtaggttat tgaaaaagcc gcacatcaag caatatatac aagaacaaaa
     3361 agataagatt atagatgaga atgtattaac cgcaaaagag ttattacatg tgctaacgaa
     3421 tgcgacagtc ggtgatgaaa cagaaacgaa agaagttgta gtcaagcgtg gagaatataa
     3481 agaaaatcca caaagtggca aagtacaatt agtctataat gaacatgttg aactgataga
     3541 ggtaccaata aaacctagtg atcgtttaaa agctcgtgaa atgttgggta aatatcataa
     3601 attatttaca gataagcatg atataaacgg taatgtacct atattcatta atattggtga
     3661 atgggacgga ggtgatgagg ggttagataa ggcagtgaaa gatgtatcta acgataatcc
     3721 taatcatact gtgattgtgg atgatatacc gttagaagat tatgagtcaa ttaggttttt
     3781 ataaattggg tatatatttt atggtgttgc cagtatttat tgatagtatc ggttaagacg
     3841 atgagaagaa tgagtgagat ttaaaagagt tgtaaacgca atatcctaat gctgattttc
     3901 atattgatga tttagggagg ttttcaggag taaaagtatg atattttttt caaattacat
     3961 caacatttca gattaattat tgtaatatat aataaaatga tttaagaaaa agggagaaaa
     4021 tggataatat aaataaaaat gaaaaatttc gtgttttgtc aacaattgct attactatat
     4081 catttatttt aaattttggg ttgtttattt ctctagctat ttggtcaata aattcgatgg
     4141 gtaataaaca atgcattagt ttgaatttat tgctttcgga tgatactttc gcagtaattt
     4201 cagtaatgac aactttggga actgctattt taggaggtat agcaccttca atatatggta
     4261 tttttacatc gcctatagat aaaaaaagta gttttagggc tattagcgat tgggagcaag
     4321 aacaaagaaa taatgttaaa gatctgatga tgctattacc atttacatta cttagtattt
     4381 taattgtgat ttttatttt
//
LOCUS       DAMZLE010000172         4287 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMZLE010000172 DAMZLE010000000
VERSION     DAMZLE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.175"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(346..419)
                     /locus_tag="POB92_002464"
     tRNA            complement(346..419)
                     /locus_tag="POB92_002464"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Arg,seq:tct)
     gene            783..917
                     /locus_tag="POB92_002465"
     CDS             783..917
                     /locus_tag="POB92_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDI6579926.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            974..1108
                     /locus_tag="POB92_002466"
     CDS             974..1108
                     /locus_tag="POB92_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDI6579927.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1232..1927
                     /locus_tag="POB92_002467"
     CDS             1232..1927
                     /locus_tag="POB92_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDI6579928.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2036..2476)
                     /locus_tag="POB92_002468"
     CDS             complement(2036..2476)
                     /locus_tag="POB92_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6579929.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2671..4284
                     /gene="bshC"
                     /locus_tag="POB92_002469"
     CDS             2671..4284
                     /gene="bshC"
                     /locus_tag="POB92_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDI6579930.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
BASE COUNT         1565 a          596 c          751 g         1375 t
ORIGIN      
        1 tcaaaatcta taggaataat ataataataa aaatctctac gcatgcaaat ggtgagtgtt
       61 aaaaatcttg gtgtagtgtt ggtgtagttc aggtgcagta tagatgaaat ttgagcacaa
      121 aattacttaa ttaaaaatgt ataaacaagt gtcagacgcg tgtcaattgt gtgtcaaaaa
      181 attctataac gaataagcat gcttgtttta attactgtat tttcggggct tatgcggggg
      241 acgttttggt gacgcatcat actatattac tgaaattcaa aacaaaaaga gccccgtaat
      301 cacggaactc ttttgtttgg taatgcgtat aaaaatacct ataaacgtcc tgggagggat
      361 tcgaaccccc gaccgatggc ttagaaggcc attgctctat ccagctgagc taccaggaca
      421 cgtttaacaa cacaagaatt attatatcta aatgaactta aattagcaat accttgtaaa
      481 taaaaaatgt ttatattttt cactattata gagctattta tctaaaaagg ttcaataaga
      541 cttaaatacg aattcaggca acttaattgt gttaaataca gttttgaatg cctaactgta
      601 tttcttttct ctttaaaata cagttaagta cattataaga tgttgtgcgg ataaacaaac
      661 taattgcatc aaatttattt taaaataaca acaacaaaac gttaagcgaa taacatttcg
      721 gtgatttaaa agctacgcac gtttttgtta tcttcaaatt taaattttaa ggagtgtttt
      781 caatggaagg tttatttaac gcaattaaag ataccgtaac tgcagcaatt aataatgatg
      841 gcgcaaaatt aggcacaagc attgtgagca tcgttgaaaa tggcgtaggt ttattaggta
      901 aattattcgg attctaattt caatatgtta tgtaagtaat cagtattatt tcaaaggtga
      961 gggagagatt taaatgactg gactagcaga agcaatcgca aatactgtgc aagctgcaca
     1021 acaacatgat agtgtgaaat taggcacaag tatcgtagac atcgttgcta acggtgtggg
     1081 tttactaggt aaattatttg gattctaata taataactaa tattctttaa aataaactgg
     1141 gtgagcatac tttaatgtta tgcactcagt ttattttatt tgcagaaatt tgagcctctg
     1201 ttaagattta gatacataga caatatagga gatggggaaa ttgggatata aaaatatttt
     1261 gatagacttt gatgatacaa ttgttgattt ttatgatgca gaagaatggg cgtttcacta
     1321 tatggcgaat gtttttaatc ataaagcaac aaaggatgat tttttaacat ttaaaaaaat
     1381 caatcaccaa cattgggaag cttttcaaca aaataaatta acgaagtctg aagtattatc
     1441 agaacgattt gtgaattact tcaaacatca tcaaatggaa gttgatgggc atcgtgcaga
     1501 tgtgttattt agaaatggat tagcagaagc taaagttaaa tactttgatc aaacattaga
     1561 aacaattgtc gaattatcga aaagacatga tttatatatt gttactaatg gtgtaaccga
     1621 aacgcaaaag agaaggttaa atcagacgcc gttgcataaa tatattaaaa agatatttat
     1681 atctgaagaa acaggatatc aaaaacctaa tccggaattt tttaattatg tttttaatga
     1741 tattggtgag gatgaaagac agcactcgat tatagttgga gattctttaa catctgacat
     1801 tctaggtgga atcaatgcgg gtatagctac ttgctggttt aattttagag gatttgatca
     1861 taatccagga attatacctg attatgaaat taattcatgg aaacaactaa atgatattgt
     1921 acgttaagac aaataatttg aaaatgattt acttatatta gcttaagaaa gtaattgtaa
     1981 aaaagacact acgaaaagat gcattttcat gctcgtagtg tcgaatatta ttatcttatg
     2041 gtttccacaa taagacatca tgtccctcta tatttttagc ttccacatct gtaaatccat
     2101 gatgtaaaaa gaaatcctta gaatcatttc tgccaatggc tttaattggc atattgaaac
     2161 tctttgcaaa atcaatcaat tctgaagcgt accctctgtt ttgatatttt ggtaatactt
     2221 ctaacttcca taataatata taatcttcaa aatctgggaa gtagatttct tcgacatcac
     2281 ctttttttag taatgccatt ctagctccta attgatctcc gacaaatatg ccataaaatg
     2341 gtgaatctga acttgcatca atcatttgac cgtttaactc attgaccatg tataagtctt
     2401 tgttgccaaa cgctctaaag ttttcgaata attcgtcagt tttgtaatta atttcaagac
     2461 gtttgatttc actcatattt atctacccct tatttgttta ctactattat tatacattaa
     2521 accacttgtg tttttcattg atttgaaaat gtaaaacaat gaatattata aattttccat
     2581 gattaaattg attttcaagc cgtaaaattg tcaatcaccg gtatagactt tatactatag
     2641 tttgtaatcg cgtacttaag gagaaaaaac atggactgta aagtagttag tttaaatgaa
     2701 aaagatcagt ttataccaaa aataaagagc agtgaccctg taataacagg attatttcaa
     2761 tatgatgcag ctcaacaaac tagttttgaa aaaaggatgt ctaaagaaaa taatggaaga
     2821 gaagcggcat tagcgaatgt tattcgtgaa tatatgagtg atttaaagct ttcaagtgaa
     2881 caagaattaa acatacaaca tttagctaat ggttcaaaag ttgtgattgg tggacaacaa
     2941 gcagggcttt tcgggggacc attgtataca ttccataaaa tattttcaat cattacttta
     3001 tctaaggaat taacggatac acataagcaa caagtagtac cagttttttg gattgcagga
     3061 gaagatcatg atttcgatga agtgaatcat acatttgttt ataacgaaaa tcatgggtcg
     3121 ctgcataagg ttaaatatca tacaatggag atgccagaga cgactgtctc tagatattat
     3181 cctgataagg ctgagttgaa acaaacttta aaaacgatgt tcattcatat gaaagaaact
     3241 gttcatacac aaggtctact ggagatttgt gacagaatta ttgaccaata tgactcgtgg
     3301 actgatatgt ttaaagcact actgcatgaa acatttaaag catatggcgt tctatttata
     3361 gatgcgcagt ttgagccgtt aagaaaaatg gaagcgccta tgtttaaaaa gattttgaaa
     3421 aaacatcagt tgcttgatga tgcttttaga gcaacacaac aacgtactca aaatcaaggc
     3481 ttgaatgcga tgatacaaac agatacaaat gttcatttat tcttacatga tgaaaatatg
     3541 cgtcaattag tttcgtatga tggtaagcat tttaaattaa ataaaacaga taagacatat
     3601 ataaaggaag aaattataaa tattgcggaa aatcaacctg aattattttc taataatgta
     3661 gtgacaagac cattaatgga agaatggtta tttaacacgg tggcatttgt tggaggaccg
     3721 agtgaaatta agtactgggc tgaactaaaa gatgtatttg aactatttga tgttgaaatg
     3781 cctatcgtga tgccaaggct tagaattact tatttaaatg accgtataga aaaattactt
     3841 tcgaaataca atattccatt agaaaaagtg ttagtcgatg gtgttgaagg agaaagaagt
     3901 aagtttatta gagaacaagc atcacatcaa tttattgaaa aggtagaagg tatgattgaa
     3961 caacagcgtc gtctaaacaa agacttatta gatgaagtgg cggggaatca aaataatatt
     4021 aaccttgtga ataaaaataa tgaaattcat atacaacagt atgattattt gttaaaacgt
     4081 tatcttttaa acattgaaag agaaaacgac atcagtatga agcaatttag agaaattcaa
     4141 gaaacactcc atccaatggg aggattacaa gaaagaatat ggaatccact tcaaattttg
     4201 aatgattttg ggacagatgt gttcaagccc tccacctatc caccactttc ttacactttt
     4261 gatcatatta ttataaaacc ttaatat
//
LOCUS       DAMZLE010000173         4283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMZLE010000173 DAMZLE010000000
VERSION     DAMZLE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.176"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            27..488
                     /gene="tsaE"
                     /locus_tag="POB92_002470"
     CDS             27..488
                     /gene="tsaE"
                     /locus_tag="POB92_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI6579931.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            469..1131
                     /gene="tsaB"
                     /locus_tag="POB92_002471"
     CDS             469..1131
                     /gene="tsaB"
                     /locus_tag="POB92_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI6579932.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKILAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1104..1568
                     /gene="rimI"
                     /locus_tag="POB92_002472"
     CDS             1104..1568
                     /gene="rimI"
                     /locus_tag="POB92_002472"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6579933.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1561..2586
                     /gene="tsaD"
                     /locus_tag="POB92_002473"
     CDS             1561..2586
                     /gene="tsaD"
                     /locus_tag="POB92_002473"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI6579934.1"
                     /translation="MTKDILILAVETSCDETSVSFIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2688..>4283)
                     /locus_tag="POB92_002474"
     CDS             complement(2688..>4283)
                     /locus_tag="POB92_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDI6579935.1"
                     /translation="TFLIFVIAIIPLTSVIGIVGRYMSRQRLLKSMETLWQTISPLEA
                     FIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLYACL
                     RGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNILFLCPF
                     LPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQGYTI
                     SKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHSIQK
                     SYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELTHPLIA
                     DAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIVFTS
                     MANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASESVLS
                     FLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTRNAI
                     ELLKITSFPAKIYERAKDNVPKI"
BASE COUNT         1467 a          607 c          799 g         1410 t
ORIGIN      
        1 aaaagcataa tataggagaa tcaacattga taaagataaa taatttagat gaaatgaatc
       61 aatttgctat atttttagtt gagcaattga aaagtggtga tttgatttta cttaacggag
      121 atttaggagc aggtaaaaca acgttaacgc aatttatagg aaaagctctt ggtgtaagac
      181 gtacgattaa ttccccgaca tttaacatca ttaaatcata taggggtaaa aatttaaaat
      241 tgcatcatat ggattgttat cgcttagaag attctgatga agatttagga tttgatgaat
      301 ttttcgaaga tcaggcaatt actgttattg aatggagtca atttataaaa gatttacttc
      361 cagcgacgca tttatctatt aatatttcaa caatatctga aaatacaaga caaattgagt
      421 tgttcgcgca aggagaacat tatgaacaaa ttaaggaggc aattattcat gaattcgctg
      481 ctcattgata catcgaatca accattatca gtagcattga tgcaaaacga tgaagtttta
      541 gctgaaataa ctacggattc taaacaaaat cattcagtgc aattaatgcc tgcgattagt
      601 cagctatttg agcagagcaa aatagctaaa cagcaattag atgcgattat tgttgctgaa
      661 ggtcctggtt catatactgg cttacgtatc ggtgttactg ttgctaaaat attagcgtat
      721 gcgttagatg ttaaattata tggtgtttca tcattaaagg cattggcagc aacaatcgac
      781 catactgata aattattggt tcctgtgttt gatgcaagac gacaagcagt ttatacaggc
      841 atattccagt ggcaaaatgg tcaattagtg actattttag aagatcaata tatgtcgata
      901 gaggcattgc gagctttttt agaagattta aaccaaccat ttatttatat tgggaaagat
      961 actataaaat tacaagatga attacaaggt gaagtgattg cgcagctacc aaatgcatca
     1021 gtcatgtatc aattgataga taaaccttca gacgtacata catttactcc taaataccat
     1081 aaattagctg aggcggaacg aaattggatc aacagtcaaa agaacaatta aacattagag
     1141 agatgactaa agaagatgtg ccacaagtct ttgatattga gcgtcggagt ttcaatgaca
     1201 gttcttggac aattgacgca ttttatcatg aaatagagca aaataacttt gcgaaatatt
     1261 ttgtcttaga atttgaacaa caaattattg gttatttagg tttatggatt gttatcgatc
     1321 aagctcaaat tacaacagtt gcaatcgatg ataattatag aggctatggt ttaggtcaaa
     1381 tgcttttaaa atacggaata aactatgcaa gtcacacttg tgatgtgatg agtttagaag
     1441 taagagttaa taataaagtg gcacaacatg tatatgaaaa tttaggattt caatatggtg
     1501 gtaaacgtaa aaattattat ggtgaaggtg aggacgcaat ggttatgtgg gtgaatttaa
     1561 atgactaaag atatattaat actagctgtt gaaacaagtt gtgatgaaac aagcgttagt
     1621 tttataaaaa atggcagaga tattttatca aatacagttt taagtcagat tgaaagtcat
     1681 aaacgatttg gcggtgtcgt tcccgaagtg gcaagtagac atcacgttga aggtataaca
     1741 acaacaataa acgaggctct agtggatgcc gatgtatcaa tagaagatat tgatgccata
     1801 gcggttacag aaggccctgg actaattggt gcgttactaa taggtgttaa tgcagccaaa
     1861 gcattggcat ttgcttacga taagccactt attcctgttc atcatattgc aggacatata
     1921 tatgctaatc acatagaaga gccattaaca ttcccgctaa ttgcacttat tgtttcaggt
     1981 ggacatactg aattagttta tatgaaggat catttatcat ttgaagtcat tggtgaaaca
     2041 cgagatgacg cagtaggtga ggcttatgat aaagtggcac gaacaattgg tttaaattat
     2101 ccaggtggtc cacaagttga tcggttggct gctgaaggtg aagatactta ttcattccct
     2161 cgtgtttggt tggataaaga tagttatgat tttagtttta gtgggttgaa aagtgccgta
     2221 atcaatcaac ttcacaatca acgacaaaaa aatattccaa tcattgaagc taacgtagca
     2281 acgagctttc aaaatagtgt tgtagaggtg cttacgttta aagctattca agcttgtaaa
     2341 gaatatggtg ttcagcgatt aattgttgct ggtggcgtgg cgagtaataa aggattacgt
     2401 caatctttag cggatcaatg caaagtcaat gacattcaat taactatccc aagtcctaaa
     2461 ttatgcacag ataatgctgc aatgataggc gttgccggcc actatttgta tcagcaaggt
     2521 cgatttgctg atttagcatt aaatgggcac agcaatatag atttagaaga gtattctgca
     2581 gaataaaata aacgtcagtt attaacatga ttacgatgaa cattattgaa actgttcata
     2641 gtcttctgtc atgtgatagc tgacgttttt atatttaaag ttaaatgcta aattttcggg
     2701 acattatctt ttgctcgttc atatattttt gctggaaatg aagtgatttt taataattcg
     2761 atggcattac gtgtatttgc tttgccaggc ttaattttgt aatcaaaatg tatgttatta
     2821 ttttctatta cctcattgaa atgataattt tcataacgtt gttttaagag ttcagccaac
     2881 tcaatatcat gtgttgctgc aataactcta aagttagatt tttcatgtaa aaatgatagt
     2941 actgattctg aagcggcaat tcgttcagtt gtgttggtac ctttaaaaat ttcatctata
     3001 aagcagtata ttttttgatt atctggaatt tcaacaatac gcttaataga ttttaattca
     3061 gccataaaat aactatcgcc tgataacaca tcatcggcat ttgccattga cgtgaacaca
     3121 attccaggtt gatatacaaa tttgcttgcg gtaactgttt gtatagccga tgcaagaata
     3181 atgtttattg ctatggattt cataaatgtt gatttacccg aagcattaga cccagttaac
     3241 agtatatttt gtgatagtga gaaatcattg gctactgcat ctgctatgag tggatgtgtt
     3301 aactcagaga atactatgcc atcatttgaa tcatcaattt gtggttcagt ataacattct
     3361 agcgttctac gatacattgc taatgcataa tgattatcta attcagctat gtaatcgaaa
     3421 caatttttaa gctcattcat atgtgttgta tagctttttt gaattgaatg aaataaaaca
     3481 taatcaagca tgaaaataag tttaactagt ttaattacca tagctccgat atcttcatca
     3541 ttaacttcag ctaaaacact cgttaaatga cgtgctgttc taaattgctt aaaattgact
     3601 tcaggttgtg gagcatgttt aatttttgag atagtatagc cttgttttaa aacattggat
     3661 gcataaaaga tagattttaa gtcatcctca tatgtacgct ttaaagttgc tgataaaatg
     3721 atattaaata ggcaactcat aagaaataat aaaataccaa cttgtgaatt aataaatata
     3781 actgcaaatg agataactgg taaaaacggg catagaaaca atatattttg acgttttaca
     3841 ggtgtgattt ggtctggaaa tgttggataa acagttttac caatcaaagc taaatgatat
     3901 gttacgtttt ttctaaattc agcattatca ttaaataaac taagtaatga tttgttcgta
     3961 attgaaagca ttccacgtaa acaggcatat agtttcattt caccaatagc tgttaaatta
     4021 taattcatct tatgaaagat tgcattcata tttaagtcgg accaagtttt atcatcaact
     4081 aatgaatgtg attcaaattt ttccttgtag agcttatact catagtcgaa atgtgagttc
     4141 ggtctgataa aagcttctaa tggagaaatc gtttgccata atgtttccat agattttaat
     4201 agacgttgac gactcatgta tcgtccaaca attcctatta cagatgtaag tggaataatt
     4261 gctataacaa atattaaaaa tgt
//
LOCUS       DAMZLE010000174         4174 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMZLE010000174 DAMZLE010000000
VERSION     DAMZLE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.177"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1226
                     /locus_tag="POB92_002475"
     CDS             <1..1226
                     /locus_tag="POB92_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI6579936.1"
                     /translation="ILVIFSLAFYPAYNPKPKDLPIGILNEDKGTTIQDKNVNIGKKL
                     EDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDKDFSKDAMSKTQKVVMDSK
                     KEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTIVSEGSSLQGSQIASAVLT
                     GMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVDNEKLNKVKDHQAGGNAPF
                     LMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQMIFAVVAAFAGSFVYIYFM
                     QGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKSVPIFFILMFFSMQLVTLP
                     KQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNSTMWMFIGFMIFGAVSSLVS
                     AIVRKHSTKRTEVPS"
     gene            1433..1864
                     /locus_tag="POB92_002476"
     CDS             1433..1864
                     /locus_tag="POB92_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDI6579937.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            1969..2085
                     /locus_tag="POB92_002477"
     CDS             1969..2085
                     /locus_tag="POB92_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579938.1"
                     /translation="MVNYAIYFNNININSIMALNDNNYQFERVNERNEYRES"
     gene            2096..3130
                     /locus_tag="POB92_002478"
     CDS             2096..3130
                     /locus_tag="POB92_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6579939.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            3166..3567
                     /locus_tag="POB92_002479"
     CDS             3166..3567
                     /locus_tag="POB92_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579940.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDWLIGMGRVIGDGGTAFQIVDIAVSKSYQGQGYGSQIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKF"
     gene            complement(3843..>4174)
                     /locus_tag="POB92_002480"
     CDS             complement(3843..>4174)
                     /locus_tag="POB92_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579941.1"
                     /translation="ILFPVAFMTISITNISLASIQQKVIPEHLLARLTTIITSISAIS
                     LPMGSFIGGILIKNLGIDYTLVIGGALFIIASIIFFISTPYRSLPKIEKIEHYHIFGI
                     QKIGNNH"
BASE COUNT         1451 a          529 c          806 g         1388 t
ORIGIN      
        1 ttatcttggt aatcttttct ttagcttttt atcctgcata taatcctaaa ccaaaagatt
       61 taccaattgg tatattaaac gaggataaag gtacaacgat tcaagataaa aatgttaaca
      121 ttggtaaaaa attagaggat aaattattag atagtgattc taataaaatt aaatgggtta
      181 aggttgatag tgaaaaagac cttgaaaaag atttgaaaga tcaaaaaatc tttggagtag
      241 ctattattga taaagacttt tcaaaagatg ctatgagtaa aacacaaaaa gtagttatgg
      301 atagtaaaaa agaagaaatg caacaaaaag ttgcttcagg tgaaattccg ccacaagtgg
      361 ttcaacaaat gaaacaaaaa atggggaatc aacaagtaga ggttaagcag gctaaattta
      421 aaacgattgt aagtgaagga tcaagcttac aaggttcaca aattgcatca gctgtattaa
      481 ctggtatggg tgataatatt aatgctcaaa ttacgaagca aagtttggaa acattaacga
      541 gtcaaaatgt taaagtcaat gccgcggaca tcaatggttt gacgaatcca gtaaaagtgg
      601 ataatgaaaa acttaataaa gttaaagatc accaagcagg tggtaatgca ccattcctaa
      661 tgtttatgcc aatttggata ggttcaatcg taacgtctat cttattgttc tttgcattta
      721 gaactagtaa caatatcgtc gtgcaacatc gtatcattgc ttcaattgga cagatgatat
      781 ttgcagttgt tgcagcattt gcaggtagct ttgtttatat ttatttcatg caaggcgttc
      841 aaggatttga ttttgaccat ccaaatcgta tcgcaatttt tgtagcattt gcgattcttg
      901 gtttcgtggg ccttatttta ggtgttatgg tatggctagg tatgaagtca gttccaattt
      961 tcttcatttt aatgttcttt agtatgcaac ttgtaacgtt acctaaacaa atgttgcctg
     1021 aaagttatca aaaatatgta tatgattgga atccattcac acactatgca acaagtgtaa
     1081 gagaactatt atacttgaat catcatattg aattaaatag tacaatgtgg atgtttatag
     1141 ggtttatgat ttttggcgct gtatcaagtt tagtttcagc tattgttaga aaacatagca
     1201 caaaacgtac tgaagtgccg tcataattta ataaatagaa ataatagcta tacaagacgt
     1261 ttgtagcgag cgtcatggta tagctatttt atttgtatag cgcttgatgt aaagttattt
     1321 tagctgtcat actatgtgct aaaccaaatt cagttgtgtc atatcttcgc ttttttgtca
     1381 ctgttataat aggtataatc ggatataatg aaaaggtgag ggaggattca aaatgcgtag
     1441 gttattatat tcatttcttt tttatatgat cataggttta tttagtggct ttttctatag
     1501 agagctaaca aaagcatatg attttactgg tacaacacaa ttatcacttg tacacacaca
     1561 tacacttatt ttaggtatgt ttatgttttt aatattatta ccattggaga agttatttaa
     1621 attatcaagt tactacttat ttaactggtt cttctatgta tataacatag gtgttatcgt
     1681 tactataggt atgatggtga caaaaggatt cttccaagtc actggtaaat catactctcc
     1741 agaagcattt gcaggctttg caggtattgg gcacacaggt atgcttgcag gtttattgtt
     1801 gctatttttc cttttaagac aagcaattct taaagaacca cgtgatgagt cttttaaaaa
     1861 ataatataac tagaactaaa aaatatacac atctttcaaa ggaacattgt tgtttcttac
     1921 tttgaaaggt gtgtttttat attgtgatgg gatagttatt gataattcat ggttaactat
     1981 gcgatttatt ttaacaatat aaatattaat agtataatgg ctttaaatga taataattat
     2041 caatttgagc gcgttaatga aagaaatgaa tatagagagt cataggaggc acataatgaa
     2101 agacgttaca atcattggtg gcggtccgtc tggtttgtat gcaagctttt atgctggatt
     2161 gcgagatatg tctgtaagat tgatagatgt tcaatctgaa ttagggggta agatgagaat
     2221 ttaccctgaa aaaattattt gggatattgg cggcattgcg ccaaaacctt gtcatgaaat
     2281 tttaaaagat acaattaagc aaggattata ttttaaaccg gaagttcatt tgaatgaacg
     2341 tgtggtagat attaggaaaa aggcagaacg tcatttcgaa gttgaaacag aagcgggaga
     2401 aatatataca tcgaaagcag ttatcattgc aattggtgcg ggtattatta atccaaaaca
     2461 attagatgtt aaaggtgtag agagatatca attaactaat ttacattatg ttgtacaaag
     2521 ttacagacgt ttcaaagata aagatgtatt aatatcaggt ggaggaaata cagcattaga
     2581 ttgggcgcat gatattgcca agattgctaa aagcgtgaca gttgtttatc gaaaagaaga
     2641 tgtaagtggt catgaagcga tgaaaacgtt ggtgacagat ttaaatgtga aactatgccc
     2701 aaaaacacgt attaaatact tggtcggcaa tgatgatgaa acgcatataa gtgaagttgt
     2761 tttggaacat gttgaaagtg gcgatacgca tacagttaaa tttgacgatg tcattattag
     2821 tcatgggttt gatcgttgta atacattatt gagtgaaaca tcttcaaagc tagatatgca
     2881 tgatgattgt cgtgttaaag gatttggtaa tacgacaact agtatacctg gtatctatgc
     2941 gtgtggagat attgtttatc atgatgcaaa atcacatcta attgcaagtg catttagtga
     3001 tggtgctaat gcagcgaacc ttgctaaaac atatatccaa ccagatgcga atgcagaggg
     3061 ttatgtttca agtcatcatg aagtgtttaa agaagcgaat aagactattg taaataaaca
     3121 tttatactag gttgatatat tgtgtgatga aaagtggggt aaataatggt taaagtgaca
     3181 tatgatattc cgacttgcga ggattattgc gcattaagga ttaacgcagg tatgagtcca
     3241 aagacgcgcg aagcagctga aaaaggatta cctaatgcct tatttacagt aaccttgtat
     3301 gataaagatt ggttaattgg tatgggtaga gtaattggcg atggcggaac tgcttttcaa
     3361 attgttgata ttgctgtttc gaaaagttat caaggtcaag gttacggcag tcaaattatg
     3421 gaacatatta tgaagtatat taaaaatgta tctgtcgaaa gtgcatacgt tagtctgatc
     3481 gcagactacc cggcggataa attatataca aaatttggat ttatacctac ggaaccagat
     3541 tcaggtggaa tgtatattaa gttttaaaaa taaaaagaca tctcgcaaaa cgggatgtct
     3601 ttttgatggg aaaatatttc ccgatttaga tagatatacc aatgattaga cgaagaaaag
     3661 catttaatta actagtttta tctaataaat cattagtgat tttttgttta aaatttttgt
     3721 attcagtaaa caattcttct tgagttttga cattttgtga gtcagtttca tagatcaatt
     3781 ctaatactaa atctattttg aaaacctatt taagtatatt gaactaataa aaatgtataa
     3841 ttttaatgat tgtttccaat tttctgaatt ccgaatatat gataatgctc tattttttca
     3901 attttaggca atgatctgta aggtgtagat ataaagaaaa tgatacttgc aataataaat
     3961 aatgctccac caattacaag agtataatca ataccaagat tttttattag gattccgcca
     4021 ataaaactac ccatcggtaa tgaaattgct gaaatacttg ttatgattgt agttaatcta
     4081 gcaagcaaat gttctggaat aaccttttgt tgaatagaag ctaaggatat attggtaata
     4141 ctaattgtca taaaagctac tggaaacaaa atta
//
LOCUS       DAMZLE010000175         4151 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMZLE010000175 DAMZLE010000000
VERSION     DAMZLE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.178"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            87..353
                     /locus_tag="POB92_002481"
     CDS             87..353
                     /locus_tag="POB92_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579942.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            497..805
                     /locus_tag="POB92_002482"
     CDS             497..805
                     /locus_tag="POB92_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579943.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(913..1146)
                     /locus_tag="POB92_002483"
     CDS             complement(913..1146)
                     /locus_tag="POB92_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579944.1"
                     /translation="MLRSLCKHNRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(1127..2320)
                     /locus_tag="POB92_002484"
     CDS             complement(1127..2320)
                     /locus_tag="POB92_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6579945.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNISMIDRHSKEDFSETWEFLKKSKE
                     IPFIIIIQFACILINTGLWRVALPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLVGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISLIIPLLSLSNIFISGGAIISMLSLAGLRF
                     VKKSM"
     gene            complement(2703..>4151)
                     /locus_tag="POB92_002485"
     CDS             complement(2703..>4151)
                     /locus_tag="POB92_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HDI6579946.1"
                     /translation="HKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGP
                     KTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGE
                     GQPTEEITKQPIDKIVEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVI
                     EEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPI
                     TVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKITQGHKDIFDPNLPTDQTEKVPGK
                     PGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQE
                     GQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEK
                     PSRPTHPSGPVNPDNPGLSKDRAKPNEPVHSMDKNDKVKKSKIAKKSVANQEKKRAEL
                     PKTGAESTQKGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT         1245 a          756 c          595 g         1555 t
ORIGIN      
        1 tgttttcgcg atgaaaaagg tttagaatta caaaaataac tattttaaat ttagttacaa
       61 atatatttta gaaaggatgt gaaataatga aaggctctaa acaaatactt ttgattatgg
      121 gcattatatc tcttattgtt ttatttattt ttacactatt catcatggcg caatatgcaa
      181 aacattatga acaaaaatcc gacagttcca acgcacatac tttaaattca tcatcagcca
      241 taattgagca acatactatg tcgaatttag cgtctctaga tttatacgct cctgttcgta
      301 acattacttc tagtcgtgat attcatccca tttatttcat gacaaaagat taaaacttcc
      361 tttttcataa ccccattcat tcaactaata aattaaaaat tgataaaaac gtactgtttt
      421 ttagaaacat acatatataa gaaacgcatc cattactaaa agacagtaca ttatattcta
      481 tcgggaaggt gcaacaatga ccgtcaacat cgtgttttct atcgtgttct gtatcagtat
      541 ggttatatta ggcatttatg tcgcaataac taaagatttc acactaatct cttttataaa
      601 tcaaacgacc attgcagata aacacaagaa ccaaattgca tatatattta cgctatgtat
      661 cagtttgagt gcagtatttt tgatgtcttc aattctaagc tttgaatatg attttattgc
      721 attagcattc ttatttttaa cgatagcatt attacttatc gcattattct acgtttgctt
      781 ttataaaatt acaaaatacc cgtaaatgtt tgtgatagtg tgttggttag atttattgat
      841 gcgctgtcag taagtaattg tttgcgaaaa aaatacaacc ttgtacgaat acaaagttgc
      901 attaatatag aatcatttat gctttttaat atcacaaaat aaattgatta aactagctaa
      961 aatagtcaaa attggaatca agattacata aatatcataa cctctagata ttagtataca
     1021 taatataatt agcgacccta ttataattgc taaattataa gccaaactaa ttttatattt
     1081 catttcaatt cccaccttaa tagcattgat taaaattcta ttatgtttac atagacttct
     1141 taacaaacct taacccagct aagctaagca ttgaaataat tgcacctcct gatataaata
     1201 tattacttaa gcttagtaaa ggtataatta aacttataag tcctaaagac aatgtatccg
     1261 ctgcgtaatt cgatgtagat gagatactaa atactttccc catcaaatga tttggcgtct
     1321 ttatttgaat ggcaactgat cttgttagtc cctctataga ttgcccaagt cccactaaag
     1381 ttgcacctat atatagtatc gccacacttg gaaacacatt aattatcgat aagccaatcc
     1441 cccaaactaa aacaccaata ctaaatttaa agattaatcg tttttctgac agtaaaccca
     1501 taatcaatga cattaataaa gacgctatac ctaaacatga tgtagctagt ccatatacac
     1561 caacgccctc ttttaatata ttggaaataa acaatggtaa tgcaacacgc caaagaccag
     1621 tattaatcaa tatgcaagca aattggatga tgatgataaa tggaatttct ttagattttt
     1681 tcaagaattc ccaagtttca gaaaaatctt cttttgagtg tctatcaatc atgctgatat
     1741 ttgtatattt taaaagtgca ttaaaaataa atcctaaaaa tagcaatata ctgcaaataa
     1801 aaaagacgcc aacattacca actagtatta caatgacacc aattaaagca ggtaaaataa
     1861 tatttgagcc tctttgcaaa ctatcgatta acgcattact tgttgctaaa tgctcctcat
     1921 caataatttc aggaagaatt gccctaaacg caggatccgt atagcagtta ataatggtga
     1981 tagctgtaga tatggttaga agcgtcagat aatttaaatt tgatgttatt gcaagtaaag
     2041 gaataattat tataatcaaa cttagtataa gatcagatag ataaagtatt ttctttttat
     2101 tatgtttatc agaatatgcg ccaccgaaaa taccaaatat aataaatggt agtgtttgac
     2161 tcataaccat cattgataat tttaaagatg attggtttgt caattcaaca gtaaaccaaa
     2221 ttatttgtaa cgaaaacagc acaaagcaac tccgacgtaa gaaattacca atcaataaat
     2281 atgtaaagtt tctatttttc aaaacttcta agtacaacat atttatcacc tctcataaaa
     2341 ataattgaat gcatccacca gcttttttag accttcttct aaactctctt tatccaaagc
     2401 gcaattaatt ctaatataat ttagtcagtt aaatatcaat tatttcgaaa tttacatact
     2461 acttgaaaca ccatacataa cccccaaaat gactactcag aggttatatt ctactaatta
     2521 tgattatatt aaatatgaaa atattatcaa aaaatcaaat ttataacaaa aatacacccc
     2581 ttaaagttag gtctttcaat ccaacttttg gggtgtatat cattttcacc atattctagg
     2641 ttgtttttaa caaactaaat atagtgaatg caaatcaagt attatttaaa taatgaatta
     2701 ttttaatttt ttcttctacg tgccaataac attaatccag caattccaat tatactacta
     2761 aagatcaatc ctttttgtgt gctttctgca cctgtttttg gtaattctgc tcgttttttc
     2821 tcttgattag ctactgattt tttagcaatt ttagattttt taactttatc atttttatcc
     2881 attgaatgaa ctggttcatt tggttttgct ctgtctttcg ataatcctgg attgtcagga
     2941 tttactgggc cacttggatg agttggtctg ctcggcttct ctgggttttc aggtcctttt
     3001 ggatcttttg gtttctcgcc accaaactct acaatcttat ctactggttg ttttgtaatc
     3061 tcttctgttg gttgaccctc gccaactttt tcacccgtta atgggttcac tgtgattggt
     3121 gttgtgattg tcttacttcc tggttgtcct tcttgtttca ctcgctcttc accaggttgt
     3181 aacttcggat taaattcacg ttttgtttca aacggtatct ctactgtttt tgtttctggt
     3241 gtacccgttt ttggtccgtg tttaatcaca tcatccactg gctcttcgat cacttttcct
     3301 gtgtctggat tcttaattcc tggtttacct ggtacttttt ccgtttgatc tgttggtaag
     3361 tttggatcga agatatcttt atgaccttgc gttattttct cgccaccgaa ctctacaatc
     3421 ttatctactg gttgttttgt aatctcttct gttggttgac cctcgccaac tttttcacct
     3481 gttaatgggt tcactgtgat tggtgttgtg attgtcttac ttcccggttg tccttcttgt
     3541 ttcactcgct cttcaccagg ttgtaacttc ggattaaatt cacgttttgt ttcaaacggt
     3601 atctctactg tttttgtttc tggtgtaccc gtttttggtc cgtgtttaat cacatcatcc
     3661 actggctctt cgatcacttt tcctgtgtct ggattcttga ttcctggttt acctggtact
     3721 ttttccgttt gatctgttgg taagtttgga tcaaagatat ctttatgacc ttgcggtatt
     3781 ttctcgccac caaactctac aatcttatct attggttgtt ttgtgatctc ttctgttggt
     3841 tgaccctcac caactttttc acctgttaat gggttcactg tgattggtgt tgtgattgtc
     3901 ttacttcctg gttgtccctc ttgtttcact cgctcttcac caggttgtaa cttcggatta
     3961 aattcacgtt ttgtttcaaa cggtatctct actgtttttg tttctggtgt gcctgttttt
     4021 ggtccgtgtt taatcacatc atccactggc tcttcgatca cttttcctgt gtctggattc
     4081 ttgattcctg gtttacctgg tactttttcc gtttgatctg ttggtaagtt tggatcaaag
     4141 atatctttat g
//
LOCUS       DAMZLE010000176         4042 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMZLE010000176 DAMZLE010000000
VERSION     DAMZLE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.179"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            92..1186
                     /locus_tag="POB92_002486"
     CDS             92..1186
                     /locus_tag="POB92_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6579947.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(1276..1369)
                     /locus_tag="POB92_002487"
                     /pseudo
     CDS             complement(1276..1369)
                     /locus_tag="POB92_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1378..3114)
                     /locus_tag="POB92_002488"
     CDS             complement(1378..3114)
                     /locus_tag="POB92_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDI6579948.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(3405..3947)
                     /locus_tag="POB92_002489"
     CDS             complement(3405..3947)
                     /locus_tag="POB92_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI6579949.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWNERYKKIRQY"
BASE COUNT         1351 a          706 c          561 g         1424 t
ORIGIN      
        1 aataagcttg aattttgtaa ctagtaagtg tataacagta atgaatacga atattagata
       61 ttcaataaac ttaaaagggg cgtcattatt tttgagacta ggagctcgga tttttaaaac
      121 tggtatagcc attattttag ctatgtctat cgcttcttta ctaccggatg atgttggtct
      181 gaaagcctta gctggtgtca gtgctgttgt tgcaatgcaa cctagtattt atcgttcatt
      241 taaaacagtt tctgatcagg cattaggtaa cattataggt gccatacttt ctgttacaat
      301 ggtaacaatt tttagtgata attttatcat tatgggtgtt accgtcatag tacttattgc
      361 tattttattt aaatttaatt tagcacatgt tgctactttg gcgagtgtaa cagcattaat
      421 cattatggga caacataccg gttctttcta tattaccgcc ttttacaggt ttgtacttgt
      481 aatgattggt gtcataagtt catctctagt taactttgtg tttctaccac ctaaatttga
      541 aacaaaaatt tactataatt cattaaatat atcctcagat atttttatgt ggtttaaatt
      601 agtattaaat gatacgacgg agtttaataa tattaaacaa gatagtcata atttaaaaca
      661 acgtgtcgaa aagttagaga aaatttatga ttactatagc gaagaacgtc ctattacaaa
      721 aaaacatatt caccaacaga atagaaagaa aatacttttc agagaagtag ttcagacgac
      781 tagacaagct tatgaagtat taaacaaatt gtcgcgttat caaaacgact tatatttact
      841 taataacaat ttcctattac aaatcaaatt agatttagat tcattaactg cttttcacga
      901 gcaaatttta gctagccttt ctaaaaaagc acgttataat gtgacacatg ttgattatga
      961 gttagataat ccgcagaaaa aagatttatt gtctacattt cagcatgagt taataaatca
     1021 tccttatcaa acggaatatt cgtttgccaa tgttatgcaa attgttgcag ccatcgaaga
     1081 gtatcgacac catttagagc atttagaccg cataagaatt agctttttca catatcatag
     1141 atctgatgca gacattgaaa ttgttgaaga agactttgat ttataacatt aaaaaaattc
     1201 ctataattaa ttccacaatg agataaagta atattgaatc tcgaaattaa atttaaaaaa
     1261 gcagtaagat tattttcaat taagaaaatc tcttactgct ttttcttatg ttatgacccc
     1321 accatcctgt tacaaatttt ctcttcagga ttgtactgaa gtcaaacaac taatacttta
     1381 taagttttga atgctatata aatgctcgta agcaccttgt tttgcaatca attcacgatg
     1441 cgtacctgtt tcaacaatat gtccattttc aattacgaca attttgtcag catgtgtaat
     1501 agtggacaag cgatgcgcta cgataagtgt cgttcgatct ttactcaaca catctaatgc
     1561 ttcttgaata atggattcac tttctaaatc aagtgcactt gttgcttcat ccaagataag
     1621 aattggcgga ttatttaaaa atattctagc aatcgataat ctttgttttt gaccacctga
     1681 taatttaaca cctcgttcac ctacttcagt gtcatatccc tgtggcaagt tcataataaa
     1741 gtcatgtgca ttagccattt tcgccgcttc aactacttct tcatctgttg ctgttggacg
     1801 accaagtaaa atattttcct taactgtatc agagaataaa atattatcct gttgcaccaa
     1861 tcctatttga tttcttaaac ttcccgttaa aaaatcttta atgttgtgac catctattaa
     1921 aatttgccca gaagttacat cgtaaaatct cggtattaag ttaattaatg ttgatttacc
     1981 accaccactc atacctacga aagcaactgt ttctcctttt tcaatactca aattaatatc
     2041 ttttaaaatt ggagcttcgt tatcgttata ttgaaaacta acatgatcaa tatcaatacg
     2101 accttgttta atttcaatag gttgagcacc aacaccattt ttgatgtcat aatcttcatc
     2161 aattaattgg aatacacggt ccattgaagc aaaactttgc gttaaagttg taaatgatgc
     2221 gactaaacga cgtaaaggcc cgaacaataa ctccaagtat ccaacaaatg ctgcaagtgt
     2281 acctactgtg attgatccag aaatagcaag atatgcacca acaccgatga caattattgg
     2341 tccaatatct gtaactgtat taattgcggc aaaggaatag gcattccatc ttgtatgttt
     2401 caaagcacgt gttaagaaat tagtgttctt tttatcaaag tttttcgctt cattgtcttc
     2461 aatcgcaaaa cttttaacga ctgaaatacc ttgaacacgt tcatgcaaga atccttgaac
     2521 ctcagctaat gcttgcgatc tttcacgcgt caattttctt aatcttccaa agaaaacgta
     2581 caccgttaaa atgtaaaatg ggaagataaa cagtgctgct aaagtcaatt tcacatctaa
     2641 aaagaacatt atggatagtg caataataat tgttatacaa tctaaccaaa tattcattaa
     2701 cccggttaaa atgaaatctt ttgtttgttc aacatcatta atcactctag atattacttg
     2761 acctacttga ttattagcat aaaatctcgc acttaaagct tgtaaatggt tgtataactt
     2821 tttacgtata tcatacaata ttttattact tgtccattgc gccaaatatt gacgtataaa
     2881 ttcaattggt ggtctcacta ttacaaaaat aaataatgcg ataccaatgg caatagttaa
     2941 atgatgaact ttttcatcag tcgttagtgc gtggttatta atcacgccat ctattgcata
     3001 tttaattaaa agtggtataa gcattggtat accaaactta attatcccaa caataatcgt
     3061 tgcaaaaata cgatatttgt atggcttaac aaattgcaaa tatcgtttaa tcatacaatc
     3121 ccccctaatc tattgcccta tcctattcat aagcataaaa atgaatagag gttggataca
     3181 taatttgtag atgtaaattc ttcttacaat ttacattttt aaaattaaga tatctcaacc
     3241 tctgtcatat tcatgtgatt aaatacgttg tcttattcgt atgttatatt ctattcaatt
     3301 taatctatgg atactgtgtc cccacacgac agcaaaagtt atcatacttc tttacaacac
     3361 taagtcaata taaatgattt aatcagtatt tacactttat ttgcttaata ctgtctaatt
     3421 tttttgtaac gttcattcca aactttgata aaatctggcg cgaatgggcc cttcttctgt
     3481 tctatccatt gttgaagaat gtccacgttg cgtcttaaaa taatatcaat atcatgcgga
     3541 taattcattt gattcatatg ttgttcatat tcatcttcat ctaataaatg atactttccg
     3601 tttggatata ctttaatatc taaatcatag tctatatatt ttaatgcctc ttcatcacaa
     3661 acaaatggtg atgacaaatt gcaatagtaa taaattccat cttctctaaa catgcagata
     3721 acattaaacc aatattctga gtgaaagtaa acaattgccg gttcacgtgt tatccaagtt
     3781 cttccgtcac tttcagttac taacgtatga tcattgccac caatgacaac atgatcagta
     3841 ccctttaata ttgttgtttc agaccaaacg cgatgaatct taccatcatg tttataactc
     3901 tgaattttaa tgttttcccc ttctttaggt atggattctc tgaccatact ccacaccacc
     3961 ttctgttaat ttaaccatta taaattatag catatttcag aaatagtatt atataaatac
     4021 atatttttac gaaataagat tt
//
LOCUS       DAMZLE010000177         4017 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMZLE010000177 DAMZLE010000000
VERSION     DAMZLE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.180"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            271..752
                     /locus_tag="POB92_002490"
                     /pseudo
     CDS             271..752
                     /locus_tag="POB92_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(808..1944)
                     /locus_tag="POB92_002491"
     CDS             complement(808..1944)
                     /locus_tag="POB92_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDI6579950.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLISKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQHVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGTNKQS"
     gene            complement(1976..2605)
                     /locus_tag="POB92_002492"
     CDS             complement(1976..2605)
                     /locus_tag="POB92_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDI6579951.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(2624..2893)
                     /locus_tag="POB92_002493"
     CDS             complement(2624..2893)
                     /locus_tag="POB92_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI6579952.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            3056..3364
                     /locus_tag="POB92_002494"
     CDS             3056..3364
                     /locus_tag="POB92_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDI6579953.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            3535..3735
                     /gene="cspA"
                     /locus_tag="POB92_002495"
     CDS             3535..3735
                     /gene="cspA"
                     /locus_tag="POB92_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDI6579954.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            3932..>4017
                     /locus_tag="POB92_002496"
     CDS             3932..>4017
                     /locus_tag="POB92_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HDI6579955.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILA"
BASE COUNT         1304 a          589 c          632 g         1492 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaacgaaa aaatctatag tcaaagaaaa attgatgtag
      541 agcctgtttt tggatttatg aaggctattt tgggtttcac tcgaatgtca gttcgaggaa
      601 taaataaagt taaacgagag ctaggttttg tattaatggc acttaatata aggaaaatag
      661 cagctcaacg agctgtacat tataaaatac atatcaaaaa agctgatttc tatcaaataa
      721 ataatagaaa tcagcttttt tacattgcct aagaacttta aggaacttaa tgtcccaagc
      781 tcttttttgt tatatctaat tcgtaattta tgattgttta ttcgttcctt tgatgttcac
      841 taaatgtgac tttaaatctt gttctaattg ttgtaattct ttttcagcta attgtcgttc
      901 ttcgcgaccg tgttgttgaa taattaatgt ttcttcaatt gtctcaataa tgttacgctg
      961 tgtacgtttc aatgtatcaa ggtcaacaat gccacgctca ttttctgttg cagtttcaat
     1021 cgcattttgt ttcaacattt cagcatttgc tgttaataaa tcattagttg tatcagtgac
     1081 agctcgttgt gcagcaactg cattacgctg tctcattaat gtaagcgcaa tggccatttg
     1141 atttttccat agtggaatac ttgtcaaaat tgaactttgt atcttctcgg caagtgcttg
     1201 attaacattt tgaatcatac gaatttgtgg cgcagtttgc aaagctattt gtcttgaaag
     1261 ctgtaagtca tatatgcgtt tatctagtct atctataaat tgctgcatat ctgcaacatg
     1321 ttgaatatcc atttgattag tggattgctg cgcttgctgt tgcaattgtg gtagcttttc
     1381 attttctaat tgcaactttt tttgctgtgc agcaatgata tgcaatgata agtcatcaaa
     1441 gtattgtttg tttttatcat atagcgtatc taataattca atatctcttg ttaaatgtgt
     1501 ttgatgtttc tgcagttgaa tcgttatgcg atcgacttga gcactaactg attgcattct
     1561 tgaaaagatt tcattgatag acgactttgc tctgctaaaa attcttttta acatagatgg
     1621 tttatcagta tttaactcat ttggattaac tgactttagt tttgatatta gatctgacaa
     1681 agtatctcca ataggaccaa catctttact ttgtacttca tccaacattt gatgtgaaaa
     1741 ttgagacatt tgtttctgta aatcagaacc aaacgctaat aaaccttcat tgtctaaagg
     1801 gttaatttgt ttactgattg tgtctacctg tttttgttgt tcaattgtta acttttcttt
     1861 ttgaatagta gtattcgata attctttatc gcttataaaa tcatctagtg gatggctttc
     1921 tttgaaactt ttattttcag tcatcaatca tctctccaat agttgctatt ttacatcaat
     1981 cttgatgatg tttttgatgt aacttattta attccatttc aatatctaaa cgttcataat
     2041 catcttcgtt gagacgcttt aaatcagcga ttaatgttcg tttgacctca tccaaagtaa
     2101 ttcgtgtttg ttctaacttt tgctgttcat taattgattt tttgggcatt tttgctagac
     2161 gtgtatatgc atcaaccaaa tttaaagcat tatcaatatg agaataaaaa aagccttcaa
     2221 ctttataaaa tgatgcaggt ctctgtctaa ctgtcgtata aatagaacgt gaaatttggt
     2281 atatatcatt aatctgccta aaatctttaa ttgatcttat attgacatac gtttttaaaa
     2341 tacctctaag tttttggtgt gtatgattta actgatttcg aatatagcga tagtcttttc
     2401 tagtcaaacc aatttcgttt aaatatttgc gtgaagtgag tttttgtatc ggtaggtatg
     2461 tcattaaaaa gccaacaata ccaatagaca tatcaattaa aaaagataca tcaagtgcaa
     2521 tcatcccaaa tatgcttgtt aaaaacgcta caggaattcc cactaacacc ccaaatatat
     2581 gagaaatatt atatctcact gtcatcttcc tttatttagc attttatatt gatcgaaaat
     2641 ccgataattt ttgatttagt tctaactctt caagttgatg gcttgttaca tttgatgctg
     2701 gtgaggcacc ttcaattaca ccttgaataa atctctctat atctgcgtca tccccttgtg
     2761 catatatctc tacatagtca tctacatttt gaacagtacc gacaatgtta tagttcattg
     2821 caatgcgttg tgtaaaatat ctaaatccga cgccttgaac gcgtccgaat acttgtaaat
     2881 gtatatgtct catttttacc acctcataat gttattatac gtagttttac ttaaaaaaac
     2941 taataattac tatagttact actttgtttg tttcaagtcg tcaaacttga ttttcagagg
     3001 ataaaggtat aaaaataagt atagagtttt tgaagtatgg aaggggtctt taataatgtg
     3061 gacagttacc aaaattagag ccgattatga gggatggtgg ttattcagtg actggccaga
     3121 aaacattgtt gaaaaatatc aatatcaaga ttttgatgac atgtttaagc actatcaaca
     3181 attgattaat caatgtaaag ttcagttcga taattatgtc acaggcaaat ataatattta
     3241 tgcattttat aataattgtg acatgaacta ttgcgaagat tgtgaagagg atttacaaat
     3301 attttatagt ttcattgttt tacaaaataa tgaagtatat tataaactac caataattga
     3361 ttaaatgaat attttttatt attgtattgc aatttggtaa taactgcact ataatacaag
     3421 ttaagcagat gattattcca tattgcaaag aatatttgag tgaaatttgc atgtattttt
     3481 tgtaatatgc gaataagcat attgaatgaa ttttagtctt ggaggtttca gattatgaaa
     3541 caaggtacag ttaaatggtt taacgctgaa aaaggattcg gctttatcga agttgaagga
     3601 gaaaatgacg tattcgtaca tttttcagca attaaccaag atggttacaa atcattagaa
     3661 gaaggtcaag ctgttgagtt tgaagtagtt gaaggcgacc gcggtccaca agctgcaaac
     3721 gttgttaaac tataattctt agatttgaat cattgatttt aactaacacc ttaccaagaa
     3781 gtaaggtgtt tttatatgct tattacaaat acttaacgcg atgagtttct cagccaaatt
     3841 gagaactctt tttttatatc accaacattg tttgcaatag aaaattatat ttatataata
     3901 taattatggt gtacaacgac gaggtgattt tatgaaatat cttatcctta gtttagttgc
     3961 taatttatta gttttcggtg tattatctgc aattggatta aatattaata ttttagc
//
LOCUS       DAMZLE010000178         3986 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMZLE010000178 DAMZLE010000000
VERSION     DAMZLE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.181"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            764..1633
                     /locus_tag="POB92_002497"
     CDS             764..1633
                     /locus_tag="POB92_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI6579956.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVGGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(1704..2066)
                     /gene="mscL"
                     /locus_tag="POB92_002498"
     CDS             complement(1704..2066)
                     /gene="mscL"
                     /locus_tag="POB92_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI6579957.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            2270..3916
                     /locus_tag="POB92_002499"
     CDS             2270..3916
                     /locus_tag="POB92_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6579958.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT         1404 a          563 c          702 g         1317 t
ORIGIN      
        1 taagcaagag ttagagaatc aaattaatga agaaacatct atattgaaga atctcgaaat
       61 aaaattagat cgctacgata aaacaaaatt ggacttaaat gataaagaaa gctttataag
      121 tgaaattaaa tctgctgtaa atattggaga tcaatgtccg atatgtggta atgaaattca
      181 ggatttaggg catcatattg attttgacag tattgctaaa cgtcaaaatg aaattaaaga
      241 aattgaagca aatatccacg ctataaaatc gaatattgct gtgcataatt ctgaaattaa
      301 atttgttaat gaaaaaatat cgaatattaa tattaaaacg caaagtgatt tttcacttga
      361 agtattgaat aagcgtctgc tagaaaatga aaatgcattg aataatcaaa gagatcttaa
      421 taaatttata gaacaaatga aagaagaaaa agataatcta acgttgcaaa ttcataataa
      481 acaattgcgt ctaaataaaa atgaatctga gttgaaatta tgtcgagatc tcatcactga
      541 atttgaaaca ctctcgaaat ataataatat cactaatttt gaggtggatt ataagaagta
      601 tgttcaagat gtgaatcagc atcaagaact ctcaaaggag attgaagata agttaatgca
      661 attgtctcaa agaaagttaa ttgagcaaaa taatctaaat cattatgaaa atcaactaga
      721 aacttacaat aatgacttag aattgaatga acagtctatt gaaatggaaa tgtcgaggct
      781 gaatttaact gatgacaatg atatagatga aataatagcc tggaggggcg agcaagagga
      841 attagagcag aaaagggata cttataaaaa acgttatcat gaatttgaaa tggaaatagc
      901 taggttagaa tcattaacta aggataaaga gttattggac tctgataaat taaaagatga
      961 atatgagcta aaaaaaggaa agatgaatac actgatagat gaatactctg ctgttcatta
     1021 tcaatgtcaa aataatatta ataaaacaca atctatagtt tcgcatatta attacttaaa
     1081 tcaagaatta aaggatcaac aagaaatatt tcaattggct gaaattgtcg gtggtaagaa
     1141 taacaaaaat cttacattgg aaaactttgt cttaatttac tatttagatc aaattattgc
     1201 tcaagcaaat ctgagattag caacaatgtc agataatcga taccaactaa ttaggcgaga
     1261 agcggtttct catggtctta gtggcctaga aattgatgta tttgatttgc attcaaataa
     1321 gtctagacat attagctcgt tatcaggtgg agaaactttc caatcgtcgc ttgcattagc
     1381 tttagggtta agcgaaattg tacagcagca atcaggaggt atttcactag aatcaatatt
     1441 tattgatgaa ggattcggta cattagatca agaaacgctt gaaacagcgt tagacacttt
     1501 attaaatctt aaatcaactg gtagaatggt tgggattatt tcacatgtga gcgaattgaa
     1561 aaatagaata cctttagttt tagaagtgaa atcagatcaa tatcagagtt caacaagatt
     1621 caaaagaaat taaaacataa aaaatagaga tggtactttg gaatgtatag aacaacctta
     1681 gcccatctct ttttatttaa aaattatttt ttctcacgta ataaatctct gatttcagtt
     1741 aataacacaa cattttcttc cacaactgct tcttcttcgg cttcttcttt cttcattaat
     1801 gtatttgcaa tcttaacaaa gatgaataaa gcaaacgcga tgataataaa gtcgataaca
     1861 gattggataa ataaaccgta tttaataccc cagaatgacc attctttagc aaaatcaact
     1921 gatccgaaaa ttttaccaat taatggcata atgatatttt ctactaatga agatataatc
     1981 ttgttgaaag ctgcacccat cacaacagca attgctaaat ctaagacgtt accttttaag
     2041 gcgaactctt tgaattcttt taacattaca ctcaacctct cttttttaaa tgtaacctca
     2101 attatacaac aaataaaaaa attagaaaat agattattca agaaaatttg attatcgcaa
     2161 agacctttgt tatcctataa aggtgcgtga gttattgaat aaaatgttta gataatattt
     2221 ttattctact acataaaatt aaatatagaa ttgaagggag taattaatta tgaattcttc
     2281 ttcaccagag aatccaaatg gaaagaagta ttcaccagtc ttcatctata gtgcaattgt
     2341 tgttgctata gtcgtattac ttggtgcatt tttacctgaa caattcaact atgttaccaa
     2401 taatattaaa atgtggatta cagaaaagtt aggttggtat tatcttattc ttactacgat
     2461 tatcgtgttc ttctgtatat tccttatttt tagtcctatt ggaaaactta aactaggtaa
     2521 accaaatgac aaacctgagt ttaatacaat ttcatggttt gctatgttgt ttagtgctgg
     2581 tatggggata ggtttggtgt tttatggtgc agctgaaccg atggcgcact ttgctacgcc
     2641 acctacagca gatcccaaaa ctactgaagc ttatactgaa gctctacgtt caacattttt
     2701 ccattgggga ttccatgctt gggctgttta tggtgttgtt gcgttagcgt tggcatattc
     2761 gcaattccgt aaaggtgaac caggtttatt atctagaact ttacgtcctc ttttaggtga
     2821 taaagtagaa ggtcctattg ggatttttat tgacgtttta tctgtatttg cgacaatcgt
     2881 tggggtagcc gtttcgttag gtatgggtgc tctacaaatt aatggtggtt tacattactt
     2941 gttcaatgtt ccaaacaata cgtttgtaca agcgattatc atcattgttg ttactatctt
     3001 atttatagca agtgcatggt ctggattaag taaaggtatt caatacttaa gtaacttgaa
     3061 cattggttta ggtactattt taatggtagc tgctttaatt gttggaccaa ctgttcttat
     3121 tttaaatatg ttaactagct ctacgggtag tttactaaac acattcttgt ttaatagttt
     3181 tgatacagca gctttaaatc ctcaaaaacg tgaatggatg tcttcatgga cactttatta
     3241 ctggggttgg tggttaagtt ggagtccatt cgttggagtg tttattgcac gagtttcaaa
     3301 aggacgttca attagagagt tcatttctgg tgtcttgcta gttccagcaa ttgttagttt
     3361 tgtttggttt agtgtctttg gtgtattagg catcgagaca ggtaagaaac acaaagaaat
     3421 ttttgatatg actcctgaaa cacagctatt tggagtgttt aatcatgtgc catttggcat
     3481 tgttttatcg ttgattgcat tattattaat tgcatcattc tttattacat ctgctgactc
     3541 agcaacattt gtattaggaa tgcaaacaac atttggttca ttaaatccat ctagtatggt
     3601 aaaagttgtt tggggaattt cacaggcctt aatagcattt gtacttttat tagctggtgg
     3661 cggtaacggc gctgaagctt taaatgcgat tcaaagtgct gcaattataa gtgcattccc
     3721 attctccttt gtcgtcatac tcatgatggt aagtttctac aaggatgcga accaggaacg
     3781 taaattccta ggtttaacat tgactccgaa taaacatcgc ttacaagaat atatcaagag
     3841 tcaacaagaa gattatgaat ctgacattct tgaaaagcgt cagtcacgta gaaatataga
     3901 gaaaaaagat aactaataaa gtttagttaa gtattttaat aacaagtagt atgtcattct
     3961 agtagctaga acagatatac tacttg
//
LOCUS       DAMZLE010000179         3973 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMZLE010000179 DAMZLE010000000
VERSION     DAMZLE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.182"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            100..1101
                     /gene="argF"
                     /locus_tag="POB92_002500"
     CDS             100..1101
                     /gene="argF"
                     /locus_tag="POB92_002500"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6579959.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDNI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1124..2056
                     /gene="arcC"
                     /locus_tag="POB92_002501"
     CDS             1124..2056
                     /gene="arcC"
                     /locus_tag="POB92_002501"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6579960.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2228..3784
                     /locus_tag="POB92_002502"
     CDS             2228..3784
                     /locus_tag="POB92_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDI6579961.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     LLITQVLIYS"
BASE COUNT         1361 a          559 c          776 g         1277 t
ORIGIN      
        1 taattattga taaaatatta caaattttaa tagtaggttg tgtttatttt gtatgcgctt
       61 acaatttagg cgtaactaaa ataaaaggag ttgttattaa tgaaaaattt acgaaacaga
      121 agttttttaa ctttattaga cttttcacga caagaggtag aattcttatt aacactctcc
      181 gaagatttaa aacgtgctaa atatattggc actgaaaagc ctatgttaaa aaataaaaat
      241 attgcactgt tatttgaaaa agattctaca agaacgcgat gtgcatttga agttgcagcg
      301 catgatcaag gtgcaaatgt aacttattta ggcccaactg gatcacaaat gggtaaaaaa
      361 gaaacaacta aagatactgc acgtgtgctt ggtggaatgt atgatggcat tgaataccgt
      421 ggtttttcac aaagaacagt agaaacttta gctgagtatt caggcgtacc agtgtggaat
      481 ggtttaactg atgaagatca tcctactcaa gttcttgctg atttcttaac agcaaaagaa
      541 gtcttaaaaa aagattatgc agatattaac tttacatatg ttggagatgg tcgtaataac
      601 gttgcaaatg cattaatgca aggtgctgcc attatgggta tgaacttcca tttagtttgt
      661 ccaaaagaat taaatccaac agatgaatta ttaaatcgct gtaaaaatat tgccgctgaa
      721 aatggtggca acatattaat cacagataat attgaccaag gtgtaaaagg ttcggatgta
      781 atttacactg acgtttgggt atcaatgggt gaacctgatg aagtatggaa agaacgactt
      841 gaattattga aaccatatca agtaaataaa gaaatgatgg ataaaactgg taatccaaat
      901 gttatttttg agcattgctt accatctttc cataatgctg atacgaaaat tggtcaacaa
      961 atttttgaaa aatatggaat tcgagaaatg gaagttacag atgaagtatt cgaaagtaaa
     1021 gcttcagttg tattccaaga agctgagaac agaatgcata caatcaaagc agtgatggtt
     1081 gctacattgg gtgaatttta aatgatataa ggaagtgaat atgatggcga aaatagtagt
     1141 agcattaggt ggtaatgctt taggaaaatc acctcaagaa caactcgagc ttgttaaaaa
     1201 tactgcgaaa tcattagtag gattaataac aaaaggacat gagattgtta ttagtcatgg
     1261 taatggacca caggttggaa gcattaattt gggacttaac tatgctgcag aacataacca
     1321 aggtccggca tttccatttg ctgaatgtgg cgcaatgagt caagcttaca tcggctatca
     1381 attacaagaa agcttacaaa atgaattgca ttctattgga atggataaac aagtggtaac
     1441 actagtgaca caagttgaag ttgatgaaaa tgatccggca tttaacaatc cttcaaaacc
     1501 aattgggtta ttttacaaca aagaagaagc tgaacaaatt caaaaagaaa aaggatttat
     1561 atttgttgaa gatgctggaa gaggatatag gcgcgttgtt ccttcaccac aacccatctc
     1621 tattattgaa ttagagagta ttaaaacact tattaaaaat gatacactcg ttattgctgc
     1681 tggcggtgga ggtataccag taattagaga gcaacatgat ggttttaaag gtattgatgc
     1741 agttatagac aaagataaaa caagtgcatt gttgggtgct aatattcaat gcgatcaatt
     1801 gattatttta acagcaattg attatgtata tattaatttt aacactgaaa accaacagcc
     1861 tttgaaaaca acaaatgttg atgaattaaa acgatatata gacgaaaatc aatttgcaaa
     1921 aggaagtatg ttaccaaaaa ttgaagcagc catatcattt attgaaaaca atccaaaagg
     1981 aagcgtgctt ataacatcat taaatgaatt agatgctgcc ttagagggta aagtaggtac
     2041 tgtgattaaa aagtaattga attgaaacgc ttttcaatta ctatatgtca aatgcatgat
     2101 ttttattatt tatgtgcacc ccctgaaaat aatgcctcta ttttgatgcg gggtgcactt
     2161 tcttaattta tatttataaa atctttaagg tagaaatgct aggttaaatg tcgaaggaga
     2221 tgaaaccgtg gaaaatacaa ttaatgaaag tgaaaagaaa aaacgattta aattaaaaat
     2281 gccaggtgca tttatgattt tattcatttt aacggttgtt gcagttatag caacatgggt
     2341 tattcctgct ggtgcatatt ctaaactttc ttacgaacct tcatcccaag aactaaagat
     2401 agttaaccct cataaccaag tgaaaaaggt tccgggtacg caacaggaac tagacaaaat
     2461 gggagttaaa attaagattg aacaatttaa atcaggtgca attaataagc cggtatcaat
     2521 tccgaatact tatgaaagat taaagcaaca tccagctgga ccagaacaaa taacaagtag
     2581 catggttgaa ggtacgatag aagcggtcga tatcatggta ttcattcttg tactaggggg
     2641 acttattggc gtagttcaag ccagtggttc ttttgaatcg ggattgttag ctttaacgaa
     2701 gaaaacaaaa gggcatgaat ttatgctaat tgtgtttgta tcaatactaa tgattatcgg
     2761 cgggacgtta tgtggtattg aagaagaagc tgtagcattc tatccgattt tagtccctat
     2821 atttatagcg ttaggatacg attctatcgt ttcagttggt gccatattcc ttgccagctc
     2881 tgtcggtagt acattttcaa ctattaaccc gttctcggtt gtaattgcct ctaatgccgc
     2941 tggtacaact tttacggatg gcttgtattg gagaataggt gcttgtattg tcggtgcgat
     3001 ttttgttatt agttatttat attggtactg taaaaaaatt aaaaacgatc ctaaagcgtc
     3061 atattcttat gaagacaaag atgcttttga acagcaatgg tctgtattaa aagatgacga
     3121 tagtgcccat tttactttgc gtaagaagat aatccttaca ttatttgtac taccatttcc
     3181 aattatggta tggggagtta tgacgcaagg ttggtggttc ccagttatgg cttcagcatt
     3241 tttaatattt acaattataa taatgtttat tgctgggaca ggtaaatctg gattgggaga
     3301 aaaaggaact gtagatgcat ttgtcaatgg cgcatcaagt ttagtaggtg tatctttaat
     3361 tattggttta gctcgaggta ttaatttagt gttgaatgaa ggtatgattt cagatacaat
     3421 cttacacttt tcatcatctt tagttcaaca tatgagtgga ccattattta tcatcgtatt
     3481 actatttatt ttcttctgtt taggttttat cgtgccatca tcttctggat tagcggtatt
     3541 atcaatgcct atctttgcac cactagctga tacagtaggt ataccaagat tcgtcatcgt
     3601 tacgacatat caatttggtc aatatgcgat gttattctta gcgccgactg gacttgttat
     3661 ggccacacta caaatgttaa acatgcgata ttcacattgg ttccgatttg tatggccggt
     3721 agttgctttt gtattgattt tcggtggcgg actactaatt acgcaagtac taatttattc
     3781 ataatttgaa atgctatatt ataaaaatac taaatgggtt ttatgcatct cgtaggtttg
     3841 tagaaatact aaactaagcg aggtgcatta ttatttttga ttaagaaaat aatgacggta
     3901 atcataacac tagtaagtaa ttgatacaat actttattta ataatgatat ttttaaaatt
     3961 tgtttttaat gta
//
LOCUS       DAMZLE010000180         3885 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMZLE010000180 DAMZLE010000000
VERSION     DAMZLE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.184"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..324
                     /locus_tag="POB92_002503"
     CDS             <1..324
                     /locus_tag="POB92_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HDI6579962.1"
                     /translation="KRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKY
                     LNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEIS
                     IMLAS"
     gene            complement(990..1640)
                     /locus_tag="POB92_002504"
     CDS             complement(990..1640)
                     /locus_tag="POB92_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6579963.1"
                     /translation="MNQDIKSLVETIVPQLEYLSDKQRRVIESAIALFSEQGFDKTST
                     KEIAQRANVAEGTVFKQFKSKRMLLYAGLIPILRDHIAPVAVKQFTDELNEVTHFDAF
                     INLFVENRSKFIYDNRRILKVILNEAITNEDFQNILVNIFTHKLTSKLKDKIEWFIDN
                     GDMRNVKPEFFIRTVVAQILNLNIPIIVNNDYTKGENYQQFALFVKEGLYRMFKRE"
     gene            complement(1650..2780)
                     /locus_tag="POB92_002505"
     CDS             complement(1650..2780)
                     /locus_tag="POB92_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6579964.1"
                     /translation="MNSLHIAGRIFKQTIRDVRTLALLLIAPILLLSLLYYIFTVADN
                     TNGVTVGVHDVPDSLMTELHDKDIHVKHYKNDNDISDKIKDDKLTGFLHSDGQKVSVT
                     YANDNPTQAGELTGANQKWLMSHNMNAMKDNTNKLHQALTKIQQKMPGDGGDTPHQDM
                     AKPYKLTTHYLYGSSDSTYFDMINPILIGFFVFFFTFLISGIGLLKERTSGTLERLLA
                     SPIKRSEIIFGYVFGYGSFSVIQTIVVVLYAIYILHIDLVGSIWFVLLTAILTALVAV
                     TFGILLSTFASSEFQMIQFIPLVIVPQVLFAGIIPIESMNKGLQYFSHIMPLFYTGQT
                     MQNIMIKGYGFNDIYIYLIVLFAFFIFLLILNIIGMKRYRKV"
     gene            complement(2782..3486)
                     /locus_tag="POB92_002506"
     CDS             complement(2782..3486)
                     /locus_tag="POB92_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6579965.1"
                     /translation="MIEVKNVSKSFGKQQVVDDISISFNAGEVVGLIGPSGTGKTTLI
                     QCILGMEKIDGGQVTIQEHTMPNRKILSNIGYMAQNDALYNDLTGRENLTFFARIYMR
                     DKEDIKKRVNLCSSMVQLDNDLDKKVEMYSGGMKRRLSLAISFLQNPNILILDEPTVG
                     IDPKLRQTIWKDLTKAKAEDKCIIVTTHVLDEATRCDKLVLMNQGKILATGTPDEVKK
                     QYHTDTIEGVFLNMEG"
BASE COUNT         1297 a          654 c          555 g         1379 t
ORIGIN      
        1 aaacgtctca ttaaacaatt tggtaaacct caaaaggtaa ttacagatca ggcaccttca
       61 acgaaggtag caatggttaa agtaattaaa gcttttaaac ttaaacctga ctgccattgt
      121 acatcgaaat atctgaataa cctcattgag caagatcacc gtcatattaa agtaagaaag
      181 acaaggtatc aaagtatcaa tacagcaaag aatactttaa aaggtattga gtgtatttac
      241 gctctatata aaaagaaccg caggtctctt cagatctacg gattttcgcc atgccacgaa
      301 attagcatca tgctagcaag ttaagcgaac actgacatga taaattagtg gttagctata
      361 tttttttact ttgcaacaga accaaaaaag tagataaaat aaatgaatgt ttaatcgttt
      421 ctaatgaaag tgggagtgaa actttaaaaa tatcattgtt tagtatagtg agtatagagt
      481 agtgaaagtg aaaataattg tcactacttg tcatttatat tattgcaatc aatgttacaa
      541 tatatttgag caagttagat agatggtggc taatctcatg aaaatggggt gatgcctatg
      601 gttttggttt ctactccaag aaaggaatag catgactgat tatgaaatgc taatgattgt
      661 tcttacaatc attggtctag taataatgag caataaccaa aaaaaataac catctagcat
      721 atactttggc gagtaactag aggttatttt aatcttatta gtaaaataca agccactgtc
      781 tttttaacag gctcactagg gcgacatgta ttagcatgtt gctctttttc gtatttctaa
      841 atacgtttat tataactata tattatcacg atagtaaata atttccaaat gagtaacatt
      901 aattcacaat ttttaagagt ctgtgacata aagttcttgg ctctagaaat tatgtccctt
      961 actctcttat ttcatatttt gcatgatgtc tattctcgct taaacatcct atataagccc
     1021 tcttttacga ataacgcaaa ctgctgatag ttttcaccct tagtatagtc attattaact
     1081 attattggga tatttaaatt taaaatttgt gcgacgaccg tacgtataaa aaactcaggt
     1141 ttaacattgc gcatgtcacc attatcgata aaccattcaa ttttatcttt taatttactc
     1201 gttaatttat gggtgaaaat attaactaat atattttgaa aatcttcatt agtaatagct
     1261 tcatttaaga tgactttaag aatacgtcta ttgtcataaa taaatttaga tctattttct
     1321 acaaataaat ttataaatgc atcaaaatgg gttacttcgt ttaattcatc tgtaaattgt
     1381 ttaacagcta caggtgcgat atgatctctt aaaattggaa ttaatcctgc gtataataac
     1441 attcttttac ttttaaactg cttaaatacc gttccttctg cgacatttgc acgctgcgca
     1501 atttctttag tactcgtttt atcaaatcct tgttcactga ataatgcaat agcactttct
     1561 atgacacgtc tttgtttatc gcttaaatat tcaagttgag gcacaatggt ttcaactaat
     1621 gacttaatat cttggttcat attgacctcc taaacttttc tatatctttt catgcctata
     1681 atatttaaaa tcaataagaa aatgaaaaat gcgaataaca caattaaata aatgtaaata
     1741 tcgttgaatc cataaccctt gatcataata ttttgcatcg tttggccggt atagaataac
     1801 ggcatgatat gtgaaaagta ttgtaatcct ttattcattg attcaattgg tataatgcct
     1861 gcaaatagta cttgtggcac tatgactaat ggtataaatt gaatcatttg gaattctgag
     1921 gaagcaaagg tagataataa tataccgaat gtcacagcga caagcgctgt taatattgcc
     1981 gttaatagta cgaaccatat cgaacctact aagtctatat gcagaatata aattgcatat
     2041 aatacgacaa ctattgtttg gataacgcta aaactaccat aaccgaaaac ataaccaaaa
     2101 ataatttcac ttctttttat tggagaggca agtaaacgtt ctaatgtgcc agaagtacgc
     2161 tcttttaata agccaatgcc agaaattaaa aacgtaaaga aaaagacaaa aaatccaatt
     2221 aaaataggat ttatcatatc aaaatacgta gaatctgatg aaccatataa atagtgcgtt
     2281 gttagtttat atggtttagc catatcttga tgaggcgtgt ctcccccatc cccgggcatt
     2341 ttttgttgta ttttagttaa cgcttgatgc aatttattag tattatcttt catggcattc
     2401 atgttatgac tcattaacca tttttgattt gcacctgtta gttctcctgc ttgtgtagga
     2461 ttatcgttag cataagtcac tgatactttt tgaccatcac tgtgcaaaaa tcctgttaat
     2521 ttgtcgtctt taattttatc acttatatca ttgtcatttt tataatgttt aacgtgaata
     2581 tctttatcat gtaattcagt cattaatgaa tctggtacat cgtgaacccc aactgttacg
     2641 ccattcgtat tatcggcaac tgtaaaaatg taatatagta gcgacaatag taatataggt
     2701 gcaataagta acagtgccaa tgttcttaca tctcgaatcg tctgtttgaa aatacgtcct
     2761 gctatgtgca acgaattcat tttatccctc catattcaga aatacgcctt caatcgtatc
     2821 tgtgtgatat tgttttttca cttcatctgg tgtacccgtt gccaatatct ttccttgatt
     2881 cattaatacg agcttatcac agcgtgtagc ttcatctaat acatgcgttg tcactataat
     2941 gcatttatct tcagcctttg ctttagttaa atccttccaa atcgtctgac gcaatttagg
     3001 atcaatgcca actgtaggtt cgtctaatat aaggatatta ggattttgta aaaagctaat
     3061 agctaaagat aagcgtcgtt tcattccacc agaatacatt tcaactttct tatctaaatc
     3121 attgtctaat tgaaccatgg aactgcatag gttcacacgt tttttaatat cttctttatc
     3181 acgcatataa attcttgcga aaaacgttaa attttcacgt ccagttaaat cattatataa
     3241 agcatcattt tgagccatat aaccaatatt tgataatatc tttctattcg gcattgtatg
     3301 ttcttgaata gtgacttgcc caccatcaat tttctccatg cctaaaatgc actgtattaa
     3361 tgtcgttttt ccagtacctg aaggaccgat tagacccaca acctcaccag cattaaatga
     3421 tatagatata tcatctacca cttgttgttt accaaaggat ttacttacat tttttacctc
     3481 tatcataaat tgttcctcct cacaccgata aaatgagtga ccactcactt tatctatata
     3541 ctactttttt tatattcgtt gtcaataaaa aatgaagaga ggtaaaattt tatttttata
     3601 aaaagatttt gtagtcatgc accaacaata atgactagaa ttaaaagaat cttgatacaa
     3661 gagcatttta attcagtcat ctactgtcta tataaaaata gagcctaaga caaaattatg
     3721 ccccagactc ttaacttata tatctgtata atcaattatt ttaaattcgt ctaaatcttt
     3781 atcatatttt aaaacatatt ttgatttaat agttgtttta tctagtttct ttgaagctgt
     3841 taccgtaaat ttattatcat tttgttcaac acttaatatt tttaa
//
LOCUS       DAMZLE010000181         3870 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMZLE010000181 DAMZLE010000000
VERSION     DAMZLE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.185"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..278)
                     /locus_tag="POB92_002507"
     CDS             complement(<1..278)
                     /locus_tag="POB92_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579966.1"
                     /translation="MIKNKWKIEMWFRNFSYFIHKIDSEFILEEYLEDYLPKEYRPNF
                     ICGKAQPSETIYVSYFINQDEQTVEQHTVECDKTGFFIDEIPKHFIKN"
     gene            423..1127
                     /locus_tag="POB92_002508"
     CDS             423..1127
                     /locus_tag="POB92_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDI6579967.1"
                     /translation="MKRSVESFSELHQVLKNYRDNSLWLFRGHSNLNWSLVPKAGRAP
                     YDKYDDEETFYAWKRQAMIFLKETYSDWDMLTIAQHHGLATRLLDWSFNPLVAAFFAV
                     QTYEDCDAVIYAYLKKSDMNMKGATPFKSKWIKKVKPNGLASRVVRQSGIFTIHEPPT
                     NSIEANLSEKEELHKIIIKKHYRKKLREELSYYGINELSLFPDLDGLSSHVNWIMEIN
                     PYASNNITQELNSINE"
     gene            1151..1993
                     /locus_tag="POB92_002509"
     CDS             1151..1993
                     /locus_tag="POB92_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDI6579968.1"
                     /translation="MSVLETKLKSQMSKSAKIARNMNKLPDEIDRLRKRIERINKKRK
                     PTSSNIRDLEKSNKQLVTKQQKLADLQVEYTKIEKKINETKINLQKEQSRNQKKLSSM
                     LDKNTKGNEEIMEKLLTNSDQINEISNQIKKAVNQKEIIEYDVFLSHSSLDKEDYVSK
                     ISEKLIEKGLKVFEDVKVFEIGKSQTETMNMGILNSRFVVVFLSPNFIESGWSRYEFL
                     SFLNREINEEHVIILPIWHKVSVEDVRAYNPYLVDKYALNTSDFSIEEIVEKIYQVIV
                     NSKN"
     gene            2437..2745
                     /locus_tag="POB92_002510"
     CDS             2437..2745
                     /locus_tag="POB92_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579969.1"
                     /translation="MTEINESSLSLKTVYPVGTELSIDEYEIVKNKIMVLGKEKWTNL
                     LNEPHYYYLIEDFIETDYKKTSKGGLMGVKYFNVNEILNRDCLTTEQIAKELCNKDWE
                     "
     gene            2855..3013
                     /locus_tag="POB92_002511"
     CDS             2855..3013
                     /locus_tag="POB92_002511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579970.1"
                     /translation="MYLKGVEGDTPKYAVPILETDFSDLPSTHTTVGSLDRFRDKTMS
                     INRSGSNC"
     gene            3331..>3870
                     /locus_tag="POB92_002512"
     CDS             3331..>3870
                     /locus_tag="POB92_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FusC family fusidic acid resistance EF-G-binding
                     protein"
                     /protein_id="HDI6579971.1"
                     /translation="MNKIEVYKFVKVKQLVYQLIKLYRTNDMNSHKTQKDFLLNEIND
                     IFKEKDIDISDFITSIDDVKLTKKKAEHLLNELKVYIQDFEIPSSSQLEKIFRKVKKL
                     KRPDINLIDTKEISYLGWNDNSSNRKYIVYKNLDDKFEGIYGEISPNKVKGFCKICNQ
                     ESDTSLFLNKTKHNKSSGTY"
BASE COUNT         1543 a          449 c          655 g         1223 t
ORIGIN      
        1 ttatttttta taaaatgttt agggatttca tctataaaaa aaccagtttt gtcacattcc
       61 acagtatgtt gctccacagt ttgttcatcc tgatttataa aataacttac atatatagtt
      121 tcgctaggtt gggcttttcc acatataaaa tttggtcggt attctttagg taaataatcc
      181 tctaagtatt cttctaatat aaattctgaa tcaattttat gtataaaata actaaaattc
      241 cgaaaccaca tttctatttt ccatttattt ttaatcatag tattactcac tttcaataat
      301 taaatgtata gacataatgc tagttttatt tttaagatat ttcaataatg tttactaatt
      361 tttccctata agataagata tacaatataa aagggaacaa acgttcctat tggaggggtc
      421 ctatgaaaag gtcagtcgag tctttcagtg agttacatca agttttaaag aattatagag
      481 ataatagctt atggttattt agaggtcata gtaatttgaa ttggagttta gttccgaaag
      541 ctggtcgagc accttatgat aaatatgatg atgaagaaac attttatgct tggaaacgtc
      601 aagcaatgat atttttaaaa gagacttata gtgattggga tatgctaact atagcgcaac
      661 atcacggatt agctactaga ctattggatt ggagttttaa tcctttagtt gctgcctttt
      721 ttgcggtgca aacatatgag gattgtgatg ctgttattta tgcctattta aaaaaatcgg
      781 atatgaatat gaaaggggct acgcctttta agagtaaatg gataaaaaaa gtaaagccta
      841 acggtctagc ttcgagagtg gttagacaaa gtggaatttt tactattcat gagcctccta
      901 caaattctat agaagctaat ctttcagaga aagaagaact acacaaaatc atcataaaga
      961 aacattatcg caaaaaatta agagaagagt tatcttatta tggtatcaac gaattatcat
     1021 tatttcctga cttagatggt ttatctagcc atgttaattg gataatggag ataaatccat
     1081 acgcttcaaa taatataacg caagaattaa attcaattaa tgaataagat tataaaaagc
     1141 gaggtattat atgtcagtat tagaaactaa attaaaaagt caaatgtcaa aatctgcaaa
     1201 aatagctaga aatatgaata agttaccaga tgaaattgat agattaagaa aaaggataga
     1261 aagaataaat aaaaaaagaa aacctacttc cagtaatatt agagatttgg aaaaatctaa
     1321 taagcaatta gttacaaagc agcaaaaact tgcagattta caagttgagt atactaagat
     1381 tgagaaaaaa attaatgaaa caaaaattaa tcttcaaaaa gagcagtcta ggaatcaaaa
     1441 gaaattatcc agtatgttag ataaaaatac aaaaggaaat gaagagatta tggaaaaatt
     1501 attaactaat tcagatcaaa taaatgagat atcaaatcag ataaaaaaag cagtgaatca
     1561 aaaagagata attgaatatg atgtctttct atcccattct agtttagata aagaagatta
     1621 tgtatctaag ataagtgaaa aactaataga aaaaggacta aaagtctttg aagatgttaa
     1681 agtttttgaa atcggaaaaa gtcaaacaga aactatgaat atgggtatac taaactctcg
     1741 gtttgttgta gtatttttat cccctaattt tattgaaagt ggttggagca ggtacgaatt
     1801 tttatccttt ctaaacagag aaataaatga agagcatgtt attatattac ctatttggca
     1861 taaagtgagt gttgaagatg tacgtgcata caacccatat ttagtagata agtatgcatt
     1921 gaatacaagt gattttagta tagaagaaat agtagaaaaa atttatcaag taatcgttaa
     1981 ttctaagaat tagtaatagt attggttatc tactttaaaa gcaaagaggg aattaaatga
     2041 aaaaagcagt gtttttatta ctaaatgagt atgcagaatg gtaagcaagc tactgttcct
     2101 ctattttaaa ccaaagcagt gaatggaaaa tagaaacaac ttcaatttat gaatatttta
     2161 cctcgattgg tggatttaca attaaagtag taatagttat ccaatatttt ctagttgatt
     2221 ctcttataat atgtccaatt aagtgtagat gattcaagat gatattgagg gaaggatgag
     2281 aatgtgtctt tcaggtagct catttgatga taaccttaaa cataatattt actttcatcc
     2341 tgctgaaaaa gaggttacag tttcaagccg atagaataca ttgggttata taaagacaaa
     2401 gcaataaaag ctatcggaaa agtagacaaa gtaatagtga cggaaataaa tgagagctca
     2461 ttatccttga agacagttta tcctgtaggt actgagttat cgattgatga gtacgaaata
     2521 gttaaaaata aaataatggt gcttgggaaa gaaaagtgga ctaatctatt gaacgaaccc
     2581 cattattatt acttaattga agattttata gagacagact acaagaaaac atctaaaggt
     2641 ggattgatgg gagttaagta ttttaatgtg aatgaaattt tgaataggga ttgcttaacc
     2701 accgaacaaa tcgctaaaga attatgtaat aaagattggg aatgataaat agtgaatgaa
     2761 gctataatgc aataatgtat gtataacata atcaagtaat gaatttaatg ataagagagt
     2821 atggagtaaa gaattgaatt agtttgcatg gtctatgtat cttaaaggtg ttgaaggaga
     2881 tacaccgaag tatgcagtac caattcttga aacagacttt agtgatttac catctacaca
     2941 tacgacagtc ggatcacttg atcggtttcg agataagaca atgagcatta acagaagcgg
     3001 gagtaactgt tgaattccat ttatatccag ttagatacta gagtttgaag tgataaatct
     3061 tgattcggat gctgaaataa gtaaaagagc agttactgaa tataatataa gtataaatat
     3121 gccactagat attttaagga gtatttattc acttttataa aatcatctgc taagatagta
     3181 tggaagattt ttataggagc tgattataat gcgacatata aatatgtcat acatcatacg
     3241 ctatagtact ctaactcaaa gataagaagt acttagttat gaatagcatt catttcacaa
     3301 agtacttcaa caaaaatgga ggaatatgaa atgaataaaa tagaagtgta taagtttgtt
     3361 aaagtaaagc agttagtata tcaattgatt aagttatatc gtacaaacga tatgaattcc
     3421 cataaaacac aaaaagattt tttactaaat gaaattaatg atatctttaa agaaaaagat
     3481 attgatatct cggactttat tacatcgatt gacgatgtaa aattaactaa gaaaaaagca
     3541 gaacatcttt taaatgaatt aaaagtgtac atccaagatt ttgaaatacc ttcatcaagt
     3601 caactggaga aaatttttcg taaagtaaaa aaattaaaga gaccagatat aaatttaatt
     3661 gatacaaaag aaatttcata tttaggatgg aatgataatt cttctaaccg aaaatatatc
     3721 gtttataaaa atttagatga taaattcgaa ggtatatatg gcgaaatttc accaaataaa
     3781 gtaaaaggat tctgtaaaat ttgtaatcag gaatctgata catcactctt tctcaataaa
     3841 actaaacata ataagagtag tggaacatat
//
LOCUS       DAMZLE010000182         3860 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMZLE010000182 DAMZLE010000000
VERSION     DAMZLE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.186"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            182..1696
                     /gene="hutH"
                     /locus_tag="POB92_002513"
     CDS             182..1696
                     /gene="hutH"
                     /locus_tag="POB92_002513"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDI6579972.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIESEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    1769..1985
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2075..3361
                     /gene="serS"
                     /locus_tag="POB92_002514"
     CDS             2075..3361
                     /gene="serS"
                     /locus_tag="POB92_002514"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI6579973.1"
                     /translation="MLDIRLFRNEPVTVKSKIELRGDDSKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
BASE COUNT         1408 a          544 c          772 g         1136 t
ORIGIN      
        1 tgcaattcgt caatagataa attattaaat atgcttacaa caatcttaat atcctttaac
       61 gcactacaat agtgctctga taataggtta taaatgtacg taaaaccatt gtttcaataa
      121 aaatgaaaac gtatacttca agaaggatgg gttacttaat ataaacaagg gggtaacata
      181 tatgacttta tatttagatg gtgaaacact aacaattgag gatattaaat catttttaca
      241 acaacaatca aagattgaaa ttattgatga tgcgttagaa cgtgtcaaaa aaagtagagc
      301 ggtagttgaa cgtattattg aaaatgagga aacggtttac ggtattacta caggttttgg
      361 gttatttagt gatgtacgta tagacccgac gcaatataat gaattacaag tgaatctgat
      421 acgctcacat gcctgtggac taggtgagcc attttcaaaa gaagtagcat tagtcatgat
      481 gattttacga ttgaatacat tattaaaagg tcattcaggt gccactttag aattagtgag
      541 acaattacaa ttttttataa atgaacgtat tataccgata atcccacagc aaggctctct
      601 cggtgcatca ggagatttag cgccattatc acatttagca ttagcattaa ttggtgaagg
      661 gaaagtattg tacagagggg aagaaaagga tagtgacgat gtattaagag aattaaatag
      721 acaacctttg aaccttcagg ctaaagaagg tttagcattg attaatggta cgcaagctat
      781 gacagctcaa ggtgtcatta gttatataga atcagaagat ttaggttacc aatctgaatg
      841 gattgctgca ttaacgcatc agtctcttaa tggcattata gatgcatata gacatgatgt
      901 gcacgctgtt cgtaattttc aagaacagat taatgtggca gcgcgtatgc gtgattggtt
      961 agaaggatca acattaacga cgcgacaagc agaaatacgt gtacaagatg catatacgtt
     1021 gcgttgtata ccacaaatcc atggcgcgag ttttcaagta ttcaattatg ttaaacagca
     1081 attagaattt gaaatgaatg cggctaatga taatccactt atatttgagg aagcaaatga
     1141 aacgtttgtt atttcaggtg gtaacttcca tggacaacct attgcttttg cattagatca
     1201 tcttaaatta ggtgtaagtg aattagcaaa cgtatcggaa cgtcgtttag agcgactagt
     1261 aaatcctcaa ttaaatggtg atttaccagc atttcttagt ccagagccag gattgcaaag
     1321 tggcgcgatg attatgcaat atgctgctgc aagtctcgtt tctgaaaata aaactttagc
     1381 gcatccagcg agtgttgatt ctatcacttc atctgcgaac caagaagatc acgtatctat
     1441 gggaactaca gctgctagac atggttatca aattattgaa aatgcaagac gtgtgctggc
     1501 aatcgaatgt gttattgcat tacaagcagc agagttaaaa ggtgttgaag gtttatcacc
     1561 aaaaacacgt cgtaaatatg aggagtttcg aagtatcgtg ccatccatta cacatgaccg
     1621 tcaatttcat aaagatattg aagcggttgc acagtattta aagcaatcaa tttatcaaac
     1681 gactgcatgt cactaaatcg acatgagcga tgtttgaaat cgttacttga aaaagcaaat
     1741 atagtttgct atattaaaaa taacttaata agacattgtt cgtaggacaa gtaatatata
     1801 gtgttcgata tcagagagct tgtggttagt gtgaacaaga atcaacatat atatgaatct
     1861 acctacttaa tttaaaagaa caatcggtga taaccgttat tttagtgaag tgcaatttag
     1921 gtttagtgta tctttataac ttaaattgtt aaatagggtg gcaacgcgta gaccacgtcc
     1981 cttgtaggga tgtggtcttt ttttattttc taaaaaataa agacgtacgt cattccataa
     2041 taaatgataa ttatttggga aaggatgaag gttaatgtta gacattagat tattcagaaa
     2101 tgagcctgtc acagttaaga gcaaaattga attacgtgga gatgattcaa aagttgtaga
     2161 tgaaatttta gaattggatg agcaacgacg taaattaatt agtgcaacag aagaaatgaa
     2221 agcacgtcgt aataaagtaa gcgaagaaat cgcattaaaa aaacgtaata aagaaaatgc
     2281 tgatgatgtg attgctgaaa tgcgcacatt aggtgacgat attaaagaaa aagatagtca
     2341 attaaatgaa attgataata aaatgacagg tatcctttgt cgtattccaa atttaataag
     2401 tgatgatgta cctcaaggtg aatctgatga agataacgtt gaagttaaaa agtggggtac
     2461 accacgtgag ttttcatttg aacccaaagc acattgggat attgtagaag aattgaaaat
     2521 ggctgatttt gatcgtgcag caaaagtttc aggtgcgcgt tttgtatatt taacaaatga
     2581 aggtgcgcaa ttagagcgtg ctttaatgaa ctatatgatt acaaaacata caacacaaca
     2641 tggttataca gaaatgatgg taccacagct tgtgaacgca gatacaatgt atggtacagg
     2701 tcaattacct aaatttgaag aagatttatt taaagtagaa aaagaaggat tatatacaat
     2761 tccaactgct gaagtaccat taacgaattt ctaccgtaat gaaattattc aaccaggtgt
     2821 acttcctgaa aaattcactg gtcaatctgc atgtttccgt agtgaagcag gatcagcagg
     2881 tagagataca agaggattaa ttcgtttaca tcaattcgat aaagtggaaa tggtacgttt
     2941 tgaacaacct gaagattcat ggaatgcttt agaagaaatg acaacaaacg cagaagcaat
     3001 tctagaagag ttaggtttac catatcgtcg tgttatttta tgtacaggtg atattggatt
     3061 tagtgcaagc aaaacatatg atttagaagt ttggttacca agctacaatg attataaaga
     3121 aattagttca tgctcaaact gtacggattt ccaagcgcgt cgtgctaaca tccgcttcaa
     3181 gcgtgacaaa gcagctaaac cagaattagc acatacatta aatggtagtg gtttagcagt
     3241 tggacgtaca tttgctgcta ttgttgaaaa ttaccaaaat gaagatggaa cagtaacaat
     3301 tccagaagca ttagtaccat ttatgggtgg taaaacacaa atttcaaaac cagttaaata
     3361 aaggctttag ctacaagctt taaaaagtat atatctacgt atacttaaag caagggcaag
     3421 atactttaaa taatatttta aaaagtggtg acgaagctgt cgccactttt tttgtgctgt
     3481 aaaagtatag tagtgagcca atgcacataa caacaataaa ttaagtttgt tgtttaatgg
     3541 ggtgaacgca tttcattata gcaacaatac gggataatta tgatgaacta aaacaatcta
     3601 aaacgtaaca attttgagca tcactattat aggaaattaa gcgataaaat actgatttcg
     3661 ttgatgtagt atgagtaata tcgatggagt agggtagggg gaggggatga tgattataag
     3721 ggagtggtac atgaatcaat atcccagact catcattaga tataaaaatt tataaaatta
     3781 gtatttaaaa acaactacaa tccatagaaa atatggaggt agtcttaaat aaaaaattga
     3841 aaattctaaa aaaataaaaa
//
LOCUS       DAMZLE010000183         3844 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMZLE010000183 DAMZLE010000000
VERSION     DAMZLE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.187"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(149..1438)
                     /locus_tag="POB92_002515"
     CDS             complement(149..1438)
                     /locus_tag="POB92_002515"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6579974.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK"
     gene            1519..1974
                     /gene="bcp"
                     /locus_tag="POB92_002516"
     CDS             1519..1974
                     /gene="bcp"
                     /locus_tag="POB92_002516"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDI6579975.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TIEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            1980..2930
                     /locus_tag="POB92_002517"
     CDS             1980..2930
                     /locus_tag="POB92_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6579976.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNDLIENEVDAKKGY"
     gene            3027..3473
                     /gene="perR"
                     /locus_tag="POB92_002518"
     CDS             3027..3473
                     /gene="perR"
                     /locus_tag="POB92_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDI6579977.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT         1415 a          561 c          636 g         1232 t
ORIGIN      
        1 tcagagatta aaagagtata aatttgtaaa ataaaaactc acatagtgac atatcaagtt
       61 aaacgttaat agttaacgat ataaaatgaa tctactatgt gagcatttgt tttattttaa
      121 ttcaattaaa aatatacttc cttaaaagtt atttcatttg actaaaagca tagtctgcag
      181 cttttaaagt ttgttgaata tcttcttctg tatgttcagt tgttaagaac caagcttcaa
      241 acttagaagg tgctaaattg ataccttgat ttaacattaa tttgaaaaat ttaccgaacg
      301 cttcgccgtc agaatgttca acttgatcat aatgtgtgac tttttcatct gtaaagtaca
      361 atgttaaaga tccataaata cgattaattg tagctgtgat attatgtttt tcgattaatt
      421 taagtaaacc ttcttctagt tgttggccta agctgtctaa tttttcataa acaccgtctt
      481 gttctagtac ttcgagtaat gcaatacctg ctttcataga taacgggtta ccagccattg
      541 taccagcttg atatgcaggt cctagaggtg ctacttgttc cataatatct tgacgtccac
      601 catagcctcc aattggtaaa ccaccgccaa caattttacc aaatgcagtt aaatcaggga
      661 taacacctaa taaatcttga gcggcaccgt aatggaaacg gaatgcagta attacttcat
      721 cataaatcac tagggtccca ttgttatgtg aaatttcatt aacctcttct aaaaatccag
      781 gttgaggcat taccattcca aagttaccaa caattggttc tactaatact gcggcaattt
      841 catcacccca aaattcaatt gcttctttat aggcgttaat atcattgaaa ggtacagtaa
      901 tgacttcacg tgcgacgctt tctggaacac cagctgagtc tggagaaccg agctgagatg
      961 ggccgctacc tgctgcaacc aatactaaat cagaatggcc atgataagat ccagcaaatt
     1021 ttataatttt atttctttta gtatatgcac gtgcaacacg aattgttgtc atgactgctt
     1081 ctgttccaga atttacaaag cgaattttct caagagatgg aattgcatca cgtaattttt
     1141 tgctgaattc aatttctaat tcagtcggtg taccaaataa aacaccttta gcagcttgtt
     1201 cttgaattgc tttagtaata tgaggatgtg catgccccgt aataattgga ccgtatgctt
     1261 gaaggtaatc aataaattta ttgccatcga catcatataa atatgcaccg tgtccttctt
     1321 tcataacaac aggtgcaccg cctcctacag ctttataaga acgagaaggg gaattgacac
     1381 cgcctagaat atattcgttt gaaagttgtt gtaaacgttc actttcacta aaattcattt
     1441 atatcaacct cttttaattt aatattttca tctaatatcg tatcataaaa ttattataat
     1501 gaagaaaaag gtgattatat gttgcaaaaa ggagaacaat ttccaatatt taaattagaa
     1561 aatcaagacg gaactgtcat tacaaatgat acattaaaag gtaaaaaggc gattatatat
     1621 ttttatccta gagataatac acctacttgt accatagaag cttgtgactt tagagacaat
     1681 ttagaaatgt tcaatgattt agatgttgca gtatatggta taagcggtga ttcaaagaaa
     1741 aaacaccaaa attttattga gaaacacgga ttgaatttcg atttattagt agatgaagat
     1801 tttaaattag ctaaagaaac tggcgtatat cagttaaaaa aatcatttgg caaagaaagt
     1861 atgggcattg taagaacgac ttttataata gatgaacaag gtaaagtatt agatgttatc
     1921 gagaaggtta aggttaaaac acaaatagaa gaacttaaaa acattttggg gtgacatata
     1981 tgaaagttgt tgggttgaat cgtatgcgtg aagttgaaac tgaattacaa caacgctttt
     2041 cagatttaga ttttaaattt tataaaaaag catcagaaat acctgagagc gacttggctg
     2101 atttagatat attagttggt tatgatggcg gtatcaatga ggcattttta caacgttgcc
     2161 cgaatttaaa atggattgca tggtttgcaa cgggtgtaaa tacattgccg ttagattata
     2221 ttgcagatca cggcatactt ttaactaatg gaaaaggtgt tcaagctaaa caattatctg
     2281 aatacatttt agctttcatt ttagatgatt ataaaaagat gaaactatca tatgataacc
     2341 aacgacaaca tatatatgat tcgaaaataa ctggtaaacg cctatcagga caaacagttt
     2401 tatttttagg tacaggtgca attgctacta gaactgcgaa gttagcaaag gcttttaata
     2461 tgaatttaat tggtctgagc aagtcaggtc agaacaaaga tgagtttgac gagatatata
     2521 ctatcgaatc acttgaaagc acattaccaa atgctgacat tattataaat gctttaccag
     2581 aaacgcaaga aacgattcat ttactaaaga aaaaacattt tgaattaatg aaagatgaag
     2641 cactttttat aaatatagga cgaggtagca tagttaaaga agcgctctta atagaagtat
     2701 taaaaagtaa agttattcga catgcatatt tagatgtgtt tgaaaatgaa cctttgaaac
     2761 ctaatcatga attatatgaa ttggataatg taactataac agcgcatata actggtaatg
     2821 attatgaggc aaagtatgac ttattagata tttttaaaaa caatctagtt aattttctca
     2881 ataagaatga tctaattgag aatgaagttg atgctaaaaa aggctattaa atgaaatcat
     2941 catgtaaata ttgacacgcg cgcaatacta cagttatatt tatagtaagt aataataatt
     3001 attatataag aaagatggtg atatagatga gtgttgaaat agaatcaatt gaacatgaac
     3061 tagaagaatc aattgcatca ttgcgacaag caggcgtaag aattacacct caaagacaag
     3121 caatattacg ttatttaatt tcttcacata ctcatccaac agctgatgaa atttatcaag
     3181 cactttcacc tgattttcca aatataagtg ttgcgacaat atataataac ttaagagtgt
     3241 ttaaagatat tggaattgta aaagaattaa catatggaga ctcatcaagt cgattcgact
     3301 ttaatacaca taatcattat catattatat gtgaacaatg tggtaagatt gttgattttc
     3361 aatatccaca gttaaatgaa attgaaagat tagctcagca tatgactgac tttgacgtaa
     3421 cacatcatcg aatggaaatt tatggagttt gtaaagaatg ccaagataaa taatttaact
     3481 ttggtagtat gacaaattaa aaaggcgtta gcttacttca tataagtaag cgtaatattt
     3541 aagaagttaa acgacatgaa agttgtttaa cttctttttt atgcttattc tgaaaaatat
     3601 ttaaatagaa gaaaagaaca ttctaactac ggaagtggta ttaactaata actagtatcc
     3661 tttataaata aacataaaaa tattcaaacg aaggtctact aaataactat ccataaagtg
     3721 agtataagtt tgtttgtaaa actgaagtaa aaagcgtaag gaattacact ttaaatacat
     3781 atagtactta cgaatagaaa ataatccctt caacagtaat ttttaaacaa aaataagtgt
     3841 tgtt
//
LOCUS       DAMZLE010000184         3803 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMZLE010000184 DAMZLE010000000
VERSION     DAMZLE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.188"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..424
                     /locus_tag="POB92_002519"
     CDS             <1..424
                     /locus_tag="POB92_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6579978.1"
                     /translation="NKKTEKYILSKGDLAMVLNDKTKDGKIIGRSIFIDKDNQYIYNQ
                     RTERLIPFAENDNKFLWFLMNTDLIRNKIKGMMQGATQVYINYSSIKLISIQLPLLEE
                     QQKIRGFLEVLSGITTKQLHKIDQLKERKKAFLQKMFI"
     gene            830..1522
                     /locus_tag="POB92_002520"
     CDS             830..1522
                     /locus_tag="POB92_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HDI6579979.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKAIEQSRLSVTSKDTQ
                     ELKKYYSGTGYNFQNVSGYREGNKMNIIDGPQLNVVTLLGTDKERFKDDEDYEGLDVF
                     VVREGSGKHADNISIGGITKTNKNQYKDPVQNVNLLTSKSNGQNTASVTSEYYSINKE
                     EISLKELDFKLRKQLIDKHDLYKTEPKDSKIKVSMKNGGYYTFELNKKLQPHRMGDTI
                     DSRNIKKIEVNL"
     gene            1544..3046
                     /locus_tag="POB92_002521"
     CDS             1544..3046
                     /locus_tag="POB92_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDI6579980.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGEAEASEGSQTVKNPKV
                     NATEEIKVGSQPVQNNQEVSSEQTKKNFVNLDPIKPGAQKVTGTTLPSHIILMNIDGK
                     SADSVDGGNSDLVFADENGRFEYPLHNRKIVHNQEIEVSSSSPDLGDDEEDEEVEEDS
                     TEKAGTEEENTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHT
                     SVKGKVALSINNKFINFEERAKDGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDN
                     LELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDV
                     KEDLHVDEIYGSLYHTEQGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQ
                     VDENGKFSFDAEKAGFRLQNGETLNFAVVKPITGELLHQGFVSKYIDVYESPEEKKER
                     EFEEKLENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERKAPKLEK"
     gene            complement(3153..3461)
                     /gene="spn"
                     /locus_tag="POB92_002522"
     CDS             complement(3153..3461)
                     /gene="spn"
                     /locus_tag="POB92_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDI6579981.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYSNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         1536 a          458 c          659 g         1150 t
ORIGIN      
        1 aaataaaaaa actgaaaaat atattttatc aaaaggcgac ttagcaatgg tattaaatga
       61 taaaacaaaa gatgggaaaa ttataggtag aagtatattt atagataaag ataatcaata
      121 tatttataat caaagaactg aaagattaat accatttgct gaaaatgata ataaattttt
      181 atggttctta atgaatacag atttaattag aaataaaata aaaggtatga tgcaaggagc
      241 aacccaagtt tatataaatt attcatctat taaattgata tctatacaat tgccacttct
      301 tgaagaacaa cagaaaataa gagggtttct agaagtttta tctggaataa ctactaaaca
      361 attgcacaag atagaccaat taaaagagag gaaaaaggcg tttttacaga aaatgtttat
      421 ttgatttgtc gctgcaatat agtttttatt atttgtttat ttcagatgtt tcaccatcat
      481 attgcgtaac ttttacaaat aagaaataaa gttcaatgaa atcaaaaacg aacattaaat
      541 ttaggcactg tgatagcaca gtgtcttttt tgtgtcgaaa ttgtgtacag aataagtagt
      601 taaataaaga ttaagttgag ataaagtgtt attcgtaaat aaaagagagt agatcgatag
      661 gaattgaatg atattagtta actatttatt aaattactta ataatgatta atttttagtt
      721 aaagtaagtt taatgtgaag cacgaccatt gctcattata atgaatgagg attgttcgta
      781 ttgcgtaata gaataaatca aatagactaa aaattgggag catagaatta tgaaattaaa
      841 aaatattgct aaagcaagtt tagcactagg gattttaaca acagggatga ttacaactac
      901 tgctcagcca gtaaaagcaa ttgagcaaag cagattatca gttacttcaa aagatacaca
      961 agaattaaaa aaatactaca gtggaacagg atataatttt caaaatgtga gtggttatag
     1021 agaaggtaat aaaatgaaca ttattgatgg accacaactt aatgtagtta ctttacttgg
     1081 cacagacaaa gaaaggttta aggacgatga agattatgaa ggacttgatg tatttgttgt
     1141 aagagaaggg tcaggtaaac acgcagataa tatatcaatt ggtggaatta caaaaacaaa
     1201 taagaatcaa tataaagacc ctgtacaaaa cgttaattta ttgacttcta agagtaacgg
     1261 tcaaaatact gcttctgtga cttcagaata ctatagcatc aataaagaag aaatttcatt
     1321 aaaagaactt gatttcaaac taagaaagca attaattgat aaacatgatc tttataagac
     1381 agagcctaaa gacagcaaaa ttaaagtttc tatgaaaaat ggcggctact atacgtttga
     1441 attaaataaa aaattacagc ctcatcgcat gggtgatacg attgatagta gaaatataaa
     1501 gaaaattgaa gtgaatttat aataatattc gagggagtat atcatgagag aaaattttaa
     1561 attacgtaaa atgaaagtcg gtttagtatc agttgcaatt actatgttat atatcatgac
     1621 aaatggcgaa gcagaagcat ctgaaggtag ccaaactgtt aagaacccaa aggtgaatgc
     1681 aactgaagaa atcaaagttg gatcacaacc agttcaaaat aatcaagaag taagttcgga
     1741 acaaacaaaa aagaattttg ttaatttaga ccccattaaa cctggtgctc aaaaggtaac
     1801 agggactact ttacccagcc atattattct aatgaatata gatggtaaaa gtgctgattc
     1861 agtagatgga ggaaatagtg atttagtatt tgctgatgaa aacggaagat tcgagtatcc
     1921 actacacaat agaaaaattg ttcataatca agaaattgag gtttcgtcat ccagtcctga
     1981 tttaggtgat gatgaagaag atgaagaagt agaagaagat tcaactgaaa aagctggtac
     2041 tgaggaagaa aacacagatg ctaaagctac atacacaaca ccacgatatg aaaaagcgta
     2101 tgaaataccg aaagaacaac taaaagaaaa agatggacat caccaagttt tcatcgaacc
     2161 tattactgaa ggttcaggta ttattaaagg tcatacgtct gtaaaaggta aagttgctct
     2221 atctattaat aataaattta ttaattttga agaaagagct aaagatggaa ttagtaaaga
     2281 agatactaaa gctagttccg atggtgtttg gatgcctatt aatgaaaaag gatattttga
     2341 ttttgatttc aaaaaaaatc cttttgataa cttagagtta aagaaaaatg atgaaatctc
     2401 attaacattt gcacctgatg atgaagatga agcattgaag tcattaattt tcaaaactaa
     2461 agtaacgagt ttagaagata ttgataaagc agaaactaaa tatgaccata ctaaagtgga
     2521 aaaagtaaaa gtattgaaag atgttaaaga agatttacat gtagatgaaa tttatggaag
     2581 tttgtaccat acagaacaag gtaaaggtat tctcgataaa cagggaacta aagaaattac
     2641 aggtaagact aaattcgcga atgcagtagt gaaagtatat tctgacttag gtgatgcgca
     2701 actgtttcct gatattcaag tagatgaaaa tggtaaattt agctttgatg ctgaaaaagc
     2761 tggtttcaga ttacaaaatg gagaaacatt gaattttgca gtagttaaac ctattactgg
     2821 tgagctatta catcaaggat tcgtttctaa gtatatcgat gtttatgaat ctccggaaga
     2881 aaagaaagaa cgtgaatttg aagagaaact tgaaaacacg cctgcatatc ataaattaca
     2941 tggtgataaa attgtcggct atgatgttca aggtaatcca tcaacttggt tctatccatt
     3001 aggtgaaaag aaagttgaac gtaaggcacc aaaattagaa aaataattaa taaaataaaa
     3061 cagcttaatg atgtaatgaa actagtgagt taatcactga cttcaacgtc attgagctgt
     3121 tttttgtgct ttgttacaaa gcattattga gtttatttta cgtgttcata ttttgaaaca
     3181 tcaaagccgt cttgtttagc tttgttgata atgtctttga ttgaatgtag tcctttatcg
     3241 gcgaagtatg atcttaagtt gtcttttgta gcttggtcag cattcttatc taataacaca
     3301 tcgatatagc ttaattcatg ttctaagaag tttgcatcat catgtagtac aagtccattt
     3361 tgagaataaa ctttcgcatc tgcttgatta ctatatccaa caacgccagt tgctaaaaca
     3421 cctaccattg ccgtagctac taaaaccttt ttaaatttca tatctatcac tcctctaaaa
     3481 aatgtaactc tatcataaca ctgaatattg agaaaattac gtttattaag ccgatttaat
     3541 aattttaata aatagttaaa gtgacaaata ttgtttaaat gcaattaatc tttaatgcga
     3601 tggttatgga ttttcctact aaaaccttga tttcaaagag ggtttaaatc aaatgaaaca
     3661 ataataaaaa aatgacgcaa cataataata agtacaaatt taattaagaa attaaattga
     3721 ttgtatatgt atattttggt aacgtaaaag agaaatatac aaaataatta attatttata
     3781 tgaaaagaga atataaatga agt
//
LOCUS       DAMZLE010000185         3739 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMZLE010000185 DAMZLE010000000
VERSION     DAMZLE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.189"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            89..1558
                     /locus_tag="POB92_002523"
     CDS             89..1558
                     /locus_tag="POB92_002523"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI6579982.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            1551..2372
                     /gene="nadE"
                     /locus_tag="POB92_002524"
     CDS             1551..2372
                     /gene="nadE"
                     /locus_tag="POB92_002524"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI6579983.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            2643..3245
                     /locus_tag="POB92_002525"
     CDS             2643..3245
                     /locus_tag="POB92_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDI6579984.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            3226..3399
                     /locus_tag="POB92_002526"
     CDS             3226..3399
                     /locus_tag="POB92_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDI6579985.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
BASE COUNT         1352 a          491 c          724 g         1172 t
ORIGIN      
        1 gtatggaaat taaggagctc ttatgtatat aaaaaaatga attatgataa aatgtaagaa
       61 aatatttagg tcgattggag agatacaagt gtaccaatta gaagacgaca gtttaatgtt
      121 acataatgac ttatatcaaa taaatatggc tgaaagttat tggaatgata atattcatga
      181 aaaaatggct gtatttgatt tgtattttag aaaaatgcca tttaatagtg gctatgctgt
      241 ttttaatggt ttaaaacgcg tcattgattt tatagaacat tttggatttt cagaatctga
      301 tttggaatat ttaaagtcta ttggctacaa ggatgatttc ttatcatatt taaaagattt
      361 aaaattcaca ggaagcattc gttcgatgca agaaggcgag ttatgctttg gtaacgaacc
      421 attgttacgc gtagaagcac cattgattca agcgcaatta atagaaacaa ttttattaaa
      481 cattgtaaat ttccatacac taattacaac aaaggctagc agaattcgtc aaattgcatc
      541 aaatgataaa ttaatggagt ttggtacacg tcgtgcgcaa gaaattgatg cagcattgtg
      601 gggcgctaga gctgcttaca tcgggggctt tgattctaca agtaatgtta gggcggggaa
      661 attatttggt atacctgtgt ctggtacaca tgcacatgca tttgtccaaa cttatggaga
      721 cgaatatgtt gccttcaaaa aatatgctga aagacataaa aattgtgtgt tcctagtaga
      781 tacattccat actttaaaat ctggcgtgcc aaacgcaata aaagttgcaa aagaattagg
      841 tgacaaaatt aactttgtag gtattcgatt agattctgga gatatcgctt atttatctaa
      901 agaggcaaga cgtatgcttg atgaagcagg atttactgaa actaaaatta tcgcgtctaa
      961 tgatttggat gaagaaacga ttacgagttt gaaagcacaa ggtgcaaaag tagattcttg
     1021 gggcgttggt acaaagctga ttacaggata cgatcaacca gcattaggtg cagtatataa
     1081 acttgtagct attgaaaatg aagatggttc atatagtgat cgtattaaat tatcaaataa
     1141 cgctgaaaag gttacgacgc caggtaagaa aaatgtatat cgcattataa acaagaaaac
     1201 aggtaaggca gaaggcgatt atattacttt ggaaaatgaa aatccatacg atgaacaacc
     1261 tttaaaatta ttccatccag tgcatactta taaaatgaaa tttataaaat ctttcgaagc
     1321 cattgatttg catcataata tttatgaaaa tggtaaatta gtatatcaaa tgccaacaga
     1381 agatgaatca cgtgaatatt tagcactagg attacaatct atttgggatg aaaataagcg
     1441 tttcctgaat ccacaagaat atccagtcga tttaagcaag gcatgttggg ataataaaca
     1501 taaacgtatt tttgaagttg cggaacacgt taaggagatg gaagaagata atgagtaaat
     1561 tacaagacgt tattgtacaa gaaatgaaag tgaaaaagcg tatcgatagt gctgaagaaa
     1621 ttatggaatt aaagcaattt ataaaaaatt atgtacaatc acattcattt ataaaatctt
     1681 tagtgttagg tatttcagga ggacaggatt ctacattagt tggaaaacta gtacaaatgt
     1741 ctgttaacga attacgtgaa gaaggcattg attgtacgtt tattgcagtt aaattacctt
     1801 atggagttca aaaagatgct gatgaagttg accaagcttt gcgattcatt gaaccagatg
     1861 aaatagtaac agtcaatatt aagcctgcag ttgatcaaag tgtgcaatca ttaaaagaag
     1921 ccggtattgt tcttacagat ttccaaaaag gaaatgaaaa agcgcgtgaa cgtatgaaag
     1981 tacaattttc aattgcttca aaccgacaag gtatcgtagt tggcactgat cattctgcag
     2041 aaaatataac tggtttttat acgaagtacg gtgatggtgc tgcagatatc gcacctatat
     2101 ttggtttgaa taaacgacaa ggtcgtcaat tattagcgta tcttggtgcg ccaaaggaat
     2161 tatatgaaaa aacgccaact gctgatttag aagatgataa accacagctt ccagatgaag
     2221 atgcattagg tgtaacttat gaggcgattg ataattattt agaaggtaag ccagttacgc
     2281 cagaagaaca aaaagtaatt gaaaatcatt atatacgaaa tgcacacaaa cgtgaacttg
     2341 catatacaag atacacgtgg ccaaaatcct aatttaattt tttcttctaa cgtgtgactt
     2401 aaattaaata tgagttagaa ttaataacat taaaccacat tcagctagac tacttcagtg
     2461 tataaattga aagtgtatga actaaagtaa gtatgttcat ttgagaataa atttttattt
     2521 atgacaaatt cgctatttat ttatgagagt tttcgtacta tattatatta atatgcattc
     2581 attaaggtta gtttgaagca gtttggtatt taaagtgtaa ttgaaagaga gtggggcgcc
     2641 ttatgtcatt cgtaacagaa aatccatggt taatggtact aactatattt atcattaacg
     2701 tttgttatgt aacgttttta acgatgcgaa caattttaac gttgaaaggt tatcgttata
     2761 ttgctgcatc agttagtttt ttagaagtat tagtttatat cgttggttta ggtttggtta
     2821 tgtctaattt agaccatatt caaaatatta ttgcctacgc atttggtttt tcaataggta
     2881 tcattgttgg tatgaaaata gaagaaaaac tggcattagg ttatacagtt gtaaatgtaa
     2941 cttcagcaga atatgagtta gatttaccga atgaacttcg aaatttagga tatggcgtta
     3001 cgcactatgc tgcgtttggt agagatggta gtcgtatggt gatgcaaatt ttaacaccaa
     3061 gaaaatatga acgtaaattg atggatacga taaaaaattt agatccgaaa gcatttatca
     3121 ttgcgtatga acctcgaaac atacatggtg gattctggac taaaggcatt cgtcgtagaa
     3181 agcttaaaga ttatgaacca gaagaactgg aaagtgtagt agaacatgaa attcaaagta
     3241 aatgagaatg aaacaattgc tgattgtttg tcacgaatga aaatgcaagg gtatatgccg
     3301 gtaaaacgta ttgaaaaacc cgtgtttcaa gagcaaaaag atggcacggt tgaagtatca
     3361 catcaagaaa tcatttttgt aggtaagaaa atccaataac ataatccaat ttaaataaag
     3421 actatttgaa gaggaaaggc tattcaaagt ttgagtaatt ttactttgaa tagcctattt
     3481 gtttatacat gcaagatgct cgatccatat tgtatgagaa acccccagca agctatataa
     3541 agcatatgct gggggttctt aatattttaa aaattattgt tagattatat atatcgtcgc
     3601 tttttctaaa aaaatctcat cgcatgaaat tttttcttcc tagagacctt taataagatt
     3661 aatagtttac ttaatcatat ctagatagtc ttatgacttt tgcttaatga aagtcattct
     3721 aggagaagtt cccaaagct
//
LOCUS       DAMZLE010000186         3710 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMZLE010000186 DAMZLE010000000
VERSION     DAMZLE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.190"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            138..488
                     /locus_tag="POB92_002527"
     CDS             138..488
                     /locus_tag="POB92_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDI6579986.1"
                     /translation="MNDLVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            481..1044
                     /locus_tag="POB92_002528"
     CDS             481..1044
                     /locus_tag="POB92_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDI6579987.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1058..1402
                     /gene="gpsB"
                     /locus_tag="POB92_002529"
     CDS             1058..1402
                     /gene="gpsB"
                     /locus_tag="POB92_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDI6579988.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1465..1844
                     /gene="rnpB"
                     /locus_tag="POB92_002530"
     ncRNA           1465..1844
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="POB92_002530"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2053..3198
                     /locus_tag="POB92_002531"
     CDS             2053..3198
                     /locus_tag="POB92_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDI6579989.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            3282..3614
                     /locus_tag="POB92_002532"
     CDS             3282..3614
                     /locus_tag="POB92_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDI6579990.1"
                     /translation="MGKKMGLGLSIALIVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT         1388 a          484 c          712 g         1126 t
ORIGIN      
        1 ttatatagga agtttttaga tttacttgat ttaacagata acacatttga aaaaatagtt
       61 aaatcaaatt atatagagtg ttaaaacatg acgaatacag tatattgtga taaattaaaa
      121 atgtaggagg cgtcctcatg aatgatttag tcgaatcact aatttatgaa gttaacaaca
      181 tgcaacaaaa ttttgaaaat gtgaaatcac aacaacaaga tcatgatttt taccaaactg
      241 taaagccata tactgaacat attgatagca tgctcaatga gatcaaatta catcgtgaat
      301 ttattataga agtaccttat atgaattcaa ggaaatttga gctactgatt gctaacattg
      361 aacaactttc tgtcgaatgt cattttaagc gaacaagtcg aaagttattt atagaaaagc
      421 ttaaaagtgt tcaatatgat ttacaaaata tattagatgg cgtaacaaaa gagggtactg
      481 atggttaaaa cagtttatgt aacaggttac aaatcattcg aattaaacat ttttaaagat
      541 gacgcacctg aagtacatta tttaaaacaa tttataaaac ataaaattga acaactgttg
      601 gatgaaggat tagaatgggt gttaatacaa gggcaaatgg gtatagaatt atggactgca
      661 gaagttgtta ttgaattaca acgaacatat gattcgttaa agtttgctgt aattacaccg
      721 ttccaaggac atacagaaaa gtggaatgaa cataatcaaa gtaagtatgc caacataatt
      781 aagcatgcag attatgttga tagtattttt catacgtcgt atcagggtcc ttttcaattt
      841 aaacaagcag atcaatttat gcttgaacat tcggatcaaa cattgctcat ttatgatgag
      901 gaacaagagg caagtcctaa gttcttcaag cagatgttag ttgattttat ggataaaaca
      961 aactatactt gtgatattgt gacgttcgat gaattaacag cattcatcaa tgacttacag
     1021 tggtctgaag atcaaagttt ctaatgaggt ggaaaaaatg tcagatgttt cattgaaatt
     1081 atcagcaaaa gatatttatg aaaaagattt tgaaaaaacg atggctcgtg gctatagaag
     1141 agaagaagta gatgcatttt tagatgacat tattgctgat tatcaaaaaa tggccgatat
     1201 gaataatgaa gttgtaaaat tatcagaaga gaatcataaa cttaaaaaag aattagaaga
     1261 attaagacta cgtgtagcaa catcaagacc tcaggacaat aaaagttttt cttcgaataa
     1321 tacaacaaca aatacatctt caaataatgt agatatttta aaacgtattt caaacttaga
     1381 aaaagctgta tttggtaaat aattactaaa tacaaaagtt taactgtctt actaataatg
     1441 actatgttat aattttaaaa gtgatatttt gggtaatcgc tatattatat agaggaaagt
     1501 ccatgctcac acagtctgag atgattgtag tgttcgtgct tgatgaaaca ataaatcaag
     1561 gcattaattt gacggcaatg aaatatccta agtctttcga tatggataga gtaatttgaa
     1621 agtgccacag tgacgtagct tttatagaaa tataaaaggt ggaacgcggt aaacccctcg
     1681 agtgagcaat ccaaatttgg taggagcact tgtttaacgg aattcaacgt ataaacgaga
     1741 cacacttcgc gaaatgaagt ggtgtagaca gatggttatc acctgagtac cagtgtgact
     1801 agtgcacgtg atgagtacga tggaacagaa catggcttat agaaatatca ctactagttt
     1861 agctctccta gatgatggag agcttttttc atgaaaagaa cacttaaaat taacgccttg
     1921 tcttgatata atgacactgc cttgttttaa aatagtaagc ggatgcgtta atgtatcagc
     1981 gattaaattt gttggaaatg tataaaaaac acaagctaag aataaaatac ctgtataaaa
     2041 ggagaatcat atatgtttca attacttgca gtttgtccga tgggattaga agctgttgtt
     2101 gctagggaaa ttcaagaatt aggctatgaa acaaatgttg aaaatggtcg tatatttttt
     2161 gaaggagacg caagtgcaat tgtaaaggca aatttatggt tgcgcacagc agaccgaatc
     2221 aaaattgttg ttggacgttt taacgcaaca acgtttgacg aattattcga acaaaccaaa
     2281 gcgctccctt gggaatctat aattgataaa gaaggtaact tcccagttca aggtagaagc
     2341 gttaaatcaa cactacatag tgtgccagat tgtcaggcga taactaagaa agctattgtt
     2401 gaacgattac gtcgcgcata taacgaaaaa ggttggttaa atgaatcagg tgccaaatac
     2461 cctgttgaag ttgccatttt aaaagataat gtattattga ctatagacac atcaggttct
     2521 ggtttgaaca gacgtggata tagattagca caaggtgaag caccaattaa agaaacgttg
     2581 gcagcaagtt taatccgtct tgcaaactgg aaaggtgata cacctttaat tgatccattt
     2641 tgcggttcgg gtacaatagc tatagaagct tgtttaattg ctcaaaatat cgcacctggt
     2701 tttaatcgcg agttcgtatc agagcaatgg aacatcatgc cagcaaatat ttatgatgat
     2761 taccgtgatg aagcggataa gatggctgat tatgataaag aaatcgaagt atatgcttct
     2821 gatattgatc cagaaatggt agagattgct aagcgtaacg ctgaagaagt tgggttgtct
     2881 gatattatta aatttagtgt aaaagatgtc aatacattaa caattgatac agaagaaccg
     2941 gtggcgttaa ttggaaatcc tccatatggt gaacgtattg gtgatcgtga agaagttgaa
     3001 gaaatgtacc gttatattgg taaactaatg aaacaacatc catttttatc tacatacatt
     3061 ttaacaagta ataaagaatt tgaatattta gtagatcgta aagcaacgaa gcgacgtaag
     3121 ttatttaatg gatatataga atgtacttat tatcaatact ggggtaaaaa gacagaacgt
     3181 aaaactatcg aaaactaaag tgtttctaat gcgtgactaa aattagtatt aattatgttc
     3241 tcatgataat aggtgttttt gaaaaatgga ggagtctata aatgggtaaa aaaatgggtc
     3301 taggtttatc tatagcattg attgttattg gtattgccgt tgtatgttta atgatttttt
     3361 ctagtcaaaa aacgacttat tttggttata tgaatagtaa tacaaatgca gaaaaagttg
     3421 taagtgaaaa agatggatta gtcaaacata atatcaaagt agaaccatct aatgatttca
     3481 agccgaaaaa aggagacttt gtaaaattag tttctaaaga tgatgggaag acattttata
     3541 aacaagagat tgttaaacat gatgacgtcc cacacggttt aatgatgaaa attcacgaca
     3601 tgcatatgaa ttaataaaaa agcatctata acgtaatttt gaagaagtag agttatcttc
     3661 ttatgcgttt tagatgcttt tttatcttca aattcaaaaa atcggacaga
//
LOCUS       DAMZLE010000187         3683 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMZLE010000187 DAMZLE010000000
VERSION     DAMZLE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.191"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            136..1077
                     /locus_tag="POB92_002533"
     CDS             136..1077
                     /locus_tag="POB92_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6579991.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            1198..1752
                     /locus_tag="POB92_002534"
     CDS             1198..1752
                     /locus_tag="POB92_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDI6579992.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            1829..2599
                     /locus_tag="POB92_002535"
     CDS             1829..2599
                     /locus_tag="POB92_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6579993.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIAEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(2771..3568)
                     /gene="fdhD"
                     /locus_tag="POB92_002536"
     CDS             complement(2771..3568)
                     /gene="fdhD"
                     /locus_tag="POB92_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDI6579994.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
BASE COUNT         1255 a          529 c          673 g         1226 t
ORIGIN      
        1 agggaatggc tatcaataaa agaagaggtt taaaaggatt acaacagaat gttatgattt
       61 tgtagaaaga tatataacaa cgttttataa aacataatat tgttaatgga aaatgaaatg
      121 taagggggat ttcgagtgac taagaaagtt tattttaacc atgatggtgg tgtagatgat
      181 ttagtgtctc tatttttatt attacaaatg gaaaacgttc aattgatagg ggtcagtaca
      241 attggtgctg attgttattt agagccatct ttgagcgcat cagtaaaaat tattaatcgt
      301 ttttcaaatg aagatattca agttgcgcca tcatatgaac gaggaaaaaa tccatttcct
      361 aaagaatggc gtatgcatgc cttttttatg gacgcattgc caattttaaa tgagccagtc
      421 aaacatgttg cttcaaatgt gagcgacaaa gaagcctttg aagacattat tcaaacttta
      481 aagagacaat cagaaaaagt aacattatta tttacaggcc cgcttacaga tttagcaaaa
      541 gcactacaaa aagattcatc tatcgttcag tatatagaaa aattagtttg gatgggtggt
      601 acctttttac caaaaggaaa tgttgaagaa cctgagcatg atggttctgc agaatggaat
      661 gcatattggg atccagaagc ggttaaaatt gtttttgata gcgatataga gattgatatg
      721 gttgctttag aaagtacgaa tcaagtaccg ctaacgttag atgttagaca aagatgggcg
      781 aatgaacgcc aatatacggg cattgatttt ttaggagtaa gttatgcagc ggtaccacca
      841 ttaacacact ttataacaaa ttctacttac tttttatggg atgttttaac gactgcttat
      901 attggtaaca aggacttggt tcattcaatt gagaaaaaag tcgatgtaat aagttatgga
      961 ccaagtcaag gtaagacatt tgagtgtaaa gatgggcgca aaattaatgt cataaatcat
     1021 gtagataaca acgcattttt tgattatata actgcacttg ctaaaaaagt aaattaacag
     1081 ctgtgtagaa taattaaggt tttaatttat atagaacaac ttattgtaaa cttttcgttt
     1141 cttaaagttt acaatggtgc tataataatg gtcatgaaat acgaaaggaa gtaaaaaatg
     1201 acaacaaaac agttagtata tacagcttta atgacagcga ttatcgctat tttaggattg
     1261 gtaccggtaa ttccactacc attttcttca gtaccaattg tacttcaaaa cattggtatt
     1321 ttcttagcag gtgtgatttt aggacgtaaa tatggcacat taagtgttat cgtcttttta
     1381 ttattagtag ttgctggctt gccactgtta tcaggcggtc gcggtggcat tggtgtattc
     1441 gcaggtcctt cagcagggtt tttactatta tatccagttg tagcattcat gattggtgcg
     1501 attcgtgata gattcatcaa tgaaattaat ttctggattt tattcgttgg tattttagtt
     1561 tttggtgtta tagcattaga tgttattggt acattgatta tgggcatgat tattaacata
     1621 ccatttacga aagctatttc aatttcatta gcttatttgc ctggtgatat attaaaagca
     1681 attgtagcaa gtttgattgg tacagcttta cttaatcact cgcagtttcg tcaaattatg
     1741 ggattaaaat aatcatattt aagttaagat aataaagtaa ttgaataagt tgctttgaaa
     1801 tttataaaag tgaaaggagt aggtgtcaat ggctagtata agtatgtcag atatatattg
     1861 taacggcact atatttgaaa atgacgacga gcagttaatt tatttaacgc cttcttttcc
     1921 acaacgatac acaagcaaca catggatata taaaaagacg cctacccaag agcgatggct
     1981 gaaagactta gaacgtcaac atcaattaca tacaaatcaa ggttcaaatc attatgcgtt
     2041 tagtttcccg gaaaatgaac aacttgataa tcattggatg gctatgttta aagatatgaa
     2101 ttttgaacta ggtattatgg aattgtatgc catagaaagt gatgcgcttg ccaatttgcc
     2161 gcgtaactct gacgttgaaa ttgccatcgt tgacgagtcg catatagatg cctatttaaa
     2221 agttgcatat cagtttagtt tgccatttgg aaaagactat gcagatgcac atgaagaaat
     2281 ggtaagggaa cattatcaaa aagatgtgat taaacgctta gtagcttatt taaataatga
     2341 acctattggc gttgtagatg tcattgaaag tgaaaattac attgaattag atggatttgg
     2401 tgtattagaa caatttcggc accaaggaat tggatctaca attcaatcgt tgatagctga
     2461 atacgccata tcaaaaaatc acaaaccaat catattagtt gcagatggtg aagatacagc
     2521 aaaagatatg tatgcaaagc aaggttatgt ttaccaatca ttttgttatc aaatattaaa
     2581 agaagatatt ggaaattaac gattgatgat aggtttgtat tagaataaaa aagcagtaag
     2641 ctggtttcga gtagaaatca acttactgct ttttaaattg ttttgagcta cttatactta
     2701 taaaaatagt gcttttatat tgttgattca tgtagaatat cgttcattat gacacactat
     2761 aatgaatatg ttattgttca gagtcgatga tacgttctgg atgactatat atattaaagc
     2821 caccatttcg aataaatcct actgccgtaa tatttaggtc attagctaag gttacagcaa
     2881 gcgttgtcgg agctgattta gataaaatga cgccaacacc aatttttgcg gctttaatta
     2941 aaatttctga tgaaatacgt ccactaaaaa ttaatacttt atctcggaca gtaatatgtc
     3001 gctgaataca aaatccatat aatttatcta gagcgttatg tctaccaatg tcttgtcgat
     3061 gtacaaaaaa tgtcaaacca tcgcttatag cagcattatg taagccacct gtttcttggt
     3121 aaatatgact tgcactttgt aatcgagtca tcatgttaat aatttgcatt ggagttaaag
     3181 tgattttaga catagatgtt ttagcgatag cagcatcatt ttgaaaataa aactcacgac
     3241 tctttccaca acaagatgca atcattcgtt ttgtggaata ttgaaagcga tcgcctaaat
     3301 ctttattaag ttcaacatgg gcaaaacctt tactatcatc aatcagcaca gattttaatt
     3361 catctcgctt taaaatggca ccttccgaag ccagaaatcc aatgactaac tcctcaaggt
     3421 ttgttggact gcatataaca gtcgcaaatt cttcaccatt caccataatt gtaagtggaa
     3481 attctgtcac atattgatct gttgtattga ataattttcc atcttcatat ctaacaattg
     3541 gttgacctaa agatacatct ttgttcatta tctaacccct ttaattagct taaactttat
     3601 tttaaagcaa tttgcttaaa attttaacat atttgcttaa gtttgaaatt tgatttataa
     3661 aaaataatag cgagcaatct gtt
//
LOCUS       DAMZLE010000188         3660 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMZLE010000188 DAMZLE010000000
VERSION     DAMZLE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.192"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            113..1492
                     /locus_tag="POB92_002537"
     CDS             113..1492
                     /locus_tag="POB92_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDI6579995.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(1612..2640)
                     /locus_tag="POB92_002538"
     CDS             complement(1612..2640)
                     /locus_tag="POB92_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDI6579996.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     LRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            2910..3311
                     /locus_tag="POB92_002539"
     CDS             2910..3311
                     /locus_tag="POB92_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDI6579997.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(3329..3537)
                     /locus_tag="POB92_002540"
                     /pseudo
     CDS             complement(3329..3537)
                     /locus_tag="POB92_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1255 a          621 c          553 g         1231 t
ORIGIN      
        1 tttcaacttt caacatatat aattcgaaag accatttaaa attaacggcc acaacattca
       61 aatcaattaa tcgctttttc caaaataatc atataaggag gttcttttca ttatgaatat
      121 cattgagcaa aaattttatg acagtaaagc ttttttcaat acacaacaaa ctaaagatat
      181 tagttttaga aaagagcaat taaagaagtt aagcaaagct attaaatcat acgagagcga
      241 tattttagaa gcactatata cagatttagg aaaaaataaa gtcgaagctt atgctactga
      301 aattggcata actttgaaaa gtatcaaaat tgcccgtaag gaacttaaaa actggactaa
      361 aacaaaaaat gtagacacac ctttatattt atttccaaca aaaagctata tcaaaaaaga
      421 accttatgga acagttttga tcattgcacc atttaactat ccttttcaac tagtgttcga
      481 acctttaatc ggtgctattg cagcaggtaa tacggcaatt attaaaccat ctgagttgac
      541 accaaatgtt gcacgagtga ttaaacgatt aatcaatgaa acatttgatg caaattacat
      601 tgaagttatt gagggaggaa ttgaagaaac gcaaacgtta attcacttac cttttgacta
      661 tgtcttcttt acaggaagtg aaaatgtagg caaaatcgtt tatcaagctg ccagcgaaaa
      721 tttagttcct gtgacattag aaatgggtgg taaatctcca gtcatcgttg atgaaacagc
      781 gaatattaaa gttgctagtg agcgcatttg ttttgggaaa ttcactaatg ccggccaaac
      841 atgtgttgca ccagattaca ttttagtaca cgaatctgta aaagatgatt taatcacagc
      901 cctatcaaaa acgttgcgtg aattttatgg tcaaaatata caacaaagtc cagattatgg
      961 ccgcattgta aaccttaaac attatcatcg tctgacttca ttacttaaca gtgcacaaat
     1021 gaatattgta tttggtggtc atagtgatga ggatgaacgt tatatagaac caacattgtt
     1081 agatcacgtt acaagtgatt cagcaattat gcaagaagaa atttttggtc ctatcttacc
     1141 gattttaacg tatcagtcat tggatgaagc aatagccttt attcaccaaa gaccaaaacc
     1201 tttgagttta tatttattta gcgaagatga aaatgctaca caacgtgtaa taaacgagct
     1261 atcatttggc ggcggcgcta ttaatgatac attgatgcac ctagcgaatc ctaaattacc
     1321 atttggtggt gttggcgcct caggtatggg acgctatcat ggtaaatatt cattcgacac
     1381 ttttacacat gaaaaaagct acattttcaa atctacacga ttagaatcag gtgtccattt
     1441 accaccatat aaaggtaaat ttaaatacat caaagctttc tttaaaaatt aatttttgag
     1501 ccaaaaataa accatgcttc cataatttga tgttcttgaa aacaataaat ttcaaggtat
     1561 acacaaattt tttggaaaca tggttttctt tttatcatga ttaattaaag attaaaatat
     1621 gacacataca ccttcagatg ctacacgagt gttatcacat agcgtaattt tacctgtttc
     1681 tttatttctt tcgaaaacag ttacaactga atctccttgc tcatgagcac aaactaaaag
     1741 gtcatctgat gaggcaatat taaaatctct tgggaattga ccaccacttt cagtaattgt
     1801 tactagttct aagtgttgac cattatcaag aactttaaaa attgcaatgc tatcatgccc
     1861 tctattagat acatataaga attgttgatc atgagataaa cgcactgctg caagtttagt
     1921 atctccatca aagttttcag gaattgttaa atgacgctcg agctcttcaa atttaccgtc
     1981 attatattct gcaacactca cagtatttga taattcgtgt acgacataag caaattttcc
     2041 attatcatga aattcaatat gtcttgtccc atcactatct ttaaataaag attctttata
     2101 aaattcaaac ccgttgtcat caaatttata agtaacgata cgatcagcac ctaaatctgt
     2161 tactgcaacg tacttaccat ctggagtttg attaatataa tgtgcgtgtg gatgatcttg
     2221 tctttcatgt gtacctgttg gaaaatcatg cgcaagttct tgaattagac gtataatttc
     2281 acctgtgtgc gtgtttaatt catacatacg tatgatgcca gcaccatata ccgcttcaaa
     2341 taaatatcgt ttatcttctg aaatcgatac ataacaacct gtaccagctt ttgaagacaa
     2401 acatttattt aataaatgca attcaccatt gtcatcaatt ttcaaactcg caacaccgca
     2461 ttgttctcct tctttgttga ttccatataa aacttcatta ttacgcaaca aatatgtaga
     2521 cgcttctaat tcaaatcctg tttctaataa atcaatacgt gactgatttt cgtttaattc
     2581 aaagcgataa atccctttac cattcttttt agtgtaagaa ccaatatatc catttgtcat
     2641 tacttttacc cctccatgtt agtcttattt tcattttatt aaattttagc tagaattaac
     2701 aatcatttat catttcctta aagctaaaac tatttaacta ctttaaaaga actatttgct
     2761 gtgatatgtc attattcgat tttaattaat ccctcttttt tacttacacg agcattaatt
     2821 attaacgcgt ttatgcttcc acaaaatttt tgttgcaaaa tagaacacat gttcgtataa
     2881 tgtaattatc agattggggt gaattaagga tgacaagtta tgaagaagaa actgattatc
     2941 gtaacgttcc aagagaatta ctagataaca atatacctat gggaagaggc atgattaaat
     3001 gggctccttt tgcaacatta cccgaacaat tcgaaacgat tcaacaatac attatcgacc
     3061 aaaacaaaat tacacgtcct gtattatcag atgatcaatt ggctgagcta aacattcggc
     3121 tacacgaggc gctacaatat gcccaacctg tagaagttaa attttataat aatggctttg
     3181 tagattcagt acgcttaacc atttatcgta ttgatgctat taattatgaa attgatggtt
     3241 atgtttacaa tcagcaacaa cgtcaaaaaa tttctatctt tgatatctta gatattgtct
     3301 tgttacctta atgctttgaa cataattaaa cagattgagt atatgcatat aagatgattt
     3361 ttaacatcat ttttggatga taggatgttg cgccacgatg atgtctgaat tcatcgaatt
     3421 cgctatcagg tattgtttca acaatttcat taacatatcg tgaaatatca ttttgaggga
     3481 ttctaacaga agtttctatt ggtagtgtag ttgggtcatg ttataatttt tatacataag
     3541 gcacctcgtt aatttagttt agtggtattt attaaattat aagaagggac ccaacacaga
     3601 aaattcattt tattgaattt tacatttatg tgcaagttgg gcaaagtgtc ttatttttta
//
LOCUS       DAMZLE010000189         3630 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMZLE010000189 DAMZLE010000000
VERSION     DAMZLE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.193"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..428)
                     /locus_tag="POB92_002541"
     CDS             complement(<1..428)
                     /locus_tag="POB92_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6579998.1"
                     /translation="MNEQIIGSIYTLAGGVVLYSVKEIFRYFTDSNLQRKKINLEQIY
                     PIYLDCFKKAKKMIGAYIIPTEQHEFLDFFDIEVFNNLDKQSKKAYENVIGFRQMINL
                     SNRVKAMEDFKMSFNNEFSTNQIFFNPSFVMETIAIINEY"
     gene            complement(587..736)
                     /locus_tag="POB92_002542"
     CDS             complement(587..736)
                     /locus_tag="POB92_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6579999.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            complement(733..828)
                     /locus_tag="POB92_002543"
                     /pseudo
     CDS             complement(733..828)
                     /locus_tag="POB92_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
     gene            complement(813..1064)
                     /locus_tag="POB92_002544"
                     /pseudo
     CDS             complement(813..1064)
                     /locus_tag="POB92_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1077..1481)
                     /locus_tag="POB92_002545"
     CDS             complement(1077..1481)
                     /locus_tag="POB92_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDI6580000.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYVHTYMPTNYTKHKKYL
                     QSQMPRLNLENALKIELAFYFTPPESWSKKKKAQAIGQLKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            complement(1490..1708)
                     /locus_tag="POB92_002546"
     CDS             complement(1490..1708)
                     /locus_tag="POB92_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580001.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGIKETKFNGRRW
                     YLDDYELNTLIDNEQMELGHQTNLFEYI"
     gene            complement(1715..2608)
                     /locus_tag="POB92_002547"
     CDS             complement(1715..2608)
                     /locus_tag="POB92_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDI6580002.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KAAAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQIESKKQTYKPHVKQSKEKTPKWLTDGTRETKTAE
                     VDENLEKDREAFIKRLNSKWE"
     gene            complement(2638..3108)
                     /gene="ssb"
                     /locus_tag="POB92_002548"
     CDS             complement(2638..3108)
                     /gene="ssb"
                     /locus_tag="POB92_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6580003.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNTDDNQQDLYQQQAQQTRGQSQYSNNKPVKDNPFANANCPIEIDDNDLPF"
     gene            complement(3109..3594)
                     /locus_tag="POB92_002549"
     CDS             complement(3109..3594)
                     /locus_tag="POB92_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6580004.1"
                     /translation="MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEP
                     VAFLLQSTLGYKILYVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSALAN
                     RIMESHFSLEHAIGMLKANDLTRLEEIHLIHLSSQNSNAKYIKREIQKVTGVPVYVGG
                     L"
BASE COUNT          984 a          697 c          520 g         1429 t
ORIGIN      
        1 tgatattcat ttataatagc aattgtttcc ataacaaaag aaggattaaa aaaaatctga
       61 tttgtactaa attcattgtt gaaactcatc ttaaaatctt ccattgcctt aactctattt
      121 gataaattaa tcatttgtct aaatccaata acattttcat acgctttttt actttgctta
      181 tctaaattat taaagacttc aatatcaaaa aaatctaaaa attcatgctg ttctgttgga
      241 ataatataag ctccaatcat ctttttagcc tttttaaaac aatctaaata tatcggatat
      301 atttgttcta aattgatttt tttacgttgt aagttagaat ctgtaaaata cctaaaaatc
      361 tctttaactg aataaagcac aacacctcct gctaaagtat atatgcttcc tattatttgc
      421 tcgttcattt ttatctacct ctttataata ttttctgaaa aggaatctat aattttatac
      481 ataccaaaaa ttcctaatgc atttattatc actccatcat caataatata tatggatatt
      541 aagaaagcaa acagcaaaac gatcaaatca taaataaata ttctcattat tcactcacct
      601 ccgctcgaaa gacgtaatca ctcggcgcct ctacatcatc attagccgtc atcataatat
      661 atacttgctc agttacatac ttacctaact cgtacattgc tagtaggaat aataatctta
      721 gtatttgctt aatcatttcc cacactccct tatattttca aacaactgac ccactttaat
      781 aactgcacct cttttaactt gcgcctcgta cctctctttc gctttctttc caatttgcgt
      841 tcgagtctag cttgttccag tctttctatt gttttctttt ctctgtactc gcttaaacgc
      901 gtaccttctg gtgcgtccat tgcttcatgt agttcccaac cgtcttttac tctcttagaa
      961 accattccag cggttatacc gtgactttct attaattcca tttcaaattt actgaaccta
     1021 taaggtttat catttattgt tacaatcctt gcttttctcg ccattttatc cacctcttat
     1081 atttcttcta ttcgtatgat tattttgggc tcaattccat aacgctttga gctagttatt
     1141 tctgtaattt ggttatcgtc tttccataca tggccattac aagcatctaa taccgtttta
     1201 attaagttgt cgatatccgg cttagtcact tttaattgtc caatcgcttg agctttcttt
     1261 ttcttcgacc atgattcagg tggagtaaag taaaacgcta attcgatttt taatgcattt
     1321 tctagattta gccttggcat ttgactttgt aaatattttt tatgttttgt atagtttgta
     1381 ggcatgtatg tgtgtacgta tctgcctttt gtgctaaaac gcggtcgagg cgaccccatc
     1441 ggcgcattaa acacttcgtt aaatttaatt tctatctcca tgcaatccct catatatatt
     1501 caaataagtt tgtttggtgt cctaactcca tttgttcatt atcaataagt gtatttaatt
     1561 cataatcgtc taaataccaa cgacgaccat taaattttgt ttcttttatt ccaacaacta
     1621 aatgccgacc atctttaaaa tgtggtgtaa ctgaaaacat tttgttgccg tcatgatcaa
     1681 atagatagta tttatcaaat gcatccattt tcaatcactc ccatttgcta tttagacgct
     1741 taataaaagc ttctctgtct ttctcaaggt tttcatctac ttccgccgtt ttcgtttctc
     1801 tcgtgccgtc tgtgagccat ttaggcgttt tttcttttga ttgtttaaca tgaggtttgt
     1861 atgtttgctt tttgctttca atttgttgct tttcaaatgc acgtacttgt tcgatagatt
     1921 tcaagtttgc attaagccat gtattcaaaa tgcttttagc atatccccaa gtaactttgt
     1981 ttctgtcttt agcgatttta agtgatgccg taactatttc atctgaatca ttttcaaatg
     2041 aatcaagata ataatttaaa tcgtctaaat tgtatggagt tatgaaaccg aatccgttat
     2101 cttggaagaa gtcgaaggca gctgccttct tcttctcatt attcacattc ttttcattat
     2161 tatctttatt atcattattg tttgtgttgg tttgatgttg ttttgatgtt ggattgatgt
     2221 ttgactgatg ttgttttgat gttggtttga tgtcgttttg atgttggttc cttccctgct
     2281 cactttgata aaagtcataa ttgacaatgg ttataagggt atattttgat gttgttttga
     2341 cttctaacat tccatcactc tcgagtaagt caaggaaggt tttcacttta aatcgtgacc
     2401 agttaaaaag gtcagacaag gtcaaaatcg atgttaatct ttgtcctctt tctacggtta
     2461 caatttggtt tccaataggc acttttgcct ttgaatgatt cgcttccatg agtaaatata
     2521 tccatgcttc aaactttgaa aatgttctct tttctttaaa tagccaatga ttttgaattg
     2581 agcgatcaat acttatccaa ccagtcatat acacacctca ctttcaaacc ggttaaatta
     2641 gaatggtaaa tcattgtcat ctatttcaat cggacaattt gcattcgcaa acggattatc
     2701 ttttactggt ttgttatttg aatattgcga ttgtccacgt gtttgttgcg cttgttgttg
     2761 gtataaatct tgttgattat catctgtgtt cttcggttct aaaaattgaa tactatcggc
     2821 aacaacttcc gtaacgtata cacgttgacc ttccttattt tcatagttcc gcgtttgtaa
     2881 cctaccatct acgcccgcca acgatccttt agataggtat ttattaacgt tctctgcttg
     2941 ttttttaaat acgatgatat taataaagtc tgcctcgcgc tctccttgtg cattcgtaaa
     3001 tgtgcggttt actgctaatg tgaatgatgc tacatttaca ccactttgag tggttcttaa
     3061 ttctgggtct ctagttaaac gaccaactaa tattgttctg tttagcattt ataaacctcc
     3121 aacataaacg ggcacgcccg tcactttttg tatttctctt ttaatgtatt ttgcatttga
     3181 attttggcta cttaaatgaa ttaaatgtat ttcttcgagt ctagttaaat catttgcttt
     3241 caacattcct atagcatgtt ctaagctaaa atgagactcc ataattctat ttgctaatgc
     3301 gctgtgtaca ctgccgtttt ttatgttttc ttgcatttgt tcatagatat aattaacttc
     3361 taacatcatg tgtgtaatgc cgttaaattt gtatttcaga tacttcgtat cagtaacata
     3421 caggatctta taacctagtg tgctttgtaa taagaaagcc acaggctcgt tagcatcatg
     3481 ttcgatgtca aacggtagaa ttgaccatgt acctattcgc agctcttgct ttgccttaat
     3541 cgtgcataag cgatgacttt caaaattcat agcttgttgt gttccagcag tcatatagct
     3601 gattacacca ttgtcgacaa actgctttgt
//
LOCUS       DAMZLE010000190         3625 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMZLE010000190 DAMZLE010000000
VERSION     DAMZLE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.194"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            54..3605
                     /gene="cna"
                     /locus_tag="POB92_002550"
     CDS             54..3605
                     /gene="cna"
                     /locus_tag="POB92_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen adhesin Cna"
                     /protein_id="HDI6580005.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNEALAARDISSTNVTDLTVS
                     PTKIEDGGKTTVKMTFDDKSVKIQNGDTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSNYYSGSNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSYNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIITDANGIANIKALPSGDYILKE
                     IEAPAPYTFDKDKEYPFTMKDTDNQGYFTTIENAKEIEKTKDVSAQKVWEGTQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKTIKYLVKEVNAQG
                     EDTTPEGYTKKENGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGE
                     KVKTLDVTSETNWKYEFKNLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGE
                     TSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWTGLDEKAK
                     GQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTSISGEKVWDDKDNQDGKR
                     PEKVSVNLLANGEKVKAVDVTSETNWKYEFKDLPKYDEGKKIEYTVTEDHVKDYTTDI
                     NGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTAKLNESNN
                     WTHTWTGLDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTSINGE
                     KVWDDKDNQDGKRPEKVSINLLANGEKVKTLDVTSETNWKYEFKDLPKYDEGKKIEYT
                     VTEDHVKDYTTDINGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQDGK
                     ATGKTATLNESNNWTHTWAGLDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVT
                     NKYTPEKPNKPIYPEKPKDKTPPTKPDHSNKVKPTPPDKPSKVDKDDQPKDNKTKPEN
                     PLKELPKTGMKIITSWITWVFIGILGLYLILRKRFNS"
BASE COUNT         1597 a          517 c          705 g          806 t
ORIGIN      
        1 aatttatcat tagtatccta tggatatgac atagagtata aggaggggtt tttatgaaca
       61 agaacgtgtt gaagtttatg gtctttataa tgttattaaa tatcatcacg cctttattta
      121 ataaaaatga agcattggca gcacgagata tttcatcaac gaatgttaca gatttaactg
      181 tatcaccgac taagatagaa gatggtggta aaacgacagt aaaaatgaca ttcgacgata
      241 aaagtgtaaa aatacaaaat ggtgacacga ttaaagtggc atggccgaca agcggtacag
      301 taaagataga gggttatagt aaaacagtac cattaactgt taaaggtgaa caggtgggtc
      361 aagcagttat tacaccagac ggagcaacaa ttacattcaa tgataaagta gaaaaattaa
      421 gtgatgtttc gggatttgca gaatttgaag tacaaggaag aaatttaacg caaacaaata
      481 cttcagatga caaagtagct acgataacat ctgggaataa atcaacgaat gttacggttc
      541 ataaaagtga agcgggaaca agtagtgttt tctattataa aacgggagat atgctgccag
      601 aagatacgac acatgtacga tggtttttaa atattaataa tgaaaaaaga tatgtatcga
      661 aagatattac tataaaggat cagattcaag gtggacagca gttagattta agcacattaa
      721 acattaatgt gacaggtaca catagcaatt attatagtgg atcaaatgca attactgatt
      781 ttgaaaaagc ttttccaggt tctaagataa ctgttgataa tacgaagaac acaattgatg
      841 taacaattcc acaaggctat gggtcatata atagtttttc aattaactac aaaaccaaaa
      901 ttacgaatga acagcaaaaa gagtttgtta ataattcaca agcttggtat caagagcatg
      961 gtaaggaaga agtgaacggg aaatcattta atcatactgt gcacaatatt aatgctaatg
     1021 ccggtattga aggtactgta aaaggtgaat taaaagtttt aaaacaggat aaagatacca
     1081 aggctcctat agctaatgta aaatttaaac tttctaaaaa agatggatca gttgtaaagg
     1141 acaatcaaaa agaaattgag attataacag atgcaaacgg tattgctaat attaaagcgt
     1201 tgcctagtgg agactatatt ttaaaagaaa tagaggcgcc agcaccgtat acatttgata
     1261 aggataaaga atatccgttt actatgaaag atacagataa tcagggatat tttacgacta
     1321 ttgaaaatgc aaaagagata gaaaaaacaa aagatgtatc tgctcaaaag gtttgggaag
     1381 gcactcaaaa agtgaaacca acgatttatt tcaagttgta caaacaagat gacaatcaaa
     1441 acacaacacc agtagacaaa gcagagatta aaaaattaga agatggaacg acaaaagtga
     1501 catggtctaa tcttccagaa aatgacaaaa atggcaagac tattaaatat ttagttaaag
     1561 aagtaaatgc tcaaggtgaa gacacaacac cagaaggata tactaaaaaa gaaaatggtt
     1621 tagtggttac taatactgaa aaaccaatcg aaacaacatc aattagcggt gaaaaagtat
     1681 gggacgacaa agacaatcaa gatggtaaga gaccagaaaa agtcagtgtg aatttattgg
     1741 ctaatggaga gaaagtaaaa acgttagacg tgacatctga aacaaactgg aagtacgaat
     1801 ttaaaaactt accgaagtat gatgaaggaa agaaaataga atatacagtg accgaagatc
     1861 acgtaaaaga ctacacaaca gacatcaacg gtacgacaat aacgaacaag tatacaccag
     1921 gagagacatc ggcaacagta acaaaaaatt gggatgacaa taataaccaa gacggaaaac
     1981 gaccaactga aattaaagtt gagttatatc aagatggaaa agcaacagga aaaacggcaa
     2041 cattaaatga atctaataac tggacccata cgtggacagg attagatgaa aaagcaaaag
     2101 gtcaacaagt aaaatacaca gtcgaggaat taacaaaagt taaaggctat acaacacatg
     2161 tggataacaa tgatatgggt aacttgattg tgacgaataa atatacgcca gaaacaacat
     2221 caattagcgg tgaaaaagta tgggacgaca aagacaatca agatggtaag agaccagaaa
     2281 aagtcagtgt gaatttatta gctaacggag agaaagtaaa agcggtagat gtgacatctg
     2341 aaacaaactg gaagtacgaa tttaaagact taccgaagta tgatgaagga aagaaaatag
     2401 aatatacagt gaccgaagat cacgtaaaag actacacaac agacatcaac ggtacaacaa
     2461 taacgaacaa gtatacacca ggagagacat cggcaacagt aacaaaaaat tgggatgaca
     2521 ataataacca agacggaaaa cgaccaactg aaattaaagt tgagttatat caagatggaa
     2581 aagcaacagg aaaaacggca aaattaaatg aatctaataa ctggacccat acgtggacag
     2641 gattagatga aaaagcaaaa ggtcaacaag taaaatacac agtcgaggaa ttaacaaagg
     2701 tcaaaggtta tacaacacat gtggataaca atgatatggg caacttgatt gtgacgaata
     2761 aatatacgcc agaaacaaca tcaattaacg gtgaaaaagt atgggacgac aaagacaatc
     2821 aagatggtaa gagaccagaa aaagtcagta taaatttatt ggctaatgga gagaaagtaa
     2881 aaacgttaga cgtgacatct gaaacaaact ggaagtacga atttaaagac ttaccgaagt
     2941 atgatgaagg aaagaaaata gaatatacag tgaccgaaga tcacgtaaaa gactacacaa
     3001 cagacatcaa cggtacaaca ataacgaaca agtatacacc aggagagaca tcggcaacag
     3061 taacaaaaaa ttgggatgat aataataacc aagacggaaa acgaccaact gaaattaaag
     3121 ttgagttata tcaagatgga aaagcaacag gaaaaacggc aacattaaat gaatctaata
     3181 actggaccca tacgtgggca ggattagatg aaaaagcaaa aggtcaacaa gtaaaataca
     3241 cagtcgagga attaacaaaa gttaaaggtt atacaacaca tgtggataac aatgatatgg
     3301 gtaacttgat tgtgacgaat aaatatacgc cagaaaaacc gaataaacca atctatcctg
     3361 aaaaaccaaa agacaaaaca ccaccaacta aacctgatca ctctaataaa gttaaaccaa
     3421 ctcccccaga taagccatca aaagtggata aggatgatca acctaaagat aataaaacca
     3481 aacctgaaaa tcctctaaaa gaattaccaa aaactggtat gaagattata acttcatgga
     3541 ttacatgggt atttataggt atattgggat tgtatttaat tttaagaaaa agatttaact
     3601 cataaaccat tataattatt tttat
//
LOCUS       DAMZLE010000191         3583 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMZLE010000191 DAMZLE010000000
VERSION     DAMZLE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.195"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..878
                     /gene="hlgA"
                     /locus_tag="POB92_002551"
     CDS             <1..878
                     /gene="hlgA"
                     /locus_tag="POB92_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDI6580006.1"
                     /translation="LANPFIEISKAENKIEDIGQGAEIIKRTQDITSKRLAITQNIQF
                     DFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNVDL
                     INYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQ
                     NSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARDYFVPDNQLPPLIQSGFNP
                     SFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAVDRKHDAFKNRNVTVKYEV
                     NWKTHEVKIKSITPK"
     gene            1446..2393
                     /gene="hlgC"
                     /locus_tag="POB92_002552"
     CDS             1446..2393
                     /gene="hlgC"
                     /locus_tag="POB92_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDI6580007.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDTLILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            2395..3372
                     /gene="hlgB"
                     /locus_tag="POB92_002553"
     CDS             2395..3372
                     /gene="hlgB"
                     /locus_tag="POB92_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDI6580008.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(3424..>3583)
                     /locus_tag="POB92_002554"
     CDS             complement(3424..>3583)
                     /locus_tag="POB92_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDI6580009.1"
                     /translation="LGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLR
                     KRMAVLLR"
BASE COUNT         1429 a          569 c          550 g         1035 t
ORIGIN      
        1 ctttagccaa tccatttata gaaatttcta aagcagaaaa taagatagaa gatatcggcc
       61 aaggtgcaga aatcatcaaa agaacacaag acattactag caaacgatta gctataactc
      121 aaaacattca atttgatttt gtaaaagata aaaaatataa caaagatgcc ctagttgtta
      181 agatgcaagg cttcatcagc tctagaacaa catattcaga cttaaaaaaa tatccatata
      241 ttaaaagaat gatatggcca tttcaatata atatcagttt gaaaacgaaa gactctaatg
      301 ttgatttaat caattatctt cctaaaaata aaattgattc agcagatgtt agtcagaaat
      361 taggctataa tatcggcgga aacttccaat cagcgccatc aatcggaggc agtggctcat
      421 tcaactactc taaaacaatt agttataatc aaaaaaacta tgttactgaa gtagaaagtc
      481 agaactctaa aggtgttaaa tggggagtta aagcaaattc attcgttaca ccgaatggtc
      541 aagtatctgc atatgatcaa tacttatttg cacaagaccc aactggtcca gcagcaagag
      601 actatttcgt cccagataat caattacctc ctttaattca aagtggcttt aatccatcat
      661 ttattacaac attgtcacac gaaagaggta aaggtgataa aagcgagttt gaaatcactt
      721 acggcagaaa catggatgct acatatgctt acgtgacaag acatcgttta gccgttgata
      781 gaaaacatga tgcttttaaa aaccgaaacg ttacagttaa atatgaagtg aactggaaaa
      841 cacatgaagt aaaaattaaa agcatcacac ctaagtaaac agttcaatca ttttataaaa
      901 tcctgggaca cttcatactt gtctcaggat tttttaacat attgaatcag cctcataaca
      961 ttaaattaat ttatcgtata ttaaatttaa taataacaac tgatttttat aagaacaaag
     1021 tatcacacca tagtagatac acaaataata caaatgaatc catttaagtt gaaagcttaa
     1081 ataaatattc tcaagttaat aaataattaa cttttagatg gattcatcaa aaatcgtaaa
     1141 aagaacaatt tgtattttac aaacattaat taaaaataaa agcaagacat tcgtgcaatc
     1201 ggttacctta aattgtttac aactgtcaac aataccaagg ttttattaac tatatttctc
     1261 acaaaattag cttttagcat tccaaacaaa aaaggttaaa ttgaacggaa ttatggcatt
     1321 tttaacttaa ttgtaaaaaa aagttgataa tggtcaattg ttaatgaaca gttaattata
     1381 ataacatcca aaatatatta ttatttaatt aagttaaata aaattataga aagaaagtga
     1441 aacttatgct taaaaataaa atattagcca caactttatc tgtgagctta cttgcccctc
     1501 ttgccaatcc gttattagaa aatgctaaag ctgccaacga tactgaagac atcggtaaag
     1561 gaagcgatat agaaattatc aaaaggacag aagataaaac aagtaataaa tggggcgtga
     1621 ctcaaaatat tcaatttgat tttgtgaagg ataaaaaata taacaaagat actttgatat
     1681 taaagatgca aggattcatt agctctagaa caacatatta caactataaa aaaactaatc
     1741 atgttaaagc tatgcgatgg ccattccaat ataatattgg tttaaaaaca aatgataaat
     1801 atgtttcttt aattaattat ttacctaaaa ataaaattga atctacaaac gtgagtcaga
     1861 cattaggata caatatcggt ggtaatttcc aatcagcccc atcactcggt ggtaatggat
     1921 catttaacta ttctaaatcg attagctata cacaacaaaa ttatgtaagt gaagtagaac
     1981 aacaaaactc aaaaagtgtt ttatggggcg tcaaagcgaa ttcattcgcc actgaatcag
     2041 gtcaaaaatc agcatttgat agcgatttat ttgtaggcta caaacctcat agtaaagatc
     2101 ctagagatta tttcgttcca gacagtgagt taccacctct tgtacaaagt ggatttaacc
     2161 cttcatttat cgccacagta tctcatgaaa aaggttcaag cgatacaagc gaatttgaaa
     2221 ttacttacgg aagaaacatg gatgtcactc atgccattaa aagatcaacg cattatggca
     2281 acagttattt agacggacat agagtccata atgcatttgt aaatagaaac tatactgtga
     2341 aatacgaggt caattggaag actcatgaaa tcaaggtgaa aggacagaat tgatatgaaa
     2401 atgaataaat tagtcaaatc atccgttgct acatctatgg cattattatt actttctggt
     2461 actgctaatg ctgaaggtaa aataacacca gtcagcgtaa aaaaagtcga tgacaaagtt
     2521 actttataca aaacaacagc cacagcagat tctgataaat ttaaaatttc acagatttta
     2581 acatttaatt tcatcaaaga taaaagttat gataaagata ctttagtact taaagctact
     2641 gggaatatta actcaggctt tgtgaaacct aatcctaatg actatgactt ttcaaaatta
     2701 tattggggag ctaaatacaa tgtatctata agctcacaat ctaatgattc agtaaacgtc
     2761 gttgattatg caccaaaaaa tcaaaatgaa gagtttcaag ttcaaaatac tttaggctat
     2821 acatttggtg gtgacattag tatctctaat ggtttatctg gtggacttaa tggaaataca
     2881 gctttttctg aaacaattaa ttataaacaa gaaagttaca gaacaacatt aagtcgcaac
     2941 acaaattata aaaatgttgg ctggggagtt gaagcacata aaattatgaa taatggttgg
     3001 ggaccttatg gacgagatag cttccaccca acatatggta atgaactctt cttagctggc
     3061 agacaaagca gtgcatacgc tggccaaaac ttcatagcgc aacaccaaat gccattatta
     3121 tctagaagta acttcaatcc agaattttta agcgtactat cacacagaca agatggcgct
     3181 aaaaaatcta aaattacagt aacttatcaa cgtgaaatgg atttatacca aattcgttgg
     3241 aatggcttct actgggcagg cgcaaattat aaaaacttta aaactagaac atttaaatca
     3301 acatatgaaa ttgattggga aaatcacaaa gtgaaattgt tagatacaaa agaaactgaa
     3361 aacaataaat agctagtaaa acacggtcgc caacagtaat tgtgacgacc gtgttttgat
     3421 ttattatctt agtaagactg ccattctttt tctcaatgtg agatataaag gaatagctac
     3481 aattaaagtg aatattacgc ctggaatcgc gtttaacaac actacccaca caggtaaatt
     3541 taaaataata gataatagta gcctagatac caaactgcct aat
//
LOCUS       DAMZLE010000192         3543 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMZLE010000192 DAMZLE010000000
VERSION     DAMZLE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.196"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            197..>3543
                     /locus_tag="POB92_002555"
     CDS             197..>3543
                     /locus_tag="POB92_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDI6580010.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNAQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLE
                     LKSQIKVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKEVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTTSESPTLVQMVTLSSNNSKSASMGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLD"
BASE COUNT         1500 a          486 c          607 g          950 t
ORIGIN      
        1 ttttatgata attaaataaa gattacaaaa ctcataaaaa cttttattta aacgcaaatg
       61 ttaaataacg ttttacgata aagaaaaata attaaagtat tgtgctttat ctaaaagtgt
      121 aatactatat tgatatacat ttttgtattt aaaacaattg tattatttaa aaatttgatg
      181 aattaggagt aatctaatgc taaacagaga aaataaaacg gcaataacaa gaaaaggcat
      241 ggtatccaat cgattaaata aattttcgat tagaaagtac acagtgggaa cagcatcaat
      301 tttagtaggt acaacattaa tttttggtct ggggaaccaa gaagcaaagg ctgcagaaag
      361 tactaataaa gaattgaacg aagcgacaac ttcagcaagt gataatcaat cgagtgataa
      421 agttgatatg cagcaactaa atcaagaaga caatactaaa aatgataatc aaaaagaaat
      481 ggtatcatct caaggtaatg aaacgacttc aaatgggaat aaatcaatag aaaaagaaag
      541 tgtacaatct accactggaa ataaagttga agtttcaact gccaaatcag atgagcaagc
      601 ttcaccaaaa tctacgaatg aagatttaaa cactaaacaa actataagta atcaagaagc
      661 gttacaacct gatttgcaag agaataaatc agtggtaaat gctcaaccaa ctaatgagga
      721 aaacaaaaag gtagatgcca aaactgaatc aactacatta aatgttaaaa gtgatgctat
      781 caagagtaat gctgaaactc ttgttgataa caatagtaat tcaaataatg aaaataatgc
      841 agatatcatt ttgccaaaaa gtacagcacc taaacgtttg aatacaagaa tgcgtatagc
      901 agcagtacag ccatcatcaa cagaggctaa aaatgttaat gatttaatta catcaaatac
      961 aacattaact gtcgttgatg cagataaaaa caataaaatc gtaccagcac aagattatct
     1021 agaattaaaa tcacaaatta aagttgatga caaagttaaa tcaggtgatt atttcacaat
     1081 aaaatactca gatacagtac aagtatatgg attgaatccg gaagatatta aaaatattgg
     1141 agatattaaa gatccaaata atggtgaaac aattgcgact gcaaaacatg atactgcaaa
     1201 taatttaatt acatatacat ttacagatta tgtcgataga tttaattctg tacaaatggg
     1261 aattaattat tcaatttata tggatgctga tacaattcct gttagtaaaa acgatgttga
     1321 gtttaatgtt acgataggta atactacaac aaaaacaact gctaacattc aatatccaga
     1381 ttatgttgta aatgagaaaa attcaattgg atcagcgttc actgaaacag tttcacatgt
     1441 tggaaataaa gaaaatccag ggtactataa acaaacgatt tatgtaaatc catcggaaaa
     1501 ttctttaaca aatgccaaac taaaagttca agcttaccac tcaagttatc ctaataatat
     1561 cgggcaaata aataaagagg taacagatat aaaaatatat caagttccta aaggttatac
     1621 attaaataaa ggatacgatg tgaatactaa agagcttaca gatgtaacaa atcaatactt
     1681 gcagaaaatt acatatggcg acaacaatag cgctgttatt gattttggaa atgcagattc
     1741 tgcttatgtt gtaatggtta atacaaaatt ccaatataca actagcgaaa gcccaacact
     1801 tgttcaaatg gttacattaa gttcaaacaa tagtaaatct gcctcaatgg gtaatgcttt
     1861 aggattcact aataaccaaa gtggcggagc tggtcaagaa gtatataaaa ttggtaacta
     1921 cgtatgggaa gatactaata aaaacggtgt tcaagaatta ggagaaaaag gcgttggcaa
     1981 tgtaactgta actgtatttg ataataatac aaatacaaaa gtaggagaag cagttactaa
     2041 agaagatggg tcatacttga ttccaaactt acctaatgga gattaccgtg tagaattttc
     2101 aaacttacca aaaggttatg aagtaacccc ttcaaaacaa ggtaataacg aagaattaga
     2161 ttcaaacggc ttatcttcag ttattacagt taatggcaaa gataacttat ctgcagactt
     2221 aggtatttac aaacctaaat acaacttagg tgactatgtc tgggaagata caaataaaaa
     2281 tggtatccaa gaccaagatg aaaaaggtat atctggcgta acggtaacat taaaagatga
     2341 aaacggtaac gtgttaaaaa cagttacaac agacgcagat ggcaaatata aatttactga
     2401 tttagataat ggtaattata aagttgaatt tactacacca gaaggctata caccgactac
     2461 agtaacatct ggtagcgaca ttgaaaaaga ctctaatggt ttaacaacaa caggtgttat
     2521 taatggtgct gataacatga cattagatag tggattctac aaaacaccaa aatataattt
     2581 aggtaattat gtatgggaag atacaaataa agatggtaag caggattcaa ctgaaaaagg
     2641 tatttcaggc gtaacagtta cattgaaaaa tgaaaacggt gaagttttac aaacaactaa
     2701 aacagataaa gatggtaaat atcaatttac tggattagaa aatggaactt ataaagttga
     2761 attcgaaaca ccatcaggtt acacaccaac acaagtaggt tcaggaactg atgaaggtat
     2821 agattcaaat ggtacatcaa caacaggtgt cattaaagat aaagataacg atactattga
     2881 ctctggtttc tacaaaccga cttacaactt aggtgactat gtatgggaag atacaaataa
     2941 aaacggtgtt caagataaag atgaaaaggg tatttcaggt gtaacagtta cgttaaaaga
     3001 tgaaaacgac aaagttttaa aaacagttac aacagatgaa aatggtaaat atcaattcac
     3061 tgatttaaac aatggaactt ataaagttga attcgagaca ccatcaggtt atacaccaac
     3121 ttcagtaact tctggaaatg atactgaaaa agattctaat ggtttaacaa caacaggtgt
     3181 cattaaagat gcagataaca tgacattaga cagtggtttc tataaaacac caaaatatag
     3241 tttaggtgat tatgtttggt acgacagtaa taaagacggc aaacaagatt caactgaaaa
     3301 aggtatcaaa gatgttaaag ttactttatt aaatgaaaaa ggcgaagtaa ttggaacaac
     3361 taaaacagat gaaaatggta aatatcgttt cgataattta gatagcggta aatacaaagt
     3421 tatttttgaa aagcctgctg gcttaacaca aacaggtaca aatacaactg aagatgataa
     3481 agatgcagat ggtggcgaag ttgacgtaac aattacggat catgatgatt tcacacttga
     3541 taa
//
LOCUS       DAMZLE010000193         3525 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMZLE010000193 DAMZLE010000000
VERSION     DAMZLE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.197"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..222
                     /locus_tag="POB92_002556"
     CDS             <1..222
                     /locus_tag="POB92_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6580011.1"
                     /translation="PDDDNRFVASVTQQPIQLLFNDNNQLYGFVYPIVDKKELKDKFN
                     INNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            231..1067
                     /locus_tag="POB92_002557"
     CDS             231..1067
                     /locus_tag="POB92_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580012.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(1328..2305)
                     /locus_tag="POB92_002558"
     CDS             complement(1328..2305)
                     /locus_tag="POB92_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6580013.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            2457..3494
                     /gene="mutY"
                     /locus_tag="POB92_002559"
     CDS             2457..3494
                     /gene="mutY"
                     /locus_tag="POB92_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI6580014.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRREKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         1215 a          511 c          603 g         1196 t
ORIGIN      
        1 cctgatgatg ataaccgttt cgttgcatcc gttacacaac aaccgataca attacttttc
       61 aatgataata atcagttata cggttttgtt tatccaattg tagataaaaa agaattaaaa
      121 gataagttta atattaacaa taacatttgg attactaaag ttgggaatgg atattgtatt
      181 gccaatttga aagaagacaa atggatttat attgaattgt aggtgtaaag atgctagata
      241 atattatttt atattttaaa aacttaccgc atactaaaag gtatgtaaca gaacgtttaa
      301 aacaaagttg gaaaagtttt ctaatcgtat tagctgcatg tttaatactt attattgcaa
      361 gtgaaacgct attttccttt agtcatttga ccgatgttaa agaggtgaga tggttattta
      421 gaatcattgt attgattgtt tttgcggtag tgatgttcac aatttatatc tcttatcatc
      481 attatatgaa tgacttttta gttactaagt tatttaatat ttccgcagcg acgccagtag
      541 ttattatgtc tattttaagt tttattatgc tagtcatttt gacgatgatt agtgcattgg
      601 ttaaaccagt aaacttcgaa acatcatata tagcgctatt ctattttatt gtgatggcaa
      661 ctatttttgt aggattaata tccgttactt ttggtcttat tcgactgtta actgaaaaaa
      721 taaatatcat attttatggg gtttgtgtat tgtgtttctt attgttacca attattttca
      781 taccaaatcc aaatcatgta tttatcaatc acatcctaat gctaaatcca atgtattaca
      841 ttgttaatgg tatagcacaa tctatcatat ttggtatatc aagtatggaa aacattccat
      901 atcattttta ctttatttta ttcttatgtt taatagctgc agtaaatttc gtattagcaa
      961 ggtatacgac acacgcaatt tataataaaa catctaaagt gacacaaact gataatcaac
     1021 aggatgtttc aaatgatagt acagacgagg cggatacttc ttcatgatgt ttagctgacc
     1081 tgttgtgtaa attaatctgt gagaatcaga aacaattaga atatcatcta gatattgttt
     1141 tcctatactg attttgatat ttcgagtcat taaaggtagt ttgttagcaa caatataaat
     1201 tttagatttt gtaaaagcat tcatagtttt acattaaaaa accggacaga gtaaaaatcg
     1261 ttttgatttt cattctgccc ggttgttcat tttttataga ttaactttag tgaataataa
     1321 cttgaaatta tttagcatac agtttacgtt gtaacttatc ttctgtaaat acccagccaa
     1381 tataagagtg gtctatgaca ttatcgtttg ttacatgtgc aattgccaca aatgaataat
     1441 gatcattttt taaataacgc aaatcaatca aacgaatttc agtagaacca tctgctaacg
     1501 ttgttgtttg ccaacgatag attgatgaaa agtttaaaaa agtacgtata tctttattac
     1561 ctttgacttt ccataaaatg gagtctgttg ataatgtttg acgttccact ttatcactaa
     1621 acaccacatt cctaccatat gcttttccaa catagtcatg tgcatcagtt tgtatcgcga
     1681 cacgccattc cataaatttt attgttggcg caacaaatac tttaacaggg tgatgctctt
     1741 gttgaattgc ttttaaagct tgttgcttaa ttacggctct cattttaaaa cgaatcatgt
     1801 aataaatgat tagtaaagcg attataggaa agaagactgc aaatggatgc aagcctataa
     1861 cccataatac aataccaata caaagaactg tgaaaataat agggtcaaat gtgttaatca
     1921 cacttaattg aatccatttg tttgtgatag gtctaagcgc ttgtgtacca taagaattga
     1981 atatatctac aaagacatgt aaaaatactg cgagctgagc ccacatccat acatgaaatg
     2041 ggttggttcc actaaagaac gtgaatatta aaaatgtaat taaaattggc catagtattg
     2101 tgaaagggat ggaatgcgtg atacctctat gatgcgaaat atatgttgca ttgtccttta
     2161 atttaagaac agtatcccca tcaggaatta atgaaccaac gataagggtt gtagctgttg
     2221 caccaaacgt agaagccatt gctggatctt gagttgcaag tgctgtaagg cccaccccaa
     2281 ttgcgatatg tgttgcagtg tccatatcag ttcactcatt tcataatatt atatatacta
     2341 ttataatcca atttcgcaaa taactaaatt aacttacata ctaaccatga ttttaatgta
     2401 caataaaaac attatgagta aagacaatta attttggtga ggaaagggtt ttataaatgt
     2461 atcagcagtc tagttttaaa gaaaatttga tacactggtt tgatgaaaat caaagagaaa
     2521 tgccatggcg acaaacgacc aatccgtact atatttggtt aagtgaggtc atgttacaac
     2581 agacacaagt taaaacagtt attgactatt atcatcgttt tgttgaacga tttccaactg
     2641 tggaagtttt gagtcaagct tcggaagatg aagtgttaaa gtattgggaa ggcttaggat
     2701 attatagtag agctcgaaat tttcatacgg cgattaaaga agtttatgat aaatatgaag
     2761 gcttagttcc caaagatcct gatcaattta aagcgttaaa aggtgttggc ccatacacac
     2821 aagctgcagt aatgagcatt gcatataatg taccactagc aactgtagac ggaaatgttt
     2881 ttcgagtatg gtctagattg aatgatgact atcgagatat taaattacaa tcgacgagga
     2941 aatcgtacga acaagaattg ttaccgtatg ttactacaga agctggaacg tttaatcaag
     3001 ctatgatgga attaggggca ttaatttgta caccaaaaaa tccattatgc ttattttgtc
     3061 cagttcaaga aaactgtgag gcatttgata aaggtacatt tgaaaagtta cctgttaaat
     3121 ctaaaaatgt aagtaaaaaa gtaattgagc aatccgtctt tttaattaga aataatcaag
     3181 gtcaatatct gttgcaaaag cgtagagaaa aattattgca tggtatgtgg caatttccta
     3241 tgtttgaaag tgagcatgct agacgtaaaa tgactgaaaa aatcggtcat gacattcaac
     3301 cagtagaaac acctattttt gagttgaagc atcaatttac tcatttaaca tggaagatta
     3361 aagtatacgc cgtttcagga gcaattaaca ttgaaacatt accagatgac atgatttggt
     3421 ttgatttaag tgatcgagat caatatacat tccctgtacc tatgtcaaag atttatcaat
     3481 ttataaatgg gtaacattcg atgattggta atattatcgg aaaac
//
LOCUS       DAMZLE010000194         3484 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMZLE010000194 DAMZLE010000000
VERSION     DAMZLE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.198"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            90..245
                     /locus_tag="POB92_002560"
     CDS             90..245
                     /locus_tag="POB92_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580015.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            253..774
                     /locus_tag="POB92_002561"
     CDS             253..774
                     /locus_tag="POB92_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580016.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREEDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDCFN
                     TYNNKEIYDLQFI"
     gene            913..1443
                     /locus_tag="POB92_002562"
     CDS             913..1443
                     /locus_tag="POB92_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6580017.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1706..>3484
                     /gene="clfA"
                     /locus_tag="POB92_002563"
     CDS             1706..>3484
                     /gene="clfA"
                     /locus_tag="POB92_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDI6580018.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSA
                     LTNATTEETPVTGEATTATNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVN
                     SPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSLS
                     AVAADAPAAGKDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNSAVKGDTFKI
                     TVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDTKENVTANITM
                     PAYIDPENVTKTGNVTLTTGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNT
                     YRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVN
                     PENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTL
                     YGYDSRFVWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGS
                     DSGSDSNSDSGSDSGSDSTSDSGSDSA"
BASE COUNT         1306 a          486 c          657 g         1035 t
ORIGIN      
        1 tggaaaatac tggtcaaaga tgggagctct caaaagcgtt attgtatttt ttagtcaata
       61 caaatagatt gccgtaataa taatcgtact tgatggttaa aaaattactt aaggctataa
      121 agcaaaactt tttatatgag cagtcgaata taacgtttaa aatgattgtt tttggatata
      181 aacgattaag taaaatgctt tttcagtttg aaattaatca tataaatttc ttatgggaag
      241 gttgatatct taatgattaa tattatttca gctataggat ctattggaac atttattatg
      301 gctttatttt attttgtatc agtttcagtt caactttatc aaatgaaaat tagctttctg
      361 ccagctttag gttttaacca aattttatta gaaagggagg aggatcaact taatataatg
      421 aattcggcaa cagaagagca tcatcataaa gattatatta aactatataa tttaggtggc
      481 ggtgctgcta aaaaaattgc aatagaggtt ttattgggta atgataaagt cattcagaaa
      541 aaatacgtga atattttacc tagtaaagaa gggtacatgt taccaattaa taaaaatgtg
      601 tacgaagaat tagaaagaac gattgagaac aatggttatg aagctgattt gaatgtacgt
      661 atgacttatt atcataatgt aagtcgcaaa caacaggaag ttatattaaa aggtcaaatc
      721 gactgtttta atacttataa taataaagaa atttatgatt tgcagtttat ctaaaaattg
      781 atttaagagg gtagttgttt attgcgaaaa atatcattca attttaatga aataatggcg
      841 tcattactat aaaatattac tttatgttgt aatgcatttt tctataagat agaactaaaa
      901 ggaggggcaa agatgcaaat tagacaaata catcaacatg actttgctca agttgaccag
      961 ttaattagaa cggcatttga aaatagtgaa catggttatg gtaatgaatc agaactagta
     1021 gaccaaattc gtctaagtga tacgtatgac aataacttag aattagtagc tgttcttcaa
     1081 aatgaagttg tagggcacgg tttactaagt gaagtttatc ttgataacga ggcacaacgg
     1141 gaaattggat tagtgttagc acctgtatct gttgatattc atcatcaaaa taaaggtatt
     1201 gggaagcgat tgattcaagc attagaacga gaagcaatat taaaaggata taattttatc
     1261 agtgtattag gatggccgac gtattatgcc aatctaggat atcaacgcgc aagtatgtac
     1321 gacatttatc caccatatga tggtatacca gacgaagcgt ttttaattaa agaattaaaa
     1381 gtgaacagtt tagcgggaaa aacaggtacc ataaattaca catctgcttt tgaaaaaata
     1441 tgatttcaag ctaggattac attaggtaga gttcatatta ataataaaaa atgtttgcaa
     1501 tgaaatcgtg cattgtcgtt tgcaattttt aaaatagcaa taaataaaat gtttgttagt
     1561 aaagtattat tgtggataat aaaatatcga tacaaattaa ttgctataat gcaattttag
     1621 tgtataattc cattaacaga gattaaatat atctttaaag ggtatatagt taatataaaa
     1681 tgacttttta aaaagaggga ataaaatgaa tatgaagaaa aaagaaaaac acgcaattcg
     1741 gaaaaaatcg attggcgtgg cttcagtgct tgtaggtacg ttaatcggtt ttggactact
     1801 cagcagtaaa gaagcagatg caagtgaaaa tagtgttacg caatctgata gcgcaagtaa
     1861 cgaaagcaaa agtaatgatt caagtagcgt tagtgctgca cctaaaacag acgacacaaa
     1921 cgtgagtgat actaaaacat cgtcaaacac taataatggc gaaacgagtg tggcgcaaaa
     1981 tccagcacaa caggaaacga cacaatcagc attaacaaat gcaactacgg aagaaactcc
     2041 ggtaactggt gaagctacta cggcaacgaa tcaagctaat acaccggcaa caactcaatc
     2101 aagcaataca aatgcggagg aattagtgaa tcaaacaagt aatgaaacga cttctaatga
     2161 tactaataca gtatcatctg taaattcacc tcaaaattct acaaatgcgg aaaatgtttc
     2221 aacaacgcaa gatacttcaa ctgaagcaac accttcaaac aatgaatcag ctccacagag
     2281 tacagatgca agtaataaag atgtagttaa tcaagcggtt aatacaagtg cgcctagaat
     2341 gagagcattt agtttatcgg cagtagctgc agatgcaccg gcagctggca aagatattac
     2401 gaatcagttg acgaatgtga cagttggtat tgactctgga gatacagttt atccgcacca
     2461 agcaggctat gtcaaactga attatggttt ttcagtgcct aattctgctg ttaaaggtga
     2521 cacattcaaa ataactgtac ctaaagaatt aaacttaaat ggtgtaactt caactgctaa
     2581 agtgcctcca attatggccg gagatcaagt attggcaaat ggtgtaatcg atagtgatgg
     2641 taatgttatt tatacattta cagactatgt tgatactaaa gaaaatgtaa cagctaatat
     2701 tactatgcca gcttatattg accctgaaaa tgttacaaag acaggtaatg taacattgac
     2761 aactggcata ggtagtacaa cagcaaacaa aacagtatta gtagattatg aaaaatatgg
     2821 taagttttat aacttatcta ttaaaggtac aattgaccaa atcgataaaa caaataatac
     2881 gtatcgtcag acaatttatg tcaatccaag tggagataat gttattgcgc cggttttaac
     2941 aggtaattta aaaccaaata cggatagtaa tgcattaata gatcagcaaa atacaagtat
     3001 taaagtatat aaagtagata atgcagctga tttatctgaa agttactttg tgaatccaga
     3061 aaactttgag gatgtcacta atagtgtgaa tattacattc ccaaatccaa atcaatataa
     3121 agtagagttt aatacgcctg atgatcaaat tacaacaccg tatattgtag ttgttaatgg
     3181 tcatattgat ccgaatagca aaggtgattt agctttacgt tcaactttat atggatatga
     3241 ctcaaggttt gtatggagat ctatgtcatg ggacaacgaa gtagcattta ataacggatc
     3301 aggttctggt gacggtatcg ataaaccagt tgttcctgaa caacctgatg agcctggtga
     3361 aattgaacca attccagagg attcagattc tgacccaggt tcagattctg gcagcgattc
     3421 taattcagat agcggttcag attcgggtag tgattctaca tcagatagtg gttcagattc
     3481 agcg
//
LOCUS       DAMZLE010000195         3476 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMZLE010000195 DAMZLE010000000
VERSION     DAMZLE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.199"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            3..392
                     /locus_tag="POB92_002564"
     CDS             3..392
                     /locus_tag="POB92_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDI6580019.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHKMFWI
                     TMALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(564..1880)
                     /locus_tag="POB92_002565"
     CDS             complement(564..1880)
                     /locus_tag="POB92_002565"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDI6580020.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1932..2756)
                     /locus_tag="POB92_002566"
     CDS             complement(1932..2756)
                     /locus_tag="POB92_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6580021.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2870..3178
                     /locus_tag="POB92_002567"
     CDS             2870..3178
                     /locus_tag="POB92_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDI6580022.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT         1085 a          561 c          588 g         1242 t
ORIGIN      
        1 gtatgttaca tttacatata ttaagttggg tattagcgat tattttattt atcgctacat
       61 acttaaacat ttcaaaaaat caaggcggat caccattttt caaaccgttg cacatgattt
      121 tacgcttatt tatgctgttg acgttaattt caggattttg gatattaatt cagtcattta
      181 tgaatggcgg ggcaaatcat atgttgctta cattgaaaat gctgtgtggt gttgcagtag
      241 ttggattgat ggaagtgtcg attgctaaaa gaaagagaca tgaacaaagt cacaaaatgt
      301 tttggataac aatggcatta attatcatca caatggtatt aggtgtcatt ctaccgttag
      361 ggcctatatc aaaattattc ggtattggct aattgataag acatatagta tatgacataa
      421 ggcatggaca attattagtc caagacctta tgtctttttt gattctaaaa atgtttaaac
      481 ataaaaaaag gactggatag tagtaccagt ccaacataat tctaacctag acaattaaga
      541 ttagattgaa tcgcatgatt catttattta gctttgtaac caatcatatt gattaaatct
      601 ttagggtggc tatatggtgg tgcataagcc acttcaaact cagttaactc atctacagtt
      661 agctggttca tcattgccat cgatagtaca tcaatacgtt tatctgcacc ttcttttcct
      721 actgcagctg ctcttaaaat ctgacggttt gaagtgtcat aatataccct taagtgtaaa
      781 ggggaatttc ctgggtaata attcgcgtgt gcaccttgag tgacttccac cattttatag
      841 tcaaattgct ttagttcatt tggtttaacg ccgacactcg caaatgtata atcaaagaac
      901 ttcacaatat tgttgcctaa gaagcctttg aattcaatag tgtcatttcc agcaatttgt
      961 tcggcaacaa tacttgctgc acggtgagcg ccccaagcta aaggaacact agccggtaga
     1021 tcgacatgtc gataatgtga tgttgcaata tcgcctattg cataaatgtt tggaacattt
     1081 gtttcaaatt tatcgtttac cggtatgaaa ccttttcgat caagtttgat atttgaactt
     1141 tcgataaatt ttgaattggg gtgagtaccg acaccttcaa taatcatatc gtaatgttca
     1201 acttttcctg atttaaatgt aatttcattt ccattgatag catcaatttc ctcatttaaa
     1261 cggtatggaa tctcccgctt atctaattca tcaagtatag gttgattcat gtcagcatcc
     1321 attaatttat ttatcttatc agatcgatga attaaagtag ggtgtaaacc acgttcgtaa
     1381 agattttcaa gaacttctaa tgaaacatac cctgcaccta caactaatac tttatcaact
     1441 tgatttgctt tgatgaattg atcgatagca tcagtgtctt ctaaatttct aagtgtaaat
     1501 gtaatatcac tttcaaagcc aaggctattt gcacttgcac cagggcttaa aatgagttta
     1561 tcgtaagatt cttcaaattg ttcgttggtc tttctattta atacagttac agtttgtctt
     1621 tcatcattga ttgcaataac ttcatgataa gtttttactg taatttgctt tctatcataa
     1681 aatttttcag gtgtatacgc taaagcatat tttctatctt caacaacttc gccaatgaca
     1741 taaggcaatg cacaattagc aaagctcata tcacgatctt tttcaaaaat aataatgtca
     1801 ctttctttat ctaaacgtcg aatttggctg gcacatgttg caccgccagc gactgctccg
     1861 actacgacta ttttgggcat cgtaacttac ctcctattgt tttaaaatat tataaaatga
     1921 ttagttataa attaacagta atatctaata tttaaattaa agaaatcatt caaatatcga
     1981 ccaatgccat cttcattatt gttgaatgta atattgttcg ctacatcttt aagttcttgc
     2041 aaaccatttt ccatagcaac accatggcgg gcgtactcaa tcatttcaat atcattatct
     2101 tcatcaccga atgcaataat attatttcgg tcaatattta aaaattgtct aacttgctca
     2161 atgcctcttg ctttattaat accaagtttt acaatttcaa tgacagggaa tggtgcgccc
     2221 cagcgtcgat gctcaatatg atcggcataa aaatgagtaa gcatattttt gatttcaggt
     2281 attttacttt cttcggcttc aattaaaatt gaggtagggg attctttcaa gtggacaagt
     2341 aaattaccag tttgaattct tggattaccc attgaaaaac cttcaaataa tcttggatca
     2401 tgattgttaa tgaaaacata atctttcact tctgctataa tattcgatac ttgatattgt
     2461 tgtaatcctt gaataatgtt ttgtgcgatg cctaaatcta aaatttcatg gcaagttttg
     2521 aagtttttat ctttagggtg atgtacgtaa gcgccattaa aattaacaat tggtgtcgtt
     2581 aaatttaatt catgataata catttgactt gcacgataag gtctgccagt cgcaatcata
     2641 atttggtgtc cacgttgttg taattcattt aatacttgtt tagtatatga tgaaatttct
     2701 ttgttatcgt ttaataatgt tccgtctaag tctagacata ttaaatgtgg ttgcatatct
     2761 ctctcctcat aagttgctta gttcatatag atacatcata gcattttatt ggcgttttgt
     2821 cggatatttg atatattatg aataatatga cattaaagag gtgatatgga tggaagaggc
     2881 attgaaagat agtatcttag gtgcattaga aatggtaatt gaccctgaat taggaattga
     2941 tatcgttaat ttaggtttag tatacaaagt gaatgttgat gatgaaggcg tatgtacagt
     3001 tgatatgact ttaacatcaa tgggatgtcc aatgggacct caaattattg atcaagttaa
     3061 aacagtatta gcagagattc ctgaaataca ggatactgaa gtgaatatcg tatggagtcc
     3121 accttggaca aaagatatga tgtcacgtta cgctaagatt gcacttggtg tgagctaaca
     3181 taaacgtgca ttgcctgtag aatttctttt agaaattctc tgtgttgggt ccccgcagtc
     3241 aatataacat tgtagagtat aattcattgt gaataagctc ccttcaaagt agacattgaa
     3301 aaatgaaaat tttgaaggga gccttattta tgttctaggt tctgatttca ttttgaaaat
     3361 ttatatctaa cttgtttcaa aaaatatgaa ttaagacagt gacttgcact tctaagagtg
     3421 cgcaattcat tttctagttg aatattttct tgaaaatttt tataataaat tgaatt
//
LOCUS       DAMZLE010000196         3463 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMZLE010000196 DAMZLE010000000
VERSION     DAMZLE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.200"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..503
                     /locus_tag="POB92_002568"
     CDS             <1..503
                     /locus_tag="POB92_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDI6580023.1"
                     /translation="LQWIRFIDFEKESTQKYKKIYARFLVIINVLTTITVVFALCNLY
                     YFAAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHFGLL
                     LTALSSFIYIEKIIEYILIPNVVESKFIIIVSMMVIAGAQFVAFQFIMQYSRFYIFEL
                     NTEDDD"
     gene            650..1261
                     /locus_tag="POB92_002569"
     CDS             650..1261
                     /locus_tag="POB92_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDI6580024.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVVIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAFRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLIIRVRKGAFAFVVIRPILFSCWLFILNMILSRLV"
     gene            1460..1960
                     /locus_tag="POB92_002570"
     CDS             1460..1960
                     /locus_tag="POB92_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580025.1"
                     /translation="MTFEEKLNEMYNEIANKISSMIPVEWAKVYAMAYIDEESGEVFF
                     NYTEPRSDELFYYTSVIKKYNISRSGFMNSVYELHDQFEKLRELFIEEGHEPWTSCEF
                     DFTKEGKLKVSFDYIDWLNTEFDQLGRENYYMYKKFGVIPEMEYEMEEIKEIEQYIKE
                     QDEAEQ"
     gene            1971..2471
                     /locus_tag="POB92_002571"
     CDS             1971..2471
                     /locus_tag="POB92_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580026.1"
                     /translation="MTFEEKQSEMYNKIANEISGMIPVEWEKVYTIAYVDNQGGEVIF
                     NYTKPGSEELNYYSDIPKDCNVSNDIFMDLWMKVYRMFDELRETFKKEGHEPWTSCEF
                     DFTRDGKLKVSFDYIDWLNTEFDQLSLENYYMYKKFEVIPEMEYKMEEIKEIEQYIKE
                     QEEAEI"
     gene            2482..2982
                     /locus_tag="POB92_002572"
     CDS             2482..2982
                     /locus_tag="POB92_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580027.1"
                     /translation="MTFEEKLNEMYNEISNKISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPNSDELNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRNLFKEEGLEPWTSCEF
                     DFTREGTLNVSFDYIDWVNTEFDQLGRENYYMYKKFGVIPEMEYEMKEIKEIEQYIKE
                     QDEAEI"
     gene            2993..>3463
                     /locus_tag="POB92_002573"
     CDS             2993..>3463
                     /locus_tag="POB92_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580028.1"
                     /translation="MAFEEKLNEMYNEIANKISSMIPVEWETVYAMAYVNERSGEVFY
                     NYTEPSSDELFYYTSVLNKYNIPRSEFMDSVYELYKQFDNLRELFIEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVKKIKDY"
BASE COUNT         1320 a          330 c          661 g         1152 t
ORIGIN      
        1 tacttcaatg gattagattt atagacttcg aaaaagaaag tacacagaaa tataaaaaaa
       61 tatatgctcg atttttagtt attataaatg tgttaactac tatcactgta gtatttgcac
      121 tgtgtaacct ttattatttt gcagctgtac aaaaccatta cgatttattc aattattggt
      181 taatgggtac gatttcaatc ataattagct atttattatt agtaattggc ggaatgttta
      241 cgttgttaaa attacctaaa gtaacaaaac gctggggggg taaaactaaa acacatttcg
      301 gtttattatt aactgcgttg agctcattta tatatattga aaaaattatc gaatatatat
      361 tgattcctaa tgtcgtagaa tccaagttta taattattgt gagcatgatg gttatcgccg
      421 gagcacaatt tgtggcattt caatttatta tgcaatacag tagattctat atttttgaat
      481 taaacactga agatgatgac taaatgaatt tataaattac tgttaaagta tacttaaata
      541 agaaaatcaa cttcaacgta tatcgccata ttattttata ctcaatgatg tgaatggatt
      601 taagtggatt gatattaaag taaaagttat ggaatattga ggtgcgaata tggagttctt
      661 attattaatt gtcgtagccg gactgtatta tattatatat ttaactgctg tgatgtattc
      721 tgaaaaaata gtagtattgc ctataatcat ctatgccatt gtgtttgtag taattggtat
      781 cacttatatt tttataggtg acagctatga tcagttaaca aatttcaatg tgattttgta
      841 tatggggagt ttgttttatg catggatggc ttttagaaat ctttggaaca gaccattatt
      901 attaaaatat aagaacatta cagatagttc aagtggaata gttaataaat ctgaatataa
      961 ttcagttgaa agcttacgta taaacattga aatagctaag tataaaggga ttatttcttt
     1021 gatagtagct atagtactaa cggtattaat gacattaaag tcaacgcctc aaattactgc
     1081 ggaaacacgt gacttaagta tctcattttt catactcagc ttatttatca ttattatatt
     1141 tgctgtttgg gatttaatta ttagagttag aaaaggagcg tttgcttttg ttgtaataag
     1201 gccaatatta ttcagttgtt ggttatttat tctgaatatg attttatcaa gattagtata
     1261 atgataagaa gttgaataaa ttgtaatgtg aaaaatcatt gagaatttct attattgtaa
     1321 caatagtttc agttatttta gtttcattag tttatttagg tttaaaatga gtaatattat
     1381 tttgaagaag cattaagcca cgtagaggtg ttgtttaatg ctttttattt aacggtaatg
     1441 aaaatatagg ggagataaca tgactttcga agaaaaacta aatgaaatgt acaacgagat
     1501 tgcgaataag attagcagca tgataccggt agagtgggca aaggtatatg caatggcata
     1561 tatcgatgaa gaatctggag aagtgttttt caattatact gaaccaagaa gtgatgaatt
     1621 attttactat acaagcgtga taaaaaagta taatatttcg agatcaggat ttatgaattc
     1681 agtatatgag ttgcatgatc aatttgaaaa actaagagaa ttgtttatag aagaaggaca
     1741 tgaaccatgg acatcatgtg agtttgattt tacaaaagaa ggtaaattga aagtatcttt
     1801 tgattatata gattggttaa atacagaatt tgatcaattg ggtcgtgaaa attattatat
     1861 gtacaaaaaa tttggggtta taccagaaat ggaatatgaa atggaagaaa ttaaagaaat
     1921 cgagcaatat attaaagagc aagatgaagc tgaacaatag aggcgataac atgactttcg
     1981 aagaaaaaca aagtgaaatg tacaataaaa ttgcaaatga gattagtgga atgataccgg
     2041 ttgagtggga aaaggtatat acaattgctt atgtagataa tcaaggtgga gaggtcattt
     2101 ttaattatac taaacctgga agtgaagaat tgaattatta ttcagatatt cctaaagatt
     2161 gcaatgtctc aaatgatatt tttatggatt tatggatgaa agtttatcga atgtttgatg
     2221 agttaagaga aacttttaaa aaagaaggac atgaaccatg gacatcatgt gaatttgatt
     2281 ttacaagaga tggtaaattg aaagtatctt ttgattatat agattggtta aatacagaat
     2341 ttgatcaatt gagtcttgaa aattattata tgtacaaaaa attcgaggtt ataccagaaa
     2401 tggaatataa aatggaagaa attaaagaaa tcgaacaata tattaaagag caagaagaag
     2461 ctgaaatata ggggagatga catgactttc gaagaaaaac taaatgaaat gtacaacgag
     2521 atttcgaata aaattagtag catgatacca gtagaatggg aaaaggtata tacaatggct
     2581 tatatagatg atggaggagg tgaagtattc tttaattata ctaaaccaaa cagcgatgaa
     2641 ttgaattatt acaccgatat acctaaggag tataacattt ctgtgcaagt atttgatgat
     2701 ttatggatgg atttatatga tttgtttgag gaattaagaa atttatttaa agaagaagga
     2761 ctagaaccat ggacatcatg cgaatttgat tttacaagag aaggtacatt gaatgtatct
     2821 tttgattata ttgattgggt taatacggag tttgatcaat taggccgtga aaattattat
     2881 atgtacaaaa aatttggggt tataccagaa atggaatatg aaatgaaaga aattaaagaa
     2941 atcgaacaat atattaaaga gcaagatgaa gctgaaatat aggggagata acatggcttt
     3001 cgaagaaaaa ctaaatgaaa tgtacaatga gattgcgaat aaaattagca gtatgatacc
     3061 ggtagaatgg gaaacggtat atgcaatggc ttatgtaaat gaaagaagtg gagaagtgtt
     3121 ttacaattat actgaaccaa gcagtgatga attattttac tatacgagcg tgttaaataa
     3181 atataatata ccaagatcag aatttatgga ctcagtatat gaattatata agcaatttga
     3241 taatttaaga gaattgttta tagaagaagg actcgaacca tggacatcat gtgaatttga
     3301 ctttacaaga gatggcaaat tgaatgtatc ttttgattat attgattggg tgaattcaga
     3361 atttggacca atgggaagag aacattatta tatgtataaa aaatttggaa tttggcctga
     3421 aaaagaatat gccataaatt gggttaaaaa aataaaagat tat
//
LOCUS       DAMZLE010000197         3443 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMZLE010000197 DAMZLE010000000
VERSION     DAMZLE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.201"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            153..1196
                     /locus_tag="POB92_002574"
     CDS             153..1196
                     /locus_tag="POB92_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI6580029.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            1199..1879
                     /gene="sdaAB"
                     /locus_tag="POB92_002575"
     CDS             1199..1879
                     /gene="sdaAB"
                     /locus_tag="POB92_002575"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDI6580030.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            1893..2792
                     /gene="sdaAA"
                     /locus_tag="POB92_002576"
     CDS             1893..2792
                     /gene="sdaAA"
                     /locus_tag="POB92_002576"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDI6580031.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            2811..2909
                     /locus_tag="POB92_002577"
     CDS             2811..2909
                     /locus_tag="POB92_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580032.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            2971..3141
                     /locus_tag="POB92_002578"
     CDS             2971..3141
                     /locus_tag="POB92_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580033.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
BASE COUNT         1152 a          529 c          729 g         1033 t
ORIGIN      
        1 gaaacaatcc attttaaaaa tctttcacaa atatgaaata tttcacttaa atatttgatt
       61 tgataacaga taaaagttga aaacgttttc ttaagggtgt acgatgttta tggtctgaaa
      121 ataaaataaa catagagaga ggtttttggg aaatggatat tctgttaggt gtaggcacat
      181 tagtcttggt tttagtgatc atgacgctat ttttaaagta tgcaccgtac ggtaaacaag
      241 gactacaagc attatccggt gcagcttgtg cgacatttct accacaagca ttcttaagtt
      301 acgctattgg tggcgtattt gatattaaat ttctacaaga tattggggat ttagcaggaa
      361 gtttaagcgg tatcgcagta ggtattctaa cgtgcttaaa cttaggcgtt gcaccagtat
      421 ttgcggttat tgttggttta gtattacatg actttaaatt attaccagca ttcatcgcag
      481 cttatggcgt agctttttta attaaatgga ttgaaaagaa agttccagaa ggcttggact
      541 taattgtggt gatcttattt gcaccagcaa ttgctttcgg acttgcatca atcattacac
      601 ctggtgtgct agcaacatta aaacaaatag gtagtgctgt cactgcggta ggtgataaca
      661 atccatatgc attagcggtc attttaggac tagtgattcc tgttacaggt atgacaccgc
      721 taagttcaat ggtattaaca agtttattag gactgacagg tgttcctatg gctattggag
      781 cactaacatg tacaggtagc tcatttgtta accttatttt attccgaaaa ttaaacattg
      841 gtggaccgtc aaaagcattt gcagtttgta ttgagccatt gacgcaaatt gatttaatag
      901 cgcaatatcc agtacaacta tatggtacaa atgcactgat tggggtcgtc aacgcatgta
      961 tcgtgacatt tagcggacta gtaataggtg tcaaaggtat ggcaacacca attgcaggcg
     1021 ccatcgtatt gtttggcttt aataatgcag tgacatcaat tgtaacaatc gcaacagtga
     1081 ttattgtcag cattgtctta gcctatatta ttggaacact aattaataag tttaatttaa
     1141 tgaatatcaa ctttaaaatg ccgagcaaaa agaatcatat taaggagagt gtttaattat
     1201 ggcaaagagc tatgattatc aaagtgcttt cgatattatc ggaccagtta tgatgggccc
     1261 ttcaagttct catacggctg gtgcagtaaa gattgggaat tcagcacgcg caatattggg
     1321 agatattcct aaaaatattg aaatacatta ttatgaatca tttgctcaaa cacaccaagg
     1381 acatggtaca gatgtcgcta ttgtgggtgg agcaatggga tattcaacat ttgataaccg
     1441 aattaaatct tcattagaaa ttgcagctga tgatggtatt aaaatagata tcatcgaaga
     1501 agaaggcgat agtattggtc aacatccaaa ctgtgcttat atcaaatcat ctcgtaatga
     1561 tggtcgctac attgaaatta tcggtatttc aatcggtggt ggtacaatta aaattaaagg
     1621 tattcatata aatggactag aagtagattt aaatcatgga ttaccaattt tagttgttga
     1681 cggaaatatg actaaagcac aagtaaatca tttcatcaat gatatcaacg atatgaaatt
     1741 agactgtaaa gatgaattta ttaagataga tgaagataaa tgcttggttg taataccatt
     1801 aaataaagca atctcagaat caacattaaa tacaattaaa gagaaataca gtgacgtaaa
     1861 cgtttcctat ataaattaga gggggaataa acatgtttga ttcaattaga gagactatag
     1921 attatgccgt agaaaataat atgtcatttg cggatatcat ggttaaagaa gaaatggaat
     1981 taagcggtaa atcacgtgat gaagtgcgag cgcaaatgaa acaaaattta gatgtcatgc
     2041 gagacgcagt aatcaaaggg acgacaggtg atggggttga aagtgtaacg ggctacactg
     2101 gtcatgatgc tgctaaacta cgtgattata atgaaacaca tcatgctttg tctggatatg
     2161 aaatgattga cgcagtcaaa ggtgccattg caacaaatga agtcaatgct gcgatgggta
     2221 ttatttgtgc aacgccaaca gctggttcct cgggtaccat tcccggtgca ctttttaaat
     2281 tagaaaaaac acatgattta acagaagagc aaatgattga tttcttattc acttcagcat
     2341 tgtttgggcg tgtcgtagca aacaatgcaa gtgtagctgg tgcaacaggt ggctgccaag
     2401 ctgaagttgg ttcagcatct gcaatggccg cagctgcagc agtagctata ttcggaggat
     2461 caccagaagc atccgggcac gctatggcat tagcgataag taatttatta ggtttagttt
     2521 gtgatccagt agccggactt gttgaaattc cttgtgttat gagaaatgca attggttcgg
     2581 gtaacgcttt aatttcagca gatttagcat tagcaggtat tgaaagtaga atccctgttg
     2641 atgaagttat tgaagcaatg gataaggttg gtcgtaacct tcctgcatca ttacgtgaaa
     2701 ctggattggg tggactagca ggcacaccaa ctggcgaagc aattaaacgt aaaatctttg
     2761 gcacagctga agatatggtt aaaaataatt aatgagataa caataaaata ttgataaaaa
     2821 agaagcatct gtctggatca ttagtaccag atagatgctt cttaattatc tctgaagaag
     2881 ttgaaagatt tatggaaaat gcatactaat caaaatagtg attgcaaaag cgtccccgta
     2941 tatctaaaat aacttaaaag gagtgatgat atgctaagac tatcaatcat ctttatagca
     3001 ttcatcatta acacaacgat tacatatgga tatacgactg agggaacgtg ggtcaatcta
     3061 ttatttaaat ctttatcgct cagcatgatt atcgttttca tgttctacta tattcgtttt
     3121 gttattgaga aaaagcgtta aatcaaagct atggggatag tatttgaaaa gaataaataa
     3181 aagggacgat gtaaatcaat gtaatatgct ctacaaagtt atattggcag tagttgactg
     3241 aacgaaaatg cgcttgtaac aagctttttt cgattctagt caggggcccc aacacagaga
     3301 atttcgaaat gaaattctac aggcaatgcg agttggggtg tgggccccaa cacagaagct
     3361 gacgaaaaat cagcttacaa taatgtgcaa gttggcgggg ccccaacaaa gagaaattgg
     3421 attcccaatt tctacagaca atg
//
LOCUS       DAMZLE010000198         3426 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMZLE010000198 DAMZLE010000000
VERSION     DAMZLE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.202"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1588
                     /locus_tag="POB92_002579"
     CDS             <1..1588
                     /locus_tag="POB92_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6580034.1"
                     /translation="NFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMNIEVPLSA
                     LLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTLIISQILLTTS
                     VLWILNYTGNLDLFILRLVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQHFKTI
                     RFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQ
                     SLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYHKQ
                     FQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKESKLSIQFINKN
                     DYRIFTFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIE
                     KYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQH
                     RKLLVIKKLFGYSTLRANYQYLLINNIVVVFIGILTNVILHSQYIMMIFATIIVVQIL
                     LQICSLYYHGRRFNEVIKEF"
     gene            1591..1911
                     /locus_tag="POB92_002580"
     CDS             1591..1911
                     /locus_tag="POB92_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6580035.1"
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1908..2549
                     /locus_tag="POB92_002581"
     CDS             1908..2549
                     /locus_tag="POB92_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6580036.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(2637..2927)
                     /locus_tag="POB92_002582"
     CDS             complement(2637..2927)
                     /locus_tag="POB92_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580037.1"
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
     gene            3268..>3426
                     /locus_tag="POB92_002583"
     CDS             3268..>3426
                     /locus_tag="POB92_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580038.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIDPTIEI
                     ENGNTKLKL"
BASE COUNT         1350 a          402 c          530 g         1144 t
ORIGIN      
        1 aaattttgac gtgtctaaat tcataaattt tttaaaagaa tatggtgtta ctgctgaatc
       61 atacaaaata gatcatatga tgattgccgt tggtgtcatt aaacaaatga atatagaagt
      121 tccgttatcc gcacttttaa tcgtttattt tatttattat attttcgaaa agaacattaa
      181 tttcaaagcg tatgcgatta agtatttaaa tggttttaca ttaagaaaaa taatttttga
      241 aaatttttca aaaaaatgca cgtattgggt aacgttaatc ataagtcaaa ttcttttaac
      301 tacaagtgta ctttggatat taaattacac gggtaattta gatttattca tattaagatt
      361 agttctgctt tcatgtcttt tcattttaac gattagtgtt attaatttat ggactttctt
      421 aatgttacta aatttaaata ttgctaatat gattaaaggt aagcagcact ttaaaacaat
      481 tcgttttatt aatacagttt gtaaaagtat tctcttagta ctaatagcta atgtaatgat
      541 agaaaatact agtgttatta aagatttgaa taaaataaaa gaaactgaga aatattggaa
      601 tgtattagat gattattaca cgattgaatt tgcaccttat cacgaaacaa aacaaagttt
      661 gattgataat atggtgcgat cagaacaatt agtaaaggct agtgaagcag aaaataatgc
      721 gattttattc aaaccaaagg gtgactccgt tgacaatgac aacttttcac ctgatgaggg
      781 gaatgtaata ttagtaaata atcagttttg gtcgatttat cacaagcagt ttcaacctga
      841 tattccaata aaaaatcaaa aaaataatgt cgaagtaatt attccacaaa agtttcatgc
      901 aatgcgtaat gaaatcaatc aagcatatca ttcatggttt gaatttgtac aaaataaaaa
      961 taataaagag agtaagttat ctatacagtt tatcaacaaa aatgattatc gaatttttac
     1021 atttgatgca cgagatagtc gccatttgtc atttatagag gcgccaatca ttgtgaatgt
     1081 tcaggcatca gatttatcga atgattttta ttatgccatg atcagtcaag gcgggtattt
     1141 attcaaaaat tatgacgcgc tagtaaaaaa tattgaaaag tatcatcttg atggggaaat
     1201 cagtggaata accaattata aagatagcgt gatggaaatg tatcatgaaa acaatttgaa
     1261 attaacagta ctcaactttt cacaaatcat tatcgcaatc attttaataa ttattatttt
     1321 atttgatgtg aaatattatt ttgaacaaca tcgaaaatta ctcgtaatca aaaagctatt
     1381 tggttattca acattaagag ccaattacca atacttatta ataaataata tagttgttgt
     1441 ttttattgga atattgacga atgtaatttt acattctcag tatataatga tgatatttgc
     1501 aacgattatt gttgttcaaa tattattgca gatttgcagc ctatactatc atggccgacg
     1561 ttttaatgaa gttatcaagg agttttaact atgaaatata tcattgcaat attattagga
     1621 ttaatcgtat ccattactat tgcttttaca atcatacatc atcctatact tgatcgtttt
     1681 aatcctttct taaaaacgga gtatagttat gccaaagtgc caaaaggtac gcaacaatat
     1741 gttaatatta cggcttatag tgaaagaggg gaaaagcttg attataaatt aacatttaat
     1801 ggattttcac ctagtagaac gtatgtcgaa ataaagcata aagggcaata tgtcatatcg
     1861 atcacatatg ttgaaaaaga ggatacacca aaagaggtaa gacaagaatg atagaattaa
     1921 aagatttaac catacaaaaa ggtaatacac atattttgaa gaaacttaat ttgaaatttc
     1981 aatgtggaaa atcatatgca cttattggta aaagtggatg cggtaaatct acattattaa
     2041 atactattgc tggacttgaa aaaacgggga aacaatatgt ctattttaat ggtcaattag
     2101 aacaatttaa atctaatttt tacagagata aattaggata tttatttcaa aattatggat
     2161 taatcgataa tttgacagta aatgaaaatt tagatattgg attagcatat aaaaaaataa
     2221 gtaagaaaga aaaagaacaa ttaaagatac gttatataga acagtttggt ctgtcaaaca
     2281 gtttaaaaag aaaagttcat acgctaagtg gaggtgaaca acaacgtgtc gctttaatta
     2341 gaatgatgtt aaaagatccg attgttatgt tagctgatga accaacgggt gcgttagatc
     2401 ctaaaacagg acagatgatt attcaatcat tatttgattt ggtcgatgaa aataaagtgc
     2461 tgattttagc aacacatgat atggctattg caaatcaatg tgacgaaata atagatttag
     2521 aacagtatag aaaagtagca tctatgtgat atccaaaaga taaaattaaa agccaatcaa
     2581 tgggaaatgt tgacttcaac actccgtttg attggctttt ataatgtgtt actaagttat
     2641 gaaagtaaca acggcaaact gaaaaaaagc aatataataa tatttaatac gattagtaat
     2701 aaatgtaatt gatttttcgt tttaatatag caaaaaacaa tgactaaaga taacaacaat
     2761 atcatgattg cacctgggaa gaaccataat gctgaagctg tgtaccaaat cattggtaac
     2821 gccataataa taaaagatat aagacaagaa aggataccaa taatattata agttctagtc
     2881 aaaattacac ctccaatttt cattttaaaa tgacatgcgt aaaacaatca ttttaaatac
     2941 atcatacaat aaaattaaat tgtgcggtag aagaatttga aattaaattg aattggctta
     3001 tctttcgtaa acataattat gacgtaacaa tccttataag tggaacgagt gtagttatac
     3061 catctgtata gagagtcgta tcaatcataa aatttaatct atgttgttat tgattttcat
     3121 actacaaata ccgttttaac attgtgtaaa atattatttt acttaaataa acgaaataaa
     3181 atattttaat tattgtatat atagttgctc taaatggttg cggagtataa aatatatttt
     3241 gttaaatatt ttattaagag gtgcgctatg gaaaagggga ttttcaatta cgataatgcg
     3301 aatgtattaa aattagatac taatcagtta aatgaaaaca taaaagtcat tgacgatata
     3361 tttaaaaatt atgagcagat agatccaact atagaaatcg aaaatggaaa tacaaaatta
     3421 aaatta
//
LOCUS       DAMZLE010000199         3349 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMZLE010000199 DAMZLE010000000
VERSION     DAMZLE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.203"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1480
                     /locus_tag="POB92_002584"
     CDS             <1..1480
                     /locus_tag="POB92_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6580039.1"
                     /translation="VGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLYRN
                     AQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQE
                     RFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFY
                     NPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFS
                     DRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIV
                     STEQQKLDIEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQ
                     QIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDA
                     YLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDEDALFD
                     KVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1604..3076)
                     /locus_tag="POB92_002585"
     CDS             complement(1604..3076)
                     /locus_tag="POB92_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6580040.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHHLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLADTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QVEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(3195..>3349)
                     /locus_tag="POB92_002586"
     CDS             complement(3195..>3349)
                     /locus_tag="POB92_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6580041.1"
                     /translation="STTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGR
                     RKKQNK"
BASE COUNT         1087 a          520 c          505 g         1237 t
ORIGIN      
        1 tgtaggtaat agtttaggtg ttaatttaac tgggatagag aaagcaatta ttaatcgttt
       61 aaatttattt aaagaaatgg gaagaccagc gcaatgtgta tttctgtcat ggaatagata
      121 tttatataga aatgcccaaa attatattac tagttctgac tatattaata tgtatgattt
      181 cttccaagaa gcaacatatt tagaacgcaa tgaaccattt gactggttgt cgtattggac
      241 tgatgaatgt cattatactt taaaacatgt tgaaaatagt catgacttta gaatatatga
      301 tcaagagcgt ttcttaatgt atgcccattt tcaagatcct aaatatcgta tattagatta
      361 tgtgaatcat tttgatagtc aaagacgaaa agtaaaacgt gatttttatg atgtgcgtgg
      421 ctttttaagt tgtagtagga ttcttgttga taaacaacaa acattatgtg aatttttcta
      481 taatccagaa ggtgacacaa aattagaaaa gtatttttct tacaaggatg gcaaaccaga
      541 agtacaaaaa attattgttt actatgctaa caaacaatat ttctttaata atgaaacgga
      601 attaggcgca tttttcatta aacaactata tcaacatggg gatttatttt tcagtgatcg
      661 taatgtgtac acagcaccta ttttcaactt aacgcctgag tcaatcccag tagtagctgt
      721 gcttcacagt acacacatta aaaatattga tgcattagat tcatcgccat ttaaaaatgt
      781 atataaagcg atgtttgaaa atttatctcg atatcgtgca attatcgttt ctactgagca
      841 gcaaaaatta gatattgaaa aacgtattaa ccatactatt ccagttgtta atattcctgt
      901 cggctatagt gaaacaattg atacaccagt ccaaacatta gatcagcgca gtgttaaatt
      961 aatttccgtt gctcgttatt cacctgaaaa acaattacat caacaaattg aattaattaa
     1021 acgcttagta tcgtacgtcc caaaaataga gcttcatatg tatggttttg gttctgaaag
     1081 taaaaaatta aatgaattga tacaaaaata tggattggaa aatcatgttt acttacgcgg
     1141 ctttttaagc aatctagacc aagaatatag cgatgcctat ttgagtttaa taacaagtaa
     1201 catggaaggt ttctctttag cgctacttga atctttagca catggtgttc cagttattag
     1261 ttatgatatt aaatatggac ctaacgaatt gattacgcct gatttcaatg gttatttaat
     1321 tactaaaaat gatgaagatg ccctatttga taaagtaaaa tatgtgattg atcatcctga
     1381 agtacaacaa cgactgtcaa aaggtagttt agcaaaagcg caacaatata gtaaagcgtc
     1441 gttaattaaa caatgggacc aatttgttcg tcttatataa atgtacttta cctttatttt
     1501 tacaaaaata gcattttcct atgtcattta actaaacatg taagttcgta tgaacgaggt
     1561 ttgttaaata gatgattcta ggaaaatgct tttttctttt gacttagttt aaaatatttt
     1621 gccactttgt actgatagta gttgcattgt actgttgtgc agattctatg ctattaatcg
     1681 aaaactgttg caatttttga gtattattta gtaattggtc gaccttttca accatttgat
     1741 tgatgtcacc ttgaggtact aaatagccat taaatccatc ttgaatcagt tctgatggcc
     1801 cataatctac atcataactg atcactggtg tacctactga aagcgactct aaaattgcta
     1861 agccaaaacc ttccatttta cttgtcgata acatcagttc tgctttagca atctcttcat
     1921 taatatgcgt cttaaaacca tgaaatttta catgttccga taaatgataa tcttctacaa
     1981 gttgtcgata ttctgacaaa ccatttccat gtccataaat attcaattga atattgggat
     2041 gttttgttac taattgcttg atcacttcaa cttgatgttt aatttgttta ttttcaacga
     2101 ggcgagcaat tgatatgata tgatttttct ccttttgatt gatgtcaaat tgatacttta
     2161 aatttgccac gtagccaacc ggaatattga taactggtat tttattttca atatattgtg
     2221 aaatatcttg gcattgctgt tctgttgata caacaatcgc tttataacgt gttaaattat
     2281 taaacactgt tttataaaaa ctttttatac cattaccggt gtcggctaaa tgtgtactgt
     2341 ggagcacaac aataactgga atactttgat ttaatcccgc tataacattt cctaattcat
     2401 gaggacgatc taatatgatt tgatcattat ttttacataa ttgatggaga aaatattgaa
     2461 ctaattcatc ttctgtatca aaaaattgtt gatgctgatc ttcatttaag ataacctttg
     2521 tgagcgtgtt tttccctttt atatcgtcga aatatttttg gatgacgatt tccccattag
     2581 gtgtatagta attttcgagt acaatccgtt gtccttcacc taaaattcga gaacaactta
     2641 aaaagcctct tccatcatac aattcgcgtt ttacttttct tcttttatga tcaaaataat
     2701 tcacatagtt taaatgatga tactgtttat ctaaaaaatg agcatacatt ataaattgct
     2761 cttcatcata tattctgaca tcatttgaat tttccacaaa tttcaatgtg tacctacatg
     2821 acttttccca atactgtatc cagttaactt gctttgtctt tttataattg attgcttttt
     2881 gaaaatagtc atacattgta aatacatcat tttcaatctg atgttgcttc gcatatgtgt
     2941 atgaataagg attccattta acatatacac attttgaaga tatgccgtgt tgtttgaaca
     3001 acttcaatct atttatttgc gctttttcta cacctgtaat tttactttct aaaattgttc
     3061 ctaaaatgta attcatatta tcgcctcata taagttttat tccgtatctt tattgtttat
     3121 tttatatgaa aaatacatct attgcatgtg taattataaa aaaaccaggc cacaaggacc
     3181 tgggtcatat tgtattattt gttttgtttt ttgcgacgac cgaataacaa taatgaacct
     3241 aatgctgcaa ataatccacc aaataacgtt gcgttatttg agccgttatt ttcactacct
     3301 gtttctggta atgcttttgc tttattgtga tggtctttag tagtactca
//
LOCUS       DAMZLE010000200         3321 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMZLE010000200 DAMZLE010000000
VERSION     DAMZLE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.204"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            22..780
                     /locus_tag="POB92_002587"
     CDS             22..780
                     /locus_tag="POB92_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6580042.1"
                     /translation="MYISILILIVFIAGCGKSDKTKEDSKEAQIKKSFEKTLDMYPIK
                     NLEDLYDKEGYRDGEFKKGDKGTWTLLTSFAKSNKPGEIDDEGMVLFLNRNIKKATGY
                     YYTSKVHDEFNEKEHQKKYHVELKNNKIVLLDNVEDSKLKNKIENFKFFSQYADFRDF
                     KNYKNGNISSADNVPSFDAEYQISNTDKNVKKLREVYPITTKKSPVLKLHIDGDIKGS
                     SIGYKNIEFNFSKVKDEETAVRDFVNFGPSDGVS"
     gene            828..1598
                     /locus_tag="POB92_002588"
     CDS             828..1598
                     /locus_tag="POB92_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6580043.1"
                     /translation="MGYLKRIGMCISLLIVIIFVTSCGGGNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFTKSNKPGELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKSILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPAKK"
     gene            1630..2421
                     /locus_tag="POB92_002589"
     CDS             1630..2421
                     /locus_tag="POB92_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6580044.1"
                     /translation="MEYLKRLALFISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKPGEMDSEGMVL
                     HLNRNMRTATGHYTIRTTYDELGKMTREKNYRVELKNNKIVVLDKVEDVNLKHKIENF
                     KFFSQYADFKDLKNYKNGRISINENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYMFSKEKEDETFMSDFLNFGPSHSN"
     gene            2473..3270
                     /locus_tag="POB92_002590"
     CDS             2473..3270
                     /locus_tag="POB92_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6580045.1"
                     /translation="MGYFKRVLLYIIVMVLSVFIIGCDKSSDTSEKSKGDSKEAQIKK
                     SFAKTLDMYPTENLEDFYDKEGYRDGKFKKGDKGTWVIRSEMTTELKNENMVSKGMVI
                     RLNRNSRTCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIIPLKPIDDEKVKKEIE
                     EFKFFVQYGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNIPTQKA
                     PKLLLKGSGNLKGSSVGYKNIEFTFVENKEENIYFTDSVYFNPSEDK"
BASE COUNT         1423 a          338 c          632 g          928 t
ORIGIN      
        1 ggatatttaa aagggtttgc attgtacata agtatcttaa ttttaatagt ttttatagca
       61 ggttgtggca aaagtgataa aacaaaagaa gattcaaaag aagcacaaat taaaaagagc
      121 tttgagaaaa ctttagatat gtatccaatc aaaaatctcg aggatttata cgacaaagaa
      181 ggctatagag atggtgaatt taaaaagggt gataagggta catggacttt actcacaagt
      241 tttgctaaaa gtaacaaacc aggtgagata gatgatgaag gcatggtttt atttttaaat
      301 agaaatataa agaaggcaac aggatattat tacacgagca aagttcatga tgaatttaat
      361 gaaaaagagc atcaaaaaaa atatcatgtt gaacttaaaa ataataaaat agttcttttg
      421 gataatgtag aagattcaaa gctcaaaaat aaaatagaaa attttaaatt ctttagtcag
      481 tacgctgatt ttagagattt taaaaactat aaaaatggaa atatatcaag tgcagataat
      541 gtaccaagtt tcgatgcaga atatcaaata agtaatacgg ataaaaatgt aaaaaaactt
      601 agagaggttt atccaatcac aacaaaaaaa tctccagtat taaaattaca tatagatggt
      661 gacataaaag gaagttcaat tggatataaa aatatagaat ttaatttttc aaaagtaaaa
      721 gacgaagaaa cagctgttag agattttgtg aattttggac catctgatgg agttagctaa
      781 agataattag atgtagaaat aattctaaaa cagagagacg tgaaacgatg ggatatttaa
      841 aaaggattgg aatgtgtata agcctgttga ttgtaatcat ttttgtaaca tcttgcggtg
      901 gtggtaataa aatcactgga gattcaaaag aaacacaaat caaaaagagt tttgcgaaaa
      961 cgttagatat gtacccaatc aaaaatcttg aagatttata cgacaaagaa ggatatcgag
     1021 atggcgaatt taaaaaaggt gacaaaggga tgtggacgat atacacagat ttcactaaaa
     1081 gtaataaacc gggtgaattg gatgatgaag gtatggtttt aaatctggat agaaatactc
     1141 gaacggctaa gggatattat tttgttaaga aattttatga aaaggataaa ttacctgata
     1201 gaaaaaatta taaagttgaa atgaaaaata ataaaagtat cttattagac aaggtagaag
     1261 atccaaatct aaaaaagaga atagaaaact ttaaattttt cggacaatat gcaaatttta
     1321 aggatttgga aaattacaac aatggcgacg tgtcaataaa ttggaatgtt ccaagttatg
     1381 acgtggaata taaaatgagc aataaagatg aaaatgttaa acaattaaga agtcgttata
     1441 acattcctac tgataaagct ccaatgttaa aaatgcatat tgacggggac ttaaaaggta
     1501 gttctgttgg atataaaagg ttagaaatag atttttcaaa agaagatagg gatatttcag
     1561 tcattgatta tttaagttat aagccagcga aaaaatagta ctgataatta aatattaggg
     1621 tgtgaaggaa tggaatatct aaaaaggctt gcattgttca taagtgttat tattttgacc
     1681 atttttataa tgggttgtga tagtcaaagc gatactgcag aaaatccaaa agaaggttca
     1741 aaagaagcac aaattaaaaa gagtttttcg aaaacgttag atatgtatcc aattaagaat
     1801 ctcgaggatt tatatgacaa agaaggatat cgtgatggcg aatttaaaaa aggtgataaa
     1861 gggacgtgga caatatcaac agattttgct aaaagcaaca aaccaggtga aatggatagt
     1921 gaaggtatgg tgctacattt gaatagaaat atgaggacgg caactgggca ttatactata
     1981 agaacaactt atgatgaatt gggtaagatg acacgcgaaa aaaattatcg tgttgaactt
     2041 aaaaataata agatagtagt tttagataaa gtagaagatg taaaccttaa acataaaatt
     2101 gaaaatttta aattttttag tcagtatgca gattttaaag atttgaaaaa ttataaaaat
     2161 ggaagaatat ctatcaatga aaatgttccg tattatgaag cagagtacaa aaggaataat
     2221 agtgatggaa atgtaaaaaa acttagagaa aagtacccaa ttacaaccaa gcagtctcca
     2281 atattaaaac tgcatataga cggagatatt aaaggtagct cagttggata taagcagata
     2341 gagtacatgt tttctaagga gaaagaggat gagactttta tgagtgattt tttgaacttc
     2401 ggaccatcac atagtaatta gtaaataaaa ccaggggcat aaactaattt aaaacaagag
     2461 agaagtgaaa agatgggata ttttaaaaga gttttacttt atataattgt tatggttttg
     2521 agtgttttta taataggttg tgataaatca agcgatactt cagaaaaatc aaaaggagat
     2581 tcaaaagaag cacaaattaa aaagagtttt gcaaaaacgt tagacatgta tcctactgaa
     2641 aatctagaag acttttatga caaagaggga tatcgagatg gaaaatttaa aaaaggcgac
     2701 aaaggtactt gggttattag atctgaaatg acaacagaac tgaaaaatga aaatatggta
     2761 tctaaaggta tggtcatacg tttaaataga aatagtagaa catgcactgg tgaatatttt
     2821 gtcaggatag ttaaagaaga cagtgagggc aaggtatata gtgatgaacg aaaatatcca
     2881 gtgaaaatgg aaaataataa aatcattcca ttaaaaccaa tcgatgatga aaaagtaaaa
     2941 aaagaaattg aagaatttaa attctttgta caatacggga atttcaaaga attggaaaac
     3001 tataaagacg gagaagtgac atataaccca gaagcaccaa tatactctgc acaatatcaa
     3061 ttgaaaaaca gtgattacaa tgtagaacaa ttacgtaagc gatataatat cccgacgcaa
     3121 aaagcgccta aattattatt gaaaggctca ggtaatttaa aaggttcatc agtcggatat
     3181 aaaaatattg aatttacctt tgttgaaaac aaggaagaaa atatttactt cacagatagt
     3241 gtctacttta atccaagcga ggataaataa gtataattat tattataaaa aatgacgtta
     3301 gaacatttaa taataaagtt a
//
LOCUS       DAMZLE010000201         3263 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMZLE010000201 DAMZLE010000000
VERSION     DAMZLE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.205"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(207..644)
                     /locus_tag="POB92_002591"
     CDS             complement(207..644)
                     /locus_tag="POB92_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDI6580046.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            complement(660..1784)
                     /locus_tag="POB92_002592"
     CDS             complement(660..1784)
                     /locus_tag="POB92_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDI6580047.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            complement(1823..2074)
                     /locus_tag="POB92_002593"
     CDS             complement(1823..2074)
                     /locus_tag="POB92_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDI6580048.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            complement(2086..2280)
                     /locus_tag="POB92_002594"
     CDS             complement(2086..2280)
                     /locus_tag="POB92_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDI6580049.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            2525..3229
                     /locus_tag="POB92_002595"
     CDS             2525..3229
                     /locus_tag="POB92_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDI6580050.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
BASE COUNT         1000 a          565 c          494 g         1204 t
ORIGIN      
        1 tgctaaaaaa tttaaaaaaa gaggttaaca catgatttga atattatgtt tgatgtccta
       61 ttaaaacagt taaatttcta gaaaatatag ttggtaaaaa cggactttat ttaacaaata
      121 gaatacaact atattctcta ttttcaatga cagacaccat ttttaatatt ataaaatgtg
      181 ttaaccttta tatttattta tgtgtactat ttacaatttt cgtcaaaggc atcctttaag
      241 tccattgcaa tgtcattaat atctctacct tcgataaatt ctctaggcat aaaataaact
      301 aaatcttgac ctttgaataa agcatacgaa ggactagatg gtgcttgctg aatgaattct
      361 cgcattgtag cagttgcttc tttatcttgc ccagcaaaaa ctgtaactgt atttgtaggt
      421 ctatgttcat tttgtgttgc aactgctact gcagctggtc ttgctaatcc agctgcacag
      481 ccgcatgtag agttaataac tacaaaagta gtgtcatcag catttacttg gttcatatac
      541 tccgatactg cttcgctcgt ttctaaactt gtaaaaccat tttgagttaa ttcgccacgc
      601 atttgttgcg caatttcttt catataagca tcatatgcat tcatatttaa ttcctccaat
      661 taaattgttc tgtttgccat ttgtttccat actgaaccaa gtgcctcatc tccgttttca
      721 atacgagata tggccatttc aatttgtaat ttaacttcaa acgcattgtc attaatatgg
      781 gcttttagtg cgggaagctg ctctgcatta ccttcatcaa agataaacat agcagcacgc
      841 cacctaacga ttttctgtgg atcatctaat agtagcacca tttctggtag tgcctctgga
      901 taccctaaat cgcttatgca atcccctgct gttcttctta cagcaggact tttatcacga
      961 agccccttat ataaatacgg taaaatttct ttactttcaa tcatacctaa taatacaatc
     1021 gcttgacgtc taaccggtac tttttcatca gataaagcta aatcaagtag cggtatatct
     1081 tcaaaagtcg gctttggaaa atggtttaac attcgtaatc gagtcttcca attatcagtt
     1141 gcatgatatt catccaaaga gacatgtcga tagaaatgat aattattagt tgcgtgattt
     1201 tcttctaaag catgttttac caatacgggt aactgtgatt ctggataggt agctagcact
     1261 tcttctagga caccatccat tacttcttca atatttccat agcgatttcc taaatctagc
     1321 catttacgca taaaaacaat attgtcatgt gctgtttgcg cttgagtcat atgatcaaca
     1381 tatgtttgtg gtaattgttc tcttaattct tggtcagcag aagttagctt aatttgatac
     1441 ggtataccct taaaagttaa taattcagct ttaatttcac caaaatgatt gtcaatttga
     1501 ggttcattta cagattctaa aaccttattc gcatcagaaa aagcagcttt aatatcaggt
     1561 aatatgactt cccaatcagc ttttggtgcc ttatcaacag ctaagaagtt catgacatga
     1621 aaaatggaag tgataccatc tatagataac aactgattta taaatcttgg ttgtgtttct
     1681 tctacttttt tataagtatt agataacttg tcttctcttg tatatgacaa aacaactttc
     1741 attgtatttg gacttggtgt tggctctata cgtaaaattt ccatatactt tattcccccc
     1801 cgaatactag ttgatgaaaa atttattcga atacagcctc tacttttggc aatactgttt
     1861 cccaatttgc gtcattttct ttatctactg aaataaagtc cataacatgg aaaattgatt
     1921 taacgccttc aacctttaag atgtcattaa taaatgctgg ctgtgaatca tcaactttag
     1981 tatacgtatc tgatgtcata ccttctctgc tttcactaag tgtaatcttc attgtgttgt
     2041 ggttcggtgt ttctgatata gatataattt tcatttgact tcctcctatt gaagttcatt
     2101 tcctaattgt tttatttctt tccctattga acactcattg atacaaaaat gatgcgcctt
     2161 cgttttcccc tctaatttac gcagacgagt tttgattgga cattgattgc aataagtatt
     2221 cattaaatca tctattttcg cgagtgcatt ttgctctgaa aaagttaaaa tacgcgacac
     2281 ttcctttatt acttcaaaat attgcaacct tgtaaataag tgttactgtt ttaaatttga
     2341 cataaaaata ttataacatt tttgacccta agatttgaat aaaacctttt acaattctat
     2401 acgtaaaatg tatttatatt caaatgctat tgcatcgcaa acgatttttc agtaaaatga
     2461 gtaagttatt tattgagcgg tttctaaaca cccgcatgat caaaatttag gaggacattt
     2521 taatatgtat aatgaaattt taggactagt tacgtttatt gcaacgttcg tacttatggt
     2581 acttatgtat cgcttttttg gtaaacaagg tttaattgca tgggttgcaa ttggcacaat
     2641 cattgccaac atacaagtga ttaaaactgt agaaatcttt ggtatttcag caactttagg
     2701 taatgtcatg tttgcttcta tttatttagc aacagatatt ttaaatgata tttatgggcg
     2761 tagagttgca aaaagagcag tttggttagg cttttcatca acattaatta tgattattgt
     2821 tatgcaattg tcattacatt ttattcctgc accagaagat atggcacaaa aagcattaca
     2881 cgcaatcttt gatgttgtgc cacgtattgc tttaggctca attgtcgcat atattattgg
     2941 tcaacatatt gatgtattta tcttttcact aattaaaaaa gtatttagtt ctgataaaac
     3001 gtttttcatc cgtgcatatg gtagtacatt tttaagctca ataattgata cagctttatt
     3061 tgtagctatc gcatttatcg gaagtttacc tggtacagtt gtatttgaaa tatttattac
     3121 aacgtacgta ttaaaattag cttcaacagt tttcaatgta ccatttggat atattgctaa
     3181 atcattttat cgtaaaggta agattgataa gttagatcaa ggctattaac gcacttacta
     3241 cacaaaagaa taagttttaa ggc
//
LOCUS       DAMZLE010000202         3250 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMZLE010000202 DAMZLE010000000
VERSION     DAMZLE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.206"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            201..926
                     /locus_tag="POB92_002596"
     CDS             201..926
                     /locus_tag="POB92_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDI6580051.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSLSNKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPKNAQIEVILEKSN"
     gene            1021..1746
                     /locus_tag="POB92_002597"
     CDS             1021..1746
                     /locus_tag="POB92_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDI6580052.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1854..2570
                     /locus_tag="POB92_002598"
     CDS             1854..2570
                     /locus_tag="POB92_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDI6580053.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KGRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(3014..3238)
                     /locus_tag="POB92_002599"
     CDS             complement(3014..3238)
                     /locus_tag="POB92_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580054.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
BASE COUNT         1213 a          494 c          478 g         1065 t
ORIGIN      
        1 gtaacgtgtc gtttttaata tataactccc gcctacttta ttaagtactg tttctgtcca
       61 aaacttaaaa atgataagtt ttgcttaaat aacactacta actgtttaag tttatttaac
      121 atagttttag cttttattta attccgaatc ggtgtaatag cttatatact ttgggtaatt
      181 cacgcaaagg agattttcat atgaaaaaga acatcatgaa taaattagtt ttatcaacag
      241 cattgttact tttaggaact acatcaacac aacttcctaa aacaccaatc agtttttcat
      301 ctgaagcaaa agcctataat atcagtgaaa acgagactaa tatcaatgag ttaataaagt
      361 attatacaca gcctcattta tcactatcaa ataaatggtt atggcaaaag cccaatggta
      421 gcattcatgc aacattgcaa acgtgggttt ggtatagtca tattcaagtg tttggatccg
      481 agagttgggg aaacattaat cagttaagaa ataaatacgt tgatatattt ggaactaaag
      541 atgaggacac agttgaaggt tactggactt atgatgaaac atttactggt ggtgttacgc
      601 cagcagctac ttcatctgat aagccttata gactattttt aaaatatagt gataaacaac
      661 aaactatcat cggtggacat gaattttaca aaggaaataa accagtatta actttaaaag
      721 aattagattt ccgtattcgt caaacattaa taaaaaataa aaagttatat aacggagaat
      781 ttaataaagg tcaaattaag ataactgctg atggaaataa ttacacgatt gatttaagta
      841 aaaagttaaa attaactgac acaaaccgtt atgttaaaaa tcctaaaaat gcacaaattg
      901 aagtcatact cgaaaaatct aactaaccta ttaccttttg taaatgcgga taatttcaat
      961 tatctaatta accccttttt ataattaaac attccaacaa tactcaaagg agaaattcga
     1021 atgaacaata acatcacgaa aaaaattatt ttatcaacaa cattgttact attaggtaca
     1081 gcatctacac aatttcctaa tacacctatc aattcttcat ctgaagcgaa agcttattat
     1141 ataaatcaaa acgaaactaa cgttaatgag ttaactaaat attactcgca aaaatattta
     1201 accttctcta acagtacgtt atggcaaaaa gataacggta cgattcatgc aacgttgtta
     1261 cagttttctt ggtatagtca tattcaagtt tatggacctg aaagttgggg caatatcaac
     1321 caattaagaa ataaaagcgt tgatattttt ggcataaaag accaagaaac cattgattct
     1381 tttgcattat ctcaagaaac gtttactggt ggtgttactc ctgcagcaac atctaacgat
     1441 aaacactata aactgaatgt gacatataaa gataaagcag aaacgtttac tggcggattt
     1501 ccagtttatg aaggcaataa gcctgtttta actttaaaag aattagattt tcgtattcgt
     1561 caaacattaa ttaaaagtaa aaaattatat aataattctt ataataaagg acaaattaaa
     1621 ataacaggtg cagacaataa ctacacaata gatttaagta aaaggttgcc atcaactgat
     1681 gcaaatagat atgttaaaaa acctcaaaat gcaaaaattg aagttatcct cgaaaaatca
     1741 aactaacaat aataatggag ttaataaaaa taatcgcaaa tactatattg acttcgctca
     1801 catttaaatt tcttattcct cgtatcatga ttcctctgaa aggagatgtt ctaatgagta
     1861 agaacatcac gaaaaatata attttaacga caacattatt actattaggt actgtattac
     1921 ctcaaaatca aaaaccagta tttagttttt actctgaagc taaagcttat agcattggtc
     1981 aagatgaaac taacatcaat gaattaatta aatattacac acagcctcat ttttcatttt
     2041 caaataaatg gctatatcaa tatgataatg gaaacattta tgttgaactt aagagatatt
     2101 catggtcagc acatatatct ttatggggcg ctgaaagttg gggaaatatt aatcagttaa
     2161 aaggtcgtta cgtagatgtg tttggactaa aagacaaaga tactgatcag ttatggtggt
     2221 cttatagaga gacatttaca ggtggcgtta caccagccgc aaaaccttct gataaaactt
     2281 ataatctttt tgtgcaatac aaagataaac tacaaacgat tattggtgcg cataaaatat
     2341 accaaggcaa taaaccagta ttaacattga aagaaatcga tttccgtgca cgagaagcgt
     2401 taataaaaaa taaaatatta tataacgaaa atcgtaataa aggtaagctt aagatcaccg
     2461 gtggcggtaa taactacact attgatttaa gcaaaagatt acattcagat ctagcaaatg
     2521 tttatgttaa aaatcctaat aaaataactg ttgacgtcct ctttgattag tatatgaagg
     2581 tgacttatac ttcatgcact ttaattccaa atcagattat ttaaatgata atttttaaag
     2641 tgtatgatgt atataatagg taaaattttc tatatattta aatggaattg ggagtaggaa
     2701 tgtgacagaa atagtatttt ataaaattta tttcgttgtc actccccaac ttgcattgtc
     2761 tgtagaattt ctttttgaaa ttctctatgt tggggccccg ccaacttgca cattattgta
     2821 agctgacttt tcgtcagctt ctgtgttggg gccccgccta taattgaaaa atgcttgtta
     2881 catgggcatt ttcattcggt caactactac caatataata ttgtagagcc taagacattg
     2941 atttattatg tcttaggctc tattccttca tttaatgatt caattattat agcaatactt
     3001 tattgtccca tgattagtgt tcttttaatg agacatagta actataaagt ttaataatcg
     3061 ttctaaatct agcatcttct agtttggttt tcccatttct taaatcttgt actgtttgat
     3121 atggaactcc tgaatttttc gaaattttat agcctgattc agactcaaac aaagattgaa
     3181 gagagtttat aatattattc aactctgtca tttttaatcc cctttcttga attaacaata
     3241 tataatgttg
//
LOCUS       DAMZLE010000203         3235 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMZLE010000203 DAMZLE010000000
VERSION     DAMZLE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.207"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(300..749)
                     /gene="ndk"
                     /locus_tag="POB92_002600"
     CDS             complement(300..749)
                     /gene="ndk"
                     /locus_tag="POB92_002600"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI6580055.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLDSAEREINLWFNENEITSYASPRDAWLYE"
     gene            complement(841..1800)
                     /locus_tag="POB92_002601"
     CDS             complement(841..1800)
                     /locus_tag="POB92_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI6580056.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSDRKEFEECIQIIRKSDS
                     INESKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            complement(1802..2527)
                     /locus_tag="POB92_002602"
     CDS             complement(1802..2527)
                     /locus_tag="POB92_002602"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDI6580057.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            complement(2530..3102)
                     /locus_tag="POB92_002603"
     CDS             complement(2530..3102)
                     /locus_tag="POB92_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDI6580058.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
BASE COUNT         1012 a          603 c          418 g         1202 t
ORIGIN      
        1 tggtttatct aaaaatatag caccatatag taattccatt aattttaaga acattaaaat
       61 ccccaaaata atcgaaataa tattcttcat tttaacaacc ccttctctct gtttagtttc
      121 atttgataaa gaatattcct ctctttttaa tacgtgttac caaaaaaact ataaaatttt
      181 tcaagtaatt tactacaaca taaaaaacgg gccttgttat gtataatcat aatgactaac
      241 cacataacaa aaccctttct acctttataa ataaatcgta aaggtttaca gtttaaattt
      301 tattcatata accatgcatc acgtggtgaa gcatagctag taatttcatt ttcattaaac
      361 catagattaa tttcacgttc agcagaatct aatgaatctg aaccgtgaat gatatttcta
      421 ccaacagtta aacctaaatc acctctaatt gatcctggtg aagcttctga aggattggtg
      481 ctgccaataa tatgtctaga tacattaact gcatcttcac cttcaactac cattgcgaac
      541 actggtgctg atgtaataaa tgaaattaaa tcattataaa atggtttacc ttggtgttca
      601 ccataatgtg tttcagcaag ttccattggt acttgcatta atttaccacc gacaagtttt
      661 agtccttttc tttcaattct tgaaattact tcaccaatta gatttctttg tactgcatct
      721 ggtttaatca ttaaaaatgt acgttccact ttatgtatcc ccctgtgttt aataggttta
      781 tgtatattac tattatatat taacaggttg gtaaagcgct ttcaatactt ttcataaata
      841 ctacgtgttt cttgaaccca tttttttcgt caaacttaaa agtagtgatt tcggatgtcc
      901 atctggtaac tcagaaatca aatccaaagc tttacttaaa tacttcgaac ttactgcttt
      961 agattcatta atactgtcag actttctaat aatttgaata cattcttcaa attcttttcg
     1021 atcgctgtca cgtcgtaatt gctcaatttt caatttgaag tctgggtttt tacgcatttc
     1081 taataaaatc ggtaacgtaa tatgaccatt aagcaaatca cttccgaccg gcttacctaa
     1141 tttcttttcg gtacttgtga agtctaatac atcatcaatg atttggaagc tcatacctat
     1201 ataatgacca atcattttca attttcgtac agtctcttta tcagattgag atgtaattgc
     1261 accaacttca gttgatattt gaattaacag tgctgttttg cgattgatac gtcgtaaata
     1321 attgataatt gtctgttgac tgttaaattg gtcttgaaat tggaaaagtt cccctctaca
     1381 aacatcaacg atagattcag atatcaattg atgtacacga ttatctttaa cggccattaa
     1441 gtgttcaagt cctaatgcca ataaaaaatt cccagttaaa atagcagttg tctgatccca
     1501 tttctttgat atggttaact tgcctcgacg cttgtcgctt ttatcaataa cgtcatcatg
     1561 aacaagtgtt gccatatgaa ttaactctaa tgcgactgca acttgatacg tttgttcaga
     1621 cgtttgttca tctttgccaa attggctact cagaattaca aatgctgggc gtactctttt
     1681 accaccagaa gacaataagt gtaatgatgc ctgttctaat acagaatctt tactttttat
     1741 tgccttttca agtcgttgtt ccactttctt aatttcattg ttcatgttta actttgccac
     1801 gttaatcacc tttggtatta tctttttctt tatagccaag gtgcattgca gcaacgcccc
     1861 ctgtaaaact acgtactctt acatttatga aacccgcttc ttcaaacatg cgcttcaact
     1921 cttcttttcc aggaaaatta aacgtagatt gctgtaacca ttcatattct tcttttgatt
     1981 ttgcaaataa tttcccaaaa ataggcataa caaatttaaa gtataatgca tacatttgtt
     2041 taaagactgg caaagttggt tggctcgttt caagacatac caccatacca cctggtttaa
     2101 gtactctatt catttctttt aacgcgacta aatagtctgg cacattcctt aatccaaacc
     2161 caattgttac ataatcaaaa gaattgtctt caaacggcaa ttccattgca tcaccatgaa
     2221 caagtttaac attttccatt gaagcagttt tttcttttcc tacttctaac atattctcac
     2281 taaagtcaat accagtaact tcacctgttg gtcctacagc tttgcttaat gcgattgtcc
     2341 aatcaccagt accacaacaa acatctaatg ctttcgtccc ttttctaacg cccatgtctt
     2401 tcatgacgcg ttttctccat actttatgct gctcaaaact aataatatta tttaatctat
     2461 catatttttt tgaaatattt tgaaaaacgc gatgtacttg ctctttattt gctttattgt
     2521 cagccatgct taattacctc tactttttaa ataacttttt tggatatcgt gtaagtaatg
     2581 ctttacttca ctttgattat atttcttgaa gtatgatgga tagtaatcag acatatcttc
     2641 aaataaataa ttatatattt ccgactcatc aatattgata ccgaaatgag ataacgtaat
     2701 atatgggaaa agtgtttcaa tttttactat tgcttgagaa atttcataat catttaaagc
     2761 ttgatgatgt aatgaagatt tcaattcatt aatttcaaca attgctttac taatttcatt
     2821 ttgaaatgat aaatcattga tttctgctag tagcgtataa aaatgtgcac taattaaatc
     2881 tccaatcaaa atggaatgtt tagacaaatg attatatgta atgtcatcaa ggtgtctcat
     2941 tgatgtgtca attgtgaggc atgctacttt ggcaacatct ggaatatcat atgaatcaag
     3001 taacttacct aaccgatgat taatattaat ggattcatat tctgatacac cctttaatct
     3061 ttcttctatt tgtctttcca atttgctaac agttgtttcc atgtctacac ctcacgatat
     3121 tatcactatt catattaaca ttatatgtaa gaaattaaaa tcttttgaag cattaagatt
     3181 acttatcatt tttaaatttc aatttaaact aacagtaatt tatgtagctt ttgta
//
LOCUS       DAMZLE010000204         3228 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMZLE010000204 DAMZLE010000000
VERSION     DAMZLE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.208"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            138..1313
                     /locus_tag="POB92_002604"
     CDS             138..1313
                     /locus_tag="POB92_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDI6580059.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1291..2481
                     /locus_tag="POB92_002605"
     CDS             1291..2481
                     /locus_tag="POB92_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6580060.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(2594..3103)
                     /locus_tag="POB92_002606"
     CDS             complement(2594..3103)
                     /locus_tag="POB92_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDI6580061.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT         1068 a          520 c          508 g         1132 t
ORIGIN      
        1 cgttttattt tgtatcaatt catctattga ttaaaattgc tataaatgta acgactttgc
       61 gtctttattt cttttataat gccgtaaaat aagttacact taaataaaat tttcaaacta
      121 atggagggtg gctattaatg gttactcaaa ataaaaagat attgattatt actggctcat
      181 tcggtaacgg tcatatgcaa gttacacaga gtatcgttaa tcaacttaat gatatgaatc
      241 tagaccattt aagcgtcatt gagcacgatt tatttatgga agctcatcca attttgactt
      301 ctatttgtaa aaaatggtat atcaatagct ttaaatattt tagaaatatg tacaaagggt
      361 tttattacag ccgcccagat aaactagaca aatgttttta caaatactat ggacttaata
      421 agttaatcaa tttattgata aaagaaaagc cagatttaat attattaacg tttcctacac
      481 cagttatgtc ggtactcact gagcaattta acattaatat tccagttgct acagtgatga
      541 cagactatcg cttacataaa aactggatta cgccgtattc aacaagatat tatgtggcaa
      601 caaaagaaac gaaacaagac ttcatagacg taggtattga tccttcaaca gttaaagtga
      661 caggtattcc tattgataac aaatttgaaa cgcctattaa tcaaaagcag tggttaatag
      721 acaacaactt agatccagat aagcaaacta ttttaatgtc agctggtgca tttggtgtat
      781 ctaaaggttt tgacacgatg atcactgata tattagcgaa aagtgcaaat gcacaagtag
      841 ttatgatttg tggtaagagc aaagagctaa agcgttcttt aacagctaag tttaaatcta
      901 acgagaatgt attgattcta ggttatacca aacacatgaa tgaatggatg gcatcaagtc
      961 aacttatgat tacgaaacct ggtggtatca caataactga aggtttcgcc cgttgtattc
     1021 caatgatttt cctaaatcct gcacctggtc aagagcttga aaatgccctt tactttgaag
     1081 aaaaaggttt tggtaaaatc gctgatactc cagaagaagc aataaaaatt gtagcaagtt
     1141 taacgaacgg taatgagcaa ttaactaata tgattagtac aatggaacaa gataaaatta
     1201 agtatgctac acagacaata tgtcgagact tacttgattt aatcggtcat tcatcacaac
     1261 cacaagagat ttatggaaag gttcctttat atgcaagatt cttcgttaaa taattacgct
     1321 aatcataaaa atttcatttt aatgcttatt atcttatttt taatggaatt tgcgagaggc
     1381 atgtatatcc taagctatat taatttcttg ccaacagtta catccattgc tgtagcaata
     1441 acgtctctag cattttcaat acattttatt gctgatgcat ctacaaactt tgttatagga
     1501 tttttactga aaaaattcgg cacaaaaatc gttttaacga ctggatttat tttagcattt
     1561 acaagtttat ttttagttat ttggtttcca gcatcacctt ttgtaatcat atttagtgct
     1621 atgatgctcg gtattgcagt tagtcctatt tgggtcatta tgttatccag tgttgaagaa
     1681 gataaacgtg gcaaacaaat gggctatgtt tatttttcat ggttgttagg cctattggta
     1741 ggtatggttt ttatgaatct acttatcaaa gtgcacccaa cacgctttgc cttcatgatg
     1801 tctttagtcg ttttaattgc atggatatta tattattttg tcgatgtaaa gttaacaaat
     1861 tataatactc gtccagttaa agctcaattg agacaaattg ttgatgtgac aaagcgccat
     1921 ttattgttgt tccctggtat tcttttacaa ggtgccgcaa ttgctgccct agttcctata
     1981 ttaccaacat atgctactaa ggttattaac gtaagtacaa ttgaatatac tgttgcaatc
     2041 attattggtg gtatcggctg tgcagtttcg atgctatttt tatcgaaact aatagataac
     2101 cgcagcagaa actttatgta tggtgttatt ctaagcggat ttattttata catgatatta
     2161 attttcactc tatctatgat tgttaatatt cacatcgtgt ggattatcgc tttagctatc
     2221 ggtctaatgt atggcatctt attaccagca tggaatacct ttatggcacg atttatcaaa
     2281 tcagatgaac aagaagaaac ttggggtgtt tttaatagta tccaaggatt cggctcaatg
     2341 atagggccat tatttggcgg attaattaca caatttacca acaatttaaa caatacattt
     2401 tatttctcgg cattaatctt tttagtctta gctgtatttt atggaagcta ctttatagca
     2461 aatagagaaa aagctaagta aggttaaaac gtattttaaa ataaagaaaa tggtcatttt
     2521 tattacattc aataaaaatg accattttct tattaattca aacatactac gattaacaat
     2581 actattatgt tacttaagct aatttaaacg tttcaataac tttccattta tcttcgtcat
     2641 cgtcaaaacg taacaaagtt aattcatcga taatttcttt atggtctacc ccagctaatg
     2701 ctacttgacc aaaaatatct tcgaattctt ggctagatag tccttgtgct attgtaaagt
     2761 gtggcacaaa aacatgttca gcttctccat agaaatcttc tccattaaag cgattaaaca
     2821 attcttctaa gtcgtccgtc ttcgcaactt taaagtaaat cacattgttc gttggtttga
     2881 agctagaagc ttttgtagca tgaacttcta ctgctggtat accattaata cgagctctaa
     2941 cctgttcaat gacagaatct aaatcaccat cttcaatttc aaatggcgct ttaattgtca
     3001 catgtggttt aattcgtgaa tactgtttat catatctttt acggtaagaa tccaccgctt
     3061 cttgaaatga ctttgatgga attaatgcta atcctaaaat catctgaatt cctcctttgt
     3121 ttaacctatt gctattatat caaatttctt tgaaaaccgt tacattaaaa ataattcatt
     3181 atacatcttt tgcaatttcg acaaatttaa attaaatcaa acagtaaa
//
LOCUS       DAMZLE010000205         3205 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMZLE010000205 DAMZLE010000000
VERSION     DAMZLE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.209"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..551
                     /locus_tag="POB92_002607"
     CDS             <1..551
                     /locus_tag="POB92_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6580062.1"
                     /translation="LSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTML
                     QFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSA
                     LANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAI
                     ILLYALGSILAVYCISHFKRAE"
     gene            555..1646
                     /locus_tag="POB92_002608"
     CDS             555..1646
                     /locus_tag="POB92_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6580063.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            1643..2245
                     /locus_tag="POB92_002609"
     CDS             1643..2245
                     /locus_tag="POB92_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6580064.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(2358..2546)
                     /locus_tag="POB92_002610"
     CDS             complement(2358..2546)
                     /locus_tag="POB92_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDI6580065.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            2685..>3205
                     /locus_tag="POB92_002611"
     CDS             2685..>3205
                     /locus_tag="POB92_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6580066.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLN"
BASE COUNT         1199 a          370 c          548 g         1088 t
ORIGIN      
        1 tgttaagttt tagtttacta acttttccat tagatattat taatgaaaaa caaaatgaat
       61 ggcgccaaag attaatggta acaccattta cttttactag ttattatatt tcaaaagtag
      121 tgaaaactat gctgcaattt gcaatagcga tattagttat ttttatggtt ggacattttt
      181 ataaaggtgt tgcaatgagt gcagttcaat ggttagagtc aggaatattt ttatggttag
      241 gtgcgtctct attaataact tttggcatat tattttcttt gttaaatgat attcaaaaaa
      301 caagtgcttt agctaatatc gtaacaattg gtttagcagt attaggtgga ttgtggtttc
      361 cgataaacac atttccaaat tggcttcaac atgttgctca tgttttaccg agctatcatt
      421 tgcgtaaact aggtgtagat attgcttcaa atcatcatat caatttaata tcatttgcta
      481 taatactctt gtatgcttta gggagtatat tagcagtata ttgtattagt cattttaaaa
      541 gggcggaata aaatatgaaa tttttaaaag atacttcaat tgctgaaata tcgtctatac
      601 tttatctgat ttttcctatt gccggtatat tttttaatga agtatatggt cccaaatggt
      661 tgtatattat atcagtcatt gtcttttcgt tgtcgtatct tatattagtt atagtaaata
      721 atagacttaa tacattaatg ttttacattt tgttgattat tcattatttt attatttgtt
      781 attttgtttt cagtgtacat ccaatgctaa gtttgttttt cttttatagt gcttttgccg
      841 ttccatttac ttttaaaaat aatgttaaaa aaacggcaac taatcttttc atactaacaa
      901 tgattatatg tacaataata acgtacttat tgtataacaa ctattttgtt gcaatgatgg
      961 tttattatgt cgttatatcg ttaataatgc tagataattt taaaaaaatg aaaaaccgtg
     1021 aatatcaaaa agaaatagca gaaaaaaata gacatattaa tacattaatt gctgaacaag
     1081 agcgacatag aattggtcaa gacttacatg atacgttagg gcatgtgttt gcaagtttat
     1141 cattaaaatc agaattagct tataaactaa tagatgctga tgtagaaaaa gtaaaagctg
     1201 aattattagc aattaataaa ttatctcgtg aatcattgaa caaagttcga gaaattattg
     1261 atgatgtaaa attaccatca tttattgaag agattgatag tatacgtaaa gttttaaaag
     1321 atgctgatat tgattttaca tttgaaaata aagaattagc gcaagtatta agtcctacta
     1381 aacaatctat gttagttatg attacgcgtg aagcgataaa taatgttatt aaacatgcaa
     1441 atgcttcaaa agttcatggt aaattaaaaa ctgtaaacaa tcataaatta ctgcttatga
     1501 ttgaagatga tggcaaaggt atcgatagtg attgtgaggt gaaaagtatt tcacagcgtg
     1561 tacaacattt aaatggaact ttagcagtcg actcaacaaa tggaactaaa ataatcattg
     1621 aaatctcaac aggaggaata gcatgacatc tttaattatt gcagaagatc aaaatatgtt
     1681 acgacaggca atggttcaat taattaaact acatggtgat tttgaaattt tagcagatac
     1741 tgataatggt ctcgatgcaa tgaaacttat tgaagaatat aatcctaacg ttgttatttt
     1801 agatatagaa atgccaggca tgactggact tgaagtttta gcggaaatta gaaaaaagca
     1861 tttgaatatt aaagtgatta ttgtaacaac ttttaaaaga ccgggatact ttgaaaaagc
     1921 agttgtgaat gatgtggatg catatgtttt aaaagaacgt tctatagaag aattggtgga
     1981 aaccattaat aaagtaaata acggagagaa agaatatagc gccacattga tgacttcatt
     2041 ttttgtagat aaaaacccat taacgcccaa agaacaaatt gtattaaggg aaattggcaa
     2101 tggtttaagt agtaaagaaa taagtgaaaa attatttttg acagatggaa cagttagaaa
     2161 ttatacatct gttataattg ataaattatt tgcagataat cgttttgatg cttggaaaaa
     2221 ggcaaatgaa aaaggctgga tctaaataca aacaaaaaag tattgagtgt tttgatttaa
     2281 gagacttgga aagtgagtca ataatcaaaa tcttactcaa tacttttatt ttggtactcg
     2341 caatttagat ttgttaacta attttttcgc ttattataaa acgaaacaat caagtaaacg
     2401 ataaagccta caaagatacc caataaaata gatagtactg ccgtcactat taatggtaat
     2461 ttgaaaaata tttgtaggaa aataccaatg ataattgcga taattactgc aattaatgtg
     2521 actgtatttt catttgaaat gttcatttat tttcactcct taacaataac attatatcat
     2581 gctatagctt tccaaaatat tgaaatatgt agatatggct attgacgata tttcttaact
     2641 tttatatgat taatcggaat gaaaaaagag aagtaggtgg caatatgaag tcaaataaat
     2701 cgcttgctat gattgtggta gccatcatta ttgtaggtgt attagcattt caatttatga
     2761 atcatacggg tcctttcaaa aaggggacga atcatgaaac tgtacaagat ttaaatggta
     2821 aagataaagt acatgttcaa agagttgtgg atggtgatac atttattgca aatcaaaatg
     2881 gtaaagaaat taaagttagg cttatagggg ttgatacgcc agaaacggtg aaaccgaata
     2941 cgcctgtaca accatttggc aaagaagcat caaattatag taagaagaca ttaacaaatc
     3001 aagatgttta tttagaatat gataaagaaa aacaagatcg ctatggtaga acattggcgt
     3061 atgtatggat aagtaaagat cgtatgtaca ataaggaatt agtggaaaag ggacttgcta
     3121 gagagaagta tttttcacca aatggcaaat atagaaatgt atttatagaa gcacaaaata
     3181 aagctaaaca acagaaatta aatat
//
LOCUS       DAMZLE010000206         3189 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMZLE010000206 DAMZLE010000000
VERSION     DAMZLE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.210"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(511..903)
                     /locus_tag="POB92_002612"
     CDS             complement(511..903)
                     /locus_tag="POB92_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580067.1"
                     /translation="MLEDNGDIRLLYQSIEELASVLGQSQFETKTVSLIFVQMDLRYS
                     VFEKVQTDFMKYLAHKNSDEIKYIDLIKIIENALPEDKELSINVINSIIIGFANNYYP
                     ILQSLVPEIQSSYGITINKNFNIDSIDF"
     gene            complement(925..1404)
                     /locus_tag="POB92_002613"
     CDS             complement(925..1404)
                     /locus_tag="POB92_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580068.1"
                     /translation="MKITKAIGSLVLTAVLLTSVSPLTNANEIQTNNTKTAHQSYENI
                     NGVKINRNPNLPMNPEAQQRIGGTKVVKTAIKLIINNRGKAKNVIQQVGGKKAATIFD
                     KGYSGIVKELKPLLKWSEVPANAVGDAVKRGAINAGLSKSDAGIISTVVKEAIAWLF"
     gene            2330..2662
                     /locus_tag="POB92_002614"
     CDS             2330..2662
                     /locus_tag="POB92_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580069.1"
                     /translation="MATLKDVKTGKKEILPSTAKDKNGKNVTLIYFEKDGKLGFYVQK
                     TQKERGVGKCVSGIAGGAVTGGTTLGLAGAGVGTVTIPVIGTVSGGVVGAVGGAVGGG
                     LTGGATFC"
BASE COUNT         1062 a          427 c          559 g         1141 t
ORIGIN      
        1 atggtcggtt tattttatag gagtaataaa aagcatttat cttttatccg ccacaaataa
       61 tgagtaaatg taccttgctt tcaatgttga tattttacaa ccttgtagag ttgatttgat
      121 atatcatctc atacgttgta ggcaatgaat acgtaaatta tgcagtgaca tgaactgttg
      181 agaagtatag agcattgaaa atcttgatat atcaatagtt ataaactggt acaaattaac
      241 gaaaagtact cttgtcatcg agtgggaata attatatata gcaaatgata ggctagaagt
      301 accgttatta cgcggtttct agcctttttt ggtgcgattg gtatgggcaa catcatgtga
      361 tgaaatgatg ggagttatat ctgtagaaat tggcgtacca atttctttat gttggggctc
      421 cgcagtcaac tactaccaat ataagattgt agagcctacg atttggattt tatgtcctgg
      481 gatcggtttc ctattcttat taaatagtat ttaaaaatct atactatcta tattgaaatt
      541 tttatttatg gttatgccat aactactttg tatttccggt actaaacttt gcaatattgg
      601 gtaatagtta ttagcgaaac caataataat ggaattaatc acattgatac taagttcttt
      661 atcttcaggt aatgcatttt ctattatttt gattaagtct atatatttta tttcatcaga
      721 atttttatgt gctaaatact tcataaaatc agtttgcact ttttcgaaaa cactatatct
      781 taagtccatt tgtacaaata ttaagctgac agttttagtt tcgaattgac tttgacccaa
      841 aactgaagca agttcttcta ttgattgata tagcaatctg atatcgccgt tatcttcaag
      901 catatgaaac actcctttaa cattttagaa taaccatgca atggcttctt tgactacagt
      961 tgagattatt ccagcatcac ttttacttag accggcattt attgcgcctc gtttaactgc
     1021 atcaccgaca gcgttagctg gtacttctga ccattttagc aatggtttca attcttttac
     1081 aatacctgaa taccctttat caaaaatagt tgcagctttt ttgccaccaa cttgttgtat
     1141 gacatttttt gctttgcccc tattattaat aattagcttt atagcagttt taactacttt
     1201 tgttccgcca attcgttgtt gtgcttctgg attcattggc aagttaggat tgcgattaat
     1261 tttcacacca tttatatttt cataactttg gtgagcagtt tttgtattgt ttgtctgtat
     1321 ttcatttgcg ttagttaaag gtgaaacaga cgttaataaa acggcagtca agactagtga
     1381 accaatagct tttgtgattt tcattataca tttatctcct ttaatagtga atttgcaaaa
     1441 ccaaatgttt gaaatataag taaataatca ttaaaaaaac aaagtgagtg aaaggtccta
     1501 attcgtataa ctagcattta ataatttaat taatcgtttt caccaaaata gaaaatgcca
     1561 cttaacacaa ttattaaaag taatgtaact atgaaagaag tccaagctaa tacaagtgaa
     1621 catattaaaa gtactaatga agtgactgtt tgtaagaacc caaaaagtaa agattccatt
     1681 atataagcaa aataaatagt tagtgaaaaa attgctacag caagtaacat ttgccctatg
     1741 attttcaaaa ttccagttgt gtagccagta actaataaat ttttcacatg aacttgatta
     1801 ccatattgcc atcccgttta taaaatagtt tagaagagat tgctattaaa tttaattgtt
     1861 ttgcataagt gtcagaatat tattcttaac tgttaagagt cgttaattta tttaaatcta
     1921 ttaagattgt tagagattat tatgcgctgg gaaacgccac tgttacagtg gaagaggaat
     1981 ttggataact agatatgtta atcgtaaatg tggcgtcgtg aaacacgact tcaacatctt
     2041 cgatcaatct atctttgaga ttggtcgaag attgaaacat atgtaagaaa acgacatagt
     2101 ttatggaaag ctcggtaagt agttgaaaaa tcaatctaga ttgaaatcat gcatatcgtt
     2161 taataaaatt tttcggagaa ataaaaactt actaaaaacg aaaaggttta tttgatttat
     2221 ttacttgtat attgtaaatg gttaaaatga gtctatagat tatgtagaga actctagtat
     2281 gccttttgag tatatgtggg attctttctt tatataaaag gagttcggag tggcaacact
     2341 aaaagatgta aagactggta aaaaggaaat tttgccatca actgcaaaag ataaaaatgg
     2401 taaaaatgtt acgttaattt attttgaaaa agacggtaaa ttaggttttt atgtgcagaa
     2461 aacacaaaaa gaacgtggag tagggaaatg cgtttctggt atagcgggtg gcgcagtgac
     2521 aggaggcact actttaggtc ttgcaggtgc aggagtagga acagttacta ttccagtaat
     2581 tgggacagtt agtggaggcg tagttggagc tgttggtggt gctgtcggcg gtggtctaac
     2641 cggtggagcc acattctgct aatagtgtag gagtgatacc atgaaaaaat tttttaatct
     2701 atgggtatgt tcgttcttat ttttagtaat aatatcatgg gtaataatta gtataaaatt
     2761 tggtaatatc agcacgatgc aaatattaat ttctaatctg tttatattta ttgcttattt
     2821 actatattta gttaagttca ttcgtttcaa aaaaacagtg gcgatcagta aacaagtata
     2881 aagaaagtcg attttgtctt tctctcgaac atatataagt ttatatagat gtagagctat
     2941 tttttatttg taaattattt tgtaattgaa aatgagtatg aacatgttct ccaatcaaca
     3001 gagtatgtct cggaagaaat ctattatact tatgtgaata agtcataatg tcagacatat
     3061 atataataag acgaatagat tgtaaatagc tttttacttt taattgttga tattccaata
     3121 ttagtaccta tatttattga agatatagtg agatttgagg atagatgtat tttaaattat
     3181 agaattgat
//
LOCUS       DAMZLE010000207         3127 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMZLE010000207 DAMZLE010000000
VERSION     DAMZLE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.211"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(98..934)
                     /locus_tag="POB92_002615"
     CDS             complement(98..934)
                     /locus_tag="POB92_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6580070.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(937..1923)
                     /gene="pta"
                     /locus_tag="POB92_002616"
     CDS             complement(937..1923)
                     /gene="pta"
                     /locus_tag="POB92_002616"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6580071.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDV
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2091..2843
                     /locus_tag="POB92_002617"
     CDS             2091..2843
                     /locus_tag="POB92_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDI6580072.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
BASE COUNT          995 a          581 c          476 g         1075 t
ORIGIN      
        1 ttaaatatta ggtcaataac atatttatgc tttttatgga tactttcaaa aataacagcc
       61 ccaaacgata acttgaaagg ggctgttaaa tatttaacta ttgcatttga tctatcattt
      121 tcttgtttct ttcaatcatt ttatcaaaat acgtatcgta tctttgccat tcttcttgag
      181 taattggcgt catatttaat acaccgccaa gatctttgat tgcatataat agtaaaaaca
      241 ttacatcttg aacagtaatc gttttgttaa gcaatgtctc taatgaggcc atacaagatg
      301 gttcaatttc aggatattta aatttagtca cttcaccttt taaagcatgt tcataaaatg
      361 tttgcatcat caatgcacgt tctgaaccag agccttcaac acaaagataa atttgtacag
      421 caataccgcc tctaactctt cgttgcgata tgcctgcaaa tttcttacca tcgatactta
      481 agtcaaattt tcctgggcaa taagaatgtt caatttccat cgtatcaata tcaacattct
      541 cattttcgaa cattttgcta attaagaggt acatcacagt aaacgcttca tcaatcgttg
      601 tttctgtttg tcctttgaac atcagcgata tatttaatac accttgatct agaacgacac
      661 ctaagccacc agaatttcta acaatggcat tataaccaat ctcattcgtt aaataatcaa
      721 tgccatcttt taaaaacggc aatcttgaat catgaatacc aagaataaca gtatgttgat
      781 gaatccaagt acgcacaaca ttatctgata tatctttgcc cacactttcg caaaatgtat
      841 catcgaatgc gaaagattgc ataggttcta atccagaaga atgatcgata tatcgccagt
      901 tgacgccatt aaaatattta ctcgctaaat ccatcgttat tgtaaggctt gcgctgctgt
      961 gataattgaa agattgtata catcttcaat tgagcagcca cgtgacaagt catttactgg
     1021 agaatttaaa ccttgtaata ctggaccaac tgcatcatat ccacctaaac gttgtgcaat
     1081 tttgtaacca atattaccag cttctaaact tgggaataca aagacatttg catcaccttg
     1141 taatttagca cctggcgctt ttttctcagc aacacctggt acaatcgcag catcaaattg
     1201 gaattcgcca tcaatgattg cttctaattt ttcttcttca gctttttgtt gtgctaattt
     1261 gacagcttct tgaacttttg tcacgtcgtc tgatttagca gacccttttg ttgaaaagct
     1321 taacattgca acttttggat ccatgccaaa gcttaatgct gattttgcac tttctactgc
     1381 aatttctgca agtccttgtg aatcaagttc tggattgatt gcacaatcac caaagatgta
     1441 ttgtacatca cctttaatca taaagaagat acctgatgtt cttgatacac ctggtttcgt
     1501 tttgatgatt tgtaaagctg gacgcacagt gtctcctgtt gaatgtgctg caccactaac
     1561 taaaccatct gctttaccag cataaacaag cattgtaccg aagtagttca cattgtttaa
     1621 taattcttgt gcttgttctt cagtcgcttt acctttacgt cgttcaacaa atgattgaac
     1681 taattcagct ttcaattcac ttgtcgcagg attaattaat tcaatattag aaatatcaag
     1741 atcaagtttt tgcgctaaag attgaacctt agtctcatca cctaacacga ttggtgtaac
     1801 ataatctgtt gcttgtaatt gtgttgcagc tgttagaaca cgttcgtcct ctccttcagg
     1861 taatacgatt ttaacgtttt taccagaaag tttgtctttt aatacattta ataaatcagc
     1921 cataatgtcc tcctgtaata taaatcttat taatcattca cggtataatt atacgccatt
     1981 ttatttcata atgccacgtc accaatttgt aatcataatt ttatcgttac ccctttgctt
     2041 tatgataaaa tttataaaga actgatgatt tttgaaaagg agcgataaac atgagtcaag
     2101 cagccgaaac attagatggt tggtatagtc tacatttatt ttatgcagtt gattgggcat
     2161 cattacgtct agttccaaag gacgaacgcg atgcacttgt cactgaattt caatcatttt
     2221 tagaaaatac agcaactgta agatcatcaa aatctggtga tcaagctatt tataatataa
     2281 ctggtcaaaa agcagatttg ttattatggt tcttacgtcc tgaaatgaag tctttaaatc
     2341 atattgaaaa tgaatttaac aaattgcgca ttgctgactt cttaatccct acatattcat
     2401 atgtatcagt cattgaattg agcaattatt tagctggtaa atctgatgaa gatccttatg
     2461 agaaccctca tatcaaagca agattatacc cagaattacc acattctgat tatatttgtt
     2521 tctatccaat gaacaaacgt cgtaatgaaa cttataactg gtacatgtta actatggaag
     2581 aacgccaaaa attaatgtat gaccatggta tgattggtag aaaatatgct ggcaaaatca
     2641 aacaatttat tactggttct gtagggtttg atgatttcga atggggcgta acattgttct
     2701 cagatgacgt attacaattc aaaaaaattg tatacgaaat gcgctttgat gaaacaacag
     2761 cacgatacgg tgaattcggt agtttctttg taggacatct tattaacaca aacgaattcg
     2821 atcaattctt tgcgatttct taatacattg gtacgtttat aaattaataa aaaaattcca
     2881 agcttatcgg tttaagcttg gaatttttcg tttatcttca gtatattccc gtatacataa
     2941 gacgtgattt ggtaaatagt tgaaatctgt atgtttaaac ttatatatat gtgctaatgt
     3001 attatcaata acaaagtaca ctttgctcat agcaaagtgt acccgagtag tcttccttgg
     3061 gagaacttta actactatca ctacatataa acgttaacct caatagaaat tatacagtcg
     3121 ctactct
//
LOCUS       DAMZLE010000208         2953 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMZLE010000208 DAMZLE010000000
VERSION     DAMZLE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.212"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(171..2780)
                     /gene="adhE"
                     /locus_tag="POB92_002618"
                     /gene_synonym="adhC"
     CDS             complement(171..2780)
                     /gene="adhE"
                     /locus_tag="POB92_002618"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI6580073.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          837 a          564 c          464 g         1088 t
ORIGIN      
        1 caatcttaat attttcttta tttaaaaaaa tgattgtcta gtttgtatct ctctgaagat
       61 ttggcaataa ataaaagccg ataaccgtat aatgattatc gacttaaagt ttatgtggca
      121 ttttttactt ttgtaatttc aggtgagtta gatgattatt atcagataga ttattgctta
      181 taatcatatg atgtttgaat gatatctttg atttcactga ttagtgcttc tttaggatta
      241 gcagttgtac attgatcttc aaatgcgagc tctgccattc tatcaattga ctcatttaat
      301 tcttcttcag acacaccttg tgatttcaaa ttcatttcaa ttccgactga ttgacctaat
      361 tcgtagacag ctttagctaa tgattctaca agtgcttctg tcgtattacc ttttaatcct
      421 aagaatttgg caatatctgc ataatctgta tctgctctga agaactcata tttagggaat
      481 aacgcatgtt tttgcgggtc tttggcatta taacggataa tatgcggtag taatatcgca
      541 ttcgctctac catgcggaat accatattcg ccaccaattt tatgcgcaat tgagtgtgca
      601 atgcctaaga atgcatttgc aaatgccata ccagccaaag ttgatgcgtt atgcattttc
      661 tctcttgaaa ctttatcacc cttttcaaca gatgatttta aatattcgaa cgtcaattta
      721 atcgcttgta gactcaaacc tcttgtgtag tctgaagcca ttacagatac atatgattcc
      781 attgcatgcg ttagtacatc cattcctgta tctgctgtaa cgctttttgg cacactcatc
      841 acaaattgag ggtcaataat tgcaacgtca ggtgttaaag caaaatcagc caacggatat
      901 tttacatttg tttcactatc tgtgataact gcaaatggtg ttacttctga acctgtacct
      961 gatgtcgtag ggatacaaat gaacgtcgca ttttcaggca tgcctatttt ataagtacgt
     1021 ttaccgatgt ctaggaactt ttgtttagca ccgaagaatg atgtctcagg gtgttcaaag
     1081 aacatccaca ttgcttttgc agcatccatc gctgaaccac caccaagtgc aatgattgta
     1141 tccggttgga aatcaaccat catttccaga cctttatata ctgtattagt tgatgggttc
     1201 ggttcgactt cgctaaatat tttaatttga ggctgttccg ttctttgtct taatacattc
     1261 tcaactgttt ttgtataacc gaattctacc ataccagggt cacagacaat catcactttt
     1321 tcaatcttgt ccattgttgt tagactcatg attgcatttt cttcaaaata aatttgagca
     1381 ggcaccttga aaatttgagt attattacgt cgtttagcaa tcgttttaat gtttaataaa
     1441 tctgtcgcac taacattatg tgaaattgag tttctaccgt acgaaccaca acctaatgtt
     1501 aaagacggaa tcaattcgtt atacatatca ccaatacctc caaccgctga tggtgtattt
     1561 acaagtacac gacaagcttt cattcttagt ccaaaatctt tttgtaatgt ttcatcttct
     1621 gtatggataa cggctgtgtg tcctaatcca ccaaaatgta gtgtgtcttc acaaatttga
     1681 aatgcttgtt ttgtagattg ggcttttact aaggctaata ctggagataa tttttcacga
     1741 gataacggat agtctgaacc tacaccgcta atttcggcta tgataagttt tgtattttcg
     1801 gggacaggta tacctgctaa ttcagctatt tcaactgcag atttaccgac aatatcaggc
     1861 ttaatacctg ttttttgttc attcataatt gcattttcta agcgttgtaa ttcatctttt
     1921 ttaacaaagt atgcttgatg tgctttaaat tcattagtaa catctttata aatttcttta
     1981 tcaatgacta caacttgttc agaagcacaa atcataccat tatcaaatgt ttttgaacca
     2041 atgatatcat ttactgcacg tttaatgtgt gctgtttttt caatgtaaga cggcacgtta
     2101 cctggtccca cacctaatgc cggtttgcca gttgaatatg cagacttaac catgcccgaa
     2161 ccacctgttg ctagaactaa tgcaatacct ttgtgattca ttaattgttt tgttgcttcg
     2221 atagaaggca cttcaatcca ctgaataata tctttaggtg cacctgcctt cattgccgct
     2281 tctaatacaa cttctgctgc acgcttcgac gattcttgtg cacttggatg gaatgcaaaa
     2341 atgattggat ttcctgtctt aattgcaatc atcgctttaa aaatagttgt cgacgtagga
     2401 tttgttgttg gcgtaacacc acaaataaca ccaattggtt ccgctacata cgttaatcct
     2461 ttttctttat cttcaccaat aatccctact gtcttattgt cttttattga attccatata
     2521 tattcagaag cgtataaatt tttaatcgct ttatcttcgt atatacctct tccagtttct
     2581 tcatgtgcta attttgctag caccatatgt tgatcaacag ctgctaagct catttgatga
     2641 acaatatgat caatttcttc ttgtgacttt ttagataatg cttctaatgc ttttttccct
     2701 ttgtcagcta gagcatcaat cataattgcc acttcttgtt ctttcgatcc acgattttct
     2761 ttttcaggta tagttaacat atacaaccac tcctttatac tttgtgaatt atttcacaaa
     2821 cattatagta catgtctctc aggatataaa gaaaattcta tgcaaaaaag tttaatttcg
     2881 aatattattt gaacaaatat caaattttaa aataaatgtt ttcatgaaat cattgttatt
     2941 tcggtgtttt tag
//
LOCUS       DAMZLE010000209         2946 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMZLE010000209 DAMZLE010000000
VERSION     DAMZLE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.213"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            299..1324
                     /locus_tag="POB92_002619"
     CDS             299..1324
                     /locus_tag="POB92_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDI6580074.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            complement(1885..2079)
                     /locus_tag="POB92_002620"
     CDS             complement(1885..2079)
                     /locus_tag="POB92_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580075.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGVVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            complement(2090..2737)
                     /locus_tag="POB92_002621"
                     /pseudo
     CDS             complement(2090..2737)
                     /locus_tag="POB92_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065796.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          971 a          418 c          445 g         1112 t
ORIGIN      
        1 gtaaaagttt taagtttaat ttgttccatt taaatcccct cttttctcta tttcctaaat
       61 catagaactt ttaacagtaa aattcaatca ttaatatatg tgttccagca tgaaaagtcg
      121 ctatgattaa aaattaatta attttcttaa tgtattgttt gatgtataat atatttattc
      181 aacttcgcga taatgcgata caaactttaa caattcacaa tacattttaa atatcgaact
      241 agttttgaaa ttagtttatc ttattaaaat gctgatatta gttttagggg gaaatgtagt
      301 gatctcattt gaaaatgatt atttagaagg tgctcatgaa aaagtattaa aacgattagt
      361 agatacaaat cttgtacaag cttcgggata tggctttgat caattcacag ctcaggcaat
      421 tgaaaagatt aaagatacaa ttgattgtcc aaatgctact atccgttttt tagtaggtgg
      481 aacgcaaacc aatcaggttg ttattaattc aatgttagaa agttacgaag gggttatttc
      541 agcagacact ggtcatgttg ctgtgcatga gggcggtgca attgaatata gtggacataa
      601 agtaattact ataccctcta aggaaggaaa agttagtgct tcagatgttg aaacttacat
      661 ggagacattt aaaagtgatt ttaaaaagga ccatatggta ttcccaggaa tggtatatat
      721 ttctcatcct acagaatatg gcacattata ctctaaatca gaattggaag agttatgcaa
      781 agtatgtaag caatatcagc ttccattatt tatggatggt gcacgattag ggtatggatt
      841 gatgagtgat caatcagata tgactattaa agacatcgct aaatattgtg atgtatttta
      901 tataggtggt acgaagatag gtgcattatg cggagaagca attgttttta cgaagaataa
      961 tgaaccgaaa caatttacca ctcgaatcaa acatcacggt gcacttttag caaaaggacg
     1021 gttaacaggc atacaatttt tagaattatt tacggataat ttatatttta atataagcag
     1081 acatgctatc gaaatggcaa ataagatgaa agacgggttt ataaataaag gctatcgtct
     1141 ttactttgat tctccaacca atcaacaatt ttttatttta agtaacgaga aaatagcaga
     1201 gttagaacaa aaagttaaat ttgctgtttg ggaaaaatac gatgatcaac atcgtgttgt
     1261 tagattcgca accagctggg cgacaaccga agaaaattta aataaactat tagaattaat
     1321 ctaataatgc tgttatattt cgtctggcaa atcatttatt aattaagcca gttcagttga
     1381 gtagttgtgt ttattgattt atatatgaaa ctactcattt gaagccgttg atgttataaa
     1441 attacatcat tccaaaacaa gatactacag attattgagt atcttgtttt attttatata
     1501 tttaaaaatg ttaatttgtg ctgtatttac tttaatttga cgagatttga aaagtattat
     1561 gatggttgta gttattttat gaaagtgatg taatatcatt tgaattacta aatctatttt
     1621 ggtaggaata tcgatgttta gtttaaatgg aatgcactat ttaagttttt aatatggaga
     1681 tgtttgtgac atttcgatga ttaagatttt tataggtgtg catctttaca aataaacttt
     1741 gtgtttaaaa ttgaacttag gaaatcgata ggtttagatg aagattttgt tgaaggtatg
     1801 tgtcttgtat ccttagttgt tataaaagcg caaaaaatgg catcacttta tctttatctg
     1861 tgtagaaagg atgctattta gaaattattt taataatcta atacttttta tctcagtttc
     1921 atcaaacgaa taaacagcaa aatcagtctg tatatctaca acaagatttc ctgtttcact
     1981 ctcaaattca ttttcatagt cagttacaac cccttcgtat tcttcattat tcaaaagcgt
     2041 gataataaca tctttacgat atgcatcttc aattctcaat acttttcacc tacttatcaa
     2101 tataaggtac tatatgagca cctgtcttcg aataatgcac ttttcctttt ctggtttcaa
     2161 tatacacatt atcgatatgt acttttccaa taatttgatt aaaattaata atctctttca
     2221 tatcaaatag ctcatcactt aatattagat tgcctgttga cattttttct cttaacaatt
     2281 cattcaataa atctatagaa agtattgtat agctaggcaa tttcttatta tttaaaatgg
     2341 ctcttttttt attttcatta tatagatgat gacctaacat atgtccattt tgtttttcac
     2401 aatttatttc tattttcatt ttacaacttt caatcatttc tttcatctgg tttttagctt
     2461 tttcttgtaa tttagcttct tttacttcta cctgatattt accttcacgc tctttaaaga
     2521 acttgtctcg ccaactgcca acatgtggca ctgtggtact tctacactaa ggatgcatag
     2581 gtggcgcatt cacacctggt atcatatctt taactttaaa tatttttccg ttaagtgaat
     2641 gacataattt agatgtttta ctatctattt tggcaacata tttatattcg ccatcttcac
     2701 caagttcttt taaatatgtt aacttttgtg attctgcatt ttcagtaaat agttaaaaaa
     2761 gcgtataaaa atagcaccac cttctcttta tctgtgtaga aaggatgcta ttgatattta
     2821 gactttgatt gattaatttc tttttctaat tcttctttct tcgataattc atccatatgc
     2881 ttaaaaaaat cttctataga aatgtaacca tctaattgtt gaattgacat aatgtattac
     2941 tcctat
//
LOCUS       DAMZLE010000210         2945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMZLE010000210 DAMZLE010000000
VERSION     DAMZLE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.214"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..204
                     /locus_tag="POB92_002622"
     CDS             1..204
                     /locus_tag="POB92_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580076.1"
                     /translation="MSNQRALLEIERNHHGYLSINFLHYHDSYKTNNKLVQIHEINPD
                     SHERIKNLIIEVLRGHRKIKKGA"
     gene            201..2156
                     /locus_tag="POB92_002623"
     CDS             201..2156
                     /locus_tag="POB92_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6580077.1"
                     /translation="MMMDIKINKLTISNFAGIKEESFNFDGKDTKIYGNNATGKTTTA
                     TALQWLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPK
                     YTINQKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLD
                     WKKRRSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIK
                     DIPIRINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGAEEINLRNQLADKQ
                     SELKRIEDNNSASNENKIHTLTNELHVENGTVANLKTRLKQNKQQIAHEENRRNQLLE
                     NHKGLKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVNEAREKALQKFNASKSKELET
                     IQTSINHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVT
                     QTDEYKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNK
                     HLDDVISELRNEEDRLLDEKEKYSHNLYILKEFTTTKVKMLTENINNEFDIAEFKLFN
                     TLVNGELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESV
                     TELIKTESQQIQLIVNEQDKKLRMETI"
     gene            2158..>2945
                     /locus_tag="POB92_002624"
     CDS             2158..>2945
                     /locus_tag="POB92_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDI6580078.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQ"
BASE COUNT         1301 a          444 c          491 g          709 t
ORIGIN      
        1 ttgtcaaacc aacgagcatt attggaaatt gaacgtaatc atcacggata cttatcgatt
       61 aatttcttgc actatcacga cagttacaaa acgaacaata agcttgtaca gatacatgaa
      121 atcaatccgg acagccacga acgaataaaa aatttaatta tcgaggtgtt aagaggtcac
      181 cgaaagatta aaaaaggagc atgatgatgg atataaaaat aaataagcta acaatatcaa
      241 actttgctgg aatcaaagaa gaaagcttta actttgacgg taaagacaca aaaatatacg
      301 gcaataatgc gactggtaag actacgactg caaccgcatt acaatggctg cttttcgata
      361 aaggtttgga cggatcaacc aaatcattta accctgtacc tttaaacgaa aaaaacgaag
      421 aaaattatga gttaattccg actgttttcg cagaatttga aatcgacgga aaaataacga
      481 ctttcaaaaa agagtcacat cctaaataca caataaatca aaaaacgaat cgcaaggaat
      541 actcacgaag tcgaacgaag aaacaatata tcaatgatga atcaataaaa gtaaaggatt
      601 ataaagctcg tattgatgaa ctgattgatg aagatgtatt caagttaatt acgaaccctc
      661 aagcatttaa cttactagat tggaagaagc gaagaagttt gttgtttgaa attgctaaac
      721 caatcaatga tgaggatgtc attaaaacaa atgatgattt taaagaatta aataatattc
      781 ttggagatca tgaaattgaa acaaagaaaa agattcttac ggacaagata aaacagatta
      841 acaaagatat caaagatatt ccgatacgta ttaaccaaac acaacaaaat aagcaggatg
      901 tacctgaatt cgataatgat agatacgcaa ttatcaaaca agaaattgag caacttgaaa
      961 atgagcgtat agatattcaa aacggtgcag aagaaattaa tttgcgtaat caattagctg
     1021 ataaacaatc agaattgaag cgcattgaag acaataacag cgcaagtaat gagaacaaaa
     1081 tccatacttt aacaaatgag ttacacgttg aaaatggaac ggttgcgaac cttaaaacaa
     1141 gattaaagca aaacaaacaa caaatcgcac atgaagaaaa tagacgtaat caattattag
     1201 aaaatcataa aggattaaaa agtgatttag aaaaagctaa aaatcaaaaa tttgaatatc
     1261 ttgatgacaa tgtatgtagt tgttgtggtc aacagttacc agctgaacaa gtgaatgagg
     1321 caagagaaaa agcattgcag aaattcaatg ctagcaaatc gaaagaatta gaaacaatac
     1381 aaacatctat caatcacatt atttcagaag gcaagaaaat aaagccaatc atcgagaagt
     1441 tagaggatga caataataat cttcaaatta aaatcaacga agcagaagag cgttcagcaa
     1501 gaatacaaaa caaaattaat aagttaaaaa cgactcacgt tgacgttaca caaactgacg
     1561 aatacaaagc agtaatgtta gagataaatg aaattaatca aaaacgctca aacatcagga
     1621 aaactattca agataaagtt tcaggaatag atgacaaaat aagcgaactt actcaagaaa
     1681 aatcagaaat tgaagtgtca agatcaatcg aaaaatcaaa taaacatcta gatgatgtta
     1741 tttctgaatt aagaaatgaa gaagacagat tattggatga aaaagaaaag tattcacata
     1801 acctttatat cttaaaagaa tttacaacaa caaaagtcaa aatgcttact gaaaatatca
     1861 ataacgaatt tgatattgct gaatttaagc tattcaatac cttagttaac ggcgaattag
     1921 aagaaacatg ttcaacaacg gttaacggcg tcgaatacga cagcggttta aataacgcct
     1981 caagaattaa tgttggctta gatatcatca atacactgtc aaaacatttt aaagttacag
     2041 cgccaatatt tattgataat gctgaatcag taacagagct tataaaaaca gaatcacaac
     2101 aaattcaatt gatagtaaat gaacaagata aaaaattaag aatggagact atataaaatg
     2161 actgaaaata ataaattaca aactattgaa caacaattag tacaagaaaa gaacgtatct
     2221 gacaacgtat taaacaaagt gagagtttta gagtcacaag gcaatttgga attgccaaat
     2281 gattattcac caagtaatgc catgaaacaa gcatggttac aaatcagcca agataacaaa
     2341 ttaatgagtt gtaacgatac aagcaaagca aatgccttat tagacatggt aacgcaaggt
     2401 ttaaatccag ctaaaaatca atgctacttt attccttacg gcaacaaaat gcagttacaa
     2461 cgtagctatc acggtaatgt aatgatgtta aaacgtgatg caggtgctca agatgttgtt
     2521 gctcaagtga tttataaagg cgatacattc aagcaagaaa tgggagaaac aggacgtatc
     2581 aaagcgatta agcacgaaca agacttcttt aacatcgaca aagaaaacat tatcggtgcg
     2641 tactgcacaa tcgtatttaa tgatggacga gataactata ttgaagtcat gactattgaa
     2701 caaattaaac aagcatggat gcagtcatca atgattaaag atgaaaaagc attacaaaat
     2761 tctaaaacac ataataattt caaagaagaa atggctaaaa aaacagttat caatagagct
     2821 gctaaacgtt atatcaacac atcaacagat agcaatcttt tcaaatacgc acaagaatcc
     2881 gaacaacgtc aacgcaaaga agtgttggac gcagaagttg aagaaaatgc aaatcaagaa
     2941 caatt
//
LOCUS       DAMZLE010000211         2929 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMZLE010000211 DAMZLE010000000
VERSION     DAMZLE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.215"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            82..>2929
                     /locus_tag="POB92_002625"
     CDS             82..>2929
                     /locus_tag="POB92_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDI6580079.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSEAENNSTTENDSTNPIKKETNTDSQP
                     EAKEESTKSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVN
                     DLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKT
                     VPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIYVN
                     PLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMR
                     TTDKYGYYNYAGYSNFIVTSNDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDAKGHYEFGGLKDGETYTVKFETPTGYLPTKVNGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTL
                     QNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEV
                     DVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSD"
BASE COUNT         1222 a          437 c          513 g          757 t
ORIGIN      
        1 atgtattacg atgggaatac aaatttttat atgtaaaaat attaattttt tgcaaatatt
       61 gatattttaa aggagattta tatgattaac agggataata aaaaggcaat aacaaaaaag
      121 ggtatgattt caaatcgctt aaacaaattt tcgattagaa agtatactgt aggaactgca
      181 tcgattttag taggtacgac attgattttt ggtctaggga accaagaagc taaagctgct
      241 gaaaacacta gtacagaaaa tgcaaaacaa gatgatgcaa cgactagtga taataaagaa
      301 gtagtgtcag aagctgaaaa taattcgaca acagaaaatg attcaacaaa tccaattaag
      361 aaagaaacaa atactgattc acaaccagaa gctaaagaag aatcaactaa atcaagtact
      421 caacaacagc aaaataacgt tacagctaca actgaaacta agcctcaaaa cattgaaaaa
      481 gaaaatgtta aaccttcaac tgataaaacc gcgacagaag atacatctgt tattttagaa
      541 gagaagaaag caccaaataa tacaaataac gatgtaacta caaaaccatc tacaagtgaa
      601 attcaaacaa aaccaactac acctcaagaa tctacaaata ttgaaaattc acaaccgcaa
      661 ccaacgcctt caaaagtaga caatcaagtt acagatgcaa ctaatccaaa agaaccagta
      721 aatgtgtcaa aagaagaact taaaaataat cctgagaaat taaaagaatt ggttagaaat
      781 gatagcaata cagatcattc aactaaacca gttgctacag ctccaacaag tgttgcacca
      841 aaacgtgtaa acgcaaaaat gcgctttgca gttgcacaac cagcagcagt tgcttcaaac
      901 aatgtaaatg atttaattaa agtgacgaag caaacaatca aagttggcga tggtaaagat
      961 aatgtggcag cagcgcatga cggtaaagat attgaatatg atacagagtt tacaattgac
     1021 aataaagtca aaaaaggcga tacaatgacg attaattatg ataagaatgt aattccttcg
     1081 gatttaacag ataaaaatga tcctatcgat attactgatc catcaggaga ggtcattgct
     1141 aaaggaacat ttgataaagc aactaagcaa atcacatata catttacaga ctatgtagat
     1201 aaatatgaag atataaaatc acgcttaact ctatattcgt atattgataa aaaaacagtt
     1261 ccaaatgaga caagtttgaa tttaacattt gctacagcag gtaaagaaac aagccaaaat
     1321 gtcactgttg attatcaaga tccaatggtc catggtgatt caaacattca atctatcttt
     1381 acaaaattag atgaagataa gcaaactatt gaacaacaaa tttatgttaa cccattgaaa
     1441 aaatcagcaa ccaacactaa agttgatata gctggtagtc aagtagatga ttatggaaat
     1501 attaaactag gaaatggtag caccattatt gaccaaaata cagaaataaa ggtttataaa
     1561 gttaactctg atcaacaatt gcctcaaagt aatagaatct atgattttag tcaatacgaa
     1621 gatgtaacaa gtcaatttga taataaaaaa tcatttagta ataatgtagc aacattggat
     1681 tttggtgata ttaattcagc ctatattatc aaagttgtta gtaaatatac acctacatca
     1741 gatggcgaac tagatattgc ccaaggtact agtatgagaa caactgataa atatggttat
     1801 tataattatg caggatattc aaacttcatc gtaacttcta atgactctgg tggtggcgac
     1861 ggtactgtta aacctgaaga aaagttatac aaaattggtg actatgtatg ggaagacgtt
     1921 gataaagacg gtgttcaagg tacagattca aaagaaaaac caatggcaaa cgttttagtt
     1981 acattaactt acccggacgg tactacaaaa tcagtaagaa cagatgctaa aggtcattac
     2041 gaattcggtg gtttgaaaga cggagaaact tatacagtta aattcgaaac gccaactgga
     2101 tatcttccaa caaaagtaaa tggaacaact gatggtgaaa aagactcaaa tggtagttct
     2161 gtaactgtta aaattaatgg taaagatgat atgtctttag acactggttt ttataaagaa
     2221 cctaaatata atcttggtga ctatgtatgg gaagatacta ataaagatgg tatccaagat
     2281 gcaaatgagc caggaatcaa agatgttaag gttacattaa aagatagtac tggaaaagtt
     2341 attggtacaa ctactactga tgcctcgggt aaatataaat ttacagattt agataatggt
     2401 aattatactg ttgaattcga aacaccagca ggttacacgc caacggttaa aaacactaca
     2461 gctgaagata aagattccaa tggtttaaca acaacaggtg tcattaaaga tgcagataat
     2521 atgacattag acagtggttt ctataaaaca ccaaaataca gtttaggtga ttatgtttgg
     2581 tacgacagta ataaagatgg caaacaagac tcaactgaaa aaggtattaa agatgtgaca
     2641 gttacattgc aaaacgaaaa aggcgaagta attggaacaa ctaaaacaga tgaaaatggt
     2701 aaatatcgtt tcgataattt agatagcggt aaatacaaag ttatttttga aaagcctgct
     2761 ggcttaacac aaacagttac aaatacaact gaagatgata aagatgcaga tggtggcgaa
     2821 gttgacgtaa caattacgga tcatgatgat ttcacacttg ataacggata cttcgaagaa
     2881 gatacatcag acagtgattc agactcagac agcgattcag actcagata
//
LOCUS       DAMZLE010000212         2924 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMZLE010000212 DAMZLE010000000
VERSION     DAMZLE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.216"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            2..987
                     /locus_tag="POB92_002626"
                     /pseudo
     CDS             2..987
                     /locus_tag="POB92_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
     gene            1208..1978
                     /locus_tag="POB92_002627"
     CDS             1208..1978
                     /locus_tag="POB92_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6580080.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            2030..2800
                     /locus_tag="POB92_002628"
     CDS             2030..2800
                     /locus_tag="POB92_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6580081.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLKPSEDK"
BASE COUNT         1197 a          319 c          550 g          858 t
ORIGIN      
        1 tatgaaaaag tgtattaaga ctttgttttt aagtatcatt ttagtagtga tgagtggttg
       61 gtatcattca gcacatgcgt cagattcgtt gagtaaaagt ccagaaaatt ggatgagtaa
      121 acttgatgat ggaaaacatt taactgagat taatataccg ggttcacatg atagtggctc
      181 attcacttta aaggatccag taaaatcagt ttgggcaaag actcaagata aagattacct
      241 tacccaaatg aagtcgggag tcaggttttt tgatattaga ggtagagcaa gtgctgataa
      301 tatgatttca gttcatcacg gcatggttta tttgcatcat gaattaggaa aatttctcga
      361 tgatgctaaa tattacttga gtgcttatcc aaagaaacaa ttgtgatgtc tatgaaaaag
      421 gactacgata gcgattctaa agttacgaag acatttgaag aaatttttag agaatattat
      481 tataataacc cgcaatatca gaatcttttt tacacaggaa gtaatgcgaa tcctacttta
      541 aaagaaacga aaggtaaaat tgtcctattc aatagaatgg ggggtacgta cataaaaagt
      601 ggttatggtg ctgacacgtc aggtattcaa tgggcagaca atgcgacatt tgaaacgaaa
      661 attaataatg gtagcttaaa tttaaaagta caagatgagt ataaagatta ctatgataaa
      721 aaagttgaag ctgttaaaaa tttattggct aaagctaaaa cggatagtaa caaagacaat
      781 gtatatttga atttcttgag tgtagcgtct ggaggcagcg catttaatag tacttataac
      841 tatgcatcac atataaatcc tgaaattgca aaaacgatta aagcaaatgg gaaagctaga
      901 acgggttggc tgattgttga ctatgcagga tatacgtggc ctggatatga tgatatcgta
      961 agtgaaatta tagatagtaa taaataagga ttcaataatg atattaagac gagtatgaaa
     1021 atagttagat tctaattatt ttcactactc gtttttattt tgaaaataag taataattca
     1081 acaatattat aaattgaaca gattgtttgt gaaatttttg ataatattaa agtgaaaaag
     1141 tgttataaat tgataaatat atgtaattaa caaaaacaaa tcattttaaa aagaagagag
     1201 ttgtaagatg atgaaacgat taaacaaatt agtgttaggc attatttttc tgtttttagt
     1261 cattagtatc actgctggtt gtggcatagg taaagaagcg gaagttaaga aaagctttga
     1321 aaaaacattg agtatgtacc ctattaaaaa tctagaggat ttatacgata aggaaggcta
     1381 tcgtgatgat cagtttgata aaaatgataa aggtacatgg attataaatt ctgaaatggt
     1441 tattcaacct aataatgaag atatggtagc taaaggcatg gttctatata tgaatagaaa
     1501 taccaaaaca acaaatggtt actactatgt cgatgtgact aaggacgagg atgaaggaaa
     1561 accgcacgac aatgaaaaaa gatatccggt taaaatggtc gataataaaa tcattccaac
     1621 aaaagaaatt aaagatgaaa aaataaaaaa agaaatcgaa aactttaagt tctttgttca
     1681 atatggcgac tttaaaaatt tgaaaaatta taaagacgga gatatttcat ataatccaga
     1741 ggtgccgagt tattcggcta aatatcaatt aactaatgat gattataatg taaaacaatt
     1801 acgcaaaaga tatgatatac cgacgagtaa agctccaaag ttattgttaa aaggttcagg
     1861 gaatttaaaa ggctcatcag ttggatataa agatattgaa tttacgtttg tagagaaaaa
     1921 agaagaaaat atatacttta gtgatagctt agattataaa aaaagcggag atgtataatc
     1981 atggctcaat cagaatatga aatcaatccc ggaaaaagag agtgatgaaa tgataaaacg
     2041 tgtaaataaa ttagtgcttg gtattagtct tctgttttta gtcattagta tcactgctgg
     2101 ttgtggcatg ggtaaagaag cggaaataaa gaaaagtttt gaaaaaacat tgagtatgta
     2161 tccgattaaa aatctagagg atttatacga taaagaagga tatcgtgatg atcaatttga
     2221 taaaaatgat aagggaacat ggattgtaaa ttctcaaatg gcaattcaaa ataaaggaga
     2281 agctctaaaa ataaaaggca tgcttttgaa gatagataga aatacaagaa gtgcaaaagg
     2341 attttactat actaatgaaa taaagacgga gaaatacgaa gtagctcagg ataatcaaaa
     2401 aaaatatcca gttaaaatga ttaataataa attcatttct actgaggaag ttaaagaaga
     2461 aaacataaaa aaagaaatcg aaaactttaa gttttttgcg caatatagca attttaaaga
     2521 tttaatgaat tataaagatg gagatatatc atataatcca gaggtgccga gttattcagc
     2581 tcaatatcaa ttaactaatg atgattataa tgtaaaacaa ttacgtaaaa gatatgacat
     2641 accaacaaat aaagcgccga agctgttgtt gaaaggtaca gggaatttaa aaggttcatc
     2701 agttggatat aaaaaaattg aatttacttt tttagagaat aaaaatgaaa atatttactt
     2761 tactgatagt ctacatctta aaccgagcga ggataaataa acgtgaccaa taaagagtat
     2821 gaaatcgaac ccggaaaaat aacaagtaat tttgtagaaa ctagcgcaac atcaaatata
     2881 tgagaatagt caaattataa gtaattaaca acgtatgtta tttc
//
LOCUS       DAMZLE010000213         2816 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMZLE010000213 DAMZLE010000000
VERSION     DAMZLE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.217"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(93..1730)
                     /locus_tag="POB92_002629"
     CDS             complement(93..1730)
                     /locus_tag="POB92_002629"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDI6580082.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNIRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(1889..2767)
                     /gene="hchA"
                     /locus_tag="POB92_002630"
     CDS             complement(1889..2767)
                     /gene="hchA"
                     /locus_tag="POB92_002630"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDI6580083.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSTLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
BASE COUNT          834 a          555 c          359 g         1068 t
ORIGIN      
        1 tgatatttct gtaaatggaa attctagttg tattaattga ttttagtaat ttatagcgtt
       61 tattattgct aattactgtc aaattaaatt ttttatccct caactcttaa actctggata
      121 tctttcatta tattagcttt tttataacca tggatatcat gtaaagcctt ataagcttta
      181 aataatgttt catacctttg tactttttcc gcttctggat taatttcgta taaaattggc
      241 tctttcatag catcaactgc atcattaatt gaattatgtg cgccaccact gactgcacct
      301 aatattgctg cgcctattgc tggtgcatac tcactatcca taacaattag ttttttattc
      361 agtacattcg catagatatc catcataaca gcattctttt taggtatacc gccacatgca
      421 aatacctttt ctacttccat atgccaatct tgatactgtt gcataatcat cttagtacca
      481 aatgctgtag cttctaaata tgctctataa atatcttcat gcttagtttg taaagttaat
      541 ccaaagatac aacctgttaa attgctatca cttaatacac ttcgatttcc attatgccaa
      601 tcaagagcaa tgagcccact ttcacctggc atttgatgtt ttatcttttc attcattaat
      661 tcaaatacag tcatatttct atttgctgct tcatctacat atgactttgg tgcttgctta
      721 gcgacatact caaacaaatc acctactgct gattgccccg cttcataagc aaataattct
      781 ggaataattg ctccttttac agaacctgat atacctggca cttgatgttg cttttcattt
      841 aacataagat ggcatgtgct tgttcccatc accatagtca tttctttatc tttttcagac
      901 ccaataccta ataaactagc atgggcatca ataataaaag gacttaccat agtctctttt
      961 gataatccta atttctgtgc cattttatca tccagtttcc ctactgcttc accaatatta
     1021 acaaccggtg cagatacttt atcttgaatt acttttgata atttggggtc tattttatca
     1081 aataaatcat aatgaaaccc tgtttcttct tcccaaaatg ctttgaaacc taatccacaa
     1141 ttcgagcgta tatttttatt agttaattta tttacaatcc aatcgcccgc ttccataata
     1201 taagccgttt tttccataat ttctggtgct cgattcatga cttccattat tttgggaatc
     1261 atccattcac tactaacatt atatccataa tggcctaacc acttattatt attttcaata
     1321 gcagtttgat ataatttctc tgcttcttta tatgcaccat gatgtttcca aagtttcaca
     1381 tacgcatgtg gattgttttt aaattgtttt aaattatgta ccgggttaag attttcgtca
     1441 gtaaaaataa tagtagatga agtaaagtct ataccaatac ctactatatt gtctggatct
     1501 atttttgatt cacgtactat atatgatatt ccttcttcca taatttctaa ataatcatta
     1561 ctattttgaa gtgcatatgt atgtggtatt ttcaaaccat ttaattcact ttcaatgaca
     1621 ccatgtgtat atggtttcac aaattttgat actacttgac cgttagttgt attaattaaa
     1681 aacacacggc ctgaagctgt tccataatca attccaatgc tataagacat catgtttcct
     1741 ccatatcgct agttgcggtt attagttttg caagcgtttt cttaaatcat actcataata
     1801 ggttctattt tacaagtatc acttaaattt tgccaaaaaa aagagccctc atacatttac
     1861 atatgaggct cctctaatta atatttaatt atttattttg tattgcattt aacatttcat
     1921 taactgctaa ttttcctaac tcatttgaag ctaaaggact gtcacctgtt aataatttac
     1981 gatcttttaa cgttcttcct gtcatatcgt cgttaactac ttttaatcct tgtttagtta
     2041 ataaatcagc aactaaccat ttcaagcgtc caggtaaata acctatttca atatttgcac
     2101 cttcatctaa tgagtcaggg aagacacaaa cagagtatcc ttctaatgta gatttttctc
     2161 tgttaagccc tgctgaaagt agtgctgctg gtccatgaca taatgtaact ataaagcggt
     2221 cattgtctaa tgcccaatct aatgtttgtt gaacatcctc actttcagaa ataccaacaa
     2281 ctgcagcatg tccgcctggg ataaagacag ataaataatc tgaatcaggt cctaattcat
     2341 ttttaatcac atctgctaat ttttttggct gttttaattt ttctttcaat ttattataag
     2401 tacttataac tgcctcgtct tcagttggca tagcccataa ttctaattta actggataac
     2461 cagataatgt cgcaacatca acgtcaaaac ctgcttccat taaatgatgt aaaggtaata
     2521 acatttcaac aggatgatta cccgtagaga acatttttcc attttccaat aatacatatc
     2581 tctcttcagc agcaatcatc aatactttcc atttaccatc tttataggca cctttgtgtt
     2641 caacaccatc aaaatctgtt ttaggtgctg tatattgact aagggaatat ggtgatggga
     2701 aaaatgcgtt atcttctgct ttatctggcg ttggttgctt acttaattca tttacatctt
     2761 gtgacatagt ttattcctac tttctctata tagttactac tagtatagac atatac
//
LOCUS       DAMZLE010000214         2783 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMZLE010000214 DAMZLE010000000
VERSION     DAMZLE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.218"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            78..2609
                     /gene="secA"
                     /locus_tag="POB92_002631"
     CDS             78..2609
                     /gene="secA"
                     /locus_tag="POB92_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI6580084.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2651..2755
                     /locus_tag="POB92_002632"
     CDS             2651..2755
                     /locus_tag="POB92_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580085.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
BASE COUNT         1030 a          397 c          559 g          797 t
ORIGIN      
        1 agtataattt tctaactata aatgataaga tatattgttg taggccaaac agttttttag
       61 ctaaaggagc gaacgaaatg ggatttttat caaaaattct tgatggcaat aataaagaaa
      121 ttaaacagtt aggtaaactt gctgataaag taatcgcttt agaagaaaaa acggcaattt
      181 taactgatga agaaattcgt aataaaacga aacaattcca aacagaatta gctgacattg
      241 ataatgtcaa aaagcaaaat gattatttag ataaaatttt accagaagca tatgcacttg
      301 ttagagaagg ctctaaacgt gtattcaata tgacaccata taaagttcaa attatgggtg
      361 gtattgcaat tcataaaggt gatatcgctg agatgagaac aggtgaaggt aaaacattaa
      421 cagcgacaat gccaacatac ttaaatgcat tagctggtag aggtgttcac gttattacag
      481 tcaatgaata cttatcaagt gttcaaagtg aagaaatggc tgagttatat aacttcttag
      541 gtttgactgt cggattaaac ttaaacagta agacgacaga agaaaaacgt gaagcatacg
      601 cacaagacat tacttacagt actaataatg agctaggttt tgattactta cgagataaca
      661 tggtgaatta ttctgaagat agagtaatgc gtccattaca ttttgcaatc attgatgagg
      721 ttgactcaat tttaatcgac gaggcacgta cgccattaat tatttctggt gaagctgaaa
      781 agtcaacgtc actttataca caagcaaatg tttttgcgaa aatgttaaaa caggacgaag
      841 attataaata cgatgaaaaa acgaaagctg tacatttaac agaacaaggt gcggataaag
      901 ctgaacgtat gttcaaagtt gaaaacttat atgatgtaca aaatgttgat gttattagtc
      961 atatcaacac agctttacgt gcgcacgtta cattacaacg tgacgtagac tatatggttg
     1021 ttgatggcga agtattaatt gtcgatcaat ttacaggacg tacaatgcca ggccgtcgtt
     1081 tctcggaagg tttacaccaa gctattgaag cgaaggaagg cgttcaaatt caaaatgaat
     1141 ctaaaactat ggcgtctatt acattccaaa actatttcag aatgtacaat aaacttgcag
     1201 gtatgacagg tacagctaaa actgaagaag aagaatttag aaatatttat aacatgacag
     1261 taactcaaat tccgacaaat aaacctgtgc aacgtaacga taagtctgat ttaatttaca
     1321 ttagccaaaa aggtaaattt gatgcagtag tagaagatgt tgttgaaaaa cacaaggcag
     1381 ggcaaccagt gctattaggt actgttgcag ttgagacttc tgaatatatt tcaaatttac
     1441 ttaaaaaacg tggtatccgt catgatgtgt taaatgcgaa aaatcatgaa cgtgaagctg
     1501 aaattgttgc aggcgctgga caaaaaggtg ccgttactat tgccactaac atggctggtc
     1561 gtggtacaga tatcaaatta ggtgaaggcg tagaggaatt agggggttta gcagtaatag
     1621 gtacagaacg acatgaatct cgtcgtattg atgaccagtt acgtggtcgt tctggacgtc
     1681 aaggtgataa aggggatagt cgcttctatt tatcattaca agatgaatta atgattcgtt
     1741 ttggttctga acgtttacag aaaatgatga gccgactagg tttagatgac tctacaccaa
     1801 ttgaatcaaa aatggtatca agagctgtag aatcagcaca aaaacgtgta gaaggtaata
     1861 acttcgacgc gcgtaaacgt atcttagaat acgatgaagt attacgtaaa caacgtgaaa
     1921 ttatctataa cgaaagaaat agtattattg atgaagaaga cagctctcaa gttgtagatg
     1981 caatgctacg ttcaacgtta caacgtagta tcaattacta tattaataca gcagatgacg
     2041 agcctgaata tcaaccattc atcgactaca ttaatgacat cttcttacaa gaaggtgaca
     2101 ttacagagga tgatatcaaa ggtaaagatg ctgaagatat tttcgaagtc gtttgggcta
     2161 agattgaagc agcatatcaa agtcaaaaag atatcttaga agaacaaatg aatgagtttg
     2221 agcgtatgat tttacttcgt tctattgata gccattggac tgatcatatc gacacaatgg
     2281 atcaattacg tcaaggtatt cacttacgtt cttatgcaca gcaaaatcca ttacgtgact
     2341 atcaaaatga aggtcatgaa ttatttgata tcatgatgca aaatattgaa gaagatactt
     2401 gtaaattcat tttaaaatct gtagtacaag ttgaagataa tattgaacgt gaaaaaacaa
     2461 cagagtttgg tgaagcgaag cacgtttcag ctgaagatgg taaagaaaaa gtgaaaccga
     2521 aaccaatcgt taaaggcgat caagttggtc gtaacgatga ttgtccatgt ggtagtggta
     2581 aaaaattcaa aaattgccat ggaaaataaa tgatataaaa taactccttc caattaaaca
     2641 cctatagttt gtgttatggg aggagtcttt ttattttaca agcgttaaat actttaaaaa
     2701 atgtgaagaa gttgttaaac gttgttatgt acttagtttt aaaaaatcgg tttaggcata
     2761 tgtcgatgat aaatgtactg att
//
LOCUS       DAMZLE010000215         2768 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMZLE010000215 DAMZLE010000000
VERSION     DAMZLE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.219"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(110..358)
                     /locus_tag="POB92_002633"
     CDS             complement(110..358)
                     /locus_tag="POB92_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDI6580086.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            466..1419
                     /locus_tag="POB92_002634"
     CDS             466..1419
                     /locus_tag="POB92_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6580087.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            1409..>2768
                     /locus_tag="POB92_002635"
     CDS             1409..>2768
                     /locus_tag="POB92_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI6580088.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKL"
BASE COUNT         1008 a          442 c          439 g          879 t
ORIGIN      
        1 ttaatcaaat taaagcaaaa taatatacac ataataataa aaagcaggtg actatcaata
       61 aacgatagct tgcctgcttt ttctatagaa catttgtcta aaaaatcaat tattcaaatt
      121 ttaaagcgtc cccatcaaat gattcgtctg caattttaat agaatctgta gggcatccat
      181 caattgcatc ttccatatct tcatataatt cctcaggtac ttctgcagta ccttggttat
      241 cgtcaaggat tacgaaagca ataccttcgt cgtcgtaatc atatatatct ggcgctgctg
      301 caccgcatgc accacatgca atacaagtat ccatatcaac gattgtatat tttgccaatg
      361 tcttcgcctc ctttgataaa aatgctaaaa tagtaatgtg actaaaattt tagacagcat
      421 catttttatt ttcaaattat ccgttttaca gagtgagggt taaatttgca acacattata
      481 aaaacagcat tacaacaaac atttaactat aaaacaaata aaagtattta caatatctta
      541 gttggtaaga aatctcacca aacctttttt gacgcttgta gtcaacaaca gttgtcatta
      601 tatcacagtt taccactatt aaaatatccg tcttttgagc tatttttaga aaaaatcaat
      661 gaatttaatg ctgaaatgga aatcatgttg catcctagat atacatttga aagcatgggg
      721 caaacatttc aagcaattca actattagtg caaaccatgt ctaataccaa acaacatgtt
      781 tttcattttg taccaatctc tcaaaataat aagatacaag aaactgtaaa gattgtctat
      841 aactacatta aagaaaacca attacaaatt gattttgaaa atgaactaca taacttgttt
      901 aaagcaataa ctttaaaagg gccatgttac ttacattatt atttgcaagg ctatgatgaa
      961 ccaatgtata cgagacagca agttagttta atagaaaagc tatctcaaca gcaattgttt
     1021 gaatacgaaa tgaataattt agtgacaatg atgtttgaat tagaaagtgg agaatatact
     1081 attttatcaa aaataataat gaaacctaca ttattaaatc aaacttatat tacttataca
     1141 aaattgcttg aacaattcac gatggaagat atagcggctc aacaacaagt taaaatcaat
     1201 actatcgaag atcatgtact tgaaatctta atcaaaggtt acatgtctaa ttacgatgat
     1261 tatgttgaac tagaagatca actccagttt ttgaattttt atcaacagca tcgtggcgaa
     1321 cgattaaaat tttacaaaga acaatttgac acgttatcat attttcaatt aaaagtatta
     1381 atcgttggat ttgaaagagg tgatctgaat gttgcatgat attttacgaa acaaatttgg
     1441 attcgagagt tttaaaccgg gacaacagga aattatagaa agtataatgt ctcaacaaca
     1501 cactctaggt atacttccaa ctggaagtgg aaagagtttg tgttatcaaa tacctacgta
     1561 tttatcaggt aagccgacat taattatctc accgttaata tctttaatgg atgaccaagt
     1621 tatgcagttg aaaataaatg gagaaaaacg tgtaacatgt attcactctg gtatggatga
     1681 aattgagaaa aagcataata ttaaatgttt acgacatagc cgcttcatct ttctaagtcc
     1741 agaatttctc ttgcaaccgt caaattttaa attaatatct atgatagact ttggcatgat
     1801 tgttctagat gaagcacatt gcctatctga atggggatat gatttcagac cacattatgc
     1861 tctaatagga aaagtaacaa agcattttaa agaagcggtt gtcttagcat tgacagcaac
     1921 tgcaccaccg catttacaag atgatttgac ggaaatgtta gcgattcaat ttaacgttat
     1981 taaaactaca atgaatcgcc caaacataag ctttaagcat cttaattttc atgatgacga
     2041 agataaaatt gagtggttgc tgccgtttct acaacagtcg ggaccaacga ttatttatgt
     2101 ctcatcgaaa aagatgtgtc tgaatttagc gcaacttatt tatgattcag gttttcttac
     2161 aggtatttat catggtgata tgaattatca agagcgacac acagttcaac aacaattttt
     2221 aaataatgat attccgatta tagtcgcaac gagtgctttt ggtatgggaa ttaataaaaa
     2281 agatattcgc acaatcattc actttcatct ttcaacaagt ccttctaact acattcaaga
     2341 aattggccgt gcgggtcgcg atggtgaact aagtcaggca attagtttat tccaaccgga
     2401 cgataaatat attttagaaa cgttattatt tgcagatatg ataacagaag aagatgtaca
     2461 aaatttcgaa ataggagaat ttttagctcc cgataaacaa gacgttttga caacgttgca
     2521 ttcattctat agtatcggcg ccttgaaaca gatatttaag caatcattta aacgaaagca
     2581 attaggattc tttcgcatga ttggctattg caaattggat caatgtagac ggaagtattt
     2641 attagaattt ttcggtgaat atccaccggc acaagatcga tgttgtgaca atgattctaa
     2701 tataactgat atcgcaattt taaataagaa gaaggtaatt agaagtattg gatttgatga
     2761 aaagttgc
//
LOCUS       DAMZLE010000216         2752 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMZLE010000216 DAMZLE010000000
VERSION     DAMZLE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.220"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            348..857
                     /locus_tag="POB92_002636"
     CDS             348..857
                     /locus_tag="POB92_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6580089.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            869..1939
                     /locus_tag="POB92_002637"
     CDS             869..1939
                     /locus_tag="POB92_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6580090.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            1956..2570
                     /locus_tag="POB92_002638"
     CDS             1956..2570
                     /locus_tag="POB92_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6580091.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
BASE COUNT         1001 a          353 c          498 g          900 t
ORIGIN      
        1 tttttaggga tgtatgtccc agcctcggcg tgtttatttt tattgacgtc taactctaaa
       61 agatataaat tagacattta taaatgatgt aaataacgca atttctatca ttgctgttaa
      121 taattcatgg tttaatatac tatgagcata aactttttaa atagtattat tcactagttt
      181 taacaatcaa ttaattggta tatgatactt ttattggtta tttttatccc atagtgtgat
      241 aattactatt tttcattcat aataaaggtt taaagcatgt taatagtgtg taagattaac
      301 atgtactgaa aaacatgttt aacataacga tataaggagt gacgtacatg atccgtctag
      361 gtaaaatgtc agatttagat caaatcttaa atctagtaga agaagcaaaa gaattaatga
      421 aagaacacga caacgagcaa tgggacgatc agtaccctct tttagaacat tttgaagaag
      481 atattgctaa agattatttg tacgtattag aggaaaatga caaaatttat ggctttattg
      541 ttgtcgacca agaccaagca gaatggtatg atgacattga ctggccagta aatagagaag
      601 gcgcctttgt tattcatcga ttaactggtt cgaaagaata taaaggagct gctacagaat
      661 tattcaatta tgttattgat gtagttaaag cacgtggtgc agaagttatt ttaacggaca
      721 cctttgcgtt aaacaaacct gcacaaggtt tatttgcgaa atttggattt cataaggtcg
      781 gtgaacaatt aatggaatat ccgccatatg ataaaggtga accattttat gcatattata
      841 aaaatttaaa agaatagagg taatattaat gacgaaaatc gcatttaccg gagggggaac
      901 agttggacac gtatcagtaa atttaagttt aattccaact gcattatcac aaggttatga
      961 agcgctttat attggttcta aaaatggtat tgaaagagaa atgattgaat cacaactacc
     1021 agaaattaag tattatccta tttcgagtgg taaattaaga agatatattt ctttagaaaa
     1081 tgccaaagac gtatttaaag tattgaaagg tattcttgat gctcgtaaag ttttgaaaaa
     1141 agaaaaacct gatctattat tttcaaaagg tggatttgta tctgtgcctg ttgttattgc
     1201 agccaaatca ttaaatatac caactattat tcatgaatct gacttaacac caggattagc
     1261 gaataagata gcacttaaat ttgccaagaa aatatataca acatttgaag aaacgctaaa
     1321 ctacttacct aaagagaaag ctgattttat tggagcaaca attcgagaag atttaaaaaa
     1381 tggtaatgca cataatggtt atcaattaac aggctttaat gaaaataaaa aagttttact
     1441 cgttatgggt ggaagcttag gaagtaaaaa attaaatagc attattcgcg aaaacttaga
     1501 tgcattatta caacaatatc aagtgataca tttaactggt aaaggattaa aagatgctca
     1561 agttaaaaaa tcaggatata tacaatatga atttgttaaa gaggatttaa cagatttatt
     1621 agcaattacg gacacagtaa taagtagagc aggatcaaat gcgatttatg agttcttaac
     1681 attacgtata ccaatgttat tagtaccatt aggtttagat caatcccgag gcgaccaaat
     1741 tgacaatgca aatcattttg ctgataaagg ttatgctaaa gcgattgatg aagaacaatt
     1801 aacagcacaa attttattac aagaactaaa tgaaatggaa caggaaagaa ctcgaattat
     1861 caataatatg aaatcgtatg aacaaagtta tacgaaagaa gctttatttg ataagatgat
     1921 taaagatgca ttgaattaat ggggggtaat gctttatgag tcaatggaaa cgtatctctt
     1981 tgctcatcgt ttttacattg gtttttggaa ttatcgcgtt tttccacgaa tcaagactag
     2041 ggaaatggat tgataatgaa gtttatgaat ttatatattc atcagagagc ttcataacga
     2101 catctatcat gcttggggct actaaagtag gtgaagtctg ggcaatgtta tgtatttcat
     2161 tacttcttgt ggcatatctc atgttaaagc gccacaaaat tgaagcatta ttttttgcat
     2221 taacaatggc attatctgga attttgaatc cagcattaaa aaatatattc gatagagaaa
     2281 gacctacatt gctgcgttta attgatataa caggatttag ttttcctagc ggtcatgcta
     2341 tgggatcaac tgcatatttt ggaagtggta tctatctatt aaatcgatta aatcaaggta
     2401 attcaaaagg tattcttata gggttatgtg cagctatgat tttattgatt tccatatcac
     2461 gtgtatatct aggtgtacat tatccaacag atattattgc cggcattatt ggtggcgtat
     2521 tttgcattat tttatcaacg ttattactta gaaataaatt aataaattaa atagtaaaaa
     2581 aacaaaagca gtaaacctaa agtgtcgtaa gggtttactg cttttataaa acgttgttat
     2641 aacgtatatt gtcttttacg ggcatataaa aggggaatat ttgaaaatga ccaatccaac
     2701 aagaacgaaa cgttgtgggg ggatgttcta tgtggtattg ataatcattt tc
//
LOCUS       DAMZLE010000217         2715 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMZLE010000217 DAMZLE010000000
VERSION     DAMZLE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.221"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..424
                     /locus_tag="POB92_002639"
     CDS             <1..424
                     /locus_tag="POB92_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6580092.1"
                     /translation="PNDITKDFLLSLLKSKFENNRQTFDKIYNILNIEAIPSNVLLKN
                     LSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLNDFIQSHQTKTIINI
                     THNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            430..1161
                     /locus_tag="POB92_002640"
     CDS             430..1161
                     /locus_tag="POB92_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDI6580093.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNTLFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTYMCPTEIINTVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1323..1943)
                     /gene="srtA"
                     /locus_tag="POB92_002641"
     CDS             complement(1323..1943)
                     /gene="srtA"
                     /locus_tag="POB92_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDI6580094.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            2137..2628
                     /locus_tag="POB92_002642"
     CDS             2137..2628
                     /locus_tag="POB92_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6580095.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
BASE COUNT          886 a          443 c          387 g          999 t
ORIGIN      
        1 tcctaatgat ataacaaaag attttttatt atcattgtta aaatcaaaat ttgaaaacaa
       61 tcgacaaaca ttcgataaga tttataacat actaaacatc gaagcaatac cgtctaacgt
      121 actactaaaa aacttgagtg atggtcaaaa acaaaagctt aaattattaa gtttcttatt
      181 ggaagatcat gatttaatca tattagatga agttacaaac gctttagaca agaaaacagt
      241 taatgaaatt tatgaatttt taaatgattt tattcaaagc catcaaacta aaactattat
      301 caatattaca cataatttat ccgatttaag tgctttgccg ggtaaatact ttatttttaa
      361 agaccttcaa atagaagagt accaatcaaa agaagaagtc ataaatgatt acattaattt
      421 ataatttagg tgtccacttt gaaattagaa tttaaaaaaa gtatttccaa taaaattatt
      481 tacacgcttg gtgtgctatt catattcctt ttcttacttg ggtatttttt accaatagga
      541 atagacaaag ttaaaagttt aagctatagc cagtttttct tcagttcata tactgttgca
      601 acgcaattag ggttcttact attttcattt gttattgcat attttattaa taaagagtat
      661 tcgaataaaa ataccctgtt ttataaattg attggcgata atatctttac cttcttctat
      721 aaaaaggtag cagtgttatt ttttgaatgt ttagtgttca taattctaag tatcacaatt
      781 atttcaatta tatattcgga tttctcacat tacttactat taataatctt attttcactc
      841 gttatcttac aatatatctt agttgttgga acgattagta tggtttcacc taatatttta
      901 atcagcttag gtatcagcat tgtatattgg attggttcag tcatcttagt tgccattaat
      961 aaaaatatat ttggtatcgt tgctccattt gaggcgagca atacgatgta tcgtgccgta
     1021 gaaaaaatat tgaataacga gtcaacatat atgtgtccta ctgaaattat aaatacagta
     1081 tcattcttcg ttcttctatt tatagttaat actattgtct tattactaag tagaaagaga
     1141 tggttgaaaa tcggtatgta gtacataaaa aggagcggca tccattgtcg ctccttttta
     1201 ttatgtattt ttacaatctc caccaatgac acgtcgtcat tgctacctca taccaaacaa
     1261 aaaaacagtc tcgcaatcaa gactgttttc cactcaatat attcatccat tagcgtaata
     1321 gattatttga cttctgtagc tacaaagatt ttacgttttt cccaaacgcc tgtcttttca
     1381 ttgtaatcat cacaagtaat taatgttaat tgtttatctt tacctttttg ttcatctaga
     1441 acttctacat ctgtaggctt aacatctctt atacttgtca ttttatactt acgtgtttca
     1501 ttaccaactt taaagtacac catactacct tttttggctg ctttaagatt tgtaaattga
     1561 tagttcggac ggtcaatgaa agtgtgtcct gcaattgaaa tattttgatc atctagtgat
     1621 tcgttttctt ctgcaaagct tacacctcta tttaattgtt caggtgttgc tggtcctgga
     1681 tatactggtt ctttaatatc agcatctgga atttcaatat agcctgccac tttcgattta
     1741 tctttcggaa tttgaggttt agcttgctgc tttttatctt tactcgcctg ttcttttaca
     1801 tttttatcat attgttcaat cttttcatct ttatctttat cgtgaagata attatcgata
     1861 tgtggtttag caaacaaata tgctgccact aggataagta ccacaccagc gattgtcatt
     1921 aatcgatttg tccatttttt catacgttaa ggctcctttt atacatttca caatgcctgt
     1981 taacatttca tatagtatac cattaatttc aaaatgactc atagcaattc attttatact
     2041 ataaaattta catgtatact tttacgttag atttcattac acatatttgc attcaaataa
     2101 cgaaacgctt ttaataatta ctaaggggga attgatatga ttagatacgc taaaaaagag
     2161 gatttaaacg ctatattagc gatatacaat gatgccatta tcaatactac agctgtttat
     2221 acttatgaac cacaaaccat agacgaacgt gtcgcatggt ttgaaacgaa acaacgtaag
     2281 catgagccta tctttgtatt tgaggaaaat ggaagtgtct tagggtttgc gacgttcggt
     2341 tcgtttagac cttggccagc atacctatat acaatcgaac attctattta tgtcgatgct
     2401 tcagctagag gaaaaggtat tgctagtcaa ttactacacc atttaattgt tgaagcaaaa
     2461 gctaaaggtt atcgtgcgct agttgcaggc attgatgctt ccaacaaagc gagtattcag
     2521 ttgcatcaaa agtttgcttt taagcatgcc ggcacactga ccaatgtagg ttttaaattc
     2581 aatcaatggt tagatttagc attttacgaa ttagatttac aagactagta atgtttgaat
     2641 cacataatat aaacaagaca accatgttaa ttcccttaac ataacaagcc aacatataaa
     2701 attttaaact tctca
//
LOCUS       DAMZLE010000218         2685 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMZLE010000218 DAMZLE010000000
VERSION     DAMZLE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.222"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(2..147)
                     /locus_tag="POB92_002643"
                     /pseudo
     CDS             complement(2..147)
                     /locus_tag="POB92_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226109.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            complement(320..922)
                     /locus_tag="POB92_002644"
     CDS             complement(320..922)
                     /locus_tag="POB92_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6580096.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(937..1113)
                     /locus_tag="POB92_002645"
     CDS             complement(937..1113)
                     /locus_tag="POB92_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDI6580097.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1312..2298
                     /locus_tag="POB92_002646"
     CDS             1312..2298
                     /locus_tag="POB92_002646"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI6580098.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(2379..2597)
                     /locus_tag="POB92_002647"
     CDS             complement(2379..2597)
                     /locus_tag="POB92_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDI6580099.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1008 a          396 c          390 g          891 t
ORIGIN      
        1 gttatttttt attgcttaaa tttattattg ctactactat accaatgaaa gtgacgataa
       61 acataccgaa acttaacatt aaatgtaatg cgtttgcaat agatatcaca ataggctttt
      121 cctttcaaaa gtgttaagta atgccatagg caccaccccc ctttactcag atagccacca
      181 tcaattcaac ttgcccacaa attattatac tatttttata aaatattacc actataatga
      241 agcatgcctc gaatcttcgt attgctattc ttaagcaaaa aagcttttct tagaacacat
      301 acgtctaaga aaagcttatt taagcattaa aaattaataa caatataata gctacaattt
      361 gtgcaacgga tgttgttgct atacgtcgag tatactcata acttaatgat aatatcactt
      421 gtgcaacaaa tataaatgtt actatccacc aattatctaa ataaataaat aatgaactag
      481 aacaaattgc agttattaat atagctaccc atttcggtat tgaaaaatgt gttaattctt
      541 tttgtaaaac agttctcata tataactcgt gacatggaat gacaaaaagt aaagttacta
      601 acatttgcca cttccaattg acacctgttc ttgctaattc tttaattaat gaactataac
      661 ttaaattaga agttataagt gagaataaaa gttgaagcat aattaaaata attgctgtaa
      721 taataccaac accaattgat gtaagcaacc gcttagattc aatatctctt tgataaaata
      781 cataactgat accagcaatt agcattatac ctgtatataa ataccagtat tcttggtgtt
      841 cgccccacat aataaataat atgagatgaa atataatgaa acttataata aaccaagtta
      901 aagccttcga gattttattc attataactt accttcttat tcaacaatct tatatcgttt
      961 atgattgata acagtttttt ccggtaaatc aaggtcatta aaattttcca taattgttaa
     1021 gtcaacaata acagagttat cgttgatttt ttcaacacga cctcttaatc catccataaa
     1081 ctcaacaatg ttacccactt ctgcaacagt cattttaagt cctccttaaa tcataactaa
     1141 attatattgt actaaaattc acttaacaaa taaactatta acaatcaaaa ataataaaat
     1201 gtaactttaa gccgatattt agaatgattt gtgagtatat aattggcatt ttgagcaaag
     1261 ttaaggcata aataagtaga gaatatatat agatataagg gagagattgt aatgaaattc
     1321 attagtaata ataatattac agatccaact ttaaacttag caatggaaga atatgtttta
     1381 aaaaatttac cagcagaaga aagttacttt ttattttaca taaatagacc atctatcatt
     1441 gttggaaaga atcaaaatac gatagaggaa gtaaatcaga catatataga tgctcataac
     1501 atcgatgtag taagaagaat ttctggtggt ggcgctgttt atcatgatac tggcaattta
     1561 aactttagtt tcataacaga tgatgatggt aacagtttcc acaatttcca aaaattcact
     1621 gaaccaattg ttcaagcatt acaatcttta ggcgtcaatg ctgaattaac tggtcgtaac
     1681 gatattcaag ttggacaagc gaaaatctca gggaatgcca tggttaaagt taaaaataga
     1741 atgtttagtc atggtacgtt gatgttaaat agtgatttag acgaagttca gaatgcacta
     1801 aaagttaatc cagctaagat taaatcaaaa ggtattaaat ctgttcgtaa acgagtagca
     1861 aacattcaag aatttttaaa tgacccttta gaaattgaag aatttaaaaa aatcatcttg
     1921 aaaactatct ttggagaaac tgaagtagaa gaatataaat taacggatga agattgggaa
     1981 aatattgaaa agttaagtaa cgacaaatat agaacttggg aatggaatta tggtagaaac
     2041 cctaaatata actttgaacg cgaggaaaaa tttgaaaaag gatttgtaca aattaagttt
     2101 gatgttaaac gaggtaaaat cgaacatgca aaaattttcg gtgatttctt tggtgtcgga
     2161 gatgtcactg atcttgaaaa tgcattagtt ggctgtctac acgactttga gcacatagaa
     2221 gaagcactat cagaatatga tttatatcac tatttcggtg acatagatag acatgaatta
     2281 attagattaa tgtcataaac gattaaagga ctattcttac atgtatgaga tgttaagaat
     2341 agtcctttta tgattgcgtt atggaattcg aatcaatgtt attcaccgag aaatgcctcc
     2401 aaatttttgt attctgccgt atattgatta aatgctgctt catctttatt gacgagtgcg
     2461 tcatctataa gttctatgag ctgtcgtttt cttacctctc gaagtgcttc attgataatt
     2521 aattcaatac ccaaatcatt gacggttgtg acaaacgctt ctaaagttgt atgtttaaca
     2581 ttagtattgt atttcataga aaccactacc tttctttaat ttataataag tatacatttt
     2641 tctaattttg aaatcaatga atattctgac tattagttaa aaatt
//
LOCUS       DAMZLE010000219         2654 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMZLE010000219 DAMZLE010000000
VERSION     DAMZLE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.223"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            70..435
                     /locus_tag="POB92_002648"
     CDS             70..435
                     /locus_tag="POB92_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDI6580100.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            617..1894
                     /locus_tag="POB92_002649"
     CDS             617..1894
                     /locus_tag="POB92_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDI6580101.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2165..2521)
                     /locus_tag="POB92_002650"
     CDS             complement(2165..2521)
                     /locus_tag="POB92_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDI6580102.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT          908 a          391 c          440 g          915 t
ORIGIN      
        1 tggtaaaaac tctttattaa ataaattgat tcgttcatac tgaatagata agcaaaaagg
       61 agttacataa tgaagaagaa aaaaaacgcg attaaagtta tcattatatt atttatttca
      121 ttaattgttg cagtagcatt tttctttggt ctgaaaacat atcaaggcca taagaatatt
      181 caactaattg acagttactt ggaagagaaa aatctaaaag ataaaattaa atcagaaaaa
      241 acagagtata gtgctaaaaa aggtctcttc tataaggaag ttacatttaa agatgaacct
      301 ggtgtaactt atgttgtgca accaatcagt actaataaag gattatttgt tgaaggcttt
      361 gatacagaga ctaagaaaag tttgaaaact gcaaagcata aatattttaa tcaaaattat
      421 aaaccatcaa aatagtgtaa cgtataactg gtgaaacagt ttagtgcctt caattttaat
      481 tatttgaggt aaacagtttt tgtatggaaa ttagtgattt ttgacatcta taaaagataa
      541 atattttaat tttttataga taattatcaa ttttctgata taattgagtg atgtcagatg
      601 aaggggagtt tgtactatgg ctctattcaa gagaaaaatt agcctaccta tgcaagttgt
      661 tattgcatta gtgttaggtg ttgtcgtagg acttttatta tatggacaag aaaacgtagc
      721 aaattacatt aaaccatttg gtgacgtatt tttaaattta attaaaatga tcgttatacc
      781 agttgtattt tgctcactag cgctttctat ttcgaacgtt ggggaatcga aaactgtagg
      841 gcgttatggc tggaaaacaa ttttatactt tgaaattatt acaacaatcg caataggttt
      901 agggattatc ttcggtaacc tatttaaacc aggtgctgga ttagacccaa caaaattacc
      961 taaaggtgat atttctaaat atcaatcaac tgcacatgca gcagaacaat ctacatatgg
     1021 aaatcatttt attgatacca ttgtacatat tattccgaca aacttttttg aagctttaaa
     1081 taagggtgaa ttattaccta ttatcttctt cgcagtattc tttggattag gattagctgc
     1141 tgtaggtaaa aaagcagaac cagttaaaga atttttaagc ggatcgcttg aagctgtgtt
     1201 ctggatgatt aataaaattt taaaattagc accacttgga gtgtttgcat tcatttgtac
     1261 tacaattatt acatttggtg catccgcatt attaccacta ttaaaattag tattagttgt
     1321 tgtctttgca atggtgttct ttgtattcgc tatactagga ctagttgcat ggatgtgtgg
     1381 tattaatatc atgaatatta ttagaatctt gaaaagtgaa ttgcttttag cattttctac
     1441 atcaagttcg gaagctgtac ttcctgtaat gatgaagaaa atggaaaact tcggttctcc
     1501 aaaagaaatt acttcttttg ttataccaat tggttatacg tttaacttag acggatcagc
     1561 actttatcaa tctattgcag cattattcgt tgcacagatg tatggaatgc acttaacatt
     1621 atcagagcaa attgtgttga tgttaacatt aatgattacg tctaaaggta tggcggcagt
     1681 accaggaact tcaatcgttg ttttattaac aacattaggt gccatgggct taccggcaca
     1741 aggtttagca ttaattattg gtgttgaccg tatcttagat atggtacgta catgtgtaaa
     1801 cgttattggt aacgcattat caacaatcgt tatagctaaa tgggaaaacg tatatgacaa
     1861 agcaaaaggt caagaatatt taaaatcaat ttaaaaaata ctatctgaca tttaaagccc
     1921 cttacaacct ttggttgtta ggggctttta tgcatgcgca aaagcgcagg cgaaccgaag
     1981 cgcagtgacc tcaggttgtt aggggctttt atgcatgcgc aaaagcgcag gcgaaccgaa
     2041 aatagactga cacagtaatt cttaaaaagc agcagtaaga tgattccaaa ttggaaatca
     2101 acttaccgct gcttttactc gtatatacct tgatggcaac taaaatttgt aatttaaatt
     2161 acaattagtg tttcatgtgc attttgccca tgtcatgaat tttcatcatt aaaccgtgag
     2221 ggacgtcatc atggtcaact tttttaactt taacaatttt atctgaatct gctaatttat
     2281 aaacaattac aaaatcacct tttttaactt taacatctga atctgaaggt aatttaacat
     2341 ttttttcaat gctttcatct ttttcactaa tcattttttc aatagtagta ttatctttca
     2401 taattccata gtatgtatct ttctttccgc cagtcatcat catgattact aatacaatac
     2461 atacaattaa catgatggcg attaaactaa taccaagacc aaaacctttt ttctttttca
     2521 tggttcatca atctccttaa atgtagttaa ttctgttata cctaaaagtg caacagtttg
     2581 caaatgcagt atttatatta cataaaatta atttaaaaag acacacccta atcacgttaa
     2641 atgtgaataa agtg
//
LOCUS       DAMZLE010000220         2616 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMZLE010000220 DAMZLE010000000
VERSION     DAMZLE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.224"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(36..1190)
                     /locus_tag="POB92_002651"
     CDS             complement(36..1190)
                     /locus_tag="POB92_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009385210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6580103.1"
                     /translation="MPFVIYVLGLLIFAQTTSEFMVAGIMPVLSEEFGVSISSIGYLI
                     SAYSAGMVIGGPILTLALLKVARKKALLTLTFVYLIGQILGAMAPNYEIMFVARLITG
                     ISSAACFGLCMAIAFQLVSAHTRGRAASIVLGGLMLATAAGLPITIMVEQYFGWRASF
                     WTIALLVLLAGILAYIFIPSVAQQEQTSVRKELGLFKNYSLWAAYVTSMVVIGAIITG
                     FSYFTPILTSISGVKSTTVPYILAGYGIAMVIGNVIVGRLADTYMISVLVVGLSVLTV
                     TFVVFGLFTELPMIAVISIITVGFVGLSMNPALAIRVTRVGGTGTLVASVHNSMINLG
                     NTFGPAIGGFAIEAGFGLASPLWVGLILSIIALISLLPIVRGNIPFLNTK"
     gene            1559..1903
                     /locus_tag="POB92_002652"
     CDS             1559..1903
                     /locus_tag="POB92_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6580104.1"
                     /translation="MLDEQSKYRIVTTLQAIGSKWKPYILFILLVKGTKRFGELRHLI
                     PEITQGVLTQQLRDLERDGLITRVVYHEIPPKVEYSLSKHGKSLEPVLSAMCDWGFIH
                     EEFIKGIRKERK"
     gene            complement(2193..2381)
                     /locus_tag="POB92_002653"
     CDS             complement(2193..2381)
                     /locus_tag="POB92_002653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580105.1"
                     /translation="MQKLTVEETRKIEGGINWPERGIFGDSVYNTGKHVGAANKCWND
                     GKRGLDFVRCQQATFNGK"
BASE COUNT          973 a          471 c          380 g          792 t
ORIGIN      
        1 cagaaccatt atttttctat agatatgttg accacctatt tagtattaag aaaagggata
       61 ttacctctaa caataggtaa taaagatatt agagcaataa tagataaaat taaaccgacc
      121 cataatggag aagctaatcc aaatccagcc tcaatagcga acccaccaat cgcaggacca
      181 aatgtattcc ctaaattaat catcgaatta tggacactgg caactaatgt acctgtacca
      241 ccaactctag ttacacgaat ggctaaagca ggattcattg ataatcctac aaaaccaaca
      301 gtgattattg aaattacagc aatcatagga agttctgtaa aaagtccaaa taccacaaag
      361 gtaactgtca agacgctaag tcctacaact aaaactgaaa tcatataagt atcagccaat
      421 cgacccacaa taacattccc tattaccatt gctataccat aaccagcaag aatataagga
      481 acagttgtcg atttaactcc cgaaatacta gttaaaattg gtgtaaagta actaaatcct
      541 gtaataattg cacctataac taccatactt gttacataag ccgcccataa agaataattt
      601 ttaaataaac caagttcttt tcgaacactg gtctgttctt gttgagcaac agatggaata
      661 aagatatatg ctaatatgcc tgctaataaa actaataagg caatagtcca aaagctagcg
      721 cgccatccaa aatattgttc taccataata gtaattggaa gaccagctgc ggttgccagc
      781 attaaaccac ccaaaacaat ggaagcagca cgtccgcgag tatgcgcact cactaattga
      841 aaagcaatcg ccatacatag accaaaacaa gcagcagaac taattcccgt aattaaacgt
      901 gctacgaaca ttatctcata attgggtgcc atcgccccaa gtatctgacc tattaaatac
      961 acaaaagtaa gagtcaacag ggcttttttt cgagccactt taagtaaagc taaggtaagt
     1021 atggggcctc cgatgaccat acctgccgaa taagctgaaa taaggtaacc aatagaagag
     1081 atagatacgc caaactcttc cgaaagaact ggcattatac cagcgaccat aaattctgat
     1141 gttgtttgtg caaaaataag taatcctaat acataaatta caaagggcat ttaatcaact
     1201 ccaatttcaa atatttacct aaaagtacga aaaatgatag aacactgttc attacaagat
     1261 gaaataaatg gaaggtatat tgtttcttta tatgtacaaa gtattttgta ctttataata
     1321 attatgccga tctaaattca gattgaaaag tagacatatc tttcatactt agtatgttaa
     1381 aaggtactaa ttcaatcacg tacgatataa taactcctat tttttgttaa attatccata
     1441 tatacaaaac aagaaatcta tcaggctaat ttattatttt tgttcttttc atttgatatg
     1501 attaatatac ttagtacgaa tttatgatta tacattgtta ttttaaaggg gagatactct
     1561 gttggatgaa caatcaaaat atagaattgt taccacgctt caagcaattg gtagtaagtg
     1621 gaagccttat atattattta ttcttttagt aaaaggcaca aaacgtttcg gggaattgag
     1681 acatttaatt cctgaaataa cacagggtgt actaacgcaa caacttagag acttagaaag
     1741 ggatggccta atcactcgtg ttgtttatca cgaaatccct ccaaaagtag aatattcttt
     1801 atctaaacat ggaaaatcat tggaacccgt attaagtgct atgtgtgatt ggggatttat
     1861 ccatgaggaa ttcataaagg gaatacgaaa ggaacggaaa taaaagttat acatagatat
     1921 atttgaacta ctcccccatt gatgagaata taatcaaaat actttttttg gttctgttgc
     1981 aaagttaaaa atcatgtaat atttatactg aaaaacgaaa aataattaat actaacaaaa
     2041 ctctttactg ttaaaacaat atttgctttt atagttttgt atagatgtgc atataatttt
     2101 aactttgcaa cagaacccta ttttttaata aaaaacagtc caaacgtctc atgcctaggc
     2161 aggggacgtt tggactgaga ataccacttt tcttatttac cattgaaagt agcttgttgg
     2221 catctcacaa aatccaagcc tcttttacca tcattccaac atttgttagc tgccccaacg
     2281 tgtttcccag tattataaac ggaatcccca aaaatacctc gttcaggcca gttaattcca
     2341 ccttcaattt ttctagtttc ttctacagtt aatttttgca tcttcaataa ctcccttata
     2401 attatttaat actatcgtag ccatctgaaa aatctttacc atactgatct acaaataaaa
     2461 tagtaggacc tacaacaggt aattttttaa atagactttt caaaatattc accccttttt
     2521 ttgatttaaa ttaatagtag caaaaaatta ttaaaaaaaa gtaaatctaa gaataagatt
     2581 atttttaggt ttataaaagg aaatttattc tttaag
//
LOCUS       DAMZLE010000221         2610 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMZLE010000221 DAMZLE010000000
VERSION     DAMZLE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.225"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..699
                     /locus_tag="POB92_002654"
     CDS             <1..699
                     /locus_tag="POB92_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580106.1"
                     /translation="SDTSQEAQEIQEAKREAQAETDKSVAVSNEESKAVALKVSKEES
                     QAERLANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSESET
                     VNIDNKDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKAS
                     TNKTSGQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKV
                     EKANSKIEKRTFND"
     gene            855..938
                     /locus_tag="POB92_002655"
     CDS             855..938
                     /locus_tag="POB92_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580107.1"
                     /translation="MLFVTSLDYNSDIAHFKLKMEKYSIIK"
     gene            1399..2490
                     /locus_tag="POB92_002656"
     CDS             1399..2490
                     /locus_tag="POB92_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDI6580108.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT         1057 a          353 c          445 g          755 t
ORIGIN      
        1 agtgatacat cacaagaggc acaagagatt caagaagcta aaagagaagc acaagcagaa
       61 acagataaaa gtgtagctgt atcaaatgaa gaatcaaaag cagtggcatt aaaggtatcg
      121 aaagaagagt cacaagctga aagattagca aacgctgcaa aacagaagca agctaaatta
      181 acaccaggtt caaaagagag tcaattaact gaagcgttat ttgcagaaaa accagttgct
      241 aaaaatgact tgaaagaaat tcctcaatta gttactaaaa agaatgatgt atcagagtca
      301 gagacagtta atatagataa taaagacact gttaaacaaa aagaagctaa atttgaaaat
      361 ggtgttatta cacgtaaagc tgatgaaaaa acaactaata atacagctgt tgacaagaaa
      421 tcaggtaaac aatctaaaaa aacaacacct tcaaataaac gaaatgcatc aaaagcatct
      481 acaaataaaa cttcaggtca gaaaaagcaa cataataaga aatcatcaca aggtgcaaag
      541 aaacaaagta gttcaagtaa gtcaactcaa aagaataatc aaactagtaa taagaattca
      601 aaaacaacaa atgctaaatc atccaatgca tcaaaaacgc caaatgctaa agttgaaaaa
      661 gctaatagta aaatagagaa acgtacattc aatgactaag attactaaaa tcgtttaata
      721 atcaatcata gcagtaagct atgtattata tttttaaaaa ttgaatttca aaataaaagg
      781 taggcattca agtgcttacc ttttttgata tcatcagttt attttgaaag gcaacgcgat
      841 cattccatgt atttatgttg tttgtaacat cgttagatta caatagtgat attgcacatt
      901 ttaagctgaa gatggaaaaa tattcgataa tcaaataaac aatgaatctt agaaggtaca
      961 atgacgttca ctaatttaaa tatagctgaa tgtgttggtg agtgatgttc actatagatt
     1021 tatattaata tacaaagaca aaggttgtta atttttatta agcgttaggt tgaatgtatg
     1081 agaattttag atttataata gaagatagaa acgaaaattt ttcttaaaag cagtaatgtt
     1141 gactcaaaat aagctataat aatgacactt atttaattga ttaacatttg ctaataaata
     1201 tcaatataga atataacttt ccaataatga ctgagaaaat cgaaatgtca gtctcgaatc
     1261 atataattag aaaattgatt attttctgtc aatttagggt tgaactatac atatgatatt
     1321 gttagaatat tttttaacat tatattttat tgctttaaag tggaatatac ttgaaataat
     1381 tagtagaggt gagtaaggat gagtaataaa ttagaatcat acagaagtga gattgtatca
     1441 ctgaatcatc aaattttaga cttattatct aaacgtggtg aactagcaca aaaaattggg
     1501 gaagaaaaat taaaacaagg tacacgtatc tatgatccac aacgtgaaaa agaaatgctt
     1561 aacgacttaa tcgatagtaa caaaggacca ttcaacgata atactattaa gcaattattt
     1621 aaagaaattt tcaaagcctc tacagattta caaaaatctg aaaatgaaaa acatttatat
     1681 gtatcacgta agttgaaacc tgaagatacg attgtaacat ttgataatgg gggcattatt
     1741 ggagacggca ataaatcatt tgtatttggg ccatgttcag ttgaatcatt tgaacaagtt
     1801 gaagctgttg ctaaaaactt acatgctaaa ggtgaaaaat ttattcgtgg cggtgcattt
     1861 aaaccacgta catcaccata tgatttccaa ggcctaggtg ttgaaggact taaaatactt
     1921 aaacagatta aagataaata tgatttaaat gttgtcagcg aaatcgtaaa tccaaatgat
     1981 tttgaagtgg ctgatgagta tttagacgta ttccaaattg gtgcacgtaa tatgcaaaac
     2041 ttcgagttat taaaagaagc tggccgtacg aaaaagccta ttctattaaa acgtggttta
     2101 tctgctacaa tcgaagagtt tgtttatgca gctgaataca ttgcttcaca aggtaatcaa
     2161 aacattattt tatgtgaacg tggaatccga acttatgaaa aggcgacacg taacacttta
     2221 gatatttcag cagtaccaat tttaaaacaa ggtacacact taccagtcat ggtagatgta
     2281 acgcatagta caggtcgtaa agatatcatg ttaccaactg cgaaagcagc attagcagtt
     2341 ggtgctgatg gagttatggc tgaggtgcat ccagatccat ctgttgcact tagtgatgcg
     2401 ggtcaacaaa tggatttaga tgaattccaa gcattttatg atgaattaaa gcctttagct
     2461 gatttatata acgctaaaaa gttaaaataa tattccaagg aaactataga ctacttaact
     2521 aatatgtcat gttgaagtag aatattatct ttgaatcgac aattttaaac ttacagccat
     2581 tctaagagta tattactttt agagtggcta
//
LOCUS       DAMZLE010000222         2573 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMZLE010000222 DAMZLE010000000
VERSION     DAMZLE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.226"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..530)
                     /locus_tag="POB92_002657"
     CDS             complement(<1..530)
                     /locus_tag="POB92_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580109.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPS"
     gene            complement(544..1335)
                     /locus_tag="POB92_002658"
     CDS             complement(544..1335)
                     /locus_tag="POB92_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI6580110.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(1516..1719)
                     /locus_tag="POB92_002659"
     CDS             complement(1516..1719)
                     /locus_tag="POB92_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580111.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(1748..2557)
                     /locus_tag="POB92_002660"
     CDS             complement(1748..2557)
                     /locus_tag="POB92_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6580112.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          797 a          464 c          356 g          956 t
ORIGIN      
        1 atagatggta tatcaaagaa atcttggctt aaaaatgcat gttctaaata caaaaataat
       61 aaatctgtat attgtgtttt agtacgatac actttaattt gagattccgt atatgatata
      121 cgtgtatcta agcgaagatc aattaattta gcagtacttg ggcgctcaac tttaatagaa
      181 tttaatacgc gcatatcttc taataattta aaaagttgtt gataaaattt agggtgttta
      241 aaagttttat cttgtactac ttcatttaca atttgtacat ccatcatatg ataaccgtaa
      301 gcagctaaca taacatctgt ttttaaacca gccatttcga tatggcttgg tcgatgtgac
      361 caaaaccaac tcgtaatcaa aacattttga acaggattat agtaagggaa tttttggatt
      421 ttaacttgtg tttgttcatt ttttaataag agtcgagcta agtcttgcaa catcattact
      481 tgcttggcat ctaactgttc gtcattaaat tttatgaaac gatcactcat gttttatccc
      541 tctttaaaag tttagttcta cagcattata tattgcttgt tgttcacgtt catcttccaa
      601 tttatcaata attgtacgtt taattgcacg ttcaactggc attacagtga ttaaatcaca
      661 caagtctaat aatgcacgga tactagcggc ttcttcagaa atttgtccct gcttagacat
      721 agtacgtaaa tcttcattaa acttaataat ttgttcgatt tgtttatcat cttgtaataa
      781 actttgctct ttaatcacat tttttaaaat gtccccatca atataatcaa cgtgaataac
      841 aacaaagcga ttttttagtg cttcattcat tggcaaagta ccaacataac cttcatttat
      901 cgctgctata acgttaaatc ctggtacagc tttgattact tcaccagtgt atggattcgt
      961 aatttgacga cgataatcta agaccccatt taatacaggc aatgtttcag gtttagccat
     1021 atttatttca tcaatatata aaatatgccc ctctttcata gctttaataa ctggaccatc
     1081 tacaaagaca atttcttgtt gaccttccgc atttgtttta attgttttaa agcctaataa
     1141 gctttctgta tctaaatcaa cagaacaatt gacttgatgc atgggtgtat caacaacttc
     1201 acttaatgtt tctgccaact ttgttttccc tgaacctgtt ggacctttaa gtaaaatatt
     1261 tttatttaaa tcaaataatg ccttcgcatc attgaaaact gttgaatctg aattcttata
     1321 atgttttagt gccattttaa aacgttctcc tttaaataaa ttaaaaaact aagacatgga
     1381 ataagttctc caaatgcctt agtttactat tctattctga tataaaacgc aatgcttagt
     1441 atgcgaattg tttatatttt accaaattta ctaattgaaa atctcatatt aggcttcatt
     1501 tcaataatta tgcgtttaaa cttctttgaa atatgtttta tagtaaccgc caactaatcc
     1561 agaattatct attatttgat aatattcttc agtttcgttg ataacatcat attgtttgcc
     1621 aaccgttaac atgtcagaaa ctgtaaattt ctttgcatct gtattaatga cttctacttt
     1681 cttgattggt gtacgttctt tccaagtttc atgtaacata aattttgtcc tcgctatcgt
     1741 aaaaaaatta tttttgtaaa gtgaatataa acgataatcc actgttttca gtaattctaa
     1801 ttgattcaat atcatcttgc tcaagcgttt gatatggtat ttccacttca tctaagcgcg
     1861 atttcgcttg attaaagaaa tctgcatctt tcgtttcaaa ctcaacttga tcgactgcgc
     1921 catattccgg cattgtcatt tcggtttcta caggcattaa atgtatttcg ccacctagac
     1981 cacccgttcc aactttgaat acttggacat gatagtcagc attgtcgaag ggttggtatt
     2041 ctgcaaatac ttcaagtccg aatatatttg ttaaaatttg acctgtaata tctacatgat
     2101 tcactttaag aatcactggt cctaaacctt gcacttgatg taacggattg accgcactct
     2161 caaaagaagg catacctaaa ccaacgccat aattattctc gtttgaatat attgagaaaa
     2221 tatgaccatt gttatcttcg agactgaaat atttattgcc atttaattct ttaactgttg
     2281 taaatggaat atccttattc gataatattt ccgcatactc ctctaaacct gagtcagttg
     2341 gtgtacgtaa tccaataccc acaaagtgag attcttctaa ttgctcactt ggaacttgta
     2401 taaattgaag tcttgttcct gggcttatat ctgcatcgcc gaaacgaatt gagttttcaa
     2461 gaagttcttc ataatttaat cctaatatgt caaccatgaa atgttttgtc tgttctatat
     2521 ttgttgtacc taatgttata cttctaagtc cacacattat agccacctct atc
//
LOCUS       DAMZLE010000223         2567 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMZLE010000223 DAMZLE010000000
VERSION     DAMZLE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.227"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            452..556
                     /locus_tag="POB92_002661"
     CDS             452..556
                     /locus_tag="POB92_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580113.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKSKI"
     gene            685..936
                     /locus_tag="POB92_002662"
     CDS             685..936
                     /locus_tag="POB92_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580114.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            1009..1845
                     /locus_tag="POB92_002663"
                     /pseudo
     CDS             1009..1845
                     /locus_tag="POB92_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503679.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            2038..2166
                     /locus_tag="POB92_002664"
     CDS             2038..2166
                     /locus_tag="POB92_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580115.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYVSWRKMKVILLT"
     gene            2133..2345
                     /locus_tag="POB92_002665"
     CDS             2133..2345
                     /locus_tag="POB92_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580116.1"
                     /translation="MEKNESNITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKER
                     IARERKKSDERIKDAVQEAEKLAKMK"
BASE COUNT         1047 a          309 c          434 g          777 t
ORIGIN      
        1 catatggtga atggatatta taataattaa aatacaaaaa tagtagattc caatttgtga
       61 aaaaggagta tgtcgtttca tttagaattt attgcacgaa attagcatcg aataagggaa
      121 aataagtgtt aagttttaaa tgataaaaaa gattggaatg gatcgtcttg aaatgctccc
      181 ttcaaagttt tcattttttc aatgtctact tcgaaggggc atttccatta tattgttata
      241 gctttttata tttgtataat gaacatataa gtttaagaag atgagagtga aggaaataaa
      301 aaagctcaaa tgtaccaaat tgttaatctt aataaatctc tactttataa agattgaatg
      361 gacattcgag cgttaatcag tcaggaggga atttcactcc tacaatttaa taataatact
      421 tgcttcacca ctgtacaagg agtgatttgt tatgttcaaa gtgaattatt cgattttaag
      481 ttattatcca gaatataata tcgcagtaag ttggcaacgt ttaagagaag gaaaaacaat
      541 aaaaagcaag atttaatact gctgcgtcat gaggcgcttg aacattattt gatgaataag
      601 tataatttca actatgatta tgcacataaa attgtatcaa aaaaatacga ttattcaatt
      661 tttataaaaa agaaggtgga ttaaatgctt acattaataa aattgaaaga agatgaacag
      721 gttataatat atgaatatat acctgaagat gatataagta acggtaaagg ttcagtaact
      781 tttaataaaa aagatgcaga ggttatagat ttctcattat ctgaaataga aaatgaagaa
      841 tattttatgt tatatcgtaa taagtctttt tctgtagtaa gagactttat cgagaagcaa
      901 gaatttcccg aaaattataa aatagcatgg tattaatgca tcctttctag acagataaag
      961 agaaagtggt gctattttta tatgcttttt taactattta ctgaaatagc agaatcacaa
     1021 aagttaacat atttaaaaga acttggtgaa gatggcgaat ataaatatgt tgccaaaata
     1081 gatagtaaaa cgtctaaact atgtcattcg ctcaacggca aagtatttaa agttaaagat
     1141 atgataccag gtgtgaatgc gccacctatg catccttggt gtagaagtac cacagtgcca
     1201 catgttggca attggtgaga caagttcttt aaagagcgtg aaggtaaata tcaagtagaa
     1261 gatgacacaa ccaaagatga attacaacaa gctaaagtat taggaaagaa aatatatatt
     1321 actgatcaag caattgataa agtcagatat gttgatattc caacacatac caaagaagaa
     1381 aatcaattta tacaagaaca acataaagcc ttgcttaaag atgccaaaga aaacaatgat
     1441 agcaatgaag tagcttattt attaaaagat ggtaaagtta caaaagtata tggtgatcaa
     1501 gatagtgtat cttttgcacc aggggaaaaa gcaacagaat tgttatttaa cagtaaaccg
     1561 aattcaattg ttatgttaca taaccatcct gggcagtcta gtttttctct tacagacttg
     1621 tatttattta tatttaataa ttctattaaa acactgacaa ttgttacaaa taaaggtcaa
     1681 acaaagtact taactaagac aaaagaatat tgcaaatcaa cttgtattga ttgtattaaa
     1741 aaatataata aaaataagaa tataaaaaaa ttcaatcata aggatattga tatgattcta
     1801 aagagattat ataatagtgg taacataata tataaagtta ggtgattaaa atgtattttg
     1861 cacagttaga tggtgaaata actaataaac agtcacaaga attattggat aaagaataca
     1921 agaaagcaat agaattagaa aataagaata aagaacaaaa attagaaaaa tagcatcctt
     1981 tctacacaga taaagagaaa gtggtgctat ttttatacac ttttttaacc ttccaatgtg
     2041 aaggttattt tttattgtcc aaaacgtgct gatgacattt taaaagcaag tatggaatat
     2101 cagtcgacag actataaacg gaggtatgtc tcatggagaa aaatgaaagt aatattactg
     2161 acgtaactca gaacgaagag caactagaca acagtgatga acaatcacaa cagaatgaga
     2221 aaacattttc tcaagaagaa gtatcacaat tgattaaaga gcgtatagct agagaacgca
     2281 aaaaatcaga tgaacgtatt aaagatgcgg ttcaagaagc tgagaagtta gctaaaatga
     2341 agtagcttat ttattaaaag gtgctaaagt tacaaaagta tatggcgatc aagatagtgt
     2401 atcttttgtg ccagggtaac aagcaaccga attattattt aacagtaaac cgaattcaat
     2461 tgttatgtta cataaccatc ctggacaatc aggattttca ttgaatggtt taaagatgtt
     2521 tattggaaat aaatctatcc aaacaacgac tattgttact aactata
//
LOCUS       DAMZLE010000224         2565 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMZLE010000224 DAMZLE010000000
VERSION     DAMZLE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.228"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            32..2182
                     /locus_tag="POB92_002666"
     CDS             32..2182
                     /locus_tag="POB92_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6580117.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHHTFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNY
                     LKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
     gene            2328..>2565
                     /gene="sarX"
                     /locus_tag="POB92_002667"
     CDS             2328..>2565
                     /gene="sarX"
                     /locus_tag="POB92_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDI6580118.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEK"
BASE COUNT         1026 a          296 c          389 g          854 t
ORIGIN      
        1 gtggtactta aatatagaaa gaggtaaagt tatggcaaaa tcatgcttgc atatacttac
       61 taataatgaa tatgcgacaa cgcgttgcca agatggcata gtcttatttt ggccaattga
      121 cggggaaatc gaactacaaa aatttcgtaa aagtaaaata attgaagatg atatatatat
      181 tattaatcat ctggatgtat ttagtattaa gaataataaa aaaacgatca tgttgtattt
      241 gagtagcgat tggtttgcgg aattaggctt tactttcttt aattaccact atacagcaaa
      301 gttgattaaa tcatcctata atttgaaatg tctactatta aaattgacat atcgatacct
      361 tgataatcag cctcttaatg acgctgatat tagaaaatta caggatatta ttaaaatcat
      421 tgcaaaagaa gcaagtatgg ataaaaagat tgcacaaaat caatatcgat atgcttatta
      481 tggtgatttg cgtgatgagc tcgaatatat ttatcaaaat gtaaatcaac gattgacatt
      541 aaaaagtgtc gctgataaat tatttgtctc aaagtcaaat ttgtcatcac aattccactt
      601 acttatgggc atgggtttta aaaaatatat tgatactttg aaaattggta aatcgattga
      661 aattctactt actactgata gtactattag caacataagt gaacatttag gttttagtag
      721 tagctccact tactctaaaa tgtttaaaag ttatatggat ataacaccga atgaatatcg
      781 taatttatca aaatataata aatgtttaat gctaaagcca gaaccactag taggcaaaat
      841 ggtgcaagaa gtaaaagaaa tcatattgaa ttatattgaa cattataaaa accacctaac
      901 tgatgttata catattgatg aagacaaatt tgaaacacct aaattgtttc aaacggttat
      961 tcaaataaat acttatacag aaatgaaatt agttttctta gaaggaatct ttaaaacctt
     1021 attgaataag aacagtcaag ttgtcttttt catcatgcca tcgattctaa aaagtaaaaa
     1081 taccatgtcc gaagaagaaa aattcacaat cattaaaaca ataattgaaa gtgatctaaa
     1141 gatagcattt aatataaatg atattgaaac aacttatttt gttgaagaag cttttatgag
     1201 tgttttcaga caaatatctc caaacgaatt aagtaatcat aataattacg aagtgcattt
     1261 tgtttttgat ttatcattga tggaaattag aacaatttat cgaatgatat taaaattaca
     1321 taacatcatg ttgaatgtga aattaggatt gaacattacc tgtttatttg aaaaaccttc
     1381 agtttttaaa tcactagtat cacaaataaa gcgacttaaa ttcgattcgt taataataga
     1441 taatgcaaat ttaagtagcc cttatttgat gggggaaagt gatgagttac tattgaaaaa
     1501 tattttgcat tttaaaaatt taaaacaagt aattaatgaa ttggatattg aacaagaaaa
     1561 gcttattttt ctaaatgttg aaaatcataa actgcttaat aataaagaac gagatttaag
     1621 taatagtgct ccattaattt ataagacatt aagtgcgctg tatcacaact ttgatggctt
     1681 tggattaaac atttttgata atcatcatac atttaatgcg atgcatctat atgataaaaa
     1741 tggatttaaa acaacactag gtcttatatt ggaaaaattt atcgaatatg tctcgaaacc
     1801 aaaatacgaa aacagttatt attctatttt tgatatagag aattattatt gtcttgttat
     1861 ttatgattgg cgagtgatag agagcgagac aattatgagt aattttgagg atagtcaagt
     1921 ttatataaat tttaaaaaca atgttttaaa cgataaatat ctaattgtaa tagaaacatt
     1981 ggacgaaaat agtggcaaca ttaatcattt gatttctaaa gaattaagag ataaatatga
     2041 atggaaccct agtttactat ctaaaattga caactacctt aaaccagcaa tagagattaa
     2101 agagcataat tttagtgata attccttgaa tattaacgtt acttttaatg cgttatacat
     2161 aattaaaata ggaaaaaaat aacaccttga tatgtattgc aaaatttaat ttgcatttgt
     2221 tggtaatttt gacaacgtat taaaaacaaa tgagaaagac aatggcaaaa aaatttaaga
     2281 taataatgac agaagcattg tctttatata tttgggggtg caacattttg aatactgaga
     2341 aattagaaac attgcttggc ttctataaac aatataaagc attatctgaa tatattgata
     2401 aaaaatataa gttgtcgcta aatgatttag cagtcttaga tttaacgatg aagcattgca
     2461 aagatgaaaa agtacttatg caatcatttt taaaaactgc aatggatgag ctagatttaa
     2521 gtaggacaaa attattagtt tctataagaa gactaattga aaaag
//
LOCUS       DAMZLE010000225         2549 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMZLE010000225 DAMZLE010000000
VERSION     DAMZLE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.229"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(201..284)
                     /locus_tag="POB92_002668"
     tRNA            complement(201..284)
                     /locus_tag="POB92_002668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Leu,seq:caa)
     gene            complement(413..487)
                     /locus_tag="POB92_002669"
     tRNA            complement(413..487)
                     /locus_tag="POB92_002669"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(452..454),aa:Gly,seq:tcc)
     gene            complement(495..568)
                     /locus_tag="POB92_002670"
     tRNA            complement(495..568)
                     /locus_tag="POB92_002670"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(534..536),aa:Cys,seq:gca)
     gene            complement(574..645)
                     /locus_tag="POB92_002671"
     tRNA            complement(574..645)
                     /locus_tag="POB92_002671"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(611..613),aa:Gln,seq:ttg)
     gene            complement(657..729)
                     /locus_tag="POB92_002672"
     tRNA            complement(657..729)
                     /locus_tag="POB92_002672"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:His,seq:gtg)
     gene            complement(732..805)
                     /locus_tag="POB92_002673"
     tRNA            complement(732..805)
                     /locus_tag="POB92_002673"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(771..773),aa:Trp,seq:cca)
     gene            complement(821..901)
                     /locus_tag="POB92_002674"
     tRNA            complement(821..901)
                     /locus_tag="POB92_002674"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(865..867),aa:Tyr,seq:gta)
     gene            complement(907..982)
                     /locus_tag="POB92_002675"
     tRNA            complement(907..982)
                     /locus_tag="POB92_002675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(947..949),aa:Thr,seq:tgt)
     gene            complement(990..1062)
                     /locus_tag="POB92_002676"
     tRNA            complement(990..1062)
                     /locus_tag="POB92_002676"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Phe,seq:gaa)
     gene            complement(1081..1156)
                     /locus_tag="POB92_002677"
     tRNA            complement(1081..1156)
                     /locus_tag="POB92_002677"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1121..1123),aa:Asp,seq:gtc)
     gene            complement(1166..1239)
                     /locus_tag="POB92_002678"
     tRNA            complement(1166..1239)
                     /locus_tag="POB92_002678"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1203..1205),aa:Met,seq:cat)
     gene            complement(1254..1346)
                     /locus_tag="POB92_002679"
     tRNA            complement(1254..1346)
                     /locus_tag="POB92_002679"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1308..1310),aa:Ser,seq:tga)
     gene            complement(1385..1460)
                     /locus_tag="POB92_002680"
     tRNA            complement(1385..1460)
                     /locus_tag="POB92_002680"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1425..1427),aa:Asp,seq:gtc)
     gene            complement(1471..1560)
                     /locus_tag="POB92_002681"
     tRNA            complement(1471..1560)
                     /locus_tag="POB92_002681"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1522..1524),aa:Ser,seq:tga)
     gene            complement(1570..1643)
                     /locus_tag="POB92_002682"
     tRNA            complement(1570..1643)
                     /locus_tag="POB92_002682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1607..1609),aa:Ile,seq:cat)
     gene            complement(1672..1745)
                     /locus_tag="POB92_002683"
     tRNA            complement(1672..1745)
                     /locus_tag="POB92_002683"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1709..1711),aa:Met,seq:cat)
     gene            complement(1767..1842)
                     /locus_tag="POB92_002684"
     tRNA            complement(1767..1842)
                     /locus_tag="POB92_002684"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1807..1809),aa:Ala,seq:tgc)
     gene            complement(1860..1933)
                     /locus_tag="POB92_002685"
     tRNA            complement(1860..1933)
                     /locus_tag="POB92_002685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1897..1899),aa:Pro,seq:tgg)
     gene            complement(1943..2016)
                     /locus_tag="POB92_002686"
     tRNA            complement(1943..2016)
                     /locus_tag="POB92_002686"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1980..1982),aa:Arg,seq:acg)
     gene            complement(2035..2123)
                     /locus_tag="POB92_002687"
     tRNA            complement(2035..2123)
                     /locus_tag="POB92_002687"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2087..2089),aa:Leu,seq:taa)
     gene            complement(2134..2208)
                     /locus_tag="POB92_002688"
     tRNA            complement(2134..2208)
                     /locus_tag="POB92_002688"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2174..2176),aa:Gly,seq:gcc)
     gene            complement(2212..2293)
                     /locus_tag="POB92_002689"
     tRNA            complement(2212..2293)
                     /locus_tag="POB92_002689"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2257..2259),aa:Leu,seq:tag)
     gene            complement(2297..2372)
                     /locus_tag="POB92_002690"
     tRNA            complement(2297..2372)
                     /locus_tag="POB92_002690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2337..2339),aa:Lys,seq:ttt)
     gene            complement(2378..2453)
                     /locus_tag="POB92_002691"
     tRNA            complement(2378..2453)
                     /locus_tag="POB92_002691"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2418..2420),aa:Thr,seq:tgt)
     gene            complement(2470..2545)
                     /locus_tag="POB92_002692"
     tRNA            complement(2470..2545)
                     /locus_tag="POB92_002692"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2510..2512),aa:Val,seq:tac)
BASE COUNT          713 a          649 c          595 g          592 t
ORIGIN      
        1 gtaataccaa aaaaagaagt taaacaatgt atagttgctt aacttccaat attgaactca
       61 tcattacaat ttgacataga gtctattaaa gcgtgtgcca tttgagtcca cttttatttg
      121 tattgtatag agagaaataa aaagaaacct tgttttacaa ggtttctaat acgttatgtt
      181 atgtaaataa cagttaatta taccggtggt cggggtcgaa ccgacactcc acaagtggaa
      241 cgggattttg agtcccgcgc gtctgccaat tccgccacac cggcttaatg gtaaacaaaa
      301 aacttccctt tggaagcaat tatggagcgg aagataggat ttacacctat acctcattcc
      361 aggaaggaat gtattctaag agttgaaata ctcccgcatt attattaaat tatggagcgg
      421 aagataggat ttgcacctat acctcgttcc gggaaggaac gtgttctaaa agttgaacta
      481 ctcccgcata aacctggagg cggcaaccgg atttgaaccg gtgataaagg ttttgcagac
      541 ctctgcctta ccacttggct atgccgccaa taactgggct agctggattc gaaccaacga
      601 gtgacggagt caaagtccgt tgccttaccg cttggctata gcccattaat aataagggcg
      661 gctgaagggg atcgaaccct cgaatgtcgg aaccacaatc cgatgtgtta accacttcac
      721 cacagccgcc atggcagggg cagtaggaat cgaacccaca ccaaaggttt tggagacctc
      781 tattctaccg ttgaactatg cccctattaa aaataataaa tggagggggg cagattcgaa
      841 ctgccgaacc cgaaggagcg gatttacagt ccgccgcgtt tagccacttc gctacccctc
      901 cataaatggt gccggccaga ggacttgaac ccccaaccta ctgattacaa gtcagttgct
      961 ctaccaattg agctaggccg gctaagaaat ggttcaggac agagtcgaac tgccgacaca
     1021 tggagcttca atccattgct ctaccaactg agctactgaa ccataataaa aatgtaatga
     1081 tggcggtctc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg cgtgttaacc
     1141 gctacactac gagacctata aaatattgcg ggaggcggat ttgaaccacc gaccttcggg
     1201 ttatgagccc gacgagctac cgaactgctc catcccgcga taataaaaaa taatggcgga
     1261 ggaagaggga ttcgaacccc cgcggcccgt taaggccctg tcggttttca agaccgatcc
     1321 cttcagccgg acttgggtat tcctccatta ttataggtaa atcgctatta attataaaat
     1381 taaatggcgg tctcgacggg aatcgaaccc gcgatctcct gcgtgacagg caggcgtgtt
     1441 aaccgctaca ctacgagacc attagtaaaa cggaggaaga gggattcgaa cccccgcgag
     1501 ccgttaagcc cctgtcggtt ttcaagaccg atcccttcag ccggacttgg gtattcctcc
     1561 aaaattatat ggaccttgca ggactcgaac ctgcgaccga acggttatga gccgttagct
     1621 ctaaccaact gagctaaagg tcctaaatat aattttacaa ctaataaata gtggcggtgg
     1681 aggggatcga acccccgacc tcacgggtat gaaccgtacg ctctagccag ctgagctaca
     1741 ccgccttata tagtttgtaa ataatatggt ggagactagc gggatcgaac cgctgacctc
     1801 ctgcgtgcaa agcaggcgct ctcccagctg agctaagccc ccataataat tacagtatat
     1861 cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa accaagtgct ctaccaagct
     1921 gagctacttc ccgtataatt aacgcgcccg ataggagtcg aacccataac ctcttgatcc
     1981 gtagtcaaac gctctatcca attgagctac gggcgcatat gtttttattg aaaatggtgc
     2041 cgaggaccgg aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg
     2101 tctgccagtt ccgccacccc ggcactataa aaatggagca gaagacggga ttcgaacccg
     2161 cgaccccaac cttggcaagg ttgtattcta ccgctgaact acttctgcat atgcgggtga
     2221 agggagtcga acccccacgc cgtaaggcgc tagatcctaa gtctagtgcg tctgccaatt
     2281 ccgccacacc cgcaaatggt gagccataga ggattcgaac ctctgaccct ctgattaaaa
     2341 gtcagatgct ctaccaactg agctaatggc tcttccatgg tgccggccag aggacttgaa
     2401 cccccaacct actgattaca agtcagttgc tctaccaatt gagctaggcc ggcaatatgt
     2461 aagaataaat ggtggagaat gacgggttcg aaccgccgac cctctgcttg taaggcagat
     2521 gctctcccag ctgagctaat tctccgatt
//
LOCUS       DAMZLE010000226         2543 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMZLE010000226 DAMZLE010000000
VERSION     DAMZLE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.230"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(104..943)
                     /locus_tag="POB92_002693"
     CDS             complement(104..943)
                     /locus_tag="POB92_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6580119.1"
                     /translation="MKKTLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKTPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(1091..1198)
                     /locus_tag="POB92_002694"
     CDS             complement(1091..1198)
                     /locus_tag="POB92_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580120.1"
                     /translation="MYLLKQHMQCAFSNITYGDFQGIELIVENVLNYMV"
     gene            complement(1352..2462)
                     /gene="prfB"
                     /locus_tag="POB92_002695"
     CDS             complement(join(1352..2389,2391..2462))
                     /gene="prfB"
                     /locus_tag="POB92_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI6580121.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
BASE COUNT          740 a          477 c          375 g          951 t
ORIGIN      
        1 tttaaatatc ttctttatat aactctgatt aaatgatacc aaaaaatcct ctcaacctgt
       61 tacttaaaca ggctaagagg atagtcttgt cttgatatat tacttagtgg atgtaattat
      121 attttcctgg atttaaaatt gttcttgaag atttaacatt aaatccagca tagttcattt
      181 cagaaacagt aattgttcca ttagggttta cagattcaac aacaccaaca tgtccatatg
      241 ggccagcagc tgtttggaaa atagcgccaa cttctggtgt tttatctact ttaaatcctg
      301 caacttttgc tgcgtaattc cagttatttg cattgcccca taaacttcct atacttctac
      361 ctaattgtgc acgacgatcg aaagcataat atgtgcagtt tccataagca tataagttcc
      421 ctctgttagc aactgattta ttgtagttat gtgcaacagg tacagttggt actgattttt
      481 gtacttgagc aggtttgtat gctacattaa ctgtcttagc tactgcttgc ttaggtgctt
      541 gcttaactac tactttttta gacgcttgtt gtacaggttg ttttactacc tttttagctt
      601 ggcttgcttt tcttactggt gttttaaccg ctttagtttg tttcacttta ttttgaggca
      661 caagtgaaat cacgtcacca ggaaaaatta aaggtgttac accaggattg tatgaatata
      721 attgattcaa cgttaagtga tgctctaatg caatcttata taatgaatcg ccagcaacta
      781 ctgtataagt tgtcggtgat tgcgtttgtg cttgaacatt tgatacataa ttatgttgaa
      841 caggtgtttt tacttgtgtg ccatgttgtt gtgcatgtgc tgcattattt aaagctaaaa
      901 aagctaacac tgacgaaacc gtcactgtaa gagttttttt catcttgctg tcattccttt
      961 gctgttagta ttttaagtat gcaaatacta tagcacaata cattttgtca aaagctattg
     1021 ttataacgat gtaatcaaat ggttaacaat ataaaaagaa tacaaccttt tatcatagtg
     1081 taaaatgtat tcataccatg taattgagaa cgttttcaac gattaattca ataccttgaa
     1141 aatcgccata ggtaatatta ctaaatgcac actgcatatg ttgttttaac aaatacaact
     1201 ttcaaaaaat atattctaac tctatctacc gaattgtact taaatattca taaacaaatc
     1261 atattccaaa atctaattta caatttattt agctaccttt aaaaaaacca aaaaccgacg
     1321 cccttttaga gcctcggttt taaaatatat attaatcgtg cgacattgtc tgtcttaaat
     1381 atgattcgat aaatggtcca atgtctccat ccatcactgc atcaacctta cctgtttctt
     1441 cgttcgtacg atgatctttc accattgagt atggatggaa aacatatgat ctaatttggc
     1501 ttccccagcc gatttctttt tgttcgccac gaatttcagc catttcacgt gcctgctctt
     1561 ccaattttaa ttgatataat ttagacttta acattttcat agctgcttca cggtttttaa
     1621 tttgagaacg ttcattttgg ttattaacaa ctatacctga ggggtggtgg gtaattcgta
     1681 ttgccgattc agttttgtta atatgctgac cacctgcacc agaagctctg aatgtatcaa
     1741 ctgtaatatc atccggattg atttcaatct ctatttcatc attattaaaa tctggaataa
     1801 cgtcgcatga tgcaaatgat gtatgacgac gtcctgatga atcaaatgga gaaattcgta
     1861 ctagtcggtg tacacctttt tcagctttta aataaccata agcattatgc cctttgatga
     1921 gcaatgttac acttttaatc cctgcttcat ccccaggtag ataatcaaca gtttcaactt
     1981 taaagccttt cttctcacaa taacgttgat acattctaaa tagcatatta gcccaatctt
     2041 gagactccgt gccacctgca ccaggatgta actctagaat tgcgttattg gcatcgtgag
     2101 gcccatctaa taataattgc aattcgtatt catccacttt agccttaaaa ttaatgacct
     2161 cttgctctaa gtcttctttc atttcttcat caaattcttc ttgtaataaa tcccaagtag
     2221 catccatgtc atctacttct gcttgtagtg ttttataacc attaactatt gcttttaacg
     2281 cattattttt atctataata tcttgcgctt tcgtttggtt atcccaaaaa ttaggttctg
     2341 ccatcatttc ttcatattct tgaatattag tttctttgtt ctctaagtca aagagacccc
     2401 ctaatttgtg ttaaatcttg attatactta tctatatttc gtttgatttc tgataattcc
     2461 atagcattcg ctcctattta tatttcaatt caagtcattg atttgtatct tttataatgc
     2521 taaattttaa cataattttg tta
//
LOCUS       DAMZLE010000227         2527 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMZLE010000227 DAMZLE010000000
VERSION     DAMZLE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.231"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            74..1039
                     /locus_tag="POB92_002696"
     CDS             74..1039
                     /locus_tag="POB92_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6580122.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            1374..2450
                     /locus_tag="POB92_002697"
     CDS             1374..2450
                     /locus_tag="POB92_002697"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDI6580123.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT          897 a          333 c          503 g          794 t
ORIGIN      
        1 tattatgaaa atgaatatta tgtccgcttg tgatggacta tagctatata gaagagacaa
       61 aggagatatt tctatgaaaa aaattatgat tactggtgca ttaggacaaa ttggtacaga
      121 attagttgtt aagtgcagag aaatttatgg gacagataat gttcttgcta cagatattag
      181 ggaacctgaa gcagactcac ctgtacaaaa tggaccattt gaaatcttag acgtaacaga
      241 tcgtgaccgt atgtttgagt tagttaggga ctttgaagcg gatagtctaa tgcatatggc
      301 agcattatta tcagcaactg ctgagaaaaa tccaattcta gcttgggatt taaatatggg
      361 tggattaatg aatgcattag aagctgcaag aacttataat ttgcactttt tcacaccaag
      421 ttcaattggt gcatttggag actcaactcc taaagttaat acgccacaag taacgattca
      481 gcaacctacg acaatgtatg gtgtaaataa agtagctgga gaattattgt gtcaatacta
      541 tttcaaacgt tttggtgtag atacaagaag tgttagattc ccaggtttaa tctcgcatgt
      601 taaagagcca ggtggcggta ctacagacta tgctgttgaa atatacttca aagcagtaag
      661 agagggtcat tatacaagct tcatagataa aggcacgtat atggatatga tgtatatgga
      721 tgatgcaatt gaagcaatta ttaaacttat ggaagcagac gacgctaaat tagaaactag
      781 aaatggttat aatttgagcg caatgagttt tgatccagag atggtaaaag aagcaattca
      841 agaatactat cccaatttta cattagatta cgatgttgat cctattagac aaggtatcgc
      901 taatagttgg ccggattcta ttgatacaag ctgttcacgt ggcgaatggg gatttgatcc
      961 taaatatgat ttagcgagca tgactaaatt aatgttagaa gctattgaac aaaaagatac
     1021 tgttaaaaat aataactaat catttccatt cactttaata cacggaatga tattttaaat
     1081 tactctttat tttaataaac tagtgcatga attctaatat tattcattat acatattgaa
     1141 ttcgcgagct tagttcattt taaagtaaag agttttttgt atgtaaagta tattagtaaa
     1201 acacagatta cttgtctctg tgaaattaat acgtttatac taaaagatat agtctttcta
     1261 gaaatctgtt aattaatttt gaatttttag aaaatttgtt gaacagcaaa atatggattg
     1321 ttataattta agttaaacaa attcttatac aatattatta ggaggcaatc accatgtcac
     1381 aagcagttaa agttgaacga cgagaaacat taaaacaaaa accaaataca tctcaactag
     1441 gttttggtaa atattttact gattatatgt tgagttatga ttatgatgca gataaaggat
     1501 ggcatgattt gaagatagta ccttatggtc ctattgaaat ttcacctgct gcacaaggtg
     1561 ttcattatgg tcaatcggta ttcgaaggat taaaagcata taaaagagat ggggaagttg
     1621 cacttttccg tcctgaagaa aattttaagc gtcttaataa ctcgttagca cgattagaaa
     1681 tgcctcaagt agacgaagca gaattgttag aggggctaaa acaattagtt gatatagaaa
     1741 gagattggat tcctgaaggg gaaggtcaat cattatatat tcgtccattt gtttttgcaa
     1801 cagaaggggc acttggcgtt ggtgcatcac atcagtataa attattaatt attttatctc
     1861 cttcaggtgc atattatggt ggtgagactt taaaaccaac taaaatctat gtagaagatg
     1921 aatatgtgcg tgctgttcgt ggcggtgtag gctttgcaaa agttgcaggt aactatgcgg
     1981 caagtttatt agcacaaaca aatgcaaata aattaggtta tgaccaagta ttatggcttg
     2041 atggtgttga acagaaatat atcgaagaag ttggtagcat gaatattttc ttcgttgaaa
     2101 atggaaaagt aattacacca gagttgaatg gcagtatttt acctggtatt acacgtaaat
     2161 ctattatcga attagctaaa aacttaggat atgaagtcga agagcgccgc gtttcaatcg
     2221 atgaattatt cgaatcatat gataaaggtg agttaacaga agtatttggt agtggtactg
     2281 cagcagttat ttcacctgtg ggtacattga gatacgaaga tcgtgaaatc gttattaata
     2341 ataatgagac tggtgaaatt actcaaaaat tatacgacgt ctatactggt attcaaaatg
     2401 gtactttaga agataaaaat ggttggagag tcgttgtacc aaaatattaa taaaaattga
     2461 atataatcat gaaaacaata tgtaaaagtt aatcatgact aaaatccctt tcaatgaagc
     2521 gaagcat
//
LOCUS       DAMZLE010000228         2501 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMZLE010000228 DAMZLE010000000
VERSION     DAMZLE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.233"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1920
                     /gene="rsp"
                     /locus_tag="POB92_002698"
     CDS             <1..1920
                     /gene="rsp"
                     /locus_tag="POB92_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDI6580124.1"
                     /translation="NIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIADLIS
                     TPTRSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNIDAHL
                     SLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTVSELA
                     GFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTIDLTAQ
                     LATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPVVFID
                     DIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQDYKT
                     SKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQVYDVM
                     QRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVYNNLS
                     VSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSPLPLM
                     HLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNELPQD
                     SLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGRTFEL
                     KLNHDEVKYICFKPS"
     gene            1991..2413
                     /locus_tag="POB92_002699"
     CDS             1991..2413
                     /locus_tag="POB92_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDI6580125.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKIDL"
BASE COUNT          936 a          380 c          342 g          843 t
ORIGIN      
        1 aatataatcg aattaatgat acccgttttt tatttttatc aacaagatga tgatttcttt
       61 aatggttatt tagatcgtca tttattacaa tcaagtaatt atattaagtc attaattgcc
      121 gatttaatca gtacaccaac aagatcttct ttaatgggga aaaatatagg tcaaagtatt
      181 atagacactt tacttaaaga agcttttata agaatagatc atgaatattt accaaatatt
      241 gctttaagca atccggtgtt catagactgt gtaaactata ttcatgacaa tattgatgcg
      301 cacttgtctt taaaagatat agcgatgcat tgcaacatct ctgaatcata ttgttctaat
      361 ctttttgttc gatatttgag tatgaatttt aaagattatt ttacaagtat taaacttgtt
      421 aacgccatca atttattact ttctacaaaa cattctatta caactgtttc tgaattggcc
      481 ggttttaaca gtcatacaaa ttttgcaaat caatttaaga attacttgca ttttagtcct
      541 aaacagttcc gttcactcgt ttccaagatt actgaaccac cacaaataca cttccaacaa
      601 gataacgtat cacaattcac tgagttaatt tcaacaattg atttaacagc tcaactggct
      661 acaaatacaa cagatattca tatagatgat tttaatccga aagatcgaag tcagcgtgcc
      721 aaagtatttg ttcgatttag taatttcaat gaactatttc aatttatttt caatgaatat
      781 tacgatatta actttgagca cttaccaaaa cctgtcgttt tcattgacga tattcacgat
      841 attgagatta gccaaacgaa ttacaacctg ttaaatagat gttttgaaaa gcttttcgaa
      901 aaaaatatag gtttagcaat cgctattaaa tcaacacaac aattcgaaac gatgaagcaa
      961 ttaatactca catttttaca aggcaatcaa gactataaga caagcaagaa attagttaaa
     1021 tttatgctcg tattttgctc taattctatg acagcagaag aaattcattt atgtcatctt
     1081 aaaattaaaa acaaaaataa agaaattaaa tatagtgtga cagtcgatgg ctttttagaa
     1141 acttattcaa ccgtggaaca agtttatgat gtcatgcagc gtcttaaatt ccattattac
     1201 tttattgata ttgaaaattc aaaaactgca acacatctta tcactaaaaa ccaacactat
     1261 catcaaactg acactcattt tgaacagtat aagaaattta ttttagattc aggtatatca
     1321 tcaacacaat ttgtatataa taacctgtct gtaagcggat ttaaatatac taatgatggt
     1381 aagaatccaa ttcaattatc tgacatagtg tatcacttaa ttgcattatt acgatatggc
     1441 ggtggcatta gctatcaatt attagatgac cattcaaatt atatttcctt gtacaacaaa
     1501 tatggtagtc ccctaccatt aatgcattta tataaaatgt ttagaccttt tgttaatgaa
     1561 gatattgaaa ttacaaataa ttatgtattg agtcgtaaag ataataacta ccatttctta
     1621 ttattcaata aaattaatga tcgatatatg tcagacgtaa aacaagattt cattttccat
     1681 aatgaattac ctcaagactc tttgatgatt attaaaacat tgaatcatga acatggttca
     1741 attcaacatt tgcttccaat aagcgatcaa cttgtttata tagaaaaaga aattttagat
     1801 gaattagaca aaacgaatta ccctaaaacg gagcttgcag ttcaagaaga aactggtcgt
     1861 acatttgaac tcaagttaaa tcacgacgag gttaaatata tttgctttaa accaagctaa
     1921 atactaacag tcctcttgtg tttagtttct tacgttaaag gctatttata tcataaggag
     1981 atgatatgta atgagtaact cacaagcaat tcaagcaatt gaaaacgtgt tagcaacgtc
     2041 aaaagttggt gtattatcaa ctgcatataa taataaacct aatagtagat atatggtctt
     2101 ttataatgat ggtcttactt tatatactaa aacgaatatc cattctgcta aggtcaaaga
     2161 aattaaagat aatccagcag catatgtttt gttaggctac aatgatacga caaatcgcag
     2221 tttcgttgaa atggaagcca cgatagaagt agttactgat caaaaagtaa tcgattggtt
     2281 atgggagact caagataaaa gctttttcag ttcaaaagaa gatccagaac tttgtgtttt
     2341 aaaggtgaca ccacaatctg ttaaattgat gaatgacaaa tcattagata cacctatcaa
     2401 aatcgattta taacacaaag tatatatagg aaataacttt tatgaattct agatataaca
     2461 atgttaaata cttaaaataa ctcgctataa ttaaagtgtt t
//
LOCUS       DAMZLE010000229         2473 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMZLE010000229 DAMZLE010000000
VERSION     DAMZLE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.234"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(397..1131)
                     /gene="erm(C)"
                     /locus_tag="POB92_002700"
     CDS             complement(397..1131)
                     /gene="erm(C)"
                     /locus_tag="POB92_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="HDI6580126.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALFLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
     gene            complement(1192..1251)
                     /gene="ermCL"
                     /locus_tag="POB92_002701"
     CDS             complement(1192..1251)
                     /gene="ermCL"
                     /locus_tag="POB92_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDI6580127.1"
                     /translation="MGIFSIFVISTVHHQPNKK"
     gene            complement(1946..2422)
                     /locus_tag="POB92_002702"
     CDS             complement(1946..2422)
                     /locus_tag="POB92_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDI6580128.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
                     QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
                     GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
BASE COUNT          751 a          388 c          375 g          959 t
ORIGIN      
        1 aaaaaaaaaa gaacatttgt taaaagaaat tagaacaaaa tgagtgaaaa attagaacaa
       61 acaaattcct tataaacctt atcatctcaa cctatattaa gattttacct agttgaatct
      121 tcttttctat ataaagcgtc ggagcatatc agggggttat ctaacgtaaa tgctaccctt
      181 cggctcgctt tcgctcggca ttgacgtcag atactgcacc ccctgaaccc ccatgctcca
      241 acagcaaaaa ggaaactttt tgctgctttt ccgacgctta ttcgcttcgc tcatatttat
      301 atagaaaaga agtgaatgcg caaaagacat aatcgattca caaaaaatag gtacacgaaa
      361 aacaagttaa gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct
      421 attgaaaaga gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg
      481 ttttaaggaa ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca
      541 tttcataacg aaataattat acttctgttt atctttgtgt gatattcttg atttttttct
      601 atttaatctg ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac
      661 catacttaat atagaaatat caacttctgc cattaaaaat aatgccaatg agcgttttgt
      721 atttaataat cttttagcaa acccgtattc cacgattaaa taaatctcat cagctatact
      781 atcaaaaaca attttgcgta ttatatccgt acttatgtta taaggtatat taccaaatat
      841 tttataggat tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa
      901 attatcgtga tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat
      961 ggcagttacg aaattacacc tctgtactaa ttcaagggta aaatgccctt ttcctgagcc
     1021 gatttcaaag atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt
     1081 atgttttgaa gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct
     1141 ctttaatttg gttataatga attttgctta ttaacgattc attataacca cttatttttt
     1201 gtttggttga tgatgaactg tgctgattac aaaaatacta aaaatgccca tattttttcc
     1261 tccttataaa attagtataa ttatagcacg agctctgata aatatgaaca tgatgagtga
     1321 tcgttaaatt tatactgcaa tctgatgcga ttattgaata aaagatatga gagatttatc
     1381 tagtttcttt ttttacaaga aaaaagaaag ttcttaaagg ttttatactt ttggtcgtag
     1441 agcacacggt ttaacgactt aattacgaag taaataagtc tagtgtgtta gactttaatg
     1501 tttttttaag gcattagtgc atttaagcgt cagagcatgg ctttatgccg agaaaactat
     1561 tggttgggat ggcgtgtgtg ttagccaaag ctttggcgag ttggttgggg gtttcatggg
     1621 attaatccca tgaaagtacc aactcaacaa cacactaacg cctgttggtt ccaaccaata
     1681 ggaaattgga ataagcaatt agtataatga gagtataatg ttggtataac gttagtataa
     1741 tgatgctttt tttcattata ttttttatgt actttaaacc tgcacgctta tgtgaattag
     1801 aaaaagctta atcgcatttc atagattgac ctcccaataa caccacgtag ttattgggag
     1861 gtcaatctat ttcatttgcg ttaagctttt gatctacatt aagcgtgcag gttttttgtc
     1921 tatttgtaga cgattttatg ttatactagt ctttgaaaga ataataatca gataatgcat
     1981 tttcttgttt ttcatttgcc tcttgctcaa agttcccaaa ttcgagtaag aggtattttt
     2041 gtttttggtc gtcgcctctc attagtagtt cagggtttaa cattaatact ccagtttttc
     2101 tttttataat atttccttct tctaagattt taagtgttgt tattactgtt tgtagacttg
     2161 ttcctgtagc ttttgctatt tctcttgttg tagctatcat tgtattgtta cttaagtgga
     2221 cattatctag gatatagtta acgattttaa gtttttttcc gccaatcata tctaacatac
     2281 ttattaattg cactatatat gcctttacga agttaccaga cgtttgttta cggtataact
     2341 tgtctacctc tatgacttct ccactttctt cgtctatgag cctctgagag cctttataga
     2401 ctgttccata tctttctttc atctttttct cactccttat tttaaactat tctaactata
     2461 tcataactgt tct
//
LOCUS       DAMZLE010000230         2429 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMZLE010000230 DAMZLE010000000
VERSION     DAMZLE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.235"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            122..>2429
                     /gene="sdrC"
                     /locus_tag="POB92_002703"
     CDS             122..>2429
                     /gene="sdrC"
                     /locus_tag="POB92_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HDI6580129.1"
                     /translation="MNNKKTVTNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTEKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATASQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKDVSATPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHLNKDTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVSLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTNQFNITYSNDNKTATVDLMNGQTSSNKQYIIQQ
                     VAYPDNTSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANAGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSD"
BASE COUNT          996 a          373 c          419 g          641 t
ORIGIN      
        1 aaagaaaaat aattaaagta ttgcacttta ttgaaattta tattacgata gcaatgcaga
       61 aatttatata tgcaaaatat tatatttatc aaattttgat attttaaagg agtattatta
      121 aatgaataat aaaaagacag taacaaatag aaaaggcatg ataccaaatc gattaaacaa
      181 attttcgata agaaagtatt ctgtaggtac tgcttcaatt ttagtaggga caacattgat
      241 ttttgggtta agtggtcatg aagctaaagc ggcagaacat acgaatggag aattaaatca
      301 atcaaaaaat gaaacgacag ccccaagtga gaataaaaca actgaaaaag ttgatagtcg
      361 tcaactaaaa gacaatacgc aaactgcaac tgcagatcag cctaaagtga caatgagtga
      421 tagtgcaaca gttaaagaaa ctagtagtaa catgcaatca ccacaaaacg ctacagctag
      481 tcaatctact acacaaacta gcaatgtaac aacaaatgat aaatcatcaa ctacatatag
      541 taatgaaact gataaaagta atttaacaca ggctaaagat gtttcagcta ctcctaaaac
      601 aacgactatt aaaccaagaa cattaaaccg tatggctgtg aacacagtag cagcaccaca
      661 acaaggtaca aatgttaatg ataaagttca tttttcaaat attgatattg ctattgataa
      721 aggacactta aataaagata ctggcaaaac agaattttgg gcaacttcaa gtgatgtttt
      781 aaaattaaaa gcgaattaca caatcgatga ttctgttaaa gagggcgata catttacttt
      841 taaatatggt caatatttcc gtccaggatc agtaagatta ccttcacaaa ctcaaaattt
      901 atataatgcc caaggtaata ttattgcaaa aggtatttat gacagtacaa caaacacaac
      961 aacgtatact tttacgaatt atgtagatca atatacaaat gttagcggta gctttgaaca
     1021 agtcgcattt gcgaaacgtg aaaatgcaac aactgataaa actgcttata aaatggaagt
     1081 aagtttaggt aatgatacat atagcgaaga aatcattgtc gattatggta ataaaaaagc
     1141 acaaccgctt atttcaagta caaactatat caacaatgaa gatttatcgc gtaatatgac
     1201 tgcatatgta aatcaaccta aaaatacata tactaaacaa acgtttgtta ctaatttaac
     1261 tggatataaa tttaatccaa atgcaaaaaa cttcaaaatt tacgaagtga cagatcaaaa
     1321 tcaatttgtc gacagtttca ccccagatac ttcaaaactt aaagatgtta ctaatcaatt
     1381 taatattact tacagcaatg ataataagac tgcaacagtt gatttaatga atggtcaaac
     1441 aagtagtaat aaacaataca tcattcaaca agttgcgtat ccagataata catcaacaga
     1501 taatggaaaa attgattata ctttagacac tgacaaaact aaatatagtt ggtcaaatag
     1561 ttattcaaat gtgaatggct catcaactgc taatggcgac caaaagaaat ataatctagg
     1621 tgactatgta tgggaagata caaataaaga tggtaaacaa gatgccaatg aaaaagggat
     1681 taaaggtgtt tatgtcattc ttaaagatag taacggtaaa gaattagatc gtacgacaac
     1741 agatgaaaat ggtaaatatc agttcactgg tttaagcaat ggaacttata gtgtagagtt
     1801 ttcaacacca gccggttata caccgacaac tgcaaatgca ggtacagatg atgctgtaga
     1861 ttctgatgga ctaactacaa caggtgtcat taaagacgct gacaatatga cattagatag
     1921 tggattctac aaaacgccaa aatatagttt aggtgattat gtttggtacg acagtaataa
     1981 agatggtaaa caagattcga ctgaaaaagg aattaaaggt gttaaagtta ctttgcaaaa
     2041 cgaaaaaggc gaagtaattg gtacaactga aacagatgaa aatggtaaat accgctttga
     2101 taatttagat agtggtaaat acaaagttat ctttgaaaag cctgctggtt taactcaaac
     2161 aggtacaaat acaactgaag atgataaaga tgccgatggt ggcgaagttg atgtaacaat
     2221 tacggatcat gatgatttca cacttgataa tggctactac gaagaagaaa catcagatag
     2281 tgactcagat tcagacagcg actcagactc agattcagac agcgactcag actcagatag
     2341 cgactcagat tcggacagcg actcagattc agatagcgac tcagattcgg acagcgattc
     2401 agactcagac agcgactcag actcagaca
//
LOCUS       DAMZLE010000231         2414 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMZLE010000231 DAMZLE010000000
VERSION     DAMZLE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.236"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(117..284)
                     /locus_tag="POB92_002704"
     CDS             complement(117..284)
                     /locus_tag="POB92_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580130.1"
                     /translation="MKRIVILENVIVDDPIRLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(412..915)
                     /locus_tag="POB92_002705"
     CDS             complement(412..915)
                     /locus_tag="POB92_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6580131.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(908..1495)
                     /locus_tag="POB92_002706"
     CDS             complement(908..1495)
                     /locus_tag="POB92_002706"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDI6580132.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1507..2307)
                     /gene="racE"
                     /locus_tag="POB92_002707"
     CDS             complement(1507..2307)
                     /gene="racE"
                     /locus_tag="POB92_002707"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI6580133.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
BASE COUNT          753 a          457 c          311 g          893 t
ORIGIN      
        1 tatttattaa ctaaacatta atatttgtgt cactacattt ctgaatttca agaattttgc
       61 tactccatat tttaaccatc aaattattga tataatttat gactttagaa atccttttaa
      121 atatgtatac gattcatgat tttaactaaa atttcaaatt tcctgacttc ttggaatctt
      181 aattttatac tttttaataa tacactttta accaaataat caacatgatt caaccttata
      241 gggtcatcaa caatcacatt ctccaaaatt acaattcttt tcataacata aatgtgttat
      301 tcattccaat tatcttataa aattgtcaga aatactttta aacaaacatt catgacacga
      361 cctcctagtt aaatcagtct ataaactaaa aaaagccata gctacttcta tttaaaaagt
      421 agtatgactg attgtttcgt gatgtcgatt tttgaaattg atggtgccat gtaaagtttg
      481 atcatcaata ataacttcag catatgtttc ttccattgaa cttctagatt gagatatact
      541 tccaggatta ataacatgaa caccattaat atactcatat tttgccacat gtgtatgtcc
      601 ataaaatgca aacaaacaac ctaattcaag tcctttttca gctaataaat ctcttgttcg
      661 attgacttga tataaatgtc catgagtata aaatgctttc acgtcattat attttgcgac
      721 cgcttcattt tcaaattctg gataaaaatc acaattgccc tttactctat taaataagct
      781 aagttccgta tcatcatacg cgaattctga atctcctaaa tgtaaatata catctgcatc
      841 tgggtgcatt tcataaattt gatataaaac gcctgattca gtatggttat cactcacaat
      901 aatccattta gacattttta tcaccttcaa gaaaagcttg aagtaaatta atcgcatttc
      961 gtctatgact aatttgccct ttttgttctt ttgaaagttg agccatggtt ttatctaatt
     1021 tcggtacata aaatatcgga tcatatccga aaccattttc gccatatttt ccatctgcaa
     1081 tttcacctga aacagtacct ttaaatactt ttgtttccat atcagggcca ctcatactta
     1141 tgacacaaac aaattgcgca cgacgatcag ttgtattacc aagcttattt aataattttt
     1201 caatatttgc ttcatcgctt ttattttcac cagcataacg tgcagagtat atacctggct
     1261 caccatttaa tgcaaaaact tctagtccac tgtcatcagc tatgactgtt ttattcaatg
     1321 cttttgcagc agcttctgat tttaatatag cattttcttc aaatgttgat cctgtttctt
     1381 ccacatcaaa atctggtatt agttctgaaa tacctattac gtggtaatct ggaaatatta
     1441 ctttaaagtc atttattttc ccttgattat tcgatgcaat aacaatctct ttcattaaaa
     1501 atcctcctag tcattcactg atatacgttc cacattgaca gataaattta accactcttt
     1561 gataatgtta gtaatgtgag taggatcacc tgttgcaaaa aatcgatgat ctggatgttc
     1621 agtataactt gcatgttcat tactaaatgt tagcaatgca ctaacttcac gagccgtttc
     1681 taatccagac gaaatcactg tctttttacc accaaaataa tcatagatag gtttatagag
     1741 caatggatag tgggtacatc ctaaaatgac agtatcagac tcactattac gccaacgttt
     1801 cagtgtttga tgaatgacaa tgcttgtaat tgttggatca ctatatctca tttgttctac
     1861 aagtggcaca aaacctggac aggcaacgcc atgtacctct acatgtggat tgatacgttt
     1921 aatatgcgta cgatatgctt cagatttaat tgtgccttcc gttcctagta ctaatacatt
     1981 ttgatttcta gtagtcatta ttgctgttct agcacctggt tcaattacgc caatcactgg
     2041 gattgataag gtcttttgta aatattctaa agctacagca gttgctgtat tacaagcaat
     2101 cacgagcatt tttatatcaa attccattaa tttacgagcg atttcaactg tatattgttt
     2161 tacttgttct cctggtcttg gcccatatgg acatcgtcca atatcaccta agtaataaat
     2221 cgtctcattt ggcaactgac gcataatttc tttagctact gtcaaacctc cgacaccaga
     2281 gtctattaca cctattggtt tattcatatt cggtcatcct tacttttaat ttaattttta
     2341 gtttatcata actaagcatt ggattttagt attatgcact gtgtttacca tttttgtcat
     2401 tataatattt attt
//
LOCUS       DAMZLE010000232         2344 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMZLE010000232 DAMZLE010000000
VERSION     DAMZLE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.238"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..656
                     /gene="seh"
                     /locus_tag="POB92_002708"
     CDS             <1..656
                     /gene="seh"
                     /locus_tag="POB92_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal enterotoxin type H"
                     /protein_id="HDI6580134.1"
                     /translation="EDLHDKSELTDLALANAYGQYNHPFIKENIKSDEISGEKDLIFR
                     NQGDSGNDLRVKFATADLAQKFKNKNVDIYGASFYYKCEKISENISECLYGGTTLNSE
                     KLAQERVIGANVWVDGIQKETELIRTNKKNVTLQELDIKIRKILSDKYKIYYKDSEIS
                     KGLIEFDMKTPRDYSFDIYDLKGENDYEIDKIYEDNKTLKSDDISHIDVNLYTKKKV"
     gene            674..1411
                     /locus_tag="POB92_002709"
                     /pseudo
     CDS             674..1411
                     /locus_tag="POB92_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492129.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
     gene            1552..2025
                     /locus_tag="POB92_002710"
     CDS             1552..2025
                     /locus_tag="POB92_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6580135.1"
                     /translation="MEYKSYNKMSNNILSILLSDKNFDNKMVVSTGSYILSELPCIVA
                     YKNNDIAGLLTYKVYDEYIEIISLDSFVENKGIGSHLLDYAEIIASDMSKRSISVITT
                     NENIKALYFYQKNKYRITDVIFDAVTEARKIKPSIPTIDKNGIEIRDEIVLTKYL"
     gene            complement(2130..2344)
                     /locus_tag="POB92_002711"
                     /pseudo
     CDS             complement(2130..2344)
                     /locus_tag="POB92_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          925 a          279 c          381 g          759 t
ORIGIN      
        1 cagaagattt acacgataaa agtgagttaa cagatttagc tttagctaat gcatatggtc
       61 aatataatca cccattcatt aaagaaaata ttaagagtga tgaaataagt ggagaaaaag
      121 atttaatatt tagaaatcaa ggtgatagtg gcaatgattt gagagtaaag tttgcaactg
      181 ctgatttagc tcagaagttt aaaaataaaa atgtagatat atatggggca tctttttatt
      241 ataagtgtga aaaaataagt gaaaatattt ctgaatgtct atatggaggt acaacactaa
      301 atagtgaaaa attggcacag gaaagggtga ttggtgctaa tgtttgggta gatggtattc
      361 aaaaagaaac agaattaata cgaacaaata agaaaaatgt gacattgcaa gaattagata
      421 taaagatcag aaaaatattg tccgataaat ataaaattta ttataaagac agcgaaataa
      481 gtaaaggtct aattgaattt gatatgaaaa ctcctagaga ttactcattc gacatttatg
      541 atttaaaagg agaaaatgac tatgagatag ataaaattta tgaagacaat aaaactttaa
      601 aatctgatga tataagtcat attgatgtaa atctatatac taagaaaaaa gtataatcaa
      661 tgaaaatttc tagatcaatg ttagtgttaa ttgtcatgtc ttttttccgt tatggaattc
      721 atttaagcat ttttctcaaa caagtacata tactccaaaa gcagaaactc taaagaacaa
      781 gtccgactta gatttaaccg cgttatataa tattaaacct gtatattcag aaagtgaatt
      841 tcttaaattt taaagataaa attaaaacca agcaactatt atataattat ttaatgttat
      901 aattaaataa ttgaataaag atagtctaaa aaaagagctt aatagttcgg aaattgcaaa
      961 cacattcaaa ggaaaaatgt agatatattc ggagtacact ttggagcaaa ttgtgttgga
     1021 gaagcaggtg agaatagcgc ttgtttatat ggtggagtca catttcacga aaaaatgaaa
     1081 aacaacaaaa aagtcaatag gcataaatgt atttaaaaat ggatttcaac aaaaagggtt
     1141 tgtcattact actgctaaaa tcaaacccac agctcaagag ctagatctta aaacgagaaa
     1201 caatatacaa aatcaatata aaatgtatga ttcggaaact ggagatatac aaaaaggata
     1261 tataaaattt cattctacta aatcaagttt ctattatgac ttatttgatt ttaaggtgaa
     1321 aaaacgtgtg gatttcttaa agttctataa tgacaatgaa cttataagta ccaaaaaatt
     1381 acatatagat atctatttgt ttaacaagta aataattgga aacgtttatt gataccttac
     1441 agccatatgg gcagacaaaa tgtcagttta tcttttcatc agttgtgtaa attactttgc
     1501 aaaaacttag taagtgtggg aaagattatt aacagagaga agtgacttat tttggaatat
     1561 aaatcttaca acaaaatgag taataatatt ttatctatct tgttgagtga taaaaatttc
     1621 gataataaaa tggtagtctc aacaggtagt tacattttgt ctgagttgcc atgtattgtt
     1681 gcctataaaa ataatgatat cgcggggcta ctaacgtata aagtttatga tgagtatatt
     1741 gaaatcattt ctttagatag ttttgtagag aacaagggta ttggtagtca tttgcttgat
     1801 tatgctgaaa taattgcctc agatatgagt aaaagaagta tcagtgttat tactacaaat
     1861 gaaaatatta aggcacttta tttctatcaa aaaaataaat atagaatcac agacgttatt
     1921 tttgatgctg taacagaagc taggaaaata aagccgtcga ttcctacaat tgataaaaat
     1981 ggcattgaaa ttagagatga aattgttttg acaaaatatt tgtaatatat aaatatacgt
     2041 tgttttaaat caccctagtt cataccaaaa agactttaac acaaacaaaa aaggaggaaa
     2101 cttaaattcc tccttgaccc tcatttcatc tataattcta ttttaacgtc ttcgtccatt
     2161 tgggcttcaa attcatctag tagtgctcgt gcttctgcaa ttgattgtgt gttcatcaat
     2221 gccatatttt tctgcaagtt caagtcctgt ttttctatcg ggaatatcac cgttaattgt
     2281 taacaatgta tttggtgcaa tttcgtcacg taaattttta atagcttcga ttaattccca
     2341 atgt
//
LOCUS       DAMZLE010000233         2282 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMZLE010000233 DAMZLE010000000
VERSION     DAMZLE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.239"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..466
                     /locus_tag="POB92_002712"
     CDS             <1..466
                     /locus_tag="POB92_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDI6580136.1"
                     /translation="LLCVENSYFKNKANYITQEEWVNLWQKALQVNYRPVANIKAIKP
                     NQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLKQ
                     KHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
     gene            complement(1093..1416)
                     /gene="qacC"
                     /locus_tag="POB92_002713"
     CDS             complement(1093..1416)
                     /gene="qacC"
                     /locus_tag="POB92_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /protein_id="HDI6580137.1"
                     /translation="MPYIYLIIAISTEVIGSAFLKSSEGFSKFIPSLGTIISFGICFY
                     FLSKTMQHLPLNITYATWAGLGLVLTTVVSIIIFKEQINLITIVSIVLIIVGVVSLNI
                     FGTSH"
     gene            1738..>2282
                     /locus_tag="POB92_002714"
     CDS             1738..>2282
                     /locus_tag="POB92_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDI6580138.1"
                     /translation="MQYNTTRCIDENQDNETLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEEVVKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFL
                     RSTEVTVNKNDGSYNQHMHVL"
BASE COUNT          829 a          288 c          409 g          756 t
ORIGIN      
        1 tttattatgt gttgaaaata gttattttaa gaataaagct aattatataa ctcaagaaga
       61 atgggttaat ttatggcaaa aagcattaca agtaaattat cgacccgtag caaatattaa
      121 agcgatcaaa ccaaatcaaa aaggcgataa agatattcaa gcagctatca aagaaacctc
      181 taaatattcg gttaagtcat ctgatttttt aactgatgat gatgaaagaa atcaagaaat
      241 cgtgaatgat ttagaaaaag gtttatatcg aaaacgtatg ttgagttatg gtggtttgct
      301 taaacaaaaa cataagattt taaatttaga tgatgccgaa gatggcaatt tgattaatac
      361 aagtgacgaa gataaaacaa cagacgaaga agaaaaagca cattcaatta cggcaatttg
      421 gaattttgaa aaacaaaatt attatttaaa agatttgaaa cgttagctaa aaagctagcg
      481 tttttttatg tctttttgcc cctgcgggaa ctatagcgtt caaccggcat ggttgaccag
      541 ttttcatgtt aacttttaag tttgcatgta aatgggcagt gtttattttt ttctgcccag
      601 tttgctaaat ttttaatttg catataactg ggcagtgtct aaaaaattag tcactggttt
      661 tgctcaaatt tttgtttgtg aaattaagat atatttattt ggctcatatt tgcgttttaa
      721 aagcgtatat agacttttag gtataaaact atatgattta cccctgaatc tttaaaatgc
      781 ccccttaaaa ttcaaataaa ggcatataaa aataaaaagc caacccagat aaaatggatt
      841 ggctttttat ttgcttttgc gaagtagatt ataaaaaact acttttaata agcaccctaa
      901 atgtgatgtc tatattataa caagaacacg catttatgcc gagaaaattt attgtgcgtt
      961 gagaagaacc cttaactaaa cttgcagacg aatgtcggca tagcgtgagc tattaagccg
     1021 accattcgac aagttttggg attgttaagg gttccgaggc tcaacgtcaa taaagcaatt
     1081 ggaataaagc aattaatgcg atgttccgaa aatgtttaac gaaactacgc cgactatgat
     1141 taaaactata gatactatag ttattagatt tatttgttct ttgaaaataa ttattgagac
     1201 tacggttgtt aagactaaac ctagtcccgc ccaagttgca taagttatat ttagtggtag
     1261 gtgttgcatt gttttactta aaaaatagaa acaaattcca aatgaaatta ttgttcctaa
     1321 ggatggtata aattttgaaa agccttctga agatttaaga aatgcacttc caataacttc
     1381 agtacttatg gctattatta aataaatata aggcattttt aactcctttt aattttcgta
     1441 ttttatttaa ttttattcta agtatttttt aattgtagtc ttgtattttg agtgtcattc
     1501 gcgtggtatt tttgtggatt gatggttttt gaaaggtttt tgaaacgagt tgaaaacgag
     1561 tttcttcttg tcttgatact atatagaaat aactcgattt taaaattata gctgtaatcc
     1621 ttggtatgta agggattata gctatttttc ttgttgaaag gaatttttga cataaaaaaa
     1681 ccccagtagt atgattaaag cgtctactca ataaacaact actggaggtc tctttatatg
     1741 caatataata ctactagatg tatagacgaa aatcaagata acgaaacact taaagatatg
     1801 acgaaaagtg ggaaacaacg cccatggaga gaaaagaaga tagataatgt aagttatgca
     1861 gatatactgg aaattttaaa aataaaaaag gcttttaatg taaaacaatg tggtaacgtc
     1921 ttagagttca agccgactga tgaaggttat ttgaagttac ataagacatg gttttgtaag
     1981 tcgaaactct gcccagtttg taattggagg cgtgctatga aaaatagtta tcaagctcaa
     2041 aaagtgattg aagaagttgt taaagaaaaa ccaaaagcgc gttggttatt tttaacactt
     2101 tcaacgaaaa atgcgataga tggggatact ttagaacaaa gtttgaaaca tttaacgaaa
     2161 gcatttgata ggttaagtag atataaaaaa gtgaagcaaa atcttgttgg gtttttgcgt
     2221 tcaacggaag taacagttaa taaaaatgat ggtagttata atcaacatat gcatgtttta
     2281 tt
//
LOCUS       DAMZLE010000234         2256 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMZLE010000234 DAMZLE010000000
VERSION     DAMZLE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.240"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            225..893
                     /gene="queC"
                     /locus_tag="POB92_002715"
     CDS             225..893
                     /gene="queC"
                     /locus_tag="POB92_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDI6580139.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            895..1314
                     /gene="queD"
                     /locus_tag="POB92_002716"
     CDS             895..1314
                     /gene="queD"
                     /locus_tag="POB92_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDI6580140.1"
                     /translation="MVQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1318..2031
                     /gene="queE"
                     /locus_tag="POB92_002717"
     CDS             1318..2031
                     /gene="queE"
                     /locus_tag="POB92_002717"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDI6580141.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT          800 a          362 c          383 g          711 t
ORIGIN      
        1 ataaatgaat atcagaggtt cctagctgaa accctctata aaaaactaga cattgaaatt
       61 tcaaacattc gttggtaaac ctttaatata tattataaga gcaacccatt tctttcgtct
      121 atcttttatg gagataggct ttttttattt tataaattaa aaacgggctt atcaatgcca
      181 ttaacctctt tattaatatt attaatgata ggagcaatta caaaatggaa agtgtattaa
      241 ataatgaaaa agccatcgtt gtctttagtg gtggtcaaga cagtacaaca tgtctctttt
      301 atgcaaaaaa acatttcaaa gaagttgaac tcgtaacgtt taattatggc caaagacatg
      361 atactgaaat tgaagtcgca aaacaaattg cacaagatca aggaatgaaa catcacgttt
      421 tagatatgtc attattatca caacttactc caaacgcatt aacacaacat gatatggaaa
      481 ttactaataa tgaagatggt atacctaata catttgttcc agctagaaat ttacttttct
      541 tgtcgtttgc aggcgctcta gcttatcaaa ttggggctaa gcatattatt acaggcgtat
      601 gtgaaacaga cttttcaggc tacccagact gtcgcgatag ttttataaaa tcaatgaacg
      661 taacattaag cctagctatg gacaaagatt ttgtcattca tactccttta atgtggttaa
      721 acaaagcaga aacgtggaaa ttaagtgatg aactcgaagt tttagattat attcgtacaa
      781 aaacattaac atgctataac ggtatcattg gggatggctg tggtgaatgt ccagcttgtc
      841 atttacgtca acgtggacta aatcaatatc ttgaaagtaa aggagcgctt taaaatggta
      901 caacaaatct atcctagtac aacgcatcca tatcaattcg aattaaataa agactttaat
      961 ttttcggctg cacatcacat tccttgtgaa gaagcaggta tttgtcaaaa tgtccatggt
     1021 catacttact ttgttaattt aacaattgtc ggtgataaac tagatgacac tggcttctta
     1081 gtgaacttta gccatttgaa aaagatgata cacggtaaat ttgaccatca actgttaaat
     1141 aacttacctg cttttaaaaa caaaatccct tcaactgaaa tcgtagcgga aacaatttat
     1201 caaattgtta aagaaaattt ggcatcgctc gaacaccaac caaaatgtat tcaagtattt
     1261 gtaagagaaa caccaacaag ttatgttgta tttagaccaa aggaacaggt gtaactaatg
     1321 gctaaaattc ctgtactcga aatattcggt ccaaccattc agggtgaagg tcgagtaatc
     1381 ggtagaaaaa cgatgtttgt gagaacagct ggatgtgatt atcgctgtag ctggtgtgat
     1441 tctgcattta cttgggatgg tagtgctaaa ggcgatataa aactcatgac cgctgaagaa
     1501 atttatgatg aattaaaacg aattggtggc gatttattta atcacgttac aatttctggt
     1561 ggtaacccag cattaattaa aggtatccaa gagttagttg acttatttca agataaaggt
     1621 attttcagcg cactggagac acaaggcagt aaattccaac cttggatgac acaaattgat
     1681 gatttaacga ttagtccaaa accaccaagt tcaactatga cacctgattt aaaaaagtta
     1741 gatgaagtga ttacacaatg cgtaccttcc tcattaaact taaaagtagt tgtattcgac
     1801 gataaagatt atgattttgc caaaatgata caccaccgtt atccagatat tcccttctat
     1861 ttacaagttg gtaacccata tttatcagac agcgtagata atcataccga aaagttgtta
     1921 gaacgctacg agcagttggt ggatttagtt atgcaaagta atgatatgaa tcacgtttat
     1981 gtgttaccac aacttcatac attactttgg agtaataaaa aaggtgtata aaggataact
     2041 tattttactt tttacaagtc atatctaatg aataacaata tattaattga tataatagtt
     2101 gattgaagta taacgaacag gagtccatca tgattttata tatattaatt aatattgcta
     2161 tcgtcgtact tataactgga ttcaatttat ataggcatca aatgcaacat ttatcattaa
     2221 gtgcaatgtt gttatctatt acgataaacg ccttta
//
LOCUS       DAMZLE010000235         2227 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMZLE010000235 DAMZLE010000000
VERSION     DAMZLE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.241"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            195..1130
                     /gene="lukE"
                     /locus_tag="POB92_002718"
     CDS             195..1130
                     /gene="lukE"
                     /locus_tag="POB92_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDI6580142.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1132..2115
                     /gene="lukD"
                     /locus_tag="POB92_002719"
     CDS             1132..2115
                     /gene="lukD"
                     /locus_tag="POB92_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDI6580143.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
BASE COUNT          878 a          308 c          378 g          663 t
ORIGIN      
        1 tataaaggga actatttaaa tgtaatgaag gttgcatcaa aatgaccggt tttcagcagc
       61 ataaatgaat aaattgtgtc atttttaata ttttatagca ttttattttt taatgaacag
      121 ttaattttat aaataagtga aataatctag aataaatttg tattaaaaat ttatatagaa
      181 agaaagtgaa gcttatgttt aagaaaaaaa tgttagctgc aactttgtca gtaggactga
      241 ttgcaccttt agcatctccg attcaagaat ctagagcaaa tactaatatt gaaaatattg
      301 gtgatggtgc tgaagtaatc aaacgtacgg aggatgtaag tagtaagaaa tggggcgtta
      361 ctcaaaatgt ccaattcgac tttgtaaaag ataaaaaata taacaaagac gctttaattg
      421 ttaaaatgca aggttttatt aattccagaa cttcattttc agatgtgaag ggtagtggat
      481 atgaattaac taaacgaatg atttggccat tccaatataa tataggactg acgactaaag
      541 atccaaatgt tagcttaatc aattaccttc ctaaaaacaa aatagaaact actgatgttg
      601 gtcaaacatt aggatataac attggaggta atttccagtc agcaccatct ataggtggca
      661 atggctcatt taattattct aaaacaatta gttataccca aaagagttat gtcagtgaag
      721 tagacaagca aaactcaaaa tctgttaaat ggggtgttaa agcaaacgaa tttgttacgc
      781 ctgatggaaa aaaatctgcg catgatagat atttattcgt acaaagtcca aatggtccaa
      841 caggttcagc aagagaatat tttgctcctg ataatcaatt gccaccttta gttcaaagtg
      901 gctttaatcc atcgtttatc actacactat cacatgaaaa aggttcaagt gatacgagtg
      961 aatttgaaat ttcatatggt agaaacttag atattacata tgcgacttta ttccctagaa
     1021 ctggtattta cgcagaaaga aagcataatg catttgtaaa tagaaacttt gtagttagat
     1081 atgaagttaa ttggaaaaca cacgaaatta aagtgaaagg acataattaa tatgaaaatg
     1141 aaaaaattag tcaaatcatc agttgcttca tcaattgcac tgcttttgct atcgaataca
     1201 gttgatgcag ctcaacatat cacacctgta agcgagaaaa aagtagatga caaaatcact
     1261 ttatacaaaa caacagcaac atctgataat gataaattga atatttctca aattttaacg
     1321 tttaatttca ttaaggataa aagttatgac aaagatacgt tagtacttaa ggcagccgga
     1381 aacattaatt caggttataa aaagcctaat ccaaaagatt acaattactc acagttttat
     1441 tggggcggta agtataatgt ttcggttagt tcagaatcaa atgatgctgt aaatgttgtt
     1501 gactatgcac ctaaaaatca aaatgaagaa ttccaagttc aacaaacatt aggttattct
     1561 tatggcggag atattaatat atctaatggc ttatcaggtg gattaaatgg atcaaaatca
     1621 ttttcagaaa cgataaatta taaacaagaa agttacagaa ctacgattga tagaaaaaca
     1681 aatcataaat caattggctg gggtgttgag gcgcacaaaa ttatgaataa tggttgggga
     1741 ccatatggta gagatagtta tgacccaaca tatggtaatg aactgttttt aggtggtaga
     1801 caaagtagtt caaatgctgg tcaaaatttc ttgccaacac atcaaatgcc tttattggcg
     1861 cgtggtaact ttaacccaga atttataagc gtactttctc ataaacaaaa tgatacaaaa
     1921 aaatctaaaa tcaaagtaac ttaccaaaga gaaatggata gatatactaa tcaatggaat
     1981 cgactacact gggttggtaa taactacaaa aatcaaaata cagtaacgtt tacatctact
     2041 tatgaagttg actggcaaaa ccatactgtt aaattaatcg gtacggattc taaagaaact
     2101 aatcctggag tataactgtt agaaatagag gaagttaaac ccgaaattta agagttaaac
     2161 aaaaaatatt cacaacatat taaaaaaaga agttacatta tttgtttaaa ctaacaatag
     2221 taccata
//
LOCUS       DAMZLE010000236         2212 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMZLE010000236 DAMZLE010000000
VERSION     DAMZLE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.242"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            54..1436
                     /locus_tag="POB92_002720"
     CDS             54..1436
                     /locus_tag="POB92_002720"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI6580144.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(1490..2065)
                     /locus_tag="POB92_002721"
     CDS             complement(1490..2065)
                     /locus_tag="POB92_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580145.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
BASE COUNT          795 a          327 c          351 g          739 t
ORIGIN      
        1 acttacaaaa ctgaatacaa ttttctgatt ttttagaaag gttggcatgc actatgaaag
       61 tttccaaatt cggtggtagc tccgtctcca atgctactca aattaaaaag gttttaaata
      121 ttgttaattc tgatccagag cgaaagattg ttatcgtttc tgctccaggt aaaagacatg
      181 ataatgatat taaaacaact gatttgttaa tcagattata tgaaaaggtc attaatcatc
      241 ttgattatca tgataaaaaa agagaaatta ttcagcgtta tgatgatatt gtaaaagaat
      301 tgcaaatgga tgaaagtatt ttacggacga tagatgtgac tttggaacat tatattaatc
      361 aattaaaaaa tgaaccaaag agactattag atgcattact ttcttgtggt gaagatttta
      421 atgcgcaatt gatagcttta tataataata gtcaaggtat accaacaaaa tatatatcac
      481 cgaaggaagc gggtattttt gttactgacg tcccacaaga agcacaaata ttagatagtg
      541 cttactctga aattaaaaaa ttaagagatt atgatgagaa actaattatc ccaggatttt
      601 tcggcgtatc tcatgaagga tatatagtta catttccacg tggcggatca gatataactg
      661 gcgccatcat atcaagtggt gttagagcta caatttatga aaatttcacc gatgtgtctg
      721 gtatttataa agctaatcca aatatcatca aagatcctga attaatcgaa gaaatcactt
      781 atcgagaaat gcgtgaatta tcttatgctg gttttagtgt tttccatgat gaagccttac
      841 aaccacttta taaagatcgc atccctgtgg tgattaaaaa tacgaaccgc cctcaagata
      901 aaggtacatt tatagtgcat gatagagaaa taaatgcgaa aaatgtgatt agcggtataa
      961 gttgtgataa agattttacc gttattaata tcaaaaagta tttaatgaat agacaagtcg
     1021 ggtttactag aaagatatta ggtgtcttag aggataataa tatttcattt gatcatatgc
     1081 cttcaggtat tgatactatc agtatcgtta tgcgttcaaa acaaattcaa aataaagaaa
     1141 caaaagtatt aaatgaaatt cgtcaaaaat gtgatgttga tgagctaagt attgagcatg
     1201 atttagcaat attaatgatt gtcggcgaag gtatgaataa agtcatcggt actgcaaata
     1261 aaattacaca tgccttagct gaatcaaaaa ttaacttaaa aatgattaac caaggtgctt
     1321 cggaaatctc gatgatgttt ggaattcatg tagatgatgc tgaaaaagct gtattatcta
     1381 catatgagtt ttgctatcac ggggttgcat taaaaaactt gtgtgataaa aactaatata
     1441 aaagcaattc gtaacacatg tattcaactg cgttatgaat tgctttttct taattattta
     1501 ttgttctctg gattgcatcc ttaatacgtt gctcacctac tacaaatttt aaaataaaaa
     1561 attgatagta gcttaatgca tctatgactc gtttataatt ggaataatga tgatactgat
     1621 ggtgttgcat tagatcatac attagaacaa tctccggttt aatataatcc acatcccaat
     1681 ttaaactatg gaagtatatg ttactcttag gtatacgtat attcggatcc aatctaaaaa
     1741 tccattcatc gtttactaca tcataaacga aaatactcat tattgttgta tcatcatatt
     1801 gcacataaac ttttgcaatt tttgaaagtg ataaatcttc tttagctatt gcttgatctt
     1861 gcttatctat gtatagtaac gcatatgatt gcggaataac tttcacaata tcacttaaac
     1921 gcttacgttc cacgcatatt tcaacaatag aaggtaattt taattcatca tttaagaaaa
     1981 gctgcatggc aacttcaccg tgaaatttaa ataattgcga tgatgttttc attaaattgt
     2041 ccactaattg ttctttagca ttcatttcat tcactctcaa atacacctcc taagcaagcg
     2101 ctttcacaaa tatcattata cattttaaaa ttaatgaatt caatcttcaa aatactatga
     2161 gtgacctaat tctgtcattt aaatgaccgt tataacataa attcgacata aa
//
LOCUS       DAMZLE010000237         2160 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMZLE010000237 DAMZLE010000000
VERSION     DAMZLE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.243"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(162..1169)
                     /locus_tag="POB92_002722"
     CDS             complement(162..1169)
                     /locus_tag="POB92_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDI6580146.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1185..1802)
                     /locus_tag="POB92_002723"
     CDS             complement(1185..1802)
                     /locus_tag="POB92_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDI6580147.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          744 a          382 c          303 g          731 t
ORIGIN      
        1 ttccatggaa taaatttata acttagaccc ctaattaaaa tgctttgtct tggtcattct
       61 acgtaaactc tataaaatat gttatctact tacataaaaa actgaacttc aataccacca
      121 tatgtttgtg atactgaagt tcagtttagt tttattttca attagaaaaa taagttaagt
      181 atatagaata gtaaacctgc taacaatgct gaaataggta atgtaatcac ccatgtaatg
      241 atcattcgtt gcgcagtgct ccattttaca cctttagctc ggttagaagc accaacacct
      301 aagattgatg atgacacaac gtgagttgtt gataatggga aatgtagcga tgatgcaaca
      361 aaaattgtta atgcagatga taaatcggcc gcagcaccat ttgctggacg tattttcata
      421 atattaccac ctacagtttt gataattttc cagccaccaa ttgcagtacc aagccccatt
      481 gctgtcgcac aggcaaattt tacccataac tgtggttcaa cgctgccatc attctgtaca
      541 ttagcaacaa ttaatgccaa cgtaataata cccattgact tttgcgcatc attcgtaccg
      601 tgagagaatg attgtaacgc tgctgtgaaa atttggaaaa atctaaagtt acgattcgct
      661 cttgttaaat ttgcattttt aaagataact ttaaaaattg aatacatcaa gaaaccaaca
      721 caaaatgcga ttatcggtga aacgattaat acaataataa tttttgtgaa accttggtaa
      781 tgtaacactc caaatgagcc ttcagatgcg attgctgcac ccgcaattga acctataagt
      841 gcatgtgaag acgaacttgg aattccgtaa aaccaagtag ctaaattcca aataatagcc
      901 gcaagtattg cagctaacac aacaactaat ccattttcca atttaaatgg atcgacaatg
      961 tctttagtaa tggtgcctgc aacgcccgta aatgttaaag cacctataaa gttcatcact
     1021 gctgccatta aaattgccgt tttaggcgtt aacgctctag tagatacagc agtagctact
     1081 gcattggctg tatcatggaa tccattgata aagtcaaata tcagcgagaa aataactaca
     1141 gctatagtga cgatgattat atatgacata aatatatact ccccttagct atttttcata
     1201 ataatagttt caaaattatt tgctacgatt tgacatttat cagcgatttc ttccatgctt
     1261 tcataaatat cttttatttt aattaaagtg attggatctg tttcgctatt gaaaatatgt
     1321 ttaattgact gtcttaaaat accatcacag tttgtttcaa attctttaat attaattgaa
     1381 tgaatacgca tatgtgataa ttttttatcg actaataagc cgacagcaag tttcatttct
     1441 gcaactgctt tttgaatgtt atcaacaaac tcagccatat attcatctgt gtattcgatt
     1501 gaatacattt caaacatagc tgccgtttct tcaattgcat ctaaaacatc atcaattgca
     1561 tcacataatg ataaaatatc ttcacgttca attggtgtga taaatgtttg atttaaatca
     1621 gtaattactt gatgtactaa ttcgtcacca tgtgactcat aagttttaat gttgtctgag
     1681 tatgctttta aatctaaatg tgtattgaaa tccattttac cgaattcaat agcagcacga
     1741 tccagattga aaaccatctc ttctaattga accataaact tatctttttt cttactaaac
     1801 atttaaaacc ctccatttaa gcgattgtca ccaatcacat tcagttataa tttgtttcaa
     1861 attaagacaa gtgaatttac aaactaatga tacaaatttg ttattatcaa tcgtcagtat
     1921 aattttagtg tactgatatt aatttcaaaa atgcctcaca gtaaacaatt tactgtattt
     1981 gcccttatat gaaaatagca caaaatttac aatcccacaa ttcaatttac aaatttttaa
     2041 caattccgaa acttaacttt tacataaaat ttaaatagta gtaggcattt gttttgaaaa
     2101 agttattgta taggcaattt tttatctata caactctaaa aattgtaaat ggatatagtt
//
LOCUS       DAMZLE010000238         2141 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMZLE010000238 DAMZLE010000000
VERSION     DAMZLE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.244"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            320..496
                     /locus_tag="POB92_002724"
                     /pseudo
     CDS             320..496
                     /locus_tag="POB92_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(474..575)
                     /locus_tag="POB92_002725"
     CDS             complement(474..575)
                     /locus_tag="POB92_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580148.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(698..793)
                     /gene="pepA1"
                     /locus_tag="POB92_002726"
     CDS             complement(698..793)
                     /gene="pepA1"
                     /locus_tag="POB92_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDI6580149.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            996..1139
                     /locus_tag="POB92_002727"
     CDS             996..1139
                     /locus_tag="POB92_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6580150.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1743..2126
                     /locus_tag="POB92_002728"
     CDS             1743..2126
                     /locus_tag="POB92_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580151.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     VYIKLKTKKNAAGEYVLTGEKWGYQ"
BASE COUNT          820 a          290 c          370 g          661 t
ORIGIN      
        1 agatctgttg atgattttca atttgatacc gatatttctt atacagatga attgggtaga
       61 aaatttaata aaaatcctaa gtcagtttct gaaataaaaa aagagcaaaa tacgtccaat
      121 aaataattgt tcatattgtg atgaaacaaa aatataagtc attagatgag tttaactatg
      181 ttataaatat ttgtagtatc tataaaaatc tcgacactat taaaatgata aagtgccgag
      241 gttttcttac ttatttagtt aattcaaagt ttatgccaga ttcataagaa tttgtgacac
      301 ttttaatagt gtaaccattg attattacaa tttatcaaat ggtcctttag aaggtataaa
      361 taacaaaata aaattaatta aacgtacatc ttttggttat ggaagttaca atcatttgcg
      421 caatcgaata ttattatgtt caaaacttta cgctccaaaa agtaaaaagg aagttaagca
      481 atgtttagtt gcttaacttc ggatattgaa cgcatcagtc caatttgaca tagagccttt
      541 tttagttctt gatgtttctc tttaaaacct tgcatatttt acaaaaagaa aaattagcag
      601 tataattaag acaacgaaaa taagtattta cttatacacc aatcccctca ctatttgcgg
      661 tagtgagggg atttttattg gtgcggctat atgtcaccta ttttgtattg cgtctactta
      721 gccaataaga aaaaaacgca atggcacagc cactgatgac tggtgctatg atgtgaacga
      781 aaataagcat caccttatac acctcctctc tgcgtctaaa ttgacgcctg agagataggc
      841 gactctacta ttatatcatc ggcaaatata caagcacagt cacttgcttc tgataagtta
      901 tatgattcta gctgatagat tgaatcgtct acacttaatt ggacaaattc tatgagaata
      961 gatattgtta atttaagaaa gtaggcgatt ttattatgac aagagaaaga agatcattta
     1021 gttcagagtt taagttacaa atggttagat tatataaaaa tggtaagcct aggaatgaaa
     1081 ttatacgcga gtatgatttc acaccttcga cgtttgtaaa tggcggttat aaaatgtagg
     1141 aaaatggata aagcaacatc aaaacacggg tacattcaat caccaagata acttatcgga
     1201 tgaagaaaaa gagctgatta aattacgcaa agaagttcaa catttaaaaa tggagaacga
     1261 tattttaaag caagtagcgc tgattatggg gcaaaaatag aagtcattca aaagaatgca
     1321 catcaatatt cagtatcagc aatgtgtaaa gtcctgataa tactaagaag tacctattat
     1381 gattctataa aaagaaaaga taataaaatc actaaagatg attcaaacat agaacatgcc
     1441 gtcataaata tttttaattc taatagaaaa gtctttggta caagacgaat taaaaatcat
     1501 ttaaatgaca agggtctcac tgtatctgga caaaagatag gtcgatcatg aaaaaatcta
     1561 gtttctgttt atacgaaatc taaatacaaa aatcatctaa aagaaactaa tgaaaaacga
     1621 attaaaaatc tttattatta gctgctggtg tattatttgt tagtccaatt tcattatctt
     1681 ataattcaga tgtagctcat gctgaagata agttagacca ttctcaagca aaggtaatat
     1741 atttgagtaa ccaaaattta tttgatgaac ttgagaaaaa aggttataaa ctggaagata
     1801 tatttacaaa agaagaaata aaaaaatata aagctgaaga ccaattaaga gcgggtaaaa
     1861 ctcaatatgt agaaacaggt aaagatactg caacattata tctttcttct gcatatacaa
     1921 aaacaatagc tgctttaggt gcgggggcta ttagcgttat ttctgcacta acaggtggtt
     1981 tagttggcgc aggtgttggt ggtttccttg gatctatagc tgcatcgaat attgatacta
     2041 gtaagggagt atatataaaa ttaaaaacta aaaagaatgc agccggggaa tacgttctga
     2101 caggagaaaa atggggatat cagtaagggt gattttatgg a
//
LOCUS       DAMZLE010000239         2127 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMZLE010000239 DAMZLE010000000
VERSION     DAMZLE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.245"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(265..2073)
                     /gene="pepF"
                     /locus_tag="POB92_002729"
     CDS             complement(265..2073)
                     /gene="pepF"
                     /locus_tag="POB92_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI6580152.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT          651 a          373 c          291 g          812 t
ORIGIN      
        1 tataaaaagt gcatatttaa tacattttta tacaacttca cttccctctt attaaaaaag
       61 aacataaaaa aacgtcgtgt ctgaatacac gacgctaaac aaaggggatg ggagaaattt
      121 ttcacttcaa acaaaggggt atgtttgtta tgtgattaat ttcatgttca taatataaca
      181 tgctatctct accatttcaa ccttttaata gtacttaatt taaattgtca caatctgttc
      241 ataatgtaag cgaatacaat agtactaagc tttcattaat ttttcaaaag cgttcaattt
      301 ttgttcaaaa acttcacaag cttgttcaat tggttcaggt gttgtcatat ctacaccagc
      361 attttttaat atctcaattg gataatttga gctacctttt ttcaagaatt cattaatata
      421 tctatctact gctggcttac cttctgttaa aatttgatga cttaagcttt gagctgcact
      481 gtaaccagtt gcgtattgat atacataata attcatatag aagtgtggaa tacgtgacca
      541 ttccttacta atatcttcat cagtttctac agaatcaccg aagtataatt tatttaattt
      601 ggcatattct tcattcattc tagttggtgt taaaggttca cctgcttctt caattgcatg
      661 aattttatgc tcaaattctg cgaacattgt ttgtcggaat aatgtagctc tgaaacgttc
      721 taattcttgg tttaataata ataagcgttt ttcatcatca agatgtttat ccatataatc
      781 acttaaaagt gcttcgttac aagttgatgc aacttcagcg acaaaaatag tgtagtcact
      841 agaatttgac ggttggaatt ttctactgaa gtaactatgt gctgaatgcc caaattcatg
      901 aactaatgtg tataagtctg aaatagtatt agaccagtta agtagaataa atggattagt
      961 taaatgtgca cctgatgaat agccacctga acgtttacct ttattctcat agacatcgac
     1021 ccaacgattg tttaagcctt ctttaactac atttaaatat tcttcaccca ttggttctaa
     1081 agcttttaac atccactctt tagcttcttc ataaggcatt tcaaacttaa tatctttaat
     1141 taatggtgta tataaatcat acattttcaa gtcatctaaa cctagcaatt ctttgcgcaa
     1201 ttcagtatat ctatgtagca atggtaaata tttatgtaca gtttttacta gattgtcata
     1261 tacattttct ggaatatgat tattactcaa tgctttttct cttgcagttt tgtaattgtg
     1321 tgtacgagca ttaaatacat ttttcttcac ttcacctgct agcgtagcgc caagcgtatt
     1381 attatgagca ccatatgctt tatatacatt tctaaaagca ctttctctta gtttgcgatc
     1441 atctgattct aaatacttaa taaatgtacc ttgtgttaac gggtgtgcat ttccatcttt
     1501 atcaatcgca tcttcaaata ctaaatcagc gttgctaaac ataccgtata catttgatgg
     1561 cgttgataac gcgtcctgcg cttctgttaa taacttttca gtttcagcat ctaaaatatg
     1621 aggacgtttt tcattaatca acttcaaatc aaatgcgaat ttttgtaatt tatcatatga
     1681 atttacaaat gattgaattt tatcttcatc aatttgtaaa atctctggca ctaagaaact
     1741 ccatgccgag ctaaatttaa taattaattg atgtgctctt gactccatac cagtatactt
     1801 gtcgttcgtt gtatcttggt cttgttttag gtgcgcgtat acatatactt tttctaattt
     1861 agtacctaat gtatcttcta attctaatgc attgtataat gtctcagcac tatcaccaat
     1921 gtgtccttta aattgttctt ctttgcctaa ctcattttca acttctttaa atgcagcctc
     1981 aaaagcttca tcatctttga aaattgttgt taagtcccat gtatattcag gatatttacg
     2041 ttcctgttct tctctagata attgttgact catatgtagt aaacctccta ataaaatata
     2101 attgatataa ttttctcatt ttagaac
//
LOCUS       DAMZLE010000240         2114 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMZLE010000240 DAMZLE010000000
VERSION     DAMZLE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.246"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            120..323
                     /locus_tag="POB92_002730"
     CDS             120..323
                     /locus_tag="POB92_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6580153.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(320..556)
                     /locus_tag="POB92_002731"
     CDS             complement(320..556)
                     /locus_tag="POB92_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580154.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            780..1361
                     /locus_tag="POB92_002732"
     CDS             780..1361
                     /locus_tag="POB92_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6580155.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1410..2027)
                     /locus_tag="POB92_002733"
     CDS             complement(1410..2027)
                     /locus_tag="POB92_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDI6580156.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     AAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGEMVGSIDKTGKLLTIINKLSSIIIIIVALMILQKLIQLLF"
BASE COUNT          777 a          328 c          323 g          686 t
ORIGIN      
        1 tagaatcaga taacaaaaaa tgtgcaaaat cacttttgca catttacaaa tactagtttt
       61 ctgataagat taataataac actttaaata gcgcttaata aatgaagggg gcaagtctta
      121 tgacgtttta caatttcatc atgggttttc aaaatgataa cacaccattt ggtatattgg
      181 ccgaacacgt tagtgaagat aaagcattcc ctcgattaga agaaagacac caagtaatta
      241 gagcatatgt gatgtctaat tacacagatc atcaattaat tgaaactaca aatagagcta
      301 ttagcttata tatggcaaat taatttgagt agtaccaatt atgatgtatt agtgcatccc
      361 aaatatcttt tgttttaaag tttatttcat catttcttat cgaaaatggt gtaataatgt
      421 ctttatctaa ccaagtgttg ataagttcat ttggtacacc atctaacaac atttcacttt
      481 tactaattat aaaacattcc cagtcaagtg aaacattttg tggattcaca taattacatt
      541 gattatgatt atccataaac actcactcct ttaaaattct gtactcttca ttgcatttta
      601 ccccgtcaca ttatctttta aactaaaatc atcattactt atgaaaaaat gtacatcaaa
      661 agcaaaggtt ttcgctaccg aaaaagttta aataatggtt taatatattt ggtactcatt
      721 ttaataaaaa gagaatacat tttgagctat caatactttt tattgaagag gtgttattgt
      781 tggctaaaac gttatattta atgcgccacg gacaaacttt gtttaatttt aagggactaa
      841 ttcagggatt tggagattcg ccgctaacag aacttggaat tgctcaagct caaaaggcac
      901 gtagttatta tgaaactaag gggataaact tcgatttata tgcatcatca acgcaagaac
      961 gcgcaagtga cacacttgaa aatgttgcac ctaaccaatc gtatcaacgt tttaagggac
     1021 tgaaggaatg gcattttgga ttatttgaag gtgagtcagt ctatctattt gataatctat
     1081 acaagcctga agacttattc ggagatcgaa ttgttccttt caaaggagag gcaaggcaac
     1141 aagttgaaga tcgcattgtg aaaactttac atgacattat gtctcaaaca aagaataatg
     1201 cattagtcgt gagtcatgga acaataatgg gagtattttt aagatattgc cttaaactag
     1261 atgaagcatt aaagcataat atcggtaatt gtaatatcct gaaatttgaa tatgacaatg
     1321 gaacatttaa atttgttgag ttaattgatc caaatttata ataaataaag cgttatacat
     1381 agacacaagc atgtcttttg tataacgctt tagaataata attgaataag tttttgtaat
     1441 atcatcagag caacaataat aattattatg cttgataatt tattgattat tgttaataat
     1501 tttccggttt tatcaattga tccaaccatt tctcctaaaa ttgcgagtaa aaagaaccat
     1561 aaccatgaaa cgctaataca agcgattgta aacgcaattt tattgctgcc actatataat
     1621 gcagcactac taccaattac accaattgta tctaaaatag catgtggatt gagtaatgaa
     1681 actgataaag caaaacttac ttgtttcatt ggagacataa tttgagcttc tccatctgtt
     1741 gagggtttat catgccaaat ggtccaagcc atatacatca agaaaattaa accaactata
     1801 taaataattg cttgaagtac aggtaaagac ataataatga tagatactcc taccactgca
     1861 ataataataa gtaagctgtc tgacaacccg gctgcaatta ttgcaggcaa tacatatcta
     1921 tattttggtt gattagctcc ttggttaaaa ataaatacat tttgtgcacc taaaggtaaa
     1981 attagaccaa tcgctaagat aaatccatga ataattgcgg ttaccattaa gttttgacac
     2041 ctctaaaaca tagaatattc tgaaaaaact tattgttatc atatcaaata aaaaacaact
     2101 actcaatagt agag
//
LOCUS       DAMZLE010000241         2058 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMZLE010000241 DAMZLE010000000
VERSION     DAMZLE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.247"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            193..699
                     /locus_tag="POB92_002734"
     CDS             193..699
                     /locus_tag="POB92_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580157.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            958..1455
                     /gene="efb"
                     /locus_tag="POB92_002735"
     CDS             958..1455
                     /gene="efb"
                     /locus_tag="POB92_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDI6580158.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1609..1959
                     /gene="scb"
                     /locus_tag="POB92_002736"
     CDS             1609..1959
                     /gene="scb"
                     /locus_tag="POB92_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDI6580159.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
BASE COUNT          805 a          241 c          321 g          691 t
ORIGIN      
        1 atttagttga attatgcatt ttcgaagtaa gcataattca acacttacat ttttaaatag
       61 ataaaatagt gtaaaatcac gtgccaagat tactatagag taggcaatga gtattattgg
      121 caatcactta agtgattgtt attttttcgt ggtcaataat aaagatataa ataaatatat
      181 tgaggtcaaa cgatgataat taaaatttta acaattctat tactactttg tatattgagc
      241 tatttggtta caaatagaaa gaagcctttt ctgttcttaa aaacactctt tatgggtgtg
      301 gtatttatct ttataggata tatttcactg gcaatatctg ccgtaattat ttatggcatt
      361 attcaattta tcacaattga ttttggtagt tttttcttaa tgggtattat attaatcttg
      421 atttcaagta tattccaatt atttatagtt agattacttt ttagaaaaaa gaatgtcgat
      481 ttgacagagg ttgtcgtttt agaacattta attcaatggt tcttagttta ctttgcgatc
      541 tatcaagcag taaatgaaaa aatggacatt aatgatatta atatcgacaa ttttcaatct
      601 gtcttttttg acgtgtctaa tttgaattta gtaattctac caacgttaat cattagctgg
      661 gtcacaatat ttaactatag aatgagaagt tacaaataaa atcaatgaaa ttataccatc
      721 agacaccaac attcaaatgg tgtctttttt gttgtgtggt tttatttttg aaattcgaaa
      781 aagtagaggc atgaattttt tgactagtgt ataagtgctg atgagacaca agatagataa
      841 ctatattttg tctatattat aaagtgttta tagttaatta ataattagtt aatttcaaaa
      901 gttgtataaa taggataact taataaatgt aagataataa tttggaggat aattaacatg
      961 aaaaataaat tgatagcaaa atctttatta acaatagcgg caataggtat tactacaact
     1021 acaattgcgt caacagcaga tgcgagcgaa ggatacggtc caagagaaaa gaaaccagtg
     1081 agtattaatc acaatatcgt agagtacaat gatggtactt ttaaatatca atctagacca
     1141 aaatttaact caacacctaa atatattaaa ttcaaacatg actataatat tttagaattt
     1201 aacgatggta cattcgaata tggtgcacgt ccacaattta ataaaccagc agcaaaaact
     1261 gatgcaacta ttaaaaaaga acaaaaattg attcaagctc aaaatcttgt gagagaattt
     1321 gaaaaaacac atactgtcag tgcacacaga aaagcacaaa aggcagtcaa cttagtttcg
     1381 tttgaataca aagttaagaa aatggtctta caagagcgaa ttgataatgt attaaaacaa
     1441 ggattagtta aataaaactt caatcgttgc tgttatctgg aaataattaa ttaaatgtta
     1501 tgttaatttt tgttaatgaa aaaagtaatc tatttaatga caggttaatg taattgtcct
     1561 gaaattgact atatactcag taagtatcaa ttttaaggag agcttataat gaaatttaaa
     1621 aaatatatat taacaggaac attagcatta cttttatcat caactgggat agcaactata
     1681 gaagggaata aagcagatgc aagtagtctg gacaaatatt taactgaaag tcagtttcat
     1741 gataaacgca tagcagaaga attaagaact ttacttaaca aatcgaatgt atatgcatta
     1801 gctgcaggaa gcttaaatcc atattataaa cgtacgatta tgatgaatga atatagagct
     1861 aaagcggcac ttaagaaaaa tgatttcgta tcaatggctg atgctaaagt tgcattagaa
     1921 aaaatataca aagaaattga tgaaattata aatagataat aaataaaaca ggttgagaca
     1981 aaaaatggtc ttaacctgtt ttcaatttgc atatgtgata aattctatat caaaatgctt
     2041 atgtataatg aatgacat
//
LOCUS       DAMZLE010000242         2057 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMZLE010000242 DAMZLE010000000
VERSION     DAMZLE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.248"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..294)
                     /locus_tag="POB92_002737"
     CDS             complement(<1..294)
                     /locus_tag="POB92_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580160.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYS"
     gene            complement(418..1047)
                     /locus_tag="POB92_002738"
     CDS             complement(418..1047)
                     /locus_tag="POB92_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDI6580161.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(1500..>2057)
                     /locus_tag="POB92_002739"
     CDS             complement(1500..>2057)
                     /locus_tag="POB92_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580162.1"
                     /translation="PKYRVIRYNNEYFMVDLVSTWITYFFAMINWFLPKKYAKISENE
                     FERLNIVEPVKNNVFWPVAGSSVLFGIILRKYGNFFNVQFEKQLAITVFFIMLIGMLI
                     FNTNVVNKNRVVLIPTFKQGLLIVFAYFFLGSASIFTLTILLTTESQNIIIFLTWVII
                     SMFFFLVNMASIGNKNVHVILRINE"
BASE COUNT          750 a          353 c          224 g          730 t
ORIGIN      
        1 actatattca tatttaacga taaataaatg ggaatacctt cttaacataa caccaaataa
       61 gaccgtactt cctgccactg gccaaaaaga attgatttta gctgtcttca caatgtttaa
      121 agtttcaaaa tctttttcgc tgattttgac gtactttttt ggaatcagcc aattaatcat
      181 tgggaaaaag agtgctaacc atgtacttac taaatcaatc atcaaatatt catctttgta
      241 tttgataatt ctatatttgg gattcttatt aatattttca gtttcacaaa gcaatgtctc
      301 cacttctttt caattggttt tatattgata cactttcata tacattagaa gtcatttaaa
      361 ttctactagc gtcttaaaag gattgtgtcg acaacattta gaatatttta aatgtaatca
      421 attatttttc attataacgc gaactttttc attacctatc gaagtcatat ttaacaaaaa
      481 gaaaagcatt gtcatcataa tccaataaat aaatattatt atattttgag gttcgattgt
      541 cataagggca attaatgaaa atattgaaaa acttccagcg aaaatatatc cgaaaactaa
      601 aaaacagcca agtttaaacg ttggtattaa tataactctc ttattttttt caatatttgt
      661 atcaaacact tttaacttaa gctttctatt taagttaaaa taaaaaacta taacacaaat
      721 taaagcgaga gcacacaata tcattacaac tagcttatct aattgaacat caaaaacatg
      781 tatgtatttc cttaaagtaa ctccaaacaa aaccgaactt cctataactg accagaaaac
      841 attattttta tttggtttaa caacatttaa attttcaaat tcttcttcgc taattatcgc
      901 gtactttttt ggaatgaacc aattaatgaa aggaaaaaag tacacgaacc aagtgcttac
      961 taaatcgact aataagtatt caccgttatg ttgaatgact ttgtatttcg gatttttata
     1021 aatgactctg acatcgcaaa gcaacattta ccactcccgt ttcattagtt ttaaatgatc
     1081 cccaatactt tttgtacctt caccaactat cgttccaaca ctatctacag ctttttctgt
     1141 ggtattttta aacagcgtct atttaatgtc ttatccaatt agttttatct cttaatccaa
     1201 atttattagt atgaaaagtc caatttttaa gaattgtcta aacagattcc gcaacaaaac
     1261 cagctaaaac cgtccatact attggattaa aaacgccaat gttataagag ctatcataca
     1321 aaataacacg ctctatattt catttttaaa ttaaaacagt tttttccttt atcaatcaca
     1381 atgggcattt tcacttggta tattcaaaca ttactatcta ttttcctgat ttaagatatg
     1441 attatatttt tttgaaccac cgtttaaata tgcgatacta ccaatttcga tcatttttac
     1501 tactcgttta ttcttaaaat aacatgaaca tttttattac ctatcgaagc catattcact
     1561 aaaaagaaaa acatcgagat aataacccaa gttaaaaata ttattatatt ttgtgactct
     1621 gtcgtcaaaa gaatggttaa agtaaatatt gaagcgcttc ctaaaaaaaa gtacgcaaaa
     1681 actattaaca atccttgttt gaaagtcggt attaatacaa ctctattctt attaaccacg
     1741 ttggtattaa aaattaacat ccctattaac atgataaaaa atacagtgat tgctagttgt
     1801 ttttcaaact gaacattaaa gaagttaccg tactttctca atataattcc aaatagaact
     1861 gaacttcctg caaccggcca aaaaacatta tttttaacag gctcgactat atttaacctt
     1921 tcaaattcat tttcgctaat ttttgcgtat tttttgggca aaaaccaatt aatcatagcg
     1981 aaaaaataag taatccaagt acttactaaa tcgaccatga aatattcatt attatatcta
     2041 ataactcgat atttagg
//
LOCUS       DAMZLE010000243         2038 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMZLE010000243 DAMZLE010000000
VERSION     DAMZLE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.249"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            69..296
                     /locus_tag="POB92_002740"
     CDS             69..296
                     /locus_tag="POB92_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580163.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(602..1549)
                     /locus_tag="POB92_002741"
     CDS             complement(602..1549)
                     /locus_tag="POB92_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDI6580164.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATSIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLILAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            1798..1986
                     /locus_tag="POB92_002742"
     CDS             1798..1986
                     /locus_tag="POB92_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6580165.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
BASE COUNT          780 a          258 c          350 g          650 t
ORIGIN      
        1 tgtaaacagt ttttgtaaca catgatacat atggttgtga aaatagcata tataaaggat
       61 ggctataaat gacacatttg acaaaggttt tagatacact aactggaata tgcgtagtat
      121 tattatttag taaatatttt gtggcgtatg caaatatggt gtttgattgg aatttaagat
      181 ggtatttgct agaaaacata ccacatttgc caattatatt atttattctg atgtttattt
      241 tcggagtacc ttctgaaatg ataaaagata ggcaaaggaa aaataacggc gtttaattta
      301 tcgatattta gaggtgataa aatttgtcaa ctattacaaa gactaaaata aaaatcttta
      361 ttaattattt tattggtata ttagccttgg cgtttgctgc ttatatatta tttaaataaa
      421 ggttagagtg aaacgtgttt atgaactaga cgttctagta tagttactac agctttagtc
      481 agaaggtatc attgataaga tcatattaaa tcaaagaggc attgatatac actaaaaaga
      541 ggcaagatta cctgcctctt ttttagttat taaatatgcg tgttaattct tggtaatagt
      601 gttactgaga cgcattacga agaaatttat cttgtttttc cttttttaaa aagaagtgaa
      661 ggtatcctat aaagactcta agtactatta cagtggctaa taacacttcg ataaacaaaa
      721 gacctttcca aatgtctgga aacataagta caggcaaact gttctttaaa gcagttgctg
      781 agattactaa agggaatgtg aaagctgaaa atacgggcga aaaaggttct tttagtaatt
      841 taggcaattg tataatgata taaaaataaa atatttgagc taatattaaa aatataatta
      901 cgataaaagc attcgcctta ggaaaggcta taacatatgc tgcagcgact aaagaaaatg
      961 gtgcacaaat tgtcgatgtg ttcggtttga ttgacgtctg caatggaaat gcttttaatc
     1021 gcttgaaaac tataggtaag acaatagaag tagctacaaa gccatatatt actgttaatt
     1081 gacctatgaa aaaatatccg ctaacgggtg ccgtcaatcc tgcgatagca ataccaataa
     1141 aaagtacagt ccacgaagga taaacatttt caagtgaaaa atcttttaaa tattttattg
     1201 aaaaaataat catatgcgtc ataattccca caaggcataa aatccaaata ggcgttatta
     1261 agctattgat aaaagttatg ttactaaaaa atgtatttaa ataagtagta cctaaaaagc
     1321 cagacatgaa aaatgttgtg aacactgatg aaactagagg actgtttaat tgttctttca
     1381 cattattaaa atatttgatc atagtacata aaaggtgaat ccaaatcaag aaagcaaaga
     1441 ttccgcaaac agcgtttaaa gtaagagata agtcttttaa tagattaccc aggcctaata
     1501 atcctaagac tagtcctgac gttactagag gtgctttttg aagtctcatg atttacaacc
     1561 tttcttatgt gattttcttc actaattata tcatgatagc tatggccaat taataagaag
     1621 agtgtgtact attacgttat tagagtatgt atattggatt gatagacaca taattgacat
     1681 ttaaatctca aaattaatga tatttgaggt atgctttgca acctaaatat tggaatatgt
     1741 ggaaaagtaa ttatttaatt taaatgatga ttgaagattt acaagggggt gtacaaaatg
     1801 agaaatcaaa ttcaaaaact attagacagt gatttgagca gtttacatat atcgaaacaa
     1861 acaggagttc cacaaagcac aatacacaga atgagaaaaa atgaaagatc attagacaat
     1921 atgtcattga aaaacgctga actactttat aaatttgcca atagtatatt tagcaatgaa
     1981 aattaagaag aataattagt gagttaaata attaaaaaac cacgtctcga tggacgtg
//
LOCUS       DAMZLE010000244         2033 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMZLE010000244 DAMZLE010000000
VERSION     DAMZLE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.250"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..692
                     /locus_tag="POB92_002743"
     CDS             <1..692
                     /locus_tag="POB92_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580166.1"
                     /translation="IFKNIDHDFRDGVSEEMMKHLKVVKQNIEQIKNQNDIYGRQIAD
                     IRSIMKQQDATILDGNYQINYSGENMVQGLALPSNYLGRKMKILKDHIDDGIKKIADY
                     VQGIYDEYASKIVDVIKYLINTIPKIRKNLRHAIEMLNVKKKEFLSLIPNVTCNYIKT
                     KLEELDNTLGKWEPFLNDLKAVSPILDNHLDDIVKNMKPLIVQMIFEPSHYDDMFISR
                     KALTPVFSSVL"
     gene            complement(735..1940)
                     /locus_tag="POB92_002744"
     CDS             complement(735..1940)
                     /locus_tag="POB92_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6580167.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
BASE COUNT          647 a          276 c          315 g          795 t
ORIGIN      
        1 aaattttcaa aaatatagat cacgacttca gagatggagt atctgaagaa atgatgaaac
       61 atttgaaagt agtgaaacag aatatagagc aaataaaaaa tcaaaatgat atttatggta
      121 ggcaaattgc agatattaga agtattatga agcaacaaga tgcaacaatt ttagatggaa
      181 attatcaaat taattatagc ggtgaaaata tggtgcaggg tctagcttta ccttctaatt
      241 atttaggaag aaaaatgaaa atattaaaag accatatcga tgatggtatt aaaaaaatag
      301 cagactatgt tcaaggtata tatgatgaat atgcatcgaa aattgtcgat gtaataaaat
      361 atttgattaa tacaattccc aaaatacgta agaatttaag acatgcaatt gaaatgttaa
      421 atgtaaaaaa gaaagaattt ttgtccctga ttcctaatgt aacttgtaat tatattaaaa
      481 ctaaattaga agaattagat aatactttag gcaaatggga gccttttctt aatgatttaa
      541 aagcagtgtc accaatttta gataaccatt tagatgatat tgttaagaac atgaagcctt
      601 tgattgtaca aatgatattt gaaccatcac attatgatga tatgtttatt tcaagaaaag
      661 ctttaacgcc agtgttctca agcgttttat aaagcttgta aaaaatataa gggcaaaaaa
      721 agggcggatt taagctaact tggaatgttt tcgagttttt gagttagttc tctatccatt
      781 ttttcagtta catgagtata tatgcgaatg gttgtttttt catctacatg tcctaccctt
      841 ttcataattg cttttaaaga aacattcatt tctactaata aagttatgtg tgtatgtcta
      901 aatgtgtgcg tggtaacttt cttattcata tttaaagctt ttgtagtttt cttaagcaca
      961 ccggcgattt gattattaca taaaggattc cctttttttg ttgtgaatat gaaccctctg
     1021 tcaacatagc tcgaattcca tcttttcaac attttgtttt ccagtattat ctttttaaaa
     1081 atttctacgg ttctagaatt gatgctgata cttctttttg aacttatagt ctttgtagtg
     1141 tctttgtatc cgaatccttc ctcgtattta atgcggtgaa ttgtacctgt tatattgata
     1201 gttttgttta ataaatctat atctttttcc tgcagtgctt gtagttctcc tatgcgcata
     1261 ccagttaaag cctgtacttc taagatgctg gcaattaaaa tgcgatttcg cttgtgtaac
     1321 ttattatcat ttagtatatg atcacgtatc tgtaggactt ggttcatttc taaatagttg
     1381 tacattttag attcatcttt ttcgatatcc tctattgttt ttcttctttt aggaattttg
     1441 acattagtta acaaatattc atttggataa ttgtaaaatt taactgcata tttaatagct
     1501 cctttcatat ctccgagttg acgggttact tgattttgag aatagatatc tgataattta
     1561 ttaataaata tctgcatata ttttgtatct agtttgttta aaagcaagtt ctcagagctg
     1621 tatcgtttaa tgtttctaat tcttattttt atattattaa gagtagtcaa ctttgaacct
     1681 gatgttttta tatgatattc aagccattca tctaatagcg cgtgaaaagt caaagttttt
     1741 aattcgcttg acgacttgtt gttcagtttt tcttttattt tttcttctaa acgaaacatt
     1801 gcttcttttt gagattgttt tgtattcttg ttcaacacaa cacttacgcg cttccattta
     1861 tctgtgtatg gatctttata cttctcgtag tatctgtatt tagtttcgtt atttttgttt
     1921 ttaaattttt caatccacat gtttatacct cctgcaagaa cgtatgttct ataaaatatt
     1981 aaaaaataat aagggtagtc gggctacccg taatttagta ctaggtacta aat
//
LOCUS       DAMZLE010000245         2020 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMZLE010000245 DAMZLE010000000
VERSION     DAMZLE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.251"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            15..1481
                     /locus_tag="POB92_002745"
     CDS             15..1481
                     /locus_tag="POB92_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6580168.1"
                     /translation="MMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDE
                     IIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKG
                     ISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCY
                     QVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIM
                     ICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIV
                     LTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEV
                     AELVVKKDNSVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVV
                     STSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNIKDMADKILQ
                     LVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1570..1926)
                     /locus_tag="POB92_002746"
     CDS             complement(1570..1926)
                     /locus_tag="POB92_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6580169.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          824 a          236 c          326 g          634 t
ORIGIN      
        1 tgaaaaaaat atttatgatg gtacatgagt tagatgtcaa taaaggtggt atgacctctt
       61 cgatgttcaa tagaagtaaa gagttttatg atgcggacat acctgctgat attgttactt
      121 tcgattacaa aggaaactat gatgaaatta ttaaagcttt gaaaaaacaa ggtaaaatgg
      181 atcgaagaac gaaaatgtat aatgtatttg aatattttaa acaaatttca aataataaac
      241 attttaagtc taataaattg ttatataaac atatttcaga aagactaaaa aatacgattg
      301 aaattgaaga gagtaaaggt atttcaagat attttgatat aacgactggt acatatattg
      361 cctacattag aaaaagtaaa tctgaaaaag tgattgattt ctttaaagat aataaacgaa
      421 ttgaacggtt tagttttata gataataaag tgcatatgaa ggaaacattt aatgtagata
      481 ataaagtttg ttatcaagta ttttatgatg aaaagggata cccatatatt tcaaggaata
      541 ttaatgctaa taatggtgct gtaggtaaaa cttatgtgtt agttaataaa aaagaattta
      601 aaaacaattt agcactgtgt gtttactatt tagaaaaact aataaaagat tctaaagata
      661 gtattatgat ttgtgatgga ccagggagtt ttccaaaaat gtttaataca aatcataaaa
      721 atgctcagaa atatggcgtt attcatgtta atcatcatga aaatttcgat gatacgggtg
      781 catttaaaaa aagtgagaaa tatattattg agaatgcgaa taaaattaac ggtgtaattg
      841 tattaacaga ggcacaaaga ttagatattc ttaatcagtt tgatgtagaa aatattttca
      901 ctattagcaa ttttgttaag atacataatg ctccaaaaca ttttcaaact gaaaaaatcg
      961 taggtcatat ttctagaatg gtaccaacga agcgaattga tttgcttatt gaagtggctg
     1021 agttagtcgt aaaaaaagat aattctgtta aatttcatat atatggagaa ggatctgtca
     1081 aagataaaat agctaaaatg attgaagata aaaatttaga aagaaatgtt tttcttaaag
     1141 gatatacaac aactccacaa aaatgcttgg aagattttaa attagtcgtt tctacatctc
     1201 aatatgaagg tcaagggtta agtatgatag aagcaatgat ttctaaaagg cctgttgttg
     1261 cctttgacat caaatacgga ccaagtgatt ttatagaaga taataaaaat ggttatttaa
     1321 tagaaaacca taatattaag gacatggctg ataaaatact tcagcttgtt aataatgatg
     1381 tattagcagc ggagtttggt tcgaaagcga gagaaaacat tatagaaaaa tattcaacgg
     1441 aatcaatatt agaaaaatgg ttaaatcttt tcaatagcta aatgaaaaaa tcctaggtat
     1501 tcatgagtac ctaggatttt tacgcatgat atatacaaaa aaagagagga tagtcctctc
     1561 aaataatagt taataaaatt gtagccattt cttccagctg taactactaa tacaagctaa
     1621 gttcattacg atatgtatga tttcaaattc ataaccactt acatatcctc ttgtaagttt
     1681 aagtgtgaag attgagccta acataacaca aatataaaaa acattaacat aaggcgttaa
     1741 aaagccgata ataagtgcta aacctccaac aacttcacca attgacatca atactgcgat
     1801 ggtaggtggt aaacctaaag agcctaagaa gtgaattgtt ccatctaaat caacaaattt
     1861 caaagtacca tgcactaaga atgatgttgc taacatccat ctaattaaaa gcatacctaa
     1921 tttcatcatg tcacctccaa catacgtatt tttaaaacat agtccaaatt atatttgtaa
     1981 agatgtggaa agtaaagatt atttataaaa ataattatta
//
LOCUS       DAMZLE010000246         2014 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMZLE010000246 DAMZLE010000000
VERSION     DAMZLE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.252"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            321..1280
                     /gene="hyl"
                     /locus_tag="POB92_002747"
     CDS             321..1280
                     /gene="hyl"
                     /locus_tag="POB92_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDI6580170.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
     gene            1757..1990
                     /locus_tag="POB92_002748"
     CDS             1757..1990
                     /locus_tag="POB92_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580171.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          775 a          263 c          309 g          667 t
ORIGIN      
        1 atttttaaat caaacaaatc attagtttta aagaaattaa tcaatagaat tagctatgtc
       61 ttttccttgt ttcataaaga atttattcaa ctttgactaa ccctcgaaat tgaaatgctt
      121 cctttcaaat tttaaataaa agttaaaaac atattcttaa aataatcact catcatcact
      181 cagtaattta tcagttgcta tattaaatat actaatataa acgattataa atatttgata
      241 tgtctcaact gcaatattct aaattgacat attgattatt gtttcctcaa atcgtttaaa
      301 aaaatagaag gatgatgaaa atgaaaacac gtatagtcag ctcagtaaca acaacactat
      361 tgctaggttc catattaatg aatcctgtcg ctaatgccgc agattctgat attaatatta
      421 aaaccggtac tacagatatt ggaagcaata ctacagtaaa aacaggtgat ttagtcactt
      481 atgataaaga aaatggcatg cacaaaaaag tattttatag ttttatcgat gataaaaatc
      541 acaataaaaa actgctagtt attagaacga aaggtaccat tgctggtcaa tatagagttt
      601 atagcgaaga aggtgctaac aaaagtggtt tagcctggcc ttcagccttt aaggtacagt
      661 tgcaactacc tgataatgaa gtagctcaaa tatctgatta ctatccaaga aattcgattg
      721 atacaaaaga gtatatgagt actttaactt atggattcaa cggtaatgtt actggtgatg
      781 atacaggaaa aattggcggc cttattggtg caaatgtttc gattggtcat acactgaaat
      841 atgttcaacc tgatttcaaa acaattttag agagcccaac tgataaaaaa gtaggctgga
      901 aagtgatatt taacaatatg gtgaatcaaa attggggacc atatgataga gattcttgga
      961 acccggtata tggcaatcaa cttttcatga aaactagaaa tggttctatg aaagcagcag
     1021 ataacttcct tgatcctaac aaagcaagtt ctctattatc ttcagggttt tcaccagact
     1081 tcgctacagt tattactatg gatagaaaag catccaaaca acaaacaaat atagatgtaa
     1141 tatacgaacg agttcgtgat gattaccaat tgcattggac ttcaacaaat tggaaaggta
     1201 ccaatactaa agataaatgg acagatcgtt cttcagaaag atataaaatc gattgggaaa
     1261 aagaagaaat gacaaattaa tgtaaattat ttgtacatgt acaaataaat ataatttata
     1321 actttagccg ataacttcag aaatgatgtt tttcggctaa tttttatact taatttagtt
     1381 taattaactt atgatatttt taatttaaac tatgtacttg atttgctttc ctgacttaaa
     1441 aattagacta aacatgacac tatatgaggt gatttttaaa ataagaaatt tgaattccat
     1501 gttgtcaaac cctttatata aaagttttga aattgttcaa aactttttga acacattatg
     1561 tgttaatata tttgcagaat tattttaaaa tcgttttaaa actatactga aataagaaat
     1621 ttaaaagtat aattagtaca tataattgaa aaagatttct agaaatacaa ttaatgttca
     1681 tctagatatt gtacgaagtt gtatgaataa gttaaactta agttgaatca atttaaatgt
     1741 tcaaggaggt taacaaatgg tttttaatga ttgggaaatt aacgttgtat actcaggaga
     1801 gcatgaggtt gtaacaaatg aaaagagttt atttgtcatt gacgaatttt acgatgttgc
     1861 aatcgcaatc tattatttag atggaaaatt aaaagtagct catgtcaact atggttctga
     1921 ttttacaatt gacgtagcta ataaagtttt tgaattgaat attcaaaatc caaatgttga
     1981 tgagcattaa atattaaaaa gcatttagaa tcat
//
LOCUS       DAMZLE010000247         1935 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMZLE010000247 DAMZLE010000000
VERSION     DAMZLE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.253"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            50..1072
                     /gene="emp"
                     /locus_tag="POB92_002749"
     CDS             50..1072
                     /gene="emp"
                     /locus_tag="POB92_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDI6580172.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPTYNEFKKAPKVNVGNLADNKNFVASEDKLNKIVDSSAASKIVDKNFAVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            1407..1928
                     /locus_tag="POB92_002750"
     CDS             1407..1928
                     /locus_tag="POB92_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580173.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVSESVETNFVVKDSDNK
                     NILQTHTEITTEEKFSVVEKSQLNTLKSLSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKYTQKYEDLAKATAKVSTLSQSHQNYLNKQI
                     DKVNNKIEKTEKR"
BASE COUNT          787 a          295 c          283 g          570 t
ORIGIN      
        1 gaaattatat tggttataat attgagtcta tataaaggag aaataacaga tgaaaaagaa
       61 attattagtt ttaactatga gcacgctatt tgctacacaa attatgaatt caaatcacgc
      121 taaagcatca gtgacagaga gtgttgacaa aaaatttgta gttccagaat caggaattaa
      181 taaaattatt ccaacttaca atgaatttaa aaaagcacca aaagtaaatg ttggtaattt
      241 agctgacaat aaaaactttg tagcttctga agataaattg aataagattg tagattcatc
      301 ggcagctagt aaaattgtag ataaaaactt tgccgtacca gagtcaaagt taggaaacat
      361 tgtaccagag tacaaagaaa tcaataatcg cgtgaatgta gcaacaaaca atccagcttc
      421 acaacaagtt gataagcatt ttgttgctaa aggcccagaa gtaaatagat ttattacgca
      481 aaacaaagta aaccaccact tcattactac gcaaacccac tacaagaaag ttattacttc
      541 atacaaatca acacatgtac ataaacatgt aaatcatgca aaggattcta ttaataaaca
      601 ctttattgtt aaaccatcag aatcgcctag atatacacat ccatctcaat ctttaattat
      661 caagcatcat tttgcagttc ctggatatca cgcgcataaa tttgttacac cagggcatgc
      721 tagcattaaa attaatcact tttgtgttgt gccacaaata aatagtttca aggtaattcc
      781 accatatggt cacaattcac atcgtatgca tgtaccaagt ttccaaaata acacaacagc
      841 aacacatcaa aatgctaaag taaataaagc atatgactat aaatacttct attcttataa
      901 agtagttaaa ggtgtgaaga aatatttctc attttcacaa tcaaatggtt ataaaattgg
      961 gaaaccatca ttaaatatca aaaatgtaaa ttatcaatat gctgttccaa gttatagccc
     1021 tacacactac gttcctgaat ttaagggtag cttaccagca ccacgagtat aaaaattggc
     1081 actaagttta cgagatatga taaataccta ttattttaaa tatagtctac aatctatgtg
     1141 gttgtaggct gtattttttg cagtttatca ataaacaccc atcaacaaat tataccgttt
     1201 ttctactttg aaagttggaa gtaacataat cttaaataaa tatattatta attaagataa
     1261 atataatact caagattatt gttaatagtt tgttcatcgc aagttaatta ttgtttctaa
     1321 aatattggta tataattttc aatggcgaag aaaacagggt gaaaaagtcg gtttttaaat
     1381 caaagcaaat aagggagcat aaacaaatga aaaggaaagt attagtacta acaatgggtg
     1441 taatttgtgc aactcaatta tggcattcta atcacgcaaa cgcattagta tcagagagtg
     1501 ttgaaactaa ttttgttgta aaagattcgg ataataaaaa tattttacaa acccatactg
     1561 aaattactac tgaggagaaa ttttcagtag tagaaaagag tcaattaaat acactaaaat
     1621 cactatctaa tgacaactac atagaatatg atttacatac taatcaaaca ggcataaaaa
     1681 aaggttggtt atatggttat agtgaaattg actcatcaca tttcacagac cgtgacaaac
     1741 gcgttattag acgtgatcat gttaaagaag cacaaagctt aattaatgat tataaatata
     1801 cgcaaaaata tgaagatctc gctaaggcaa ctgcaaaagt aagtacactt agtcagtctc
     1861 accaaaatta tttgaataaa caaattgata aagtgaataa taagatagag aaaactgaaa
     1921 aacgctaatc caaag
//
LOCUS       DAMZLE010000248         1921 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMZLE010000248 DAMZLE010000000
VERSION     DAMZLE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.254"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            238..1647
                     /locus_tag="POB92_002751"
     CDS             238..1647
                     /locus_tag="POB92_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6580174.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDVL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1651..>1921
                     /locus_tag="POB92_002752"
     CDS             1651..>1921
                     /locus_tag="POB92_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580175.1"
                     /translation="MLFNVKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHT"
BASE COUNT          724 a          238 c          375 g          584 t
ORIGIN      
        1 ttaaaatatt gaaagataat ttttattgat taattgcgaa tatacaaaga caccattata
       61 tccatatgct aaaataagaa aaaaggcgac atgttatctc aatttacctg catctagact
      121 cctttacata agaaataatc atcgccgagt tttaaaattt tacaactttg tagatgtaaa
      181 aagcatttga ttttaaatag tttgaaaatc atatattttt gaaaaaggag catgccgatg
      241 agtattgaca tgtatttaga cagatctcga aaccaagctt caagtgtggg gaatttgagt
      301 caaacaatga attcaaatta tgatgcgttg gaaaaagcaa ttactcaatt tatcaatgat
      361 gatgtgctta aagggaaagc gtatacgtca gctaagcaat tttttagtac ggtgttaatt
      421 ccattatcaa caagtatgaa aacattgagt gatttaacga agcaagcttg cgataatttt
      481 gtgtcacgtt atacgagtga ggttgatagc atatctttaa aagaatcaga gcttgaagaa
      541 gatatcagat cattaagtca acaaattacg cgatatgaaa atttgaataa caatttgaaa
      601 aagcatgctt ccgataatca gcaagccatt tcatcgaacc aacaaataat acaaacatta
      661 ggtcaacaaa aacatgaatt agaagagaag ctacgcaaat tgcgtgagtt taatcaaaaa
      721 tcaccagaaa tatttaaaga agttgaagaa tttcaaaaaa ttgtccaaca aggacttacc
      781 caagcgcaaa atttttggaa cttttcaaca aatcaattta atattccttc aggtaaagaa
      841 cttgattggg ccaaagcaag tcatgaaaaa tatttgaaag ttgctatggg gaaaattgaa
      901 cataaagcag agaaagaaac tttaaataaa gcagactttg ctgttataaa ggcatatgcc
      961 aaagaacatc cagaagacga tatcccgaaa agtatattga aatatataaa tgacaataaa
     1021 gacagtatta aaagagatat aggattagat attacgtcaa cacttttaga gcaaggcggt
     1081 ataaatgcaa gtaaattcgg tgtatttatc aatacagcag gtggagtgaa aggcccagca
     1141 ggtccaaatt catttgtgga agtcaaacgt acatcaggca atgtgtttat agaaaatggt
     1201 agtaaatttg caaaaggcgg aaaataccta ggtaaaggtg ttgctggtgt aggatttggt
     1261 ataggtatgt atgatgacct tgcaaatgat gataaaacat ttggagaggc gttgtcgcat
     1321 aatggtatga cgcttgcagc tggatctgca gggacagcag ttggagctgg attagcgact
     1381 tttgttttag gaagtaatcc agtaggatgg gtgattttag caggtttggc tatgagtaca
     1441 gtatttgcat taggaacaga tttaatttat caaaataata tttttggatt aaaagataaa
     1501 gtagactggg tagggcataa aatcgataat agtatagatg ttgttaaaaa aactacagaa
     1561 aaatccatgg atagtgttgg gaatgctgtt agtgaagcta aaaatattat tagcaatcat
     1621 ataaatccaa tgaaatgggc gtggtgagtt ttgcttttta atgttaaggt tattaataaa
     1681 aatccgagat ataaagtcgt tcaatataat gatgagtatt tgttaattga tttagtaagt
     1741 acttggcttg tatacttttt tccttttatt aattggttca ttcccaaaag gtatgcaaaa
     1801 cttagtgaga aggaacttga aaatttaaat gttgataaac aaaataaaaa taatattttc
     1861 tggccagtta ctggtagttc gtttttattt gtagttattt tacggaaata tgtacatacg
     1921 t
//
LOCUS       DAMZLE010000249         1911 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMZLE010000249 DAMZLE010000000
VERSION     DAMZLE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.255"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            98..811
                     /locus_tag="POB92_002753"
     CDS             98..811
                     /locus_tag="POB92_002753"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6580176.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            complement(1251..1886)
                     /gene="sdpA"
                     /locus_tag="POB92_002754"
     CDS             complement(1251..1886)
                     /gene="sdpA"
                     /locus_tag="POB92_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDI6580177.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGIIYIKTKRLEVVIFMHFLNNLLALLFIIWG"
BASE COUNT          748 a          252 c          315 g          596 t
ORIGIN      
        1 taacaaagaa ataaaaatca ttttttacaa atggttgtaa gaaaaagaca tgcagatgtt
       61 gttaaaattt taataagaat caaggaggca atattatatg gctaaactaa atgtagaagt
      121 atttgcggac ggtgcagata ttgaagaaat gaaagcagct tataaaaaca aacaagtgga
      181 tggttttaca acaaatccta gtttaatggc gaaagcgggc gtaacagatt acaaagcttt
      241 tgctgaagaa gctgtgaaag aaattccaga tgcttcaatt tcatttgaag tatttgcaga
      301 cgatttagaa actatggaaa aagaagcagc aattctaaaa caatatggcg aaaatgtatt
      361 tgttaaaatt cctattgtaa atacaaaagg tgaatcaacg attcctttaa ttaaaaagct
      421 ttcagctgac aatgtgagat taaacgttac ggctgtttac acaattgaac aagttaaaga
      481 aataactgaa gcagtaactg aaggtgtgcc aacatatgtt tcagtatttg caggacgtat
      541 tgcagataca ggcgtagatc cattaccatt aatgaaagag gctgtaaaag ttacgcatag
      601 taaagacggc gttaaattat tatgggcaag ttgccgcgaa ttattcaatg tgattcaagc
      661 tgatgaaatt ggtgcagata ttattacatg ccctgcagat gttgtgaaaa aagtgaatac
      721 aaacttaggt cgcgatatta acgaattatc agtagataca gttaaaggct ttgcgaaaga
      781 tattcaatct tcaggtcttt ctattctata atatataaat atatttaaac acttataggc
      841 attgtgctta tgggggtttt tccataatag ttattaatga agaagaagtc aaaattcagc
      901 taagtaaaag ctcgagttat aaaaaagtct aaattgattc aacaggagta gaaaaggaaa
      961 ttagtctcgc atattttaga aaatgaataa aaacaattac tatatgcaac ttaagaatta
     1021 aatattattt aagccacata cctattagag tacaaaatat acaagttgta cttatatcta
     1081 ggtatgtgac ttttgatttt aagcgtaaag caaaagtttt tatgtttgta ttagttctaa
     1141 attattttgc acttgatgac acgtattttt gagtttaagt ggtgactcaa gttcaataaa
     1201 ataaatatca gatagataat ttgaaaataa tatgaaaggt tatctcaaaa ttatccccat
     1261 attataaaga gcagagctaa caagttattt aagaagtgca tgaatattac cacttctaat
     1321 cgttttgttt ttatataaat tataccaaaa atcaaaccag aatataagta aggtaaataa
     1381 ccaatccaag tgtcagattc gtgtaatgat gcaaaaacta aactagacac aattaaccct
     1441 aaaaacaagt gttttctgaa gataaccctt attatcatac cgcgaaaaac aatttcttcc
     1501 acaatagcgg ggatgatggt aacagtgaaa atagaaatat agaatggtgt atttcgtatt
     1561 tcttgctcta attgttgttc atttgccggt acgtcatgaa aatataaata aagattatct
     1621 aacccataaa ttaatagtgc accgcctata atataaataa cttgagacaa tgttaatttt
     1681 ttaaaagaga ataaattgat tttgaatagc ctcaatagaa ttaaacctac taatacaatt
     1741 agcatgctat aaagtaaatg tacttcactg acaactatgc cgataaataa aatgatagta
     1801 ataaaaaatg ctgccattaa agttaaaaaa taatttcctc gtgtaaccga taaatcatcg
     1861 taaaaaaagt ctttgaattt ttgcatgcta aatcctttct tttcattttt t
//
LOCUS       DAMZLE010000250         1904 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMZLE010000250 DAMZLE010000000
VERSION     DAMZLE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.256"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(363..698)
                     /locus_tag="POB92_002755"
                     /pseudo
     CDS             complement(363..698)
                     /locus_tag="POB92_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1349..1840)
                     /gene="sak"
                     /locus_tag="POB92_002756"
     CDS             complement(1349..1840)
                     /gene="sak"
                     /locus_tag="POB92_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HDI6580178.1"
                     /translation="MLKRSLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVI
                     EKK"
BASE COUNT          653 a          348 c          276 g          627 t
ORIGIN      
        1 aacaaaaaaa ggtaagcacc gaaatgctta cctacttccc ataaacaata taacacatat
       61 acattgattt ggaaagcgca aaaataaatt taatatacta gcccgaaggg gagcaataca
      121 taaaaaatga aaggcgctcc ttgaaaacgc ccaaggtaat attaacataa aatggctact
      181 attgcattat ctaattttat ttaattaaaa caaatatata gcacaaaaaa actagcccga
      241 aggctagctt atgaatagat gaaaatttga acacattgct gtgtctaaaa tgattatagc
      301 ataatttcaa actattctat aataaattaa ttaacttcat atgaaacttt acacactttt
      361 tttcaaagtg cgctaaaatt accaaaactg cttattctat tacctgcctt gtctacctct
      421 cctgtcgcta tataacaacg ttgtccatta ttagcaatat aagtaatcca tctatagcca
      481 ttaatgcaat atgcgccgtc atatttgatc gttgcgttat tgggtaatac acctgtaatt
      541 cttgaattag ttgaatagcc atccctcacg ttattacctt taacattggc aactgtgtaa
      601 taaccagtct cttttttata cggtacattg tttttatcga gtgtataacc tgctggcact
      661 ggtgaattct tttcattttt agctggtgtt ttaacattac tgatacctga tacacacttc
      721 caataaaaat aaccacacca tttaagatgc ggtgtagcga ctgtaatatt tctatgttgt
      781 tgagatatat gtatcgagtg aagggcaaaa aggatatcaa ttgcaggata agtgttaggt
      841 tactaggcca cttaacaggc tatatagttc actcctacta tatacactta attataacat
      901 aaaaagcacc ccgtaaacta ttatacggga atgctaaagt catatatact acgggggagt
      961 agtatgaaaa ctatgctctc tatcataaga aaaaaacacc cagtgacatg cttgggtgaa
     1021 caaggataga tgtaaatagt tgatgcatgt gtaacacatc atagcaaaaa actagcccga
     1081 aagctagcta taacatacaa tctaaaaaga cgtcctttga atacgtctag aaagattata
     1141 acataaaaaa ataggcaagt accgaagtac ctgcctaaaa aaggattatc cactttttca
     1201 tcctaactga tttctcccca taagtcacct aatatctgat taggtgggga agaaccattc
     1261 gtgcatgaat gagaatttga tgaaagataa ttttcactac acatattcaa tcaagacatt
     1321 gctttctata ataaacaact attttgtttt atttcttttc tataacaacc tttgtaatta
     1381 agttgaatcc agggttttta atatgctctg ataaatctgg gacaacaaaa cctttttctg
     1441 ttatagggaa agacttcgtt tcttcttttt tcttattctt atcataataa gtgacttcga
     1501 tctttgcgct tggatctaat tcaactactc taaactcttt atatgctgtc gcatctaatg
     1561 cccattcgac atagtattca attttttctt ttgtaagtgt agtcccaggt ttaataggaa
     1621 actcgacata atgaggggat agcaattcat ttcctttacc atcaactcca gtcacattta
     1681 ccatcaaata cgggcctgtt ggttcaaaat aactcgcgtc atcgcctttt ttatattttc
     1741 ctttgtcgaa tgaacttgat gcacttacct cattagtaat tgaagaaaat gagaataata
     1801 acaataaaac agttaaaaat aataaacttc ttttgagcat ggcgcttcct ccaaatataa
     1861 tatatttgtt tttatttatc tctttttata gctaacaccc ttat
//
LOCUS       DAMZLE010000251         1893 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMZLE010000251 DAMZLE010000000
VERSION     DAMZLE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.257"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            166..891
                     /gene="sasD"
                     /locus_tag="POB92_002757"
     CDS             166..891
                     /gene="sasD"
                     /locus_tag="POB92_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDI6580179.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALSQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGINHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1082..1837)
                     /locus_tag="POB92_002758"
     CDS             complement(1082..1837)
                     /locus_tag="POB92_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6580180.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
BASE COUNT          665 a          311 c          321 g          596 t
ORIGIN      
        1 aatcaacata ttgacattaa ataaattgac aaaataagta attattgtga atctaaagtg
       61 aaatttttat aaaaaaatgt aatgattcaa aaattttgtt gcatttcttt tgtaatcgta
      121 tgataatgta aatgtaatca aattgtaata taaggggaca agacaatgaa aaaattagca
      181 acagtaggtt ctttaattgt aacaagcact ttagtattct caagtatgcc ttttcaaaat
      241 gcgcatgccg acacaacttc aatgaatgtg tcgaataaac aaagccaaaa tgtacaaaat
      301 catcgtcctt atggcggagt agtaccacaa ggaatgacgc aagcacaata tactgaatta
      361 gagaaagctt tatcccaatt aagcgctggc agtaatatgc aagactataa tatgaaattg
      421 tatgatgcga cgcaaaatat tgctgataaa tacaatgtga taattacaac taatgtaggg
      481 gtatttaaac cacatgctgt tagagatatg aatggccatg cgttaccttt aacaaaagat
      541 ggcaattttt atcaaacgaa tgtagatgca aatggtatta atcatggtgg tagtgaaatg
      601 gtgcaaaata aaacaggtca tatgagtcaa caaggccata tgaatcagaa cacacacatg
      661 aaccaacagc cacacatgca acaaggtcat atgcaatcat caaaccatca aatgatgagt
      721 ccaaaagcaa atatgcattc atcaaatcat caaatgaacc aaagtaacaa aaaagtttta
      781 ccagctgctg gtgaaagtat gacatcaagt attcttactg caagtattgc cgcactacta
      841 ttagtatctg ggttattctt agcatttaga cgacgttcaa caaataaata aacataatac
      901 gattaataat agaaaaatcg tgtgattatc tgagcgagcc taggacataa atcaatgtcc
      961 taggctcgct aatgttatat tggcagtagt tgactgaatg aaattgcgct tgtaacaagc
     1021 ttttccattt cttaccaact ccctaaatag tcatcaaaaa atttcttata ttttaataat
     1081 tttaataatc cgattgtctt atacgtgtca gtgttaattc agagatttcc tgtggaatat
     1141 accacttatt aatcataatt ggataaggtg tttgtgcgta caatgtttca ataatcagcc
     1201 aacaatgtgt atcaccatca aacacgtgac tatgattttt gaagtggggc gctttggtaa
     1261 tagacatttt taaatctgat tgatatgcat tgctataaat cgtttgctca acgaatgtct
     1321 tcatgtcgtc ttcgttttgt gtattcactt taaatgtgtc aatgacattt aacggtataa
     1381 aggtaaagca aaatgcatca gcttgcttag aatgattgtc ctttttttga taatagcgtt
     1441 ccattgcaat gacggcagaa ggatggtttg caaacaaatg atttgtatat tcactttcta
     1501 aatcaacacg ataattaatt gatgacatag atacgcgagc tagcaatatt tgatcaagtg
     1561 gatgcttaaa ttgatccata cttgaagcgt gttgggcatt tgtttgtgga ataacaaagt
     1621 gtcccttccc tcttgtactc tctacgatgc catcttcggc taacaatttt atagcttggc
     1681 gcaaagtcat acgactgaca tcaaagcgcg cacaaagttc cttttcagta ggtaatgcat
     1741 ggccactcgg atattttcct atttgaattt ctttatataa cgtattataa atcgttaaaa
     1801 attttggttg tgtttgcgtc acgtagacaa cctccataaa gttacttaat cactctcatc
     1861 atacaataat ttttactcga attggaaaaa tta
//
LOCUS       DAMZLE010000252         1858 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMZLE010000252 DAMZLE010000000
VERSION     DAMZLE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.258"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            62..622
                     /locus_tag="POB92_002759"
     CDS             62..622
                     /locus_tag="POB92_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6580181.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            675..1604
                     /locus_tag="POB92_002760"
     CDS             675..1604
                     /locus_tag="POB92_002760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDI6580182.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
BASE COUNT          623 a          289 c          316 g          630 t
ORIGIN      
        1 ttactctagc atttatgaat agatgggagt ttattttatt attatatagg agagatgttg
       61 aatgacacat cgcgcactat tagttgttga ctattcatat gactttatcg cagatgacgg
      121 cttactaaca tgcggtaaac ctggacaaaa tattgaagat tttattgttt ctcgtatcaa
      181 tgactttaat tattatcaag accatatatt ctttttaatg gatttacatt atttacatga
      241 cattcatcat cctgaaagta aattattccc accacacaat atcgtagata caagtggtag
      301 agaattatac ggtaaagtag gtaaattata cgaaacaatt aaagcgcaac ctaatgtaca
      361 tttcattgat aaaacgcgct atgattcgtt ctttggtacc ccgcttgata gtttattgag
      421 agaaagaagt attaatcaag tcgaaatcgt tggtgtatgt accgatattt gcgtgttaca
      481 tacagcaatt tctgcataca acttaggtta taaaatttca gtacctgctg agggagtggc
      541 ttcatttaat caaaaagggc atgaatgggc acttgcacat ttcaaaaact cattaggtgc
      601 agaggtagaa caacacgttt aaatcgtgct aaaataatta taaagaatac aatttacaag
      661 ggagatattt gacaatggct aaaacatata ttttcggaca taagaatcca gacactgatg
      721 caatttcatc tgcgattatt atggcagaat ttgaacaact tagaggtaat tcaggagcca
      781 aagcataccg tttaggtgat gtgagtgcag aaactcaatt cgcgttagat acatttaatg
      841 tacctgctcc ggaattatta acggatgatt tagatggtca agatgttatc ttagttgatc
      901 ataacgaatt ccaacaaagt tctgatacga ttgcctctgc tacaattaag catgtaattg
      961 atcatcacag aattgcaaat ttcgaaactg ctggtccttt atgttatcgt gctgaaccag
     1021 ttggttgtac agctacaatt ttatacaaaa tgtttagaga acgtggcttt gaaattaaac
     1081 ctgaaattgc cggtttaatg ttatcagcaa ttatctcaga tagcttactt ttcaaatcac
     1141 caacatgtac acaacaagat gttaaagcag ctgaagaatt aaaagatatt gctaaagttg
     1201 atattcaaaa gtacggctta gatatgttaa aagcaggtgc ttcaacaact gataaatcag
     1261 ttgaattctt attaaacatg gatgctaaat catttactat gggtgactat gtgactcgta
     1321 ttgcacaagt taatgctgtt gaccttgacg aagtgttaaa tcgtaaagaa gatttagaaa
     1381 aagaaatgtt agctgtaagt gcacaagaaa aatatgactt atttgtactt gttgttactg
     1441 acatcattaa tagtgattct aaaattttag ttgtaggtgc tgaaaaagat aaagttggcg
     1501 aagcattcaa tgttcaatta gaagatgaca tggccttctt atctggtgtc gtttctcgaa
     1561 aaaaacaaat cgtacctcaa atcactgaag cattaacaaa ataatactat attactgtct
     1621 aattatagac atgttgtatt taactaacag ttcattaaag tagaatttat ttcactttca
     1681 atgaactgtt ttttatttac gtttgactat tttacaaccc cttttcaata gtagttttta
     1741 ttcctttagc taccctaacc cacagattag tgatttctat acaattcccc ttttgtcttt
     1801 acattttctt aaaatatttg cgatgttgag tataaatttt tgttttcttc ctaccttt
//
LOCUS       DAMZLE010000253         1850 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMZLE010000253 DAMZLE010000000
VERSION     DAMZLE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.259"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..1597
                     /gene="vwb"
                     /locus_tag="POB92_002761"
     CDS             71..1597
                     /gene="vwb"
                     /locus_tag="POB92_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HDI6580183.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
BASE COUNT          809 a          223 c          317 g          501 t
ORIGIN      
        1 aatttggctt ggcgaaaaaa tagggtgtaa ggtaggttgt taattaggga aaattaagga
       61 gaaaatacag ttgaaaaata aattgctagt tttatcattg ggagcattat gtgtatcaca
      121 aatttgggaa agtaatcgtg cgagtgcagt ggtttctggg gagaagaatc catatgtatc
      181 tgagtcgttg aaactgacta ataataaaaa taaatctaga acagtagaag agtataagaa
      241 aagcttggat gatttaatat ggtcctttcc aaacttagat aatgaaagat ttgataatcc
      301 tgaatataaa gaagctatga aaaaatatca acagagattt atggctgaag atgaggcttt
      361 gaagaaattt tttagtgaag agaaaaaaat aaaaaatgga aatactgata atttagatta
      421 tctaggatta tctcatgaaa gatatgaaag tgtatttaat actttgaaaa aacaaagtga
      481 ggagttctta aaagaaattg aagatataaa aaaagataac cctgaattga aagactttaa
      541 tgaagaggag caattaaagt gcgacttaga attaaacaaa ttagaaaatc agatattaat
      601 gttaggtaaa acattttatc aaaactatag agatgatgtt gaaagtttat atagtaagtt
      661 agatttaatt atgggatata aagatgaaga aagagcaaat aaaaaagcag ttaacaaaag
      721 gatgttagaa aataaaaaag aagacttaga aaccataatt gatgaatttt ttagtgatat
      781 agataaaaca agacctaata atattcctgt tttagaagat gaaaaacaag aagagaaaaa
      841 tcataaaaat atggctcaat taaaatctga cactgaagca gcaaaaagtg atgaatcaaa
      901 aagaagcaag agaagtaaaa gaagtttaaa tactcaaaat cacaaacctg catctcaaga
      961 agtttctgaa caacaaaaag ctgaatatga taaaagagca gaagaaagaa aagcgagatt
     1021 tttggataat caaaaaatta agaaaacacc tgtagtgtca ttagaatatg attttgagca
     1081 taaacaacgt attgacaacg aaaacgacaa gaaacttgtg gtttctgcac caacaaagaa
     1141 accaacatca ccgactacat atactgaaac aacgacacag gtaccaatgc ctacagttga
     1201 gcgtcaaact cagcaacaaa ttatttataa tgcaccaaaa caattggctg gattaaatgg
     1261 tgaaagtcat gatttcacaa caacgcatca atcaccaaca acttcaaatc acacgcataa
     1321 taatgttgtt gaatttgaag aaacgtctgc tttacctggt agaaaatcag gatcactggt
     1381 tggtataagt caaattgatt cttctcatct aactgaacgt gagaagcgtg taattaagcg
     1441 tgaacacgtt agagaagctc aaaagttagt tgataattat aaagatacac atagttataa
     1501 agaccgatta aatgcacaac aaaaagtaaa tactttaagt gaaggtcatc aaaaacgttt
     1561 taataaacaa atcaataaag tatataatgg caaataatta atgcatggct gcaaagcaaa
     1621 taatgagttt gtcgtaaaaa taacaacatt ttaaactagc aataaataat atcaaagtca
     1681 tcatttcaat gatgcaatct agtatagtcc acattctaaa caggtgtgga ctattacttt
     1741 tttcacttta tattacgaaa aaattattat gcttaactat caatatcaat aattaatttt
     1801 aagctgaaaa acaataaaaa tgttaagaca acgtttactt caagttaatt
//
LOCUS       DAMZLE010000254         1724 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMZLE010000254 DAMZLE010000000
VERSION     DAMZLE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.260"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(116..1033)
                     /locus_tag="POB92_002762"
     CDS             complement(116..1033)
                     /locus_tag="POB92_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6580184.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKTKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(1033..1524)
                     /gene="lspA"
                     /locus_tag="POB92_002763"
     CDS             complement(1033..1524)
                     /gene="lspA"
                     /locus_tag="POB92_002763"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI6580185.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT          597 a          310 c          215 g          602 t
ORIGIN      
        1 cagtaaaaat gttaacctta atctatttta taatgtgttt tcattttaaa aaaacgaaaa
       61 cgataaatac ttatagttta cataataatg ttatcgtaat tatagcacaa cacaattatg
      121 catctctttt tcgaattgta tctaataaat cttcaaagtc ttgtggtaat tcagcatgtc
      181 tttcaatata ttcacctgtt actggatgtt cgaatccaat aagtccagca tgtagagctt
      241 gaccaccaat atccaatgtc tttttcggtc catactttgg atcaccaact aatgggaagc
      301 caatatattt catgtgcaca cggatttgat gcgtacgtcc tgtttcaagt tgacattcaa
      361 caagcgtata atctttaaaa tgttctagta cgttaaaatg tgtcactgct tccttaccat
      421 catcaacaac agccatagat tgacgatcat ttttgtttct accaattggc gcatcgattg
      481 taccgtaatc atgaggaata ttcccgtgaa ctaaagcgat atattttctt ttaacagatt
      541 tatccattaa ttgttctaca agcccacgat gagcaatatc atttttagca accattaata
      601 aaccagaagt atccatatct atacggtgaa caatacctgg acgaatttct ccattaatac
      661 ctgacaaatt tttaatttga tacattaaac cattaactaa tgtattggta taatgccctg
      721 gtgatggatg aactaccatg cctttcgttt tatatacaac tgcaacatcg tcatcttcat
      781 aataaatatc taaatttaaa ttttcaggta gaatatcagc ttcaaccact tctttttcag
      841 tgacaactat atgatcatta agtttcactt tataattaga tttaacaact ttatcgtttg
      901 caacgactaa acctgcttta atccaatctt gtatctggtt acgagaccaa tcactattta
      961 attcaggcag caacttatct acacgcatac ctgtttgttc tttatctgta atgttaaatt
     1021 cataagtctc cattacttaa cctccttctc ctttttattg gaagtatcct ttaataaggc
     1081 aataataatt aatattacac caattgttaa acttgaatct gcgatattaa atattggaaa
     1141 atcataacca aaaatatttg tatcaataaa gtcaacaact tctcctgtta aaattctatc
     1201 aataaagttt ccaagtgcac ctgcaaaaag taaactaata gcaacttgca taaacaaatt
     1261 atattgagca tctttaataa agaaatatac taaggctatt aatataataa tggtaataat
     1321 aaagaaaaat gtcatttttc cactcaatat tccccatgca gcaccattat ttcgatgtga
     1381 tgttatgttt aaaaagtgcg gtatcacttc aaatgaatct ccaattttca ttgtagtagc
     1441 tataatatat ttagtaactt ggtcaaatat aacgacaaat actgctatta aaatggaagt
     1501 gccaataaaa tattttttgt gcattttcgt tcctccaatc aatcgtccat gagacaactc
     1561 tttatattat agcttacacc tgctaataaa aaaagtaagc atattacatt aaatctaatg
     1621 ttactcactc aatacttgat aaactactat gttttgacat taaaaattag gaaagcaggc
     1681 tgggatatta agttcttagg caatttaaaa aagcagtttt ctat
//
LOCUS       DAMZLE010000255         1622 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMZLE010000255 DAMZLE010000000
VERSION     DAMZLE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.261"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(63..1544)
                     /gene="cls"
                     /locus_tag="POB92_002764"
     CDS             complement(63..1544)
                     /gene="cls"
                     /locus_tag="POB92_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6580186.1"
                     /translation="MRYTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGKVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLQDIKNAKEYIHLEY
                     YTFALDGLGQRILKALEEKLKQGLEVKILYDDVGSKKVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATYSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEMVSVGTANMDFRSFELNFEVNAFVYDEKLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          553 a          309 c          210 g          550 t
ORIGIN      
        1 cgttatttta attttgggca aaataaaaag agcctctata atcgagactc cttacaaata
       61 aattataaaa ttggcgaaac taatttagct aacgattctt taaacttaac agacagcggt
      121 ctattggcat atgattcttt ggttagttgt tttgattttg taatatcatg ttcataagcc
      181 acccttaaat ctttagcaag cttttcatca tatacaaaag cattgacttc gaaattcaat
      241 tcaaaacttc taaagtccat attagcagtt ccgactgaaa ccatttcatc atctattaaa
      301 cacattttag aatgtataaa tccattttca tacgtgtaaa tttttacgcc actagatagt
      361 aaatctgaag catttgaata tgtcgcccaa taaactaaag gatgatctgg cttacatgga
      421 atcattagat gaacatctac ccctgattta gctgcaattt taatggcatt tatatatgag
      481 ttatccggaa tgaaataagg cgattgcaaa tataccgact tcttagcgct cataatcatt
      541 tttgtataac catattcaat ttgatgccag tcactagccg gaccacttgc agctatttga
      601 attggtgaat tgcccaatgg tccgttcttt ttagggaaat acttaacatc atattcaaac
      661 tgtggacggt gcgcttgcga attccagtct aaaataaatc gcaactgcag tgcatcaacc
      721 gcatcccctt gtatacgtaa atgcgtatct ctccaatacc ctagtttacc taatcctaga
      781 tattcatcac caatgttaaa tcctccgaca taacctagtt gaccatcgat tacgatgatt
      841 tttctatgat ttctgttatt cattctgaaa ttcaataacg gtaatttaga agcaaaaaat
      901 gcttcaactt ctccacctaa cgatttaaaa tgatcaaaat tagccatctt aactttcttt
      961 gaaccaacgt catcatataa aattttcact tctagacctt gtttcaattt ctcttctaac
     1021 gcttttaaaa ttctttggcc caagccatct agagcgaatg tataatactc taaatggatg
     1081 tattctttag catttttaat atcttgtaaa acttgatcat ataaatcatt tccatcaatg
     1141 aaatgatcaa ctttattatt ttcagttaaa aaaccatctt gatccatcaa aagcatacgt
     1201 actaaatcat gatgtttttg aacttgtttg ttatcagtac cataattacc tttatcaaag
     1261 ctttctattt gttgttttaa aagtccatcg aaatccgtta acaccttacc attgttttta
     1321 ttcaatttac gtgccgaaac ggttctacca aaaaacaagt aaagaataaa accaatcaat
     1381 ggtaagacaa aaagtacaaa tagccatgcc caagttgaac tcgctgtacg tctatttctt
     1441 tctaaaaaga taataataaa agccaatact aaattaatga tgaatccaat ggctaaaata
     1501 atggtaaaaa gtgttcctaa atcatttgaa aatgtatatc gcatagtctt ttctcctata
     1561 aagaaaggca caaaaaacat tttgcacctt tcacatcata ttatttattc acagataaag
     1621 tt
//
LOCUS       DAMZLE010000256         1622 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMZLE010000256 DAMZLE010000000
VERSION     DAMZLE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.262"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            109..897
                     /locus_tag="POB92_002765"
     CDS             109..897
                     /locus_tag="POB92_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6580187.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1147..1521)
                     /locus_tag="POB92_002766"
     CDS             complement(1147..1521)
                     /locus_tag="POB92_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDI6580188.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          569 a          211 c          255 g          587 t
ORIGIN      
        1 ttggaataac ttttggtaat ttataaataa tttaaaaaaa ttgtttataa aatggaagcg
       61 tatatagaat gaaggttggg tatatagttt attgagggag gtgtcacaat gaataaagtc
      121 acaattaatc ctcaaatcca attaacttat caaattgaag gtaaagggga tcctataata
      181 ttacttcatg gattggatgg taatttagct ggatttgaag atttgcaaca tcaactagca
      241 tcatcatata aagtacttac ttacgattta agaggtcatg gcaagtcttc taaaagtgaa
      301 tcatacgatt taaacgatca cgttgaggat ttaaaaattc taatggagaa gttaaatatt
      361 catgaggcac atattctagg acatgattta ggtggggtag ttgctaagtt atttacagat
      421 aaatatgctt atcgtgtaaa atcattaact accattgcat cgaagaaaga tgacttaata
      481 cacagcttta ctcaattgtt aatacaatat caagatgata tagcgggttt taataagtct
      541 gaagcgtata ttcttttatt ttctaaattg tttagaaatc aagagaagac gatgaaatgg
      601 tatcaaaaac aaagaatata tagcattaag tctgaggatg atagtgcggt ggcaattcgt
      661 tcattaattt tgcataaaga tgaacctatg tatttaaaaa aacgtacatg tgtacctact
      721 ttgttaatta atggggaaca tgatcctttg attaaagata agaatcattt taaattggaa
      781 gcgcattttt taaatgttac gaaaaaaatc ttcgaacatt caggacatgc accgcatatt
      841 gaagaaccag aagcatttat gaattattat ttaaattttt taaaaagcgt atcataatat
      901 gtgatatata aacctagggc ataaagtctt taggcaatgt gaaaaagctg attactattc
      961 attatttgat agaaatcagc tttttttgaa atgtatttga tatatactgc tcgttatgcg
     1021 gctatcttcc ttatattaag tgccattagt gcaaaacctc ttaacattta caaaaaaaga
     1081 cataaaaaaa ggaagtttaa tagaatgtat catctatcaa acttcaccaa attgcgctaa
     1141 acaaaattat agttcaattt cgttgtttgc ttcagtgatt cgtttattta ctcgactcaa
     1201 taatgattcg atttttttac gttgttgtgc attaacaaga attaatacag ttctttcatc
     1261 atgctcatta cgttttttat cgaagtaatc ttcttgagat aaaattttaa ctgctttaac
     1321 aacttgtggt tgtttgtagt ttaaatgatt aataatatct ttaagatagt attctttctc
     1381 tttgttttcg ctgatgtatg tcaatacagc gaattcttca aagctaattg aaaattcctt
     1441 tttaattaaa ctttttaatt tgtcagcata agtgaccatt gataacaact caaagcaatc
     1501 attgattttt gtaattgcca tgtttaaaac ctccctattt gatgcatctt gctcgataca
     1561 tttgcccgat aatatattga tatctaatct ttatttatta tagatatgtt agtcataatt
     1621 tt
//
LOCUS       DAMZLE010000257         1580 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMZLE010000257 DAMZLE010000000
VERSION     DAMZLE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.263"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            173..1516
                     /gene="brnQ"
                     /locus_tag="POB92_002767"
     CDS             173..1516
                     /gene="brnQ"
                     /locus_tag="POB92_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6580189.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          504 a          239 c          252 g          585 t
ORIGIN      
        1 ctatttaaaa agtaaatttg aagaatttta ttttctggca taatttgatt taaacaacac
       61 atgtgttttg tttctgctat gtacaacaca ttccatatag actctttatt attttttgtg
      121 ctaaaatatt tacttgtaaa ttatttcgtg ttttaaaaaa aggagtaact ctatgaataa
      181 aaatacatgg gtcattgggt ttacgctctt cgctatgttt tttggcgcag gcaatctaat
      241 ttttcctcct aatctcggat tggatagtgg tcaatttttc tggcccgcca ttttagcgtt
      301 tgttctaact gggattggtt taccattatt aggtgtgatt gtaggtgcac ttgataaaga
      361 aggatatatt ggcgcattaa ataaaatttc acctaaattt tcaatattgt tcttaatcat
      421 catttatttg actataggac cacttttcgc aatacctaga actgcatcta catcttttga
      481 aatgacaatt acaccaatta tacatagcaa tagtagtatc gctttattta tatttacgat
      541 tatctacttc atagtcgttt tgtatatttg tttaaatcca tctaagttaa tcgatcgtat
      601 tggttcatta ttaacaccat tattattgat tactatttta gcgatgatta ttaaaggata
      661 cttagacttt agcggtaata gtgctggaaa gggcaatgaa gcactatatc attctaattt
      721 ttcaagtttt gctgaaggct ttacacaagg ctatttaaca atggatgcca ttgcagcaat
      781 tgctttttca atgattgttg ttaatgcagt aaaactaaca ggcattacta aaacaaatca
      841 aatattcaaa caaactttga ctgctggttt aattgcagcc gtagctttaa ttttcatata
      901 tatttcatta ggttatattg gtaatcatat gccagtaagt gacatgacgt tagatcaatt
      961 gaaatccaaa gatcgaaaca ttgggacata tttattaacg acaatggctt caacaggatt
     1021 tggttcattc ggaaaatatt tattgggcat cattgtggct ctggcatgtc taactacagc
     1081 atgcgggctt attgttgcag tttctgaata tttccataga atcgtaccta aagtatcata
     1141 caaagcattt gtattagttt tcattttaat gagttttatt attgctaacc aaggtttaaa
     1201 tgctgttatc tcaatgtcaa ttccggtatt aagcattgta tacccagtag caataactgt
     1261 tgtattatta attttaattg ccaaattcat accgacaaaa cgcatttcac aacaaattcc
     1321 agttattatc gtatttatat tgtcgatttt cagtgttatt agtaagttag gttggctgaa
     1381 aattaacttt atagaatcat tgcctctaag agcgtattct ttagagtggt tcccagtagc
     1441 aattattgca acgatattag gctatctagt cggcatattt gtaaaacaag atccaattaa
     1501 atatcaacag gaataacgaa taatataaaa gaggttggga cataaatccc taaaaaaaca
     1561 gcagtaagat aattttcaat
//
LOCUS       DAMZLE010000258         1557 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMZLE010000258 DAMZLE010000000
VERSION     DAMZLE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.264"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..>1557
                     /locus_tag="POB92_002768"
     CDS             71..>1557
                     /locus_tag="POB92_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDI6580190.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEP
                     SVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQSNPEDLKVSEVTRFNNKSIGKNELTGALQLKNKVS
                     FKNDFEFNIRVANNHQSVTTGADGWGFLFSKGDGNEYLQKGGILGPKGMENSAGFKID
                     TGYNFKDPMDKEEKQAGQGFKGYGTFVKTGADGTTAKVGTNIPTRGKADNSFQYADNS
                     IDTTDGKFHGQLLNNLKLAYNEKSGIMRAEYAGKIWEANISDLGLDKSEAYNFLITSS
                     QRQGTSQGVYANGWMRTDLNNSTFKLTPNLVNKTETKTVEIPFETKREFNPKLQPGEE
                     RVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIV"
BASE COUNT          647 a          218 c          315 g          377 t
ORIGIN      
        1 attgtatttg taaaattaca tgtaaattca ctgtaagtaa agtggaaaat atggaaaaag
       61 gagtatgcaa atgagagata agaaaggacc ggtaaataaa agagtagatt ttctatcaaa
      121 taaattgaat aaatattcaa taagaaaatt tacagttgga acagcatcta ttttaattgg
      181 ctcactaatg tatttgggaa ctcaacaaga ggcagaagca gctgaaaaca atattgagaa
      241 tccaactaca ttaaaagata atgtccaatc aaaagaagtg aagattgaag aagtaacaaa
      301 caaagacact gcaccacagg gtgtagaagc taaatctgaa gtaacttcaa acaaagacac
      361 aatcgaacat gaaccatcag taaaagctga agatatatca aaaaaggagg atacaccaaa
      421 agaagtagct gatgttgctg aagttcagcc gaaatcgtca gtcactcata acgcagagac
      481 acctaaggtt agaaaagctc gttctgttga tgaaggctct tttgatatta caagagattc
      541 taaaaatgta gttgaatcta ccccaattac aattcaaggt aaagaacatt ttgaaggtta
      601 cggaagtgtt gatatacaaa gcaatccaga ggatctaaag gtatctgaag tcactcgttt
      661 caataataaa agtattggaa aaaacgaatt gacaggagca ttacaattaa agaataaagt
      721 ttcttttaaa aatgactttg agtttaacat tagagtagct aataatcatc aatcggtaac
      781 aactggcgct gatggatggg ggttcttatt tagtaaaggt gacggtaacg aatatttaca
      841 aaagggaggt atattaggtc ccaaaggaat ggaaaattca gcaggattta aaattgatac
      901 agggtataat tttaaagatc ccatggataa agaagagaaa caggcaggac aaggatttaa
      961 agggtatggt acgtttgtaa aaacaggtgc tgatggaact acagcaaagg ttggaacaaa
     1021 cataccaaca agaggtaaag cggataattc attccaatat gcagataatt ctattgatac
     1081 aacagatgga aaattccatg gtcaattact taataattta aaattagcgt acaatgaaaa
     1141 atcggggata atgagagccg aatatgctgg taaaatttgg gaagctaata tatcagattt
     1201 gggattggat aaatctgaag catataactt tttaattact tctagtcaaa gacagggaac
     1261 atctcaaggt gtatatgcaa atggttggat gagaacagat ttaaataact caacatttaa
     1321 actaacgcct aatctagtta ataaaacaga aacaaaaaca gtagagatac cgttcgagac
     1381 aaaacgtgaa tttaatccga agttacaacc tggtgaagag cgagtgaaac aagaaggaca
     1441 accaggaagt aagacaatca caacaccaat cacagtgaac ccattaacgg gtgaaaaagt
     1501 tggcgaaggt caaccaacag aagagatcac aaaacaacca gtagataaga ttgtaga
//
LOCUS       DAMZLE010000259         1515 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMZLE010000259 DAMZLE010000000
VERSION     DAMZLE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.265"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..369)
                     /locus_tag="POB92_002769"
     CDS             complement(<1..369)
                     /locus_tag="POB92_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6580191.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDS"
     gene            complement(362..529)
                     /locus_tag="POB92_002770"
     CDS             complement(362..529)
                     /locus_tag="POB92_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580192.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(504..1355)
                     /locus_tag="POB92_002771"
     CDS             complement(504..1355)
                     /locus_tag="POB92_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDI6580193.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          546 a          247 c          174 g          548 t
ORIGIN      
        1 actatctact tgcgaaccaa taataggtat caataaatta acaattaacg taataacacc
       61 tactacagct agataaataa tagaaatacc tgcaactctt ggtattctat aacgttccat
      121 catatttacg attgggttaa atagatagaa tagaattaaa gagacgatga taggtgctgc
      181 tatcgtctta aaaacgatga taaaaggatc aaaaacatat gatacttttt cgaaaataaa
      241 aataacaata cccaacaata ctagcgctat taatgagaag actaaatcat tcccaccaac
      301 aaacttcata taccgtgatt cggtaaattt taaaaaattc tttcttatat tcttttcatt
      361 ttcattcaat tatagattca ccaccactta aaatattcat aagatgttgt ataaaaacag
      421 tccacttatg taaaattact aacatcaatg ataccataca aatacccttt acagtaacga
      481 aacgcccgtg taatacactt aatttaggat tgattaatcc tttcttcaaa atgatgaatg
      541 tgtaatgtta aatatataat ttcagactca ctgatattaa catcaaattg tttttgtatc
      601 atagttaaaa ttttatatgc tgtgttatag caaatcggat agtgattttt aatcatagac
      661 acaaaatcat cttgtgcatg tatatattct tttcttctta atcggcgaat taaaaattgt
      721 acgtgcctta taaaacgttg gtattgtaat gattccttat caacagttgt cacaaggtct
      781 gattcaatga tatctatacc ttttttaata acattattga tcaaggtcat ctcatgcata
      841 gataaatctt ctgtattaga tgcaatatgt aatgcaataa atcctatctc atcttcagga
      901 aaatgtacat ctaatgctgc atttaactga tcaatcacct gtttagcaat atgatatgca
      961 tcactatata actgcatagt ttccataaca aatggattgc taataacttg attttgtttt
     1021 aagcgtttat aagcaaatat aatatgatcc gttaatgaaa ctacaagttg ttttgaatca
     1081 acattcatcg cagtattaga aataaaattc aacgaatcaa taattacttg taatacatta
     1141 tcatcagcga tttcaactaa acttttataa tgtgcttttt gttgctcact ctctaattta
     1201 taaattttct ctattgtaat agtttggtcg tttaacgcca ttccctcttt tttgttaaaa
     1261 ccaatacctt taccgattaa aataacttct tgatcattat tagtacatac tacgacattg
     1321 ttgttcaatg ttttagtaac aatatattct cccattatca tcacctattt ttctattatt
     1381 aagattatat atcggaaatg tctaacttga aagtataata atttaaatac ttgtgttcta
     1441 ttacacgcct atcctatatg atatatctta atttaatttc gatgcctctc aaagtggaat
     1501 aactataata aaaaa
//
LOCUS       DAMZLE010000260         1479 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMZLE010000260 DAMZLE010000000
VERSION     DAMZLE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.266"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            52..468
                     /locus_tag="POB92_002772"
     CDS             52..468
                     /locus_tag="POB92_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6580194.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            538..1386
                     /locus_tag="POB92_002773"
     CDS             538..1386
                     /locus_tag="POB92_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6580195.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
BASE COUNT          574 a          212 c          267 g          426 t
ORIGIN      
        1 taaagcaaca ttacttaaga tgccaccaac ataactgaaa ggatagtcgc aatgaaaact
       61 aaagaagtcg tagcgctcat gaatatatct caagacactt taagatatta tgaaaaggtt
      121 ggtgtgattc cacctgttaa tcgagatgaa aatggatata gaatatataa tgatagtgat
      181 ttaaattgga tttatttagt gaaaaatttg cgaaatgcag gcgtcagtat tgaatcgctt
      241 atcgaatttt gcaggttagc gcagcttcct aaaaatgaaa atattcaagc acagcaaaag
      301 caaattttaa ataagcaact cgaagaatta aatgaaaact taaagacaat tcatgatgcg
      361 agagatttac tacaatataa aattgataat tatgataatc atattgctaa aatcaatgct
      421 agtgataatc atgatgacaa tgttgaacgt ctttgggaga gaaagtaaac ctatggcttg
      481 ctatagagtt cgctccatgg tttatagtgg acttacaaga taaaggaggt tcattttatg
      541 aatcatattg aaataagtaa agatgtaaag ataccagtat taggcttcgg agtatttcaa
      601 attcctcaag aacaaacagc agaagcggtt aaagaagcaa ttaaagcggg ctatagacat
      661 attgatacag cgcaaagtta tttaaatgaa actgaagtag gtcaaggtat tgaagcttca
      721 ggtattgata gaagtgaact attcattacg actaaagtat ggattgaaaa tgtaaactat
      781 gaagatacta tcaaatcaat cgaacgatca ttacaacgat taaatttaga ctatcttgat
      841 ctagtgctta ttcaccaacc ttataatgat gtatatggct catggcgtgc attagaagag
      901 ttaaaagaaa atggtaaaat taaagcaatc ggtgtatcta acttcggcgt tgatcgaatt
      961 gtagatttag gtattcacaa tcaaattcaa ccacaagtga accaaataga aattaatcca
     1021 ttccatcaac aagaagaaca agttgcagca cttcaacaag aaaatgttgt ggtagaagct
     1081 tgggcaccat ttgcagaagg taaaaatcaa ttattccaaa atcaattgtt gcaggccatt
     1141 gcagataaat ataacaaatc aattgcacaa gttattttac gctggttagt agaacgtgac
     1201 atcgttgtac tcgcaaaatc agtgaatcca gaacgtatgg ctcaaaactt agatatattt
     1261 gattttgaac ttacagaaga agataaacaa caaattgcga cattagaaga aagtaatagc
     1321 caatttttct cacatgctga tccagagatg attaaagcac ttacaagcag agaattagat
     1381 gtttaatcat aatataacaa agtaaatcat ttacattacg ctaacctgaa cttggatgtt
     1441 aaagtctatg tttcaggtta ttttattact cttaaaagt
//
LOCUS       DAMZLE010000261         1466 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMZLE010000261 DAMZLE010000000
VERSION     DAMZLE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.267"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            277..>1466
                     /gene="eap"
                     /locus_tag="POB92_002774"
                     /gene_synonym="map"
     CDS             277..>1466
                     /gene="eap"
                     /locus_tag="POB92_002774"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HDI6580196.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGG
                     KRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLK
                     ISNK"
BASE COUNT          644 a          191 c          206 g          425 t
ORIGIN      
        1 taaagatgct gtaaatataa ttgacaaatg taatcggaat taagaaatga agattgttat
       61 gacatttaag tttgaaactt ggtataaaca aaatcaaaca acattttagg tattgtaaat
      121 tagttagtta atcaaaatta aaagaacttt taacagtgtt aaaaaattat tgttaaagat
      181 aatttattat taatattcag ttaattcaaa aaatagagaa agtctggcta taataaagtt
      241 gcaatcacga attacataaa aaaggagaga taatttatga aatttaagtc attgattaca
      301 acaacattag cattaggcgt tatagcatca acaggagcaa actttaatac taacgaagca
      361 tctgccgcag ctaagccatt agataaatca tcaagtacat tacaccatgg acattctaac
      421 atccagattc catatacaat tactgtgaac ggcacaagcc aaaacatttt atcaagctta
      481 acatttaata agaatcaaaa tattagttat aaagatatag agaataaagt taaatcagtt
      541 ttatacttta atagaggtat tagtgatatc gatttaagac tttcaaagca agcggaatat
      601 acggttcatt ttaaaaatgg aacaaaaaga gttatcgatt tgaaatcagg tatctacaca
      661 gctgacttaa tcaatacaag tgacattaaa gctatcagtg ttaacgtaga tactaaaaag
      721 caacctaaag ataaagctaa agcaaatgtt caagtgccat atacaatcac agtgaacggc
      781 acaagccaaa acattttatc aaacctaaca tttaataaaa atcaaaatat tagttacaaa
      841 gatttagagg gtaaagttaa atcagtttta gaatcaaata gaggtattac tgatgttgat
      901 ttaagacttt cgaagcaagc gaaatataca gttaatttta aaaatggaac gaagaaagtt
      961 atagatttga aatcaggtat ttacacagcg aatttaatca attcaagtga tattaaaagt
     1021 atcaatatta acgtagatac aaaaaaacat atcgaaaata aagctaaaag aaactatcaa
     1081 gttccatatt caattaatct aaatggtaca tctacaaaca ttttatcgaa tctttcattt
     1141 tcaaataaac cttggacaaa ttacaaaaat ttaactagtc aaataaaatc agtactgaag
     1201 catgatagag gtattagtga acaagattta aaatatgcta agaaagctta ttatactgtt
     1261 tattttaaaa atggtggtaa aagaatctta cagttaaatt caaaaaatta cacagcaaac
     1321 ttagttcatg cgaaagatgt taagagaatt gaaattactg ttaaaacagg aactaaagcg
     1381 aaagcagaca gatatgtacc atacacaatt gcagtaaatg gcacatcaac accaatttta
     1441 tcaaaactaa aaatttcgaa taaaca
//
LOCUS       DAMZLE010000262         1457 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMZLE010000262 DAMZLE010000000
VERSION     DAMZLE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.268"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            371..769
                     /locus_tag="POB92_002775"
     CDS             371..769
                     /locus_tag="POB92_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDI6580197.1"
                     /translation="MKKNITKVIIASTVIATGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERADKLIKEADEKNEHYKGKTVEDLYVIAKKMGKGNTIAVVKIKDGGKNGY
                     YTFDITRPLEEHRKNIPVVKNGEIDSITWY"
     gene            720..821
                     /locus_tag="POB92_002776"
     CDS             720..821
                     /locus_tag="POB92_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580198.1"
                     /translation="MFQWLKTAKSIRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1139..1456)
                     /locus_tag="POB92_002777"
     CDS             complement(1139..1456)
                     /locus_tag="POB92_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HDI6580199.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKVEKYNEYQTNFK
                     KQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYN
                     TLK"
BASE COUNT          524 a          222 c          201 g          510 t
ORIGIN      
        1 gtgtcatttc tttgttttat aaacattaat tatcaaaaaa gctatatgta taacaatttg
       61 gagtgtttgt ctacccaaca ttgcaaacaa aatgcaaaga aatatatatc gttttaacaa
      121 taaaaattat ccaatcatag ttgagtgacg tttactacta ttattatact atcaacaaat
      181 tcaataatac ctcaaataaa aataatacca tcaaattcat cattattctt tttcgattgc
      241 attaaacctt attaatttgc acttaatttt aattaatgaa gtgttaactg gtaaatgcat
      301 gaaaccgttt gtaaaatagc agtgtactcg cattagtaag tacaaattta ttttacaggg
      361 agaacttatt atgaaaaaaa atatcacaaa agtaattatt gcatctacag tcatcgctac
      421 aggtcttcta actcaaacta atgatgctaa ggcgttcttt agttatgaat ggaaaggttt
      481 agaaatcgct aaaaatttag cagaccaagc taaaaaggac gatgaacgtg ctgataaatt
      541 aataaaagaa gccgatgaaa aaaacgaaca ctataaaggt aaaactgttg aagacttata
      601 tgtaatagct aaaaagatgg gtaaaggcaa tacaatagct gtagtaaaaa tcaaagacgg
      661 tggtaaaaat ggttattata ccttcgatat cactcgccca ttagaagaac atagaaaaaa
      721 tattccagtg gttaaaaacg gcgaaatcga ttcgattact tggtattaaa aaacatactg
      781 aattaaatag ttgtacgcca aacgttagaa aacaatgcta acgttttggc gtgctttttt
      841 tattttaagt aacttctaat ttatttagca tctttacgac tgtttaataa agcacgtatg
      901 attaacacgg ttgccactat atccgttaca atttttatga ttgttaatac attcgatttc
      961 cttttcacaa tagccacctc gcttgttcaa aacataaaca actattgcat tcacttttaa
     1021 gtaattattt atattattta tcccaataag ctcaccattc aaataaacac aatattataa
     1081 aataattata cctttggata tagcaaaaag ccacactcta tagcatggct tctatcaatt
     1141 attttaaagt attatatttt aaaactagat cgatttgtct ttgtaatttt ttcttttcat
     1201 agctgtgttg gaaatgaatt aagttaacag ctctttgtgc tttacggtgt gttgcaacag
     1261 ttcttgtacg tttgaacaag tttacagctt tttgtgcgtc cacaactttt ttatttactt
     1321 gttttttaaa gtttgtttga tactcgttat atttttcaac cttagtttgt gcatgagatt
     1381 caccggcaaa cgttgatacc gttaaactaa tagcagctat gcttaaaatt gatttcccaa
     1441 taaaattctt tttcata
//
LOCUS       DAMZLE010000263         1456 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMZLE010000263 DAMZLE010000000
VERSION     DAMZLE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.269"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            11..1156
                     /locus_tag="POB92_002778"
     CDS             11..1156
                     /locus_tag="POB92_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6580200.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            1140..>1456
                     /locus_tag="POB92_002779"
     CDS             1140..>1456
                     /locus_tag="POB92_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDI6580201.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNY"
BASE COUNT          608 a          160 c          226 g          462 t
ORIGIN      
        1 gtgaagtaat atgattcgaa ttcaagataa tacaataagg gatggtatgc aacaaagtaa
       61 tgttcgaaaa agtctaatta taaaaaaaga agtattgaaa caaattaaca agttaaatat
      121 aaattctgtt gaagtaggca tgtgtacaac tatcgaggat gaatttaata ttcatcaatt
      181 cagagacatt ttaagtcctg aaaaagaatt agtagtattg actaggctta atgaaaaaga
      241 aataaaaaaa atagtcaaat tgaaaattca taatttagtg gtaaaaatac tattgccaat
      301 atctgacttg catataaaag aaaagcttaa tttttcaaat aaatattata ttcagaaaat
      361 caaagactgc ttggatatat taaagaaaga taaaaaagga gtagatattt gttttgaaga
      421 tgcaacaagg acttctagag aaaaattgaa agaatacatg gaaattattt caaaatatca
      481 agttagaaca gttacatttg cggacactgt aggatgttcg acaccattag aatacggaga
      541 tatttttaat tactttgtaa aaaaatattc taacataatt ttttctgctc attgtcataa
      601 cgatctaggg ttggctactg caaatacatt agctgcaatt ttaaatggtg caaagcaaat
      661 agaaactaca tttttgggaa ttggtgagag agcgggtaat gctcctattg aggaaataat
      721 tactattttg acaaaaaaac aaatagaaag tacggaattc actttacccg acgtatataa
      781 aactagtatt aatatttcta aaattctcga ttttcaaata tcagaaaaca aacctataat
      841 tggtgaaaat atatttaaac atgaatcagg aattcatcaa gatggtacta aaaaaaatat
      901 aaatatgtat caatatttag ttcctagtga tttaggattg aaaaattcac aagttgttca
      961 gtttccaata agtaatattt ctagtaagaa aatcttgcac aataaattta aatcaatagt
     1021 taacactgaa gaaattgatg aaaatatttc tttctataaa cttgtgaaca agttttcacc
     1081 tgaagtagca cctgaagata cagtggattt acttcagata ataaaaagga ggagtaaaga
     1141 tggaaatttt aaatgattct gtaaaaagtt ttgaaaaact ttatttacaa ttacaagatg
     1201 gttttccagt gattgttcct actgatacta attataattt atgtagttta cctaacaacg
     1261 atctttgtat cgataaaata tttgaatata aaaagcgatc gaaagataag ccattatcat
     1321 tatttattga taagccagag gattggaaat tgtatggaga taatcaaaat acggaaatag
     1381 ttgataaact agttgaaata ttttggcctg gaccattaaa tattatttta aaaaataaaa
     1441 caagctataa ttatat
//
LOCUS       DAMZLE010000264         1420 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMZLE010000264 DAMZLE010000000
VERSION     DAMZLE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.270"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            47..1393
                     /locus_tag="POB92_002780"
     CDS             47..1393
                     /locus_tag="POB92_002780"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI6580202.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
BASE COUNT          519 a          192 c          283 g          426 t
ORIGIN      
        1 ttatgttata atatttgtat tgttagtata tatgggggcg tttcaaatgc attttattgc
       61 aattagtata aatcatcgca cagctgatgt tgcactaaga gagcaagttg cttttagaga
      121 tgatgcctta cgaattgccc atgaagattt atatgaaact aaatctattt tagaaaatgt
      181 catattatca acatgtaatc gaactgaagt atatgctgtt gttgatcaaa ttcacacagg
      241 tcgttactat attcaacgat ttctagctcg tgcatttgga tttgaagtag atgatattaa
      301 agcaatgtca gaagtaaaag tgggggacga agcagtagaa catttattgc gtgtcacttc
      361 tggtttagat tcaatcgtac ttggagaaac tcaaatttta ggtcaaataa gagatgcatt
      421 tttcttagcg caaagcacag gtacgacagg aacaattttt aatcatctat ttaaacaggc
      481 aattactttt gcaaaaagag cacataatga aacagatata gctgataatg ctgtaagtgt
      541 gtcttatgct gcggtcgagt tggcgaaaaa agtatttggc aaattgaaaa gtaagcaagc
      601 tatcattatt ggtgcagggg aaatgagtga attatcacta ttaaatcttc ttggttctgg
      661 aattactgat attacagtag taaatagaac aattgaaaat gctatgaaat tagcagcaaa
      721 gcatcaagtg aaatatgatg aactatcatc attaccaaat ttacttgaaa gtgcagatat
      781 tgtgattagt tcaacgagtg cacaatctta tatcattaca aatgaaatga tagaaagaat
      841 tgcagaaaat agaaagcaag attcactagt attgattgat attgcagttc ctcgagatat
      901 tgaacctggt attagtgcca tcacaaacat ctttaattat gatgttgatg acttaaaagg
      961 tttagttgat gcaaacttac gtgagcgaca attagcggct gcaacaattt cggaacaaat
     1021 tcctgcagaa atacatgcac acaatgagtg gattagtatg ttgggtgttg ttccagtgat
     1081 tagagcttta cgtgaaaaag ctatggcaat tcaagcagaa acgatggaca gtattgatcg
     1141 taaattgcca gggttaagcg aaagagagcg taaaattata tctaaacata caaaaagtat
     1201 catcaatcaa atgttgaaag atcctattaa acaggccaaa gaattaagta gtgataagaa
     1261 aagtaatgaa aaattagagc tatttcaaaa tatatttgat atcgaagctg aatgtcctca
     1321 tgagcaagcg aagcaacaaa aagaaagcaa ggtcaaggaa atttcagcgc gacgtatctt
     1381 tagttttgaa taagcatatt aatatggtga taatatgcaa
//
LOCUS       DAMZLE010000265         1397 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMZLE010000265 DAMZLE010000000
VERSION     DAMZLE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.271"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1142
                     /gene="scpA"
                     /locus_tag="POB92_002781"
     CDS             <1..1142
                     /gene="scpA"
                     /locus_tag="POB92_002781"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDI6580203.1"
                     /translation="ALSLILSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSIPTEVR
                     NLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYI
                     VTISPKVTKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLV
                     LKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYVNKLENFK
                     IRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMI
                     YFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAK
                     LDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
     gene            1173..>1397
                     /locus_tag="POB92_002782"
     CDS             1173..>1397
                     /locus_tag="POB92_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDI6580204.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVI"
BASE COUNT          542 a          205 c          217 g          433 t
ORIGIN      
        1 tagctttaag tttaatactt tctttaagtg ttacccctat tgcaaacgct gagagcaatt
       61 caaatatcaa agcaaaagat aagaagcatg tgcaagttaa tgtcgaggac aagagtattc
      121 ctactgaagt acgtaattta gcgcaaaaag actatctttc ttatgttact tcacttgata
      181 aaatctataa taaagagaaa gcctcgtata cacttggtga accttttaaa atttataaat
      241 tcaacaaaaa gagtgatggt aattattatt ttccagtttt gaacactgaa ggtaatatcg
      301 attatatagt tacaatttca ccaaaagtca ctaagtattc tagttcatct tctaaataca
      361 caatcaatgt ttctccattt ttatctaaag tcttaaatca atataaagat caacaaatta
      421 ctattttgac aaactcaaaa ggttactatg tggttactca aaatcataaa gctaaactag
      481 tactaaaaac accacgtcta gaagataaaa agttaaaaaa gactgagagt atacctactg
      541 gcaataatgt aacgcaacta aagcaaaaag cttctgtcac tatgccaact agtcaattta
      601 aaagtaataa ttatacttac aacgagcaat atgtaaataa acttgagaat ttcaagataa
      661 gagaaactca aggtaacaat ggttggtgtg caggctatac gatgtctgca ttactaaatg
      721 cgacttataa tactaacaaa tatcatgcag aagctgtgat gagattttta cacccaaact
      781 tacaaggaca acgattccaa tttactggtt taacgccaag agagatgatt tattttggac
      841 aaactcaggg aagaagtcca caacttctta atagaatgac tacatataat gaagttgaca
      901 atcttactaa aaataataaa ggaattgctg ttttaggtag tagagtagaa tcaagaaatg
      961 gtatgcacgc agggcatgcc atggcagttg ttgggaacgc taaattagat aatggacaag
     1021 aggttataat tatatggaat ccatgggata atggttttat gactcaagat gctaaaaaca
     1081 atgttattcc agtatccaat ggcgaccatt atcaatggta ttcatctatt tatggttatt
     1141 aaaatttgta ttttagaaag gtaaataata ttatggaaca atttgaatta tttagtattg
     1201 ataaattcaa atgtaattca gaggctaagt attatcttaa tattattgag ggagaatggc
     1261 atcctcaaga tttaaatgat agtcctttaa aatttattct cagtacctca gacgattctg
     1321 attacatttg caaatatata aatacagaac acaaacaact cacattatat aataaaaata
     1381 atagctcaat tgttatt
//
LOCUS       DAMZLE010000266         1355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMZLE010000266 DAMZLE010000000
VERSION     DAMZLE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.272"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1318
                     /locus_tag="POB92_002783"
     rRNA            <1..1318
                     /locus_tag="POB92_002783"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DAMZLE010000267         1353 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMZLE010000267 DAMZLE010000000
VERSION     DAMZLE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.273"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(57..275)
                     /locus_tag="POB92_002784"
     CDS             complement(57..275)
                     /locus_tag="POB92_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580205.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(337..621)
                     /locus_tag="POB92_002785"
     CDS             complement(337..621)
                     /locus_tag="POB92_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580206.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            complement(709..1278)
                     /locus_tag="POB92_002786"
     CDS             complement(709..1278)
                     /locus_tag="POB92_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580207.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
BASE COUNT          446 a          200 c          196 g          511 t
ORIGIN      
        1 atcttgtttt catttaaaag gcgaaacatt aaaataatta aataaaaata ttgcgtttaa
       61 tttacagcgt caaatatact tatttctaat gctttggggt ctactgaaac aagtaaagaa
      121 tgatcgatgt tactaatatt gccattctcc aaatttattt ctgtgagtat ttggaagcta
      181 ccattaggca acggtttaac aatagacaat tgcttttccg cttgttgtat taaaaaaggt
      241 tttgtagatt gattattaat atgccattca ctcatgtatg tttttcactc ctgctttaaa
      301 atagggttag aaagtttata gttgagacat tcatgttcaa ccaaaatttt gttcgaattc
      361 aataaatgtc ttgtttaaaa tagaaatatt gtaaatgtta tcgtccaaaa cttcaccagt
      421 taagtatttg ttttgaatta aaatttggca gttagttaag aagtcttgat aatcacgatc
      481 acaaaaatag ttttcacgtg catctttagc atcgccaaaa aagttagcga ctgtttctgt
      541 ttctccatta ttcgaacgtt caatatataa tttgtaaaat ttagctattg tatacttttg
      601 ttctttagtt agttcattca aaatattggg cctcctgaaa tatcatttgt aatctatacc
      661 caatttattg caaaacacaa actaatttaa ctatttgatg aaactgtgtt aataagcttt
      721 aacaagcctt agtttgtatg gatctataaa attatcttta attgcatagg gtgaaataat
      781 atgtaatcca taacttttaa ctgatttttc acttacacca aatttataag cttggtagat
      841 aattttagta caatacgtaa attttttgct gttcaaattt aatgtaacta gataacgatg
      901 atttgtattc tcatagtttt tcttaaccca ttcagccgct tttttacctg caccaggata
      961 gctgcaacga taaactttca tccaatcatt tttgccactt gcataattat atttaaaaga
     1021 ttcgaaggat tgtgtagttg gtttgtcgcc aggcccctca atttgtaaaa tcgttttatc
     1081 atcaatcgcg atactacaat gaccaaaaaa tccccacatg acagggcctt ttgtaacaat
     1141 aatatcacca ggttgtaatt ggaatttgtc atcttgaatt tctgaatact tattatctgc
     1201 aattgttttt ggtgagttta ttggggatac gacaacgaat aatataagta aaattatcgt
     1261 tcgtttaata tagttcactt aaaagctcct tgttgaagaa atatatgtaa atagtcttaa
     1321 attagaattg taatctttaa taagcttgta aga
//
LOCUS       DAMZLE010000268         1292 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMZLE010000268 DAMZLE010000000
VERSION     DAMZLE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.274"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            55..686
                     /locus_tag="POB92_002787"
                     /pseudo
     CDS             55..686
                     /locus_tag="POB92_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
     gene            921..>1292
                     /locus_tag="POB92_002788"
     CDS             921..>1292
                     /locus_tag="POB92_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580208.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSS"
BASE COUNT          475 a          146 c          210 g          461 t
ORIGIN      
        1 ggaattgaaa agttttaata ggaataataa acaaaggaat ggtagtgata aatattgcta
       61 tgtgaatcta aagttatcaa caaaaatcct aagtatagag ttattaaata tggtgatgaa
      121 tatttaatga ttgatttagt aagtacctgg ttaactttat ttcttcctat gattaattgg
      181 ttaattccaa aaaaatatgt caaaatcagt aaaaaagaat ttgacgattt aaacattgtc
      241 aaacctgtta aaaataaagc tttttggcca gttgcaggta gtactatttt gttcggagtt
      301 acgtttagaa aatatattcc ttcacttaat attcaattag agaaaaacat ggtgattgta
      361 atatgttgtg caatatttct gggtgtttta atactttttt tatttctgaa tcgtaagcta
      421 aggttggaaa tttataataa taactctagt aaagggaaaa taattttatt tccttcatta
      481 aaaaactttt gtttcacaat attttattat tttttatttg gcggtctttc aataatggct
      541 ctaagtatgt tattaacttt aaatcctcaa aatataatag gctttattgg ttggtaatga
      601 ctgcaggttt ctttctgtta aacatgtcat cgattattga caaaaaaatt tatgtattat
      661 ctaaaactaa cacggtggaa aaatgatggt ttagctggat ttactgcagg ttctatttcg
      721 gcaatacttg tatattagac caatcaaaaa aatgaatttg gaataaaaga taaaaacgat
      781 tggataggac ataaactaga cgttggtata gatgctgtag aaaaatctgc agaaaaaaca
      841 gtagatggtg ttgaaaatgt catggtgaag cttcaaaaag tatttctaat catataagcc
      901 ctaagaaatg gagctggtaa atgttgctat gcgaatctaa aatcatcaat aaaaacccaa
      961 aatatagaat tattaaatat aatgatgaat acttaatggt cgatataata agcacttgga
     1021 ttagtttatt ttttcctttt attaattggt tcatcccaaa agaatacgtc aaaattagta
     1081 gagaagagtt tgaaaactta aatattgtta aacctgctaa aaagaatgtt ttttggccag
     1141 ttgcaggtag ctctgctttg ctgggagttg cattaagaaa atatacacat ttacttgaca
     1201 ttcaacttga taaaaaatta gttattgcca tatgttgcat cacatttata gggattttaa
     1261 tattttatgt acgcctaatt aaaaaatcat ct
//
LOCUS       DAMZLE010000269         1285 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMZLE010000269 DAMZLE010000000
VERSION     DAMZLE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.275"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            27..206
                     /locus_tag="POB92_002789"
     CDS             27..206
                     /locus_tag="POB92_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580209.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNSESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            517..777
                     /locus_tag="POB92_002790"
     CDS             517..777
                     /locus_tag="POB92_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580210.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(830..1180)
                     /gene="scn"
                     /locus_tag="POB92_002791"
     CDS             complement(830..1180)
                     /gene="scn"
                     /locus_tag="POB92_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HDI6580211.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          403 a          165 c          164 g          553 t
ORIGIN      
        1 tttaaacaaa aggaagtaat aaaaacgtga aatttaaaac aactaaagaa tgtaaaagta
       61 ataatatctt taaaagaagt caagaaatta ataatagtga aagtgaaaag ggttgtttat
      121 ggggcattag catgttgatt ctacttttct tattgattct gtttggaata actgcttgtt
      181 catcaagcat tcactttatt aattagattt ttttacttgg aggtatcatg tgaagaacca
      241 tacaaatata attaatatct tattagttat agtcaactca ttaactcatt ttctaactct
      301 aaacacctca ttttttaata attcagcatc ggatttctgt tttatcatag gggctatatt
      361 tttcttgatc ggaatttttg ttgcaatata cggtatgaag cgagcaacat attggttaaa
      421 cttattgatt ttatttacca atatttttta ttttctacac ttctgtgttt tacttttgtt
      481 aaaatatata ggatttaaat tatttattta tgaagggtgt gtattgttat ttacctaatt
      541 tatagtctaa ttgtctattt catctgcatt ataaattttt tcataatggc cgcaccattc
      601 ttaatgacta atgctgatta tgtatggacg cctatgacta cagttacgtt atttattttg
      661 agtttgatta ttttcttgat atttataaaa acaaaagatg tcgttcattt aacaattttc
      721 atattaaact tacttttttc aatactttat tgtttaccta tactgtttta tttatgaaca
      781 cttttatatt atctagaaaa ttaaaaccac ccttttacgg gtggtttttt taatatttac
      841 tttttagtgc ttcgtcaatt tcattataaa tcttttgaag ttgatatttt gcttctgaca
      901 ttttcttaaa gtcttttgac ttaagagcat acattgcttt ttgacctgaa atttttatag
      961 ttcgcttata ataagtgttt aaacttccag tagctaattc attaacattt agttcatcta
     1021 ataacgattt taattcatta gctaactttt cgttttgata ttcattcgat gttggcaagc
     1081 ttgtgctagc ttgtgcctca tttttatcta gattagttac tagtggtgat gctaaaacga
     1141 ttgctaaagt tcccgcaagt atagattttc taattttcat tttttattct ccttagtaat
     1201 tagtactaat ttattttaat gactaactaa aaaaaagtac attaattatt cattaactat
     1261 atagatcttc ttattaaatg aaaat
//
LOCUS       DAMZLE010000270         1274 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMZLE010000270 DAMZLE010000000
VERSION     DAMZLE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.276"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            180..488
                     /gene="rplU"
                     /locus_tag="POB92_002792"
     CDS             180..488
                     /gene="rplU"
                     /locus_tag="POB92_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI6580212.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            494..814
                     /locus_tag="POB92_002793"
     CDS             494..814
                     /locus_tag="POB92_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDI6580213.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            826..1110
                     /gene="rpmA"
                     /locus_tag="POB92_002794"
     CDS             826..1110
                     /gene="rpmA"
                     /locus_tag="POB92_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI6580214.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT          453 a          183 c          263 g          375 t
ORIGIN      
        1 ttgcagaagt tattgaaaga aagcgattgt caatcaaggc gttgaaggaa gaaacgagaa
       61 gagtttatgt ttataaatga ctcgagttgc gaccgaaaca acgtagagtg caaacgcttg
      121 actcaataac gaaatgtcac ctatgtatgg cgtgacttat tatacaggag gtgcaaagta
      181 tgtttgctat tattgaaaca ggtggaaaac aaatcaaagt agaagaaggt caagaaatct
      241 tcgttgaaaa attagacgta aacgaaggag atacttttac atttgataaa gtattatttg
      301 taggtggaga ttcagttaaa gttggagcgc caacagttga aggtgcaaca gttactgcta
      361 ctgttaataa acaaggtcgc ggtaaaaaaa tcactgtatt cacatacaaa cgtcgtaaaa
      421 attcaaaacg taaaaaaggc catcgtcaac catacactaa attaacaatc gataaaatca
      481 acgcgtaatt attatgatta ctgttgatat tacagttaat gatgaaggca aagtaacaga
      541 cgttattatg gatggccatg ctgaccatgg tgaatatggt catgatatcg tttgtgctgg
      601 agcttcagct gtattgtttg gtagtgttaa tgcgattata ggattgacat ctgagagacc
      661 agatatcaat tatgacgaca atggtggtca ttttcatata agaagcgttg atacaaataa
      721 cgatgaagcg caactaattc ttcaaacaat gcttgtgtct ttacaaacta ttgaagaaga
      781 atataatgag aatattagat taaattataa gtgaggtgca ttccgatgtt aaaattaaac
      841 ttacaattct tcgcatctaa aaaaggggta agttctacaa aaaacggacg tgactctgaa
      901 tcaaaacgct taggtgctaa acgtgctgac ggtcaattcg taacaggtgg ttcaatttta
      961 tatcgccaac gtggtactaa aatttaccct ggtgaaaatg taggtcgtgg tggcgatgat
     1021 acattattcg ctaaaatcga cggcgttgtt aaattcgaac gtaaaggtcg cgacaaaaaa
     1081 caagtttctg tatatgcagt agctgaataa ttttgtctag ttaacaccag aagtgaatct
     1141 tctggtgttt tttacttttt ataaaaggtt tttcattatt attttaatta tgttattaaa
     1201 aaatgggaat tttagacgtt atactaaatg tacaactgca taagagcccc taatcgctaa
     1261 actataaggg gaca
//
LOCUS       DAMZLE010000271         1233 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMZLE010000271 DAMZLE010000000
VERSION     DAMZLE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.277"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            527..628
                     /locus_tag="POB92_002795"
     CDS             527..628
                     /locus_tag="POB92_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580215.1"
                     /translation="MKFVANKLTLNIFRKFCINNEKEIWIKYEEVFL"
     gene            638..910
                     /locus_tag="POB92_002796"
     CDS             638..910
                     /locus_tag="POB92_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6580216.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
     gene            954..>1233
                     /locus_tag="POB92_002797"
     CDS             954..>1233
                     /locus_tag="POB92_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6580217.1"
                     /translation="MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAT"
BASE COUNT          473 a          161 c          193 g          406 t
ORIGIN      
        1 aactttgaca tagaatgatg gttaaagaaa atctcattta gtcaccgtct ttttaacggg
       61 ctcattaggc gacatgttcc tgcatgtcgt ctttttacat ttttatagta acatatcttt
      121 tatttaattg tctattttta ttagatgtgt aacaaaattt tgaagtattt tccgtatatt
      181 gcctgctaaa catgtcagat ttgaaaggaa aatcatactg tggtagaaat caattatgat
      241 tatatggtag tacttataaa taagtgattt ctattgaata tgtaatgaag ttcatatatt
      301 aatataactt tcattgatat agaagtcgca atgattaatt taaacaataa aaaacaacca
      361 tcatttcaaa actttgacaa cgaatgatgg ttaaagaaaa tctcatttag tcaccgtctt
      421 tttaacgggt tcaatagggg acatgttcct gcatgtcgtc tatttatgca ctgcgcaatt
      481 taacaataat acaattatac tttgatgtcc atttaacata aagtggttga aatttgtagc
      541 aaataaatta accttgaata ttttcagaaa attctgtata aataacgaaa aagagatatg
      601 gattaaatat gaagaggtgt ttttatagaa gaataaaatt tttggcacta tattatattt
      661 aactttagca cttggattat caacagcagc ttatgcatct acagaatacg cagaaggagg
      721 cacttggagt cacggtgtcg gcagtaagta tgtttggtct tattattatc atggtcataa
      781 aggacatggt gcaacagcta ttggaaaata tagatcattt agtggttata caagagctgg
      841 tgtaaaagca aaagcatcag ctactaaaca taattgctgg gtcaatagag cgtattataa
      901 catttattaa atactaaaaa atcatagaaa ataaacaacg caagaggtac aacatgaaat
      961 ggttcaaatt aatacttgat gttacaacct tcattctgat tgctatattg ttatttgttt
     1021 atacatataa agaaaacgaa gaaatattgc ctgatactaa ataccctata gcggtaactg
     1081 actggaataa aaaatatagt aaaaatgaga tttataaacg tataaatcaa ttcgctaaaa
     1141 atgagaacgt agcaatctat aaatcaactt caaattatac aaacaaaaac gtagataaag
     1201 atatatatgt atttaataaa tcaaaagcaa caa
//
LOCUS       DAMZLE010000272         1217 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMZLE010000272 DAMZLE010000000
VERSION     DAMZLE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.278"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(104..1114)
                     /locus_tag="POB92_002798"
     CDS             complement(104..1114)
                     /locus_tag="POB92_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDI6580218.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
BASE COUNT          473 a          154 c          170 g          420 t
ORIGIN      
        1 cttaagcttt atttgaaaaa cgaaaaatgt ctaaatacac tgtagtaact acttttcaaa
       61 atgaataaag tggttacagt taatgtactt agacagtata aaattatgaa tctttaaact
      121 gtgatggtct ttgtaaatta attattggat ttgtccattt acaaacaaag tttgtagata
      181 atacatatac gatgattaca gatatactta ttaaataaag ataagtcatt aatgatatag
      241 gattatcgaa tgggtaccat tcaaatcctc taacaatgcc aataattaaa ccatgtaata
      301 aatatacgta tagcgtacga ctaccaatat aagtatataa ttttttcttt gttgacatta
      361 aatttagaaa cgcagtcatt gcgattaata taattccata taatataagt cgtttaaaag
      421 gactgaatat actctgtcct tcattttcaa gtgaagtata tggtgaactt cccaataacc
      481 aatctgcatt gataggatga atcacgtaaa cgataaaaaa caaaataaag gtaatgatag
      541 atactggtat tagtttttta tttttaaaaa tagccgtatg ttttttggtg aaaatgtaac
      601 ctagataaaa tattgggaaa aatacgattg tccttgaaat gcttaagtag ctatcgatgt
      661 tatctgaaaa acctgctcca atagatataa taattgaaac tgatagcact ttatatggat
      721 taaatcttct aactattact aaaatgacat gaaagaaaaa tagcgtgatc aaaaaccata
      781 acgcaaatac tgggttaaaa ggatcaagtt gtaattcgtc acttttacct gttaagaaat
      841 aataaattga aaagaatgca aaaaatatca tataaggtac tatcaaacgt tttgaaattt
      901 tttctaaata gtatggttta tcaatatttt tcgcgaaata accagatata aacaaaaatg
      961 ttggcatatg gaaactataa ataactaggt ataatgctga taaatattta tcgccactag
     1021 tgtagggttg taacatatgt ccaaatacga ctaataatat taaaattgct cttgcgttgt
     1081 caaaaaaata atctctctct tttaatgaag tcatatttta tctcctttat gtccataaga
     1141 ctcttactaa tttattatat tttgtattag ttatgcaaca aatttgtcta ttgtgtcgaa
     1201 tagtattgta aaaatta
//
LOCUS       DAMZLE010000273         1212 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMZLE010000273 DAMZLE010000000
VERSION     DAMZLE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.279"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>1212
                     /locus_tag="POB92_002799"
     CDS             <1..>1212
                     /locus_tag="POB92_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580219.1"
                     /translation="QVDDILKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQ
                     DDTSEDDDNESEKADSKSADSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTD
                     MMTKKGKGSNDTLNREEGDAVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQ
                     DVQYEIKDLIQIIKKTIEREQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKS
                     FDATFTLLIDASASMHDKMAETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHAQP
                     NIINEIINYDYSTFEKDGPRIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSD
                     GEPSAFNYSQDGIIDTYEAVEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIF
                     VEGVAHLPGHL"
BASE COUNT          459 a          168 c          238 g          347 t
ORIGIN      
        1 atcaagtaga tgatatttta aaagaagata tgctaaacga atattattac ttacctaaaa
       61 cactatataa tacattagct agtccagagt ttgatgattt aaagagaact gatgcaagtc
      121 aagtcgatgg gcaagatgat acttcagaag atgatgataa cgaaagtgaa aaagctgact
      181 ctaaatctgc agatagtgag tcaaaaggtg gcgcctattt agaaatggaa cttcacgaag
      241 gacaaaatag tgaaacattg ggtaatgatg aagcacgtga aggcgatgct acagatgata
      301 tgactgatat gatgaccaaa aagggcaaag gttcaaacga tacgctaaat cgtgaagaag
      361 gcgatgctgt tggtcaaagc caagcatttc aacttgatgg tgtgaacaaa aatgtagaaa
      421 taaaatggca aattcctgaa attgagccac aatatgtttt agaatatcaa gaatcgaaac
      481 aagacgtaca atatgaaatc aaagacttaa ttcaaattat aaagaaaacg attgaacgag
      541 aacaacgaga tgcacgtttt aatttaacaa aaggacgatt acaaaaggat ttaattaatt
      601 ggtttatcga cgatcaatat aaattgtttt acaaaaaaca agatttaagt aaatcatttg
      661 atgcgacgtt tacattgtta attgatgcgt cagcaagtat gcatgataaa atggctgaaa
      721 cgaagaaagg tgtagtgtta ttccacgaga cgttaaaagc attgaacatt aaacatgaaa
      781 ttttatcatt cagtgaggat gcctttgatt cagacgatca tgcacaacca aatatcatta
      841 atgaaattat taactatgat tactcaacct ttgaaaaaga tggcccacgt attatggcac
      901 ttgaacctca agatgataat cgtgatggtg ttgctattag agttgcaagt gaacgattaa
      961 tgcgtcgaaa tcaacatcaa cgatttttaa tcgtattttc agacggcgaa ccgtctgcat
     1021 ttaattatag tcaagatggt attattgata cgtatgaagc tgtagaaatg tcacgtaaat
     1081 ttggtattga agtatttaat gtatttttga gtcaagatcc aataactgaa gatgttgaac
     1141 aaacaattca taatatttat ggtcaatatg ctattttcgt tgaaggtgtt gcgcatttac
     1201 ctggacatct ct
//
LOCUS       DAMZLE010000274         1206 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMZLE010000274 DAMZLE010000000
VERSION     DAMZLE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.280"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            174..971
                     /locus_tag="POB92_002800"
     CDS             174..971
                     /locus_tag="POB92_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI6580220.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNSSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          431 a          211 c          185 g          379 t
ORIGIN      
        1 atgaaccgct atcctaaaat gttaataata ttacaagata ataacaaacc acacaaagct
       61 acttattttt gataatatgg aaatcgtaat ataaaacgaa aacttaattt actatataaa
      121 ttgtcttaat aatttttaaa agtagtaaaa cttaatttta aggaggagtc cctttgaaaa
      181 aattagcatt tgcaataaca gcaacatctg gtgcagctgc atttttaacg catcatgatg
      241 cacaagcttc tacacaacat acagtacaat ctggtgaatc attatggagt attgctcaaa
      301 aatacaacac ttcagtagag agtattaaac aaaataacca attagataac aacttggtat
      361 tccctggtca agttatctca gtaggtggaa gtgatgcaca aaattcgtca aacacttctc
      421 cacaagctgg ttcagcatca tctcatactg tacaagctgg tgaatcatta aatatcattg
      481 ctagcagata tggtgtttca gttgatcaat taatggcagc caataactta cgtggttatt
      541 taattatgcc taaccaaaca ttacaaattc ctaatggtgg atcaggtggt acaacaccaa
      601 cagctacaac aggtagcaat ggcaatgcat catcttttaa tcaccaaaat ttatacactg
      661 ctggtcaatg tacatggtac gtatttgacc gtcgtgctca agctggtagt ccaattagca
      721 catattggtc agacgctaag tattgggctg gtaacgcagc taatgatggt taccaagtaa
      781 acaacacacc atcagttggt tcaattatgc aaagcacacc tggcccatat ggtcatgttg
      841 cttatgttga acgtgtcaat ggtgatggta gtatcttgat ttctgaaatg aattacacat
      901 atggtccata caatatgaac taccgtacaa ttccagcttc agaagtttct agctatgcat
      961 tcatccatta attaaataaa ttgtactgat atatactagc aattcacatc atgtgagatt
     1021 gctagttttt tatttttgaa aaaaattttc attttggtac aaaaaattat ctcacccttc
     1081 cctatcatac atatttatat tttgtatgaa tggtagttag gtaaaaatta acaacctacc
     1141 tatttgatta aattaaatgc ataatatatt aaaaatcaaa ataatgcaac taagttgact
     1201 atataa
//
LOCUS       DAMZLE010000275         1185 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMZLE010000275 DAMZLE010000000
VERSION     DAMZLE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.281"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..87
                     /locus_tag="POB92_002801"
     CDS             <1..87
                     /locus_tag="POB92_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDI6580221.1"
                     /translation="MTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            175..792
                     /locus_tag="POB92_002802"
     CDS             175..792
                     /locus_tag="POB92_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580222.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVISTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            867..>1185
                     /locus_tag="POB92_002803"
     CDS             867..>1185
                     /locus_tag="POB92_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580223.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCM"
BASE COUNT          445 a          135 c          190 g          415 t
ORIGIN      
        1 atgacaatgc tatttttctt tataaatatg tcttcaataa tagacaaaaa gattcatgtt
       61 atatatttaa ggtcatataa atattaattt tgatacaaaa gggcacaagt gttttgagaa
      121 acagaaacga taatatagct gtgtgaattt gttattaata ggaagtgata attattgctt
      181 tgtgaaaccg aaattatcaa taaaaatccg aaatacagag ttattaagta tgatgatgag
      241 tatttaatgg tcgatgtaat aagcacttgg cttgtatact tttttccatt tattaattgg
      301 ttcattccaa aaaggtgcgc caaaattagt agagaagagt atgaaaaact gaacactgtt
      361 aagccagtta aaaataaaaa tttttggcct gttgtaggag gaacaatcct actaggtgct
      421 acttccagaa agtacataca cttacttaat attcaattag aaaaaagatc ggttatattt
      481 atatgcttcg ttgtatttct atgtatttta atcttttttg tgcttctgaa tcgaaaatta
      541 aaattgaaag ttttcgataa taaaaaagaa gaacaaaaaa taattttagt accaacatta
      601 aaaaatgctg ttcttatatt atatggctat ttgttgattg gaggcatgtc aatattagct
      661 ttaagcatgt tgctgacatt agaaaaccag aatttaataa cttttatagc ttggggtatg
      721 gggttaatgt tgtttttctt gatgaatata acactcattg tcaataaaac agtcaaagtt
      781 ataaaaaggt gaataaataa ctaattatat tgtattggcc gttcttcaaa taaaaaataa
      841 tttgaataag gtggaagtga taaacagtgc tttgcgaatc tagagtcatt aatcaaaacc
      901 ctaaatatag aattattaaa tacaataatg aatatttcat ggttgattta gtaagtactt
      961 ggattgctta ttttttgccc atgattaatt ggtttattcc caaaaagtac gcgaaaatta
     1021 gtagagaaga atttgaaagt ttaaatattg tcaaacccgc taaaaataat actttctggc
     1081 ctgttgcagg atttgcagtg ttattaacaa ccttaacaag aaaatatatc tatttgctta
     1141 acatccattt agaaaaagaa atagttatat taacatgctg tatga
//
LOCUS       DAMZLE010000276         1159 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMZLE010000276 DAMZLE010000000
VERSION     DAMZLE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.282"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>1159
                     /locus_tag="POB92_002804"
     rRNA            <1..>1159
                     /locus_tag="POB92_002804"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          350 a          223 c          327 g          259 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaaggacg acattagacg aatcatctgg aaagatgaat caaagaaggt
      361 aataatcctg tagtcgaaaa tgttgtctct cttgagtgga tcctgagtac gacggagcac
      421 gtgaaattcc gtcggaatct gggaggacca tctcctaagg ctaaatactc tctagtgacc
      481 gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg agtgaaatag
      541 aacctgaaac cgtgtgctta caagtagtca gagcccgtta atgggtgatg gcgtgccttt
      601 tgtagaatga accggcgagt tacgatttga tgcaaggtta agcagtaaat gtggagccgt
      661 agcgaaagcg agtctgaata gggcgtttag tatttggtcg tagacccgaa accaggtgat
      721 ctacccttgg tcaggttgaa gttcaggtaa cactgaatgg aggaccgaac cgacttacgt
      781 tgaaaagtga gcggatgaac tgagggtagc ggagaaattc caatcgaacc tggagatagc
      841 tggttctctc cgaaatagct ttagggctag cctcaagtga tgattattgg aggtagagca
      901 ctgtttggac gaggggcccc tctcgggtta ccgaattcag acaaactccg aatgccaatt
      961 aatttaactt gggagtcaga acatgggtga taaggtccgt gttcgaaagg gaaacagccc
     1021 agaccaccag ctaaggtccc aaaatatatg ttaagtggaa aaggatgtgg cgttgcccag
     1081 acaactagga tgttggctta gaagcagcca tcatttaaag agtgcgtaat agctcactag
     1141 tcgagtgaca ctgcgccga
//
LOCUS       DAMZLE010000277         1158 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMZLE010000277 DAMZLE010000000
VERSION     DAMZLE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.283"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..1002)
                     /locus_tag="POB92_002805"
     CDS             complement(<1..1002)
                     /locus_tag="POB92_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDI6580224.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     "
BASE COUNT          447 a          214 c          143 g          354 t
ORIGIN      
        1 acacaattca tgaacaataa atgccgatac aaatggtatg acaaaataac caagtgctaa
       61 taaaataata ttcattgttg gatcacctga cattcggtta aatgcattta ttgggccaac
      121 taatcctgta tatccaaatc cagcagacaa tggtgttcct ttaacctgaa gaacgtaagc
      181 aatgattcca gtaattattc catttatagt caatggtatc gaaataatta aatttttcaa
      241 gtaaactggg atcatcattt ttgcagctcc tatgagtaaa actgcattta caccaataga
      301 attgacacgc aatgagccaa ataagaaagt aacacaggca gccactatac ctaggtttgc
      361 tgctccactt cctagtccgt ttaaactaat cgcagttgca atcgctacta acgatattgg
      421 tgttaccatt aataatgaaa atgccacact aataagtata gacattaaca acggatttaa
      481 gtctgtaaaa gaatgaatta catttccaat tgcttgagta atttttcgaa tgtaaggtaa
      541 tgtgattaga ccgatacccc cactaacgat aggtactaaa actggtaata taattaattc
      601 aaaagatcca agtttgtttt gtaataccat atatataaga catgcaataa taacaaccaa
      661 actcgtattt ataatgtcac ctataccttt taacataaaa ctattattgc tatatacaac
      721 agcacctgaa ccaatcatag ctgatgtacc tactatagca gcacctgcac cattaaattt
      781 aaattgatga gcagctaaaa ccccaataat aaatgccata aatgattgaa ttagtatcac
      841 taactgatac gttaattcta aaatttcatt accactttta aatattttta atacttcacc
      901 taataaagca ttcggaacaa gtgcaataac aacaccagca ccaatagaat ttaaaatctt
      961 actgaaaaac tgtttattat cagcattatt tgcgttactc ataaacgacc tccaatttga
     1021 acattaaact catcatatta tagaaaatta actttaacaa tgattagttg gctaatttaa
     1081 tctaaatata acaatccaca aattgaataa agcaaattta aacaatctac tcactaatca
     1141 atgtcatgtt aattttaa
//
LOCUS       DAMZLE010000278         1149 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMZLE010000278 DAMZLE010000000
VERSION     DAMZLE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.284"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(363..>1149)
                     /locus_tag="POB92_002806"
     CDS             complement(363..>1149)
                     /locus_tag="POB92_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6580225.1"
                     /translation="IANNTFFSSYYSQINDWLNSLPKKIPSMLSEFTDGFGSKIATFA
                     ETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTNIMPPKFRKDFHDLLEKMSVQVGSYI
                     QGQIIVSFCIGILLFIGYSVIGLKYSLVLASIAAVTSVVPYLGPTIAISPAIVIAAIT
                     SPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLKIHPLTIIFILLCAGKLLGIVGVILG
                     IPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEYDIKESNKIVE"
BASE COUNT          434 a          190 c          156 g          369 t
ORIGIN      
        1 ttaagaagta aatagaaaat atttctatct caaacagaac aatatggtta tctatactga
       61 ttcatattta acataaagaa aatgatgaat caatacttat gaaaaatatt attaattaac
      121 agccgattat gagtgcttct taatatatta aaaataccac ttgtttttat tttcgacata
      181 ataatgaagt actaatcaag tggcaaatac tcgtttcatt atgcatgtgc tttataaacg
      241 gctaaacttt cgtaaaaatt tataagctaa acatgtgctg ataatcataa atgtaactgc
      301 ccaacattat aagcaccata aaaaatacca acaaacatat agtcattggt attttttatt
      361 acttattcaa ctattttatt actttcttta atatcatatt cacctacatc attaccatag
      421 aaacgattgt atcgacgttt aaataattgg aataaatgag taactaatac ttttaaaata
      481 gcatatcccg gaatacctaa aataacgcct acaataccaa gcaatttgcc tgcacacagt
      541 aaaatgaaaa tgattgtaag tggatgaatc ttaagtgttt tacccatgat atttggtgaa
      601 atgaagtgcc cttcaacaaa ttgtactaaa gtccatacta ctgctaattt taagagcatc
      661 cacggcgatg ttatagcagc tattacaata gctggagaaa tcgctatagt aggccctaaa
      721 tatggtacaa cacttgtaac tgccgcaata ctagctaata ctaagctata tttcaacccg
      781 ataaccgaat aaccgataaa caacagtata ccgatacaga atgaaacgat aatttgtcct
      841 tgaatgtatg aaccaacttg aacactcatt ttttcaagta gatcatgaaa atcttttcgg
      901 aatttcggtg gcataatatt cgttgaaaat tctttgaagt gatgtccatc tttaagcata
      961 aagaatagta caaatggtgt tgtgacaatc acaacgccaa tattagcaat cgtttctgca
     1021 aacgttgcaa ttttagaccc aaagccatct gtaaattcac ttaacataga tggtattttc
     1081 ttaggtaaag aatttaacca atcattaatt tgactataat acgaagagaa aaacgtatta
     1141 tttgctatt
//
LOCUS       DAMZLE010000279         1125 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMZLE010000279 DAMZLE010000000
VERSION     DAMZLE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.285"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            420..904
                     /gene="tnpA"
                     /locus_tag="POB92_002807"
                     /pseudo
     CDS             420..904
                     /gene="tnpA"
                     /locus_tag="POB92_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          421 a          156 c          231 g          317 t
ORIGIN      
        1 aaaaagatat aagaaaaaga cggataatta atccgtcttt ttttatgaaa agaataggct
       61 gggaaataaa tcaatcttct atgatctacg aagttatatt ggcagtaatt gactgaacga
      121 aaatgcgctt gtaataagct tttttcaatt ctagtcaggg gccccaacac agagaatttc
      181 gaaaagaaat tctacaggca atgcgagttg gggcggggcc ccaacaaaga gaaattggat
      241 tcccaatttc tacagacaat gcaagttggg gtgggacggc gaaataaatt ttgcgaaaat
      301 atcatttctg tcccactccc cagaaaaggt tctaccattg tcaaaaaaat gcatctctac
      361 atgttagagt aaatattggt cagccaacca aaataatcaa cacgaggaga tgctatttaa
      421 tgtcatctga cacaaacagt ttagcacata cgaaatggaa ttgtaagtaa catattgtct
      481 ttgcacctaa atacagaaga caagcgatat atggaaaaat aaaaaaagat atagggatta
      541 tattacgtca attatatgaa agaaaaggtg tagagataat tgaagcagag gtatgtaaag
      601 atcatatcca tatgttagta agtataccac ccaaacttgg ggtatcatca tttgttggct
      661 atttaaaagg aaaagtaatt taatgatatt tgatagacat gctaacttaa agtatagata
      721 tggaaataga aagttttggt gtaaaggatt ttatgtggat acagtaggta gaaataaaaa
      781 agtgattgaa aattatattc gtaatcaatt acaagaggat atcgttgcag atgaaatctc
      841 aatggaagaa tatttagatc ctttcactgg agagaaaaat aaaaaaagaa agaaaaaaga
      901 gtaaaccttt aggattgctg gaatagtagt gcagttggct gacttgtcag tgccctttta
      961 gggctggcca gtaaggaagg cttatagccg cagaacaaac cacccgttca cacgggtggt
     1021 ttttatttgt aacatctgtt gataggtatc gacaaggacc ttttaatgtt gtgttttgtg
     1081 cgatataaca agctttttag gctattaaag aatattcaga attaa
//
LOCUS       DAMZLE010000280         1122 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMZLE010000280 DAMZLE010000000
VERSION     DAMZLE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.286"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(184..1092)
                     /locus_tag="POB92_002808"
     CDS             complement(184..1092)
                     /locus_tag="POB92_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6580226.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDDKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
BASE COUNT          320 a          215 c          126 g          461 t
ORIGIN      
        1 aggtatacta ctttagctat aataatacta atctatcatt aattaaattg aacgataaca
       61 acggcactta ttattaaaaa aaatacaaat atctaacttt ctagctataa aaataagagc
      121 ctaatacaat atcaaaagat tgatatgttt cagactctca tttcactaac ttatatagct
      181 gaattatttt gcagctttaa ttaatttttc ttttaaatct ttacgcatga aatctaatgt
      241 ataaggatcg ttgtaccagt atgtaccagc atcaacttta acaatatgtc cttctttagt
      301 agctttcaaa ttcttccaca tgtttgttga ttcatatcct ggtgtaggtt taccttcact
      361 tgtactcaca atgtaatcac cagcatattt ttcaatttct tcttgtttca cttcagccca
      421 acctgctttt gcagttaact tttgttgttc tggttgcatt tttaaaccaa atgcttgata
      481 taatacttct ccaccacgac cccagttatc gccgtaagtg tataattttt tatcaaattc
      541 atcaaacaat gacactgttg catcttgtcc aattgctttt ttaatttctt tatcgtcttt
      601 agcagttgtt tcttcccaat ctttcttcca agcttttact ttatcttctt taccaacaat
      661 tttccctaac atttcttgtt gttctaaata tttatgctta ttatagtcaa caactactgt
      721 tggtgctact ttttgatatt ttttaatatc tttgtcagta gagtatacaa taattaaatc
      781 tggcttttct ttagcaactt tttctacatc gccatcacca atttttgtaa cacctttaaa
      841 tttatctttt aatactttgc tttgatcgac ttgttgattt acagctacaa tgtttgcacc
      901 taatttttta agtccaccag catatgttgg cgcaactact gcaatgcgtt tagggtcttt
      961 cggaatatct accgttttgc catcgtccat tttataagat ttagtttcag ctttgttatt
     1021 tttttcacct tggttcccac acgcagctaa cactaataac ataattatta atggtaataa
     1081 tagttttttc ataatttcct cctattgaaa atgattatca at
//
LOCUS       DAMZLE010000281         1008 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMZLE010000281 DAMZLE010000000
VERSION     DAMZLE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.287"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            67..501
                     /locus_tag="POB92_002809"
     CDS             67..501
                     /locus_tag="POB92_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6580227.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(758..943)
                     /locus_tag="POB92_002810"
     CDS             complement(758..943)
                     /locus_tag="POB92_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDI6580228.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT          380 a          143 c          163 g          322 t
ORIGIN      
        1 gaaacttttc aatttgtgtt atattgattt tgtaagatac tttaactcac aaaggagaga
       61 gattatatga aattaaaatc atttataact gtaactttgg cactgggcat gatcgcaacg
      121 actggcgcta ctgtggcagg taatgaggta tctgcagcag aaaaggacaa actaccggca
      181 actcaaaaag ctaaagaaat gcaaaatgtt ccatatacaa ttgcagtaga tggcattatg
      241 gcttttaatc aatcttactt aaatttacca aaagatagcc aattatcata tttagattta
      301 ggaaataaag ttaaagcttt gttatatgat gaacgcggtg taacacctga gaagattcga
      361 aatgcaaaat ctgccgttta cacgattact tggaaagatg gtagtaaaaa agaagtggat
      421 cttaagaaag atagctacac agcaaacttg tttgattcaa attcaattaa acaaattgat
      481 attaatgtaa aaactaaata aacacttaaa attatcaaat tcaacatgaa gcatttaatt
      541 ttacagtgat gattataaaa taattgcctt gatacaaaga ttactcgtaa atgacatctt
      601 tgtattaagg ctttttctaa atttaaaagt gatgggttag aggtcattga gctttaaaat
      661 attcaaaata caaaacatta atggccaaaa ataaaagccg cctttatctg ggcagcttca
      721 ataataagaa agacatattt cattttatac taaatagtta ttgtgatgaa tctttcggcg
      781 gtttaattac tgcagcaaaa attgctgtga aaatcgtgaa caatactgcc atgataattg
      841 gattcactac atttaagctg tctccaccta ctaggctatt aagtacaaag ttaaccattt
      901 gcattaataa taatgcccaa aagaatgtta cgaggtgttt catgtcattc tacctccact
      961 ttaattatat atattttatt ttaagtgaaa gttagaaatt tgtatagt
//
LOCUS       DAMZLE010000282          974 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMZLE010000282 DAMZLE010000000
VERSION     DAMZLE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.288"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            18..872
                     /locus_tag="POB92_002811"
     CDS             18..872
                     /locus_tag="POB92_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580229.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
BASE COUNT          370 a          132 c          154 g          318 t
ORIGIN      
        1 aaaggattgg tgaaaaaatg tctttttatg ttgtacttat cattatcatt gtagcattaa
       61 ttggtatatt agttttaaat caaaggtata gtaatagtaa aatagatacc gaagtttatg
      121 caagaaagca acttattaaa aagaacaaag cattaagtgc agaaaatgct gaattaagaa
      181 gtcaaatgct gagttctaat aatgatgttg gccaccatgc atacaaaaat gctaaaagag
      241 aattacgcaa gattttagat agctatctcg aaaatggtaa attaaaatat tacgatatta
      301 tcgttacaag caatttagct actaaacatc cgtttttcga gtatgcacgt tcatttgatt
      361 ttatcattgt ttcagatatc ggtttaataa acgttgatgt aaaaagctgg ggtgaaaaaa
      421 cattttatca ctttgatgta cctgatgagc atgatacaga aataagcaat agtaatattg
      481 aaaaagttgt cggtcattac attagccagc aatatcatga tcagtttaat tcatcaagaa
      541 agtctatcta tacttttaca gaaacagttc aaccaaatcg tgttatttat gatttttatg
      601 attatgatcc gtatcaatta gcagcaaata atgcgaaagc attaaaggat catattgaac
      661 aaaactttaa ctttaaagtc caaagtaccg gtgttattta ctttagtgat ggtacagtta
      721 atatcattca aggttctgaa gaaagagata aatatgttga tacagtttca acaaaatctt
      781 cgttacgacg tattattagc gaagccatag agctatctaa acacccttta aataaagaac
      841 aggttgatca aatcacagct atattcaagt aaaatcaaag gcataataat ccattacgct
      901 cgatacgcac tatggttatt atgcctttaa attatttcta tatataaatt tatggtaata
      961 ataaatcgat gctt
//
LOCUS       DAMZLE010000283          958 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMZLE010000283 DAMZLE010000000
VERSION     DAMZLE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.289"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(263..751)
                     /locus_tag="POB92_002812"
     CDS             complement(263..751)
                     /locus_tag="POB92_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580230.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTREGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGVLPETEYEMEEIREVEKYVKD
                     QE"
     gene            complement(762..>958)
                     /locus_tag="POB92_002813"
     CDS             complement(762..>958)
                     /locus_tag="POB92_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580231.1"
                     /translation="DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEY
                     EINKVKEIEQYIKEQDEAEL"
BASE COUNT          304 a          187 c           91 g          376 t
ORIGIN      
        1 catatttttt tgatataagt tttaaaaaat aagtcgatgt gcatcaactt tataaaatac
       61 actacaataa ccttttcaat taatggcaat tttaaattta tatcgtgatt tattaattag
      121 ttaaactcat ttatacatat gatgtatgga taccgagaag ctaatagact ttttaagttg
      181 caaataatct tatcgtttac cttcttcttt gagtcaaatg taaaactagg gtgattttgc
      241 acagccttct ataacatata tgctactctt ggtctttaac atacttctct acttctcgaa
      301 tttcttccat ttcatattcc gtttctggta aaacaccaaa ctttttatac atatagtagt
      361 tttcctttcc tgatggacca aaacctaatt taatccaatc tatataatca aacgatactt
      421 tcaaattacc ctctcttgta aaatcaaatt cacatgatgt ccatggttct aaatcttctt
      481 ctttaaatgc gtttcttaac ttcttaaaca atctatataa atccgtccac aaatcataaa
      541 atactttttc agagacatta tactctctag ggatatatgt gtaataattc aattcatcac
      601 tacctggttt agtataatta aaaacgactt ctccaccttc atcatctaca taagcaattg
      661 tatatacttt ttcccactct actggtatca tcccactaat ctcattcgca atctcgttgt
      721 acatttgact taatttttct tcgaaagtca tgttatcgcc cctatagttc agcttcatct
      781 tgctctttaa tatattgctc gatttcttta actttattaa tttcgtattc catttctggt
      841 aaaacaccaa actttttata catataatag ttttcacggc ctaattgatc aaactctgta
      901 tttatccaat caatataatc aaatgaaact tttaatttac cttcgcttgt aaaatcaa
//
LOCUS       DAMZLE010000284          945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMZLE010000284 DAMZLE010000000
VERSION     DAMZLE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.290"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            22..867
                     /locus_tag="POB92_002814"
     CDS             22..867
                     /locus_tag="POB92_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6580232.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFLRIIEQLN
                     RRYGK"
BASE COUNT          378 a          117 c          189 g          261 t
ORIGIN      
        1 tgttatcaaa taaaaagtga tgtgagtgaa ttgtcaaaaa gtgaagatca acgtattact
       61 aaaacaaaag atgaacaaat taagcaaata gatatatcgg atatcaaacc gaatccgtat
      121 cagccccgaa aaactttcga tgaaaatcat ttaaatgatt tggcagattc aattaagcaa
      181 tatggaattt tgcaaccaat tgtgcttaga aaaacagttc aaggttatta cattgtagtt
      241 ggtgaaagaa ggtttagagc ttcgaaaatt gctggtctaa aatacgtatc agcgattatc
      301 aaagatttaa cagatgaaga tatgatggaa ctggcggtca tcgaaaattt acaacgagaa
      361 gacttaaatg cgattgaaga agctgaaagt tatcaacgtt tgatgacaga tttgaaaatt
      421 acacaacaag aagtagcgaa gcgattgagt aagtcgcgcc cgtatatagc gaatatgttg
      481 aggttattac atttgccgaa aaagattgct gacatggtaa aagatgggcg actgacaagt
      541 gcacatggac gaacgttatt ggcaattaaa gatgaacaac aaatgcttag gttagcgaaa
      601 cgggttgtta aagaaaagtg gagtgttaga tatttagaaa accatgttaa tgaattaaaa
      661 aatgtttcgt caaagtcgga aacagacaaa gtagatataa ctaagcctaa atttataaag
      721 caacaagaac gacagttgcg agaacagtat ggtaccaaag tagatatatc aataaaaaaa
      781 tcggttggta aaatctcatt tgagtttgat tcacaagaag attttctaag aataattgaa
      841 caattaaatc gtaggtatgg taaatagtta cacaatttta tataataact ctttgtgcaa
      901 gtgtaaataa attgtaatca gtgaacattt gattctagat atatt
//
LOCUS       DAMZLE010000285          919 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMZLE010000285 DAMZLE010000000
VERSION     DAMZLE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.291"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            83..>919
                     /gene="fmtA"
                     /locus_tag="POB92_002815"
     CDS             83..>919
                     /gene="fmtA"
                     /locus_tag="POB92_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDI6580233.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NIL"
BASE COUNT          381 a          113 c          141 g          284 t
ORIGIN      
        1 taagttgtat tattaggcat ctaggttcaa atttttaacg atttaaatat ataaaatgaa
       61 tacattgtga ggcaattatc aaatgaaatt taataaagta aaactagtta tacatgcgtg
      121 tgtactatta tttatcatta tttctatagc gttaattttt catcgattac agacgaagac
      181 acattctata gacccaatac ataaggaaac aaaattatca gacaatgaaa aatatttagt
      241 ggatcgtaat aaggaaaagg ttgcgccgtc taaactaaaa gaggtatata atagcaagga
      301 tcctaaatat aagaaaattg acaagtattt acaaagttca ttatttaacg gttcagtagc
      361 tatatatgaa aatggcaaat tgaaaatgag taaaggttat ggatatcaag attttgaaaa
      421 aggtattaaa aacacaccga atacgatgtt tttaataggt tcagctcaaa aattttcaac
      481 agggttactg ttaaaacagt tagaagaaga acataaaata aatatcaatg atccagtaag
      541 taaatacctt ccatggttta aaacatctaa gcctatccca ttgaaagatt taatgttgca
      601 tcaaagtgga ttatataaat ataaatcctc aaaagattat aaaaatttag atcaagcagt
      661 taaagcgatt caaaaacgtg gtattgatcc taagaaatac aaaaagcata tgtataacga
      721 tgggaattat ttagtacttg cgaaagtaat tgaagaagtt acaggtaaat cttatgctga
      781 gaattattat acaaaaatag gagatccttt aaaacttcag cacacagcat tttatgatga
      841 acaacctttt aaaaaatatc tagcaaaagg ttatgcttat aatagtacag gactttcatt
      901 cttaagacct aatattttg
//
LOCUS       DAMZLE010000286          896 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMZLE010000286 DAMZLE010000000
VERSION     DAMZLE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.292"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(132..272)
                     /locus_tag="POB92_002816"
     CDS             complement(132..272)
                     /locus_tag="POB92_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDI6580234.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
     gene            complement(276..839)
                     /locus_tag="POB92_002817"
     CDS             complement(276..839)
                     /locus_tag="POB92_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDI6580235.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALISVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
BASE COUNT          320 a          136 c          118 g          322 t
ORIGIN      
        1 tctgattttt tgtatcttat tccactaata aatgcagata taaagaacat taatgaaact
       61 tgaacaatta caaataaaac gtaattataa tcatttaatg cttccactta ctatcacact
      121 ttctcttata gttattcgtg taattgagtt aattcttttg gtacttcagc ttcgtccaat
      181 aaaaaatcac aatttatata cgcggctctg taaccgatgc tgttgaaaaa gtcaactaaa
      241 agctcaataa ctttgttgag taattttttc ataatttagt cctcctttga atagtatatt
      301 ggtagtaatg taatagcttg tataataata cccagttgaa taaattgggt atatggttct
      361 tttacaaaaa gtgtgataat gaaaagaatt gtactaatta taattgaaaa atatcttttt
      421 tgctttacaa gcctcgctgg gataggcttc ttcttagtgg ctgcaggtgc atatttaatc
      481 actactccaa cacttattaa tgctaaaaac atcattagtg tttcattaat atgaaaatgc
      541 aatactaata gaggtagcac aataaaaagt gtaatgcttt ctatataaca ccaaaaagat
      601 gaaggtgcat gtgcaccatg tgcatacctt ctaattagat aaaacgatat attagtaatt
      661 aaagtgaaga taaaaatgtt taaaatatag gcaatagtat acataacaat taatttacct
      721 atatttttag ctaagacctg catccctaat cgtacttgca aaaattgaat atgatctaag
      781 ttatttctct tttgaagata cgtggcaaac tggtcaattt tattatcaaa ataattcaat
      841 tttacaccac tctcctcact gtcattatac gatttagtac aatcttttat cattat
//
LOCUS       DAMZLE010000287          887 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMZLE010000287 DAMZLE010000000
VERSION     DAMZLE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.293"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..886
                     /locus_tag="POB92_002818"
     CDS             <1..886
                     /locus_tag="POB92_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6580236.1"
                     /translation="NINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKSTLIDILIG
                     NVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLEEIIE
                     LTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYFWSII
                     ENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITLSDEY
                     IRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVDSYFN
                     NKKQRGSNYDTKLLENRI"
BASE COUNT          361 a          114 c          126 g          286 t
ORIGIN      
        1 taatatcaac aagtcattta ataaaagatg tgttctaaaa aatatttcgt tcgatattga
       61 acaaggtaaa tgtatcgctt taattggaaa aaatggtgct ggaaagtcaa cgttaattga
      121 tatattaatt ggtaatgtta atgctaattc tggtgagata tttgataaag acaagttatt
      181 acaaagtgaa aatcgcagta taatgttcca aaaaacgatg tttccagatc aattaaaagt
      241 tattgagatt atcaacttat atcaatcatt ttacgaaaat ccattaccat tggaagaaat
      301 aatagaactg acgaaatttg attctagtca actgaaccaa tttgtaaata aactttctgg
      361 tggtcaacaa cgattactcg attttgtatt atctttaatc ggacaaccac aattgatctt
      421 attagatgaa ccaacatcga ctatggatat agaaattaga gaatattttt ggtcaattat
      481 tgaaaattta aaagaagata atcgaacgat actctataca tcgcactata ttgaagaagt
      541 cgaacgtatg tcagacaaaa ttattctcat tgaaaatgga gaaataatac ttaatgattc
      601 aacgtcacat attagaacca atcagcaatc tcagattacg ttatccgatg aatatataag
      661 aaagttaaaa ctagataaag atgatttagt tattcaaaaa aatcataatg gcactatcaa
      721 aattattact tcaaatgtaa atgatacgat tttatatctt caacaacttc atattaattt
      781 ggatgatatt gaaatacaaa aagtctcaat tgttgattca tacttcaaca ataaaaagca
      841 aaggggatct aattatgata ctaagttact tgaaaatcga atttaaa
//
LOCUS       DAMZLE010000288          856 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMZLE010000288 DAMZLE010000000
VERSION     DAMZLE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.294"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            24..710
                     /locus_tag="POB92_002819"
     CDS             24..710
                     /locus_tag="POB92_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6580237.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEHPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          330 a          112 c          166 g          248 t
ORIGIN      
        1 ctaaaaagaa agaggtgtta gttatgacag aatacttatt aagtgctggc atatgtatgg
       61 caattgtttc aatattactt atagggatgg ctatcagtaa tgtttcgaaa gggcaatacg
      121 caaagaggtt tttctttttc gctactagtt gcttagtgtt aactttagtt gtagtttcaa
      181 gtctaagtag ctcagcaaat gcatcacaaa cagataacgg cgtaaataga agtggttctg
      241 aacatccaac agtatatagt gcaacttcaa ctaaaaaatt acataaagaa cctgcgacat
      301 taattaaagc gattgatggt gatacggtta aattaatgta caaaggtcaa ccaatgacat
      361 ttagactatt attggtggat acacctgaaa caaagcatcc taaaaaaggt gtagagaaat
      421 atggtcctga agcaagtgca tttacgaaaa aaatggtaga aaatgcaaag aaaattgaag
      481 tcgagtttga caaaggccaa agaactgata aatatggacg tggcttagcg tatatttatg
      541 ctgatggaaa aatggtaaac gaagctttag ttcgtcaagg cttggctaaa gttgcttatg
      601 tttataaacc taacaataca catgaacaac ttttaagaaa aagtgaagca caagcgaaaa
      661 aagagaaatt aaatatttgg agcgaagaca acgctgattc aggtcaataa tgctcattgt
      721 aaaagtgtca ctgctgctag tggcactttt ataatttttg gatcacgata tgatttatta
      781 tcaattcaga attaaaaaag taaatagtat caaaagcaag tgtatttaat attagaaaat
      841 aaaaatttta aattta
//
LOCUS       DAMZLE010000289          849 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMZLE010000289 DAMZLE010000000
VERSION     DAMZLE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.295"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            182..>849
                     /locus_tag="POB92_002820"
     CDS             182..>849
                     /locus_tag="POB92_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDI6580238.1"
                     /translation="MNNCFGEFEVKYLTGFILILLLEEILTNSASAIEYSDLHHKSKF
                     DSKRLSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSK
                     KFINKDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQA
                     EIELTAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEQNIEYN
                     FYNL"
BASE COUNT          319 a          106 c          151 g          273 t
ORIGIN      
        1 cacattatcg taagctgact ttccgccagc ttctatgttg gggcccctga cttgaattga
       61 aaaaaacttg ttacaagcgc attttcgttc agtcaactac tgccaatata acttcgtaga
      121 gcatagaaca ttgatttatt tcccagcctg tttatcattt atgtctcgct ctcaaatgta
      181 aattaataat tgttttgggg agtttgaagt taaatattta acaggattta ttttaatatt
      241 attgttagaa gaaattttga caaattcagc aagtgcaatc gaatattcag acttacatca
      301 taaaagtaag tttgattcaa agcgtctaag taatgctaag atgtcattca tcaatccaac
      361 tcagcttgaa aataaaaaca caaacgatag actgttgaag catgatttgt tatttcatga
      421 catgttcgta aatgatgatt ggaaaaaaga ttttaaagtt gaattcgaaa atgaggcact
      481 ttcaaagaaa tttataaata aggatatcga tatatttgct ggaaattatg gatatggatg
      541 tcatggggga gcaaccaata aaacgcaatg tagttatggt ggtgttactt taagtgacaa
      601 taataagtac gatgattata agaatatacc ttgtaattta tggattgacg gacatcaagc
      661 agaaatagaa ttaacagcag taaaaacgaa aaagaaaatt gttaccattc aagaattaga
      721 tgttcaatta agaaattatt tgaatgagaa gtataagttg tacgaacaag gtggcgacat
      781 tgttaaaggg tatgttaaat atcataatga tgatgaacaa aatatagaat ataattttta
      841 taatttaaa
//
LOCUS       DAMZLE010000290          841 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMZLE010000290 DAMZLE010000000
VERSION     DAMZLE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.296"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..841
                     /locus_tag="POB92_002821"
                     /pseudo
     CDS             1..841
                     /locus_tag="POB92_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          337 a           94 c          130 g          280 t
ORIGIN      
        1 ttggaactgg aagaagagtt tgaagaaaag aaaaaatact taagtgaaat ttatgaattg
       61 atagacgaaa ttaatattga cccttatatt tttctgacag aacataatga atgtaatata
      121 tacaaaataa gtgagaatga aaaattagaa cttcaaactt atatgagtaa aatcgaaaaa
      181 gtattattaa aatatcaaac gtatttaaaa gaaacatgta aagaatgctt gttttatcct
      241 tatttgctag ttcaaggtga agctggcata ggaaaatcac accttttagc gcatctatct
      301 aaaaaactta gagatgaaaa tcatattatt tatctgtttt tgggacaatt ttttactaaa
      361 aatgaagatc catggcatca aatacttaat gatttagaag ttacaaattc tgttgataat
      421 tttttaaggt caataagtaa taaggccaaa gaaacaaaaa aagagctttt attattattg
      481 atgcgcttaa tgaaggtgaa ggtaaaaggt tatggggaaa ttattttcaa agctttatca
      541 accatataaa gaagtattct aatatagctt taatattttc tattagaacc ccttttgaag
      601 atgttatatt acccaaaaac gcaatacaag ataacaatat tgtagtattt cagcatgaag
      661 gttttagtaa ggaagaaaac tataatccaa ttgtatcttt ttgtgatttt tatggattag
      721 agctacctaa gttacctata ttaaatccag aattcaacaa tccattattt ttaaaactaa
      781 tgtgtgagta ctgtgtaaat aaatttaagg aatttgacca aactataagt gttgcagagc
      841 t
//
LOCUS       DAMZLE010000291          827 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMZLE010000291 DAMZLE010000000
VERSION     DAMZLE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.297"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..404)
                     /locus_tag="POB92_002822"
     CDS             complement(<1..404)
                     /locus_tag="POB92_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HDI6580239.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFD"
     gene            495..>827
                     /locus_tag="POB92_002823"
     CDS             495..>827
                     /locus_tag="POB92_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6580240.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMS"
BASE COUNT          319 a          108 c          114 g          286 t
ORIGIN      
        1 ttatcgaaga ttctaaacaa ttttttgatt tctatttctt ttagagataa agtagggaaa
       61 atgtcgatat attcttcaag cgcataaaga ctatgtttaa cagaatcggt tatgttcatt
      121 gaatatatca caccaatatt tatattttgc catccaaaag aagaaataac attatcataa
      181 aatccattga atataaattc agtcatcccc tctgaacttc tccataaata taatggtgaa
      241 taagtatttg taatgcaatt agtagttgtt tgacttatta aataagcttt tataattaaa
      301 tttttaaatc catcagtctt ataaccattt aatcgaactc tatctctaat tatttccata
      361 ttgtaatcac taggcaactt aacatgatat tgcattgcat gcatttagca tacccccttt
      421 tataaaaagg atagcaataa taagtaaaat ctcatattat ccaattgtga tatagttatc
      481 ataaaaagtg ataggtgatt aaattgaact ttaatgattt ggaaattttt ataactgtat
      541 gtgaagaagc atctatcaat aaagctgcaa ttaaacttag atatgcacaa tctaatatat
      601 ctcaaagaat tagcaagctt gaaaatgaat taggtgtagt tttgcttttt agaaatcaaa
      661 aaggtgctaa ggcaactaaa gcaggcgaag aattcttagc gtatagcaaa aaagtattaa
      721 gagatacaga gactataaaa aataaaatga aaaataatac tatgtctatt ttatgctcag
      781 aactgttatt taattattta tctgagagcg aagaaattat gatgtcg
//
LOCUS       DAMZLE010000292          825 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMZLE010000292 DAMZLE010000000
VERSION     DAMZLE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.298"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            67..666
                     /locus_tag="POB92_002824"
     CDS             67..666
                     /locus_tag="POB92_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI6580241.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT          312 a          122 c          149 g          242 t
ORIGIN      
        1 attattgcaa aacacacccg aaattaatta ttataaaagt atattcataa aaggaggaat
       61 atacttatgg catttaaatt accaaattta ccatatgcat atgatgcatt ggaaccatat
      121 atagatcaaa gaacaatgga gtttcatcac gacaaacatc acaatacgta cgtgacgaaa
      181 ttaaacgcaa cagttgaagg aacagagtta gagcatcaat cattagcgga tatgattgct
      241 aacttagaca aggtaccgga agcgatgagg atgtcagtcc gtaataatgg cggtggtcat
      301 tttaaccatt cattattctg ggaaatacta tcacctaatt ctgaagaaaa aggtggcgta
      361 atagatgaca tcaaagcgca gtggggcact ttagatgaat ttaaaaatga atttgcaaat
      421 aaagcaacaa cattatttgg atcaggttgg acttggttag ttgttaatga tggcaaatta
      481 gaaattgtga caacgccaaa ccaagataat ccattaacag aaggcaaaac accaatctta
      541 ctatttgatg tttgggagca tgcctactat ctgaaatatc aaaataaacg tccagactat
      601 atgactgcat tttggaatat tgttaactgg aaaaaagttg atgaattata ccaagcagca
      661 aaataatata acttaatata attgaggtgg agcatctaca aggtgttcca cttttttgta
      721 cttaatatct ttacttattt gtttttgtgt aatgggatag cacgtaaaag tggcaaaata
      781 ataagataaa tttcaactcg aaatacctta ttattgttga ctata
//
LOCUS       DAMZLE010000293          817 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMZLE010000293 DAMZLE010000000
VERSION     DAMZLE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.299"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            46..>817
                     /locus_tag="POB92_002825"
     CDS             46..>817
                     /locus_tag="POB92_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6580242.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQA"
BASE COUNT          320 a          121 c          103 g          273 t
ORIGIN      
        1 aatattatct aatgaattat taactttttt gaaagaggta agttcatgaa tttagattgg
       61 tactatacat ttgtcatttt agcgaagacg ttaaactatc gattagctag tgaagaaatt
      121 aatctcacta taccttctat acataagcaa attaaaaatt tagaacagca tctcaatgtg
      181 aaactatttg aaacttataa aaatcaaatt atcttgactg aagatggtca tacatttctt
      241 ccaattgcgc aaagttttat tgaacaatat gaaagtggca tcaaacatat ccaacttaaa
      301 aaaacgatgt ttcaatcgaa attaaatgtt gtcgtgtctt catatatcgc gacattcatt
      361 atgcccaaat ttttaaaatc tttctttaac gagcatcctt ttatcgatgt ttctcttcat
      421 gtcaaaaatg aaaatattga aaaagatatt aataatcata cgtatgacat cgggattagc
      481 agaaatcaac ctaaactaag agaagttcat tctgaaaaag tatgcgaagg taaaattgta
      541 ttgattgctc ccaataaaga gaataatcat cttctaactg aggcatcttt atttgaaaaa
      601 tacaaaataa taagtgataa ccatccagaa tattggtcat cacttaaaaa taatatttta
      661 aatatttatg aaaaagcaca atttctatct atcaatgatg tgcatacttc aattaaatta
      721 atcgaaatga atcaaggtat atcattcctg cctctttata taacaacaaa tagtgattac
      781 aatatttcgg ttattaacac gaaaatttta caagcac
//
LOCUS       DAMZLE010000294          786 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMZLE010000294 DAMZLE010000000
VERSION     DAMZLE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.300"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(602..775)
                     /locus_tag="POB92_002826"
     CDS             complement(602..775)
                     /locus_tag="POB92_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580243.1"
                     /translation="MNKKATKLLEDYKKVQQRKSEETDRGCLKSVIMLIVLFFLIIFG
                     IVACSTNVNFFFQ"
BASE COUNT          275 a          137 c          132 g          242 t
ORIGIN      
        1 caattattta ctttgctctc ctatttttta aaatatgtac atgtttaaac aatcaaaagt
       61 gtacaatatt aaattatcat ttccagttct agtgctatat tggtagtagt tgactaaatg
      121 aaaataagct tataacaagt tttttcaata ctcgtggggc cacaacagag agaaatagga
      181 tcaccaattc caacagacaa tgcaagttgg cggggcccca acatagagaa attggatcac
      241 caatttcaac agacaatgca agttggggtg gggcccaaca cagaagctgg cgaaaagtca
      301 gcatacaaaa atgtgcaagt tggcggggcc acaacagaga gaaataggat caccaattcc
      361 aacaaacaat gcaagttggc ggggccccaa catagagaaa ttgggtcacc aatttcaaca
      421 gacaatgcaa gttggggtag gacatcgata aaaaaatact ttttctttag aaattagtat
      481 ttcttatgca tgagttttac tcatgtattc attttttaag tagacattag ctacagctaa
      541 tgataaagaa ccactacata ataaatcatt agtggttctt tatcatttct atctcactct
      601 tttactggaa gaaaaagttt acgtttgtag aacatgccac aataccaaaa ataattaaga
      661 aaaataagac gataagcatg atgacacttt tcaaacaacc tctatcagtt tcttccgatt
      721 ttctttgttg aaccttttta taatcttcaa gtagttttgt cgctttttta tttatatgtt
      781 tattca
//
LOCUS       DAMZLE010000295          780 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMZLE010000295 DAMZLE010000000
VERSION     DAMZLE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.301"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            57..>780
                     /locus_tag="POB92_002827"
     CDS             57..>780
                     /locus_tag="POB92_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI6580244.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLGVATQQIDKLIENIPLL"
BASE COUNT          321 a           91 c          141 g          227 t
ORIGIN      
        1 aatctaaaaa gataaaaaat attttagaaa acgaattgag aaaggaggat taatgaatga
       61 aaccattaca tttaaagttg aataatttcg gccccttttt aaaagaagaa attgattttt
      121 ctaaaattga taataatgaa ttgtttttaa taagtggtaa gactggatcg ggtaaaacaa
      181 tgatttttga tgcaatgact tatgccttgt ttggtaaagc atcaactgaa caaagagaag
      241 aaaatgattt gagaagtcat ttcgctgatg gtaaacagcc gatgtcagta acatttgaat
      301 ttcaattaaa tcatcgaatt tataaagtgc atagacaagg cccttatatc aaagaaggta
      361 atacaacaaa aacgaacgct aaatttgatg tatttgagat ggtggatggc aagtatgaaa
      421 ttagagaaag taaagtaatt tcaggtaccc aattcattat tgaattatta ggagtaaatg
      481 cagatcaatt ccgacaattg tttattttgc ctcaaggtga attcaaacgc tttttaatat
      541 caaacagtcg tgaaaagcaa gggatattaa gaacactgtt tgacagtgaa aaatttgaag
      601 ctatacgaga aatattaaaa gaagaagtaa aaaaagaaaa agctcaaatc gagaatagat
      661 atcaacaaat tgacctttta tggcaagaaa ttgaatcatt tgatgatgac aatataaaag
      721 gcttattagg ggttgccact caacagatag acaaattgat tgaaaatata ccacttttac
//
LOCUS       DAMZLE010000296          768 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMZLE010000296 DAMZLE010000000
VERSION     DAMZLE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.302"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            124..579
                     /locus_tag="POB92_002828"
     CDS             124..579
                     /locus_tag="POB92_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580245.1"
                     /translation="MAKLDLNSLNDEHVKLLINELKYPETHIDVNELKTIVASRINER
                     QEIISFKLGIKYLLTIKRGNIEKDRFSISIIFKDTYHTLVRIDINGGTHDNPDGTIAP
                     KSHIHIYNDKYDKKDRFAYEINLKDFPDIYNLYNVYMSFLEYNNIKDLE"
     gene            592..>768
                     /locus_tag="POB92_002829"
     CDS             592..>768
                     /locus_tag="POB92_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580246.1"
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDN
                     LIIYALYDQSRDMIT"
BASE COUNT          313 a           94 c          116 g          245 t
ORIGIN      
        1 gagatgctaa attgataata tacacttttg gttgtgtata catctcacct atcttttttt
       61 gcttttttca aataagaaag tattaaaaat taatgcctat acaatgttaa aggaggaaca
      121 gacatggcta aacttgattt aaatagtctt aacgacgagc acgtaaaatt attaataaat
      181 gaattaaaat atccagaaac tcatatcgat gtaaatgaat taaaaacaat agttgctagt
      241 cgaataaatg aaaggcaaga aataataagt tttaagttag gaataaagta cttattaacg
      301 ataaaaagag ggaacataga aaaagatagg ttttcaattt caatcatttt caaagatacc
      361 tatcacaccc tagttagaat agatattaac ggtggtactc acgataatcc agatggaaca
      421 atcgctccga aaagtcatat tcacatatat aatgataagt atgataaaaa ggataggttt
      481 gcttatgaaa ttaacttgaa agatttcccc gacatctata acttgtataa tgtgtatatg
      541 tcgtttttag agtataataa tataaaagac cttgaataaa ggagggataa tatgactaca
      601 tttgatgcta aaaaattaaa aaaagaatat cttgattggt ataatcagac cttagagttt
      661 tctaatttat caaacaatgt agtaagaata gatactcctt ttaaagataa ttctttagat
      721 aatttaataa tttacgcttt atacgatcag tccagagaca tgattaca
//
LOCUS       DAMZLE010000297          767 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMZLE010000297 DAMZLE010000000
VERSION     DAMZLE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.303"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>767)
                     /locus_tag="POB92_002830"
     rRNA            complement(<1..>767)
                     /locus_tag="POB92_002830"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          159 a          232 c          166 g          210 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actagctaat gcagcgc
//
LOCUS       DAMZLE010000298          763 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMZLE010000298 DAMZLE010000000
VERSION     DAMZLE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.304"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(32..>763)
                     /locus_tag="POB92_002831"
     CDS             complement(32..>763)
                     /locus_tag="POB92_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6580247.1"
                     /translation="LNFKEIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELL
                     QQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLY
                     PYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSN
                     DHNLFFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDN
                     KMNNQMNRIELLKERKKELLQKMFI"
BASE COUNT          216 a          121 c          103 g          323 t
ORIGIN      
        1 tatcatttca attaataggt tgttatcagg gttaaataaa cattttttgt aatagttctt
       61 ttttacgttc ttttaataac tcaattctat tcatttgatt attcatttta ttgtctatag
      121 attttaacaa tgcacttact ttatcttgtt cagtgagaca aggtatttta agatttatat
      181 tgcataagtt cgcatgagtt aatttagctg gtgcattacc tgtgacaaat gctcgtagtt
      241 ctttaaaatt taaataataa ttcataaaaa acaaattatg atcattactt ttaactacat
      301 gcgcatgatt atttacccag tattgcccat tagcaataaa gctactcgtc tcaaactgcc
      361 cccattttgc accatcttct cctattagta atcgttcttc attattgaat aaataatctt
      421 ttacgtaatc aataattcca gttgcaccat agtaagggta taaccccttt tctcttaatg
      481 aagaagtaat tggttttctt ctattatttt caaagataaa gatgtctttt atgaatttgt
      541 tttcccactc tggatattct tcaccattct catctttgaa tcgcaattct tgtgtgaaga
      601 ttttctgcat atagcctttt ttctgttgtt gaagcaattc aagtttttgt tcttctaatt
      661 caatttgtcg gtcgagtttg ctgaagaact tgcctatttt ttgctgttct tcaaatatag
      721 ttggggtgaa gatttttaaa ttagcaattt ctttgaagtt tag
//
LOCUS       DAMZLE010000299          749 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMZLE010000299 DAMZLE010000000
VERSION     DAMZLE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.305"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            278..749
                     /locus_tag="POB92_002832"
                     /pseudo
     CDS             278..749
                     /locus_tag="POB92_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
BASE COUNT          270 a          117 c          114 g          248 t
ORIGIN      
        1 taatcctaat aatagaggta ttgataaaat agcatgtgtt aaataaaaag ttaatagtaa
       61 ctacaaacat actttactaa taatcaaaac gacttataca aactcgaagt tatataagtc
      121 cccttatgga atctcacact ctagtttaca taatatatat tataggaagt tatgtgtgtg
      181 taacgcaaaa ggtaccctac atcataatca ttatctaata tcgtcacata acttacttat
      241 gctataatca tggtattata ttgtttggag tgatttgatg agatttgtct ttgatattga
      301 tggtacgctt tgttttgacg gccgattaat tgaccagact attattgata cattgttaca
      361 attacaacat gatggtcatg aacttatatt tgcatcagca cacattaatt ggcgcaaatg
      421 gtgctatgat ttcacagcaa tcaaagattt ctgttatcaa accaattcat acagatacat
      481 atcaccatat cttaaaaata attcaaaagt atgagttaga ttatattatt gatgatgatt
      541 ggaattatgc tgcacaactt gacgctgaga acgcgatttt tgagcgttta gatccacata
      601 agctggccag ttgtattgat gttgcaaata tcgacacgcc aatcaaagtt attttattaa
      661 atatagaccc ggcacaaatt acaactatat tagacgagct aaataaatac catcaagaat
      721 tggaaatgat tcaccattca aatgagtat
//
LOCUS       DAMZLE010000300          727 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMZLE010000300 DAMZLE010000000
VERSION     DAMZLE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.306"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..597)
                     /locus_tag="POB92_002833"
     CDS             complement(<1..597)
                     /locus_tag="POB92_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6580248.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLL"
BASE COUNT          292 a          113 c           93 g          229 t
ORIGIN      
        1 caacaaatta ataaatagat taaatcccat ttctcttggt gaatagtcac ctctttttaa
       61 acgattaaaa tcatacaaag tataaagtag aaataacaac aaactaacga cggtaataat
      121 agtgtaaaaa ataggatttt gaagaaaaat accaattaga ctcgcaataa ttaatgttat
      181 taatgtaacg aataaatatt tgcctatact cgatgcatct ccaacgaaga aataaccaat
      241 aataccaaat gctataaatg caaaaattgc taatgcgata tttttataga aaatatctgg
      301 tcctaaattt tgtaaatacg tggtaaacgt tgcgtatgac aataagccga tcactacagc
      361 gtaaatatgt gaaataatta aaccgaaccg tctcgctctt tcaaaaacaa gtgttgctaa
      421 aataataatc agtaatccaa atgacaaggg ttgtcgccaa cttaatggta aaaactgacc
      481 tagatagcat cctatgccaa atataatcca ataatacata aaataaagcc acactttact
      541 tattgattga gcatgctggt tttgatgata atatgaatga tttgtatttt gcgacaaaat
      601 aattcctcct tctaccgtcg ttttatgcgt caaattttac agtaaatttt gcttcatcaa
      661 aagaaataac ctttaataag tatagtaatt ttacatttta caatgttaca aaatataatt
      721 ttttata
//
LOCUS       DAMZLE010000301          727 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMZLE010000301 DAMZLE010000000
VERSION     DAMZLE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.307"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>727
                     /locus_tag="POB92_002834"
     CDS             <1..>727
                     /locus_tag="POB92_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580249.1"
                     /translation="LIYKEKQDSYVKKDISSINKSQYFKKLVIEGLIRERKSNSQYFE
                     IANSIKPSSHNNNSLNQSDEIDQFRQAVIGISLNLYDHNDTYFKFIRSIMKKSSRKVN
                     LTIDDIIKSKNNIDKFMKKNKLISNNELFYLTTIEDTFHFTLFTAITDLIDKVVNVSS
                     RIIEEASQYDILIALHSLNSLKNELNTIIDKHEENLIRFESLINGYVRNTQLKILVRN
                     TIEQHPELTMKIEDSLISNLNDYN"
BASE COUNT          312 a           86 c           88 g          241 t
ORIGIN      
        1 cctcatttat aaagaaaaac aagattcgta tgtaaaaaaa gatatctcaa gtataaataa
       61 atctcagtat tttaaaaagt tagtaatcga aggtttaatt agagaaagaa agagtaactc
      121 tcaatacttt gaaatagcta atagtatcaa accttcatct cacaataata atagcttaaa
      181 tcaatctgat gaaatagatc aatttagaca ggctgtaatt ggtatttctc ttaatctata
      241 cgatcataat gatacttact tcaaatttat ccgtagtatt atgaaaaaaa gttcacgtaa
      301 agttaattta acaatagacg atattattaa atctaaaaat aatatagata aatttatgaa
      361 gaaaaataag ttaattagta ataatgagtt attttattta acaacaattg aggatacatt
      421 tcattttaca ttattcactg ctataactga tttaattgat aaagtagtca atgtttcaag
      481 tcgtattatc gaagaagcat ctcaatacga tatattaatt gccttacaca gtttgaatag
      541 tttaaaaaat gagttgaata caataataga taaacacgaa gaaaatttaa ttcgtttcga
      601 aagtcttatt aatggttatg taagaaatac tcaattaaaa atattagttc gcaatacaat
      661 tgaacagcac ccagaactaa caatgaaaat agaagattca ttaatttcaa accttaatga
      721 ttataat
//
LOCUS       DAMZLE010000302          712 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMZLE010000302 DAMZLE010000000
VERSION     DAMZLE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.308"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..590)
                     /locus_tag="POB92_002835"
     CDS             complement(<1..590)
                     /locus_tag="POB92_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDI6580250.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMN"
BASE COUNT          275 a          105 c           76 g          256 t
ORIGIN      
        1 atattcataa acataaatag catagtcatg aaaagccaag aaacatataa gattaaattt
       61 tgcacatttt caaataccaa caagtaaaat gccccataag acatgaaacc agtaaatagg
      121 taataaaaaa ttgtgaaaca aatattttta aaggagggta ataatcttaa tttaagttca
      181 ttatttttac tttcattata aatatttaat gttaatttct tatttagata acaataaaaa
      241 aatataatct ctaaaaaccc aatgaaacag ctgctccaaa caattagttc ttctaattga
      301 aaatcaaata aataagtatt tcttcttaca gtaccaccaa gtaacacaat acccgcgaat
      361 atggtccatc ctatcgattt atttttaact ggtttgacaa tatttaattt ttcataatca
      421 ttcttagtga ttttgacata tgttttcggt atgagccagt taataaatgg aaagaagaag
      481 acaatccaat tacttgccaa atcaatcatt aaatattcac tatcgtattt gattattcga
      541 tatttagggt ttttattaat aactttagat tcgcaaagca atgtctccac atccctttaa
      601 ttttatgtgt aatacatttt tcgatacttc aaaagacatt caaatactat caagttactg
      661 tcatcaaagg ttttattaac tgatattttc atatttttaa tctgaattta gt
//
LOCUS       DAMZLE010000303          668 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMZLE010000303 DAMZLE010000000
VERSION     DAMZLE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.310"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            65..190
                     /locus_tag="POB92_002836"
     CDS             65..190
                     /locus_tag="POB92_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580251.1"
                     /translation="MIYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            452..>668
                     /locus_tag="POB92_002837"
     CDS             452..>668
                     /locus_tag="POB92_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580252.1"
                     /translation="MCFDINNLDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVV"
BASE COUNT          261 a           69 c          109 g          229 t
ORIGIN      
        1 tataattaga taattaatat gtcttggaat taattattta ataagttgta tagcaggtgg
       61 cgatatgata tatatacact tttctattat tacattaatt acaggtatca ttatgcatat
      121 tacaatgtac tttgtaccat ttgaatctcg gaaaatgcca ctatttatga cgataatttt
      181 tacaatctaa aagcagtaaa gactcataat ataaccaaaa acattaataa aagtttgtta
      241 aatgataagg ggatgagtat tggtaaattt actcaaaagg tttaagggaa aaatcctacc
      301 tggagagatc caaaaactaa atggactatt tccaaaaata aagggcaatc acatggtggt
      361 tcttattgga agttaataaa taacaaagga aaaagaatag cttctttaac taaagaagga
      421 aaaatcttaa gggaataagg tgttgtaaat tatgtgcttt gatataaata atttggatat
      481 aaaaaagttg aattttagaa aggtaaaaaa cgcaattcat ttaagtttaa gtggcgataa
      541 ataccagttt ttggaggacg gtaaaactat caataatact tattttttag ctgtatatga
      601 taatgcaata aacatttttg aagatttgtt ttcgccttct gattctataa atttggttca
      661 tgtagtat
//
LOCUS       DAMZLE010000304          656 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMZLE010000304 DAMZLE010000000
VERSION     DAMZLE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.311"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          285 a           85 c           92 g          194 t
ORIGIN      
        1 agaagtttag tgaatcgtct acatttagtt ggacaaattc tatgagaata gacattgtta
       61 aactaagagg gtaggcgatt ttattatgac aagagactga agagctttta gtaaataatt
      121 taaattaaaa taattaaact ttatgaaaat ggtaagctta gaaacgaaat tatacgagaa
      181 tatgatctaa catcattaac tttctcaaat tagataaaac aaaaccaaaa tactgagtca
      241 ttcaatcatc aagacatttt aacaagtgat gaaaaagagt taataaaatt acgcaaagaa
      301 aaacatcgat tgataatgta aaatgatgat ttaaagcaag cagtgctaat tttgagacaa
      361 aaaatgaagt ctttcaacaa aatgcgtatc aatattcagt atcagcaatg tgcaaagttc
      421 taaaaatttc aagaagtacc tattatgatt ctaaaaaagg agaatattac aactagatac
      481 aaaaagcctc aatcacaacc aagtaactaa aacgtaaaaa ttaattagat taattattat
      541 ctgcataaca aatgaccata tacatcaact agagacagac tatttaatca tttacaatgt
      601 cctattcgtt ttagaccatg ttcaaatgaa tagtgtttat aaaatgttga cactgt
//
LOCUS       DAMZLE010000305          649 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMZLE010000305 DAMZLE010000000
VERSION     DAMZLE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.312"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..629)
                     /locus_tag="POB92_002838"
     CDS             complement(<1..629)
                     /locus_tag="POB92_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580253.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRA"
BASE COUNT          245 a           99 c           66 g          239 t
ORIGIN      
        1 gctcttaaaa aagaataatg caatatccaa aacttctctt ctggtagaaa tttctttatc
       61 tttccctttg tttctcctat taaatttaat tccctatttt caactaatgt gcatcgaata
      121 attataattg ttagaattga aaatatacca gtcattatta taggccattt aatatttatt
      181 ccataaagat aactacctat aatccctgaa attaataatg ataggaacat atagctattt
      241 gacttatttt gtacgtattt ctcatttgtt atatttcttt taattattgc ggtatcaaca
      301 cccgccatca ttgagtaact taaccctaat aatatttgtg ccactactaa aattaaatat
      361 tgattttgat ataataacaa caataaaccg atgattttga atatttcact aacaactaat
      421 ttattagaat cttttaagtt acaaatttta aaacacttct ctttgtatag agagaataaa
      481 aatgctgcaa tgccataaga cgccattaat atttctaatt gtattatgga ataaccttga
      541 atcaaaaagt aaattaataa aaacggtaaa taaaagtatg ctcgtgaaaa aattcggtat
      601 aaataaatcc caagtattaa cttattcata aataatacct gtacctagt
//
LOCUS       DAMZLE010000306          649 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMZLE010000306 DAMZLE010000000
VERSION     DAMZLE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.313"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            153..235
                     /locus_tag="POB92_002839"
     tRNA            153..235
                     /locus_tag="POB92_002839"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:186..188,aa:Leu,seq:gag)
     gene            257..330
                     /locus_tag="POB92_002840"
     tRNA            257..330
                     /locus_tag="POB92_002840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Gly,seq:tcc)
     gene            449..>649
                     /locus_tag="POB92_002841"
     rRNA            449..>649
                     /locus_tag="POB92_002841"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          213 a          113 c          138 g          185 t
ORIGIN      
        1 taatgtttta ttataaatag cgttgtgtta acaactgata acacaaatta atttattgta
       61 aaaaaacgat taaaagttat tgacaaatat ttttaaacat aatataataa ataacgttct
      121 gaaaaacgaa gatgttttaa atgcctatat ccgcggtcgt ggcggaatgg cagacgcgct
      181 aggttgaggg cctagtggga gaaatcccgt ggaggttcaa gtcctctcgg ccgcatcaaa
      241 attcttaatt aaataagcgg gtgtagttta atggcaaaac ctcagccttc caagctgatg
      301 ttgtgggttc gattcccatc acccgctcca tagataattt taatgaacat tgaaaactga
      361 atgacaatat gtcaacgtta attccaaaaa cgtaactata agttacaaac attatttagt
      421 atttatgagc taatcaaaca tcataatttt tatggagagt ttgatcctgg ctcaggatga
      481 acgctggcgg cgtgcctaat acatgcaagt cgagcgaacg gacgagaagc ttgcttctct
      541 gatgttagcg gcggacgggt gagtaacacg tggataacct acctataaga ctgggataac
      601 tttgggaaac cggagctaat accggataat attttgaacc gcatggttc
//
LOCUS       DAMZLE010000307          634 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMZLE010000307 DAMZLE010000000
VERSION     DAMZLE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.314"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            30..>634
                     /locus_tag="POB92_002842"
     CDS             30..>634
                     /locus_tag="POB92_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580254.1"
                     /translation="MNRIEKHAKNTFIILMLIMLFWIFMSFIFQKLLFPPSKNNLTTY
                     EALKYYTHLKGYYGLDHISKGIAYIACVLIPFNFFFRFNDIKKDNNYNNIISTLFLLL
                     YFLVNGISLIIQGFTAEFTISLISESNIHNNHEFAVNLFRYVIQEGGISFSTYLVCNF
                     SIIMWLFFSCSLLKERKPVVRCLPLIISCLKLILILLFYFQ"
BASE COUNT          223 a           74 c           82 g          255 t
ORIGIN      
        1 gaaatgcaag taagtgaggt agttaaaaaa tgaatcgaat tgaaaaacat gccaaaaaca
       61 cttttattat tctgatgctc attatgttat tttggatttt tatgagtttt attttccaga
      121 aattattatt tccaccttcg aaaaataatt taactacata tgaagcatta aaatattata
      181 cacatttaaa gggatattac ggtttggatc atatatcaaa aggaatagca tacattgcct
      241 gtgtgttaat tccattcaat ttctttttta gattcaatga catcaaaaaa gataataact
      301 ataataatat tataagtacg ttattcctat tattatactt tttagttaat ggaatttcgt
      361 tgattattca aggattcact gcagaattta caattagttt aattagtgag tcaaatattc
      421 ataataatca tgaatttgct gtgaacttat ttagatatgt catacaagaa ggtggtattt
      481 cttttagtac atatctagtg tgtaactttt ctataataat gtggttattc ttttcgtgtt
      541 ctttattaaa agaacgaaaa ccagttgtca gatgtttacc attaattata tcttgtttaa
      601 aattaatatt aatcctactt ttttactttc aata
//
LOCUS       DAMZLE010000308          607 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMZLE010000308 DAMZLE010000000
VERSION     DAMZLE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.315"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..539
                     /locus_tag="POB92_002843"
     CDS             <1..539
                     /locus_tag="POB92_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6580255.1"
                     /translation="DSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSES
                     DSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLA
                     SIGSLLLFRRKKENKDKK"
BASE COUNT          212 a          111 c          127 g          157 t
ORIGIN      
        1 gtgactcaga ttccgacagt gactcagact cagacagtga ttcggattca gcgagtgatt
       61 cggattcaga tagtgattcc gactccgaca gtgactcgga ttcagatagc gactcagact
      121 cggatagcga ctcggattca gatagcgatt cggactcaga tagcgattca gaatcagaca
      181 gcgattcaga atcagacagc gattcagatt cagacagcga ctcagattca gatagtgact
      241 cggattcagc gagtgattca gactcaggta gtgactccga ttcatcaagt gattccgact
      301 cagaaagtga ttcaaatagc gattccgagt caggttctaa caataatgta gttccgccta
      361 attcacctaa aaatggtact aatgcttcta ataaaaatga ggctaaagat agtaaagaac
      421 cattaccaga tacaggttct gaagatgaag caaatacgtc actaatttgg ggattattag
      481 catcaatagg ttcattacta cttttcagaa gaaaaaaaga aaataaagat aagaaataag
      541 taataatgat attaaattaa tcatatgatt catgaagaag ccaccttaaa aggtggcttt
      601 tttactt
//
LOCUS       DAMZLE010000309          603 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMZLE010000309 DAMZLE010000000
VERSION     DAMZLE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.316"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(300..500)
                     /locus_tag="POB92_002844"
     CDS             complement(300..500)
                     /locus_tag="POB92_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6580256.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT          192 a          118 c           78 g          215 t
ORIGIN      
        1 taaacatttt gagtgtttaa cattacttct tgttatcgcc atatacagac tttaattctc
       61 gtcattatta aagttctgta ttggcgcttt tttatattca aaatcaaaag tcggacagat
      121 gaaaagtaaa aaacttttcc atcagttcga tctattttag aaccattata tattgttaaa
      181 tctccaccta aatttattgg acaaaaaaat aacggctagt gcttttacca caccaactgt
      241 tatcgattta aagcattaaa aaacagccct ataataagga ctgtttgaat aagttaaaat
      301 tacattttaa ctacgtttgc agcttgctcg ccacggtcgc cttcaacgat gtcgaattca
      361 actttttggc cttcttctaa tgatttgtat ccatcttcag cgattgctga gaagtgtacg
      421 aatacgtcgc taccatcttc tctttcgatg aaaccaaaac ctttttctgc attaaaccat
      481 ttaactgtac cgttattcat attgaatacc tccgtcgtgc ttttgcacta aatattttgt
      541 aacaaattca taaatgaaaa ggagaatatt ctatataaat aactcacatt tttcttcacg
      601 ctc
//
LOCUS       DAMZLE010000310          601 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMZLE010000310 DAMZLE010000000
VERSION     DAMZLE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.317"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            156..>601
                     /locus_tag="POB92_002845"
     CDS             156..>601
                     /locus_tag="POB92_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580257.1"
                     /translation="MNLRGHENRLKFLEKYDVTPISHLKLLEGQKKDGEGGRLTDSYY
                     CFSYSLKDNSKKVLGTFNCGYHIAEDLLKLSNQDKLPLFNPFKVINKGNQLQGVTNKG
                     NLDINRQRKQYNEVALQLSNAINLIIICYEGNIKEPLSTIKYETDK"
BASE COUNT          254 a           64 c           97 g          186 t
ORIGIN      
        1 ttactctaat agtacagaaa ctataaacaa caatcttcta aaatacgctg aaagttatca
       61 taagtgatgg taaaaaaatg agtaagtaga tgaagagtga aaatcagatt aattaataat
      121 aatgtatcaa atttaaataa agggggtctt taagtatgaa tttaagaggt catgaaaata
      181 gacttaaatt tcttgagaaa tatgatgtga cacctatatc acatttaaaa ttattagaag
      241 gtcaaaagaa agacggtgaa ggcggtagac tgacagatag ctattattgt ttttcataca
      301 gtttaaaaga taattctaaa aaagttttag gtacgtttaa ttgtggttat catattgctg
      361 aagatttact aaaattatca aatcaagaca aattaccttt atttaacccg tttaaagtaa
      421 ttaataaagg taatcaattg caaggcgtaa cgaataaagg taatttagat attaataggc
      481 aaagaaaaca gtataatgaa gtggctttac agctttcaaa cgctattaat ttaatcataa
      541 tttgttatga gggtaatatt aaagaaccac tttcaacgat aaaatacgaa accgataaat
      601 a
//
LOCUS       DAMZLE010000311          580 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMZLE010000311 DAMZLE010000000
VERSION     DAMZLE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.318"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            24..539
                     /locus_tag="POB92_002846"
     CDS             24..539
                     /locus_tag="POB92_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDI6580258.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          218 a           73 c          105 g          184 t
ORIGIN      
        1 ttattttaca aggagagagc aacatgaaaa taaaaactga atttaaaggg aacaatatac
       61 catatgaata cgcagcaggt gcagatgtga gtgattctat taacgggaat ccaattaagt
      121 catttccatt tgaagtaatt gaattaccgg aagggactaa atatcttgct tggtctttaa
      181 ttgactatga tgcaattcct gtatgtggct ttgcttggat tcattggagt gtagctaatg
      241 taagtgttag tggcaattca atttctataa aagcagattt atcaagaaca aagggtgact
      301 atgtacaagg taaaaatagc tttactagtg ggttgttggc tgaagatttt tcagaaatag
      361 aaaatcacta tgtaggacct acaccacctg atcaagatca tcaatatgaa ttaacagttt
      421 atgcgttaga tcattcttta aatttgaaga atgggttcta cttgaatgaa tttttaaaag
      481 aagtaaatca acataaaatt gatcaaacaa gtattaacct tataggaaga aaaatttaat
      541 actaaatatc tcatcaatat aaaattgttc aattaaaagt
//
LOCUS       DAMZLE010000312          571 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMZLE010000312 DAMZLE010000000
VERSION     DAMZLE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.319"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>571
                     /locus_tag="POB92_002847"
     CDS             <1..>571
                     /locus_tag="POB92_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580259.1"
                     /translation="DQDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYL
                     YDKQYVQFQAITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLL
                     LIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAM
                     IHNPQSAQIFSLIIWILLVVLVIYFTIRLS"
BASE COUNT          188 a           77 c           78 g          228 t
ORIGIN      
        1 aagatcaaga ttatattgaa tataaaagaa aatctccagc acaaatcata ttaatgattc
       61 aaaggaattt gtttgcttta caaatgaatc cagttatttt ctttattata aacttcattc
      121 tcatatccta tttatatgat aaacagtatg ttcagtttca agctattact ggaatgagtc
      181 tattttattg tttagtgatt tttccaatga ctattgttgt atacctaagg gtgtcacaaa
      241 agaattactt gcgtagtaat aaaatagaaa tgattatggg tacaattatc gctatcatat
      301 ccttgttatt aattatatta caagcattta atattacttg gggcgttata ccaattacaa
      361 attttggaca tcaatttttc tttttcattg gtattatttt agtaattgcc ggcatatttt
      421 ataagcgact tgagttttcg ggaatcggct tattattttg tcaaaaaacc gtcgatgcaa
      481 tgattcataa tccacaatca gcccagattt tttcattaat tatatggata ttattagtag
      541 ttctagttat atatttcaca attagattat c
//
LOCUS       DAMZLE010000313          560 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMZLE010000313 DAMZLE010000000
VERSION     DAMZLE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.320"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>560
                     /locus_tag="POB92_002848"
     CDS             <1..>560
                     /locus_tag="POB92_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6580260.1"
                     /translation="ASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYAR
                     QTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSID
                     KIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAK
                     LHGEFDMWVNRYDNFNNSIERISKKY"
BASE COUNT          223 a           53 c           99 g          185 t
ORIGIN      
        1 tgcttctgtt ttaggggcga cacttcgaat cgacatttgt gaaatacata tagagattgc
       61 taattatatt tatgaagaaa tatttgataa agaaatggta tatcctcata ttttaatgag
      121 agattatgct aggcaaacaa ttgaatatat tagtttatca aaagatatat ctaatataaa
      181 tttagaaaaa attaggccac cttataaaag taattggtat aaaaaagaat attcgaattt
      241 aaatatagac gattacataa aatctttaaa aaacaaatta gatagccatt tacatttttc
      301 tatcgataaa attaaaaact caatgacaac ggaatatgga agaggaactg gggcatatgg
      361 cgattttggc agatatgtat ttggttatgc tgtacgaaac tgggtaaagg gttttaaaag
      421 tgatcaagac ttaagtaata tagcattaat gaggattttt gaaatgggtt atgatgcaaa
      481 attacatggt gaatttgata tgtgggttaa tcgttatgat aactttaata attcaattga
      541 aagaattagc aaaaaatatc
//
LOCUS       DAMZLE010000314          550 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMZLE010000314 DAMZLE010000000
VERSION     DAMZLE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.321"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..214
                     /locus_tag="POB92_002849"
     CDS             71..214
                     /locus_tag="POB92_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDI6580261.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT          178 a           66 c          101 g          205 t
ORIGIN      
        1 tgtgtggtaa tgattaatat gacaattgaa gtgggtctta acttcaactt ttaaataggg
       61 ggttttatct ttggaaaaag ttcttgattt agacgtgcaa gttaaaggaa ataacaacac
      121 taatgattca gcgggtgacg aaagaataac tagccatctt ttttgtagct ttggttgtga
      181 aaagacgggt agttttaaca gcttctgttg ttaaggatac agcaaataga gcctgaaagg
      241 gctctatttg tctttttaaa tatattaagt gctaaaaaac ggaaacagtt tattaatcgc
      301 caggtatttt tataattagc cttctatatt atcagttgag tgtttcaata aattctgact
      361 ttggtgatgg aaagttatac ttttagttgt aatatagttt tcatgacttc ctgttaaata
      421 agttaggatt atgtaattag cctctatctt tacatataag taatgacaag ctttggttat
      481 tatattgacc ctcttagaac atgtcattta agaaaataat caagtcattg aaattatatg
      541 tttatatcct
//
LOCUS       DAMZLE010000315          534 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMZLE010000315 DAMZLE010000000
VERSION     DAMZLE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.322"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            51..301
                     /locus_tag="POB92_002850"
                     /pseudo
     CDS             51..301
                     /locus_tag="POB92_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          211 a           67 c           74 g          182 t
ORIGIN      
        1 ttaatcaatt ttgcatctat tttgttgtaa gctatataaa aggagtgata atgatggtga
       61 aaaaaacaaa atccaattca ctaaaaaaag ttgcaacact tgcattagca aatttattat
      121 tagttggtgc acttactgac aatagtgcca aagccgaatc taagaaagat gatactgatt
      181 tgaagttagt tagtcataac gtttatatgt tatcgaccgt tttgtatcca aactggagac
      241 ttttaacata aaattactta tcattcaaaa agtaaaacag cataatatca aggtttataa
      301 ctttatcatt atcaataata cctcatataa aataaaattt tagggacttt ttagggactt
      361 taaatttaaa attacaagtt taatagaaac atcaaaataa tcacatgttt gtgtggaatg
      421 tacaccccaa aagctagact gaaaaatcta ttttttgagg tgtattttta taggtaaata
      481 taataaatta gagtagacaa ctcagaattc caattttata ataatattgc ttga
//
LOCUS       DAMZLE010000316          502 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMZLE010000316 DAMZLE010000000
VERSION     DAMZLE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.323"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(281..469)
                     /locus_tag="POB92_002851"
     CDS             complement(281..469)
                     /locus_tag="POB92_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI6580262.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT          167 a           81 c           81 g          173 t
ORIGIN      
        1 ctgtaattaa tgaagattat ggcgtgattt attattgaaa ggaaaattgt aggtcctagt
       61 acattgattt atgtgccaaa atcgatattg aacccataag tccaatttga cagagaacca
      121 ttattaaacg tattaaccaa attatattga tagttcgaat ttcactatga accattatgt
      181 attgtttggt aacaaaaaaa gaactaagag gttgagctct tagttcttaa gtaaatgtgt
      241 cctccacata aggtcacatt ttttatgtgc aatatattta ttaaactcta gttactttac
      301 cagattttaa agcacgtgca gaaacccaaa cttttttagg tttaccgtca actaggattc
      361 taactttttg aaggttagcg ttccatctac gtttagtaga gtttaaagcg tgtgaacgtc
      421 tgttaccagt cgaagcttta cgacctgtta cgaaacattg tttacccata tgagtacctc
      481 ctttaataaa tataaataca ca
//
LOCUS       DAMZLE010000317          496 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMZLE010000317 DAMZLE010000000
VERSION     DAMZLE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.324"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..469)
                     /gene="sarU"
                     /locus_tag="POB92_002852"
     CDS             complement(<1..469)
                     /gene="sarU"
                     /locus_tag="POB92_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDI6580263.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFN"
BASE COUNT          159 a           70 c           58 g          209 t
ORIGIN      
        1 aattaaatcg atattttaaa aaatcttttg agtacataat gttcataaaa agtataaaaa
       61 attctttgct atattttgga gcccattgat attttatgta gtctctttcc ttattaaatc
      121 ctttaattat tttatctaac tcattaataa cacaagcaat ctttttacgt tgtattggag
      181 taactgaaac aaaaatacgg cgttcatctg cttcgtttct agacttattt ataaatcctt
      241 ttttagatag tgacttaatg ttctttacaa catcagattg tttgtaaagt atgtcaccaa
      301 tgatttcttt tagtgaaata ctgtgttcat ttttgtaata aaaatatgct aatatcaaca
      361 attcttttaa tgataattta tattgttgct ttaactcttg agtgagaaaa aatatgtacg
      421 cttctacatt tatgaattta ttgacctttt cgaaagtttg atagtccacc ttacattctc
      481 ctactatttt ctgatg
//
LOCUS       DAMZLE010000318          495 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMZLE010000318 DAMZLE010000000
VERSION     DAMZLE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.325"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            182..>495
                     /gene="sarT"
                     /locus_tag="POB92_002853"
     CDS             182..>495
                     /gene="sarT"
                     /locus_tag="POB92_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HDI6580264.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKELSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HN"
BASE COUNT          215 a           46 c           73 g          161 t
ORIGIN      
        1 ataacaaatg acaaattcat ttattatcca aaaataaaaa caattgaaca aaatcaaaat
       61 atagtgttat gattcatttg tgtttatagc tagcaccaca aactaaatat atttggagtt
      121 attactagtt aaagacaaat attatttgac gtgacaatta aaagtaaggg atgaagactt
      181 gatgaatgat ttgaaaagca agagcaatat taaattaatg aaaagagtat taactacata
      241 tgagctgcgt aaatatttaa aaaaatattt ttgtcttaca ttagataatt atttagtatt
      301 agcatattta gatgtattta aaaatgatga aggtaaatat tttatgagag acattataag
      361 ttatatcggt atagaccaat ctagaattgt taaaagcgta aaagaattat caaaaaaggg
      421 ttacttgaat aaatgtagag acccacatga tagtagaaat gtaatcattg ttgtttctgt
      481 aaaacaacac aatta
//
LOCUS       DAMZLE010000319          488 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMZLE010000319 DAMZLE010000000
VERSION     DAMZLE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.326"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            157..>488
                     /gene="sarR"
                     /locus_tag="POB92_002854"
     CDS             157..>488
                     /gene="sarR"
                     /locus_tag="POB92_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDI6580265.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELE"
BASE COUNT          205 a           53 c           71 g          159 t
ORIGIN      
        1 aaatgtattt aaccatattc tagtaataaa aatattgaat ttttatactt atttgtttag
       61 aatgaacttt ataacatagt tggatagagt ttcgatttaa taaattacat gtgaaccttg
      121 ctacaacaag atgtgcatca gaggagtggt ttaataatga gtaaaattaa tgacattaat
      181 gatttagtca acgcaacatt tcaagttaag aagtttttca gagatacaaa aaagaagttc
      241 aatttgaact atgaagaaat ttatatttta aatcatattt taagaagtga gtctaacgaa
      301 atctcatcta aagagattgc taagtgctca gagttcaaac cttactattt aactaaagct
      361 ttacaaaagc taaaagattt aaaattgtta tcaaagaaaa gaagtttaca agacgaaaga
      421 acagttattg tttatgttac agatacacaa aaagcaaata ttcaaaaact gatttcagaa
      481 ttagaaga
//
LOCUS       DAMZLE010000320          486 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMZLE010000320 DAMZLE010000000
VERSION     DAMZLE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.327"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..196
                     /locus_tag="POB92_002855"
     CDS             <1..196
                     /locus_tag="POB92_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580266.1"
                     /translation="LITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILF
                     FINIVLVLYYIYPIFYSFIG"
     gene            225..413
                     /locus_tag="POB92_002856"
     CDS             225..413
                     /locus_tag="POB92_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580267.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
BASE COUNT          145 a           68 c           68 g          205 t
ORIGIN      
        1 tttaatcaca tataataaag atttagatga tttatgttgg ctcctgcctg gtattatcat
       61 ttgtggtgtg atactcatcg tatcctttac cattgcaatg ataactaaaa actggttaag
      121 tgaaatatta ttttttataa atatcgtact cgttctctat tacatttatc ctatttttta
      181 tagttttata ggttaataat tacggtatat aaggagggtt tcacatgtct ttacattttg
      241 caattctgtt ttggctagca ttaattttct tagttgccgc tacgtttata ctcgtattaa
      301 tgaaaaaaac tggcaaagaa tctaaaaaag agtcctattt aagtttcact gtcattctct
      361 atatttttgg attcgctata ttaatataca catttatatt tggtgtgcta taaatgaaat
      421 tcattttata tccagtctga aacgtctcaa tgatgtttca gactgttttt tattcttaat
      481 agacat
//
LOCUS       DAMZLE010000321          485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMZLE010000321 DAMZLE010000000
VERSION     DAMZLE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.328"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>485
                     /locus_tag="POB92_002857"
     CDS             <1..>485
                     /locus_tag="POB92_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI6580268.1"
                     /translation="FSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHILMNMLS
                     LFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLIGSIFAM
                     MYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGYYYKVNR
                     N"
BASE COUNT          138 a           48 c           98 g          201 t
ORIGIN      
        1 ttttcggatg taaaattatt agatgttggc gggttagtgc attttaatgt cgtacatggt
       61 gaatggtatc gaattgttac atcgatgttt ttacatttta gttttgaaca tatacttatg
      121 aatatgcttt cattatttat ttttggtaaa atagtcgaag caattattgg ttcatggcgg
      181 atgttaactg tatactttat tgcagggttg tttggaaact ttgtatcact atcatttaat
      241 acgactacaa tttcagttgg ggctagtggt gctatatttg gtctgattgg atcaattttt
      301 gcgatgatgt atgtttcaaa aacatttaac aaaaaaatgt taggacagtt attaattgca
      361 ttagtgatat tagttggtgt ttctctgttt atgtcaaata taaatattgt ggcgcatatt
      421 ggaggattca ttggtggttt attaataact ttaattggct attactataa agtgaatcgt
      481 aatat
//
LOCUS       DAMZLE010000322          485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMZLE010000322 DAMZLE010000000
VERSION     DAMZLE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.329"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..293)
                     /locus_tag="POB92_002858"
     CDS             complement(<1..293)
                     /locus_tag="POB92_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDI6580269.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQ"
     gene            complement(283..429)
                     /locus_tag="POB92_002859"
     CDS             complement(283..429)
                     /locus_tag="POB92_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580270.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
BASE COUNT          173 a           78 c           50 g          184 t
ORIGIN      
        1 ttttgatttt tcaaaaaatt aaatgcctct tcactctcaa taataccacc accagtagca
       61 attatatctg cagtgttaat acattcttgc aaacatgtga actctaaatt cctgaaatat
      121 tgttcaccat gtttactaaa tatttctggt attgttaact tatacttctc ttcgatatat
      181 gaatctatat caataaatga taaattttgc tcatctgcaa cgtatttacc aatcgtagat
      241 ttaccggtac ccatgaaacc aattaaaatt attggtgatt tatcatgatt catgcgtttt
      301 cccttctttc aataacaaaa caatcttata gttatagtca gtttcaatat tatataatgt
      361 ctttaatttt aaactatatt gactgatata aaaagtgaaa atggaaaaat aaattgctgt
      421 tagcatcaat aagaacggcg taacatatcc tttttcttta tataaatcaa aattaataaa
      481 ttatt
//
LOCUS       DAMZLE010000323          479 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMZLE010000323 DAMZLE010000000
VERSION     DAMZLE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.330"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          207 a           56 c           75 g          141 t
ORIGIN      
        1 aatcagaacc tagatgatat aaataatctt tacgaattta ttgttaaaat aaaatagatc
       61 caaacagccc tgatctttag aactaatgat cagggttgtt atattttgta cctcactctt
      121 aatcgatacg ctgaaagtcc ctataaatag aacgtacgtt ccgtttgtag tataatgaca
      181 aaaatattaa attagaggat taaaaatggg attataattt tttaatgtac caaactgaaa
      241 aaggctaaat aaagataaat ataagattag aaaaaactgt atggattagg caatatttat
      301 atataatgaa atgaattaac agaaaatcga attatccgga aaaaccgtac tgttcgattg
      361 gaagggaata gagaaggtga aaagaattaa cttcaattat catcttattt ctgaaaaaag
      421 atatgaaaaa cggacgcaag acactttaaa tattagtaac aacgtaaagt gaataaaat
//
LOCUS       DAMZLE010000324          472 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMZLE010000324 DAMZLE010000000
VERSION     DAMZLE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.331"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          200 a           84 c           44 g          144 t
ORIGIN      
        1 agtaatgtta agtaaacatt ttatacttta tcagaacaaa aaagacatgc atcaacatgt
       61 cagtgaaact aatccatatt atcttaacaa cgaaattgaa tcgaacctga aaaacgtatt
      121 tacaaatcgc aactactcta tccaaggcaa aatcgcaaaa tctgatcccc gaaataacat
      181 atctcgaaaa aaattccata attcaatcat cttcaatatt taacaataca ttactagttc
      241 caaatatact catacataca tgtttcattg attgactgca tattgtcata aataataggg
      301 gaactaatcg ctaataatgt tgttaaacac aatacatagt aaagtgaaag attataaata
      361 catgaattgt tcaccattcc gccatatatt ttcaatcacc taatttccga ataaatatta
      421 caattttaat tcaataatta atatttaaga ccaaaataat cctaaaaagt ca
//
LOCUS       DAMZLE010000325          471 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMZLE010000325 DAMZLE010000000
VERSION     DAMZLE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.332"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          191 a           49 c           78 g          153 t
ORIGIN      
        1 gagtatatat ggagttcttt cttttttaca agatgtatat gtataaatgg aaatgtttat
       61 tacattacat aaaaaccttt aggtgctctt agatggatgt taatcataaa aaaagagatt
      121 ttgaaaaata taggctatta taatttagaa ttacaaatta taaatcttgc cagtggatat
      181 gggagttcaa catgtcactt accccaattt gtggaatgtt aaataactat aaagaggaaa
      241 ggagaaaaaa tgataagaat ggttaaagaa aaagaacgaa tagcaaaatt aaatttcgaa
      301 tagctgtgac gagcttaata attcaagtac tttattttat caaatcgttc ttttaaaaag
      361 gtaaagggca aaagaccttt accacattaa atagggaggt ggtatttatg acaagagaat
      421 taaggaaaaa attaactctc tatcttaaca ttgcaacttt aatattattt a
//
LOCUS       DAMZLE010000326          464 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMZLE010000326 DAMZLE010000000
VERSION     DAMZLE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.333"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..230
                     /locus_tag="POB92_002860"
     CDS             <1..230
                     /locus_tag="POB92_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580271.1"
                     /translation="EKIILIPTFKNICLSLFAYILFGGLSTMALSMLVTSSPQNIIEF
                     LALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            287..403
                     /locus_tag="POB92_002861"
     CDS             287..403
                     /locus_tag="POB92_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580272.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
BASE COUNT          182 a           61 c           66 g          155 t
ORIGIN      
        1 acgaaaagat catattaata cctacattta aaaatatttg tttatcctta tttgcttata
       61 tattatttgg tggattgtca acaatggctc tgagtatgtt agtaacttca tcccctcaaa
      121 atataataga atttcttgct ttaattggca tgactgcatg cttctttcta ctgaatatgt
      181 catcggttct agataaaaaa attcatgtta ttttaaaaac aaataagtag taaaattgat
      241 taacttaggt agtatcggat acttaaatgt tggttcataa aaagcaatga ttttaaatcg
      301 aggagctatc ttagaacagg gaaataaaac agccaaagtt ataaaaagtg aattaataac
      361 taattatatt atgttagcca cgcttcaaat aaaaaataat tagaataagg tgggattgat
      421 aatcaatgct atgcgaatct aaaatcatca ataaaaaccc caaa
//
LOCUS       DAMZLE010000327          454 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMZLE010000327 DAMZLE010000000
VERSION     DAMZLE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.334"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..454)
                     /locus_tag="POB92_002862"
     CDS             complement(<1..454)
                     /locus_tag="POB92_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580273.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEK"
BASE COUNT          177 a           73 c           62 g          142 t
ORIGIN      
        1 gcttttcttt atatataatt gcattaatac aataataaat aataaagagt gaaattagtg
       61 ttgatgtcgc ataattataa aatcctgcaa accagccata tgtatctgca taaatagcac
      121 ttggtaaaat taacattaaa gaaaatgcta acaaataata gcttgtccac gctttacaac
      181 gtgtaatgtg cataatcatc caaatgatgc ccatactaat gagaccatat gaaagccaac
      241 gtaaccagct tacatgtaca gctataattt caaagatatt tccgatatag cgaccattaa
      301 gtgatgcaaa tcctactttt aaaatatcag tattataatt gctaaaccat tgtaaatcat
      361 cgtgcatgag tggtagtaag atacccataa aagtataaaa caataatatc gcaattaata
      421 tcaaagttgt cttgtgtaat tgaattgttt tcac
//
LOCUS       DAMZLE010000328          427 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMZLE010000328 DAMZLE010000000
VERSION     DAMZLE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.335"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          159 a           70 c           46 g          152 t
ORIGIN      
        1 aatgttgtta aaatacaata tcttttccat ttacatctta tgtttaaaga tcaatagtac
       61 tacaactatt taaggataga tatacattct aagtttttaa atacattctt cagttatatt
      121 tgtatattcc aaatgtttat taatagcatt tctgatctca gaaatgccat taaggacaac
      181 gccctctttc ataactatat aagtcaagaa accaagaaat tccaatcatc tttaaacctt
      241 tcaatagcac atttcctaaa acataaaaca ataacaatga acgatcaaaa ttacttacgt
      301 gcgtatttaa ttatagattt gtcaatttca agtgatttag ccataactat aaattttcct
      361 attacaaaaa aaggccagct atgtttcgca ttgttaagag gcgttagctg acttctgata
      421 aattaat
//
LOCUS       DAMZLE010000329          422 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMZLE010000329 DAMZLE010000000
VERSION     DAMZLE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.336"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..387)
                     /locus_tag="POB92_002863"
     CDS             complement(<1..387)
                     /locus_tag="POB92_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDI6580274.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESI"
BASE COUNT          133 a           63 c           65 g          161 t
ORIGIN      
        1 gattgattca ttattaataa atgtcacttt acatttacga ttttttaatt ctttaatact
       61 ttcgatataa tgcatattta accaaatatt ttcattttgt ctgtcagaat gtgttgggaa
      121 aaaataagtt ggaaataatg gtgttagtaa aataggtggt ttactagaaa tgccagtaat
      181 gcggtttgtc tctgctttct tgccaagata agtattacca taaaatttgc aagagcgttc
      241 aatgattttc tggactttaa aaggattttg aatacgcgtt ttgtcaaacc gaattatttc
      301 actaccgttt ctttgatcgt catcatcaaa tgctggtcga ataaccatgt ctcctttgcg
      361 tatcacataa atattttgag aatacataag caaaacctcg ctttattaag ttttaaacca
      421 tt
//
LOCUS       DAMZLE010000330          419 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMZLE010000330 DAMZLE010000000
VERSION     DAMZLE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.337"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>419)
                     /locus_tag="POB92_002864"
     CDS             complement(<1..>419)
                     /locus_tag="POB92_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580275.1"
                     /translation="TNINFSLPISIKAITTIQLKHWISNKEEVNLHKPFFYISYSIWF
                     FLGCLIYFSQMAYNQLANAVIFILILNTITRDSFSAGTDFFTKSLRFDSDRKSIGLYR
                     MSNTDFKQIYDSKLSLIRFIGFKETILAIILLAIFLN"
BASE COUNT          166 a           54 c           54 g          145 t
ORIGIN      
        1 tgatttaaaa atattgctaa taaaataata gcaagtattg tttctttaaa accaataaag
       61 cgaattagag ataatttaga atcgtaaatt tgtttaaaat cagtgtttga catcctatac
      121 aaaccgatac tttttctatc tgaatcaaaa cgtaatgatt tagtaaaaaa atccgtcccc
      181 gcagaaaaac tatcacgagt tatagtgttt aatatcaaaa taaaaataac tgcattagct
      241 aactgattat atgccatctg tgaaaaatag attaaacacc ctaaaaagaa ccatattgaa
      301 taactaatat agaagaaagg tttatgtaaa ttaacttctt ctttgttact tatccagtgt
      361 tttagctgta ttgttgttat agcttttatt gaaattggca acgaaaaatt aatatttgt
//
LOCUS       DAMZLE010000331          412 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMZLE010000331 DAMZLE010000000
VERSION     DAMZLE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.338"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            164..>412
                     /locus_tag="POB92_002865"
     CDS             164..>412
                     /locus_tag="POB92_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6580276.1"
                     /translation="MLLYESNIINKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFINWL
                     IPKKYVQISREEFDNLNIVKPVKNKALWPVIGSILLFGT"
BASE COUNT          167 a           48 c           62 g          135 t
ORIGIN      
        1 aaaggtttat gccaaactta aaaagcaata gtaattacta aacttagatt gtagttcgta
       61 agttaagtaa taaacaggaa aatagtatgt tactaaaata caaaacactt ataaagtatc
      121 tgaaagtgta ttagatataa aaccaacgag acggaagtgg taaattttgc tttacgaatc
      181 taatattatt aacaaaaatc ccagatatag aattattaaa tataaaaacg actatctgat
      241 gattgattta gtaagcacat ggttagtttt gttctttccg tttattaatt ggttaatccc
      301 aaaaaaatat gtccaaatca gcagagaaga gtttgataat ttaaatattg ttaaaccagt
      361 taaaaataaa gctttatggc cagttatagg tagcatactt ttatttggca ct
//
LOCUS       DAMZLE010000332          411 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMZLE010000332 DAMZLE010000000
VERSION     DAMZLE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.339"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          166 a           48 c           58 g          139 t
ORIGIN      
        1 aggtgtacca cgtcaaacag ttactgattt aaaggtaggt aaaactaaaa taaaagaagc
       61 taaatttaaa acgataatca agttatatga atatcaaaga acattagaaa ataaaacaga
      121 atgttaacaa aaatattttg ttaggttagg tggacaagaa attaaatttc taatgatttc
      181 atggttgtct gtccacgttt tgtccacctc aataagataa tctcttagtt ttagaaattg
      241 tacatggtta aataatcaaa aatgcatatt atgaaattat cattgccatt aaatcttgaa
      301 tgatctttaa gcgatgatat aaaaggctga acacagtaaa taagttaact ttaatgcagt
      361 attctatact atacatattt tgtaataatg tttatgcaca taatttttca t
//
LOCUS       DAMZLE010000333          389 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMZLE010000333 DAMZLE010000000
VERSION     DAMZLE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.342"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          141 a           67 c           68 g          113 t
ORIGIN      
        1 aaatggtgtt ttaatattta aaattgaact tttgatatat tactatgtct ggtacacaaa
       61 tcaatgtttt atgctttaca aagttatatt ggcagtagtt aactgcagtc cacaaacata
      121 gaagctggtg gaaagtcagc ttactatttc atgcaagttg gcggagctcc aacatagtgg
      181 aattggattc ccaatttcta cagacattgc aaattgggga aacgggccac aacatagaaa
      241 aattggatcc tcaatttcta caaacaatgt aagttgggga aacagcccca acactgaaac
      301 tggcagaaag tcagcttcta tgaatataat aaaaaagcta ggtaacaaaa tgctacctaa
      361 cttcatattc aagataatca atcctattt
//
LOCUS       DAMZLE010000334          367 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMZLE010000334 DAMZLE010000000
VERSION     DAMZLE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.343"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>367
                     /locus_tag="POB92_002866"
     CDS             <1..>367
                     /locus_tag="POB92_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI6580277.1"
                     /translation="IETKIANSKKDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIE
                     SKISFIDKTRVLFNDINKYQQSYLNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNE
                     SDYEKIIELNNTITNINNEI"
BASE COUNT          172 a           33 c           59 g          103 t
ORIGIN      
        1 attgaaacta aaattgctaa tagcaaaaaa gatatttcta aaaccaataa taaaatattg
       61 aatcttgatt gtgataagcg aaacatagac aaagagaaaa aaatgttaga agaaaatgga
      121 gatttaattg aaagtaaaat ctcttttatt gataaaacta gagtattatt taacgatatt
      181 aataagtatc aacaaagtta tctcaatatt gaacgcttga gaactgaggg tgaacaatta
      241 ggtgatgaat taaatgatct aattaaaggt ttagaaacgg tcgaagattc aataggtaat
      301 aacgaaagtg attacgagaa aattatcgaa ctaaataata cgataacgaa cataaataat
      361 gaaatta
//
LOCUS       DAMZLE010000335          364 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMZLE010000335 DAMZLE010000000
VERSION     DAMZLE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.345"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            36..>364
                     /locus_tag="POB92_002867"
     CDS             36..>364
                     /locus_tag="POB92_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HDI6580278.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRD"
BASE COUNT          132 a           49 c           72 g          111 t
ORIGIN      
        1 ttatagtata attttaacaa aaaggagtct tctgtatgaa ctatttcaga tataaacaat
       61 ttaacaagga tgttatcact gtagccgttg gctactatct aagatatgca ttgagttatc
      121 gtgatatatc tgaaatatta aggggacgtg gtgtaaacgt tcatcattca acggtctacc
      181 gttgggttca agaatatgcc ccaattttat atcaaatttg gaagaaaaag cataaaaaag
      241 cttattacaa atggcgtatt gatgagacgt acatcaaaat aaaaggaaaa tggagctatt
      301 tatatcgtgc cattgatgca gagggacata cattagatat ttggttgcgt aagcaacgag
      361 ataa
//
LOCUS       DAMZLE010000336          358 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMZLE010000336 DAMZLE010000000
VERSION     DAMZLE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.347"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..156)
                     /locus_tag="POB92_002868"
     CDS             complement(<1..156)
                     /locus_tag="POB92_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6580279.1"
                     /translation="MTFEEKLSQMYNEIADEISGMIPVEWENIYTIAYVTDQGGEVIF
                     NYTKPGSD"
     gene            complement(167..>358)
                     /locus_tag="POB92_002869"
     CDS             complement(167..>358)
                     /locus_tag="POB92_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDI6580280.1"
                     /translation="FTRDGKLNVVFDYVDWMNSEFGPSGKENYYMYKKFGVLPETEYE
                     INKVKEIEQYIKEQDEAEL"
BASE COUNT          102 a           77 c           36 g          143 t
ORIGIN      
        1 atcgctacct ggtttagtat aattaaaaat gacctctcca ccttgatcag ttacataggc
       61 aattgtatat atattttccc attctactgg tatcattcca ctgatttcat ctgcaatttc
      121 attgtacatt tgacttagct tttcttcgaa agtcatgtta tcgcccctat agttcagctt
      181 catcttgctc tttaatatat tgctcgattt ctttaacttt attaatttca tattccgttt
      241 ctggtaaaac accaaacttt ttatacatat agtagttttc ctttcctgat ggaccaaatt
      301 ctgaattcat ccaatcaaca taatcaaata caacattcaa tttaccatct cttgtaaa
//
LOCUS       DAMZLE010000337          348 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMZLE010000337 DAMZLE010000000
VERSION     DAMZLE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.348"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          121 a           59 c           34 g          134 t
ORIGIN      
        1 gtatactgtt ataactgaaa tcttctatat atcatttcgt aatatcgcta gcttcatcta
       61 aaaaatttaa tgctatgatt tagccgaatt aaaaacatat atacttattt actatgaact
      121 aaaagtttat cctaaaaata aaaaaccatg atttcgaagt cttcataaac tgaagcttct
      181 actctcatgg caatttatac tatgatattc aattataatg cctagtctca tagtaaacgg
      241 ctactacatt cgcatgtact tcgttttact gtccacgcat ggtcgttgac ttatttatat
      301 tataatataa atattttatt tttcaatact taattttaaa aatcccct
//
LOCUS       DAMZLE010000338          343 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMZLE010000338 DAMZLE010000000
VERSION     DAMZLE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.349"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..272
                     /locus_tag="POB92_002870"
                     /pseudo
     CDS             1..272
                     /locus_tag="POB92_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          146 a           47 c           65 g           85 t
ORIGIN      
        1 gtggaggtag tcgagaagga ctaaacttca aagaaattgc taatttaaaa atcttcaccc
       61 caactatatt tgaagagcag caaaaaatag gcgaattcat cagcaaactt gaccgacaaa
      121 ttgaattaga agaacaaaaa cttgaattac ttcagcaaca gaaaaaaggc tatatgcaga
      181 aaatcttctc gcaagaattg cgattcaaag atgaggaagg taaagattat ccagattgga
      241 aatcaaaatc aattcaagaa atatttgaga ataagggtgg cactgctcta gaaacagaat
      301 ttaattttga cggtaattat aaagttataa gtataggaag tta
//
LOCUS       DAMZLE010000339          341 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMZLE010000339 DAMZLE010000000
VERSION     DAMZLE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.350"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>341)
                     /locus_tag="POB92_002871"
     CDS             complement(<1..>341)
                     /locus_tag="POB92_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6580281.1"
                     /translation="GKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDE
                     MKNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLL
                     SRKGKRKIFLA"
BASE COUNT          105 a           60 c           35 g          141 t
ORIGIN      
        1 tgtgcaagga aaattttcct tttacctttt cttgataata gatactgtcc aaaaaaatta
       61 taataatact cttttttcaa tctaataata catacatgtt gatttaaatt agcatgcgtt
      121 tcaactatcg aattaatggc tgttctacct attgatgctc ctgtaatatt taaaagaaca
      181 tcaccatagt acgttctact atttttcatc tcatcatcta tatctttact aatataaact
      241 aagtcattaa gatttaattt accatttcta atattttgac tccttaaaaa tggtatgcct
      301 ttgtttgtat agttttcact tccacctttg ggagtctttc c
//
LOCUS       DAMZLE010000340          335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMZLE010000340 DAMZLE010000000
VERSION     DAMZLE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.351"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(95..220)
                     /locus_tag="POB92_002872"
     CDS             complement(95..220)
                     /locus_tag="POB92_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDI6580282.1"
                     /translation="MTVYFYIWIKKEYLASEIFDLTESEKQEINHQMIDEIEEEK"
BASE COUNT          105 a           54 c           29 g          147 t
ORIGIN      
        1 tattgtgtct gtacccaaaa tgtacccagc catttttagc gtgttttcaa caaaaaaacg
       61 ctagcttttt gctaacgttt aagaaaattt atttttattt ttcttcttca atttcatcaa
      121 tcatttgatg attaatttct tgtttttcac tttctgttaa atcaaaaatt tcactcgcta
      181 aatactcttt tttaatccaa atataaaaat atactgtcaa atcaccttct tttagaaaat
      241 gccatttggc atgttttccg ttctatttta tttctttaga cagtctaaaa taaataatac
      301 tttgcttttt atttaacatt ttaaagttaa ttaaa
//
LOCUS       DAMZLE010000341          332 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMZLE010000341 DAMZLE010000000
VERSION     DAMZLE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.352"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(86..301)
                     /locus_tag="POB92_002873"
     CDS             complement(86..301)
                     /locus_tag="POB92_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580283.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT          102 a           50 c           39 g          141 t
ORIGIN      
        1 tgcttttcta tccaataaaa aagaagagaa gatgtaacac atcttctctt ctgcaatatt
       61 aattaggatt tatttctaag ttgagttatt ttaattgtaa atctgttttc tttaattctt
      121 ttataacttc tgcagtatca taacaatttg ttgcaattgt tgaatatctc tctgctaaac
      181 gatatgcatt aatgtaaagc tttaaacttt ctttagctat atcctctgca tcttcgaatt
      241 ttgatgggtt agacataacc actaattctg caaatttttc tggatcaata ttaatagaca
      301 tgtatttatt tacaactcct atttattttg at
//
LOCUS       DAMZLE010000342          331 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMZLE010000342 DAMZLE010000000
VERSION     DAMZLE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.353"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT           66 a           82 c           52 g          131 t
ORIGIN      
        1 tcgtcagatt caaacgtttt cacttcgcca agccattttt ctttgtgttt gcttttattt
       61 tgacgtttta gacataaaaa aagagacctt gcggtctcaa cttgcctggt aacgttctac
      121 tctagcggaa cgtaagtttg ctaccatcgt cgctaaagac ctttcttgac ttgtgacaat
      181 cgcttgcttc tttcctctcc ttcggctctc gcttactcat ttagctctac taaactcgtt
      241 gcgctctttt cttgtttcgt cagattcaaa cgttttcact tcgccaagcc atctttcttt
      301 gtgtttgctt tttactatgt aactttagac a
//
LOCUS       DAMZLE010000343          321 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMZLE010000343 DAMZLE010000000
VERSION     DAMZLE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.355"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..224
                     /locus_tag="POB92_002874"
     CDS             <1..224
                     /locus_tag="POB92_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6580284.1"
                     /translation="ESDADSDTDSDSDAGKHTPAKPMSTVKDQHKTAKALPETGSENN
                     NSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT          125 a           51 c           58 g           87 t
ORIGIN      
        1 cagaaagtga tgccgattca gatacagata gcgattcaga tgcaggtaaa catactccgg
       61 ctaaaccaat gagtacggtt aaagatcagc ataaaacagc taaagcatta ccagaaacag
      121 gtagtgaaaa taataattca aataatggca cattattcgg tggattattc gcggcattag
      181 gatcattatt gttattcggt cgtcgtaaaa aacaaaataa ataatatgat taacttaacc
      241 aggtccatgt ggcctggttt tttctgttta gaaattccca gatataaaac aaaaaacctt
      301 taaggaatag atacaattta a
//
LOCUS       DAMZLE010000344          318 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMZLE010000344 DAMZLE010000000
VERSION     DAMZLE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.356"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..167
                     /locus_tag="POB92_002875"
     CDS             <1..167
                     /locus_tag="POB92_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6580285.1"
                     /translation="VKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLL
                     LFGRRKKQNK"
BASE COUNT          127 a           48 c           46 g           97 t
ORIGIN      
        1 ctgttaaacc aatgagtact actaaagacc atcacaataa agcaaaagca ttaccagaaa
       61 caggtaatga aaatagcggc tcaaataacg caacgttatt tggcggatta ttcgcagcat
      121 taggatcatt attgttattc ggtcgtcgta aaaaacaaaa taaataatac agcttatacc
      181 aggtccgtga ggcctggttt tttatttcaa aatttatgaa atgtatctta tgtttgagat
      241 taaatagtgc aattttttaa aaaatataac ataaatcaac ataaatttaa ttcatcttta
      301 cattaaaagt acaaacga
//
LOCUS       DAMZLE010000345          287 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMZLE010000345 DAMZLE010000000
VERSION     DAMZLE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.359"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>287)
                     /locus_tag="POB92_002876"
     CDS             complement(<1..>287)
                     /locus_tag="POB92_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580286.1"
                     /translation="TVIIPNLSLLPSYLSPHFNHQHYSELESFEEQIFLEDTVFDKVK
                     NFLSSSYFLILFVGYLSQRNYIDIMIFIIIFNILALILVSLFIRFSKQKIT"
BASE COUNT          128 a           27 c           38 g           94 t
ORIGIN      
        1 ttgttatttt ttgtttagaa aacctaataa acaacgaaac aagtattaag gctaaaatgt
       61 tgaaaataat tataaaaatc attatgtcaa tataattacg ttgagataag taacctacaa
      121 atagaattaa aaaatatgat gacgataaaa aattcttaac tttatcaaaa acagtatctt
      181 caagaaaaat ttgttcttca aaagactcta attcactgta atgttgatga ttgaaatgtg
      241 gagataaata cgaaggtaat aatgataaat taggaattat tactgta
//
LOCUS       DAMZLE010000346          284 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMZLE010000346 DAMZLE010000000
VERSION     DAMZLE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.360"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          105 a           51 c           23 g          105 t
ORIGIN      
        1 cactttaata gtcatcttca ttttactaat ggtgagaaag taatatttta ttcaaagaat
       61 atgacttcat attaaatcaa acctcacatt cttattagat tgcaaattca atagtcctac
      121 cagaattgaa atcaaaacat tcaaactatt ttatcgtttt aacttcctcg accattttca
      181 cctccttata atctatataa attatgttta ccacacgatt ctttacactc gaaaaattaa
      241 ttttgttaag aaacatttag ccatgtaact ttgtaataaa aaca
//
LOCUS       DAMZLE010000347          275 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMZLE010000347 DAMZLE010000000
VERSION     DAMZLE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.denovo.361"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>275
                     /locus_tag="POB92_002877"
     CDS             <1..>275
                     /locus_tag="POB92_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580287.1"
                     /translation="EFIDQGTAILNILDLNDLETYFNDYLETINHTTEFAKKIANKAM
                     KEIRKNIELEQIKQQVSYMKNADQVSSLESFKDLKHTILKQHRDLSL"
BASE COUNT          121 a           36 c           39 g           79 t
ORIGIN      
        1 tgagtttatt gatcaaggta cagccatttt gaatatatta gatttaaatg acctagaaac
       61 atattttaat gattacttag aaacaatcaa ccatacaaca gaattcgcta aaaaaatagc
      121 taacaaagct atgaaagaaa ttcgaaaaaa tattgagtta gagcaaatta aacagcaagt
      181 aagttatatg aaaaatgcag atcaagtctc ttctttagaa agctttaagg atttaaagca
      241 tacgatatta aaacaacacc gagatttatc gttaa
//
LOCUS       DAMZLE010000348        11487 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.082, whole genome shotgun sequence.
ACCESSION   DAMZLE010000348 DAMZLE010000000
VERSION     DAMZLE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.082"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(87..1592)
                     /gene="qac"
                     /locus_tag="POB92_002878"
     CDS             complement(87..1592)
                     /gene="qac"
                     /locus_tag="POB92_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound
                     efflux MFS transporter"
                     /protein_id="HDI6580288.1"
                     /translation="MKKRWTALVVLAVSLFVVTMDMTILIMALPELVRELEPSGTQQL
                     WIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFAESAEFVIAIRF
                     LLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPIIGGALLEQFSW
                     HSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIGLVWSIKEFS
                     KEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTIAAFMTMFA
                     MASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPKIVLPSGI
                     GIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKAGNAAAV
                     EESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGAVEVAK
                     ATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
     gene            1808..2374
                     /gene="qacR"
                     /locus_tag="POB92_002879"
     CDS             1808..2374
                     /gene="qacR"
                     /locus_tag="POB92_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator
                     QacR"
                     /protein_id="HDI6580289.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFK
                     TKENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEF
                     YTEYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIV
                     TFTHEQNINERIKLMNKFSQIFLNGLSK"
     gene            complement(2556..3089)
                     /locus_tag="POB92_002880"
     CDS             complement(2556..3089)
                     /locus_tag="POB92_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6580290.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNE
                     SKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEV
                     NITRQTIYRIKNDNDLI"
     gene            complement(3458..4303)
                     /gene="blaZ"
                     /locus_tag="POB92_002881"
     CDS             complement(3458..4303)
                     /gene="blaZ"
                     /locus_tag="POB92_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDI6580291.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKTGDTLIKDGVPK
                     DYKVADKSGQAATYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            4410..6167
                     /gene="blaR1"
                     /locus_tag="POB92_002882"
     CDS             4410..6167
                     /gene="blaR1"
                     /locus_tag="POB92_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDI6580292.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSLFKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAETIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNVPFFN
                     KNILSHSFNGKKSLLKRRLINIKEANLKKQSKLILIFICIFTFLLMVIQSQFLMGQSI
                     TDYNYKKPLHNDYQILDKSKIFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPNSTYK
                     IYLAMFGLDRHIINDENSRMSWNHKHYPFDAWNKEQDLNTAMQNSVNWYFERISDQIP
                     KNYTATQLKQLNYGNKNLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGK
                     NAELISEKILKEMGVLNGQ"
     gene            6157..6537
                     /gene="blaI"
                     /locus_tag="POB92_002883"
     CDS             6157..6537
                     /gene="blaI"
                     /locus_tag="POB92_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDI6580293.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            6801..7394
                     /locus_tag="POB92_002884"
     CDS             6801..7394
                     /locus_tag="POB92_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6580294.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            7366..8809
                     /locus_tag="POB92_002885"
                     /pseudo
     CDS             7366..8809
                     /locus_tag="POB92_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            8802..9617
                     /locus_tag="POB92_002886"
     CDS             8802..9617
                     /locus_tag="POB92_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6580295.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(9845..>11487)
                     /locus_tag="POB92_002887"
     CDS             complement(9845..>11487)
                     /locus_tag="POB92_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HDI6580296.1"
                     /translation="NILGIIGAFYFKYLVDTIIPSQIIQNLHILSIAVLFIYLINALV
                     SLIRYQASLNLSLKIDMNFMKDYYYHVLNLPIKFYETRKSGEILSRFSDLSHIREALS
                     SVTITLLVDTLMVLVGGTALFFQSALLFYITLILIPIYLIIGLSFRKILSKYNRLVME
                     QDATLSSYLVESFSGYSVIKSFGAENEIFNKGVNHFKKLIRRLYKLSFFTNMQLTINT
                     FMKMTTTLIILWIGSFLIIKNQLTLGELLTFNALVIYYIDPIERLINLQPQIQSAIVA
                     TQRYLDITDIKTENEERNNNNTLIDSTFTFTNKLEIRNLNFQYNFKEEALKNINISIL
                     KNQKVAIIGESGSGKSTIAKLIDNFHINYEGSITLDNTSIKEIPKNKLRNLISFVTQQ
                     NFIFGATIRENLTIGLNRKVSDSDIYKACKIVCAYDFIINLPQKLDTQLHNGGSNLSG
                     GQLQRIALARAILKDSDILLLDEATSSLDASTEKNILENIDLHLSSKTIILITHKLTN
                     VKNADNIYLLKYGQIIEEGTHKSLINKKGEYYNLWKNQ"
BASE COUNT         4260 a         1629 c         1656 g         3942 t
ORIGIN      
        1 catttaaaat taatggttct actttttaac tattactaat tttaaaaata aactaatcta
       61 caatatctaa aaatatatgt ttagtactat ttttgtttag ttatatcaag tgatttggga
      121 attaacaaat agacaactat tgaaatgata atcatgataa tcccacctac taaagcagtt
      181 gctacaaaag catcattaaa tgatgttaca gcctcgtttg caagctgttt tatccccgta
      241 gctttagcta cttcgacagc gcccactaca gattcttcag ctacatgagc taaatctcca
      301 actatacctt ttgatgaaaa agatgaaata tctaaaaata cacgataaag catagaagat
      361 aggctaccaa gtactgctac accaaaaaca tttccaaggt catacataga ctcttcaaca
      421 gcagctgcat tacctgcttt tgatgtaggt gtttctaaca ttattagagc agatgcaact
      481 gctagtgaag ccataccagc tccaactaaa attaatgcta aagccattgt agaatatgat
      541 aatggatgac caaagaaata cataataaac atgccaatgg ctgcaattcc aattccggaa
      601 ggtaacacta ttttcggtcc aaatcgcgcc gctaatccgg gtgcaattgg tgcaaacacc
      661 atatctccta ttgccatagg taataggtat aagccagctt taaaaggaga aagttcttcc
      721 acaacctgta accattgtga agctaataac aaaacagatg ccattgcaaa cattgtcata
      781 aatgcagcaa ttgtaccagc tgaaaatgat ctctttttaa aaagtcttac gtctaacatt
      841 ggatcagaac ttgataaatt acgtttaaca aatatcacta tcatggtaat tgctaatact
      901 attacaaccc atggaataat atctgctagt ccttcttttg aaaattcttt gatactccat
      961 accagtccaa tcatgcctgc aattgataaa attgtagaag gaatatccca cgagtgagac
     1021 ttttcttttg ataacttaga ctctggtaat aaaaataaac ctgctacaac tgctattatc
     1081 gcaaacggta cattaattaa aaatgccgag tgccatgaaa attgctcaag taaagctcct
     1141 ccgataattg gtccaaaaac agcacctatc gatgaagcga ttgaccatac agctaatgca
     1201 gtggcccttt ctttagggtt ttcaaaaatt actcttatca ttgaaagagt agttggcatt
     1261 attaaagcac ctgcaatacc aagtaaaaat cgaatagcta ttacgaactc tgcactttct
     1321 gcgaaaaata tagctaatga aacgaggcca aataaagcaa atccagttaa taatgctttt
     1381 tttcttcccc atttatcagc aaaggcactc aatggaatta taaagccagc taaaacaaga
     1441 gagtatatat caactatcca taactgttgg gtaccagaag gctctaactc tcttactaat
     1501 tccggtaaag ccataattaa tattgtcata tccattgtaa caacaaacaa actaacagct
     1561 aatactacta gtgcagtcca tctttttttg atgtcatcat atcagtagtt tttgtaaaaa
     1621 atgaaatcat ttaagttccc tccaatcctt atagaccgtg cgatcggtct ataaggatta
     1681 taatctattt ttttacatat tacaacaaaa aatcttgcga aatttaaatg gctgtattgt
     1741 gttatacaat tattctttga attttttgtg ctatcattga tagtacttaa tactcattaa
     1801 aggcgtgatg aacttgaaag ataaaatact aggtgtcgca aaggaattat ttataaaaaa
     1861 tggatataat gccactacta ctggagaaat tgttaaatta tcagaaagta gtaaagggaa
     1921 tctttattat cactttaaaa caaaagaaaa tctattttta gaaattttaa atatagaaga
     1981 atctaaatgg caagaacagt ggaaaaagga acaaatcaaa tgtaaaacta atagagaaaa
     2041 attttattta tataatgaac tatctttaac aaccgaatac tattatccac ttcaaaacgc
     2101 aataattgaa ttttacactg aatattataa aactaatagc attaatgaaa aaatgaataa
     2161 attagaaaac aaatatatag atgcttatca tgtaattttt aaggagggta atttaaatgg
     2221 cgaatggtgt attaatgatg ttaatgctgt tagtaaaata gcagcaaacg ctgttaatgg
     2281 aattgttacc tttacacatg aacaaaatat taatgaaaga attaaactta tgaacaagtt
     2341 ctctcaaatt tttttaaatg gacttagtaa ataattatat atatattctt gaaataccaa
     2401 taaatattat ttctaaatta cgttcgttaa tgagaaaaag agagagcata tttacgctct
     2461 cttttcttgt cttattatta aaaactcata atagattata atcatctgtt ttgagacgct
     2521 gtgagaagct ctcagttgca ttgaaacaaa gtttattaaa tcaaatcatt atcgttttta
     2581 attctgtaaa tcgtctgcct tgttatattt acttctttcg caattttact aattgattgg
     2641 ccttcttcta acatttctac aacgcgatga taaataatac gtttctgtgg atctttagca
     2701 tttggtgaat aaagtaaggg gcgcccttta tatacgcctt tttccttcgc aacttgaatt
     2761 ccttgcgctt gtcgacgttt actttcattt ctctcttgtt ctgaaaccat tgctaatatt
     2821 tgaataatta aatctttcat aaacttatcc agtaatggat tgccaaccac ttcatcataa
     2881 ttacgaccta aacaatcaat ggattctact acaaaccgat ctcccatcct caaaaagtta
     2941 agtgcttctt gaagtactgg tctattttca atagatttcc cagactgttt ttctgtaaat
     3001 attttttcag cacaaaacgt ctttaaattt tcaagttgtc tttctaaatt ttgatcgttc
     3061 gatgatactc tcgcatagcc aacaatcatt gatttccatc tcataattaa tatacaccct
     3121 aatcatacgt ttcagttact ataaattcaa caattatgtc actttgcttg atatatgaga
     3181 attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt
     3241 tcgattgatg aacacctata attaaactat tcatctatta tttatgattt tttgtatata
     3301 caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata atagagggaa
     3361 aatcagtttt tgatatcaaa attatacatg tcaacgataa tacaaaatat aatacaaaat
     3421 ataagatgtt atcagtattt attatgcatt tagaatatta aaattccttc attacactct
     3481 tggcggtttc acttatcaac ttatcatttg gcttatcact tttattgtct ttattcgtaa
     3541 aaatgactaa aacaataggt tcagattggc ccttaggata aacaaaagca acatcatttc
     3601 tagaagcata tgttgctgct tgaccacttt tatcagcaac cttatagtct tttggaacac
     3661 cgtctttaat taaagtatct ccggttttat tatttaacat taaatcaagt aagaattttt
     3721 tgttttcttt gcttaatttt ccatttgcga taagtttatt taaagtctta ccgaaagcag
     3781 caggtgttga agtatctttt ttgctctttg gtgaatagta atttaattct atctcatatc
     3841 taactggatt tgttacttta tctcctagtt cttttagacg ttgtttaact tttttgattc
     3901 caccgatttc ttttataatt ttattgtttg ctgtattatc actatatgtc attgaagcct
     3961 caataagtgc ttttaaagtg atatcttttc ctacatattt ttctaaaata ggagaataag
     4021 caactatatc atctttgtta atatgtactt ttttatttaa cttattataa ggtacttgtt
     4081 ctaacaaaat agcactattt atcgcttttg aagttgaagc ataggcaaat ctcttatctg
     4141 aattaaattt tacttcctta ccacttttag tatctaaagc ataaacacca atatgagcat
     4201 tatatttttt ttctaaatca tttaactctt tggcatgtga actgtttgaa ttacatgcac
     4261 ttaaaactaa agcaattaca attaaaaata ttaacttttt caaaataaac cctccgatat
     4321 tacagttgta atttttataa atcaataata ttacaattgt aatatcggtg tcaatagttt
     4381 attataaata tttgaaagaa ggtgttgaaa tggctaagtt attaataatg agcatagtaa
     4441 gcttttgttt tatatttcta ttgttattat tttttaggta catattaaaa cgctatttta
     4501 attatatgtt aaattataaa gtttggtatc taactcttct tgcaggatta attcctttca
     4561 ttcctattaa attctctctt tttaaattta ataatgtgaa taatcaagcg cccacagttg
     4621 aaagtaagtc acacgacttg aaccataaca taaataccac caaacctatt caagagttcg
     4681 caacagatat ccataagttt aattgggatt caattgataa tatctgcaca gttatttgga
     4741 tagttttagt tattatttta agttttaaat ttttgaaagc cttattatat cttaaatatt
     4801 taaagaaaca gtcactttat ctaaacgaaa atgaaaaaaa taaaatagat acgatacttt
     4861 tcaaccatca atataaaaaa aatattgtga ttcgaaaagc agagactatt caatctccaa
     4921 taactttttg gtatgggaaa tatattattt tgattcctag ttcatatttt aaaagtgtaa
     4981 ttgacaaaag actaaaatat ataatattgc atgaatatgc tcatgctaaa aatagagata
     5041 ctttacattt aattatcttt aatatcttct ctattattat gagttacaat ccattagtac
     5101 atattgtaaa aagaaagata attcatgaca atgaagtaga agccgataga tttgtcctta
     5161 ataatattaa caaaaatgaa tttaaaactt atgcggaatc tattatggac tccgtattaa
     5221 atgttccatt ttttaataaa aatatattaa gccattcatt taatggtaaa aagtcattac
     5281 tcaaaagaag attaattaat ataaaagaag ccaatctgaa aaaacagtcc aaattaattc
     5341 taatttttat ttgtattttt acttttttgc tcatggtaat tcaaagccag tttttgatgg
     5401 ggcaatccat aactgattat aattataaaa agcccttaca caacgattac caaattttag
     5461 ataaaagtaa aatttttggt tcaaatagtg gatctttcgt catgtacagc atgaagaaag
     5521 acaaatacta tatttataac gaaaaagaaa gtagaaaaag gtattctcct aattcaactt
     5581 ataaaattta tttagctatg tttggacttg accgacatat tataaatgat gaaaattcac
     5641 gtatgtcatg gaatcataag cattaccctt ttgatgcttg gaataaggaa caagatttaa
     5701 atacagcaat gcaaaattca gttaattggt acttcgaacg tattagcgat caaataccaa
     5761 agaactatac tgcgactcaa ctcaagcaat taaattatgg taataaaaat ttgggaagtt
     5821 ataaaagcta ttggatggaa gatagtttga aaatatctaa tcttgaacaa gtaatagttt
     5881 ttaaaaatat gatggaacaa aataaccatt ttagtaaaaa agcaaagaat caattatctt
     5941 cttcattatt gattaagaaa aatgaaaagt atgaactgta tgggaaaaca ggtacaggta
     6001 tagtaaacgg gaagtataat aatgggtggt ttgtaggtta cgtaattaca aatcatgata
     6061 agtattattt tgctacacat ttatcagatg gaaagccatc tgggaaaaat gctgaattaa
     6121 tcagtgaaaa aatattaaaa gaaatgggtg ttttaaatgg ccaataagca agttgaaata
     6181 tctatggctg aatgggatgt tatgaatata atatggggta aaaaatcagt atcagctaat
     6241 gaaattgtag ttgaaattca aaaatataaa gaagttagcg ataaaacgat tagaacatta
     6301 atcacaagac tatataaaaa agagattata aaacgataca aatcagagaa tatttatttt
     6361 tactcatcaa atattaaaga agacgatatt aaaatgaaaa ctgctaaaac ctttcttaat
     6421 aaactgtatg gaggggacat gaaaagttta gtgctgaatt ttgcgaaaaa tgaagaatta
     6481 aataacaaag aaattgaaga attgcgagac attttaaatg atattagtaa aaagtaatta
     6541 gagatatgtt tcaaacataa tacatatgta aactatgctt gactcttatt aaattagttt
     6601 tgttaataac atttaaaata tattattaaa gatgagagtg tacagaaaat aaccattttc
     6661 tgtacactct tcttttatgt ctaaataagt aagaattaca taggattatg tgttcaaaaa
     6721 cccaacaaaa aatctatgtt cataaaagac cttgtaatta agttatggta cactgaagat
     6781 gtatatagga ggtttgtatt ttgaaaatag gttatgcaag agtttcaact ggattacaaa
     6841 atttaaattt acaggaggat cgattaaata catatggttg tgaaaagata tttaatgacc
     6901 atatgagtgg ttcaaaaagt aaaagacctg gtttagataa agcaattgaa tttgcgaggt
     6961 caggagatac gattgttgtt tggagattag atagattggg acgtaatatg gaggatttaa
     7021 tcacattagt taatgagctt aacgaacgag gtgtaagttt ccatagttta gaagaaaata
     7081 taacaatgga taaatcaagt tcaactggac aattattatt tcatttattc gcagcatttg
     7141 cagaatttga acgtaattta attttagagc gttcttcagc tggccgaatt gcagcacgtg
     7201 ctagaggccg atatggaggc agaccagaaa aattaaatca aaaagattta aacttactta
     7261 agacccttta tgataatggc acgccaatta agacaattgc agaacagtgg catgtttcac
     7321 gtacaacaat ttatcgttac ctcaataaat tggaggacaa ggaagatgaa aaacaaggag
     7381 aagtatctaa ctaatttttc tgaagccaaa cgtaaaaaag ctactcaaaa gtacaatatt
     7441 ataaaacctt ttatcctagg cgaacaatct ttatcaagta tttctaaaag taaagggatt
     7501 gcgttaagca ccctttatag gtggaataag tcatataaag aacaaggact taaaggacta
     7561 atctatgcga caagagctga taaaggaaca cgtaaaatag aaccaaagat aattgatgaa
     7621 attgaacgcc ttgcgcttat gaataaaaga aatagtatag ccacaattca taggaaaatc
     7681 actaattact gtaaagaaaa taattttgat ataccaagtt ataaacaaat ttatagtgtt
     7741 attaaagcga tgcctaagtc tgttattgat ttctctcatc agggtgaaaa atactatcaa
     7801 aataagtacg atttaataca aattagagaa tcctcgagac ccaatgaaat atggcaagca
     7861 gaccatactt tgctagatat ctttatatta gaccaaaaag gtaatatcaa tagaccatgg
     7921 ctaactatta ttatggatga ctatagtcgt gcaattgcag gctatttctt aagttttgat
     7981 gccccccaat gctcaaaaca cagcattaac actacaccaa gcaatatgga ataaaaatga
     8041 taccaattgg ccagtgtgtg gtatacccga aaaattctac acagatcatg gaagtgactt
     8101 tacttcacat catatggaac aagtcgctat tgatttgaaa attaacttga tgttttcgaa
     8161 agttggtgtt cctcgtggtc gtggaaaaat agaacgattc tttcagacag ttaatcaaac
     8221 tttcttagaa caactgcctg gatatataaa caataatgat actcctagta atctaataaa
     8281 ttttcacaat tttgaagaaa aattacgtta ctttttaatt gaagattata atcaaaaaga
     8341 acatagtact attcatacca cgcctatcag tcattggaat agtaatcact ttttccctaa
     8401 tatgccaagt agcttagaac aacttgattt attattgttg gaaataccta aatctagaaa
     8461 agttcattca gatggcattc attttcaagg ttttcgatac agtaatacta atctagaagc
     8521 ttatgtaggt gaatatgtac tgattcgtta taacccaaat gatatggctg aaatacgtgt
     8581 gttttataga gatgaatttc tttgtactgc tatatgtcca gaaatagctg attactccat
     8641 agatataaaa gacatacagc acgctcgtag tcagagaaga aaacacttaa aacaaaatat
     8701 tgctagtcca agtaccacgg aattaattaa ggaagaaaaa aattgtggtt attcacctca
     8761 agaaacgact aaaaacgtca aaaaattaaa gagatatcgt aatgactaaa aatcaaaatt
     8821 tcattgaaac gaaagagtat aaacgctttg ctgaattttg tgatgcttgt attaaatatc
     8881 agtatattgg tatatgttat ggtcaacctg gtgtcggaaa aactttatcg tctaggtatt
     8941 acacaaattg ggatactatt gaaaaacaag ttaaccatag aggttgggaa gatttagcta
     9001 gtaaaacgac cgatgacata ctatcggtgg ataaaatatt ttataatgca ccagcagaaa
     9061 aacaaactaa attaaggaat gatttatata gtattactgc aatcattgat ttgggtcaaa
     9121 aattacatgt tgtaaataag tatggacatg aacatagtaa acattatagt gatatgttta
     9181 aatatattga tttaattata gtagatgaga ttgatcgcct taaagtgcaa catttagagc
     9241 aattaagagc tatttatgat gaacataatt tagcaatgat atttattggt atgccgggta
     9301 tagagaaaaa actatctcgt tatcctcaac tatattcgcg tataggtttt gcgcatgaat
     9361 ttgataatct aagtaaagat gagacgcatc atatattaga atataagtgt caagacttag
     9421 gatttgattt aaaactcgaa gactttactg attatgaggc aataacgacg attattaaaa
     9481 ttacaaaagg gaattttagg ctgattcatc gtttatttgc gcagatagac agaattatgg
     9541 atataaatgg tttagataaa ataagtacag aagttgtaga aacagctaga gatagtttag
     9601 ttataggtat tcgataatag aaaaagcaac gcattttaat atagtgcgtt gcttttctca
     9661 tttattaggt aaagtcattc tcatatatcg agtaaagtga caacaattat atatgtatga
     9721 ttagggtgta ctcaaagtat acatatctat aattaaaact tactaaaaat aaatatagat
     9781 tttttctttt atatttatta gaggtgaatc tttcattaag aatggttaaa gtaaatcatt
     9841 aaccttattg atttttccat agattatagt attcaccttt tttattgatt aatgatttat
     9901 gagttccctc ttcaattatt tgaccatact ttaataaata aatgttgtca gcatttttaa
     9961 cattagtcaa tttatgtgtg attaaaatga ttgttttgct tgataaatga agatctatat
    10021 tctctaatat atttttttca gtagatgcat ctagagaact ggtagcttca tctaataata
    10081 atatatcgga atcttttaat attgctctag ctagtgctat tctttgtaat tgtccaccag
    10141 ataaattaga acctccatta tgtaattgag tatctaattt ttgtggaagg ttaattataa
    10201 aatcatatgc acaaactatt ttacaagctt tatatatatc agaatcagag acttttctat
    10261 ttaatccaat agtcaaattt tctctaattg tagctccaaa aataaaattc tgttgtgtaa
    10321 caaaactaat caaatttctc agtttatttt tgggaatttc ttttattgaa gtattatcaa
    10381 gcgttatact accttcataa tttatatgaa aattatcaat taattttgct atagtagact
    10441 ttcctgaacc actttcacct ataatagcaa ctttttgatt tttgagtatt gatatattaa
    10501 tgtttttcaa agcttcttct ttaaaattat attgaaagtt aagattccta atttccagtt
    10561 tatttgtaaa agtgaaagta gaatctatta atgtattatt attatttctt tcttcatttt
    10621 cagttttaat atctgttata tctaaatatc tttgcgtagc gacaattgct gattgtattt
    10681 gtggctgtag gtttattaat ctctcaattg gatctatata atatattact aaagcattga
    10741 acgttaaaag ttctcctaaa gtcaactgat tttttattat aagaaatgat ccaatccaaa
    10801 ggataattaa agtcgttgtc attttcataa aagtgttaat agttagttgc atattagtaa
    10861 aaaaactaag tttatacaat cttctgatta attttttaaa gtgattaact cctttattaa
    10921 atatttcatt ttctgctccg aaacttttta ttactgaata acctgaaaaa ctttcaacaa
    10981 gatatgaact taaagtagcg tcttgttcca tcactagtct attatatttg cttaatattt
    11041 ttctgaatga taatcctata attaaatata ttggaatcag aattaatgtt atataaaata
    11101 acagcgcact ttgaaagaat aaagcagttc caccaactaa aaccattaat gtgtctacta
    11161 ataatgttat agtaactgaa gaaagtgctt ctcttatatg agataaatca gaaaaccttg
    11221 ataaaatttc tccacttttc cttgtttcgt aaaattttat tggtaaattt aaaacgtgat
    11281 aataataatc tttcataaaa ttcatatcaa tttttagact taagtttaaa gaagcttgat
    11341 atctgattaa agaaaccaat gcattaatta agtatataaa tagtacagct atagataata
    11401 tatgtaaatt ttgtataatt tgagaaggta ttatagtatc cacaagatat ttaaagtaaa
    11461 atgcacctat aatacccaat atattta
//
LOCUS       DAMZLE010000349         5708 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.142, whole genome shotgun sequence.
ACCESSION   DAMZLE010000349 DAMZLE010000000
VERSION     DAMZLE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.142"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            291..3364
                     /gene="mupA"
                     /locus_tag="POB92_002888"
                     /pseudo
     CDS             291..3364
                     /gene="mupA"
                     /locus_tag="POB92_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
     gene            3434..4321
                     /locus_tag="POB92_002889"
     CDS             3434..4321
                     /locus_tag="POB92_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6580297.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLG
                     KIVANMHKNINSLNLFGFSHNFNGGTIEFTNKWTSSWGDLFIKSRMDKLCSEIYKKRL
                     FSISDLLLYEDIRKIMLKSLYYHQSSPSLLHGDLWKGNILFQKNGDPILCDPVCLYGD
                     REFDIGYTLALEKFPLDFYQEYNNIYPLCVGYEKRIEFYKLYVFMIHLFAIGTDFYDK
                     VKDTMYNILNNYGVDFIKG"
     gene            4666..4959
                     /locus_tag="POB92_002890"
     CDS             4666..4959
                     /locus_tag="POB92_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6580298.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            5034..>5708
                     /locus_tag="POB92_002891"
     CDS             5034..>5708
                     /locus_tag="POB92_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6580299.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIH"
BASE COUNT         2183 a          695 c          883 g         1947 t
ORIGIN      
        1 tgtatagatg tgcatataat tttaactttg caacagaacc aaatttataa tagtatagtt
       61 tcaacatggg atgtttataa aaataacgaa tatagcaaag acctttcaag agaattaaaa
      121 tttaaattat atactactaa tgatataatt tgcagtagtt tgtattataa ctttacttta
      181 tccaatatat ctttctatgg agacatgaaa ggaaatgaat ttcatgaaaa ttcaccaaga
      241 ctaatagtaa aaataacgaa ataagtgata ctctaggagg ctgaaaagtt ttgacaaaga
      301 aatatttaaa cacccagaat gaaatatcag cattttggaa tactcaaaag atatttaaaa
      361 aatcaattga caatagaaaa ggacaggaaa gttttgtttt ttatgacggc cccccaactg
      421 caaatggcct tcctcatgct ggccatgttc ttggaagagt aatcaaggat ttagttgcaa
      481 gattaaaaac tatgcaaggt ttttatgtag aaagaaaagc aggatgggat acccatggct
      541 taccagttga attagaggtt gaaaaaaaat tggaattaaa ggaaaacaag acattgaaaa
      601 gtatggaata gaaaatttta taaatgaatg taaaaaaagt gtatttaatt atgaaaaaga
      661 atggcgggat ttttctaaag atttaggata ctgggttgac atggactccc cctatataac
      721 tcttgagaat aattatattg aaagtgtatg gaatatatta tctacattcc ataaaaaagg
      781 actattatat aagggacata aggtgactcc ttattgtaca catgatcaaa ccgctttaag
      841 ttctcatgaa gtagcgcaag gctataaaaa cgttaaagat ttatcagctg ttgttaaatt
      901 tcaacttaca aatagtaaag atacttattt cttaagttgg actaccactc cctggacttt
      961 gcctgcaaat gtagcattag ctataaataa agatcttaat tattcaaaaa ttcgggtaga
     1021 aaatgagtat tatatcttag ctacagatct aattaattct ataataactg aaaaatacga
     1081 aattattgat accttttcag gaagtaattt aattaattta aaatacattc ctccttttga
     1141 aagcgacggt ttagttaatg catattacgt tgttgatgga gaatttgtta ctaactcaga
     1201 aggaactggt attgttcata tagcaccagc tcatggggaa gatgactacc aattggtttt
     1261 agagcgtgat ttggatttct taaatgttat aacaagagaa ggagtatata atgataggtt
     1321 ccctgaatta gttggtaata aagctaaaaa tagtgatata gaaatcataa aattattatc
     1381 caaaaaacaa cttttatata aaaaacaaaa atatgagcat aattatcctc attgttggag
     1441 atgtggtaat cctttgatat attatgcgat ggaaggttgg tttattaaaa caactaattt
     1501 taagaatgaa attattaaca ataataataa tatagagtgg tttccttctc atattaagga
     1561 agggagaatg ggaaatttct tagaaaatat ggttgattgg aacattggta gaaatagata
     1621 ttggggaaca ccattaaatg tatggatttg caatgattgt aatcacgaat acgcaccaag
     1681 tagtattaag gatttacaaa ataattccat caataaaatt gatgaagata ttgagttgca
     1741 tagaccttat gttgataata tcactcttag ttgccctaag tgtaatggga aaatgtctcg
     1801 agtagaagaa gtaatcgatg tttggtttga tagcggctct atgccgtttg ctcagcatca
     1861 ttatcctttt gataaccaga aaatttttaa tcaacacttt ccagctgatt ttattgcaga
     1921 aggagttgat caaacgagag gctggtttta cagtttacta gtaatttcta ctattctaaa
     1981 aggaaaatct tcttataaac gtgctttatc tttaggacat attctagaca gtaatggtaa
     2041 aaaaatgtct aaaagtaaag gaaacgttat taatccaact gaattaatta ataagtacgg
     2101 agccgattct ttaagatggg ccttaatttc ggatagtgct ccatggaata acaaaagatt
     2161 ctcagaaaat atagtagctc agaccaaatc gaaatttata gatacgcttg ataatattta
     2221 taaattttat aatatgtata ataaaataga tcactataat cctaataatg aaattacaaa
     2281 aagtagaaat acattagata attgggctct ttctcgctta aacaccttaa taaaagaaag
     2341 taatatttat gtaaataatt acgatttcac ttccgcagcc agattaatta acgaatatac
     2401 caatacaata agtaattggt atattcggag atcgagagga cgattttggg aacaaggaat
     2461 ttctaacgat aaaaaagatg cgtacaatac gctttatgaa attttaacaa ctttatcaag
     2521 actagtggct ccatttgttc catttatatc tgaaaaaatc cattataatt tgactggaaa
     2581 aagtgtgcat ttacaagatt atccacaata taaagaaagt tttattaatc aagcattgga
     2641 agatgaaatg cataccgtta taaaaattgt agaattatct agacaggctc gcaaaaatgc
     2701 agatttaaaa attaagcaac ctttatcgaa aatggtgatt aaacctaata gtcaattaaa
     2761 cttaagtttt ttacctaatt actattcaat aataaaagac gaattaaata taaaaaacat
     2821 tgaattaact gataatatta atgactatat tacctatgag cttaaattga atttttcttc
     2881 tgtgggacca aaactaggga acaaaacgaa aaatattcaa acattgatag actccctatc
     2941 agagtatgat aaaaaaagtt taattgagtc taataacttc aaaagtttat cttctgatgc
     3001 tgagttaact aaggatgatt ttataattaa aaccttacct aaggatagtt atcaactcag
     3061 tgaagataat gactgcgtta tattattaga taaaaattta tctcctgaat taattcgcga
     3121 aggacatgct agagagctca ttagattaat tcaacaatta agaaaaaaga aaaatttacc
     3181 aataaatcaa cgtattgata tttatatcgg tgtaactggg gaattattag aatcaataaa
     3241 aaccaataaa aatatgttta aagaaaattt gctgattaaa aatatacact taaatgttat
     3301 agatgaatat gaaaatacta ttcattttaa taataaagaa ataaaaattt ccttattata
     3361 ttaaacaaac atggccactc tattttagta gagtggccat gagttatcaa agccaagcaa
     3421 aaggtgataa actatgtata attttgatta ttctaaatta ccaattaaaa atattcaaaa
     3481 aatatttcct attgcagggg gatatgtgaa tctatctttt tcagttgatg cttccaacaa
     3541 aaaatatttt ttaaagctac aacctaatac taaatcaaat ttttttgatt atgagttatc
     3601 cagtttaaaa gaattaactg ataaaaatat tcctgttcct caaattatta ataaaggaga
     3661 attagacaat aattcttttt tactgttaga gttcattgaa aatggccatg cttatcctga
     3721 aagctataga aaactaggta aaatagttgc aaacatgcat aaaaatataa attcactaaa
     3781 tctatttggc tttagtcata atttcaatgg aggaacgatt gaattcacta ataaatggac
     3841 ttcttcttgg ggggatttat ttattaaaag taggatggat aagctatgtt ctgaaatata
     3901 caagaaacgt ttattttcaa tttcggatct tttattatat gaagatatac gaaaaattat
     3961 gttgaagagc ttatattatc accaatcgtc accatcatta cttcatggtg atctttggaa
     4021 aggaaatatt ctttttcaaa aaaatggaga tcctattcta tgtgatccag tctgtttata
     4081 tggagataga gaatttgata ttggatatac tctagcatta gaaaaatttc cattagattt
     4141 ttatcaagaa tataataata tatatccgtt gtgtgttgga tatgaaaagc gaatagaatt
     4201 ttacaagtta tatgtgttta tgattcattt atttgctata ggtacagatt tttatgataa
     4261 agttaaagat accatgtata atattttaaa taattacggg gttgatttta taaaaggata
     4321 gggtgttgac tttgtttcaa ttttctaaaa tcatttatct acttctaata gtcacttttt
     4381 tcgaacgttt ataacgcaat catgatgaga gtattaatac agttaattat ttgaaatact
     4441 ttagtttata taatgttgaa ttataggtag tgaaattgta tttattatac attgtcatat
     4501 cgttgtttaa tgatagttaa tttaaggata atagaattaa tattatatat agatgaacaa
     4561 aacaagcaac agagcagaaa aaacactttt agattatttt tactcattct ctattttatt
     4621 tatttttgct ataataaata aataatatta aatggaggtg tttttatgaa aaagaaattt
     4681 gtttctagtt gtattgctag tacaatctta ttcggcactt tattaggtgt aacttataaa
     4741 gctgaagctg caacagtaca tgtagctggt ggtgtttgga gccatggtat tggaaaacat
     4801 tacgtatggt cttattatag tcacaataaa agaaatcatg gttcaacagc agtagggaaa
     4861 tattcatctt ttagtggtgt tgctcgacct ggtgttcaat ctaaggcatc tgctccaaag
     4921 gcttggggtg gaaacaaaac attttatagt cttcactaat tacatatatt aattaaggag
     4981 aatagacaga attaagtaaa cttaattctg tctattctct tgaaagaggc aatatgaaaa
     5041 aattcaaatt atttttagat tttagtactt tattattaat ttcagggcta ttatttttat
     5101 ttttcttcaa agaaaatgaa gaaattatac ctgaaagttc caatatttta acaatctcta
     5161 attgggataa atctaacagt aaatcaaaag tgttagatgt cattgaatca ggcgccaaaa
     5221 atcaaaatat acaaataatt aaatccgtta aagattttga taataaaaaa gaattttttg
     5281 ttttcaactc aaaacgaaat aattctgact ttatacgtaa taaaacatca cttttaactc
     5341 catcagattt attaaacaga gagataaaag gaaagtatta tattattggt gaacatttca
     5401 atgtagaaga gcttacgagt gaattaaaaa aagctggttt aacatctgaa gttgaataca
     5461 taaatagaaa catattattt tttgaattag tcatagataa tgatttatta gtgcctttca
     5521 tattcttagg cgtattatat cttttgtact tcttatatga cagaggatat aatttgaaat
     5581 tttatgctat tcaaaagcta catggctttt caactacacg cattattttt cataatattc
     5641 atataaaaat catttattgg ctagtactta ccgctatttt ctttattgct aatatattaa
     5701 tcattcat
//
LOCUS       DAMZLE010000350         5344 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.147, whole genome shotgun sequence.
ACCESSION   DAMZLE010000350 DAMZLE010000000
VERSION     DAMZLE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.147"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            237..1193
                     /locus_tag="POB92_002892"
     CDS             237..1193
                     /locus_tag="POB92_002892"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6580300.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1393..1872
                     /locus_tag="POB92_002893"
     CDS             1393..1872
                     /locus_tag="POB92_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI6580301.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVVGVDVIHSIEDIYQLPGHVFIFGGQILFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1887..2726
                     /gene="fakB2"
                     /locus_tag="POB92_002894"
     CDS             1887..2726
                     /gene="fakB2"
                     /locus_tag="POB92_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDI6580302.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2812..3345
                     /gene="msrA"
                     /locus_tag="POB92_002895"
     CDS             2812..3345
                     /gene="msrA"
                     /locus_tag="POB92_002895"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6580303.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3338..3766
                     /gene="msrB"
                     /locus_tag="POB92_002896"
     CDS             3338..3766
                     /gene="msrB"
                     /locus_tag="POB92_002896"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI6580304.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3778..4278
                     /locus_tag="POB92_002897"
     CDS             3778..4278
                     /locus_tag="POB92_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6580305.1"
                     /translation="MFKKLFGKGKEVQKDIAIYTPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4278..4499
                     /locus_tag="POB92_002898"
     CDS             4278..4499
                     /locus_tag="POB92_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDI6580306.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4691..>5344
                     /locus_tag="POB92_002899"
     CDS             4691..>5344
                     /locus_tag="POB92_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDI6580307.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     "
BASE COUNT         1995 a          766 c          949 g         1634 t
ORIGIN      
        1 ttttttaaat tttttagcat taaagttata gatttgggta aacaattacc aattggaaac
       61 atatatcacg ttacgatgtg gtaggtactt aatcagcatt ttataaataa agtaacggaa
      121 ttcatgatat taatatcata ttcctaaaat gagtgataac aaaatgctac ataaaattaa
      181 gttatatcaa actaaatata catactataa ataatgaaaa tgaggtgtta tcgcatatgt
      241 tgaattcatt tgatgcagca tatcacagtc tttgtgaaga agttttagaa ataggaaata
      301 cacgaaatga tcgcacaaat acaggtacga tttcgaaatt tggtcatcaa cttcgctttg
      361 acttatctaa aggatttcca ctattaacga caaagaaagt ttcttttaaa ttagtagcaa
      421 ccgaattatt atggttcatt aaaggagata caaacatcca atacttatta aaatataata
      481 ataatatttg gaacgaatgg gcttttgaaa attatataaa atcagacgag tataacggtc
      541 cagatatgac agatttcggg catcgtgcat tgagtgatcc tgaatttaac gaacaatata
      601 aagaacaaat gaaacaattt aagcaacgta ttcttgaaga tgatactttt gcgaagcaat
      661 tcggtgattt aggaaatgtt tatggtaaac aatggcgaga ttgggttgat aaagatggta
      721 atcattttga tcaacttaaa acagtaattg aacaaattaa gcataatcca gattcaagac
      781 gacacatcgt atctgcatgg aatccaacag aaattgatac aatggcactt ccgccttgtc
      841 ataccatgtt ccagttttat gtccaagatg gtaagttaag ttgccagtta taccaacgta
      901 gcgcagatat ctttttaggt gtgccattta atatcgcaag ctacgcttta ttgacacacc
      961 ttattgccaa agaatgtgga cttgaagtag gagaatttgt gcatacattt ggagatgcac
     1021 atatttattc aaatcatatt gatgcgattc aaacacaatt agcacgtgaa agcttcaatc
     1081 ctccaacatt aaaaattaac agtgacaagt ctattttcga cataaattat gaagatttgg
     1141 aaattgttga ctatgaatca catccagcaa taaaagctcc aatagcagtg tagtcattgc
     1201 atagttagct aaccatatag acatcaaaat gacatcatag tattttcaag tgcaaaaaag
     1261 tacttttttg tgttaaacgt tttcataaat tatgcaaaat cattatttct atcacacttt
     1321 atgataaaaa ttgtgttaaa ttaaagataa cttagtaata aaaaatgaaa tgatagaaga
     1381 aggaggataa ttatgacttt atccattcta gttgcacatg acttgcaacg agtaattggt
     1441 tttgaaaatc aattaccttg gcacctacca aatgatttga aacatgttaa aaaattatca
     1501 acaggtcata ctttagtaat gggtcgtaag acatttgaat cgattggtaa accactaccg
     1561 aatcgtcgaa atgttgtact tacttcagat acaagtttca acgtagtggg cgttgatgta
     1621 attcactcta ttgaagatat ttaccaacta ccgggccatg ttttcatatt tggagggcaa
     1681 atattatttg aagaaatgat tgataaagtg gacgacatgt atattactgt tattgaaggt
     1741 aaattccgtg gtgatacgtt ctttccacct tatacatttg aagactggga agttgcctct
     1801 tcagttgaag gtaaactaga tgagaaaaat acaattccac atacctttct acatttaatt
     1861 cgtaaaaaat aagggggaaa acgaccatga caaaacagat tatagtaaca gactcaacat
     1921 ccgatttatc taaagaatac ttagaagcaa acaacattca tgtaattcct ttaagtttaa
     1981 ctattgaagg agcttcatac gttgaccaag tagatattac atcagaagaa tttattaatc
     2041 atattgaaaa tgatgaagat gtaaagacaa gtcagccagc cataggtgaa tttatatctg
     2101 cttatgaaga actaggaaaa gatggctctg aaatcataag tattcatctt tcttcaggat
     2161 taagtggtac atataacact gcttaccaag caagtcaaat ggtagatgct aatgtaactg
     2221 ttattgattc aaaatctatt tcttttggtt tagggtatca aatacaacac ctagtagagc
     2281 ttgtaaaaga aggtgtctca acttctgaaa tagttaaaaa gttaaatcat ttaagagaaa
     2341 acattaaatt atttgtagtt atagggcaat tgaatcaatt aattaaaggt ggcagaatta
     2401 gtaaaacaaa aggtttgatt ggtaatctta tgaaaattaa accaattggt acactagatg
     2461 atggtcgctt agagcttgtg cacaatgcga gaactcaaaa ttctagtatc caatacttga
     2521 aaaaggaaat tgctgaattt ataggagatc atgaaatcaa atccattggt gtcgcacatg
     2581 ctaacgtcat tgaatatgtt gataaattga agaaagtttt taatgaagct tttcatgtga
     2641 ataattacga tataaatgta actacaccag ttatttctgc acatactggt caaggtgcga
     2701 ttggcctcgt agtccttaag aagtaaattt aatcttttca gtgttaatta cttccatttc
     2761 aatcctttat agactaaatt tataattaga tagatagagg aggtaattca tatgacaaaa
     2821 gaatatgcaa cattagcagg aggatgtttc tggtgcatgg ttaaaccatt tacatcatat
     2881 ccaggcatca agtcagtcgt atctggttat agtggcggtc atgttgacaa cccaacttat
     2941 gaacaggtat gtacgaatca aaccggccat gtcgaagcag tacaaattac gtttgatcca
     3001 gaggttactt cctttgaaaa tatattagac atatatttca aaacatttga cccaactgat
     3061 gatcaagggc aatttttcga tagaggcgaa agctatcaac cagtcatttt ctatcatgat
     3121 gaacatcaga aaaaggctgc tgagtttaaa aagcaacaat taaatgaaca aggtattttc
     3181 aagaaaccag tgattacacc tattaaacca tataaaaatt tctatccagc tgaagactac
     3241 catcaagatt attacaaaaa gaacccggta cattattacc aatatcaacg tggttcaggt
     3301 agaaaagcgt ttatagaatc acattggggg aatcaaaatg cttaaaaaag ataaaagtga
     3361 actaacagat atagaatata ttgttacaca agaaaacggc actgaaccac catttatgaa
     3421 tgaatattgg aatcattttg ctaaaggaat ttatgtagat aaaatttctg gtaaaccttt
     3481 atttacatct gaagaaaagt ttcattctga atgtggatgg cctagctttt ccaaagcgct
     3541 tgatgacgat gaaattatag aattagtcga caaatcattt ggtatgttga gaactgaagt
     3601 gcgttcagaa gaatcaaata gtcatttagg acatgtcttt aatgatggac ctaaagaaag
     3661 tggcggctta agatactgta tcaattccgc tgcaattcaa tttattccat atgaaaagtt
     3721 agaagaattg ggttatggcg atttaatatc acattttgat aagtaggagt ggaaagtatg
     3781 tttaaaaaat tattcggaaa aggcaaagaa gttcagaaag atatcgcaat ttatacacca
     3841 ttaactggag aattcgtgaa aattgaagat attccagatc ctgtattcgc acaaaaaatg
     3901 atgggcgaag gttttggtat taatccaact gaaggagaag ttgtgtctcc aattgcagga
     3961 cgtgttgaca atgtctttcc aactaagcat gcgattgggt taaaagcaga taacggatta
     4021 gaattattag ttcatatcgg tttagacaca gttcaattag acggtgaagg atttgaagtg
     4081 ttagtgtcta gtggtgacga agttaacgtc ggagatccat tagtaagatt caaccttgaa
     4141 tatattaata ataacgctaa atctgtaatt tcaccaatta taattactaa cactgatcaa
     4201 gcagcttcaa ttaatattta tgatgaaaat gctgtgatta aaggtgaaac aaaagtgatt
     4261 gatgtgacaa tgaactaatg aaaaactatt cgttttatca atttgtcatg acagttcgtg
     4321 gtcgacacga cgataaaggt cgtctagcag aagagatatt tgacgatctt gctttcccaa
     4381 aacacgatga tgattttaac atactgtctg attatattga gacacatggt gatttcacat
     4441 tgccaatgtc tgtatttgat gatttatatg aagaatatac ggaatggcta aaattttaat
     4501 ataattttta ataataatag ttagaaccag ggtgatgcaa ttcgttatcc tggttttaat
     4561 ttaaaataaa ctaagttgtg actaaaaatt aatcaattat agtgaaatat ggtgcgctat
     4621 cttgcataaa ttgatatgat taactacaca gaatttaaaa gtacataata cataataagg
     4681 aagtgataca atggatgata agcaacacac atcttcatcc gatgatgaac gcgctgaaat
     4741 tgcaacaagc aatcaagacc aagaaactaa ttcatcgaaa cgcgttcact taaaacgttg
     4801 gcaattcata tcaatattaa ttggtacaat cctaataaca gctgtcatca cagttgttgc
     4861 atatattttt ataaatcaaa aaataagtgg tttaaacaaa actgatcaag caaacttaaa
     4921 taaaattgaa aatgtgtata aaatcttaaa tagtgattat tacaaaaaac aggactctga
     4981 caagttaagt aaagctgcaa ttgatggcat ggtcaaagaa ttaaaagatc cttattctga
     5041 atatttaaca aaagaacaaa cgaaatcctt taatgaaggt gtttcaggtg attttgtagg
     5101 tattggtgca gaaatgcaaa agaaaaatga tcaaattatg gttactagtc ctatgaaggg
     5161 atctccagca gaacgtgctg gcattcgtcc taaagatgtc attactaaag taaatggaaa
     5221 atcaattaaa ggaaaagcat tagatgaagt tgtcaaagat gttcgtggta aagaaaacac
     5281 tgaagtcact ttaactgttc aacgaggtag tgaagaaaaa gacgttaaga ttaaacgtga
     5341 aaaa
//
LOCUS       DAMZLE010000351         3932 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.183, whole genome shotgun sequence.
ACCESSION   DAMZLE010000351 DAMZLE010000000
VERSION     DAMZLE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.183"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..309)
                     /locus_tag="POB92_002900"
     CDS             complement(<1..309)
                     /locus_tag="POB92_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580308.1"
                     /translation="MIKNKWKIEMWFRNFSYFIHKIDSEFILEEYLEDYLPKEYRPNF
                     ICGKAQPSETIYVSYFINQDEQTVEQHTVECDKTGFFIDEIPKHFIKNKMHYFIDSQS
                     K"
     gene            454..1158
                     /locus_tag="POB92_002901"
     CDS             454..1158
                     /locus_tag="POB92_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDI6580309.1"
                     /translation="MKRSVESFSELHQVLKNYRDNSLWLFRGHSNLNWSLVPKAGRAP
                     YDKYDDEETFYAWKRQAMIFLKETYSDWDMLTIAQHHGLATRLLDWSFNPLVAAFFAV
                     QTYEDCDAVIYAYLKKSDMNMKGATPFKSKWIKKVKPNGLASRVVRQSGIFTIHEPPT
                     NSIEANLSEKEELHKIIIKKHYRKKLREELSYYGINELSLFPDLDGLSSHVNWIMEIN
                     PYASNNITQELNSINE"
     gene            1182..2024
                     /locus_tag="POB92_002902"
     CDS             1182..2024
                     /locus_tag="POB92_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDI6580310.1"
                     /translation="MSVLETKLKSQMSKSAKIARNMNKLPDEIDRLRKRIERINKKRK
                     PTSSNIRDLEKSNKQLVTKQQKLADLQVEYTKIEKKINETKINLQKEQSRNQKKLSSM
                     LDKNTKGNEEIMEKLLTNSDQINEISNQIKKAVNQKEIIEYDVFLSHSSLDKEDYVSK
                     ISEKLIEKGLKVFEDVKVFEIGKSQTETMNMGILNSRFVVVFLSPNFIESGWSRYEFL
                     SFLNREINEEHVIILPIWHKVSVEDVRAYNPYLVDKYALNTSDFSIEEIVEKIYQVIV
                     NSKN"
     gene            2468..2776
                     /locus_tag="POB92_002903"
     CDS             2468..2776
                     /locus_tag="POB92_002903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580311.1"
                     /translation="MTEINESSLSLKTVYPVGTELSIDEYEIVKNKIMVLGKEKWTNL
                     LNEPHYYYLIEDFIETDYKKTSKGGLMGVKYFNVNEILNRDCLTTEQIAKELCNKDWE
                     "
     gene            2886..3044
                     /locus_tag="POB92_002904"
     CDS             2886..3044
                     /locus_tag="POB92_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6580312.1"
                     /translation="MYLKGVEGDTPKYAVPILETDFSDLPSTHTTVGSLDRFRDKTMS
                     INRSGSNC"
     gene            3362..>3932
                     /locus_tag="POB92_002905"
     CDS             3362..>3932
                     /locus_tag="POB92_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FusC family fusidic acid resistance EF-G-binding
                     protein"
                     /protein_id="HDI6580313.1"
                     /translation="MNKIEVYKFVKVKQLVYQLIKLYRTNDMNSHKTQKDFLLNEIND
                     IFKEKDIDISDFITSIDDVKLTKKKAEHLLNELKVYIQDFEIPSSSQLEKIFRKVKKL
                     KRPDINLIDTKEISYLGWNDNSSNRKYIVYKNLDDKFEGIYGEISPNKVKGFCKICNQ
                     ESDTSLFLNKTKHNKSSGTYTKKGDYICYD"
BASE COUNT         1569 a          456 c          662 g         1245 t
ORIGIN      
        1 tttactttga ctatcaataa aataatgcat tttatttttt ataaaatgtt tagggatttc
       61 atctataaaa aaaccagttt tgtcacattc cacagtatgt tgctccacag tttgttcatc
      121 ctgatttata aaataactta catatatagt ttcgctaggt tgggcttttc cacatataaa
      181 atttggtcgg tattctttag gtaaataatc ctctaagtat tcttctaata taaattctga
      241 atcaatttta tgtataaaat aactaaaatt ccgaaaccac atttctattt tccatttatt
      301 tttaatcata gtattactca ctttcaataa ttaaatgtat agacataatg ctagttttat
      361 ttttaagata tttcaataat gtttactaat ttttccctat aagataagat atacaatata
      421 aaagggaaca aacgttccta ttggaggggt cctatgaaaa ggtcagtcga gtctttcagt
      481 gagttacatc aagttttaaa gaattataga gataatagct tatggttatt tagaggtcat
      541 agtaatttga attggagttt agttccgaaa gctggtcgag caccttatga taaatatgat
      601 gatgaagaaa cattttatgc ttggaaacgt caagcaatga tatttttaaa agagacttat
      661 agtgattggg atatgctaac tatagcgcaa catcacggat tagctactag actattggat
      721 tggagtttta atcctttagt tgctgccttt tttgcggtgc aaacatatga ggattgtgat
      781 gctgttattt atgcctattt aaaaaaatcg gatatgaata tgaaaggggc tacgcctttt
      841 aagagtaaat ggataaaaaa agtaaagcct aacggtctag cttcgagagt ggttagacaa
      901 agtggaattt ttactattca tgagcctcct acaaattcta tagaagctaa tctttcagag
      961 aaagaagaac tacacaaaat catcataaag aaacattatc gcaaaaaatt aagagaagag
     1021 ttatcttatt atggtatcaa cgaattatca ttatttcctg acttagatgg tttatctagc
     1081 catgttaatt ggataatgga gataaatcca tacgcttcaa ataatataac gcaagaatta
     1141 aattcaatta atgaataaga ttataaaaag cgaggtatta tatgtcagta ttagaaacta
     1201 aattaaaaag tcaaatgtca aaatctgcaa aaatagctag aaatatgaat aagttaccag
     1261 atgaaattga tagattaaga aaaaggatag aaagaataaa taaaaaaaga aaacctactt
     1321 ccagtaatat tagagatttg gaaaaatcta ataagcaatt agttacaaag cagcaaaaac
     1381 ttgcagattt acaagttgag tatactaaga ttgagaaaaa aattaatgaa acaaaaatta
     1441 atcttcaaaa agagcagtct aggaatcaaa agaaattatc cagtatgtta gataaaaata
     1501 caaaaggaaa tgaagagatt atggaaaaat tattaactaa ttcagatcaa ataaatgaga
     1561 tatcaaatca gataaaaaaa gcagtgaatc aaaaagagat aattgaatat gatgtctttc
     1621 tatcccattc tagtttagat aaagaagatt atgtatctaa gataagtgaa aaactaatag
     1681 aaaaaggact aaaagtcttt gaagatgtta aagtttttga aatcggaaaa agtcaaacag
     1741 aaactatgaa tatgggtata ctaaactctc ggtttgttgt agtattttta tcccctaatt
     1801 ttattgaaag tggttggagc aggtacgaat ttttatcctt tctaaacaga gaaataaatg
     1861 aagagcatgt tattatatta cctatttggc ataaagtgag tgttgaagat gtacgtgcat
     1921 acaacccata tttagtagat aagtatgcat tgaatacaag tgattttagt atagaagaaa
     1981 tagtagaaaa aatttatcaa gtaatcgtta attctaagaa ttagtaatag tattggttat
     2041 ctactttaaa agcaaagagg gaattaaatg aaaaaagcag tgtttttatt actaaatgag
     2101 tatgcagaat ggtaagcaag ctactgttcc tctattttaa accaaagcag tgaatggaaa
     2161 atagaaacaa cttcaattta tgaatatttt acctcgattg gtggatttac aattaaagta
     2221 gtaatagtta tccaatattt tctagttgat tctcttataa tatgtccaat taagtgtaga
     2281 tgattcaaga tgatattgag ggaaggatga gaatgtgtct ttcaggtagc tcatttgatg
     2341 ataaccttaa acataatatt tactttcatc ctgctgaaaa agaggttaca gtttcaagcc
     2401 gatagaatac attgggttat ataaagacaa agcaataaaa gctatcggaa aagtagacaa
     2461 agtaatagtg acggaaataa atgagagctc attatccttg aagacagttt atcctgtagg
     2521 tactgagtta tcgattgatg agtacgaaat agttaaaaat aaaataatgg tgcttgggaa
     2581 agaaaagtgg actaatctat tgaacgaacc ccattattat tacttaattg aagattttat
     2641 agagacagac tacaagaaaa catctaaagg tggattgatg ggagttaagt attttaatgt
     2701 gaatgaaatt ttgaataggg attgcttaac caccgaacaa atcgctaaag aattatgtaa
     2761 taaagattgg gaatgataaa tagtgaatga agctataatg caataatgta tgtataacat
     2821 aatcaagtaa tgaatttaat gataagagag tatggagtaa agaattgaat tagtttgcat
     2881 ggtctatgta tcttaaaggt gttgaaggag atacaccgaa gtatgcagta ccaattcttg
     2941 aaacagactt tagtgattta ccatctacac atacgacagt cggatcactt gatcggtttc
     3001 gagataagac aatgagcatt aacagaagcg ggagtaactg ttgaattcca tttatatcca
     3061 gttagatact agagtttgaa gtgataaatc ttgattcgga tgctgaaata agtaaaagag
     3121 cagttactga atataatata agtataaata tgccactaga tattttaagg agtatttatt
     3181 cacttttata aaatcatctg ctaagatagt atggaagatt tttataggag ctgattataa
     3241 tgcgacatat aaatatgtca tacatcatac gctatagtac tctaactcaa agataagaag
     3301 tacttagtta tgaatagcat tcatttcaca aagtacttca acaaaaatgg aggaatatga
     3361 aatgaataaa atagaagtgt ataagtttgt taaagtaaag cagttagtat atcaattgat
     3421 taagttatat cgtacaaacg atatgaattc ccataaaaca caaaaagatt ttttactaaa
     3481 tgaaattaat gatatcttta aagaaaaaga tattgatatc tcggacttta ttacatcgat
     3541 tgacgatgta aaattaacta agaaaaaagc agaacatctt ttaaatgaat taaaagtgta
     3601 catccaagat tttgaaatac cttcatcaag tcaactggag aaaatttttc gtaaagtaaa
     3661 aaaattaaag agaccagata taaatttaat tgatacaaaa gaaatttcat atttaggatg
     3721 gaatgataat tcttctaacc gaaaatatat cgtttataaa aatttagatg ataaattcga
     3781 aggtatatat ggcgaaattt caccaaataa agtaaaagga ttctgtaaaa tttgtaatca
     3841 ggaatctgat acatcactct ttctcaataa aactaaacat aataagagta gtggaacata
     3901 tactaaaaaa ggagattaca tttgttatga ca
//
LOCUS       DAMZLE010000352         2513 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.232, whole genome shotgun sequence.
ACCESSION   DAMZLE010000352 DAMZLE010000000
VERSION     DAMZLE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.232"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..735
                     /gene="erm(C)"
                     /locus_tag="POB92_002906"
     CDS             1..735
                     /gene="erm(C)"
                     /locus_tag="POB92_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="HDI6580314.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALFLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
     gene            1183..1659
                     /locus_tag="POB92_002907"
     CDS             1183..1659
                     /locus_tag="POB92_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDI6580315.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
                     QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
                     GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
     gene            2354..2413
                     /gene="ermCL"
                     /locus_tag="POB92_002908"
     CDS             2354..2413
                     /gene="ermCL"
                     /locus_tag="POB92_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDI6580316.1"
                     /translation="MGIFSIFVISTVHHQPNKK"
BASE COUNT          981 a          380 c          392 g          760 t
ORIGIN      
        1 atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat
       61 aaaataatga caaatataag attaaatgaa catgataata tctttgaaat cggctcagga
      121 aaagggcatt ttacccttga attagtacag aggtgtaatt tcgtaactgc cattgaaata
      181 gaccataaat tatgcaaaac tacagaaaat aaacttgttg atcacgataa tttccaagtt
      241 ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa aatatttggt
      301 aatatacctt ataacataag tacggatata atacgcaaaa ttgtttttga tagtatagct
      361 gatgagattt atttaatcgt ggaatacggg tttgctaaaa gattattaaa tacaaaacgc
      421 tcattggcat tatttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga
      481 gaatattttc atcctaaacc taaagtgaat agctcactta tcagattaaa tagaaaaaaa
      541 tcaagaatat cacacaaaga taaacagaag tataattatt tcgttatgaa atgggttaac
      601 aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa acatgcagga
      661 attgacgatt taaacaatat tagctttgaa caattcttat ctcttttcaa tagctataaa
      721 ttatttaata agtaagttaa gggatgcata aactgcatcc cttaacttgt ttttcgtgta
      781 cctatttttt gtgaatcgat tatgtctttt gcgcattcac ttcttttcta tataaatatg
      841 agcgaagcga ataagcgtcg gaaaagcagc aaaaagtttc ctttttgctg ttggagcatg
      901 ggggttcagg gggtgcagta tctgacgtca atgccgagcg aaagcgagcc gaagggtagc
      961 atttacgtta gataaccccc tgatatgctc cgacgcttta tatagaaaag aagattcaac
     1021 taggtaaaat cttaatatag gttgagatga taaggtttat aaggaatttg tttgttctaa
     1081 tttttcactc attttgttct aatttctttt aacaaatgtt cttttttttt tagaacagtt
     1141 atgatatagt tagaatagtt taaaataagg agtgagaaaa agatgaaaga aagatatgga
     1201 acagtctata aaggctctca gaggctcata gacgaagaaa gtggagaagt catagaggta
     1261 gacaagttat accgtaaaca aacgtctggt aacttcgtaa aggcatatat agtgcaatta
     1321 ataagtatgt tagatatgat tggcggaaaa aaacttaaaa tcgttaacta tatcctagat
     1381 aatgtccact taagtaacaa tacaatgata gctacaacaa gagaaatagc aaaagctaca
     1441 ggaacaagtc tacaaacagt aataacaaca cttaaaatct tagaagaagg aaatattata
     1501 aaaagaaaaa ctggagtatt aatgttaaac cctgaactac taatgagagg cgacgaccaa
     1561 aaacaaaaat acctcttact cgaatttggg aactttgagc aagaggcaaa tgaaaaacaa
     1621 gaaaatgcat tatctgatta ttattctttc aaagactagt ataacataaa atcgtctaca
     1681 aatagacaaa aaacctgcac gcttaatgta gatcaaaagc ttaacgcaaa tgaaatagat
     1741 tgacctccca ataactacgt ggtgttattg ggaggtcaat ctatgaaatg cgattaagct
     1801 ttttctaatt cacataagcg tgcaggttta aagtacataa aaaatataat gaaaaaaagc
     1861 atcattatac taacgttata ccaacattat actctcatta tactaattgc ttattccaat
     1921 ttcctattgg ttggaaccaa caggcgttag tgtgttgttg agttggtact ttcatgggat
     1981 taatcccatg aaacccccaa ccaactcgcc aaagctttgg ctaacacaca cgccatccca
     2041 accaatagtt ttctcggcat aaagccatgc tctgacgctt aaatgcacta atgccttaaa
     2101 aaaacattaa agtctaacac actagactta tttacttcgt aattaagtcg ttaaaccgtg
     2161 tgctctacga ccaaaagtat aaaaccttta agaactttct tttttcttgt aaaaaaagaa
     2221 actagataaa tctctcatat cttttattca ataatcgcat cagattgcag tataaattta
     2281 acgatcactc atcatgttca tatttatcag agctcgtgct ataattatac taattttata
     2341 aggaggaaaa aatatgggca tttttagtat ttttgtaatc agcacagttc atcatcaacc
     2401 aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatta taaccaaatt
     2461 aaagagggtt ataatgaacg agaaaaatat aaaacacagt caaaacttta tta
//
LOCUS       DAMZLE010000353         2406 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.237, whole genome shotgun sequence.
ACCESSION   DAMZLE010000353 DAMZLE010000000
VERSION     DAMZLE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.237"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            199..906
                     /gene="deoD"
                     /locus_tag="POB92_002909"
     CDS             199..906
                     /gene="deoD"
                     /locus_tag="POB92_002909"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6580317.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVGTARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            913..2265
                     /gene="tet(38)"
                     /locus_tag="POB92_002910"
     CDS             913..2265
                     /gene="tet(38)"
                     /locus_tag="POB92_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6580318.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT          775 a          350 c          437 g          844 t
ORIGIN      
        1 actcgaattg gaaaaattat aaaaattaaa tatagatagg ctttgaaaat tagttttata
       61 caaggttagt agctgtaact gtaaaatgtt cttaatattg tcaaaatgta atgcttgaaa
      121 gcgcttttaa aaaatattat tatatacatg gttagacaaa tagacaaatc actatacaaa
      181 tattgggagg aatattttat gaaatcaaca ccacacatta aaccaatgaa tgacgtcgaa
      241 attgcagaaa cggttctatt gccaggagat ccgttaagag ctaagttcat tgcagaaact
      301 tatttggatg atgtggaaca gttcaataca gtgcgaaaca tgtttggttt taccggaaca
      361 tataaaggta aaaaagtttc tgtcatgggt tcaggtatgg gtatgccatc tattggcatt
      421 tactcttatg aattaattca tacatttggt tgtaaaaaat taattcgcgt tggttcttgt
      481 ggcgcgatgc aagaaaacat tgatttatat gatgtgatta ttgcacaagg tgcctctact
      541 gattcaaatt acgttcaaca atatcaatta ccaggtcatt ttgcgccaat tgcttcttat
      601 caattattag aaaaagcagt tggaacagca cgtgacaaag gtgtacgtca tcatgtaggt
      661 aatgtgttat caagtgatat tttctataac gcggatacaa cagcgagtga acgttggatg
      721 cgtatgggta ttttaggtgt agaaatggaa tcagctgcat tatacatgaa tgcaatttac
      781 gctggtgtcg aagcattagg tgtgttcaca gtgagcgatc atttaattca tgaaacgtca
      841 acaacacctg aggaaaggga acgtgcattt acagatatga ttgaaattgc actgtcattg
      901 gtttagataa ttatgaatgt tgaatattct aaaataaaga aagcagtacc tattttatta
      961 ttcttatttg tattcagttt ggttatagac aactcattta aattgatttc tgtagccatt
     1021 gctgatgact taaacatttc tgtaacgaca gtaagttggc aagcgacatt agccggttta
     1081 gtaattggta ttggcgctgt agtatacgct tcattatctg atgccattag tatacgcaca
     1141 ctatttattt atggcgtgat attaatcatt atcggatcaa ttattggtta cattttccaa
     1201 catcaattcg cattactttt agttggacgt attattcaaa ctgccggttt agccgctgca
     1261 gagacattat atgtgatata tgttgcaaag tatctttcta aagaggacca gaagacttac
     1321 cttggcttaa gtacgagcag ttattccttg tcattagtta tcggtacatt atcaggtgga
     1381 tttatttcta cgtatttaca ctggacaaat atgtttttaa ttgcattaat cgtagtattt
     1441 acgttgccat tcctatttaa attattacca aaagaaaata atacgaataa agctcattta
     1501 gattttgttg gcttaattct agtggcaact attgctacaa cagtcatgct gtttattacg
     1561 aactttaatt ggttatatat gattggtgcc ttaattgcga ttatcgtttt tgcgctatat
     1621 attaaaaatg cgcaacgtcc attagtaaat aaatcatttt tccaaaataa acgttatgct
     1681 tcatttttat ttatagtatt tgtaatgtat gctatccaat tgggttatat ttttacgttc
     1741 ccattcataa tggagcaaat ttatcatctg caactagaca caacatcact gttattagta
     1801 ccgggttata tagtagcagt catcgttggt gcactaagtg gtaaaatcgg cgaatatctg
     1861 aattcaaaac aagcgattat cacagcaatt attttaatcg cactgagctt gattttacct
     1921 gcatttgcag taggtaatca catttcaatc ttcgtcattt ctatgatatt ctttgcaggt
     1981 agctttgctt taatgtatgc acctttactt aacgaagcca ttaaaacaat agatcttaat
     2041 atgacaggtg tggctattgg tttttataat ttaattatta atgtggcggt atctgtaggt
     2101 attgcgattg ctgcggctct aatcgatttt aaagcattaa atttcccagg caatgatgca
     2161 ttaagttcac atttcggtat tattttaatt attttaggtt taatgagtat tgtcggatta
     2221 gttttattcg tcagcttaaa tcgttggaca caatctgaaa aataaataga tataaattcg
     2281 cgagatatat tcgtatttat agtaaaatta aataaagaga ttatataaca cgaggagtag
     2341 taagtatgaa atttgagaaa tatatagatc acactttatt gaagcctgag tcaacacgta
     2401 cgcaaa
//
LOCUS       DAMZLE010000354          410 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700998
            SAMN08111934-rid16878143.guided.340, whole genome shotgun sequence.
ACCESSION   DAMZLE010000354 DAMZLE010000000
VERSION     DAMZLE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111934
            Sequence Read Archive: SRR6332132
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591362.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 04:03:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,789
            CDSs (with protein)               :: 2,789
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 41 of 58
            Pseudo Genes (incomplete)         :: 20 of 58
            Pseudo Genes (internal stop)      :: 10 of 58
            Pseudo Genes (multiple problems)  :: 13 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111934-rid16878143.guided.340"
                     /isolate="DMG1700998"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            46..369
                     /gene="qacC"
                     /locus_tag="POB92_002911"
     CDS             46..369
                     /gene="qacC"
                     /locus_tag="POB92_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacC"
                     /protein_id="HDI6580319.1"
                     /translation="MPYIYLIIAISTEVIGSAFLKSSEGFSKFIPSLGTIISFGICFY
                     FLSKTMQHLPLNITYATWAGLGLVLTTVVSIIIFKEQINLITIVSIVLIIVGVVSLNI
                     FGTSH"
BASE COUNT          149 a           60 c           56 g          145 t
ORIGIN      
        1 ttagaataaa attaaataaa atacgaaaat taaaaggagt taaaaatgcc ttatatttat
       61 ttaataatag ccataagtac tgaagttatt ggaagtgcat ttcttaaatc ttcagaaggc
      121 ttttcaaaat ttataccatc cttaggaaca ataatttcat ttggaatttg tttctatttt
      181 ttaagtaaaa caatgcaaca cctaccacta aatataactt atgcaacttg ggcgggacta
      241 ggtttagtct taacaaccgt agtctcaata attattttca aagaacaaat aaatctaata
      301 actatagtat ctatagtttt aatcatagtc ggcgtagttt cgttaaacat tttcggaaca
      361 tcgcattaat tgctttattc caattgcttt attgacgttg agcctcggaa
//
